LOCUS       DASPZD010000001         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_238411, whole genome shotgun sequence.
ACCESSION   DASPZD010000001 DASPZD010000000
VERSION     DASPZD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238411"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..411
                     /locus_tag="VEM33_00005"
     CDS             184..411
                     /locus_tag="VEM33_00005"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HYS50244.1"
                     /translation="MSKPRQTYESDDISVTFDPDVCIHSGVCVRGLPDVFDIKRKRWI
                     RPELQPADVVAEQVMRCPSGALQFVRKQKTD"
     gene            423..707
                     /locus_tag="VEM33_00010"
     CDS             423..707
                     /locus_tag="VEM33_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYS50245.1"
                     /translation="MPTYEYKCPNGHLFEKTFPRITSKRHAKCPVCGKSAERLISGGA
                     GVHFKGSGFYATDYKRPAGASEKADSTAEKAEKTPKPDVKPPKKDSSSDK"
     gene            704..2347
                     /gene="argS"
                     /locus_tag="VEM33_00015"
     CDS             704..2347
                     /gene="argS"
                     /locus_tag="VEM33_00015"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYS50246.1"
                     /translation="MTALETIRTALAQAASRLGAPDVEVALERPRDATHGDLATNLAL
                     TLAKMLGQKPRAIAQRLVATIALPPGLVRKTEIAGPGFINFFLETAEVVGALPTILAA
                     GATYGRSAVGDRRPVNVEFVSANPTGPLHVGHGRQAALGDAIASLLEWTGWKVTREFY
                     YNDGGGQIANLARSVQARIAQLSGKSADIPEGGYHGEYIREIAERYVAAHQEDPAGKN
                     LDTVRRFAVDALREEQDRDLQAFGVRFDVYALESALYTSGKVDETEERLVAAGHTYEK
                     DGARWLRTTDFGDDKDRVMRKSAEKGGDYTYFVPDVAYHVTKWERGFSRAINVQGADH
                     HGTVRRVRVGLQALEMGIPEGYPEYVLHQMVTVMKGGEEVKISKRAGSYMTIRELVDE
                     VGRDAVRYFFLMRKSDTHFVFDVDLAKSQTEENPVFYVQMAHARMSGIFRVASREPDS
                     ATAAGVDLAVLTQPEEIELVNELADFPEMVQRAAAALEPNRITTYLEGLARLAHAWYH
                     KYRVLGEPEEAARLVLARAVRQVLANGLSLLGIRAPERM"
     gene            2350..>2617
                     /locus_tag="VEM33_00020"
     CDS             2350..>2617
                     /locus_tag="VEM33_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50247.1"
                     /translation="MGVLVVGSIALDSVITPFGETADALGGAAVFFAMAGAVLAPVQV
                     VGVVGSDFPEDHLKKLAARGVDLAGVERQPGESFRWKARYSYDLN"
BASE COUNT          504 a          790 c          890 g          433 t
ORIGIN      
        1 gccgacctga agcaggcgtt cgcaaacgcc gggaagaagt tcgacatggg caagtcatgc
       61 ctgcacttca cgcgccccga cgacctcgcg ctcgaggcga tcggcaaggt catcgcgagt
      121 tactcgcctg accagtggat cgcggtgatg gagaaaagcc ggcgccggaa gtagcttcgt
      181 gacatgtcca aaccgcgtca gacctatgag tccgacgaca tcagcgtcac cttcgatccc
      241 gacgtgtgca tccactcggg ggtgtgcgtc aggggattgc cggacgtatt cgacatcaaa
      301 cgcaaacggt ggatccggcc tgaactgcag ccggccgacg tcgtcgccga acaagtgatg
      361 cggtgccctt cgggcgccct ccaattcgtg cgtaagcaga agactgatta agttgtccgc
      421 cgatgccgac ctacgagtac aagtgcccaa acgggcacct gtttgagaaa acgtttcccc
      481 gcatcacgag taagcgccac gcgaaatgcc cggtatgcgg caagagcgcg gagcggctga
      541 tctcgggggg cgcgggcgtg catttcaagg gctccgggtt ctacgcgacc gattacaaac
      601 gaccggctgg agcgtcggaa aaggccgaca gtacggcgga gaaagccgag aagaccccga
      661 agcccgacgt gaagccgccc aagaaagact ctagctccga caagtgacgg cactcgagac
      721 gatccgtacg gcgctggccc aagcggcgtc acgattgggc gcgcccgatg tcgaggtcgc
      781 gctcgaacgt ccgcgcgacg cgacgcacgg cgatctcgcg accaacctcg ccctcacgct
      841 ggccaagatg ctcggtcaaa aaccgcgcgc catcgcgcag cggctcgtgg caacgatcgc
      901 gctgccaccc ggcctcgttc ggaagaccga aattgcggga ccgggcttca tcaacttctt
      961 cctcgagaca gccgaggtgg tgggtgccct gcccacgatc ctcgccgcgg gggcgacgta
     1021 cgggcgatct gcggttggag atcggcggcc cgtcaatgtc gaattcgtct cggcaaatcc
     1081 gacgggaccg ctgcatgtcg gtcatggccg tcaagcggca ttgggtgatg ccattgcctc
     1141 gctgctggaa tggaccggct ggaaggtgac gcgagagttc tactacaacg acggcggcgg
     1201 ccagatcgcg aacctcgcgc gcagcgtgca ggcgcgcatt gcgcagctca gcggcaagag
     1261 cgccgatatt ccggagggcg gttatcacgg cgagtacatt cgcgaaatcg cggaacgcta
     1321 cgtcgccgcg caccaagagg atcccgcggg aaagaatctc gatacggttc ggcgctttgc
     1381 cgtcgacgcg ctgcgcgaag agcaggatcg cgatctgcag gcatttggcg tgcggttcga
     1441 cgtctacgcg ctcgagtccg cgctctacac cagcggcaag gtcgatgaga ccgaagagcg
     1501 cctggttgcc gccgggcata cgtacgagaa ggatggcgcc cgctggctcc ggaccaccga
     1561 ctttggcgac gacaaggacc gggtgatgcg caagagcgca gagaagggcg gcgactacac
     1621 gtacttcgtc ccggacgtcg cgtatcatgt caccaagtgg gagcgcggtt tttcccgcgc
     1681 catcaacgta cagggcgcgg accaccacgg gacggtgcgg cgcgtgcgcg tggggctgca
     1741 ggcgctggaa atggggattc ccgagggtta tccggagtac gtgttgcatc agatggtaac
     1801 cgtcatgaag ggcggcgaag aggtaaagat ctccaagcgc gccggcagct atatgacgat
     1861 ccgcgagctg gtggatgaag tcgggcgcga tgcggtgcgc tacttctttc tcatgaggaa
     1921 aagcgacacg catttcgtgt tcgacgttga cctggccaag agccagaccg aagagaatcc
     1981 ggtcttctac gtccagatgg cgcacgcgcg catgagcggc atcttccgcg ttgcttcgcg
     2041 cgagccggac tcggcgacgg ctgcgggcgt cgatctggcg gtactcacgc agccggaaga
     2101 gatcgagctg gtcaacgagc tggccgactt cccggagatg gtgcagcgcg cagcggctgc
     2161 gctggagcca aatcgcatca cgacttatct cgagggactg gcccggttgg cgcacgcgtg
     2221 gtatcacaag taccgcgtgt tgggggagcc ggaggaagcg gcccggctcg tgctcgcgcg
     2281 cgcggtcaga caggtgctcg cgaatggcct ttcactcttg gggattcgcg ccccggaacg
     2341 gatgtgagcg tgggtgtact cgtcgtcggc tcgatcgcgc tggattccgt catcacgccc
     2401 ttcggcgaga ccgccgacgc gctcggcggg gccgcggtgt tctttgcgat ggcgggagcg
     2461 gtgctggctc ccgtccaggt cgtcggcgtg gtcgggagcg actttcccga agatcatctc
     2521 aagaaactgg cggcgcgcgg cgtggacctg gcgggcgtgg agcggcaacc gggcgagtcg
     2581 ttccgctgga aggcgcgcta ctcctacgac ctcaata
//
LOCUS       DASPZD010000002         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_962037, whole genome shotgun sequence.
ACCESSION   DASPZD010000002 DASPZD010000000
VERSION     DASPZD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962037"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VEM33_00025"
     CDS             <1..505
                     /locus_tag="VEM33_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HYS50248.1"
                     /translation="QSLAQCHAWLSQHLPGAQRVPQASNAEAARMAAKEKSAAAIGPE
                     IAAERYRLKVLARAIEDDPRNRTRFLVLGSHDAAPSGKDRTSLVMTTHNRPGSLHELI
                     ASFSTHGVNMTRLESRPARTGQWEYYFYVDIEGHQQDPKAAKALAELRDKAPFVKIFG
                     SYPAAVL"
     gene            515..1615
                     /gene="hisC"
                     /locus_tag="VEM33_00030"
     CDS             515..1615
                     /gene="hisC"
                     /locus_tag="VEM33_00030"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYS50249.1"
                     /translation="MDLCEQSPSYIRAIAPYQPGKPIAELAREMGLKEKNIIKLASNE
                     NPFGISPRAKAAVKRGLAELARYPDGNAFELKAALSRRHGVPEECVVVGNGSNDLLEL
                     AAGAFLAPGRAAVYSQHAFAVYPLATQARGATGIVVPAKNYAHDFEAMLAAITPETRL
                     VFIANPNNPTGTFATGAELENFIARVRRDVAVVVDEAYSEYLPPELRYDSVALLKEHP
                     NLIVTRTFSKVYGLAGLRVGYGLMHPDVADLLNRVRQPFNVNSLALAAAAAALEDRKF
                     VAKSCRMNRTGMKSLERGLKALGLGFIPSCANFLTFRVSRARTVYEKLLRLGVIVRPL
                     ANYDMPDHLRVTVGTPKENEKFLKALRTALDD"
     gene            1615..2487
                     /locus_tag="VEM33_00035"
     CDS             1615..2487
                     /locus_tag="VEM33_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HYS50250.1"
                     /translation="MVRIGKLVVVGVGLIGGSFALSLKEAGAVGRAVGVGRSGRNIRR
                     ALDLKIIDAAGAFDRATFADADLALLAVPVGQMRPVMRAIAPLIGAKTVVTDAGSTKQ
                     DVVGLARRELKAALSRFVPGHPIAGTEKSGAEAAFAGLYRGRRVVLTPLKGTERGALA
                     LVRSAWERCGASVSELEPREHDAVLAAVSHLPHVLAYALVDQVARAKNAKQLFSFAAG
                     GFRDFTRIASSHPEMWRDICIANRKALLVELRRYGGELERVEKMLERGDARALEALFS
                     GARGARERWLKDTQ"
     gene            2665..>3919
                     /gene="aroA"
                     /locus_tag="VEM33_00040"
     CDS             2665..>3919
                     /gene="aroA"
                     /locus_tag="VEM33_00040"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYS50251.1"
                     /translation="MKFLDLEPIARVAGTVKMPGSKSISNRVLLLAALAEGKTRVENL
                     LDSDDTGVMLNALELLGVRIERDPATVRGGPFPNKSADLHLGNAGTAFRPLTAVLALA
                     GGEYRLSGAPRMHERPIGDLVDALRALGARIDYLGKEGFPPLALHSGTIRRGASVRVR
                     GDVSSQYLSALLMAAPLAGSETRIEVEGELISKPYVEITLDIMRRFGVEVQREGWRSF
                     VVPGKRYSSPGKIRVEGDASSASYFLAAGAIGGGPVRVEGVGRSSIQGDVRFTEVLEK
                     MGAKISMGEDWIESGAPARGKLKAVDADLNHIPDAAMTAAVAALFADGPSRIRNIASW
                     RVKETDRIAAMAAELRKLGAAVEEGADALRVTPPARLKPATIATYGDHRMAMSFSLAA
                     LGGIRVRIGDPDCVAKTFPEYFEALA"
BASE COUNT          633 a         1338 c         1362 g          586 t
ORIGIN      
        1 gcagtcgctc gcgcagtgcc atgcctggct ctcccagcat cttccgggtg cccagcgcgt
       61 gccgcaagcg agcaacgcgg aagcggcgcg catggcggcg aaggagaaga gcgccgccgc
      121 gataggcccg gagatcgccg ccgagcgcta caggctcaag gtgctcgcgc gcgccatcga
      181 ggacgatccc aggaaccgca cgcgcttcct tgtgctgggg tcccacgacg cggcgccctc
      241 gggcaaggac cgcacctcgc tcgtcatgac cactcacaac aggcccggct ccctgcacga
      301 gctgatcgct tccttctcga cgcacggcgt caacatgacg cgcctcgagt cccggcccgc
      361 gcgcaccggc cagtgggagt actacttcta cgtcgacatc gaaggccacc agcaggatcc
      421 caaggcggcc aaggcgctcg ccgagctgcg cgacaaggcg ccgttcgtga agatattcgg
      481 cagctatccc gctgccgttc tctaggcacg ctccatggac ctgtgcgagc agtcgccgtc
      541 ttacatccgc gccatcgccc cgtaccagcc gggcaagccg atcgcggagc tcgcccgcga
      601 aatggggctg aaagagaaga acatcatcaa gctcgcgtcg aacgagaacc cgttcggcat
      661 ctcgcccagg gcgaaggccg cggtcaagag agggctcgct gagctcgcgc gctaccccga
      721 cggcaacgcg ttcgagctga aagccgcgct ctcgcgccgc cacggcgttc cagaggagtg
      781 cgtcgtcgtc ggcaacggct cgaacgacct gctcgagctc gcggccggcg ccttcctggc
      841 cccgggccgc gcggcggtgt actcgcagca cgcctttgcc gtctatccgc tcgcgaccca
      901 ggcgcgcggc gcgacgggca tcgtcgtgcc ggcgaaaaac tacgcccacg acttcgaggc
      961 gatgctcgcc gcgatcacgc cggagacgcg cctggtgttt atcgccaatc ccaataaccc
     1021 caccgggaca ttcgcgacgg gcgcggagct ggagaatttc atcgcccgcg tgcggcgcga
     1081 cgtggccgtg gtggtggacg aggcctactc cgagtacctg ccgcccgagt tgcgctacga
     1141 cagcgtggcg ctgctgaagg aacacccgaa cctcatcgtc acgcgcacct tctccaaggt
     1201 ctacggactc gcggggctgc gcgtcggata cgggctgatg cacccggacg tcgcggacct
     1261 cctcaaccgc gtgcgccagc ctttcaacgt caacagcctc gcgctcgccg ccgcggccgc
     1321 agcgctcgag gacaggaaat tcgtggcgaa aagctgcagg atgaaccgca cgggcatgaa
     1381 gagcctcgag cgcggcctga aggcgctcgg gctcgggttc atcccctcgt gcgccaattt
     1441 cctcacgttc cgcgtgtcgc gggcgaggac ggtgtacgag aagctgctgc gcctgggtgt
     1501 catcgtcagg ccgctggcga actacgatat gcccgatcat ttgcgagtca ccgtcggcac
     1561 gccgaaagag aacgagaagt tcctcaaggc gctgagaacg gcgctcgacg attgatggtg
     1621 cgcatcggga agctggtggt cgtcggcgtc ggcctgatcg gcggctcgtt cgcgctctcg
     1681 ctgaaagagg cgggcgcggt cggccgcgcg gtcggcgtcg gccgcagcgg caggaatatc
     1741 cggcgcgcgc tcgacctgaa gatcatcgac gccgccggcg cattcgaccg cgcgacgttc
     1801 gcggacgccg atctcgcgct gctcgcggtg ccggtcggac agatgcgccc ggtgatgcgc
     1861 gccatcgcgc cgctcatcgg tgcaaagacc gtcgtcaccg acgcgggaag caccaagcag
     1921 gacgtggtcg ggctcgcccg gcgcgagctg aaagccgcgc tttcccgctt cgtcccgggg
     1981 catccgatcg caggcaccga aaaaagcggc gccgaagccg cgttcgccgg gctctatcgc
     2041 gggcgcaggg tggtgctgac gccgctcaag ggaaccgagc gcggcgcgct cgccctggtg
     2101 cgctccgcgt gggagcgctg cggggcgagc gtttccgagc tcgagccgcg cgagcacgac
     2161 gcggtgctcg cggcagtcag ccatctgccg cacgtgctcg cctacgcgct cgtcgatcag
     2221 gtcgcgcgcg cgaagaacgc gaagcagctc ttttccttcg cggcgggagg attccgcgat
     2281 ttcacccgca tcgcgtccag ccacccggag atgtggcgcg acatctgcat tgccaaccgc
     2341 aaggccctgc tcgtcgagct caggcgctat ggcggggagc tcgagcgcgt ggagaagatg
     2401 ctcgagcgcg gcgacgccag ggcgctcgag gcgctcttct cgggcgcacg cggcgcgcgc
     2461 gagcgctggc tgaaggacac gcaatgagag cagcgcctgg attttcccct cctctttcga
     2521 ggaggggtgc cgccgaaggc ggcggggtgg tgtcgccgcg agaaggatgg tcgaccaccc
     2581 cgtcgcgctg cgcgcgccac ccctccttgt ccaaggaggg gaaaaagacc gctggggcgc
     2641 ggttcgcctg agagcgcgcg taccgtgaaa ttcctcgatc ttgagccgat cgcgcgcgtg
     2701 gcgggcaccg tgaagatgcc ggggtcgaag agcatttcga accgcgtgct gctgcttgcc
     2761 gcgctggcgg agggcaagac gcgcgtcgag aatctgctgg attccgacga caccggcgtg
     2821 atgctgaacg ccctcgagct tctaggcgtc cgcatcgagc gcgatccggc cacggtgcgc
     2881 ggcggcccgt tcccgaacaa gtccgccgac cttcacctcg gcaacgccgg cacggcattc
     2941 cggccgctta ccgccgtcct cgcgctcgcg ggcggcgagt accggctctc gggcgctccg
     3001 cggatgcacg agcggccgat cggcgacctg gtcgacgcct tgcgagcgct cggcgcgcgc
     3061 atcgattatc tcggcaagga aggctttccg ccgctcgcct tgcactccgg cacgatccgg
     3121 cggggcgcgt cggttcgcgt gcgcggggat gtgtcctcgc agtatctctc ggcgctgctg
     3181 atggctgcgc cgctcgcggg atcggaaacg cggatcgaag tcgagggcga gctgatctcc
     3241 aagccctacg tcgagatcac cctggacatc atgcgacgct tcggcgtcga ggtgcagcgc
     3301 gagggctgga ggtcgttcgt ggttccgggc aagcgctact cgagccccgg gaagatccgc
     3361 gtcgaagggg acgcctcctc ggcctcgtat ttcctcgcgg cgggcgcgat cggcgggggc
     3421 ccggtacggg tggaaggcgt ggggcgttcg agcatccagg gcgatgtccg cttcaccgag
     3481 gtgctggaga agatgggggc gaagatctcg atgggcgagg actggatcga atccggcgcg
     3541 ccggcccggg gaaagctgaa agccgtcgac gcggacttga accacatccc cgacgcggcg
     3601 atgaccgcgg cggtcgcggc gctcttcgcc gacggaccga gcaggatccg caatatcgcc
     3661 agctggcgag tcaaggaaac cgaccgcatc gccgcgatgg cggccgaatt gcgcaagctc
     3721 ggcgcggcgg tcgaggaggg cgccgacgcc ctgcgcgtga cgcctccggc gcgcctgaag
     3781 cccgcgacga tagccaccta cggcgaccac cgcatggcga tgtccttttc cctcgcggcg
     3841 ctcgggggga tcagggtgcg catcggcgac ccggactgcg tggcaaagac cttcccggaa
     3901 tacttcgagg cgctcgcgg
//
LOCUS       DASPZD010000003         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1203383, whole genome shotgun sequence.
ACCESSION   DASPZD010000003 DASPZD010000000
VERSION     DASPZD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203383"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1224)
                     /locus_tag="VEM33_00045"
     CDS             complement(106..1224)
                     /locus_tag="VEM33_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYS50252.1"
                     /translation="MNRTDFLIALIGISALVAGCEKGATAVTAPASKASRDPGVRSAQ
                     IADAQKQYLSIETVAVSQAVDLLALPGRVTFRPQAQSAIGATTAGRVVAVLVQAGQVV
                     KAGTELLTIESADAGTARATLDQAATRLGSAESVFRRQVEMVEKGVGLEFERQEAEAR
                     LKEARAEYERARNAADLIGSGQGIRVTVRAPADGVVITIRTAVGATVAPGGEALLELG
                     DPTRLQVVAQVPEGDLRRIAVGQEADVELPALAARVAARVENFNPRVDPESRRSQVYL
                     ALSKRIDGLRAGMLAQVALRVGAKADVLVPVSAVLIKDGKRRVVYVEREDGSFEAREV
                     QTGRNQDGQVVILQGLTAGERVVVRGALLLDTQAEQLL"
     gene            1375..2403
                     /gene="tsaD"
                     /locus_tag="VEM33_00050"
     CDS             1375..2403
                     /gene="tsaD"
                     /locus_tag="VEM33_00050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYS50253.1"
                     /translation="MLVLGIETSCDETGVAVYDSGRGLLAHALHSQVELHDAYGGVVP
                     ELASRDHIRRLLPLVREVLRKAGAQGSDLDAVAYTQGPGLAGALLVGAAVANSLAFGL
                     GVRAIGVHHLEGHLLSPLLEKKAPPFPFVALLVSGGHTQLMRVADVGDYEMLGETLDD
                     AAGEAFDKTAKLLGLGYPGGPALAKLAQEGRADRFAFPRPMLEQLEKNGDLNFSFSGL
                     KTAVVALTRTHALDEQTRADVAAAFQEAVADVLVAKSLAAVRRTALERLVVAGGVGAN
                     VRLRSLLDAAASRGKFSVFYPAMEFCTDNGAMIALAGCLRLKTAKAAPALPPGFTVRP
                     RWDLASLR"
     gene            complement(2400..>2589)
                     /locus_tag="VEM33_00055"
     CDS             complement(2400..>2589)
                     /locus_tag="VEM33_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HYS50254.1"
                     /translation="VSAVFAPLYAFFMSLLGFEPGSMLSAIILIAALLVWRHKTNILN
                     LVAGKERRIGERKTGEAA"
BASE COUNT          450 a          884 c          801 g          454 t
ORIGIN      
        1 gcgtgtcgag gtaggcgcgc agtccaaagg ccgcgacgac caaggtggcg aacaacgccg
       61 cgacccggcg gtgaacgcag gtctcgatca ggaggcggag catggtcaga gcagctgctc
      121 tgcctgggtg tcgagcagca aggcgccgcg caccaccacc ctttcgcccg ccgtgagccc
      181 ctgcagaatc accacctgac cgtcttggtt gcggcccgtc tgtacctcgc gggcctcgaa
      241 gctgccgtcc tctcgctcca cgtagaccac gcggcgtttt ccgtccttga tcagcaccgc
      301 cgagaccggg accaagacgt ccgccttggc gcccacgcgc agggcaacct gggccagcat
      361 gccggcccgc aaaccatcga tgcgcttgga aagcgccaga tacacctggc tgcgacgcga
      421 ttccggatcc acgcgagggt tgaaattctc gacccgtgct gcaacacggg cagcgagcgc
      481 cggcagttcc acgtccgctt cctggccgac ggcaattcgg cgcaaatcgc cttccggaac
      541 ctgcgcgacc acctgcagac gggtcggatc accgagctcc aataacgctt cgccgccagg
      601 cgcgaccgtg gcgccgaccg cggtgcgaat cgttatcacc acaccgtccg cgggtgctcg
      661 aacagtaacc cgtattcctt gaccggagcc gattaagtcc gcggcgttcc gcgcccgctc
      721 atattccgcg cgcgcttcct tcagccgcgc ttcggcttct tgccgctcga actccaggcc
      781 gacgcctttt tccaccatct cgacctggcg cctgaagacg ctctcggcgc tgcccagcct
      841 cgtggctgcc tgatcgagcg tagcgcgcgc ggttcccgcg tcggcgcttt cgatggttaa
      901 gagttccgtc ccggccttga ctacttgtcc ggcctgaacc agcacggcaa cgacgcgccc
      961 cgccgtcgtg gctccgatag cggattgtgc ctgcggacgg aacgtcacgc gacccggcaa
     1021 cgcgagcaga tcgacggcct gggaaacggc gacggtctcg atggagagat actgcttctg
     1081 ggcgtccgct atctgcgccg agcggacgcc gggatcacga gaagccttgg acgcgggcgc
     1141 cgtgacggct gtcgccccct tctcgcatcc agccaccagc gctgaaatcc cgatcagcgc
     1201 aataagaaaa tccgttcgat tcatacccga taaaccttag tttcctcata gactccggtt
     1261 tcccgctcga agcacgcacg aggacccgta ttcgtgcaaa tatctgaggg cgcgatttta
     1321 gaggaacatg ctgactgccg gctgacacga cagtccgaag aacgctgaaa aatcatgctt
     1381 gtcctgggca tcgagacctc ctgcgacgaa accggggtgg ccgtctacga cagcggccgc
     1441 ggcctgctcg ctcacgcgct gcattcgcag gtggagctgc acgacgcgta cgggggcgtc
     1501 gtgccggagc tcgcgtctcg ggaccacatt cggcgtctgc tccctctcgt ccgcgaagtc
     1561 ctgcgcaaag ccggtgccca agggtcggac ctcgacgcag tcgcctacac ccagggcccg
     1621 ggcctcgcag gagcgctgct cgtcggcgcc gctgtcgcga acagcctcgc gttcggccta
     1681 ggagtccggg cgatcggcgt gcatcatctc gaggggcatc tgctttcgcc tttgcttgaa
     1741 aaaaaggcac cgccattccc gtttgtcgcg ctgctggttt cggggggcca cactcaactt
     1801 atgcgcgtgg cggatgttgg tgactacgaa atgctggggg aaaccctgga cgacgccgcc
     1861 ggggaggctt tcgacaagac tgcgaaactg ctcggcctcg gctaccccgg agggcctgcg
     1921 ctcgccaagc tcgcgcaaga gggcagggcc gatcgctttg cgtttccgcg cccgatgctc
     1981 gagcagttgg agaaaaacgg cgatctcaat ttcagcttca gcgggctcaa gaccgcggtt
     2041 gtagcgctca cgcgcacgca cgcgctcgac gagcagacgc gcgccgacgt cgccgcggcg
     2101 ttccaggagg ccgtggccga cgtcctggtt gcgaaatcgc tcgccgcggt gcgcagaacg
     2161 gccctcgaac gtcttgtcgt ggcggggggc gtcggcgcca acgttcgcct gcgctccctg
     2221 ctcgatgccg ccgcttcgcg cggtaaattc agcgtgttct acccggcgat ggaattctgc
     2281 accgacaacg gcgcgatgat cgcgctggca gggtgcttgc gactgaaaac cgccaaggct
     2341 gctccggcac ttccacccgg attcaccgtc aggccgcgct gggacctggc ctccctgcgc
     2401 taagcggctt cgccggtctt tctctcgccg attcggcgct ccttcccggc gacgaggttg
     2461 aggatgttgg tcttgtggcg ccacaccagc agcgccgcta tcaagattat cgcggacaac
     2521 atcgagcccg gctcgaatcc gagcagcgac atgaaaaatg catagagcgg tgcaaatact
     2581 gccgagacg
//
LOCUS       DASPZD010000004         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1802903, whole genome shotgun sequence.
ACCESSION   DASPZD010000004 DASPZD010000000
VERSION     DASPZD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802903"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VEM33_00060"
     CDS             complement(<1..530)
                     /locus_tag="VEM33_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50255.1"
                     /translation="MRARLARREALGLTRAEFLVLTRLSTPEKIQGFLDALPSNFEIG
                     GQTCLSVREVLRRRRAHCIEGAMLAACALWVHGEPPLLMDLRAERDYDHVVVLFRRNG
                     HWGAISKTNPPVLRWRDPVYRTLRELAMSYLHEYANDRAQKTLRSYSRPFDLRRLDPR
                     IWVTNGKNCWDVGEAV"
     gene            600..1097
                     /locus_tag="VEM33_00065"
     CDS             600..1097
                     /locus_tag="VEM33_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HYS50256.1"
                     /translation="MIRLLLSRRIGNLVGFLVCAGMLGFGYYLQFVVGLEPCPLCIIQ
                     RILLFAVGVAFLVAALHHPAGRLAAGLYGGVVASIAAIGIAVAGRHVWLQHLPPDKRP
                     ACGPALDYLLSAFGPVEGLSRVLRGSGECGAVDWTLLSFSIPEWTLAAFIALSIWAVF
                     LALRD"
     gene            complement(1094..1330)
                     /locus_tag="VEM33_00070"
     CDS             complement(1094..1330)
                     /locus_tag="VEM33_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50257.1"
                     /translation="MSSRPIPETVWRSPSGEVVSCIEKLKVLRENLEEIQQICQGALE
                     DAVLMGCDESQFRQILGRLVEQIENPYRDSRSDA"
     gene            complement(1397..>2121)
                     /gene="cobA"
                     /locus_tag="VEM33_00075"
     CDS             complement(1397..>2121)
                     /gene="cobA"
                     /locus_tag="VEM33_00075"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYS50258.1"
                     /translation="DLLTLRAAEILERAELVFHDALVHPQTLALAAHARKILVGKRSG
                     RHSTAQSFINKRLVDAARKHAVVVRLKGGDPMLFGRAQEEIDALRKAGIDFEIVPGVT
                     AATAAGAELGVSLTRRGLARHVAFVTPRAGRGERENDWAASVLAADTAVLYMGAGLAE
                     AISAELIGRGKPKATPLVLVESATLPERRILRGTLADLPALALQAGSGPAVLLLGEVL
                     RDALADRAVVAIPEIQAYLRRA"
BASE COUNT          317 a          712 c          695 g          397 t
ORIGIN      
        1 tcgaccgcct cgcccacgtc ccaacagttt tttccattgg tgacccagat cctgggatcc
       61 agcctgcgca ggtcgaaagg gcgcgagtag ctgcgaaggg tcttctgggc gcgatcgttc
      121 gcgtactcgt gcaggtagga catggctagc tcgcgcaggg tgcggtacac gggatcgcgc
      181 cagcgcagca cgggcggatt ggttttcgag attgctcccc aatgaccgtt gcggcgaaac
      241 agcacgacca catggtcgta gtcgcgctct gcgcgcaggt ccatgaggag cggcggctcg
      301 ccatgcaccc acagcgcgca ggctgcgagc attgcgcctt cgatgcagtg tgcccggcgc
      361 cggcgcagca cctcgcgcac cgagaggcag gtctggccgc cgatctcgaa attggagggc
      421 agcgcgtcga gaaacccctg gattttctcc ggagtggaaa gccgtgtcag aaccaggaac
      481 tcggcacggg taaggcccag ggcttctcgg cgggcaagac gcgctctcaa ctctcgactc
      541 cttgaaaccg ggggttatgg ggtgctgctt caggcattct aatgctaggc tcttcctcca
      601 tgatcagact cttgctttcc cggcggatcg gaaacctcgt cgggttcctc gtctgcgccg
      661 gcatgctcgg cttcggctat tacctgcagt tcgtcgtcgg cctcgagccc tgtccgctgt
      721 gcatcatcca gcggatcctg ctttttgcgg tcggcgtcgc gttcctcgtc gccgcccttc
      781 atcacccggc gggcaggcta gcggcggggc tctacggcgg ggtcgtcgct tccatcgcgg
      841 caatcggcat cgcggtcgcg gggcggcacg tgtggctgca gcacctgccg ccggacaagc
      901 gccccgcatg cggccccgcg ctcgactatc tgctctcggc tttcggccca gtcgaggggt
      961 tgagtcgcgt cttgcgcggc tcgggcgaat gcggcgcggt cgactggaca ctgctctcat
     1021 tcagcatccc cgagtggacg ctcgccgcgt tcatcgcgct gtcgatctgg gctgtttttc
     1081 tcgcgttgcg ggactaggcg tcgctccgac tgtcgcggta gggattttcg atctgctcga
     1141 ccagcctgcc gaggatttgt ctgaactggc tctcgtcgca gcccatcagc accgcgtcct
     1201 cgagcgcacc ctggcagatc tgctggatct cctccagatt ctcccggagc actttcagtt
     1261 tttcgatgca ggacacgact tcgcctgaag gcgagcgcca gacggtctcc gggatggggc
     1321 gtgagctcat gtgcagaacc tcgggaaacg aataccgatc tgaccccgca aaccgaaacg
     1381 ggttcgcgcc ggcggctcag gcccgcctga gataagcctg gatttccggg atcgcgacga
     1441 cggcgcggtc cgcaagcgcg tccctcagca cttcgccgag gagcagcacc gcggggccgc
     1501 tgccggcctg aagcgccagc gcgggcaggt ctgccagcgt tccgcggagg atgcgccgct
     1561 cagggagcgt cgcgctttcc accaggacga gcggcgtggc cttcggcttg ccgcgcccaa
     1621 tcagctctgc gctgatcgct tcagcaagcc ccgcgcccat gtagaggacc gccgtgtcgg
     1681 ccgcgagcac gctcgccgcc caatcgttct cgcgttcgcc ccgccccgcg cgcggcgtga
     1741 cgaaagcgac atggcgggca aggccgcggc gcgtgagcga taccccgagc tctgcgcccg
     1801 cggcggtcgc ggcggtcact cccgggacga tttcaaaatc gattccggct ttgcgcagcg
     1861 cgtcgatttc ctcctgcgcg cggccgaaga gcatggggtc gcctcccttc agccgcacca
     1921 cgacagcgtg cttgcgggcg gcatccacca gcctcttgtt tatgaagctt tgtgccgtcg
     1981 aatggcggcc gctgcgcttg cccacgagaa ttttccgggc gtgcgcggcg agcgcgagcg
     2041 tctgcgggtg caccagcgcg tcgtggaaga ccaactcggc gcgctcgaga atttccgccg
     2101 cccgcagagt gagcaggtcg g
//
LOCUS       DASPZD010000005         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1563869, whole genome shotgun sequence.
ACCESSION   DASPZD010000005 DASPZD010000000
VERSION     DASPZD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563869"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1146)
                     /locus_tag="VEM33_00080"
     CDS             complement(136..1146)
                     /locus_tag="VEM33_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50259.1"
                     /translation="MKNLVIDIHAHFIPKLLYGRYDANASKFPGVRLLRDAKGTRMQF
                     PGGEPTRPISPKLSDTADRRAWMDRNGIDHQLVGGWLDSFGYELPSGEGLAWSRFYND
                     CLKEELREEKRFTPLATVPLQHGGLAAEVLGEALDKGFGGVMIGTLPKGNAGNLDDPA
                     LDPFWQTASDLKAAVFMHPMFLCGEPRLADYDLVNAIGRLADTSIAVSRLLFSGHLLK
                     FPGMKFVLSHGGAALPYALGRLARNHANSQGKYADPRKGFEAMYFDSCVFDADSLEYL
                     AKKAGSARIMLGSDQPFPIGDPEPKKIVEGATFNEAQRKGILGDTAQSVFRVRPDCWC
                     PR"
     gene            complement(1149..>2116)
                     /locus_tag="VEM33_00085"
     CDS             complement(1149..>2116)
                     /locus_tag="VEM33_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYS50260.1"
                     /translation="DSCLVRVDLSGSITGIINNSSAGQGHESLVSTVLGEILERDPAS
                     IRVVRADSLTALPSNSPVGSRMAIMLGGAAAGAAKQIKETLIAIAAHNFECSPEDLDY
                     RDGSVSLKGAPEKKLAWDQLVEIAHRKFHKLPPGLEPGLQAKFVWEVPTGGALPTPEG
                     RIQIYPCYAFEAHVVLVEIDPDTGKPAIRKYLCGHDCGVMINPDIVHGMTYGGIAHGI
                     GAALYEKFAYTEDGQLASGTFMDYLIPSAMEVPPMAIVDHCTPSPLTTFGQKGSGEAG
                     YLGAPAAIANAINDALEPLGTSIDTLPMSHLAIWKAIGGAGKKAA"
BASE COUNT          357 a          674 c          690 g          395 t
ORIGIN      
        1 gcaagagtgc ctgagcctaa ccatcgggcg tacttgttaa tgatctcaag tctagacata
       61 gaggtgcttg attcaccaat catgagttgg cgattatcgc atcaagctgt cccggatcac
      121 ccggcctgat tcgcgtcagc gcgggcacca gcaatccggc cgcacgcgaa acacgctttg
      181 ggcagtatcg ccaagaattc ccttcctctg cgcctcgttg aaggtggcgc cttcgacgat
      241 cttcttcggt tcgggatcgc cgatcgggaa tggctgatcc gagccgagca tgatgcgcgc
      301 ggagcccgct ttcttcgcca ggtattcgag cgagtccgca tcgaacacgc aggaatcgaa
      361 gtacatcgcc tcgaagccct tgcgcgggtc ggcgtacttg ccctgcgaat tggcgtggtt
      421 gcgcgcgagg cgcccgagcg catagggcaa agcagctccg ccgtgcgaca gcacgaattt
      481 catgcccgga aatttgagga gatgcccgga gaagagcagc cgcgacacag cgatcgaggt
      541 gtcggcgagc ctgccgatcg cgttcaccag atcgtagtcc gcaagccgcg gctcgccgca
      601 caggaacatc gggtgcatga acaccgccgc tttcaggtcc gacgcggtct gccagaaggg
      661 atcgagcgcg gggtcgtcga gatttccggc gttgcctttc ggcagagtac cgatcatcac
      721 tccgccgaaa cccttgtcga gcgcttcgcc cagcacctcc gcggcgagcc cgccgtgctg
      781 cagcggcacc gtcgcgagcg gagtgaagcg cttttcctcg cgcagctcct ccttgaggca
      841 gtcgttgtag aagcggctcc aggcgaggcc ctcgcccgag ggcagctcgt agccgaaact
      901 gtcgagccat ccgccgacga gctgatggtc gatgccgttc ctgtccatcc aggcgcgccg
      961 gtcggcggta tcggagagct tgggcgagat cggccgtgtc ggctccccgc ccggaaactg
     1021 catgcgggtt cccttggcgt cgcgcagcag cctgaccccg ggaaatttcg acgcgttcgc
     1081 gtcgtagcgc ccgtagagca gcttcgggat gaagtgcgcg tggatatcga tgacgaggtt
     1141 tttcacggtc aggctgcttt cttgcccgcg ccgccgatcg ccttccagat cgccagatgc
     1201 gacatcggca gggtgtcgat cgaagtgccc agcggttcga gcgcgtcgtt gatcgcgttt
     1261 gcgatcgccg cgggcgcgcc gagataaccg gcctcgcccg agcccttctg gccgaaagtg
     1321 gtgagaggcg aaggcgtgca gtggtcgacg atggccatcg gcggcacttc catcgcgctc
     1381 gggatcaggt aatccatgaa ggtgccgctc gcgagctgcc cgtcctcggt gtacgcgaac
     1441 ttctcgtaca gtgcggcgcc gataccgtgg gcgatccctc cgtaagtcat gccgtggacg
     1501 atgtccggat tgatcatcac gccgcagtcg tggccgcaca agtatttccg gatcgccggc
     1561 ttgccggtat ccggatcgat ctcgaccaga acgacgtgcg cctcgaaggc gtaacagggg
     1621 tagatctgta tgcggccctc gggcgtcggc agcgcgccgc cggtcgggac ttcccagacg
     1681 aacttcgcct gcagccccgg ctccaggccg ggcggaagtt tgtggaattt ccggtgcgcg
     1741 atctcgacga gctggtccca ggcgagtttt ttctccggcg cgcccttcag gctcacgctg
     1801 ccgtcccggt agtcgaggtc ctcgggcgag cactcgaagt tgtgcgcggc gatggcgatc
     1861 agggtttcct tgatttgctt cgccgcgccc gccgccgcgc cgccgagcat gatcgccatg
     1921 cggctgccga ccgggctgtt ggagggaagc gcggtgaggg aatcggcgcg caccacgcgg
     1981 atcgacgccg gatcgcgctc gaggatttct cccagcacgg tcgagacgag cgactcgtgc
     2041 ccctgccccg cgctcgaatt gttgatgatg cccgtgatcg agcccgagag atcgacgcgc
     2101 accaggcacg agtcca
//
LOCUS       DASPZD010000006         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1563125, whole genome shotgun sequence.
ACCESSION   DASPZD010000006 DASPZD010000000
VERSION     DASPZD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563125"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VEM33_00090"
     CDS             complement(<1..388)
                     /locus_tag="VEM33_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYS50261.1"
                     /translation="MVALSVVIPVYNEEAVLPALFARLYPALDALGVSYEVIFVNDGS
                     ADRSASLLRGQFELRPDATRVILFNGNFGQHLAIMAGFEASRGERVVTLDSDLQNPPE
                     EIAKLLAKMDEGYDYVGSVRRARQDTA"
     gene            complement(390..1520)
                     /locus_tag="VEM33_00095"
     CDS             complement(390..1520)
                     /locus_tag="VEM33_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HYS50262.1"
                     /translation="MNFLPFAQPTIDEAMIAAVADTLRSRWIATGPRVAAFEKALSER
                     FGGRPVRALTSATAAMRVALELLGIGPGDEVIIPAQSFFATGNVVERAGATSVFVDVS
                     LASRNIDLAQAAAAVTPKTRLLLPTHYNAPLDPDGLDAFARRHKVRILEDAALAMGSR
                     TPRGPVGATGDIVSFSFHPNKNMTTIEGGALALNDEREAAIVDELRFHGIKRLPDGTR
                     DVERAGVKYNFSDVSARLGLEQLAHLDEWCRARQRLARHYFECLAGDDLLTPERLPPR
                     DSPGHSWNMFTVLLPLQALRSSRKAFMDAMHKEGIGTGVSYEAIHLTTLFRNKGFREG
                     MFPASERIARETVTLPLFPEMRETDVERVCESMKRVMRRKAA"
     gene            complement(1520..1891)
                     /locus_tag="VEM33_00100"
     CDS             complement(1520..1891)
                     /locus_tag="VEM33_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HYS50263.1"
                     /translation="MNAASWVFLLTGVLLNAAAQLLLKAGTNAVGHFEFSAQNIVPVG
                     MKLAFEPHIAGGLACYVVSVAAWIVGLSRVEVSIAYPMLSIGYVVNALAAWYLFGESL
                     TAQKFIGIAFIVAGVFLVARS"
     gene            complement(1888..>2303)
                     /locus_tag="VEM33_00105"
     CDS             complement(1888..>2303)
                     /locus_tag="VEM33_00105"
                     /inference="COORDINATES: protein motif:HMM:NF037277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50264.1"
                     /translation="SAVIALAFGSLVSVQIATSGHEELSPAYSAYHIVQKIRNQLRPD
                     VPFYVVNTFDHTLLFYLSRTVTMVVNKDELAQPIAWEPRNYLPDTAAFARAWQSDREA
                     FAMFNADDLDAFLKSHPVPMQIVARDARRVIVKKP"
BASE COUNT          396 a          751 c          792 g          364 t
ORIGIN      
        1 acgcagtgtc ctgtcgcgcg cggcgcaccg agccgacgta atcgtagccc tcgtccatct
       61 tggcgaggag ctttgcgatt tcctccgggg ggttctgcag atccgagtcc agcgtgacca
      121 cgcgctcgcc gcggctcgcc tcgaaccccg ccatgatggc gagatgctgg ccgaagttgc
      181 cgttgaacag gataacgcgg gtcgcatcgg ggcgaagctc gaactgcccg cgcagcagcg
      241 acgcagaccg gtcggcgctg ccgtcgttga cgaaaatgac ctcgtagctc accccgagcg
      301 cgtccagcgc cggatacagc cgcgcgaaca gcgccggcag gacggcttcc tcgttgtaga
      361 cgggaatgac gacggagagc gcgaccatgt caagccgcct tgcgccgcat cacgcgcttc
      421 atgctctcgc acacgcgctc gacgtccgtc tcgcgcatct cgggaaagag cggcagcgtg
      481 accgtctcgc gcgcgatgcg ctccgaggcc ggaaacatgc cctcgcggaa tcccttgttg
      541 cggaagagcg tggtgaggtg gatcgcttca tacgagacgc cggtgccgat gccttccttg
      601 tgcatcgcgt ccatgaacgc cttgcggctg ctgcgcagcg cctgcagcgg cagcagcacc
      661 gtgaacatgt tccagctgtg cccggggctg tcgcgcggcg gcaggcgctc gggcgtgagc
      721 aggtcgtcgc ccgcgaggca ctcgaagtag tggcgcgcga ggcgctggcg cgcacggcac
      781 cattcgtcca ggtgcgcgag ctgctccagg cccagccgcg ccgacacgtc ggagaagttg
      841 tacttgaccc ccgcgcgctc cacgtcgcgc gtgccgtcgg gcaggcgctt gatgccgtgg
      901 aagcgcagtt cgtccacgat cgccgcctcg cgctcgtcgt tgagcgccag ggccccgccc
      961 tcgatggtgg tcatgttctt gttgggatgg aagctgaacg acacgatgtc gcccgtcgcc
     1021 cccaccgggc cgcgcggggt ccgcgatccc atcgcgagcg ccgcgtcctc gaggatccgc
     1081 accttgtggc gcctggcgaa cgcgtcgagc ccatcgggat cgagcggcgc gttgtaatgc
     1141 gtgggaagca gcaggcgcgt cttcggcgtg accgcggccg cggcctgggc aagatcgatg
     1201 ttgcgcgagg cgaggctcac gtcgacgaat acggaggtcg cgccggcgcg ctcgaccacg
     1261 ttgccggtcg cgaagaaact ctgcgcgggg atgatcacct cgtcgcccgg cccgatgccg
     1321 agcagctcga gggccacccg catcgccgca gtcgcggaag tgagcgcgcg aaccggccgc
     1381 ccgccgaagc gctccgacag cgccttttcg aacgcggcga ctcggggccc ggtggcgatc
     1441 cagcggctgc gcagcgtgtc ggcgactgcg gcgatcatgg cttcgtcgat ggtcggctgc
     1501 gcgaagggaa ggaagttcac taggagcgcg cgaccaggaa gaccccggcg acgataaagg
     1561 cgatgccaat gaatttctgc gccgtgagcg attcgccgaa caggtaccag gcagcgagcg
     1621 cattcacgac gtacccgatc gaaagcatcg gataagcgat cgaaacctcg acgcgcgaca
     1681 ggcccacgat ccaggccgca acgctcacca cgtagcacgc gagcccgccc gcgatgtgcg
     1741 gctcgaaggc gagcttcatg ccgacgggga cgatgttctg cgcgctgaac tcgaaatggc
     1801 ccacggcgtt ggtgcccgcc ttgagcagca gctgcgccgc cgcgttgagc agcacgcccg
     1861 tgaggaggaa aacccagctc gccgcgttca cggctttttc acgatgacgc ggcgagcatc
     1921 gcgcgcgacg atttgcatgg ggaccgggtg cgacttgagg aacgcgtcga ggtcgtcggc
     1981 attgaacatc gcgaacgctt cgcggtcgga ttgccaggcg cgcgcgaacg cggcggtgtc
     2041 cggcaagtag ttccgcggct cccacgctat cggctgcgcc agctcgtcct tgttgacgac
     2101 catcgtcacg gttcggctca ggtagaacag caaagtgtga tcgaaggtgt tcaccacgta
     2161 gaaagggaca tccggtcgga gctggttccg gattttctgg acgatatgat acgcggaata
     2221 ggccggtgag agctcctcgt gccccgaggt cgcgatttgg acggaaacga ggctgccgaa
     2281 cgcaagggcg atgaccgcgg act
//
LOCUS       DASPZD010000007         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_234814, whole genome shotgun sequence.
ACCESSION   DASPZD010000007 DASPZD010000000
VERSION     DASPZD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234814"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1111
                     /locus_tag="VEM33_00110"
     CDS             185..1111
                     /locus_tag="VEM33_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYS50265.1"
                     /translation="MSYRLLSFKSGAEPRTGLLVDGKVYDAARVTGEPAWSSVLGALR
                     DWAKADRAFAAFAKRIAKTKAKGVPLARVKLLAPVLYPGNIYCAGANYTDHMAEMARA
                     QGKEPGPTMKDLGEKPWHFVKTSRSSVVGPGTAVKLPAYSQKVDWEVELAAVIGRTAK
                     DVPVEKALDCVAGYTIANDLSARDAMRRDKNSPTSPFHYDWVSQKCFDGSCPLGPWIV
                     PAGDIRDPHQLGIKLWVNGELMQDSHTSKLIFDTAEQIAMLSSRVRLHPGDLVLTGTP
                     AGVGMGRGRFLKPGDAVKLWIEEIGELNHTLA"
     gene            1146..1532
                     /locus_tag="VEM33_00115"
     CDS             1146..1532
                     /locus_tag="VEM33_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYS50266.1"
                     /translation="MSKELWDKGLAVRKEVLGAEYVERNLASADDFNMPMQELVTQSC
                     WGWLWARPQMPRKLRSLVNLGMLSALNRPHEFKAHMKGALTNGCTREEIREVLLQVAV
                     YCGMPAGVEAFRLAREAMKEYEEKRS"
     gene            complement(1621..2112)
                     /locus_tag="VEM33_00120"
     CDS             complement(1621..2112)
                     /locus_tag="VEM33_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50267.1"
                     /translation="MNATSSRALLLLIPALLSGCASFSAINPGASPQQVETLVGKPAN
                     VWKNADGSEVWEYPQGPLGVETYMVTLGPDRAVREVRQVLSEEYISKLHVGMPRDEVR
                     RLLGRPRDAGFSDLNDEEIWSWRYREWGVRNMELYAQFDRATGALKKITRFQVDTGDG
                     KRK"
     gene            complement(2128..2613)
                     /locus_tag="VEM33_00125"
     CDS             complement(2128..2613)
                     /locus_tag="VEM33_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50268.1"
                     /translation="MPKAGSSLLLLVLAASLAGCANFTAVTSGMPAQQVQAKLGAPET
                     VRKNADGSEVWEYPEGPYGRQTYMVTVGADRTVREVHQVLNDEYFSRVRAGMSRDEVR
                     RLLGRPGEIMVFGARDEEVWSWRYQQQNAMFFNVLFDRAAGTVRTTQRLEEILLMDRD
                     C"
     gene            2734..>3158
                     /locus_tag="VEM33_00130"
     CDS             2734..>3158
                     /locus_tag="VEM33_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HYS50269.1"
                     /translation="MQRKIPCVLMRGGTSRGPYFLASDLPADPGRRDAVLLSVMGSPH
                     SLQVDGIGGSNTLTSKVAIVSRSQREGADVDYLFAQVSVTEALVDTKPNCGNMLAGVG
                     PFAIEAGLVRPQNPTTTVRIYNVNTQTLIEAVIQTPGGQV"
BASE COUNT          576 a         1029 c         1017 g          536 t
ORIGIN      
        1 atcctttcgc agcttggggt gcactggctg gtgaagcccg aggtattcat cggcatggcg
       61 gcgagcaagt tcgccgacgg aaagctgacc gatgagacga cgcgcaaatt cctcaccgac
      121 cagatggtcg cgttcgagga ctggatcaag cgcgtcaagc gcctcgtcgc gtgaggggaa
      181 ggccatgagc tacaggttgc tgagcttcaa atcaggcgcg gaaccacgca ccgggctgct
      241 ggtcgacgga aaagtctacg acgccgcgcg cgtgaccgga gagcccgcct ggtcctcggt
      301 cctcggcgcg ctccgggatt gggcaaaggc cgatcgcgcg ttcgccgctt tcgccaagcg
      361 catcgccaag accaaggcga agggtgtgcc gctcgcgcgc gtgaagctcc tcgcgccggt
      421 tctctacccc ggaaatatct actgcgctgg cgcgaactac accgatcaca tggccgagat
      481 ggcgcgcgcg cagggcaagg agccggggcc gacgatgaag gacctgggcg agaagccctg
      541 gcacttcgtc aagacctccc ggagctcggt ggtcggcccc ggcacggcgg tcaaactgcc
      601 cgcgtactcg cagaaggtgg actgggaagt cgagctcgcc gccgtgatcg gcaggaccgc
      661 gaaagacgtg ccggtggaga aggcgctgga ctgcgtcgcc ggctacacca tcgcgaacga
      721 tctctcggcg cgcgacgcga tgcggcgcga caagaactcg ccgacctcac cctttcatta
      781 cgactgggtc agccagaagt gcttcgacgg ctcctgcccg ctcgggccat ggatcgttcc
      841 ggcgggcgac atccgcgatc cccatcaact gggcatcaag ctgtgggtga acggcgagct
      901 gatgcaggac tcccacacga gcaagctgat cttcgacacc gccgagcaga tcgcgatgct
      961 gtcttcgcgc gtgaggctcc acccgggcga cctcgtgctc acggggaccc ctgcgggcgt
     1021 gggcatggga cggggccggt ttctgaagcc cggcgacgcg gtcaagctgt ggatcgagga
     1081 gatcggcgag ctgaaccata cgctcgcttg atccttgccg cgcaaccaac cgaaaggaac
     1141 cagcaatgtc gaaagagctg tgggacaagg gactcgccgt gcgcaaggaa gtgctcggag
     1201 ccgagtacgt cgagcgcaac ctcgccagtg cggacgattt caacatgccg atgcaggagc
     1261 tggtgaccca gtcctgctgg ggctggcttt gggcgcggcc gcaaatgccg cgcaagctcc
     1321 gtagcctcgt caatctcggc atgctctccg cgctgaaccg gccgcacgag ttcaaggcgc
     1381 acatgaaggg cgcgctgacc aacggctgca cgcgcgaaga gatccgcgag gtcctgcttc
     1441 aggtggccgt gtactgcgga atgcccgcgg gggtcgaggc gttcaggctc gcccgcgagg
     1501 cgatgaagga atacgaggag aaacgttcct gattcaacct tggctcagtg tttggctcga
     1561 cccccgtcga ggcgagcaga gcggtggcgc ggtggagcgc tgcaatcatg acgcgaaact
     1621 ctatttgcgc ttgccgtcgc cggtatcgac ctggaaccgg gtaatcttct tgagcgcgcc
     1681 ggtagccctg tcgaactggg cgtaaagctc catgttgcgc acgccccact cgcgatagcg
     1741 ccaggaccat atctcttcgt cgttcaagtc ggaaaagccg gcgtccctcg gcctgccgag
     1801 cagccggcgc acttcgtcgc gaggcatccc gacgtgcagc ttggagatgt attcctcgct
     1861 cagcacctgc cggacttccc gcacggcgcg gtcgggcccc agcgtcacca tgtaggtctc
     1921 gaccccgagc ggtccctgcg ggtattccca gacttcggag ccgtccgcgt tcttccaaac
     1981 gttcgcgggc ttgcccacaa gcgtttcgac ctgctgcggg gaagcgcccg gattgatcgc
     2041 ggagaaactc gcgcacccgg acaatagcgc ggggatcagc agcaggagcg cgcgtgaaga
     2101 cgttgcgttc atgactcccc catcgcttca gcagtcgcgg tccatgagga ggatttcctc
     2161 gagccgctgg gtggtcctga ccgtccccgc ggcccggtcg aagagcacgt tgaagaacat
     2221 cgcgttctgc tgctggtagc gccaggacca cacctcttcg tcgcgcgcac cgaacaccat
     2281 gatttctccg ggcctgccga gcaggcgccg gacctcgtcg cgggacattc cggcgcgcac
     2341 cctcgagaaa tattcgtcgt tcagcacttg gtggacttcc cgcaccgtgc ggtcggcccc
     2401 cacagtcacc atgtaagtct gccttccata cggcccctcc ggatattccc acacctccga
     2461 tccgtcggcg ttcttcctca ccgtctccgg cgcgccgagc ttcgcctgca cctgctgcgc
     2521 gggcatcccg ctcgtgacgg cggtgaaatt tgcgcatccg gcaagggacg ccgcgagcac
     2581 gaggagaaga aggctggagc cggcttttgg catgatatcg atccaatgag gtacgatcac
     2641 agactcgaac gaagcctcga cacgtaaaca tcgcaatgtt agcccctatt cctcggttcg
     2701 gcgcgcggca tacaagctga ctggcacctg aacatgcagc gcaagatacc ttgtgtgctg
     2761 atgcgcggcg ggacttcgcg gggcccttac ttcctcgcct cggacctgcc ggcggatccc
     2821 gggcgtcgcg acgccgtcct gctcagcgtc atgggatcgc cgcattcgct gcaggtcgac
     2881 ggcatcgggg ggtcgaacac cttgacgagc aaggtcgcga tcgtcagccg gtcgcagcgc
     2941 gagggcgccg acgtggatta cctgttcgcg caggtctcgg tgaccgaggc gctcgtcgat
     3001 accaagccca actgcggcaa catgctcgcg ggcgtcggcc cgttcgcgat cgaggcgggc
     3061 ctggtccgcc ctcagaatcc caccacgacg gtgcgcatct acaacgtcaa cacgcagacc
     3121 ctgatcgaag cggtgatcca gactcccgga gggcaggt
//
LOCUS       DASPZD010000008         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1443831, whole genome shotgun sequence.
ACCESSION   DASPZD010000008 DASPZD010000000
VERSION     DASPZD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443831"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VEM33_00135"
     CDS             complement(<1..221)
                     /locus_tag="VEM33_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYS50270.1"
                     /translation="MSRSTKIVATLGPASSDRQTLERMFLAGVDVVRMNFSHGTAEDH
                     ERRAELVREACRKTGRTVGIMGDLQGPKIR"
     gene            506..1627
                     /locus_tag="VEM33_00140"
     CDS             506..1627
                     /locus_tag="VEM33_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HYS50271.1"
                     /translation="MFATQIRQKDGIFYFGSVRAPELLAKVRFISRFYGEGEEIAPAR
                     ISHEDEIAQFIAKIERNDRAFQRTLSRAKVRQLKNFYETAITQPPIPGTVLLFTPEKL
                     RFQAGGTDGVGQLAEPQSKFLIIDGQHRLAALRFYLEERPDDAKTINVPCVIFDGRSE
                     DFATEMFVIINSTPTRINKSHLIDLYERVSWAEPHRKFASRLVGSLYSEGDSPLRYRI
                     NRLGGRSAQDKWILQAELFNELHRWVNRDWRKIQLAGGSRTQVPNYYGMVRDFFKAAE
                     RVWGEAWGDGNYMVTKPVTLKAMLRVCADLAREDGGPAEGRVKRWEERLAPWRDLVKA
                     FKSEGFYERFTAKGEVERVGRVHRELARAAGIDARGAKS"
     gene            complement(1624..>2625)
                     /locus_tag="VEM33_00145"
     CDS             complement(1624..>2625)
                     /locus_tag="VEM33_00145"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYS50272.1"
                     /translation="LGRPTEGEFSEKGSLAPIARHLSGLLGEDVPLRRDWLEGVSVEP
                     GRAVMLENCRFNKGEKKDDDALARRMAALCDVYVNDAFGTAHRAEASTHGVAKYAPVA
                     CAGPLMAAEIEALSRALERPARPLVAIVAGAKVSTKLAVLGALAEKVDRLIPGGGIAN
                     TFMLAAGMKVGKSLAESELVGEAKKIAASLRDRGSEVPLPVDVVCAKEVSVTAKAVIK
                     PAGEIGDDDMILDIGPRTAAAFAKRLSEAGTIVWNGPLGVYEFDQFGQGTKAIAEAIA
                     GSGAYSLAGGGETLAAIAKYGVATGISYISTGGGAFLEFLEGKKLPAIEVLEQRGRP"
BASE COUNT          475 a          873 c          792 g          485 t
ORIGIN      
        1 cggatcttgg gaccctgcag gtcgcccatg atgccgaccg tgcgtccggt cttgcggcaa
       61 gcctcgcgca ccaactcggc gcggcgctcg tgatcctcgg ccgtcccgtg cgagaaattc
      121 atgcgcacca cgtccactcc agcgagaaac atgcgctcga gcgtttgccg atcgctggaa
      181 gcgggaccga gagtggcgac gattttggtg cttctgctca attgacgctc cgggtaaacc
      241 gttctacttt accagatgct tatgaggcta ctttccttcc aggaaggcgt tgcgcacgga
      301 actttacggg ctacggcccg ctttgggccc cgctcgctgg agcgtccatc ggccactcga
      361 cgcaaggaga ccgcaaggag cggccccggc ccgacctagg gcaggaccag gtcgctgaac
      421 ccgttttttt cgcagtgggc gcccggtttc ggctatattt cctgtctgcg ccagcggctg
      481 agccgtactt acaagggagg accccgtgtt tgccacgcag attcgccaga aggatggcat
      541 cttctacttc ggcagcgtcc gcgctcccga gctgctcgcc aaggtccgct tcatcagccg
      601 tttttacggt gaaggcgagg agatcgcccc ggcgcgcatc tctcacgaag acgagatcgc
      661 ccagttcatc gccaagatcg agcgcaatga tcgcgccttc cagagaaccc tttcccgggc
      721 caaggtgcgc cagctcaaga acttctatga gacggcgatc acccagccgc ctatcccggg
      781 aacggtgctg ttgtttaccc cggaaaaact gcgcttccaa gcgggtggca ccgacggggt
      841 cgggcagctg gcagagcctc agtccaagtt cctcatcatc gacggccaac accggcttgc
      901 ggcactccgc ttctatttgg aggagcgccc ggacgacgcg aaaaccatca atgtaccgtg
      961 cgtgattttc gacggaagga gcgaggactt cgccaccgaa atgttcgtaa tcatcaattc
     1021 cacaccaaca cgcatcaaca agagccacct gatcgacttg tacgagcggg tatcgtgggc
     1081 agagccgcat cgcaaattcg cctcacgcct agtcggcagc ctctacagcg agggggacag
     1141 cccattgcgc taccgcatca accgcctcgg cgggcgcagc gcacaggata agtggatcct
     1201 gcaggcggaa ctgttcaacg agctgcaccg ctgggtcaac cgcgactggc gcaagatcca
     1261 gctagccgga ggcagccgta cccaggtgcc gaactactat ggcatggtgc gcgacttttt
     1321 caaggcggcc gagcgcgtct ggggcgaggc ctggggcgac ggcaactaca tggtcaccaa
     1381 gccggtgact ctaaaggcca tgctgcgggt atgcgccgac ctcgctcgag aagacggcgg
     1441 gcccgcggaa ggccgagtca agcgctggga agagcgcctc gccccctggc gcgatctggt
     1501 gaaggcgttc aagtcggagg gcttctacga gcgcttcacc gccaaaggcg aggtcgagcg
     1561 tgtgggccgc gtccatcgcg agcttgcgcg cgccgccggg atcgacgcac gcggggccaa
     1621 gtcctagggt cggccgcgct gctccagtac ttcgatcgcg ggcagtttct tgccttcgag
     1681 gaactccagg aaagctccgc cgccggtcga gatgtaggaa atccctgtcg cgactccgta
     1741 tttcgcgatc gcggcgagcg tctcgccacc gccggcgagc gaataagcac ccgatccggc
     1801 gattgcttcg gcgatcgcct tcgttccctg tccgaattgg tcgaattcgt acacgccgag
     1861 cggaccgttc cagacgattg tgcccgcctc ggagagccgt tttgcgaacg ccgcagcggt
     1921 acgcgggccg atatcgagaa tcatgtcgtc gtcgccaatc tcgcccgcag gcttgatcac
     1981 tgccttggcc gttaccgaga cttctttcgc gcagacgacg tccacgggca acggtacctc
     2041 gctgcctcgg tcgcgcaggc ttgctgcgat tttcttcgcc tcgccgacga gctcggactc
     2101 ggccaaagac ttgcctactt tcatgccggc cgcgagcatg aaggtattgg caatacctcc
     2161 acccgggatc agtcggtcga ccttttctgc gagggcgccc aggaccgcaa gcttggttga
     2221 gactttcgcc ccggcgacga tcgccaccag cggacgtgcc gggcgctcaa gcgccctaga
     2281 caatgcctcg atctccgccg ccatcagcgg cccggcgcag gccaccggcg cgtacttcgc
     2341 aacgccgtga gtgctcgctt cggcgcggtg cgcggtgccg aaggcgtcgt tgacgtatac
     2401 gtcgcacaag gccgccatcc gtctcgccag cgcatcgtcg tcctttttct cccccttgtt
     2461 gaaccgacag ttctccaaca tcaccgccct gccgggttcg actgaaaccc cttccagcca
     2521 gtcacgcctg agcggcacgt cctcgccgag caatccggac aagtgtcttg cgatcggggc
     2581 gagggacccc ttctcgctga actcgccctc ggtcggccga ccgag
//
LOCUS       DASPZD010000009         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1799810, whole genome shotgun sequence.
ACCESSION   DASPZD010000009 DASPZD010000000
VERSION     DASPZD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799810"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VEM33_00150"
     CDS             complement(<1..1162)
                     /locus_tag="VEM33_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYS50273.1"
                     /translation="MSKALTRLSAVELARLVRTKKASPVEVVEAHLAAIQRINPAVNA
                     FCTVAAEKALTWAREAERAVKRRAKLKPLHGVPVAIKDLTPTADIRTTFGSTLFRDHV
                     PTEDAEVVRRLKAAGAIVLGKTNTPEFGAGANTVNKVFGATRNPWNTALSASGSTGGG
                     AAALAARMAPLAEGTDFGGSLRTPAAFCGVLGLRTTAGLVPKHPAAMPWHDQSVAGPM
                     ARSAEDCALLLDAMTGLSGKSPLSAPAPWRSAREAVAKARSLRGLRLAYAPDIAGIGV
                     DEEIERICRTAARDLRDADGAALEEIEFSLSDGRDAFLALRGEAMLGNHFDRLERIGE
                     LGDNLAGNIREGLALTISEIARAERKRAEIWHRWRALFEQYDALLTPTAPVPP"
     gene            1271..>2254
                     /locus_tag="VEM33_00155"
     CDS             1271..>2254
                     /locus_tag="VEM33_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50274.1"
                     /translation="MSQPNQYELLKQRRFLPFFLTQFLGAFNDNVYKNALVILVAFHA
                     ASLSKLDANTLSNLAQALFILPFFLFSATAGQIADKLEKSRLIRWVKLAEIAIMTLGA
                     MGLYWRSLSLLMTALFLMGLHSTVFGPVKYAYLPQHLGQEEIVGGNGLVEMGTFLAIL
                     VGTLLGGYLIAEGSATPVAVTVMALAALGYLTSRWVPRSPAAAPDLSINWNPLSETWR
                     ILQFMRGNRTVFLSVLGISWFWFLGALYLSQFPVYAKDVLFGTEQVVTVLLALFSVGV
                     GIGSLLCERLSGRRIEIGLVPFGSIGLTLFGIDFYFASAGLPAHAAVGAADF"
BASE COUNT          312 a          772 c          753 g          417 t
ORIGIN      
        1 acggcggaac cggcgcggtc ggcgtgagga gcgcgtcgta ctgctcgaac agcgcgcgcc
       61 agcggtgcca gatctccgcg cgcttgcgct ccgcgcgcgc gatctcgctg atggtgagcg
      121 cgagcccttc gcggatgttg ccggcgaggt tgtccccgag ctcgccgatg cgctccagcc
      181 ggtcgaaatg attgcccagc atcgcctcgc cgcgcagcgc gagaaaggcg tcgcgcccgt
      241 ccgacaggct gaattcgatt tcctcgagcg ccgcgccgtc ggcgtcgcgc aggtcgcgcg
      301 ccgcggtgcg gcagatgcgc tcgatctcct cgtccacgcc gattccggcg atgtcgggcg
      361 cgtaggcgag gcgcaggccc ctcaggctcc tcgccttcgc gaccgcctcg cgcgcgctcc
      421 tccacggcgc gggagcggag agcggcgact tcccggaaag cccggtcatg gcatcgagaa
      481 ggagggcgca gtcctcggcc gagcgcgcca tcggcccggc cacgctctgg tcgtgccagg
      541 gcatcgccgc cgggtgcttg ggaacgagtc ccgcggtggt gcgcagtccg agcaccccgc
      601 agaaggccgc cggcgtgcgc agcgacccgc cgaaatcggt gccctcggcg agcggcgcca
      661 tgcgcgcggc gagcgccgcc gccccgcccc cggtcgagcc cgaggccgaa agcgccgtgt
      721 tccaggggtt gcgcgtcgcg ccgaacacct tgttcaccgt gttcgcgccc gcgccgaact
      781 caggcgtgtt ggtcttgccg agaacgatcg cgcccgccgc cttgagcctg cgcaccacct
      841 ccgcgtcctc ggtgggcaca tgatcgcgga acagcgtcga gccgaaggtc gtgcggatgt
      901 ccgcggtggg cgtgaggtcc ttgatcgcca ccgggacgcc gtgcaggggc ttcaacttgg
      961 cgcgcctctt caccgcccgc tccgcctcgc gcgcccaggt gagcgccttc tcggcggcga
     1021 cggtgcagaa ggcgttgacc gcggggttga tcctctggat cgccgcgaga tgcgcctcga
     1081 ccacttcgac cgggctcgcc tttttcgtgc gcacgagccg cgcgagctcg accgctgaaa
     1141 gtcgggtgag tgccttggac atgggaaggg ttcgcgcaat cgcggtgcgt gcgggtgccg
     1201 cacatcatat actttcccgc gccggcaaac gccgcagccg gcggcaccgc gagcggagga
     1261 tggtggaccc atgagccagc ccaatcagta cgaattgctc aagcaacggc gctttctccc
     1321 gtttttcctg acacagttcc tcggggcctt caacgacaac gtctacaaga acgccctggt
     1381 cattctcgtc gcctttcatg cggcaagcct gagcaagctc gacgcgaata ctctttcgaa
     1441 cctggctcag gccctattca tcctgccgtt ttttctcttc tccgcgaccg ccgggcagat
     1501 cgccgacaag cttgagaaat cgcgtctgat tcgctgggta aagctcgcgg agatcgcgat
     1561 catgacgctc ggcgccatgg ggctgtactg gcgcagcctc tccctgctga tgaccgccct
     1621 cttcctgatg ggcttgcatt cgacggtgtt cgggccggtg aaatacgcgt acctgcccca
     1681 gcacctcggg caggaggaga tcgtcggggg gaacggcctg gtggagatgg gcaccttcct
     1741 cgccattctc gtcggcacgc tgctcggcgg atatctgatc gccgaggggt cggcaacacc
     1801 ggtcgccgtg accgtgatgg ccctcgccgc gctcggctat ctgacgagcc ggtgggtgcc
     1861 gcgttcaccc gccgccgcgc cggacctctc gatcaactgg aatccgttgt ccgagacctg
     1921 gcggattctc cagttcatgc gcggcaaccg gacggttttt ctctcggtgc tcgggatttc
     1981 ctggttctgg tttctcggcg cgctgtacct gtcgcaattt cccgtgtacg cgaaggatgt
     2041 ccttttcgga acggaacagg tggtcaccgt gctgctcgcg ctgttctccg tcggggtcgg
     2101 aatcggttct ctcctgtgcg agcggctctc gggccgcagg atcgaaatcg gattggtgcc
     2161 cttcggttcg atcgggctca cgctgttcgg catcgatttc tatttcgcga gcgcgggctt
     2221 gcccgcgcac gcggcggtcg gcgcggcgga tttc
//
LOCUS       DASPZD010000010         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1561216, whole genome shotgun sequence.
ACCESSION   DASPZD010000010 DASPZD010000000
VERSION     DASPZD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561216"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /gene="hemC"
                     /locus_tag="VEM33_00160"
     CDS             <1..775
                     /gene="hemC"
                     /locus_tag="VEM33_00160"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYS50275.1"
                     /translation="RPLAQIGGKGLFVKELEAALEDGRADLAVHSAKDVPMRMPQGFC
                     LAAIPARADPRDCLVSGLSASLEALPPGAIVGTSSLRREAQLHERYPALGVKALRGNL
                     DTRLAKLDRGEFQALVLAAAGLKRLGLGARIRAMLEPEQSLPAPGQGALAIECREDRG
                     DLRQWVAPLDDAAASACVRAERALSRALSGSCQLPLAAYAMSKAGEIRLRGLVAMPDG
                     SKVVRAESSGPIAAPENLGEMLATQLRTLGADAILASLS"
     gene            805..1545
                     /locus_tag="VEM33_00165"
     CDS             805..1545
                     /locus_tag="VEM33_00165"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYS50276.1"
                     /translation="MVTRAREHAPALAERIRAAGGDPIIFPTIEILPPENPGAVRDLI
                     ERLDGFQLAIFVSPTAAMRGHGLVSASRSWPEGLRVAAVGMGTASALEELGFHSVIAP
                     SGEADSEALAALPELRDLQGRSIVIFRGQGGREWLRTRLEARGARVEYAECYRRTRPN
                     ADAGPLLARWQSGGVEAVAIASAEGLENFFDMLGPTGRGYLRATPVFVPHPRIALAAR
                     RLDVREIVVTGRGDDRTVAEIAAFFAKV"
     gene            1571..>2347
                     /locus_tag="VEM33_00170"
     CDS             1571..>2347
                     /locus_tag="VEM33_00170"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYS50277.1"
                     /translation="MENDPQRASKRRAWSISPTTVAFVLVTLLLVWQWYDGQSRIGAL
                     RDELAQRVRESESDSRDARLAARQAQEAAREAQAKLAQIEVKFTEFQNHQIGLEALYQ
                     ELSRSRDEWVLAEIEQVLTIASQQLQLTGNVQVAVAALQTADARLARPGRPQFVPLRK
                     VFARDIERLKTAPSLDVPGLVVKLDQVIAAVDSLPLTQDARPLAAAVARRESDGFWER
                     LVAELLGELKQLVRIQNVEGADPALLSPPQAFFLRENLKLR"
BASE COUNT          377 a          743 c          846 g          381 t
ORIGIN      
        1 ccggccgctc gcccaaatcg gcggcaaggg ccttttcgtc aaggagctcg aggccgcgct
       61 cgaggacggg cgggccgatc ttgccgtgca ctccgcgaag gacgttccga tgcgcatgcc
      121 gcagggattt tgcctggcgg ccattcccgc gagagcggat ccgcgagatt gcctcgtgtc
      181 cggcctgagc gcgagtctgg aagcgctgcc gcccggggcc atcgtcggga cttccagctt
      241 gcggcgcgag gcgcagttgc acgaacgtta tccagcgctg ggggtgaagg cgctgcgcgg
      301 caatctggac acgcgtctgg cgaagctcga tcggggggaa ttccaggcat tggtgctcgc
      361 tgcggcaggc ctcaagcggc tcggccttgg tgcgcgaatt cgcgcgatgc tcgagccgga
      421 acagagcctg cccgcgcccg gacagggggc gcttgcgatc gagtgccggg aggatcgcgg
      481 cgatctgcgc cagtgggtcg ctcccctgga cgatgccgcc gcatccgcgt gcgtgcgggc
      541 cgagcgtgcg ctgagccgcg cgctgtcggg gagctgccaa ctgccactcg ccgcgtacgc
      601 catgtcaaaa gcaggggaga tccgtttgcg gggtctcgtg gcgatgcccg acggcagcaa
      661 ggtagtgcgc gcggagtcga gcggcccgat agcggcgccg gaaaacctgg gtgagatgct
      721 cgcgacgcag ctgcgaactc tgggcgcgga cgcgattctt gcatctttat catgagggcg
      781 ggaccgctct cgggccgcgg tatcgtggtc actcgcgcgc gcgaacacgc tccagcgctt
      841 gccgagcgga tccgcgcagc gggcggagac ccgattatct ttcccaccat cgagatactg
      901 ccgccggaga acccaggcgc ggtccgcgat ctcatcgagc gcctagacgg atttcagctt
      961 gcgattttcg tcagtccgac ggcggcgatg cgggggcacg gactcgtgag cgcgagccgg
     1021 agctggccag aggggctgcg cgtggccgca gtgggaatgg gaaccgcaag cgcgctcgag
     1081 gaactcgggt tccattcggt gattgcgccc tcgggcgagg ccgacagcga ggcgctcgcc
     1141 gcgcttcccg agctgcggga tctgcaaggg cgctcgatcg tgatctttcg cgggcagggc
     1201 ggccgcgaat ggctgcgaac gagactcgaa gcgcggggcg cgcgagtcga gtacgccgaa
     1261 tgctaccgcc gcacgcggcc caacgccgac gcaggaccgc tgctcgcccg ctggcaaagc
     1321 ggcggggtcg aagcggtcgc tatcgccagc gccgaaggct tggagaattt cttcgacatg
     1381 ctgggcccga ccggcagagg atatttgcgc gcgaccccgg tgttcgtgcc gcatccgcgg
     1441 atcgcgcttg ctgcgcgcag gctcgacgtg cgggagatcg tcgtgaccgg gcgcggcgac
     1501 gatcgcaccg tagcggagat cgccgctttt tttgctaaag tatgatcgcc tttcaccggt
     1561 aggttcacgc atggaaaacg atccgcagcg agcttcgaag cgcagggcgt ggagtatttc
     1621 gcccacgacg gtcgcattcg ttctcgtcac cctccttttg gtgtggcagt ggtacgacgg
     1681 tcagagccgg atcggcgcgc tgcgcgacga gcttgctcag cgcgtgcgcg aaagcgagtc
     1741 cgacagccgc gacgcgcgcc tcgccgcgag acaggcacaa gaggcggcgc gtgaagcgca
     1801 ggcgaagctt gcgcagatcg aggtgaagtt cacggaattt cagaatcatc agatcggcct
     1861 cgaggcgctc taccaggagc tgtcgcgcag ccgcgatgaa tgggtcctcg ccgaaatcga
     1921 gcaggtgctc accattgcct cgcaacaact gcagctcacc ggtaacgtcc aggtggccgt
     1981 ggccgctttg cagacggccg atgcgcgcct cgcgcgtccg ggccgcccgc agttcgtgcc
     2041 gttgcgcaag gtcttcgccc gggacatcga gcgcctgaaa accgcgccca gcctggatgt
     2101 tccggggctt gtggtgaaac tggatcaggt gatcgcggcc gtggattccc tgccgctcac
     2161 acaggacgcg cgcccgctgg ccgcggccgt cgccaggcgc gagtcggatg ggttctggga
     2221 aaggctggtc gcggagctgc tcggcgagct caagcagctc gtcaggatac agaacgtgga
     2281 gggggccgac ccggcgctgc tcagcccgcc gcaggcgttt ttcctgcgcg agaacctgaa
     2341 actgcgc
//
LOCUS       DASPZD010000011         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_716022, whole genome shotgun sequence.
ACCESSION   DASPZD010000011 DASPZD010000000
VERSION     DASPZD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716022"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="VEM33_00175"
     CDS             complement(<1..2131)
                     /locus_tag="VEM33_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYS50278.1"
                     /translation="MAEISLKPGQLLTGSLFSEPMRVETVRSHGNDAWVVGLVGTETE
                     RFRSVTLTVQDLKTLKILSPSGSYDGDGKLLRLGLQAYALGIAYEFDPYFGLSISRVD
                     PLPHQLEAVYDYLLKLARVRFLLADDAGAGKTIMSGLLIRELKLRGLAERILVVCPAN
                     LAFQWQRELREKFDEKFVVLRGGDIRDQFGVNQWLEQKLLITSLDLAKRTDVLPGLKQ
                     VHWDLVIVDEAHRMSWTPPSRKTARYALGELLRDTSDHFLMLTATPHKGDPVNFSLFL
                     QLLDPDAYADVKSIDTAMTRRRAPFYLRRTKEAMVYFPERQPDGTWEARKIFTKRIPH
                     TVDFQIDGAEFDLYREVTRFVKQQSARAAADGDNPRARAVGFLMSLYQRRLASSTYAM
                     RHSLENRARRLEEGLKRAQDLAREAPPDLPDPEELEEMEEGERERLERVLEAITLAGN
                     AEQVRQEIVDVRELAEHAKVVEDSGTEAKLSRLKELLDREGFFERPDQRLLLFTEFRD
                     TLDYLVERLKTWGFKVGSIHGGMKAGSRDEPGTRLHAEQQFREGVIQMLVATEAAGEG
                     INLQVCHILFNYDIPWNPNRLEQRMGRIHRYGQTEDCLIFNFVATNTIEGHVLHRLLE
                     KLQEIRDALDDDSVFNVVGEILPAAHVERVLREYYAGNLGDGDLEDRLLRNVDERQFR
                     AICQNALEGLATKKLNLAMLLERRARAQ"
     gene            complement(2197..2421)
                     /locus_tag="VEM33_00180"
     CDS             complement(2197..2421)
                     /locus_tag="VEM33_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYS50279.1"
                     /translation="MAIRVQLRDLLEKRGMAQTELQARSGLGYSTINALYHGKTERVE
                     FATLEALCEVLECGVGQILEYVPEKKRGRP"
     gene            2658..3026
                     /gene="radC"
                     /locus_tag="VEM33_00185"
     CDS             2658..3026
                     /gene="radC"
                     /locus_tag="VEM33_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYS50280.1"
                     /translation="MRGPQDAVNLLRERAARELIEVFYVIALNSQSRVIAIQEVTRGI
                     LNSSLVHPREVFRLAIAFGAAGIIVAHNHPSGDPTPSADDRAITRQLVDAGRLLDIPV
                     YDHLVLGASRYLSFAEAGLL"
     gene            3405..3752
                     /locus_tag="VEM33_00190"
     CDS             3405..3752
                     /locus_tag="VEM33_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50281.1"
                     /translation="MPASRQRTATKLLRLYPEELAHITARAQAYGLTPARFIREAALG
                     PRPKTWHHREADRIIRALARIGRSLEHIARLTRPGLNTAVAKHLADLRAEYDALVRQL
                     MRNGGQGSRRSSP"
     gene            3749..>5034
                     /locus_tag="VEM33_00195"
     CDS             3749..>5034
                     /locus_tag="VEM33_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HYS50282.1"
                     /translation="MIATTSSGRRFAALARYLVYGRSGEETERVAWTAGRNLGTDDPE
                     VAAAMMQATANANEQVEGPVYHLTINFDPADPVTPERMQAVADRVLADLGLAEHQALM
                     VAHRDRAHPHVHVMVNRVHPETGRAWDRWQDQPKIQRTLRMLERELGLREVAGRLYQL
                     DGQEPPERAALTNGELRQAERSGDPAFPDRVRAHLAELRVARSWEELEATLARHGLRL
                     ERKGQGLVITDGKFQIKASRVARDLSLKRLEQRFGVPFPERETPAPAREPLSPAVEQV
                     TKALDEHERLATLTAERGRANQDLAAARERLQRLNGAMERVRRASERFDAALARVYRD
                     PPAARVRFTRTAVEIGAERAVAVLGTEPEQFGALKTVDRRRALGLGVARDDRPARLAA
                     PSAAGRARELAEAHAALGMAARGHGDHPDTQREATG"
BASE COUNT          899 a         1721 c         1546 g          868 t
ORIGIN      
        1 cttgtgcccg agcccggcgc tcgagcaaca tcgccaggtt gagtttcttc gttgccaggc
       61 cctccaacgc gttctggcaa atcgcgcgga actgcctctc gtcgacgttg cggaggagcc
      121 ggtcttcgag gtcaccgtca cccaggttac cggcgtagta ctctcggagc acccgctcta
      181 catgcgccgc agggagaatc tcacccacca cgttgaacac cgagtcatcg tcgagagcgt
      241 cgcggatttc ttggagcttc tcgagcaggc ggtgaagcac gtgaccttcg atcgtgttcg
      301 tggccacgaa attgaagatc aggcaatctt cggtttgccc ataccgatga attcgaccca
      361 tccgctgctc aaggcggttc ggattccagg ggatgtcgta gttgaagagg atatggcaaa
      421 cctgaaggtt gatgccctcc cctgccgctt ccgttgccac gagcatctga atcacgcctt
      481 cacggaactg ctgctcggca tggagccgag tccctggttc gtcacgcgaa ccggccttca
      541 tcccaccatg gatgcttcca accttgaacc cccacgtctt gagacgctcc accaggtaat
      601 cgagcgtgtc cctgaattcg gtgaagagca acagccgctg atcagggcgc tcgaaaaacc
      661 cttcccgatc gagcagctcc ttgagacgcg agagcttcgc ttcagtccca gagtcttcga
      721 ccactttggc atgctccgcc aattcgcgga cgtcgacaat ttcctgacga acctgttcgg
      781 cgttgcccgc gagcgtgatc gcctccagaa cgcgctcaag ccgctcccgt tcgccttctt
      841 ccatctcctc cagttcttcc gggtccggca ggtcgggagg tgcctcacgt gctaggtcct
      901 gggcgcgctt cagaccttcc tccaaccttc ttgctcgatt ctctaaggag tgacgcatgg
      961 cgtacgtgct ggacgctagt ctccgctgat acagggacat taggaagccg accgctcgcg
     1021 cccgaggatt gtccccgtcc gcggcggctc gagcgctctg ttgctttacg aagcgggtga
     1081 cctcccgata caggtcgaat tcagcgccgt caatctggaa atcgacggta tgggggatgc
     1141 gtttggtgaa gattttccgg gcttcccacg tcccgtcggg ctgccgctct gggaagtaga
     1201 ccatggcctc cttggtccgg cgcaggtaga acggcgcccg gcgccgtgtc atagccgtat
     1261 caatagactt cacgtctgcg taagcatcgg gatcaaggag ctgaaggaac agactaaagt
     1321 tgaccggatc gcccttgtgc ggcgtggccg tcagcatcag gaagtggtcg ctcgtgtcac
     1381 gcaacagttc gccgagtgcg taacgcgcgg tcttcctcga cggcggggtc cacgacatgc
     1441 ggtgtgcctc gtccacgatc acgagatccc agtgcacctg tttcaggccg ggcagaacat
     1501 cggtacgctt cgcgaggtcc agggaggtaa tcagcagctt ctgttccagc cactggttca
     1561 cgccaaattg atcgcggatg tcgccgcctc ggagtaccac aaacttctcg tcaaacttct
     1621 cccgaagctc tcgctgccac tgaaacgcga gattggcagg acagacaacc agaatgcgct
     1681 cggcgagccc cctgagcttc agctcccgga tcaacaatcc ggacataatg gtcttgcccg
     1741 ctccagcgtc gtctgccagt aggaagcgga ccctagcgag cttgagaagg tagtcgtaga
     1801 cggcttccag ctggtggggc agcggatcca ccctcgagat ggagagcccg aagtacgggt
     1861 caaactcgta cgcgatcccc agcgcgtagg cttgcaggcc cagccgaagc agctttccgt
     1921 cgccgtcata cgaacccgaa ggcgacagga ttttcagagt cttaaggtcc tggacggtaa
     1981 gggtcacact ccgaaagcgt tcggtctccg tgcccacgag gccgaccacc cacgcgtcgt
     2041 tgccgtgtga ccgaactgtc tcgacccgca tcggctcgct gaagagggag ccggtcaaga
     2101 gctgtccggg ctttagtgag atttcggcca tatcctcaaa tcgctagagc caagactctt
     2161 ggaaagcagc agcaaactaa agcgcaatgc tcggcgtcag gggcggcccc gcttcttttc
     2221 cggaacgtac tccaggatct gtccaacccc acactccaga acctcgcata gcgcctcgag
     2281 cgtcgcaaac tcgacgcgct ccgtcttccc gtggtacaac gcgttgatgg tcgaataacc
     2341 gagcccactc cgcgcttgga gctcggtctg cgccataccg cgcttctcca gcaggtcccg
     2401 tagttgcacc cgaatggcca tagcccgccc tgacgtgggt cggcatgaat atatcggtcc
     2461 tgcaatatat cgccagcact tgacaaagta tatcgctagt gatatattgt tagcgagaca
     2521 tgcaccgcgt cacttgcgag gggatgggag gcaggcaggc catgtatcgc ccacaccgga
     2581 gacgttccga ggccctacgg cccgcctggg ttcgcctgat caaggaagtg gacccgtcgc
     2641 agccggagcc tgtccaggtg cgaggcccgc aagacgcggt caacctcttg cgcgagcgcg
     2701 cggcccgaga actcatcgaa gtgttctacg tgatcgcgct caactcgcag tcccgcgtga
     2761 tcgccatcca ggaagtcacg cgggggatcc tcaattcttc cctggtgcac ccgcgcgaag
     2821 tgttccggct cgccatcgct ttcggcgcgg cgggcatcat cgtggcccac aaccacccga
     2881 gcggggaccc taccccaagc gccgatgacc gcgcaatcac gcggcagctg gtcgacgccg
     2941 ggcgcctctt ggacattccc gtgtatgatc acctcgtcct cggtgcatct cgctatctga
     3001 gcttcgcgga agctgggttg ctctgaggcg gccggcgccg gccagggaaa gcctcaatcg
     3061 gcggccgtcc ggaggcgagc ccgcccgttg tgataaccga atgctgagcc ggttttccac
     3121 acggcgagcg ggattcccgc cctgcaccag ttctcgtagc ggtttctgct gtgcaagcta
     3181 gtgggggaga tatcggtgca cgcgctggtg cgcgtgcccc gatcctcccc caatgggccc
     3241 attcgcgccc tgacgggcgc tcaaaggcca ccacccccac cccagcaccc gtagccgacc
     3301 ccaaacacgc gcccccgacc ccagtcgggc acgctcgggg tcattcgggc ctctttacca
     3361 gtagagggat ctgcgttcta ccgacctgcg acttcggagc gtccgtgccc gccagtcgcc
     3421 aacgcaccgc caccaaactc cttcggctct accccgagga gctcgcgcac atcaccgcgc
     3481 gcgcccaggc ctacggcctc acgcccgcgc gcttcatccg cgaagcggct ctcggaccga
     3541 ggccgaaaac gtggcaccac cgagaggccg accgtatcat ccgcgcgctc gcccgcatcg
     3601 gccgcagcct cgagcacatc gcccggctca cccgcccggg cctcaacacc gcggtagcca
     3661 aacacctggc cgaccttcgc gccgagtacg acgcgctcgt cagacagctc atgcgaaacg
     3721 gcgggcaggg ctcgcgccgg tcgtctccgt gatcgccacg acctcctccg gccgacgctt
     3781 cgcggcgctc gcccgctacc tcgtgtacgg tcgctccggg gaggagaccg agcgcgtcgc
     3841 ctggactgcc ggtcggaacc tcggcaccga cgaccccgaa gtcgctgcgg ccatgatgca
     3901 agctacggcc aatgccaacg agcaggtcga gggccccgtg taccacctga cgatcaactt
     3961 cgaccccgcc gatcccgtca cgccagaacg gatgcaggcc gtcgccgatc gcgtcctcgc
     4021 ggatctcggc ctcgccgaac atcaggcgct gatggtcgcg catcgcgacc gggcgcaccc
     4081 acatgtgcat gtcatggtca accgcgtcca tcccgagacc ggccgggcct gggaccgctg
     4141 gcaagaccag ccgaagattc agcgcaccct gcggatgctc gagcgcgagc tggggctgcg
     4201 ggaggtcgcg ggccggttgt accagctcga tgggcaggag ccgcccgagc gcgcggccct
     4261 cacgaacggc gagcttcgtc aagcagagcg cagcggggat ccggccttcc ccgatcgcgt
     4321 gcgcgcgcac ctcgccgagc ttcgggtggc ccggtcgtgg gaggagctcg aagcgacact
     4381 ggcgcgccac gggctccggc tcgagcgcaa gggccagggg ctcgtgatca cggacgggaa
     4441 attccaaatc aaggcctcgc gcgtggcgcg cgatctctca ctcaagcgcc tcgagcagcg
     4501 ctttggcgtg ccgttccccg agcgggagac gccggcgccc gcacgtgagc ccctgagtcc
     4561 cgcggtggag caggtcacga aggcgctcga cgaacacgag cgactcgcga cgctcacggc
     4621 cgagcgcggg cgtgccaatc aggacctcgc cgccgcgcgt gagcgcctgc agcgcctgaa
     4681 cggcgcgatg gagcgcgtcc ggcgcgcatc tgagcgcttc gatgccgccc tcgcccgcgt
     4741 ctatcgagat ccgccggcgg cccgtgtgcg cttcacgcgc accgcggtgg agatcggcgc
     4801 ggagcgagcg gtcgcggtgc tcgggacgga acccgagcag tttggggcgc tcaagaccgt
     4861 cgatcggcgt cgcgcgttgg gactcggggt cgcccgggac gaccggccag cacgtctcgc
     4921 ggcaccgagc gcggccgggc gcgcccgcga gctggcggag gcgcacgccg cgctgggtat
     4981 ggcggcccgc gggcacggag accatcccga cacgcagcgg gaagcgaccg ggag
//
LOCUS       DASPZD010000012         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_957116, whole genome shotgun sequence.
ACCESSION   DASPZD010000012 DASPZD010000000
VERSION     DASPZD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957116"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VEM33_00200"
     CDS             complement(<1..526)
                     /locus_tag="VEM33_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50283.1"
                     /translation="MIFTEMYADTLKLIVTADRRHPSPRLPERLNGLFRLLGDASGGR
                     AERIEDEIWRLWMAYRDSAAAFDLERATRALTALDFPAAEGILARLVATHPDFAEAWN
                     KRATLYYMQSRDEESVASIHRTLELEPRHYGAICGFAQICLSLGDTDSALFAFDAALR
                     VNPHLESVREALDKL"
     gene            complement(513..>2895)
                     /locus_tag="VEM33_00205"
     CDS             complement(513..>2895)
                     /locus_tag="VEM33_00205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HYS50284.1"
                     /translation="PELLEETVRVAGGCRFSLEELRYEYPEELVPDGETPASWLRKLA
                     EEGLQRRFPVGASQKIRDLMERELSLIAELRYEPFFLTVHDIVRYARSRDILCQGRGS
                     AANSAVCYALGITEVNPAKQELLFERFISKERNEPPDIDVDFEHQRREEVIQYVYAKY
                     GRERAALAATLITYQPKSALRDVGKALGLDALQVDRIAKSIAWWDDRRELATRLRDTG
                     FDPESPVMLRLVGLASALLGFPRHLSQHVGGFVISRGALARLVPIENAAMPERSVIQW
                     DKDDLENLGLLKVDVLALGMLSAIRRALDFVNELRGSSITMDSLLREEDPAVYEMMRH
                     ADTIGVFQIESRAQMSMLPRLKPEHFYDLVIEIAIVRPGPIQGDMVHPYLRRRAGTDP
                     IDYPNEAVRKVLERTCGVPIFQEQVMQLAVVAAGFTPGEADQLRRAMAAWRRKGGLEP
                     FHKKLMDGMRKNGISAEFAERIYRQILGFGEYGFPESHSVSFALLAYVSAWLKHHEPA
                     AFLAALLNSQPMGFYTPSQLVQDARRHGVEVRPVDALASEWDCTLETGTDALAVRLGF
                     LLVQGLSKTGAERIVAARRDRLFGSVEDLARRAELNRHDLKCLAAAGAFEALSGHRRH
                     AFWQVAGIETGAHILRDAPVAETPAILPRPTEGENLVADYASTGLTLGRHPLALLRAH
                     LSRMRFATAGELKLLPGGTRARAAGIVTGRQRPSTASGTVFVTLEDETGYVNVIVWPH
                     LIERQRRELLGARLMGVEGVLEREGEVMHLVASKLSDHSALLGGLVTPSRDFH"
BASE COUNT          504 a          953 c          945 g          493 t
ORIGIN      
        1 gcagcttgtc gagcgcctcg cgcacgctct ccaggtgagg gttgacgcgc agcgccgcgt
       61 cgaaggcaaa gagcgcgctg tcggtatctc cgagcgacag gcagatctgc gcgaaaccgc
      121 agatcgcgcc gtagtggcgc ggctcgagct ccagggtgcg gtggatgctg gccacgcttt
      181 cctcgtcccg gctctgcatg tagtagagcg tggcgcgctt gttccaggct tcggcgaaat
      241 ccgggtgggt ggcgacgagc ctcgcgagga tgccctcggc cgccgggaaa tcgagcgcgg
      301 tgagggcgcg cgtcgcccgc tccaggtcga acgccgcagc ggaatcccgg taggccatcc
      361 acagcctcca gatttcgtcc tcgatgcgtt ctgcgcgtcc gccgctcgca tcgcccagca
      421 agcggaacag gccgttcagg cgctcgggca gccgcggcga agggtgccgc cggtcggcag
      481 tgacgatcaa tttgagcgtg tccgcgtaca tttcagtgaa aatcacgcga cggcgtgacc
      541 aagccgccca gcagggcgct gtgatcgctc agcttgctgg ctacaagatg catcacttca
      601 ccttcgcgtt caagcactcc ttcgacgccc atcagacgcg cgcccagcag ttcgcgccgc
      661 tggcgctcga tgagatgcgg ccagacaatg acattaacat agccggtctc gtcctcgagc
      721 gttacaaata ccgttcccga ggcggtgctc gggcgctggc gaccggtgac gatgcccgcc
      781 gcccgcgccc gagtgccgcc gggaagcagt ttcagttcac cagcggtggc aaagcgcatg
      841 cgcgacaggt gcgcccgcag cagcgcaagc gggtggcgcc ccagagtgag accggtgctc
      901 gcgtagtcgg ccacgaggtt ctctccctcg gttggacgcg gcaaaatcgc cggagtttcc
      961 gcaaccggcg cgtcgcgcaa gatgtgcgcg ccggtttcga ttccggcgac ctgccagaat
     1021 gcgtggcggc gatggccgga gagcgcctcg aacgcgcctg cagcggcaag gcacttcaga
     1081 tcgtgccggt tgagctcggc gcggcgggcg agatcctcga cgctgccgaa caaccggtcc
     1141 cgacgcgcgg cgacaatgcg ttcggcaccc gttttcgaca gaccttgcac gagcagaaaa
     1201 ccgagtcgaa ctgcgagtgc atcggtgccg gtttccagag tgcaatccca ttcgctggcc
     1261 agcgcgtcca ccggacgcac ttcgacgcca tgccggcgcg cgtcctgcac gagctgtgaa
     1321 ggcgtgtaga aacccatcgg ctggctgttc aagagcgcag cgagaaaggc cgccggctcg
     1381 tggtgcttga gccaggcgga aacgtaggcg agcagcgcga aactcaccga gtgtgactcg
     1441 gggaaaccgt attcgccgaa gccgaggatc tgccggtaga tgcgctcggc gaattccgcc
     1501 gagatgccgt tcttcctcat gccgtccatg agcttcttgt ggaaaggctc cagccctccc
     1561 ttgcggcgcc aggcggccat cgcgcggcgc aattgatccg cttcgcccgg agtgaagcct
     1621 gccgccacca ccgcgagctg catcacctgc tcctggaaaa tcggcacccc gcaggtgcgc
     1681 tcgagaactt ttctcaccgc ttcgttcgga tagtcgatcg ggtccgtgcc ggcacgccgc
     1741 cgcagataag ggtgcaccat gtcgccctgg atcggcccgg gccgcacgat cgcgatctcg
     1801 atcacgaggt cgtagaaatg ctccggtttc agccgcggca gcatcgacat ctgggcgcgc
     1861 gactcgatct ggaacacgcc gatggtgtcg gcgtgccgca tcatctcgta gaccgcgggg
     1921 tcttcctcgc gcagcaggct gtccatggtg atgctgcttc cgcgcagttc gttgacgaag
     1981 tccagcgcgc gccggatggc ggagagcatg ccgagcgcca gcacatcgac cttgagcagc
     2041 cccaggttct ccaggtcatc cttgtcccat tggatgacac tgcgctcggg catcgctgca
     2101 ttttcgatcg gcaccagccg cgcgagcgcc ccccgcgata tgacgaaacc gccgacatgc
     2161 tgtgacaggt ggcgcggaaa cccgagcaaa gcggaggcga gcccgacgag gcgcagcatg
     2221 accgggctct cgggatcgaa gcccgtatcg cgcaggcggg tggcgagctc cctgcggtcg
     2281 tcccaccaag cgatcgattt ggcgatccgg tccacctgca gcgcatcgag cccgagcgct
     2341 ttcccgacgt cgcgcagcgc gctcttgggt tgataagtga tgagcgtcgc cgccagcgca
     2401 gcgcgctcgc ggccgtattt ggcatagaca tattggatga cttcctcgcg ccgctggtgc
     2461 tcgaaatcga cgtcgatatc gggcggctcg ttgcgctcct tcgagatgaa gcgctcgaac
     2521 aatagctcct gcttggccgg atttacctcg gtgattccca gggcgtagca gacggcggaa
     2581 ttggctgccg agccgcgccc ctggcagaga atgtcccggc tgcgggcgta gcggacgatg
     2641 tcgtggacag tcaggaaaaa cggctcgtag cgaagctcgg cgatgagcga cagttcgcgc
     2701 tccatgagat cgcggatctt ctgcgatgct ccgacgggaa agcgccgctg caatccctct
     2761 tcggccagct tgcgcagcca gctcgccggg gtttcgccgt cggggaccag ctcctcggga
     2821 tactcgtaac gcagctcttc cagcgagaaa cgacagcccc cggcaacgcg cacggtttcc
     2881 tccagcaact ccggc
//
LOCUS       DASPZD010000013         6450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1560145, whole genome shotgun sequence.
ACCESSION   DASPZD010000013 DASPZD010000000
VERSION     DASPZD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560145"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..2328)
                     /gene="clpA"
                     /locus_tag="VEM33_00210"
     CDS             complement(52..2328)
                     /gene="clpA"
                     /locus_tag="VEM33_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HYS50285.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPTAAEVL
                     RACAANIEELRKALGDFIAAHTPTVAGTEEIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLHQQGVTRLDVVNYISHGITKTPASKEAKGGGDAEQET
                     AGEEQQSGGALETYTLNLNAQALVGKIDPLIGREREIERVIQTLCRRRKNNPLLVGEA
                     GVGKTAIAEGLARRIVEGQVPEILARCQVYMLDMGALLAGTKYRGDFEQRLKAVLKQL
                     VDNPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGALKCIGATTYNEYRGVFE
                     KDHALSRRFQKIDVPEPSVEETVAILRGLKSRFEAHHGIKYSGNALTSAAELSARYIN
                     DRHLPDKAIDVIDEAGAAQKILPKSKQKKVVGKTEIEEIISKIARIPPASVSQDDRAA
                     LKNLDRDLKAVVFGQDQAIEALVSAIRTARSGLANPQKPIGAFLFSGPTGVGKTEVAR
                     QLAYCMGIELHRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVTKKPYAVLLLD
                     EIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNIIIVMTTNAGAEALNRPVMGFTAK
                     KEPGDESAEIKRLFSPEFRNRLDATISFKSLDHEIILRVVDKFLMQLEVQLHEKKVDA
                     VFTDALREHLAKHGFDPLMGARPMSRLIQDTIRRALADELLFRKLANGGKVTVDIEAE
                     KITLKFVEEKVPEVAPEA"
     gene            complement(2390..2698)
                     /gene="clpS"
                     /locus_tag="VEM33_00215"
     CDS             complement(2390..2698)
                     /gene="clpS"
                     /locus_tag="VEM33_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HYS50286.1"
                     /translation="MATRMREGALLEAKKAKLKPPPLFKVILLNDDYTPMEFVVIVLQ
                     RFFSMTREKATQVMLKVHREGMGICGIYPKDVATTKVEQVVGYARKHQHPLQCVMEET
                     "
     gene            3044..3250
                     /locus_tag="VEM33_00220"
     CDS             3044..3250
                     /locus_tag="VEM33_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYS50287.1"
                     /translation="MPTGTVKWFNDAKGYGFITPDDGGEDLFAHFSAIQMSGFKTLKE
                     GQKVQFEVTQGPKGKQASNIQAAS"
     gene            complement(3402..4616)
                     /locus_tag="VEM33_00225"
     CDS             complement(3402..4616)
                     /locus_tag="VEM33_00225"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HYS50288.1"
                     /translation="MGKIKVKNPIVEMDGDEMTRIIWKTIRERLILPYLDLELKYYDL
                     GVGTRDKTNDQITIDAANATRKYGVAVKCATITPDEGRVKEFNLKQMWRSPNGTIRNI
                     LDGTIFREPIICKNVPRIVPHWNKPVVVARHGFGDQYRATEIKFDGAGTIKLSFQPAG
                     GGAAVEREVFKAPGAGVTLAMFNLDESIKGFARACFNYALQRKYPVYLSSKNTILKVY
                     DGRFMHLFQEIFDADFKKQFDAAGLTYEHRLIDDMVAASLKWSGGYLWACKNYDGDVQ
                     SDTVAQGYGSLGLMTSVLMSPDGKAVEAEAAHGTVTRHYRLHQQGKPTSTNPIASIFA
                     WTRGLIFRGRMDATPDVVRFAETLEKVCVDVVESGKMTKDLAILIRPDHPFLSTDEFM
                     GAVDAELGRRVH"
     gene            4961..5152
                     /locus_tag="VEM33_00230"
     CDS             4961..5152
                     /locus_tag="VEM33_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50289.1"
                     /translation="MRKLISILVATVFAVSSGAALAASHAGAQPMKDDKKSEMKKSDK
                     KADKKAKKTKKEAKKDEKK"
     gene            5222..5662
                     /locus_tag="VEM33_00235"
     CDS             5222..5662
                     /locus_tag="VEM33_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HYS50290.1"
                     /translation="MKRTLRFLGASAWLAASAALAQLPVLELSAGIHLIRAEVAYTVE
                     TRAQGLMFRKHLDSNEGMFFVFPQSEPYCMWMKNTLIPLSVAFLDEKGRIVSISEMQP
                     QTETSHCATAPAKFALEMPAGWFAKKGIKSGATIQGLEKAPAAR"
     gene            complement(5705..6196)
                     /locus_tag="VEM33_00240"
     CDS             complement(5705..6196)
                     /locus_tag="VEM33_00240"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HYS50291.1"
                     /translation="MDWVPAISRWIHLLAGVMWIGLLYYFNFVNVAAAKAAAADGTAA
                     GISKHVMPRALFWFRWAAVVTWLAGAALLGRHFLDAFLFLNKAYYPIGVGAWLGTFML
                     INVWGLIWPNQKKILGIVSASDEEKNKARRVAFLASRTNTMFSIPMLYFMAAGAHSAL
                     YFG"
BASE COUNT         1184 a         1933 c         1987 g         1346 t
ORIGIN      
        1 cgccgcaaaa aacaaaagcc gccctaggcg gcctttgttc gttgcataga ctcaggcttc
       61 cggagccacc tccggcactt tttcttcgac aaacttgagc gtgatttttt ccgcctcgat
      121 atccaccgtg accttcccgc cgttggcgag cttgcggaag agcagctcgt cggcgagcgc
      181 gcggcgaatc gtgtcctgga tgagcctcga catcgggcgc gctcccatca gcggatcgaa
      241 gccgtgcttc gcgaggtgct cgcgcagggc gtcggtgaat accgcatcga cctttttctc
      301 gtgcagctgg acctcgagct gcatgaggaa cttgtccacc acgcgcagga tgatttcgtg
      361 atcgagcgac ttgaacgaga tggtcgcatc cagccgattc cggaactccg gagaaaacag
      421 ccgcttgatt tcggcgctct cgtcgccggg ctccttcttg gcggtgaagc ccatcacggg
      481 gcggttgagc gcctcggccc cggcgttggt cgtcatgacg atgatgatgt tcctgaaatc
      541 tgccttcctg ccgttgttgt cggtgagcgt gccgtgatcc atcacctgaa gcaggatgtt
      601 gaagatgtcc gggtgcgctt tctcgatttc gtcgaggagc agcaccgcgt atggcttctt
      661 ggtgaccgct tccgtcagga gaccgccctg gtcgaaaccc acgtaaccgg gaggtgcgcc
      721 gataagacgc gacaccgcgt gccgctccat gtattcggac atgtcgaagc ggtgcaattc
      781 gatccccatg cagtacgcga gctggcgcgc gacctcggtc tttccgacac ctgttggacc
      841 cgagaaaaga aaggcgccga tcggcttctg cggattggca aggcccgagc gcgcagtgcg
      901 gatcgccgac accagcgctt cgatggcctg gtcctgcccg aacacgaccg ctttcaggtc
      961 gcggtcgagg ttcttgaggg cggcgcggtc gtcctgcgac accgaagcgg gcgggatgcg
     1021 cgcaatcttg gagatgatct cctcgatctc ggtcttgccc acgacctttt tctgcttgga
     1081 cttgggcagg atcttctgcg cggcgccggc ctcgtctatg acgtcgatcg ccttgtcggg
     1141 caggtgccgg tcattgatgt agcgggcaga gagctctgcg gcgctggtga gcgcgttgcc
     1201 gctgtacttg atgccgtggt gcgcctcgaa gcgcgatttc aggccgcgca ggatcgcgac
     1261 cgtctcctcg accgagggtt cgggcacgtc gatcttctgg aagcgccgcg acagcgcgtg
     1321 atccttctcg aatacgccgc ggtactcgtt gtaggtcgtc gcgccgatgc atttcagcgc
     1381 acccgaagag agcgccggtt tcagcaggtt cgacgcgtcg agcgttccgc ccgaggcagc
     1441 acccgcgccg ataagcgtgt ggatctcatc gatgaacagg atcgcattcg ggttgtccac
     1501 cagctgcttc agcaccgcct tcaggcgctg ttcgaaatcg ccgcgatact ttgtacccgc
     1561 gagcagcgca cccatgtcga gcatgtatac ctggcagcgc gcgaggatct cgggaacctg
     1621 gccttcgacg atgcgtcgcg cgaggccttc ggcgatggcc gtcttgccca cgcccgcctc
     1681 gcctacgaga aggggattgt tcttgcgcct ccggcacagc gtctggatga cgcgctcgat
     1741 ctcgcgctcg cgaccgatca gcggatcgat cttgcctacc agcgcctgtg cgttgaggtt
     1801 gagcgtatac gtctcgagcg cgccgccgga ctgctgctcc tcgcccgcgg tttcctgctc
     1861 ggcgtcgccg ccgcccttcg cctccttgga ggccggagtc ttggtgatgc cgtgcgaaat
     1921 gtagttgacg acatcgagac gcgtgacgcc ctgctggtgc aggaaataaa cggcatgcga
     1981 gtctttctcg ccgaagatcg cgaccagcac gttggcgcct gtcacttcct tcttgcccga
     2041 ggactggacg tgcaggatcg cgcgctggat gacgcgctgg aagccgagcg tgggctgggt
     2101 atcgatttcc tcggtgcccg cgaccgtggg ggtgtgcgcg gcgatgaagt ctccgagcgc
     2161 cttcctcaat tcctcgatgt tggcggcgca ggcgcgcagc acttccgcgg ccgtcggatt
     2221 gtccagcaac gcgagcagca ggtgctcgac ggtgatgaac tcgtgccgtt tctggcgcgc
     2281 ctccacgaac gccatgtgga ggctgacttc gagttcctgt gcgatcatct taagaaggcc
     2341 tcttgccggg aaaactcacc ctatcatatg cgccctgacg gggcgcaaat caagtttctt
     2401 ccatgacgca ttgtagcggg tgctggtgct tgcgtgcgta gccgacgact tgctcgacct
     2461 tggtagtggc gacgtctttg gggtagatcc cgcaaatgcc catgccctct cgatggactt
     2521 tgagcatgac ctgggtcgcc ttttcccgcg tcatggaaaa gaacctctgc agcacgatga
     2581 cgacgaattc catcggggtg tagtcgtcgt tcaaaaggat caccttgaat agggggggag
     2641 gtttgagctt ggccttcttg gcctcgagta gagcgccttc tcgcatgcgg gttgccatac
     2701 ctcacttaac ttatgccaga agtttccacc cgtcaaaccg ctagcacccg cagcaggcgg
     2761 aaaagtcacg caccccatgc acaccctttc aatcggaacg cctccagttc agcgtacgca
     2821 catcgctcct aatgtaatcg ctgtcgctgg gctgcgcaac atccaaacgt gtcggcagaa
     2881 caaagggttg acgctctcct gggcacttga cgccgccgcc caaagcgcgt aaaaggcttc
     2941 cctgtgtttg tcctctatgc ggtttgcagg ctgaaattcc tctgctgcgt tgaagtacag
     3001 gcatagttga aagttccccg gttctaaaat tcaggaaggc tctatgccaa caggtacggt
     3061 gaagtggttc aatgatgcta agggttatgg gttcataacc ccggatgacg gcggcgagga
     3121 tttgtttgcg cacttctccg cgattcagat gagcggattc aaaacgctca aggaaggaca
     3181 gaaagtccag ttcgaagtca cgcaaggccc gaagggcaag caggcttcga acatccaggc
     3241 cgcaagctag cggctcgcgc gggcggcgca gccgcccgcg gtcgcaagtc cctcttggtt
     3301 ttcggccccg ggagctccct ggggcccggt cgtctttttt cccatcggcg gtaaagacct
     3361 ctgcgtggag gccgatcctc gacgttctcc cgcgcgctcg gtcagtgcac gcgccgtccg
     3421 agttccgcgt ccacggcccc catgaattcg tcggtggaga ggaaggggtg gtcgggacgt
     3481 atcaggattg ccagatcctt ggtcatcttg cccgattcca cgacgtcgac gcagaccttt
     3541 tccagcgtct ccgcgaatct gaccacgtcg ggcgtggcgt ccatgcgccc acgaaatatg
     3601 agcccgcgcg tccacgcgaa aatcgaggcg attggattgg tcgaagtggg cttgccttgc
     3661 tggtgcagcc ggtagtgccg ggtcacggtg ccgtgtgccg cctcggcctc gaccgccttc
     3721 ccgtcggggg acatcaacac cgaggtcatg agtccgagcg agccgtagcc ttgggcgacc
     3781 gtatcggact gcacgtcgcc gtcgtagttc ttgcacgccc acaggtagcc gccgctccac
     3841 ttgaggctcg cggccaccat gtcgtcgatc agccggtgct cataagtcag gcccgcggcg
     3901 tcgaactgtt tcttgaagtc ggcgtcgaaa atctcctgga acaggtgcat gaagcggccg
     3961 tcgtaaacct tgaggatggt gttcttgctc gagaggtaga cggggtactt gcgctgcaag
     4021 gcgtagttga agcaggcgcg cgcaaaaccc ttgatcgatt cgtcgaggtt gaacatcgcc
     4081 agcgtgacgc cggcgcccgg agccttgaag acttcacgct ccactgcagc gccgccgccg
     4141 gcgggctgga agctcagctt gatcgtgccc gcgccgtcga acttgatctc ggttgcgcga
     4201 tactggtcgc cgaagccgtg gcgggcgacg accacgggct tgttccagtg gggaacgatg
     4261 cgcggcacgt tcttgcagat gattggctcg cggaagatgg tgccgtcgag gatgttgcga
     4321 atggtgccgt tcgggctgcg ccacatctgc ttcaggttga actccttcac gcgcccttcg
     4381 tccggagtga tggtcgcgca tttcacggct acgccgtatt tgcgcgtggc attggccgcg
     4441 tcgatcgtga tctggtcgtt ggtcttgtct ctcgtcccga ccccgagatc gtagtatttc
     4501 agctccaaat cgagataggg aaggatcagg cgctcgcgga tcgtcttcca gatgatccgc
     4561 gtcatttcgt cgccgtccat ctcgacgatc ggattcttga ccttgatttt gcccacggga
     4621 agctcctgta gtctcgcttt ttggaagggg tcggtgctgc gaccttacgg taatcgcggg
     4681 gccgcgaaca cgaggggctt tccgctttga tcgggcggta ttatagccaa ttgcccgagt
     4741 tcctccgctc gccgcgaggc cagggctccc atgatatagg tccgtagctg gttgattttg
     4801 aaaatgaaaa tggattccaa gacctgcggt ggcgaccggg cgtgccgcca agctgacgag
     4861 cttgtcaacc tcggggaggg cagggcgtta gtgggaggga ctggttccgg agcaaaggga
     4921 ctggtcgcaa cctgttcctt aacttcacga agaggaaaac atgaggaagc tgatctccat
     4981 cctggtcgcc acggtgttcg cggtttccag cggcgcggct ctggccgcgt cccacgccgg
     5041 tgcccagccg atgaaggacg acaagaagtc tgaaatgaag aagagcgaca agaaggccga
     5101 caagaaagcc aagaagacca agaaggaagc caagaaggac gaaaagaagt agttgtctct
     5161 cgacgagacc agggcagggt tcgattccct gcctttttta ttttcgtcga ccgcgtccgg
     5221 tgtgaagagg acccttagat tccttggcgc ctccgcgtgg ctcgccgcga gcgccgcgtt
     5281 agcgcagctg ccggtcctgg agctgtcagc ggggattcac cttatccgcg ccgaggtcgc
     5341 ctacacagtc gagacgcggg cgcagggcct catgttccgc aagcatctcg attcgaacga
     5401 gggcatgttt ttcgtgtttc cacaatccga accttactgc atgtggatga agaatacgct
     5461 gatcccgctc tcggtcgctt tcctcgacga gaagggaaga atcgtgagca tctcggaaat
     5521 gcagccgcaa acggaaactt cgcattgtgc gacggctccc gcgaagttcg cgctcgaaat
     5581 gccggcgggc tggttcgcga agaaagggat caagtccggc gcgacaatcc agggactcga
     5641 gaaggctccc gccgcgcgct aaaaaaggct ggcgaatgcc agcctttttt ttgtcgagcc
     5701 cgtcctaccc gaagtaaagc gcgctatgcg cgcccgccgc catgaaatac agcatcggaa
     5761 tggaaaacat cgtattcgtg cgcgaggcga ggaaggcgac gcgccgcgcc ttgttctttt
     5821 cctcgtcgct cgcggagact atgcccagga tcttcttctg gttgggccag atcagccccc
     5881 atacgtttat cagcatgaat gtcccgagcc aagcaccgac cccgatcggg taataggctt
     5941 tgttcaggaa gagaaacgcg tcgaggaaat ggcgccctag caaggccgcg ccggcgagcc
     6001 aggtcacgac cgccgcccag cggaaccaaa acagggctcg gggcatgacg tgcttgctga
     6061 tgccggcggc agtgccgtcg gccgcagccg ctttcgccgc ggcgacattg acgaagttga
     6121 agtagtacag caggccgatc cacatcactc ccgcgaggag atggatccag cgggatattg
     6181 caggtaccca gtccatgtca gcctcccaac gcgtacgaga cgatgttgtg caggatcacg
     6241 gtcaggatca aaccgcatat gaccgtcccc caaagtgaat cgagcggatt cttcatggtc
     6301 gttctcccga tgatcagggc cgcagccccg cgccccgcgg tattctctgt ccaagcggcc
     6361 ccgattgcaa gcggccgctt ctgcgttctc gattcctatc gtgaacgctc gacgcgtgct
     6421 tcggcgccgc cctctgcaaa cgtgatatcg
//
LOCUS       DASPZD010000014         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1322829, whole genome shotgun sequence.
ACCESSION   DASPZD010000014 DASPZD010000000
VERSION     DASPZD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322829"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..371)
                     /locus_tag="VEM33_00245"
     CDS             complement(66..371)
                     /locus_tag="VEM33_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50292.1"
                     /translation="MKIRSVVPLFALALALPAVAQQYPAKPVRLLIGYAAGSSTDIVG
                     RVMADRLGAYWKQNVYVETRAGAAGNLAADPPQADHAERLSGELGADELVALPLALV"
     gene            complement(383..1306)
                     /locus_tag="VEM33_00250"
     CDS             complement(383..1306)
                     /locus_tag="VEM33_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYS50293.1"
                     /translation="MDNPFQKIKRIQARPFTSLPAKFRKKRRTTWSDPNRGGAEVDSF
                     LEGPSFDRDGNLWCVDIPFGRVFRIDPKGEWELVVQYDGWPNGLKIHKGGWIFIADYK
                     RGLMLLDAKSAKIETYLPTSFSEGFKGLNDLHFADNGDLYFTDQGQTGIADPSGRVYR
                     LRANGELQRLVDNAPSPNGITLNKANTHVYVAITRSQQIWRLPLMAGGTLSKTGVAIQ
                     LSGGHAGPDGIEADEEDGLAVCHLGVGIWRFDSNCLPTHLVDPGKQPRLMTNIAYGGA
                     KRSVLHITDSLNGEIVTAEMPVPGKKMFAHR"
     gene            complement(1383..>2286)
                     /locus_tag="VEM33_00255"
     CDS             complement(1383..>2286)
                     /locus_tag="VEM33_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50294.1"
                     /translation="KVDILAGFVLTPNALAAADVSAEAKKFMVVMNAATAIVTTKSPY
                     MARTSLTVPQLNETFGAWAYRNGTRKLYTMVSDYGPGHDAEGAFQRGFKEAGGEVVGS
                     VRIPVANPDFFPFVQRAKDLNPEAMYVFIPGGAQPAAFGKALVDLGIDSKKTRILGQG
                     EITEENALKVMGDAALGLVTAFHYDHNHKSATNTQFVSAYNAAWGRNPDFFSVGGWDG
                     MHLIYEALKKTGGKTDAEALIAAAKGMKWESPRGPIAIDPETRDIVQTVYIRRVEKVG
                     GRLVNVEIDKIENVKDPVKERMKK"
BASE COUNT          360 a          725 c          761 g          440 t
ORIGIN      
        1 ccgccgagca caccatcacc ctgatgatgg cgctgtcccg gcacgtcgcc ccggctgacg
       61 ccagcctaca ccagggcaag tgggagcgca acaagttcgt cggcacccag ctcgccggaa
      121 agacgctcgg cgtgatcggc ctggggcggg tcggccgcga ggttgcccgc cgcgccggcg
      181 cgcgtctcga catacacgtt ctgcttccag taggcgccga gccggtcggc catcacgcgc
      241 ccgacgatgt cggtcgagct tcccgccgca tagccgatca gcaggcgcac gggcttggcc
      301 gggtattgct gcgcaacggc cggcagggca agagcgagag cgaaaagcgg gacgacgctg
      361 cgaatcttca tgggccactc cttcatcgat gcgcgaacat cttctttccc ggcaccggca
      421 tctcggcggt gacgatctcg ccgttcagcg agtcggtgat gtggagcacg ctgcgcttcg
      481 cgccgccgta ggcgatgttg gtcataaggc gaggttgctt gcccgggtct acgagatggg
      541 taggcaggca gttcgagtcg aagcgccaga tgcccacgcc gaggtggcac acggcgagcc
      601 cgtcctcctc gtccgcctcg atgccgtcgg ggccggcgtg gccgcccgag agctggatcg
      661 cgacgccggt cttggaaagc gtgcctccgg ccatgagagg aagccgccag atctgctgcg
      721 agcgcgtgat ggcgacgtac acgtgcgtgt tggccttgtt gagcgtgatg ccgttgggtg
      781 agggtgcgtt gtccaccagg cgctgcagct cgccgttcgc gcgcaggcgg tatacgcgcc
      841 cgctaggatc ggctatgccg gtctgtccct ggtcggtgaa atacagatcg ccgttgtcgg
      901 cgaagtgcag gtcgttcagg cccttgaagc cctcgctaaa cgaggtgggg aggtaggttt
      961 cgatcttcgc ggatttcgcg tcgagcagca tcaggccgcg cttgtaatcc gcgatgaaaa
     1021 tccagccgcc cttgtggatc ttcaggccat tcggccagcc gtcgtactgg acgacgagct
     1081 cccattcgcc tttcgggtcg atcctgaata cgcgcccgaa cggaatgtcc acgcaccaca
     1141 ggtttccgtc gcggtcgaag gaaggcccct cgaggaagct gtccacctcc gccccgcccc
     1201 ggttggggtc ggaccatgtg gtgcggcgct tcttgcggaa cttcgcgggc agcgaggtga
     1261 acggccgcgc ctggatgcgc ttgattttct ggaacgggtt gtccatcgcg gctccgcagg
     1321 aagagcggag gataactcaa gtcccggacg atcggcccga ttttccggcg ggctcgatcg
     1381 cgctacttct tcatccgctc cttgacagga tccttgacgt tctcgatctt gtcgatctcg
     1441 acgttgacga ggcgtccgcc gaccttctcg acgcggcgga tatataccgt ctggacgatg
     1501 tcgcgagttt ccggatcgat ggcgatcggg ccgcgcgggc tttcccattt catgcccttg
     1561 gcggccgcga tgagggcttc ggcgtcggtt ttgccgcctg tcttcttcag ggcctcatag
     1621 atgaggtgca tcccgtccca gccgccgacg ctgaagaagt ccgggttgcg tccccaggcc
     1681 gcgttgtagg cagagacgaa ctgcgtattc gtcgcggact tgtggttgtg atcgtagtga
     1741 aacgccgtga ccaggccgag cgcggcatcg cccatgacct tcagcgcgtt ctcctcggtg
     1801 atttcaccct ggcccaggat tcgcgtcttc ttcgaatcga tgccgagatc cacgagcgcc
     1861 ttgccgaagg cggccggctg cgcgcccccg gggatgaaaa cgtacatcgc ctccgggttg
     1921 aggtctttcg cgcgctgcac gaacgggaag aagtcgggat ttgccacggg aatgcgcacc
     1981 gagccgacga cctcgccgcc ggcctccttg aacccgcgct ggaacgcgcc ctccgcgtcg
     2041 tgtccgggac cgtagtccga caccatcgta tacagcttgc gggtgccgtt cctgtaggcc
     2101 caggcgccga aggtctcgtt cagctgcggc acggtgagcg aagtgcgcgc catgtagggc
     2161 gacttggtcg tgacgatcgc ggtggccgcg ttcatcacca ccatgaattt cttcgcttcg
     2221 gcggatacgt ccgcggcggc gagggcgttg ggcgtgagca cgaacccggc gaggatatcg
     2281 accttg
//
LOCUS       DASPZD010000015         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1677881, whole genome shotgun sequence.
ACCESSION   DASPZD010000015 DASPZD010000000
VERSION     DASPZD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677881"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VEM33_00260"
     CDS             complement(<1..232)
                     /locus_tag="VEM33_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYS50295.1"
                     /translation="MTRQTENRGKANRKLMGRLLVVVAVMFGFGFALVPFYEKICEVT
                     GIRNVFQPDAVAAQNTQVDTTRNISVEFDANTQ"
     gene            complement(229..342)
                     /locus_tag="VEM33_00265"
     CDS             complement(229..342)
                     /locus_tag="VEM33_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HYS50296.1"
                     /translation="MMPAREQRRSNRRTALVLGSIALVFFVAIMLKYVMLK"
     gene            complement(339..1928)
                     /gene="ctaD"
                     /locus_tag="VEM33_00270"
     CDS             complement(339..1928)
                     /gene="ctaD"
                     /locus_tag="VEM33_00270"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HYS50297.1"
                     /translation="MSAVTDSGHAHDEHGPYGGIMRWVTTTNHKDIGTLYLWFSFTMF
                     LVGGVLALAIRAELFQPGLQFWHPEFFNQLTTMHGLIMVFGAIMPAFVGFANWQVPLM
                     IGAPDMAFARLNNWSFWMLPVAATLLVGSFFVPGGATAAGWTIYAPLSTQMGPGMDLA
                     IFALHIMGASSIMGSINIITTILNMRAPGMTLFRMPLFVWTWLITAYLLIAVMPVLAG
                     AITMLLTDRHFGTSFFNAAGGGDPVMFQHIFWFFGHPEVYIMILPGFGIVSQVIPAFS
                     RKPLFGYSSMVYATASIAVLSFIVWAHHMFTVGMPTAGILFFMYATVLIAVPTGVKVF
                     NWLTTMWRGSMSFETPMLFAVGFIFVFTMGGFTGLILAIMPIDIQLQDTYYVVAHFHY
                     VLVAGSLFSLFAGFYFWVPKWTGRMYDEKLGKWHFWLSMIFFNVTFFPMHFLGLAGMP
                     RRIPDYALQFAGFNEIASIGALGFGLSQILFLWIALKVTVLGQGEKAGQRPWDGADTL
                     EWTHLPSPAPYHSFHEQPVLG"
     gene            complement(1945..3057)
                     /gene="coxB"
                     /locus_tag="VEM33_00275"
     CDS             complement(1945..3057)
                     /gene="coxB"
                     /locus_tag="VEM33_00275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYS50298.1"
                     /translation="MVNGTGAALLALAGAAEAAYQWNLPTPVTRVAGEIYSLHLVMMG
                     IILVIFIGVFGVMFYSIYAHRKSVGHKAENFHENIVVEIIWAIIPFVILIGVAWPATK
                     SILNLKDTASPDITIKATGYQWKWGYDYLKGEGEGISFLSNLSTPQDQITGSAPKGEH
                     YLLEVDNHMVVPVGKKVRVLLTSNDVIHAWMVPAFGVKQDAIPGFVRDTWFRAEKEGI
                     YRGQCAELCGKDHGFMPIVVEVLSAAKYTAWVGEQKMKAAAAGDDPNKKWNLADLKAR
                     GEKVYAANCIACHQANGQGVKGTFPALDGSQLVNGPRGRQMEIVLNGKRGTAMAPFGK
                     QLSDTDVAAVITFTRNNWGNKTGEVIQPGEIRSARK"
     gene            complement(3069..4001)
                     /gene="bioC"
                     /locus_tag="VEM33_00280"
     CDS             complement(3069..4001)
                     /gene="bioC"
                     /locus_tag="VEM33_00280"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102130 - malonyl-CoA methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HYS50299.1"
                     /translation="MNPQAFGLDSVQVRRAFERAAAAGGDAAVLQREVERRMFERLDY
                     IRCRPHRVLDSGCGVGRGLKLLRRRYPEADLFGMDFAPGVLSGAKREESLFERARGLV
                     SGSRRFHLCADFARLPLRAASVDMVWSNLALAWAGDPLAALREVHRVLIPGGLLMFSS
                     YGPDTLKELKAAFGAGSPGSGCHVHSFIDMHDLGDMLVASGFAAPVMDMEVITLTYSE
                     VPALLRDLKASGETYAARDRQRGLMGRRAWERMLASYERERKEGRLPATIEVIYGHAW
                     KGEPRTTVDGRRIIEVELEAKKTGPLVRPEPTRT"
BASE COUNT          798 a         1286 c         1289 g          740 t
ORIGIN      
        1 gctgggtgtt ggcgtcgaat tcgaccgaga tattgcgcgt ggtgtccact tgagtgttct
       61 gcgccgccac ggcgtccggc tggaacacgt tccggatgcc cgtcacctcg cagatcttct
      121 cgtagaaggg aacgagcgcg aagccgaaac cgaacatcac ggccacgacc accagcagcc
      181 ttcccatcag tttccggttg gccttcccgc gattctcggt ctgccgtgtc atttgagcat
      241 gacgtacttg agcatgatcg ccacaaagaa gacgagcgcg atcgagccga gcaccagcgc
      301 ggtcctgcgg ttgcttcgcc gctgctcgcg tgcaggcatc atcccaacac cggctgctcg
      361 tgaaagctgt gatagggagc gggactcggc agatgcgtcc actcgagcgt gtcggcgccg
      421 tcccagggcc gttgccccgc cttctcgcct tgcccgagca cggtcacttt cagggcgatc
      481 cacaggaaca ggatctggga cagcccgaag cccagcgcgc cgatcgaggc gatctcgttg
      541 aacccggcga actgcagcgc gtagtccgga atcctgcgcg gcatgccggc caggcccagg
      601 aagtgcatcg ggaagaaagt gacgttgaag aagatcatcg acagccagaa gtgccatttc
      661 ccgagcttct cgtcgtacat cctgccggtc catttgggca cccagaagta gaatcccgcg
      721 aagagcgaaa acagcgaccc ggcgaccagc acgtagtgga aatgcgccac gacgtaatac
      781 gtgtcctgga gctggatgtc gatcggcatg atcgcgagga taaggccggt aaatccaccc
      841 atcgtgaata cgaaaataaa gcccactgcg aacagcatcg gcgtctcgaa gctcatcgag
      901 ccgcgccaca tggtcgtgag ccagttgaac accttgacgc cggtgggtac cgcgatcagc
      961 accgttgcgt acatgaagaa caggatcccc gccgtgggca tgccgacggt gaacatgtgg
     1021 tgcgcccaga cgatgaacga gaggaccgcg atcgaggcgg tcgcatacac catcgaggaa
     1081 tagccgaaca agggtttcct cgagaacgcc ggaatcacct ggctgacgat gccgaagccc
     1141 gggaggatca tgatgtagac ctcggggtgg ccgaagaacc agaagatgtg ctggaacatc
     1201 acggggtcgc cgccgcccgc cgcgttgaag aaggacgtgc cgaaatggcg gtcggtcaac
     1261 aacatcgtga tggcgcctgc gagcaccggc atcaccgcga taaggaggta cgcggtgatg
     1321 agccaagtcc aaacgaaaag cggcatccgg aagagcgtca tccccggtgc gcgcatgttg
     1381 aggatcgtgg tgatgatatt gatcgagccc atgatcgagg aggcgcccat gatgtggagc
     1441 gcgaagatcg cgaggtccat ccccggtccc atctgagtcg agagcggcgc gtagatggtc
     1501 cagccggcgg cagtcgcgcc cccgggcacg aagaacgatc cgacgagcag ggtcgccgcc
     1561 acgggaagca tccagaagct ccagttgttc agccgcgcaa aagccatgtc gggagcgccg
     1621 atcatcagcg gcacctgcca gttggcgaac ccgacgaagg ccggcatgat cgcgccgaac
     1681 accatgatga gcccgtgcat cgtggtgagc tggttgaaga actccggatg ccagaactgc
     1741 aggcccggct ggaacagctc ggcgcggatg gcgagggcga gcacgccgcc gaccaggaac
     1801 atggtgaaac tgaaccacag gtaaagcgtg ccgatgtcct tgtggttggt cgtagtgacc
     1861 cagcgcatga tgcccccgta gggaccatgc tcgtcgtggg cgtggcctga atcggtgact
     1921 gcgctcattg gctgctccta gaggtcactt ccgcgccgac ctgatctcgc ccggctgaat
     1981 gacttcgccg gtcttgttcc cccagttgtt gcgggtgaag gtgatgaccg cggcaacatc
     2041 ggtgtcggag agctgcttgc cgaacggcgc catcgccgtg ccgcgcttcc cgttcagcac
     2101 gatctccatt tgccgccccc tcggtccgtt gacgagctgg gaaccgtcga gcgcggggaa
     2161 ggttcccttg accccctgcc cgttcgcctg atggcaggcg atgcaattcg cggcgtagac
     2221 cttctcgccg cgggccttca ggtcggcgag attccatttc ttgttcggat cgtcgcccgc
     2281 ggcagcggcc ttcattttct gctctcctac ccaggcggtg tactttgcgg ccgagaggac
     2341 ttcgacgacg atcggcatga acccgtggtc cttgccgcaa agctccgcgc actggccgcg
     2401 gtagatgcct tccttttccg ccctgaacca ggtatcgcgc acgaagccgg gaatcgcgtc
     2461 ctgtttcacg ccgaacgccg gaaccatcca ggcgtgtatg acgtcgttgg aggtgaggag
     2521 gacgcgcacc ttcttgccca cgggcacgac catgtggttg tccacctcca ggagatagtg
     2581 ctcgcctttg ggtgcgctgc ccgtgatctg gtcttgcgga gtggacagat tcgagaggaa
     2641 tgatatgccc tccccctcgc ccttcaggta gtcgtagccc catttccact gatagccggt
     2701 tgccttgatg gtgatgtcgg ggctagcggt gtccttgagg ttcaggatcg acttggtcgc
     2761 cggccaggcc acgccgatca gtatcacgaa cgggatgatg gcccagatga tctccacgac
     2821 gatgttctcg tggaaattct cggccttgtg gccgacagac ttgcggtgag cgtagatcga
     2881 atagaacatc acgccgaaca cgccgatgaa gatcacgagg atgatcccca tcatgacgag
     2941 gtgcaggctg tagatctcgc cggcgacgcg cgtcaccggg gtcggcaggt tccactgata
     3001 cgccgcttcg gcggccccgg ccagcgcgag gagcgcagcg ccggttccgt tgaccatcct
     3061 gttggtcatc atgtcctcgt tggttctggt cgaacaagcg ggcccgtttt tttcgcttcc
     3121 aattctactt ctatgatccg ccttccgtcg acggtcgtac gcggctcgcc tttccatgcg
     3181 tgcccgtaga tcacctcgat tgtcgccggc agcctgcctt ccttgcgctc gcgctcatag
     3241 gaggcaagca tgcgctccca cgcgcggcgt cccatgaggc cgcgctgccg atcgcgcgcc
     3301 gcgtaggttt cgccggaggc tttcaggtca cgcaggagcg ccgggacctc ggagtaggtc
     3361 agcgtgatga cttccatatc catgacgggc gcggcaaatc cgctcgcgac cagcatgtcg
     3421 ccgaggtcgt gcatgtcgat gaaggaatgc acatggcacc cggacccggg cgagcccgcg
     3481 ccgaaggccg ctttgagctc cttcagcgtg tcgggcccgt agctcgagaa catcagcagc
     3541 ccgccgggga tgagcacgcg gtgaacctcg cgcagcgccg cgagcggatc gcccgcccag
     3601 gcaagcgcaa gattcgacca caccatgtcc acgctcgcgg cgcgaagcgg caggcgcgca
     3661 aagtccgcgc acagatgaaa gcgcctggag ccggacacga ggccccgggc gcgctcgaag
     3721 agggactctt cccgtttcgc cccgctcagg acgccgggcg cgaagtccat gccgaaaagg
     3781 tccgcctcgg gatagcggcg ccgcagcagc ttcaggccgc gtcccactcc gcagccggag
     3841 tcgagcacgc gatgcggccg gcagcgtatg taatcgagcc gttcgaacat gcgccgctcg
     3901 acttcccgct ggagcaccgc ggcgtcgccg cccgccgcag cggcacgctc gaaggctcgc
     3961 cgaacctgta cagagtccag cccgaatgcc tgtgggttca tctggagtgg acaataagtt
     4021 cgcaatacat tatataagcc gtagtttaca atcatcgcct gcgaaattcc gtccaatcaa
     4081 ccgggttcat agctatggtc gtagcaatcg tat
//
LOCUS       DASPZD010000016         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_590132, whole genome shotgun sequence.
ACCESSION   DASPZD010000016 DASPZD010000000
VERSION     DASPZD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590132"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1380)
                     /gene="folC"
                     /locus_tag="VEM33_00285"
     CDS             complement(82..1380)
                     /gene="folC"
                     /locus_tag="VEM33_00285"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HYS50300.1"
                     /translation="MPGRSLPEWLDYIERIHPKSIQLGLERVAKVRDVLGRSSSAALF
                     TVAGTNGKGSTCAMLEAILLAAGYRVGLYTSPHLLRYNERVRLDGVPSSDARLSAAFE
                     TVERARGDIKLTYFEFGTLAAWEIFAAEALDAVILEVGLGGRLDAVNAFETDCALLTS
                     VDLDHTDYLGDTRERIGSEKAGIFRGRKPAVVADPDPPRSVLDHAGAVGADLWLIGRD
                     FGYRKQGQQWMSWGRGVRRGGLALPALRGDRQLANASAAIAALDALRARLPVGMQDIR
                     NGFAHVDLPARFQVLPGRPTVVLDVAHNPQAAGVLAENLAGMGFYPDTYAVFGMLRDK
                     DIDGVCRALKDRISIWFAADLAVPRGAGAQTLVDALSRAGVGGNVICCKNPLEAFAAA
                     RKRASENDRIVVFGSFHTVADVMQGIEAARIGVPGARQRS"
     gene            complement(1398..2267)
                     /gene="accD"
                     /locus_tag="VEM33_00290"
     CDS             complement(1398..2267)
                     /gene="accD"
                     /locus_tag="VEM33_00290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HYS50301.1"
                     /translation="MSWLQKLLPPKINRTGTGAKKNVPEGLWSKCPSCAAVLYATDLE
                     KNANVCPKCGYHNRISARGRLDLLLDPEGRFEIGVEVASVDGLKFKDDKRYPDRLEEA
                     EKATGETEALVVMQGAIKNVPLIAAAFEFEFMGGSMGSAVGERFVRGVHACLDQQLPL
                     VCVTATGGARMQEGLFSLMQMAKTTAALTQLTARKLPFITVLTDPTMGGVSASFAMIG
                     DIVIAEPRALIGFAGPRVIEQTVREKLPEGFQRAEFLLEKGAIDMIVDRRQMRDKLAN
                     LLTLLQRQALPAN"
     gene            complement(2264..>2521)
                     /locus_tag="VEM33_00295"
     CDS             complement(2264..>2521)
                     /locus_tag="VEM33_00295"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYS50302.1"
                     /translation="GVTGAKHLDLDDVAAKLPAIRGKTRLPVGVGFGIRDGQTARAVA
                     QIADAVVIGSRIIQEMEQATPKTCVEKAATLLKEIRRAIDA"
BASE COUNT          457 a          839 c          778 g          447 t
ORIGIN      
        1 cagccgccgg cgcgcgcggc gcttaagcag gttggcgtcg tctgccaatg gaaaggaccc
       61 caaatgaacc gcgaattttc ctcaagacct ctgccgcgct cccggaactc cgatccgggc
      121 agcctcgatc ccttgcatga cgtcggctac ggtgtgaaac gatccgaaga ccacgattct
      181 atcattttcg ctcgcccgct tccgcgcggc ggcaaaggct tcgaggggat tcttgcaaca
      241 aatcacgttg cctccgacgc ccgcgcgcga gagggcatca accaaggtct gcgctccggc
      301 gccgcgcggc accgcaaggt cggccgcaaa ccaaatcgaa attctgtctt tcaacgcccg
      361 gcagacgccg tcgatgtcct tgtcccgcaa catgccgaaa acggcatagg tatccggata
      421 gaagcccatc ccggcgaggt tttccgcgag cacgcccgct gcctgcggat tgtgcgcgac
      481 gtccaaaacg acagtcggtc gaccgggaag tacctggaac cgcgcgggaa gatccacgtg
      541 cgcgaaaccg tttcggatgt cctgcatacc gactggaaga cgcgcgcgca acgcatccag
      601 cgcggcaatg gccgcgctcg cgttcgcaag ctggcggtcg cccctgagcg cggggagcgc
      661 aagcccgccc cggcgcacgc cccgtcccca agacatccat tgctgtcctt gcttccggta
      721 gccgaaatcc cggccgatca accaaaggtc cgcacccacc gcacccgcgt ggtcgagaac
      781 gctgcggggc ggatccggat ccgcgacgac agcgggttta cgtccacgga aaatcccggc
      841 tttttcggac ccgatgcgct cgcgcgtgtc gccaaggtag tccgtatgat ccagatcgac
      901 ggaggtcaac aacgcgcagt ctgtttcgaa ggcgttgacc gcatccaggc gccctcccag
      961 gccgacctcc agtatcacgg cgtcgagtgc ctcggccgcg aaaatctccc acgcagccag
     1021 ggtaccgaac tcgaaatacg tgagcttgat atcaccgcga gcacgctcga ccgtttcgaa
     1081 cgctgcagac aggcgcgcgt cgctcgaggg cacgccgtcg aggcgaacgc gctcgttgta
     1141 acgaaggagg tgcggcgaag tgtacagacc caccctgtat ccggcggcga gcaggatcgc
     1201 ttccagcatc gcgcaggtcg agcccttgcc gttggtgccg gcgacagtga aaagcgccgc
     1261 cgagctcgac cttccgagga cgtcgcgcac tttcgccacg cgctccagcc cgagctggat
     1321 ggatttcgga tggatgcgct cgatgtagtc gagccactcg gggaggctcc ggccgggcat
     1381 gaaacggcga ccagcgatca gtttgccggg agggcctgcc gctgcaggag cgtaagaaga
     1441 ttcgcgagct tgtcgcgcat ctggcgccgg tccacgatca tgtcgattgc acccttctcc
     1501 agcaagaact cggcgcgctg gaagccttcg gggagttttt cgcgtacggt ctgctcgatg
     1561 acgcgcggcc ccgcgaaacc gatgagcgcg cgtggctcgg caatcacgat gtcgccgatc
     1621 atggcgaagc ttgcagacac tccgcccatg gtcgggtcgg tgaggaccgt gatgaacggg
     1681 agcttgcgag cagtgagttg cgtgagggcc gctgtggtct tggccatctg catgagcgag
     1741 aacagccctt cctgcatgcg cgcgccgccg gtcgccgtga cgcacacgag cggcagttgc
     1801 tggtcgaggc aggcgtgcac gccgcgcacg aagcgttccc ccacggccga acccatcgac
     1861 ccgcccatga actcgaactc gaaagcggcg gcaatgagag gcacgttttt tatcgccccc
     1921 tgcatcacca ccaaagcctc ggtttcgccg gtggcttttt ccgcttcctc gagccgatcg
     1981 gggtaacgct tatcgtcctt gaatttcagg ccgtcaaccg acgcgacctc cacgccgatc
     2041 tcgaagcgcc cttccgggtc gagaaggaga tcgagccgcc cccgagccga aatgcggttg
     2101 tgatagccgc acttggggca gacattggcg ttcttttcga ggtcggtcgc atacaggact
     2161 gccgcgcaag aggggcactt gctccacagg ccttccggga cgtttttctt cgcgccggtt
     2221 ccggtgcggt tgatcttggg tggaagcagc ttctggagcc agctcatgcg tcgatcgccc
     2281 tgcgaatttc cttgagcaag gtcgcggcct tctccacgca ggtcttggga gtggcttgtt
     2341 ccatctcctg gattatccgg ctgccgatca cgacggcgtc ggcgatctga gccacggcgc
     2401 gcgcggtctg tccatcgcgg atgccgaatc ccacccctac cggaagcctc gtcttgccgc
     2461 ggatcgccgg caacttcgcg gcaacatcgt ccaggtccag gtgcttcgcc ccggtcacgc
     2521 c
//
LOCUS       DASPZD010000017         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1438656, whole genome shotgun sequence.
ACCESSION   DASPZD010000017 DASPZD010000000
VERSION     DASPZD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438656"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..>2054
                     /gene="acs"
                     /locus_tag="VEM33_00300"
     CDS             254..>2054
                     /gene="acs"
                     /locus_tag="VEM33_00300"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HYS50303.1"
                     /translation="MADATIESVLQEDRLFPPPPEFVKQANVSGAAAYQALCDEANRD
                     FEGFWGRLGKEHVLWHKPFTKVLDESNAPFFKWFHDGELNASYNCLDRHLKTQGDKTA
                     IIFEADDGKVTKITYSELYHKVCQFANALKALGIKNGDRVIIYMPMSIEVVTAMQACA
                     RIGATHSVVFGGFSAKSLQERIIDAGAVAVITADGQMRGGKEIPLKPAVDEAFALGGC
                     EGVKNVVVYKRTGTPVAFQAPRDKWWHDAIKGQADTCEPTWVNAEHPLYILYTSGSTG
                     KPKGVQHSTGGYLLMTILTMKWVFDYRPTDVFWCTADVGWVTGHSYCTYGPLAVGATE
                     VMFEGVPVWPDAGRFWKMIQDHKVTVFYTAPTAIRTLIKAGGELPKKYNLKSLRILGT
                     VGEPINPEAWMWYYNTVGDKCCPIVDTWWQTETGAHMITPLPGVHALKPGSCTMPLPG
                     IMAAIVDETGHDVEKGKGGILVIKRPWPSMIRTIWGDPDRYKKSYYPEDFKGKYYLAG
                     DGASRDKDGYFRIMGRIDDVLNVSGHRLGTMEIESALVANVKLVAEAAVVGRPDEMTG
                     EAVCAFVVLKGPRPSGDEAKRIAQELRDWVGKEI"
BASE COUNT          413 a          627 c          675 g          339 t
ORIGIN      
        1 caacaaatcc agatggcacg ggcgttgcga atacgctgct gtcgcttgga gaaatcgacg
       61 ctggaatgaa gagcgtcgca ggatcaccgt ccgcctcaac tcctccctcc ctcccaggcg
      121 gacgggctcg ggcccgcagg agatcaacct cctgcgggtc ttttttttcg gcgtttgcga
      181 gatgcgttgc ctggttgcga agcccgcgcc ggggccggta atatcagcac ttacacaggg
      241 gggagatctg caaatggccg acgcaacgat cgagtccgtg ctccaggagg accggctgtt
      301 cccgccgcct ccggagttcg tcaagcaggc caacgtatcc ggcgccgcgg cctaccaggc
      361 gctgtgcgac gaggccaacc gcgatttcga aggcttctgg gggcggctcg gcaaggagca
      421 cgtgctctgg cacaaaccgt tcaccaaggt cctcgatgaa tcgaacgcgc cttttttcaa
      481 gtggttccac gacggcgagc tgaacgcgtc ctacaactgc ctcgaccggc acctgaagac
      541 gcagggcgac aagaccgcga tcatcttcga ggcggacgac ggcaaggtga cgaagatcac
      601 ctacagcgag ctctatcaca aggtctgcca gttcgcgaat gcgctcaagg ccctcggcat
      661 caagaatggt gatcgcgtca tcatctacat gccgatgtcc atcgaagtgg tgacggcgat
      721 gcaggcctgc gcgaggatcg gcgcgacgca ctcggtcgtt ttcggcggct tctcggcgaa
      781 gagcttgcag gagcgcatca tcgacgccgg cgctgtcgcg gtcatcacgg cggacgggca
      841 gatgcgcggc ggcaaggaaa tcccgctcaa gcccgcggtg gacgaagcct tcgctctggg
      901 cggctgcgaa ggcgtgaaga acgtggtcgt gtacaaacgc accggaactc cggtcgcctt
      961 tcaggcgccg cgcgataagt ggtggcacga tgcgatcaag ggccaggccg atacctgcga
     1021 gccgacctgg gtgaacgcgg agcatccgct ctacatcctg tacacatcgg gttcgaccgg
     1081 aaagccgaag ggcgtgcagc actccaccgg cggctacctg ctgatgacga tcctcaccat
     1141 gaagtgggtg ttcgactaca ggcccaccga cgtattctgg tgcaccgcgg acgtgggctg
     1201 ggtcaccggg catagctact gcacctacgg cccgctcgcg gtcggcgcga ccgaagtgat
     1261 gttcgaaggc gtgccggttt ggccggacgc cgggcgcttc tggaaaatga tccaggacca
     1321 caaggttacg gtgttctaca ccgcgcccac tgcgatccgc acgctcatca aggcgggagg
     1381 cgagcttccg aagaaataca atctgaaatc gctgcgcatc ctcggcacgg tcggcgagcc
     1441 gatcaatccc gaggcgtgga tgtggtacta caacaccgtg ggcgacaagt gctgccctat
     1501 cgtcgatacg tggtggcaaa ccgagaccgg cgcgcatatg atcacgccgc ttcccggcgt
     1561 gcatgccttg aagcccggct cgtgcacgat gccgctcccc ggcatcatgg cggcgatcgt
     1621 ggacgagacc ggccacgacg tcgaaaaggg gaagggcggg atcctcgtca tcaagcgccc
     1681 gtggccttcg atgatccgca cgatctgggg cgacccggat cgctacaaga aaagctatta
     1741 ccccgaggac ttcaagggca aatactacct cgcgggcgac ggcgcgagcc gcgacaagga
     1801 cggatacttc cgcatcatgg gccgcatcga cgacgtgctg aacgtctcgg ggcaccgact
     1861 cggcacgatg gagatcgaat cggcgctggt ggcgaacgtg aagctcgtcg cggaagcggc
     1921 ggtggtcggc aggccggatg aaatgacggg cgaggccgtg tgcgcgttcg tcgtgctgaa
     1981 aggcccgcgg ccttcgggcg acgaggcgaa aaggatcgcc caggaactgc gcgactgggt
     2041 cggcaaggag atcg
//
LOCUS       DASPZD010000018         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1795446, whole genome shotgun sequence.
ACCESSION   DASPZD010000018 DASPZD010000000
VERSION     DASPZD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795446"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1187)
                     /gene="rlmN"
                     /locus_tag="VEM33_00305"
     CDS             complement(105..1187)
                     /gene="rlmN"
                     /locus_tag="VEM33_00305"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYS50304.1"
                     /translation="MSVNLLDLDSEGLAAFVAGLEEKPFRARQVKRWMHRLGESDFAR
                     MSDIAKPLREKLLRAACVSPPRIVSDSSARDGTRKWLLDVGSGNAVEAVFIPETNRGT
                     LCISTQAGCTLACQFCSTGRQGFNRNLSVSEIIGQLWLANRELGATPGGGRVISNVVL
                     MGMGEPLLNFDNTVAALKLMIDEDAYGLSRRRVTLSTSGIVPMIDRLAAECPVALAVS
                     LHAPNDALRDTLMPINRKYPLAELLAACRRYLERAPRDFITFEYVMLEGVNDSEEHAR
                     QLVELVSRVPCKINLIPFNPFPSSGFVRSPRRVIERFGELLSASGIITTTRRTRGDEI
                     DAACGQLAGKVHDRTRRPVRLEGTGS"
     gene            complement(1188..1613)
                     /gene="ndk"
                     /locus_tag="VEM33_00310"
     CDS             complement(1188..1613)
                     /gene="ndk"
                     /locus_tag="VEM33_00310"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYS50305.1"
                     /translation="MAVERTLSIVKPDAVAKNAIGQIYSKFEQAGLKIVAARMRYLSQ
                     REAEGFYAVHRERPFFRDLVRFMISGPIMVQVLEGEDAIAKNRDLMGATDPKKAKKGT
                     IRADFAESIDANAVHGSDGPETARNEIAYFFSASEIFSR"
     gene            1981..2655
                     /locus_tag="VEM33_00315"
     CDS             1981..2655
                     /locus_tag="VEM33_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYS50306.1"
                     /translation="MRVLIVEDDNALADGLMRTLRQSGYAVDHAGSGELALRACAEEH
                     YDLVVLDVSLPGIDGFEVLRQLRRDNHSGSILILTAHDAEADRVRGLDLGADDYVAKP
                     FSLPELEARVRALIRRSQAVKSPKLRFGRLIVDTVARRASIGRDLLDLTPREWAVLEY
                     LLMRVGQVVSKEHMLQAMCSWDDSLTHNAIEVYISRLRSKLHDAGIRIRTVRGFGYMV
                     EDPDAV"
     gene            2645..>2907
                     /locus_tag="VEM33_00320"
     CDS             2645..>2907
                     /locus_tag="VEM33_00320"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HYS50307.1"
                     /translation="MQSSLRRHLTYWLIGPLLLLVAAGSFVSYRIALSAANKAYDSAL
                     LDPVLAIASHLRHTGGRLELDLPSIAIEALRIDTEDRVYYQVLG"
BASE COUNT          509 a          941 c          904 g          553 t
ORIGIN      
        1 tcccccgtcg ttctgcccgg gcaagtcccc tgagagcggc cccccggcac agccgcagag
       61 caccgcagcg caggcgaagc cagtcgcagc gtgccccgac cgattcaaga gcccgtcccc
      121 tcgaggcgca ccggccttcg ggtgcggtca tggacctttc cggccagctg tccacacgct
      181 gcgtcgattt cgtcacctcg cgtcctgcgc gtggtcgtga tgatccccga tgcgctgagc
      241 agctcgccga accgttcgat cacccggcgc ggggagcgca caaagcccga gctcgggaac
      301 gggttgaacg gaatcaggtt gatcttgcac ggcacccgcg aaaccaattc tacgagctgg
      361 cgtgcgtgct cctcgctatc gttgacgcct tcgagcatga cgtattcgaa ggtgatgaag
      421 tcgcgcggcg cgcgctcgag gtatcgccga cacgcggcca gcaactcggc gagcgggtat
      481 ttgcggttga tcggcatgag cgtgtcgcgc agcgcgtcgt tcggcgcatg caacgacacc
      541 gcgagcgcca ccgggcattc cgccgccagg cggtcgatca tcggcacgat cccggacgtg
      601 gagagcgtca cgcggcgtcg cgaaagcccg tacgcgtcct cgtcgatcat cagcttgagc
      661 gccgcgacgg tgttgtcgaa gttgagcaga ggctcaccca tccccatcag cacgacgttg
      721 ctgatgacgc gcccgccccc gggcgtcgcg cccagctcgc ggttggcgag ccagagctga
      781 ccgatgatct cggacaccga aagattgcgg ttgaatccct gcctgccggt cgagcagaac
      841 tggcaggcaa gggtgcagcc cgcctgcgtg gagatgcaca aggtgccccg gttggtttcg
      901 ggtatgaaca ccgcctcgac cgcgttgccg ctgcccacat cgagcagcca cttgcgagtt
      961 ccgtcccggg ccgagctgtc gctcacgatt cgcggcggcg agacgcacgc ggcccggagg
     1021 agcttttcgc gcaagggctt tgcgatgtcg ctcatccgcg caaaatccga ttcgccgaga
     1081 cggtgcatcc agcgcttcac ctgtcgcgca cggaacggct tctcttcgag ccctgcgacg
     1141 aaggcggcga gcccttcgga gtccagatcg aggagattga cgctcattta acgggaaaag
     1201 atctcggaag cggaaaaaaa gtatgcgatc tcgttgcggg cggtctcggg cccgtcggaa
     1261 ccgtgcaccg cgttcgcatc gatgctctcg gcaaaatcgg cgcggatcgt tccctttttc
     1321 gccttcttcg gatcggtcgc gcccatcagg tcgcggttct tcgcaatggc gtcctcgccc
     1381 tccaggacct gcaccatgat cggcccggag atcatgaatc tgaccagatc gcggaaaaac
     1441 ggacgctccc gatgcaccgc gtagaagcct tcggcttccc tctgcgacag atagcgcatg
     1501 cgcgccgcca cgattttcag gccggcctgc tcgaacttag agtagatttg tccgatggcg
     1561 tttttcgcca ccgcatccgg tttgacgatg gacaaggttc gctctacagc catgcaagat
     1621 ctccggttgg aactctggaa gcgacacaag aaacccgcct aacgttttat tttatcaggc
     1681 ttttgccgga gtgtcgcgcg cggacaagac ccggtacggc ggttcgcctg ccggcggctg
     1741 aaggcagtcc gggaccggcg agaacgcgtt catcatgtca gcgttccgta agtctagcat
     1801 cagcttattt caaggctgac cgcgtagagt ttcatccgta gtctctttcc tcctctgtgg
     1861 cgtgggagcg cgctgcgctc ccacgcactt ttttgcccgg atgtccgtgt gtgcccagct
     1921 cgagaacccg gagcccgtcc gcgtgctatc ctgaacttcg aaccggttca accacttgtc
     1981 atgcgagttc tgatcgtcga agacgacaat gcattggcgg acgggctgat gcgaacgctg
     2041 agacagtcgg ggtacgccgt ggatcacgcg ggatcgggcg agcttgcatt gcgagcctgc
     2101 gcggaggaac actacgacct cgtcgtgctc gatgtcagcc ttccgggcat cgacgggttc
     2161 gaggttctgc gccagctgcg ccgcgacaac cactccggca gcattctgat cctgacagcc
     2221 cacgatgccg aggcggatcg cgtgcgcggc ctggacctcg gggccgacga ctacgtcgcc
     2281 aaacctttct cgcttccgga actcgaagcg cgagtgaggg cgcttatccg ccgcagccag
     2341 gcggtaaaga gtcccaagct gcgcttcggc cgcctcatcg tcgataccgt ggcgcggcgg
     2401 gcgagcatcg gacgggacct gctcgacctc accccgcgcg agtgggcggt gctcgaatac
     2461 ctgctcatgc gggtcgggca ggtggtcagc aaggagcaca tgctgcaggc gatgtgcagc
     2521 tgggacgact ccctgaccca caacgccatc gaggtgtata tctcccggct gcgctcgaaa
     2581 ctccacgatg cgggaatcag gattcgcacc gtgcgtggat tcggctatat ggtcgaagac
     2641 ccggatgcag tctagccttc gccggcatct cacctactgg ttgatcggcc cgttgctgtt
     2701 gctggttgca gcgggaagct tcgtttccta tcgcatcgcg ctcagtgccg cgaacaaggc
     2761 gtacgacagc gcgttgctcg acccggtgct cgcgattgcc agccatcttc gccataccgg
     2821 cggccggctc gaactcgacc tgccttcgat tgcgatcgag gcgctgcgca ttgataccga
     2881 ggaccgcgtc tactatcagg tcctggg
//
LOCUS       DASPZD010000019         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1070730, whole genome shotgun sequence.
ACCESSION   DASPZD010000019 DASPZD010000000
VERSION     DASPZD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070730"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VEM33_00325"
     CDS             <1..220
                     /locus_tag="VEM33_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HYS50308.1"
                     /translation="LFLRRLTAPLALGREIAVGDVEGYVHFLSRESGAFVGRVATDSS
                     PIRSLLVRLDHGLLVQTQDGNLFALSTE"
     gene            253..1578
                     /gene="der"
                     /locus_tag="VEM33_00330"
     CDS             253..1578
                     /gene="der"
                     /locus_tag="VEM33_00330"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HYS50309.1"
                     /translation="MKPTLVIVGRPNVGKSTLFNRLTRTRDALVADVPGLTRDRHYGV
                     GRLGDRPYLAVDTGGLEPEATGVFVEMARQVRTAIAEADAIVLVTDAREGLTARDRAI
                     GEELRRLGRRVFVAVNKAEGMNTELATLEFHEMGLGAPYAISAEHGAGVHDLVEAALA
                     DFPVEQESGEPDSAVRIAIVGRPNAGKSTLVNALVGEERVVTFEEPGTTRDAIDVELV
                     VQGRRYVLVDTAGLRRRGKVFESVEKFSVVKTLQAIDRANVAVLVLDAQGGISDQDAH
                     IAGFILERGRAVVVAVNKWDGMDSYARDMTKRAIARKLKFLSFARFHYISAREGRGLA
                     KLVDSVGAAYKAAMAKLPTPRLTRALIAAVAKQAPPRAGLSRPKLRYAHQGGSNPPIV
                     VVHGTALNGVSDGYRRYLEADFRRVFRLEGTPLRIEFRSGRNPYVDRRV"
     gene            1681..1923
                     /gene="hfq"
                     /locus_tag="VEM33_00335"
     CDS             1681..1923
                     /gene="hfq"
                     /locus_tag="VEM33_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HYS50310.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLKNTVTQMVYKHAISTVVPSRAVNLSADHSSPES"
     gene            1956..3122
                     /gene="hflX"
                     /locus_tag="VEM33_00340"
     CDS             1956..3122
                     /gene="hflX"
                     /locus_tag="VEM33_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYS50311.1"
                     /translation="MAERSSERNAAVLVGLDFGAGGYAEGIEELGRLAASAGIRTRAL
                     VRGRRDRPDPAHFAGTGKVEEIKRTLQEQGASLAIFNHDLSPAQERNLEKALGCPVVD
                     RTRLILNIFAQRAKSHEGKLQVELAQLQHAATRLVRGWTHLERQRGGKGFLGGMGETQ
                     LEVDRRLIANRVKILKDKLGKLGRRREVQRRARARGRAFSVSLVGYTNAGKSTLFNTL
                     THAGALAEDRLFATLDTTTRRLYLPEAGNVAISDTVGFIRDLPHTLVAAFRATLEEAI
                     HADLLLHVVDAASDAREAQILEVDKVLTEIGAGAVPRLLVWNKIDRNGLRPGVDRNEY
                     DRICRVRLSAKTGDGVEGLRQALVEATASRTSPASPPKSTKSTGTQKLGQAATT"
     gene            3130..>3313
                     /locus_tag="VEM33_00345"
     CDS             3130..>3313
                     /locus_tag="VEM33_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HYS50312.1"
                     /translation="MSLNDPQWGKRGGGNEGPPDLDEMWRRFNQKLNGLFGRRLGGGE
                     GPGEPPTVRQLGGGAGL"
BASE COUNT          624 a         1069 c         1088 g          532 t
ORIGIN      
        1 cctgttcctg aggcgcctga ccgcgcctct ggccctgggg cgagagatcg ccgtcggcga
       61 cgtcgaaggc tacgtgcatt ttctttcgcg cgaaagcggc gccttcgtcg gccgtgtcgc
      121 caccgacagc agcccgatca gatcgttgct cgtgcggctc gaccacgggc tcctggtgca
      181 gactcaggac gggaatctgt tcgccctgag cacggagtag ctaggatcgg acccagccac
      241 ggtcctctcg tcatgaagcc aaccctcgtc atcgtcggca ggcccaacgt cggcaagtcc
      301 accctgttca accggctcac gcgcacccgg gatgcactgg tcgccgatgt cccgggtctg
      361 acgcgagacc gccactacgg agtcggacgc ctcggagatc ggccgtacct cgcggtcgat
      421 accggcgggc tggaacccga agcgacgggg gtgttcgtcg agatggccag acaggtgaga
      481 accgcaatcg ccgaagcgga cgccatcgtc ctcgtgaccg acgcccgcga gggcctgacg
      541 gcgcgggacc gagcgattgg agaggagctg cggcggctcg gccggcgcgt gttcgtcgcg
      601 gtcaacaagg ccgagggcat gaatacggag ctcgcgacgc tcgaattcca cgaaatgggg
      661 ctcggtgcgc catacgcgat ttccgccgag catggcgcag gagtgcacga cctggtcgag
      721 gccgcgcttg ccgatttccc cgtcgaacag gagagcggcg aacccgacag cgcggtccgg
      781 atcgccatcg tgggtcgtcc caacgccggg aaatccacgc tcgtcaacgc gctggtcggc
      841 gaggagcgcg tggtgacgtt cgaagagccg ggcactacgc gcgatgcgat cgacgtcgag
      901 ctcgtggtgc aaggccgacg ctatgtcctc gtcgacaccg ccggattgag acgccgcggg
      961 aaggtgttcg aaagcgtcga gaagttttcg gtcgtgaaga ccttgcaggc catcgatcgg
     1021 gccaacgtcg cagtcctcgt cctcgatgcc caaggcggga tctcggacca ggacgcgcac
     1081 atcgccgggt tcatcctcga gcgcgggcga gccgtggtcg tggcggtgaa caaatgggac
     1141 ggcatggaca gctacgcgcg cgacatgacc aagcgcgcca tcgctcgcaa gctgaagttt
     1201 ctctcgtttg cacgcttcca ctacatctct gcacgcgaag gccgcggcct cgcgaagctc
     1261 gtcgattcgg tgggtgccgc ctacaaggcc gcaatggcga agctgcccac cccgagactc
     1321 acccgcgccc tcatcgccgc cgtggcgaag caggccccgc cgcgcgcggg gctctcgcgc
     1381 cccaagctcc gctatgcgca tcaaggcggg agcaaccctc cgatcgtggt cgtgcacggg
     1441 actgcgttga acggcgtgtc cgacggctac cgccgttatc tcgaggccga cttccgcagg
     1501 gtgtttcggc tggaagggac gcccctccgg atcgaatttc gcagcggcag gaacccttac
     1561 gtcgacaggc gcgtctaaga ggcggcgccg agggcttcaa agccgccgct ttagtacatt
     1621 ggcattttcc gcaaaaccat ttacagtaga ccttcaaata atatgactcc ggggtaaacc
     1681 atgagcaaca aaggacaatt actacaagat ccgttcctga ataccctgcg caaggagcac
     1741 gtgccggtat ccatctatct cgtcaacggt atcaagctcc agggacagat cgagtccttc
     1801 gaccagtacg tcgtgctcct caagaacacc gtgacgcaga tggtgtacaa gcacgccatc
     1861 tccaccgtcg tgccttcgcg tgcggtcaat ctgtcggccg atcattcctc tcccgagagc
     1921 tgatacggcc gcgtcatcga ctccgatccg gcgcgatggc tgagcgttcg tcggagcgca
     1981 acgccgccgt gctcgtgggc ctggatttcg gtgcgggcgg ctacgccgaa ggcattgaag
     2041 aactgggccg tctcgcagca agcgcgggaa ttcgaacgcg ggctctggtg cgggggcgcc
     2101 gcgaccgacc cgaccccgcc catttcgcgg gtaccggaaa ggtcgaggag atcaaacgaa
     2161 ccctgcagga gcagggcgcc tcgctcgcga ttttcaatca cgacttgtcc cccgcgcagg
     2221 agcgcaatct cgagaaggcg ctcggctgcc cggtcgtcga ccgcacccgg ctgatcctca
     2281 acatcttcgc ccagcgcgcc aagagtcacg aaggcaagct ccaggtggag cttgcacaac
     2341 tccagcacgc ggcgacgcgg ctggtgcgcg gctggacgca cctggagcgg caacgcggcg
     2401 gcaagggctt tctcggcgga atgggcgaga cgcagctcga ggttgacagg cgcttgatcg
     2461 cgaaccgggt caagatcctc aaggacaagc tcggcaagct cggccgtcgg cgcgaggtcc
     2521 agcggcgcgc gcgggctcgc ggcagggctt tttcggtctc gctcgtgggt tacaccaacg
     2581 cgggcaagtc gacgctattc aacacactga ctcacgcggg ggcgcttgcc gaggatcggc
     2641 tcttcgcaac gctcgatacg accacgcgca ggttgtacct gcccgaggca gggaacgtcg
     2701 cgatctccga cacggtcggg ttcatacgcg atcttccgca tacgctcgtc gcggccttcc
     2761 gggcgacgct cgaagaagcc atccacgccg acctgctgct gcacgtggtc gatgccgcga
     2821 gcgatgcacg cgaggcgcag atcctggaag tggacaaggt gctcaccgaa atcggcgcgg
     2881 gcgccgttcc gcggctcttg gtgtggaaca agatcgaccg caacggcctt cgcccgggag
     2941 tcgaccgtaa cgaatatgat agaatctgcc gcgttcgtct aagcgcaaaa acgggcgatg
     3001 gcgtggaggg cctgcgtcag gccctggtcg aggcaactgc ctcgaggaca tctccggcga
     3061 gcccgccgaa atcgacaaaa tcgacaggta cgcagaaact gggccaagcg gccacaacgt
     3121 aggattctca tgtcattgaa cgacccgcaa tggggcaagc gaggcggagg caacgagggg
     3181 ccgcccgatc tcgacgagat gtggcgcagg ttcaaccaga agctgaacgg cctgttcggc
     3241 cggcgccttg gcggagggga gggtcccggc gaaccgccga ccgtcaggca gctcggcggc
     3301 ggagcgggcc tgc
//
LOCUS       DASPZD010000020         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1193678, whole genome shotgun sequence.
ACCESSION   DASPZD010000020 DASPZD010000000
VERSION     DASPZD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193678"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VEM33_00350"
     CDS             complement(<1..925)
                     /locus_tag="VEM33_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYS50313.1"
                     /translation="MADLAVPITLAPAPADDLWSVDERSLDLFAKQLDAPSAPRAWYE
                     LRREAEHVALVPGFDRLITLGGHAIKELPHQIDVAQRVLRQMGGRAILADEVGLGKTI
                     EASIIYRELAIRGLARRTLILTPASLVGQWQGELEEKFFQHFDTPTDPDDWQRVTRAI
                     VSHDRARSRRHAEEILRHHWDLVIVDEAHKIKSHRGATYQFIEKIERDFILLLTATPL
                     QNDMRELYNLVTLLRPGQLGTWEEFRAAHLVGGDPRQPKDPEALRALTHAVMIRTRRA
                     SVVDDLDLPPRRPRHPAVKLTKAEADLYERTT"
     gene            1351..>2128
                     /locus_tag="VEM33_00355"
     CDS             1351..>2128
                     /locus_tag="VEM33_00355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYS50314.1"
                     /translation="MTASIGTEFLALQELLAGRYSIERELGRGGMGIVLLARDVALDR
                     PVAIKLLPPHLATLPDERDRFQQEARTAAGLAHPNIVPIHLVEAHGDLVFFVMGFVDG
                     ETLRDRVERAGPLPPRLATKLLQEVAWALGAAHQRGVIHRDVKPDNIMIERATERAVV
                     TDFGIALGSRAGKAESPGGAITGTARYMSPEQACGEPVDARSDLYSLGATFFYALTGR
                     APFEAANAPAIITKHVYEIAPRIQQLRPEVPAKLDGVVDRL"
BASE COUNT          347 a          700 c          726 g          355 t
ORIGIN      
        1 cggtggtgcg ctcgtagagg tcggcctccg ccttggtcag cttgaccgcg ggatgccggg
       61 gacggcgggg cggcaggtcc agatcgtcga ccacgctcgc gcggcgcgtg cggatcatga
      121 ccgcgtgggt gagcgcgcgc agcgcctcgg ggtccttggg ctggcgcgga tcgcccccca
      181 ccaggtgcgc ggcgcggaat tcctcccagg tgccgagctg cccggggcgg agcagggtga
      241 cgaggttgta cagctcgcgc atgtcgttct gcagtggtgt cgccgtgagc agcaggatga
      301 agtcgcgctc gatcttctcg atgaactggt acgtcgcgcc gcggtggctc ttgatcttgt
      361 gcgcttcgtc cacgatcacg aggtcccaat gatgccgcag aatctcctcg gcgtgccggc
      421 gggagcgcgc gcggtcgtgc gacacgatcg cgcgggtgac gcgctgccag tcgtcgggat
      481 cggtcggggt gtcgaagtgc tgaaagaact tctcctccag ctcgccctgc cactgcccca
      541 ccagcgacgc cggggtgagg atgagcgtgc ggcgggccag gccgcgaatc gcgagctccc
      601 ggtagatgat gctcgcctcg atcgtcttgc cgagcccgac ttcgtcggcg aggatcgcgc
      661 ggccgcccat ctgccgcagc acccgctggg cgacgtcgat ctggtgcggc agctctttga
      721 tcgcgtgccc gcccagcgtg atgagccggt cgaagcccgg gacgagcgcg acgtgctccg
      781 cctcgcgccg cagctcgtac cacgcgcgcg gtgccgacgg cgcgtcgagc tgcttggcaa
      841 agaggtctaa cgagcgttcg tcgaccgacc agagatcgtc ggcaggtgcg ggggcgagcg
      901 taatcgggac agcgagatcg gccattttgc agcagcggga agaggggccg caatattcaa
      961 ttcgtggctc aaccgcgcca gactccacac cctgaagggt gtgctcggct gacactgtcc
     1021 ttaaggacaa tgcttgccga gcgcaggctt tagcctgtga aaacacgaaa gccggcgagc
     1081 tttcgctccc cggctttctc acgtagcgaa actgactacg cagtgacctc gcaagtatgt
     1141 caaacgtcgg caaccaacac tgccgagtca ataggaaaga gcgagggtac tggtgatggt
     1201 tgtggtattg gcgattgaaa ctggccactc agcactgcag gccactgctg cgtctgctgc
     1261 gtctgtggcg tcttctgatt tcggggtgct gatcgcctac agagttcctt tcgaaacgca
     1321 gcgcgcctcc actcgtagga cgagtcaccc atgaccgcgt cgatcggcac cgaattcctc
     1381 gccctccagg agctgctcgc cggccgctat tcgatcgagc gcgagctcgg ccgcggcggt
     1441 atgggcatcg tgttgctcgc ccgtgatgtc gcgctcgacc gccccgtcgc gatcaagctg
     1501 ctcccgccac acctcgctac gctccccgac gaacgggacc gcttccagca ggaggcgcgg
     1561 accgccgcgg gcctagcgca tccgaacatc gtgccgattc acctcgtcga agcacacggt
     1621 gacctcgtgt tcttcgtgat gggcttcgtg gacggcgaga cactgcgcga tcgcgtggaa
     1681 cgggccggcc cgttgccgcc gcgcctcgcg acgaagctgc tgcaggaagt ggcgtgggcg
     1741 ctgggcgccg cacatcaacg cggcgtgatt caccgcgacg tgaagcccga caacatcatg
     1801 atcgaacgcg cgaccgagcg cgcggtcgtc accgatttcg ggatcgcgct cggaagccga
     1861 gcggggaagg cggaaagccc gggcggcgcg atcaccggga ccgcgcgcta catgagcccc
     1921 gagcaggcgt gcggcgagcc ggtggacgcg cgcagcgacc tctactccct gggcgcgacg
     1981 ttcttctacg cgctcaccgg ccgggcacca ttcgaggctg caaacgcgcc ggcgatcatc
     2041 acgaaacacg tctacgagat cgcgccacgg atccagcagc tccggcccga ggtgcccgcc
     2101 aagcttgacg gcgtggtcga ccggctgc
//
LOCUS       DASPZD010000021         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_346189, whole genome shotgun sequence.
ACCESSION   DASPZD010000021 DASPZD010000000
VERSION     DASPZD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346189"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VEM33_00360"
     CDS             complement(<1..528)
                     /locus_tag="VEM33_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50315.1"
                     /translation="MKRLALLAAAMLLPVCVSAQTYPTRAIRIVVAFPAGGGSDLAAR
                     VVAQKLSESLGQPVVVENRVGANGGVGAEAVARSAPDGYTLVMGSNANITTNPHLMAL
                     AYDPMKDLAPVAMLTVNPLLLFVNPAVVPVASFREFLDYVRARDGKVDYASAGNGSPA
                     HLSGELLKLTAGIRMV"
     gene            complement(525..1373)
                     /locus_tag="VEM33_00365"
     CDS             complement(525..1373)
                     /locus_tag="VEM33_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50316.1"
                     /translation="MNSAAGERREIEYAVHGGVSLKGHLYAPKGPGKFPIVIAVHGGG
                     WRLANLDNYRILGAWLAERGYATLAVTHRLSKPAEKAYPEAVQDVRAAVQFARGKAAE
                     LGIDPERIALMGDSSGGHLAALVALAGEHALFREGNAADPHGRVSTAVKVCVPIYSVL
                     DLARQWRHDQVSRPRDQIVEKFLGASLIDDRRIYFDASPLSYVSAKNNGTAFLLAWGT
                     ADDVVDHEEQSEAFLEALKQAGHYVRSVVLAGAPHFWVGDPLDEPGSHSGFFAQRLLR
                     FLQARL"
     gene            1435..2460
                     /locus_tag="VEM33_00370"
     CDS             1435..2460
                     /locus_tag="VEM33_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS50317.1"
                     /translation="MKPAALAAGLVALTCASALAQSFSELALDGRPERFQRLVAGAKK
                     EGTLTLYTSIPEKDMAVLTADFERRHGVKVNVWRASSVKVLQRAAAEARANRWDFDAA
                     AISSPEMEAMHREVLLQEVRSRHHADLAPDAMPSHRAWVPQFLNVFVQAYNTSLVRKE
                     DLPKSYAELADPRWKGRLGVEASDSEWYCGVLRHAGAESGAKLFRDIVATAGWSVRSG
                     HTLLANLVASGEVPLALTVYSYRIEQLKSAGAPVEWFGIDPVIGRANGAGVSRRPPHP
                     SAALLFYEYLISDAQPLMVKMNYLAADTRIASSLRGVKVRFIDPRTPLEELDRCAKAY
                     DELNGVR"
     gene            complement(2469..3164)
                     /locus_tag="VEM33_00375"
     CDS             complement(2469..3164)
                     /locus_tag="VEM33_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYS50318.1"
                     /translation="MNSVRLILIASAALSGCVAGVPQREEASKVPTMTALPKDIHPES
                     RSRLPLPKREALDEQGKRVYDSVLDPKRPTLAGFQGPAGIWLHSPRVGEPFREMNRIL
                     RNEVPLEPALRELAILVTARELDSQFEWTAHEPVALKEGLDPKILEAVKFRKAVSGAP
                     EKEAAIVAFGRELFRDRKVRSETYAEMVRVFGQTAVVNITALMANYAFTAVMLTAFDQ
                     QLHEGRKPLLPLP"
     gene            complement(3181..>3865)
                     /locus_tag="VEM33_00380"
     CDS             complement(3181..>3865)
                     /locus_tag="VEM33_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50319.1"
                     /translation="VAIVQSMVKDLAFDPRRDLAPVALVGVIPNVLVAGPRTPAKTLA
                     ELVELARRNPGKLNYSSTGAGTSVHLSAELLKYYAGADIVHVPYRGAAAAMTALLSGD
                     VDIMVDSVPPSLPHIRAGKVRALAVTSARRVPQLPEVPTMIESGYPDFEINGWSGVVT
                     TAGTPGAIIARLESEIGRALSQPQVAAAYENAGLTVRFMGAAEFGKFWDAEIAKFALA
                     IKHSGAVKE"
BASE COUNT          634 a         1315 c         1283 g          633 t
ORIGIN      
        1 caccattctg attcccgcgg tgagcttcag cagctcgccg gacaggtgcg cgggcgagcc
       61 gttgcccgcc gaggcgtagt cgaccttccc gtcccgcgcc ctgacgtagt cgaggaattc
      121 ccggaaagag gcgaccggca ccaccgccgg attgacgaag agcagcaagg gattcaccgt
      181 gagcatcgcc accggcgcga ggtccttcat cggatcgtag gcgagcgcca tcaagtgcgg
      241 gttggtggtg atgttggcgt tgctgcccat caccagcgtg tacccgtcgg gggccgagcg
      301 cgccactgcc tccgcgccga caccgccgtt cgcgccgacg cggttttcca cgacgaccgg
      361 ctggccgagg ctctccgaga gcttctgcgc gaccacgcgc gccgcgaggt ccgagccgcc
      421 gcccgcggga aacgccacca cgatcctgat cgcgcgagtc ggataggttt gcgccgatac
      481 gcagacgggc aatagcatag ccgccgcgag cagagcgagt cgcttcatag tctcgcctga
      541 agaaagcgca gcaagcgctg cgcgaagaag ccggaatggc tccccggctc gtccagcgga
      601 tcgcctaccc agaagtgcgg cgcgccggca agcaccaccg agcgcacgta atgcccggcc
      661 tgcttcagcg cctcgaggaa ggcttcgctc tgctcctcgt ggtcgaccac gtcgtccgcg
      721 gtgccccagg cgaggagaaa ggccgtgccg ttgtttttgg ccgaaacgta ggaaagcggc
      781 gaagcgtcga agtagatgcg ccggtcgtcg atgaggctcg cgccgagaaa cttctcgacg
      841 atctggtcgc gcggccgcga gacctggtcg tggcgccact ggcgcgcgag atccaggacc
      901 gagtagatcg ggacgcagac cttcactgcg gtcgacacgc ggccgtgcgg atcggcggcg
      961 ttgccttcgc ggaacagcgc gtgctctccg gcgagcgcga cgagcgcggc gaggtgcccg
     1021 cccgaggaat cgcccatgag cgcgatgcgc tcgggatcga ttccgagttc cgcggccttt
     1081 cccctcgcga actgcaccgc ggcgcgcacg tcctgcacgg cctcgggata ggccttctcc
     1141 gcgggcttgg acagacggtg cgtcacggcg agcgtggcgt agccgcgctc ggcgagccag
     1201 gccccgagga tgcggtagtt gtcgagattg gcgaggcgcc accctccgcc gtgcaccgcg
     1261 atcacgatgg gaaatttgcc cggacctttc ggcgcataca ggtgcccctt cagcgatacg
     1321 ccgccgtgaa cggcatactc gatttcgcgg cgctcgccgg ctgcactgtt cattttcctc
     1381 tccctgtctt ccgcggcggc cagaccatta ctataccgtt acaatgagcc ggcaatgaag
     1441 cccgccgcgc tcgcagccgg tctcgtcgcg cttacctgcg cgagcgcgct ggcccagtcc
     1501 ttctccgagc tcgctctcga cgggcggccc gagcgcttcc agcgcctcgt cgcaggggcg
     1561 aagaaggaag gcacgctcac tctctacacc tcgatcccgg agaaggacat ggcggtgctg
     1621 accgccgatt tcgaaagacg ccacggcgtc aaggtgaacg tctggcgcgc ttcctcggtc
     1681 aaggtcctgc agcgcgccgc agccgaagcg cgcgcgaacc gctgggattt cgatgcggct
     1741 gcgatctcgt cgcccgagat ggaggcgatg caccgggagg tgctgctgca ggaggtgcgc
     1801 tcgcgccatc acgcggacct cgcgcccgac gcgatgcctt cgcaccgtgc ctgggtgccg
     1861 caattcctga acgtattcgt ccaggcgtac aacacctccc tcgtgaggaa ggaggatctg
     1921 ccgaagtcct atgccgaact cgccgaccct aggtggaagg gccggctggg cgtggaggcc
     1981 tccgacagcg agtggtactg cggcgtgctg cggcacgcgg gcgcggaaag cggcgcgaag
     2041 ctgtttcgcg acatcgtcgc gacggccggc tggtcggtga ggagcggcca tacgctgctc
     2101 gcgaacctgg tcgcctcggg cgaggtgccg ctcgccctga ccgtctacag ctaccgcatc
     2161 gagcagctga agagcgcggg cgctccggtc gagtggttcg gaatcgatcc ggtgatcggc
     2221 cgggcgaacg gcgccggcgt ctccaggcgg cctccgcatc ccagcgccgc cctgctcttc
     2281 tacgaatacc tcatcagcga tgcgcagccc ctgatggtga aaatgaacta tctcgcggcg
     2341 gacacccgga tcgcctcatc cctgcgcggc gtgaaagtcc ggttcatcga cccgcgcaca
     2401 ccgctcgagg agctcgaccg ctgcgcaaag gcgtacgacg agttgaacgg cgtccggtag
     2461 cgggaggctt agggaagcgg cagcaggggc ttcctgcctt cgtgcagctg ctggtcgaaa
     2521 gcggtgagca tcaccgcggt gaacgcgtag ttcgccatca gcgccgtgat gttcacgacc
     2581 gcggtctgac cgaagaccct caccatctcc gcgtaggttt cggagcggac ttttctgtcg
     2641 cgaaagagct cgcggccgaa ggcgacgatt gccgcctcct tctccggcgc tcccgagacg
     2701 gccttgcgga acttcactgc ttcgaggatc ttgggatcca ggccctcctt caacgcgacc
     2761 ggctcgtgcg ccgtccactc gaactggctg tcgagctcgc gcgcggtcac gaggatcgca
     2821 agctccctca aggcgggctc gagcggcacc tcgttgcgca ggatgcggtt catctcccgg
     2881 aacggctctc ccaccctggg gctatgcagc cagatgccgg cgggcccctg aaaacccgcg
     2941 agcgtgggac gcttgggatc gagaaccgag tcgtagacgc gctttccctg ctcgtcgagc
     3001 gcctcgcgct tcgggagcgg cagccgggag cgtgattcgg gatggatgtc tttgggcaga
     3061 gcggtcatgg tcggcacctt ggaagcttcc tcgcgctgcg ggacgcctgc gacgcatccc
     3121 gagagcgctg cagaagcgat cagaatcaag cgaactgaat tcatcctctc cctcctccga
     3181 ttactccttc accgcgcctg agtgcttgat cgcgagcgcg aacttcgcga tctccgcgtc
     3241 ccagaacttc ccgaattccg ctgcgcccat aaagcgcacc gtgagtcccg cgttttcgta
     3301 ggccgcggcg acctgcggtt gcgaaagcgc ccttccgatt tccgactcga gccgcgcgat
     3361 gatcgcgccc ggcgttccgg cagtggtcac gacccccgac cagccgttga tctcgaagtc
     3421 cggatatccc gactcgatca tggtcggcac ttcggggagc tgcggcacgc gccgcgcgct
     3481 cgtcaccgcg agcgcgcgca ctttccccgc gcggatatgc ggcagcgagg gcggcaccga
     3541 atcgaccatg atgtccacgt cgcctgagag gagcgccgtc atcgccgccg ccgcgccgcg
     3601 gtagggcacg tgcacgatat cggcgccggc ataatacttg agcagctccg cggaaagatg
     3661 cacggaggtg cccgcgccgg tcgacgagta attcagcttg ccgggattgc ggcgcgcgag
     3721 ctcgaccagc tcggcgagcg tcttcgcggg cgtgcgcggc ccggcgacga gcacgttcgg
     3781 tatcacgccg acgagcgcga ccggcgcgag gtcgcggcgc ggatcgaagg cgaggtcctt
     3841 caccatcgac tgcacgatcg ccacc
//
LOCUS       DASPZD010000022         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_345610, whole genome shotgun sequence.
ACCESSION   DASPZD010000022 DASPZD010000000
VERSION     DASPZD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345610"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1059)
                     /gene="xerD"
                     /locus_tag="VEM33_00385"
     CDS             complement(139..1059)
                     /gene="xerD"
                     /locus_tag="VEM33_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYS50320.1"
                     /translation="MNADNAALLDEFCDAVWLEDGLAKNTLESYRCDLSLFADWHERR
                     GRASLARATEADLAEYFAWRYSRRSPGKKHGIRSSTQARLHSSLKRFYRFLARGQRIE
                     TDPTLKLDPPKKPPRFPKTLSEVDVEALLAQPDPSTPLGLRDRAMLEVLYASGLRVSE
                     LVRLKLAELSFDMGVVRVFGKGSKERLVPLGEEALAWLARYLKEGRPVLLRKRGSDYV
                     FVTGRGGPMTRQAFWHLIKKRAGAALPGKSLSPHTLRHAFATHLLNHGADLRVVQMLL
                     GHADISTTQIYTHVARERLKKLHKDHHPRG"
     gene            complement(1064..1546)
                     /locus_tag="VEM33_00390"
     CDS             complement(1064..1546)
                     /locus_tag="VEM33_00390"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYS50321.1"
                     /translation="MPDVSGSFQAKLPAPFGLLGIRTAGGRLSEIVFLKPGGAALAPR
                     DGVSERTCRQLQSYFDDPEFRFDVPLAAQGTPFQRRVWRKIAAIGAGRTRSYGELARE
                     LRSAPRAVGQACGANPVPLVVPCHRVLAASGIGGFAHHEAGFHLAIKRWLLAHEGAAR
                     "
     gene            complement(1656..3689)
                     /locus_tag="VEM33_00395"
     CDS             complement(1656..3689)
                     /locus_tag="VEM33_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HYS50322.1"
                     /translation="MSQIAGPAAGEPAIDAEALKKAEEYIEAEEGASNKLRGWLGVFV
                     SVVAVVMSVFHLYTAYAIVPTQVLRPVHVGFVLFLSYLLFPVAKRYRHRIMWWDWAAA
                     FVAVAVIAYILQGGDEIWDRNTSPDNWDIAFGIALILLILEGMRRASGWVMPVVVTSF
                     VLYALVGEQLPGSWAHRSYEVGRLVGHLYMTLEGIFGVAVDVSSSLIILFTIYGAFLQ
                     FSGAGKFFIDFSFSAMGGKSTSAGRTVVLASFLLGGPSGSGVATTVTLGSVAYPMLVR
                     AGYGKDAAGGLLAAGGLGAIISPPVLGAAAFLIAEFLKISYLDVLLMATIPTLLYYFA
                     LFLMVEIDARKFGMSTVKYVQVESLWSLTKNYWFHFLSLVSIVVFMLMGYSPVLSVFW
                     ATVFSFATSFLHRDCALFSYDLFRGREPLVKGIFGSKFFKALEAGSTGVLNVATTCAG
                     AGIIVGVVTLTGLGLKFSSIIIEYAGGSLLLTAIYTSLIVWVVGLAVPVTASYIICAV
                     IAAPAMIKLGVPDFAAHMFIFYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQSWKY
                     TLPAFLVPFMFVLDPSGVGLLLTGSIKTLGNADWGAIAVVTVTAALGIAALAGGVQGW
                     LFGRTAWYEKWMLIAAGLALVYPTTTADVIGFGLFGLVVVQQLLRRRAVRSTT"
     gene            complement(3750..>4966)
                     /locus_tag="VEM33_00400"
     CDS             complement(3750..>4966)
                     /locus_tag="VEM33_00400"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50323.1"
                     /translation="TPNAEVGNMIGGLLKALSITGCFIVLAGCAAAPRAPLPAHLLWQ
                     DKAFDYDAALVSVSKRDLFQLDPELLSKLQDPRIQNSSTQYRARHLVSLLFGRETKDF
                     PYSGGHSTIAAETWRRKSGDCLSLTVLAYSLANALGMSVQMQEVRVPVVFDRRGNVES
                     VNRHVNVLIRSVGQLYLKDGSMRSGDVIIDFEPQIASRREGLALSGDGILARFYNNVA
                     VEYLAQGDLTLAYAHFKAAILADAGYSATYANLAQLYKRRGLLQSAEQLLLYAIALND
                     DADIALRSLHQLLVSQGRESEALKYAEILRARQEKDPYYWLGVGLDHLQRGDYPKAVD
                     ALEHAQGLTTGFQEVHRYLAIAYWRAGKQARAKNQLSVLASLVPDDSALPALRRKIDK
                     APDDRRLVLPAE"
BASE COUNT         1017 a         1560 c         1569 g          820 t
ORIGIN      
        1 gagcggatcg cgagtctcct gatggacgac ttcaagtcgc cgcacgtcaa ggtcagcgtc
       61 gccaagctcg gggcgctgcg cgaatcgcag cgcgtgggcg tcacgataga aagaccgaaa
      121 tcgcgagcgt agtcctgatc acccgcgtgg gtgatggtcc ttgtgcagct tcttcagccg
      181 ctcgcgcgca acgtgggtgt agatctgtgt ggtcgaaata tcggcgtggc cgagcaacat
      241 ctgcacgacg cgcagatcgg cgccgtggtt gagcagatgc gtggcgaagg cgtggcgcag
      301 cgtgtggggc gagagcgatt tgccggggag cgctgccccg gcgcgcttct tgatcagatg
      361 ccagaacgcc tgccgcgtca tggggccacc gcgccccgtg acgaacacgt agtcgctgcc
      421 ccgtttcctc aagagcacgg ggcggccttc cttcaaatag cgtgcgagcc aggcgagtgc
      481 ttcctccccg agaggcacga ggcgttcctt cgagcccttg ccgaaaacgc gcaccacgcc
      541 catgtcgaag ctcaattcgg cgagcttcag gcgaacgagc tccgatacgc gcagcccgct
      601 cgcatagagc acttccagca ttgcacggtc gcggagcccg agcggcgtgc tcgggtcagg
      661 ctgcgcgagc agcgcttcga catcgacctc cgagagcgtc tttggaaagc gcggcggctt
      721 cttcggggga tcgagcttga gcgtgggatc ggtctcgatg cgctgcccgc gggcgagaaa
      781 ccggtagaag cgttttaagc tcgagtgcag ccgcgcctgg gtgctcgagc ggataccgtg
      841 cttcttcccg ggagagcgcc gcgaataacg ccaggcgaaa tactccgcga gatcggcttc
      901 ggttgcgcgc gcgagcgacg cacgcccgcg gcgttcgtgc caatcggcga acaggctaag
      961 atcgcagcgg taggattcga gcgtgttttt cgcaaggccg tcctcgagcc agacggcgtc
     1021 gcagaattcg tccaggagcg cggcattgtc cgcgttcatt ggttcagcga gcggcgccct
     1081 cgtgtgcgag cagccagcgc ttgatggcga gatggaatcc cgcctcgtgg tgggcaaagc
     1141 cgccgattcc gctcgcagcg agcacgcggt gacagggaac cacgaggggg acggggttgg
     1201 cgccacaggc ctggcccacg gcgcgcggtg cggaacgcag ctcgcgcgca agctccccgt
     1261 agcttcgggt gcggcctgcg ccgatcgcgg cgatttttct ccacacgcgg cgctggaacg
     1321 gcgtgccttg cgctgccagc gggacgtcga aacggaactc gggatcgtcg aaatagcttt
     1381 ggagctgccg gcaggtgcgc tcggaaacgc cgtcccgcgg cgccaacgca gcgccacccg
     1441 gcttcaggaa cacgatttcg gagagacgtc ccccggccgt gcggatcccg agcaggccga
     1501 acggcgccgg cagtttcgcc tggaagcttc ccgacacgtc tggcatcgtg cggggattgt
     1561 agcgcgggcc ggggcttaaa actcacggac gcgttttgag cggctttctg gagcctcctg
     1621 agaacctgaa gtcgacgcgt ctgtgggccc atgccctacg tagtcgaacg cacggcccgc
     1681 ctgcgcagca gttgctggac gaccaccagc ccgaacaggc caaagccaat cacgtcggcc
     1741 gtggtcgtcg gatacacgag cgccagcccg gcggcgatca gcatccactt ttcgtaccat
     1801 gcggtgcgcc cgaacagcca gccctgcaca ccgcccgcga gcgcggcgat gccaagcgca
     1861 gcggtgacgg tgacgaccgc tatcgcgccc cagtcggcgt tgccgagcgt tttgatcgaa
     1921 ccggtaagga gcaatcccac gccggaggga tcgagcacga acatgaaggg cacgaggaac
     1981 gcgggcagag tgtatttcca ggactgcagc gtggtcttgt agggatcgcc gcccgtgatc
     2041 gctgcggcgg cgaaaggcga gagcgcggtt ggcggcgaga cttccgagag caccgcgtag
     2101 tagaagatga acatgtgcgc ggcgaagtcc ggcaccccga gcttgatcat ggcaggtgcc
     2161 gcgatcaccg cgcagatgat gtaggacgcg gtcaccggga cggcaagccc cacgacccaa
     2221 acgatgagcg aagtgtagat cgccgtcagc agcagcgagc ccccggcgta ctcgatgatg
     2281 atcgagctga atttgaggcc cagacccgtg agcgtcacta caccgacgat gatgccggcc
     2341 cccgcgcaag tggtcgcgac gttgagcacc ccggtcgagc ccgcctcgag cgccttgaag
     2401 aatttcgacc cgaagattcc ctttaccagg ggctcgcgcc cgcggaacag gtcgtaggag
     2461 aacaaggcgc aatcgcgatg caagaagctc gttgcaaaag agaacaccgt ggcccagaac
     2521 accgacagca cgggcgagta tcccatgagc atgaatacga cgatcgacac cagcgaaagg
     2581 aagtggaacc agtagttttt cgtcaagctc cacagcgatt cgacctgcac gtacttcacc
     2641 gtgctcatgc cgaacttgcg tgcgtcgatc tcgaccatta ggaagagcgc gaagtagtag
     2701 agcaaggtcg ggatcgtcgc catcaagagc acgtcgagat acgagatctt caggaactcg
     2761 gcgatgagaa aggccgccgc accgagcaca ggcggcgaga tgatcgcgcc caaccccccc
     2821 gccgcaagca gcccgcccgc cgcgtccttg ccgtatccag ccctgacgag catcgggtag
     2881 gcgaccgagc ccagcgtcac ggtggtcgcg acgccgctcc ctgacgggcc accgagcaga
     2941 aacgaggcga gcaccacggt gcggccggcg ctggtcgact tgccgcccat ggcggaaaac
     3001 gagaaatcga tgaagaactt gcccgcgccg gagaattgca ggaacgcccc gtagatcgtg
     3061 aagaggatga tgagcgagga agaaacgtcc acggcgacgc cgaaaatccc ttccagcgtc
     3121 atgtacaaat gccccaccag gcggccgact tcgtagcttc gatgcgccca ggaaccgggc
     3181 agctgctcgc cgacgagcgc atagaggacg aaggaggtga ccacgacagg catcacccag
     3241 ccggaggcgc ggcgcattcc ctcgaggatg aggaggatca acgcgatgcc gaaggcgatg
     3301 tcccagttgt ccggcgaagt gttgcggtcc cagatttcgt cgccgccctg gagtatgtac
     3361 gcgatcacgg ctacggcaac gaacgccgcc gcccagtccc accacatgat gcggtggcgg
     3421 tagcgcttcg cgaccggaaa cagcaggtag ctgaggaaca gcacgaagcc gacgtgcacc
     3481 gggcgcagca cttgcgtcgg cacgatcgcg tacgcggtat agaggtggaa caccgacatc
     3541 accaccgcaa cgacgctgac gaagacgccg agccatccgc gcagcttgtt gcttgcgccc
     3601 tcctcggcct cgatgtactc ctcggccttc ttcagcgcct cggcgtcgat tgcaggctca
     3661 ccggccgcag gccccgcgat ctgactcatc ccgcctcctc ggcgcgcatg ctagcacagc
     3721 ccgcccgcgc gggacgctgc ccgccctgct cactcggccg gcaggacaag ccgccgatcg
     3781 tcaggggctt tatcgatttt ccttctcagc gcggggagcg ccgaatcgtc agggaccaat
     3841 gacgccaaca ccgagagttg attcttcgca cgcgcctgct tgccggcccg ccaataggca
     3901 atggccaaat agcggtgcac ttcctggaat ccggtggtca ggccttgcgc atgctcgaga
     3961 gcgtcgacgg ctttaggata atcccctctc tggaggtgat cgagcccgac gccgagccag
     4021 taatagggat ccttctcttg tcttgcccgc agaatctccg cgtatttcaa ggcctctgat
     4081 tcgcgacctt gggataccag cagctgatgc agggaacgca atgcaatgtc cgcgtcatcg
     4141 ttgagtgcaa tggcgtacag caacaattgt tccgcgctct gaaggaggcc tctgcgtttg
     4201 tagagctgcg ccaaattggc ataagtcgcc gaatagcccg cgtccgcaag tatcgccgct
     4261 ttgaagtggg cataagccag agtcagatca ccttgcgcca aatattccac cgcaacgttg
     4321 ttatagaagc gcgcaagaat accgtcgccc gacaaggcca atccctctcg ccgagaggcg
     4381 atttggggct cgaaatcgat gatcacatca cccgatcgca tcgatccgtc tttgaggtac
     4441 agctgtccaa cgctcctgat gagcacgtta acgtgccgat tgacggattc gacattgccg
     4501 cgtcggtcaa agaccacggg gacgcgcact tcctgcattt ggaccgacat gcccagagcg
     4561 ttggccaggg aataggccaa gacggtgagc gaaagacaat cgccactttt tcgacgccac
     4621 gtttccgcgg caattgtcga gtggcctccc gaataaggaa aatccttggt ttcccggccg
     4681 aacaggagcg agaccaaatg acgcgcacga tactgcgtgc tcgaattttg aatccttgga
     4741 tcctgcaatt tcgataagag ctcagggtca agctgaaaaa gatctcgctt gctgaccgag
     4801 accaaggccg cgtcgtagtc aaaagcttta tcctgccaca gaagatgcgc cggcaaaggc
     4861 gcacgcggcg ctgctgcaca tcctgcgaga acgatgaagc accccgtgat cgatagcgct
     4921 ttcagcagac ccccgatcat gttgccaacc tccgcgttcg gggtcc
//
LOCUS       DASPZD010000023         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_345555, whole genome shotgun sequence.
ACCESSION   DASPZD010000023 DASPZD010000000
VERSION     DASPZD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345555"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VEM33_00405"
     CDS             <1..1177
                     /locus_tag="VEM33_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYS50324.1"
                     /translation="AGLAGTLVLANAKLWAAPGDAKLLVVMLRGAYDGASLLVPYASD
                     FYYRSRPSIAVPRPGSGDPNAAIALDSDWALNAAVKDSLLALYKNRQAVLIPFSGSED
                     LSRSHFQAQDLMELGQGGGGALDYSSGYLNRLAETLWGSGSQSAVSFTNNLTAVFKGA
                     VPVPNISLRGNMKSNLPQRQSEMIASLYEGTKLSGMATAGMETKKLVSAELMNEMAES
                     SRGAGAARAFAVRARVIAKLMRDKPAYSIGFVDVGGWDTHVNQGSAQGALAANLSGLA
                     EGLSAFAQEMGGLWRNTVVVVMSEFGRTFRENGNRGTDHGHGNALWVLGGGISGGRIA
                     GRQVAVSEANLFQNRDYQVLNDYRAVLAYLFARMYGLGGAGLEHVLPGAKAESYGFI"
     gene            complement(1182..1625)
                     /locus_tag="VEM33_00410"
     CDS             complement(1182..1625)
                     /locus_tag="VEM33_00410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYS50325.1"
                     /translation="MSPEGAPAAPGSAGIKIPFTEHLGIQLVEMTKERAVVSLQKRPE
                     LLNSWGATHGGVIMTMLDLVMSWAVRGHYGVAGGVLTVDLSVGFMKGSFGDRVTAEGK
                     VLHGGKSTAFCEAEARDDKGALLAKAIGTFKLLADKEKKGGERAA"
     gene            complement(1615..>2201)
                     /locus_tag="VEM33_00415"
     CDS             complement(1615..>2201)
                     /locus_tag="VEM33_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYS50326.1"
                     /translation="LVCDISRPEAIEPMVSKVLERHGKVDVLVNNAGTTWGAPAEDHP
                     LDAWRKLVDLNLTGTFLVTQAVGKRSMIPNRYGRIINVASIAGLRGNPVDMLETLGYN
                     TTKGGLVNFTRALAAEWGEHGITVNAVAPGFFPSRMTKASLERLGEKIVARTPLRRLG
                     DGEDLKGLVVLLASDASRHITGQIIAVDGGASVA"
BASE COUNT          345 a          732 c          724 g          400 t
ORIGIN      
        1 cgcagggctc gccggcacgc tcgttctggc aaacgcgaag ttatgggccg cacccggcga
       61 cgcgaagctc ctcgtggtga tgctgcgcgg cgcctacgac ggcgccagcc tgctcgtgcc
      121 ttatgcgagc gatttctact accgctcgcg cccgagcatc gccgtgccgc gtccgggctc
      181 gggcgatcca aacgccgcga tcgccctcga ttcagattgg gcgctcaacg ccgcggtgaa
      241 ggattcgctc ctcgctctgt acaagaacag gcaggcggtg ctgattcctt tcagcggcag
      301 cgaggacctc tctcgcagcc acttccaggc ccaggacctg atggagctcg ggcagggcgg
      361 gggcggcgcg ctcgattact cgagcggata tctcaaccgc ctcgccgaaa cgctttgggg
      421 gagcggttcg cagagcgccg tatcattcac caacaacctc actgccgtct tcaagggcgc
      481 ggtcccggtt ccgaatatct cgctccgggg caacatgaag agcaatcttc ctcagcgtca
      541 gagcgaaatg atcgcctccc tctacgaggg gacgaagctt tctggcatgg cgacggcggg
      601 aatggaaacg aagaagttgg tctcggcgga gctgatgaac gagatggcgg agtcctcgcg
      661 cggggccggc gcggcgcgcg ccttcgccgt ccgcgcccgc gtgatcgcga agctgatgcg
      721 cgacaaaccc gcctactcga tcggtttcgt ggacgtgggc ggctgggaca cgcatgtcaa
      781 ccagggttcg gcgcagggcg cgctcgccgc gaacctctcg ggtctcgccg agggcctctc
      841 cgccttcgcg caggaaatgg gcggcctctg gcgcaacacc gtcgtcgtcg tcatgtcgga
      901 attcggccgc accttccgcg agaacggcaa ccgtggcacc gaccacgggc atggcaatgc
      961 gttgtgggtg ctcggcggcg gcatttcggg cggccgtatc gcggggcgcc aggtggcggt
     1021 gagcgaggca aacctcttcc agaaccgcga ttaccaggta ctgaacgact accgcgcggt
     1081 gctcgcctat cttttcgcgc ggatgtacgg gctcggcggc gcgggactcg agcacgtgct
     1141 gccgggcgcg aaggccgaga gctatggatt catttgagct gtcaggcggc ccgctctccg
     1201 ccttttttct ccttgtcggc gagcagcttg aaggtcccga tcgccttggc gaggagcgcg
     1261 cccttgtcgt cgcgcgcctc ggcttcgcag aaggcagtgg attttccgcc gtggaggact
     1321 ttgccttcgg ccgtcaccct gtcgccgaag ctccctttca tgaagccgac gctcaagtcg
     1381 accgtcagca cgccgccggc cacgccgtag tgcccgcgca ccgcccagct catgacgaga
     1441 tcgagcatgg tcatgatcac gccgccgtga gtggcgcccc agctgttcag gagctcgggg
     1501 cgcttctgca gggaaaccac cgcgcgttcc ttcgtcatct cgacgagctg gatgccgagg
     1561 tgctcggtaa acgggatctt gatgccggcg gagcccggcg ccgccggggc tccctcaggc
     1621 gacactcgcg cctccgtcga ctgcgatgat ctgcccggtg atgtgacgcg aggcgtccga
     1681 tgcgagcagc accacgagcc ccttcaggtc ctccccgtcg ccgaggcggc gcagcggcgt
     1741 gcgcgcgacg atcttctcgc cgagcctctc gagcgacgcc ttcgtcattc tcgagggaaa
     1801 gaatcccggc gcgactgcgt tcaccgtgat gccgtgctcg ccccattcgg cggccagcgc
     1861 gcgcgtgaaa ttcacgagcc cacccttggt cgtgttgtat ccgagcgtct ccagcatgtc
     1921 cacggggttg ccgcgcagcc ccgcgatcga cgcgacgttg atgattctgc catagcggtt
     1981 cgggatcatc gagcgttttc ctacggcctg ggtgacgagg aaggttcccg tcaggttgag
     2041 gtccacgagc ttgcgccacg catccagtgg gtgatcttcg gcgggcgcac cccaggtggt
     2101 ccccgcgttg ttgacgagga cgtccacttt cccgtggcgc tcgagcacct tcgaaaccat
     2161 cggttcgatc gcttccggcc gggaaatgtc gcagacgagc g
//
LOCUS       DASPZD010000024         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_707830, whole genome shotgun sequence.
ACCESSION   DASPZD010000024 DASPZD010000000
VERSION     DASPZD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707830"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VEM33_00420"
     CDS             <1..795
                     /locus_tag="VEM33_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HYS50327.1"
                     /translation="PVDERGRFFVAPYGSFGRMTRPVFIGEDRVAQYDVDEGRAGLDA
                     GAVLSTWGEARIGALWRHVDAAVNTGSPVLPSVKETTAGPRVHVFVDQLDHAFFARSG
                     YRAVASAYAAEESFGSDRNYKRLEGQFDAVTTWRAHTLNVTLAGGSDLGTDMPAYETF
                     TLGGPLRLSGYRIDEFAGRRMAFGRLMYYNRTVALPDVLGSGIYAGASLEAGQMKGRF
                     DGSSGAPTIASGSVFLGADTSLGPAYFGLGFGEGGRLSLYLLVGVP"
     gene            842..2011
                     /locus_tag="VEM33_00425"
     CDS             842..2011
                     /locus_tag="VEM33_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HYS50328.1"
                     /translation="MNCIPGIALGFAAALAAAGALAQPVPPPPPPPPGASAPPPPGTP
                     ASAAPTFKQEELEQLAAPIALYPDALVAQILMASTYPLEVVEAARWSKANPKLKDKAL
                     EDAMQQQRWDPSVKSLTAFPQVLAMMDQKLDWTQKLGDAFLAQQKELMAAVQRLRAKA
                     QAAGNLKSGKEQTVKVEQQDGSTVYIIEPANPQVVYVPTYNPVVVYGPWPYPAYPPYY
                     YYPPGYVAGPAFVFSVGIIIGSAMWGGCHWGGGNVYINHNTYNNFNRTNIGNGNWEHR
                     PEHRKGAQYRDTATQQRYGGGARPGVDSREAYRGRDAGAGAGQDLPRGGGDNFGQPDR
                     AGDRAAGAGGFGGGREAGAFDGVGRGGETRDFSNRGATSRASAGASRGGGGGRRR"
     gene            2031..2954
                     /locus_tag="VEM33_00430"
     CDS             2031..2954
                     /locus_tag="VEM33_00430"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HYS50329.1"
                     /translation="MRRLEFSVLALAFALAAAANPATAATGAKQKSFATPEEAVKQLA
                     AAARSGDSRALVEILGPDAKAIVASGDPVTDKKSRERFARAYDEANKLERSGDARAVL
                     SVGKDEWTFPIPIVKDAAGWRFDTKAGKEEILNRRVGRNERHAVQAALAYVDAQREYY
                     LRNPQGDKMLQYAQKLRSANGKRDGLYWPARAPEQPSPLGPVYAAARAEGYAKDSSGR
                     PYPYQGYFYRILKGQGPEARGGAYDYVVQGRMIGGFALVAYPAEYGNSGVMTFLVNHD
                     GTVYEKDLGPETGRIAQKISKFNPDKAWKAL"
BASE COUNT          553 a          982 c         1027 g          428 t
ORIGIN      
        1 ccggtggacg aacgcggccg cttcttcgtc gcgccctacg gaagcttcgg ccggatgacg
       61 cgccccgtct tcatcgggga ggacagggtc gcgcaatacg acgtggacga ggggcgcgcc
      121 gggctcgatg ccggggcggt cctcagcacc tggggcgagg cgcgcatcgg cgcgctgtgg
      181 cggcacgtgg acgcggcggt gaacacgggc tcgcccgtgc tgccgagcgt taaggaaacg
      241 acggcgggtc ccagggtgca cgtcttcgtg gaccagctcg atcacgcctt cttcgcccgc
      301 agcggctacc gagccgtcgc ttccgcgtac gccgccgaag aaagtttcgg gtcggaccgc
      361 aattacaagc gattggaggg gcagttcgac gcggtcacga cctggagggc ccataccctg
      421 aacgtcacac ttgccggagg cagcgacctg ggcacggaca tgcctgccta cgagaccttc
      481 acgctgggcg gcccgctgcg gctgtcggga taccgcatcg acgaatttgc cgggcgccgc
      541 atggcgttcg gccggctcat gtactacaac cggacagttg cgctgcccga tgtgctcgga
      601 tcggggatct acgcgggcgc atcgctcgaa gcaggccaga tgaaggggcg gttcgacggc
      661 agttccgggg cgccgacgat cgcgtcgggc tccgtgttcc tgggcgcgga cacctccctc
      721 gggcctgcct acttcggatt gggcttcggc gaaggcgggc gcttgtcctt gtatctgctc
      781 gtcggcgtgc cgtaacgcct gcgcgcgacg aagagattaa gattcaggaa agcgaggtga
      841 tatgaattgc attcccggaa tcgcgctggg gttcgccgcc gcgcttgcag cagcgggcgc
      901 cctcgcgcag ccggtaccgc cgcctccgcc cccgccccct ggggcatcgg caccacctcc
      961 tcccggcacg ccggcatccg ccgcaccgac gttcaaacag gaagagctcg agcagctcgc
     1021 ggcgccgatc gcgctctatc cggacgcgct cgtcgcgcag atcctgatgg cctcgaccta
     1081 tccgctggaa gtggtcgagg cggcgcgctg gtcgaaggcc aacccgaagc tgaaggacaa
     1141 ggcgctcgaa gacgcgatgc agcagcaacg ctgggacccg agcgtgaagt ccctcaccgc
     1201 cttcccgcag gtgctggcga tgatggacca aaagctcgac tggacgcaga agctcggcga
     1261 cgccttcctc gctcagcaga aggagctgat ggccgcggtc cagcggctgc gcgccaaggc
     1321 gcaggcggcg gggaatctca agtccggcaa ggagcagacg gtcaaggtcg agcagcagga
     1381 cgggtcgacc gtctacatca tcgagcccgc gaatccgcag gtggtctacg tgccgaccta
     1441 taacccggtg gtcgtctacg gtccctggcc ctacccggcg tatccgccct actactacta
     1501 tccgccgggc tacgtcgcag gtccggcatt cgtgttctcg gtgggcatca tcatcggctc
     1561 ggccatgtgg ggcgggtgcc attggggcgg cggtaacgtc tacatcaatc acaacaccta
     1621 caacaacttc aaccgtacca acatcggcaa cggcaactgg gagcacagac cggagcaccg
     1681 caagggcgcg caataccgcg acacggcgac ccagcagcga tacggcggcg gcgcaaggcc
     1741 cggggtcgat tcgcgcgaag cttaccgcgg ccgcgacgca ggcgcaggcg cagggcagga
     1801 tctcccgcgc ggcggcggcg acaatttcgg acagccggac agagccggag atcgcgcggc
     1861 cggcgcgggc ggtttcggcg gcggcagaga agcgggcgct ttcgacggag tcggacgtgg
     1921 cggtgaaacc cgcgacttca gcaatcgcgg cgcgacgagc cgcgcatcgg cgggagcgtc
     1981 gcgcggcggc ggtggcggca ggcgcagatg aagcgcgagg agacacgcac atgagacgcc
     2041 tcgaattctc cgtccttgcc ctggccttcg cccttgcagc cgcggcgaat cctgcgacgg
     2101 ccgcgacggg cgcgaaacag aaaagtttcg ccacacccga ggaggccgtg aagcagctcg
     2161 cggcggctgc gaggtcgggc gattccaggg cgctggtcga gatcctcgga cccgacgcca
     2221 aggcgatcgt cgcttcgggc gatccggtca ccgacaagaa aagccgcgag cgtttcgccc
     2281 gcgcttacga cgaggcgaac aagctcgaaa gatcgggcga cgcccgggcg gttctcagcg
     2341 tcggcaagga cgagtggacc tttcccattc ccatcgtcaa ggacgccgcg ggctggcgct
     2401 tcgacacgaa agcaggcaag gaagagatcc tcaatcggcg cgtcggacgc aacgagcgcc
     2461 atgccgtcca ggcggcgctc gcctacgtgg acgcgcagcg cgagtactac ctgcgcaatc
     2521 cgcaaggcga caagatgctg cagtacgcgc aaaaactccg cagcgcgaac ggcaagcgcg
     2581 acggcctgta ttggcccgcg cgcgccccgg agcagcccag cccgctcggg ccagtctacg
     2641 cggcggcgag ggcggaaggc tacgccaagg attcgagcgg caggccctac ccctaccagg
     2701 gctacttcta ccgcatcctc aagggacagg gcccggaggc gcgcggcggc gcctacgatt
     2761 acgtggtgca agggaggatg atcggcggct tcgcgctcgt cgcgtatccg gccgagtacg
     2821 gcaactccgg cgtcatgact ttcctcgtca accatgacgg caccgtatac gagaaagacc
     2881 tggggccgga aaccgggcgc atcgcacaaa agatttcgaa gttcaatccg gacaaggcct
     2941 ggaaggcatt gtgaacagcg tgctggcaat agtgagaagg ctcccacaat
//
LOCUS       DASPZD010000025         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1434148, whole genome shotgun sequence.
ACCESSION   DASPZD010000025 DASPZD010000000
VERSION     DASPZD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434148"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VEM33_00435"
     CDS             <1..571
                     /locus_tag="VEM33_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HYS50330.1"
                     /translation="KFLLSVSRLIDALNERIGHIVYWLVLIMVLVSAGNATSRYVFSI
                     ASNAWLELQWYLFAAVFLLCSGYTLRHNEHIRIDVIAGNLSRRAQMWIDVFGTLFFLL
                     PMAAFITWLSWPIFMNAWNSNEISGSAGGLIRWPARLLVPVGFFLLSLQGLSELIKRI
                     AFLKGLIPDPLEKHEVPMELGIARDEAAK"
     gene            568..2121
                     /locus_tag="VEM33_00440"
     CDS             568..2121
                     /locus_tag="VEM33_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYS50331.1"
                     /translation="MTQFLIANVAPLMFAALVVFLLIGYPVSFALAAIGLSFGFLGIE
                     LGLLTPALFGALPERIFGIMSNDTLLAVPFFTFMGLILERSGMAEDLLNTIGQLFGPI
                     RGGLAYAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRLASGVIAASGTLA
                     QIIPPSLVLIVLADQLGRSVGDMYAGAIFPGLVLTALYAIYVFGVTLIYPKAAPALPP
                     EARTLRGAKLLARVFLVLIPPLALIFLVLGTIFLGVATPTEGGAMGATGALVLAMINR
                     RLSWSLLRQAMNSTAKLTSFVVFILVGSTVFSLTFRGVNGDLWVEHLLTGLPGGAIGF
                     LIVVNILVFLLAFFLDFFELAFIIVPLLGPVADKLGIDLIWFGVLLGVNMQTSFMHPP
                     FGFALFYLRSVAPGKEYLDKLTGKLTPPVTTGQIYWGAVPFVVIQVVMVGIVIGFPGL
                     VTGNLVKSSGDPSKVQSFVPAESEDTRDQTPPDFGTPGDVKSDAERKSGESAREKRKA
                     DDLENLFKK"
     gene            complement(2195..3310)
                     /gene="dctP"
                     /locus_tag="VEM33_00445"
     CDS             complement(2195..3310)
                     /gene="dctP"
                     /locus_tag="VEM33_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HYS50332.1"
                     /translation="MKRREFIRSAGLGAAAGAAMLTVAEARAQQTSGLPNLQWRLAAS
                     WPKSLDTLFGGAELVAKRVGEITDGKFQIRAFAAGEIVPALQVLDAVQAGTVELGHTA
                     TYYYFGKDPTFALGTAVCFGMNTRQNNAWWYFGGGSEAMAPLFKEYGCIALLSGNTGC
                     QMGGWFRKEIKSVADLKGMKMRIGGMAGLVLAKLGVVPQLIGAPDIYPALEKGTIDAA
                     EWVGPYDDEKLGFNKVAKYYYYPGFWEGGPMLMTLVNEKKWNELPKPYQAALSAACGE
                     VNSWMPAKYDEQNPQALRRLIASGTNLRPFPRSVMEAAEKASYELYDELKVKSKHWAT
                     VYPQWKKFRDEQFLWFRVAESTYDNYSFASKLGAGKS"
     gene            complement(3368..4648)
                     /gene="pmbA"
                     /locus_tag="VEM33_00450"
     CDS             complement(3368..4648)
                     /gene="pmbA"
                     /locus_tag="VEM33_00450"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HYS50333.1"
                     /translation="MAYAVEKGASACETESSDGYGQTVTVRKGEVETIEYNRDKSLAV
                     TVYVGKRKGHASTADFAPRAMRDTVEAALTIARFTAEDESAGLPDPEELARGPQDLDL
                     FHPWDLPVERAIGIAKACEAAAFRLDKRVVNSEGASVSCQQWHFALANSHGFLEGFPT
                     SRHTISCAVIAAEDGRMQRDDWYVSQRSPQDLAQPEAIGDYAGRRALARLRSRKIGTV
                     QAPVLFEAPVANDLLATFVSAVSGGNLYRKASFLLDSLGKEVFAPIVTLVERPHLPRA
                     PASSWYDDEGVATRDRDVVKDGVLRGYFLGSYSARKLGMKTTGSAGGNHNLILEGGEL
                     DFPGLIRTMGRGLLVTELIGFGVNLVTGDYSRGAAGYWVEGGEIRYPVEEITIAGNLR
                     DMFRSVAAIGADKLTRGSRTTGSILIDGMTIAGN"
BASE COUNT          741 a         1543 c         1434 g          938 t
ORIGIN      
        1 gaaattcctg ctctccgttt cccgcctcat cgatgcactg aacgagcgca tcgggcacat
       61 cgtgtactgg ctggtcctca tcatggtgct ggtgagcgcc ggcaacgcca cgtcccgcta
      121 cgttttctcg atcgcctcca acgcgtggct cgagctgcag tggtatctgt tcgcagcggt
      181 cttcctgctt tgctccggat acacgctgcg gcacaacgag cacatccgga tcgacgtgat
      241 cgccggcaat ctgtcgcggc gcgcgcagat gtggatcgac gttttcggca cgctcttctt
      301 cctgctgccg atggcagcct ttatcacgtg gctctcctgg ccgatattca tgaacgcctg
      361 gaacagcaac gagatctccg gcagcgcggg cggcctcata cgctggccgg cgcggttact
      421 ggttccggtc ggcttcttcc tgctgtcgtt acagggcctc tccgagctga tcaagcgcat
      481 cgcattcctc aagggcctga ttccagaccc gctggaaaag cacgaggtcc cgatggaact
      541 gggcatcgcg cgcgacgaag cagccaaatg acgcagtttc tcatcgccaa cgtggcgccg
      601 ctgatgttcg cggcgctcgt ggtgttcctg ttgatcggct atccggtttc gttcgcgctg
      661 gcggcgatcg gcctgtcgtt cggctttctc ggcatcgagc tgggcctgct cacgccagcg
      721 ctgttcgggg cgctgcccga gcggattttc ggcatcatgt cgaacgacac gctgctcgca
      781 gtgccgttct tcaccttcat ggggctgatc ctcgagcgca gcggcatggc cgaggatctg
      841 ctcaacacca tcgggcagct cttcggtccg atccgagggg ggctcgctta cgccgtgatc
      901 ttcgtaggcg cgctgcttgc cgcgaccacc ggcgtggtgg cggcctcggt gatctcgatg
      961 gggctcattt cgctgccgat catgctgcgc tacggctacg atcgacgcct ggcgagcggc
     1021 gtcatcgccg cctccgggac gctggcgcag atcattccgc cgtcgctggt cctcatcgtg
     1081 ctcgcggacc agctcgggcg atcggtcggc gacatgtacg ccggggcgat ctttcccggg
     1141 ctcgttctca ccgcgctgta cgcgatctat gtcttcggcg ttacgctgat ctatccaaag
     1201 gccgcgccag cactgccgcc ggaagcgcgc acgctgcgcg gcgctaaatt gctcgcgcgc
     1261 gtattcctgg tgctgatccc gccgctcgcc ctgatcttcc tcgttctcgg caccattttc
     1321 ctcggcgtag cgacgccgac ggaaggcggc gccatgggcg ccaccggcgc cctggtgctg
     1381 gcaatgatca accggcgcct gagctggtcg ctgcttcggc aagcgatgaa ttcgacggcg
     1441 aagctcacct cgttcgtggt gttcatcctg gtcggctcga cggtgttcag cctgaccttc
     1501 cgcggcgtca acggcgatct ctgggtcgag cacctgctca cgggactccc gggcggggca
     1561 atcggcttcc tgatcgtggt gaacatcctg gttttcctgc tcgctttttt cctcgatttc
     1621 ttcgagctgg cgttcatcat cgtgccgctg ctcggaccgg tggcggacaa gctcgggatc
     1681 gatttgatct ggtttggggt gctgctcggc gtgaacatgc agacttcctt tatgcatccc
     1741 ccgttcggtt tcgcgctgtt ctacctgcgc agcgtggcac ccggcaagga gtacctcgac
     1801 aagctgaccg gcaagctgac gcctccggtc actaccgggc agatctattg gggagcggtg
     1861 cccttcgtgg tgattcaggt cgtcatggtc ggtatcgtca tcggttttcc cgggctggtc
     1921 accgggaacc tggtgaagag cagcggcgat ccgtccaagg tccagagctt cgttcccgcg
     1981 gagagcgagg atactcgaga ccagacgccg cccgacttcg gcacacccgg cgacgtgaag
     2041 tccgatgcgg agcggaaatc aggcgaaagc gcaagggaga agcgcaaagc cgacgacctc
     2101 gagaatctct tcaagaaata accggcagcg cgcccccaaa aaacaaaccc cgccgaagcg
     2161 gggttcgcat tgcagcggta cgcgctcgcc ggagctacga cttcccggca ccgagcttgg
     2221 aggcaaacga atagttgtcg taggtcgatt cggcgacgcg gaaccacagg aactgctcat
     2281 cgcggaattt cttccactgc ggataaacgg tcgcccagtg cttgctcttc accttgagct
     2341 cgtcgtagag ctcgtaggac gctttctcgg ccgcttccat caccgatctg gggaacggcc
     2401 gcaggttggt gccgctcgcg atcagccggc gcagcgcctg cgggttctgc tcgtcgtatt
     2461 tggccggcat ccacgaattc acttcgccgc aggcggcgga cagtgcggcc tggtagggct
     2521 tgggcagctc gttccatttc ttttcgttga cgagcgtcat cagcatcggg ccgccttccc
     2581 agaagccggg atagtagtag tacttggcga ccttgttgaa gccgagcttc tcgtcgtcgt
     2641 agggccccac ccattcggcc gcatcgatcg tgcccttttc gagcgcgggg tagatgtccg
     2701 gagccccgat caattgcggt acgacgccca gcttcgcgag caccaggccc gccatgccgc
     2761 cgatcctcat cttcatgccc ttcaggtcgg ccaccgattt gatctccttg cggaaccagc
     2821 cgcccatctg gcagcccgtg tttccggaga ggagcgcgat gcagccgtat tccttgaaca
     2881 gcggcgccat cgcctcggaa cctccgccga aataccacca agcgttgttc tggcgcgtgt
     2941 tcatgccgaa gcacaccgcg gtgcccagag cgaacgtcgg atccttgccg aaatagtaat
     3001 aagtcgccgt gtggcccagc tcgaccgtac cggcttgcac ggcgtcgagc acctgaagcg
     3061 ccggcacgat ctcgcccgcc gcaaaggcgc ggatctggaa cttgccgtct gtaatttcgc
     3121 cgacgcgttt cgcaacgagc tcggcgccgc cgaacagcgt atccaggctc tttggccagc
     3181 tggctgccag gcgccactgg agattcggca ggccggaagt ctgctgtgca cgggcctctg
     3241 ccacggtgag cattgccgcg cccgcggccg cgcccagccc agcgctcctg atgaattcgc
     3301 gtcttttcat ggggtccctc ctcaaggtgg aaatccgggc cgattatacg cccggtcgaa
     3361 aagccctcta gtttcctgcg atggtcattc cgtctatgag aatcgagccg gtggtgcgcg
     3421 agccgcgcgt cagcttgtcg gctccgatcg ccgccaccga gcggaacatg tcgcgcaggt
     3481 tgcccgcgat ggtgatttcc tccacgggat accggatctc gcctccctcc acccagtagc
     3541 ccgccgcgcc gcgcgagtag tcgccggtaa cgagattcac tccgaacccg atcagctcgg
     3601 tgacgagcag tccgcgcccc atcgtcctga taaggccggg aaaatcgagc tcgccgccct
     3661 ccaggatcag attgtgattg ccgcccgcgc tgccggtggt cttcatcccg agcttgcgcg
     3721 cggagtagct cccgagaaag taaccccgca acaccccgtc cttcaccacg tcgcgatcgc
     3781 gcgtcgcgac gccttcgtcg tcgtaccagg agctcgccgg cgcgcgcggc aagtgcggcc
     3841 gctcgaccaa agtgacgatc ggcgcgaaga cttccttgcc gaggctgtcg agcagaaacg
     3901 aagccttgcg gtaaaggttt cccccgctca ccgccgagac gaaggtcgcg agcaagtcgt
     3961 tcgccacggg cgcctcgaac agcaccggcg cctgcaccgt tccgatcttg cggcttctga
     4021 gccgtgcgag ggcacgcctc ccggcgtagt cgccaatggc ttcgggctgg gccaggtctt
     4081 gcggggaacg ctgcgatacg taccagtcgt cgcgctgcat ccggccgtcc tctgcggcga
     4141 tcaccgcgca ggagatcgtg tgccgcgacg tgggaaaacc ttccaggaaa ccgtggctgt
     4201 tggcgagggc gaaatgccat tgctggcagg agacgctggc gccctcggag ttcaccacgc
     4261 gcttatccag gcggaatgcg gcggcctcgc aggccttcgc gatcccgatt gcccgctcga
     4321 cgggcagatc ccagggatgg aagaggtcga ggtcttgcgg cccgcgcgcc agctcctccg
     4381 gatccgggag ccccgcgctc tcgtcctcgg cagtgaagcg cgcgatcgtc aaggccgcct
     4441 ccaccgtatc gcgcatcgcg cgcggggcaa aatctgcggt gctcgcgtgg cctttgcgct
     4501 tgccgacgta aacggtgacc gcgagactct tgtcgcggtt gtattcgatg gtctcgacct
     4561 cgcccttgcg caccgtcacc gtctgaccgt agccgtcgga gctttcggtt tcacaggcgc
     4621 tcgcgccttt ttcaacggcg tacgccaagg catccg
//
LOCUS       DASPZD010000026         6199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_104598, whole genome shotgun sequence.
ACCESSION   DASPZD010000026 DASPZD010000000
VERSION     DASPZD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104598"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2755
                     /locus_tag="VEM33_00455"
     CDS             <1..2755
                     /locus_tag="VEM33_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50334.1"
                     /translation="AASQQLISPAQGVAPLTLPGGVDAERERAGPLEAIKLYDELLAK
                     YPQYAFRDQVLYQKARAYDELGQTEAALKVMEQLGREYPHSRFGDEVQFRRAEHFFIA
                     RKYRDAESAYTAIADSGAGSEYYELALYKLGWTLYKQQLYQEALQRYFALLDYKVKSG
                     YDFDAKHTEAEQRRVEDTFQVVSLSFSNIGGPDVIGSYFATNGHKAYEDRVYRYLAEF
                     YLVKLRYQDAATIYKSFVALYPYHQASPHYSMRVVEIYEKGSFPQLVLAAKKDFAGTY
                     GLQGEYWHHVDVNKSPEVLSYLKSNLKDLANYYHAQYQDPKQHEQRPGNYDEATRWYR
                     EFLTSFHTDSQAPQVDYQLADLMLENHDYPAAAREYEITAYDYPPHAKSAAAGYAAIY
                     AHREYLKVASVDLKDAARRDTINSSIKFAGAFPQHEQAPVVLAAAAQDAYELKDLMLA
                     RDTARRLIEKFPNAAAGVRRDAWLVVAHASFGLAEYGDAEQAYGRVLEATPGGDASHA
                     ALVENLAASIYKQGEQASQAGDYRTAANHFVRVKQVAPTSKICAAAEYDAGAALLHLQ
                     DWTAAAQVLEEFRRSFPEHELQKDATKQIAYAYQQSGQLAQSAGEYERVARESPNPQL
                     RAEALLQAGDLYGQAKDAERALQAYSRYVEEFRKPIDAAVETRSKIAEIYKTKGDQGQ
                     YRHQLEEIVRVDGAAGSERTDRTRNVAARAALALSEPMYAQFASLKLAQPFERSLQEK
                     QRGMDAATKVFGALVDYQVGEVTAAATFYMAEIYANFSQALRESERPADLNADALKDY
                     ERQLDEAAQPLGQKAVAVHEKNLELMRRGLYSAWTQKSLDRLAALKPDTYARTEISSG
                     FLGSLERYVYRPPPRAAQTAARAEVQGAPQPENAALMARPPGRADSAAGQVAAGAGNA
                     ITR"
     gene            2757..3389
                     /locus_tag="VEM33_00460"
     CDS             2757..3389
                     /locus_tag="VEM33_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50335.1"
                     /translation="MARTTSLRHAAAGLVLAVALVACATTVPATKAHGTVAARQPEAG
                     GFQLNEKVRVPADARTDYDNAMRLVDAAQYDQGIALLQRVTQKAPAAAAAYVDLGIAY
                     GRSGDIDHAVASLKRAVELNPRQLVAYNELGILYRRKGEFSAARESYEKALGIFPGFH
                     YARLNLAILCDVYLGDLACARENYAAYQAAVPDDGQVVAWIADLNTRAAR"
     gene            3399..3803
                     /locus_tag="VEM33_00465"
     CDS             3399..3803
                     /locus_tag="VEM33_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50336.1"
                     /translation="MLPKLILCLPLLLGAAAAQADGPATAAAAPSEAATAAPADVAKE
                     PPKKRTADPKTDPASATSGPTPPGGDVKMSGISILGNEDSPKSLVLVPWKSSQLGDMP
                     SVSRLLDSSKQPVDKEVFLRELSYYEFRAGTK"
     gene            3875..4561
                     /locus_tag="VEM33_00470"
     CDS             3875..4561
                     /locus_tag="VEM33_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYS50337.1"
                     /translation="MDFFYSIVRSFTAGGVFMLPILFTGALAAAITIERYVTLAKMGA
                     GNRRTWSGVEPAIASGDFDKARELTSKDDSPIARVLSMGLALQGAVRRRDDIEKAMQE
                     SMMEIVPRLEKRTHYLATLANVATLLGLLGTVSGLIHAFAAVATVNPAEKANLLSASI
                     SEAMNCTAFGLITAVPILVLHAFLQSKTMGLSDGLETAAIRFLNAVAAREAARAHSRM
                     ELVRQAASAA"
     gene            4572..5111
                     /locus_tag="VEM33_00475"
     CDS             4572..5111
                     /locus_tag="VEM33_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYS50338.1"
                     /translation="MRSLLMRREPKPAELLLVPMIDIFTVLVTFLLMTAVFSRIAIMQ
                     IDLPSSAPAKPAEPKFRLEVIVRQDGFELSDGNQLIATIPKVNGAYDMNALTERALAV
                     KREHPTSEDASVLSEPKVPYDELIQAMDAIRSAELPAAEALVAGQRVTGAQAGAADAR
                     TPQTARVALFTNIALGDAP"
     gene            5108..5623
                     /locus_tag="VEM33_00480"
     CDS             5108..5623
                     /locus_tag="VEM33_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYS50339.1"
                     /translation="MKVLNLGLMPRKRRERVDAQLNLIPLIDILSVMVAFLLVYSTDV
                     EVLQNSQGVSIPQSQTDTRPKQSVVVMVTKEALLVQGETVASLAEVSDPRTQLIEPLR
                     RVLQRPIAGLDAAARDAALTSQEVTVMADKDLPYEVLKKVLATCTAAAYGKISLAVLQ
                     KEKPVSGVKGS"
     gene            5645..>6199
                     /locus_tag="VEM33_00485"
     CDS             5645..>6199
                     /locus_tag="VEM33_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50340.1"
                     /translation="MTVISPQGNGMNAEIAQEQQTLEDQRTPEDQQAPNKEALQDQGT
                     LLAELGTSQESVDALVGELRSLDAQLEALAAERNQHRLLHQVCDALGELGKLGGAQLF
                     WGTAQAAKSGEEQLGRVHERVAAFESRVGELEARRQSTREQIDQEQYRVGLIEDGLFD
                     AQEQEERLRQEWIVEREISGLHSRA"
BASE COUNT         1178 a         1932 c         2161 g          928 t
ORIGIN      
        1 ggctgcgtcg cagcagctaa tctcgccggc gcaaggcgtc gcgccgctga cgctgccggg
       61 cggcgtggat gcggagcgcg agcgcgccgg tcccctggag gcgatcaagc tgtacgacga
      121 gctcctggcc aagtaccccc agtacgcctt ccgcgatcaa gtgctctatc agaaggcgcg
      181 cgcctatgac gagctcgggc agaccgaggc ggcgctgaaa gtcatggagc agctgggccg
      241 cgagtatccg cactcgcgct tcggagacga ggtgcagttc cgccgcgccg agcatttctt
      301 cattgcgagg aaatatcggg acgcggagag cgcgtatacg gcgatcgcgg acagcggggc
      361 cggctccgag tactacgagc tcgcgctcta caagctggga tggacgttgt acaagcagca
      421 actctaccag gaggctctgc aacggtactt cgcgctcctc gactacaagg tcaagagcgg
      481 ctatgacttc gatgcgaagc acaccgaggc cgagcagcgg cgcgtcgagg atacctttca
      541 ggtcgtcagc ctgagcttct cgaatatcgg cgggcccgac gtgatcggct cgtacttcgc
      601 caccaacgga cacaaggcct atgaggaccg ggtgtaccgc tacctcgcgg agttctacct
      661 ggtcaagctc cgttatcagg acgccgcgac gatctacaaa tcgtttgtgg cgttgtatcc
      721 gtaccatcag gcatcgcctc actacagcat gcgggtggtc gaaatctacg agaagggcag
      781 cttcccgcag ctcgtgctcg ccgcgaagaa ggacttcgcg ggcacctacg gcctacaggg
      841 ggagtactgg catcacgtcg acgtgaacaa gtcgccggaa gtgctgagtt acctgaagag
      901 caacctgaag gatctggcga actactatca cgcccagtat caggacccga agcagcatga
      961 gcagcggccg ggcaactacg acgaggcgac gcgctggtac cgggagttcc tcacctcgtt
     1021 ccacaccgac agccaagcgc cgcaagtgga ctaccagctc gcggatctca tgctcgagaa
     1081 tcatgactat ccggcggcgg cgcgcgagta cgagatcacg gcttatgatt acccgccgca
     1141 cgcgaagtcg gcggccgccg gctacgcggc gatctacgcg caccgcgagt atctgaaggt
     1201 cgccagcgtc gacctgaagg acgcggcccg ccgcgacacg atcaacagtt cgatcaagtt
     1261 tgcgggcgca ttcccgcagc acgagcaggc accggtcgtg ctggccgcgg cggcacaaga
     1321 cgcatacgag ttgaaggact tgatgctggc gcgcgacacc gcccggcgcc tcatcgaaaa
     1381 gttcccgaat gcggccgcgg gtgtgcgccg cgacgcgtgg ctcgtggtgg cgcacgcatc
     1441 cttcggtctc gccgagtatg gggacgccga gcaggcctat ggccgcgtgc tggaggcgac
     1501 accgggggga gatgcatcgc acgccgccct ggtggagaac ctcgcggcct cgatctacaa
     1561 gcagggcgag caggcgagcc aggcgggcga ttaccgcacc gcggcgaatc acttcgtgcg
     1621 cgtcaagcag gtggctccga cgtcgaagat ctgcgcggcg gcggagtacg acgccggcgc
     1681 ggccctgctg cacttgcagg actggacggc tgcggcgcag gttctggagg agttccggcg
     1741 cagcttcccc gagcacgagc tgcagaagga cgcgaccaag cagatcgcct acgcctatca
     1801 gcagtccggc cagctggcgc agtcggccgg cgaatatgag cgcgtcgcgc gagagtcgcc
     1861 gaatccgcag ctgcgggccg aagcgctgct gcaggcgggc gatctctacg ggcaggccaa
     1921 ggacgcggag cgcgccctgc aggcgtactc ccgctatgtc gaggagttcc gcaagccgat
     1981 cgacgcggcg gtggagacac gctcgaagat cgcggagatc tacaagacca agggagatca
     2041 gggtcagtac cggcaccagc tcgaggagat cgtgcgggtc gatggagcgg cggggagcga
     2101 gcgcacggat cgcacgcgca atgtcgcggc gcgcgccgca ctggccctct cggagccgat
     2161 gtacgcgcag ttcgcgtcgt tgaagctcgc acagccgttc gagcgaagct tgcaggagaa
     2221 acaacggggc atggacgcgg cgaccaaggt gttcggtgcg ctggtcgact accaggtcgg
     2281 ggaggtcacg gctgcggcca ccttctacat ggccgagatc tacgcgaatt tcagtcaggc
     2341 cctgcgtgag tcggagcgcc cggcggatct caacgcggac gctctcaagg actacgagcg
     2401 ccagctcgac gaagccgcac agcccctcgg gcaaaaggct gtggcggtcc acgagaagaa
     2461 cctcgagctc atgaggcggg gactgtacag cgcatggacc cagaagagcc tggaccgtct
     2521 cgcggcgctc aagcctgaca cctacgcacg gaccgagatc agcagcggtt tcctgggttc
     2581 gctcgagcgg tacgtctatc ggccgccgcc gcgcgcggcg cagacggccg cgcgcgcgga
     2641 agtgcagggc gcgccgcagc cggaaaacgc ggcgttgatg gcgcgacccc cggggcgggc
     2701 ggatagcgcc gccggacaag tggcggcagg agcaggcaat gcgatcacgc gctgaaatgg
     2761 cacggaccac gagcctcagg cacgcggcgg ccggcctcgt gctggcggtc gcgctcgtcg
     2821 cctgcgcgac gaccgtgcct gcgaccaagg cgcacgggac agtggcggcg cggcagccgg
     2881 aggcgggtgg gtttcagctc aatgagaaag tgcgcgtccc cgcggacgct cgtaccgact
     2941 acgacaacgc gatgcgcctg gtggacgccg cgcaatacga tcaggggatc gcgttgctgc
     3001 agagggttac ccagaaagca cccgctgccg cggcggcgta cgtcgatctc ggcatcgcct
     3061 acggcaggtc cggagacatc gaccacgcgg tggcaagtct caagcgcgca gtcgagctca
     3121 accctcgcca gctcgtggcg tacaacgagc tcggcatcct ctaccgccgc aagggcgagt
     3181 tcagcgccgc ccgcgagagc tacgagaagg cactgggaat attcccggga tttcactacg
     3241 cgaggctgaa tctcgcgatt ctctgcgatg tgtacctggg tgatctcgcg tgcgcgcggg
     3301 agaactacgc cgcctaccag gcggcggtgc cggacgacgg ccaggtcgtg gcgtggatcg
     3361 ccgatctgaa cacgagagcc gcgcgctagg agggactcat gttgcccaaa ctcattcttt
     3421 gtctgccact gctccttgga gcagccgcgg cccaggcgga cgggccggcg accgcagcgg
     3481 cggcgccgag cgaggccgcg acggcggcgc cggctgatgt ggccaaggag ccgcccaaga
     3541 agcgcacggc ggatcctaag acggatccgg cgtcggcgac cagcgggccg acgccgcccg
     3601 gcggcgatgt gaaaatgtcg ggtatatcga tcctcggaaa cgaggacagc ccgaagtcgc
     3661 tcgtgctggt tccctggaag agctcccagc tcggagacat gccgagtgtc tcgaggctgc
     3721 tcgactcgtc gaagcagccg gtggacaagg aggtgttcct gcgcgagctc tcctattacg
     3781 agttcagagc gggcacgaag tgagcgggcc cgccggaatt gagaccggat cctgaagtgg
     3841 tttgacagat cgtatcgttt caagaggtat gcatatggac ttcttctatt caatcgtcag
     3901 atctttcacc gcaggcgggg tgttcatgct cccgatcctg ttcacggggg cgctcgccgc
     3961 cgcgatcacg atcgagcgct acgtcacgct cgcgaagatg ggcgccggca atcgacgcac
     4021 gtggagcggt gtcgagccgg cgatcgcgag cggggacttc gacaaggcgc gcgagctcac
     4081 gagcaaggac gattcaccga tcgcgcgagt gctgagcatg gggctcgccc tgcagggggc
     4141 ggtgcggcgg cgcgatgaca tcgagaaagc catgcaggaa agcatgatgg agatcgtgcc
     4201 gcggctcgag aagcgcaccc actacctcgc caccctggcc aatgtcgcga cgctgctcgg
     4261 cctgctgggc acggtgagcg gcctgatcca cgccttcgcc gcagtggcga cggtgaaccc
     4321 ggctgagaag gcgaacctgc tgtcggcgag catctcggag gccatgaact gcacggcctt
     4381 cgggctcatt accgcagtgc cgatcctggt gctgcacgcc ttcctgcagt cgaagaccat
     4441 ggggttgagc gatggtctgg agacggcggc catccgcttc ctgaacgcgg tcgccgcgcg
     4501 cgaggcggcc cgggcccata gccgcatgga gctcgtacgg caagcggcgt cggcggcctg
     4561 aggatcacgc catgcgcagc ctcctgatgc gtcgcgagcc caagccggcc gagctgctgc
     4621 tcgtgccgat gatcgacatc ttcaccgtcc tggtcacctt tctgctgatg acggcggtgt
     4681 tctcgcgcat cgcgatcatg cagatcgacc tgccgagctc cgcgcccgcg aagcctgcgg
     4741 agccgaagtt ccgactcgag gtcatcgtcc gccaggacgg cttcgagctg agcgacggca
     4801 atcaactgat cgctacgatc ccgaaggtga acggcgcata cgacatgaac gcgctgaccg
     4861 agcgcgcgct ggcggtgaag cgcgagcatc cgacctcgga ggacgcgtcg gtactctcgg
     4921 agccgaaggt tccctacgac gagctgatcc aggcgatgga tgcgatccgc agcgcagagc
     4981 tgccggctgc agaagcgctg gtcgccggtc agcgcgtaac gggagcgcaa gccggcgcgg
     5041 cggacgcgcg cactccgcag accgcgagag tcgcgttgtt cacgaacatt gcgctcggag
     5101 atgcgccatg aaagtgctga atctcggcct gatgccgcgc aagcgacgcg agcgcgtcga
     5161 tgcgcagctg aatctgatac cgctcatcga cattctctcg gtgatggtgg cgttcctgct
     5221 ggtgtactcg acggacgtgg aggtactgca gaactcccag ggagtgagca tcccgcagtc
     5281 gcaaaccgat acgcgcccga agcagtcggt cgtcgtgatg gtcacaaagg aggcgctcct
     5341 ggtgcagggt gagacggtcg cgagcctcgc ggaagtcagc gacccgcgca cgcagctgat
     5401 cgagcctttg cgcagagtgc tgcagcgccc gatagccggg ctcgatgcgg cggcgcgcga
     5461 cgcggcgctc acctcgcagg aggtcacggt catggccgac aaggatctgc cgtacgaggt
     5521 cctcaagaag gttctggcga cgtgcacggc ggccgcgtac ggcaagatct cgctcgcggt
     5581 gctgcagaag gagaagccag tcagcggggt caagggcagc tagcggcctt gaatatggga
     5641 caacgtgacc gtcatttcac ctcaaggtaa cggcatgaat gcggaaatag cgcaagagca
     5701 gcagacgctg gaggaccagc ggacgccgga agatcagcag gcgcctaaca aagaggcgct
     5761 gcaggatcag gggacgctcc tcgcggaact cggcacgtcg caggagtcgg tcgatgccct
     5821 ggtgggcgag ctgcgctcgc tcgatgcgca gctcgaggcg ctggcggccg agcgcaacca
     5881 gcaccgcctg ctgcaccagg tctgcgacgc cctgggcgag ctcgggaagc ttggcggcgc
     5941 gcagttgttc tggggtactg cgcaagcggc gaagagcggc gaggagcagc tgggccgggt
     6001 gcacgaacgt gtggctgcct tcgagagccg cgtgggcgag ctggaggcgc gccgacagag
     6061 cacccgcgaa cagatcgacc aggagcagta tcgtgtcgga ctgatcgagg acgggctgtt
     6121 cgacgcgcag gaacaagagg agcggctgcg gcaggagtgg atcgtcgagc gggagatcag
     6181 cgggcttcac tcccgtgcg
//
LOCUS       DASPZD010000027         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1432663, whole genome shotgun sequence.
ACCESSION   DASPZD010000027 DASPZD010000000
VERSION     DASPZD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432663"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..156
                     /locus_tag="VEM33_00490"
                     /pseudo
     CDS             19..156
                     /locus_tag="VEM33_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451549.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            complement(205..474)
                     /locus_tag="VEM33_00495"
                     /pseudo
     CDS             complement(205..474)
                     /locus_tag="VEM33_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            729..1787
                     /locus_tag="VEM33_00500"
     CDS             729..1787
                     /locus_tag="VEM33_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYS50341.1"
                     /translation="MNPLRACLSVSGIFLLAALFGAASPVHAQGDASRATPPPGKALV
                     FVFRSDRQPVAAQVPVLVNLVRIGDLTDGTFAVATVNPGRTFLRIGEQALAVFTLEAV
                     ANRSYFVRVEAVSSVRPVRTEVRLVSETDGRRSLAQSRLLGAAPAIAAAPRAQPPAAP
                     VAAPAAAPRAQPSPPQPAPSAAGGDWTFALIATGGAFKLASANQTVAGLPSTFDATSK
                     PAAGIEGELRHRGGFALGGEVFYYKNDLLVNGTALKAQQQVIAVMANGKYYFRVADWF
                     HPFVGVGVGATDAVYSGDLTGNAGGAAFQGMAGMEFRFEHVGVLVQYKYLASTTGKSG
                     SDVKVGGGGVLAGLSIIF"
     gene            1962..2444
                     /locus_tag="VEM33_00505"
     CDS             1962..2444
                     /locus_tag="VEM33_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50342.1"
                     /translation="MLCEITVGPGYLKADLFNRQTAEEAREALAAIAAEARKHACSQI
                     LISVHASRPLFKVEQYGLPDYFKGLGEILKCRIALTGDSDELRLSQQYIELLARRNGV
                     NVRSFPSEEAALSWFRDRRWSLDRRLRQEPWNGQERRQRPSRRNLESIRADSVGRISV
                     "
     gene            complement(2481..>3423)
                     /locus_tag="VEM33_00510"
     CDS             complement(2481..>3423)
                     /locus_tag="VEM33_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50343.1"
                     /translation="LSGCGPAALAQATADYPHRAIRIVVPLAAGGNVDIVARTLADQV
                     SKSLGQPVLVENRPGASSLVGTQFVARSPADGYTVLAVANTFATVPLIVASPGYDPLK
                     DFTGITLTCLVPQVLVANPALPARSVKELVALARSQPGKISYASSGNGSTGHMAAELF
                     NRQAGVTMLHVPYKGNSQALIDVISGQVAMMFDQVSTSTPQIKAGKVRAIAVTSLARS
                     PLLPEVPTVDESGVPGYEDITFNGMVAPVGTPRAVLVRLNRAIAEAVSEPELHKRFIE
                     RGVELRASASPEEFTERIRAEFEKKARLAREAGIKPE"
BASE COUNT          573 a         1143 c         1106 g          601 t
ORIGIN      
        1 ctcacggcga cccttgcgat actggcgccg cacgccgccg ctgcggcgga cgcctacccc
       61 gccaagccgg tgcgcttcgt cgtcgccttc ccgccaggcg gcggcaccga catcatcgcc
      121 cgctcgatcg cgcagaagct cgccgagcgc agccggatag ccgcagtaga tcgcggagtg
      181 cagcaggatc tcgccgatct ccttcctcgt caccccgttg cggatcgcgc caccgagatg
      241 gagctcgagc tcctcggctt ttccgagcgc aaccagcatc gcgatattga gcatgctgcg
      301 gaccttgcgc ggcagccccg gacgcgtcca caccatgccc catgccgatt tggtcgccag
      361 caactgcatc ggcatcgaaa acgcgtcagc gttcttgatc tgctcctcca cgtacgcggc
      421 gcccagcacc tgcttgcgca atttcagacc ttgctcgaaa agcttgtctc tcatttcaac
      481 gctccccaaa gttcgaccct ccgaacgacc gtcggccgga tcgaattttc tatcgcccca
      541 ttgtctgata gagtgcgacg gtcacgataa tcgaaagaac agatcggtac aatgtctcac
      601 ctccagggtt atgggcagcg cgggcacctt ttcccggcct ccctcggacg ctacaatgaa
      661 gagcggatgt caacgatgat cctccgcggt ctcgcgaggg gagaagaaac aacgagggga
      721 ctccacccat gaacccgctg cgcgcatgcc tttcggtgag cggcatcttc ttgcttgccg
      781 cgctgtttgg tgccgcctcg cccgtgcacg cacaggggga cgcttcgcgg gcaaccccgc
      841 cgcccggcaa ggcgctggtg ttcgtttttc gcagcgaccg tcagcccgtg gccgcgcagg
      901 tgccggtgct cgtgaacctg gtgcgtatcg gcgacctgac ggacggcacc tttgccgtcg
      961 cgaccgtcaa tcccggaagg actttcctgc gcatcggcga acaggcgctg gccgtgttca
     1021 cgctcgaagc cgtggccaac cggagctatt tcgtgcgggt cgaggccgtc tccagcgtga
     1081 ggccggtgcg aaccgaggtg cgtctggtga gcgaaaccga tggccgccgc tcgctcgcgc
     1141 agagtcgcct cctcggcgcc gcgccggcga tcgccgccgc accgcgcgcc cagccaccgg
     1201 cagccccggt cgcggcgccg gccgcggcgc cgcgcgcgca gccgagcccc ccccagcctg
     1261 ccccgtccgc cgcgggtggt gactggacct tcgcgctcat cgcaacggga ggcgcgttca
     1321 agctggcgag cgccaaccag acggtcgccg gcctcccgag cactttcgac gccacgtcca
     1381 agccggcggc tggcatcgag ggggagctgc gccacagggg gggcttcgca ctgggcggcg
     1441 aggtcttcta ctacaagaac gatttgctcg tgaacggaac ggctcttaaa gcccagcagc
     1501 aagtgatcgc cgtcatggca aacggaaagt actacttccg cgtggcggac tggttccatc
     1561 ctttcgtggg ggtcggcgtg ggtgccaccg atgccgtcta cagcggcgac ttgacgggga
     1621 atgctggggg tgcggctttt cagggcatgg cgggcatgga attccggttc gagcacgtcg
     1681 gcgtgctggt gcagtacaag tatctcgcct ccacgaccgg gaaaagcggg agcgacgtca
     1741 aggtcggcgg cggcggcgtt ctcgccggac tcagcatcat tttttgagcc ccatcccgct
     1801 tcgcccgggc ggtcgcccga ttatgccatc ggcatagccc atgcggacta ccttgtgacg
     1861 cgatgtttca ttgcgtttgt aggccatgct accagcaggc agctgtttgg gagcggaagg
     1921 ggagccgatc ccgacgacac gacatctttc ggtgcactgc aatgctatgt gagatcaccg
     1981 tcgggccggg ttatctcaag gcggatctgt tcaaccggca gacggctgag gaagcgcgcg
     2041 aggctcttgc cgccattgcg gccgaggcgc gcaagcacgc gtgctcgcag atcctcatat
     2101 cggttcacgc ttcgagaccc ttgttcaaag tcgagcagta cgggctcccc gattacttca
     2161 aagggctggg cgagatattg aaatgccgaa ttgcgctgac gggcgactcc gacgagctga
     2221 gactctccca gcaatacatc gagttgcttg cgcgacgcaa cggggtcaac gttcggagct
     2281 ttcccagcga ggaagcggcg ctctcttggt tcagggaccg tcgatggtcg ctggaccggc
     2341 gcctgcgaca ggagccatgg aacggacaag agcgacgcca gcgcccgtcc cgccgcaacc
     2401 tggaaagcat ccgtgcggac tcggtcggac gcatttccgt ataacgggca gactcgaacc
     2461 cgcgaccgtt ttggccttcc ctactcgggt ttgattccag cctcgcgcgc gagccgggcc
     2521 ttcttctcga actccgccct gatgcgctcg gtaaactctt cgggcgaagc gctcgccctg
     2581 agctcgacgc cgcgctcgat gaaacgcttg tggagctcgg gctcgctcac ggcttcggca
     2641 atcgcccggt tgaggcggac cagaaccgcg cgaggtgtcc ccacgggcgc aaccattccg
     2701 ttgaaggtga tgtcctcgta gcccggcacg ccggactcgt ccacggtcgg gacctcggga
     2761 agaagcggcg agcgcgcgag actcgttacc gcgatcgccc gtaccttccc cgccttgatt
     2821 tgcggcgtgg acgtgctcac ttggtcgaac atcatcgcga cctggccgct gatcacgtcg
     2881 atgagcgcct gcgagttgcc cttgtagggg acgtgcagca tcgtcacgcc cgcctggcgg
     2941 ttgaagagct ccgccgccat gtggccggtc gagccattcc ccgagctcgc gtaggagatt
     3001 ttccccggct gcgatcgtgc gagcgcgacg agctccttca ccgaccgcgc ggggagcgcg
     3061 ggattggcga cgagcacctg gggcacgagg caggtgagcg tgatgccggt gaagtccttc
     3121 agcggatcgt agccgggact cgccacgatc aagggcaccg tggcgaaggt gttcgcgacc
     3181 gcgagcaccg tatagccgtc cgccggcgac ctcgcgacga actgcgtgcc cacgaggctc
     3241 gacgccccgg ggcggttctc gacgaggacg ggctggccca ggctcttcga aacctgatcc
     3301 gcgagcgtgc gcgcgacgat atcgacgttg ccgcccgcag cgagtggaac gacgatgcgg
     3361 atcgcgcgat gcggatagtc ggctgtcgct tgcgcgagcg cggccgggcc gcatcccgaa
     3421 agg
//
LOCUS       DASPZD010000028         5521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1314023, whole genome shotgun sequence.
ACCESSION   DASPZD010000028 DASPZD010000000
VERSION     DASPZD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314023"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VEM33_00515"
     CDS             <1..156
                     /locus_tag="VEM33_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HYS50344.1"
                     /translation="ETHEYTDMYPGMSKAARQEGFSEIADWFETLAKAERSHANRFQK
                     ALDALAD"
     gene            182..1510
                     /locus_tag="VEM33_00520"
     CDS             182..1510
                     /locus_tag="VEM33_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HYS50345.1"
                     /translation="MTFREGSLEAPTRHPVAWRLPDFYDEAKVLAELERIFGICHGCR
                     RCVNLCTAFPTLFELVDASATLEVDGVEKSQFWKVVDQCYLCDMCYMTKCPYVPPHPW
                     NVDFPHVMLRAKAVKFKKGEVRLRDRLLSSTDAIGRLAAIPVVAQVINAVNRNSASRA
                     LLEKAIGVHPERRLPPFSPGTFRGQAAASLSHEPRNGEHTPGKVAVFSTCYVKYNEPG
                     IGHDLLAVLDHNEIPYVIVEKEACCGMPKLELGDLEGVERLKALNVPRLVKLAREGYA
                     ILTAVPSCTLMFKSELPLLFPDDSDVKAVSEAMFDPFEYFVLRKRDGLLKTDFKTALG
                     KVSYHVPCHSRVQNMGQKTRETLQLVPGTTLNTVERCSGHDGTWGVKSEYYENSMKIG
                     RPVFRQMAGNEPDYISSDCPIAARHIEQGMGGTRAIKAHPLALLRKAYGI"
     gene            1531..2121
                     /locus_tag="VEM33_00525"
     CDS             1531..2121
                     /locus_tag="VEM33_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HYS50346.1"
                     /translation="MDAMPRIDRASLMTLETYARERPQFRAKVIAHKKDRTVHLGEHV
                     TLVFEDELTIRYQVQEMLRIERIFEEEGIQEELAAYNPLVPDGRNLKATMMIEYPDPV
                     ERARRLAELIGLEDKVWIQVKGHERVCAVADEDLERENEQKTSAVHFLRFELDEAMVQ
                     ALKKGAGLAIEIDHPRYTAAVEAPPAVRKALAKDLT"
     gene            complement(2138..3244)
                     /gene="proB"
                     /locus_tag="VEM33_00530"
     CDS             complement(2138..3244)
                     /gene="proB"
                     /locus_tag="VEM33_00530"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYS50347.1"
                     /translation="MREARRFVVKVGSTLVTNRGAGLDASAIARWAAQVAKLRQGGKE
                     FVLVSSGAIAEGVQRLGWTRRPHAMHELQAAAAVGQMGLVQVYESGFREHGLHTAQVL
                     LTHADLADRQRYLNARSTLLTLLGLGIIPVINENDTVVTDEIRFGDNDTLAALVTNLV
                     EADCLIILTDQQGLYTDDPRRNPEATLVREAQAGEARLEEIAGGTGSTLAKGGMLTKV
                     LAAKRAARSGAQTVIASGHEPDVLVRIAAGEAVGTLLTAQTTPLAARKQWLADHVQVG
                     GRLSIDAGAAKALRSGGKSLLPIGVKGVEGEFERGAVVACLSPEGAEIARGLVNYDAQ
                     ESRRIAGRASGEIEAILGYIDEPELIHRDNLVLL"
     gene            complement(3277..4260)
                     /gene="obgE"
                     /locus_tag="VEM33_00535"
                     /pseudo
     CDS             complement(3277..4260)
                     /gene="obgE"
                     /locus_tag="VEM33_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            complement(4326..4583)
                     /gene="rpmA"
                     /locus_tag="VEM33_00540"
     CDS             complement(4326..4583)
                     /gene="rpmA"
                     /locus_tag="VEM33_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYS50348.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKRYGGQIVAAGSIIIRQRGTR
                     VHPGDNVGVGKDHTLFAKVNGKIQFSFRGPDHKQTVSVVPA"
     gene            complement(4612..4926)
                     /gene="rplU"
                     /locus_tag="VEM33_00545"
     CDS             complement(4612..4926)
                     /gene="rplU"
                     /locus_tag="VEM33_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYS50349.1"
                     /translation="MYAVVRTGGKQYRVSTGEKLRVEKLAAAVGSELVLDEVLLVGDE
                     QALKVGTPVVKGASVKAKIVAHGLGEKVMIFKLRRRKNSKRLRGHRQAYTEIEVTGIA
                     QA"
     gene            5091..>5521
                     /locus_tag="VEM33_00550"
     CDS             5091..>5521
                     /locus_tag="VEM33_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYS50350.1"
                     /translation="MLAVDAMIRRRLESDVVLVRQIAEYIIAGGGKRLRPALVFLAAG
                     ATGYRGEHHVELAAVVEFIHTATLLHDDVVDESELRRGRKTAYAEFGNAASVLVGDFL
                     YSRAFQIMVAVRSMRVMEVLADATNAIAEGEVLQLLNAHNFS"
BASE COUNT          997 a         1764 c         1720 g         1040 t
ORIGIN      
        1 gagacccacg aatacaccga catgtatccc ggcatgtcga aggccgcgcg tcaggaaggt
       61 ttctccgaga tagccgactg gttcgagacc ctcgccaagg cggagcgctc gcacgccaac
      121 cgtttccaga aggcactcga cgcgctcgcc gactagaaaa ccggttccgt cgaatgttga
      181 gatgaccttt cgcgaaggga gcctggaagc tccgacacgc catccggttg cgtggcggct
      241 gccggatttc tatgacgaag cgaaagtgct cgcggagctc gagcgcatat tcggcatctg
      301 ccacggatgc cggcgctgcg tcaacctgtg cacggcgttt ccgacgctct tcgaactcgt
      361 ggacgcgagt gcgacgctcg aagtcgacgg cgtggagaaa tcgcagttct ggaaggtcgt
      421 ggaccagtgc tatctctgcg acatgtgcta catgacgaaa tgcccgtacg tgccgccgca
      481 tccctggaac gtcgattttc cgcacgtcat gctgcgagcc aaggcggtga agttcaagaa
      541 gggcgaggtg cggctgcggg accgcctgct ttcctccacg gacgccatcg gcaggcttgc
      601 cgcgatcccg gtcgtcgcgc aagtgatcaa cgccgtcaac cgcaactccg cttcgcgcgc
      661 gctcttggaa aaggcgatcg gcgtccaccc tgagcgcagg ctgccgccct tcagtccggg
      721 aacgtttcgc ggacaggctg cggccagcct gagccatgag ccgcgcaacg gtgagcacac
      781 cccgggaaaa gtggcggtat tctcgacctg ctacgtgaag tacaacgagc cgggcatcgg
      841 ccacgatctg ctcgcggttc tcgatcacaa cgagattcct tacgtgatcg tggagaaaga
      901 agcctgctgc ggcatgccca agctcgagct gggcgatctc gaaggcgttg agcgcctgaa
      961 ggcgctcaac gttccgcgac tcgtcaagct cgcgcgcgag ggctacgcga ttctcaccgc
     1021 ggtgccctcg tgcacgctga tgttcaagag cgagctgccg ctgctgtttc cggacgattc
     1081 cgacgtcaag gcggtaagcg aggcgatgtt cgacccgttc gagtacttcg tcctgcgcaa
     1141 gcgtgacggc ctgctcaaaa ccgacttcaa gaccgcgctc ggaaaagtgt cctaccacgt
     1201 gccgtgccat tcccgcgtgc agaacatggg acagaagacc cgcgaaacac tgcagctcgt
     1261 accggggacg acgctcaata cggtggagcg ttgctccggc cacgatggaa cctggggcgt
     1321 caagtccgag tactacgaaa actccatgaa gatcgggcga cccgtgttcc ggcagatggc
     1381 cggcaacgag cccgattaca tcagttcgga ctgcccgatc gccgcgcgcc atatagagca
     1441 gggcatgggc gggacccggg ctatcaaggc gcaccccctg gcgctccttc ggaaagccta
     1501 cggaatttga acctcaacga cgatgtgacg atggacgcca tgcctagaat cgaccgcgcg
     1561 agcctcatga ccctcgaaac ctacgcgcgc gagcgcccgc agttccgcgc gaaggtcatt
     1621 gcgcacaaga aggaccgcac ggttcacctg ggcgagcacg tcactctcgt cttcgaggac
     1681 gaactcacta tccgttacca ggtgcaggag atgttgcgca tcgagcggat cttcgaggaa
     1741 gagggtatcc aggaggagct cgctgcctac aacccgctcg tcccggacgg ccgcaatctc
     1801 aaggcaacca tgatgatcga gtacccggat ccggtggagc gcgcgaggcg gctcgcggag
     1861 ctgatcgggc tcgaggacaa ggtatggatc caggtcaaag ggcatgagcg ggtctgtgcc
     1921 gtcgccgacg aggatctcga gcgggagaac gagcagaaaa cctcggcggt gcactttttg
     1981 cgcttcgagc tcgacgaggc aatggtccaa gcgctcaaaa aaggcgcggg actcgcgatc
     2041 gagatcgatc atccccgcta tacggccgcg gtggaagcgc ccccggccgt gcgcaaggct
     2101 cttgccaaag atctgacgtg aggcgattgc gctcctgtta cagcagcacc aagttatctc
     2161 ggtggatcag ttccggctcg tcgatatatc cgagtatggc ctcgatctcg cctgacgccc
     2221 ggccggcaat gcgccgggat tcttgcgcgt cgtaattgac cagtccacgc gcgatctcgg
     2281 cgccctccgg cgagaggcac gcgactaccg cgcctcgctc gaattcaccc tccacgccct
     2341 tgaccccgat cggcagcagg ctcttgccac ctgagcgtaa ggccttggcg gcaccggcgt
     2401 cgatgctgag ccttcctccg acctgcacgt gatcagcgag ccactgcttg cgtgcggcga
     2461 gtggcgtcgt ctgcgcggtg agcagggtgc ccacggcttc tccggcggcg atcctgacca
     2521 gcacatcggg ctcgtgtccc gaagcgatca ccgtttgcgc tcccgagcgg gccgcgcgct
     2581 tggctgcgag caccttggtg agcatgcccc cctttgcgag agtggagccg gttccgcctg
     2641 ctatttcctc gagcctcgcc tcgccggcct gcgcttcgcg caccagcgtt gcctccggat
     2701 tgcgccgggg atcgtcggtg tagagcccct gttggtcggt gaggatgatc agacagtcgg
     2761 cttcgacaag attcgtcacc aatgcggcaa gcgtgtcgtt gtcgccgaac cggatttcgt
     2821 cggtaacgac ggtgtcgttc tcgttgatca cgggaatgat tccgagcccg agcagtgtca
     2881 gcaaggtgga gcgggcattc aagtagcgct gcctgtcggc gagatcggca tgggtgagga
     2941 gtacctgcgc cgtgtgcagg ccatgctccc ggaagcccga ctcgtatacc tgcacgagtc
     3001 ccatctgccc gactgcggcg gcggcctgca gctcgtgcat ggcgtgcgga cgccgtgtcc
     3061 aacccaggcg ctgcacgcct tcggcgatcg cgccgctcga caccaggacg aactccttgc
     3121 cgccttggcg aagcttcgcg acttgcgctg cccagcgcgc gatggccgac gcgtcgagcc
     3181 cagcgccccg gttggtgacg agggtgctgc cgaccttgac gacgaaacgg cgtgcctctc
     3241 tcatgccgcc tttccggttt ccaagaattt catcgccgcg aaactgagtt cgcggcaacc
     3301 ctcgccggtg agcgccgata tgcaaaagct cgggccgcgc cagcgaagcc tgcgcacaag
     3361 gctggcggct acggtcttgc gctcctccgc ggggagcata tccattttgt tcagcaccag
     3421 ccagcgcggc tttcggtgca gcgcgggatc gaatttcttc agttcggctg caatggatcg
     3481 tgcatcgcga gccggatcgg cggccggatc aaagggtgcc acatcgacga tgtgcaggag
     3541 cagccgcgta cgcgcgaggt gcctgaggaa ctggtgcccc aagccggcac cttcagctgc
     3601 tccttcgatg agccccggga tatcggcgat cacgaagctg cgatcgatcc cggcgcgaac
     3661 cactccgaga tgcggatgta aagttgtaaa cgggtagtcg gcaaccctgg ggcgggcagc
     3721 cgagacggcg cgaatgaagg tggattttcc ggcattgggt aggcccagca atccaacatc
     3781 ggcaagcacc ttcaactcga gcttgagggt ccgatgctcg cccggctcgc cgggtgtcgc
     3841 cttgcggggg gtgcggttcg tgctcgattt gaaattgaca ttgccgaggc cccctctgcc
     3901 tccgcgcgcg agcaaagcga tctgctcgtg cacggcgagg tcggcgaccg tttctcccgt
     3961 ctccgcgtcc atgatcacgg tgcccgcggg cacgcgcagc acgacgtcat cggcaccttt
     4021 cccgctgcac tgggcgccgc ggccgcgctc gccgtctcgc gcgcggtgaa tgcgcgcgaa
     4081 acgatattcg acaagggtat tgacgtttcg gtcggcgacc gcgtagacgc cgccgccgct
     4141 cccgccgtcc ccgccatcgg gccctccgcg cggcacgttc ttttcgcgcc tgaagctcac
     4201 gatgccgtcg ccgccatcgc cggcgtggac ctcgatgcgc gcttcgtcga cgaatttcat
     4261 ggcgcctgtg gaaacaaaaa agccctaccg cgtggatagg gctttcggga aaagcagctt
     4321 tgcttctaag ccgggaccac gctgaccgtt tgtttgtggt ccgggcccct gaagctgaat
     4381 tggatcttgc cgttcacctt tgcgaaaagg gtatggtcct tcccgacccc gacgttgtcc
     4441 ccggggtgaa cgcgagtacc gcgctggcga atgatgatgc tgcccgcggc tacgatctgg
     4501 ccgccgtagc gtttgacacc gagacgcttt gattgcgagt cgcggccgtt gcgggaactg
     4561 ccgcctgctt ttttatgtgc catggatcgc ctttctgctc aaactgctgt cctacgcctg
     4621 cgcaatacct gtgacctcga tttcggtgta ggcctgacga tggcccctga gccttttcga
     4681 gttcttgcgc cggcgcagct tgaaaatcat cactttctcg ccgaggccgt gagcgacgat
     4741 cttcgccttc accgaggcgc ctttcaccac cggagttccg actttgagcg cctgttcgtc
     4801 accgacaagg aggacttcgt cgaggacaag ctcactgccc accgcggcgg cgagcttctc
     4861 gaccctcagt ttctccccgg tggaaacgcg atattgttta cccccggttc tgacgacggc
     4921 gtacatgctg gtctccgcgg aaaggatttg aaaaacacga aattatacat acctttagcg
     4981 ggtgcggtca aagaaaatat gctatcctcc tgacccgccg ttgagtcgat tccgcagtgc
     5041 gataatcccc tttgcagccc gaaccccttc gcaaccgcat cgccgcagac atgcttgcgg
     5101 tggatgccat gatccggcgt cggctcgagt ccgacgtggt gctcgtacgc cagatcgcgg
     5161 agtacatcat cgccggaggc ggcaagcgcc tgcgcccggc gctcgttttc cttgccgccg
     5221 gggcgaccgg ctatcgcggc gagcatcatg tggaactcgc agccgtcgtg gagttcattc
     5281 atacggccac gctcctgcat gacgacgtcg tcgacgaatc cgaactgcgg cgcggccgca
     5341 agacggccta tgccgagttc ggcaacgcgg cgagcgtgct cgtgggggac ttcctgtact
     5401 cccgcgcttt ccagatcatg gtagcggtgc gaagcatgcg ggtcatggag gtgctggcgg
     5461 acgccaccaa tgcgattgcc gagggcgaag tgctgcagct cctgaacgct cacaatttct
     5521 c
//
LOCUS       DASPZD010000029         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1669906, whole genome shotgun sequence.
ACCESSION   DASPZD010000029 DASPZD010000000
VERSION     DASPZD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669906"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VEM33_00555"
     CDS             <1..1214
                     /locus_tag="VEM33_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYS50351.1"
                     /translation="ETPSVRLGIVLGEAPAGFLSLADLISREPKKRPEDLRKLKIDPT
                     DPAIFQLSGGTTGVPKLIPRTHNDYAYNSRIAVKVCGVTGDSVLLLALPIGHNLPLAC
                     PGLQGTMLQGGKVVLSASTKPEDLFGLIERHRVTHLKVVPALLIRLINDPAIGKYDLS
                     SLHVIQSGGQRMQPEVRARTKGLIPSVTVQENFGMSEGMLFFVRLDDPEDVRMDTCGR
                     PLSPDDEVRLVDEEGREVARGEVGELTCRGPYTLRGYYGVPEYNARQFTPEGFYRSGD
                     LMRLHPSGNYVVEGRKKDLINRGGEKISAEEIENLILGHPAVQNIACVPMPDPHLGEK
                     MCACVIPKKGRSLSLKELVEFLKDREIAKFKLPERLEIVSDFPVSAFGKVSKKALVEM
                     VTAKLEREKAA"
     gene            1211..2053
                     /locus_tag="VEM33_00560"
     CDS             1211..2053
                     /locus_tag="VEM33_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50352.1"
                     /translation="MRIIDLHCYPGTKEWIACQGPYVDALAKYWNRGWTAKTEDEVIR
                     EFTDAGVEALLVALDLETTIKTPPVTNEYVHAMWKRHPKRVIQAWGALEPAKGKAAMA
                     QARKAVKELGLIGFHFHPIMQRFAVNDRRYYPLFELIDELKAAVMIDVGTTGMGAGMP
                     GGMGARIRHAHPSAIDDLAADFPNLRIIMAHPGWPWVDETTAVALHKGNVYWEMSGWA
                     PKYFPAQLKVDMRARLQDKIMFGSDYPSLPYARILSEWRELGYPDAVMEKIFHGNAER
                     ALAL"
     gene            2063..2830
                     /locus_tag="VEM33_00565"
     CDS             2063..2830
                     /locus_tag="VEM33_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYS50353.1"
                     /translation="MPTPSTKRAALVTGAASGIGRAAALALARAGYDVAINYSRSEKK
                     ANAVAQEAGRSGVRALLCRCDVADEAGVRAMLKAVGDQFGRLDVLINNAGTTTEVKPK
                     DFDALTVEEWDRVFAVNVRGLFLVTRAALPMLRASKGCIVNTASIVGLRPGPQPLPYA
                     ASKAAVVNLTRTLAYNLGPEIRVNAVAPGWMEGDWMQRMLKDKYEELMERRAKQTPLK
                     RCVTAEDVAETMMSLVQSNRFVTGEVIVIDGGFSSST"
     gene            2896..3195
                     /locus_tag="VEM33_00570"
     CDS             2896..3195
                     /locus_tag="VEM33_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYS50354.1"
                     /translation="MNEPVMKETLSSKALTAEELKLAQLIVSALNLETQAAEIDPTAP
                     LYGEGMGLDSIDILEVALAVSKAYGVKLRSDDDLNRKIFNSLRSLNGHIQQHRAK"
     gene            3225..>3709
                     /locus_tag="VEM33_00575"
     CDS             3225..>3709
                     /locus_tag="VEM33_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HYS50355.1"
                     /translation="MADIPLIRNFDAGAVFAFRDRRPLRVEEFLAEAHWLAGLIPDRQ
                     YVVNMCSDRYRFAAGLVAALLRHQVSLLPPNSTDDFLNRLSLEYPGAYCLGDGTAQIS
                     QLETFHYPQPTGEAASAASSRHIPEEQVAAISFTSGSTGRPRSHPKSWGALARSGQAA
                     F"
BASE COUNT          737 a         1162 c         1197 g          613 t
ORIGIN      
        1 aggaaacgcc atcggtcagg ctgggaatcg tgcttggcga ggcgcccgcc gggtttctct
       61 ctctcgcaga cctgatctcc cgcgagccga agaagcggcc tgaggatctg cgcaagctca
      121 agatcgatcc gaccgacccg gcgatattcc agctctcggg cggcacgacc ggcgtgccca
      181 agctgattcc gcgcacgcac aacgactacg cctacaactc caggatcgcg gtgaaggtct
      241 gcggggtcac gggtgactcg gtgctgctcc ttgccttgcc catcgggcac aatctgccgc
      301 tcgcctgtcc gggactgcag gggaccatgc tgcaaggcgg aaaggtggtg ctttcggcga
      361 gcaccaagcc tgaggatctg ttcggactga tcgagcggca tcgcgtcact cacctgaaag
      421 ttgtgccggc gctcctcatt cggctcatca acgatccggc cattggaaaa tacgacctgt
      481 cctcgctgca cgtcatccag agcggcggac agcgtatgca gcccgaggtg cgcgcgcgca
      541 ccaaggggct catccccagc gtcaccgtgc aggagaattt cggcatgtcc gaaggcatgc
      601 tctttttcgt gcgcctcgac gacccggagg acgtgcgcat ggatacctgc ggccggccgc
      661 tgtcgcccga cgacgaggtc aggctggtcg acgaggaggg cagggaggtc gcccgcggcg
      721 aagtcggcga gctcacctgc cgcgggccct acaccctgcg cggctactac ggcgttcccg
      781 aatacaacgc gcggcagttt acgcccgagg gcttctaccg gtccggcgac ctgatgcgcc
      841 tgcacccttc cggaaactac gtggtcgaag gcaggaagaa ggaccttatc aaccgcggcg
      901 gcgagaagat cagcgccgag gagatcgaga acctgatcct cgggcacccc gcggtgcaaa
      961 acatcgcctg cgtgccgatg ccggacccgc acttgggcga gaagatgtgc gcttgcgtga
     1021 tccccaagaa aggccgtagc ctgagcttga aggagctggt cgagttcctc aaggacaggg
     1081 agatcgccaa gttcaagctt ccggagcggc tcgaaatcgt gtcggacttc ccggtgtctg
     1141 ctttcggcaa ggtgtcgaaa aaagcgctgg tcgagatggt cacggcgaag ctggagcggg
     1201 agaaagccgc gtgagaatca tcgatctgca ctgctacccc ggcaccaagg aatggatcgc
     1261 gtgccagggc ccttacgtgg acgcgctcgc caagtactgg aaccgcggct ggaccgcgaa
     1321 gaccgaggac gaggtgatcc gcgaattcac ggacgccggg gtcgaggccc tgctggtcgc
     1381 actcgacctc gaaacgacga tcaagacgcc gcccgtcacc aacgaatacg tccacgcgat
     1441 gtggaagcgc caccccaagc gcgtcatcca ggcctggggc gcgctcgaac cggcgaaagg
     1501 caaggccgcg atggcgcagg cgagaaaggc ggttaaggag ctcgggctta tcggctttca
     1561 cttccatccg atcatgcagc gcttcgcggt gaacgaccgg cgctactatc ccttgttcga
     1621 gctcatcgac gaactcaagg ccgcggtcat gatcgacgtc ggcacgaccg gaatgggcgc
     1681 cggcatgccc ggcggaatgg gggcgaggat ccggcacgcg catccttccg ccatcgacga
     1741 tctagcggcg gattttccga acctgaggat catcatggcg catcccggat ggccctgggt
     1801 ggacgagacc acagcggtcg cgctgcacaa gggaaacgtc tactgggaga tgtcgggttg
     1861 ggcgccgaaa tattttccgg cccagctgaa ggtggacatg cgtgcgaggc tgcaggacaa
     1921 gatcatgttc ggcagcgact accccagcct gccttacgcg cgcatcctct cggagtggcg
     1981 cgagctcggc taccccgacg ccgtaatgga gaagatcttc cacgggaacg ccgagcgcgc
     2041 tctcgcactg tagacccaac gcgtgcccac gccctcgaca aaacgcgcag cgctggtgac
     2101 cggagcggca agcggcatcg gccgcgcggc ggcgctcgcc ctcgcgcgcg ccggctacga
     2161 cgtcgcgatc aactacagcc gcagcgagaa gaaggccaat gcggtcgcac aggaagccgg
     2221 ccgcagcgga gtgcgcgcgc tgctttgccg ctgcgacgtc gccgacgagg ccggcgtgcg
     2281 cgcaatgctg aaggcggtcg gggaccagtt cggccgcctc gacgtgctca tcaacaatgc
     2341 gggcacgacc accgaggtca aacccaagga tttcgacgcg ttgacggtgg aggaatggga
     2401 ccgcgtgttt gcggtcaacg tcaggggctt gttcctcgtc acgcgagccg ctctgccgat
     2461 gctgcgcgca tcgaaaggct gcatcgtcaa taccgcgagc atcgtcggcc ttcggccggg
     2521 gccgcagccg ctgccttacg cggcgagcaa ggctgcggta gtcaatctga ccaggactct
     2581 cgcgtacaac ctgggtccgg aaatccgagt gaacgccgtc gcaccgggct ggatggaagg
     2641 cgactggatg cagcggatgc tgaaggacaa atacgaagag ctcatggagc gccgtgccaa
     2701 gcagacgccc ttgaagcgct gtgtcaccgc ggaggacgtg gccgaaacaa tgatgtcgct
     2761 cgtccagtcg aaccgcttcg ttaccggcga ggtgatcgtg atcgacggcg gtttttccag
     2821 ctcgacctga gctgacggcg acccgagaac ccaggagcta taatggcgat cgcatcacgg
     2881 gaggaaacag ggaatatgaa cgagccggtg atgaaagaga ctctgtcgag caaggcactc
     2941 acagccgaag agctcaagct cgcccagttg atcgtctcgg cgctcaatct cgagacgcaa
     3001 gccgcggaaa tcgatccgac cgccccgttg tacggggagg gcatggggct ggactcgatc
     3061 gacattctgg aagtcgccct ggcggtctcg aaagcctacg gcgtcaagct gcgctccgac
     3121 gacgacctca atcgcaagat cttcaattcg ctgcgcagtc tgaacgggca cattcagcaa
     3181 catcgggcta aatagagcgg cagcttccag ccggcgggtg cgtcatggcc gatattccgc
     3241 tgattcgcaa tttcgacgcc ggagcggttt tcgcttttcg ggacaggcgg cccctccgcg
     3301 tcgaagagtt cctcgccgaa gcccactggc tggccggtct gattccggat cgacaatacg
     3361 tagtgaatat gtgctcggac cgctatcgat ttgccgccgg cctcgtcgca gcgctgttgc
     3421 gccaccaggt cagcttgttg ccgcccaata gcaccgacga ttttctgaac cgcttgagtc
     3481 tcgagtatcc gggtgcgtac tgtctgggcg acggaaccgc ccagatatcg cagctcgaga
     3541 ccttccacta tccgcagccg acgggcgaag ccgcgtccgc agcttcctct cggcacattc
     3601 cggaggaaca ggtggcagcc atcagcttca cctcgggctc gaccgggcgg ccgagatctc
     3661 accccaaatc atggggggca ttggcgagaa gcggacaagc ggcgttcga
//
LOCUS       DASPZD010000030         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1325230, whole genome shotgun sequence.
ACCESSION   DASPZD010000030 DASPZD010000000
VERSION     DASPZD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325230"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEM33_00580"
     CDS             <1..448
                     /locus_tag="VEM33_00580"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYS50356.1"
                     /translation="APLFSLPDADMETVDLGEFRGKKHVVLYFYPKDGTPNCTLEATD
                     FSDHEEEFSRHDCVVLGVSPDDCLRHADFRDQHGVSIRLLADTESEVCRKYGVVHEKE
                     ANGSTKSCITRSTFVIDKEGTVRHAFYGVNPRGHAAQVLDLVRSLR"
     gene            480..971
                     /locus_tag="VEM33_00585"
     CDS             480..971
                     /locus_tag="VEM33_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYS50357.1"
                     /translation="MIIAKDTVVSLDVELSDIWGKLIQRSEEPLQYLHGGYGNIFPVA
                     EAALEGKKVKDRVEVRLEPEDAFGDYHENLLRVEPRSRFPEVLEVGMRFEGAADGDDA
                     NLIFTVTDMAEDKVVLDANHALAGMALKLSCTVVGVRAATDVEIRNGSADDPESVILR
                     ILP"
     gene            1060..2559
                     /locus_tag="VEM33_00590"
     CDS             1060..2559
                     /locus_tag="VEM33_00590"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HYS50358.1"
                     /translation="MSGMLGVVSYGLGTVIGFGLLAFLVIVFIQDITQKKHTVLRNYP
                     VIGHLRYFLEKQGEYFRQYFFLNDREELPFNRATRGWVYRLAKAEGGVIGFGSTNNTN
                     EPGSILFVNHPYPVLEEDRLPTPPLTIGEDFCREPFLGRSVVNISGMSFGAISEPAVR
                     ALSRGAALAGCWMDTGEGGLSSFHLEGGCDVIMQIGTANYGVRAADGSFSRERAKEVA
                     AFPQVKAFEIKLSQGAKPGKGGVLPAAKVTQQIAGIRGIPPLQDSISPNRHRDVANVD
                     QLLDKIALVRDLTGKPVGVKTAIGGWQFMNELCDAIHRRGLEYAPDFLAIDGGEGGSG
                     AAPQALMDHMALPIAEALPRVIDSLIESGLRNRIRVIAAGRLVTSAKAAWALCVGADF
                     VNTARGFMFSLGCIQALRCHQNTCPTGVTTHNPRLQRGLVVEEKHLRVANYAMGMNKE
                     IDMIAHCCGCRHARDLKREHVRIVQTAGHSAALNILYPYPEPNTKRKAA"
BASE COUNT          500 a          899 c          866 g          489 t
ORIGIN      
        1 ggcgccgctt ttctcgcttc ccgacgcaga catggaaacg gtggacctcg gcgagttccg
       61 gggaaagaaa cacgtcgtgc tctatttcta tcccaaagac ggcacgccga actgcacgct
      121 ggaggccacc gatttttccg atcacgaaga ggagttttcc cgccacgact gcgtggtgct
      181 cggggtgagt ccggacgact gccttcgcca cgccgacttc cgcgaccagc acggcgtttc
      241 gatccggctg ctcgcggaca ccgagagcga agtctgcagg aaatacgggg tggtgcacga
      301 gaaggaagcc aacggatcca cgaaatcctg catcacgcgc tccactttcg tcatcgacaa
      361 ggagggcacg gtgaggcacg cattctacgg cgtcaatccc cgcgggcacg ccgcccaggt
      421 gctggacctc gtcagatcgc tgcgttgacc ggagtcgggc cccgagcccg cccgagcact
      481 tgatcatcgc aaaggacacg gtcgtctcgc tcgacgtcga gctgtccgac atctggggca
      541 aactgatcca gcgttccgag gaacccctcc agtacctgca cggcggctac gggaacatat
      601 ttccggtggc ggaggccgcc ctcgaaggaa agaaagtgaa ggaccgcgtc gaagtgcgtc
      661 tcgagcccga ggatgctttc ggcgactacc acgaaaacct tctcagagtc gagccgcgca
      721 gccgctttcc cgaggtcctc gaggtcggga tgcgcttcga gggggctgcg gatggcgacg
      781 acgcgaacct gatcttcacg gtcaccgaca tggccgagga caaggtcgtg ctcgacgcca
      841 accatgcgct tgcaggcatg gcgctcaagt tgtcctgcac cgtcgtcggc gtgcgcgcgg
      901 ccaccgacgt cgagattcga aacggctcgg ccgacgatcc cgaatcggtg atccttcgca
      961 ttctgccctg aacttccccg gcgccgcgtg ttttccccta tcatttgacg cgggctccgc
     1021 accgcgttcc tgcggaaatc aaccggagag gaggcaagca tgtccggcat gctgggcgtc
     1081 gtctcgtacg gcctcgggac tgtgatcggg ttcggcttgc ttgcgttcct cgtgatcgtc
     1141 ttcatccagg acatcacgca aaagaagcac acagttcttc gcaactatcc ggtcatcggg
     1201 cacctgcgct attttctgga gaagcagggc gagtacttca ggcagtattt cttcctgaac
     1261 gaccgcgaag agctgccctt caaccgcgcc actcgcggct gggtctaccg cctggcgaag
     1321 gccgaaggcg gcgtgatcgg cttcggctcg accaacaaca ccaacgagcc cggctcgatc
     1381 ctgttcgtca accatcctta tccggtgctg gaggaggacc gcctgcccac gccgccgctc
     1441 acgatcggcg aggacttctg cagggagccg ttcctcggaa gatcggtggt gaacatcagc
     1501 ggcatgagct tcggggcgat ctcggagccc gcggtgcgcg ccttgtcgcg gggcgcggcg
     1561 ctcgccggct gctggatgga cacgggcgaa ggagggcttt cgtcattcca tctggagggc
     1621 ggctgcgacg tcatcatgca gatcgggacc gccaactacg gcgtgcgcgc cgcggacgga
     1681 agcttttccc gggagcgggc gaaggaggtc gcggcgtttc cgcaggtgaa ggcgttcgag
     1741 atcaagctct cgcagggcgc gaagcccggc aaggggggcg tgctgccggc ggcgaaagtc
     1801 acccagcaga tcgcgggcat ccgcggcatt ccccctttgc aggattcgat cagcccgaac
     1861 cgtcaccgcg atgtcgccaa cgtcgatcag ctgctcgaca agatcgctct cgtccgcgac
     1921 ctcaccggca agcccgtggg cgtgaagacg gcgatcggcg gctggcagtt catgaacgag
     1981 ctctgcgatg cgatccacag gcgcggcctc gagtacgcgc cggattttct cgccatcgac
     2041 ggcggagaag gcggctcggg cgcggcgccc caggcgctga tggatcacat ggcgctgccg
     2101 atcgccgagg ccctaccgcg cgtcatcgac tcgctgatcg agtcgggtct gcgcaaccgc
     2161 atccgcgtca tcgccgcggg caggctcgtc acttccgcga aagccgcgtg ggcgctgtgc
     2221 gtcggcgcag atttcgtcaa cactgcgcgc ggattcatgt tctcgctcgg ctgcatccag
     2281 gcgctgcgct gccaccagaa cacctgtccc acgggcgtga ccacgcataa cccccgcctg
     2341 cagcgcggac tcgtggtcga ggagaagcac ctccgtgtcg caaactacgc gatgggcatg
     2401 aacaaggaga tcgacatgat cgcgcactgc tgcggctgcc ggcatgcgcg cgacctgaag
     2461 cgcgagcacg tgcgcatcgt gcaaaccgcg ggccacagcg cggctctcaa cattctttat
     2521 ccctaccccg agccgaacac caagcgcaag gccgcctgat cccttgcatg gcggtactat
     2581 ttcctgtagt ccggggaagc cttgagctgg gcgctgcacc gatgtgctag attgctcgca
     2641 cctttacctc tcgtccagga gatcatgatg aatctcaaac gcggcctcgt cgccgcatcg
     2701 tttcttgcgg tgtcgctgcc cgttgtcgcg gcggacatca accagatcca gact
//
LOCUS       DASPZD010000031         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_705745, whole genome shotgun sequence.
ACCESSION   DASPZD010000031 DASPZD010000000
VERSION     DASPZD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705745"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..76)
                     /locus_tag="VEM33_00595"
     CDS             complement(<1..76)
                     /locus_tag="VEM33_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50359.1"
                     /translation="MLVHGAWHGGWCWKRVAPALRAAGH"
     gene            complement(178..1200)
                     /locus_tag="VEM33_00600"
     CDS             complement(178..1200)
                     /locus_tag="VEM33_00600"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HYS50360.1"
                     /translation="MQLKTTIAGSLPKPAWLAEPKKLWAPWLLEGDLLEEGKRDAMRL
                     MLADQEAAGIDIVTDGEQTRRHFVTTFIESLKGVDFEHKRTVRIRNRYDADVPVVVAP
                     VGRGKPVFVEDLRFLRGQTKRPIKFSLPGPMTMVDTLYDNHYRSREKLAREFAAILNQ
                     EARELERAGADVIQFDEPAFNVYMDEVAAWGIATLEAAAAGLKCKTAVHICYGYGIKA
                     NIDWKKTLGSEWRQYESTFPLLARSRIDQISLECANSHVPLELLALLGGKDVLVGAID
                     VANDAVEKPEQVAATIRAAMRFVPPEKLYPCTNCGMVPLARDVALGKLRALASGAAVV
                     RREIGS"
     gene            complement(1207..2043)
                     /locus_tag="VEM33_00605"
     CDS             complement(1207..2043)
                     /locus_tag="VEM33_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate 1,2-dioxygenase"
                     /protein_id="HYS50361.1"
                     /translation="MARLVGVFASSHAPLIVRGWKDVKPANQEQLTSSLTELGRRIKA
                     ARPDVLVEISPDHWVNFFINNLPSICVGMGEEHEGPAEPWMKDCPFKKIAGHPKLANH
                     ILQTALERDFEPSVSHHLTLDHGFCIPLWRGGLDPLPPIVPIIFNDLEPPYPSVRRCL
                     QWGTMLAEAVRTYPGDIRVAILATGGLSHSIGEPTMGEIDEPFDRECIGHFEKGDERP
                     LIDFLNERLPTIGNGAAEIRNWVAAHGAAGKRGFELIGYCNCPEVYVGCGFASWNVAA
                     RA"
     gene            complement(2036..2317)
                     /locus_tag="VEM33_00610"
     CDS             complement(2036..2317)
                     /locus_tag="VEM33_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50362.1"
                     /translation="MRDYWLSKLFFDLQSSAPLGEEYRVNRKKILDRYPLKPEVRTAV
                     ERDDVAFLAPRVNPYLLRFYFVVAGMSEADFLKKVRTAGADAAARSSHG"
     gene            complement(2345..3088)
                     /locus_tag="VEM33_00615"
     CDS             complement(2345..3088)
                     /locus_tag="VEM33_00615"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYS50363.1"
                     /translation="MLAALLSALFLLAVTHAQPSEAQDSRAAARAQMVAGIAALALET
                     GAETGRPRFGEAVMAAMGKVPRHRFVPVLQDIFAYDNRPLPIGEGQTISQPYIVALMT
                     DLLDPKPTDTVLEVGTGSGYQAAVLAELVAKVYTIEIVEALGLRARQVLGELGYRNVE
                     VRVGDGYGGWPAAAPFDAIIVTAAPAAVPQPLVDQLKPGGRMVIPVGAPSDVQHLLLM
                     EKQSDGRTTTRRALPVRFVPLTRDRGTKQ"
     gene            complement(3102..>3420)
                     /locus_tag="VEM33_00620"
     CDS             complement(3102..>3420)
                     /locus_tag="VEM33_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS50364.1"
                     /translation="WGNQISGGEQQMLAIGRALMGNPTLLLMDEPLEGLAPIIVEVLL
                     QALQRLIQEDSLAIVLVEQHAKVALGITQRALVLNRGRIAYSGPSADLLADPQRLTSL
                     VVA"
BASE COUNT          528 a         1125 c         1160 g          607 t
ORIGIN      
        1 catggccggc cgcccgcagc gccggcgcca ccctcttcca gcaccacccg ccgtgccagg
       61 cgccgtgtac gagcacgtac gtggacttgc gcttggttgc gccgaagcgc ggcgaagcgc
      121 gcgcgcgcga gccgggcttg cggcgatgct tcagtttttt tgcgcgcggc acgtcggtca
      181 gcttccgatc tcccgccgca cgaccgcggc ccccgatgcg agggccctca gcttgccgag
      241 cgcgacatcg cgcgcgagcg gcaccatgcc gcagttggtg cagggataga gcttctccgg
      301 cggtacgaat cgcatcgccg cgcggatcgt cgcggcgact tgctcgggct tctctacggc
      361 gtcgttcgcg acgtcgatgg ctccgaccag cacgtccttg ccgccgagaa gcgcaagcag
      421 ctcgagcggc acgtgcgaat tcgcgcactc cagggagatc tggtcgatcc tcgatcgcgc
      481 gagcagggga aaggtggact cgtattggcg ccattcggaa ccgagcgtct tcttccagtc
      541 gatgttcgcc ttgattccgt atccgtaaca gatgtgcacg gccgtcttgc atttgaggcc
      601 cgccgctgcc gcttccagcg tggctatccc ccaggctgcg acttcgtcca tgtagacgtt
      661 gaaggccggc tcgtcgaact ggatcacgtc ggctcccgcc cgctcgagct cgcgcgcctc
      721 ctggttgagg atcgcggcga attcgcgcgc cagcttctcg cggctgcggt agtggttgtc
      781 gtagagcgtg tccaccatcg tcatcgggcc ggggagggag aatttgatcg ggcgcttcgt
      841 ctgcccccgc aggaagcgaa ggtcctcgac gaacaccggc ttgccgcgcc ccacgggcgc
      901 caccaccacc ggcacgtccg cgtcgtagcg gttgcggatg cgcacggtgc gcttgtgctc
      961 gaaatcgacg cccttcaggc tctcgatgaa cgtcgtgacg aaatgcctgc gggtctgctc
     1021 gccgtccgtg acgatgtcga tgcctgcggc ctcctgatcg gcaagcatga ggcgcatcgc
     1081 gtctcgcttg ccttcctcga gcagatcgcc ttcgaggagc cagggcgccc agagcttctt
     1141 cggctcggcg agccaggccg gtttaggaag actgccggcg atcgtggttt tcagttgcaa
     1201 aatagtctac gcgcgcgccg cgacgttcca cgaagcgaat ccgcagccga cgtagacctc
     1261 gggacagttg cagtagccga tcagctcgaa gccgcgcttg ccggccgcgc cgtgcgccgc
     1321 gacccagttg cggatctcgg cggcgccgtt gccgatcgtg ggcaggcgct cgttcaggaa
     1381 gtcgatcaag gggcgctcgt cgcctttttc gaaatggccg atgcattcgc ggtcgaacgg
     1441 ctcgtcgatc tcgcccatgg tgggctcccc gatcgaatgg gagaggccgc cggtggcgag
     1501 gatcgccacg cggatgtccc cgggataggt gcggaccgct tcggcgagca tggtccccca
     1561 ctgcaggcag cgccgcacgc tcgggtaggg cggctcgaga tcgttgaaga tgatcgggac
     1621 gatgggcggc aggggatcga ggccgccgcg ccacagcgga atgcagaagc cgtggtccag
     1681 cgtcaggtgg tgggacaccg agggctcgaa gtcgcgctcg agcgcggttt gcaggatgtg
     1741 attcgcgagc ttcggatgtc cggcgatctt cttgaaggga cagtccttca tccagggctc
     1801 ggcgggacct tcgtgctctt cgcccatgcc gacgcagatg ctcggcaggt tgttgatgaa
     1861 aaaattcacc cagtggtccg gggagatctc gaccagcacg tcgggcctgg cggccttgat
     1921 gcgcctgccg agctcggtca gcgagctcgt gagctgctct tgattcgcgg gcttgacgtc
     1981 tttccacccg cggacgatga gcggggcatg gctcgaggcg aacactccga ccagcctagc
     2041 catggctcga cctcgccgcg gcgtcggccc cggcggtcct cactttcttg aggaaatccg
     2101 cctcggacat cccggcgacc acgaaataaa acctgaggag ataagggttg acccgaggcg
     2161 cgaggaacgc gacgtcgtcg cgctcgaccg ctgtccgcac ctcgggcttc agaggatagc
     2221 ggtcgaggat cttcttccgg ttgacgcggt attcctcgcc gagcggagcg gagctctgca
     2281 gatcgaagaa gagcttgctc agccaatagt ctctcatggc cgctattttg ccatgagcgt
     2341 ggcgctactg tttcgtcccc cgatcgcggg tgagaggcac gaacctcacc ggcagggcgc
     2401 gcctggtcgt cgtgcggccg tcggattgtt tctccatgag caggaggtgc tgcacatccg
     2461 atggcgcgcc caccgggatc accattcgcc cgccgggctt gagctggtcg acgagcggct
     2521 gcgggaccgc cgcgggcgcc gccgtgacga tgatcgcgtc gaagggcgcg gcggcgggcc
     2581 agccgccgta gccgtcgccg acgcgcacct cgacgttgcg gtatcccagc tcgccgagaa
     2641 cttgcctggc gcggagtccg agggcctcga cgatctcgat cgtgtagacc ttcgccacca
     2701 gctcggcgag caccgccgcc tggtagcccg agccggttcc gacctcgagc acggtatcgg
     2761 tcggcttggg atcgagcagg tcggtcatca gcgcgacgat gtagggctgc gagatcgtct
     2821 ggccctcgcc gatcggcagc gggcgattgt cgtaggcgaa gatgtcctgc aggacgggga
     2881 cgaagcgatg tcgcggcact ttccccattg ccgccatcac cgcctcgccg aatctcggtc
     2941 gaccggtttc tgcaccggtt tccagcgcca gcgctgctat tcccgcgacc atctgcgcgc
     3001 gcgccgccgc gcgcgagtcc tgcgcctccg agggctgggc gtgcgtcacc gcgagcagga
     3061 aaagcgcgga gagcagggcg gccagcattt cgcgccagag ctcaggcgac caccaggctg
     3121 gtcaggcgct gcggatcggc aagcagatcg gcgctcggac cgctgtacgc gatgcggccg
     3181 cggttcagca cgagcgccct ctgcgtgatt ccgagcgcga ctttcgcgtg ctgttcgacg
     3241 agcacgatcg caagcgagtc ctcttggatc agccgttgca aggcctgcag cagcacctcg
     3301 acgatgatgg gcgcgaggcc ctcgagcggc tcgtccatga gcagcagcgt cgggttgccc
     3361 atcagggcgc gcccgatggc gagcatctgc tgctcgccgc cggagatctg gttgccccag
//
LOCUS       DASPZD010000032         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_103410, whole genome shotgun sequence.
ACCESSION   DASPZD010000032 DASPZD010000000
VERSION     DASPZD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103410"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VEM33_00625"
     CDS             complement(<1..1129)
                     /locus_tag="VEM33_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HYS50365.1"
                     /translation="MGKITGFLEFQRLEEAHQEPGERKKHYREFILRLTEEEAKVQGA
                     RCMDCGTPFCMTGCPVNNIIPDFNDLVYRQDWQRAIETLHSTNNFPEFTGRICPAPCE
                     EACVLRINDDPVGIKSIERAIIDKAFEEGWVRPQPPARKTGRKVAVVGSGPAGLACAQ
                     QLARAGHEVTVFEKNDRIGGLLRYGIPDFKMEKWLIDRRVDQMKAEGVVFRAGVFVGE
                     SRPGRGVTDWAAETVAPEKIVAEFHAVVLSGGAEQPRDLPIPGRELDGVRFAMEFLPL
                     QNKRVAGDKGVPDLWGTDKHVVVIGGGDTGSDCVGTSNRHGAKSVTQFELLAMPPDPE
                     LNPVWPYWPTRLRTSSSHEEGVNRDWSIATKEFVGENGKVKA"
     gene            complement(1130..>5129)
                     /gene="gltB"
                     /locus_tag="VEM33_00630"
     CDS             complement(1130..>5129)
                     /gene="gltB"
                     /locus_tag="VEM33_00630"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HYS50366.1"
                     /translation="DARVESALALVHQRFSTNTFPSWDLAHPFRMICHNGEINTLRGN
                     VNWIRARQGAISSPVLGRDLEKIWPLIYAGQSDSASFDNALELLVMGGYSVAHAMMMM
                     IPEAWENHTLMDPTRRAFYEYHAAMMEPWDGPASIAFTDGRQIGATLDRNGLRPSRYI
                     VTADDLVIMGSECGCLPVPEEKIVKKWRLQPGKMFLVDLEKGRIIDDEELKNTLAGAK
                     PYADWIERIRVKLDEVETEKTPPLKSAVRMLDRQQAFGYTQEDTKFIMAPMAMNGEEP
                     VGSMGNDSPLAVLSDKNKTLYHYFKQLFAQVTNPPIDPIREELVTSLVSFIGPKPNLL
                     GIDEMNPPLRLEVSQPVLDFFEMEKIRHIERYTGGKFRSFELDITYPLAWGKEAVEAR
                     LASLCAQAEDAVRAGYSIIVVSDRLVERERVAIPALLALSAIHQHLVAKGLRTSAGLV
                     VETGSAREVHHFALLGGYGAEAVHPYLALESLLDLAAKGELGPDFDGRKAIKNFVKAI
                     GKGLKKVMSKMGTSTYMSYTGAQIFEAVGLARSLVDKYFTGTTSSIEGIGVFEVAEEA
                     IRIHKAAFDDIDPVLQSMLDAGGEYAWRVRGEEHMWTPDAIAKLQHSARQNSPQAYKE
                     YAAIINDQSRRHMTFRGLFEFRFDRVKPVAIEEVEPAAEIVKRFSTGAMSHGSISVEA
                     HTTLAVAMNRIGGKSNTGEGGEDRKRYATVKAGETLRSRLGANRVARDIELREGDVLK
                     SKIKQVASGRFGVTAEYLASAEQLQIKIAQGAKPGEGGQLPGRKVSDYIAEIRYSTPG
                     VELISPPPHHDIYSIEDLAQLIHDLKNANPLASISVKLVSEVGVGTVAAGVSKAKSDH
                     VTISGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNRLRGRIAVQVDGQIKTGRD
                     VVIGAILGADEFGFATAPLVVSGCIMMRKCHLNTCPVGVATQDPVLRKKFSGKPEYVV
                     NYFFLVAEEARELMAQLGARSFSELIGRTELLDIKKGIEHWKAKGLDFSRIFKMPDMP
                     ADVARYNTEKQDHGLEKALDNRLIELAWPALDRGEKVSIDMPIRNGNRTVGTMLSHQI
                     ARRYGHEGLPEGTIHARFAGSAGQSFGAFLAKGVTLDLIGDTNDYCGKGLSGGRIIVS
                     PSPKFRGEPTENIITGNVVLYGAIAGDAYFRGVAGERFAVRNSGATAVVEGVGDHGCE
                     YMTGGTVAVLGATGRNFAAGMSGGIAYVLDLDGEFVKRCNTAMVDLEPVLAESEQQGK
                     VSRELWHKGQADEVILREMVERHAKLTNSRRAREILEKWADYRTRFVKIYPKEYRRAL
                     GELAAAKRKAAA"
BASE COUNT          837 a         1687 c         1601 g         1004 t
ORIGIN      
        1 gcgctttcac cttgccattc tcgccgacga attccttggt ggcgatcgac cagtcgcgat
       61 tgacgccctc ctcgtgcgag gaggaggtgc gcaatctcgt cggccaatag ggccaaaccg
      121 gattcaactc tggatccggc ggcatggcaa gcagttcgaa ctgcgtcacc gatttcgcgc
      181 catggcggtt ggaggtgccg acgcagtctg agccggtgtc gcctccgccg atcaccacga
      241 catgcttgtc cgtcccccaa agatcgggca cgcccttgtc cccggcgacg cgcttgttct
      301 gcagcggcag gaactccatc gcgaagcgca caccgtcgag ctcgcgcccg ggaatcggca
      361 ggtcgcgcgg ctgttcggcg ccgccggaaa gcaccacggc atggaactcg gcgacgattt
      421 tctccggtgc cactgtttcc gccgcccagt cggtgacgcc ccttccgggg cgggactcgc
      481 cgacgaagac gccggcacgg aacaccacgc cctccgcttt catttgatcg acccggcggt
      541 cgatcagcca cttctccatc ttgaaatcgg gtatcccgta gcgcaaaaga ccgccgatgc
      601 ggtcgttttt ttcgaacacg gtgacttcgt gcccggcgcg cgcgagctgc tgcgcgcagg
      661 cgagccccgc cggccccgat cccaccaccg cgactttcct gccagtcttt cgcgcgggcg
      721 gctgcggcct gacccagcct tcctcgaacg ccttgtcgat gatcgcgcgc tcgatcgact
      781 tgatcccgac cgggtcgtca ttgatgcgca gcacgcatgc ttcctcgcac ggagccggac
      841 aaatgcggcc ggtgaactcg ggaaaattgt tggtggaatg cagcgtctcg atggcgcgct
      901 gccaatcctg tctgtagacg agatcgttga aatccggaat gatgttgttg accgggcatc
      961 cggtcatgca gaacggggtg ccgcagtcca tgcaacgcgc gccctggacc ttcgcctctt
     1021 cttcggtgag gcggaggatg aactcccggt agtgcttttt ccgctccccg ggctcctggt
     1081 gcgcctcctc gagccgctgg aactcgagaa atcccgtgat tttccccatc tacgccgccg
     1141 ccttgcgttt cgccgcggcg agctcgccga gggcgcgcct gtattccttg gggtagatct
     1201 tcacgaaacg cgtgcggtag tccgcccatt tttcgaggat ctccctggcg cgccggctgt
     1261 tcgttagctt ggcgtgccgc tcgaccatct ctctcaggat cacctcgtct gcctgaccct
     1321 tgtgccagag ctcgcgcgac accttgccct gttgctcgga ctcggcaaga acaggctcca
     1381 ggtccaccat cgctgtgttg cagcgcttta cgaattcgcc gtcgaggtcg agcacatagg
     1441 cgatgccgcc ggacattccg gcagcgaagt tgcgtccggt ggcgcccagg acggcgacgg
     1501 tgcccccggt catgtattcg cagccgtgat ctccgacgcc ttcgacgacg gcggtggcgc
     1561 cggagttgcg cacggcgaaa cgctcgccgg ccacgccgcg gaaatacgcg tccccggcga
     1621 tcgcaccgta gagaacgacg ttgccggtga tgatattttc ggtcggttcg ccgcggaact
     1681 tgggcgaagg cgaaacgatg atgcgcccgc ccgagaggcc ctttccgcaa tagtcgttgg
     1741 tatcgccgat cagatcgagg gtcacgcctt tggcgaggaa cgccccgaag ctctggcccg
     1801 cggacccggc aaaccgggcg tggatcgtgc cctcgggcaa cccctcgtgc ccgtagcgtc
     1861 gcgcgatctg gtgcgagagc atcgtgccga ccgtacggtt gccgttccgg atcggcatgt
     1921 ctatcgaaac cttctcgccg cgatcgagcg cgggccacgc aagctcgatc agccggttgt
     1981 cgagggcctt ctcgagcccg tgatcctgtt tttcagtgtt gtagcgcgcg acgtccgccg
     2041 gcatgtcggg catcttgaaa atccgcgaga aatcgaggcc tttcgccttc cagtgctcga
     2101 tgcctttctt gatgtcgagc agttcggtcc tgccgatcag ctcgctgaag ctgcgtgcgc
     2161 caagctgggc catcagctcc cttgcctcct cggcgacgag gaagaagtag ttcacgacgt
     2221 actccggctt gccggaaaac ttcttgcgga gcacggggtc ctgagtcgcg acgcccaccg
     2281 ggcaggtgtt caagtggcat ttgcgcatca tgatgcagcc gctcacgacg agcggcgcgg
     2341 tcgcgaaccc gaattcgtcg gcgccgagga tcgcgccgat caccacatcg cggccggttt
     2401 tgatctgtcc atccacctgg accgcgatcc ggccacgcaa gcggttgagc accagggtct
     2461 gctgcgtctc cgcgaggccc agctcccagg gcgtgcccgc gtgcttgatc gacgaccaag
     2521 gcgaggcgcc cgtgccgccg tcgtgcccgg aaatcgtgac gtggtcggac ttcgccttgg
     2581 acacgcctgc agccacggtg ccgacgccca cttccgagac gagcttcacc gagatggatg
     2641 ctaaagggtt cgcgttcttg agatcgtgga tcagctgggc gaggtcctcg atcgagtaga
     2701 tgtcgtggtg cggcggaggc gaaatcagct ccactccggg agtcgagtag cggatctcgg
     2761 cgatgtaatc ggacaccttg cgcccgggca gctgcccgcc ctcgccgggc tttgcgccct
     2821 gggcgatctt gatctggagc tgctcggcgc ttgcgagata ttcggcggtc acaccgaagc
     2881 gccccgaggc gacctgcttg atcttggact tgagcacgtc gccttcgcgc aattcgatgt
     2941 cgcgcgcgac gcggttcgcg ccgagacgcg agcgcagcgt ctcgccggcc ttcactgtcg
     3001 cgtagcgctt gcggtcctcg ccgccctcgc cggtgttcga ctttccgccg atgcggttca
     3061 tcgccacggc gagcgtggtg tgcgcttcga ccgagatcga accgtgcgac atggcgcctg
     3121 tcgaaaaacg cttcactatc tcggctgcgg gttcgacctc ttcgatcgca acgggcttca
     3181 cgcgatcgaa gcggaattcg aacaggccgc ggaaagtcat gtggcgccgg gactggtcgt
     3241 tgatgatcgc ggcgtattcc ttgtacgcct ggggcgaatt ctgccgcgcg gagtgctgca
     3301 gtttcgcgat cgcgtcgggc gtccacatat gctcctcgcc gcgcacgcgc caagcgtact
     3361 cgcccccggc atccagcatc gactgcagca ccgggtcgat gtcatcgaac gcggccttgt
     3421 gtatgcggat cgcttcttcg gcgacttcga acacgcctat gccttcgatc gacgaggtgg
     3481 tcccggtgaa atacttgtcc acgagcgatc ttgcgagacc taccgcctcg aagatctgcg
     3541 cgccggtgta ggacatgtag gtcgaggtcc ccatcttgga catcaccttc ttcaggccct
     3601 tgccgatcgc cttcacgaaa ttcttgatcg cctttcttcc gtcgaagtca gggccgagct
     3661 cgcccttcgc agcgagatcg agcagcgact cgagagcgag atacgggtgc accgcctcgg
     3721 cgccgtaacc tccgagcaag gcaaagtgat gcacctcgcg cgccgagccc gtttccacca
     3781 ccagaccagc gctcgtgcgc agacctttcg ccaccaggtg ctgatggatc gccgaaagcg
     3841 ccaggagcgc cggtatcgcc acgcgttccc gctccacgag ccggtcggaa accacgatga
     3901 tcgagtaacc cgcgcgcacc gcatcctcgg cctgcgcgca aagcgacgcg aggcgcgcct
     3961 cgaccgcctc cttgccccac gcgaggggat aggtgatgtc cagctcgaac gagcggaact
     4021 tgccgccggt gtagcgctcg atgtgccgga tcttctccat ttcaaagaag tcgagcaccg
     4081 gctgagacac ctccagccgg agaggcgggt tcatctcgtc gatgccgagc aggttcggct
     4141 tcggtccgat gaaagacacc agggacgtca cgagctcctc gcggatcggg tcgatcgggg
     4201 ggttcgtgac ctgcgcgaac agctgcttga agtagtggta cagcgtcttg ttcttgtccg
     4261 acagcaccgc aagcggtgag tcgttgccca tcgagccgac gggttcctcg ccgttcatcg
     4321 ccatcggggc catgatgaac ttggtgtcct cctgcgtgta gccgaaggcc tgctggcgat
     4381 cgagcatccg caccgcgctc ttgagcggcg gggtcttttc ggtctcgacc tcgtcgagct
     4441 tgacccggat gcgctcgatc caatccgcgt agggtttcgc gcccgcgagc gtgttcttga
     4501 gctcctcgtc gtcgatgatc cggcccttct ccagatcgac gaggaacatc tttccgggct
     4561 gcaggcgcca cttcttgacg atcttctcct cgggcacggg caggcagccg cattccgagc
     4621 ccatgatcac gaggtcgtcc gcagtgacga tgtagcgcga gggccgcagg ccgttcctgt
     4681 cgagggtcgc ccctatttgg cgcccgtccg taaacgcgat cgatgcgggc ccgtcccagg
     4741 gctccatcat cgcggcgtga tattcataga atgcgcgccg cgtcgggtcc atcagcgtgt
     4801 ggttctccca ggcctcgggg atcatcatca tcatcgcgtg cgcgaccgaa taccctccca
     4861 ttaccaggag ctcgagcgcg ttgtcgaacg aagcggaatc ggattgcccg gcgtagatca
     4921 gcggccagat tttctcgagg tcgcggccga gaaccgggct cgagatcgcg ccctggcgcg
     4981 cgcggatcca attgacgttg ccccgcaagg tgttgatctc gccgttgtgg cagatcatgc
     5041 ggaacgggtg cgccaggtcc caggatggga aggtattcgt ggaaaatcgc tggtggacca
     5101 aggcgagcgc ggactcgacg cgcgcatcc
//
LOCUS       DASPZD010000033         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_222072, whole genome shotgun sequence.
ACCESSION   DASPZD010000033 DASPZD010000000
VERSION     DASPZD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222072"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..991)
                     /locus_tag="VEM33_00635"
     CDS             complement(119..991)
                     /locus_tag="VEM33_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS50367.1"
                     /translation="MTPARRIAAEFAASRIALASAALLALVLLAALLAPWISPQNPYD
                     LARLDLLDSRLPPGARSAAGAIHWLGTDDQGRDMLSAILYGLRTSIAVGFSSTILGLV
                     IGVSLGLAAAWFGGWLDALLMRIADLQLSFPSILIALVLLAVLGQGLDKIVIALVAVQ
                     WAYYARTARAVALVERRKEYIDAAVALALSNARIISRHLLPNCLPPLLVLATVQVAAA
                     ITLEATLSFLGLGLPITQPSLGLLISNGFNYLLSGHYWISAFPGIALLLAVVAINLVA
                     DRLGEVLDPHGRHR"
     gene            complement(988..1962)
                     /locus_tag="VEM33_00640"
     CDS             complement(988..1962)
                     /locus_tag="VEM33_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS50368.1"
                     /translation="MLALCVRRLAQSVAVLFVMSLLVFAGVYAVGNPVDILVNPQSDQ
                     NEIARATAALGLDRPLLEQYFVFVGRAATGDLGRSFAFNVPAIGLILERMPATLELAT
                     AAMLIAVVFGIPLGLWAGLKPRSAAGRIIMAGSVLGFSLPTFWVGLMLILVFSVWLGW
                     LPTSGRGPTVNLLGIPLSFLTLEGLRHLVLPAANLALFNLSLVIRLTRSGTREAMRQD
                     YVRFARAKGLTERRVVGVHVLRNVLIPVVTVAGVEFGSVIAFAVVTESIFAWPGMGKL
                     LIDSINVLDRPVIVGYLCIVVTLFVAINLFVDLAYSWLDPRVRSGGAR"
BASE COUNT          397 a          638 c          726 g          262 t
ORIGIN      
        1 ggcttgggcc cctgcgcgag gcccgcgccg gttgcggcga aaagggccgc agcggccgcg
       61 attttcttga gtgcgttcat ggtttctcct ccgaatattt caagcgacag tataagactc
      121 accggtgccg cccgtgcgga tcgaggacct cgcccaagcg gtccgctacc aggttgatgg
      181 cgacgacggc gaggagcagg gcgattccgg ggaaggcgct gatccagtag tggcccgaga
      241 gcagatagtt gaagccgttc gagatcagca ggccgagcga aggctgggtg atcggcaggc
      301 cgagtcccag gaaggaaagc gtggcttcga gcgtgatcgc cgcggcgacc tggacggtcg
      361 cgagcacgag tagcggcggc aggcagttcg gaagcaagtg acgggaaatg atgcgcgcgt
      421 tcgacagcgc gagcgcgact gcggcgtcga tgtattcctt cctgcgctcg acgagcgcaa
      481 ccgcgcgcgc cgtgcgggcg tagtaagccc attggaccgc gacgagcgcg atcacgatct
      541 tgtccaggcc ttgcccgagc acggcgagca ggacgagcgc gataaggatc gaagggaacg
      601 agagctgaag atcggcgatg cgcatgagaa gcgcatcgag ccagcctccg aaccaagccg
      661 ccgcgaggcc caggcttacg ccgatgacca ggccgagaat cgtgctcgag aaaccgacgg
      721 cgatgctggt gcgaaggccg tagaggatgg cggacagcat gtcgcggccc tggtcgtcgg
      781 tgcccagcca atggatcgcg cccgctgccg agcgcgcccc cggcggcagg cgcgagtcga
      841 gcaggtcgag ccgcgccaga tcgtaggggt tctgcggcga gatccacggc gcaaggagcg
      901 cggcgagcag caccagcgcg agaagcgcgg cactcgcgag cgcgatgcgg ctcgccgcga
      961 actcggcggc gattcgccgc gcgggcgtca ccttgctcct ccggagcgca cccgcggatc
     1021 gagccacgag taggcgaggt cgacgaagag attgatcgcg acgaacagcg tgacgacgat
     1081 gcacagatag ccgacgatca ccggccggtc gagcacgttg atcgaatcga tcaggagctt
     1141 tcccatgccg ggccaagcga atatcgactc ggtgacgacg gcgaacgcga tcaccgaacc
     1201 gaattccaca ccggccaccg tgacgaccgg aatgagcacg ttcctgagca cgtggacccc
     1261 gacgacgcgc ctctcggtca ggccttttgc gcgcgcgaag cgcacgtagt cctgccgcat
     1321 cgcttcgcgc gttcccgagc gggtcagccg gatgacgagc gacaggttga atagcgccag
     1381 gtttgcggcg ggcaggacga ggtggcgcag gccctcgagc gtgaggaagc tcagcgggat
     1441 cccgaggaga ttcactgtcg gcccgcggcc cgaggtcggc agccagccga gccagaccga
     1501 gaacacgagg atgagcataa gcccgaccca gaaagtcggg agggagaacc ccaggaccga
     1561 gcccgccatg atgatcctgc cggcggccga ccggggcttc aggcccgccc agagcccgag
     1621 cgggattccg aaaacaaccg cgatcagcat cgcggcggtc gcgagctcga gcgtcgcggg
     1681 catgcgctcg aggatcaggc cgatcgccgg cacgttgaag gcgaatgatc tcccgaggtc
     1741 tccggttgcg gcgcgcccga cgaagacgaa atactgctcg aggagcggcc ggtcgagccc
     1801 caacgcggcg gtggcgcgcg cgatctcgtt ctggtcggac tgcgggttga cgaggatgtc
     1861 caccgggttc ccgaccgcgt atacgcccgc gaacacgagc agcgacatga cgaacaggac
     1921 cgcgacgctc tgcgcgaggc ggcgaacgca aagggcgagc atcagcgggc ggtcccggcg
     1981 atgcgcagca gctcttctcc cagcgccagc ccggcgtcgg gcc
//
LOCUS       DASPZD010000034         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_221862, whole genome shotgun sequence.
ACCESSION   DASPZD010000034 DASPZD010000000
VERSION     DASPZD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221862"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VEM33_00645"
     CDS             complement(<1..139)
                     /locus_tag="VEM33_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HYS50369.1"
                     /translation="MGPARQYRYYEFVMAAFVTVLICSNLIGPAKIAQFDFPLIGIVS
                     FG"
     gene            complement(139..1152)
                     /gene="mltB"
                     /locus_tag="VEM33_00650"
     CDS             complement(139..1152)
                     /gene="mltB"
                     /locus_tag="VEM33_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HYS50370.1"
                     /translation="MRRTLLAALLVLHAALASAQQPGYGQRKEVKEFITEMVKKHKFS
                     RKDLNRVFAKAQYQPAIVKAMDQPPESALASWQAYRSIFIKPERIEAGAQFWNRNREA
                     LKRAAGEYGVPEDIIVGIIGVETTFGRNIGTFRVIDALSTLAFDYPKRSAYFRGELEH
                     YLVFSREQKIDPLRVKGSYAGAIGIPQFMPGSYRRFAVDYDGDGQINLATSPADAIGS
                     IGNFLKSHGWVRGQPVAFAAETSGDGWRKLAGAGIEPATRFADLAGHGIKLPVALPDD
                     TLCALVELESPGEPLDVRVTLQNFFVLTRYNRSNLYAAAVLDLAAEIVRARAAAAPAK
                     SNS"
     gene            complement(1149..>2866)
                     /locus_tag="VEM33_00655"
     CDS             complement(1149..>2866)
                     /locus_tag="VEM33_00655"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HYS50371.1"
                     /translation="RTIMGRDPGVTLLVLLLFLKLLETRARRDIFVVAFLVYFVALAN
                     FFYSQSIPIAGLMLLTVLVTTTALVGFSAPLRPFVDDLKTAGRMLAQAGPVMLLLFFL
                     FPRVQGPLWGMPQDAYSGVTGLSETMSPGSIRDLSLSDAIAFRVKFETEAPPRALLYW
                     RGPVLSDFDGLAWRVGLPQLRRNMSIEATGSPVDYEVTLEPHNRNWMFALEMPARLPP
                     SARLTSEYLVISLTPIRSRIRYEMRSFPQFQAASGANPEDLALALRLPRGVNPRSLAL
                     AREWRESLADNSAIVRRAIEFFRGSRFEYTLQPPALGQNSVDEFLFDTKQGFCEHFAS
                     SFVFLMRAAGVPARVVTGYQGGDTNPVDGYMVVRQADAHAWAEVWLAGIGWTRVDPTA
                     AAVPVRVELGITAAAPAGATLPLLMRTNLNWLKGLRNNWEALTNQWNQWVLGYNPDRQ
                     REMLSWLGVPQPSWQTMTVMLFWSVAGVLLLTALWLLRGMRREDTVQRAWLRFCDKLA
                     REGLARARAEGPLDYADRVAQALPARENAVRAIASLYVDQRYGPGARTESVARLKELV
                     RDFTP"
BASE COUNT          512 a          932 c          964 g          458 t
ORIGIN      
        1 ctccgaacga gacgatcccg atcaacggga agtcgaactg cgcaatcttg gccgggccga
       61 tcaggttcga gcagatcagc acggtgacga acgccgccat cacgaattcg tagtagcggt
      121 attggcgcgc cggccccatc acgaattgct cttggccggt gcggcggccg ctctcgctct
      181 cacgatctcg gccgccagat cgagcacggc ggcggcgtag agattggagc ggttgtagcg
      241 cgtgagaacg aagaaattct gcagcgtcac gcggacgtca aggggctccc ccggcgactc
      301 gagctcgacc agcgcgcaca gggtatcgtc cggcagggcg accggaagct tgataccgtg
      361 tcccgccagg tccgcgaagc gagtcgccgg ctcgatgccc gcgccggcga gcttgcgcca
      421 tccgtctccc gacgtctctg ccgcgaaggc gaccggctgc cctcggaccc agccgtgcga
      481 cttcaggaaa ttgccgatgc tgcctatcgc gtcggcgggg ctcgtcgcga ggttgatctg
      541 cccgtcgccg tcgtagtcaa ccgcgaagcg ccggtacgac ccgggcatga actgggggat
      601 gccgatcgcc cccgcgtacg agcccttcac gcgcagcgga tcgattttct gctcccgcga
      661 gaacacgagg tagtgttcca gctcgccgcg gaagtaggcg ctgcgcttgg ggtagtcgaa
      721 ggccagggtg gatagggcgt cgatgacgcg gaaggtaccg atgttgcggc cgaaggtggt
      781 ttcgacgccg atgatgccga ctatgatgtc ctcgggcact ccgtattcgc ccgcggcgcg
      841 cttgagcgcc tcgcggttgc ggttccaaaa ctgcgcgccg gcctcgatcc tctcgggctt
      901 gatgaagatc gagcggtacg cctgccagga agcgagcgcc gattcggggg gctggtccat
      961 cgccttcacg atcgccggct gatattgcgc cttcgcgaac acccggttca ggtccttgcg
     1021 gctgaatttg tgcttcttca ccatctccgt gatgaattct ttcacttcct tgcgctggcc
     1081 gtagccgggc tgctgtgccg acgccagggc ggcgtggagc acgagcaagg ccgcgaggag
     1141 tgtccttctc aaggcgtgaa atcccgaacg agttctttca ggcgcgcgac cgattcggtc
     1201 ctagcgcccg ggccgtagcg ctgatcgacg tagagtgagg cgatggcgcg cacggcgttc
     1261 tcgcgcgccg ggagcgcctg ggcaacgcgg tccgcgtagt cgagcgggcc ctcggcgcgc
     1321 gcgcgcgcaa ggccctcgcg cgcgagcttg tcgcagaagc gcagccaggc gcgctgcacc
     1381 gtatcctcgc gccgcatgcc gcgaagcagc caaagcgcag tcagcagcag caccccggcc
     1441 acgctccaga acagcatcac ggtcatcgtt tgccaactcg gctgcggcac gcccagccac
     1501 gaaagcattt cgcgctggcg atcggggttg taaccgagca cccactggtt ccactggttc
     1561 gtgagagcct cccagttgtt gcgcaggccc ttcagccaat tgagattggt gcgcatgagc
     1621 agcggcagcg tcgctcctgc cggcgccgcg gccgtgatgc cgagctcgac tcggaccggg
     1681 accgctgcgg cggtgggatc gacgcgagtc cagccgatcc cggcaagcca cacttcagcc
     1741 caggcgtggg cgtcggcctg gcgcacgacc atgtagccgt ccactggatt ggtgtctcct
     1801 ccctggtaac cggttaccac gcgggcgggc acgccggcag cgcgcatcag aaacacgaaa
     1861 ctcgaagcga agtgctcgca gaatccctgc ttcgtgtcga acagaaactc gtccacggag
     1921 ttctggccga gcgccggcgg ctgcagcgtg tactcgaagc ggctgccgcg gaagaactcg
     1981 atagcgcggc gcacgatcgc cgaattgtcg gcgagcgact cgcgccattc gcgcgcgagc
     2041 gcaagcgaac gcggattgac gccacgggga agcctcagcg ccagcgcgag gtcctcggga
     2101 ttggctccgc tcgccgcctg aaattgggga aaggagcgca tttcgtaccg gatccggctg
     2161 cggatcggcg tcagggaaat gacgaggtat tcggaggtga gacgcgcgct cggcgggagg
     2221 cgcgccggca tttccagagc gaacatccag ttgcggttgt gcggctcgag ggtcacctcg
     2281 taatcgaccg gcgagccggt ggcctcgatg ctcatgttcc ggcgcagctg cggcaggcct
     2341 acgcgccagg cgaggccgtc gaaatccgac agcaccgggc cgcgccagta gagcagggcg
     2401 cgcggcggcg cttccgtttc gaatttcacc ctgaacgcga tcgcgtccga cagggacagg
     2461 tctctgatcg agccgggcga catggtctcc gacaggccgg tcacgccgct gtaggcgtcc
     2521 tgcggcattc cccacagggg cccctgcact cgagggaaca ggaaaaacag cagcagcatc
     2581 accggtcccg cctgggccag catccgcccg gccgttttca gatcgtcgac gaagggccgc
     2641 agcggcgcgc tgaacccgac gagcgccgtc gtggtgacca acacggtcag cagcatcagc
     2701 cccgcgatcg gaatggactg ggagtagaaa aagttggcca gagcgacgaa gtacacgaga
     2761 aacgccacga caaagatgtc gcgccgggcc cgagtctcca gcagtttgag aaacaagagc
     2821 agcacgagga gagtcacccc cggatcccgg cccatgatcg tgcgat
//
LOCUS       DASPZD010000035         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1549456, whole genome shotgun sequence.
ACCESSION   DASPZD010000035 DASPZD010000000
VERSION     DASPZD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549456"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1243
                     /locus_tag="VEM33_00660"
     CDS             5..1243
                     /locus_tag="VEM33_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HYS50372.1"
                     /translation="MLGSGPVGEATRAHHAESAVSLQAFFASCPRGLESLLAEELGPL
                     GAKKPEPVSGGVAFEGDMRACYRANLESRLATRVLMRLARSPYRGEKDVYEAALAVKW
                     SEWFSETQSIRVDVNAIRSPLKSLEFATLRIKDAVCDRFRADRGRRPDVDTRSPEVRI
                     QAFLDKAEVTLYLDTSGEPLNKRGYRRDAGEAPLKENLAAGIVRLTGWNQGEPLLDPM
                     CGSATLVVEAAMMALSIPPGHARAFGFERLAGFDLRRWNEVRDAALARRKPKQRLPIF
                     ARDRYGEELKKARLNLEAAGLADCVDLKQADVLDTGAPARAGVLLANPPYGTRIGEEA
                     ALAEFYPKLGDTLKKYYAGWRCYLFSGDARLPKLIRLTATKRTPLYNGPLECRLYEYR
                     VVAGSNRKKSTPSRASGPGA"
     gene            complement(1259..1696)
                     /locus_tag="VEM33_00665"
     CDS             complement(1259..1696)
                     /locus_tag="VEM33_00665"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HYS50373.1"
                     /translation="MNPENVIDVTRLIQLAVAPVFLLTAVGTIIGVLSIRLGRVVDRS
                     RTLEDRLRQLQPAGQKAARQELDVLSRRVRLVYGSIVLSVVCALFVGLLIAVAFVDAF
                     VDIDLSKFIGLLFIAAMLAFILALLVFLREIFLAVSSARGQMR"
     gene            complement(1728..2051)
                     /locus_tag="VEM33_00670"
     CDS             complement(1728..2051)
                     /locus_tag="VEM33_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50374.1"
                     /translation="MAHTFPELKSKPLAELREIAAGIEHEAVKGYTQLNKDHLLAALC
                     KALNIDMHVHHEVKGVDKTAIKTKIGEWQKKRDEALAAKDRGKLKVALNHIHHFKHQL
                     RKAMV"
     gene            complement(2112..2626)
                     /locus_tag="VEM33_00675"
                     /pseudo
     CDS             complement(2112..2626)
                     /locus_tag="VEM33_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          502 a          964 c          900 g          487 t
ORIGIN      
        1 ggctgtgcta ggctccggtc ccgtcggcga agccactcga gcccatcatg ctgaatccgc
       61 cgtgagcctg caagccttct tcgcctcctg tccgaggggc ctcgaatcgc ttctcgccga
      121 agagctcggc ccgctcgggg cgaagaaacc cgagcccgtt tcgggcgggg tcgcattcga
      181 gggcgacatg cgggcctgtt accgcgccaa tctcgagtcc cggctcgcca cgcgcgtcct
      241 gatgcggctt gcccgctcgc cgtaccgcgg agagaaagac gtatacgaag cggcactcgc
      301 ggtgaagtgg tccgaatggt tcagcgagac gcaaagcatc cgcgtcgacg tcaacgcgat
      361 acgctctccg ctgaagagcc tcgaattcgc gactttgcga atcaaggacg ctgtgtgcga
      421 ccggtttcgc gcggaccgcg ggcggcggcc cgacgtggac acgcgctccc ccgaggtgcg
      481 catccaggct ttcctcgaca aggcggaagt caccctttac ctggacacct ccggcgaacc
      541 gctcaacaag cgcggctacc gcagggacgc gggcgaggcc ccgctgaagg agaaccttgc
      601 cgccggcatc gtccggctca cgggctggaa ccagggcgag cccctcctcg accccatgtg
      661 cggcagcgcg acgctcgtcg tcgaggcggc gatgatggcg ctctcgatcc cgccgggaca
      721 cgcgcgcgca ttcggcttcg agcgcctcgc cggattcgat ctcaggcggt ggaacgaggt
      781 gcgcgacgcg gcgctcgccc gccgcaagcc caagcagcgt ctcccgatct tcgcccgcga
      841 ccgctacggc gaggagctca agaaagcccg cctcaacctc gaggcggcgg gccttgccga
      901 ctgcgtcgac ctcaagcagg ccgacgttct cgataccggc gccccggcgc gcgcgggagt
      961 gctcctcgcc aacccgccct acggcacccg gatcggcgag gaggcggcgc tcgcggagtt
     1021 ctacccgaag ctgggcgata cgctgaagaa atactacgcg ggctggcgct gctacctctt
     1081 ctcgggggat gcgcgacttc cgaagctgat cagattgacc gcgaccaaac gcacgccgct
     1141 ttacaacggc cccctggaat gccggctcta cgagtatcgg gtcgtcgccg ggtccaaccg
     1201 caagaagtcc actccatcgc gcgcgagcgg accgggcgct tagcgcctta gcgcattcct
     1261 accgcatctg gccgcgcgcg ctcgataccg ccaggaagat ctcgcgcaga aacaccaaca
     1321 gtgcgaggat gaaggcgagc atcgcggcga tgaagagcag cccgatgaac ttcgacagat
     1381 cgatgtcgac gaacgcgtcg acgaaagcga ccgcaatcag cagtccgacg aaaagcgcgc
     1441 aaaccactga gagcacgatc gagccgtaca ccaggcgcac gcggcggctg aggacatcga
     1501 gttcctggcg ggccgctttc tggcccgcgg gctggagctg gcgcagccgg tcctcgagcg
     1561 tacggctgcg gtcgaccacg cggccgaggc gaatggagag cacgccgatg atcgtcccca
     1621 cggcggtgag caggaagacc ggcgcgaccg cgagctggat aagccgcgta acgtcgatga
     1681 cgttctcggg attcaccacc ggagccgttc ctgcccgccg ccgcgcttca caccatcgct
     1741 ttccgcaact ggtgcttgaa atggtggatg tggttgagcg cgaccttcag cttgcctcgg
     1801 tcctttgcgg cgagcgcttc gtcgcgcttt ttctgccact cgccgatctt ggttttaatc
     1861 gcggtcttgt cgacgccctt gacctcgtgg tgcacgtgca tgtcgatgtt gagcgccttg
     1921 cagagcgcag cgagcaaatg gtccttgttc aactgcgtgt agcctttcac cgcctcgtgc
     1981 tcgatccccg ccgcgatctc gcgcagctcg gcgagaggct tgctcttcag ctcaggaaag
     2041 gtgtgggcca tgcgtcctcc ggggtgattg tcccagatga tactgtagag cgtccgcggc
     2101 agctaccgcg gctacctgtt cgcgttccct acgcgtcttc cgacgatgat gagatcgcgc
     2161 tcgaccccgt ccaacaaagc gaccttcggc atctcccccc accgctggaa tccgaatcgc
     2221 cggaacaacg caaggctcgg ttcgttgtgg ccgaatatga accccaacag cgtgtcgacc
     2281 ccgaacgtcg gcgcgtgagc cggcgcttgg gtcagcagat aggagccgag cccgcgcttt
     2341 cggaacgcct cgtgaacgta gacgctcagc tccgcggtct tggcgtatgc cgggcgcccg
     2401 tagaacgctg aaaagctgag ccaggccgca atgcgacccg cgtcctcgac acccaaagag
     2461 gcctgcgatc gggcgtgtgt tcctcgaacc agtgcacccg gctctcgacc gaaacgggct
     2521 cggtatcggc cgtcgcgatc cgcgacggaa tcgtggagtt gtagatctcc acgatctgcg
     2581 ggaggtcgcc tcgaattgcg gtcctgtgga ctgatgggtc aggcatcgac ccctctcaag
     2641 gcgggacggt ccatggaccc agggcgccta gtgcttcctc ttcgctcatg gcctttgcgc
     2701 gttctctcac ttcaaccgga acccattttg gaaacgggac ctccctgttc acatggcgtc
     2761 ccgaagcggt gtgctcttgc cgcaactgcg cgacctgatt cttcgggaat cgctccgcca
     2821 cgcggacaag ggcgggccaa agtcggcgat gaa
//
LOCUS       DASPZD010000036         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_581857, whole genome shotgun sequence.
ACCESSION   DASPZD010000036 DASPZD010000000
VERSION     DASPZD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581857"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VEM33_00680"
     CDS             <1..631
                     /locus_tag="VEM33_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50375.1"
                     /translation="SFEHVSLLVTSPQALVGATNFAPNTVQELLTLAKANPGKVTYAT
                     VGAGSSNHLNALLLSNRTGIEMTHVPYKGGAPMMIDLVGGQVNVMFVSAPQAIPQVKA
                     GRIKALAIGSARRIPQLPDTPTVAETLPGFAAQSWVGMLVPAGTPKEIVARLYAEATR
                     ALADSEVRGKLEAQGFDVVGSAPGEFLAFARAESEKWAKVIRDYNIRVE"
     gene            646..1605
                     /locus_tag="VEM33_00685"
     CDS             646..1605
                     /locus_tag="VEM33_00685"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYS50376.1"
                     /translation="MARDTKWIPQGVIPAVLLPFHEDLSIDEPSFRAHLRDVASVDGL
                     SAVTINAHSTEVASCSFDEQERVLAIAKDEIGDRLPIVHGVYAEGSLEAARIDRMAAE
                     GGAAALLVFPPGPFTMGQRPEMAVEHFRRVAGASELPIIAFQYPSAGGQGYPLATLLK
                     IIEAVPQVTAIKDWCASPQLHERQIRTLQSLKPPVNVLTTHSSWLISSLVMGCNGLLS
                     GSGSVIADLQARLFRAVRANDLATARQINERIWPTAEAFYSEPWVDMHNRMKEALVMM
                     GKLPRAVMRPPLVKIPQAEIERIRKALISAGLLDRAGRSLRAA"
     gene            1670..>2010
                     /locus_tag="VEM33_00690"
     CDS             1670..>2010
                     /locus_tag="VEM33_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYS50377.1"
                     /translation="MKIECVLECANHLGEGPVWDVEESRLYWVDGTGRRVGKPSIWRL
                     DPKSGKTENWALKRDIGALALREKGGAVLALDDGFHFFDFQSGKLETVSLVDTEHSRT
                     RLNDGKCDRRGR"
BASE COUNT          346 a          660 c          682 g          322 t
ORIGIN      
        1 gtccttcgag cacgtgagcc tgctcgtcac ctcgcctcag gcgttggtcg gcgcgaccaa
       61 cttcgcgccg aacaccgtgc aggagctcct gacgctcgcg aaagccaatc cgggcaaggt
      121 gacctacgcc acggtgggcg cgggaagctc gaaccacctg aacgccttgc tgctttcgaa
      181 ccgcaccggg atcgagatga cccacgtgcc ttacaagggc ggcgcgccca tgatgatcga
      241 tctggtcggc ggccaggtga acgtgatgtt cgtgtccgcg ccgcaggcga ttccgcaggt
      301 gaaggccgga cgcatcaagg cgctcgcgat cgggagcgcg cgccgcatcc cccagctccc
      361 ggacacgccg accgtcgccg agacgctccc cgggttcgcg gcgcaatcct gggtggggat
      421 gctcgtgccg gccggaacgc cgaaggagat cgtagcgcgg ctctacgccg aagcgacgag
      481 ggcgctcgcc gattccgaag tgcgcggcaa gctcgaggcc caggggttcg acgtcgtcgg
      541 cagcgcgccc ggggagttcc tcgcctttgc gcgcgccgaa tcggaaaagt gggcgaaggt
      601 gatccgcgat tacaatatcc gggtcgagta actcaaggac tcccgatggc gcgcgacacg
      661 aagtggattc cccagggtgt catccccgcg gtgctgcttc cgttccacga ggacttgtcg
      721 atcgacgagc cgtccttccg cgcgcacttg cgcgacgtcg cctcggtgga tggactgtcc
      781 gcggtcacca tcaacgcgca ttcgaccgaa gtcgcctcgt gctcgttcga cgagcaggag
      841 agggtgctcg cgatcgcgaa ggacgaaatc ggagatcggc tgccgatcgt gcacggggtg
      901 tacgccgaag gcagcctcga agcggcgcgc atcgacagga tggcggccga aggcggcgcg
      961 gcggcgctcc tcgttttccc gcccgggccc ttcacgatgg ggcagcgccc ggagatggcg
     1021 gtcgagcact tccggcgcgt cgccggtgcg tctgagcttc cgatcatcgc ctttcaatac
     1081 ccgtcagcag gcggccaggg ctatccgctc gcgaccttgt tgaagatcat cgaggcggtg
     1141 ccgcaggtga cggcgatcaa ggactggtgc gcgagtcccc agctgcacga gcgccagatc
     1201 cggacgctgc agtcgctcaa gccccctgtg aacgtgctca ccacgcacag ctcgtggctg
     1261 ataagctcgc tggtgatggg ttgcaacggg ttgctctccg ggagcggctc ggtgatcgcg
     1321 gatctgcagg ccaggctttt ccgggcggtg cgtgcgaacg acctcgccac cgcgaggcag
     1381 atcaacgagc gcatctggcc cacggccgag gcgttctact ccgagccctg ggtcgacatg
     1441 cacaaccgca tgaaggaagc gctggtgatg atgggcaagc ttccgcgcgc ggtgatgcgc
     1501 ccgccgctcg tcaagattcc gcaagccgag atcgagcgca tccgcaaagc gcttatctcg
     1561 gccggcctgc tcgaccgggc cggccgctcg ctccgcgccg catgaaaaac cggggacaga
     1621 ccacggtttc ctcgtcgtca gcccgaaaag gtggtctgtc cccggtttca tgaagatcga
     1681 atgcgtcctc gagtgcgcca accatctcgg cgagggcccg gtctgggacg tcgaggagag
     1741 ccggctctat tgggttgacg ggaccggccg gcgcgtcggc aagccttcga tctggaggct
     1801 cgatccgaag agcggcaaga ccgagaactg ggcgctcaag cgcgacatcg gggcgctcgc
     1861 gctgcgcgag aaaggcggcg cggtgctcgc gctcgacgac ggcttccact tcttcgattt
     1921 ccagagcggc aagctcgaaa ccgtatcgct cgtcgatacc gaacactcgc gcacgcgcct
     1981 gaacgacggc aagtgcgacc ggcgcggtcg
//
LOCUS       DASPZD010000037         4958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1062741, whole genome shotgun sequence.
ACCESSION   DASPZD010000037 DASPZD010000000
VERSION     DASPZD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062741"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..929
                     /gene="hemF"
                     /locus_tag="VEM33_00695"
     CDS             27..929
                     /gene="hemF"
                     /locus_tag="VEM33_00695"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HYS50378.1"
                     /translation="MNPATVRTYFLDLQNRLVAELERIEGRSFRSDQWQRSEGGGGES
                     RIIEGGDVFERGGVNFSRVHGERLPPSASAARPELAGRAFEATGVSLVLHPRNPYVPT
                     VHMNVRFLVATKPQSEPTWWFGGGMDLTPYYGFEEDARHFHATCRRALEAFGSDCYPR
                     FKRWCDEYFYLKHRGEPRGIGGIFFDDLNESGFDSCFELARRVGDHFLPAYLPIIERR
                     KDLPYGERERDFQAYRRGRYVEFNLVYDRGTLFGLQSAGRAESILMSLPPLVKWRYDW
                     KPDPSSAEGKLYRDFLRARDWLGE"
     gene            948..1940
                     /locus_tag="VEM33_00700"
     CDS             948..1940
                     /locus_tag="VEM33_00700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYS50379.1"
                     /translation="MDTRKLGTNGPAVSALGLGCMGMTGIYGEPNEPESIATIHRAID
                     LGVNLIVTSDAYGAGNNEELVGRAIKGRRNRVLLATKFGNVGLSGFKLPEGLSAGHPA
                     YVPQACEASLKRLGVEVIDIYGLHRVDPKVPIEDTVGAMKMLVDQGKVRHLALSEAGA
                     ATLRRAHKVHPLVALETEYSLWSRDVEKDILPACRELGIAFMAYSPLGRGFLTATIKT
                     LDALQPKDRRRDHPRFNPDNLKRNVALLQPLESIAAAHKATPAQVALAWVLAQGPDVV
                     PIPGTKRRNYLEQNAAATGMALSAEEIARLAKMFPPGVASGTRYPEKQLAGLGI"
     gene            complement(1960..3150)
                     /locus_tag="VEM33_00705"
     CDS             complement(1960..3150)
                     /locus_tag="VEM33_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HYS50380.1"
                     /translation="MKGLLWLLVLSALAVALSLAMRGHGAYALFVLHPWRAEISLNLL
                     AILLVVVLAAGYFLLRVVWHAVRLPSHVRAFRQRRRDEKGRAAALGAIQALFEGQFVR
                     AEKLASGAAGLGATPGLASLLAARAAQRLRQFGRRDQWLERAKEGDGEWRLARLMTAA
                     ELLLEERRFLEARAVLRELNAGRPRHVAALLLSLRAEQGMANWDEVLRLANLLEKREA
                     MPPEALDSVRVNARVAILSARAADREGLTRHWEETPRSERLRPKIAAAAARAFIELGD
                     CRKAHRIIEEALEKDWDGALALLYGECTDEDAFERLERAERWLRERPGEAELLLTLGR
                     LCVQRELWGKAQSYLEASLATQPTRAAHVALARLFERIGRTDEANRHFRASADLGSAP
                     PASD"
     gene            complement(3147..4181)
                     /locus_tag="VEM33_00710"
     CDS             complement(3147..4181)
                     /locus_tag="VEM33_00710"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYS50381.1"
                     /translation="MENDTASASGTRGSSKLKAWRVSQAVTAFGLLALLFAWQWYDGH
                     NRIGALRDELAQRVRESESDARDARLAARQAQEAAREVQAKLAQIELKFAEFQNQQIG
                     LEALYEELSRNRDEWVIAEIDQILTIASQQLQLTGNVQVALSALQTAEARLARSGRSQ
                     FLPLRKVFARDIERLKTAPVLDVPGLAAKLDQLIAGVDSLPLVQDARPQATAAAKREA
                     EGLWERLVADLLGELKQLVRIENMGASDPALLSPPQAFFLRENLKLRLLNARLALLAR
                     DETAYRSDVKLAANWLERYFDTRSRAAAATAATLKQLASDSIRGSLPTIGESLAAVRA
                     YKPVRKQAGQ"
     gene            complement(4210..>4958)
                     /locus_tag="VEM33_00715"
     CDS             complement(4210..>4958)
                     /locus_tag="VEM33_00715"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYS50382.1"
                     /translation="GIVVTRPREHALGLAEQIRAAGADPILFPTIEILPTEDAAPVSS
                     LIARLDGFQLAIFVSPTAVMRGHATVVASRKWPEGLRVAAVGNGTASALEELGFHSVI
                     APSGDADSEALAELPELQDLQGRSVVIFRGQGGREWLRTRLEARGARVEYAECYRRAR
                     PEVDAGRLLARWQSGGIEAVSITSAEGLANLFEMLGPTGRGYLRATPVFVPHPRIALA
                     ARKLGVREIVVTGRGDVRTVAEMAAFFAKV"
BASE COUNT          862 a         1678 c         1570 g          848 t
ORIGIN      
        1 aagatcttga cctgcgaatc ggttcgatga acccggccac ggtccgcact tactttcttg
       61 atctgcagaa ccggcttgtc gcggagctgg agcgcatcga gggacgcagc ttccgcagcg
      121 atcagtggca gcggagtgaa gggggaggcg gcgagagccg catcatcgaa ggcggggacg
      181 tgttcgagcg cggcggggtg aatttctcca gggtgcacgg ggagcggctc ccgccctcgg
      241 cgagcgccgc gcggcccgag ctggccggcc gcgccttcga agccaccggc gtgtccctgg
      301 tgctgcatcc gcgcaatccc tatgttccga ccgttcacat gaacgtgcgg ttcctggtcg
      361 cgaccaagcc gcaaagcgag ccgacctggt ggttcggggg cggcatggac ttgacccctt
      421 actacgggtt cgaggaggac gcgcgccact ttcatgccac gtgccggcgg gcgctcgagg
      481 ctttcggctc cgactgctat ccccgtttca aacgctggtg cgacgaatac ttctatctca
      541 agcaccgcgg cgagccgcgc ggcatcggcg ggattttctt cgatgatttg aacgagagcg
      601 gtttcgactc gtgcttcgag ctcgctcgcc gcgtcggcga tcattttctt ccggcttatc
      661 ttcccatcat cgagcgccgc aaggatcttc cttacggcga gcgcgagcga gacttccagg
      721 cctatcgccg cggccgctat gtggagttca atctcgtcta tgatcgcggc acgctgttcg
      781 gattgcagtc ggccggccgc gccgagtcga tattgatgtc cctgcccccg ctcgtaaagt
      841 ggcggtatga ctggaaaccc gacccgtcga gcgcggaagg aaaactctat cgggatttcc
      901 tgcgtgccag ggactggctc ggcgaatgac tacggtgaga ggagcgcatg gacacacgca
      961 agcttggaac aaatgggccc gccgtctcgg cattgggtct gggctgcatg ggaatgacgg
     1021 gaatctacgg cgagccgaac gagcccgagt cgatcgcgac cattcaccgc gccatcgatc
     1081 tcggtgtcaa cctgatcgtc acttcggacg cgtacggggc gggaaacaac gaggaactgg
     1141 tcggtcgcgc gatcaaaggc agacgcaacc gggtgctcct cgccacgaaa ttcgggaacg
     1201 tgggcctgag cggcttcaag cttcccgaag gcctttcggc gggtcacccc gcatacgtgc
     1261 cgcaggcttg tgaggcctcg ctcaagcggc tcggcgtcga agtgatcgac atctacgggc
     1321 tgcatcgcgt cgaccccaag gtgccgatcg aggataccgt aggcgcgatg aagatgctgg
     1381 tcgaccaggg caaggtccgc catctcgccc tttccgaggc gggcgcggca accctccggc
     1441 gcgcgcacaa ggtgcatccg ctcgttgcgc tcgagaccga gtactcgcta tggtcgcgcg
     1501 atgtggaaaa ggacatcctc cccgcctgcc gcgagctcgg tatcgccttt atggcttact
     1561 cgccgcttgg ccgtgggttc cttaccgcga cgatcaagac gcttgacgcg ctccagccga
     1621 aggatcgccg tcgcgatcat ccgcgcttca atcccgacaa cctcaagcgc aatgtcgcgc
     1681 tgctgcaacc gctcgagtcg atcgccgcgg cccacaaggc gacaccggcg caggtggcgc
     1741 tcgcctgggt tctcgctcaa gggccggacg tagtgcccat cccgggaacg aaacggcgca
     1801 attacctcga acaaaacgcg gccgccaccg gcatggcctt gagcgcggag gaaatcgccc
     1861 gactcgcgaa gatgtttcct cccggcgttg cctcgggcac ccgctatccg gagaaacaac
     1921 tcgccggact cggcatttga acccgacctc gtcgggcttt cagtccgatg ccggtggcgc
     1981 cgatccgagg tcggcgctcg cacggaaatg ccggttggct tcgtcggtcc gcccgatgcg
     2041 ctcgaagagg cgcgcgagcg cgacgtgtgc cgcccgtgtc ggctgcgtgg cgaggctcgc
     2101 ctcgaggtaa ctttgagctt ttccccaaag ctcacgctgc acgcataggc ggcccagcgt
     2161 cagcaacaat tccgcctctc ccgggcgctc gcgcagccat cgttcggccc gctcgagccg
     2221 ctcgaacgcg tcctcgtcgg tgcattctcc atagagcagg gcgagcgcgc cgtcccagtc
     2281 tttctcgagc gcttcctcga tgattcggtg ggccttgcga caatccccca gctcgatgaa
     2341 ggcgcgcgct gcggcggccg cgatcttggg acgcaagcgc tcggagcgcg gcgtctcttc
     2401 ccaatgccgg gtaaggcctt cccgatcggc ggccctggcc gaaagtattg cgacgcgggc
     2461 gttgacgcgc acgctatcga gggcttccgg cggcatcgct tcccgtttct ccagcaagtt
     2521 cgccaggcgg aggacttcgt cccaatttgc catgccctgc tccgcgcgca gcgacagcaa
     2581 cagtgccgcg acatgccgag gccggccggc gttcagttcg cgcaggacgg ctcgcgcttc
     2641 gaggaagcgc cgttcctcca gcaggagctc cgccgcggtc atcaaacggg caaggcgcca
     2701 ctcaccgtcc ccttccttcg cgcgctcgag ccactgatcg cgccggccga attgccgcag
     2761 cctctgtgcc gcacgcgctg caagaagact cgcgagaccc ggcgttgcgc ccagcccggc
     2821 cgccccggag gcaagcttct cggcgcgcac aaattgaccc tcgaagagcg cctgaatcgc
     2881 ccccagcgcc gcagcgcgtc ccttttcatc ccgccgtcgt tgacgaaaag cacgcacatg
     2941 cgaaggaagc ctcacggcat gccacaccac tctcagcaga aagtaacccg ccgcgaggac
     3001 gacgaccaga agaatggcga gcaggttgag cgagatctcg gcgcgccacg gatgcagcac
     3061 gaacagcgcg taagcaccgt ggcctcgcat tgccagcgaa agggccacgg cgagcgccga
     3121 gagaacaagg agccaaagca agcctttcat tgcccggcct gcttccgaac cggcttgtaa
     3181 gcgcgcaccg ccgcgagact ttcgccgatc gtcggaagcg aaccccttat gctgtccgac
     3241 gcgagctgct tcagcgtcgc cgccgtcgcc gcggcagcgc gagaacgcgt gtcgaagtat
     3301 cgctcgagcc agtttgccgc gagcttcacg tcgctgcggt aggcggtttc gtcgcgcgca
     3361 agaagcgcga ggcgcgcgtt cagcaggcgc agtttcaggt tctcgcgcag gaaaaatgcc
     3421 tgcggcgggc tcagcagcgc cgggtcggag gctcccatgt tctcgatcct gacgagctgc
     3481 ttgagctcgc cgagcaaatc cgcgaccagc ctttcccata atccctcggc ctcacgcttg
     3541 gcggcggccg tcgcctgcgg gcgcgcgtcc tgcacgagcg gcagggaatc cacgcctgcg
     3601 atcaactgat ccagcttcgc ggcaagtcct ggaacatcca ggacgggcgc cgttttgagg
     3661 cgctcgatat cgcgggcgaa gaccttgcgc agcggcagga actgggagcg gccggagcgc
     3721 gccaggcgtg cttcggccgt ctgaagcgcg gacaatgcca cctggacgtt cccggtgagc
     3781 tgcagttgct gcgaagcaat ggtgagaatc tggtcgattt cggcgatcac ccattcgtcg
     3841 cgattgcgcg acaactcctc gtagagcgcc tcgaggccga tctgctgatt ctggaattcc
     3901 gcgaacttca actcgatctg cgcgagttta gcctgtacct cgcgcgccgc ctcctgtgcc
     3961 tgtctcgcgg cgaggcgcgc gtcgcgggcg tcggactcgc tctcgcgcac gcgttgagcg
     4021 agctcgtcgc gcagcgcgcc gatccggttg tggccgtcgt accactgcca agcgaagagc
     4081 agtgcgagca acccgaatgc ggtcaccgcc tgcgaaacgc gccaggcttt gagcttcgag
     4141 gatccccgag tcccggacgc cgatgcagtg tcgttttcca tacgtgagcc gactcccgat
     4201 gagaggcgat catactttag caaaaaaagc cgccatctca gcgacggtgc gaacatcccc
     4261 gcgcccggtc actacgatct cccgcacgcc gagtttgcga gcggccagcg cgatccgcgg
     4321 atgcggcacg aacaccgggg tcgcgcgcag gtatcccctg ccggtagggc cgagcatttc
     4381 gaagagattc gccaagcctt cggcgctggt aatcgagact gcttcgattc cgccgctctg
     4441 ccagcgggcg aggagccttc ccgcgtcgac ctcgggacgc gcacgccggt agcattcggc
     4501 gtattcgacc cgcgcgcccc gcgcttcgag tctcgttcgc agccattcgc gccccccctg
     4561 cccgcgaaag atcacgaccg agcgcccctg cagatcctgc agctcgggaa gttcggcgag
     4621 ggcctcgctg tcggcgtcac ccgagggcgc aatcaccgaa tggaacccca actcctcgag
     4681 cgcgcttgcg gtcccattcc ccacggctgc cacgcgcaag ccctccggcc atttgcggct
     4741 ggcgacgacc gtcgcatgtc cccgcatcac cgcggtcggg ctgacgaaaa tcgcaagctg
     4801 aaatccatcc aggcgcgcaa tcaggctcga aacgggcgcc gcatcctcag taggcaggat
     4861 ctcgatggtg ggaaagagaa tcgggtctgc gcctgccgcg cggatctgct ctgcaagccc
     4921 caaagcgtgc tcgcgcgggc gggtgaccac gataccgc
//
LOCUS       DASPZD010000038         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1062538, whole genome shotgun sequence.
ACCESSION   DASPZD010000038 DASPZD010000000
VERSION     DASPZD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062538"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VEM33_00720"
     CDS             complement(<1..241)
                     /locus_tag="VEM33_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50383.1"
                     /translation="MNSAVPVPDELWLRSRFAGPRNPKPVTEGDGHLWRSDREIRPAA
                     VLVPVVKREQGLTILFTRRTAHLNDHAGQISFPGGR"
     gene            443..688
                     /locus_tag="VEM33_00725"
     CDS             443..688
                     /locus_tag="VEM33_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50384.1"
                     /translation="MKIRFAALFGALMLVSASAFAFHCPADMKKIDEAMAKNPKLSAQ
                     QVADVKKFRADGETLHKAGKHQESVDTLAKAMKILGI"
     gene            complement(753..1733)
                     /gene="pip"
                     /locus_tag="VEM33_00730"
     CDS             complement(753..1733)
                     /gene="pip"
                     /locus_tag="VEM33_00730"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HYS50385.1"
                     /translation="MGELASFAVPGLFPEIEPRASGMLRLDAVHSMYWEESGDPGGIP
                     AVFLHGGPGAGSTPKHRRFFDPGAYRIIVYDQRGAGRSTPLGELRDNTTPHLVADLET
                     LRVHLRVERWVVFGGSWGSTLALAYAEAHPERCLALILRGIFLCRRSEIDWFLYGLRA
                     VFPEPWETFAGFLPREERGDLLRNYHSRLIDPDPAIHMPAARAWSIYEGSCSTLLPSA
                     ETVAYFAGDVVALGLARIEAHYFMNDIFLETNSLLENAHRLRDVPGVIVQGRYDMVCP
                     LVSAYELHRAWPKAEFRIVPDAGHSAWEPGILGALVDATQRFKRTPSLTG"
     gene            complement(1740..2714)
                     /locus_tag="VEM33_00735"
     CDS             complement(1740..2714)
                     /locus_tag="VEM33_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003275776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HYS50386.1"
                     /translation="MPKAIRIQQQGGPEVLKWEDVQVGDPGPGEARVRHKACGLNFLD
                     VYQRSGLYKIALPSGMGNEGAGVVEAVGAGVTHVKPGDRVAYAGGAPGAYSEVRTMPA
                     DRLVVLPEGISFETAAAMMLKGMTAQYLLRRTYKAQSGETILFHATAGGVGLIACQWL
                     KALGATVVGTAGSEEKCALAKAHGADHCVNYRKENFVERVKALTGGKGVPVVYDSVGK
                     DTFSGSLDCLQPRGLMVSFGNSSGAVPPFELVVLSQKGSLYITRPTLVTHTSKREDLV
                     ATAKDLFDVVLSGKVKIEVNHRYPLKDAAQAQRDLEARKTTGSTILIP"
     gene            complement(2774..3064)
                     /locus_tag="VEM33_00740"
     CDS             complement(2774..3064)
                     /locus_tag="VEM33_00740"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HYS50387.1"
                     /translation="MNQPPDPFEFLKNLWGPMGLPLAGVVAPTLVQDELDRRIAELRS
                     VENWLNMNLNVLRMTIQGLEMQKAGLAAMRNAAGSAPPDASDPGGREDPSKK"
     gene            complement(3098..>3297)
                     /locus_tag="VEM33_00745"
     CDS             complement(3098..>3297)
                     /locus_tag="VEM33_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYS50388.1"
                     /translation="VRHAHELPWTVRFLLRSIGAMNRNGSNLASYLLFESGYCRALID
                     LGYQDTMARRPEVLEFLGPRQ"
BASE COUNT          591 a         1047 c         1057 g          602 t
ORIGIN      
        1 agcggccgcc cggaaagctg atttgtccgg cgtggtcgtt gaggtgcgct gtacggcgcg
       61 tgaacaggat cgtcagcccc tgttcacgct tgaccaccgg caccagcacg gctgcgggcc
      121 ggatttcccg atcgctgcgc caaaggtggc cgtctccctc ggtcacgggt ttggggttgc
      181 ggggcccggc aaatcgcgag cgcaaccaga gttcgtcggg tactgggacg gctgaattca
      241 tttgacgaca ggcaaattgc cggtctttat tctacgcggc agcagtgccg ctatacgacg
      301 ggaaccgcaa ttgcattcga ccgccaccgg cgctattcta tcggcggccg gaatagattt
      361 gccggcgttg gcgtttacgt cgcaaggcgg gcggccggga ggcatgagcg gcccgcggat
      421 cgaaaccttt caaggagaac tcatgaagat tcgttttgca gcactattcg gcgcgctcat
      481 gctcgtaagc gcctccgcgt tcgcctttca ctgcccggcg gacatgaaga agatcgacga
      541 ggcgatggcg aagaacccga agctgtcggc gcagcaagtg gccgacgtca agaaattccg
      601 cgccgacggt gaaacgcttc acaaagcggg caagcaccag gaatcggtcg acacgctcgc
      661 caaggcgatg aagatactgg gtatctgaac cgcgttcccg aaacgtaaaa acgccgcggc
      721 ttgccgcggc gtttttgttt gtcttccgcc agtcagccgg tgagcgaagg cgtgcgcttg
      781 aagcgttggg tggcgtcgac cagcgcgccg agaatccccg gttcccaagc cgaatgcccg
      841 gcgtcgggca cgatccggaa ctcggccttg ggccaggccc gatgcagctc gtacgcggat
      901 acgagcgggc agaccatgtc atagcgtccc tgtacgatca ctcccgggac gtcgcggagc
      961 cggtgcgcgt tttcgagcag ggagtttgtc tccagaaaaa tgtcgttcat gaaatagtgc
     1021 gcctcgatcc gcgcaagacc cagggcgacg acgtccccgg cgaaatacgc cacggtctcg
     1081 gcactgggaa gcagggtcga gcaggatccc tcgtagatgc tccacgcgcg cgcagcaggc
     1141 atgtggatcg ccggatcggg gtcgatcaat cgactgtggt aattgcggag cagatcgccg
     1201 cgttcctccc gggggaggaa gcccgcgaat gtctcccagg gttcgggaaa caccgcgcgc
     1261 agtccgtaga ggaaccagtc gatctcgctc ctgcggcaaa ggaagatccc gcgcagaatc
     1321 agcgcgaggc aacgctccgg gtgcgcctcc gcgtaggcga gcgccagcgt gctgccccag
     1381 gagcctccga acacgaccca gcgttccacg cgcaaatgca cgcgcaaagt ctccagatcg
     1441 gcgaccagat gcggcgtggt gttgtcgcgc agttccccga gcggggtgga gcgccccgcc
     1501 ccgcgctggt cgtacacgat gatgcgatac gcgcccgggt cgaaaaaacg ccggtgcttc
     1561 ggcgtgctgc ccgcacccgg gcccccatgc aggaaaaccg cggggatacc gcccggatcg
     1621 ccgctctcct cccagtacat cgaatgaacg gcatcgagcc gcagcatgcc gcttgcgcgc
     1681 ggctcgattt ccggaaacag gcccgggacc gcgaaactcg ccaactcgcc catgggcttc
     1741 tacgggatca ggatggtcga tccggtcgtc ttgcgcgctt cgaggtcgcg ctgtgcctgt
     1801 gccgcgtcct tgagcggata acggtgattc acctcgatct tcactttgcc tgagagcacc
     1861 acgtcgaaca gatccttcgc ggtcgctacg agatcttcgc gctttgaggt gtgggtgacc
     1921 agggtcgggc gcgtgatgta gagcgagccc ttctgcgaga gcaccaccag ctcgaacggg
     1981 ggaaccgctc ccgaggagtt tccgaagctc accatcaatc cgcgcggctg caggcagtcg
     2041 agcgacccgc tgaaggtgtc ttttccgacc gagtcgtaca cgacaggcac gcccttgccc
     2101 ccggtgagcg ccttgactcg ttcgacaaaa ttctccttgc ggtagttgac gcagtggtcg
     2161 gcgccgtgcg ccttggcgag cgcgcatttc tcctccgaac cggcggtgcc gactaccgtg
     2221 gcgccgagcg ccttcaacca ctggcacgcg atcagtccga ctccccccgc tgtcgcgtgg
     2281 aacaggatgg tctcgcccga ttgagccttg taggtccgcc tcagcaggta ctgggcggtc
     2341 atgcctttca gcatcatcgc ggctgccgtt tcgaaggaaa taccctccgg caggacgacg
     2401 aggcggtcgg ccggcatggt gcgcacctcg gaataggcgc cgggggcgcc gccggcataa
     2461 gcgacgcggt cacccggctt gacgtgcgtg acccccgccc ccacggcctc caccacaccg
     2521 gccccttcgt tgcccatccc cgaaggaagg gcgatcttgt acagtccgga gcgctggtag
     2581 acgtcgagaa aattgagccc gcaagccttg tggcgcaccc gcgcctcgcc cggcccgggg
     2641 tccccgacct ggacgtcttc ccatttcaga acttcggggc cgccttgttg ttggattcga
     2701 atcgctttgg gcattgcttc tcctcggtca gtgatgggtg ccagggtctc ctggcgcgtt
     2761 tcgggtgcgg ggactatttt ttagacggat cttcgcggcc gcccgggtct gaagcatccg
     2821 gaggcgcgct cccagcggcg tttcgcatcg cggcaagacc ggctttctgc atttccagcc
     2881 cctgaatggt catgcgcaga acgttgaggt tcatgttgag ccagttctcc accgatctca
     2941 gctctgcaat gcgcctgtcg agctcatcct ggacgagcgt cggggccact actcccgcga
     3001 gcggcaggcc catcgggccc cagaggtttt tcaggaattc gaacggatcg ggaggctggt
     3061 tcatgagcgc ggggccacga tgagggatat ttcaagttta ttgtcgggga cccaaaaact
     3121 ccagcacctc ggggcggcgc gccatggtgt cctgatagcc cagatcgatc agcgcccggc
     3181 agtagccgct ttcgaagagc agatagctcg cgaggttcga tccgttgcgg ttcatcgcgc
     3241 cgatgctccg caggaggaag cgcaccgtcc atggcagctc gtgtgcgtgc cgaacga
//
LOCUS       DASPZD010000039         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_219263, whole genome shotgun sequence.
ACCESSION   DASPZD010000039 DASPZD010000000
VERSION     DASPZD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219263"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VEM33_00750"
     CDS             <1..668
                     /locus_tag="VEM33_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS50389.1"
                     /translation="LREALTVHERPRDAEIAGRIRELLEQVSLKESKLANFPSELSGG
                     EKRRVGIARVLAVRPRLIVADEPTSALDISIQAQVVNLLRDLQKNLGLTYLFISHDLP
                     VVELVSQKVAVMYLGRIVEMGLAERLAHQPRHPYTGILWSSLADKQTTEVTRRVTDVA
                     VERERRSWGVFDFEQPTSGCRFAPRCPLYKDKGRPAICTDAASEPQLRDVGHGHVVAC
                     HFA"
     gene            complement(692..>2363)
                     /locus_tag="VEM33_00755"
     CDS             complement(692..>2363)
                     /locus_tag="VEM33_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYS50390.1"
                     /translation="HTRLNTISLSGTKVPKVALPGFEDHGEILKWRMRENLPGSFPFT
                     AGVFSFKRENEDPTRMFAGEGDPFRTNQRFLLLSKDMPAKRLSTAFDSVTLYGFDPDL
                     RPDINGKVGNSGVSIATLEDMKVLYSGFDLCDPATSVSMTINGPAPTILAMFLNCAID
                     QQIDEFRESNARDPTAAERGEIRERTLSAVRGTVQADILKEDQGQNTCIFSTEFSLKV
                     MGDIQEYFIEHDVRNFYSASISGYHIAEAGANPISQLAFTLANGFTFVEAYLARGMRI
                     DDFAPNLSFFFSYGMEPEYAVLGRVARRIWAIAMRDKYGANERSQKLKFHSQTSGRSL
                     HAQEIAFNDIRTTLQALISTYDNTNSLHTNAYDEAITTPTEESVRRAMAIQLVINREW
                     GLGKNENPNQGSFIVETLTDLVEEAVLKEFEAIAERGGVLGAMETGYQRGKIQEDSML
                     YEHRKHDGSYPIIGVNTFRNPRGNPVPQKLELIRSSEEEKQSQLRRLAEFHDRHKVES
                     GPMLKRLQQSVIKDENVFAVLMDAVRCCSLGQITSALFEVGGQYRRNM"
BASE COUNT          393 a          732 c          780 g          458 t
ORIGIN      
        1 tcctgcgcga ggcgctcacc gtgcacgagc gcccgcgcga tgccgaaatc gcaggccgca
       61 tccgcgagct gctcgagcag gtgagtctca aggagagcaa gctcgcgaat tttccgtcgg
      121 agctttccgg cggcgagaag cgccgagtgg ggatcgcgcg cgtgctcgcg gtgcgcccgc
      181 gcctgatcgt ggccgacgag ccgacgagcg cgctcgacat ctcgatccag gcgcaggtgg
      241 tgaacctgct gcgcgacctg cagaaaaacc tggggctcac gtatcttttc atctcgcacg
      301 atctcccggt cgtcgagctg gtcagccaga aagtcgcggt catgtatctc gggcgcatcg
      361 tcgagatggg cctggcggaa aggctcgcgc accagccgcg ccatccctac acgggcatcc
      421 tgtggtcgtc gctcgctgac aagcagacca cggaggtgac ccggcgcgtg accgacgtgg
      481 cggtcgagcg cgagcgccgc agctggggcg tgttcgactt cgagcagccg acaagcggct
      541 gccgcttcgc cccgcgctgc ccgctgtaca aggacaaggg ccgcccggcg atctgcacgg
      601 acgccgcgtc cgaaccgcaa ttgagggacg tggggcacgg gcacgtggtg gcgtgccact
      661 tcgcgtaggc tgaggagatg ctctccccgc cctacatgtt gcggcggtat tgccctccca
      721 cctcgaacag cgccgaggtg atctggccga gggagcagca gcgcaccgcg tccatcagga
      781 cggcaaagac gttctcgtcc ttgatcaccg actgttgcaa acgcttcagc attgggccgg
      841 actcgacctt gtgccggtcg tgaaactcgg ccagacgcct cagctgagat tgtttttctt
      901 cctcgctcga gcggatcagc tccagtttct gcgggaccgg gttgccgcgg ggattgcgga
      961 aggtgttgac gccgatgatc ggatacgagc cgtcgtgctt cctgtgctcg tagagcatcg
     1021 aatcttcctg gatcttgccg cgctggtatc cggtctccat cgcgccgagg acgccgccgc
     1081 gctcggcaat cgcctcgaac tccttcagca ccgcctcctc gacgagatcg gtcagggtct
     1141 cgacgatgaa cgagccctgg ttcgggttct cgttctttcc caacccccat tcccggttga
     1201 tgacgagctg gatcgccatg gcgcggcgca ccgactcctc ggtgggcgtc gtgatggcct
     1261 cgtcgtatgc gttggtgtgc agcgagttgg tgttgtcgta cgtcgagatc agcgcctgca
     1321 gggtagtgcg gatgtcgttg aacgcgatct cctgggcgtg cagcgagcgc ccggaggtct
     1381 gcgaatggaa cttgagcttc tggctgcgct cgttcgcgcc atacttgtcc cgcatcgcta
     1441 tcgcccagat gcgccgcgcc acgcgcccga gcaccgcgta ctcgggctcc attccgtagc
     1501 tgaagaaaaa cgacaggttg ggcgcaaaat cgtcgatcct cattccgcgt gcaagatagg
     1561 cttcgacaaa agtgaacccg ttggcgagcg taaaggcgag ctgcgagatc gggttcgcgc
     1621 ccgcctcggc gatgtgatag cccgagatcg aggcggagta gaaattgcgc acgtcgtgct
     1681 cgatgaagta ctcctggatg tcgcccatca ctttgaggga gaactcggtc gagaagatgc
     1741 aggtgttctg cccctggtct tccttgagga tgtccgcctg cacggtgccg cgaaccgccg
     1801 acagcgtccg ctcccggatt tccccgcgct cggcggccgt cgggtcgcgg gcattgcttt
     1861 ctctgaactc gtcgatttgc tgatcgatcg cgcagttgag gaacatcgcg aggatcgtgg
     1921 gcgccggccc gttgatcgtc atgctgacgc tcgtcgccgg atcgcacagg tcgaagcccg
     1981 agtagagcac tttcatgtct tcgagcgtgg cgatggacac gcccgagttg ccgaccttgc
     2041 cgttgatgtc cgggcgcagg tccgggtcga agccgtagag cgtcaccgaa tcgaacgcgg
     2101 tcgatagtcg tttcgccggc atgtctttcg agagcagaag gaagcgttgg ttggtgcgga
     2161 acggatcgcc ctcgcccgcg aacatgcgcg tcgggtcctc gttctcgcgc ttgaaggaga
     2221 atacgcctgc ggtgaacggg aaggagccgg gcaggttctc gcgcatgcgc catttcagga
     2281 tctcgccatg gtcctcgaag ccgggcagcg cgactttcgg aaccttggtc cccgaaaggg
     2341 aaatcgtgtt gagccgggtg tgg
//
LOCUS       DASPZD010000040         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_821720, whole genome shotgun sequence.
ACCESSION   DASPZD010000040 DASPZD010000000
VERSION     DASPZD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821720"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2187
                     /locus_tag="VEM33_00760"
     CDS             <1..2187
                     /locus_tag="VEM33_00760"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYS50391.1"
                     /translation="RVDSTRSRAMAGVVGVYSGADCLADGLGPIPHNPLPSTREDMKL
                     TGPGGGRIFEGPHWLLPTDKARHVGEAVAMVVAETRDEALDAVEALEVEYEELPWVIH
                     SERAVEEGAPRAWDEVPGNVIVDTHFGDREATDRAFAEAGHVVRMKFDIARCTAVAIE
                     PRAALASYDARTGRYTLYAGGGGAVRYKKDLASLLGIEPRNLRVVCRDVGGNFGSKNR
                     IYVEYGLALWASRKLGCPVKFTASRSEAFLSDYQGRDLVTEVELALRRDGKFLAMRAS
                     NLSNVGARCVSLSPLSKGSGLITGCYDIPAASLRSRAVFTTTAPTNPYRSSGRPEVNF
                     AIERLIDTAAAELGMDRITLRRNNLVRPEAMPYRNAVGMTYDSGTYEANMDLAMRIAD
                     WNGFEARRESAAVRRRLLGRGLSNYVESSIGSPKERAEISITPAGRVRVVIGTQPSGQ
                     GHETSFAQVVSSLLSVPVEAIDILIGDTDVVSVGGGSHSGRSMRHAATVFSLAARELV
                     EKGKRIAAHMLEVGAAEIRFADGRFSAPHALRAYDFLELARDSAEALLPRELSGGLAA
                     VADNEMHDPVFPNGCAICECEVDPETGAVDVTRFTAVDDVGRCINPLIVHGQTHGGAA
                     QGIGQAISEIYHVDPETGQPHTGSLMDYAIPRSDTLPSFSAEIVEVLSPTNPLGIKAA
                     GEGATTPAPAAVVNAIVDALAGLGVRDITMPATPENIWRAIQEAKK"
     gene            2184..3146
                     /locus_tag="VEM33_00765"
     CDS             2184..3146
                     /locus_tag="VEM33_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50392.1"
                     /translation="MNAGRLFAALALLFTALCAFGQSYPKGPVRVIVPFPAGGGVDTA
                     ARILAQKLAEGFGKPFVIENRGGANGNIGTEAAAKSANDGATLLFTGAGFVTNPSLYR
                     SVPYDPARDFDPISLMALGPNVLVVHPSLPARSVKDLIALAKSQPGRIGFAGSGSGST
                     PHLAGELFNVMAGVQLVHVPYRGSGPAMIGLLAGEAPVMFLPAINAGGHVAAGRLRAI
                     AVTSRERLQAMPDLPTVSEAGLPGYESSQWYGLLAPAGTPLDVLNLLNAQVANIMQSA
                     DMKTRMTNDGLVPIGGSREQFTAHIRSETEKWARVISASGARVD"
     gene            3147..4019
                     /locus_tag="VEM33_00770"
     CDS             3147..4019
                     /locus_tag="VEM33_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYS50393.1"
                     /translation="MSELDFTKPEKPAKPPVEAPAKPSIYDPAMAMEFFGSAGKAETI
                     AQGTTIFTENEKGNRLLLRRDRMYLLLDGEVSLIAKNKAIGRIGKGEIFGEMTSISEM
                     PRSATAVAKIPCRVIGLDDSQFQSALRKKPEFALMLMSVMIRRMRDAIGKHNPGGPAS
                     AGKMKESAVFDKSALAILSRALSQTTVRYERNKVIVKEGQAGLMMYVVLEGRLAASIQ
                     DKIVEKIGPGGVFGEMALVERTPRLATVISETDCGLLAFGRNTLLDLIKASPDFAVSL
                     LGAVGDRARFIASR"
     gene            complement(4016..>4121)
                     /locus_tag="VEM33_00775"
     CDS             complement(4016..>4121)
                     /locus_tag="VEM33_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HYS50394.1"
                     /translation="GHAIPNFYFHVTTAYDILRHNGVELAKRDYLANP"
BASE COUNT          748 a         1344 c         1369 g          660 t
ORIGIN      
        1 cgcgtcgact cgacccgctc gcgggcgatg gcgggcgttg tcggcgtgta cagcggcgcg
       61 gattgcctgg ccgacggtct cggcccgatt ccgcacaatc ccctcccctc gacccgggaa
      121 gacatgaagc tcacgggccc cggcggcggc aggatcttcg aaggccccca ttggctgctg
      181 ccgacggaca aggcgcgcca cgtgggcgaa gccgtcgcga tggtcgtcgc cgagacgagg
      241 gacgaggcgc tcgacgccgt agaagcgctc gaggtcgagt acgaggagct gccctgggtc
      301 atccactccg agcgcgccgt cgaagaggga gccccgcgcg catgggacga ggtgcccggc
      361 aacgtgatcg tcgacaccca tttcggcgac cgcgaggcga ccgaccgcgc gttcgcagaa
      421 gccggccacg tcgtcaggat gaaatttgac atcgcgcgat gcactgcggt tgcgatcgaa
      481 ccgcgcgcgg cgctcgcatc ctacgacgcg cgcacgggcc ggtacacgct ctacgcggga
      541 ggcggcggcg ccgttcgcta caagaaagac ctcgcttcgc tcctcggaat cgagccgcga
      601 aacctgcgcg tcgtctgccg cgacgtcggc ggcaacttcg gatcgaagaa tcgcatatac
      661 gtcgaatatg gcctcgcgct ctgggcctcc cgcaagctcg ggtgcccggt caagttcacc
      721 gcctcgcgat ccgaagcctt cctctccgac tatcaggggc gggacctcgt caccgaggtc
      781 gagctcgcgc tccgccgcga cggaaaattt ctcgcgatgc gagcctccaa cctctccaac
      841 gtcggcgcgc gctgcgtttc gctctcgccg ctttcgaaag gatccggcct tatcaccggc
      901 tgctacgaca ttcccgccgc cagcttgagg agccgcgctg tcttcacgac cacggcgccg
      961 acgaatccct atcgcagctc cggacggccc gaggtcaact tcgcgatcga gcgcctgatc
     1021 gacacggccg ccgcggagct cggcatggac cggatcaccc tgcgccgaaa caacctcgtc
     1081 cgcccggaag cgatgcccta ccggaacgcg gtgggaatga cctacgacag cggcacttac
     1141 gaggccaaca tggaccttgc gatgcgaatc gcagactgga acggtttcga agcccgccgc
     1201 gagagcgccg ccgtgcgccg caggctcctc gggcgcggcc tctcgaacta cgtcgaatcc
     1261 tcgatcggct cacccaaaga gcgcgccgaa atcagcatca ctcccgcggg gcgcgtgagg
     1321 gtcgtgatcg gcacgcagcc atcggggcag ggtcacgaga cgagctttgc gcaggtggtg
     1381 tcttcgctgt tgtcggtacc cgtggaagcg atcgatatcc tgatcggcga caccgacgtg
     1441 gtgagcgtag gcggcggctc gcattccggc cgctcgatgc gccacgcggc gacggtgttc
     1501 tccctggccg caagggaact cgtcgaaaag gggaagagaa tcgcggctca catgctggag
     1561 gtcggcgcgg cggagatacg gttcgctgac gggcgcttct ccgcgcctca cgcgctcagg
     1621 gcgtacgact ttctggaatt ggcgcgagac tcggcggaag ccctcctgcc cagggaattg
     1681 tcgggcggac tcgccgccgt cgccgacaac gagatgcacg atccggtatt cccgaacggc
     1741 tgcgcgatct gcgagtgcga ggtcgacccc gaaacagggg cggtcgacgt gacgcgattc
     1801 accgcggtcg atgacgtcgg tcgctgcatc aatcccctga tcgttcacgg ccagacgcac
     1861 ggcggcgcag cccaagggat cggccaggcg atctccgaga tctatcacgt cgacccggaa
     1921 acggggcagc cgcacaccgg gtcgctgatg gactacgcca ttcccagatc ggatacgctg
     1981 ccctccttca gcgccgagat cgtggaagtt ctttcgccga ccaacccgct cggaatcaag
     2041 gccgcgggcg aaggcgcaac cacgccagcg cccgccgcgg tcgtcaacgc catcgtcgac
     2101 gcgctcgccg gtttgggcgt gcgcgacatc accatgcccg ccacgccgga aaacatatgg
     2161 cgggcgattc aagaagccaa aaaatgaacg ccgggcgcct gtttgcggcc ctcgcgctgc
     2221 ttttcaccgc gctctgcgcc ttcggccaga gctacccgaa aggtccggtg cgcgtgatcg
     2281 tgcccttccc agccgggggc ggcgtggata cggcggctcg catcctcgcg cagaagctcg
     2341 ccgagggttt cggcaagccc ttcgtgatcg aaaaccgcgg aggcgccaac ggaaacatcg
     2401 ggacggaagc cgctgcgaaa tcggcgaacg acggcgctac gctgctgttt acgggcgccg
     2461 gcttcgtcac caacccgagt ctctacagga gcgtccccta cgacccggcg agggatttcg
     2521 atccgatcag cctgatggcc ctcggaccca acgtgctcgt cgtgcatccg tcgctgccgg
     2581 ccagatcggt caaggacctc atcgctcttg ccaagtcgca gcccgggcga atcgggtttg
     2641 ccggctcagg ctcgggcagc acgccgcatc tcgcgggcga gctcttcaac gtgatggccg
     2701 gcgtgcaact ggtgcacgtt ccgtaccggg gttcgggtcc ggcgatgatc ggcttgcttg
     2761 cgggagaggc tccggtgatg ttcctccccg cgatcaacgc cggcggacac gtcgcggcgg
     2821 gccgccttcg cgcgatcgcg gtcacgagcc gcgagcggct gcaggcgatg ccggacctgc
     2881 cgaccgtttc cgaggccggt cttccgggct acgaatccag ccagtggtac gggctgctcg
     2941 cgccggcggg aacgcctctt gacgtgctca atctgctcaa tgcgcaagtt gcgaatatca
     3001 tgcaaagtgc cgacatgaaa acccgcatga cgaacgacgg cttggtgccg atcggaggca
     3061 gccgcgagca attcacggcc cacatcagga gcgagaccga gaagtgggcg cgggtgatca
     3121 gcgcttcggg agcgagggtg gattgaatga gtgaactcga tttcacaaag cccgagaagc
     3181 cggcaaaacc tccggtcgag gcgcccgcga agccgtcgat ctacgacccg gcgatggcaa
     3241 tggagttttt cggctcggcc gggaaggccg agaccatcgc gcaagggacg accattttca
     3301 ccgagaacga gaaaggcaac aggctcctgc tccggcgcga caggatgtac ctgctcctgg
     3361 atggcgaggt cagtctgatc gcgaagaaca aggccatcgg caggatcggg aagggcgaga
     3421 tcttcggcga gatgacttcc atcagcgaga tgccccgcag cgcgaccgcc gtggcgaaaa
     3481 taccgtgccg ggtcatcggg ctcgacgaca gccagtttca aagcgccttg cggaaaaagc
     3541 cggagttcgc gctcatgctg atgagcgtca tgattcgccg catgcgcgat gcgatcggca
     3601 aacacaatcc gggcggcccc gcctcggcgg gcaagatgaa ggaatccgcg gtattcgaca
     3661 agtccgcgct cgccattctc tctcgcgcgc tttcccagac gacagtgcgc tacgagcgta
     3721 acaaggtgat cgtgaaggaa ggccaggccg ggttgatgat gtacgtcgtg ctggagggcc
     3781 gccttgccgc atcaatccag gacaagatcg tggagaaaat cgggccgggc ggcgtgtttg
     3841 gcgaaatggc gctggtcgag cgcacgccgc gccttgccac cgtgatatcg gaaacggatt
     3901 gcgggctgct cgcgttcggc cggaacaccc tgctcgacct gatcaaggcg agccccgatt
     3961 tcgcggtgtc gctcctgggc gcggtgggcg accgggcgag gttcatcgcc tcgcgctagg
     4021 ggttggcgag gtagtcgcgc ttggcgagct ccacgccgtt gtggcggaga atgtcgtagg
     4081 cggtcgtgac gtgaaagtag aagttcggaa tcgcgtggcc g
//
LOCUS       DASPZD010000041         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_820978, whole genome shotgun sequence.
ACCESSION   DASPZD010000041 DASPZD010000000
VERSION     DASPZD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820978"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1609)
                     /locus_tag="VEM33_00780"
     CDS             complement(209..1609)
                     /locus_tag="VEM33_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYS50395.1"
                     /translation="MPPASRRPRSRQPELFPRSKRSIIEIAPNHRLVQITEEVDWTEL
                     EELVQAIRLSKLKNAAGRPPHLRALIGALMLRALRHRPLRDLEDLIRYYAPARYLCGL
                     TETEWSPDFNTISDFEQLMGEEGVKLINEHVVKWAVEEKLADPTVVVADTTAQEAAIP
                     YPNEMNMMATFVSAIAAASKNVGATLKGFVKRAGSLFQTAKKKLREFRLFAKDKSKAA
                     RMKLTSAMANIVEQVQDGLELALDEASRQKMRLTKHRKRAWAKLRKLQATMTTLLPQI
                     RYWIKTGWVAANKVVSLYIPEIYSIVRGKVGKNVEFGLKWGMTRLRGGFVLATLGKEK
                     NELVDSDFAVRAAKDAKALFGKAPKSYAYDRAGWGEANIAELKQLGVREVGLAPRGKA
                     KWLVAGKVKDKLVSERAQVEGCIGSVKSRKYGFNKPAAKSAAGMGVCGQRSVLGFNLN
                     KLMNGLAERRRVVLVG"
     gene            1742..1879
                     /locus_tag="VEM33_00785"
     CDS             1742..1879
                     /locus_tag="VEM33_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50396.1"
                     /translation="MKPLVSPQEVDVQQIKITDSPNEDSDDVVDEDIGVRVNDLVAQK
                     T"
BASE COUNT          348 a          692 c          579 g          437 t
ORIGIN      
        1 cggcgtcctg ccagaaccgg atgagctcga agtttccttg tttgggccag gggtcggtga
       61 gtgtgtcgtc gtacatattt acctagttag agcctgcccg gaaatttggc agctgcccgg
      121 tgcgcctcac cgacgctgca agcattgatt ttgctgggcg atccgaatcc ggcagcgggc
      181 ggcttggttt gcagtagtcc acatctgatc acccgaccag caccaccctt cgccgctccg
      241 ccaacccgtt catcagcttg ttcaagttga atcccaagac ggagcgttgt ccgcagactc
      301 ccatccccgc cgccgatttc gcggcgggct tgttgaaccc gtactttcgt gacttcaccg
      361 agccgataca tccttccact tgtgctcgtt ccgaaaccag cttgtccttg accttgcccg
      421 cgaccagcca cttcgccttg ccccgggggg ccaggcccac ctcgcggact cccagctgct
      481 tcaactcggc gatgttggcc tcgccccagc cagcgcggtc gtatgcgtag gacttcggcg
      541 ctttcccaaa cagcgccttg gcgtccttcg ccgcacgcac cgcgaagtcc gagtcgacca
      601 gctcgttctt ttccttcccc agggtggcca gtacgaagcc gccacggagt cgggtcatgc
      661 cccacttcaa gccaaactcg acgttcttgc cgaccttgcc gcgaacgatc gagtagatct
      721 cgggaatgta taggctcacg accttgttgg ccgcgaccca gcccgtcttg atccagtagc
      781 ggatctgggg cagcaacgtc gtcatcgtcg cctgcagctt tcgaagtttc gcccaagcgc
      841 gcttgcgatg cttcgtgagc cgcatcttct gtcgggacgc ctcgtccaag gccagttcga
      901 gcccgtcctg cacctgctcg acgatgttcg ccatcgccga cgtcaacttc atccgcgcgg
      961 ccttcgactt gtccttcgcg aacagtcgga attcccgcag cttcttcttc gcggtttgaa
     1021 acaggctgcc ggcacgcttc acgaagccct tgagcgtcgc tccgacattc ttgctcgcag
     1081 cggcaatcgc cgagacgaac gtcgccatca tgttcatctc gttcgggtac gggatcgccg
     1141 cctcctgcgc cgtcgtgtcc gcgaccacca cggtcggatc ggcgagcttc tcctccaccg
     1201 cccacttcac cacgtgctcg ttgatcagct tgaccccctc ttcccccatc agctgctcga
     1261 agtccgagat cgtgttgaaa tccgggctcc actcggtctc ggtcaacccg cagaggtatc
     1321 gcgccggcgc gtagtaccgg atcagatcct ccagatcgcg gagcggccgg tggcgcagcg
     1381 cacgcaacat caacgctccg atcagcgctc gaagatgagg cggccggccg gccgcgttct
     1441 tgagcttgct gaggcggatc gcctgcacca gctcctcgag ctcggtccag tcgacctcct
     1501 cggtgatctg gacgagccga tggttcggcg cgatctcgat gatggatcgc ttcgaacgcg
     1561 gaaataactc gggctgccgg ctgcgtggcc ggcgtgacgc tggtggcatc ggtggtatcc
     1621 tcgagtcgca cgggtgctcg agaagatcgt cgccggtacc tctgacattc cgcccgggag
     1681 ggccccacaa ttgccgggca ggctctagtc tagttccgtg cgttgcatgg tctccgcttc
     1741 cgtgaagcct ctcgtatcgc cgcaagaggt cgatgtgcag cagatcaaga tcaccgacag
     1801 cccgaacgaa gattccgacg atgtcgtcga cgaggacatc ggcgtacgcg tgaacgacct
     1861 cgtcgcccag aagacgtgaa atgtagttag agtcgcagaa tttacgttat ggcaactgcc
     1921 atttatagct tgcgatcatc agtgccggat tcgattcatc acgcgcgggc gagccattgc
     1981 cctactcccg agcgagccgc catgcgccct ccttcggacg gatacacttt cctgagacat
     2041 ggggcggccg gtcgtc
//
LOCUS       DASPZD010000042         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1436715, whole genome shotgun sequence.
ACCESSION   DASPZD010000042 DASPZD010000000
VERSION     DASPZD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436715"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..686)
                     /locus_tag="VEM33_00790"
     CDS             complement(45..686)
                     /locus_tag="VEM33_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50397.1"
                     /translation="MPVDDTGTIQKSIDCRILGGPRRNLATLLPAIDGVGTEFTGNNQ
                     AWHGNRYDVGIHRLPAETEPMTLERTPGEYLRFPSPGEVLAIERAARRARAKAIGRLF
                     AVAGRQLKEMIMKVPRTDPAPHEGSRFPTKRKTMTTSFWKDALASLPPSVQVRYAASF
                     EAAEHFDALLDLGSEAWGSAKQGLAKICQASARAMRRTARILEGAAHRLLPTR"
     gene            791..1537
                     /gene="surE"
                     /locus_tag="VEM33_00795"
     CDS             791..1537
                     /gene="surE"
                     /locus_tag="VEM33_00795"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HYS50398.1"
                     /translation="MRILLSNDDGYFAPGIAVLAEALSDLASVTVVAPERDRSGASNS
                     LTLDRPLSVRTSANGFRFVNGTPTDCVHLAVTGLLEEMPDMIISGINLGANMGDDTIY
                     SGTVAAATEGYLLGIPSLAISLASKAGKHYATAARVTLELVDRFQKRPPREPVLLNVN
                     VPDVAYDALKGFEVTRLGRRHKAEPMIKTKTPRNETVYWVGAAGSAQDAGPGTDFHAV
                     ASDCVSLTPLQMDLTHQQQIPAIKTWLAAK"
     gene            1537..2193
                     /locus_tag="VEM33_00800"
     CDS             1537..2193
                     /locus_tag="VEM33_00800"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYS50399.1"
                     /translation="MLPKTRGIGMTSQRTRDRMVARLREKGIRDEAVLAAMAATPRHI
                     FIEEALASRAYEDTALPIGFEQTISQPFVVARMIEALRGEAPLGNVLEIGTGCGYQAA
                     LLARLATEVYSVERIAGLLEKARGNLRPLRLSNLRLVHADGTHGLPEAAPFDGIIVAA
                     AAPRVPGALLQQLAPGGRMIVPVGSGDQALCLIERAPSGFTEKWLDAVRFVPLRGGKQ
                     "
BASE COUNT          397 a          709 c          708 g          407 t
ORIGIN      
        1 gtccaggcgc ggagcgggct tttttttggg tcgggatgcc cggctcaacg cgtgggcagc
       61 agccggtgcg cagccccctc gagaatgcgc gccgtcctgc gcatcgcgcg cgccgaggct
      121 tgacagatct tcgcaaggcc ctgcttggca gaaccccacg cttcgctgcc caggtcgagc
      181 agcgcgtcga aatgctcagc ggcctcgaaa ctcgccgcgt agcgaacctg cacgctgggc
      241 ggcaaggaag caagggcgtc tttccagaac gatgtagtca tggtcttcct cttggttgga
      301 aatcgcgatc cctcgtgcgg cgcagggtcc gtgcgaggga ccttcatgat catctccttc
      361 agctggcgcc cggctacggc gaacaggcgg ccaatggcct tcgcccgtgc gcgtctcgca
      421 gctcgttcga tcgccagcac ttcccccggc gacggaaagc gaagatattc ccccggggtt
      481 ctctccagtg tcatcggttc tgtttcggca ggcaatctat gtatgccgac gtcatagcga
      541 tttccgtgcc acgcttgatt attccctgtg aattcagtgc cgacgccgtc aatagcaggc
      601 aaaagcgtcg ccaaattgcg acgcggcccg ccgaggattc tgcaatcgat tgatttctgg
      661 attgttccgg tgtcgtcgac cggcaacaac cgggaggatg aatcggcccg gctggtagaa
      721 tgcgtcgcgt gtgcccgcgc ttccaggcaa tcggactctg atctttcgat acgcaagggt
      781 tggagtaaaa gtgcgcattc tgctcagcaa tgacgacggg tacttcgcgc ccggcatcgc
      841 agtcctagca gaggcgctct ccgatcttgc gagcgtcacc gtagtcgcgc ccgagcgtga
      901 ccgcagcggc gcctccaact cgctcaccct ggaccggccg ctttcggtgc gcacgtccgc
      961 gaacggcttc cgattcgtaa acggcacgcc gacggactgc gttcacctcg cggtcaccgg
     1021 gttactcgag gagatgcccg acatgatcat ctcgggcatc aatctgggtg cgaacatggg
     1081 cgatgacacc atctactcgg gcaccgtcgc agcggcaacc gagggctatc tgctcggcat
     1141 cccgtcgctg gcgatctcgc tcgccagcaa ggcgggaaag cactacgcca cggcggcccg
     1201 cgtcacgctc gagctggtcg atcgctttca gaagcggccg ccacgcgagc ccgtattgct
     1261 caacgtgaac gttcccgatg ttgcctacga cgcgctgaag ggtttcgaag taacgcggct
     1321 cggccgacgc cacaaggccg agcccatgat caagaccaag actccccgca acgagacggt
     1381 gtactgggtg ggggccgcgg ggagcgcgca ggacgccggg ccggggaccg attttcacgc
     1441 tgtcgcgtcc gactgcgtgt cgctcacacc gctgcagatg gaccttacgc atcagcagca
     1501 gattcccgcg atcaaaacgt ggctggcggc gaaatagtgc tcccgaaaac ccgcggaatc
     1561 gggatgacct cgcagcgcac gcgcgaccgg atggtggcgc gcctgcggga gaagggaata
     1621 cgcgacgagg ccgtgctcgc cgcgatggcc gccactccgc gccatatttt catcgaggaa
     1681 gcgctcgcga gccgcgccta tgaggatacc gccctgccga tcggcttcga gcagaccatc
     1741 tcgcaacctt tcgtggtggc gcgcatgatc gaagccttgc gcggagaagc gcccctggga
     1801 aacgtgctcg aaatcggtac cggctgcgga taccaggcgg cactgctcgc gcggctcgca
     1861 acagaggtct attcggtcga gcgcattgcc ggattgctgg agaaggcgcg cggcaacctc
     1921 cgccctttgc gtctgtcaaa cctgaggttg gtccacgccg acgggacgca cggattgccc
     1981 gaggcagcgc ctttcgacgg catcatcgtc gccgcggcgg ctcccagagt tcccggtgcg
     2041 cttttgcagc agctggcgcc gggtggcaga atgatcgtgc ccgtgggaag cggcgatcag
     2101 gcgctgtgcc tcatcgagag ggctccgagc ggattcacgg aaaaatggct cgacgccgtg
     2161 cgtttcgtac ctttgagagg aggcaagcag tgaagctggc ctggattttt ctcgccgtcc
     2221 t
//
LOCUS       DASPZD010000043         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_335886, whole genome shotgun sequence.
ACCESSION   DASPZD010000043 DASPZD010000000
VERSION     DASPZD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335886"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VEM33_00805"
     CDS             <1..348
                     /locus_tag="VEM33_00805"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HYS50400.1"
                     /translation="IWRIWQHGDQEFLAQPGDKVYVFFRIFSPTRFSDQVQVRWYWKD
                     DARGWTLQDTIPIKILGGRAEGFRGYGFKSNYQPGDWKVQVETTDGREIGRVYFQLET
                     RPEWPRNFEIDLE"
     gene            complement(371..1204)
                     /locus_tag="VEM33_00810"
     CDS             complement(371..1204)
                     /locus_tag="VEM33_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50401.1"
                     /translation="MSRSHTASVASPRARLRALATALARLGDPVRRALRRLWAAPPAI
                     RVLAVAIAMLALWGAANWIVQTVRKPSEMFFPVGGALVKTPLETWRQYGSLFREHSTA
                     VISPELLAALAQIEGAGNPVARPRWSWRPSWNPFELYRPSSSAVGMFQITDAAFRDAK
                     RYCIHDHAVVVDGPWYDPTTCWFNGLYTRVVPSHAIEMTSALLDRRVANMLARSAATL
                     EQKQDLAAVIHLCGPARGQAYARRGFQPSGQRCGTHDLAGYLAQVNALKRLFARLAAL
                     D"
     gene            1250..1546
                     /locus_tag="VEM33_00815"
     CDS             1250..1546
                     /locus_tag="VEM33_00815"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HYS50402.1"
                     /translation="MHESDVSAEKLATDLRLVISDAEALLRATVGQAGETTAAARAKM
                     QETLESAKLKLGPLAEEAAEKARAADDYVRANPWQAVGIAALVGIALGLLMSRR"
     gene            1556..1939
                     /locus_tag="VEM33_00820"
     CDS             1556..1939
                     /locus_tag="VEM33_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYS50403.1"
                     /translation="MTEETGRAEGLFESLRNLAKTFLAVVQTRIEIFASEIDEERTRL
                     ARIAVLAAVAALCVALAVVLAVFFLVVLFWDTNRLLAIGVLAGVFAVGGVASCLGLRA
                     AISQRPKFLSATLAELRKDRTKLEG"
     gene            1939..2268
                     /locus_tag="VEM33_00825"
     CDS             1939..2268
                     /locus_tag="VEM33_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HYS50404.1"
                     /translation="MSALPQILERRARLLAEAEVQRRALSQGIAVCRNVLVVADRGIA
                     WATWLRARPYLVVAAATAIAVLRPKFALAWSARVLTLWRVGRLLYDVVKPALEDRARP
                     SHPDSQG"
     gene            complement(2259..>2722)
                     /locus_tag="VEM33_00830"
     CDS             complement(2259..>2722)
                     /locus_tag="VEM33_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50405.1"
                     /translation="LAAALAAKKRGVLIDVGHGGGSFDYTVAEPAIAQGFTPDTIGSD
                     IHAVSGNTPGMPYLPWVMSKFLNLGFSLEQVVAMATATPAKIIGKLDKLGTLAIGAPA
                     DVSILELVEGPVDFVDTRKNSRKGSRHLKPVATVRAGRPFGRPYPSPFAYP"
BASE COUNT          437 a          895 c          964 g          426 t
ORIGIN      
        1 atctggcgca tctggcagca cggcgatcag gagttcctcg ctcagccggg cgacaaggtg
       61 tacgtgttct tccgcatctt ctcgcccacg cgcttctccg accaggtgca ggtgcgctgg
      121 tactggaagg acgacgcccg cggctggacg ctgcaggaca cgattcccat caagatcctc
      181 ggcgggcgcg ccgaaggctt ccgcggctac ggcttcaagt cgaactacca gccgggcgac
      241 tggaaagtgc aggtcgagac caccgacggg cgcgaaatcg gccgcgtgta tttccaactc
      301 gaaacacgcc ccgagtggcc gcggaatttc gaaatcgacc tcgagtagcg ccagcccggg
      361 gcgcggaccc tcaatcgagc gccgccaggc gcgcgaaaag ccgcttcaac gcgttgacct
      421 gcgcgaggta gccggcgagg tcgtgggtgc cgcagcgctg ccccgagggc tggaagccgc
      481 ggcgcgcgta ggcctggccc cgcgcggggc cgcagaggtg gatgacggcg gcgagatcct
      541 gcttctgctc gagcgtcgcc gcgctgcgcg cgagcatgtt cgcgaccctg cggtcgagca
      601 gcgccgaggt catctcgatg gcgtggctgg gcacgacgcg cgtgtagagg ccgttgaacc
      661 agcacgtggt ggggtcgtac catggaccgt ccacgaccac ggcgtggtcg tggatgcagt
      721 agcgcttcgc gtcccggaac gccgcgtcgg tgatctggaa cattccgacc gcgctcgacg
      781 agggccggta cagctcgaag ggattccagc tcgggcgcca gctccagcgc ggacgcgcga
      841 cgggattgcc cgcgccttcg atctgggcga gcgcggcgag gagctcgggc gagatcacgg
      901 cggtggaatg ctcgcgaaag agcgatccgt attgacgcca ggtctccagg ggcgtcttca
      961 cgagggcgcc gcccaccggg aagaacatct cggacggctt gcgcaccgtc tggactatcc
     1021 agtttgccgc gccccagagc gccagcatcg cgatcgcaac cgcgaggacc cggatagcgg
     1081 gcggcgcggc ccacagcctg cgaagcgcgc gccgaacagg gtctccgagc cgggcgaggg
     1141 cggtggccag cgcgcgcagg cgcgcacgcg gcgaagcgac gctggcggta tgcgaacgtg
     1201 acatggggag atatcgtatc cggtaaaatc cccggcaaag gggccactta tgcacgaaag
     1261 cgacgtcagc gctgaaaaac tcgcaaccga tctccgcctg gtcatctcgg acgccgaggc
     1321 gctgttgcgt gcgaccgtgg gtcaggcggg agaaactacc gccgccgcgc gcgcgaagat
     1381 gcaggagact ctcgaatcgg cgaagctcaa gctcgggccg ctcgccgaag aggccgcgga
     1441 gaaggcgcgc gccgccgatg actacgtgcg cgcaaatccc tggcaggcgg tgggaatcgc
     1501 cgcgctggtc ggaatcgcgc tcgggctcct catgagccgc cgctaaggcc tcgccatgac
     1561 cgaagaaacc gggcgcgccg aaggcttgtt cgaatcgctg agaaatctcg cgaagacttt
     1621 cctcgccgta gtgcagaccc gcatcgagat cttcgcgagc gagatcgacg aggagagaac
     1681 gcggctcgcg cgaatcgcag tgctcgccgc ggtcgccgcg ctttgcgtcg cgcttgccgt
     1741 cgtcctcgcc gtgttcttcc tcgtggtgct gttctgggac acgaatcgtc tccttgcgat
     1801 cggcgtcctg gccggcgtct ttgccgtcgg cggagtggcc tcctgcctcg gcttgcgcgc
     1861 ggcgatctcg cagcggccca agttcctgtc cgcgaccctc gccgagctgc gcaaggaccg
     1921 gacgaagctc gaggggtgat gagcgctctt cctcagatcc tggaaaggcg cgcgcggctc
     1981 ctcgccgagg cggaagtgca gcggcgtgcg ctctcgcagg ggatcgccgt ctgccgcaac
     2041 gtgctcgtcg tcgccgaccg cggaatcgcg tgggcgacat ggctgcgtgc gaggccttac
     2101 cttgtcgtcg ccgccgccac cgccatcgcg gtgctgcgcc cgaagttcgc gctcgcctgg
     2161 agcgcgcgcg tgctcacgct gtggcgggtc ggacggcttc tgtacgacgt ggtgaagccg
     2221 gcgctcgagg accgcgccag gccgtcccat ccggattctc agggataagc gaacggcgac
     2281 ggataaggcc tgccgaacgg ccgcccggcg cgaaccgtgg cgaccggctt caggtggcgg
     2341 ctgcctttgc gcgagttctt gcgcgtgtcg acgaagtcca cggggccttc cacgagctcg
     2401 agaatggaaa cgtcggccgg agcgccgatc gccagcgtgc cgagcttgtc cagttttccg
     2461 atgatctttg ccggcgtcgc cgtggccatg gcgacgacct gctcgagcga gaaccccagg
     2521 ttgaggaatt tgctcatcac ccagggcaga tagggcatgc cgggggtgtt gccgctcacc
     2581 gcgtggatgt cgctgccgat cgtgtcgggc gtgaagccct gcgcgatcgc tggctccgcc
     2641 acggtgtagt cgaagctgcc gccgccgtgg cctacgtcga tcaggacgcc gcgcttcttc
     2701 gcggcgagcg ccgcggcgag ga
//
LOCUS       DASPZD010000044         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1058971, whole genome shotgun sequence.
ACCESSION   DASPZD010000044 DASPZD010000000
VERSION     DASPZD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058971"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="miaB"
                     /locus_tag="VEM33_00835"
     CDS             complement(<1..1196)
                     /gene="miaB"
                     /locus_tag="VEM33_00835"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HYS50406.1"
                     /translation="MTKKLFIRTFGCQMNEYDSDRMADLLRAAKGFEITDQPENADLI
                     LFNTCSVRERAQEKVFHDLGRVKHLKQARRDLLIGVGGCVASQEGAAIVARAPYVDLV
                     FGPQTLHRLPELIDARQRTGRPQVDVSFPEIEKFDRLPPARVEGPSAFVSIMEGCSKY
                     CSFCVVPYTRGEEVSRPFEDVLTEVADLAEQGVKEVTLLGQNVNAYRGRMEDGVIADL
                     ALLLEYVAEIPGIGRIRYTTSHPKEFTQRLIDAHARIEKLAPQVHLPVQSGSDRVLAA
                     MKRGYTALEYKSIVRRLRAARPGIGISSDFIVGFPGETERDFEATLKLVDEVGFDSSF
                     SFVYSRRPGTPAAELPDDTPQEAKRTRLARLQRNIDAHASRVSESLIGTHQRILVEGA
                     SRRDAG"
     gene            complement(1198..1533)
                     /locus_tag="VEM33_00840"
     CDS             complement(1198..1533)
                     /locus_tag="VEM33_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50407.1"
                     /translation="MARCRAACFALLPVLFGAVAVLAQTRPSPIPEDSRRGYIRHVEE
                     MAVTVDDKAMQLAAGATVRNQQNLIIVPMSIPREGAWAAYELDRDGQIFRVWLLTPDE
                     LARLRRRGG"
     gene            complement(1555..1631)
                     /locus_tag="VEM33_00845"
     tRNA            complement(1555..1631)
                     /locus_tag="VEM33_00845"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1595..1597),aa:Met,seq:cat)
     gene            1708..2892
                     /locus_tag="VEM33_00850"
     CDS             1708..2892
                     /locus_tag="VEM33_00850"
                     /inference="COORDINATES: protein motif:HMM:NF005788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HYS50408.1"
                     /translation="MDFDVVIIGGGLAGASLAAALAESRLRLALVERKAPSSPGPEWD
                     SRVYALTPASTAFLDRIGAWRHVDAARVTPIHDMRVFGDDGRSRLDFSAYESGTLQLA
                     ATVESSRLHHALWQGFECQRNLSLLCPAAPAELRRAGNRVEIGLVDGTVVTAKLAVGA
                     DGAESWARRAARIEARNRSYAQLGVVANFACARPHRNIAYQWFRGDGVLAYLPLPGRR
                     VSIVWSAPEARAADLLALAPAAFCARVSEAGDEALGALEPLTPPAAFPLSSMIADRMA
                     EARIALVGDAAHVLHPLAGQGVNLGLGDAHSLADLLRAAPDPGDRLLLRRYERARAED
                     ILALRWITDGLFRLFDADHRMVARIRNFGLNLTNANPVVKTLLARRAMGIGGGLHHGE
                     PS"
     gene            2889..>3587
                     /locus_tag="VEM33_00855"
     CDS             2889..>3587
                     /locus_tag="VEM33_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HYS50409.1"
                     /translation="MSLRFPALFLAAVFAATALADEASVRRGVEARFDGIKVDSVTKT
                     SYGELYEIVVGDTLFYTDEKVNFVFKGDIIDARSQKNVTEERQQKLAAIRFEDLPLDL
                     AIKQVRGNGKRVVAIFSDPFCPYCKNLDRALLRQDDITIYTFLYPILRAESPDKARTI
                     WCSPDRAKAYYDFMLNAREPAAAPSCSAPVDKWLALGKKIGVRATPTSFTTNGERILG
                     ARPEELVKLMGEGKK"
BASE COUNT          612 a         1134 c         1170 g          671 t
ORIGIN      
        1 tccccggcat ccctgcgcga ggcgccttcg acgagaatgc gctggtgcgt gccgatcagg
       61 ctttcgctga ccctgctcgc atgcgcatcg atattccgct gaagccgggc aagccgggtg
      121 cgcttcgcct cctgcggagt atcgtcggga agctccgctg caggggttcc cggccgccgg
      181 ctgtagacga acgagaacga gctgtcgaaa cccacttcgt ccaccagttt cagggtcgcc
      241 tcgaaatccc gctcagtctc cccggggaag cccacgatga aatccgagga gatgccgatg
      301 ccgggtctcg cggcgcgcag cctgcgcacg atcgatttgt attcgagggc ggtgtacccg
      361 cgcttcatcg ccgcgagcac gcggtcagaa ccggactgca ccgggaggtg cacctgggga
      421 gcgagctttt cgatccgcgc gtgcgcatcg atcagacgct gggtgaattc cttcggatgc
      481 gacgtggtgt aacgaatgcg cccgatgccg ggaatctccg cgacgtactc gaggaggagg
      541 gcaagatccg cgatgacgcc gtcttccatc ctgccgcggt aggcgttgac gttctgaccg
      601 agcagggtca cttccttgac gccctgctcg gcgaggtccg cgacctccgt gagcacatct
      661 tcgaaggggc gggacacttc ttcgccgcgg gtgtagggaa cgacgcagaa gctgcagtac
      721 ttgctgcagc cttccatgat cgatacgaag gcgctcggcc cttcgacgcg ggccggcgga
      781 agcctgtcga acttctcgat ttcggggaag gagacgtcga cctgaggccg gccggtgcgt
      841 tgccgagcgt cgatcaactc gggaagccgg tgcagcgtct gcgggccgaa cacgagatcg
      901 acatagggcg cgcgtgccac gatggccgcg ccttcctggc tcgcgacaca gccgcccacg
      961 ccgatgagga ggtcgcgccg tgcctgcttc aggtgtttca cgcggccgag atcgtgaaac
     1021 actttttcct gggccctttc gcgcaccgag caggtgttga acagtatcag gtccgcgttt
     1081 tcgggctggt cggtgatctc gaaacctttc gccgcgcgca gcaggtccgc catcctgtcc
     1141 gagtcgtact cgttcatctg gcagccgaag gtccgaatga agagcttttt cgtcatgcta
     1201 gccgccccgc cgcctcagcc gcgcgagctc atcgggtgtc agcagccaca cgcgaaaaat
     1261 ttgaccgtcg cgatccagct cgtaggcggc ccaggctccc tcgcgcggga tgctcatcgg
     1321 cacgatgatc aggttctgct ggtttcggac agtcgcgccg gcggcgagct gcatcgcctt
     1381 gtcgtccacg gtcacggcca tctcttcgac gtgccggatg tagcctcgcc tggagtcctc
     1441 cgggatgggg ctcggccgtg tctgagcgag gaccgctacg gccccaaaga gcaccggcag
     1501 gagcgcaaag catgcggcgc ggcatcgggc catgatcgct cgctagagga gaaatggtgg
     1561 cgaatcaggg actcgaaccc cggacctgcg gattatgatt ccgtcgctct aaccagctga
     1621 gctaattcgc cacgggaaaa ccggaaatta tcctttttgc gttcgatggt tgtcaaggaa
     1681 ggctctcttc acgggtacaa tcagcgaatg gatttcgacg tcgtcatcat cggcgggggg
     1741 cttgccgggg cgagcctggc ggcagcgctt gccgaaagca ggttaaggct tgcgctcgtc
     1801 gagcgcaagg cgccgtcttc gccgggcccc gagtgggact cgcgcgtcta cgcgctgact
     1861 ccggcgagca cggcgtttct cgatcgcatc ggtgcgtggc ggcacgtcga cgccgcgcgc
     1921 gtcacgccga ttcacgacat gcgggtcttc ggtgatgatg ggcgctcgcg cctggatttt
     1981 tccgcttacg aatcggggac gctgcaactc gcggcgacag tcgagtcgag ccggcttcac
     2041 catgcccttt ggcagggttt cgaatgccag cgaaatttat cgctgctgtg cccggcggcg
     2101 cctgcggagc tgcgtcgcgc cgggaatcgc gttgaaatcg gcctcgtaga cggcactgtg
     2161 gtcactgcga agctggcggt cggcgccgat ggagccgagt cttgggcgcg gcgcgccgcg
     2221 cgcatcgagg cgcgaaacag gagctacgcg cagctcggtg tcgtagccaa tttcgcctgc
     2281 gcgcggccgc atcgcaatat cgcgtatcaa tggtttcgcg gcgacggcgt gctcgcctat
     2341 ctccccttgc ccggccggcg cgtgtcgatc gtctggtccg caccggaagc gcgcgctgcc
     2401 gatctgctcg ccctcgcgcc cgcggcgttc tgcgctcgcg tgagcgaagc gggcgacgaa
     2461 gctctgggtg ccctggagcc gcttactccg ccggccgcct ttcccttgag cagcatgatt
     2521 gccgaccgga tggcagaggc aagaatagcg ctcgtcggcg acgcggcgca cgtcttgcac
     2581 ccgctcgccg ggcagggagt caatctggga ttgggtgacg cgcactcgct cgccgatctg
     2641 cttcgcgcag cccccgaccc gggagatcgc ctccttctgc gccgctatga gcgcgctcgt
     2701 gccgaggata tactcgcatt gcgctggatc accgacgggt tgttcaggct gttcgatgcg
     2761 gaccaccgaa tggtcgcgcg gatccggaac ttcggattga acctgacgaa cgccaatccg
     2821 gtcgtgaaga cgctgcttgc gcgccgcgcc atgggcatag gcgggggcct tcatcacggg
     2881 gaaccatcat gagtttgcgg ttcccggctc tatttctcgc cgcggtcttt gccgcaacgg
     2941 cactcgccga cgaggcctcg gtgaggcgag gcgtcgaggc ccgcttcgac ggcatcaagg
     3001 tggacagcgt gaccaagacc tcctatggcg aactgtatga gatcgtggtc ggcgacaccc
     3061 tgttctatac cgacgagaaa gtgaacttcg tgttcaaggg cgacatcatc gacgcccgct
     3121 cccagaagaa tgtcaccgaa gagcgccagc aaaaacttgc agcgatcagg ttcgaggatt
     3181 tgccgctcga tctcgcgatc aagcaggtgc gcggcaacgg caagcgcgta gtagccattt
     3241 tctccgatcc gttctgccct tattgcaaga acctcgaccg cgcgctgttg cggcaggacg
     3301 acatcacgat ctatacgttt ctctacccca ttctccgggc cgaatcgccg gacaaggcgc
     3361 gaaccatctg gtgctcgccg gaccgggcca aggcctacta cgatttcatg ttgaacgctc
     3421 gcgagccggc ggctgcgcct tcctgtagcg cgccggtcga taagtggctt gccctcggca
     3481 agaagatcgg cgtgagggcg acgccgacaa gcttcacgac gaatggggag agaatcctcg
     3541 gcgcccggcc cgaagagctg gtcaaactga tgggcgaagg caagaag
//
LOCUS       DASPZD010000045         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_698362, whole genome shotgun sequence.
ACCESSION   DASPZD010000045 DASPZD010000000
VERSION     DASPZD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698362"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VEM33_00860"
     CDS             complement(<1..393)
                     /locus_tag="VEM33_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HYS50410.1"
                     /translation="MKKLVLASSNPGKLREFEALLAPLGLEVVPQASLGVSDAEEPHG
                     TFVENALAKARHASHSTRLPALADDSGICVAALDGEPGVHSARFAGGPRSDARNNEKL
                     IKLLSDKDSRRAHYYCVVVFLRHAEDPEP"
     gene            complement(593..955)
                     /locus_tag="VEM33_00865"
     CDS             complement(593..955)
                     /locus_tag="VEM33_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HYS50411.1"
                     /translation="MALVHNVARLKPRRRELRRGLTIAEASLWKHLKDRSLRGRKFRR
                     QHSVGPYILDFYCPGERLAVELDGAAHDHEAAQERDAVRTRFLQSAGIRLVRFENREV
                     IENLEGVLAVIARRFMIA"
     gene            complement(1203..1922)
                     /gene="rph"
                     /locus_tag="VEM33_00870"
     CDS             complement(1203..1922)
                     /gene="rph"
                     /locus_tag="VEM33_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYS50412.1"
                     /translation="MRPSGRKPDELRPIAIDRHYTRHAEGSVLVAFGDTRVICTASVE
                     DGVPGFLRGRGQGWVTAEYGMLPRATHTRTDREAARGKQSGRTQEIQRLIARSLRAVT
                     DLEKLGECTVKLDCDVIQADGGTRTASVTGAFVALHDAVGTLLERGILAASPIRDFVA
                     AASVGLFEGVPVLDLDYAEDSECETDMNVVMTGSGGFVEVQGTAEGAPFSAAQMDALL
                     ALAKRGIAELIAKQKAALGIA"
     gene            complement(1919..2836)
                     /locus_tag="VEM33_00875"
     CDS             complement(1919..2836)
                     /locus_tag="VEM33_00875"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HYS50413.1"
                     /translation="MRFSIFQETRKGGREANEDRTSYAYTRDALLMVVADGMGGHLHG
                     EVASQIAVQLLTEAFQREAKPKLADPSGFLQKSITDAHFALGEYAKARNLIESPRTTC
                     VACVVQDSTAYWAHAGDSRLYHIRQGRIQAQTRDHSRVQMLVDQGRVREEAVAAHPDR
                     NKIFNCIGASALPQVELSKATTLREDDTLILCSDGLWGPLTGKLITGALVKSDLIKAI
                     PALLDLAELRAGADSDNISVVAMTWAETHPGAIAGEVSTKTLRRDGVTTQLEDFSKAD
                     HSDLTDAEIEQAIEEIRAAIRKHTPQKRS"
     gene            complement(2833..>3239)
                     /locus_tag="VEM33_00880"
     CDS             complement(2833..>3239)
                     /locus_tag="VEM33_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HYS50414.1"
                     /translation="RQTLAQEGPRLNPMYTPGFAPPEQYKNRDLLGPWSDCYAIGASI
                     FACLASAAPQAADNRAEKDRYVSAARVWDGKYSRQLLETIDWCLELDHLKRPQSVFAL
                     QKVLLREKDPEVHRKNSLIESVRGALMKRLGR"
BASE COUNT          522 a         1101 c         1043 g          573 t
ORIGIN      
        1 cggctcggga tcctccgcgt ggcgcaggaa aacgacgacg cagtagtagt gcgcgcgccg
       61 gctgtctttg tcggacagca atttgatcag cttttcgttg ttgcgcgcat ccgacctcgg
      121 cccgccggca aaccgcgccg agtgcacgcc gggctctcca tcgagcgccg ccacgcagat
      181 gcccgagtcg tcggcaagcg cgggaagccg cgtgctatgg ctcgcgtggc gcgccttggc
      241 gagcgcgttc tcgacgaaag tgccgtgcgg ctcctcggca tcggatactc cgaggctcgc
      301 ctgcggaacg acctccaggc cgagcggcgc gagcagcgcc tcgaactcac ggagcttccc
      361 gggattggag gaggcgagca cgagtttttt cacgcgtcca ttttacccgg tgaagcttca
      421 aacccgacca ccccgcccgc ttgcgcgggc acccctcctc aagagaggag gggaagatca
      481 gcatggcggc gttgctttcc cctcctcgaa tgaggagggg aagatcagca tggcggcgtt
      541 gctttcccct cctcgaatga ggaggggtgg cggccgaagg ccgccggggt ggtcaagcaa
      601 tcatgaatcg ccgcgcaatc accgccagaa ctccttcgag attctcgatg acttcacgat
      661 tctcgaatcg gaccagccgg attccagcgc tttgaaggaa ccgtgtgcgg acggcatcgc
      721 gctcttgcgc agcttcatgg tcgtgagccg cgccgtccag ctccacggcg agcctttctc
      781 ccggacagta gaagtccagg atgtaaggcc cgacgctgtg ctgacgtcgg aatttcctgc
      841 cccgcaggct cctgtctttg agatgcttcc acagactcgc ctctgcgatt gtgagtccgc
      901 ggcgaagttc ccggcgacgc ggtttcagtc gcgcaacatt atgaacaaga gccatgcacg
      961 gtgaaacgct cgtgcacgac gagagttgac gaacccagtg cgggattcaa acgcgaccac
     1021 cccgccgcct tcggcggcac ccctcctcaa gagaggaggg gaaatccgac ccccgccgcc
     1081 ttcggcggca cccctcctca agagaggagg ggaaatccga ccaccccgcc gccttcggcg
     1141 gcacccctcc tcaagagagg aggggaaatc agaagagccc gagcatcacg agagcccaat
     1201 cgtcaggcaa ttcccagcgc cgctttctgc ttggcgatga gctcggcgat tcccctcttc
     1261 gccagcgcaa gcagcgcatc catctgcgcc gcggagaagg gcgcgccttc ggcagttccc
     1321 tgcacctcga cgaagccgcc cgagcccgtc atcaccacgt tcatgtcggt ctcgcattcc
     1381 gaatcctcgg cgtagtccag atcgaggaca ggcacgccct cgaagaggcc gaccgacgcg
     1441 gcggcgacga aatcgcggat gggcgacgcg gcgaggattc cgcgctcgag cagcgtcccg
     1501 accgcgtcgt gcaaggcgac aaaagccccc gtgacgctcg cggtacgcgt ccctccgtcc
     1561 gcctggatca cgtcgcagtc gagcttcacg gtgcactcgc cgagcttctc caggtcggtc
     1621 accgcgcgca gggaccgggc gatcagccgc tggatttcct gcgtgcgccc ggattgcttc
     1681 ccgcgcgccg cttcgcggtc ggtacgcgtg tgagtcgcgc gcggcagcat accgtactcc
     1741 gcggtcaccc atccctggcc gcggccccgg agaaaccccg gcacgccgtc ctcgacgctc
     1801 gcggtgcaga tcacccgggt gtcgccgaac gcaaccagca ccgagccctc cgcgtgccgc
     1861 gtgtaatgcc ggtcgatggc gatgggacgc agttcgtcgg gcttgcgtcc ggagggcctc
     1921 atgagcgttt ttgcggcgta tgcttgcgga tcgcggcgcg aatctcctcg atggcctgct
     1981 cgatctccgc gtcggtcagg tcgctgtggt ccgccttgct gaaatcctcg agctgcgtgg
     2041 tcacgccgtc gcgcctcagc gtcttcgtcg acacttcgcc ggcaatcgcg cccgggtgcg
     2101 tttccgccca ggtcatcgcc acgacggaga tgttgtcgga atccgccccg gcccgcagtt
     2161 ccgcgagatc gagcagcgcg ggaatcgcct tgatgaggtc gctcttcacc agcgcccccg
     2221 tgatgagctt tcccgtgagc gggccccaca agccgtcgct gcacagaatc agggtgtcgt
     2281 cctcgcgcaa cgtagtggcc ttcgacagct ccacctgcgg gagcgcgctc gccccgatgc
     2341 agttgaagat cttgttgcga tccggatggg ccgcgacggc ttcctcgcgc acgcggccct
     2401 ggtcgacgag catctgcacg cgggaatgat cgcgcgtctg cgcctggatc cggccctgcc
     2461 ggatgtgata gagccgcgag tcgccggcgt gcgcccagta ggcggtcgaa tcctgcacga
     2521 cgcaggccac gcaggtggtc cgcggcgact cgatcaggtt gcgcgccttg gcgtattcgc
     2581 cgagcgcaaa gtgcgcgtcg gtgatgctct tttgcaggaa tccggacggg tcggcgagct
     2641 tgggcttcgc ctcgcgctgg aacgcttcgg tgaggagctg cacggctatc tgggaagcca
     2701 cctccccgtg cagatgtcct cccatgccgt cggcgaccac catgagcaag gcgtcgcggg
     2761 tgtaggcgta gctggtgcga tcctcgttcg cctcgcgccc tcccttgcgg gtttcctgaa
     2821 agatcgagaa tctcatcgtc cgagccgctt catcaaagcg ccgcgaacgc tctcgatgag
     2881 agagttcttg cggtgcactt cggggtcctt ttcgcgcagc agcaccttct gcagcgcgaa
     2941 cacgctctgc gggcgcttca ggtggtcgag ctccaggcac cagtcgatcg tttcgagcag
     3001 ctgccgggaa tacttgccgt cccacactct cgccgcggag acgtagcggt ccttctccgc
     3061 acggttgtcg gccgcctggg gcgccgcgct ggcaaggcag gcgaagatgc tcgcgccgat
     3121 ggcgtagcaa tcgctccagg gcccgagcag gtcgcggttc ttgtactgct ccggcggcgc
     3181 gaagccgggc gtatacatcg ggttcaggcg cgggccctcc tgggcgagcg tctggcggg
//
LOCUS       DASPZD010000046         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1543258, whole genome shotgun sequence.
ACCESSION   DASPZD010000046 DASPZD010000000
VERSION     DASPZD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543258"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEM33_00885"
     CDS             complement(<1..194)
                     /locus_tag="VEM33_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYS50415.1"
                     /translation="MIFCPTCGQENPAGARFCNNCTTPLEPAAAPAREERKVVTVLFA
                     DLVGFTARAEQLDPEDVRALL"
     gene            282..548
                     /locus_tag="VEM33_00890"
     CDS             282..548
                     /locus_tag="VEM33_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYS50416.1"
                     /translation="MVELLHKGPPVITDRQRQVIELIAAGYSNEEVARRLGISPRTAK
                     AHSDVLRQKLSCTRRRQIPSAYRSLTGRDPLSEGLGAVPAASGD"
     gene            729..3224
                     /locus_tag="VEM33_00895"
     CDS             729..3224
                     /locus_tag="VEM33_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYS50417.1"
                     /translation="MTRLLDETSARVIMLVAPAGYGKTTLARQWMTHRKHGWYSGNAA
                     SSDVAALALGLMATTQGLLPNAGRRLREWLPTSREPASEIGVIAQLIEQDLSEWPEEA
                     WLVLDDYHLVSSPASEELIERLFAKSSYRVLLTSRSRPTWAHARERLYGSVLELTESS
                     LAMSADEASAVLAGQDDQAVGGLVALARGWPAVIGLAALTPAPVRDELIPRALHDFLA
                     EEIFSALAPHIQSALTRLALLPRITRQVAQDTLGNDPEAILQTGVNAGILEFSSSSGC
                     FIHPLLRRFLEEKLVSDDASQLNAAVTSAAKALIRAGAWDDAFTLISRFSRPDLLDEI
                     LAGALITLVREGRVATVTEWVEFASVQGYESPYIDLADSELAFRNGRYERAEALARAA
                     TAGLPRDHALRSLGHFRAGQARNLMDDGRAALKHFKAAYETAQNSFDARNALWGEFTV
                     ASEIERPDAAALLDKFAAEGGRDGDAIVREACGRFALASLGGHLTDAIEGLASAGELI
                     ERVRDPLIRATYWRSYAAALVLHANYRRALEVSERALDEAGTFHLKFVRPHALVSSAA
                     AKVGLRQFASADREIEEIEQAAHEMRDQYLVVNARMLRCRSLLHQGAPEAALAATAEV
                     SPIGPSPGRRAELLATRSAAIACAGDPLTALEMVRPAESLTRGTEPRLLARWVAVVCE
                     AMLGRVEEREIERSFAETVSDGALDTLVFAYRLHPAILSTLGQNAAHNDYLSQLLAEA
                     NEQALARRYGLVREGVPRNREVLTPRETQVYELLAEGRSNSEIARALFISEKTAKVHV
                     RNVLHKLGVQNRTQAAVLAARDSASTFPAEVSP"
     gene            complement(3214..>4161)
                     /locus_tag="VEM33_00900"
     CDS             complement(3214..>4161)
                     /locus_tag="VEM33_00900"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYS50418.1"
                     /translation="TSVLAAVVVATLVEQYGDLLFGSVTAALRGMSLKDIGRAASALS
                     LTIPLYVPLTAVLVYVYREVPWSVVLFLIPAFTAQKLFLLYQEQRATSEELAEAIARQ
                     ERANLSFASALVATLDARDRYTAGHSAAVAIYARDIAERLGLNVEQQRLAHLAGLVHD
                     MGKIGLPPGLLEKAGPLTLSERRQMEQHSVIGERILLNVADYAEVARIVRHHHERMDG
                     RGYPDGLAGQDIPLLSRILAVADAYNAMTSDRPYREAMPSRVARLRMAQAVESQFDTT
                     VVAGFEALLAGAPEDYRAGSVFDIRSDSEIQISAVAATG"
BASE COUNT          817 a         1292 c         1291 g          761 t
ORIGIN      
        1 agtagcgcgc ggacgtcctc ggggtcgagc tgctcggcgc gggccgtgaa gccgacgagg
       61 tcggcgaaga gcaccgtgac gaccttgcgc tcctcgcgcg cgggcgcggc cgcaggctcg
      121 agcggcgtgg tgcagttgtt gcagaaacgc gcgccggccg ggttctcctg gccgcaggta
      181 ggacagaaga tcacgcgtca agcgtacgcc gaaaggcgta tcgatcaaga tacgccgcgc
      241 gatcaatagg cacgtgaagg caccgttgtt agcgtcgcgg catggtcgaa ctactgcaca
      301 agggcccgcc ggtcatcacg gatcggcagc gccaggtgat cgagctgatc gcagctggct
      361 actcgaacga ggaggtcgcc cgccgtctcg gcatctcgcc gcgcacggcg aaggcccact
      421 cggacgtgct acgccagaag ctcagctgca cccgacgccg gcagatcccg agcgcctacc
      481 gctcgctgac cggacgcgac ccgctctcag aaggcctcgg agcggtcccg gccgcctccg
      541 gggactgacg gcccgcgcct gcggcccttc ggggtcgcag gcgcggagtc ccttctcgac
      601 ctcttggcgc ggcgacgaat gagggttagc ctacgcccgc gggcttgcgg cgccccagag
      661 gcaggatggc agaagagacc caaatcgagg tcccactcgg gcgacgccac atcatcgaac
      721 gcccccgctt gacgcgcctg ctggacgaga cgtcggcgcg cgtaatcatg cttgtagccc
      781 cagctgggta tggcaagacg acgctggctc gccagtggat gacccaccgc aagcacgggt
      841 ggtactcagg caacgcggcg tcttcggacg tagcagccct agcacttggc ttgatggcta
      901 cgacccaagg gctgcttcct aatgcgggtc gccgcctgcg cgaatggcta ccaaccagcc
      961 gcgaaccggc aagtgagatt ggcgtcatcg cccaactcat cgagcaggac ttgagcgagt
     1021 ggcctgaaga agcctggctg gtacttgacg actaccacct cgtctcgtct ccggcctcag
     1081 aggaactcat agaaagactc tttgccaaaa gctcgtatcg cgtgttgctg actagtcgta
     1141 gccgcccaac gtgggcacac gcgcgcgagc gtctttacgg aagcgtcttg gagctgacag
     1201 agagttcgct cgctatgagt gccgacgaag ccagcgcagt cttggctggc caagatgacc
     1261 aggccgttgg cggactggta gctcttgcta gggggtggcc agctgtcata ggcctagctg
     1321 ctctgacgcc ggctccggtt cgtgacgaat tgatccccag ggccctccac gacttcctgg
     1381 cagaggagat cttctccgca ttggcacccc acatccagag tgccctgacc cgattggccc
     1441 tcttaccgag aatcactcgc caagtcgctc aagacacact cggaaatgac ccggaggcga
     1501 tccttcagac aggtgtcaac gcaggcatcc tcgagttctc gtccagcagt ggctgcttta
     1561 tccatcctct cttgcgccgg ttcctcgagg aaaagctcgt ttcggatgat gcgtcgcaac
     1621 tgaacgcagc cgtaacgtcg gcggcgaagg ctctcattcg ggctggggcg tgggacgatg
     1681 cctttacgct gatttcacgt ttctcccggc cggacctgtt ggacgaaatc ctcgcaggcg
     1741 cgctgatcac attagttcga gagggacggg ttgccactgt cacggagtgg gtggagttcg
     1801 cctcggtcca aggttacgaa tctccctaca tagatctagc tgactcggag ctggcgttcc
     1861 ggaacggtcg ctatgagcgc gccgaggcct tggcccgagc agcaacagct ggcctcccac
     1921 gagatcatgc cctccgttcg ctgggacact ttcgtgccgg ccaagcccgg aatttgatgg
     1981 acgatggtcg ggctgcgctc aaacacttca aagcggcata cgagactgcg cagaattcgt
     2041 tcgacgcccg aaacgccctg tggggagaat tcaccgtcgc gtccgagatc gagcggccag
     2101 atgctgccgc tttgctcgat aagttcgcgg cggaaggcgg gcgcgacggt gacgcgatcg
     2161 ttcgagaggc ctgtggccgg ttcgcactgg cctcgctcgg cggccacctt actgatgcaa
     2221 tcgagggcct ggcatctgct ggcgaactga tcgagcgggt ccgggatcct ctgatccggg
     2281 ccacgtactg gcgaagctat gcggcggcac tcgtcctcca cgccaactat cgccgtgcgt
     2341 tagaggtttc ggaacgcgcc ctggacgagg cagggacttt ccacctcaag ttcgttcggc
     2401 cgcacgcgct ggtcagcagc gctgctgcaa aggttgggct tcgccagttt gcatcagcgg
     2461 atcgagaaat tgaggagatc gaacaggcgg ctcatgagat gcgagaccag tatctggtgg
     2521 tcaacgctcg aatgctcaga tgccgatcgc tactccatca aggagcaccc gaagcggccc
     2581 tagcggccac ggccgaagta tcgccgattg ggccttcacc aggccgccgg gcggagttgc
     2641 ttgcgacgcg ctcagctgcc atagcgtgtg caggagatcc gctgaccgcg ttggagatgg
     2701 ttcgcccagc cgagagtctg accaggggca ctgaaccgcg gttgctcgct cgatgggtag
     2761 cagtcgtctg cgaggcaatg cttgggcgtg tcgaggaacg agagattgaa cgatcgtttg
     2821 cagagaccgt ctcagacgga gcgctcgata cccttgtctt cgcatatcgc cttcacccgg
     2881 cgatcctttc gaccctcggc cagaatgccg cccacaacga ctacctttct caactgttgg
     2941 ccgaggcgaa cgagcaagcc ctggcacgtc ggtacggttt ggtgcgcgag ggcgtgcctc
     3001 gaaaccgcga agtgctgacc ccgcgtgaaa ctcaggtata tgagcttctc gcagagggcc
     3061 gaagtaacag cgaaatcgct cgtgctctat tcatcagcga gaaaacagcc aaggtgcatg
     3121 tccgcaacgt tctccacaag ctcggcgtcc agaaccgcac gcaagcggcg gtccttgcgg
     3181 cccgtgatag cgcgtctacg tttcctgctg aagtcagccc gtagctgcga cggcgctaat
     3241 ttggatctca gaatcggagc gaatatcaaa cacgctgccg gcgcgataat cttccggagc
     3301 tccggctagg agggcttcaa accccgcgac gacagtcgta tcaaactggg actctacggc
     3361 ctgcgccata cgtaaccgag ctacacggct gggcatcgct tcacgatagg ggcggtcaga
     3421 agtcattgcg ttgtaagcat ccgcgacggc aagtatccgc gagagcaacg ggatgtcctg
     3481 ccctgccaaa ccgtccgggt accctctccc gtccatacgc tcgtgatgat gtctaacgat
     3541 ccttgcaact tcagcgtaat ccgcaacgtt caacaggatc cgctccccga tcaccgagtg
     3601 ttgttccatc tggcgacgct cgctgagggt gagcgggcca gccttttcca agagtccggg
     3661 aggcagaccg atctttccca tgtcgtgcac tagaccagca agatgggcta gcctctgttg
     3721 ttcgacgttt agccccaatc gctcggcaat atctcgtgcg tagatcgcga ccgccgcaga
     3781 gtggcctgcg gtgtatcgat cccgggcatc gagagtcgca accaatgcgc ttgcgaacga
     3841 aagattcgct ctctcctgac gggctatcgc ctcggccagc tcctccgacg tagctcgctg
     3901 ctcctgatag aggagaaaga gcttttgcgc ggtgaaggcc ggaatcagaa agaggacgac
     3961 gctccacgga acctcgcggt agacgtagac caagacagcc gtaaggggca cgtacagcgg
     4021 aatcgtcaac gaaagcgcgg aagctgcacg tccgatgtcc ttcagggaca tgcctcgtag
     4081 cgctgcggta accgatccga acaggagatc gccgtactgt tcaacgagag tggccacgac
     4141 aaccgccgcg agaacggacg t
//
LOCUS       DASPZD010000047         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_459073, whole genome shotgun sequence.
ACCESSION   DASPZD010000047 DASPZD010000000
VERSION     DASPZD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459073"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VEM33_00905"
     CDS             complement(<1..657)
                     /locus_tag="VEM33_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50419.1"
                     /translation="MTIVFRLAAALLAALAASGEAHSQTYPVRPIRFLVGSPPGSGND
                     QVSRLLGQMLAERLGQPVVVEQKPGAAGLLANEALTKSAPDGHTMVLLSGAHAASAVL
                     NRALPYDPVRDFGMVGTVVAYPLVISVAPGSPIRSFADLLERARAEPGKLTYSMTPGT
                     LVHLLGEWINIEAGTTILGVPYKGSANALVDVLAGRVDATIETGTASFAHVRSGRLRS
                     L"
     gene            757..2484
                     /locus_tag="VEM33_00910"
     CDS             757..2484
                     /locus_tag="VEM33_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYS50420.1"
                     /translation="MLTPALLLPRPEAGAACPGKFLIMFAASGSRSRWIANAFLICAL
                     VWFAGLEYRGLFMPDEGRYADIARSMLDSNDWVTPRLNGIKYFEKPPLQYWATAGTFA
                     LFGVDEWTARLWPALTGFLGIALTAFAGFRLAPNSPWLLTASAFAGCWGYFLGGQFLT
                     LDMGLTFFLTAAMLGFALSRRTDISSRAERGWMVLAWAAVACAVLSKGIVGIVIPGLA
                     LLVYLVIERDLSLLRRLHWVAGLCVFGAIVLPWFILVQQRNPEFFGFFFIREHFERYW
                     LPHHHRAGPWWYFIPILLVGLLPWTPSIPAALARAWRAPAQPGFRFDRFLVVWAGVVV
                     VFFSASHSKLPGYVLPAVPAILLLFARHYPALSERLRRAPAFVCVASGLALTLLAAGL
                     PTLSVLLSWTEFDAEYAVWLLAAGLALSAAGIAALRLLRRSRQEASLAVLGLGSLLAV
                     QLALSGTHVLDEHYSSERLIDTMVGEKHRFPREPPFYSVASFDQSVPFYLGRPVTLVG
                     YKDELAPGIAAEPWKFVSSVDEFVGLWQEHEQAFAIMTPPFYEKLRQGGLPGRVLARD
                     AWRVIVARR"
     gene            complement(2509..3222)
                     /locus_tag="VEM33_00915"
     CDS             complement(2509..3222)
                     /locus_tag="VEM33_00915"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HYS50421.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEEAGAAYIDVARTPQRGVAAMT
                     RFLEGKSGGRLPFAPPFLKAGNLVIAQTANILLWLAPRHGLAPKGEATRLWAHQLQFT
                     ISDWLVEVHDAHHPIGGAFYYEDQRPESKRRAAHFTAERLPKFLGYFERVLMRDKGGW
                     LLGKAFSYADLSLFQMIAGLRYAFPRAMQRLEAKVPRQVALHDRVASRPRIAAYLASK
                     RRIDFNRHDIFRHYPELDA"
     gene            complement(3244..>3835)
                     /locus_tag="VEM33_00920"
     CDS             complement(3244..>3835)
                     /locus_tag="VEM33_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50422.1"
                     /translation="RPLGFSPGQLQGLLCVSGIACCVAMSMPQVHMVAYCADLGFPAA
                     RGAEMLSLMMAFGIVSRLASGWIADHIGGLGTLLLGAVLQATALALFLPFDGLVALYV
                     VSALFGLFQGGIVPSYPLIVREYFSPREAGTRTGTVIMATLGGMALGGWMPGWIFDMT
                     GSYRAAFVNGIVWSLVTTGIALFLIYRSRRWIPRAA"
BASE COUNT          603 a         1245 c         1308 g          679 t
ORIGIN      
        1 gagcgagcgc agcctgcccg agcggacatg cgcgaaggag gccgtgccgg tttcgatggt
       61 tgcatcgacg cggcccgcca gcacgtctac caaggcgttc gcactgccct tgtaaggcac
      121 gccgaggatc gtcgtgcccg cctcgatgtt gatccactcg ccaagcaggt gcaccagcgt
      181 ccccggggtc atggagtagg tcagttttcc cggctcggcc cgcgcgcgct cgagcaggtc
      241 cgcgaacgag cggatcggcg agccgggcgc gaccgagatc acgagcggat aggcaaccac
      301 ggtgcccacc atgccgaagt cgcgcaccgg atcgtaaggc agcgcccggt tcagcactgc
      361 cgaagcggcg tgcgcgccgg agagcagcac catggtatgg ccgtcgggcg cggacttcgt
      421 cagcgcttcg ttggcgagaa ggccggctgc gcccggcttc tgctcgacga cgaccggctg
      481 gcccaagcgc tcggcgagca tctgcccgag caggcggctg acctggtcgt ttccagaacc
      541 cggcggcgag ccgaccagga aacggatcgg gcggaccgga taggtttgcg aatgggcctc
      601 ccccgaggcg gcaagcgccg cgagcagcgc ggcggcgagt cgaaatacaa tggtcatttt
      661 cgggaacagg cttttgggga gggagcagcg attgtatagc gagcctgaca gtgatctgaa
      721 agctcgacgt cagcgccccg caaggcagag cggtccatgc tgacgcccgc cctgcttcta
      781 cctcggcccg aggccggcgc agcctgcccc ggaaaattcc tcatcatgtt cgccgcttcc
      841 ggctcacggt cccgatggat cgcgaacgct ttcctgatct gcgccttggt atggtttgca
      901 ggtctcgagt accgcggact cttcatgccc gacgaagggc gttacgccga tattgcccgt
      961 tcgatgctgg actcgaacga ctgggtgacg ccgcggctca acgggatcaa gtatttcgaa
     1021 aagcctccgc tgcagtactg ggcgaccgca ggcacgttcg ccctgttcgg cgtggacgag
     1081 tggacggccc gtctgtggcc ggccctgacc ggttttctcg ggatcgccct cacggcgttc
     1141 gcggggtttc gcctcgcgcc gaactcgccg tggctcctga ccgcgtcggc gtttgccggt
     1201 tgctggggat acttcctcgg cggccagttc ctgacgctcg acatggggct caccttcttt
     1261 ctgaccgcgg cgatgctggg ctttgccctg agccggcgca cggacatttc ctcgagggcg
     1321 gagcggggct ggatggtgct tgcatgggca gcggtggcgt gcgccgtctt gagcaaggga
     1381 atcgtgggaa tcgtgattcc gggcctggcg ctgctcgtgt acctcgtgat cgagcgtgac
     1441 ctgtcgcttc tccggcggct gcattgggtc gcgggcttgt gcgtgttcgg cgcgatcgtg
     1501 ctgccctggt tcatcctggt tcagcagagg aacccggaat ttttcggctt ctttttcatc
     1561 cgcgagcact tcgagcgcta ctggttgcct catcatcacc gggccggacc gtggtggtac
     1621 ttcatcccga ttctgcttgt cgggctctta ccctggacgc ccagcatccc cgccgccctg
     1681 gcaagagcgt ggagggcacc cgcccagccg ggattcaggt tcgaccgctt tctcgtcgtc
     1741 tgggccggcg tggtggtcgt ctttttcagc gcgtcgcact cgaagctgcc cgggtacgtc
     1801 ctgccggcgg ttccggcgat tcttctgctg ttcgcacgac actatccggc cctgtccgaa
     1861 cgcctgcgcc gcgccccggc cttcgtctgt gtcgcgagcg gcctcgcctt gacgctcctt
     1921 gccgctgggc ttccgactct gagcgtgctc ctttcgtgga cggaattcga cgccgagtat
     1981 gcggtctggc ttctcgcggc cggactggca ttgtcggcgg caggtatcgc cgcgctccgg
     2041 ctcctgcggc gctcgcggca ggaagcctcc ctcgcggttc tcgggctggg cagcctgctc
     2101 gccgtccagc tggcgctttc gggcacgcat gttctcgacg agcactactc ttcggagcgg
     2161 ctcatcgaca ccatggtcgg cgaaaaacac cggtttcccc gggaaccgcc cttctacagc
     2221 gtggcgagct tcgatcagag cgtgcccttc tatctcggcc gtcccgtgac cctcgtcggg
     2281 tacaaggacg agctggcacc gggaatcgcg gcggagccct ggaagttcgt cagctcggtc
     2341 gatgaatttg tcgggctctg gcaggagcac gagcaggcct tcgcgatcat gactccgcct
     2401 ttctatgaga agctcaggca gggggggctt ccggggcgcg tgctcgcgcg agacgcgtgg
     2461 cgggtgatcg tcgccagacg atagcttccg gaccgctgct ccggatcgct aagcatcgag
     2521 ttccggatag tgcctgaaga tgtcgtgccg gttgaaatcg atgcgccgct tcgacgcgag
     2581 gtaggcggcg atgcgcggcc gcgacgcgac gcggtcgtgc agcgcgactt gacgcgggac
     2641 tttcgcctcc agcctctgca tcgcgcgggg gaaggcatag cgcaggcccg cgatcatctg
     2701 gaagagcgac aggtccgcgt aggagaacgc cttgccgagg agccatccgc ctttatcgcg
     2761 catcagtacc cgctcgaagt agccgaggaa tttcggcagg cgctcggcgg tgaagtgcgc
     2821 ggcgcggcgc ttcgattcgg gcctctggtc ctcgtagtag aaggcgcccc cgatcgggtg
     2881 gtgcgcatcg tgcacttcga ccagccagtc ggagatggtg aactggagct ggtgcgccca
     2941 gagccgcgtc gcctcgcctt tcggcgcaag gccgtggcgc ggggcgagcc acagaagaat
     3001 gttcgccgtc tgcgcgatca ccagatttcc cgctttcagg aaaggcggag cgaacggcag
     3061 gcgtccgccg gatttgcctt ccaggaagcg cgtcatggcc gcaacgccgc gctgcggcgt
     3121 ccgcgcgaca tcgatgtagg cggcgcccgc ttcctccagc gcgagccgca cgaattctcc
     3181 gcggccctgt atcgagggcc agtaatagag ctcgtagcgc atggggtcct ccttgaggac
     3241 aggtcaggct gcgcgaggga tccagcgccg cgagcgataa atgagaaaga gcgcgatccc
     3301 ggtcgtcacc aggctccaga cgatgccgtt gacgaacgcc gcacggtagg agccggtcat
     3361 gtcgaaaatc cagccgggca tccagccgcc gagcgccatg ccgccgagcg tcgccatgat
     3421 cacggtgccg gtgcgcgtgc ccgcttcgcg cggggagaag tactcgcgca cgatgagggg
     3481 gtaggagggc acgatgcctc cctgaaacag gccgaacagg gccgagacca cgtagagcgc
     3541 gaccaggccg tcgaagggca ggaacagcgc gagcgcggtc gcctggagca cggcgccgag
     3601 caacagtgtg cccaggcctc cgatgtggtc ggcgatccag cccgaggcga gccggctgac
     3661 gatgccgaaa gccatcatga gcgagagcat ctccgcgccg cgcgcggcgg gaaagcccag
     3721 atccgcgcag tacgcgacca tgtgcacctg cggcatggac atcgcgacgc agcacgcgat
     3781 ccctgacacg cacagcagac cctgcagctg gccgggggag aacccgagcg gccgc
//
LOCUS       DASPZD010000048         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1423484, whole genome shotgun sequence.
ACCESSION   DASPZD010000048 DASPZD010000000
VERSION     DASPZD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423484"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(444..1847)
                     /locus_tag="VEM33_00925"
     CDS             complement(444..1847)
                     /locus_tag="VEM33_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013160158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HYS50423.1"
                     /translation="MKRYGRPDRCVRAALRSPGRPQEALRENCRQFWMAIASGRSSED
                     AGVDAGVSPAVGVRWFRRAGGMPPTHFSQSSKPLSGCRLSFTEREEIAILRAQGIGVR
                     AIARLLDRPPCTISRELRRNAARRHGAPEYRATTAQWHADRSARRPKPAKLAVNPILQ
                     DYVQDRLAGMIAKPDGAPLVGPKVVWKGRRAVHRQHRRWATAWSPEQISRRLRLDFPE
                     DETMRISHEAIYQALYVQGRGALRRELSACLRTGRALRMPRVRTRKPGRAFVSPEIMI
                     SQRPAEAADRAVPGHWEGDLIIGLESSAIGTLVERTTRFTMLLHLPRMTGHDQEVRLK
                     KGPALAGHGAEAVRDAITRAISTMPEQLRRSLTWDQGAELAQHARLKIDAGMQVYFCD
                     PHSPWQRGTNENTNGLLRQYFPKGTDLSAHNADDLEAVALALNTRPRKTLGWKTPAET
                     LDEFLRVSHTRSVATIG"
BASE COUNT          375 a          670 c          659 g          416 t
ORIGIN      
        1 cgagcgtgac tcgagcggac ggcgaagtcg tgtggtcctg acgccgcggt gctggcgtca
       61 agttcgcggg aagcaaaact tctcgcggat gacggtggca agaaaagccg ttcaccgggg
      121 agagctcgaa gtaagccgta agccatcgcg cagggaaggc cgggatgcct ccgctgaacc
      181 tgtatgctcg tgtgcgtttt tttgtgcatg tttgcacacg agaccgcggg tgcagcgcgc
      241 acccggcttt ccctgcgccc tcttgttgtt atcgagggcg gcaatcgacg caaagctcgg
      301 gcgcatcgcg ccgcgagatc gcgaggctgt gtctgcagac acgcgctgtt tgaaaattga
      361 atcagcagat atttatcgcg cacgcatgcg ccacatcccc cacgtggtca tcgcccggct
      421 cgaccgggcg acccagggcg gattcaaccg atcgtcgcaa cacttcgtgt atggcttact
      481 cgtaggaatt cgtcgagggt ttccgccggt gttttccaac caagagtttt cctgggcctg
      541 gtgttgagtg caagggctac ggcctcaagg tcatcggcgt tgtgagcgct gagatcggtg
      601 ccctttggaa agtactggcg tagcaacccg ttggtgttct cgttggtacc gcgctgccaa
      661 gggctgtgag gatcgcagaa gtagacctgc atgcccgcgt cgatcttcag gcgtgcgtgc
      721 tgagccagct cggctccttg gtcccaggtc agcgaccgac gcagttgctc aggcatggtt
      781 gagatcgcgc gggtgatggc gtcgcgcact gcttcggcac catgtccggc gagtgcgggt
      841 cctttcttca agcgaacttc ctggtcatgg cccgtcatgc ggggcaggtg cagcagcatg
      901 gtgaaacgtg tcgtgcgctc caccaaggtg ccaatcgcgg aactttcaag acctatgatg
      961 aggtcccctt cccagtggcc cggcacggct cgatcggctg cctctgccgg acgttggctg
     1021 atcatgatct cgggagaaac gaaggccctg cctggcttgc gcgtgcgcac cctcggcatc
     1081 cgcagcgccc gcccggtgcg caagcaggcc gatagttcac ggcgcaacgc tcctcgacct
     1141 tgcacataaa gtgcctgata gatcgcctcg tgactgatgc gcatcgtctc atcctcggga
     1201 aagtcgagcc gaaggcgacg agaaatctgt tccgggctcc acgccgtcgc ccagcgtcgg
     1261 tgctgtcgat gcacggcccg gcgccccttc cacacgacct tcgggccgac gaggggcgcc
     1321 ccatccggct tggcgatcat accggcaagc ctgtcttgca cataatcttg caggattgga
     1381 ttaaccgcca acttcgccgg ctttggccgt cgagcggacc gatcggcgtg ccattgtgcc
     1441 gtggtcgctc gatattctgg agcgccgtgg cggcgcgccg cattgcgccg gagctcacga
     1501 gagatcgtgc atggagggcg atcgagcagc cgagcgatgg ctcgcacacc aatcccctgt
     1561 gcgcgcaaga tggcgatctc ctcacgttcg gtgaacgaaa ggcggcaccc tgatagaggt
     1621 tttgacgact gcgagaaatg cgtcgggggc atgccacccg ccctccgaaa ccaccgtacg
     1681 ccgacagccg gcgacacccc ggcgtcaacc ccagcatcct cgctcgagcg ccccgaggca
     1741 atcgccatcc agaactgtct acaattctct cgcagggcct cttgaggccg gcctggcgat
     1801 cgcaaagctg ctcgcacaca ccgatccgga cgcccgtatc tcttcatcgc aacctccttc
     1861 gccgaagtgt tacgacgacc ggttgaatcc gcccagggtc gcccggtcaa gccgggcgat
     1921 gacagcaata tatgtagcgg cggtagtact tcactccccc gcctgcagct tgcggggaag
     1981 cggaaacagc tcaggcgtat cgctcttgac gatgcgcggg acctcggcgg gcggctcgct
     2041 cttgctgaag tccagttcct cgatccgtcc cgcccgcttg tcgatcttgt cggcggagat
     2101 caggatctgc cggacatcct
//
LOCUS       DASPZD010000049         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_214280, whole genome shotgun sequence.
ACCESSION   DASPZD010000049 DASPZD010000000
VERSION     DASPZD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214280"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VEM33_00930"
     CDS             <1..1044
                     /locus_tag="VEM33_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYS50424.1"
                     /translation="MDLQAPPGDARLFIAERGGKIRVVDGGVLLTDPFLDISARVLPF
                     VGEDGLLSFAFDPQYTTKGFVYVHFIEKNLNANGDIVVERYQVSPTNPNVLQTPGTPV
                     IRIPHHEATNHYGGHVFFGPDGMLYLSTGDGGGGDNQFGHAQDAASLLGKMLRIDVNA
                     LNPNNTPPYAIPSGNPVWPNVGRNEVWAVGLRNPFRYAFDFPTSQLYIADVGQNVREE
                     IDVVDASLPGLNYGWSVMEGLRCFPNGGQCNMAGLTLPVYDYDHSQGNAIIGGYVYRG
                     AAIPELRGTYLFSDLHSGFLRGLTLQNGVATVVQAPNANAGTDVTLSFGQDGMGEVYI
                     LTAANRVLKVVRP"
     gene            complement(1209..2264)
                     /locus_tag="VEM33_00935"
     CDS             complement(1209..2264)
                     /locus_tag="VEM33_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYS50425.1"
                     /translation="MFFKQLFDPGSSTLTYLVADDASHEAVLIDPVTEQVERDVRLLR
                     EHGLALKYTLETHVHADHVTAAHALKQATGAQTAVCRDCNAQGYDRLLGDGDVILFGH
                     EEILTIATPGHTPGSVSYLWRDRVFTGDTLLIGGCGRTDFQNGSAEALWTSITEKLFA
                     LDEQILVYPAHDYKGRRVSSIGEEKRFNARVGGKTRAEFLSIMSNLNLPMPARIHEAV
                     PANLEGGTGQPAVASAIGRPKVAVQSVSAKQLADALRAPGVHLLDVRTPEEFNALRIS
                     GSINVPLAALDPAALRESMDANEPVYVVCQTGTRSQFAATELRAAGFSRVVHVDGGTN
                     AWTVAGLPVVRGGPVRA"
     gene            complement(2296..3354)
                     /gene="mnmH"
                     /locus_tag="VEM33_00940"
     CDS             complement(2296..3354)
                     /gene="mnmH"
                     /locus_tag="VEM33_00940"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HYS50426.1"
                     /translation="MELKRLDAATVAQLDAFDEVIDVRSPAEFALDHVPAAVNCPVLD
                     DAERAHVGTLYKQISPFDAKKIGAALVAKNIARHIDEHFRGRGQGWRPLVYCWRGGQR
                     SAAMAYVLRQVGWDAATLEGGYRAYRREVITQLDRMPQGFRFQVVCGATGSGKSRLLA
                     ALAEQGAQVLDLECLARHRGSLLGELPGEPQPSQKMFDSLVWCALKRVDPARPVFVEA
                     ESKKIGQLQVPAQLLTRMREGECLRIEVPLPERVRFLIEEYRHFLEKPVALKEKLQCL
                     TSRYGRAVIERWLAQTDSGAWDELVADLLVTHYDPSYLRAAAQNYLHYDEAQALSLAR
                     LDPIEIAHAAGELLKAEP"
     gene            3369..>3526
                     /locus_tag="VEM33_00945"
     CDS             3369..>3526
                     /locus_tag="VEM33_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYS50427.1"
                     /translation="MNAPADLIRLTQFSHGGGCGCKIAPAVLSEILAKTPIRGLPKDL
                     LVGIESSD"
BASE COUNT          578 a         1188 c         1129 g          631 t
ORIGIN      
        1 atggatctgc aggcaccgcc cggcgatgcg cggcttttca tcgccgagcg cggggggaaa
       61 atacgtgtcg tcgatggagg tgtcttactg acggatccct ttctcgacat atccgcccgc
      121 gttcttccgt tcgttggcga agacggactg ctctcgttcg ccttcgatcc gcagtacacc
      181 acgaagggct ttgtctacgt ccacttcatc gagaagaacc tcaacgccaa cggcgatatc
      241 gtagtcgagc gctaccaggt atcgcccacc aaccccaacg tgctccaaac tccggggaca
      301 ccggtgatcc ggattcctca tcacgaggcg accaaccact acggcggcca cgtgttcttc
      361 ggtccggatg gcatgcttta tctgtcgacc ggcgacggtg ggggagggga caaccaattc
      421 ggccatgccc aggatgcggc gtcgctcctc ggcaagatgt tgcgcatcga tgtcaacgcg
      481 ctcaatccga acaacacccc tccctacgcc atcccaagcg gcaatcccgt atggccgaac
      541 gtaggtcgga atgaagtctg ggcggtcgga ctgcgcaatc cctttcgcta cgcgttcgat
      601 ttcccgacca gccagctgta catcgccgac gtcggacaga acgtgcgaga ggaaatcgat
      661 gtggtcgacg cgagcctccc gggcctcaac tacggctgga gcgtcatgga gggcttgcgg
      721 tgctttccga acggcggcca atgcaatatg gcggggctga ctctgccggt ttacgactac
      781 gaccacagcc agggcaacgc gatcatcggg ggctacgtct atcgcggcgc cgcgatcccc
      841 gagctgcggg ggacctacct cttttccgac ctgcacagcg gattcctgcg cggtctgacg
      901 ttgcagaatg gcgtcgccac cgtcgtgcag gcgccgaacg cgaacgcggg aaccgacgtc
      961 actctgtctt tcggtcagga cggcatgggc gaggtctata tcctcaccgc ggccaaccgc
     1021 gtgctgaagg tcgtgagacc ctgaggcggt ttcctattcc gcttctcccc gcttcaagct
     1081 gccgaccacg ctccacccgg cgtcgatcac cgtcatcttt accgagtctt ccggggcggg
     1141 ttcgccgcgc cacgcgcggc gccggtctcg agcattccga tctccgcgca cgcgttcagg
     1201 aacggttttc aagcacggac cggaccgccc cgcactacgg gcaatcccgc caccgtccag
     1261 gcgttggtgc cgccgtccac gtgcacgacg cggctgaaac ccgccgcgcg cagctccgtc
     1321 gcggcgaact ggctgcgcgt tccggtctgg caaacgacat ataccggttc gttcgcgtcc
     1381 atgctctcgc gcagcgccgc gggatcgagc gcggcgagcg ggacgttgat cgagccggaa
     1441 attctaagcg cgttgaactc ctcgggcgtg cgcacgtcca ggagatggac tccgggcgcg
     1501 cgcagcgcgt ccgcgagctg tttcgcggac acgctctgga ccgcgacctt cggccgcccg
     1561 atggcgctcg caaccgcggg ctggcccgtg ccgccttcga ggtttgccgg gaccgcctcg
     1621 tggatgcgcg caggcatggg caggttgagg ttgctcatga tggacaggaa ctccgcgcgg
     1681 gtcttgccgc cgacgcgcgc gttgaagcgc ttttcctcgc cgatgctgct caccctgcgc
     1741 cccttgtagt cgtgggccgg gtacacgagg atctgctcgt cgagcgcgaa gagcttctcc
     1801 gtgatgctcg tccacagcgc ctccgcgctc ccgttctgga agtccgtgcg tccacagccg
     1861 ccgataagca gggtgtcgcc ggtgaagacc cggtcgcgcc acaggtacga gacgcttccc
     1921 ggcgtatgtc cgggggtggc gatcgtgaga atctcctcgt gcccgaaaag gatcacgtcg
     1981 ccgtccccga gcaggcggtc gtagccctgc gcattgcagt ccctgcagac cgcggtctgc
     2041 gcgccggtgg cctgcttgag cgcgtgcgcg gcggtcacat ggtcggcgtg cacatgggtc
     2101 tcgagcgtgt acttgagcgc gaggccgtgc tcgcgcagga ggcgcacgtc gcgctcgacc
     2161 tgctcggtca ccgggtcgat cagcaccgcc tcgtggctcg cgtcgtcggc gacgagatag
     2221 gtgagagtgg acgagcccgg gtcgaagagc tgtttgaaga acatggcatt ctccgcgttc
     2281 ggcgaatccg ccggctcaag gttcggcctt cagcaactcc ccagcggcgt gcgcaatctc
     2341 gatcgggtcg agccgggcta gggacagcgc ctgagcttca tcataatgca aatagttttg
     2401 cgcggccgcg cgaagatagg acgggtcgta gtgggtgacg agcaggtccg cgaccagctc
     2461 gtcccacgcg cccgaatcgg tctgcgcaag ccagcgctcg atcaccgcgc ggccgtagcg
     2521 gctcgtcagg cattgcagct tctccttgag cgctacgggc ttctccagga aatggcggta
     2581 ttcctcgatc aggaagcgca cgcgctcggg caggggcacc tcgatgcgca ggcactcgcc
     2641 ctcgcgcatg cgggttagga gctgcgcagg cacctgcagc tggccgattt tcttgctttc
     2701 cgcctcgacg aagaccggac gcgcgggatc gacacgcttc agcgcgcacc ataccaggct
     2761 gtcgaacatc ttctgcgagg gctgcggctc gcccggaagc tccccgagca gcgagccgcg
     2821 atggcgggcg aggcactcca gatccagcac ctgcgcgccc tgttctgcga gcgccgcgag
     2881 gaggcgactc ttgccgcttc cggtggcgcc gcacaccacc tggaaccgga aaccttgcgg
     2941 catccgatcg agctgcgtaa tgacttcgcg acggtacgcc cggtagccac cttcgagggt
     3001 cgccgcgtcc cagccgacct ggcgcagcac gtaggccatc gccgcggagc gttgccctcc
     3061 gcgccagcag tagacgaggg gacgccagcc ctgcccgcgt ccgcggaaat gctcatcgat
     3121 gtgccgcgcg atgttcttcg ccacgagcgc ggcgccgatc ttttttgcgt cgaaagggga
     3181 gatctgcttg tacagcgttc ccacgtgggc gcgctcggca tcgtccagca ccgggcagtt
     3241 cacggctgcc ggcacgtggt cgagcgcgaa ttcggcgggc gagcgcacgt cgatcacctc
     3301 gtcaaaggca tcgagctgtg ctaccgtggc ggcgtccagg cgtttcagct ccatccctaa
     3361 ttttatccat gaacgcgccc gccgatctca tccgcctgac gcagttctcc cacgggggcg
     3421 gctgcggctg caagatcgcg ccagcggtcc tctccgagat cctcgccaag accccgatcc
     3481 gcgggctgcc gaaggacctg ctcgtgggga tcgaatcgag cgacga
//
LOCUS       DASPZD010000050         4604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_333680, whole genome shotgun sequence.
ACCESSION   DASPZD010000050 DASPZD010000000
VERSION     DASPZD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333680"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..721
                     /gene="fabG"
                     /locus_tag="VEM33_00950"
     CDS             2..721
                     /gene="fabG"
                     /locus_tag="VEM33_00950"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HYS50428.1"
                     /translation="MIRALVTGGSGSIGAAACRRLAADGRHVYVHANSRLARAEKLAA
                     EITASGGSAHAVSFDIADRDATGTALQKLLVDGPIQIVVNNAGVHDDAVMPGMRPEQW
                     SRVIDVSLNGFFNVTQPLLMPMIGTRWGRIVSVSSVAALAGNRGQTNYAAAKAGLHGA
                     TKALALELASRGITVNAVAPGIIESAMTESVFSRDAIEKFVPMKRAGTPEEVAELIGF
                     LASERAAYISGQIISINGAMI"
     gene            complement(734..1564)
                     /locus_tag="VEM33_00955"
     CDS             complement(734..1564)
                     /locus_tag="VEM33_00955"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HYS50429.1"
                     /translation="MQSVFRICKGLLLLWLAASFAHADQLPLWEAGAGFAVTTLPHYR
                     GSDQAKTWVLPVPYVVYRGASLKVDDDRRLRGLLGDKVEVELSLSGSPPAKDNDARRG
                     MPDLDATLELGPSLNITLFRSENRNEKLELRLPVRTVFSSDFSHVRQTGWVFQPNLSL
                     DFKNVLGDRGWKLGLQGSLIYTNRRYNQYFYAVDPVFVTADRPAFDPSGGYGGLNFLA
                     AVSKHFPGFWVGGFAKWDSVSGAVFADSPLVRARSNFSGGFAVAWIFRASTAKVEAEN
                     "
     gene            complement(1624..1710)
                     /locus_tag="VEM33_00960"
     tRNA            complement(1624..1710)
                     /locus_tag="VEM33_00960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1674..1676),aa:Leu,seq:cag)
     gene            1778..3988
                     /gene="rnr"
                     /locus_tag="VEM33_00965"
     CDS             1778..3988
                     /gene="rnr"
                     /locus_tag="VEM33_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HYS50430.1"
                     /translation="MKKRDIARATDPHREREASRYEHPISSREFILRTLAESGVPLTD
                     EELAQRLAINPKERDAFAKRLGAMEREGQILRNRKGAILVASKLDLVTGRVEGHPDGY
                     GFVIAEEGEDLYLSPHEMRKVLHGDRVMVRESGVDRRGRREGTIVEVVERVNARVVGR
                     LHGERGFLFVQPAERRISQTILIAPEDAGNARVGQVVVAELTMQPDGRTQPIGRVVEV
                     LGEAADPGIEIEIALRKHELPFKFSAQAEALAAKFPPVPRPDDVAGREDLRSLELVTI
                     DGETARDFDDAVYCEKTSEGFRLVVAIADVSHYVREGDALDVAARERGNSVYFPRRVI
                     PMLPEQLSNGLCSLNAEVDRLCMACDMAIGAKGKIGAYRFYPAVMRSRARLTYTSVAA
                     ALADPRSEAAQKLKGLLPRLQLLKALFGALLKAREERGAIDFETIETQFVFDGEGRLA
                     RIEKVERNDAHRIIEECMLAANVCASDILSKRKHPALYRVHEGPTPEKLQSLREFLKG
                     FGLALDGGDKPTAKHYAKLLETVRKRPDVQLLQTVLLRSLQQAQYSPENVGHFGLAYE
                     HYTHFTSPIRRYPDLLVHRAIKAVLAGGRYNPGNWAQLGSHCSQTERRADEATREVAA
                     WLKCYYMRDRVGEEFEGSVSAVTSFGIFVALDEFYVEGLVHISELGQDYFQFDATRHQ
                     LIGDRTRKRFRLADRVKVRIVRADLETSRIDFVLAGDSAGGQRHVRSLRRRARS"
     gene            4000..>4604
                     /gene="rlmB"
                     /locus_tag="VEM33_00970"
     CDS             4000..>4604
                     /gene="rlmB"
                     /locus_tag="VEM33_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HYS50431.1"
                     /translation="MKPTRAIFGFHAVLARLRVDAASVTDIYLDEDRKDARAQDLVLA
                     AEKAGVSLIRVPTKRLDGFYGGGRHQGVVAMAFEKPARESLEDLLDSVKEAPLLLVLD
                     GVTDPHNLGACLRVADAAGAHAVIAPKDRAAGISTTVSRVASGAAESVPYYMVTNLAR
                     TLDELKERNVWIIGADERAEKTLYESDLPAATAWVLGAEGEG"
BASE COUNT          841 a         1477 c         1528 g          758 t
ORIGIN      
        1 cgtgatacgg gcgctcgtca ccggcggcag cggcagcatc ggcgctgcgg catgcaggcg
       61 cctcgcggcc gacggccggc atgtttacgt ccacgcgaac agccgcctcg cacgggcgga
      121 gaagcttgcc gccgagatca ccgcaagcgg gggctccgcg cacgccgtgt ccttcgatat
      181 cgccgaccgc gacgccaccg ggacggcgct gcaaaaattg ctcgtcgacg gccccatcca
      241 gatcgtggtc aacaatgccg gagttcacga cgacgcggtg atgccgggaa tgcggccgga
      301 gcaatggtcc cgggtgatcg atgtctccct caacggattc ttcaacgtca cgcagcctct
      361 gctcatgccg atgataggga cgcggtgggg aaggatagtc agcgtttctt ccgtggctgc
      421 gcttgcgggc aaccggggcc agaccaacta cgctgccgcc aaggcgggcc tgcacggcgc
      481 gaccaaggcg ctcgccctgg aactggcgag ccgcggcatc acggtcaatg cggtcgcgcc
      541 cgggatcatc gaatccgcca tgacggagag cgttttttcc cgagacgcga tcgagaagtt
      601 cgtgccgatg aagcgcgccg ggactccgga ggaggtcgcc gagctgatcg ggtttctcgc
      661 ctccgaacgc gccgcgtata tttccgggca gatcatctcg atcaacggcg cgatgatctg
      721 aatccgcctg cagctagttc tccgcttcga ccttggcggt cgaagcgcga aaaatccagg
      781 cgacggcaaa gcccccgctg aaattacttc tcgccctcac cagcgggctg tcggcgaata
      841 ccgcgccgct gacgctgtcc catttcgcaa acccgccgac ccagaaaccg gggaagtgct
      901 tgctgaccgc cgcgaggaag ttcagtccgc cgtagccgcc gctcgggtcg aaggcgggcc
      961 ggtccgcggt gacgaacacc gggtcgaccg cgtagaaata ctggttgtag cgccggttcg
     1021 tgtagatgag gctcccctgc aggcctagct tccatccgcg gtctcccagg acgttcttga
     1081 aatccaggct caagttcggc tgaaaaaccc agccggtttg cctcacgtgg gagaaatccg
     1141 acgagaagac ggtgcgcacc ggcagtcgca attccagttt ttcatttctg ttctcggatc
     1201 gaaagagggt gatgttgagc gagggaccca attccagtgt ggcatcgaga tcgggcatgc
     1261 cgcgccgcgc atcgttgtct ttggcgggcg gggagccgct caggctgagc tcgacttcga
     1321 ccttgtcgcc caacaggccg cgcagcctgc ggtcgtcgtc gactttaagg gacgctcccc
     1381 ggtagaccac ataaggcacc gggagcaccc aggtcttggc ttgatccgag ccgcgatagt
     1441 gaggaagggt cgtaactgcg aatcctgcgc cggcctccca cagcgggagc tgatccgcgt
     1501 gtgcgaagct cgccgccagc cacagcaata gaaggccctt acagatccgg aaaacggatt
     1561 gcacgtcgag cggatttgag ttcgggcttt gaaaaaccgg ttagaatctt gttggagcga
     1621 agctggtgcc gaggaaggga ctcgaacccc cacagtgttg ccaccgctag gacctgaacc
     1681 tagtgcgtct accaattccg ccacctcggc gaaagggccg cgccagttcc ggtcttcagc
     1741 ccgagcgcgg attctatagg aaagcaccga tttgtcaatg aaaaagcgcg acatcgcgcg
     1801 cgcaaccgac cctcatcgcg aacgcgaagc gtcgcgctac gaacatccga tctcgagccg
     1861 cgagttcatt ctgcggacgc tcgcggaaag cggcgtgccg ctcaccgacg aggagctcgc
     1921 gcagcgcctc gcgatcaatc cgaaggagcg cgatgcgttc gcgaagcgtc tgggcgcgat
     1981 ggaacgcgag ggccagatcc tgcgcaatcg caagggcgca atcctggtcg cctccaagct
     2041 cgatctcgtc accggacgcg tcgagggcca tcccgacggc tacggcttcg tgatagccga
     2101 ggagggcgag gatctgtacc tcagcccgca cgagatgcga aaggtcctcc acggcgatcg
     2161 cgtgatggtg cgcgaatcgg gcgtggacag gcgcgggcgc agggaaggga cgatcgtcga
     2221 ggtagtcgaa cgcgtcaacg cccgagtggt gggacgcctg cacggcgagc gcggcttcct
     2281 cttcgttcag cccgcggagc ggcgcatcag ccagaccatc cttatcgcgc cggaagatgc
     2341 gggcaatgcg cgggtgggtc aggtcgtcgt cgccgaattg actatgcagc ccgacggccg
     2401 cacgcagccg atcggccgtg tagtcgaggt gctgggagag gccgccgatc ccgggatcga
     2461 gatcgagatc gcgctcagga aacacgaatt gccgttcaag ttctcggcgc aggccgaggc
     2521 gctcgctgcg aagtttccgc ccgtgccgcg ccccgacgac gtcgcgggcc gggaggactt
     2581 gcgatcgctc gagctcgtga ccatcgacgg cgaaacggcg cgggatttcg acgacgccgt
     2641 gtactgcgaa aagacaagcg aagggtttcg gctcgtggtc gccatcgcgg acgtgagcca
     2701 ctacgtgcgc gaaggcgacg cgctcgacgt ggcggcgcgc gagcgcggca actcggttta
     2761 ctttccccgc cgggtcatcc cgatgctgcc tgagcagctc tccaacggcc tgtgctcgct
     2821 gaacgccgaa gtcgaccgct tgtgcatggc gtgtgacatg gcgatcggcg cgaagggcaa
     2881 gatcggcgcg tatcgcttct atcccgcggt catgcgctca cgcgcgaggc tcacgtatac
     2941 ctcggtggcc gcggcgcttg ccgacccgcg gagcgaggcg gcgcagaagc tgaagggatt
     3001 gctgccgcgt ttgcagttgt tgaaggctct cttcggagcg ctcctcaagg cgcgggagga
     3061 gcgcggcgcg atcgacttcg agacgatcga aacccagttc gttttcgacg gagaggggcg
     3121 gcttgcgcgc atcgagaagg tggagcgcaa cgacgcccac cgcatcatcg aggagtgcat
     3181 gctcgccgcg aacgtctgcg cctcggacat tctctccaag cgcaagcacc cggcgctgta
     3241 ccgcgtccac gaaggcccga ctccggagaa gctgcagagc ctgcgcgagt ttctcaaggg
     3301 attcggcctc gcgctcgacg gcggggacaa gcccaccgcc aaacactacg cgaagctgct
     3361 cgagaccgtg cgcaagcgtc cggacgtgca gctgctccag acggtgctgc tgcgctcgct
     3421 gcagcaggcg cagtacagtc cggagaacgt cggccacttc gggctcgcct acgagcacta
     3481 cacgcacttc acttctccga tccgacgcta cccggacctc ctcgttcatc gcgccatcaa
     3541 ggcggttctc gccggcgggc gctacaaccc cggcaactgg gcgcagctgg ggagccactg
     3601 ctcgcagacc gagcgccgcg cggacgaggc cacgcgcgag gtcgccgctt ggctcaagtg
     3661 ctactacatg cgcgaccgcg tcggcgagga gttcgaaggc tcggtctcgg cggtgacgag
     3721 tttcggcatc ttcgtggcgc tcgacgagtt ctacgtggag ggactggtgc acatctccga
     3781 gctcgggcag gactatttcc aattcgacgc cacccgccat caactgatcg gcgaccgcac
     3841 ccggaagcgg ttccgtctgg ccgacagagt gaaggtcagg atcgtccggg cggatctcga
     3901 gacgagccgc atcgattttg tgctcgcggg agattccgcg ggcggccagc gtcacgtccg
     3961 atcccttcgc cggcgcgcaa gatcctagga cggctcagga tgaagccgac ccgcgccatt
     4021 ttcggattcc acgccgtgct cgctcggctg cgtgtcgatg cggcgtcggt cacggatatc
     4081 tatctcgacg aagaccgcaa ggacgcgcgg gcgcaagacc tcgtgctcgc cgcagaaaaa
     4141 gccggcgttt ccctcattcg cgttccgact aagcgcctgg acggtttcta cggcggcggc
     4201 cgccaccagg gcgtggtggc gatggcgttc gaaaagccgg cgcgggaaag cctggaggac
     4261 ctgctcgatt cggtgaagga agcgcccctt ctgctggttc tcgacggcgt gaccgatccg
     4321 cacaatctcg gggcctgttt gcgggtcgcg gacgccgccg gcgcccacgc ggtgatcgca
     4381 ccgaaggacc gtgccgccgg catcagcacg accgtgtcca gggtggcgag cggcgcggcc
     4441 gaatccgtgc cttactacat ggtgacgaac ctcgcccgca cgctcgacga actgaaggag
     4501 cgcaacgtct ggatcatcgg cgcggacgag cgcgccgaaa agacgctcta cgagtcggat
     4561 ctgcctgcag ccaccgcctg ggtgctgggc gcggaaggcg aggg
//
LOCUS       DASPZD010000051         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1659716, whole genome shotgun sequence.
ACCESSION   DASPZD010000051 DASPZD010000000
VERSION     DASPZD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659716"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..759)
                     /locus_tag="VEM33_00975"
     CDS             complement(145..759)
                     /locus_tag="VEM33_00975"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HYS50432.1"
                     /translation="MELLSKRPEWKSADIDWRMVRRWLRAGAFSAVVAAGLASPRPVQ
                     ADAQADEYRVKAAFLYKFGSYIEWPSGSFASADSPVTIGVMGAEALAEELAQIVAGRN
                     VNGRPVRVRRLRPGDPIAGLHVLFVGRADGGRLAEILAAARGQALLTVTESDEGLELG
                     SMINFVVVEDKVRFDIAPPPSESSNVKISARLLGVARKVVSKSS"
     gene            complement(747..2630)
                     /locus_tag="VEM33_00980"
     CDS             complement(747..2630)
                     /locus_tag="VEM33_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYS50433.1"
                     /translation="MLARVRWRLALVFNVLFLASAGVGAQLMRVADLADMSIEELGNI
                     QITSVSKHTERLSDAPAAIFVITGDDIHRSGATRLAEALRLAPNLEVARVSASSYAIS
                     ARGFNNTIANKLLVLIDGRTVYTPLFSGVFWDAQDVMLEDVERIEVISGPGATLWGAN
                     AVNGVINVITRRASDTQGAFAYGQAGSLDRGAGARYGGAMGAAGAYRVYARFFDVFNT
                     SRTNGASASDGWGKGQVGFRTDWGTATNALTLQGDAYRGSLDQAIGDDSSISGNNLLG
                     RWDRDLAEWGQLQIQSYFEHTERNIPGTFAEHLNLFDVEFQHGLRAIGAHRLTWGGGY
                     RYGDDRVSNSAFLAFLPANRGMRWANVFAQDEIALLESLRVTLGAKFESNYYTGTEPL
                     PSARLAWKPQPQSLVWGAVSRALRAPSRIDRDFFVTAGPTQLAGGPDFASEVVKVIEL
                     GYRDQPSPQATYSVSLFHNIYDRLRSVEPVSPTTSVLGNKMEGTADGLEAWGNYQAAR
                     NWRLSAGVFFLRQHLRPKPDSGDPNVSAAGNDPAHQWLLRSSFDLPNSTELDIRIRSV
                     GALPNPSVPAYTAADIRIAWRLQRELELSVVFIDSGHVEFGNPATASEMSSGAYIKLK
                     WNY"
     gene            complement(2776..3411)
                     /locus_tag="VEM33_00985"
     CDS             complement(2776..3411)
                     /locus_tag="VEM33_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYS50434.1"
                     /translation="MTVTVLLADDHAVVRDGLRALLETGDLQVVAVAGNGREAVAEAL
                     RLRPDIVIMDIAMPELDGVEATRRIVEKCPETRVLILSMYLSAEHIYRALQAGAQGYV
                     LKESAGEEVVEAIRALRAGKRYLSHRITETVIDDYLREGANVSPLDSLSLRERDVLQL
                     VVEGRTNAAIAQALSLSPKTVETYRARIMKKLKVRDTVELVKFSMRHGLIS"
     gene            complement(3413..>3683)
                     /locus_tag="VEM33_00990"
     CDS             complement(3413..>3683)
                     /locus_tag="VEM33_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS50435.1"
                     /translation="EALNNVAKHSNAKRVEIAIRRANGLATLSVRDNGIGFDPKRIER
                     SNREAGWGLLIMRERAEAVGAQFSLKTGLRTGVQVLVEYHVQSGV"
BASE COUNT          628 a         1226 c         1183 g          646 t
ORIGIN      
        1 tggtcatgtc cgacacgagg acccggtggt aggccctcaa gtcgtaaccg attatcgccc
       61 ccagagcgac gatcaaagac accagcgtga tgacgagcac catcccgagg agcttttgcc
      121 gcaacgagcg cggagagggc agcttcacga cgatttcgac acgactttgc gcgcgactcc
      181 gaggaggcgc gcgctgattt tcacgttgct cgattcggaa gggggaggcg caatgtcgaa
      241 acggaccttg tcctcgacca ccacgaaatt gatcatgctg ccgagctcga gtccctcgtc
      301 cgactcggtg accgtgagca aagcctgacc tcgggccgca gccaggattt cggccaggcg
      361 gccgccgtcg gcccggccga cgaaaagaac gtgcagccct gcgatcgggt cgcccggccg
      421 gagcctgcgc acgcgcaccg gccggccgtt gacgttgcgg cccgcgacga tctgcgcgag
      481 ctcctcggcc agcgcctcgg cgcccatcac gccgatcgtc accggactgt ccgcgctggc
      541 gaagctcccg ctcggccatt cgatgtagct gccgaacttg tagaggaacg ccgccttgac
      601 acgatactca tcggcctgcg cgtcggcttg caccgggcgc gggcttgcga gccccgctgc
      661 gaccaccgcg gagaaagcac cggcgcgcag ccatcgtctc accatccgcc aatcgatatc
      721 ggcggatttc cattcgggtc gtttgctcag tagttccatt tcagctttat gtacgccccg
      781 ctcgacattt cgctggcggt agccgggttc ccgaattcca cgtggccgga atcgatgaag
      841 acgaccgaaa gctcgagctc gcgctgcagc cgccacgcaa tgcggatgtc cgccgcggta
      901 taagccggga cacttgggtt gggcagcgcg ccgacgctgc ggattcggat gtcgagctcc
      961 gtgctgttgg gaagatcgaa cgacgagcgc aggagccact ggtgcgccgg gtcgttgccc
     1021 gcggcgctca cgttgggatc gccgctgtca ggcttcgggc gcaggtgctg cctgaggaag
     1081 aacactccag cgctcaagcg ccagtttctc gccgcctgat aattgcccca ggcttcgagc
     1141 ccgtcggcgg tgccttccat cttgttgccg agaacggaag tggtcgggga aacgggttca
     1201 acgctgcgca gcctgtcgta gatgttgtgg aacagggaga cggaatacgt cgcctgcggc
     1261 gagggctggt cgcggtagcc gagctcgatg accttgacca cctcggacgc gaagtccggg
     1321 ccgcccgcga gctgcgtggg accggcggtc acgaaaaagt cgcgatcgat gcgcgaaggc
     1381 gcgcgcaggg cgcgcgatac cgcgccccag accaggctct gcggctgggg tttccaggcg
     1441 agcctcgccg acggcagggg ctcggtgccg gtgtagtagt tgctctcgaa cttggccccg
     1501 agcgtgaccc gcagactctc cagcagcgcg atctcgtctt gcgcaaacac gtttgcccag
     1561 cgcatgccgc gattggcggg caggaaggcc agaaaggcgc tgttgctgac gcggtcgtct
     1621 ccgtaacggt agccgccgcc ccacgtcagt ctgtgggcgc cgatcgcacg caggccgtgc
     1681 tggaattcga catcgaaaag gttcaggtgc tccgcgaaag tccccgggat attgcgctcg
     1741 gtgtgctcga aataggattg aatttgcagc tgtccccatt cggcaaggtc gcggtcccag
     1801 cggccgagca ggttgtttcc ggagatgctc gaatcgtcgc cgatcgcctg gtcgagcgag
     1861 ccccggtacg cgtcgccctg cagggtcaac gcattcgtcg cggtccccca gtcggtgcga
     1921 aagccgacct gccccttgcc ccagccgtcg ctcgcgctgg cgccgttcgt tcttgaggtg
     1981 ttgaacacgt cgaaaaacct ggcgtagacg cggtacgctc cggcggcacc catcgcgccg
     2041 ccgtagcgcg cgccggcgcc ccggtcgagg ctgcccgcct gcccgtacgc gaatgcgccc
     2101 tgggtatcgg aggcgcgccg cgtgatcacg ttgatcacgc cgttgacggc gtttgcgccc
     2161 cacaaggtcg ctccggggcc gctgatcacc tcgatccgct cgacgtcttc cagcatcacg
     2221 tcctgtgcat cccagaagac gccggagaaa agcggcgtgt acacggtgcg gccgtcgatc
     2281 aagaccagca gcttgttcgc gatggtgttg ttgaaaccgc gcgcgctgat ggcgtacgag
     2341 ctcgcgctca cgcgcgcgac ctcgaggttg ggcgcgagcc tcagcgcctc ggcaaggcgg
     2401 gtcgcccccg agcggtggat gtcgtcgccc gtgatcacga aaattgctgc gggcgcatcc
     2461 gagagccgct cggtgtgctt cgacaccgag gtgatctgga tgttgccgag ttcctcgatg
     2521 ctcatatcgg cgaggtccgc gacacgcatg agctgcgcgc cgacgcccgc cgaggcaagg
     2581 aagaggacat tgaagacgag cgcgagccgc cagcgcaccc tcgccagcat cgagcaagtg
     2641 cttgaaattc cactgcggaa caccctccgg ccccgtattg ctgcttcgac gattcgagcc
     2701 taagcagacc gcagggcggc gtccatcggg gaatcccgac acatcgtgtc agggaaaccc
     2761 gataaacgaa aaaaatcagc tgattagtcc atgtctcatc gagaacttca cgagctccac
     2821 ggtatcgcgg accttgagct tcttcatgat gcgcgcgcgg taggtctcca cggtcttcgg
     2881 cgaaagcgac agcgcttgcg cgatcgcggc gttggtgcgc ccctcgacga ccagctgcag
     2941 cacatccctc tcgcgcagcg agaggctgtc gagcgggctg acgttcgcgc cttcgcgaag
     3001 gtagtcgtcg atcaccgtct cggtgatccg gtggctcaag tagcgcttgc cggcgcgcag
     3061 cgcgcggatc gcctccacca cttcctcgcc cgcggattcc ttcaggacgt acccttgagc
     3121 gcccgcttgc aaggcgcggt agatgtgctc cgcgctcagg tacatggaga ggatcagcac
     3181 gcgcgtctcc ggacatttct cgacgatgcg ccgcgtcgcc tcaacgccgt cgagctcggg
     3241 catggcgatg tccatgatga cgatgtccgg gcgcaggcgc agcgcctcgg cgaccgcctc
     3301 gcggccgttg cccgcgactg caaccacctg caggtcgccc gtttcgagca aggcccgaag
     3361 accgtcccga acgaccgcgt gatcgtcggc gagcagcacc gttaccgtca tttcacaccc
     3421 cgctctggac gtggtactcc acgagcacct gcacgcccgt acgcaggccc gtcttcagcg
     3481 agaactgggc cccgacggct tcggcccgct cgcgcatgat caacagtccc cagccggctt
     3541 cccggttgga tctctcgatt cgcttcggat cgaagccgat gccattgtcg cggaccgaca
     3601 gcgtcgccaa accgttggcg cgtcggatcg cgatctctac gcgtttagcg ttcgagtgct
     3661 tggcgacgtt gttgagcgcc tcc
//
LOCUS       DASPZD010000052         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1445129, whole genome shotgun sequence.
ACCESSION   DASPZD010000052 DASPZD010000000
VERSION     DASPZD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445129"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VEM33_00995"
     CDS             complement(<1..1235)
                     /locus_tag="VEM33_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYS50436.1"
                     /translation="MANSINHIRTIALVGQAGSGKTTLAEALLAKAGAVHAAGSVERG
                     TTVCDYSPGEKQLRHSLKLAVASFDAKVDDEQIRVHLLDTPGYPDFLGHALPALAGVE
                     TAAIVINAQNGIEMMTGRFMQYAQKRGLDRLIIVNKIDAPNVDCERLLERIQQTFGKE
                     CLPLNLPAGNCGKVSDTFFAPSGEADFSSVEEAHRRLIEQVVEVDEKLMETYLEKGEV
                     TPEELHEPLEQALREGHLIPVVFTSAKTGAGIAELLEVIVKLLANPTEGNPPVYFSTP
                     REAGSGKGEALTAVPDADKHVLAHVFKIETDPYIGRLAVFRVHQGRLTPNMQLYIGDG
                     RKPIKPTHLYMLRGKTQTEVQEAIPGDICAIARIEEIQFDNILHDSPEDAHLHARPLE
                     LPTSVFGLAVMPKKRQDEQ"
     gene            1623..>2348
                     /locus_tag="VEM33_01000"
     CDS             1623..>2348
                     /locus_tag="VEM33_01000"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYS50437.1"
                     /translation="MNDLFDQLQDTFGSNYTMKSELRAGGMARVFVARDETLGRDVVV
                     KVLPPELTYNFSAARFAREIKLAAALQEPHIVPVLTAGQTVGGLPYFTMPFVRGESLR
                     TRILKGPVPFEDSLSILHDVARALAYAHGQGIVHRDIKAANIFITTRGQAKVLDFGLA
                     KVTPRAEAADRDSTAMTELTTAGSTLGTVAYMSPEQALGKELDARTDLFSFGVVLYEM
                     ATGVLPFRGDTSAAIAHALLGTDP"
BASE COUNT          402 a          756 c          729 g          461 t
ORIGIN      
        1 ttctgctcgt cctggcgctt cttcggcatc accgcgagac cgaagacgga agtcggcagc
       61 tcgagcgggc gcgcgtgcag gtgagcgtcc tcgggcgaat cgtgcaggat gttgtcgaac
      121 tggatctcct cgatcctggc gatggcgcaa atgtcgcccg gaatggcctc ctgcacttcc
      181 gtctgcgttt ttccgcgcag catgtagagg tgcgtaggct tgatgggctt gcggccatcg
      241 ccgatgtaga gctgcatgtt cggggtcaag cgcccctggt gtacgcggaa taccgcgagg
      301 cggccgatat acggatccgt ctcgatcttg aagacgtgcg cgaggacgtg cttgtcggca
      361 tcgggaaccg ccgttaacgc ttcccccttc ccgcttcccg cttcccgcgg agtgctgaag
      421 tacacgggcg gattcccctc ggtcggattc gccagcagct tgacgatcac ctcgagcagc
      481 tcggcgatgc cggcaccggt cttcgcggaa gtgaacacca cgggaatcaa atgcccctcg
      541 cgcagcgctt gctcgagggg ctcgtgtaat tcttcgggcg tgacctcgcc cttttcgagg
      601 taggtctcca tcagcttctc atcgacttcg accacctgct cgatgagccg gcggtgcgcc
      661 tcctccaccg acgagaagtc ggcctcgccg gagggtgcga agaaggtgtc ggagaccttg
      721 ccgcaattgc ccgccggaag attcagcggg aggcattcct tgccgaacgt ctgctggatg
      781 cgctcgagca gacgctcgca atcgacgttc ggtgcgtcga tcttgttgac gatgatcagt
      841 cggtcgagtc cgcgcttctg cgcgtattgc atgaagcggc cggtcatcat ctcgatcccg
      901 ttctgcgcgt tgatgacgat cgccgcggtc tcgaccccag cgagcgcggg aagcgcgtgg
      961 ccgaggaagt ccgggtagcc cggcgtgtcg aggaggtgga cgcggatctg ctcgtcgtcg
     1021 acctttgcat cgaagctcgc gaccgcaagc ttgagggagt gtcgaagctg cttttcgccc
     1081 ggtgagtagt cgcagacggt agtgccccgc tccacgctcc cggcggcgtg caccgctccc
     1141 gctttcgcga gcaacgcctc ggccagggta gtcttgccgc tgcccgcctg cccgacgagc
     1201 gcgatcgtcc tgatgtggtt aatggaattc gccacgggcc tgccctcctt caatttccta
     1261 ttgtatagca ggaagatcgg ggaactgcaa actgcgaact gcaaactgac gactaccggc
     1321 caagggctga tggctggtcg gcgggtgctg cgggcgggct gtttggtgcg cgcgccccgg
     1381 cgcaggggtt tgcgatcgtt gacctcaaac catttgaata gtgccccaca tgaatggtgt
     1441 cccacagaag aacagaccgc acgtagccca aagccgaccc gccgaaagcc cccggccggt
     1501 agtcgtcagt tgcagtttgc agttcccagg ccggtagtca tgcgttgccc cctgcgcagc
     1561 tcgcttcccg cctcccgctt cccgcttccc gctttacatt agactgcccg cgcgaggaac
     1621 ccttgaacga tctcttcgat cagctccagg acacgttcgg cagcaactat accatgaaga
     1681 gtgagctgcg cgcgggtggg atggcccgcg tgttcgtcgc tcgcgacgaa accctcggcc
     1741 gcgacgtcgt cgtcaaggtg ctgccaccag agctcaccta caatttctcg gcggcacgat
     1801 tcgcccggga gatcaagctc gccgcggcgc tccaggagcc acatatcgta ccggtgctca
     1861 ccgccggaca aacggtaggc gggctcccat actttacgat gccgttcgtg cgcggggagt
     1921 cactgcgcac ccgcatcctg aaaggtcccg tcccgttcga ggacagcctg tcgatactgc
     1981 acgacgtcgc ccgcgctctc gcctacgcac acgggcaggg catcgttcac cgcgatatca
     2041 aagcggccaa cattttcatt accacgcgcg gccaggcgaa ggtcctcgat ttcggacttg
     2101 caaaagtcac cccgagagcc gaagcggctg accgcgattc taccgccatg acggagttga
     2161 ccacggcggg cagcaccctg ggcacggtgg cttatatgtc gcccgagcag gcactcggca
     2221 aagaactgga cgcgcgcacc gacctgttct ccttcggcgt cgtactgtac gagatggcga
     2281 cgggtgtgct gccgtttagg ggcgacacat ctgcggcgat cgctcatgcg ctgctcggca
     2341 cagatcct
//
LOCUS       DASPZD010000053         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_696393, whole genome shotgun sequence.
ACCESSION   DASPZD010000053 DASPZD010000000
VERSION     DASPZD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696393"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VEM33_01005"
     CDS             complement(<1..752)
                     /locus_tag="VEM33_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYS50438.1"
                     /translation="MSEIARPGPDESAIDGEALKKAEQFIEAEEGASNKLRGALGIFV
                     SATAVAMSLFHLYSAYAIVPTQVLRPVHVGFVLFLSYLLFPVAKRYRHRIMWWDWAAA
                     FAAVAVIAYILQGGDDIWDRNTSPDNWDLVFGVALILLILEGMRRASGWVMPIVVTFF
                     VLYALFGEQLPGSWAHRSYEVGRLVGHLYMTLEGIFGVAVDVSSSLIILFTIYGAFLQ
                     FSGAGKFFIDFSFSAMGGKSTSAGRTVVLASFL"
     gene            complement(837..1994)
                     /locus_tag="VEM33_01010"
     CDS             complement(837..1994)
                     /locus_tag="VEM33_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50439.1"
                     /translation="MLLKAPWVGGSLIVLAGCAAAPLAPLPAHLLWQDQAFEYDAALV
                     SVGKRDLFQLDAGLLSKLRDPRIQNSSAQYRLNHLVTLLFEPQTKDFSYSRNHSTIAA
                     ETWRRKSGDCLSLTVLSYSLAKALDMSVQMQEVRVPVVFDRRGSVEFLNRHVNLLISG
                     RSVGGLYLKDGLLRAGDVIIDFEPQVTSRREGTALSDDGILARFYNNVAAEYLAQGEL
                     TLAYAHFKAAVLAAPGYSPSYSNLAQLYMRKGFLQSAEQLLLYAIALDDDSDIALRSL
                     HQLLVSQGRESEALKYKEILQARQTNDPYYWLGVGLHQLQEGNYQKAVNALERAQDLT
                     KGFLEVHRYLAIAYWRAGKPIQARNQLAVLASLMVGDLEFAALGRENQRSP"
     gene            complement(2087..3049)
                     /locus_tag="VEM33_01015"
     CDS             complement(2087..3049)
                     /locus_tag="VEM33_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYS50440.1"
                     /translation="MKVFASVTFAAALALAGVAQGQEKYSLSIATGGTGGVYYPLGGG
                     MANILSKYVPGLQATAEVTGGSVDNLKLIATGKPYIAFSMADAGQDAYRGEDKFKGAK
                     VPLRTLMVLYPNRMHVVTIEGIGITKMADLRGKRVSTGSPGSATEVMGFRVIEAAGLD
                     KDRDMKRERLGVAESVNALKDRKIDAFFWVGGLPTAAVTDLANTPGIKIRMIDHAETV
                     AAMNKKYGPLYIEDVIPKATYRGMDADNRQATVWNILVSHANLSNQTAYNIVKTIFDK
                     RDDMIAVHKEAENFRLENQKAAASPIPFHPGAVKYFAERGVKIR"
     gene            complement(3191..4318)
                     /gene="nadA"
                     /locus_tag="VEM33_01020"
     CDS             complement(3191..4318)
                     /gene="nadA"
                     /locus_tag="VEM33_01020"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HYS50441.1"
                     /translation="MTTTQVVEFERQLAGVACTARAWATIPMAPSVERRRELKARIKR
                     LLRERSAVLLAHYYVEPDIQDLAEETGGCVADSLEMARFGRAQAAPTLVVAGVRFMGE
                     TAKILNPEKRVLMPDLDANCSLDLGCPADEFSRFCDQYPERTVVVYANTSAAVKARAG
                     WVVTSAIALDVVRHLHERGERILWAPDRHLGAYVRSRTGADMLLWHGACVVHEEFKAL
                     ELEILKKEHPRAKVLVHPESPAEVIKLADAVGSTTQIINAAQALEADEFIVATDNGML
                     HKLRMLMPEKRFIEAPTAGASATCKSCAHCPWMAMNGLANLAEALETGANEIRIDPEI
                     ARRAYRPIDRMLAFAEERKVEVRASGDLARDGALFRNVGPA"
     gene            complement(4378..4980)
                     /locus_tag="VEM33_01025"
     CDS             complement(4378..4980)
                     /locus_tag="VEM33_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYS50442.1"
                     /translation="MTCNPGQRSAAAFAAALSFSMSCAAHAAEEVPPKAQACVACHGP
                     GGNATDPAMPSLAGQPAQFISMQLFLFRQGDRKDPQMSPIAANLSNADMNELASYFAS
                     QRPAAPSHKTAGENTAAGRRLAEQYHCVQCHGPALLGLQHIPRLAGQQFAYLRMQLRG
                     FKAKTRADVDGNMTSAAELLSEKDIEILADYLSGLTTARD"
     gene            complement(4980..>5264)
                     /locus_tag="VEM33_01030"
     CDS             complement(4980..>5264)
                     /locus_tag="VEM33_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HYS50443.1"
                     /translation="YTGKMFPAEYQNNIFIAMRGSWNRTVKQGYNVTRVSFDADGKPK
                     MEPFLTGFLTDEKADPPMWGRPNDVMVMRDGSLLVSDDQNGIIYRVSYEK"
BASE COUNT          956 a         1682 c         1623 g         1003 t
ORIGIN      
        1 aggaacgaag caagcacgac ggtgcggccg gcgctcgtgg actttccgcc catggcggag
       61 aacgagaaat cgatgaagaa cttgcccgct ccggaaaact gcaggaacgc cccatagatc
      121 gtgaacagaa tgatgagcga ggaagaaacg tccacggcga caccgaagat gccttcgagc
      181 gtcatataca gatgccccac gagacgaccc acttcgtagc tgcgatgcgc ccaggaaccg
      241 ggaagctgct cgccgaaaag cgcataaagg acgaagaagg taaccacgat cggcatgacc
      301 cagccggagg cgcggcgcat gccttcgagg atcaggagta tcagcgcgac gccgaagact
      361 aggtcccagt tgtccgggga ggtgttgcgg tcccagatat cgtcgccgcc ctggagaatg
      421 tacgcgatca ccgcgacggc ggcgaacgcg gccgcccaat cccaccacat gatgcggtgg
      481 cggtagcgtt tcgcgaccgg gaacagcagg tagctgagga aaagcacgaa gccgacgtgc
      541 accgggcgca gcacctgagt tggcacgatc gcgtacgcgc tgtagaggtg gaacagcgac
      601 atcgccaccg ccgtcgcgga gacgaagatg ccgagcgcgc cgcgcaactt gttgctcgcg
      661 ccctcctccg cctcgatgaa ctgctcggct tttttcagcg cctcgccgtc gattgccgac
      721 tcatcgggtc ccggcctcgc gatctcgctc atccgccctc ctcggcgcgc atgctagcac
      781 agcccgccag cgtgacacgc cgcctgcccg gctcattcgg cttggcaagc ctggcttcag
      841 gggctccgtt gattttctct gcccagcgcg gcgaactcca aatcgccaac catcaacgac
      901 gccagcactg cgagttgatt cctcgcctga atcggtttgc cggcccgcca atacgcaatg
      961 gccagatagc ggtgcacttc caggaatcct ttggtcaggt cctgcgcgcg ctcgagcgcg
     1021 ttgacggctt tctgataatt cccttcctgg agttgatgga gtccgacgcc gagccagtaa
     1081 tagggatcgt tcgtttgtct tgcctgcaga atttccttgt acttcaaggc ctccgattcg
     1141 cggccctggg ataccagcaa ttgatgcagg gagcgcagcg ctatatccga gtcgtcgtcg
     1201 agcgcaatcg cgtacaacaa cagttgttct gcgctctgaa ggaagccttt gcgcatatag
     1261 agctgcgcca gattgctgta gctcggtgaa tagcccgggg ccgcgagcac tgccgccttg
     1321 aaatgggcat aagcgagagt cagttcgccc tgcgccagat attccgccgc aacgttgttg
     1381 tagaagcgcg caagaatgcc gtcgtccgac aaagccgttc cctctcttcg cgaagtgact
     1441 tgaggttcga aatcgatgat cacgtcaccc gctcgcaaca atccgtcctt gaggtacagc
     1501 cctccaacgc ttctgccgct gatgagcagg ttcacgtgcc ggttgagaaa ctcgacgctg
     1561 ccgcgtcggt cgaagacgac ggggacgcgg acttcctgca tctggaccga catgtccagg
     1621 gctttcgcca gggaatagga caggacggtc agtgaaagac aatcaccgct tttccgacgc
     1681 cacgtttcag cggcgatcgt cgaatggttt ctcgaatagg aaaaatcttt cgtctgcggc
     1741 tcgaatagga gcgtgaccag atggttcaga cgatactgcg cgctagaatt ttgaattctt
     1801 ggatcgcgca gcttcgataa gagcccggcg tcaagctgaa agaggtcccg tttgccgacc
     1861 gagaccagag ccgcatcgta ctcaaaggcc tggtcctgcc acagaagatg cgcaggcagg
     1921 ggcgcaagcg gcgctgcggc acatcccgca agaacgataa gactccctcc gacccacggc
     1981 gccttgagca acatgaataa gctcgcccct cgcgaacatt ctgcgcctcg agcccatggt
     2041 cccgcaagcg gccccgcgcc ctggaagacg cgggccgtac ccagccttaa cgaattttta
     2101 cgccccgctc ggcaaaatac ttgaccgcgc cgggatggaa gggaatcggc gaagcggcgg
     2161 ccttctggtt ctccagcctg aaattctccg cctctttgtg cacggcaatc atgtcgtcgc
     2221 gcttgtcgaa gatggtcttg acgatgttat aggcggtctg gttgctcagg ttcgcgtgcg
     2281 aaacgaggat gttccacacc gtggcctgac gattgtccgc gtccatgccc ctgtaagtcg
     2341 ccttcgggat gacgtcctcg atgtagagcg ggccgtactt cttgttcatc gccgcgacgg
     2401 tttcggcgtg gtcgatcatc ctgatcttga tgccgggagt gttggcgagg tcggtcaccg
     2461 ccgcagtcgg cagcccgccc acccaaaaga acgcatcgat cttgcgatcc ttgagcgcgt
     2521 tcaccgactc ggcgacgccg agccgctcgc gcttcatgtc gcgatccttg tccaggccgg
     2581 ccgcttcgat gacgcggaac cccatcacct cggtcgcgct gcccggcgac ccggtcgaga
     2641 cgcgcttgcc cctgaggtcc gccatcttcg tgatgcctat gccttcgatc gtgaccacgt
     2701 gcatgcgatt gggatacaga accatcaggg tgcgcagggg caccttagcg cccttgaact
     2761 tgtcctcgcc gcggtacgcg tcctggccgg cgtccgccat ggagaacgcg atgtagggct
     2821 tgccggtcgc tatgagcttg aggttgtcca ccgagccacc cgtgacctcc gccgtcgctt
     2881 gcaggcctgg cacgtacttg gacaggatgt tcgccatccc gccgcccaac gggtaataca
     2941 cgccgccggt gccgccagtg gcgatcgaca agctgtactt ttcctgcccc tgggcaacgc
     3001 ctgccagcgc aagcgcggct gcgaaagtga ctgatgcaaa gaccttcatc gatttccctc
     3061 ctgtgaaagt ttgttcccca accgcttccg gccgctgctg cgggcctgtc cggttcaccc
     3121 gaaatatacg cggacgcccc ttccgcgcca agtggagcag cgctacctcg gcaaacaccc
     3181 gtgggccggt tcaggccggc ccgacattcc tgaagagcgc gccgtcccga gcgagatccc
     3241 cggacgcgcg cacctcgacc ttgcgctcct cggcaaaggc gagcatgcga tcgatcggac
     3301 ggtaagcacg gcgagcgatt tccggatcga tgcggatctc gttcgcgccg gtctcgagcg
     3361 cttcggcgag gttcgcgagg ccgttcatcg ccatccaggg gcaatgcgcg cagctcttgc
     3421 aggtggcgct tgcgcccgcg gtgggtgcct cgatgaagcg cttttcgggc atcagcatcc
     3481 tcagcttgtg aagcatcccg ttgtcggtcg cgacgatgaa ttcgtccgct tccagcgcct
     3541 gcgcggcatt gatgatctgc gtggtcgagc cgaccgcgtc ggcgagcttg atcacctcgg
     3601 cgggggattc cggatggacg agcaccttcg cccgcggatg ctctttcttc aggatctcca
     3661 gctccagcgc cttgaattct tcgtgcacga cgcatgcgcc gtgccacagc agcatgtcgg
     3721 cgcccgtccg gctccggacg taggccccga ggtgacggtc gggcgcccac agtatccttt
     3781 ccccgcgttc gtgcagatgg cgcacgacgt cgagcgcgat tgcggaggtg accacccagc
     3841 cggcgcgcgc cttgaccgca gcgctcgtat tcgcatagac caccacggtg cgctcggggt
     3901 actggtcgca gaaacgggag aattcgtcgg ccggacaacc cagatcgagc gagcagttcg
     3961 catcgaggtc gggcataagc acgcgcttct cgggattgag gatcttcgcc gtctcgccca
     4021 tgaagcgcac gcccgcgacc accagcgtcg gcgcggcctg cgcccgcccg aaacgcgcca
     4081 tctcgagcga gtccgccacg cagccgccgg tctcctcggc gaggtcctgt atgtcgggct
     4141 ccacgtagta gtgcgcgagc agcaccgcgc tccgctctct gagcaagcgc ttgatgcgag
     4201 ccttcagttc gcggcgccgc tccacgctgg gcgccatggg gatcgtcgcc cacgcccgcg
     4261 cggtgcacgc aaccccggca agctggcgtt cgaattcgac gacttgggtc gtggtcatgg
     4321 gggttggaca gttttcagag tgctgcgttg caacgatgtc gccgccgacc ccgcatatca
     4381 atcgcgggcc gtggtgaggc cggaaagata gtcggcgagt atttcgatgt ctttttccga
     4441 aagcagctcc gccgccgagg tcatgttccc gtcgacatcg gcccgggtct ttgccttgaa
     4501 accgcgcaac tgcattctca gataggcgaa ttgctgcccc gcgagccgcg ggatgtgctg
     4561 caggcccagg agcgccgggc cgtggcactg cacgcagtga tattgctcgg cgaggcgacg
     4621 tcccgccgca gtgttttccc ctgcggtctt gtgcgacggg gccgcgggtc tttgactggc
     4681 gaagtatgag gcaagctcgt tcatgtccgc gttggagagg ttggcggcga tcggcgacat
     4741 ctgcggatcc ttgcggtcgc cctggcgaaa aaggaaaagc tgcatcgaga tgaattgcgc
     4801 cggttgaccg gcgagtgacg gcatcgccgg gtcggtggca ttgcctcccg ggccatggca
     4861 ggcgacgcag gcctgcgcct tgggcgggac ttcttccgcc gcgtgtgcgg cgcaggacat
     4921 cgaaaaagaa agcgcggcgg caaatgccgc cgcgcttcgc tgaccgggat tacaggtcac
     4981 tacttttcgt agctcacgcg gtagatgatc ccgttctggt catccgagac gagcagcgag
     5041 ccgtcgcgca tcaccatcac gtcgttcggg cggccccaca tcggcggatc ggccttctcg
     5101 tccgtgagga accccgtcag gaacggctcc atcttcggct tgccgtcggc atcgaagctc
     5161 acgcgcgtga cgttgtagcc ctgtttgacg gtgcggttcc acgagccgcg cattgcgatg
     5221 aagatgttgt tctggtactc ggccgggaac atcttcccgg tgta
//
LOCUS       DASPZD010000054         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_456814, whole genome shotgun sequence.
ACCESSION   DASPZD010000054 DASPZD010000000
VERSION     DASPZD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456814"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VEM33_01035"
     CDS             complement(<1..449)
                     /locus_tag="VEM33_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS50444.1"
                     /translation="MKTTSSSRPLTLVHDSIEAKLAPLKILVIDDQSTGRIVLSEIMR
                     SLDTSLEVATFADPLEGIEYARKHPVDMVLTDYKMPSLDGIEVIRRLRRIFGYEDVPI
                     VCITVVNDREVRYRALEAGATDFLIRPIDRLECGARCRNLLSLRRHE"
     gene            complement(626..1984)
                     /locus_tag="VEM33_01040"
     CDS             complement(626..1984)
                     /locus_tag="VEM33_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYS50445.1"
                     /translation="MNLFAKLREREAQGRPLRVGMIGAGKFGAMYIAQVPRTPGVHLA
                     GIADLSPENARASLERVGWQPEQYAAASLDAALKERRTHVSEDWRALVAHPAVEIIVE
                     ATGNPIAAVEHALAAFRNGKHVVMVTVEADAFCGPLLAKKAKEAGVVYSLAYGDQPAI
                     ICDLVDWARAAGFPVVAAGRGHKWLPHFAQSTPETVWGYYGLTPEQARVGGLNPKMFN
                     SFLDGSKPAIEATAVCNATGLTPAPEGLAYPPVSIEDLPLVMRPKAEGGQLHHKGQVE
                     VVSSLDRDGRAIPYDIRFGVWVVFEGESDYIRRCFMEYGVRTDPSGRYASMYKRWHLI
                     GLEVGISVASVGLRGEPTGCATGFRADAVASAKRDLRAGEILDGEGGYMVVGRLMPAA
                     DSLALGGLPLGLAHQLKLVNPVKAGQAVRWADVAYDAGAEAVRVRREMESIFAAPKQR
                     AA"
     gene            complement(1981..2826)
                     /locus_tag="VEM33_01045"
     CDS             complement(1981..2826)
                     /locus_tag="VEM33_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYS50446.1"
                     /translation="MKLKLQQIQQFERDGYLFFPSLFRPEEIKVLLEEVPRLYAQRRP
                     ENVREKGSDAVRTNFAAHLYSYPFAKLARHPRMVEPIMQLFGEELYMHQFKINGKMAF
                     DGDVWQWHQDYGTWKNDDLMPEARAMNVAIFLDEVNEFNGPLMLIPGSHKLGVIEADH
                     DTSTTSYPLWTINHDTISKLVERGGIVAPKGPPGSMILFHSCLVHASTSNLSPWNRVS
                     VYLSLCAVSNHIRRFKRPGYIAHRDFAPLKCLPDDCLLKQYDVPLPWRNGTPPEELQG
                     ELRAA"
     gene            complement(2894..3211)
                     /gene="ftsB"
                     /locus_tag="VEM33_01050"
     CDS             complement(2894..3211)
                     /gene="ftsB"
                     /locus_tag="VEM33_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HYS50447.1"
                     /translation="MKILASTLAAFILLLQYPLWLGKGGWLRVWSLDQQVQEQKEANA
                     RLRQRNGALDAEVRDLKQGFEAIEERARYELGLIKQDEIFFQVVTPRAAPEIGLSPKD
                     GGR"
     gene            complement(3208..>4289)
                     /gene="eno"
                     /locus_tag="VEM33_01055"
     CDS             complement(3208..>4289)
                     /gene="eno"
                     /locus_tag="VEM33_01055"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYS50448.1"
                     /translation="EHINTEISEAIMGLDAQEQSFIDKTLIELDGTDNKSRLGANAIL
                     AVSMAVAKAAAEESGLPLYRYFGGSGAMQMPVPMMNVINGGAHASNNLDIQEFMIVPV
                     GAASFREALRCGAEVFHALKSLLESKRLSTTVGDEGGFAPNLPSNEAGLQLVLEAIER
                     AGYHAGTNVALALDCASTEFYRDGKYVLDSKKQRLGSAEFGEFLARWADKYPIVSIED
                     GMAESDWDGWRALTERLGKKVQLVGDDVFVTNTKILREGIQLGVANSILIKVNQIGTL
                     TETFAAIELAKRSGYTAVISHRSGETEDTTISDIAVGMNALQIKTGSLSRSDRVAKYN
                     QLLRIEEDLGDAVRYPGREAFYQLR"
BASE COUNT          728 a         1412 c         1311 g          838 t
ORIGIN      
        1 atttcgtgac ggcgcaggct caacagatta cggcagcgcg cgccgcattc gagccggtcg
       61 atcgggcgaa tcagaaaatc ggtagccccg gcctcgaggg cacggtaacg gacttcgcga
      121 tcgttgacca ccgtgataca cacgatcgga acgtcctcgt agccgaagat gcggcgcaag
      181 cgccggatga cctcgattcc atccaggctc ggcatcttgt agtcggtaag caccatgtcc
      241 acggggtgct tgcgcgcgta ttcgatgccc tccagggggt cggcgaaggt ggcgacctcg
      301 aggctcgtgt cgaggcttct catgatctcg ctcagcacga tgcgccctgt ggactggtcg
      361 tcgatgacca ggatcttgag cggcgcgagc ttggcctcga tcgagtcgtg aaccagcgta
      421 agcggtctag acgacgacgt ggttttcata agaatttcct tgcctcgcct ttgaacgggc
      481 gactccctgc cggccccccg ggtctaggcc caattacagt atcccaggcc tccctctctc
      541 gggtctaggc ccaattacac tatatttggc gggaaattgc gagatatccc gattcgttgc
      601 atttccggca ccgaaccctc gttctctagg ctgcgcgctg tttgggggcg gcgaatatcg
      661 actccatttc gcggcgcacc cgcaccgcct cggccccggc gtcgtaggca acatcggccc
      721 agcgcacggc ctggccggcc ttgacgggat tgactagctt cagttggtgc gccaaaccga
      781 gcggcaaccc gccgagcgca agcgaatccg ccgcgggcat cagcctgcct acgaccatgt
      841 atccgccttc gccgtcgaga atctcgccgg ccctgaggtc gcgcttggcg ctcgccactg
      901 catcggcgcg aaagcccgtt gcgcacccgg tcggctctcc ccgcaacccg accgaggcca
      961 ccgaaattcc gacctcgagc ccgatcaggt gccagcgctt gtacatcgac gcatatcgcc
     1021 ccgaagggtc ggttcgcacg ccgtattcca tgaagcaacg ccggatgtaa tcggactcgc
     1081 cctcgaacac cacccacacg ccgaagcgga tgtcgtaagg aatcgcccgc ccgtcgcgat
     1141 cgagcgagga cacgacttcg acctgcccct tgtgatgcag ctgcccgcct tcggccttcg
     1201 gtcgcatcac gagcggcagg tcttcgatgg acacgggggg atacgcgagc ccctccggcg
     1261 ccggcgtaag cccggttgca ttgcatactg cggtggcctc gatcgccggc ttcgagccgt
     1321 cgagaaaaga gttgaacatc tttgggttga gtccgcccac gcgggcctgc tccggcgtga
     1381 ggccgtagta gccccatacg gtctccggcg tggactgcgc aaaatgcggc agccatttgt
     1441 ggccgcgccc ggccgcgacc accgggaatc ccgccgcgcg tgcccaatcg acgagatcgc
     1501 agatgatcgc cggctgatca ccgtaggcga ggctgtacac gacgcccgcc tccttggctt
     1561 ttttcgcgag caggggcccg cagaacgcgt cagcctcgac cgtcaccatc accacgtgct
     1621 tgccgtttcg gaaggcagcg agggcatgct cgactgccgc gatcggattc ccggtggctt
     1681 cgacgatgat ctccaccgcc gggtgcgcaa cgagcgcacg ccagtcctcc gagacgtgcg
     1741 tgcgtcgctc cttcagcgcc gcatcgagcg acgcggcggc gtactgctcc ggctgccagc
     1801 ccacgcgctc gaggctggcg cgcgcattct ccggcgaaag gtcggcgatg cccgcgagat
     1861 gcacccccgg cgtgcgcggc acctgcgcga tgtacatggc gccgaatttg cccgcgccga
     1921 tcatgccgac ccgcaggggc ctgccctgtg cttcgcgttc ccggagcttt gcgaacagat
     1981 tcatgccgct ctgagctcgc cctgcagctc ttccggcgga gttccgttcc tccacggaag
     2041 cggcacgtcg tactgcttga gcagacaatc gtcggggagg cacttcaagg gcgcgaagtc
     2101 gcggtgcgcg atgtaccccg gacgcttgaa gcgccgtatg tggttcgaca ccgcgcacag
     2161 gctcagatac acgctgaccc ggttccaggg ggagaggttc gaagtcgagg cgtgcaccag
     2221 gcaggaatga aacagaatca tcgaccccgg cggacctttc ggagccacga tgccgccgcg
     2281 ctcgacgagc ttgctgatgg tgtcgtggtt gatcgtccaa agcgggtagc tggtcgtcga
     2341 ggtgtcgtgg tcggcttcga tcacgcccag cttgtggctg ccgggaatga gcatcagagg
     2401 cccgttgaac tcgttgacct cgtcgaggaa gatcgcgacg ttcatcgcgc gcgcctcggg
     2461 catcaggtcg tcgttcttcc aggtgccgta gtcctggtgc cactgccaga catcgccgtc
     2521 gaaagccatt ttgccgttga tcttgaactg gtgcatgtac aactcttcgc cgaagagttg
     2581 catgatgggc tcgaccatgc gcggatggcg cgcgagcttc gcgaaagggt agctgtacag
     2641 gtgcgcggcg aagttcgtgc gcaccgcgtc gcttcccttc tcgcgcacgt tctccggccg
     2701 gcgctgggcg taaagccgcg gcacctcttc aagcagcacc ttgatctcct ccggtctgaa
     2761 tagactcgga aagaaaagat agccgtcgcg ctcgaattgc tggatttgtt gcagcttgag
     2821 cttcaccggt gcgatttcct gtgcggagcc cgggatgagc agcaccgatt atagacccga
     2881 aaatgcctgg gcttcagcga cctccgtcct taggcgacag cccgatttcc ggggccgcgc
     2941 gtggggtaac gacttgaaag aagatctcgt cctgcttgat caatccgagc tcgtagcggg
     3001 cgcgctcctc gatcgcctcg aagccctgct tcaggtcacg cacctcggca tcgagcgcgc
     3061 cgttgcgctg gcgcagcctc gcgttcgctt ccttctgctc ctgcacctgc tggtcgagac
     3121 tccatacccg tagccatccg cccttcccga gccacaaggg atactgcaga aggaggatga
     3181 acgctgcgag agtagaggca agtatcttca tctcagctga tagaaggctt cccgccccgg
     3241 gtagcggacc gcgtccccaa ggtcttcctc gatacgcagc agctggttgt attttgcgac
     3301 ccgatccgag cgcgacagcg acccggtctt gatctgcagc gcgttcatgc cgacagcgat
     3361 gtcggatatc gtcgtgtcct cggtttcccc cgaccggtgc gaaatcacgg ccgtgtaacc
     3421 cgagcgcttc gcgagttcga tcgcggcgaa agtctcggtg agcgtcccga tctggttcac
     3481 cttgatgaga atggagttgg cgacgccgag ctggattccc tcgcgcagga tcttggtgtt
     3541 ggtgacaaag acgtcatcac ccaccagctg caccttcttg ccgagccttt cggtcagcgc
     3601 gcgccagccg tcccagtccg actcggccat cccgtcctcg atgctgacga tcgggtactt
     3661 gtccgcccat cttgcgagaa attctccgaa ctccgcggag ccgagccttt gctttttcga
     3721 gtcgagaacg tatttgccgt cccggtagaa ttcggtgctg gcgcaatcga gcgcgagcgc
     3781 cacgttcgtg ccggcgtggt aacccgcgcg ctcgatcgct tcgagcacca gctgcaaccc
     3841 cgcttcgttg ctcggcaggt tcggtgcgaa cccgccttcg tctccgaccg tcgtcgagag
     3901 gcgcttgctc tcgaggaggc tcttcagcgc gtggaaaacc tccgcgccgc agcgcagggc
     3961 ttcgcgaaag ctggccgcgc cgaccggcac gatcatgaat tcctggatgt cgaggttgtt
     4021 gctcgcgtgt gcaccgccgt tgatgacgtt catcatcggc accggcatct gcattgcgcc
     4081 cgagccgccg aagtagcggt atagcggcag gcccgactcc tccgccgcag ccttggccac
     4141 cgccatcgac accgcgagga tcgcgttcgc gcctaggcgc gatttgttgt cggtcccatc
     4201 tagctcgatc agggtcttgt cgatgaagct ctgctcctgc gcgtcgagcc ccatgatcgc
     4261 ctccgagatc tcggtattga tgtgctcga
//
LOCUS       DASPZD010000055         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1779894, whole genome shotgun sequence.
ACCESSION   DASPZD010000055 DASPZD010000000
VERSION     DASPZD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779894"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VEM33_01060"
     CDS             <1..611
                     /locus_tag="VEM33_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HYS50449.1"
                     /translation="GGQESSATRSGMADPVLRLAINLIGAPALSPEKFREYRQDTIVG
                     TSLVVSAPWGQYDGTKLVNIGTNRWSYKPEIGVSQGLGAWILEGAFGVTFFSDNDDFF
                     GGQTRKQDPLYAVQGHVIYAFNPGLWAALNSTYYAGGRTAVNGVLNNDLQQNSRWGAT
                     LSKSVDRRNSFKLYFSSGASARTGTNFTTLGFAWQHAWGAGL"
     gene            608..>2193
                     /locus_tag="VEM33_01065"
     CDS             608..>2193
                     /locus_tag="VEM33_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HYS50450.1"
                     /translation="MTVASSLRSAANWKFALAGAVIAVLAGAAIMWRAPGPAVPPGES
                     AARAAPEFVGSAACAGCHANETKAWTGSHHQLAMQEANAAAVLGDFDNARFRHYGVES
                     TFFRRDGKFMVRTDGPDGKLADFEVSRTFGVWPLQQYLIGFPGGRYQALGIAWDSRTK
                     SEGGQRWFHVYPNEKMDHRDPLHWAGLYQNWNLQCAACHSTNLKKGYDAAGNAYKTTF
                     SEINVACEACHGPGSRHLEWAKQARAPYLPQGDKGFVVLKTNWKDAWKFPAADAKHAQ
                     RDKPADAAAMNACAACHARRSTIAEGGTPGAPLEDTHRPALLTAPGYHADGQQREEVY
                     VWSSFLQTRMYQRGVTCMDCHEPHALKLREEGNALCTRCHSAALFDTDKHHFHKAGTK
                     GAQCVECHMPAQNYMVIDARRDHGIRVPRPDLSASLGSPNACTQCHAGRKPEWAAAAL
                     DQWYGQPWRARAQYGTTLHAATTQGAKALPSLLALANDPAAPSIVRATAVALVQPYVN
                     PANLPALRNLLAHSDPSLRIA"
BASE COUNT          402 a          735 c          721 g          335 t
ORIGIN      
        1 tcggggggca ggagtcgagc gcgacgcgca gcggaatggc cgacccggtg ctgcgcctgg
       61 cgatcaatct gatcggggcg cccgcgctct ctccggaaaa attccgcgag taccggcagg
      121 acaccatcgt cggaacgagc ctcgtggtga gcgccccgtg ggggcaatac gacggcacga
      181 agctcgtcaa catcggcacc aaccgctggt cgtacaagcc cgagatcggc gtgtcccagg
      241 ggctcggcgc atggatcctg gaaggagcct tcggggtcac cttcttcagc gacaacgatg
      301 atttcttcgg cggacaaaca cgcaagcagg atcccttgta tgcggtgcag ggacacgtga
      361 tttacgcctt caatccgggg ctgtgggcgg cactcaattc gacctactac gccggcggcc
      421 gcacggcggt gaacggcgtg ctcaacaacg atttgcagca gaactcgcgc tggggcgcga
      481 cgctttcgaa atcggtggac cggcgcaact cgttcaagct ctacttcagt tccggcgcga
      541 gcgcgcgcac cgggaccaac ttcacgacgc ttggatttgc ctggcagcat gcctggggcg
      601 ccgggctatg acggttgcaa gctcgcttcg atcggccgcg aattggaagt tcgcgctggc
      661 gggtgcagtc atcgccgtgc tcgcgggcgc ggcgatcatg tggcgcgcac cgggccccgc
      721 ggtcccaccc ggcgagagcg cggcacgagc cgcgcctgaa ttcgtcggga gcgcggcttg
      781 cgccggatgc cacgcgaacg agaccaaagc gtggaccggc tcgcaccacc agctcgcgat
      841 gcaggaagcg aatgccgccg cggtgctcgg cgacttcgac aacgccaggt tcaggcacta
      901 cggcgtcgag tcgactttct tcaggcgcga cggcaagttc atggtgcgca ccgacggacc
      961 cgacggcaag ctcgccgact ttgaagtgag ccgcaccttc ggcgtctggc cgctgcagca
     1021 atacctgatc ggttttcccg gcgggcgcta tcaggcgctc ggcatcgcgt gggattcgcg
     1081 gacgaagagc gagggcgggc agcgctggtt ccatgtctat ccgaacgaga agatggatca
     1141 ccgggatcca ttgcactggg ccgggctgta tcaaaactgg aatctgcagt gcgctgcgtg
     1201 ccactcgacg aatctgaaga agggctatga cgccgccggc aatgcctaca agaccacctt
     1261 cagcgagatc aacgtcgcct gcgaggcttg ccacggcccg ggctcgcgcc accttgaatg
     1321 ggccaagcag gcgagggcgc cctacctgcc gcaaggcgac aagggtttcg tggtgttgaa
     1381 aacgaactgg aaagatgcgt ggaaatttcc ggcggcggat gcgaagcatg cgcagcgcga
     1441 caagcctgcc gatgcagccg caatgaatgc ctgcgcggcg tgccacgcgc gccgctcgac
     1501 gatcgccgaa ggcgggacgc ccggcgcgcc gctcgaagac acgcaccgcc cggcgctgct
     1561 gaccgcgccg ggctaccacg ccgatgggca acagcgcgag gaggtctacg tctggagctc
     1621 gttcctgcag accaggatgt accagcgcgg cgtcacttgc atggactgcc acgagccgca
     1681 cgctctgaag ctgcgcgaag agggcaacgc cctgtgcacc cgctgccata gcgccgcgct
     1741 gttcgacacc gacaagcatc attttcacaa ggccggaacg aaaggcgcgc aatgcgtcga
     1801 gtgccacatg ccggcgcaga actacatggt gatcgacgcg cggcgcgacc acggcatccg
     1861 cgtgccgcgt cccgacctgt cggcctcgct cggcagcccg aacgcctgca cccaatgtca
     1921 cgcagggcgc aagcccgaat gggccgcggc cgcgctggac cagtggtacg gccagccctg
     1981 gcgcgctcgc gcgcagtacg gaaccacgct ccatgccgcc acgacgcagg gcgcgaaggc
     2041 gctgccctcg ctccttgcgc tcgccaacga tccggcagct ccttccatcg tcagggcgac
     2101 cgcggtcgcg ctggtgcaac cctatgtcaa tcccgcgaac ctgcctgctc tgcgcaatct
     2161 gctcgcccac tcggacccgt ccctgcgtat cgc
//
LOCUS       DASPZD010000056         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1077581, whole genome shotgun sequence.
ACCESSION   DASPZD010000056 DASPZD010000000
VERSION     DASPZD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077581"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VEM33_01070"
     CDS             complement(<1..805)
                     /locus_tag="VEM33_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYS50451.1"
                     /translation="MRALLGDRVTTSRGVREHHGKDESYFPYAPPDAVVFPESTEEVR
                     GIVNLCRRHKTPMIPYGVGTSLEGHVLAIHGGVCIDLSRMNRVLAVHDADLDSVVQAG
                     VTRKQLNEHIRHTGLFFPVDPGADATLGGMAATRASGTNAVRYGTMRENVLALTVVLA
                     DGRVITTSRRSRKSAAGYELTRLFVGSEGTLGIITELTVRLYAVPEAMSAAVCSFQEI
                     AGAVNTVIQTLQAGIPIARSEALCATTMKAINAYSKTSYREQPTLFLEFH"
     gene            complement(863..1069)
                     /locus_tag="VEM33_01075"
     CDS             complement(863..1069)
                     /locus_tag="VEM33_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HYS50452.1"
                     /translation="MNEDAFNLSIRKFLKMVGVSSQREIEQAVAKARAGGAIKGNEKF
                     PASVTLEVPGVKLSVRFDGKIELE"
     gene            complement(1114..2691)
                     /gene="pgi"
                     /locus_tag="VEM33_01080"
     CDS             complement(1114..2691)
                     /gene="pgi"
                     /locus_tag="VEM33_01080"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HYS50453.1"
                     /translation="MSKLTLSPAWKALAAHRERTAATSLADLFAGDPSRAERFSIEAG
                     ELVLDYSKHRVDAETMRLLAGLAAQAQLASWIARMFAGDPINSTEARAALHVALRSGD
                     ATFPERGDVMPAVRGALESMRRFVGDVHGGVLKGADGRAITDVVSIGIGGSDLGPRML
                     ARAMRKFPKGGPKLHFVSNIDPADLEAALAGLAPDTTFFIVASKTFTTAETLANAARA
                     RAWLEAALGDSNGPQRHFAAVTANAAGAEALGIPRERVFPMWDWVGGRYSVWSAVGLP
                     VALAAGMNAFEELLEGARAMDAHFRNEPLERNMPVVLALLEIWYVNFFGAQTRAVIPY
                     SEDLRDLPAYLQQLEMESNGKRVDRDGNEVDYATAPAVWGASGTPSQHSFHQLLHQGT
                     HLIPVDFIVIGRNGEPSSGQDALVANALAQSAALAFGNPAPGAPHKALPGNRPSSTLL
                     LERLTPRALGALIALHEHKVFAEGVIWNLNSFDQWGVEHGKNLARTLLPRLLDGGDAA
                     GLDSSTRSLLARLRRRD"
     gene            complement(2688..>3010)
                     /locus_tag="VEM33_01085"
     CDS             complement(2688..>3010)
                     /locus_tag="VEM33_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYS50454.1"
                     /translation="ATTLLLSWVDYGKSYRTVAQSLGKNIPADARCIESRGLGEPQRG
                     VFDYHAEVVTQRLERGAGARCSVLLVQAREGESDDIGPGWKLLWEGRRPRDRERFRLY
                     QRQP"
BASE COUNT          470 a          996 c         1020 g          524 t
ORIGIN      
        1 cgtggaattc gagaaacagc gtcggctgct cgcggtagct cgtcttcgag taggcgttga
       61 tggctttcat ggtcgtagca cacagcgcct cgctgcgtgc gatcggaatc ccggcctgca
      121 gggtctggat caccgtgttc accgcgccgg caatctcctg aaacgagcag acggccgccg
      181 acatcgcctc cggaacggcg taaaggcgta ccgtgagctc ggtgatgatt ccgagcgtgc
      241 cttccgatcc cacgaacagc cgcgtcagct cgtagcccgc ggcggatttg cgcgagcggc
      301 gcgaggtggt aatgacgcgg ccgtcggcga gcacgacggt cagggcgagc acgttctcgc
      361 gcatcgtgcc gtagcgcact gcgttcgtgc ccgaagcacg cgtcgcggcc atgccgccca
      421 gcgtcgcgtc cgccccgggg tcgacgggga aaaaaaggcc cgtatgccgg atgtgctcgt
      481 tcaattgctt ccgcgtcacg cctgcttgga ccaccgagtc caagtcggcg tcgtgcacgg
      541 cgagcaccct gttcatgcgt gaaaggtcga tgcacacccc gccgtggatg gcgagcacgt
      601 ggccctcgag cgaggtgccg acgccgtagg ggatcatcgg cgttttgtgg cgccggcaga
      661 ggttgacgat gccgcgcacc tcctcggtgg attcgggaaa caccaccgca tcgggcggcg
      721 cgtacggaaa gtacgactcg tctttgccgt gatgctcgcg cacgccccgc gaggtcgtga
      781 cgcgatcgcc gagcagcgcc cgcatctctt cgagcagcgc agcgtcgaat tcgctgggct
      841 tgttcaagac gatacctcgc gactactcga gttcgatctt gccgtcgaac cgcaccgaga
      901 gctttacgcc gggaacttcg agcgtgaccg aggcggggaa cttctcgttg cccttgattg
      961 cgcccccggc gcgcgccttc gccacggcct gctcgatctc cctttgggaa ctcactccga
     1021 ccatcttcag gaactttcgg atgctgaggt tgaaggcgtc ttcgttcatg atggtctccg
     1081 tttgcatcag cgtaagtccc gccgcggccg gagtcaatcg cgtcttcgca gccgcgcgag
     1141 caggctccgc gtcgacgagt cgagtcccgc cgcgtctccg ccgtcgagca gtctcggcag
     1201 cagagtgcgg gcgagattct tgccgtgctc caccccccat tgatcgaagc tgttcaggtt
     1261 ccagatgacg ccttcggcga acactttgtg ctcatgcagc gcgatgagtg cgccgagcgc
     1321 gcgcggcgtg aggcgctcga gaagcagggt gctcgaaggg cggttgccgg ggagggcctt
     1381 gtgcggcgcg cccggcgcgg ggttgccgaa ggcaagggct gcgctctggg cgagcgcatt
     1441 cgccacgagc gcgtcttgcc cggatgacgg ttccccgttt cgtccgatca cgatgaaatc
     1501 caccgggatg agatgggtcc cttggtgcag cagctggtga aaggagtgct ggctgggcgt
     1561 gcctgaggcg ccccagacgg cgggagccgt ggcgtagtcg acctcgttcc cgtctcggtc
     1621 cacgcgcttc ccgttcgatt ccatctcgag ctgctgaagg tacgccggca aatcgcgcag
     1681 atcctcgctg taagggatca cggcacgggt ctgggcgccg aagaagttca cgtaccagat
     1741 ctcgagcagc gcgagcacca ccggcatgtt gcgctccagg ggctcgttgc ggaaatgggc
     1801 gtccatcgcg cgtgcgccct caagcagctc ttcgaacgcg ttcatgccgg ccgccagcgc
     1861 gaccggcaga cccacggcgg accagaccga atagcgcccg ccgacccagt cccacatcgg
     1921 gaacacgcgc tcgcgcggaa tccccagtgc ttcggcgccg gcggcgtttg cggtgaccgc
     1981 cgcgaagtgg cgctgcggcc cgttcgagtc tccgagcgcc gcctcgagcc aggcgcgagc
     2041 gcgggccgca ttcgcgaggg tctcggcggt ggtaaaggtc ttcgatgcga cgatgaagaa
     2101 agtcgtatcc ggcgctagac ccgcgagtgc ggcctcgagg tcggccgggt cgatgtttga
     2161 gacgaaatgc agcttcggcc cgcccttggg gaacttgcgc atcgcccgcg cgagcatgcg
     2221 gggcccgagg tccgagccgc cgatcccgat gctgaccacg tcggttatcg ctcggccgtc
     2281 ggcgccttta agtacgccac cgtgcacgtc acccacgaag cggcgcatgc tttcgagcgc
     2341 gccgcgcacg gcgggcatca catcgccgcg ctcggggaat gtcgcatccc cggaacgcag
     2401 cgccacatgc agcgcggctc gtgcctcggt gctgttgatg gggtcgcccg cgaacatgcg
     2461 cgcgatccag ctggccagct gcgcctgggc ggcgaggccc gcgagcagcc gcatcgtctc
     2521 cgcatcgacc cggtgcttgg aataatcgag cacgagctcc ccggcttcga tcgagaatcg
     2581 ctcggcgcgc gaggggtccc cggcgaacag gtcggcgagg cttgtagccg cggtcctctc
     2641 tcggtgcgcg gccagcgctt tccatgcggg cgacaaggtc agcttgctca cggctggcgc
     2701 tgatagaggc ggaagcgttc gcggtcgcgc ggccgcctgc cttcccacaa gagcttccac
     2761 ccggggccga tgtcgtccga ttctccttcg cgcgcttgta ccaggagcac cgagcagcgg
     2821 gccccggcgc cgcgctcgag gcgttgtgtg acgacctcgg cgtgatagtc gaatacgcca
     2881 cgctggggtt cgcccaggcc acggctctcg atgcagcggg cgtccgcggg aatgttcttt
     2941 cccagcgact gcgcgaccgt ccggtagctc tttccgtagt cgacccagga gagaagcaag
     3001 gtcgtggcga
//
LOCUS       DASPZD010000057         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_353410, whole genome shotgun sequence.
ACCESSION   DASPZD010000057 DASPZD010000000
VERSION     DASPZD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353410"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VEM33_01090"
     rRNA            <1..497
                     /locus_tag="VEM33_01090"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            695..809
                     /gene="rrf"
                     /locus_tag="VEM33_01095"
     rRNA            695..809
                     /gene="rrf"
                     /locus_tag="VEM33_01095"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            910..1143
                     /locus_tag="VEM33_01100"
     CDS             910..1143
                     /locus_tag="VEM33_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYS50455.1"
                     /translation="MRRNLVFVKGPIIISDPDILGGTPVFHGTRVPVQTLFDYLEAGD
                     TIDDFLEGFPSVTREHVIAFLEEAKNRLIESAT"
     gene            1434..2661
                     /gene="bioA"
                     /locus_tag="VEM33_01105"
                     /pseudo
     CDS             1434..2661
                     /gene="bioA"
                     /locus_tag="VEM33_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
     gene            2661..>2905
                     /locus_tag="VEM33_01110"
     CDS             2661..>2905
                     /locus_tag="VEM33_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS50456.1"
                     /translation="MAIGGTGASVFVTGTDTGVGKTLVSCAMLHRLRACGLRALGMKP
                     VAAGVEATPEGPVNPDVVALRAASSWPAPLSQVNPYC"
BASE COUNT          501 a          900 c          941 g          563 t
ORIGIN      
        1 atggaagggc catcgctcaa cggataaaag gtactctggg gataacaggc tgataccgcc
       61 caagagttca tatcgacggc ggtgtttggc acctcgatgt cggctcatct catcctgggg
      121 ctgtagccgg tcccaagggt atggctgttc gccatttaaa gaggtacgtg agctgggttt
      181 aaaacgtcgt gagacagttt ggtccctatc tgccgtgggc gctggaagat tgacgggggc
      241 tgctcctagt acgagaggac cggagtggac gcacctctgg tgtaccggtt gtcacgccag
      301 tggcatcgcc gggtagctat gtgcggaaga gataaccgct gaaagcatct aagcgggaaa
      361 ctcgcctgaa gattagtctt cccggaggct tgacctccct gaagggtcgt ccaagaccag
      421 gacgttgata ggctggatgt ggaagggccg tgaggccttg agctaaccag tactaattgc
      481 ccgtgcggct tgatcctata accgtgccgc ctcgttctcg aggctgaggc tcgaaggaat
      541 cgatcattga cccaccctgc tccgcgctag cgcgctacgc agggcaagct ttgcaccctg
      601 cgtagcgcga cagcgcgaag cagggcttcc gagttggatc tttttggcgc agcgcctgaa
      661 cggttgctgc agccgaaagg tcgtctgtct tcagcctgac gaccatagcg aggtggcacc
      721 accccttccc atcccgaaca ggaccgtgaa acgcctcagc gccgatgata gtgggatgta
      781 tttcccgtga aagtaggaca tcgtcaggct aactatccga acgccccgcc gtgaaccggc
      841 ggggcgtttc gctttttgcg ggaagtgtgg cgaactggcg gcaaggtcgt aggttgcctg
      901 ccgaccccga tgaggcgtaa tctggttttt gtgaaagggc cgatcatcat cagtgacccc
      961 gatattctgg ggggaacacc ggtgttccac gggacgcgag tgccggtgca gacactattc
     1021 gattacctcg aggcgggcga caccatcgat gatttcctcg agggctttcc gtccgtgacg
     1081 cgggagcatg taatcgcctt tttggaggaa gcgaagaacc gactgatcga atcggcgacg
     1141 tgaaggttct tctcgacgag tgcgttgact ggcgcctgtc gcgcgagttt cagggcaaga
     1201 cgaatcggct tgcagacctt ctaccgttgg tcccgcgtct cctggcggcc atcgagtccg
     1261 ctttgcctgg ctctcccaaa tttatcggcc ccgtctgagg tcgagtcgcg cccgctcgat
     1321 cgcgctcggg tcgggctgcc tagaagatga ccacgaatcg cgacctcgtt gcccgcacgc
     1381 tgcgctcggt ctggcacccg tgcacgcaga tgaagctgca tgagcaactg ccgatggtgc
     1441 ccgtatcgca cggcaaaggc gcctggctgt acgacttcga ggatcgccgc tacctggacg
     1501 cgatcagctc ctggtgggtc aacctgttcg gccacgcgaa ttcccgcatc aacgcggcga
     1561 tcgtcgctca actcggacgg ctcgaacatg tgatcctggc cggcttcacg cacgagccgg
     1621 cggtggtgct ttccgaacgc ctgtcggcct tgaccggtgg gcggctagga cactgcttct
     1681 tcgcctccga cggctcctcc gccgtcgaga tcgcgctcaa aatgagtcac catttctggc
     1741 gcaatcgcgg tcgaccggac aaatcccgct tcgcctacct gaccggtgcc taccacggcg
     1801 aaaccaccgg cgcgctgtcg gtcggcgatg tcgcgctgtt tcgcgacgcc tacgcgccgc
     1861 tgcttcgggc cggaatcgcg gtgcccagcc cggactggcg ccatgccgaa tcgggagaat
     1921 cggctccgca gtacgcgagg cgctgcgcga tcgcactcga gcagacgctg cgcgagtggc
     1981 accgcgaaat cgccgcgctg atcgtcgagc cgctcgtgca gtgcgccagc gggatgggca
     2041 tgtacgaccc cgagtacctg cgccttgcgc gcgcgatctg acaagtacaa cgtgcatctg
     2101 atcgccgacg agatcgcagt cggctgcggc cgcaccggga ccttcctggc ctgcgagcag
     2161 gccggcatcc tgcccgacct gttgtgcctg tcgaaaggca tcagcgccgg ctatctgccg
     2221 ctgtccgtgg tgctgtcgac gcacgcgatc tacgaggcgt tttacgacga cgaagtctcg
     2281 cgcggcttcc tgcactcgca ctcgtacagc ggcaatccgc tcgcctgcgc ggcggcgctc
     2341 gccaccttgt cgatcttcga ggaagattcg gtgatcgaaa gcaaccggca gcgcgccgcg
     2401 cggatcgacg agctggcgcg tccgatcgcc gcgcacccgc gtgttcgcaa tttccggcgc
     2461 agcgggatga tctgggcttt cgaggtcgac ggcgcgcagc cgctgtttgc gcgccgcttc
     2521 tccgctgccg cgctcgagcg cgagctgctg ctgcgcccga tcggcgagac ggtctacttc
     2581 atgccgccgt acgtgatcga cgatgagcag gcggcgctgt tgaccggccg cacggtcgaa
     2641 cttctcgatc gggtaccgtg atggccatag ggggtaccgg cgcaagcgtt ttcgtgaccg
     2701 gaaccgacac cggcgtgggc aagacgctgg tcagctgcgc gatgctgcac cggctgcgag
     2761 cgtgcggttt acgcgcgctc ggcatgaagc cggtggcggc cggcgtggag gcgacgccgg
     2821 agggacccgt caatccggac gtggtcgcgc tgcgcgcggc aagctcgtgg ccggcgccgc
     2881 tgagccaggt gaatccctac tgttt
//
LOCUS       DASPZD010000058         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_571845, whole genome shotgun sequence.
ACCESSION   DASPZD010000058 DASPZD010000000
VERSION     DASPZD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571845"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="VEM33_01115"
     CDS             complement(<1..1989)
                     /locus_tag="VEM33_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYS50457.1"
                     /translation="MKVSEMKDAVFDGRNMGYVPPKKLSVSPKLKLHRKGAKNIDPIT
                     YEVIRHSLWHVNEEHGATIQRLSGSPVAMYALDLNPSILTEDGEFVYFGPYMQYMSGV
                     TDTQVKWVLEYRSDNPGIAEGDMFLANDPWVGAAHQMDVMLLCPVFWKGELFCWVTNC
                     LHQYDLGGITPSSFCGSAENAFEEGICIPPVKIVEGNEIRRDIEELYLRASRKPEAVA
                     LDFRAQLAGNITARERVLALIRRYGPEVVKGVMRKILDNGERAFLDKLKRLPDGVWRE
                     RSYVECCRPGDRRTHQVMLTLRKNGSTLIFENEGTAPQDGAMNATYSGWRGAIMVAFN
                     QLLCWDQYFSIGGALRHVEFDPTPGTFNCANFPASVSTAPIQAMEISLYPAYNVLSKM
                     IHADPEMRKDIMCIGGTSQWPATIFRGTDQWGERYGYILVDPIGGAIGAFAGGDGIST
                     GGQSRTPICKLPNVEHTEQTFPLLFLYRKEVIDSGGAGKFRGGLSAESCFIPHRTESI
                     TQDTLSSGNAIPTSPGMMAGYPGSVNVYKFKRATDIFERLRERRIPGDIEELKGEEVT
                     LALREENFLQKPDDVYAVIWSAAGGFGDPLERDPEKVRDDVIEQRSVSMDAARDLYGV
                     VIARDGRLDGEATRDLRAERREAHRRKDGEVKRLDGERL"
     gene            complement(1986..>2996)
                     /locus_tag="VEM33_01120"
     CDS             complement(1986..>2996)
                     /locus_tag="VEM33_01120"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYS50458.1"
                     /translation="PVCYGKGGTIATVTDADVVLGTINPDNFLGGRIKLDRKKAVDSV
                     QRIASALGLSLTEAASGIARIAEFKMADIIRKTTVEKGFDPRDFVLFAFGGAGPAHAG
                     VFARELGVQKVIVPQKEIASTWCAFGAASADILHVYEQVDIQASPFDAARVNASLEAL
                     EKRASEQMDRDGIAAPRRHYQFSLDMRHKGQINEVEVMLPEKRVSGSLWKALHQRFYA
                     RYEQLYGSGSSYGEARLEIVTLRLRATAATPRPKLSRAKKLSTKIDSKANCGKRSIYW
                     ADLKRPAATPILDGAFLVPGNAVKGPAVIETTDTTVVVHPGRSLKVDAYGNFEINFGE
                     RA"
BASE COUNT          485 a          996 c          953 g          562 t
ORIGIN      
        1 gagccgctct ccgtcgagcc gcttcacctc gccgtccttc cggcggtggg cctcgcgccg
       61 ctccgcgcgc aggtcccggg ttgcttcccc gtcgagccgg ccgtcccgcg cgatcacgac
      121 gccgtagaga tcgcgcgcgg catccatcga cacggagcgt tgctcgatca cgtcgtcgcg
      181 cactttttcc ggatcgcgct cgagcggatc gccgaagccc cccgccgcgg accagatcac
      241 cgcgtagacg tcgtcgggtt tctggaggaa gttctcctcg cggagcgcga gcgtcacctc
      301 ctcgcccttc agctcctcga tgtcgccggg aatgcggcgc tccctcaggc gctcgaaaat
      361 atcggtcgcc cgcttgaact tgtacacgtt caccgagccg ggatagcccg ccatcatccc
      421 gggcgaagtc gggatcgcat tgcccgagga cagcgtgtcc tgcgtgattg attcggtccg
      481 atgcggaata aagcaggact ccgccgagag cccgccgcgg aatttccccg cgccgccgga
      541 gtcgatcact tccttgcggt aaaggaaaag cagcggaaag gtctgctcgg tatgctcgac
      601 gttgggcagc ttgcagatcg gcgttctgga ctgccctccc gtggaaatgc cgtccccgcc
      661 ggcaaaggcg ccgatcgcgc cgccgatggg atcgacgagg atgtagccgt agcgctcgcc
      721 ccactggtcg gtgccgcgga aaatcgtcgc cggccactgg ctggtgccgc cgatgcacat
      781 gatgtccttt cgcatctcgg ggtccgcgtg gatcatcttc gagagcacgt tgtacgcggg
      841 gtagagcgag atctccatcg cctggatcgg cgcggtggaa accgaagccg ggaaattggc
      901 gcagttgaaa gtccccgggg tcgggtcgaa ctcgacgtgg cgcagcgccc cgccgatcga
      961 gaaatactgg tcccagcaca gcagctggtt gaacgccacc atgatcgcgc cgcgccagcc
     1021 cgagtaggtc gcgttcatcg cgccgtcctg gggcgccgtg ccttcgttct cgaaaataag
     1081 cgtcgaacca ttcttccgca gcgtcaacat gacttgatgc gtgcgccggt cgccgggacg
     1141 gcagcattcg acatagctgc gctcgcgcca caccccgtcg ggcaggcgct tcaatttgtc
     1201 gagaaaggcg cgttcgccgt tgtccaggat cttgcgcatc acgcccttca cgacttcagg
     1261 cccatagcgc cggataagcg caagcacccg ttcgcgcgcg gtgatgttgc cggcgagctg
     1321 cgcccggaag tcgagcgcga ccgcttcggg cttgcgcgag gccctcaggt acagctcctc
     1381 gatgtcgcgg cgtatctcgt ttccttcgac gatcttcacc ggcgggatgc agatgccttc
     1441 ttcgaaggcg ttctccgccg agccgcagaa cgatgacggc gtgatcccgc cgaggtcgta
     1501 ctggtgcagg cagttcgtca cccagcagaa cagctcgcct ttccagaaca ccgggcacag
     1561 cagcatcaca tccatctggt gcgccgcgcc gacccagggg tcgttcgcga ggaacatgtc
     1621 gccctccgcg atgccgggat tgtcgctgcg gtactcgagg acccacttca cctgcgtgtc
     1681 ggtgacgccc gacatgtact gcatgtaggg cccgaagtac acgaactcgc cgtcctcggt
     1741 gaggatggaa ggattgagat cgagcgcgta catcgccacc ggcgacccgg agaggcgctg
     1801 gatcgtggcg ccgtgctcct cgttcacgtg ccaaaggctg tgccggatca cctcgtaggt
     1861 gatcgggtcg atgttcttcg cgccctttcg atgcagcttg agcttgggcg agacggaaag
     1921 cttcttgggc ggcacgtaac ccatgttcct gccgtcgaag acggcgtcct tcatttcgct
     1981 tactttcatg cccgttcccc gaaattgatt tcgaagttgc cgtaggcgtc caccttgagc
     2041 gagcgccccg gatgcacgac gacggtggta tcggtggtct cgatgacggc cgggcccttg
     2101 accgcgttcc ccggaacaag gaaggcccca tcgaggatcg gcgtcgccgc cggccttttc
     2161 aggtcggccc agtagatgct tcgcttgccg cagttcgctt tcgaatcgat tttcgtggag
     2221 agttttttcg ccctcgagag cttcggccgg ggcgtcgcgg cggtcgcgcg caggcgcagc
     2281 gtcacgatct ccaggcgcgc ctcgccgtag gacgagccgc tcccgtacag ctgctcgtag
     2341 cgcgcataga agcgctggtg cagcgccttc cagagcgagc cgctgacgcg cttctccggg
     2401 agcatcacct cgacttcgtt gatctggccc ttgtggcgca tgtcgagcga gaactgatag
     2461 tgccgccgcg gcgcggcgat gccgtcgcgg tccatctgct cgctcgccct tttctccagt
     2521 gcctcgagcg aggcgttcac gcgcgccgcg tcgaacgggc tcgcctggat atcgacctgt
     2581 tcgtacacgt gcaaaatgtc ggccgaggcc gcgccgaagg cgcaccaggt cgaggcgatc
     2641 tccttctgcg ggacgatcac cttttgcacc ccgagctcgc gggcgaacac gcccgcgtgc
     2701 gccgggcccg cgccgccgaa agcgaagaga acgaagtcgc gcggatcgaa acccttctcg
     2761 acggtggtct tgcggatgat gtcggccatc ttgaactcgg cgatccgggc gatgccgctc
     2821 gcggcctccg tgagcgaaag gccgagcgcc gaggcaatgc gctgcaccga gtccacggcc
     2881 ttcttccggt cgagcttgat ccgcccgccg agaaaattat ccggattgat ggttccgagc
     2941 accacgtcgg cgtcggtgac ggtggcgatc gtgccgccct tgccgtagca aacagg
//
LOCUS       DASPZD010000059         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1054243, whole genome shotgun sequence.
ACCESSION   DASPZD010000059 DASPZD010000000
VERSION     DASPZD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054243"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2442)
                     /locus_tag="VEM33_01125"
     CDS             complement(<1..2442)
                     /locus_tag="VEM33_01125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HYS50459.1"
                     /translation="MYRTAAGSLALAAALLLCCIASAGAEPRFSFATTPGKLPKDVVP
                     RHYALRIVPAPTYDRFDAEAVIDIEVTRPVPAIVVNAAELSFKSVKLRAGTGDETSLA
                     PRFDPQRETVTLTPSTAPIAPGSYRLGIEYSGKIGKHPQGLYQIAYKERDRGRLVDKL
                     MLATQMEPVQARRLFPGWDEPVFRASFDIVTVIDATLTAVSNMPQSKVELRPDGRKEV
                     SFARSVPMSAYLVALFVGEMDLLEDSVDGIRLGIHTVKGKRERARYAMEATKQIVRYF
                     NAYFGEPYPLVKLDQLALPGGISGAMENWGAIAYNEGRFLYSPGEDSLRQQQAVYGII
                     AHEIAHQWFGNLVTMAWWDNLWLNEGFASWMAGKTTERFNPRWGMPLRDALWKESALS
                     EDARRMTHPIQTPVENDTRAMDVFDSITYAKGAAVLRMLEGYLGEGVFRAGVQGYMRA
                     HRFSNTTTADFWHHLSGASGQDIGKLVAGWTEQPGYPVVKVSQECEKGSAAVTLAQER
                     FTLNDPGAAPLAWNVPVILADAAGTRRTVLLERNPQKLRFERCGAMLANAGDTGYYRS
                     QYDDGNFGRLVLVLQELPAPDRLRLLSDTFALSQAGRVDVTRYLALVESLGAETDRTI
                     WDQVTSALRFLRELIDSPGDQAVFDRYVARLLEAPFARAGWDVRPGEAADAALLRRSL
                     IEALGRAGHEAVVREAQSRFAARAEKLIDPAIRPAVLNVIGRYADEATFEALLERMRS
                     AVDMTDKWEVQAALRQVRDPRLLQRLMELLLTDELPPSDAVFNLTHIGEDSGRVELVW
                     RFVLEHLPAVLAKASP"
     gene            complement(2466..2675)
                     /locus_tag="VEM33_01130"
     CDS             complement(2466..2675)
                     /locus_tag="VEM33_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50460.1"
                     /translation="MRMNRVISGISMGFYAKGLDGRIDDAGAGWRGRAPWSTYATRVP
                     FHIEGGKGTRSRVLKFQLRPDPLAN"
     gene            2800..>3021
                     /locus_tag="VEM33_01135"
     CDS             2800..>3021
                     /locus_tag="VEM33_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50461.1"
                     /translation="MLKVSRVIAVALVAAGLSSVASAQKFDNRRLFFGGGFAQNSVSN
                     FDNATGFQIFGGYNFPELARKFSVDAEAGY"
BASE COUNT          512 a         1020 c          958 g          531 t
ORIGIN      
        1 cggcgaggcc ttggcgagga ctgccggaag atgctccagc acgaaccgcc agacgagctc
       61 gacccgcccg ctgtcctcgc cgatgtgggt gagattgaaa accgcatcgc tcggcggaag
      121 ctcgtcggtc agcagcagct ccatcagccg ctgcagcagc ctcgggtcgc gcacctgtcg
      181 cagcgcggct tgcacctccc acttgtcggt catgtcgacg gcgctgcgca tgcgctcgag
      241 cagggcctcg aaggtcgcct cgtcggcgta gcggccgatg acgttcagca ccgcgggccg
      301 gatcgccgga tcgatgagct tctccgcgcg cgcggcaaag cgggactgcg cttcccgtac
      361 gaccgcttcg tgccccgccc tgccgagcgc ctcgatcagc gacctgcgca gcagcgctgc
      421 gtcggcggct tcgcccggcc gcacgtccca gccggccctc gcaaacggcg cttccagcag
      481 tcgcgcgacg taacgatcga agaccgcctg gtcgcccgga gaatcgatca gctcgcgcag
      541 aaagcgcagt gcgctggtga cctggtccca gatcgtgcgg tcggtctcgg cgccgaggct
      601 ctccacgagc gcgagatagc gcgtcacatc gacgcggccc gcctgcgaga gcgcgaaggt
      661 gtccgagagc aggcgcagac gatccggggc cggaagctcc tgcaaaacga gcaccaggcg
      721 gccgaagttc ccgtcgtcgt actggctccg gtaatagccg gtgtcgcccg cgttcgcgag
      781 catcgcgccg cagcgttcga aacgcagttt ctgcgggttc cgttcgagaa gcaccgtgcg
      841 ccgcgtccct gccgcgtcgg cgaggatcac cggcacattc cacgcgagcg gcgccgctcc
      901 cgggtcgttg agcgtgaagc gctcctgggc gagcgttaca gcggcggagc ctttttcgca
      961 ctcctgcgag actttcacca ccgggtaccc cggctgctcg gtccagcccg cgaccagttt
     1021 gccgatgtct tgccccgatg ctcccgacag atggtgccag aagtcggccg tggtcgtatt
     1081 ggagaagcga tgcgcgcgca tgtagccctg cacgcccgcg cggaaaacgc cttcgccgag
     1141 atagccctcg agcatgcgca gaactgcagc tcctttcgcg taggtgatgg aatcgaagac
     1201 gtccatcgcg cgcgtgtcgt tctcgaccgg cgtctggatc ggatgggtca tcctgcgcgc
     1261 gtcctccgac agggccgact ctttccagag cgcatcgcgc agcggcatgc cccagcgcgg
     1321 gttgaagcgc tccgtggtct tgcccgccat ccacgaggcg aagccctcgt tcagccacaa
     1381 gttgtcccac caggccatcg tgacgaggtt gccgaaccat tggtgcgcga tctcgtgcgc
     1441 gatgatgccg tagaccgctt gctgctggcg cagagaatct tcgcccgggc tgtacaggaa
     1501 gcggccttcg ttgtaggcga tcgcccccca gttttccatc gcgccgctta tgccgcccgg
     1561 aagggcgagc tggtccagct tgaccagagg gtagggttcg ccgaagtagg cgttgaaata
     1621 gcgcacgatc tgcttggtcg cctccatcgc gtatcgcgcg cgctcgcgct tgcctttcac
     1681 ggtatggata ccgagccgga ttccatcgac gctgtcttca aggagatcca tctctccgac
     1741 gaaaagcgcc accagatacg ccgacatcgg caccgacctc gcgaagctca cttccttcct
     1801 gccgtcgggc cggagctcga ccttggactg cggcatgttc gagaccgcgg tcagggtcgc
     1861 gtcgatcacc gtgacgatat cgaaactcgc acggaacacc ggctcgtccc atcccggaaa
     1921 aagccggcgc gcctgcaccg gctccatctg ggtggcgagc atcagcttgt ccacgagccg
     1981 tccgcggtcg cgctccttgt atgcgatctg gtacaggccc tgaggatgct tcccgatctt
     2041 ccccgagtat tcgatgccga gccggtagct gccgggcgca atcggagcgg tcgaaggcgt
     2101 cagcgtcacc gtttcgcgct gcggatcgaa gcgcggcgca aggctcgttt cgtctccagt
     2161 gcccgcacgc agcttcaccg acttgaagct caactcggcc gcattgacga cgatcgccgg
     2221 cacgggccgg gtgacttcga tgtctatgac cgcctcggcg tcgaagcggt cgtaggttgg
     2281 cgcgggaacg atgcgcagcg cgtagtgcct gggcaccacg tccttgggca gcttccccgg
     2341 cgtcgtcgca aacgagaaac gcggctcggc gcccgccgaa gcgatgcagc agagcagcag
     2401 cgccgccgcg agggcaagcg agccggccgc ggttcgatac acggttcgca aggagcgagt
     2461 cgccttcagt tcgcgagcgg atccgggcgc agctggaact tcaggaccct ggaccgggtg
     2521 cctttgccgc cctcgatgtg gaagggcacc cgcgtggcat aagtcgacca cggtgccctc
     2581 cccctccagc cggcgcccgc gtcgtcgatg cggccatcga gcccctttgc gtaaaagccc
     2641 atggaaatgc cgctgatcac ccggttcatc cgcatgcttc tgctcctcgc gtccatccgt
     2701 gcgaaattgg acaattatgc cgccaaaacg catggcggga aacccgaact cggactacca
     2761 ttcgcccgcg gttatacgta gtgagaaagg aggagtgaca tgttaaaagt atcgcgcgtc
     2821 atagcggttg cactcgttgc ggcagggctc tcttccgtcg cctcggccca gaagttcgac
     2881 aacaggagac tgttcttcgg cggcggtttt gcccagaaca gcgtttcgaa cttcgacaac
     2941 gctacgggct tccagatttt cggcggctac aatttccccg aactggcgcg gaagttcagt
     3001 gtcgacgccg aagccggcta c
//
LOCUS       DASPZD010000060         4190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_210255, whole genome shotgun sequence.
ACCESSION   DASPZD010000060 DASPZD010000000
VERSION     DASPZD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210255"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2400)
                     /locus_tag="VEM33_01140"
     CDS             complement(<1..2400)
                     /locus_tag="VEM33_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYS50462.1"
                     /translation="MSRHAVRRSLLLAPLSQHKGRLALSVLAIALGVALGYAVQLINQ
                     AAINEFAAAVQTLSGEADLAVRGPRAGFDESLYPRLAKLPEVAVASPVLEVEARLPGR
                     REPLRILGLDLFRAARIQPFLSSGDWGGRLDFLRPDRIFLSAAASESLGLKKGDSLSV
                     QVGLRMLPLQVAGVLGEAGRQRFAVVDIGAAQAMFLRPGFLNRIDLRLRPGADAERLA
                     ERLRAEMPPGTLIERPDADVERSGALSRAYRVNLNVLALVALFTGGLLVFSAQALAVV
                     RRRAQLALLRVLGMMRRELVAMLLAEAAIVGAMGALAGIALGHASAAFVLERFGAELG
                     GGYFRGLAPAPVLDWPALALFFSLGIFAAVLGSLAPALEAAGAEPAPALKAGDEERAF
                     RRLQPVWPGLAVLLAGAALTQAGPVSGLPLFGYGAIALLLIGTVMLMPRIAVLGFRVA
                     PLLGPVSARIGLAQLRGAPGQAGVSLAAIVASVSLMVSMAIMVASFRQSLDEWLERVL
                     PADLYVRAGPGSDTAFLSPADQQAIATLPGVRRAEFLRSQRLLVAPDRPPLTLLARAV
                     EAEGRVPPLLGSAHALKAGEPPAAWVSEVASDLYGWRAGAVIEIPLGGRRARFTVAGI
                     WRDYARQQGAVLIERYEYLRHTGDAHANDAALWLAPSADAASVTSEIRARVAGGANLE
                     IAGPGEIREVSLNIFDRTFAVTYALEAVAVAIGLFGLSSSFGALVLARRREFGVLRHI
                     GMTRRQVGAMLASEGLAVSGIGLAVGLGLGSAISLILIHVVNRQSFHWGMGVSVPWAT
                     LA"
     gene            complement(2436..2876)
                     /locus_tag="VEM33_01145"
     CDS             complement(2436..2876)
                     /locus_tag="VEM33_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS50463.1"
                     /translation="MEKTIMLVEDNANDEELTLRALRKANIANNVIVARDGQEAVDFL
                     FGTGKHAGREPPVMPAVVLLDLKLPKLDGIDVLHRIRADPRTKLIPVVILTSSSEDED
                     MLRSYQSGANSYVRKPIDFSAFAGAVTQLGMYWILINQSPPTRG"
     gene            complement(2902..>4190)
                     /locus_tag="VEM33_01150"
     CDS             complement(2902..>4190)
                     /locus_tag="VEM33_01150"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HYS50464.1"
                     /translation="LPNRELLGDRTLQAVAHARRAARPAALLVLNVDRFKLVNESYSH
                     GTGDTLLRMVADRLKGAVREGDTVARLGGDAFAVLATDLARPDDVLPVVRKIREAMLS
                     PFSLEGRDLHVTLSIGASVYPRDGEEFDMLLRNADAAMHRVKADGRNGFQFYASAMTR
                     QAADRVELENELRLALERNQLEMHYQPQVVLANGRIVGVEALMRWNHAQRGWIPPGQF
                     VPVAEDSDLIHPLGEFALAESSRQIRAWGDAALAELRLAVNVSARQFRSPGFVKAVER
                     ALRAASLEPRNLELELTEGVLVESRDRTVAVLHGLKELGVQIAVDDFGTGYSSLSYLS
                     RLPIDCLKIDRTFVSQAHRHGQDAAITQAVISLAHSLGVRVIAEGVETVEQLEFLRMH
                     RCDQAQGYLFSPAVHPEAMAQLLAAGSIEPSAGKLN"
BASE COUNT          682 a         1487 c         1461 g          560 t
ORIGIN      
        1 ggcgagcgtc gcccacggaa ccgagacgcc catgccccaa tggaacgatt gccggttgac
       61 cacgtgaatg aggataagac tgatggccga gcccaaaccc aacccgacgg cgaggccgat
      121 gccgctgacg gccagccctt cgctggcaag cattgcgccg acctgtcgcc gcgtcatgcc
      181 gatgtgccgc agcacgccga actcgcgccg ccgcgccagc accagcgcgc cgaagctcga
      241 cgagagcccg aagaggccga tcgcgaccgc caccgcctcg agcgcatagg tcaccgcgaa
      301 ggtgcggtcg aaaatgttga gcgagacctc gcggatctct ccggggccgg cgatctcgag
      361 gttcgcgccg cccgcgacgc gcgcccggat ttcgctcgtc acgctcgccg cgtccgcgct
      421 cggcgccagc cacagcgccg catcgttagc gtgggcgtca ccggtgtggc gcaggtactc
      481 gtagcgctcg atcagcaccg cgccctgctg gcgcgcgtaa tcgcgccaga tgcccgcaac
      541 ggtgaagcgc gcgcgccgcc cgccgagcgg gatctcgatg acggcgccgg ctcgccatcc
      601 gtaaaggtcg gaggcaacct cgctcaccca tgccgccggc ggctcgcccg ccttgagcgc
      661 atgcgcgctg ccgaggagcg gcggcaccct gccctcggcc tcgaccgcgc gggcaaggag
      721 cgtgagaggc ggcctgtccg gagcgaccag caggcgctgg ctgcgcagga actcggcgcg
      781 gcggaccccg gggagcgtcg cgatcgcctg ctggtcggcg ggggagagaa aggccgtgtc
      841 cgagcccggt cccgcgcgca cgtagagatc cgcgggcagc acgcgctcca gccactcgtc
      901 gagcgactgg cggaaggagg cgaccatgat cgccatcgat accatgaggc tcacgctcgc
      961 gacgatcgcg gcgaggctca cccccgcctg tcccggtgca ccgcgaagct gcgcgagccc
     1021 gatgcgcgcc gagaccggcc cgaggagcgg cgctacgcgg aaccctagga ccgcgatgcg
     1081 cggcatcagc atcacggtgc cgatcaagag gagcgcgatg gcgccatagc cgaacaaggg
     1141 cagcccgctc accggccccg cctgagtgag cgccgctccg gcgagaagca ccgcgagtcc
     1201 cggccacacc ggctgcagcc gccggaacgc gcgctcctca tcgcccgcct tcaaggccgg
     1261 cgcgggctcc gcgccggccg cttccagggc gggcgcgagg ctgccgagca ccgctgcgaa
     1321 gatcccgagc gagaagaaca gcgcgagcgc cggccagtcg agcacgggcg caggcgcgag
     1381 gccccggaaa tacccgcccc cgagctcggc gccgaagcgc tcgagcacga aggcggcgct
     1441 cgcatggccg agcgcgattc cggcgagcgc ccccatcgcg ccgacaatgg ccgcttcggc
     1501 gagcagcatc gcaaccagct cccgccgcat catgcccagc acgcgaagca gcgcgagctg
     1561 cgcgcgcctg cgcaccacgg cgagagcctg cgccgaaaaa acgagcagcc ccccggtaaa
     1621 aagcgccacc agcgcgagca cgttgaggtt gacgcggtag gcccgcgaca acgcgccgct
     1681 gcgctcgacg tcggcgtccg gccgctcgat cagagtgccc ggcggcatct cggcgcgcag
     1741 gcgctcggcc aggcgctcag cgtcggcgcc cgggcgcagc cgcaggtcga tgcggttcag
     1801 aaagcccggg cgcaggaaca tcgcctgcgc cgcgccgatg tcgacgaccg caaatcgctg
     1861 acggccggcc tcgcccagca ccccggcgac ctgcagcgga agcatccgca ggcccacctg
     1921 gacggaaagg gagtcgcctt ttttcaggcc gagcgactcg gaggccgcgg ccgagagaaa
     1981 aatccggtcc gggcgcagaa aatccagccg cccgccccaa tccccggacg agaggaaggg
     2041 ctggatgcgg gcggcgcgaa agaggtcgag ccccaggatc ctcagcggct cgcggcgccc
     2101 ggggagccgc gcttccactt cgagcaccgg actcgccacc gccacttcgg gcagcttcgc
     2161 caggcgcggg tacagagatt cgtcgaaacc cgcgcggggg ccgcgcaccg cgagatcggc
     2221 ctctcccgag agcgtctgca cggcggcggc gaattcgttg atggccgcct ggttgatgag
     2281 ctggactgcg tagccaaggg cgacgccgag cgcgatggcg agcaccgaca gggcgagcct
     2341 gcccttgtgc tgcgagagcg gcgcgagcag gaggctgcgg cggaccgcgt ggcggctcat
     2401 tgcgccatgt tagcgcgtgc cgcccgcgcg cgggatcagc cgcgagtcgg agggctctgg
     2461 ttgatcagga tccagtacat cccgagctgg gtgaccgcgc ccgcgaaggc ggagaagtcg
     2521 atcggcttgc gcacgtagct gttcgcgccg gactggtagc tgcgcagcat gtcttcgtcc
     2581 tcgctcgatg acgttaggat cacgacgggg ataagcttgg tgcgcggatc ggcgcgtatc
     2641 cggtgcagca cgtcgatgcc gtcgagcttc ggaagcttga gatcgagcag caccacggcg
     2701 ggcatcaccg gcggctcgcg ccccgcgtgc ttgcccgtgc cgaacaggaa gtcgaccgct
     2761 tcctggccgt ccctcgcgac gataacgttg ttggcgatgt tggccttgcg cagagcgcgc
     2821 agcgtcagct cctcgtcgtt cgcgttgtcc tcgacgagca tgatcgtttt ttccatctcc
     2881 cctcctctgc gccgggcgcc ttcagttgag tttccctgcg gacggctcga tcgagccggc
     2941 agccaggagc tgcgccatcg cctcgggatg aacggcgggg gaaaagagat acccctgggc
     3001 ctgatcgcaa cggtgcatgc gcaggaattc cagctgctcc acggtctcga ctccctcggc
     3061 gatcacccgc accccgagcg aatgcgcgag cgagatcacc gcctgggtga tcgccgcgtc
     3121 ctgcccgtgc cggtgggcct ggctcacgaa cgtccggtcg atcttaaggc aatcgatcgg
     3181 caagcgcgag aggtagctca ggctcgaata gcctgttccg aaatcgtcga cggcgatctg
     3241 cactcccagc tccttcaagc cgtggaggac ggcgacggtc cggtcgcggc tctcgaccag
     3301 cacgccctcg gtgagctcga gctcgagatt gcgcggctcg aggctagccg cgcgcagggc
     3361 gcgctcgacg gccttcacga aaccgggact gcggaactgc cgcgccgaga cgttcacggc
     3421 gagccgcagc tcggcgagcg cggcgtcgcc ccaggcgcgg atctgccggg aactctcggc
     3481 gagcgcgaat tccccgagtg gatggatgag gtcggagtcc tcggccacgg gaacgaattg
     3541 gccgggggga atccatccgc gctgcgcgtg attccagcgc atcagcgctt cgactcccac
     3601 gatgcgcccg ttggctagca cgacctgagg ctggtagtgc atttccagct ggttcctctc
     3661 cagcgcgagc cgcagctcgt tctccaattc gacccggtcg gcggcctgcc gggtcatggc
     3721 ggaggcgtag aactggaagc cgttgcggcc gtcggccttg acgcggtgca tcgcggcatc
     3781 ggcattgcgc aggagcatgt cgaattcctc gccgtcgcgc ggatagacgc tcgccccgat
     3841 gctcagcgtg acgtgcaggt cgcggccctc gagcgagaac ggcgagagca tcgcctcgcg
     3901 gatcttgcgc accacgggca ggacgtcgtc cgggcgggcg agatcggtcg cgagcaccgc
     3961 aaaggcgtct ccgccgagcc gcgccaccgt gtcgccttcg cgcacggcgc ccttcaggcg
     4021 gtcggccacc attcgcagca gcgtgtcgcc ggtgccgtgg ctgtagctct cgttgaccag
     4081 cttgaagcga tcgacgttca ggaccagcag cgccgcgggc cgcgccgcgc gccgggcgtg
     4141 cgcgaccgcc tgcagagtgc ggtcgccgag cagctcgcgg ttcggcaatc
//
LOCUS       DASPZD010000061         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1537434, whole genome shotgun sequence.
ACCESSION   DASPZD010000061 DASPZD010000000
VERSION     DASPZD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537434"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VEM33_01155"
     CDS             <1..1344
                     /locus_tag="VEM33_01155"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYS50465.1"
                     /translation="DAFSAWSETSPLARARVMFRFKELIERHIDELALLVSNEHGKVL
                     SDAKGSVQRGLEVVEFACGIPHLMKGEFSDNISAGMDLYSIRQPLGVCAGITPFNFPA
                     MVPMWMFPMAIACGNTFILKPSEKDPSCPLRLAELMLEAGAPAGVLNIVNGDKEAVDT
                     LLTDPRVAAVSFVGSTPIAKYVFATGSVNGKRVQALGGAKNHMVVMPDADIEAAAEAL
                     MGAAYGSAGERCMAVSVAVPVTERTADELIEAMAPRIRKLKIGPSTDDSSEMGPLVTR
                     EHRDKVKGYVDLGVKEGASLVVDGRGFKAAGQPDGFFLGGSLFDKVRPEMRIYKEEIF
                     GPVLSVVRARNFDEALALPSDHEYGNGVAIFTRNGDVARNFTHKVNCGMVGINVPIPV
                     PLSFHSFGGWKHSIFGDMNQHGPEGVRFYTRIKTVTSRWREGVNPGANFGMPLMK"
     gene            1351..2274
                     /locus_tag="VEM33_01160"
     CDS             1351..2274
                     /locus_tag="VEM33_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS50466.1"
                     /translation="MVDLHDIRYLRIGTPDLQSAVEFATKIVGLQLAGREGKAAYFRS
                     DKTSVRGDTRDHTLVYIEGDPSDHVIGFDLKNREDFDKAGAEIEKAGHPVRYGSKDEC
                     ELRRVKQFLAFEDPTGNKVEIVVRPPHSGERYFPARDAGITHFSHIGLRTSNASRDEA
                     FWTRLLNARVSDWIGDAPLLRISTIHHTLALFPSPHPGVQHINHQVEDFDDVMRSYYF
                     LREKGVRIVFGPGRHPSSSACFLYFEGPDRMVYEYSVGVKHILPEEESSYRPRQFPFA
                     PESFCMWGSRPDIAEFRADQKDKKPPLKTVA"
BASE COUNT          438 a          761 c          764 g          395 t
ORIGIN      
        1 gacgcctttt cggcgtggtc ggagacctcg ccgctcgcgc gggcgcgcgt catgttccgc
       61 ttcaaggagc tgatcgagcg ccacatcgac gagctcgcgc tgcttgtctc gaacgagcac
      121 ggcaaggtgc tctccgacgc caagggatcg gtccagcgcg gcctggaagt ggtcgagttc
      181 gcctgcggca tcccgcatct gatgaagggc gagttctccg acaacatctc cgcgggcatg
      241 gatctgtact cgatccgcca gcctctgggc gtgtgcgcgg gcatcacacc cttcaacttc
      301 cccgcgatgg tgccgatgtg gatgtttccg atggcgatcg cctgcggcaa caccttcatc
      361 ctcaagccct cggagaagga tccgtcctgc cccttgcgcc ttgccgagct gatgctcgag
      421 gcgggcgcgc cggcgggggt gctgaacatc gtaaacggcg acaaggaggc ggtcgacacg
      481 ctgctcaccg acccgcgcgt cgccgcggtc agcttcgtcg gctcgacgcc gatagccaaa
      541 tacgtgtttg ccaccggctc ggtcaacggc aagcgcgtgc aggcgctggg cggagcgaag
      601 aaccacatgg tggtgatgcc cgatgcggac atcgaggccg cggccgaggc gctgatgggc
      661 gcggcctacg gctcggcggg cgagcgctgc atggcggtct cggtggccgt gcccgtgacc
      721 gagcgcaccg cggacgaatt gatcgaagcg atggcgccca ggatcaggaa gctcaagatc
      781 gggccctcga cagacgattc ctcggagatg gggccgctcg tcacgcgcga gcaccgcgac
      841 aaggtgaagg gctacgtcga cctcggagtg aaggaaggcg cgagcctcgt ggtggacggg
      901 cgcggcttca aggcggccgg gcagccggac gggttctttc tcggcggcag cctcttcgac
      961 aaggtcaggc ccgagatgcg catctacaag gaggagatct tcggcccggt gctgtcagtg
     1021 gtacgggcaa ggaacttcga cgaagcgctc gcgcttccct ccgaccacga atacggcaac
     1081 ggcgtggcga tcttcacccg caacggagac gtggcgcgca acttcaccca caaggtaaac
     1141 tgcgggatgg tcggcatcaa cgtcccgatc ccggtaccgc tctccttcca cagcttcggc
     1201 ggctggaagc actcgatctt cggcgacatg aaccagcacg ggccggaagg cgtgcgcttc
     1261 tacacgcgca tcaagaccgt gacctcgcgc tggcgcgagg gcgtgaatcc cggcgcgaat
     1321 ttcggcatgc cgctgatgaa ataacgaacg atggtcgacc tccatgacat ccgctacctg
     1381 cggatcggca cgcccgacct gcagtcagcc gtggagttcg cgaccaagat cgtcggcctg
     1441 cagctcgcgg gccgggaggg caaagccgcc tatttccgca gcgacaagac atcggtgcgc
     1501 ggcgacacgc gcgaccacac gctggtctat atcgagggcg acccttccga ccacgtcatc
     1561 gggttcgatc taaaaaaccg cgaggacttc gacaaggccg gcgcagagat cgagaaggcc
     1621 ggccacccgg tgcgctacgg cagcaaggac gagtgcgagt tgcgccgcgt caagcaattc
     1681 ctcgccttcg aggatccgac cggcaacaag gtcgagatcg tcgtgcgccc gcctcacagc
     1741 ggcgaacgct attttcccgc acgcgacgcc ggaatcacgc acttcagcca catcgggctg
     1801 cgcacctcga acgcttcgcg cgacgaagcg ttctggacgc ggttgctgaa cgcccgcgtg
     1861 tcggactgga tcggcgatgc gccgctcctt cggataagca ccattcacca tacgctcgcg
     1921 ctgttcccgt cgccgcaccc cggggtgcag cacatcaacc accaggtcga ggacttcgac
     1981 gacgtgatgc gctcgtacta cttcctgcgc gagaagggcg tgaggatcgt gttcggccca
     2041 ggccgccatc cttcatcgtc ggcctgtttt ctttacttcg aagggccgga caggatggtc
     2101 tacgagtact ccgttggcgt gaagcacatc ctccccgagg aagaatcgag ctaccggccg
     2161 cggcagttcc cgttcgcgcc cgagtcgttc tgcatgtggg gctcgcggcc ggacattgcg
     2221 gagttccgcg cggaccagaa ggataaaaaa ccgccgctga agaccgtggc ctgaatcgca
     2281 agtctggagt ccgctcttcc gccgaagatg gagaacacaa gaatggaatt tctcgtacag
     2341 tcgtgtccgg gctgatcg
//
LOCUS       DASPZD010000062         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_340183, whole genome shotgun sequence.
ACCESSION   DASPZD010000062 DASPZD010000000
VERSION     DASPZD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340183"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /gene="eno"
                     /locus_tag="VEM33_01165"
     CDS             complement(<1..142)
                     /gene="eno"
                     /locus_tag="VEM33_01165"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYS50467.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLIESGVMGRAAVPSGASTG
                     KRE"
     gene            complement(143..1000)
                     /gene="kdsA"
                     /locus_tag="VEM33_01170"
     CDS             complement(143..1000)
                     /gene="kdsA"
                     /locus_tag="VEM33_01170"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HYS50468.1"
                     /translation="MKLCGFEVGLDKPLFLIAGPCVVESRQMALDTASSLKEICKELG
                     VPFIYKSSFDKANRSSGESYRGLGMDKGLEILAEVKKKIGVPVLTDVHDKEDVAEVAA
                     AVDVLQTPAFLCRQTDFIRAVAAAGRPVNIKKGQFLSPAEMKNVVDKAREASGKDNIL
                     VCERGASFGYNNLVSDMRSLAIMRETGCPVVFDATHSVQLPGGQGTSSGGQREFVPVL
                     ARAAVASGIAGVFMETHPDPDKALSDGPNAWPLDRMKELLATLKELDALVKRGRFAEQ
                     KLKTQKKGA"
     gene            complement(1005..2657)
                     /locus_tag="VEM33_01175"
     CDS             complement(1005..2657)
                     /locus_tag="VEM33_01175"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYS50469.1"
                     /translation="MAKYVFVSGGVVSSLGKGIAAASLAAILESRGIKVTLLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFQSARMHRNNNFTTGQIYDSVIRKE
                     RRGDYLGRTVQVIPHITDEIKAFIARGAGEAEVAIVEIGGTVGDIESLPFLEAVRQMG
                     LELGAHNTCYVHLTLVPFIKSAGEIKTKPTQHSVKELRGIGIQPDALLCRSDRPIPED
                     EKKKIALFTNVQANAVISVWDADSIYRIPWMLHQQMLDEIVCHKLGIIAKPADLRVWK
                     RLVEALEHPQHEITIAMVGKYVDLTDSYKSLNEALVHAGIHARSRVNIRYVDSETIEK
                     DGIGALLKGVDAILVPGGFGKRGVEGMMKAIQYARENKVPYLGICLGMQLAVNEFARN
                     VVGLAGANSTEFDRDTPHPVIALVTEWQDRDGRIERRDEKSDLGGTMRLGAQGAALEP
                     GSLARKVYGADVINERHRHRYEVNNQYVQRLKDKGLRVSGLTQREQLCEMIELPSHPW
                     FIGCQFHPEFTSTPRGGHPLFTAFVEAALQYQASKTKTAIAA"
     gene            complement(2709..>4663)
                     /locus_tag="VEM33_01180"
     CDS             complement(2709..>4663)
                     /locus_tag="VEM33_01180"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HYS50470.1"
                     /translation="TPSPERIEEANLTRFARQAIRDWKLGLNDYPAFYRWTIDHPEQF
                     WDSLWKFASVRASTKGARVLVNGERLPGARWYPQARLNFAENLLRQRDASTAMVFWGE
                     DKVKRRLTHRELYEQVSRVAQGLRASGVKPGDRVAAYLPNLPEAVAAMLAATSIGATF
                     STCSPDFGVQGVLDRFGQIAPAVLFAADGYYYNGKQIDSLQKVREIAARLPGLRRVVV
                     VPYVNDRPDVSAIPGAQRLEDFIADRPANDIRFEQLPFDHPLYILFSSGTTGVPKCIV
                     HGAGGALLKHLCEQQLHCDVKPGDRLFYFTTLGWMMWNWLASALASGATLLLYDGSPF
                     VKDGNVLFDYADAEGMTHLGTSAKFIDAISKAGLEPIRTHRLEHVRTVLSTGSPLAPE
                     GFDYVYRAIKRDVCLSSISGGTDICGCFVLGNPILPVWRGEIQCRALGLKVEVFDENA
                     RSLKQQKGELVCTRPFPSLPVGFWNDPDGTRYRAAYFERFPGVWTHGDWCEITAREGM
                     IIYGRSDTVLNPGGVRIGTAEIYRQVEQLEEVVESLVIGQTWPPGEIGDVRVVLFVRL
                     REGLALDDPLIAKIKNHIRTNTTPRHVPAKVVQVPDIPRTKSNKIVELAVRSVVHGQP
                     VKNLEALANPEALAHFRNRPELAQPD"
BASE COUNT          798 a         1511 c         1468 g          886 t
ORIGIN      
        1 cttcgcgctt cccggtcgac gcccccgaag ggacggcggc gcggcccatg accccggact
       61 cgatcagcac atcggcctcc acagtcgggt tgccccgtga gtcgagaatc tcccttgcga
      121 ccacgtcgac gatcgagctc atttatgcgc cctttttctg cgttttcagt ttctgctccg
      181 cgaagcgccc gcgcttcacc agcgcgtcga gttccttcaa ggttgccagc aactccttca
      241 tccggtccag gggccaagcg ttggggccgt cagagagcgc cttgtccgga tccggatggg
      301 tctccatgaa cacgccggcg atgccgctcg ccaccgccgc acgggccagc acgggcacga
      361 actcgcgctg tcctccggaa gaagtcccct gcccgcccgg gagctgcacc gaatgcgtgg
      421 cgtcgaacac aaccggacaa ccggtctcgc gcatgatcgc gagcgagcgc atgtccgata
      481 cgaggttgtt atagccgaac gaggcgccgc gctcgcacac gaggatgttg tcctttccgc
      541 tcgcctcgcg cgctttgtcc accacgttct tcatttcggc cggcgacagg aactggccct
      601 tcttgatgtt gaccggcctt cccgccgcag cgacggcgcg tatgaaatcg gtctggcggc
      661 agagaaacgc cggggtctgc aggacgtcga cagcggccgc gacttccgca acgtcttcct
      721 tgtcgtggac atcagtcagc accggcacgc cgatcttctt cttcacctct gcgaggatct
      781 ccaagccctt gtccatcccg aggccgcggt acgactctcc cgagctgcgg ttggccttgt
      841 cgaaggagga cttgtagatg aacggcacac cgagctcttt gcagatttcc ttcagcgagg
      901 acgcagtatc gagcgccatc tggcgcgatt cgaccacgca cggaccggcg atcaggaaca
      961 agggcttgtc gaggccgact tcgaagccgc aaagcttcat gctctcaagc cgcgatcgcg
     1021 gtcttggtct tgctggcctg gtactgcaga gcggcctcga cgaacgcggt aaagagcgga
     1081 tggcccccgc ggggtgtcga ggtgaattcc ggatggaact ggcagccgat gaaccaggga
     1141 tgcgatggca gctcgatcat ctcgcacagc tgttcgcgct gggtgaggcc ggaaacgcgc
     1201 aagcccttgt ccttcagccg ctgcacgtac tggttgttga cttcataacg gtggcggtgg
     1261 cgttcgttga tcacgtccgc gccgtagacc ttgcgcgcga ggctcccggg ctcgagcgca
     1321 gcgccctggg cgccaagccg catcgtcccg ccgagatcgg atttttcgtc ccggcgctca
     1381 atgcggccgt cgcggtcctg ccattcggtc acgagcgcaa tcaccgggtg cggcgtgtcg
     1441 cggtcgaatt ccgtgctgtt cgcgcccgcc agcccgacga cgttgcgcgc gaactcgttc
     1501 accgcgagct gcatgcccag gcagatgccg agatacggga ccttgttctc gcgcgcgtac
     1561 tggatcgctt tcatcatgcc ttccacgccg cgcttgccga aaccgcccgg aacgagtatc
     1621 gcgtccacgc ctttcaggag cgcgccgatt ccgtccttct cgatcgtctc cgaatccacg
     1681 taacggatgt tgactcggct gcgcgcgtgg atcccggcgt ggacgagcgc ctcgttcaac
     1741 gacttgtagg aatcggtaag gtcgacatac ttgccgacca tggcgatggt gatctcgtgc
     1801 tgcggatgct cgagcgcctc gaccagcctt ttccacaccc tgagatcggc cggcttggcg
     1861 atgatgccga gcttgtggca gacgatttca tcgagcatct gctggtgcag catccagggg
     1921 atcctgtaga tcgagtccgc gtcccagacg gagatgacgg cattggcctg aacgttcgta
     1981 aacagcgcga tttttttctt ttcgtcctcg ggaatgggcc gatcggagcg gcacaggagt
     2041 gcatcgggct ggatgccgat cccgcgcagc tccttcaccg agtgctgcgt gggcttggtc
     2101 ttgatctcgc ccgcgctctt gatgaaaggc accagagtca agtggacgta gcaggtgttg
     2161 tgcgcgccga gctccaggcc catctgccgc accgcttcga ggaagggcag cgactcgatg
     2221 tccccgaccg ttccgccgat ctcgacgatc gccacttccg cttcgcctgc gccgcgtgca
     2281 atgaacgcct tgatctcgtc ggtgatatgc gggatcacct gcacggtgcg gccgaggtag
     2341 tcgccgcggc gctcttttct aatcaccgaa tcgtagatct ggccggttgt gaagttgttg
     2401 ttgcggtgca tccgcgccga ctggaaccgc tcgtagtggc ccagatcgag gtcggtctcg
     2461 gcgccgtcct cggtgacgaa cacctccccg tgctggaaag ggctcatcgt gccgggatcg
     2521 acgttgatat acgggtcgag cttgaggagg gtgactttga tgccgcgcga ttcgaggatc
     2581 gcggcgaggg aggcggcggc tatccccttc cccagagagg aaaccacgcc gcccgacacg
     2641 aacacatact tggccatcta aaacccgggg gatgggatgg agaatggtac atgaaaaccc
     2701 ctccccgctc aatccggctg ggcgagctcc ggtcgattgc ggaaatgcgc gagcgcctcg
     2761 ggattcgcga gggcctcgag gttcttcacc ggttgcccgt gcacgacgct gcgcacggcg
     2821 agctcgacga tcttgttcga cttggtgcga ggaatgtcag gcacctgcac gaccttcgcg
     2881 ggcacatgcc gtggagtggt gttcgtgcgg atgtggttct tgattttcgc gatcagggga
     2941 tcgtccagcg cgagaccttc gcgcaacctc acgaacagca ccacgcgcac gtcgccgatc
     3001 tctcccggcg gccaggtctg gccgatcacg agcgactcga ccacctcctc cagctgctcg
     3061 acctgtcggt agatctcggc cgtgccgatg cgcacgcctc ccggattgag caccgtatcc
     3121 gatctgccgt agatgatcat gccttcgcga gcggtgatct cgcaccagtc gccgtgggtc
     3181 cagaccccgg gaaatctctc gaagtaggcg gctcgatatc tcgttccgtc ggggtcgttc
     3241 cagaatccga ccggcagcga cggaaacggc cgcgtacaga cgagctcgcc cttttgctgt
     3301 ttcagcgagc gcgcgttttc gtcgaacacc tcgaccttga ggccgagggc ccggcactgg
     3361 atttcgccgc gccacaccgg caggatcggg ttgccgagca cgaagcatcc gcagatgtcg
     3421 gtgccgcccg agatcgacga gaggcacacg tcgcgcttga tcgccctgta gacgtagtcg
     3481 aaaccctcgg gggcgagcgg ggagcctgtc gaaagcacgg tgcgcacatg ctcgagccgg
     3541 tgcgtcctga tcggctccaa tcccgccttg gagatcgcgt cgataaattt cgcggaagtg
     3601 ccgagatgcg tcattccctc ggcatcggcg tagtcgaaaa gaacgttgcc gtccttgacg
     3661 aacggcgagc cgtcgtagag cagcagcgtc gcgcccgaag cgagcgccga ggcgagccag
     3721 ttccacatca tccagccgag cgtggtgaag tagaacagcc gatctccagg tttcacgtcg
     3781 caatgcagct gctgctcgca gagatgcttg agcagcgcgc cgccggctcc atgcacgatg
     3841 cacttgggca ctccggtcgt tcccgaggaa aacaggatgt acagcggatg gtcgaagggc
     3901 agctgctcga agcggatgtc gttcgccggc cgatcggcga tgaagtcctc cagacgctgc
     3961 gcgcccggaa tggcggaaac gtccggccga tcgttcacgt agggaacgac gacgacgcgt
     4021 ctcaggcccg gcagccgcgc agcgatctcc cgcactttct gcagcgagtc gatttgcttt
     4081 ccgttgtagt agtacccgtc ggccgcgaag agcaccgccg gggcgatctg gccgaagcgg
     4141 tcgagcacgc cctggacccc gaaatcgggg gaacacgtcg agaaggtcgc gccgatgctc
     4201 gtcgcggcga gcatcgcggc gaccgcttcg ggcaggttgg gcagataagc ggcgacccgg
     4261 tcgccgggtt tcacgcccga ggcgcgcagt ccctgtgcga ctcgcgagac ttgctcgtac
     4321 agctcgcgat gcgtgaggcg tcgcttgacc ttgtcttcgc cccagaacac catcgccgtg
     4381 gacgcgtcgc gctgccgcag caggttctcc gcgaagttga gacgcgcctg cggataccag
     4441 cgcgcgcccg gcaaccgttc gccgttcacg agcacccgcg cgcccttcgt gctcgcgcgc
     4501 acgctcgcga acttccacag cgaatcccag aactgctccg ggtgatctat cgtccagcgg
     4561 tagaaggccg gatagtcgtt caatccgagc ttccagtcgc ggatagcctg acgcgcgaaa
     4621 cgcgtgaggt tcgcttcttc gatgcgctcg ggactcggcg tcc
//
LOCUS       DASPZD010000063         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_90112, whole genome shotgun sequence.
ACCESSION   DASPZD010000063 DASPZD010000000
VERSION     DASPZD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90112"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VEM33_01185"
     CDS             <1..1364
                     /locus_tag="VEM33_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HYS50471.1"
                     /translation="GNPVRLLRNSRIGAYVYPVVPSEFSNWRDEQSAWRKTAVLFDQS
                     HHMAEITVKGPDALKLMSYCTINSFANFTPNRAKQMVPCSHDGCVIGDGIVFYLDKGE
                     LLFVGRTPSVNWIQFQVETGGFKVDYIRDDRSPSHPRGKAVIRRHYRLQIQGPNAAAI
                     LEKINGGPLPEIEFFHLGVINIAGRKVRALRHGMAGAPGLEMWGPYAEGDQIRDAVLE
                     AGREFDLVQVGSRAYASNTLESGWIPSPLPAVYTGEKMKKYREWLPANGYEGTGSIGG
                     SFVSDSIEDYYVTPYELGYGPFVKFDHDFIGREALQKKSEGPHRRKVTFEWNGQDLAG
                     ILASLLVPGAEPCKFFDLPIANYASSSYDKVTKDGRTVGFSMFGGYSYNERAGLSLGI
                     VDPDVHIGDTLTLVWGEEGGGTRKTTVERHRPVAVRVKVAPVPYAREARETYHQGWRT
                     RQV"
     gene            1379..1669
                     /locus_tag="VEM33_01190"
     CDS             1379..1669
                     /locus_tag="VEM33_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50472.1"
                     /translation="MDKFSNQTFSGSWIVIDGHEYVNCRFEKCKIVYCGASSVVLNGC
                     LFDDCEWTFDGPAARTVQFMTALYAMGGGAQQLIEQTFDNIRGKPHPSVKPN"
     gene            complement(1687..>2067)
                     /locus_tag="VEM33_01195"
     CDS             complement(1687..>2067)
                     /locus_tag="VEM33_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50473.1"
                     /translation="LAAQAWAAHGRGERAEALKQIRAAADLEDRSEKQVTMENRLYPM
                     RELLGDMLRENGQGELALREYEASNKATPRRLRGLYGAAKAAQMSGQADKAASYFRDL
                     ARLTAHADTDRPELTEARQFLARR"
BASE COUNT          373 a          670 c          665 g          359 t
ORIGIN      
        1 ccggcaaccc ggtcaggctg ttgcgcaact cgcgcattgg agcctacgtc tacccggtcg
       61 tgcccagcga gttcagcaat tggcgcgacg agcagagcgc atggcgcaag acggcggtgc
      121 tgttcgacca gtcgcaccac atggcggaaa tcaccgtcaa ggggcccgat gcgctcaagc
      181 tgatgtcgta ttgcacgatc aacagcttcg ccaacttcac gccgaacagg gccaagcaga
      241 tggtgccctg cagtcacgat ggctgcgtga tcggcgacgg gatcgtgttc tacctggaca
      301 agggcgagct gctgttcgtc ggccgcacgc cgtccgtgaa ctggatccag ttccaggtcg
      361 agacaggagg cttcaaagtc gactacatcc gcgacgaccg ctcgccctcg cacccgcgcg
      421 gcaaggccgt gatccggcgc cactaccgat tgcagatcca ggggccgaac gccgccgcga
      481 tcctggagaa gatcaacggc ggcccgctcc cggagatcga gttcttccac ctcggcgtga
      541 tcaacatcgc gggccgcaag gtgcgcgcgc tccgacacgg tatggccggc gcgccaggcc
      601 tggagatgtg gggtccgtac gccgagggcg accagatccg cgacgccgtt ctcgaggccg
      661 gccgggaatt cgacctcgtc caggtcggct cgcgcgcgta tgcgagcaat accctcgagt
      721 cgggctggat cccgtcgccg cttccggccg tatacaccgg cgagaagatg aagaaatacc
      781 gcgagtggct gccggcgaac ggttacgagg gcacgggttc gatcggcggc agcttcgttt
      841 cggacagcat cgaagactac tatgtgacgc cgtacgagct ggggtacggc cccttcgtga
      901 agttcgacca cgacttcatc ggccgcgagg cgctccagaa gaagtccgag gggccacatc
      961 gcaggaaggt gaccttcgag tggaacggcc aggaccttgc cggcatcctg gcttccctgt
     1021 tggtgccggg cgccgagccc tgcaagttct tcgacctgcc gatcgcgaac tacgcctctt
     1081 cctcgtacga caaggtgacg aaggacggca ggacggtcgg cttctcgatg ttcggcggct
     1141 acagctacaa cgagcgtgcg ggcctctcgc tgggaatcgt ggacccggac gtccacatcg
     1201 gcgacacact gacactcgtc tggggcgagg aaggcggcgg cacccgcaag accaccgtcg
     1261 aacgtcaccg gcccgtcgct gtgcgggtga aggtagcgcc ggttccctac gcacgcgaag
     1321 cgcgtgagac ctaccatcaa ggctggcgca cccgtcaggt gtgagccggc aggcgctcat
     1381 ggacaagttc agcaatcaga ccttttccgg ttcctggatc gtcatcgacg gccatgaata
     1441 tgtgaattgc cgtttcgaga aatgcaagat cgtctattgc ggagcctctt cagtcgtcct
     1501 caacgggtgc ctgttcgatg actgcgaatg gacattcgat ggaccggccg cgcggacggt
     1561 tcaattcatg acggctttgt acgccatggg cggaggggcc cagcaactga tcgagcagac
     1621 cttcgacaac attcgcggaa agccgcaccc gagcgtgaag cccaactagg ccgggttcgc
     1681 gcccgcctac cggcgcgcaa gaaattgtcg ggcctctgtc aattccggcc gatcggtatc
     1741 ggcgtgggcg gtgagccgcg ccaagtcccg aaagtacgag gccgccttgt cggcctgacc
     1801 ggacatctgc gcggccttgg cggcgccgta caggccgcga agccggcgtg gggtggcctt
     1861 gttggacgcc tcgtactcgc gcagcgcgag ctcgccctgc ccgttttcgc gcagcatgtc
     1921 gcccagcagc tcacgcatcg gatacagccg gttttccatc gtgacctgct tctcgctgcg
     1981 atcctccaga tcggccgccg cgcgtatctg tttaagcgcc tcggcccttt cgccgcgtcc
     2041 atgggccgcc cacgcttgcg cggcgag
//
LOCUS       DASPZD010000064         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_209037, whole genome shotgun sequence.
ACCESSION   DASPZD010000064 DASPZD010000000
VERSION     DASPZD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209037"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..953)
                     /locus_tag="VEM33_01200"
     CDS             complement(81..953)
                     /locus_tag="VEM33_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYS50474.1"
                     /translation="MAASRTLAIAGDNAVPSTLSVRAALLLTLAAVALRAVAADAQDP
                     LAAAIASPSRTPKFTLRDQYRHPLETLRFFGVRPNQTVVEIWPGRGWYMEILAPYLRE
                     QGKYYAAIEAPDVPGAAKEAKDNAAVLRKRIADDPTHLGKVVVTELHPPQLTEICPPG
                     TADVVLTFRNVHNWLASGDQQAQFNAFFKALKPGGVLGVVEHRAKPGTSLEEMRKSGY
                     MDEDYIKKLAGTAGFRFDAESPINNNPKDTKDYPEGVWTLPPTLALGDKDRDKYLAIG
                     ESDRMTLRFVKPGR"
     gene            1088..>2684
                     /locus_tag="VEM33_01205"
     CDS             1088..>2684
                     /locus_tag="VEM33_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS50475.1"
                     /translation="MSLITLLNAHIAFGERPLLDEAQLTVQAGERLGLIGRNGTGKST
                     LLGVLAGKTRLDDGELQRREGLRLGLVEQEPQLPPAETLQDSLLLRATACAAWSEGAP
                     ADEREHWRLESRLEEFLHRFGLTGVISPASCSGGERKRAVLALALALQPDLLLLDEPT
                     NHLDIEAIERLEELLLKVPASTVITHDRAFLDRITTRIIELDRGVLRSYPGNFAAYEQ
                     RKDQELAAEDTARRRFDKFWQQEEAWIRKGVEARRTRNEGRVRRLEQLRIERATRRDR
                     LGNIRFALNAGERSGELVAEFDRVSKRFAARPLIEDLSMRIMRGDRIGLIGPNGAGKS
                     TLIRLILGELAPDAGTIRRGTRLSIAYFDQLRAQLDPTATLAEAISPGSDWVQLGSER
                     KHIVSYLGEFLFPAQRAKSPISMLSGGERNRLLLARLFAQPANLVILDEPTNDLDIES
                     LELLEERLQDYKGTLLLVSHDRRFLDNVVTQTLAAEGNGVWREYAGGYSDYLQQRPPI
                     PVGRASEAGRTPARRASRDHSARK"
BASE COUNT          487 a          877 c          831 g          489 t
ORIGIN      
        1 attttaaggc gcccccgccc accgcgatcc aacccctcag ccgcacactc ttcacggcag
       61 tggttcgtcc cgtggtcggc ttacctgccg ggcttcacga atctcagtgt catccgatcc
      121 gactcgccga tcgccagata cttgtcgcga tccttatcac cgagcgcgag cgtcggaggc
      181 agggtccaca ccccttccgg atagtccttg gtgtccttcg ggttgttgtt gatcggcgat
      241 tccgcatcga agcggaagcc cgcagtcccc gccagtttct tgatgtagtc ctcgtccatg
      301 tagccggact tacgcatctc ctcgagactg gtgcccggct tggcgcggtg ctcgacgaca
      361 ccgagcacgc cacccggctt caaagccttg aagaatgcgt tgaactgcgc ctgctgatcg
      421 ccacttgcta accagttatg cacgttacgg aacgtcagca ccacatcggc tgtcccaggc
      481 gggcagatct cggtaagctg cggcggatgc agttcggtga cgaccacctt gccgaggtga
      541 gtcggatcgt ccgcgatccg cttgcgcagc acggccgcat tgtccttagc ctccttcgct
      601 gcgcccggta catcgggagc ctcgatggcg gcgtagtact tgccctgttc gcgcaggtag
      661 ggggccagga tttccatata ccagccgcgg ccgggccaga tctcgactac ggtctgattc
      721 ggccgcactc caaagaatcg cagcgtttcc agcggatggc gatactggtc gcgcagcgtg
      781 aatttcggag tgcgcgaggg actggcaatc gccgcggcta gcgggtcttg ggcatccgcc
      841 gccaccgctc tgagggcaac ggcagccagc gtcagcagca acgcggcacg aactgatagt
      901 gtggaaggca cggcgttatc tccggcaatg gcgagggttc gcgaagccgc catattactg
      961 ccacccggct ttcgcgaaga cgcgatcgtt ccttcgtggc gcagctgacg tggtcggcgc
     1021 tctgtgccaa ggaacgcccc agcttcgtag actttgccat cgcacgtcca atccggacaa
     1081 tgcgcctatg tccctgatca cacttctcaa tgcgcatatc gccttcggcg agcggccgct
     1141 tcttgatgaa gcgcaactca ccgtacaggc cggcgagcga ctcgggctga tcgggcgcaa
     1201 cggcaccggt aagtcgaccc tgcttggtgt gctcgcgggt aagacgcggc tcgatgacgg
     1261 cgaactgcag cgacgcgagg gcttgaggct tgggctcgtc gaacaggaac cgcagctccc
     1321 cccggcagaa acgctgcaag acagtctgct gctgcgcgcc acggcatgcg cggcctggag
     1381 cgaaggggcg ccagccgatg agcgcgagca ctggcgcctg gagtcgcgcc tcgaggaatt
     1441 tctgcaccgc ttcggtctta ccggcgtcat ttcaccggcg agttgttcgg gcggagagcg
     1501 caagcgtgct gtgcttgccc tggcgctcgc gctacagcct gatctgctgc tgctcgatga
     1561 gcccaccaat catctcgata tcgaagccat cgaacgattg gaggagctgc tgcttaaggt
     1621 gcctgcgtcc acggtcatca cccacgatcg cgccttcctc gatcggatca ccacccgcat
     1681 catcgaactg gaccgcggcg tactgcgctc ctaccccggc aacttcgcgg cctatgagca
     1741 gcgcaaggac caggaacttg cggcggaaga caccgcgcgg cgccgtttcg acaagttctg
     1801 gcagcaggag gaagcctgga tccgcaaagg cgtcgaggct cgccgaacac gcaacgaagg
     1861 acgcgtgcgc cgtctcgagc aattgcgaat cgagcgcgct acgcgacgcg accggctcgg
     1921 aaacattcgc ttcgctctca atgccggtga gcgctcaggc gagctggtgg ccgagtttga
     1981 cagggtgagc aaacgcttcg cagcccgacc gcttatcgaa gatctctcga tgcggatcat
     2041 gcgtggcgat cgcatcggcc tgatcggccc gaacggcgcg ggaaagtcga ccctgatccg
     2101 tctcattctc ggcgaactgg cgccggacgc cggcacgata cgccgcggaa cccgcctaag
     2161 catcgcctac ttcgatcagc tgcgagccca actcgatcca accgcgaccc tcgccgaggc
     2221 catcagtccc ggctcggact gggtgcagct cgggagcgag cgcaagcaca ttgtgagcta
     2281 tctgggggag ttcctgtttc ccgcgcagcg cgcgaagtca ccgatcagca tgctctccgg
     2341 cggcgagcga aatcgtctcc tgctcgcgcg cctgttcgcg caacctgcaa acctagtcat
     2401 attggatgag ccgaccaacg atctcgacat cgaatccctg gagctactcg aggagcggct
     2461 gcaggactac aaggggacgc tgctgctggt gagtcacgac cggcgctttc tcgacaatgt
     2521 cgtcacccaa acgttggccg ccgaggggaa tggggtgtgg cgcgagtacg ccggcggtta
     2581 cagcgactac ctgcagcagc ggccgccgat accggtcggc cgcgcaagtg aggccgggcg
     2641 gacgcctgcg cggcgtgctt cgcgcgacca ctctgcgcgt aaaa
//
LOCUS       DASPZD010000065         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_338826, whole genome shotgun sequence.
ACCESSION   DASPZD010000065 DASPZD010000000
VERSION     DASPZD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338826"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VEM33_01210"
     CDS             <1..690
                     /locus_tag="VEM33_01210"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HYS50476.1"
                     /translation="GNDGTVARCGWKAQNKSLLLFSGEAYNVEMGITNELFQTERDET
                     PSCQFAEVPNTVTDTAGKTAVDAVSPIEKFAFFMRFLAPPAPSRDAPGGAESIARGRK
                     LFAAIGCALCHTPTLKTGNSAVAALRNQPVNLFSDLLVHDMGPGLADGVTQGQAGPRE
                     FRTAPLWGLGQRLFFLHDGRTSDLREAIRAHRSGSFFTRNASEANAVIKNFNGLADAQ
                     KQDVLNFLRSL"
     gene            complement(710..1789)
                     /locus_tag="VEM33_01215"
     CDS             complement(710..1789)
                     /locus_tag="VEM33_01215"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HYS50477.1"
                     /translation="MSALASSAPAATFVYVSNAEDGEIAAYAVQPDATLRALARTQVA
                     KTVMPMAVSPSRRLLYAASRAKPYTVHVYAIDPGTGALKALSTAPLAESFPYISLDRN
                     GRFLFGASYGGHLVSVNLVGGDGRVFPEPLQVIPVGRNAHSILVDATNRFVYVPNLGT
                     DQIFQFLFDEKSGRLASNTPAVAQMKPGTGPRHFVFSRDNRFVYLLSELLATVTTLSL
                     DAKMGLLTEVSSASALPPDSKLAPGSPRPHPRNTDNDIWAADLHLTPNGKFLYASERT
                     SSTLAAFGVDAASGKLSYLSSTPTEKQPRGFAIDPGGRFLVASGEKSETISVYALDPA
                     SGALKLLQKVPAGKGANWVEIVAFD"
     gene            1755..2492
                     /locus_tag="VEM33_01220"
     CDS             1755..2492
                     /locus_tag="VEM33_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50478.1"
                     /translation="MAAGALLASADMAMIPQVKNDFMAVFLCRFANLNRLCPAQARGY
                     SIPKCSWHPHCLLAAGILEMGSIPTFTAIGSPKTDDYRSLMTRHQRQGEIMLTIRQKV
                     SALLGIAMLLGCATLVSAAGDPDKLSDAREMLQRGEVASAVSSLENALPPLMASQDYW
                     SVSKAYFQLAVARGRLNETRAACAALSKSQDYYRQALIKDNLSLDYFGEMASDGSDGS
                     EGLREVRARLGCEGTRTASSNPYRKAK"
     gene            2559..3698
                     /locus_tag="VEM33_01225"
     CDS             2559..3698
                     /locus_tag="VEM33_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50479.1"
                     /translation="MRTSRVSAAFVLAASAAYAACAFAENGVGAKQILIGQSASLTGG
                     AAESGQQTRDGALAYFEVVNRKGGVNGRSIKLLTIDDGGQTKRGEENTRKLIAEDKVF
                     LLFGYTGRNTSEAALPIVAEANIPFFGAATGGETIHGAFNKNVFNVRASYKLETLALV
                     NYLAITGQTKIGMIYHKDDLTKSNLKMTEDAAAQHGLKLVGSASVDRNSADVKEAFGI
                     MSKLNPDAVICNAAVKPLSEFVKQMRTSGAASQFLSVSFVGSAIVKELKSEAAGVIMA
                     QVVPLPTKKGIPIVAEYHNALAAVGAKPDYSFSGLEGFISAKVLVEGLKRSGKAPTRA
                     GFIKAMEGIRELDLGDYFVSYSPTNHNGSKYVDITVINKVGLFFN"
     gene            complement(3708..>3905)
                     /locus_tag="VEM33_01230"
     CDS             complement(3708..>3905)
                     /locus_tag="VEM33_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HYS50480.1"
                     /translation="ALALGAQFVFVGRPFLYAAAIGGEAGVRHGIRLLSEEIERDMAL
                     LGITKLSEMTRERIRPRPQVA"
BASE COUNT          725 a         1220 c         1300 g          660 t
ORIGIN      
        1 ggcaacgacg gcacggtcgc gcgctgcggg tggaaggccc agaacaaatc gctgctcttg
       61 ttctccggcg aggcgtacaa cgtggaaatg gggatcacca acgagctgtt tcagaccgag
      121 cgcgatgaaa ccccgagctg ccagtttgca gaagtcccca acaccgtcac cgacaccgcg
      181 ggcaagacgg ctgtcgacgc cgtcagtccg atcgagaagt tcgccttctt catgcgtttc
      241 ctcgcgccgc cggcgccttc gcgcgatgcg ccgggaggtg ccgaatcgat cgcgaggggc
      301 cggaagctct tcgccgccat cggctgcgcg ctctgccata cgcccacgct caaaacgggc
      361 aattccgccg tcgcggcgct gcgcaatcag ccggtgaatc tcttctccga cctgctcgtc
      421 cacgacatgg gaccggggct ggccgacggg gtcacccagg gacaggcggg ccccagggag
      481 tttcgcaccg ctccgctgtg gggactggga cagcgcctgt tcttcctgca cgacgggcgt
      541 acgtcggacc tgcgcgaggc gatccgggcg catcgcagcg ggagcttctt cacccgcaac
      601 gcctccgaag cgaatgccgt gatcaagaac ttcaacgggc tcgcggacgc ccagaagcag
      661 gacgttctca acttcctgcg ttcgctgtag cggaaggcgg gcggcgactt caatcgaagg
      721 cgacgatctc cacccagttc gcgcctttcc cggccgggac tttctgcagg agcttcagcg
      781 cgccgctcgc ggggtcgagc gcgtagacgg agatcgtttc cgatttctcc ccggaagcga
      841 cgaggaagcg tcccccggga tcgatcgcga acccgcgcgg ctgcttctcc gtcggcgtgc
      901 tcgagagata gctgagcttc ccgcttgccg catcgacgcc gaacgcggcg agcgtgctcg
      961 aggtgcgctc cgaggcgtac aggaattttc cgttcggggt gaggtgcagg tccgccgccc
     1021 agatgtcgtt gtcggtgttg cgcgggtgcg ggcgcggcga gccgggcgcc aatttcgaat
     1081 cgggcggcag ggccgaggcc gagctgactt cggtcagcaa tcccatcttc gcgtcgagcg
     1141 acagcgtggt caccgtggcg aggagctccg agagcaggta tacgaaccgg ttgtcgcggg
     1201 agaagacgaa atggcgcggg ccggtcccgg gcttcatctg cgcgaccgcg ggcgtgttcg
     1261 aggcgaggcg cccggacttc tcgtcgaaga ggaactggaa gatctggtcg gtgccgaggt
     1321 tcgggacgta gacgaagcgg ttggtcgcgt cgacgaggat cgaatgggcg ttgcggccca
     1381 cggggatcac ctgcaggggc tcggggaaga cgcgcccgtc gccgccgacc aggttgacgc
     1441 tcaccaggtg gccgccgtag gacgcgccga agaggaagcg cccgttgcgg tcgagcgaga
     1501 tgtacgggaa gctctcggcc aaaggcgcgg tggacaaggc cttcagcgcg cccgtccccg
     1561 ggtcgatggc atagacgtgc accgtataag gcttcgcgcg cgaggccgcg tagagcaggc
     1621 gccggctcgg actcaccgcc atcggcatca ccgtcttggc cacttgcgtg cgcgcgagcg
     1681 ctctcagcgt cgcgtcgggc tgcaccgcgt acgcggcgat ctcgccgtcc tcggcgttgg
     1741 agacgtagac gaaggtggcg gcgggtgcgc tgcttgccag tgccgacatg gcgatgatcc
     1801 cccaagtcaa gaatgatttc atggcggtct tcctttgtcg ttttgcgaat cttaaccggc
     1861 tttgcccggc gcaggcgcgg gggtattcga tcccaaagtg cagctggcat ccacattgct
     1921 tacttgctgc ggggattctt gaaatgggct cgatcccgac gtttacggcc atcggaagcc
     1981 cgaaaaccga cgactatcga tcacttatga ctcgacatca acgccaagga gaaatcatgc
     2041 taacgatccg tcaaaaggtg tctgcgctcc tgggtatcgc catgttattg ggttgcgcga
     2101 ccctcgtctc ggcggccggc gatccggaca agctctcgga tgcgcgtgaa atgctgcagc
     2161 ggggcgaggt tgcgagcgcc gtgtcttccc ttgagaacgc gctgccgccc ctcatggctt
     2221 cgcaggacta ttggtcggta agcaaggcgt attttcagct cgctgttgcg cgcggccgtc
     2281 tgaacgaaac tcgcgccgcc tgcgccgcac tgtcgaagtc ccaagactac tatcgccagg
     2341 cgctgataaa ggacaacctc tctctggact acttcggcga gatggcgtcc gacggcagcg
     2401 acggcagcga aggattgcgg gaagtgcgcg cgaggctcgg atgcgagggc acccggactg
     2461 cctcgtcgaa tccctacagg aaggcgaaat agggcggttg ggcgtcaagc cgacgacgat
     2521 ctataatcaa cgacgtctct ccaacgagcg atcccaatat gcgtacgtcc agggtttcag
     2581 cggcgttcgt tctcgcagcg agcgccgcgt acgcggcgtg cgcgttcgca gagaacggcg
     2641 tcggcgcgaa gcagatattg atcggccagt ctgcctccct gaccggcggg gcggccgagt
     2701 ccgggcagca gacgcgcgac ggcgcgctcg cctatttcga ggtggtcaac cgcaagggcg
     2761 gcgtcaacgg cagaagcatc aagctgctca ctatcgacga cgggggccag accaagcgcg
     2821 gcgaggaaaa cacgaggaaa ttgatcgcgg aggacaaggt cttcctgctg ttcggctaca
     2881 ccgggcgcaa taccagcgag gccgccctgc cgatcgtcgc ggaggccaac ataccgtttt
     2941 tcggagccgc caccggcggc gaaaccatcc acggcgcgtt caacaagaac gtgttcaacg
     3001 tgcgcgcgag ctacaagctg gagacgctgg ccctggtcaa ctacttggcg atcaccggcc
     3061 agacgaagat cgggatgatc taccacaagg acgatctgac gaagtcgaac ctcaagatga
     3121 ccgaggacgc cgccgcgcag cacggcctta aactggtggg cagcgcttcg gtcgaccgca
     3181 attcggccga cgtgaaggaa gccttcggga tcatgagcaa gctcaatccc gatgcggtga
     3241 tctgcaacgc ggcagtgaaa cccctgagcg aattcgtgaa gcagatgaga acgtcgggcg
     3301 ccgcttcgca gttcctgagc gtctcgttcg tcggcagcgc gatcgtgaag gagctgaaaa
     3361 gcgaggcggc cggggtgatc atggcgcagg tcgtgcccct gccgaccaag aagggcattc
     3421 ccatcgtggc ggagtaccac aacgccctgg cggccgtggg cgccaagccc gactattcgt
     3481 tctccggcct agagggcttc atctcggcca aggtgctggt ggaggggctg aagcggagcg
     3541 gcaaggcccc gacccgcgcc ggattcatca aggccatgga aggaatccgc gagctcgacc
     3601 tgggcgacta tttcgtcagc tactcgccga ccaatcacaa cggctcgaag tacgtcgata
     3661 tcacggtcat caacaaggtc ggcctttttt tcaattgacg cctcgagtca ggcgacctgc
     3721 ggccgcggcc tgatccgctc gcgcgtcatc tccgacagct tggtgatgcc gagcagcgcc
     3781 atatcgcgct cgatttcctc ggagaggagc ctgatgccgt ggcgcacccc ggcctccccg
     3841 ccgatcgctg cggcgtagag gaagggccgg ccgacgaaca cgaactgcgc cccgagcgcg
     3901 agcgc
//
LOCUS       DASPZD010000066         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_207889, whole genome shotgun sequence.
ACCESSION   DASPZD010000066 DASPZD010000000
VERSION     DASPZD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207889"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VEM33_01235"
     CDS             <1..749
                     /locus_tag="VEM33_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50481.1"
                     /translation="LGRECARLFNDWVAKLVKGNENVFLPVAMAPAGCPEAMADELKR
                     CVKQLGFRASHLVPYCGTRNLDDPSFYPYYQAAEELGVPLLCHPNSNGELVDRFDNFY
                     KTHVLGRPTNCSAALVALVLGGVFEKFPGLKVVFFECSAEWILYWMHRMDDDWEWAKD
                     FPQISGMLKMAPSEYIRRNCWVTCEADEADLARPIAELGEDHILMASDYPHFDSEFPH
                     TVAAIRARKDLTERQKDKILGENAAQLLRL"
     gene            724..1779
                     /locus_tag="VEM33_01240"
     CDS             724..1779
                     /locus_tag="VEM33_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS50482.1"
                     /translation="MRLSCFVFSVLLAPPAFGVEPTPGVAAVAAYSGPDRTARLIAGA
                     KKEGELMLYSSLTQDDQLKLVADFKRRYGVTVKFWRGSQAHILQRVTSETRGGRFEFD
                     VLETNAPQIEALAREKLLQKMNSPYVEQELLPEVMPSHGEWAPDRLNLLVYAYNTHLV
                     KPADVPKSWQDLLDPKWKKRIGVESTNVEWFAALVKSLGEKEGLELFRRMADNGLAVR
                     TGHNHSAVLVAAGEIPLMLGIYSHDAERMKAKSAPVDWFVLPPAVVLASAVAVSRRAP
                     RPNAAALFYDYMLTDGQRFYTEVHRVPANKNYDTPVRRLVRERQAVKIVNAQETADEY
                     EKWLDLYKRIVVDRSQH"
     gene            1793..>2241
                     /locus_tag="VEM33_01245"
     CDS             1793..>2241
                     /locus_tag="VEM33_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50483.1"
                     /translation="MKSRSVRLRLYAAAFAALAALGCAAQTVIHERTSPYNTVIVTED
                     YSGMRTLLFERGGGRQSVVKPGDPDHLELPYARVALAGLALCEEPRRILVVGLGGGSL
                     PMFLRAHYPGAMIDVAEIDPEVVDVAKKFFGFVEDERMRARVGDGRQ"
BASE COUNT          406 a          737 c          725 g          373 t
ORIGIN      
        1 gcctcggccg cgagtgtgcg cgccttttca acgattgggt tgcgaagctc gtgaagggca
       61 acgagaacgt attcctgccg gtggcgatgg ccccggcggg atgccccgaa gcgatggccg
      121 acgagctcaa gcgctgcgtg aaacagttgg gctttcgcgc ctcgcacctc gttccctatt
      181 gcggcacgcg caatctcgac gacccgagct tctatcccta ctaccaggcg gccgaggagc
      241 tcggcgttcc gctcttgtgc catcccaaca gcaacggcga gctcgtcgac cgcttcgaca
      301 atttctacaa gacgcacgtg ctcgggcgcc cgaccaactg cagcgcggcc ctggtggcgc
      361 tggtgctggg aggcgtcttc gagaaattcc ccgggctcaa ggtcgtgttc ttcgagtgca
      421 gcgcggagtg gatcctgtac tggatgcatc gcatggacga cgactgggag tgggcgaagg
      481 atttcccgca gatctcgggc atgctgaaga tggcgccctc ggaatacatc cgccgcaact
      541 gctgggtcac ctgcgaggcg gacgaggccg atctcgcgcg gcccatcgcc gagctcggcg
      601 aggatcatat cctcatggcg agcgactatc cgcacttcga ctcggagttt ccgcacactg
      661 tcgccgccat ccgcgcacgc aaggatctca ccgagcggca gaaggacaag atcctgggcg
      721 aaaatgcggc tcagctgctt cgtctttagc gttctcctcg cgccgccggc gttcggcgtg
      781 gagcccacgc ccggcgtggc agccgtcgcc gcgtattcgg gtcccgaccg gacggcgcgc
      841 ctgatcgcgg gcgcgaagaa agagggcgag ctgatgctct actcctccct cacgcaggac
      901 gaccagctca agctcgtcgc cgacttcaag cgccgctacg gggtcacggt gaaattctgg
      961 cgcggcagcc aggcgcatat cctgcagcgc gtcacgagcg aaacgcgcgg gggtcgcttc
     1021 gagttcgacg tgctcgagac caacgccccg cagatcgagg cgctcgcgcg ggaaaagctg
     1081 ctgcagaaaa tgaactcgcc ctacgtcgag caggaattgc tgcccgaggt catgccgagc
     1141 cacggcgagt gggcgcccga ccggctcaac ctgctcgtct acgcctacaa cacccacttg
     1201 gtgaagcctg cggacgtacc gaagtcctgg caggatctcc tcgacccgaa atggaaaaag
     1261 cgcatcggcg tggaatccac gaacgtggaa tggttcgccg ctctggtcaa atcactcggc
     1321 gagaaagagg ggctcgagct gttcaggcgc atggccgaca acggcctggc ggtgcgcacc
     1381 ggccacaacc acagtgcggt cctggtcgca gccggagaaa ttcccctgat gctcgggatc
     1441 tacagccacg acgccgagcg catgaaggcc aagtccgcgc cggtcgactg gttcgtcctg
     1501 ccgccggcgg tcgtgctggc ctccgcagtc gcggtctcgc ggcgcgcacc gcgtcccaac
     1561 gccgcggccc ttttctacga ctacatgctg accgacggcc agcgctttta cacggaagtg
     1621 caccgcgtgc ctgcgaacaa gaactacgac acgccggtgc ggcggctggt gcgcgagcgc
     1681 caagccgtca aaatcgtgaa tgctcaggaa accgccgacg aatacgaaaa gtggctggac
     1741 ctctacaagc ggatcgtcgt cgaccgctcg cagcactgat ccctctcggc gcatgaagag
     1801 caggtcggtt cgcctccgtc tttacgcggc ggcgttcgcc gcgctcgcgg cgctgggctg
     1861 cgcggcccag accgtaatcc acgaaaggac ctcgccttac aacacggtca tcgtcaccga
     1921 ggactacagc ggcatgcgca cgctgctctt cgaaagaggc gggggtcggc aaagcgtcgt
     1981 gaaaccggga gatccggatc atctggagct tccctacgcg cgggtcgccc tcgccggact
     2041 cgcgctgtgc gaggaaccgc gccgcatcct cgtcgtcggt ctcggcggag ggagtttgcc
     2101 catgttcctg cgcgcgcact atcccggggc gatgatcgac gtggcggaga tcgaccccga
     2161 ggtcgtggac gtcgcgaaga agttcttcgg cttcgtcgag gacgagcgca tgcgggcccg
     2221 cgtcggcgac gggcggcaat t
//
LOCUS       DASPZD010000067         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1320751, whole genome shotgun sequence.
ACCESSION   DASPZD010000067 DASPZD010000000
VERSION     DASPZD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320751"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VEM33_01250"
     CDS             <1..481
                     /locus_tag="VEM33_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYS50484.1"
                     /translation="TVQIPGPDGGPFKTRVLPPLQSPAAPQKSESTRPAKDPTSKAMP
                     AEPEAASRPRAAKKESAKSAERAKTQVAEARRAQALLNDEGFVVPLGAFTNPDNAKQV
                     QDRAASAGIKSYTESVTGQQGEQTRVRAGPFASRDAAEKARDRLKSLGLEVGQVAQR"
     gene            478..966
                     /locus_tag="VEM33_01255"
     CDS             478..966
                     /locus_tag="VEM33_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HYS50485.1"
                     /translation="MTWFDFGVLVALVVSIGISLLHGLAREMVSLGVWVGGFILATLF
                     GGHVAGFLPQSLGPLLSALVGFLIVFGVVLIVGWLVGLALSSAVRASGLAPADRALGS
                     VFGLVRGLIIVLVVVLLAGLTPLPREPFWRDAALSGPFETAVLALRPYMPARLAQRLK
                     YR"
     gene            986..2500
                     /gene="purF"
                     /locus_tag="VEM33_01260"
     CDS             986..2500
                     /gene="purF"
                     /locus_tag="VEM33_01260"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYS50486.1"
                     /translation="MCGILGVVAKTPVNQLLYDGLLLLQHRGQDAAGIVTSEHKAFHM
                     HKGPGMVRDVFRTRNMRSLSGGTGIGHCRYPTAGSAFNAGEAQPFYVNSPFGIVLGHN
                     GNLTNSEVLKQEMFRQDLRHINTNSDSEVLLNVLAHELELAAKGQKLDPETIFTSVAN
                     VHRRCRGAYAVVALIAGYGLLAFRDPFGIRPLVFGRNHTDEGPEYLVASESVALTAFG
                     FELVRDILPGEAIFIDESGTFNSRQCAAKPVLAPCIFEYVYLARPDSVMDAVSVYESR
                     LHMGRKLSEKIKRTMPHLEIDVVIPVPDTSRPAALELAHGLNVTYREGFIRNRYIGRT
                     FIMPGQTIRKKSVRQKLSVVGLEFKDKNVLIVDDSIVRGTTGREIVQMAREAGASKVF
                     FASAAPPVRFPNVYGIDMPTRAELIATGRNEEQVAREIGADAVIYQDLDALKDAVRAV
                     NPKLTQFETSCFDGVYITGDVTSEYLASIERQRDAGRQESVEDSEDNQLDLNAA"
     gene            2680..>3084
                     /locus_tag="VEM33_01265"
     CDS             2680..>3084
                     /locus_tag="VEM33_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYS50487.1"
                     /translation="MQDYDIETLAVRAGIARSQFNEHSEALYLTSSFVFENAAEAADR
                     FCNNAPGNIYSRFTNPTVTAFQERLAALEGAQACVATASGMSAILATAMATLKAGDHV
                     VCSSGVFGATVQLFSTVLSKFGIETTYADGTLP"
BASE COUNT          557 a          987 c          995 g          545 t
ORIGIN      
        1 gacggtgcaa atccccggcc ccgacggagg gccgttcaag acccgggttc tcccgccgct
       61 gcagtccccg gcagcgccgc agaagagcga atcgacgcgg cctgcgaagg atccgacgag
      121 caaagcgatg ccagccgagc ccgaggcggc ctcgcgccct cgcgccgcga agaaggagag
      181 cgcgaagagc gcggagcgcg cgaagaccca ggtcgccgag gctcgccgcg cccaggcgct
      241 gttgaacgac gaggggttcg tcgtcccgct cggtgccttt accaaccctg acaacgcgaa
      301 gcaggtgcag gaccgggcgg cttccgcagg catcaagagc tacaccgaga gcgtcacggg
      361 ccagcagggc gagcagacgc gcgtgcgcgc cggacctttc gcgagccgcg acgccgcgga
      421 aaaggcccgc gaccggctca agtccctcgg cctcgaagtc gggcaggtgg cacaacgatg
      481 acgtggttcg actttggcgt actggtcgcc ctggtcgtgt cgatcgggat cagcctgctc
      541 cacgggctcg cgcgcgagat ggtctcgctc ggagtgtggg tcggcggatt catattggcg
      601 acgctgttcg gcggccacgt cgctggcttc ctgccgcaga gcctgggtcc gttgctgagc
      661 gcgctggtag gcttcctgat agtgttcggc gtggttctga tcgtcgggtg gctcgtcggt
      721 cttgcgcttt cgtctgccgt ccgcgcatcc ggtctggctc cggcggatcg cgcgctgggg
      781 tcggtcttcg gcctggtaag agggttgatc atcgtgctcg tcgtggtgtt gcttgcgggc
      841 ctgacgccgc ttccgcgcga acctttctgg cgcgacgcgg cgctttcggg gccgttcgag
      901 accgcggtac tcgcgctgcg gccctatatg cccgcgcgct tggcgcaacg cctgaagtac
      961 cgatgagtcg agaggaatag cgcagatgtg cggcattctc ggagtcgtgg cgaagacgcc
     1021 ggtcaatcag ctgctgtacg acgggctgct gctgctgcag caccggggcc aagacgcagc
     1081 cgggatcgtc acatcggagc acaaggcgtt tcacatgcac aaggggcccg ggatggtgcg
     1141 cgacgtgttt cgcacccgga acatgcgctc gctttccggc ggcaccggca tcgggcactg
     1201 ccgctatccc accgcgggct cggcgttcaa cgcaggcgag gcacaacctt tctatgtcaa
     1261 ctctccattt gggatcgtcc tcggccacaa cggcaacctg accaattccg aggtgctgaa
     1321 gcaggagatg ttccgtcagg atctgcgcca catcaatacc aactccgatt cggaagtgct
     1381 gctcaacgtg ctcgcccacg aactggagct cgccgcaaag ggtcagaagc tcgaccccga
     1441 gaccatcttt acgtcggtgg caaacgtgca ccggcgctgc cgcggcgcct acgccgtggt
     1501 ggccttgatt gcgggctacg ggctgctcgc gttccgcgac ccgttcggaa tccgtccgct
     1561 cgtgttcggc cgcaatcaca ccgacgaagg acccgagtat ctggtcgcgt cagaaagcgt
     1621 cgcgctcacc gcattcggct tcgagctggt gcgggacatc ctgccgggcg aggccatctt
     1681 catcgatgaa agcggaacgt tcaacagccg ccaatgtgcg gctaaacccg tcctcgcgcc
     1741 ctgcatcttc gaatacgtct atcttgcccg gcccgactcg gtcatggacg cggtttcggt
     1801 gtacgagagc cggctgcaca tggggcggaa gctctcggaa aagatcaagc gcacgatgcc
     1861 tcatctcgag atcgacgtcg tgatcccggt tcccgacaca agcaggccgg ctgcgctgga
     1921 gctggctcac ggcctcaacg tgacgtatag agaaggcttt atccgcaatc gctacatcgg
     1981 ccgtactttc atcatgcccg gtcagaccat tcgcaagaag tcggtacgcc agaagctgag
     2041 cgtagtcggc ctcgaattca aggacaagaa cgtcctgatc gtggacgact cgatcgtgcg
     2101 gggaaccacc ggccgcgaaa tcgtgcagat ggcacgcgaa gcgggcgcca gcaaggtgtt
     2161 tttcgcgtct gcagcgccgc cggtgcgatt cccgaacgtg tatggcatcg acatgccgac
     2221 gcgtgccgag ctgatcgcca ccgggcggaa cgaggagcag gtcgcacgcg agattggggc
     2281 ggatgcggtc atctatcagg atctggacgc gctcaaggac gcggtacgtg cggtcaatcc
     2341 gaagctcacg cagttcgaga cctcgtgttt cgacggggta tacatcaccg gagacgtcac
     2401 cagcgagtac cttgcctcga tcgaacggca gcgcgacgcc ggccgccagg aaagcgtgga
     2461 ggactccgag gacaaccagc ttgacctcaa cgcggcctga acgcgcctgc ccgaagctcc
     2521 tcgaccgccg gcaggtttga catcgccgcg ccgggatagt aaggtgcgct ctgcgccgat
     2581 ttgatcgaca gcttgcgggc gcctaataaa tacggctaaa gcgtggccaa aacccgtctt
     2641 agcgaaagct ggacgggttt ttcattttca ggatcgagaa tgcaggacta cgatatcgaa
     2701 acgcttgcgg tgcgcgccgg aatcgcgcgc agccagttca acgagcactc cgaggcgctc
     2761 tatctcacct cgagcttcgt tttcgagaac gcagcggaag ccgcggaccg gttctgcaac
     2821 aacgcgcccg ggaacatcta ttcgcgcttc accaatccca ccgtgaccgc gttccaggag
     2881 cggctcgccg ctctcgaagg cgcgcaggcc tgcgtcgcga ccgcctccgg gatgtcggcc
     2941 attcttgcga ccgccatggc cacgctgaaa gccggagatc acgtcgtgtg ctcctccggg
     3001 gtcttcggcg cgaccgtgca gttgttcagc accgtccttt ccaagttcgg aatcgagaca
     3061 acgtacgcgg acggcaccct gcct
//
LOCUS       DASPZD010000068         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_206304, whole genome shotgun sequence.
ACCESSION   DASPZD010000068 DASPZD010000000
VERSION     DASPZD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206304"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VEM33_01270"
     CDS             <1..327
                     /locus_tag="VEM33_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYS50488.1"
                     /translation="VIIRIGSAFRSVDRQAEIIGAKLAEGVSLDAVLCVSAPPGYSEH
                     HSGRAVDVTTDEGAPLEPEFEKTEAFRWLSKNAVRFGFVLSYPAGNPYGYDYEPWHWC
                     FRAVEV"
     gene            356..958
                     /locus_tag="VEM33_01275"
     CDS             356..958
                     /locus_tag="VEM33_01275"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HYS50489.1"
                     /translation="MLLLPALCGNASCHAQEKANTVPETRPGREMIVEVQELIPVESN
                     RKIRTTNLDLLFRIRTFDTPDFKFHGGLTVSHSRGTITRQTGSFEAGTLKNEKYDSTA
                     TGAGPVLAVQAELGRGRNLSTSLHGSAGVLLYDRDFPAGASRYDFMLRYGLTFSYRLN
                     DRQDLSLTLRGMHVSNGQGINSHNPQYNAMGVGVQYLRAF"
     gene            complement(988..>2683)
                     /gene="glnE"
                     /locus_tag="VEM33_01280"
     CDS             complement(988..>2683)
                     /gene="glnE"
                     /locus_tag="VEM33_01280"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HYS50490.1"
                     /translation="LAQKKLLPDTAAEELSAAYVFLRRLEHRLQYLDDAQTHELPEET
                     EDRSLVARAMGFDSWDAFRAALEEHRARVSSHFEQVFSAEEVSKHALAPLWTGPEQAE
                     THLAQLGFRDAAGTAARLAAVRSSPRYVGLPQASRTRFDALIPRIIEESAAQEDPDAA
                     LVRSLEFMETVSRRAAYLALLQEHPAALARLAQLLGASSWAAEYLNRHPVVLDELLDA
                     RALLARPDWSAFAQELRGQLASRQGDEERQMDWLREAHHAQVFRLLAQDLSGMLSVEK
                     LADDLSDLADAMLQATLELCWAQLRERHREEPRFAIVGYGKLGGKELGYASDLDLIFL
                     YDDPDDRAPEIYARLAQRLSRWLTSRTAAGVLFDIDLRLRPEGGSGLLVSSIDAFRRY
                     QRESAWTWEHQALTRARFCAGEPAVGTEFEEERRGILRIARDEAKLRRDVLSMREQLL
                     QGHPNESGLFDLKHDRDGMIDIEFIVQMLVLAHAHRRPELTANSGNIALLKLAAKLRL
                     IPGDSAEAVADAYREYRRRQHRLRLNGARYARVPAEEVRNHMAATRALWRRVFASE"
BASE COUNT          453 a          893 c          890 g          447 t
ORIGIN      
        1 gtgatcatca ggatcggctc ggccttccga agcgtcgatc gccaggccga gataatcggc
       61 gcgaagctcg ccgaaggcgt gtccctggac gcagtcctct gcgtgagcgc tcctcccggt
      121 tacagcgagc atcactccgg ccgcgcggtt gatgtgacaa ccgacgaggg tgcgcctctc
      181 gagcccgagt tcgaaaagac cgaggcattt cgctggctgt cgaagaacgc ggtccggttc
      241 ggtttcgttc tctcgtatcc ggcggggaat ccgtacggtt acgactatga gccgtggcac
      301 tggtgcttca gggcggttga ggtctaacag gcgattgaag atcacggcgc tttcgatgtt
      361 gctgcttccc gccctgtgcg ggaacgcttc gtgccacgct caggaaaaag cgaataccgt
      421 accggagacg cggcccgggc gggagatgat cgttgaagtg caggagctca taccggtcga
      481 aagcaaccgc aaaatccgca ccacgaacct cgaccttctc ttccgcatca ggacatttga
      541 tactccggac ttcaagttcc atggaggcct tacggtgtcc cactcccgcg gaaccatcac
      601 gcggcagaca ggctcattcg aggcgggcac tctcaagaac gaaaagtacg acagcaccgc
      661 gaccggtgcg ggacccgttc tcgcagttca ggccgaactc gggcgcggca ggaacctctc
      721 cacttcgctg catggaagcg cgggcgtcct tctttacgac agggatttcc ccgcgggcgc
      781 ttcccgttat gacttcatgc tccggtacgg cctcactttt tcttacagat tgaacgacag
      841 gcaggacctt tccctgaccc tcaggggtat gcatgtgtcg aacggccagg gcatcaactc
      901 ccacaacccg caatacaacg cgatgggcgt gggcgtgcaa tacctgaggg ccttttgacg
      961 gcattcatgc gacgcagggg cgagtagtca ctcgcttgcg aacaccctcc gccacagggc
     1021 gcgcgtcgcg gccatgtggt ttctcacctc ctccgcgggc acgcgtgcgt acctcgcgcc
     1081 gttcaggcgc agccggtgct gccggcggcg gtattcgcgg taggcgtcgg ccaccgcttc
     1141 ggcggaatcg ccgggaatca agcgcagctt cgcggcgagc ttcagcaggg cgatgttgcc
     1201 gctgttggcg gtgagctcgg gacggcggtg cgcgtgagcg agcaccagca tctgaacgat
     1261 gaactcgatg tcgatcatgc cgtcacggtc gtgcttgagg tcgaacagcc cgctctcgtt
     1321 cgggtggccc tggaggagct gctcgcgcat cgacagcacg tcgcggcgca gcttcgcttc
     1381 gtcgcgcgcg atccggagga tcccgcgccg ctcctcctcg aactcggttc ccacggccgg
     1441 ctcgcccgcg cagaatcgcg cgcgcgtgag cgcctggtgc tcccaggtcc aggcggattc
     1501 gcgctggtag cggcgaaagg cgtcgatcga gctgacgagg aggccgctcc cgccttcggg
     1561 gcgcaggcgc aggtcgatgt cgaagagaac gcccgcggca gtgcggctgg tgagccagcg
     1621 gctcaggcgc tgcgcgaggc gcgcgtagat ttccggcgcc cggtcgtccg gatcgtcgta
     1681 aagaaagatg agatcgaggt cggacgcgta gcccagctcc ttgccgccga gcttgccgta
     1741 gccgacgatc gcaaaacgcg gctcctcgcg gtggcgctcg cgcagctgcg cccagcacag
     1801 ctccagcgtc gcctgcagca tggcgtcggc gagatcggac agatcgtcgg ccagcttttc
     1861 gacggaaagc atgcccgaca gatcctgcgc gagcaggcgg aagacctgtg cgtgatgcgc
     1921 ctcgcgcagc caatccatct gccgctcctc gtcgccctgc cgggaggcga gctgaccccg
     1981 gagctcctgg gcgaaagcgc tccaatccgg ccgcgcgagc aaggcgcgcg cgtcgagcag
     2041 ctcgtcgagc acgaccggat gccggttgag atattccgcc gcccagctcg acgcgccgag
     2101 caactgcgcg aggcgcgcga gcgcggccgg gtgctcctgg agaagcgcga gatacgcggc
     2161 gcggcggctg accgtctcca tgaactccag gctgcgcacg agggccgcgt ccgggtcctc
     2221 ctgcgccgcc gattcctcga tgatcctcgg aatcagggcg tcgaagcgcg tccggctcgc
     2281 ctgcggcagg ccgacatagc gcgggctcga gcgcaccgcc gcaagccgcg ccgcggttcc
     2341 cgcggcgtcg cgaaacccga gctgcgccag gtgcgtttcg gcctgctccg gcccggtcca
     2401 cagcggcgcg agcgcgtgct tcgagacctc ctcggccgaa aacacctgct cgaaatgcga
     2461 ggacacgcgg gcccggtgct cctcgagcgc ggcgcggaac gcatcccagg aatcgaagcc
     2521 catggcccgg gcgaccaaag agcgatcctc agtctcctcc ggtagctcgt gtgtctgcgc
     2581 gtcgtccagg tactggagcc ggtgctcaag gcgccgcagg aacacgtagg ccgcggaaag
     2641 ctcctcggcc gccgtatcgg ggagcagctt cttttgcgcg agc
//
LOCUS       DASPZD010000069         4163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_326096, whole genome shotgun sequence.
ACCESSION   DASPZD010000069 DASPZD010000000
VERSION     DASPZD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326096"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /gene="pyrC"
                     /locus_tag="VEM33_01285"
     CDS             <1..959
                     /gene="pyrC"
                     /locus_tag="VEM33_01285"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYS50491.1"
                     /translation="LPHTARVFARAVVMPNLRPPITDTALATAYRGRVLKALPKGSDF
                     EPLMTLYLTDGTSAEEIARAKRSGFVCGVKYYPAGATTNSGAGVTSIERAYPALDAMQ
                     EHDLPLLVHGEVTTPGVDVFDRERVFLETVLAGIVRRFPRLRIVVEHVTTGEAVDFVL
                     TGGPGIAATITAHHLLYNRSAMFAGGLRPHHYCLPVLKREEHREALVRAATSGNPKFF
                     LGTDSAPHARADKETPCGHAGIYTAHAAIELYAEVFERAGVLDKLEAFASFHGADFYR
                     LRRNRGTITLRKETWSVPGEMPFGTAMLVPLRAGESVGWRLA"
     gene            944..1783
                     /locus_tag="VEM33_01290"
     CDS             944..1783
                     /locus_tag="VEM33_01290"
                     /inference="COORDINATES: protein motif:HMM:NF022673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HYS50492.1"
                     /translation="MAARLTRMPGRGMPVLDAPIFTALMPLLSRLPPDRFPQHDELSA
                     LAAPAVVSGGGAPIRFVPPAAVSEEFSAQYEVRIFETGEVQTRPGSWHDLFNALVWLA
                     FPKTKAVLNGHHYDEIHARRGERLRGTARDVLTLFDEGGIVVAAADSGLPALLREFRW
                     KELFWRRRAQVLRSMRFYVFGHAIYEKALEPYKGVTAKALILDVAPGLLDAPIERQLS
                     DLDARAADYFSGTQALASTRSLSPLPILGIPGWEPANASEEYYDDPSQFRPRSKTGDR
                     PRF"
     gene            complement(1823..2335)
                     /locus_tag="VEM33_01295"
     CDS             complement(1823..2335)
                     /locus_tag="VEM33_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HYS50493.1"
                     /translation="MRRRMYFLLPDLGSAIQTANDLLLARVEDGHMHFLAKRGMSLGQ
                     LHEANYLQKSDAVHGAELGFVIGGLGGFLIGIYIYLTPPEGVTLKLVTVLIATVLGAV
                     FGAWAASLVAMSVPNTRLKPFQKDIDAGKILLMVDVPTSRVEELRELVTRRHPEAMAH
                     GIEPTIPAFP"
     gene            2434..2819
                     /gene="rnpB"
                     /locus_tag="VEM33_01300"
     ncRNA           2434..2819
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEM33_01300"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2909..3718)
                     /locus_tag="VEM33_01305"
     CDS             complement(2909..3718)
                     /locus_tag="VEM33_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50494.1"
                     /translation="MSSTHLKRLALLASIAMSLALAPAARALQMVTTSLSEPMNLQAT
                     VSTFNCENSPGPWINFEGAVTLGGMNVQMIFRNNINKDVHTLVEDIQTVAGVTAGTTV
                     RIPKQPSHPFEGGTGTGVGGNPWISIQLVDTNGNPLSGEVPLGRCVQGAFAPSIDFSA
                     TVIGQAIIETLDCTNNPGPHINVSGSMSVSPGVKARIIFRNQRTAGAPHEAVAMAEVT
                     LLSNGFAFSFPKQPVLGGVGGNPWISVRFANPEPIGPETLLGRCVQLLPGN"
BASE COUNT          755 a         1361 c         1300 g          747 t
ORIGIN      
        1 tgcttcccca cacggcgcgc gtatttgcgc gcgcggtcgt catgccgaat ctccggcctc
       61 cgattaccga taccgcgctc gccacggcct accgtggccg cgtgctgaag gccctcccga
      121 agggctcaga tttcgagccc ctcatgaccc tctatttgac cgacggcacg agcgccgagg
      181 agatcgcgcg cgcgaagcga tcgggcttcg tatgcggcgt gaagtactac ccggccggcg
      241 caacgacgaa ctcgggggcg ggggtcacga gcatcgagcg tgcctatccc gcgctcgatg
      301 cgatgcagga gcacgacctg cccctgctgg ttcacgggga agtcacgact ccgggcgtgg
      361 atgttttcga tcgcgaacgc gtgttcctcg agacggtcct cgccggcatc gtccgccgtt
      421 tcccgcgcct tcgcatcgtg gtcgagcatg tgaccaccgg cgaggcggtc gatttcgtgt
      481 tgacgggagg ccccggcatt gccgccacca tcaccgcgca tcacctgctc tacaaccgca
      541 gcgcgatgtt cgcgggcggc ctgcggccac atcattactg tctgccggtc ctgaagcgcg
      601 aggagcaccg cgaggcgctg gtgcgcgccg cgacgagcgg caatcccaaa ttctttctcg
      661 gcaccgacag cgcgccgcac gcccgcgccg acaaggagac gccgtgcggg cacgcgggca
      721 tctataccgc gcacgcggcg atcgagctct atgctgaggt cttcgagcgg gccggggtcc
      781 tcgacaagct cgaagccttc gccagctttc acggggccga tttttaccgc ctgcggcgca
      841 accgcggcac gatcaccctg cgcaaggaga cctggtccgt gcccggcgaa atgccctttg
      901 gcacggcgat gctcgtgccg ctgcgggccg gcgaatccgt cggatggcgg ctcgcctgac
      961 gaggatgccc gggcgcggga tgcccgtgct cgatgcaccg atcttcacgg cgctcatgcc
     1021 tctgctcagc cgcctgccgc cggaccggtt tccccaacac gacgagctca gcgcgcttgc
     1081 ggccccggcg gtggtgagcg gcggcggcgc gccgatacgc ttcgttccgc cagcggcggt
     1141 ctcggaggaa ttctccgctc agtacgaagt gcgcatcttc gagaccgggg aagtgcagac
     1201 gcgcccgggc agctggcacg atctgttcaa cgcgctcgtg tggcttgcgt ttccgaaaac
     1261 caaagcggtt ctgaacggcc accattacga cgaaatccat gcgcgccgcg gcgagcggct
     1321 gaggggtacg gcccgggacg tcctgactct gttcgacgaa ggcgggatcg tcgtcgctgc
     1381 tgcggactcc gggttgccgg cgctgttgcg cgaattcagg tggaaggagc ttttctggag
     1441 gcgccgcgcg caggtgctgc gatccatgcg cttctatgtg ttcggccacg cgatctacga
     1501 aaaggcgctc gaaccttaca agggggtgac cgcgaaggcg ctgatcctgg acgttgcacc
     1561 gggtctgctc gatgcgccga ttgagcggca gctctccgac ctggacgcgc gcgcggcgga
     1621 ctatttctcc ggcacgcagg ctcttgcctc gacccgcagc ctatcgccgc tgccgattct
     1681 cgggattccc ggctgggagc cggccaacgc ctccgaggaa tattacgacg acccctcgca
     1741 attccggccg cgaagtaaaa ccggggacag accccggttt tgagccaacg cctgataaga
     1801 aaaccggggt ctgtccccgg ttttacggaa acgccggaat ggtgggctcg atgccgtgcg
     1861 ccatcgcttc cggatggcgg cgggtgacca gctcccgcag ctcttcaact cgcgacgtcg
     1921 gcacatccac catcaacaaa atcttgccgg cgtcgatgtc cttctggaaa ggcttcagcc
     1981 gcgtgttcgg cacggacatc gcgacgaggc ttgcagccca ggcgccgaag accgcaccga
     2041 gaacggtggc gatcaacacc gtgacaagct tcagcgtgac cccctcgggc ggcgtgagat
     2101 agatatagat gccgatcaaa aacccgccca ggccgccgat tacaaatccc agctcggccc
     2161 catgcaccgc gtcggacttc tgcaggtaat tcgcttcgtg caactggccc agagacatcc
     2221 ctcgcttggc gaggaaatgc atgtgcccat cttcgacccg cgcgagcaac agatcgttgg
     2281 ccgtctggat ggcgctgccc agatcgggca acaggaagta cattctgcgt ctcatgcccg
     2341 tctcctctcc gaaacacgac tcgcggatgg ttcttacccc gcggcattcg cgaagtcaag
     2401 ccgtccatgt cgcaatgcgg tagaatcgtc ggcgaagctg gccagacagt cgctggcaaa
     2461 tgtttcacct gccagaggaa agtccgggct ccacagagca gggtgctggg taacgcccag
     2521 gcgccgcaag gcgacgaaga gggccacaga gacgagtctt gttggacttg acgtggcgcg
     2581 cagcgccacg tcaaagtctc gtttccgcgg cagggtacgg atcgcaagac ccgtcccttg
     2641 ttgacgcgga gcgacaacaa gggtgaaacg cggcaacctc cacccggagc aacatcaaat
     2701 aggcggacga cgacgtggct cgccgagtcc gcgggtagat ggctcgagct ttggggtaac
     2761 tcaaagccta gaggaatgac tgtccacgac agaacccggc ttaccggcca gcttcactcc
     2821 ttccttcgcg tcggtcgacc ggcggacgcg ccaaaaaaaa cggggcgggt aaccccgccc
     2881 cgtcgcgttt gaaaccgcgt gcttattctt agttgcccgg caggagctgg acgcagcgcc
     2941 ccaacagcgt ttcggggccg atcggctccg ggttggcgaa ccggaccgaa atccacggat
     3001 tgccgccgac gccgcccaac accggctgct tggggaacga gaacgcaaat ccgttcgaca
     3061 agagcgtcac ttccgccatc gcgaccgcct cgtgcggcgc gccggccgtc ctctggttcc
     3121 tgaagatgat ccgcgccttc actcccggcg acacgctcat cgaaccgctc acgttgatat
     3181 gaggcccggg gttgttggtg cagtcaaggg tctcgatgat ggcctgcccg atgaccgtag
     3241 cgctgaagtc gatgctcggt gcaaaagctc cctgaacgca gcgaccgagg ggcacttcgc
     3301 cgctcagcgg attgccgttg gtatcgacga gctggatcga gatccacgga ttgccgccca
     3361 cgccggtgcc cgtgcccccc tcgaaaggat gcgagggctg cttggggatc ctgaccgtcg
     3421 ttcccgcggt cacgccggca acggtctgga tgtcctctac gagcgtatgg acgtccttgt
     3481 ttatgttgtt tctgaagatc atttgtacgt tcataccacc gagagtcacc gcgccctcga
     3541 agttgatcca cggcccgggg gagttttcgc agttgaaggt ggacacggtt gcctgcaggt
     3601 tcatcggctc cgagaggctt gtggtaacca tctgcagcgc gcgtgccgcg ggggcgagcg
     3661 ccagactcat tgctatgctc gccagcagcg caagcctctt caggtgggta gaactcatac
     3721 taaatctcct caagtcaagt ctcatcgtta tctccgttgt tggtgattga caaaggttca
     3781 ctgcctgcga cgcgcccctc tgccggccgg cccaaccctc taccggcaaa agggcgcgct
     3841 cagggatggc ggcgttacgc gacgggaaat acgcagcgtt ctcaactccc tgtccaatgc
     3901 gcgaatcaag cttcgcggga cgccgggcca tcacccacag acacgaaaat ggggcccggg
     3961 aggttccttg acttgcatgg tccggtcggc gcgttctctt taaacggagt tgcaacataa
     4021 ttctgatacg ccgccagaaa gaagggcagg tgatgcccgc cccgtattac cagatgacga
     4081 tgaaccttgt gccgtcttca gcgcgccgcg ctccgaaccc actccgtgac cgccgcgagg
     4141 atctcgggtg aatcgccgag cgc
//
LOCUS       DASPZD010000070         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1295596, whole genome shotgun sequence.
ACCESSION   DASPZD010000070 DASPZD010000000
VERSION     DASPZD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295596"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VEM33_01310"
     CDS             complement(<1..332)
                     /locus_tag="VEM33_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HYS50495.1"
                     /translation="MNEFKKDFQDMQWRFSDRSGLRARDENVYWTPIVVTKREIDAET
                     ERLASLPSPANGRRESLIVHPLAEANAPGFAPGIQVKLSVLKPGEKSAAFRHNATEVN
                     FCIGGSGHT"
     gene            complement(367..1365)
                     /locus_tag="VEM33_01315"
     CDS             complement(367..1365)
                     /locus_tag="VEM33_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HYS50496.1"
                     /translation="MSADAIWITEAEVVQLMDLREAIVALEAALREEARGEAHNMTKT
                     LLQYGKNNLHAIGGKLGNLVGTKTWTHTQGGTCPLLLLWSADDGSLVAVIEAFALGNL
                     RTGGISGVAADWMAKKDAKVMAIVGTGKQALAQVGTMLAVRPIEKLRIHSPREDSRQA
                     FAAKAREEFGMETVTCASASEACRGAQIVTLVTRAAQPFLTAAMLDKGAHLNAVGAIA
                     PDREEFAQDVFARATLVAVDNLPGVQQLSREFMTRYASSGWEKVMPLSGLIASGRGRS
                     GTDDVSLFKAMGMGISDLALGVELLRRARELGAGRAIPQPKKTKPRLAGARAKNPV"
     gene            complement(1368..2348)
                     /locus_tag="VEM33_01320"
     CDS             complement(1368..2348)
                     /locus_tag="VEM33_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HYS50497.1"
                     /translation="MERLSLTLAIGDYEHTRDLATGEVPVQGVSLNVLNLSPEEAFFR
                     FTFFREWEVSEMSMGKYVSLRSQEDNSIAAIPVFPSRVFRQSMIYVREGGKIDRPEQL
                     KGKRIGVPEWAQTAVIYSRGYLSRQAKVPLASVEWIQAGVNEAGRVEKVQPKLPEGVR
                     LRPEPQHSLNDMLLAGDIDAVLSARPPRSFGSGIRRLFPDYEAVEAAYFRETGIFPIM
                     HVIVIKTDVLERHPWLAMNLYKAFEEAKRRSVGRLSDITASHAPLAWLAPYTQRMKDL
                     FGEDFWPYGLEKNRTTLQAFVDFAFEQGVCHRRLELEELFPKQVLTSFKV"
     gene            complement(2377..2667)
                     /locus_tag="VEM33_01325"
                     /pseudo
     CDS             complement(2377..2667)
                     /locus_tag="VEM33_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
     gene            complement(2669..>3041)
                     /locus_tag="VEM33_01330"
     CDS             complement(2669..>3041)
                     /locus_tag="VEM33_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="salicylate hydroxylase"
                     /protein_id="HYS50498.1"
                     /translation="EQCLYKIVPRENFERGLPLATWLCESKRYLADRVTAIRKTAVYA
                     PRYVRRMVSGIRVLGWKEGMLEVRANYVALETLQDELTRVFNAGQYRDKLVVEESRLK
                     FKEKICIFDSLLVPNSLIYPL"
BASE COUNT          519 a         1008 c          916 g          598 t
ORIGIN      
        1 gtatggcccg aaccgccgat gcagaaattc acctcggtgg cgttgtgccg gaacgcggcg
       61 ctcttctcgc ccggcttcag caccgagagt ttcacctgga tgcccggcgc gaagcccggc
      121 gcattggcct cggctagcgg atgcacgatc agcgactcgc gccgcccgtt cgcgggcgag
      181 ggcaggctcg cgagccgctc ggtttcggca tcgatctcgc gcttggtgac cacgatcggc
      241 gtccaataca cgttctcgtc ccgcgcgcgc agaccggagc ggtcggagaa gcgccactgc
      301 atgtcctgaa aatccttctt gaactcgttc ataagacctc ctcggccgag gaacagatcg
      361 tactccctag acggggtttt tggctctcgc acccgccagc cgcggcttcg tcttcttcgg
      421 ctgcgggatt gcacggcccg cgccgagctc tctcgcccgc ctgagcaatt ccacgccgag
      481 cgcgagatcg gaaatgccca tgcccatcgc cttgaaaagc gatacgtcgt ccgtgcccga
      541 gcgtccgcgg cccgatgcga taagcccgga gagcggcatc actttctccc atccggacga
      601 tgcatatcga gtcatgaatt cgcgcgagag ctgctgcacg cccggaagat tgtccaccgc
      661 caccagcgtg gcgcgcgcga agacgtcctg cgcgaattcc tcccggtccg gcgcgatcgc
      721 gcccacggcg ttcaggtgag cgcctttgtc gagcattgca gcggtgagaa acggctgcgc
      781 cgccctcgtc accagcgtca cgatttgcgc gcccctgcat gcctcgctcg cggaggcgca
      841 ggtcacagtt tccatgccga actcctcgcg agcttttgcg gcgaacgcct ggcgcgagtc
      901 ttcgcgcggg ctgtggatac gcagcttttc gatcggccgc accgcgagca tggtgccgac
      961 ctgtgcgagc gcctgctttc ccgtgccgac gatcgccatc accttggcat cctttttcgc
     1021 catccagtcg gccgccacgc ccgaaatgcc gccggtgcgc aggttgccga gggcgaaagc
     1081 ctcgatcacc gcgaccagag acccgtcatc ggcgctccag agaagcaaaa gcgggcaagt
     1141 cccaccttgc gtgtgcgtcc aggtcttggt gcccaccaga ttcccgagct tgccgccgat
     1201 ggcgtgcagg ttgttctttc catactgcag caaggtcttc gtcatgttgt gcgcttcgcc
     1261 gcgggcttcc tcgcgcagcg cggcctcgag agcgacgatc gcctccctca agtccatcag
     1321 ctggacgact tcggcttcgg tgatccagat cgcgtccgcg ctcattttca gaccttgaac
     1381 gaggtcagca cctgcttggg aaacagctcc tcgagctcaa ggcgccgatg gcacacaccc
     1441 tgttcgaatg caaaatcgac gaaggcctgg agcgtggtgc ggttcttctc cagcccgtaa
     1501 ggccagaagt cctcgccgaa gaggtctttc atgcgctggg tgtacggcgc aagccaggcg
     1561 agcggcgcgt gcgaagccgt gatgtcggaa agccgcccga ccgagcggcg cttcgcttcc
     1621 tcgaaggcct tgtagagatt catcgcgagc caggggtggc gctcgagcac gtcggttttt
     1681 atcacgatca catgcatgat aggaaagatt ccggtctccc tgaaataggc cgcttcgacc
     1741 gcttcgtaat ccggaaacag ccgccggatc ccgctgccga agctgcgcgg aggccgtgcc
     1801 gaaagcacgg cgtcgatatc ccccgcgagc agcatgtcgt tcagggagtg ttgcggctcc
     1861 gggcggagcc gcactccttc ggggagcttc ggctgcactt tctccacgcg ccccgcttcg
     1921 ttcacccccg cctggatcca ctccaccgac gcgagcggca ccttggcctg gcggctgagg
     1981 tagccgcgcg agtagatcac cgccgtctgc gcccactccg gcaccccgat acgcttgccc
     2041 ttcagctgct ccgggcgatc gatcttgccg ccctcgcgca cgtagatcat cgattgccgg
     2101 aacacccgcg acggaaatac gggaatcgcg gcgatgctgt tgtcctcctg cgagcgcaag
     2161 gacacatact tccccatcga catttccgac acttcccatt cccggaagaa ggtgaagcga
     2221 aagaaggctt cctccggcga caggttgagc acgttgaggc tcacgccctg gacgggcact
     2281 tctcccgtgg cgaggtcgcg cgtgtgctcg tagtcgccga tcgctagcgt gagggacaat
     2341 ctttccacgg ccgcgcgatt ccctgagcag caccgcgccg ttttcgatct ttacggcgta
     2401 agtgcgaatg tcctcggtgc acggagcgcc gaccgccttg ccgctcgcga tgtgaaactt
     2461 cccctggtgc agggggcatt cgatctcttc gccctcgatg aaaccgtcgg cgaggcttgc
     2521 ctgcccgtgg gtgcaggtgt cgtgcgtggc gaaaatcttg ccgccaagat tgtaaaggca
     2581 caccggctct gctctgagat tgacgagcag cgttccgtcc tcgggtacat cggacgcgtc
     2641 ggcgactttt atccagctct cgctcatgtc aaaggggata gatcaggctg ttcggaacga
     2701 ggaggctgtc gaaaatgcag atcttttcct tgaattttaa cctgctctcc tcgacgacga
     2761 gcttgtcgcg atattgaccg gcgttgaaga ctcgcgtgag ctcgtcttgc agcgtctcga
     2821 gcgcgacata gttcgcgcgc acctcgagca ttccttcctt ccagccgagg acgcgaatgc
     2881 cgctcaccat tcggcgcacg taacgcggtg cgtacacggc ggtcttgcgg atcgcggtga
     2941 cccggtcggc gagatacctt ttgctttcgc aaagccaggt cgcgagcggc agcccgcgct
     3001 cgaagttctc gcgcggaacg atcttgtaga gacattgttc g
//
LOCUS       DASPZD010000071         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_808553, whole genome shotgun sequence.
ACCESSION   DASPZD010000071 DASPZD010000000
VERSION     DASPZD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808553"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VEM33_01335"
     CDS             complement(<1..230)
                     /locus_tag="VEM33_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50499.1"
                     /translation="MAFIIECPEKIRAVLNSLEISTDLIAARSLVLHPEAQELVVAAI
                     GDDGREYLLTPAAATKWREMSAAALTDGVIIR"
     gene            complement(235..1242)
                     /gene="holA"
                     /locus_tag="VEM33_01340"
     CDS             complement(235..1242)
                     /gene="holA"
                     /locus_tag="VEM33_01340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HYS50500.1"
                     /translation="MATLRLEQLDSHLSHELKPLYAIHGDEPLLALEAADAIRARARA
                     SGFSERVVLAPERGFDWGELAASGANRSLFGDKKLIELRLAAGKPGADGAGAIEAFCG
                     RLPPEALTLVTLPRLDKAGQASSWFRALEREGIVVNVYPVERARLPAWIAARLARQKQ
                     RANPDTLQFLADCVEGNLLAAHQEIQKLALLLPPGELDFDTVRQAVMSVARYDALKLA
                     EAMLSGERPRLARMLEGLRGEGEAPPRVLWIIAEEIRAICRVQNGIAAGRSLAEVLRE
                     ARVWGDARQSLVGRAAKKFPRETLLAALEHAAKVDRVVKGIVKGDAWDELLQLGLSFA
                     G"
     gene            complement(1245..1760)
                     /gene="lptE"
                     /locus_tag="VEM33_01345"
     CDS             complement(1245..1760)
                     /gene="lptE"
                     /locus_tag="VEM33_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HYS50501.1"
                     /translation="MWSFERSSRAVLACMLAALLAACGFHLRGQAELPFESLHIPGNN
                     PLVVELKRNVAAASRTRLVDKPGEAQAVLGFTEELRDKFILSFSSQGHVSEYQLRYRV
                     GFRVTDAKGAQVYLPTSEILITRDMAYSDAQVLAKEAEEALLFRDMQRDMVQQIMRRL
                     VAAKPATVSIE"
     gene            complement(1747..4398)
                     /gene="leuS"
                     /locus_tag="VEM33_01350"
     CDS             complement(1747..4398)
                     /gene="leuS"
                     /locus_tag="VEM33_01350"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYS50502.1"
                     /translation="MQPQYHPEIVEREAQEHWERTRAFRATEEPPGKGGRPKFYCLSM
                     FPYPSGKLHMGHVRNYTIGDVMTRFHRMRGFNVLQPMGWDAFGLPAENAAMANGVPPA
                     KWTYDNIAYMKKQLQSLGFAIDWERELATCSPRYYRWNQWLFLRMLEKGLVYKKTGTV
                     NWDPVDQTVLANEQVIDGRGWRTGALVEKREIPMYYMRITAYAEELLASLDELPGWPE
                     RVKTMQANWIGKSHGVNIGFPYELAGERKVLRVFTTRADTLMGATFCAVAAEHPLAAH
                     AARSDKKLAAFVEECKRGPVMEADLAQLDKKGMPTGLFVTHPISGEKIEVWVGNYVLM
                     AYGEGAVMGVPGHDERDFAFAKKYGLPIRPVIDVEGKPYSTDAWQPWYADYGRCVNSG
                     KYDGLDYRVAVETIAADLKKKGLGDKQITWRLRDWGISRQRYWGTPIPIVHCDGCGDV
                     PVPDKDLPVVLPENLVPDGTGNPLAKTPSFVNCKCPKCGKPAKRETDTMDTFVDSSWY
                     FLRFACPDQDKAPVDERAQYWMPVDQYIGGIEHAILHLLYSRFWTKVMRDQGLVAPSE
                     PFTRLLTQGMVLNEIFYRKEASGRITYYNPADVEITHDDRGQRIGAVLRSDGKPVESD
                     GIGTMSKSKNNGVDPQDLIEKYGADTARIFVMFASPPEQTLEWSDSGVEGASRFLRRL
                     WLFAAGRGKAAADHASIRPFIPVKDWSSQTLAIREFRRGVHLALKQASYDYDRMQYNT
                     VVSACMKMLNLLEDFSGGPRDASVNAASQAAAEAMGILLRVLYPVAPHIGFALWKDLG
                     YAAQHGDLLTAPWPVVDETALEQDEIELVLQVNGKLRGHMRAPKSAGREQLEQLALAH
                     DAVTRFTNGQAVKKVVVVPGRLVNVVV"
     gene            4509..>4819
                     /locus_tag="VEM33_01355"
     CDS             4509..>4819
                     /locus_tag="VEM33_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYS50503.1"
                     /translation="MLNLLENLNPEQLAAVTLPDQSALILAGAGSGKTRVLTTRIAWL
                     IQTGNVSPSGVLAVTFTNKAAREMLTRISAMLPISTRGMWVGTFHGLCNRLLRAHYRD
                     AG"
BASE COUNT          796 a         1595 c         1545 g          883 t
ORIGIN      
        1 atcctgatga tcacgccgtc ggtgagcgcc gctgcgctca tctcccgcca cttcgtcgcc
       61 gccgcaggcg taagcaggta ttctcggccg tcgtccccga tggctgcgac gaccaactct
      121 tgcgcctcgg gatgcaatac caaagatcgc gccgcgatca ggtcggttga tatctctagg
      181 gaattcaaga ccgccctgat tttctcagga cattcgatga tgaacgccat tcgatcagcc
      241 cgcgaagctc aacccgagct gcagcagctc atcccaggcg tcgcccttga cgatcccctt
      301 gacgacgcga tcgactttgg cggcgtgctc gagcgcggcg agcagtgtct cgcgcgggaa
      361 cttcttcgcg gcgcgcccca cgagcgactg tctcgcgtcg ccccacacgc gcgcttcgcg
      421 cagaacctcc gccagcgacc ggcccgcggc gatgccgttt tgcacgcggc agatggcgcg
      481 gatttcctcc gcgatgatcc acagcacgcg cggaggcgcc tcgccttcgc cgcgcaggcc
      541 ttcgagcatg cgcgcgaggc gcgggcgctc gccagagagc atcgcttcgg cgagcttgag
      601 cgcgtcgtag cgcgctacgc tcatcaccgc ttggcgcacg gtgtcgaagt cgagttcccc
      661 ggggggcagg agaagcgcga gtttctggat ctcctggtgc gcggccagga gattgccttc
      721 gacgcagtcg gcgagaaact gcagggtatc cgggtttgcg cgctgcttct gccgcgcgag
      781 ccgagcggcg atccatgcgg gaagccgcgc gcgctcgacc ggatagacgt tgacgacgat
      841 tccctcgcgc tcgagcgccc tgaaccacga ggacgcctgg ccggccttgt ccaggcgcgg
      901 cagcgtcacg agcgtgagcg cctcgggcgg gagccttccg caaaacgctt cgatcgcccc
      961 ggcgccgtcg gctccgggct tgcccgcggc gaggcgcagc tcgatcagct tcttgtcgcc
     1021 gaagagcgag cggttcgcgc cgctcgcggc gagctcgccc cagtcgaagc cgcgctccgg
     1081 cgcgaggacc acgcgctccg aaaagccgct tgctcgcgcg cgtgcgcgga tcgcgtcggc
     1141 ggcctcgagc gcaagcagcg gctcgtcgcc gtggatcgcg tacagcggct tcagctcgtg
     1201 cgaaagatgg gaatccagct gctcgagcct gagagtggcc atgcctattc gatggagacc
     1261 gtcgccggtt tcgctgcgac caggcgccgc atgatctgct gcaccatgtc cctttgcatg
     1321 tcccggaaga gcagcgcttc ctcggcctcc ttcgcaagca cctgcgcgtc gctgtaggcc
     1381 atgtcgcggg tgatgaggat ttcgctcgtc ggcagataaa cctgcgcccc cttggcgtcg
     1441 gtgacgcgga atccgacgcg gtagcgcaac tgatactcgc tcacgtggcc ctgggagctg
     1501 aaggaaagaa tgaacttgtc gcgcagctcc tcggtgaaac cgagcacggc ctgcgcctcg
     1561 cccggcttgt ccacgaggcg cgtcctgctc gctgcggcga cgttgcgctt cagctcgacc
     1621 acgagcgggt tgttccccgg aatgtgcagc gactcgaacg gcagctccgc ctggccgcgc
     1681 agatggaagc cgcaggccgc gaggagcgcg gcgagcatgc aggcgaggac agcgcgcgag
     1741 gaacgctcaa acgaccacat tgacgagccg tccggggacc acgacgacct tcttcaccgc
     1801 ctggccgttc gtgaagcgcg tcaccgcgtc gtgagcgaga gcgagctgct cgagttgctc
     1861 gcgcccggcg gatttcggcg cgcgcatgtg gccgcggagc ttgccgttga cctggagcac
     1921 gagttcgatt tcgtcttgct ccagtgccgt ttcgtcgacg acgggccagg gtgcggtcag
     1981 gagatcgcca tgctgagcgg cgtaacccag gtccttccac agcgcaaagc ctatgtgcgg
     2041 cgcgacagga tagagcacgc gcaacaggat acccatcgct tccgccgcgg cttgcgaggc
     2101 ggcattcacg cttgcgtctc ttggtcctcc ggaaaaatct tcgagcagat tgagcatctt
     2161 catgcaggcg gagacgacgg tgttgtattg cattcgatca tagtcatacg acgcttgctt
     2221 caatgcaaga tgaactcctc tgcgaaattc gcgtatcgcg agcgtctgtg aggaccaatc
     2281 cttgaccggg atgaatgggc gaatcgaggc gtgatctgcc gcggcctttc cgcgccccgc
     2341 agcgaacagc caaagcctcc ggagaaaccg gctggcacct tcaacgccag aatccgacca
     2401 ttccagcgtc tgctcgggcg gtgacgcgaa catgacgaag atgcgcgccg tgtccgcccc
     2461 gtacttctcg atcaaatcct gcgggtcgac gccgttgttc ttcgacttcg acatggtgcc
     2521 gatgccgtcg gactcgacgg gtttgccgtc ggagcgcaat accgcgccga tgcgctggcc
     2581 cctgtcgtcg tgcgtgatct cgacgtcggc ggggttgtag taggtgatgc ggccgctcgc
     2641 ttccttgcgg tagaagattt cgttcagcac catgccctgg gtcaggagcc gcgtgaacgg
     2701 ctcgctgggc gcgaccaggc cctggtcgcg catcaccttg gtccagaagc gcgagtacag
     2761 gaggtgcagg atcgcgtgct cgatgccgcc gatgtactgg tcgaccggca tccagtactg
     2821 cgcgcgctcg tccaccggtg ccttgtcctg atcggggcag gcaaagcgca ggaagtacca
     2881 ggacgaatcg acgaaagtgt ccatggtgtc ggtttcgcgc ttcgccggct tgccgcattt
     2941 cgggcacttg caattcacga acgacggcgt tttggcaagc ggattgccgg tgccgtccgg
     3001 aacaaggttc tccggcagca ccacgggaag atccttgtcc ggcaccggaa cgtcgccgca
     3061 gccgtcgcag tggacgattg ggatcggcgt gccccaatag cgctggcgcg agatgcccca
     3121 gtcgcgaagg cgccaggtga tctgcttgtc gcccagaccc ttcttcttca ggtccgccgc
     3181 gatcgtttcg acggcgaccc gatagtcgag cccatcgtac ttgccggaat tcacgcagcg
     3241 cccgtaatcg gcgtaccacg gctgccaggc atcggtcgaa tacggtttgc cctcgacatc
     3301 gatcacgggc cttatcggca ggccgtattt tttcgcgaac gcaaaatcgc gctcgtcgtg
     3361 cccgggcacg cccatcaccg cgccttcgcc gtaggccatc aacacgtagt tgccgaccca
     3421 gacttcgatc ttctcgccgc tgatcggatg cgtgacgaaa aggcccgtgg gcatgccttt
     3481 cttgtcgagt tgggcgaggt cggcctccat caccgggccg cgtttgcatt cctcgacgaa
     3541 ggcggcgagc ttcttgtccg agcgggcggc atgcgcggcg agggggtgct cggcagccac
     3601 ggcacagaag gtggcgccca tcagcgtgtc ggcgcgcgtg gtgaacacgc gcaggacctt
     3661 cctctctccc gccagctcat agggaaagcc gatattgacg ccatggctct tgccgatcca
     3721 gttggcctgc atcgtcttga cgcgctcggg ccagccggga agctcgtcga gcgaggcgag
     3781 cagctcctcg gcgtaagcag tgatccgcat gtagtacatc gggatctcgc gcttctcgac
     3841 gagcgccccg gtgcgccagc cgcggccgtc gatcacctgc tcgttcgcaa gcaccgtctg
     3901 gtccaccggg tcccagttca ccgtgccggt cttcttgtag accagaccct tttcgagcat
     3961 gcgcaggaac agccactggt tccagcgata gtatcggggc gagcaggtcg cgagctcgcg
     4021 ctcccagtcg atcgcgaagc ccagcgactg gagctgcttc ttcatgtagg cgatgttgtc
     4081 gtaggtccac ttcgccggcg gcacgccgtt cgccatcgcg gcgttttcgg cgggaagccc
     4141 gaaagcgtcc cagcccatcg gctggagcac gttgaagccc cgcatccggt ggaagcgggt
     4201 catcacgtcg ccgatggtgt aattgcgcac gtgccccata tgcagtttgc ccgatgggta
     4261 ggggaacatc gacaggcagt agaacttcgg acggccgcct tttcccggcg gctcctctgt
     4321 cgcgcgaaac gcgcgggtcc gctcccaatg ctcctgcgct tcgcgctcga cgatctcggg
     4381 atgatactgg ggctgcatga gcgggttctg gaaaagcatg aattatagcc gcgcgggtcc
     4441 gcggcgcgcc gctgcgctga aggtcccgta gtggcgtgcg gtaaaatcgc tgattctcga
     4501 gcgcccgcat gctcaatctc ctcgaaaacc tcaatcccga gcagctcgcc gcagtcacgc
     4561 ttcccgacca atcggcgctg attctcgccg gcgccggctc ggggaagacg cgcgttctca
     4621 ccacgcggat cgcctggctg atccagaccg ggaacgtgag cccgagcggc gtccttgccg
     4681 ttaccttcac caacaaggcg gcgcgcgaga tgctcacgcg catctcggcg atgctcccga
     4741 tcagcacccg cggcatgtgg gtcggcacct ttcacggcct gtgcaaccgc ttgctgcgcg
     4801 cgcactaccg cgacgccgg
//
LOCUS       DASPZD010000072         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_85492, whole genome shotgun sequence.
ACCESSION   DASPZD010000072 DASPZD010000000
VERSION     DASPZD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85492"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VEM33_01360"
     CDS             complement(<1..285)
                     /locus_tag="VEM33_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYS50504.1"
                     /translation="MKSYRIAVIAGDGIGKEVVPEGVRVLEAAGRKFGIALKWDEFPW
                     SCEYRLKHGRMMPEDGLATIRNHDAVFFGACGFPGVPDHVSLWELLIPMRR"
     gene            complement(299..1234)
                     /locus_tag="VEM33_01365"
     CDS             complement(299..1234)
                     /locus_tag="VEM33_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HYS50505.1"
                     /translation="MAAAQGKVIITCAVTGSIHTPSMSPHLPVTPEEIAEGAIGAAQA
                     GAAIVHLHAREPADGRPTQDPKMFMRFLPRIKAASNVVINLTTGGAPTMTVEERLQPA
                     LQLKPEVASLNMGSMNFGLYEMLARYKEWKYDWEKPYLAGSDDRIFKNSFKDIAYILQ
                     SCSGNDTRFEIECYDIGHLYTASHFLDRKLVKPPLFIQSVFGIRGGIGPHPEDVMHMK
                     RTADRLFGDQYLWSVLGAGRNQMFVAAMSAVMGGNVRVGLEDSLWLGRGTLAKTNAEQ
                     VTKIRRILEELGLQIATPDEAREMLKLKGGRNVAF"
     gene            complement(1273..2244)
                     /locus_tag="VEM33_01370"
     CDS             complement(1273..2244)
                     /locus_tag="VEM33_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50506.1"
                     /translation="MRHWIAPIALALLAGPAFAQDAYPSRPITMVIAFPPGGVTDVTA
                     RPTALAMERALKQRVIVENKPGAAGATGNAYVANSRPDGYTVLMALSSISVIPEAERL
                     QGNKPPYELAQFAPIALISADPVVLVVRDEAPWKNVREFVADARNRPAKITYSSSGIY
                     GALHMPFTLLEHATGTTLWHVPYNGGGPAMQALLGSQVDVTVGGPAIMIGQIRAKRLR
                     PLASFGDKRLASLPDVPTLKELGIDAEYYIWAGLMTPAATPPAIQQALRNAVRQAVQD
                     PDFRGAMAKIETPVTYLDAPEFREFWDTDAKRLAEAVKRTRPVEQKK"
     gene            2356..>2669
                     /gene="tsaB"
                     /locus_tag="VEM33_01375"
     CDS             2356..>2669
                     /gene="tsaB"
                     /locus_tag="VEM33_01375"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HYS50507.1"
                     /translation="MNLLAIDTSTDFCSVAASRGETLFSRHERAGRRQAETILGMVDE
                     VLAEARIELAQIHGIAYGEGPGSFTGLRIAAGVTQGLALARGVGVIGVGSLLALSEEA
                     AK"
BASE COUNT          416 a          891 c          875 g          487 t
ORIGIN      
        1 gcggcgcatc gggatgagca gctcccacag ggacacgtga tcgggcacgc cgggaaaacc
       61 gcaggcgccg aagaacaccg cgtcgtgatt ccggatcgtc gcgaggccgt cttccggcat
      121 catgcgcccg tgcttcagcc ggtattcgca actccaggga aattcgtccc acttgagcgc
      181 gatgccgaat ttcctcccgg cggcctcgag cacgcgcacg ccctccggca cgacttcctt
      241 tccgattccg tcgccggcga taaccgcgat cctgtagctc ttcatcgatc cttcgcctct
      301 agaatgcgac gtttcgtccg cctttgagct tgagcatctc gcgcgcctcg tcgggggtcg
      361 ctatctgcaa tccgagctcc tccagaatcc ggcggatctt cgtcacctgc tccgcattgg
      421 tttttgcgag cgtgccgcgg ccgagccaca ggctgtcctc aagccccacg cgcacgtttc
      481 cccccatgac cgcggacatc gcggcgacga acatctggtt gcggcctgcg ccgagcaccg
      541 accacagata ctggtcgccg aacaggcggt cggcggtgcg cttcatgtgc atcacgtcct
      601 ccggatgcgg tcctatgccg ccgcggatgc cgaacaccga ctggatgaag agcggcggct
      661 tgacgagctt gcggtcgaga aaatgcgacg ctgtatacaa atgcccgatg tcgtagcact
      721 cgatctcgaa gcgggtgtcg ttgccgctgc aggactgcag gatataagcg atgtccttga
      781 agctgttctt gaagatgcga tcgtcgctgc ctgcgaggta gggtttctcc cagtcgtact
      841 tccattcctt gtagcgcgcc agcatctcgt acagcccgaa gttcatcgag cccatgttga
      901 gcgaggcgac ttcgggtttc agttgcagcg ccggctgcag ccgctcctcg accgtcatgg
      961 tcggagcgcc gccggtcgtg agattgatga ccacgttcga cgccgccttg atcctcggca
     1021 ggaagcgcat gaacatcttc ggatcctgcg tcggccgtcc gtccgcgggc tcgcgcgcgt
     1081 gcaggtgcac gatcgcagcg cccgcctgcg ccgcgccgat cgcgccttcg gcgatctcct
     1141 cgggcgtcac cggaaggtgc ggcgacatgc tcggggtgtg gatcgagccg gtgactgcgc
     1201 aggtgatgat gaccttgcct tgagcggcgg ccatacgtca ttccggacga gcgcgggcgt
     1261 tcgctcgcgc cgtcactttt tctgctcgac gggacgggtg cgcttcaccg cctcggctag
     1321 cctcttcgcg tccgtgtccc agaactcccg gaactccggc gcgtcgagat aggtgaccgg
     1381 ggtctcgatc ttggccatgg ccccgcggaa atccggatcc tggaccgcct gccgtaccgc
     1441 gttccgaagc gcctgctgga tcgccggcgg cgtcgccgcc ggggtcatca atccggccca
     1501 gatgtagtac tccgcgtcga taccgagctc cttgagcgtc ggcacgtcgg gaagcgaggc
     1561 gagccgcttg tccccgaagc tcgcgagcgg gcgcagccgc ttggccctga tctggccgat
     1621 catgatcgcc gggccaccga cggtgacatc cacctgcgag ccgagcaggg cttgcatggc
     1681 cggcccgccg ccgttgtagg gcacgtgcca cagcgtcgtc cccgtggcat gctcgagcag
     1741 ggtgaaaggc atgtgcagcg cgccgtagat tccggaggag ctgtaggtga tcttggcggg
     1801 ccgattgcgc gcgtcggcga cgaattcccg gacattcttc cacggcgcct cgtcgcggac
     1861 gaccaggacg accggatcgg ccgagatcag cgcgatcggc gcaaactgcg ccagctcgta
     1921 gggaggcttg ttcccctgga gacgctccgc ctcggggatc accgaaatgg acgagagcgc
     1981 catcagcacg gtgtatccgt cgggcctcga attggcgaca taggcgttcc ccgtcgctcc
     2041 cgccgcgccg ggcttgttct cgacgatgac gcgctgcttc agcgcccttt ccatcgcgag
     2101 ggcggtgggc cgcgcggtga cgtcggtgac gccgcccggc gggaacgcga tcaccatcgt
     2161 gatcgggcgc gacggatagg cgtcctgcgc gaaagcggga cctgcgagaa gcgccagggc
     2221 aatcggcgcg atccagtgac gcatgtgctt tcctccgggc ggatgctcta ttttccggga
     2281 caatcatcgg ggctcgagcg cccggcgtca agcgccgcgg acggctctgc gatccgatag
     2341 aattcccgcg cgaccatgaa cctgctcgcg atcgatacat ccaccgattt ctgctcggtc
     2401 gcggcaagcc gcggcgagac tctgttctcg cgtcacgagc gcgcgggccg gcgccaggcg
     2461 gagacgatcc tcggcatggt cgatgaagtc ctcgccgagg cacgcatcga gctcgcgcag
     2521 atccacggca tcgcctatgg cgaagggccg ggctcgttca ccggccttcg catcgctgca
     2581 ggcgtgacgc agggccttgc gctcgcgcgc ggcgtcggcg tgatcggcgt gggctcgctg
     2641 ctcgccttga gcgaggaagc ggcgaagga
//
LOCUS       DASPZD010000073         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1684713, whole genome shotgun sequence.
ACCESSION   DASPZD010000073 DASPZD010000000
VERSION     DASPZD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684713"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..565
                     /locus_tag="VEM33_01380"
     CDS             23..565
                     /locus_tag="VEM33_01380"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HYS50508.1"
                     /translation="MHTEVHVHGTVLLKSGVSQTDIELALRHWFDYVDVDGLADARSA
                     HQDEPGIVFDRRRRSLDICWTGYVGRNFQHSLEGAFESLSPYSEEAAAVEVSYYQEDG
                     QDEHSWVFVGPTLESIRDMQRRRMIDDLSALLARHFGDAEIGEVTALVNQMFARRAPD
                     APQDRTQVRPGGISGKKHLH"
     gene            855..1298
                     /gene="mraZ"
                     /locus_tag="VEM33_01385"
     CDS             855..1298
                     /gene="mraZ"
                     /locus_tag="VEM33_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYS50509.1"
                     /translation="MLQGATELSLDAKGRLAVPTRYRDALLSGGRAVVLTAHPDSCLL
                     LYPRVAWEPIGAKVQALSSFNEQARWWQRLLVGFAEELELDTQGRILVSPALRKFAGL
                     KKDVMLVGQGSHFEIWDIAAWDERLARGLAQVGTDPPPGTENFSL"
     gene            1295..2236
                     /gene="rsmH"
                     /locus_tag="VEM33_01390"
     CDS             1295..2236
                     /gene="rsmH"
                     /locus_tag="VEM33_01390"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HYS50510.1"
                     /translation="MSVDAHLPVLLRPAVDALAIESRNDGTFVDATFGRGGHSRLILE
                     RLGPRGRLIGMDRDPEAIAAARGIRDPRFTPVHARFSELQARLDVLHAGGVDGVLFDL
                     GVSSPQLDDPQRGFSFRSDGPLDMRMDPTRGQSASEWLERADESEIREVLSGYGEERF
                     AKQIAKAIVAARRRGPVRRTGELAALVAATARTREPGQDPATRTFQALRIHVNQELEE
                     LSLALPQALAALRRSGRLAVISFHSLEDRIVKNFMRGAARPTLPERLPVRARELPPPK
                     LRIVGRPVKPSAEDIKANPRARSAVLRVAEKLVSSPP"
     gene            2233..2508
                     /gene="ftsL"
                     /locus_tag="VEM33_01395"
     CDS             2233..2508
                     /gene="ftsL"
                     /locus_tag="VEM33_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HYS50511.1"
                     /translation="MTRLNLLLVAVLTACALGLVTSQHRARTLFGELEQEQERSHQLG
                     VEYGQLQLEASTWAVHARVERIASRQLHMRVPDAKRLHIVRPQETAK"
     gene            2505..4235
                     /locus_tag="VEM33_01400"
     CDS             2505..4235
                     /locus_tag="VEM33_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYS50512.1"
                     /translation="MNFAASPVLALTLPTWRSRLLLYVFIACFAVLLGRAVYLQGLNN
                     DFLQAKGESRYSRVIEIPANRGRILDRNGEALAISTPVKSIWTIPGEVHPSERQLARL
                     GTLLEMSPREIERRLSDTGRDFVFLKRQMPPEGAERVAGLHIPGLFQNREYRRYYPSG
                     EVTAHVLGFTGVDDIGQEGVELAFQDGLAGKPGSRRVIKDRLGQTVEDVESIRAAQDG
                     HDLTLALDARIQNLAFTQLKAAVAQHRAKAGGVVVVDVDTGELLALANFPTYNPNNRN
                     KLAGAQLRNRALTDTFEPGSTLKPFTVALALEGGRVRPDSVIQIAPGSLSIGASIIHD
                     AHLERSLTVAQVIQKSSNVGAAKIALALSAESMWEMFRDVGFGAAPGLGFPGEAAGKL
                     RPYRSWRPIEQATMSYGHGISVSLVQLARAYSVFARDGELVPLSMLRVEMPPPARRVM
                     APRTAGAVRAMLEMAVNRSGTAPRAQIMGYRVAGKTGTAHKQENGIYATDKYVSSFVG
                     FAPASHPRLVIAVMIDEPSAGRYYGGTVAAPVFAQVMAGALRVLDVPPDGPMQPIELP
                     PEGEEVKESV"
BASE COUNT          775 a         1369 c         1387 g          782 t
ORIGIN      
        1 tcggtgtttc gggggcaggc aaatgcacac tgaagttcat gttcacggca cggtgttgtt
       61 gaagagcggc gtcagccaga ctgacatcga gcttgcgctc agacactggt tcgattacgt
      121 cgacgtggat ggtctcgccg atgcgcgcag cgcccatcag gacgagccgg gcatcgtatt
      181 cgacaggcgc cgccgctctc tcgacatctg ctggaccggg tatgtcggac gcaatttcca
      241 gcattcgctc gagggggcct tcgaatcgct gtcgccctac tccgaggagg cggcggcggt
      301 cgaggtgagc tactaccagg aggacggcca ggatgagcac agctgggtgt tcgtcggtcc
      361 gacgctcgag tcgatccgcg acatgcagcg ccggcgcatg atcgacgacc tttcggccct
      421 gctcgcacgt catttcgggg acgccgagat cggcgaggtg accgccctgg tcaatcagat
      481 gttcgcgcgc cgcgcccccg acgcgccgca ggaccggacc caggtccgcc ccggcggcat
      541 ttccggaaaa aaacacctgc attagaaatc gccaggtgta agtggatccg cctcgtaacc
      601 ggcggccacc agctttttca tcggaagccc ttgatttcct aatgtattac attcacttta
      661 catggtaaca tattaatttg tatattctta tttcatgtca tgcagtttct agaaaagcca
      721 tcttaagtcc ctgttgtagc taaaaatttc atcaatttca ggcttgaccg cctcctctgt
      781 ttcctctaga gtggtaagaa gtagatttta gtggggaact gtggcgtccc attccgccac
      841 gcgaaggggg accgttgctt cagggggcga cagagctgag tctcgacgcc aagggaaggc
      901 ttgctgtgcc tacccggtat cgggacgcct tgttatcggg agggcgcgcg gtggttctca
      961 ccgctcaccc cgatagctgt ctgctccttt atccacgcgt cgcctgggaa ccgatcggcg
     1021 ccaaagttca ggcgctttcc agcttcaacg agcaagcgcg ttggtggcaa cggctgctgg
     1081 tcggcttcgc cgaagagctg gagctcgata ctcaagggcg catcctcgtt tcgcccgcgt
     1141 tgcgcaaatt cgcaggattg aaaaaggacg tgatgctcgt ggggcagggg agccacttcg
     1201 agatctggga cattgcagcg tgggacgaac gtcttgcgcg gggtctcgcg caagtgggca
     1261 ctgacccgcc accgggcaca gaaaacttct cgctgtgagc gtcgatgcgc accttcccgt
     1321 tctcctccgt ccggcagtcg atgcgctcgc gatcgaatca cggaacgacg gcactttcgt
     1381 ggacgcgaca ttcggcagag gcggccacag ccgcctgatt ctggagcggc tgggaccccg
     1441 aggccgcctg atcggaatgg accgggatcc ggaggcgatc gcggctgcgc gcgggatacg
     1501 cgacccgcgc tttacgcctg tccacgcgcg cttcagcgaa ttgcaggcga ggctggacgt
     1561 gctgcacgcg ggcggtgtcg acggagtgct gttcgatctg ggcgtgtcct cgcctcagct
     1621 cgacgacccg cagcgcggct tcagcttccg ttccgacggg ccgctcgaca tgcgcatgga
     1681 ccccacccgg ggtcaaagcg cgtccgagtg gctcgaacgg gcggacgagt ccgagatcag
     1741 ggaggtgctt agcggctatg gcgaagaacg gtttgctaaa cagattgcaa aggcgattgt
     1801 tgctgctagg cgaagagggc ctgtccggcg gaccggggaa cttgccgctc tcgtggctgc
     1861 gaccgcccgg acgcgtgaac cggggcagga tccggcgacg cggacgtttc aggctctacg
     1921 gattcacgtc aatcaggagc ttgaggaatt gtcgctagcg cttccccagg ctctcgccgc
     1981 gctcagacgc tcggggcgcc tcgcggtgat cagcttccat tctctcgagg accgcatcgt
     2041 caagaacttc atgcgcggcg ccgcgcggcc cactctgccc gagcgtctgc ccgtaagggc
     2101 ccgggagctg ccgcctccga agctgcgcat tgtcggccgg cccgtaaagc ccagcgccga
     2161 ggacatcaag gcgaatcccc gcgcgaggag cgccgtgctg cgcgtcgccg agaaacttgt
     2221 ttcaagcccg ccatgacccg cctgaacttg cttctcgtgg ctgtgctgac cgcatgcgcc
     2281 ctgggcttgg tgacctcgca gcaccgggcg cgcacactct ttggcgagct cgagcaggag
     2341 caggaacgtt cgcaccagct cggggtcgaa tacggtcagc tgcagctcga agcaagcacg
     2401 tgggcggtgc acgcacgcgt cgaacgcatc gccagccgcc agctgcacat gcgtgtcccc
     2461 gacgcgaaac gcctccacat cgtgcggccg caggagacgg cgaaatgaat ttcgccgcca
     2521 gtccggttct cgcgctcacc ctgcctacgt ggcgctcgag actcctgctc tacgtcttca
     2581 tcgcatgctt tgccgtgctg ctcggccgcg ccgtctacct gcagggcctg aacaacgatt
     2641 ttctgcaggc aaaaggcgag tcccgctaca gccgggttat cgaaattccg gcaaaccgcg
     2701 gccgcatcct tgaccgcaac ggcgaagcgc tcgccatcag caccccggtt aaatcgattt
     2761 ggacgatccc gggcgaagtg catccaagcg agcggcagct tgcgcgtttg gggacattgc
     2821 tcgagatgtc gccgagggaa atcgaaagac gcctctccga cacgggcagg gactttgttt
     2881 tcctcaagcg gcagatgccg ccggaagggg ccgaacgcgt tgcggggctg cacatccccg
     2941 ggctgttcca gaaccgcgag taccggcgtt attacccaag cggtgaagtc actgcgcacg
     3001 tactcggctt cacgggcgtg gacgacatcg gccaggaggg cgtcgagctc gcgtttcagg
     3061 acgggctcgc cggtaagcct ggcagccgcc gggtgatcaa ggaccgactc ggccagacgg
     3121 tcgaggacgt cgagtcgatc agggcagccc aggacgggca cgacctcact ctcgcactgg
     3181 atgctcgcat ccagaacctc gctttcacgc aactgaaagc ggcggtcgct cagcaccgag
     3241 cgaaggcggg cggcgtggtc gtggtcgacg tcgacacggg cgagctgctc gcgctcgcga
     3301 atttcccgac atacaacccc aacaaccgca acaagcttgc cggcgcgcag ctccggaacc
     3361 gcgcgctgac cgatactttt gaacccggtt cgacgctcaa accctttacc gtcgccctcg
     3421 ctctggaagg ggggcgggtg aggccggatt ccgtgatcca gatcgcccct ggaagcttga
     3481 gcattggggc atccatcatc cacgacgccc atctcgagcg ctcgctcacg gtggcgcagg
     3541 tcatccagaa atcctcaaac gtcggcgcgg cgaagatcgc gctcgcgctc tctgccgagt
     3601 cgatgtggga aatgttccgg gacgtgggct tcggtgccgc gcccggcctc ggtttccccg
     3661 gagaagctgc cggaaagctc cggccgtacc ggagctggcg accgatcgag caggcgacga
     3721 tgtcctacgg gcacggcata tcggtctcgc tcgtgcagct cgcgcgcgcc tacagcgtct
     3781 tcgcgcgcga cggagagctg gttccgctct ccatgcttcg cgtggaaatg ccaccccctg
     3841 cgcggcgcgt gatggccccc agaactgccg gcgcggttcg cgcgatgctc gagatggcgg
     3901 tcaaccggag cggcaccgcg ccccgcgcgc agatcatggg ttatcgcgtc gccggaaaga
     3961 cgggcaccgc gcacaagcag gaaaacggca tctatgccac cgacaagtac gtctcctcgt
     4021 tcgtcggctt cgcgcccgcc tcgcacccgc gcctggtgat cgcagtgatg atcgacgagc
     4081 cctcggccgg ccggtactac ggtgggaccg tggcagctcc ggtatttgcg caggtgatgg
     4141 cgggcgcgct gcgtgtgctc gacgtgccgc ccgacgggcc gatgcagccg atcgaactgc
     4201 cccccgaagg agaggaagtg aaggaaagcg tttaagtgtg cgagatcagg tcgaacacat
     4261 tcgggagcga ctcgctgcgc aaggggcgcg cgtgacgggc ctcgccgtgg aca
//
LOCUS       DASPZD010000074         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1531374, whole genome shotgun sequence.
ACCESSION   DASPZD010000074 DASPZD010000000
VERSION     DASPZD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531374"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1257
                     /locus_tag="VEM33_01405"
     CDS             64..1257
                     /locus_tag="VEM33_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYS50513.1"
                     /translation="MPYRSGRHFLQIPGPTNVPDRVLRAIDRATMDHRGPEFGPMGLE
                     ILEGLKKVFQTKAPVVIYPASGTGAWEAALVNTLSPGDRVLMFETGHFASLWHKMATK
                     LGLSVDLVPTDWRRGVDPAAVEAKLREDREHRIKAVCVVHNETSTGVASRIGPVRRAI
                     DSAEHPALYMVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNCVSEKALKA
                     SQSAKLPRAYWNWDEMLSNNRTGYFPYTPATNLLYGLRESLAMLEEEGLQNVFARHAR
                     HAESTRRAVRAWGLEILCASPEEYSNSLTAVMMPAGHDADALRKIILEAYDMSLGTGL
                     GKLAGKVFRIGHLGDFNDLMLAGTLAGIEMGLALANVPHKKGGVLAALDHLAESARAA
                     PRKAA"
     gene            1322..>2184
                     /locus_tag="VEM33_01410"
     CDS             1322..>2184
                     /locus_tag="VEM33_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50514.1"
                     /translation="MKFGKCSTLALLALTAVPALAWEPSKPVEIVVAAGAGGASDQMA
                     RMMQAAIQKNSLMKQPMVVSLKGGASGAEALIYMKSGAGDAHKFLIAYSLIYMLPLSA
                     KIPFNWRELTPVSIIALDQFVLWDNTTLPHKTVKEFIAAARNANPPFKMGGTGSKRED
                     HVLTVFLEKRTGAKFAYLPYKSGGEAATQLVGNHTQSNVNNPSENLEVWRAGQVRALC
                     VFDKERIQYKTKVTDAQSWNDIPTCKDEGVDVQYLMLRAMFLPGKVAPDQTAFYVDLF
                     RKLTQTSEYKD"
BASE COUNT          415 a          718 c          686 g          365 t
ORIGIN      
        1 atcatggagg ccgggagctt ttccgataaa ctagccgttc gtcgacgaaa agcgaacaac
       61 gttatgccct accgcagcgg acgccatttc ctgcagatcc ccgggccgac caacgtgccc
      121 gaccgggttc tgcgcgcgat cgaccgcgct acgatggacc atcgcggccc cgagttcggc
      181 cccatggggc ttgagatcct cgaaggcctg aagaaagtgt tccagaccaa ggcgcccgtg
      241 gtgatctatc ccgcctcggg caccggcgcc tgggaagcgg cgctggtcaa tacgctctct
      301 cccggcgacc gcgttctgat gttcgaaacc ggccattttg cctctctgtg gcacaagatg
      361 gcgaccaagc tcggcctctc ggtcgatctc gtccccaccg actggcgccg cggcgtcgac
      421 ccggcggcgg tcgaagccaa gctcagggaa gaccgcgagc accggatcaa ggccgtatgc
      481 gtggtgcaca acgagacttc gaccggggtc gcgagccgca tcggacccgt acgccgcgcg
      541 atcgactcgg cagaacaccc cgcgctctac atggtcgaca cgatttcctc gctcgcctcg
      601 atcgactacc gccacgacga gtggggcgtg gacgtgaccg tcgccggctc gcagaagggc
      661 ctgatgctgc ctcccggcct ctccttcaac tgcgtcagcg aaaaggcgct caaggcgtcg
      721 cagtctgcga agctgccgcg agcgtactgg aactgggacg agatgctttc caacaacagg
      781 accggctact ttccctacac gccggcgacc aacctgctct acggcctgcg cgaatcgctc
      841 gcgatgctcg aggaggaggg cctgcaaaac gtcttcgccc gccacgcgcg ccacgcggag
      901 agcacgcggc gcgcggtgcg cgcctggggc ctggagatcc tgtgcgcgtc gcccgaggaa
      961 tactcgaact ctctcaccgc ggtgatgatg cccgccggcc acgacgccga cgcgctgcgc
     1021 aagatcatcc tcgaagccta cgacatgtcg ctcggtaccg gcttgggtaa gctcgcgggg
     1081 aaagtattcc ggatcgggca cctcggcgac ttcaacgatc tgatgctcgc gggcaccctc
     1141 gccggcatcg agatgggcct cgcgctggcg aacgttccac acaagaaagg cggggtgctc
     1201 gcggcgctcg atcaccttgc cgagagcgcg cgggcggcgc cgcgcaaggc ggcctgattt
     1261 ttttgcaacc gcacggcccg ttggactaaa aggcggcgga acttcttttt gaggaggatt
     1321 catgaaattc ggtaagtgca gcacgctcgc tttgttggcg ctgacggccg tacctgccct
     1381 cgcgtgggag ccttccaagc cggtcgaaat cgtggttgcc gcgggcgcgg gcggcgcttc
     1441 cgaccagatg gcgaggatga tgcaggccgc gatccagaag aacagcctga tgaagcagcc
     1501 gatggtcgtg tcgctcaagg gcggcgcgtc gggtgcggaa gcgctgatct acatgaagtc
     1561 cggcgccggc gacgcgcaca agttcctgat cgcctattcg ctcatctaca tgctgccgct
     1621 gtcggccaag attccgttca actggcgcga gctcacgccg gtgtccatca ttgcgctcga
     1681 ccagttcgtg ctgtgggaca acaccacgct gccgcacaag accgtgaagg aatttatcgc
     1741 ggctgccagg aatgccaatc ctccattcaa gatgggcggc acgggctcca agcgcgagga
     1801 ccatgtcctc accgtcttcc tcgagaagag gaccggcgcg aagttcgcct acctgccgta
     1861 caagtcgggc ggcgaggcgg cgacccagct ggtcggcaac cacacccagt cgaacgtcaa
     1921 caatccttcg gagaacctgg aagtctggcg cgccggccag gtgcgcgcgc tgtgtgtgtt
     1981 cgacaaggag cgcatccagt acaagaccaa ggtcacggac gcgcaatcct ggaacgacat
     2041 cccgacctgc aaggacgaag gcgtcgacgt gcagtacctc atgctgcgcg ccatgttcct
     2101 gccgggcaag gtggcgcccg accagaccgc gttctacgtc gacctgttca ggaagctcac
     2161 gcagacgtcg gaatacaagg acta
//
LOCUS       DASPZD010000075         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_84212, whole genome shotgun sequence.
ACCESSION   DASPZD010000075 DASPZD010000000
VERSION     DASPZD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84212"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VEM33_01415"
     CDS             <1..765
                     /locus_tag="VEM33_01415"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYS50515.1"
                     /translation="NYWFKKHGLAGTYVPLAIRPEHLSAALRGLHPLGFAGVNVTIPH
                     KQEAMKIVHEVDTLAKSIGAISCVIVRSDGSLAGTNNDCYGFIHAVRQEQPGWRADAG
                     PVVVIGAGGGSRAVCYGLAQEGAREIRVVNRTLARAKGIAQEFGGPIRALPWEQRHEV
                     LEGAAMVVNTTSCGMVGQPALDIELDKLPQSALVADIIYIPLETPFLAAARKHGNRTV
                     NGLGMLLNQGRPAWKAWFGIEPEVTAELRAIVEKTI"
     gene            complement(784..1734)
                     /gene="gcvA"
                     /locus_tag="VEM33_01420"
     CDS             complement(784..1734)
                     /gene="gcvA"
                     /locus_tag="VEM33_01420"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HYS50516.1"
                     /translation="MIYRLPPLNALRVFEAAARHLSFKEAANELSITQAAVSHQIKSL
                     EEYLGVELFRRAGRGVQLTEAARAGLPKLREGFESLAAAVEMIRERAEETELLITAPP
                     VFTARWLMPRLADFAKREPRVELRVFGSSKMVDAGALDSAALVGNLDLREDVSGVEIH
                     LGGGNYPGYRADHLFDVATVAVASPELARGDPPLEQPEDLARHVLLHDDAMDLVAHGS
                     AWQKWLEAAGVADRVDGSRGPHFSSNILSLEAASQKLGVALALKPLVDDDIESGKLCA
                     PFKVELKPQSAYYLVCPEVIAGRPAVAAFRKWLLEQAVGA"
BASE COUNT          375 a          725 c          718 g          349 t
ORIGIN      
        1 aactactggt tcaagaagca cggcctggcc ggaacctacg taccgctcgc gatccggccc
       61 gagcatctgt ccgcggcctt gcgcggactt catccgctgg gattcgcggg cgtgaacgtc
      121 accatccccc acaagcagga ggcgatgaag atagtccacg aagtcgacac gctcgccaaa
      181 agcatcggcg cgatcagctg cgtgatcgtc agatccgacg ggtcgctcgc cggaaccaac
      241 aacgattgct acggcttcat ccacgcggtg aggcaggagc agcccggctg gcgcgcggac
      301 gccgggcctg tcgtggtgat cggcgccggc gggggctcgc gagccgtgtg ctacggcctt
      361 gcgcaggagg gcgcaaggga gattcgcgtg gtcaaccgca cgcttgcccg cgccaagggc
      421 atcgcgcagg aattcggcgg accgatcagg gccctgccct gggagcaacg ccacgaagtt
      481 ctggaaggcg cggcgatggt ggtcaacacc acgagctgcg gcatggtcgg ccagccggcc
      541 ctggacatcg agctcgacaa gctgccgcag agcgcgctcg tcgccgacat catctacatt
      601 ccgctcgaga cgccgttcct cgccgcagcg cgcaagcacg ggaaccggac cgtgaacggc
      661 ctggggatgc tcctgaatca gggccggccg gcctggaagg cgtggttcgg catcgagccc
      721 gaggtgacgg cggagctgag agcgatcgtc gagaagacga tatgagcgcg caaaaatgcc
      781 agcctacgct ccgaccgcct gctccagcag ccatttgcga aacgcggcga cggccgggcg
      841 cccggcaatg acctccgggc acacgaggta atacgccgac tgcggcttca gttccacctt
      901 gaacggcgcg cacagcttcc ccgactcgat gtcgtcgtcg actaggggct tgagggcgag
      961 cgccacgccc agcttctgcg acgccgcctc gagcgacagg atgttcgagg aaaaatgcgg
     1021 cccgcggctg ccgtcgacgc ggtcggcaac ccccgcggcc tcgagccatt tctgccaggc
     1081 gctgccgtgg gccacgaggt ccatggcgtc gtcgtggagc agcacgtggc gcgcgaggtc
     1141 ttccggctgc tcgagcggcg gatcgccgcg cgcgagctcc gggctcgcca ccgcgactgt
     1201 cgcaacgtcg aacaggtggt cggcccgata gccggggtag ttgcctccgc ccagatggat
     1261 ctcgacgccg gacacgtcct ccctcagatc gagatttccg acgagagcgg cggaatcgag
     1321 cgcgccggca tcgaccatct tgctcgagcc gaatacgcgc aattcgacgc gcggctcgcg
     1381 cttcgcgaaa tcggccaggc gcggcatcag ccagcgcgcg gtgaacaccg gcggcgcagt
     1441 gatcagcagc tcggtctctt cggcgcgttc gcggatcatc tcgaccgccg cggcgagcga
     1501 ctcgaagcct tcgcgcagtt tcggcaggcc cgcgcgcgcc gcttccgtga gctgaacccc
     1561 gcgtcccgcg cggcggaaca gttccacgcc gagatactcc tcgaggctct tgatctggtg
     1621 gctcacggca gcctgggtga tggacagctc gttcgccgcc tccttgaacg agaggtggcg
     1681 cgcggcggcc tcgaataccc gcagtgcatt cagcgggggc aggcggtaaa tcacgggcgg
     1741 gatgagtttt tcttatgcga cagatgagaa ttcatcgttt gttgttcgac cttcccggga
     1801 ctatcttcta ggacatgatg aaagccaaca tgaaatccag aactgaattg agcgtacctg
     1861 aattgtacgt gccggcgcct gccctcttgc gcacaccctt ctggcgggcg gcctagcact
     1921 cgctgccgga aagcgtgcgc ccgcggtacc tcgcgtatat ccagcacgcg gagcgctggg
     1981 atctcgcgct cgatgggatc atcgggaccc tgtcgcgagc gaagggcgct ttgaccaggc
     2041 tgcttgacac gcccaggcgg ccgcgctcgg cgcactgacc gcttgcctcg tcgttcgcgc
     2101 gacctgactc ctcctcctcc tccttggtca gtagcgcgga cggcgggcat ctctacgccg
     2161 cggcgaa
//
LOCUS       DASPZD010000076         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_225248, whole genome shotgun sequence.
ACCESSION   DASPZD010000076 DASPZD010000000
VERSION     DASPZD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225248"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /gene="aat"
                     /locus_tag="VEM33_01425"
     CDS             <1..700
                     /gene="aat"
                     /locus_tag="VEM33_01425"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HYS50517.1"
                     /translation="ASEVLAWLERDERFPSAERALKDPNGLLCAGGDLSLERLLDAYR
                     HGIFPWYSGAEPVLWWSPDPRMVLYCDELKLSRSLAKSARNKGYRVTADQVFADVLLG
                     CSEPRKADPGTWLGKDMRKAYLALHRAGYAHSFETWRGKELVGGLYGVAIGRMFYGES
                     MFSRATDASKVALVTLVQELSSRGFPLIDCQMSTPLLASLGAREIPRRAFLRAVAALV
                     NYANPPGRWALVET"
     gene            687..1424
                     /locus_tag="VEM33_01430"
     CDS             687..1424
                     /locus_tag="VEM33_01430"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HYS50518.1"
                     /translation="MSRLNDLPHAVLQFYVTAPYPCSYLPDRMARSQVATPSHLINTE
                     LYGDLVKAGFRRSGIFTYRPHCDACRACVPVRIPVREFAAHRSQRRAWRQHGGLTAHP
                     QPLKFRLEHYALYLRYQSKRHSGGGMDNDSRDQYSHFLLQSRVDTRLIEFRDAGQLVM
                     VSIVDYLPDGLSSVYTFYEPDRRGASFGTYNVMWQIDSCRKLGLPYLYLGYWIRESPK
                     MAYKSKFEPIEGFMGGLWRRLGRRDAA"
     gene            1421..>2378
                     /locus_tag="VEM33_01435"
     CDS             1421..>2378
                     /locus_tag="VEM33_01435"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HYS50519.1"
                     /translation="MSLYALARPLLFSLDPETAHRVTLQLAGFLPYAAVPPCPVRALG
                     LQFPNPVGLAAGLDKNAEHIDALAKLGFGFIEVGTVTPRPQAGNPRPRLFRLPQAQAI
                     INRLGFNNVGVDAFVQNVARSRYRGILGINIGRNADTPPERAADDYEMCLDKVYSHAS
                     YVTINISSPNTRGLRDLQQKDQVENLLARLSTRRERLAKRHGKHVPLVLKVAPDLVPG
                     QVSGIANAVRRHGIEGVIATNTSVSREGVEGLPHANEAGGLSGKPLRERATRVLKLLE
                     SELRDEATLIGAGGIFTQSDAREKVAAGASLVQLYTGLIYRGP"
BASE COUNT          389 a          814 c          774 g          401 t
ORIGIN      
        1 agcaagtgaa gtgctagcgt ggctcgagcg cgatgagcgc tttccgtccg ccgaacgggc
       61 gctgaaggac ccgaacggcc tgctgtgcgc cggcggcgat ctgtcgctcg agcgcctgct
      121 cgatgcctac cgccacggca tctttccctg gtactcgggc gcggagccgg tgctgtggtg
      181 gtcgcccgat ccgcgcatgg tgctgtactg cgacgagctc aagctgtcgc gctctctcgc
      241 caagagcgcg cgcaacaagg gctaccgcgt caccgcggac caggtgttcg cggacgtgct
      301 gctggggtgc tcggaaccga gaaaagctga tcccggaacc tggctcggca aggacatgcg
      361 caaggcctac ctcgcgctgc accgcgcagg ctacgcccac tccttcgaga cctggcgggg
      421 caaggagctg gtcggcggcc tgtacggggt ggcgatcggg cgcatgttct acggggaatc
      481 gatgttttcg cgggcgaccg acgcgtcgaa ggtggcattg gtcacactgg tgcaagagtt
      541 gagctcgcgt ggctttccgc tcatcgactg ccagatgagc acgccgctgc tcgcctccct
      601 cggagcgcgg gagatcccgc gccgggcctt cctgcgcgcg gttgcagcgc tggtaaacta
      661 cgcgaatccg cccggaaggt gggcccttgt cgagacttaa cgatctgccg catgccgtcc
      721 tgcagttcta cgtgacggcg ccctatccct gcagttacct gccggaccgc atggcgcgtt
      781 cccaggtggc gacgccgagc cacctgatca acaccgagct ttacggcgac ctggtcaagg
      841 ccggcttccg ccgcagcggc atcttcacct accggccgca ctgcgacgcc tgccgcgcct
      901 gcgtgccggt gcgcatcccg gtccgcgagt tcgccgccca ccgctcgcag cgccgcgcct
      961 ggaggcagca tggcgggctg actgcgcatc cgcagccgct caagttccgc ctcgagcatt
     1021 acgcgctgta cctgcgctac cagtcgaagc gccattccgg cggcggcatg gacaacgaca
     1081 gccgcgacca gtattcgcac ttcctgctgc agagccgggt cgatacgcgg ctgatcgaat
     1141 tccgcgacgc cggccagctg gtgatggtct cgatcgtcga ctacctgccc gacgggctgt
     1201 cctcggttta taccttctat gagcccgacc ggcgcggcgc aagtttcggc acctacaacg
     1261 tgatgtggca gatcgattcc tgccgcaagc tgggcctgcc ctacctgtac ctcggctact
     1321 ggatccggga gagcccgaag atggcgtaca agtccaagtt cgagccgatc gagggcttca
     1381 tgggcggcct gtggcgccgc ctcgggaggc gggacgcggc gtgagccttt acgctctggc
     1441 gcgcccgctc ctcttctcgc tagatcccga aaccgcgcat cgcgtcaccc tgcagctggc
     1501 gggatttctt ccgtatgccg cagtcccgcc ctgcccggtg cgggcgctcg ggctgcaatt
     1561 tccgaatccg gtcggcctgg cagccggcct cgacaagaac gcggagcaca tcgacgcgct
     1621 ggcgaagctg ggcttcggct tcatcgaggt cgggacggtg acgccgcgac cgcaggcggg
     1681 caaccccagg ccgcgcctgt tccggctgcc gcaggcgcag gcgatcatca accgcttggg
     1741 cttcaacaac gtcggcgtcg acgccttcgt gcagaacgtc gcgcgctcgc gctaccgcgg
     1801 catcctcggc atcaacatcg gcaggaacgc cgacacgccg cccgagcggg cggccgacga
     1861 ttacgagatg tgcctcgaca aggtgtattc gcacgcttcg tacgtcacga taaacatctc
     1921 ctcgcccaac acccggggcc tcagggatct gcagcagaag gaccaggtgg aaaatcttct
     1981 tgcgcgtcta tcgactcgga gagaacgtct cgccaagcgt cacggcaagc atgtcccgct
     2041 cgtgctgaag gtcgcgcccg acctcgtccc cgggcaggtg agcggaatcg cgaatgccgt
     2101 gcgccgccat ggcatcgagg gcgtcatcgc caccaatacc tcggtgtcgc gcgaaggcgt
     2161 cgaagggctg ccgcatgcga acgaagcggg cgggctgtcc ggaaaacccc tgcgggaacg
     2221 cgccacccgc gtgctcaagc tgctggagag cgaattgagg gacgaggcga cgctcatcgg
     2281 cgccggcggc atcttcactc agtccgatgc cagagaaaaa gttgctgcag gggcctcgct
     2341 ggtccagctc tacaccgggc tcatctaccg cggccccg
//
LOCUS       DASPZD010000077         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1648544, whole genome shotgun sequence.
ACCESSION   DASPZD010000077 DASPZD010000000
VERSION     DASPZD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648544"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="VEM33_01440"
     CDS             complement(<1..1549)
                     /locus_tag="VEM33_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HYS50520.1"
                     /translation="MKADALAGLIGAVVVLPQGVAFATLAGLPPEYGLYCAMVPTVVA
                     ALFGSSLHTVAGPTNPVSLMVFAVLSPLATPMTPHYIELALTLALMSGIIMLAMGLLR
                     FGSLVNFMSSSVVVGFTAALGVFIFASQLGNFLGIASAPAGFADLVLATLGHLGDAQP
                     WVIAVAATTLAIGALSRRMLPKVPPMLAAMVLGSLMAFLLNQILGVEQTGLRTLGPLP
                     GALPPLSFPDASAATLQNLLPGAVAVALVSLTQALSVAHAIALKSGQRLDNNQEFIAQ
                     GLANVAAAFFSGFPTSASANRCGINYDAGAKTPMSAIFSALLLVLVLLAVAPLVAYLP
                     IAVVAGLLFLVAWNLIDFQRIRKILATSRGESAVLAVTFFATLLLDLEFAILVGVLVS
                     LVLYLNRTSHPILRSLVPDPRHTGRKMTEVEDSLLECPQLKILRIEGSIYFGAVGPVQ
                     RHLDTLREHSPGQKHLLLMSKSINFVDMAGAELLVEEARRRRAGGGQLYFYSLRKPAE
                     ELLERGGY"
     gene            1733..>2652
                     /locus_tag="VEM33_01445"
     CDS             1733..>2652
                     /locus_tag="VEM33_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50521.1"
                     /translation="MPDFRKSSPTVMLVCASVILTLALGIRHGFGLFLQPMSMDNGWG
                     REVFGFAVALQNLLWGITQPFTGMLADRFGARRVLFAGGLLYALGLVLMANSTTALGL
                     TLAIGIFVGIGMSCTSFNIVFGALGRAYSAEKRSVVLGIASAAGSFGQFALLPLALVL
                     ITGFGWYSALIALSVLVAVVMPASFGVADKGYHGTLAAGAGVSLKEALAEAFRHTGFW
                     LLGVGYFACGFQVVFIGTHFPAFLMDKGLTVRDGTISLALIGLFNIFGSYLAGQLGGR
                     FSKTYLLSGLYAARGVAIALLLAFPLTPVSA"
BASE COUNT          445 a          862 c          893 g          452 t
ORIGIN      
        1 tgtagccgcc gcgctcgagg agctcctccg ccggttttct caagctgtag aagtagagct
       61 gcccgccgcc cgctcgccgc ctgcgcgctt cctccacgag cagctcggcg cccgccatgt
      121 ccacgaaatt gatgctcttc gacatcagga gcaggtgttt ctggccggga ctgtgctcgc
      181 gcagcgtgtc gaggtgccgc tggacggggc ccaccgcgcc gaagtagatc gagccttcga
      241 tgcgcaggat cttgagctgg gggcactcga ggaggctgtc ctcgacttcc gtcatcttgc
      301 gcccggtatg gcgcggatcc ggcacgaggc tgcgcaggat cgggtgggat gtgcgattga
      361 ggtacaacac caacgagacg agcacgccca cgaggatcgc gaactcgagg tcgagcagga
      421 gcgtcgcaaa gaaggtgacc gcgagcaccg cgctctcgcc gcggctcgtg gcgaggattt
      481 tccggatgcg ctggaagtcg atcaggttcc aggcgacgag gaaaagcagc cccgcaacga
      541 ccgcgatcgg caagtaggcg acgagcggcg cgaccgcgag gagcacgagc accagcagca
      601 gcgcggaaaa tatcgccgac atcggcgtct tcgcgcccgc gtcgtagttg atgccgcaac
      661 ggttggcgga ggcgctcgtc ggaaagcccg agaagaaggc ggcggcgacg ttggcaaggc
      721 cctgcgcgat gaattcctgg ttgttgtcga ggcgctgtcc cgatttgagc gcgatggcgt
      781 gcgcgaccga gagcgcctgc gtcagcgaca ccagcgcgac ggcgactgcg cccggcagga
      841 gattttgcag ggtcgccgcc gaggcgtccg ggaacgagag cggcggcagc gcgccgggca
      901 gcggccccag ggtgcgcagg cccgtttgct ccacgcctag gatctggttg aggaggaagg
      961 ccataaggct tcccagaacc atcgcagcca gcatcggcgg gactttcggc agcatgcggc
     1021 gcgaaagcgc gccgatcgcg agcgtcgtcg ctgccacggc aatcacccac ggttgcgcgt
     1081 cgccgaggtg cccgagcgtc gcgagcacca gatcggcgaa tcccgccggg gccgacgcga
     1141 tgccgagaaa gttgccgagc tggctcgcga aaatgaacac gccgagcgcc gcggtgaagc
     1201 cgaccacgac cgagctcgac atgaaattca ccagcgaccc gaaccggaga aggcccatcg
     1261 ccagcatgat gattcccgac atcagagcga gcgtgagcgc gagctcgatg taatgcgggg
     1321 tcatcggcgt cgcgagcggg ctgagcaccg cgaacaccat gagcgagacc ggattggtcg
     1381 ggccggcgac ggtatgcagg gacgagccga acagcgccgc gacgacggtg ggcaccatcg
     1441 cgcagtacag accgtactcg ggagggagcc ccgcgagagt cgcgaacgct acgccttgcg
     1501 gcaataccac gacagcaccg atcaggcccg cgagcgcgtc ggctttcaaa gtcgagcggt
     1561 cgacccgcgg ccaccacctg aggagcggga acaggcgccg cgagagcgga gcggtcgaaa
     1621 cggtcgaagg cgtcacgtcg gggtgaaggc cggaatccct atggtatcaa acgccgtcgc
     1681 gatgctcctc gcgtgatatc ctgtatcgct tttcctccgt ccggaatcct tcatgcccga
     1741 tttccgcaag tccagtccga ctgtaatgct cgtgtgtgcg agcgtgatcc tcacgctcgc
     1801 gctcggcata cgccacggct tcgggctgtt cctgcaaccg atgagcatgg acaacggctg
     1861 gggccgcgag gtgttcggtt tcgccgtcgc cttgcagaat ctgctgtggg gcatcacgca
     1921 gcctttcacc ggaatgctcg cggatcgctt cggcgcgcgg cgcgtgcttt tcgcaggagg
     1981 gctgctttac gcgctcggtc tcgtgctgat ggcgaactcg accaccgcgc tgggcctgac
     2041 tctcgctatc ggcatcttcg tcggcatcgg catgagctgc accagcttca acatcgtatt
     2101 tggcgcactg ggccgggcct attccgccga gaagaggagc gtcgtgctcg gcatcgccag
     2161 cgccgcgggt tccttcggcc agttcgcact gctgcctctt gcgctcgtcc tgatcaccgg
     2221 cttcggctgg tattccgcgc tgatcgcgct gtctgtgctc gttgccgtgg tgatgccggc
     2281 ctcgttcggc gtggccgaca agggctacca cggcacgctc gccgccggcg cgggcgtgtc
     2341 gctgaaagaa gcgctcgccg aggctttccg ccacacgggc ttctggctcc tcggtgtggg
     2401 ctacttcgcc tgcggcttcc aggtcgtgtt catcggcacg cacttcccgg cgtttctcat
     2461 ggacaaagga ctgaccgtcc gcgacggaac catctcgctc gcgcttatcg gcctgttcaa
     2521 tattttcggc tcttacctcg ccgggcagct cggcggccgt ttctcgaaga cctacctgct
     2581 ctccggcctc tacgccgcgc gcggggtcgc gatcgcgctg ctgctcgcgt ttccgctcac
     2641 gcccgtgtcg gc
//
LOCUS       DASPZD010000078         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_562816, whole genome shotgun sequence.
ACCESSION   DASPZD010000078 DASPZD010000000
VERSION     DASPZD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562816"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VEM33_01450"
     CDS             complement(<1..879)
                     /locus_tag="VEM33_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYS50522.1"
                     /translation="MAKARRVAVTGLGLVNPFGGDLADFFGRMLRGESAIRLYTREDD
                     VLRPLSVPAVRCANFDADAALGRSLAYTMDRYSQLGTTAALSAWSDAGLPRTKDAPRN
                     DWGVSWGTALGGTLTFESGYIDLLRNGRERVPPLSVVLGMSNAAASHISIQLGLGASS
                     ATYSVACASSAVAIGEALPKIRSGEATLMVVGGSEAPLSYGVVRAWEAMRILALGDEA
                     SAVRACRPFSSDRQGLVLGEGAGAMILEDWDHAVRRGARIYAEFAGCGSTSDHDHLVR
                     PNVDGQTRAMRMAIQDG"
     gene            complement(880..1125)
                     /locus_tag="VEM33_01455"
     CDS             complement(880..1125)
                     /locus_tag="VEM33_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYS50523.1"
                     /translation="MKQDYLAVLSEFLLKRENIDPARVTPETSLEELKVDSLLLLELL
                     FEFEDRLGVKIPHDIPRPKTVGDLLGIVDKVTAGSGA"
     gene            1208..1954
                     /locus_tag="VEM33_01460"
     CDS             1208..1954
                     /locus_tag="VEM33_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYS50524.1"
                     /translation="MTSPSPGRPDSELLDANRHFYNALWADARLIEPERFNTWPLVHS
                     LLAPAQRRLEVAPGLRPRLPIEDTQFVDISAAALGKLRTRGGSGTLGSITALPYGNAG
                     FDLICAFDVIEHVDDDERALAELSRVAVYGAALITSVPLHPAQWSGFDAFVGHRRRYE
                     PAELLDKLARHGFAVERSAAFGMKPKSPRLVDLGMWFLIHQRAPAIRLYNRVMMPLSV
                     RFQKPLALAQGLIDTAEVAEVLLLCRRASN"
     gene            complement(1951..2403)
                     /gene="ssb"
                     /locus_tag="VEM33_01465"
     CDS             complement(1951..2403)
                     /gene="ssb"
                     /locus_tag="VEM33_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYS50525.1"
                     /translation="MASVNKVILVGNLGRDPETRYAPEGGAITNISVATTDTWKDKTS
                     GEKQERTEWHRVVFFSRLAEIAGEYLKKGSQVYIEGSLRTRKWQDKEGKERTTTEIVA
                     DRMQMLGSRQGAGDAGAREAKEPAVAGEGKAAKKSEGKFDDMEDDIPF"
     gene            complement(2404..>2844)
                     /locus_tag="VEM33_01470"
     CDS             complement(2404..>2844)
                     /locus_tag="VEM33_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50526.1"
                     /translation="LLAFFVAFNILEASLPSLVSRLAPPASRGTALGVYNTTQALGLF
                     VGGAAGGWLAGHFGEASVFVFGIAIVALWLGTAWRMRIPGESAERIFPVRAGADPVAL
                     RGELVRLRGVREAVVVPEEGIARLTVYPESFDERAATEIIGGEN"
BASE COUNT          474 a          981 c          884 g          505 t
ORIGIN      
        1 cccatcctga atggccatgc gcatggcgcg cgtctgtcca tcgacattgg gccggacgag
       61 gtgatcatga tcggaggtgc tgccacaccc cgcgaattcg gcataaatcc tcgcaccgcg
      121 ccgaaccgcg tggtcccagt cctcgagtat catcgctccc gccccttctc cgagcaccag
      181 gccctgccga tccgaactga acgggcggca ggcgcgcacg gcggatgcct cgtcgccgag
      241 ggcaagaatt cgcatcgctt cccaagcgcg taccacgccg taggacagcg gcgcttcgga
      301 gccgccgacg accatcagcg tcgcttcgcc cgagcggatc ttcggcaagg cctcgccgat
      361 cgcgaccgcc gacgaggcgc acgcgaccga ataggtcgcg gaggaagccc ccaggccgag
      421 ctgaatcgat atatgggagg ctgccgcgtt gctcattccg agcacgaccg aaaggggcgg
      481 cacccgctcg cggccgttgc gcaacaagtc gatatagccg ctctcgaagg tcagcgtgcc
      541 gcccagcgcc gtgccccagg agacgcccca gtcgttgcgc ggcgcatcct tggtccgggg
      601 aagcccggcg tcgctccatg ccgaaagcgc tgccgtagtc ccgagctggc tgtagcggtc
      661 catggtgtag gcaagcgatc ggcccagcgc cgcatcggcg tcgaaattcg cgcaacggac
      721 cgccgggact gaaagcggcc tgagcacgtc gtcctcgcgg gtgtagagcc ggatcgcgga
      781 ctcgccgcgc agcatccggc cgaagaagtc cgcaagatcc ccgccgaaag ggttgactag
      841 gccgagaccg gtgacggcaa cgcgccgcgc ttttgccatt caagcgccgc ttccggccgt
      901 gaccttgtcc acgattccca gcaggtcacc gacggtcttg ggtcgcggaa tgtcgtgcgg
      961 gatcttgacg ccgagcctgt cctcgaattc gaacagaagt tcgagcagca gcagcgagtc
     1021 caccttgagc tcctcgagcg aggtctcggg cgtcactcga gccggatcga tgttctcgcg
     1081 cttgagcagg aattcactga ggacagccag gtagtcttgt ttcatgaatg aatagtgccg
     1141 cctcttggta acttcgcaac cgggtcagac ttccgcaatt ctcgcacgtc ctgtgttagc
     1201 gtggcgcatg acttcgccgt ccccgggccg ccccgattcc gaactgctcg atgccaaccg
     1261 gcatttctac aacgccctgt gggcggacgc acgcctgatc gagcccgaac gcttcaacac
     1321 ctggccgctg gtccactccc tgctcgctcc ggcgcagcgc cggctggagg tggcgccggg
     1381 attgcgcccg cgccttccga tcgaggatac gcagttcgtc gacataagcg cggcggcgct
     1441 gggaaaactg cgcacgcgag gcggctccgg gacgctgggc tccatcacgg cgttgcccta
     1501 cggcaatgcg gggttcgacc tgatctgcgc cttcgatgtg atcgaacacg tcgacgacga
     1561 cgagcgcgcc ctcgcggagc tctcgcgcgt ggcggtgtac ggggcggcgc tgatcacctc
     1621 ggtgccgctg catcccgcgc agtggtcggg gttcgacgct ttcgtcggcc accggcgccg
     1681 ctacgagcct gcagaactgt tggacaagct cgcacgacac ggcttcgcgg tagagcgcag
     1741 cgctgcgttc gggatgaagc cgaaatcccc gcgcctggtc gatctcggaa tgtggttttt
     1801 gatccaccag cgcgccccgg cgatacggct gtacaaccgc gtgatgatgc cgctgagcgt
     1861 gcgttttcag aaaccgctgg ccctggcgca aggcctgatc gacaccgcgg aggtcgcgga
     1921 agtcctcctg ttgtgccggc gcgcaagcaa ctagaacgga atgtcgtctt ccatgtcgtc
     1981 gaacttgccc tccgatttct tggcggcctt gccctcgccc gcgacggccg gctccttcgc
     2041 ttcccgggcg ccggcgtcgc ccgccccttg ccggctgccg agcatctgca tgcggtcggc
     2101 gacgatttcg gtggtcgtgc gttccttacc ctccttgtcc tgccacttgc gcgtgcgcaa
     2161 ggatccctcg atgtagacct gcgagccctt cttgaggtac tcgccggcga tttccgcgag
     2221 ccggctgaag aacaccacgc ggtgccattc ggtgcgttcc tgcttttcgc cgctcgtctt
     2281 gtccttccag gtatccgtgg tggcgaccga gatgttggtg atcgccccgc cctccggggc
     2341 gtagcgcgtt tcgggatcgc gccccaaatt gcctaccaaa atcactttgt tgaccgatgc
     2401 catttaattc tctcctccga taatttccgt cgccgcccgc tcgtcgaacg attcaggata
     2461 caccgttagt cgggcaatgc cttcttcggg caccaccacc gcttcgcgca cgccgcgcag
     2521 acgaacgagc tccccgcgca acgcgaccgg atcggcccct gcgcgcaccg ggaagatccg
     2581 ctctgcggat tcgcccggaa tgcgcattcg ccatgccgtt cccagccata acgcgacaat
     2641 cgcgattccg aatacaaaca ccgaggcttc gccgaaatgt ccggcgagcc agcccccggc
     2701 ggccccgccg acgaacagcc ccagcgcctg ggtggtgttg tacaccccga gcgcggtgcc
     2761 gcgcgaggcc ggcggcgcca gcctggatac cagcgaaggc agactcgcct ccagtatgtt
     2821 gaaggcgacg aagaaggcga gcag
//
LOCUS       DASPZD010000079         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1790609, whole genome shotgun sequence.
ACCESSION   DASPZD010000079 DASPZD010000000
VERSION     DASPZD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790609"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VEM33_01475"
     CDS             <1..365
                     /locus_tag="VEM33_01475"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50527.1"
                     /translation="EDLAPSQRVDLELPLAEVNTDLERLIRHLEPCGAGNPAPVFGVR
                     NARAGGARRVGTNHLRFTLDDGSGLLPAIGFRWADAVPQDWLTNPLDVAFRLERDDWQ
                     GRTSIQARVASLAPHHRG"
     gene            343..906
                     /gene="rsmD"
                     /locus_tag="VEM33_01480"
     CDS             343..906
                     /gene="rsmD"
                     /locus_tag="VEM33_01480"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HYS50528.1"
                     /translation="MLRIIAGEFKGRRLKAPTGRSVRPTADRVKEAWFSILQQSIPDA
                     RVLDLFAGSGALGFEALSRGAVSVDFVENSKASLAAIRDNATTLNVGDRVTIHRSDAL
                     CFAERLQPALYDVAFADPPYADEDAAQLVELFRVTPFARVFSIEHASDRSIGGDDTRK
                     YGDTAITFLYAPSPPFAALGRPSPPPL"
     gene            903..1382
                     /gene="coaD"
                     /locus_tag="VEM33_01485"
     CDS             903..1382
                     /gene="coaD"
                     /locus_tag="VEM33_01485"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYS50529.1"
                     /translation="MKRVAVYPGSFDPITKGHEDLIHRSLEFVDQLIVAVAVNVAKQP
                     LFSLEERVALIQQAVPDKRLAVQSFEGLLAEFAKRVGASVIIRGLRAVSDFEYEFQMA
                     LMNRNLAPKIETVFLVPAFDLTFVSSSLVREVARFGGDVSQLVDPTVQKALKRKFGK"
     gene            1379..2302
                     /locus_tag="VEM33_01490"
     CDS             1379..2302
                     /locus_tag="VEM33_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HYS50530.1"
                     /translation="MSFLDRVYERARKVKARIVLAEGEDARVQEAGRRIEREGLGAVR
                     LLERRTTHDAPRTMVALLRSRRPDKFPTDAAASQALEHPLTFGACLVGLGEADVMLGG
                     AMYTSAETIRAALSAVGTAPGIATVSGAFYMIKDDRVLTFADAAVTPEPTPSQLADIA
                     LVSARERRLIVGDEPVVAFLSFSTRGSAAGPRVQRIQDAVAALRRRLTSPPEFVFDGE
                     LQLDAALVPDVAARKAPGSPAAGGANVLIFPDLDAGNIGYKLVQRLGGWTALGPILQG
                     LARPISDLSRGATSDDIVNTAAIAILQTRKG"
     gene            2307..2642
                     /locus_tag="VEM33_01495"
     CDS             2307..2642
                     /locus_tag="VEM33_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYS50531.1"
                     /translation="MRFTLKKDKSGVVIVGVEGQLIVGNRQELKQKVLDALEEGSRKF
                     VIDFSKTGYIDSSGLGVLVSLSKKIREQGGELRLAGLNADLQTLFELTKLDTLFSITR
                     TAEEALAAF"
     gene            2639..3067
                     /locus_tag="VEM33_01500"
     CDS             2639..3067
                     /locus_tag="VEM33_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS50532.1"
                     /translation="MNLRLDVTPDSMKLDVPSDLGMVGDAVELVATHLPPGTLSPRRI
                     SFNFRTALAEALGNAIRYGNGEDPDRFVRVYVELGRDFVRIHVDDDGHGFDSSRVPDP
                     THPDNLEREYGRGLFVIRHLVDDVAFNEKGNGICLTLRAG"
BASE COUNT          573 a         1037 c         1114 g          591 t
ORIGIN      
        1 ccgaggacct ggcgccgagc cagcgcgtcg atctcgagct gccgctcgcc gaggtgaaca
       61 cggatttgga gcggctgatt cggcacctgg agccgtgcgg ggccggcaat cccgcgccgg
      121 tgttcggcgt gcggaacgcg cgggcgggcg gcgcgcggcg cgtggggacg aatcacctgc
      181 gcttcacgct cgacgacggc tcgggcctgc tgccggcgat cggctttcgc tgggccgacg
      241 cggtgccgca agactggctc acgaacccac tcgacgtcgc ctttcggctg gaacgggacg
      301 actggcaggg gaggacgtcg attcaggcga gagtggcgag tcttgctccg catcatcgcg
      361 ggtgagttca aggggcggcg cctaaaagcg ccgacgggga gaagtgtcag gccaactgcg
      421 gacagagtaa aagaggcctg gttcagcatt cttcaacagt cgattcctga tgcgcgcgtg
      481 ctggatctgt tcgctgggtc aggggcgctg ggatttgaag cgctgtcgcg aggcgcggtt
      541 tccgtcgact ttgtggagaa cagcaaagca tcactagctg cgattcgtga caacgcaacg
      601 accctcaacg taggggaccg cgtgacgatt caccgaagtg atgcgctgtg cttcgccgaa
      661 cgactccaac ccgcgttgta cgacgtcgca ttcgcagacc caccatatgc cgatgaggat
      721 gccgcacagc tcgtggagct ctttcgggtc acgccgttcg cgcgggtgtt ctcgatcgag
      781 catgcgtcgg atcggagtat tggtggcgac gacacgcgga aatacggcga cacggcgatc
      841 acatttctgt acgcgccttc gccgcccttc gccgcccttg gccgcccttc gccgccgcct
      901 ctatgaagcg cgtcgcggtc taccccggct ccttcgatcc aatcaccaag ggtcacgaag
      961 acctgattca ccggtcgctg gagtttgtcg atcagctgat cgtggcggtc gcggtaaacg
     1021 tcgcgaagca gccgctcttt tccctcgagg agcgcgtcgc gctgatccaa caagcagtac
     1081 ctgataagcg cttggcagtg caatcgttcg aagggttgct cgccgagttc gcgaaaaggg
     1141 tcggtgcgtc ggtgatcatt cgtgggctgc gggcggtatc ggattttgag tatgaattcc
     1201 agatggccct gatgaaccgc aacctggcac ccaagatcga gaccgtcttc ctcgtgccgg
     1261 cgttcgacct gaccttcgta agctccagcc tggtgcgcga ggtcgcgcgc ttcggcggcg
     1321 acgtatccca gctcgtcgat cccaccgtgc agaaggcact caagcgcaag ttcgggaaat
     1381 gagcttcctg gaccgcgtct acgaacgggc gcggaaggtc aaggctcgca tcgtgctcgc
     1441 ggagggcgaa gacgcccgcg tgcaggaagc aggacgcagg atcgagcgcg aaggcttggg
     1501 agcggtccgg ctgctcgagc gacgcaccac gcacgacgca ccgcgcacga tggtagcgct
     1561 actgcggtct cgacgacctg acaagttccc gaccgacgcc gctgccagcc aggcactcga
     1621 acatccactg accttcggtg catgcctcgt ggggcttggc gaggccgacg tcatgctcgg
     1681 cggcgcgatg tacacgagcg ccgaaacgat ccgggctgcg ctatcggcag tgggcaccgc
     1741 gcccggtatc gccacggtgt ccggggcgtt ttacatgatc aaggacgacc gcgtgctcac
     1801 cttcgccgat gccgcggtga ccccggaacc gacgccgagc cagctggcgg acatcgcgct
     1861 ggtgtcagcg cgggagcggc gcctgatcgt gggagacgag cccgtcgtcg cctttctatc
     1921 ttttagcacg cgcgggagcg ctgcgggacc gcgggtgcag cggattcagg acgcagttgc
     1981 cgcccttcgc cgccgcctca cctcgccgcc cgaattcgtt tttgacggcg agctgcaact
     2041 cgacgcggcg ctcgtcccgg acgtggcggc acgcaaggcg ccgggctcac ccgccgccgg
     2101 tggcgccaac gtgctgatat tcccggatct cgacgccgga aacatcggct acaaactggt
     2161 gcagcggctc ggcggctgga ccgcgctcgg cccgattctg caaggtctcg cgcgccccat
     2221 ctcggacctg tcgcgcggcg ccacctccga tgatattgtg aacactgcgg cgatcgccat
     2281 tctccaaacc cggaaggggt aatgggatgc ggttcaccct gaagaaggac aagagcgggg
     2341 tcgtgatcgt gggggtggaa ggccagctca tcgtcggcaa ccgccaggag ctgaagcaaa
     2401 aagtcctcga cgcgctcgag gaaggctctc gcaagtttgt gattgatttt agtaagactg
     2461 ggtatatcga ttcgtccggg ctgggcgtgc tggtgtcgct gtcgaagaag attcgcgagc
     2521 agggcgggga gctgcggctc gccggtctca acgccgacct ccagacgctg ttcgagctca
     2581 ccaagctcga cacgctgttc tcgatcacgc ggaccgccga ggaggcgctc gccgcgtttt
     2641 gaacttacgc ctcgatgtga ccccggactc gatgaagctc gacgtcccga gcgatctcgg
     2701 gatggtgggc gacgccgtcg agttggtcgc gacgcatctt cctcccggta cgctgtcccc
     2761 gcgccgcatc agttttaatt tccgaacagc acttgcagaa gcgctcggga acgccattcg
     2821 ctatggcaac ggcgaggatc ccgatcgctt cgtgcgggtc tacgtggagc tgggtcgcga
     2881 cttcgtccgc attcacgtgg acgacgacgg ccacggcttc gactccagcc gggttcccga
     2941 tccgacgcat cccgacaatc tcgagcgcga gtacggacgc gggttgttcg tcattcgtca
     3001 cctcgtggat gacgtggcgt tcaacgagaa aggcaacggc atctgcctga cgctccgagc
     3061 ggggtgacac ggctcgagcg cctgttgccg gtactcgggg cgctttctgg gacaactctg
     3121 cggatattgc gtgccaagag ccgtccggag tcctccgacg ggcccgccgt gttccagccg
     3181 attcccacca ccagggacgc gtggcttgaa gtacgcggag gcggggagcg cgccgccgtc
     3241 gagctcgccg agatcgtcgg ggcggtgatg gaggcggaag aggaaacgac ccaggtcgcg
     3301 gcggagctgt cggga
//
LOCUS       DASPZD010000080         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_444905, whole genome shotgun sequence.
ACCESSION   DASPZD010000080 DASPZD010000000
VERSION     DASPZD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444905"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VEM33_01505"
     CDS             complement(<1..406)
                     /locus_tag="VEM33_01505"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HYS50533.1"
                     /translation="MRSKGVAMDENATSPQYVGFWKRVFATFIDTLILVVVIGLIAVA
                     VYGMQYVRLSSEGKTVMFDLLVQGLLPALAVIVFWRYRGATPGKMLISAKIADAETFG
                     TPSTGKLIGRYFAYIVSCIFMLGFIWIAFDKRK"
     gene            476..1447
                     /locus_tag="VEM33_01510"
     CDS             476..1447
                     /locus_tag="VEM33_01510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HYS50534.1"
                     /translation="MKAVLCKNWGPPDSLVVEDVPSPAPGKGQLLVSVKASGVNFPDV
                     LLIQNKYQFKPELPFSPGSEIAGVVRQVGEGVHGFMPGDRVMAHIRWGGYAEEAVVDE
                     ALVAPVPAGLDFTVAASFGLVYATAYHALRDRARIKAGETLLVLGAAGGVGLAAVELG
                     KLFGARVIACASSDEKLETCRRFGADVTINYEREDLREALRNLGGAVDIALDPVGGKH
                     SEPALRAMAWKGRYLVVGFASGEIPKIPLNLTLLKGCAIVGVFWGEFARRERDLNAAN
                     LRELTAWLRAGKIKPLVSASYPLARAADALNDMISRKVQGKVVLTTG"
     gene            complement(1458..1943)
                     /locus_tag="VEM33_01515"
     CDS             complement(1458..1943)
                     /locus_tag="VEM33_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50535.1"
                     /translation="MRRAIAILLGLALLASTDAVFARGGRGGAGHAGGGRGGPSMGFH
                     GGHGGIATVGAHVGHPIGRTFVGVRPGFVGRPYPPHHHHGFHGGTVVIGAPFFFPYYP
                     YYPYYPSAYPVPNAEPPVYIEQGAGVYYYCPDYNDYYPNVSSCPSQWVPVSASPSQAP
                     R"
BASE COUNT          393 a          678 c          711 g          346 t
ORIGIN      
        1 gcttgcgctt gtcgaaggcg atccagatga atccgagcat gaagatgcag gaaacgatat
       61 aggcaaagta acggccgatc agctttcccg tggaaggcgt cccgaatgtc tccgcatccg
      121 ctatcttggc cgagatcagc atcttcccgg gggtcgcgcc gcggtagcgc cagaaaacga
      181 tcaccgccag cgcagggagc agtccctgca cgagcaaatc gaacatcacg gttttcccct
      241 cggaggagag gcgcacgtat tgcatcccgt agaccgcaac cgcaatcagt ccgataacga
      301 cgacaaggat cagggtatcg atgaacgtgg cgaaaacccg cttccagaac cccacgtatt
      361 gcggcgatgt cgcgttctcg tccatggcca cccccttcga gcgcatggta gcccgaccct
      421 tgtgaccgag gcaaccgcgc agttatgatg gcccgggaat ccggaaaggg cgcgcatgaa
      481 agccgtgctg tgcaagaatt gggggccgcc cgactcgctg gtggtcgaag acgttccgtc
      541 gcccgcgccg ggcaagggac agcttttggt ctccgtgaaa gcgagcgggg tgaatttccc
      601 ggacgtcctc ctcatccaga acaagtacca gttcaagccc gagctgccct tctcgcccgg
      661 gagcgagatc gccggcgtgg tcaggcaggt cggcgagggc gtgcacggtt tcatgcccgg
      721 ggaccgggtg atggcgcaca tccgctgggg cggctatgcg gaggaagccg tggtcgacga
      781 agcgctcgtc gcgccggttc cggccggcct cgatttcacg gtcgccgcct cgttcggcct
      841 ggtctacgcg acggcctacc acgcgctcag ggaccgcgcg cgaatcaagg cgggcgagac
      901 gctgctcgtg ctcggcgcgg cgggcggggt cggccttgcc gccgtcgagc tcggcaagct
      961 cttcggtgcg cgcgtgatcg cctgcgcctc ctccgacgaa aagctcgaga cctgcagacg
     1021 cttcggcgcc gatgtcacga tcaactacga gcgcgaggac ttgcgcgagg cgctcaggaa
     1081 tctgggaggc gccgtcgaca tcgccttgga cccggtcggc ggaaagcatt ccgagcccgc
     1141 gctgcgcgca atggcctgga agggacgcta cctcgtggtc ggcttcgcct cgggcgagat
     1201 ccccaagatt ccgctcaacc tgacgctgct caagggctgc gctatcgtgg gcgttttctg
     1261 gggcgagttc gcgcggcggg aacgcgatct caacgcggcg aacctgaggg agctcactgc
     1321 gtggctacgc gcggggaaga tcaagccgct cgtgagcgcg agctacccgc tagcgcgcgc
     1381 agccgacgcg ctcaacgaca tgatcagccg caaggttcag ggaaaggtcg tgctgaccac
     1441 cggctagcgg cgggccttta gcgcggtgct tggctcgggc tcgctgagac cggcacccac
     1501 tgcgaggggc agctggagac gttggggtag taatcgttgt aatcggggca gtagtaatac
     1561 acgcccgcgc cctgctcgat gtagaccggc ggttccgcat tcgggaccgg gtacgccgaa
     1621 ggatagtaag gatagtaggg atagtaagga aaaaagaacg gcgcaccgat cacgacagtg
     1681 ccgccgtgga atccgtggtg gtgatgggga ggatagggac ggccgacgaa cccggggcga
     1741 acgcccacga aagtccgtcc gatgggatgc ccgacatgtg cgccgacggt ggctattccc
     1801 ccgtgtccgc cgtgaaagcc catcgatggc ccgccgcgtc ctccgcctgc atgacctgcg
     1861 ccgcccctgc cgccgcgcgc gaagacggcg tctgtgctcg ccagcagcgc caatcccagc
     1921 agaatcgcaa tcgctcgtcg cattggagcc tcctattcgc ggagaacccg gctccgaaga
     1981 agtgtaacgc cggcgccggg ccggcgctcg ccttttacac ctcttacacc tcgttacatc
     2041 cgggttcgct cagcactccg gctcgcgcgc ccggggcgct ggcctagcga cgaatccgaa
     2101 ccggccggga acgtccgcgg agtgaatg
//
LOCUS       DASPZD010000081         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_321079, whole genome shotgun sequence.
ACCESSION   DASPZD010000081 DASPZD010000000
VERSION     DASPZD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321079"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VEM33_01520"
     CDS             complement(<1..1183)
                     /locus_tag="VEM33_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYS50536.1"
                     /translation="MPNPAFVHLRLHSEFSITDGIVRIDEAVARAAADGMPALAITDL
                     ANVFGMVKFYKAARAAGVKPIVGCDVWISNESERDKPHRLLLLTQSRAGYLRLSELLT
                     RAWLENQYRGRAELRKAWFGEGTEGLIALSGAMQGDVGAALLQDHREPAKRLAMEWAR
                     LFPGRFYLELQRAGAPHAESYVEAAVRLAGSSSLPVVATHPVQFLKPADFTAHEARVC
                     IAEGQVLGDQRRPRPFTPESYFLSQTEMAKLFADLPEALANSVEIARRCNLALELGKP
                     RLPAFPTPPGVSREEYLRQQAQDGLERRLVANHGGRADEKWKEKYELRLEVELRTIIQ
                     MGFAGYFLIVADFINWAKSNGVPVGPGRGSGAGSLVAYSLGITDVDPLRYDLLFERFL
                     NP"
     gene            complement(1387..>2455)
                     /locus_tag="VEM33_01525"
     CDS             complement(1387..>2455)
                     /locus_tag="VEM33_01525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HYS50537.1"
                     /translation="WTAPDGRWYQVLLARLPLASAANGEPQSVLIALSYDRSLPRELL
                     ARYRKGLLETLIVSVLAAAALGIWAARLVLKRARRIAATAGRISARALNERLSLDDTP
                     EEFLESTLAFNHMLDRLEDSFKRLSEFSSDLAHDLRTPINNLLGEAQVALSRPREASE
                     YRAVLESAVEEYERLSRMLENMLFLARADNAQARAAPQWIDLREALGKILSYYELLAE
                     ERNIRLALEVRAQKGGRPRAWADELMLNRAVGNLLSNALRHGRPDCTVTVKAVAHGDG
                     SAEIEVANPGSGIAAEHLPRIFDRFYRPCSSREESSAGSGLGLAIVKSIAELHGGTVG
                     VRSEPGLQTTFTLSFPAAGHA"
BASE COUNT          408 a          825 c          830 g          392 t
ORIGIN      
        1 ccggattcag gaaacgctcg aacagaaggt cgtatcgcaa ggggtccacg tcggtgatgc
       61 cgagcgagta ggcgacgagc gacccggcgc ccgagccgcg tcccgggccg accggcacgc
      121 cgttcgattt cgcccagttg atgaagtcgg cgacgatgag gaagtagccg gcgaatccca
      181 tctggatgat ggttcgcagc tcgacttcca ggcgaagctc gtacttttcc ttccacttct
      241 cgtccgccct gcctccatga ttcgcaacga gccgccgctc tagcccatcc tgggcttgct
      301 gacgaagata ttcctcgcgc gacacgcccg gtggcgtcgg gaaggccggc aaccttggct
      361 tccccagctc gagcgcgaga ttgcagcgcc gcgcgatctc gacggagttg gcgagcgcct
      421 cgggcagatc cgcgaagagc ttcgccattt ccgtctgaga cagaaaataa ctctccggcg
      481 tgaagggccg agggcggcgc tggtcgccga gcacctggcc ttcggcgatg cacacgcgcg
      541 cttcgtgtgc ggtgaaatcc gcaggcttca ggaactgcac cgggtgggtc gcgaccaccg
      601 gtagggagga gcttcctgcg agccgcaccg cagcctcgac gtagctctcc gcgtgcggcg
      661 cgccggcgcg ctgcagctcg agatagaacc gcccgggaaa gagccgcgcc cactccattg
      721 cgagccgttt cgcaggctcc cggtgatcct gcaggagagc ggcaccgaca tcaccctgca
      781 tcgcaccgga gagggcgatc aggccctcgg tgccttcgcc gaaccaggcc ttgcgcagct
      841 cggcgcggcc ccggtattga ttctcgagcc aggcgcgcgt caggagctcg gacaacctga
      901 gatagcccgc gcgcgactgc gtgagcagca gcagccggtg cggcttgtcg cgctcggact
      961 cgttgctgat ccagacatcg cagccgacga tgggcttcac gcccgccgcg cgcgcggcct
     1021 tgtagaactt gaccatgccg aagacgttgg cgaggtcggt gatcgccagg gccggcatgc
     1081 cgtccgcggc ggctcgcgcc acggcttcgt cgatgcgcac gatcccgtcg gtgatcgaga
     1141 actcgctgtg gagccgaagg tgtacgaagg cgggattcgg catcggcttg cagggaacgg
     1201 gcgaggtgga aattttacca cccgctcccg cggcctacga cgcaagtttg gctgcgatcg
     1261 aaaaattgcg gcaggctccg cgttccgatg aggcagtcgt ctcgcgcgag gaattcatga
     1321 ccccgatcaa aggactagtc gcgctcgatc gcgattagcc tccttcggga tctcgcggcg
     1381 ctggagctag gcgtggccgg ccgcgggaaa gctgagcgtg aaagtggtct gcaggcccgg
     1441 ctcgcttctt acaccgaccg ttccgccatg cagttccgcg atcgacttga cgatcgccag
     1501 acccagaccc gacccggccg aggattcctc gcgcgagctg caggggcggt agaagcggtc
     1561 gaagatgcgc ggcaggtgct ccgccgcgat cccggagccc ggattggcca cctcgatctc
     1621 ggccgaaccg tcgccgtgcg ccaccgcttt cacggtgacc gtgcagtccg ggcggccgtg
     1681 gcgcagcgcg ttggagagaa gatttcccac cgcccggttg agcatcagct cgtccgccca
     1741 ggcgcgcggc ctcccgcctt tctgcgcgcg cacttcgagc gcgaggcgga tgtttctctc
     1801 ctccgcgagc agctcgtagt aggaaaggat ctttcccagc gcctcgcgca ggtcgatcca
     1861 ctgcggcgcg gcgcgcgcct gcgcgttgtc ggcgcgggcg agaaagagca tgttctcgag
     1921 catccgcgag agccgttcgt attcctcgac ggcggattcg agcaccgcgc ggtactcgga
     1981 agcctcccgc ggacgcgaca gcgccacctg cgcctcgccc agaaggttgt tgatcggcgt
     2041 gcgcaggtcg tgcgcgagat ccgaggagaa ctccgagagg cgcttgaacg aatcctcgag
     2101 ccgatcgagc atgtggttga acgcgagcgt cgattcgagg aactcctccg gcgtatcatc
     2161 cagcgagagc cgctcgttca acgcccgcgc gctgatccgg ccggcggtcg cggcgatgcg
     2221 ccgggcgcgc ttcagcacca ggcgcgcggc ccagatgccg agcgcggccg ccgccagcac
     2281 gctcacgatc agggtctcga gcagcccctt gcggtagcgc gcgagcaatt ccctcggcag
     2341 cgaacggtcg tacgagagcg cgatcagcac ggactgcggc tcgccgttcg cggcggaagc
     2401 gagcggcagg cgcgcgagca gcacctggta ccagcgcccg tcgggcgcgg tccac
//
LOCUS       DASPZD010000082         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_222985, whole genome shotgun sequence.
ACCESSION   DASPZD010000082 DASPZD010000000
VERSION     DASPZD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222985"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VEM33_01530"
     CDS             <1..896
                     /locus_tag="VEM33_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50538.1"
                     /translation="SPSYFPAIAAVELGYFDKEGLEATIELLFPVTKTYEALREGRLD
                     FVGGAAHAALYAFRDWAGCKLLCALSQNMYWFLVVRRDLGIGRGDLRALKGLRIGAAP
                     GPADGLKRMLVDSGIDPEREVDIAPVPAAAGVSFGLAAARALEKGVVDGFWANGMAAE
                     IALRGGFGTLVIDARRGDGPKSAQHHAFPALVTTQKTIDERPDAAAAAIRAVVRAQRA
                     LKNDPSLAAAAAKRHFPVAELALIPELVRRDAPYYDPAISREAVDSLNAFARASGLLS
                     RAPAYEQVVATQFQALWSGAP"
     gene            949..1296
                     /locus_tag="VEM33_01535"
     CDS             949..1296
                     /locus_tag="VEM33_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50539.1"
                     /translation="METIRQLKALWAYASWCAQAPASAPACNSFWTWTAVAVVTAFAL
                     IGLHIIRKMAKNFLAVRAERQRLAEASRVAEPDTMSRYRVDPEKFHPDPPQQEGIERR
                     IRQALDEKKLEQR"
     gene            1330..>2408
                     /locus_tag="VEM33_01540"
     CDS             1330..>2408
                     /locus_tag="VEM33_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50540.1"
                     /translation="MPVANRSILAGIGALFALGLAAQSALAVPSGPPVRIGSTLALTG
                     PLAATGLVHKIVGEIYVEQLNRKNGLLGRPVEWVLKDDQSRPDLARTLYEQLITVDKV
                     DLIMGPYATANILSAMGVAQRYNKVLIHHTFGTPSLAKYEMQFPAGGLAGDPDNTIPK
                     LVFDALAASGKPPKSIAVVTSKFPSVHFISLGAREAAKKRGLREAAFLEWDFGNRDFG
                     PIAARLKDAKPDFVWVGGLGLEGNLLIDAVRKIDYMPPLHFHLFPAPGPLLKAPGGEN
                     ALAATTFEEHPPFTDNPVAVEFIRLYHERGAKAGLPDTSVENQSAISYSSWQTLEAGV
                     TGAKSIDDKAIAQWLKKNPVDAIVG"
BASE COUNT          414 a          808 c          796 g          390 t
ORIGIN      
        1 tctcgccgtc gtactttccg gcgatcgccg cggtcgagct cggctacttc gacaaggagg
       61 gtctggaggc gacgatcgag cttctcttcc cggtcacgaa gacctatgaa gccctgcgcg
      121 aggggcggct cgacttcgtc ggcggcgcgg cgcacgcagc gctctacgcc ttccgcgact
      181 gggcgggatg caagctgctt tgcgcgctct cgcagaacat gtactggttc ctcgtcgtgc
      241 gcagggacct cggcatcggg cgcggcgatc tgcgcgccct gaaggggctg cgcatcggcg
      301 cggcgcccgg cccggcggac ggcttgaagc gcatgctcgt cgattccgga atcgacccgg
      361 agcgcgaggt cgacatcgcc cccgtgcccg cggccgcagg cgtgtccttc ggcctcgcag
      421 cagcgagagc gctcgaaaaa ggcgtggtgg acggtttctg ggcgaacggc atggccgcgg
      481 aaatcgcctt gcgcggcggc ttcggcacgc tcgttatcga cgcgcgccgc ggcgatggac
      541 cgaagagcgc gcagcaccac gcctttcctg cactcgtgac tacgcaaaag acgatcgatg
      601 aacgacccga cgcggcagcc gccgcgatcc gcgccgttgt gcgtgcgcag cgcgcgctga
      661 aaaacgatcc ttcgctcgcc gccgccgcgg cgaaaagaca tttcccggtg gcggagctcg
      721 cgcttatccc cgagctcgtc cggcgcgacg cgccgtacta cgacccggcg atttccaggg
      781 aggcggtcga ttcgctcaac gcctttgcgc gggcgagcgg gttgctctcg cgggcgccgg
      841 cctacgagca ggtcgtcgcg acgcagttcc aagccctgtg gagcggagcc ccttagccgc
      901 ggccctctcc ttgtggcggt gggcggcatt gcgtagactc ggtgcgcgat ggaaacgata
      961 cggcagctca aggccttgtg ggcctacgcg agctggtgcg ctcaggcgcc ggcgtccgcg
     1021 ccggcctgca attccttctg gacctggacg gccgtcgccg tcgtcacggc cttcgcgctg
     1081 atcggactgc atatcatccg gaagatggcg aaaaatttcc ttgcagttcg ggcggagagg
     1141 cagcggctcg ccgaagcgtc gcgcgtggcc gaaccggata ccatgtcgcg ataccgggtc
     1201 gatcccgaga aattccatcc cgaccccccg cagcaggaag gcatcgagcg acgcatcaga
     1261 caagcgctcg acgagaaaaa gttggaacag cgctgaggcc ggcgcgcaca ccgttagagg
     1321 aggggaacca tgccagtcgc caatcggtcg attctcgccg gaataggtgc gctgttcgcg
     1381 ctcggtctcg cggcgcagag cgcgctcgcg gtgccttcgg gtccgccggt ccggatcgga
     1441 agcacgcttg cgctcaccgg gccgctcgcc gcgaccggcc tcgtccacaa gatcgtgggc
     1501 gagatctatg tcgagcagct caaccggaaa aacggcctgc tcggccgccc cgtggagtgg
     1561 gtgctcaagg acgaccagtc gcggcccgac ctcgcgcgaa cgctctacga gcagctcatc
     1621 accgtcgaca aggtcgacct gatcatggga ccgtacgcga cggcgaacat tctctcggcg
     1681 atgggcgtcg cgcagcgcta caacaaggtg ctcatccacc acaccttcgg cacgccgagc
     1741 ctcgccaaat acgagatgca atttccggcc ggcgggctgg cgggcgatcc tgacaacacg
     1801 atacccaagc tcgtcttcga cgcgctcgcg gcatccggga agccaccgaa gtcgatcgcg
     1861 gtcgtcacca gcaaattccc ctcggtgcat ttcatttccc tgggtgcgcg cgaggcagca
     1921 aagaagcgcg gacttcgcga agcggctttt ctcgagtggg acttcggcaa ccgcgacttc
     1981 ggcccgatcg ctgcacgcct gaaagacgcg aaacccgact tcgtgtgggt cggcggcctc
     2041 ggcctggagg gcaacctgtt gatcgatgcg gtgcgcaaga tcgactacat gccgccgctg
     2101 catttccacc tgttccccgc gcccgggccg ctcttgaagg cgcctggcgg ggaaaacgcg
     2161 ctcgcggcga cgaccttcga ggagcatccg ccgttcaccg acaatcccgt cgcggtcgag
     2221 ttcatccggc tgtatcacga gcgcggtgcc aaggccggat tgcccgacac ctcggtcgag
     2281 aaccagtcgg ccatttcgta ttcctcctgg cagacgctcg aggcgggcgt gaccggggca
     2341 aagagcatcg acgacaaggc catcgcacaa tggctgaaga agaatccggt ggacgccatc
     2401 gtcggcag
//
LOCUS       DASPZD010000083         5867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_923109, whole genome shotgun sequence.
ACCESSION   DASPZD010000083 DASPZD010000000
VERSION     DASPZD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923109"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /gene="mnmA"
                     /locus_tag="VEM33_01545"
     CDS             complement(<1..1042)
                     /gene="mnmA"
                     /locus_tag="VEM33_01545"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYS50541.1"
                     /translation="MAGDRIVVGMSGGVDSSVAALLLKRRGFDVVGLFMKNWEEDDDD
                     EYCSTRQDLIDCASVAEVIGIELEAVNFSAEYKERVFAAFLAEYSAGRTPNPDVLCNA
                     EIKFKAFLDHAIALGAKHIATGHYARVRESAGRFELLKAVDSAKDQSYFLHRLTQAQL
                     ARVVFPLGELRKTEVRRIAREAGLPNFAKRGSTGICFIGERPFREFLNRYLPRNPGPM
                     RTPEGKTVGEHIGLSFYTIGQRRGVGIGGMKDSAGGAWYVCGKDLAKNELIVVQGHDH
                     PRLLSRKLRAEDLAWVSGAAPQPSIPYGAKTRYRQADAACRIDRMGDEDLEVEFSAPQ
                     WAVTPGQSVVLYR"
     gene            complement(1051..2262)
                     /locus_tag="VEM33_01550"
     CDS             complement(1051..2262)
                     /locus_tag="VEM33_01550"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYS50542.1"
                     /translation="MPRVSRRTENLGTENAFVVLAEVNELVRQGRDIISFCIGQPDFH
                     TPANVQDAAVRAVREGRHGYTPSAGIAELREAAARYMGAMRGLTIRPEDVVIGGGAKP
                     FIAYSILSTTDHGVGDEVIYPNPGFPIYESQIVANGAVPVPLHLREARNFAFDPAELE
                     AKITKKTRLVILNSPQNPTGGILAKKDLEAIAAILRRHPEVWVYADEIYSRLCYEGSF
                     ASIASLPGMFERTIISDGASKTWAMTGWRIGFASNPVLAPQFVRWVTNTDSCASHVSQ
                     WAAVEAINGPQDAAEKMRDSFLARRDLIVGLLNEAPGVSCKTPGGAFYAWPNVTEACR
                     MVGAADSEEFRKRLLNEAGVAVLADIHFGARVPGEGQHIRFSYAASKTAIEQGVARMD
                     EFIRRNQRRVA"
     gene            2355..3320
                     /locus_tag="VEM33_01555"
     CDS             2355..3320
                     /locus_tag="VEM33_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HYS50543.1"
                     /translation="MLINCAAYQDGKKVGDVAPEDISKYVSRPECFVWVAMKDPGPGE
                     LAAMQHEFDLHELAVEDARHGHQRPKIEEYGDSLFAVLHNIEFVGGEIRVGEVDIFVG
                     RNFVLSVRNNAEPGFAAVRARCEREPELLKHGAGFVFYALIDAVVDRYFPVLDAIENE
                     FEKFETQIFSGKSLRANIEGLYYVRQKLTTLQHAVLPLLEAVGKLYGGRVPQVCSGLQ
                     DYYRDVYDHLVRINQNINSQRDTVTTAIQVNVAMITFGETEVTKRLAAWGALIAVPTF
                     IASVYGMNFVQMPELRWTFGYPMALGMMTAINVYLWFRFRKAGWL"
     gene            complement(3353..4801)
                     /locus_tag="VEM33_01560"
     CDS             complement(3353..4801)
                     /locus_tag="VEM33_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HYS50544.1"
                     /translation="MQVQSMHFKARSGEALADATLQANLRKFQSTGFTALRAKAVAEF
                     GPELFEALRGEGAAIRDRSLASLDAWLERFEAEAVRRGATVLYAETREEACELLLGIC
                     RRHGVKKAIKSKSMLSEEAGVNEALEANGVTPIETDLGEYILQLAGEPPSHIIAPALH
                     KSKEEVADLFETHHHAPRKDDIPHMTREAREVLREHFLSADLGISGGNFLIAETGSGV
                     IVTNEGNGRMVTTLPRVHVCITGIEKVLPTLEDFSTLLRLLTRSATGQAISNYVSVFT
                     GPRGRQDRDGPEHMVFILVDAGRTNLVGGDMQEMLRCIRCGACMNHCPVYRAVGGHSY
                     GWVYPGPMGSVLTPSYAGLENALDLPHAATLCGACSVACPVKIPLPELLRKLREKQMD
                     RGLRPWREVAAVRLWSWTARRPRLYALATAIAVRFLRLLGGRDGMIATLPFGREWTRG
                     RFFPAPKSGRTFRSLYAARNRGQTTVFRSKQR"
     gene            complement(4807..5538)
                     /locus_tag="VEM33_01565"
     CDS             complement(4807..5538)
                     /locus_tag="VEM33_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HYS50545.1"
                     /translation="MRVGLFVTCLVDLMRPSIGLAALKLLEDAGYEVTVPAAQTCCGQ
                     PGWNSGDRKGARSLAERLIAQFEECRYVVVPSGSCAGMIRAHFPGVFAGEPDMLAKAR
                     ELAGRTYELTEFLTKIAKRAGVSATFDGTVTYHDSCSGLREIGVKTQPRQLLANVAGL
                     KLIEMEECETCCGFGGTFSIKYGDISSAMAEKKCANIEASGAQAVVLGDLGCMLNIEG
                     RLRRRGDAKTRVLHVAEVLAGPTEG"
     gene            5620..>5867
                     /locus_tag="VEM33_01570"
     CDS             5620..>5867
                     /locus_tag="VEM33_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50546.1"
                     /translation="MPWLRIASFDIPQRPVPNTKSKAPSMIIDVDSHLREGYFMDEVY
                     KLDGPYERFRPVKLNDGRTHNTKFLHSLDPISPQGHAA"
BASE COUNT         1017 a         1967 c         1876 g         1007 t
ORIGIN      
        1 cgcggtagag aacgaccgac tggccgggcg tcaccgccca ttgcggcgct gagaattcca
       61 cctcgaggtc ctcgtcgccc atgcggtcga tgcggcacgc ggcatcggcc tggcggtagc
      121 gcgtcttcgc gccatacggg atgctcggct gcggcgcggc gccgctcacc caggcgagat
      181 cctctgcgcg aagcttccgc gacagcagcc gcgggtggtc gtgcccctgc acgacgatca
      241 attcgttctt cgccagatcc ttgccgcaca cgtaccaggc tccgccagcc gaatccttca
      301 tgccgccgat gccgacgcct cttctctggc cgatggtata gaaagagagc ccgatatgct
      361 ccccgacggt cttgccctcg ggcgtgcgca tcggaccggg attcctcggc agatagcggt
      421 tcaggaactc gcgaaaaggc cgctctccga tgaagcagat tccggtcgag ccgcgctttg
      481 cgaagttggg cagcccggcc tccctggcga tcctgcgcac ttcggtcttc ctcagctccc
      541 cgaggggaaa caccacgcgg gcgagctggg cctgcgtgag gcgatgcagg aaatagctct
      601 ggtccttggc ggaatcgacc gccttgagca gctcgaacct tcccgccgat tccctcacgc
      661 gcgcatagtg cccggtggcg atgtgcttcg cgcccagcgc gatggcgtga tcgaggaagg
      721 ctttgaactt gatttcggca ttgcacagga cgtcggggtt gggcgtgcgg ccggcggaat
      781 attccgcgag gaaagccgca aagacgcgct ccttgtattc ggccgagaaa ttcaccgcct
      841 cgagctcgat gccgatcacc tcggcgacgg aggcacaatc gatcaagtcc tgccgcgtcg
      901 agcagtactc gtcatcgtcg tcctcctccc agttcttcat gaagaggccg accacgtcga
      961 agccccggcg cttcaggagc agcgcggcga ccgacgaatc cacgccgccc gacatgccta
     1021 cgactatccg gtcgcccgcc atcgacggac tcaggccacg cggcgctggt tcctgcggat
     1081 gaactcgtcc atgcgcgcga cgccctgctc gatcgcggtt ttcgaagcgg cgtaggagaa
     1141 gcggatgtgc tggccctctc cgggcacgcg cgcgccgaaa tggatgtcgg caagcaccgc
     1201 gaccccggcc tcgttgagca ggcgcttcct gaactcttcc gaatccgcgg cgccgaccat
     1261 gcggcaggcc tcggtcacgt tcggccaggc gtagaaggcg ccgcccggcg tcttgcagga
     1321 gacgcccggg gcttcgttca gcaaacccac gatcagatcg cgccgcgcga ggaacgagtc
     1381 gcgcatcttt tccgcagcgt cctggggacc gttgatcgcc tcgaccgcgg cccactgact
     1441 cacgtgtgaa gcgcaggaat cggtgttcgt cacccagcgc acgaactgcg gcgcgaggac
     1501 ggggttcgag gcgaagccga tgcgccaccc ggtcatcgcc caggtcttcg aagcgccgtc
     1561 ggagatgatc gtgcgttcga acatcccggg aagcgacgct atcgacgcga acgatccttc
     1621 gtagcacagc cgcgagtaga tctcgtcggc atagacccag acttcagggt gccgcctcag
     1681 gatggccgcg atggcttcga gatccttttt cgccaggatg ccgccggtcg gattctgcgg
     1741 cgagttgagg atgacgagcc ttgtcttctt cgtgatcttc gcttcgagct cggccggatc
     1801 gaaggcgaaa ttgcgcgcct cgcgcagatg cagcggcacc ggcaccgcgc cgtttgcaac
     1861 gatctgcgac tcgtagatcg gaaacccggg attcggatag atcacctcgt cgcccacccc
     1921 gtgatccgtg gtcgaaagaa tggagtaagc gataaagggc ttcgccccgc cgccgatcac
     1981 gacgtcctcg gggcggatgg tcagcccgcg cattgcgccc atgtagcgag cagcggcctc
     2041 gcgcagctcg gcgattccgg ccgaaggcgt gtagccgtgc cggccttcgc gcaccgcccg
     2101 cacggccgca tcctggacgt tcgccggcgt atggaaatcc ggctgcccga tgcagaacga
     2161 gatgatgtcc ctgccctggc ggacgagctc gttgacttcc gcgaggacga cgaaggcgtt
     2221 ctcggtgcct aggttttcgg tgcggcggct gacgcgtggc atggacggaa gtcaggcgga
     2281 tgcaagggac gccattctag cctatgcgcc ggcgccttcg tgtcctatca ttgggccgta
     2341 tacgggggac tgggatgctg atcaactgcg cggcctatca ggacggcaag aaggtcggcg
     2401 acgtcgcgcc cgaggacatc agcaagtacg tcagccgccc cgaatgcttc gtgtgggtcg
     2461 cgatgaagga ccccgggccg ggcgagctcg ccgccatgca gcacgagttc gatcttcacg
     2521 agctcgcggt cgaggacgcg cgccacggcc accagcgccc caagatcgag gagtacgggg
     2581 actcgctgtt cgcggtgctg cacaacatcg agttcgtcgg cggcgagatc agagtcgggg
     2641 aggtcgacat cttcgtcggc aggaatttcg tgctgtcggt gcgcaacaat gccgagccgg
     2701 gcttcgccgc agtgcgcgcg cgctgcgagc gcgagcccga gctcttgaag cacggcgcgg
     2761 gcttcgtgtt ctacgccctg atcgacgcag tcgtcgaccg gtattttccg gtgctcgacg
     2821 cgatcgagaa cgagttcgaa aagttcgaga cacagatctt ctccggcaag tccctgcgcg
     2881 ccaacatcga agggctctac tacgtgaggc agaagctcac cacgctgcag cacgcggtgc
     2941 tgccgctgct cgaggcggtc ggcaagctgt acggaggccg cgtcccgcaa gtgtgctcgg
     3001 ggctgcagga ttactaccgg gacgtctacg accacctggt ccggatcaac cagaacatca
     3061 acagccagcg cgacaccgtg accaccgcga tccaggtgaa cgtcgccatg atcaccttcg
     3121 gcgagaccga agtgaccaag cgcctcgcgg cctggggcgc gctcatcgcg gtgccgacct
     3181 tcatcgccag cgtctacggc atgaacttcg tgcagatgcc ggaactgaga tggacgttcg
     3241 gctatcccat ggcgctcggc atgatgaccg cgatcaacgt ctatctctgg ttccgcttcc
     3301 gcaaggctgg ctggttgtga ctcagcagag aaaccgggga cagaccacgg ttttaccgct
     3361 gttttgacct gaaaaccgtg gtctgtcccc ggtttctcgc ggcgtagagg gaccggaagg
     3421 tcctgccgga cttcggcgcc gggaaaaacc ggccgcgggt ccactcccga ccgaagggca
     3481 gcgtggctat catcccgtca cgtccgccga ggaggcgcaa gaaacgcacg gcgatcgccg
     3541 tcgcgagcgc gtacaggcgc ggacgcctcg ccgtccacga ccacaggcgg accgccgcca
     3601 cttcgcgcca cgggcgcaag cctcgatcca tctgtttttc gcggagcttg cgcagcagct
     3661 cgggcagcgg gatcttgacg ggacacgcca ccgagcacgc gccgcaaagc gtcgccgcgt
     3721 ggggaagatc gagcgcgttt tccaggccgg cgtagctggg tgtgagcacc gagcccatgg
     3781 ggccggggta gacccagccg tagctgtgtc ctccgaccgc ccggtatacg ggacagtggt
     3841 tcatgcaggc cccgcaccgg atgcagcgca gcatctcctg catgtcgccg cccacgagat
     3901 tggtgcggcc ggcgtcgacc aggatgaaga ccatgtgctc ggggccgtcg cgatcctgcc
     3961 gtccgcgcgg accggtgaac acgctcacgt agttcgagat cgcctggccg gtcgccgagc
     4021 gcgtcagcag cctgagcagg gtcgagaaat cctccagcgt cgggagcacc ttctcgatgc
     4081 cggtgatgca cacgtgcacg cgcggcagcg tcgtcaccat gcgtccgttg ccttcgttcg
     4141 tgacgatcac gcccgacccg gtctcggcga tcagaaagtt gccgcccgag atgccgagat
     4201 cggcagagag gaaatgctcc cgcagcacct cgcgcgcttc gcgggtcatg tgcgggatgt
     4261 cgtccttgcg cggcgcatgg tgatgggtct cgaacagatc ggcgacctcc tctttgctct
     4321 tgtgaagcgc cggggcgatg atgtgcgaag gcggctcccc ggcgagctgc aggatgtatt
     4381 cgccgagatc cgtctcgatg ggcgtgactc cgtttgcctc gagcgcctcg ttcacccccg
     4441 cctcttcgga gagcatcgac ttcgacttga tcgccttctt gacgccgtgg cgccggcaga
     4501 tcccgagcaa gagctcgcac gcctcttcac gagtctccgc gtagagcacc gtcgcgccgc
     4561 ggcgcacggc ttctgcttcg aagcgctcga gccacgcatc caagctcgcg agcgagcggt
     4621 cgcggatcgc cgcgccctcc ccgcgcagcg cttcgaagag ctccgggccg aattcggcca
     4681 ccgccttggc gcggagcgcc gtgaaccccg tggactggaa cttgcgaagg ttcgcctgca
     4741 gggtcgcgtc ggcgagcgcc tccccggacc tcgccttgaa gtgcatcgat tggacctgca
     4801 aaggcgctac ccctcggtcg gcccggcgag cacttcggcg acgtgcagca cccgcgtctt
     4861 cgcgtctccg cggcggcgca gccgcccttc gatgttcagc atgcacccca ggtcgccgag
     4921 tacgaccgcc tgcgcgccgc tcgcctcgat gtttgcgcac tttttctccg ccatcgccga
     4981 ggagatatcg ccgtacttga tggaaaaagt gccgccgaag ccgcagcagg tctcgcactc
     5041 ttccatctcg atgagcttga gccccgctac gttcgcgagc agctggcgcg gctgcgtctt
     5101 cacgccgatc tccctcaagc ccgagcacga atcgtggtag gtcacggtgc cgtcgaaggt
     5161 cgcggaaacg cccgcgcgct tcgcaatctt ggtcaggaat tccgtgagct cgtacgtgcg
     5221 cccggccagc tcccgcgcct tcgcgagcat gtcgggctcg cctgcgaaga ctccggggaa
     5281 atgcgcacgg atcattccgg cgcacgaccc cgaaggcacg accacgtagc ggcattcctc
     5341 gaactgggcg atcagtcttt cggcgagcga gcgcgcccct ttccggtcgc cggagttcca
     5401 cccgggctgc ccgcagcagg tctgcgccgc cggcaccgtc acctcgtatc cggcgtcctc
     5461 gagcaacttg agcgcagcca ggccgataga cggccgcatc agatccacga ggcaggttac
     5521 gaacaagccg acgcgcatga aaattcggga aaacaggttg aacgggtggc gccgaaatca
     5581 tagcacgcgg gactcgggcg ccttgggagc gcgcgcccca tgccgtggct tagaattgcg
     5641 tcattcgaca tcccgcaacg acctgttccc aacaccaaat ccaaggcccc cagcatgatc
     5701 atcgacgtcg attcgcatct gcgcgaaggc tatttcatgg acgaggtcta caagctcgac
     5761 ggtccgtacg agcgtttcag gccggtcaag ctgaacgacg ggcgcacgca caacacgaag
     5821 ttcctgcatt cgctcgatcc catcagcccg cagggccacg cggcgca
//
LOCUS       DASPZD010000084         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1289649, whole genome shotgun sequence.
ACCESSION   DASPZD010000084 DASPZD010000000
VERSION     DASPZD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289649"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..372)
                     /locus_tag="VEM33_01575"
     CDS             complement(7..372)
                     /locus_tag="VEM33_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50547.1"
                     /translation="MIDWEPISKKALLKRIGQGYARMPQENQRLWESIRIDPEKWEQH
                     PYGDAGGGFWVVALIGRTVIWYNDLEDGFNRSVYSRYGVINDYWCNEDELYVTVQYLA
                     NALTGGKDLALVGLRKKRA"
     gene            complement(455..1096)
                     /locus_tag="VEM33_01580"
     CDS             complement(455..1096)
                     /locus_tag="VEM33_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HYS50548.1"
                     /translation="MRCEHSDELPDGRLVVSSAERNSGPILKVLERVLPKTGLVLEVA
                     SGTGQHVVHFARALKRLIWQPTDMEAGCRRSISAWLATANLANVRQPLDLDVRTLPWP
                     VPTLDAIVCLNLIHIAPWSVTTALFAGAGLALPEAGLLYLYGPYSMAGRHTAPSNAAF
                     ESALRAQNPEWGLRDLEEVASVAKEQGFDLAETIEMPANNLSVLFRKRQTIPT"
     gene            complement(1327..2412)
                     /locus_tag="VEM33_01585"
     CDS             complement(1327..2412)
                     /locus_tag="VEM33_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HYS50549.1"
                     /translation="MRRGLISWSRDEVPPSVLEGRLARLQERMRPAELGVVLIYTSFA
                     RPSAVAWLTHFVPYWNEALLAVFPTGAPVLLAAFSKRVHDWIRSVSHLGEVRSAPELG
                     RAAAAFLKERPPEPSRIGVLELDALPWPVAEPIMKSEYGRAVVDATGLFASIRQPADE
                     VEIRLARRAATIAVQAFKAISTGARRASELLSALERSARLDGAEEVLPRLAPDLRADA
                     TLRRLEGDVLLGERYAVELSVAYKATWVRITRCVSTQAAPPSWRGAADRFVEAVEHLN
                     EANLSLEPLAAGSARPGKNAAWTLETCRGGPPLSIVATGETPSPSALPGGSLACLSMQ
                     LALDDGPWLGGEPLILGSRGQPSRLLV"
     gene            complement(2417..>3489)
                     /locus_tag="VEM33_01590"
     CDS             complement(2417..>3489)
                     /locus_tag="VEM33_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HYS50550.1"
                     /translation="AAAMADEGIDILVVAGNPWRSDYLRFAADITPVEGHAFAFIERE
                     GPARVIVEHPAEAARLRSEQAELDVRWSLSAIEETETAIRRLGKRRIALAPRAAVPYR
                     LAMGPLVASIAPATALLDRLMLRKSPAEIEAFRSAAALADEGYAVFRGAARVGRPEYA
                     LVAEVEAFLRSRGCPENFQILGSGGREVRGMHPPTERRLAPGDLVTTELTPCVEGYYA
                     QICRTLVLGEPSATQQRAFAVYDEALEAGIAAVRPGANAGDVARAQNDVFRRQGLGAY
                     VTSDYTRVRGHGLGLYVDGRPAVLEDVDLDLEPDMTLVVHPNTYHPEVGYFVHGDSVR
                     VTPGGCEVLTRTPRRLFSVPLG"
BASE COUNT          657 a         1104 c         1110 g          618 t
ORIGIN      
        1 cgatcactac gcacgtttct tacgtaagcc aactaacgcg agatccttgc ccccagtgag
       61 cgcgttcgca aggtactgca cggtgacgta gagttcgtcc tcattgcacc aatagtcatt
      121 gataacgcca tatcgcgaat agacagagcg attgaagcca tcctcgaggt cgttgtacca
      181 gatcaccgtg cgaccgatta gcgcgacgac ccagaaccca ccgcccgcat ctccgtaggg
      241 gtgctgctcc cacttctcgg ggtcaattcg aattgactcc cagagtcgct gattctcctg
      301 gggcattcgc gcgtagccct gaccgattct cttgaggagg gccttcttcg aaataggttc
      361 ccagtcgatc atggcgccta gggagcagaa gcatagaagg catctactgt tggagttcca
      421 tccgctcgga ttgcaggcaa gcgctggtgt tccgtcatgt gggaattgtc tggcgctttc
      481 gaaacagcac gctaaggtta ttcgccggca tctcgattgt ttcggctaaa tcgaagcctt
      541 gctccttcgc caccgaagcg acctcttcca gatctcggag accccattcg gggttttggg
      601 cgcgcagggc actttcgaaa gccgcattgc tcggtgctgt gtgcctgccc gccatggagt
      661 aaggcccgta caggtacaag agaccggcct cgggaagggc gagccctgca ccggcgaaga
      721 gagccgtcgt caccgaccaa ggggctatgt ggatgagatt gaggcagacg atcgcatcga
      781 gcgtgggcac gggccaaggc aacgtgcgca cgtcgagatc aaggggttgg cgcacattgg
      841 caaggtttgc ggtcgccagc caagcggaga tcgatcgacg acagcccgcc tccatatcgg
      901 tgggttgcca gatcagccgc ttcagtgccc gagcgaaatg aaccacatgc tgtccggtgc
      961 cgctagcgac ctccagcacg aggccggttt tcggtagaac gcgttcgagg accttcagaa
     1021 tcggcccgct gtttcgctcc gctgaggaga ctaccagccg accatccgga agctcgtctg
     1081 aatgttcaca gcgaattgca gacctccatg agcgctaacg ttcaatcatc ttccgaaata
     1141 cgttctaatc tggcgcctca gtctacgctt gtcggaaatg ttcctccaaa tcaagactgt
     1201 ctgagtacga cataggattt cagctcttga ttggttgcgg gcggagggtt aggtatctga
     1261 attccctgga ggtgaaaccc cgctagagtc gattctgcat tgcagaatcg ttttttcaac
     1321 agcctgttag accaaaagac gactcggctg cccgcggctc ccgaggatga ggggctcgcc
     1381 gccaagccaa gggccgtcgt caagagctag ctgcatcgag aggcaggcaa gcgaaccgcc
     1441 cgggagcgcc gatggcgacg gtgtttcccc agttgccacg atggaaagcg gcgggccgcc
     1501 gcggcaagtc tcgagggtcc aagcggcgtt tttccctggt cgcgcgctcc ccgcggctag
     1561 gggttcgagg gaaagattcg cctcgtttag atgctcgacg gcttcgacaa agcgatccgc
     1621 ggcgccgcgc cacgacggcg gagccgcttg ggtggaaaca caccgagtga tgcgtaccca
     1681 ggtagccttg taggcgactg acaactcgac cgcgtagcgc tcgccgagga ggacgtcacc
     1741 ttcgagtctg cgcagtgtcg cgtccgcgcg aaggtccggc gctagccgag gcaacacttc
     1801 ttccgcaccg tctagccgcg ccgagcgttc cagcgcggaa agcagttccg acgcgcgccg
     1861 cgcccctgtg gaaatcgctt tgaaggcttg cacggcgatc gttgccgcgc gccgcgccag
     1921 ccgaatttcc acttcgtcag ccggctggcg gatcgaggcg aacaacccgg ttgcatcgac
     1981 gaccgctctg ccgtattccg atttcatgat cggttcagcc accggccagg gcagggcatc
     2041 gagttccagc acgccgatgc gcgagggctc tggaggacgt tccttcagga acgccgcagc
     2101 ggcgcggccc agttcgggtg cggatcgcac ttcgcccaag tgactgacgc ttcgaatcca
     2161 gtcgtgcacg cgcttggaga acgccgccaa gagcaccggc gctccggtcg ggaagaccgc
     2221 gagcagcgcc tcgttccagt acggcacgaa atgcgtgagc caggccacgg cgctgggacg
     2281 cgcaaaggac gtgtagatga gcacgacgcc cagttccgcg gggcgcattc gctcctgcag
     2341 gcgcgccaag cgcccctcga ggaccgaggg cgggacttcg tcgcgcgacc agctgatcaa
     2401 tccgcgccgc acggctctag cccaggggca ccgaaaacaa tcgccgtgga gtccgggtca
     2461 ggacctcgca gccaccgggt gtgacgcgca cggaatcgcc gtgcacgaaa tacccgacct
     2521 ccggatgata ggtgttcgga tggacgacga gggtcatgtc gggctcgaga tccagatcga
     2581 cgtcctcgag caccgcgggc cgcccgtcta cgtagaggcc caggccgtgg ccgcgcacgc
     2641 gggtgtaatc cgaggtcacg taggcgccca ggccctggcg gcgaaatacg tcgttttgcg
     2701 cccgcgcgac gtctcccgcg ttggctccgg ggcgaacggc cgcgataccg gcttcgagag
     2761 cctcgtcgta gacggcgaag gcccgttgtt gggtggcgct cggttccccg agaacgaggg
     2821 tgcggcagat ctgcgcgtag taaccctcca cgcacggggt cagctcggtc gtcactagat
     2881 cgccgggcgc gaggcgccgt tccgtcggcg gatgcattcc gcgcacttcc cggccgcctg
     2941 aaccgagaat ctgaaagttc tcgggacagc cgcgcgagcg caagaaagct tcgacttccg
     3001 cgacgagcgc atattcgggc cgcccgaccc gcgccgcgcc gcgaaaaacc gcgtagcctt
     3061 cgtcggcgag ggcggcggcg ctgcgaaacg cctcgatctc tgccggcgac ttccgcagca
     3121 tcaggcgatc gagcaaggcc gtcgccggtg cgatgcttgc gacaaggggt cccattgcaa
     3181 gacgataggg cacggcggcg cgcggggcga gcgcgatccg ccgcttgccg agccggcgga
     3241 tcgcggtctc ggtttcttcg atcgcagaga gcgaccatct cacatcgagt tccgcctgct
     3301 ccgagcgcag gcgcgcggcc tcggcagggt gctcgacgat gacccgcgcc ggcccctcgc
     3361 gctctatgaa cgcaaaagcg tggccttcga ccggggtgat gtcggccgcg aaacgcagat
     3421 agtcgcttcg ccagggattc ccggcgacca cgaggatgtc gatcccttca tccgccatcg
     3481 ctgcggcga
//
LOCUS       DASPZD010000085         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_923050, whole genome shotgun sequence.
ACCESSION   DASPZD010000085 DASPZD010000000
VERSION     DASPZD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923050"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /gene="pepN"
                     /locus_tag="VEM33_01595"
     CDS             complement(<1..1723)
                     /gene="pepN"
                     /locus_tag="VEM33_01595"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HYS50551.1"
                     /translation="MRDPAPVPIHLKDYAPPAFLIESVDLDVELLEDHARVRSRLALS
                     RNPKAADSNAPLVLDAEELELESVALDGRALAAGEYATDAAHLAVAKPPARFALETAC
                     KIYPRKNSMLMGLYAAENGFFTQCEAEGFRRITYFIDRPDVMARYSVTIHASREKYPV
                     LLSNGNLAASGDETNARHWVKWQDPYPKPSYLFALVAAKLDKFEDQFVTRSGRRVRLA
                     IYVDPGKLDQCGFAMQALKKSMKWDEDKFGLEVDLDNYSIVAVGDFNMGAMENKGLNI
                     FNTKYVLARPDTATDGDYLAIDRVIAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQ
                     EFGADMYSRAVERIREVRGLRSGQFAEDAGPMAHPVRPASYMEINNFYTSTVYEKGAE
                     VVRMIRTLIGTQNFRKGMDLYFARHDGQAVTCDDFVQAMADASGADLSQFMRWYDQAG
                     TPVLEIKGDYDAAARRYALFVKQSCPPTPGQETKLPFHIPLAVGLIGPDGRELLAEGT
                     RVLPVKQAEERFSFENVAAKPVPSLLRDFSAPVIVRHDYTEAELTHLMAHDSDAFNRW
                     EAGQRLAL"
     gene            complement(1784..>2282)
                     /locus_tag="VEM33_01600"
     CDS             complement(1784..>2282)
                     /locus_tag="VEM33_01600"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HYS50552.1"
                     /translation="LPKRNELADPAFSHHPKATLPLISAPGVELRVVAGSAFGKRSPA
                     PVLMQTLFVGVEIDAGRAFELEAGHEERAVYALEGDVTVSGTPLPRQHMAVLAHREAV
                     EVKALTRARLMLVGGEKMDGERLIWWNFVASSRELLEEAKLRWREQRFPRVPGDDEFI
                     PLPEA"
BASE COUNT          380 a          734 c          756 g          412 t
ORIGIN      
        1 cgagcgcaag ccgctggccg gcctcccagc ggttgaaagc gtcggaatcg tgcgccatga
       61 gatgcgtgag ctcggcctcg gtgtagtcgt gcctgacgat cacgggcgcc gagaaatcgc
      121 gcagcaacga cggcaccggc ttcgcggcaa cgttttcaaa cgagaaacgt tcttcagctt
      181 gtttcaccgg cagcacgcgc gtgccttcgg cgagcagctc gcgtccgtcg ggtccgatga
      241 gcccgaccgc gagcggaatg tggaagggca gtttcgtctc ctgtcccggc gtgggaggac
      301 aggattgctt cacgaacaac gcatagcgcc gtgccgctgc gtcgtagtcg cccttgatct
      361 cgaggacagg cgttcccgcc tggtcgtacc agcgcatgaa ctgcgagaga tcggcgcccg
      421 aggcatcggc catcgcctgc acgaaatcgt cgcaggtcac cgcctgcccg tcatgccgcg
      481 cgaagtacag atccatgcct ttgcggaaat tctgcgtgcc gatcagcgtg cggatcatgc
      541 gcacgacttc cgcgcccttc tcgtagacgg tactggtgta gaaattgttg atctccatgt
      601 aggaggccgg gcgcaccgga tgcgccatcg gccccgcgtc ctcggcaaac tgcccgctgc
      661 gcaggccgcg cacctcgcgg atgcgctcga ccgcccgcga atacatgtcc gcgccgaact
      721 cctggtcgcg gaacacggtg agcccttcct tgagcgacaa ctggaaccag tcgcggcagg
      781 tgacgcggtt gccggtccag ttgtgaaagt actcgtgcgc gatgacgcgg tcgatggcca
      841 ggtagtcgcc gtcggtggcg gtgtccggcc gcgcgagcac gtatttggtg ttgaagatgt
      901 tgaggccctt gttttccatc gcacccatgt tgaagtcgcc gaccgcgacg atcgagtaat
      961 tgtcgaggtc gacttcgagg ccgaacttgt cctcgtccca tttcatggat tttttcagcg
     1021 cctgcatggc aaaaccgcac tggtcgagct tgcccgggtc gacgtagatc gcgaggcgca
     1081 cgcggcggcc ggaacgcgtg acgaattggt cctcgaactt gtcgagcttc gccgccacca
     1141 gggcgaacag ataggagggc ttggggtagg ggtcctgcca cttcacccag tggcgggcat
     1201 tggtttcatc gccgcttgcg gcgagattgc cgttcgacag cagcacggga tacttttcgc
     1261 ggctggcgtg gatcgtcacg gaatacctcg ccatcacgtc gggccgatcg atgaagtacg
     1321 tgattcgccg gaagccttcc gcctcgcact gggtgaagaa cccgttctcc gccgcgtaca
     1381 gacccatgag catgctgttc ttgcgcgggt agattttgca cgcggtttca agcgcgaagc
     1441 gcgccggcgg cttcgcgacg gcgagatgcg cggcgtccgt cgcgtactcg ccggcggcga
     1501 gcgcgcggcc gtcgagcgcc accgactcga gctcgagctc ctcggcgtcg agcacgaggg
     1561 gtgcgttcga atcggctgcc ttcggattac gcgatagcgc gagacgcgag cgaacgcgcg
     1621 cgtgatcttc gagcaactcg acgtccagat cgacgctctc gatcagaaac gcgggcggag
     1681 cgtagtcctt gaggtgaatc ggaacgggtg cgggatcacg catcggcggc gggcgggttc
     1741 ggaggggatg ctcgtgcgcg cgagcgcacg ctctgcttcc ccctcatgcc tccggaagcg
     1801 ggatgaactc gtcgtccccc ggaacccgcg ggaagcgctg ctcgcgccag cgaagcttcg
     1861 cttcctccag gagctcgcgc gagctcgcga cgaagttcca ccagatcagg cgctcgccgt
     1921 ccatcttttc cccgccgacc agcatcaggc gcgcgcgcgt cagcgctttc acttcgacgg
     1981 cctcgcgatg cgcgaggacc gccatatgct gccgcggaag cggcgtcccg gaaacggtca
     2041 cgtccccttc gagcgcgtag acggcccgct cttcgtgccc ggcttcgagc tcgaatgcgc
     2101 gacccgcatc gatctcgacg ccgacgaaga gcgtctgcat gagcaccggc gcgggggagc
     2161 gcttgccgaa ggcgctgccg gcgacgaccc gcagctccac gcccggcgcg gaaatcagcg
     2221 gcagagtcgc cttggggtga tggctgaacg cgggatcggc gagctcgttc cttttcggta
     2281 gc
//
LOCUS       DASPZD010000086         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1787928, whole genome shotgun sequence.
ACCESSION   DASPZD010000086 DASPZD010000000
VERSION     DASPZD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787928"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..942
                     /locus_tag="VEM33_01605"
     CDS             208..942
                     /locus_tag="VEM33_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipo-like protein"
                     /protein_id="HYS50553.1"
                     /translation="MATYLAQPIRGAQTATASLEQLAAALKPGDVLLIEGNTRVSVAI
                     KYLTQSTWSHAMFHIGDALGPPAPGEAPKVLVEADMLYGIRGVPLSDVSGMNTRICRP
                     VGLSKEDLQAVIKFAVSHIGGRYDLKNVFDLARYLIPTPPVPVRWRRRMIAFGSGDPT
                     RAICSTLVAQAFQSVHYPILSEVKYVHDGTPGCNDCYHEVMHIRHHSLFTPRDFDISP
                     YFEVVKPTIVKDFDYRQIAWAQEDPV"
     gene            960..1679
                     /locus_tag="VEM33_01610"
     CDS             960..1679
                     /locus_tag="VEM33_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYS50554.1"
                     /translation="MPKYETTLTRSETVAEGTMAFHFAKPEGFKFTAGQSMNVSLIDP
                     PETDAKGNSRAFSIVSAPHENELVIATRMRDTAFKRVLKAMPAAGRVGLRGPAGLFTL
                     DPADARPAVFLAGGIGITPFVSMLREAAHSRLARDLWLFYSNRRPEDAAFLEELALPK
                     RHSRCHFVGTMVEMDKSSRPWSGETGFLDRAMLERHLKGLSANIYYVAGPPGLVEAMQ
                     KMLVAAGVAEEAIHTDEFFGY"
     gene            1696..>3777
                     /locus_tag="VEM33_01615"
     CDS             1696..>3777
                     /locus_tag="VEM33_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYS50555.1"
                     /translation="MLQRRSDPAIVIRGARQNNLKNLSLEIPTGELVVVTGVSGSGKS
                     SLVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDAIEGIPPAIAIDQTNPVRTSRS
                     TVGTMTELNDHLKLLFARAGQLYCRGCGRPVKRDTPGSVYEEVVERAKAAGDPRIVVT
                     FPVTVPKNFKEEEVQALLKAQGYDRIHSRSGALLEVVQDRVKMSSAEKARVIEGIEAA
                     MKIGQGKVNLHPLDEEGKAGTPWRFSSDLHCADCDIHYSEPMPALFSFNSPLGACETC
                     RGFGRVIGIDYGLIVPDESKTLREGAIRPWQSPSFHECQDDIEKYARKRGVPLDVPFR
                     DLSAKERAWVLEGEPEWVSWRKSWPGVWYGVARFFRWLETKAYKMHVRVLLSRYRAYT
                     PCSACNGARLKPEALLWRVGTKEDADRVLDPARRFMPQGAKFSDDVLRELPGLTVHDL
                     VLLPAERARAFFEHLHLPAPLDEATDLLLTEIRARLRYVCEVGLGYLTLDRQSRTLSG
                     GEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMGRVIGVMHRLRDAGNSLVVVEHD
                     PQIMLQADRILDMGPGPGERGGEIVFFGTPDELKQSKTLTAEYLSGRKRADSGKASAT
                     PDGKARKIELLGAAEHNLKDIDISIPLERLVCITGVSGSGKSTLVQDVLYAALRKAKG
                     KPTELPGAFREL"
BASE COUNT          683 a         1286 c         1190 g          618 t
ORIGIN      
        1 aaaccatgca ttggatgaag accgtgattc gcgcgttccc gccgggagta aacacgcaaa
       61 aggaagaaag tgcttcccgg aactccgtaa taaaatcagg ctctcggaaa atcgggaacg
      121 ccgcggatcg aagcgtcgtc gaatcgctac agaaagagac ccgcgcagct actcatgaac
      181 cccgtactcc gatggatggg cggacgcttg gccacctacc tggcacagcc gatccgcggc
      241 gcccaaaccg cgaccgcctc gctcgagcag ctcgcggccg cgctcaagcc gggcgatgtc
      301 ctgctcatcg aggggaacac gcgcgtcagc gtcgccatca aatacctgac ccaatcgacc
      361 tggtcgcacg cgatgttcca tatcggcgat gcgctagggc cccctgctcc cggcgaagcg
      421 cctaaggtcc tggtcgaagc cgacatgctc tacgggattc gcggcgtacc gctgtccgac
      481 gtctccggca tgaacacccg catctgccgg ccggtgggcc tctcgaagga ggatctgcag
      541 gcggtgatca agttcgcggt atcccacatc ggcggccgct acgacctgaa gaacgttttc
      601 gatctcgcgc gctatctgat acccacgccg cccgtcccgg tgcgctggcg ccgccgcatg
      661 atcgccttcg gcagcggcga tccgacgcgc gccatctgct cgacgctggt cgcccaagcg
      721 ttccagtcgg tgcattaccc gatcctgtcc gaggtcaagt acgtccacga cgggacgcct
      781 gggtgcaacg actgctatca cgaggtcatg cacatccggc accacagctt gttcacgccg
      841 cgcgacttcg acatctcgcc gtacttcgag gtggtgaagc ccaccatcgt caaggacttc
      901 gactatcgcc aaatcgcctg ggcccaggaa gatccggtct gaccggagaa agagctgcga
      961 tgcccaaata cgaaaccacg ctcacccgca gcgagaccgt cgccgagggc acgatggcgt
     1021 tccatttcgc caagcccgag gggttcaagt tcacggccgg acaatcgatg aacgtgtccc
     1081 tgatcgatcc gcccgagacc gacgccaagg ggaactcccg cgccttctcg atcgtcagcg
     1141 ccccccacga gaacgagctc gtcatcgcga cgcgcatgcg cgacacggcg ttcaagcggg
     1201 tgctcaaggc gatgcccgct gccggccgcg tcggcctgcg cggtccggcg ggccttttca
     1261 cgctggatcc cgcggacgcc aggccggcgg tattcctcgc cggaggcatc ggcatcacgc
     1321 ccttcgtgag catgttgcgc gaggcggcgc attcgcggct cgcgcgggac ctgtggctgt
     1381 tctattcgaa ccgccggccc gaggacgcgg cctttctcga ggagctggcg ctccccaagc
     1441 gccattcccg ctgccatttc gtcggcacca tggtcgaaat ggacaagtcg agccgtccct
     1501 ggagcggcga gaccggcttt ctcgaccgcg cgatgctcga gcgccacctg aagggcctct
     1561 ccgcgaacat atattacgtg gcagggccgc ccgggctggt cgaggccatg cagaaaatgc
     1621 tcgtcgccgc gggcgttgca gaagaggcga tccacacgga cgagttcttc ggctactagg
     1681 cgataaaata gcggcatgct gcaacgccgc tccgaccccg cgatcgtcat ccgcggcgcc
     1741 agacagaaca acctcaagaa cctctcgctc gagatcccca cgggggagct cgtcgtcgtg
     1801 accggcgtct cggggtcggg aaaatcctcg ctcgttttcg acaccctcta cgccgagggg
     1861 cagcggcgct acgtcgagac gttcagcccc tacgcgcgcc agttcctcga ccgcatggac
     1921 aagccccagg tcgacgcgat cgaggggatc ccgcccgcga tcgccatcga ccagaccaac
     1981 ccggtgcgca cctcgcgctc gacggtcgga acgatgaccg agctgaacga ccacctgaag
     2041 ctcctcttcg cgcgcgcagg tcagctctat tgccgcggct gcggccggcc cgtgaagcgc
     2101 gacacgcccg gctctgtata cgaggaggtc gtggagcgcg ccaaggccgc gggcgacccg
     2161 cgcatcgtcg tcaccttccc ggtgacggtg ccgaagaact tcaaggagga ggaagtccag
     2221 gctctgctca aggcccaggg gtatgaccgc atccattcgc gctcgggcgc gctcctggaa
     2281 gtcgtgcagg accgcgtgaa gatgtcctcg gccgagaagg cgcgcgtgat cgagggcatc
     2341 gaagccgcga tgaagatcgg ccagggcaag gtcaacctcc atccgctgga cgaggaaggc
     2401 aaggcgggaa ccccctggcg cttctcctcg gacctgcact gcgcggactg cgacatccac
     2461 tattcggagc cgatgcccgc cctgttctcg ttcaactcgc cgctcggcgc ctgcgagacc
     2521 tgccgcggct tcgggcgcgt gatcggcatc gactacggcc tcatcgtccc ggacgagtcg
     2581 aagacgctgc gcgaaggcgc gatccggccc tggcaatctc ccagctttca cgagtgccag
     2641 gacgatatcg agaagtacgc caggaaacgc ggcgtgccgc tcgacgttcc ttttcgcgat
     2701 ctctccgcga aggagcgcgc atgggtgctc gagggcgagc ccgagtgggt gagctggaga
     2761 aaatcctggc cgggcgtctg gtacggcgtc gcgcgcttct tcaggtggct ggagaccaag
     2821 gcctacaaga tgcacgtgcg cgtgctcttg tcgcgctacc gcgcctacac gccttgttcg
     2881 gcctgcaacg gcgcgcgcct gaagcccgag gcgctcttgt ggcgtgtggg caccaaggag
     2941 gatgccgacc gcgtgctcga tcccgcgcgg cgcttcatgc cgcagggcgc gaaattctcg
     3001 gacgacgtgc tgcgcgagct tcccggcctt acggtgcatg acctggtgct gttgcccgcg
     3061 gagcgtgcgc gcgcgttctt cgagcacctg cacttgcctg cgccgctcga cgaagccacc
     3121 gacctccttc tcaccgaaat ccgcgcccgg ctgcgctacg tctgcgaagt cggcctcggc
     3181 tatctcacgc tcgatcgcca gtcgcgcacg ctctcgggcg gcgaggtgca gcgcatcaac
     3241 ctgaccaccg cgctcggcac ttcgctcgtc aataccctgt tcgtgctgga cgaaccgtcc
     3301 atcggactgc acccgcgcga catgggccgc gtgatcggcg taatgcaccg gctgagggat
     3361 gccgggaatt ccctcgtcgt ggtggagcac gacccgcaga tcatgctgca ggccgaccgc
     3421 atcctcgaca tgggccctgg acccggtgaa cgtggcggcg agatcgtgtt cttcggcacg
     3481 cccgacgagc tgaagcagtc gaaaacgctc actgcggaat acctttcggg caggaagcgc
     3541 gccgacagcg gcaaggcatc cgcgacgccg gacgggaaag cgcggaagat cgagctgctc
     3601 ggcgccgccg agcacaatct gaaggacatc gatatcagca ttccgctcga gcggctggtg
     3661 tgcatcaccg gcgtttcggg ctcggggaaa tccacgctgg tgcaggatgt gctctacgcc
     3721 gcgcttcgaa aggcaaaggg caagccgacc gaactgccgg gtgcgttccg cgagttg
//
LOCUS       DASPZD010000087         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_79775, whole genome shotgun sequence.
ACCESSION   DASPZD010000087 DASPZD010000000
VERSION     DASPZD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79775"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            690..2045
                     /locus_tag="VEM33_01620"
     CDS             690..2045
                     /locus_tag="VEM33_01620"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50556.1"
                     /translation="MSDSTATRQQQQRLSAAQAGLRLGERLRQLRAAAGMTQTDLAGD
                     RFSKEYVSQIERGKTKPTRDTIEWLAGRLGVDAGFLANGVSADERGRVDAALARAEAL
                     LEARRNDEALAEFDHIRAAVLATGLPELEARALSGEATVRMRAGEVREAIALLERARA
                     LTESSAFSHVERADVLFRLGVARFKLNSVQSAIALFDEALKVAEQSEIPSDQLRSNIL
                     AWRSRCYRRRRDLEAAREDVERALELAESLNDTRTAADVYFQASIIADREGHWVLARS
                     YAERAKAAYEELSDRGNLGRLLNNLGGINFLLGHPEDALAFLKDAVRIALEVGNDAEA
                     AHAVNGVAQVHLRGGDIARAEEQARYALKLLGDRVDEVHEIGNAQLVLGRALLEQDRL
                     DEAEEAFKAGERAYDQMSSGSHRAEAWIAQGDLAARRGDDRTAAKLYRQAADALQDVR
                     F"
BASE COUNT          378 a          690 c          710 g          341 t
ORIGIN      
        1 agccctccac gtcggcgcag cgattctcct ggccggcgcc gccgtagcgg tcctgacgat
       61 ccgcaaagtc caacacgcag aagagcccca ggcaatccct gaacaagcag tcggcgcgta
      121 gctcacgcca ccacagacca aaggccggag gctaagccag gcgaagccgg cttagccgca
      181 ggcgaactga tccgtgccgc gatgccgaag gcaccgcgac acggacggtt gacgacattc
      241 gcgggcgatc cccggtagag cgcgcgggct cagcccgcgc gcgttcagcg cgaagcgccg
      301 cacaaacccg cgggccgaaa gcaaagcttt cggcccgaca aagctttcgg cccgagaaag
      361 aggaagaccc ccggctgcgg ctccgggggt cgattccttt caggcacttc gctcttttgg
      421 gcttgatccc cgggacttgg ctctgcggcc cagccacgcc ccgtttggat cgcaacctgg
      481 tgaagcgcct cctgcagccg accctggcgt ctggttcggt tacaagatga cctcactgta
      541 cgagccttcc ggtgccctgt caagtagctc agtcaagtga ttttgagtag gcttacttga
      601 ctggttttat gcccagacat gacctttctg ggggatgctt gaaaggacag gttgagattt
      661 ggtgaagtat gtcgtctact acgccgtacg tgtctgattc gactgccacc cgccagcagc
      721 agcagcgact gtccgccgct caagccgggc ttcgcctcgg cgagcggctt cgacagctgc
      781 gggccgcggc ggggatgacc cagacggatc tggcgggtga tcgcttctcg aaggagtacg
      841 tgagccagat cgagcgcggc aagacgaagc cgacgcggga cacgatcgaa tggctcgcgg
      901 ggcgccttgg cgtcgatgcg ggtttcctcg ccaacggggt ctccgccgac gagcgcgggc
      961 gggtcgacgc ggcgctcgcg cgcgccgagg cgctgctcga agcacggcgg aacgacgagg
     1021 cgctggcgga gttcgaccac atccgagcgg cagtcctcgc gaccggcttg cccgagctcg
     1081 aagcgcgggc actttcgggc gaagcgaccg tccgcatgag ggccggcgag gtgcgcgagg
     1141 cgattgcgct gctcgagcgc gcgcgggcac tgaccgaaag cagcgcgttc tctcacgtcg
     1201 agcgcgcgga cgtcctcttc cgcctcggcg tcgcgcggtt caagctcaac agcgtccagt
     1261 ccgcgatcgc gctgttcgac gaggcgctca aggtcgcgga gcagtccgag attccctccg
     1321 atcagctccg ctcgaacatt cttgcgtggc gttcgcggtg ctaccggcgt cgccgcgacc
     1381 tcgaggccgc gcgtgaggac gtcgagcgcg cactcgagct cgcggagagc ctgaacgaca
     1441 cgcgcactgc cgcggacgtc tacttccagg cgtcgatcat cgccgatcgc gaaggccact
     1501 gggtgctcgc gcggtcgtac gcggagcgcg ccaaggctgc atacgaggag ctgtccgacc
     1561 gcggcaatct cggccggctg ctgaacaacc tcggtggcat caacttcctg ctcggacatc
     1621 cagaagacgc actcgcgttc ctgaaggacg cagttcgcat cgccctggag gtcggcaacg
     1681 atgctgaggc ggcgcacgca gtcaacggcg tcgcccaggt tcacctccgt ggtggggaca
     1741 tcgcgcgcgc cgaggaacag gcccgctatg ccctcaagct gctgggcgac cgtgtcgacg
     1801 aggtgcatga gatcggcaac gcgcagctcg tgctcgggcg cgcgctactc gagcaggacc
     1861 gtctcgacga agccgaagag gcgttcaagg caggagagcg ggcgtatgac cagatgtcgt
     1921 cagggagtca ccgcgctgag gcgtggatcg cacagggcga tctcgctgcc cggcgaggag
     1981 acgaccggac ggcggcaaag ttgtaccgcc aggctgccga tgccctgcaa gacgtccgct
     2041 tctaaccgag aaaggaggtg agcctgtgtc gaaagcccgt ctcatctatt tcgcagtctt
     2101 cgcgatcctg atcgctatg
//
LOCUS       DASPZD010000088         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_681712, whole genome shotgun sequence.
ACCESSION   DASPZD010000088 DASPZD010000000
VERSION     DASPZD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681712"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VEM33_01625"
     CDS             complement(<1..1341)
                     /locus_tag="VEM33_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HYS50557.1"
                     /translation="MRRIVLIAPFIAALAACALKSPPDPGETRAQALPNMTLPPQWAA
                     QSGSAGAVANGWLATFNDPQLEALVREALVYNLDLQLAAARVEQATGYARLAGATLYP
                     QVNLLARGGGKLSGDSSGLQGVGVFANWELDLWGRVRAGREAARLQYVSAELEAEYAR
                     QSIAALVAKSWFLATEARLQKGVAEDMVRASQQLVNLARERQRIGSGNEYDTVVAQAG
                     LQTQLDVVQQFDLAYRQSVRALEALAGRYPAAALDVPARLPAKPGPVPVGMPSELLER
                     RLDVVTAERRVAAALYRTEEAKAARLPKISLTAAVSSISSDLFVLKDRDNPVWSAGAS
                     LLAPIYLGGALQSQVEIRTAEQKQALAEFGRVGARAFGEVEDALSSGFTLDERATILA
                     RAVAENERALALANIRYRVGSGDLRAVQQQQLALSSSRTALLRVTSEQLTQRVNL"
     gene            complement(1350..2306)
                     /locus_tag="VEM33_01630"
     CDS             complement(1350..2306)
                     /locus_tag="VEM33_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYS50558.1"
                     /translation="MEAILLAIYSFFVWLIFIKLKWLPWNTRSQVIVVIIPVVGMVAL
                     ILLLNTFAPSSADVRVVNYVVQINPQVRGMVIEVPAEGNRPMKKGEVLFRIDPTPYRL
                     QVDEIQAQLTLAQTRLRQSRELAGTGAGNRFDVERYETESAQYQAKLANARWELDQTT
                     VYTPADGTPINVQLRRGSMAVPFPVAPAMTFVENEHQIIALFHQNELHQVAPGDEAEV
                     ALKTLPGKVLKAKVNSIVWAQGQGQLTVSGVLPQTAAAPQAPGRFAVKLDLEEKDLAT
                     FLAAGAVGDAAIYTQHLEAIHIIRKVVLRVGSYLNYLIPKLH"
     gene            complement(2306..2746)
                     /locus_tag="VEM33_01635"
     CDS             complement(2306..2746)
                     /locus_tag="VEM33_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HYS50559.1"
                     /translation="MADVLAIVVLCIVPIAAIVLFWLVHILPEKIAEKRHHPQKDSIK
                     TLCLLSLVFGGMLWPIAWLWAYTKPVGYRLAYGTDKHDDYFHEMGHQAKAGTLPRAEI
                     VHLKEELDHLAEGRKLPQELKALRDDLEAVLSGAASGDAERSAS"
BASE COUNT          486 a          991 c          969 g          512 t
ORIGIN      
        1 caggttgacg cgctgagtca gctgctcgct cgtcacgcgc agcaacgcag tgcgcgagga
       61 actaagggcg agctgctgct gctgcaccgc tcgcaggtct cccgatccca cgcggtaacg
      121 tatgttggcg agcgctagcg cgcgctcgtt ttccgcgacg gcgcgggcga gtatcgtcgc
      181 gcgctcgtcg agcgtgaagc cggaggagag cgcgtcctcg acctcgccga acgcccgcgc
      241 tcccacgcgg ccgaattcgg ccaaggcctg cttctgttcc gcggtccgga tttccacctg
      301 cgattgcagc gcgcctccga gatagatcgg cgcgagcagc gacgctccgg cgctccacac
      361 ggggttgtcc cggtccttga gcacgaagag gtcgctcgat atgctcgaca cggcagcggt
      421 cagggagatt ttcggcaacc gcgccgcctt cgcctcctcg gtgcggtata aggccgcagc
      481 cacgcggcgt tcggccgtca ccacatcgag ccgacgctcc agcagttccg agggcattcc
      541 taccggcacc ggccccggct tcgccgggag ccgcgcgggc acgtccaggg ccgcagcggg
      601 atagcgcccc gccagcgctt cgagggcccg gaccgattgc cgataggcaa gatcgaattg
      661 ctgcacgaca tcgagttggg tttgcaggcc cgcctgtgcg accacggtgt cgtattcatt
      721 gccgctgccg atccgctgcc gctcccgcgc caggttcacc agttgctgcg aggcgcgcac
      781 catgtcctcc gcaacgccct tctgcagccg cgcctccgtc gcgagaaacc agctcttggc
      841 gacgagcgca gcgatcgatt gacgggcgta ctccgcctca agctccgcgg agacatactg
      901 gagccgcgcc gcctcgcggc ccgcgcgcac ccggccccac aggtcgagct cccagttcgc
      961 gaagacgccg acaccttgaa gaccgctcga atctccgctc agcttgccgc cgccgcgcgc
     1021 gagcaggttc acctgcgggt agagcgtcgc gcccgcgagc ctcgcgtagc ccgtggcctg
     1081 ctcgacgcgc gcagcggcga gctgcaagtc gaggttgtac acaagagcct cgcgcaccag
     1141 cgcttcgagt tgcggatcgt tgaatgtcgc gagccatccg tttgcaacgg ccccggctga
     1201 gccgctctgc gccgcccact gcggcggaag cgtcatgttc ggaagtgcct gcgcgcgcgt
     1261 ttcgcccggg tcgggcggcg acttcagggc gcaagcggca agcgcggcga tgaacggagc
     1321 gatgagaacg atcctgcgca tgcgccgcct caatgcagct tcggtatgag gtagttcagg
     1381 tacgagccta cccgcagcac aaccttgcgg atgatgtgga tcgcctcgag atgctgcgtg
     1441 tagatcgccg cgtctcccac tgcgcccgca gcgagaaacg tggcgagatc tttttcctcg
     1501 aggtcgagct tgaccgcaaa gcggcccggg gcctgcggtg cggccgcggt ctgcggcaag
     1561 acgccgctca cggtcagctg tccctgaccc tgggcccaca cgatcgagtt gaccttggcc
     1621 ttgagcacct ttccgggcag ggttttcaac gccacttcgg cctcgtcgcc gggcgcgacc
     1681 tggtgcagct cgttctggtg aaacagcgca atgatctgat gttcgttctc cacgaacgtc
     1741 atcgcgggcg caaccgggaa cggcacggcc atcgaaccgc gccgcaactg cacgttgatc
     1801 ggcgttccgt cggccggcgt atatacggtg gtctggtcga gctcccagcg cgcgttggcg
     1861 agtttcgcct ggtactgcgc cgactcggtt tcgtagcgct caacgtcgaa gcggttgccg
     1921 gcgccggtcc ccgccaactc gcgcgattgc ctgaggcggg tctgcgcgag cgtgagctgc
     1981 gcctggattt cgtcgacttg cagccggtag ggcgtcgggt cgatgcgaaa cagcacctca
     2041 cccttcttca tcgggcggtt accctcggcc ggaacctcga tcaccatgcc gcgcacctgc
     2101 gggttgatct gcaccacgta gttgacgacg cgcacgtccg cggacgacgg tgcaaaggtg
     2161 ttgagcagaa ggatgagtgc gaccatcccg acgaccggga tgatgacgac gatgacctgg
     2221 ctcctggtgt tccacggcag ccacttgagc ttgatgaata tcagccagac gaagaacgag
     2281 tagatcgcaa gtaatatcgc ttccattacg aggcgctcct ctcggcgtcg cccgaggcgg
     2341 cgccggacag gaccgcctcg aggtcgtcgc gcagcgcctt caactcttgc ggaagcttgc
     2401 ggccctccgc gaggtggtcg agctcctcct tgaggtgcac gatttccgcg cgcgggagcg
     2461 tgcccgcctt cgcctggtgg cccatctcgt ggaagtagtc gtcgtgcttg tccgtaccgt
     2521 aggcgaggcg gtagccgacc ggcttggtgt aggcccagag ccaggcgatc ggccagagca
     2581 tcccgccgaa gacgagcgag agcaggcaca gcgtcttgat cgaatccttc tgcggatgat
     2641 ggcgcttctc ggcgatcttc tcgggcagga tgtgaacgag ccagaaaagc acgatcgccg
     2701 cgatcggaac gatgcagagg acgacgatgg ccaggacgtc cgccatcgta tcgagcgcgt
     2761 cgcccgacag gaagctcgcc cacgcgccgg tgggcgccga cgcaagtccg gcaagggacc
     2821 cggcgaaggc ccatgcgcga aaaggtcggc cgcacggtga tcctgatccg tagccttgca
     2881 tcttcgggct cccctctagg tttccggagg aaagaattgt gcccggttag ggttgtaacc
     2941 gcagcctctt ctgcatgg
//
LOCUS       DASPZD010000089         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1052993, whole genome shotgun sequence.
ACCESSION   DASPZD010000089 DASPZD010000000
VERSION     DASPZD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052993"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VEM33_01640"
     CDS             complement(<1..624)
                     /locus_tag="VEM33_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HYS50560.1"
                     /translation="MNGQRRNRGLSPVLRGTLADFGGVYLVNGLVAFIFAASGPVAII
                     LAVGTRGGLAPSDLSSWIFGAFFVNGLISIAFCWLYRQPLVFFWTIPGTVLVGPALAH
                     LSYAEVIGAFIATGILMLALGLSGWVRRAMEAVPMPIVMGMVAGVFLRFGLDLVLAVR
                     DDFWIAAPMVAAFVAIGASQRLARLVPPLIGALAVGVIVIAVVGRFEV"
     gene            complement(669..2048)
                     /gene="purB"
                     /locus_tag="VEM33_01645"
     CDS             complement(669..2048)
                     /gene="purB"
                     /locus_tag="VEM33_01645"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYS50561.1"
                     /translation="MPPHSPPSPLNALSPLDGRYHKQLEPLRTCFSEAALFRYRIAVE
                     IRWLLALAAESAIGEIKPFSKATHAELERIIETFSEEDAAQVKAVEAETNHDVKAIEY
                     WLKKRLAGNPEGARVAEFVHFACTSEDINNLAYGLMLKEGRDKVLLPALDRVIEALAA
                     LARRLADAAMLARTHGQPASPTTLGKELANVVHRLRRARRSIASVSLTGKMNGAVGNY
                     NAHVAAYPSFDWEGFARAFVESLGLEFNPYTIQIEPHDSLAEQFDAVARANTVLLDLD
                     RDLWGYVSLGYFRQRTQSGEVGSSTMPHKVNPIDFENSEGNLGIANALLRHLSEKLPV
                     SRWQRDLSDSTVLRNIGVALGHSLLAYESCLKGLGKLEADPARMLEDLEANWEVLGEA
                     VQTVMRRYGLPDPYEQLKALTRGKRVDGEAMRAFIRGLKLPEAEKARLLKLTPAGYLG
                     KAAELARRL"
     gene            2062..2682
                     /locus_tag="VEM33_01650"
     CDS             2062..2682
                     /locus_tag="VEM33_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HYS50562.1"
                     /translation="MANSTDFITRVKPAQFERLLAEVRACRICEAHLPNPPRPVLRAS
                     PTACVLIVGQAPGRRVHESGIPWNDPSGDLLRKWLRIGREDFYDERRIAIVPAGLCYP
                     GTGDSGDLPPRPECAPHWHPRLRALLPRIRLTLLIGAYAQAYYIGKRRKKTLAETVRA
                     RDEYLPEFFPLPHPSPRNRLWMKKNAWFEREVLPQLRRRLKAVRMI"
BASE COUNT          477 a          915 c          894 g          432 t
ORIGIN      
        1 gacctcgaac ctgccgacca cggctatcac gatcacgccg acggccagag cgccgatgag
       61 cggcggcacg agccgcgcga gccgctgcga ggcgccgatc gcgacgaatg cggccaccat
      121 cggcgccgcg atccagaagt cgtcgcggac ggcgagcacg agatcgaggc cgaaacgcag
      181 gaacacgccc gccaccatgc ccatcacgat cggcatcggc accgcctcca tcgcccgccg
      241 cacccatccg gagagtccca gcgcgagcat gaggattccg gtcgcaatga aggcgccgat
      301 cacttcagcg tagctcaggt gggccagcgc gggcccgacg agcaccgtcc ccggaatcgt
      361 ccagaagaaa acgagcggct gccgatacag ccagcagaag gcgatgctga tcagcccgtt
      421 gacgaaaaac gcgccgaata tccacgagga caggtccgac ggcgcaagcc ccccgcgcgt
      481 gcccacggcg aggatgatag ccaccggacc cgaggccgcg aagatgaacg cgaccaggcc
      541 gtttacgagg tagacgccgc cgaaatcggc gagcgtcccg cgcaaaaccg gggacagacc
      601 acggtttcgc cgttgtccgt tcatctttcg atgccagctg gctggaaaac cgtggtctgt
      661 ccccggtttt agagcctgcg ggcgagctcg gctgccttgc cgaggtagcc ggcaggcgtg
      721 agtttgagca gccgcgcctt ctcggcctcc ggcaacttga gcccgcggat aaaggcgcgc
      781 atcgcttcgc cgtccacgcg cttgccgcgc gtcagcgcct tgagctgctc gtagggatcg
      841 gggaggccgt agcggcgcat cacggtctgc accgcctcgc cgagcacttc ccagttggcc
      901 tccaggtcct cgagcatgcg cgcgggatcc gcttcgagct tgcccaggcc cttcaagcag
      961 gactcgtacg cgagcaggct gtggccgagc gcgacgccga tgttgcgcag caccgtcgaa
     1021 tccgacaggt cgcgctgcca gcgcgatacg ggcagcttct cggaaagatg ccgcagcagc
     1081 gcgtttgcga tcccgaggtt gccctctgaa ttctcgaaat cgatgggatt gaccttgtgc
     1141 ggcatggtcg aggagccgac ctcgcccgac tgcgtccttt gcctgaaata gcccagggag
     1201 acgtagcccc acagatcgcg gtcgagatcg agcagcacgg tgttggcgcg cgcgaccgcg
     1261 tcgaactgct cggcgaggct gtcgtgcggc tcgatctgga tggtgtacgg attgaattcg
     1321 aggccgagcg actcgacgaa cgccctcgcg aagccctccc agtcgaagct cgggtacgcg
     1381 gcgacgtggg cgttgtagtt gcccaccgcg ccgttcatct tcccggtgag ggacaccgag
     1441 gcgatcgagc ggcgcgcgcg ccgcagtcgg tgcaccacgt tcgcaagctc cttgccgagc
     1501 gtcgtcggag aggcgggctg cccgtgggtg cgcgcgagca tcgcggcgtc ggcgagccgc
     1561 cgggcgagcg ccgcgagcgc ctcgatcacg cgatcgagcg cgggcagcag aaccttgtcc
     1621 cgcccttcct tcagcatcag tccgtaggcg agattgttga tgtcctcgga ggtgcaggcg
     1681 aaatgaacga actcggcgac gcgtgcgcct tccggattgc cggccaggcg cttcttcagc
     1741 cagtactcga tcgccttgac gtcgtggttg gtctccgcct cgacggcctt tacctgcgct
     1801 gcgtcctcct cggagaaggt ttcgatgatc cgctcgagct cggcgtgagt cgctttcgaa
     1861 aaaggcttga tttcgccgat cgcggactcg gcggctagcg ccaggagcca gcggatctcg
     1921 actgcgatgc gatagcggaa gagcgcggct tcgctgaagc aggtgcgcag cggctcgagt
     1981 tgcttgtgat agcgtccgtc cagaggggat aatgcattga gcggtgacgg cggcgaatgc
     2041 ggcggcatca acggagcccg tatggcgaac agcaccgatt ttatcacgcg ggtgaagccc
     2101 gcccaattcg agcgcctgct cgccgaagtg cgcgcgtgca gaatctgcga ggcccatctt
     2161 cccaacccgc cgaggccggt gctgcgcgct tcgcccacgg cgtgcgtcct catcgtcggg
     2221 caggcgcccg gccggcgcgt gcacgagagc ggcattccct ggaacgaccc ttcgggcgat
     2281 ctgctgcgca aatggctgcg catcgggcgc gaggatttct acgacgagcg ccgcatcgcc
     2341 atcgtcccgg cgggcctgtg ctacccggga actggcgatt cgggcgacct cccgccgcgc
     2401 cccgagtgcg ccccgcactg gcacccgagg ctgcgcgcgc tgctgccgcg catccggctg
     2461 acgctcctca tcggcgccta tgctcaggcc tattacatcg gcaagcgcag aaagaagact
     2521 ctggccgaga ccgtgcgtgc ccgcgacgaa tacctgcccg agtttttccc gctgcctcac
     2581 ccgagcccgc gcaaccggct ctggatgaag aagaacgcct ggttcgagcg cgaagtgctg
     2641 ccgcaactgc ggcgaaggct caaagccgtc cgtatgatat aagctcatcc gaaatgaagc
     2701 tcatcggctc cctcgcca
//
LOCUS       DASPZD010000090         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1186159, whole genome shotgun sequence.
ACCESSION   DASPZD010000090 DASPZD010000000
VERSION     DASPZD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186159"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VEM33_01655"
     CDS             <1..331
                     /locus_tag="VEM33_01655"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50563.1"
                     /translation="VRTYSSALMNYRDQVLEFKPLRAQPGDAQVTVRSEVRQSGTQAV
                     SIDYEMEKTPSGWKVYDVKISGASLAATYRDTFTEEVRNHGIEGLIDSLSSKNRQIDA
                     RRASINT"
     gene            372..1580
                     /locus_tag="VEM33_01660"
     CDS             372..1580
                     /locus_tag="VEM33_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYS50564.1"
                     /translation="MKIRDSYLTAAAALSLAAVAALLSGCEPANSQPHTGMRPPEVAV
                     VTVQPQSVPATFEYIGQTAGSREVEVRARVTGIVLKRNYREGAPVAQGQSMFTIDPAP
                     FEVAAARAEAALASAEAKLAQAKRNSARLKPLFEIKAASQKDFDDAASAEQVAEAEVK
                     TARANQADARLNLGYTRVEAPIAGIASRAQRSEGNYVSGPEVLLTTVSQIDPIYVLFS
                     VSDEERVRLAREVDSGRLVLPRDRRFEVTVKLADGSVYSESGKPNFTDVRVSGSTSTS
                     EARAELPNPAGFLHPGQFVRVTLKGAERPATILVPQRAVLEGPKGKFVYVVNAESKAE
                     SRPVELGDWHGDAWIVTAGLNAGDRVIVDGVMKIGPGAPVAVAELQPRETNPAKNGTA
                     EGRASVVAQK"
     gene            1593..>2323
                     /locus_tag="VEM33_01665"
     CDS             1593..>2323
                     /locus_tag="VEM33_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYS50565.1"
                     /translation="MFSRFFIDRPIFAAVLSIFIVIAGLAAMRNLPIAQYPEIAPPVV
                     TITANYPGASAGVIEQTVAAPLENQINGVEHMIYMSSTSTSNGQVQIHVTFDIGTDID
                     RATVNINNRVRQVEPRLPADVRRLGVTVEKSSSSFLLVAAFYSPDGRYDDLYISNHVT
                     MNVLDVVKRIPGTTNVQIFGAKDYAMRIWVKPDRLTQLKLTTADLIAALNEQNAQFAA
                     GKVGQTPSGGPQELVYTITTKGRLA"
BASE COUNT          457 a          777 c          741 g          348 t
ORIGIN      
        1 cgtgcgcacg tattccagcg cgctcatgaa ctaccgcgac caggtgctcg agttcaagcc
       61 cttgcgcgcc cagcctggcg acgcgcaagt cactgtcagg tccgaagtca ggcagtcggg
      121 cacgcaagcg gtttccatcg actatgagat ggagaagacg ccgtccggat ggaaggtgta
      181 cgacgtcaag atcagcggcg cgagcctcgc cgcgacttac cgggacactt tcaccgaaga
      241 agtgcgcaac cacggcatcg agggcctgat cgattctctc tcaagcaaga atcgccaaat
      301 cgacgcgcgg cgcgcgtcga tcaacaccta acgcggactc acacccgctc caactgaaag
      361 gaagcaagat catgaaaatc agggactcct atttgaccgc cgctgcggca ctgtctttgg
      421 ccgcggttgc ggcgctgcta tcgggctgcg agcccgccaa ttcgcaaccg catacaggca
      481 tgcggccgcc ggaagtcgcg gtggtaacag tgcagccgca atcggtgccg gcgacatttg
      541 aatacatcgg ccagaccgcg ggctcgcgcg aggtcgaggt gcgcgcgcgc gtgacgggca
      601 tcgtcctgaa acgcaactac cgcgaaggcg cgccggtcgc gcaggggcag tcgatgttca
      661 ccatcgatcc ggcgcccttt gaagtggccg ctgcacgagc cgaagccgcg ctcgccagcg
      721 ccgaggcgaa gctcgcccag gcaaagcgca acagcgcacg cctcaagcct ctgttcgaaa
      781 tcaaggccgc gagccagaag gatttcgacg acgcggcttc ggcggagcag gtggccgagg
      841 ccgaggtgaa gaccgcgcgt gccaaccagg cggacgcacg cctcaacctg ggctacaccc
      901 gggtcgaggc gccgattgcc ggcatcgcca gccgcgcgca gcgctccgag gggaactacg
      961 tctccggtcc ggaggtcctg ctgacgacgg tatcgcagat cgatccgatt tacgttctgt
     1021 tcagcgtctc cgacgaggag cgcgtcaggc tcgcccgcga agtcgactcg ggacgcctgg
     1081 tcctgccccg cgacaggcgc ttcgaggtga cggtgaagct cgccgacggc agcgtataca
     1141 gcgaatccgg caagcccaac ttcaccgacg tgcgcgtctc gggcagcacg agcacgagcg
     1201 aggcgcgcgc cgagctgccc aatcccgcgg gcttcctgca ccccggccag ttcgtgcgcg
     1261 tgacgttaaa aggcgccgag cggcccgcca ctatcctggt gccgcagcgc gcggtgctcg
     1321 aaggccccaa aggcaagttc gtgtacgtcg tcaacgccga gagcaaggcc gagtcccggc
     1381 cggtcgagct gggcgactgg cacggcgatg cctggattgt cacggcaggt ctcaacgccg
     1441 gagacagggt gatcgtggac ggcgtgatga aaatcggccc gggcgcaccc gtggccgtcg
     1501 ccgagttgca gccgcgggaa accaaccctg cgaagaacgg gacggccgaa ggccgggctt
     1561 ccgtcgtcgc gcaaaagtaa gggagcttcc acatgttctc tcgttttttc atagatcgcc
     1621 cgatcttcgc ggcggtgctc tcgatcttca tcgtgatcgc cggcctcgcg gcgatgcgca
     1681 accttccgat cgcccagtat cccgagatcg cgccgccggt cgtcaccatc acggcgaact
     1741 acccgggcgc ctcggccggc gtgatcgagc agacggtggc ggcgccgctg gagaaccaga
     1801 tcaacggagt cgagcacatg atctacatga gctcgacctc cacctcgaac ggccaggtgc
     1861 agatccacgt caccttcgat atcggcaccg acatcgaccg ggccacggtc aacatcaaca
     1921 accgcgtcag gcaggtcgag ccgcggctgc ccgcagacgt gcgccggctc ggggtcacgg
     1981 tggaaaaaag ttcctcgtcg ttcctcctgg tggccgcgtt ctactcaccc gacgggcgct
     2041 acgacgatct gtacatcagc aaccacgtca cgatgaacgt gctcgacgtg gtgaagcgca
     2101 ttcccggcac gaccaacgtg cagatcttcg gggcaaagga ttacgcgatg cggatctggg
     2161 tcaagcccga ccgcctcacg cagctcaagc tcacgacggc ggacctgatc gccgcgctga
     2221 acgagcagaa cgcacagttc gccgcgggca aggtcgggca gacgccctca ggcggcccgc
     2281 aggagctcgt ctatacgatc accaccaagg gccggctcgc cga
//
LOCUS       DASPZD010000091         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_441847, whole genome shotgun sequence.
ACCESSION   DASPZD010000091 DASPZD010000000
VERSION     DASPZD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441847"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VEM33_01670"
     CDS             complement(<1..426)
                     /locus_tag="VEM33_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS50566.1"
                     /translation="MEAPQANPEPGAAARRHIIERPRLTRLLDQTQARVIMLVAAAGY
                     GKTTLARQWLEGRAHAWYSITSASSDVAALVLGLARALSDPGDQKFSLLRERLRATRE
                     PAQEVAQLVDLIATSFEAELRNGWIALDDYQALAGSAACE"
     gene            complement(543..809)
                     /locus_tag="VEM33_01675"
     CDS             complement(543..809)
                     /locus_tag="VEM33_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYS50567.1"
                     /translation="MTTMLQASPPVLTPRQKQVVELIAAGYSNEEVGERLGISPRTAK
                     AHCDVLRQKLGVPRRRQIPRAYRELTGDDPLAQSLAPGRSGSGD"
     gene            complement(885..1895)
                     /locus_tag="VEM33_01680"
     CDS             complement(885..1895)
                     /locus_tag="VEM33_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50568.1"
                     /translation="MKGLAELHTHLGGSVSSDILWSLAHEQGIALPVKDYWEFDRLVT
                     IADPRGVADLDALDAIYHWTELIQSSPIAVERSVHAAIGGAYRSQGITTLELRFNPMK
                     RNRGGERDLDHIILAAIRGLDLASLEYPQVNAGLILMMDRTFSQEMNAVIVDKAIRWA
                     PRGICGIDVAGPRPHGERYPYRDLQPLVEEAREAGLGVTIHVGEEGGDAGIEEIGEVI
                     ETLRPDRIGHGILAARDSELMETLRSTQIVLEICPTSNLLTKALESEEAVRETFRAFT
                     DNGVLFTIATDGPEMMRTHLRDEFELLVRIGALEREQLPIANERGHAASFVRREPVLP
                     AA"
BASE COUNT          324 a          730 c          703 g          391 t
ORIGIN      
        1 ttcacaagcg gccgagccgg caagtgcttg ataatcgtcc aaagcgatcc agccatttcg
       61 aagctcagct tcaaatgatg tagcgataag atcgaccaac tgcgccactt cttgcgccgg
      121 ttcgcgggta gcccgaagac gttctcgcag aaggctgaac ttctgatcgc ctggatcgct
      181 caatgctcgt gcaaggccga gaactagcgc agctacgtcg gacgacgctg acgtaatgct
      241 gtaccacgcg tgcgccctgc cttccagcca ttgtcgagca agcgtcgtct tcccgtaccc
      301 ggctgcggcc acgagcatga tgacccgcgc ctgtgtctgg tcaagcagac gcgtaagccg
      361 cgggcgctcg atgatgtggc gccgcgccgc cgctccgggt tccggattcg cctgcggtgc
      421 ttccattgcc caccgggact gactcgcccc gccgacaggt tagtaggcac tgcgacgatt
      481 ttcgaccgtc ggattgagac aagccgcact cgcgaccgcg aacggccacg ggtgcggtgc
      541 cgtcagtccc cgcttccgga acgcccggga gcgaggcttt gcgcgagcgg gtcgtcgccg
      601 gtcagttctc ggtacgcgcg cgggatctgt ctacgccgcg ggacgccgag cttctgcctc
      661 aggacatcgc aatgcgcctt cgccgtcctt ggcgagatgc cgagccgctc tccaacctct
      721 tcgttcgagt acccggccgc gatcaactcg acgacctgct tctgtcgcgg agtcagaacg
      781 ggcgggctcg cttgcagcat cgtcgtcata gccccgacgg taacaagggc gtcctgagtt
      841 ggctattgac caaaaggcgt atttggcccg ctacgccgaa cgcatcacgc ggcgggcagc
      901 accggctcac gccgcacgaa cgaagccgca tgcccgcgct cgttcgcgat cggcagctgc
      961 tcgcgctcga gggcgccgat ccgcaccagc agctcgaact cgtcgcgcag gtgcgtccgc
     1021 atcatctcgg ggccgtcggt tgcgatggtg aacagcaccc cgttgtcggt gaaggcgcga
     1081 aaggtctcgc gcacggcctc ctccgactcg agcgctttcg tcagcaggtt cgaggtcggg
     1141 cagatctcca gcacgatctg cgtcgagcgc agcgtctcca tcagctccga gtcgcgggcg
     1201 gcgaggattc cgtggccgat ccggtcgggg cgcagggtct cgatcacctc gccgatctcc
     1261 tcgatgccgg cgtcgccgcc ctcctctccg acgtggatcg tgacgcccag cccggcctcg
     1321 cgcgcctctt cgacgagcgg ctgcagatcc cggtagggat agcgctcgcc gtgcgggcgc
     1381 gggccggcga cgtcgatccc gcagatcccg cgcggcgccc agcggatcgc cttgtcgacg
     1441 atgaccgcgt tcatctcctg gctgaaggtg cggtccatca tcaggatcag gccggcgttg
     1501 acctgcgggt actcgaggct ggcgaggtcg agcccgcgga tcgcggccag gatgatgtga
     1561 tccagatcgc gttcgccgcc ccggtttcgc ttcatggggt tgaagcggag ctcgagcgtc
     1621 gtgattccct gcgagcggta ggcgccgcca atcgcggcgt gcaccgagcg ctcgacggcg
     1681 atcgggctcg actggatcag ctccgtccag tggtagatcg cgtcgagcgc gtccaaatcg
     1741 gcgaccccgc gcgggtcggc gatggtcacc agccggtcga actcccagta gtccttgacc
     1801 ggcagcgcga tcccctgctc gtgcgcgagg ctccagagga tgtccgagga aacggacccg
     1861 ccgaggtgcg tgtgcagctc ggcgaggccc ttcatggacg aagcctagcg gcgctctgct
     1921 cgctccttca ggcgttcatc tggcgaagat cggccgattt atctcaatcg ggcgcggcct
     1981 tagcgggccg ctatgtcttg gcgatcgcgc tggcgatcgc cgtcggcgtc gtcgtcgaca
     2041 tcaggatcga cctcgcgggc tcgtgccgca tgctcttcgg cggcggcgcg ttcgcgcttg
     2101 gcccgttggg catgctcctc cgcggcgagc tcggcctgct ttgcgctc
//
LOCUS       DASPZD010000092         3991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_317365, whole genome shotgun sequence.
ACCESSION   DASPZD010000092 DASPZD010000000
VERSION     DASPZD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317365"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..789)
                     /gene="kdsB"
                     /locus_tag="VEM33_01685"
     CDS             complement(16..789)
                     /gene="kdsB"
                     /locus_tag="VEM33_01685"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HYS50569.1"
                     /translation="MSFTAIIPVRFASSRLPGKALADIGGRPMVVRVADRARESGARD
                     VYVATDHGDIAAAVERYGHRAVMTRGDHATGTDRIAEVVERLGLAADSLVVNVQGDEP
                     MIEPGLIRAVATLLEDTPEAAMATACHPIRDARELFNPNVVKVVLDHAGMARYFSRAP
                     VPWARDAFGDSTQALPQGLPCYRHIGIYAYRADFLRSYPRLERSPLEEFEALEQLRAL
                     WHGYAIAVALRQDAPLAGVDTIEDLERTRSLFDRTVHKR"
     gene            complement(819..1004)
                     /locus_tag="VEM33_01690"
     CDS             complement(819..1004)
                     /locus_tag="VEM33_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HYS50570.1"
                     /translation="MDSRLLDILVCPICKGPLVYRRAQAEFICKADRLAFPVRDDIPV
                     MLEEEARKLPPEEEVSE"
     gene            complement(985..1992)
                     /gene="lpxK"
                     /locus_tag="VEM33_01695"
     CDS             complement(985..1992)
                     /gene="lpxK"
                     /locus_tag="VEM33_01695"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HYS50571.1"
                     /translation="MEAARPIFLAAGFAARHWYRLSAVSLLLFPLSLVFRLAVALRRL
                     LFRAGALPSVRLRVPVIIVGNLTVGGTGKTPLILALVDALRAKGLNPGILSRGHGGND
                     TGPHAVSERDDAAQVGDEPLLLAERSGCPVWIGADRAAAARALLAAYPRCDVILCDDG
                     LQHYRLQRDFEIAVEDERGFGNGLLLPAGPLREPAGRRVDATVVNGAEPKPGAFRMRL
                     APAGLYRVDGHGAPLAQSELSGKKLHAVAGIGNPERFFDGLSRMGLEFSAHPFPDHHA
                     FRAADLEFADCDFVLMTEKDAVKCRHFGRRDLIAVRVEAELDSALAELILERIHGFAA
                     A"
     gene            complement(2007..2423)
                     /locus_tag="VEM33_01700"
     CDS             complement(2007..2423)
                     /locus_tag="VEM33_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYS50572.1"
                     /translation="MRFARTRKEDPEINLIPMIDVLLVIIIFLMLTTTYSRFAELKIN
                     LPSADAEKKLERSNEINVVISANGQYLLQRKPLQFRNVATLAEELRRAGAGMKEAIVV
                     VNADNNANYQSVIRVMDAARQAGFGQVVFAVEQSTK"
     gene            complement(2420..3052)
                     /locus_tag="VEM33_01705"
     CDS             complement(2420..3052)
                     /locus_tag="VEM33_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYS50573.1"
                     /translation="MFAIIHAAGWPIYFLLAVSVIAVALIIERFMILRKEKIVPGGLL
                     ERVLVAYQKQGVSEDMLEKLSQDSPLGQVLASGLRNYKSSRDVMKDAIEEAGSAVAHE
                     LERFLTTLGTIATISPLMGLFGTVVGMIEIFGSQSPTGSNPQELAHGISVALYNTGFG
                     LVIAIPAMIFFRHFRGLVEGFVVEMEQQAAKLVDAVHGERFEFQPPHTRV"
     gene            3434..>3991
                     /gene="xseA"
                     /locus_tag="VEM33_01710"
     CDS             3434..>3991
                     /gene="xseA"
                     /locus_tag="VEM33_01710"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HYS50574.1"
                     /translation="MPGEQGLARVSTHSPPEILSVSALVRSVRDLLERRYPLLWVAGE
                     ISNFTVAKSGHAYFVLKDEHAQVRCVMFRQRQQNLEWTPRDGMQIEVQALVSLYEPRG
                     EFQLTVETMRRAGLGARFEAFVKLRDRLEKEGLFDAETKRALPSLPRAIGVITSREAA
                     ALGDVLTTLARRNPSIEVVVFPVQVQ"
BASE COUNT          755 a         1280 c         1241 g          715 t
ORIGIN      
        1 cccgacgcgg cgaccttacc gcttatgaac cgtgcggtca aacagggacc gtgtgcgttc
       61 cagatcctcg atcgtgtcga cgccggcgag cggcgcatcc tgtcgcaatg cgacggcgat
      121 cgcgtagccg tgccagagcg cccgcagctg ctcgagcgct tcgaactcct cgagcggcga
      181 gcgctcgagg cgcggatagc tgcgcaggaa atcggcgcga taggcataga tgccgatgtg
      241 ccggtagcag ggaagcccct gaggcagggc ctgtgtggaa tccccgaagg cgtcccgggc
      301 ccagggcacc ggtgcccggc tgaaatagcg cgccattccg gcatggtcca gcacgacttt
      361 gacgacgttg ggattgaaaa gctcgcgcgc gtcccggatc gggtggcagg ccgtggccat
      421 ggccgcttcc ggagtatcct cgagcagcgt cgccaccgca cgtatcaggc ccggctcgat
      481 catcggctca tcgccctgga cattgacgac cagactgtcc gccgcgaggc cgaggcgctc
      541 cacgacctcc gcgatccggt cagtcccggt cgcgtgatcg cctcgtgtca tgacggcccg
      601 gtggccgtac cgttctacgg cggccgcgat gtcgccgtgg tcggtcgcta cgtatacgtc
      661 gcgcgccccg ctttcgcgcg cccggtcggc tactcgcacg accatgggcc tgccgccaat
      721 atcggcgagc gcctttccgg gaagccggct ggacgcgaat cgcaccggga taatggcggt
      781 gaaggacatg cgaaggattc taggcgctgc ctccccgttt actcgctcac ttcttcctcg
      841 gggggcagct ttctcgcctc ctcctcgagc atcaccggaa tatcgtcccg aaccggaaac
      901 gcgaggcggt cggccttgca gatgaattcc gcttgcgccc ggcggtagac cagtggccct
      961 ttgcaaatcg ggcagacgag aatgtcaagc agccgcgaat ccatgaattc gttccagtat
     1021 gagttccgcc aaggcagagt cgagttcggc ctcgactcgc accgcaatca gatcgcgccg
     1081 gccgaagtgg cggcatttta ccgcatcctt ttcggtcatc agcacgaaat cgcaatcggc
     1141 gaactcgagg tctgcagccc gaaacgcgtg gtgatccgga aacgggtgag cagagaactc
     1201 gagccccatg cgcgaaagcc cgtcgaaaaa ccgctcggga ttgccgattc cggcgaccgc
     1261 gtgcagtttt tttccggaaa gctccgactg cgccaaaggc gccccgtgcc catcgacccg
     1321 gtacagaccc gccggcgcga gccgcatgcg aaatgcgccc ggcttcggct cggcgccgtt
     1381 cacgacggtc gcatccacgc ggcgaccggc aggctcacgc aaaggaccgg ccggaaggag
     1441 caagccgttg ccaaaaccgc gctcatcctc gactgcgatc tcgaaatctc gctgcaagcg
     1501 gtaatgctgc aatccatcgt cgcagagaat cacgtcgcag cgagggtagg ctgcgagcag
     1561 cgcacgcgcg gcggcggcgc gatctgcgcc gatccacacc gggcaccccg aacgctctgc
     1621 gagcagcaga ggctcgtcgc cgacttgcgc ggcatcatca cgctcgctca ccgcgtgggg
     1681 tccggtgtcg ttcccgccgt ggcctcggct gagaatcccg gggttcagcc ccttcgcgcg
     1741 caaggcgtcg acgagcgcga ggatcagcgg ggttttcccc gtgcctccga ccgttaggtt
     1801 tccgacgatg atcaccggaa cgcggagtcg caccgagggc agcgcacccg cgcgaaagag
     1861 cagacggcgc agggccaccg ccagacgaaa gacaaggctc agcggaaaca gcagcagcga
     1921 taccgcgctc agccggtacc agtggcgcgc ggcaaagcca gcggcgagaa aaatgggccg
     1981 ggcggcctcc atacgcgcgc cgcgcgctac ttcgtcgatt gctccacagc gaacacgacc
     2041 tggccaaagc ccgcctggcg cgcggcgtcc atgacgcgga tcaccgattg atagttggca
     2101 ttgttgtcgg cgttgacgac cacgatcgcc tccttcatgc cggcgccagc gcgacggagc
     2161 tcttcggcga gggtcgcgac gttccggaac tggagcggtt ttcgctgcag caggtattgc
     2221 ccgttggcgc tgatcaccac gttgatctcg ttggatcgtt ccagcttttt ctccgcgtcg
     2281 gcgctcggca ggttgatctt cagctccgca aacctcgagt aggtcgtagt cagcatcagg
     2341 aagatgatga tcacgaggag cacgtcgatc atcggaatca ggttgatttc cgggtcttcc
     2401 ttccgcgtgc gggcaaacct catacccgag tatgcggcgg ctggaattcg aagcgttctc
     2461 cgtgcaccgc gtcgaccagt ttcgccgcct gttgctccat ctcgaccacg aagccttcga
     2521 ccaagccgcg gaaatggcgg aagaaaatca tcgctggaat ggcgatcacc aggccgaatc
     2581 ccgtgttgta cagtgccacg gaaatgccgt gggcgagctc ctgggggttg gatccggtgg
     2641 gcgattgcga gccgaagatc tcgatcatgc cgaccacggt gccgaacaag cccatcagcg
     2701 ggctgatcgt tgcgatcgtc ccgagcgtcg tcaagaagcg ctccagctcg tgcgccaccg
     2761 cgctgccggc ctcttcgatc gcgtccttca tcacgtcgcg cgaactcttg tagttgcgaa
     2821 ggcccgaggc cagtacttgg ccgagcggag agtcctgcga gagcttctcg agcatgtcct
     2881 cgctcactcc ctgtttctgg tatgcgacga gcaccctctc cagcaggccg cccggaacga
     2941 tcttctcctt gcgaagaatc atgaaccttt cgatgatgag cgccactgcg atgaccgaca
     3001 cggcgagtag gaaatagatc ggccagccgg cagcatggat gatcgcaaac aaccgcgttc
     3061 tccgagtctg acaaaaaacg cggacacttt agcctgcgac cgcagattcg gcaaggcaaa
     3121 gaatttcaag aagatgcgcc tctcgcgctc aaaaaatccg gcgacgggag cattgcgcgc
     3181 cgcacgcgcg acgcgcgcaa acgggctcga agtctttatc cacaaatact gtggataagg
     3241 ttgtgcatac tcgccaaata gcgagcgtaa gtgaatcgcg caaaagggtt ttcctgttca
     3301 tgatgaaaac ttgaccttcg atttttcact ttaccgctca aagagttgca ggcatgttct
     3361 cgggcattgt ctgaaccgct cataggggac catattggcg gagagcccgt tgtggatagg
     3421 ttacaatcgg cgcatgcccg gagaacaagg actggcgcgg gtttccacgc attccccccc
     3481 ggaaattctc agcgtttcgg ccctcgttcg cagcgtccgg gatctgctcg agcgtcgcta
     3541 tccgctgctt tgggtcgccg gcgagatatc caacttcacc gttgccaagt cgggacacgc
     3601 gtatttcgtt ctgaaggacg agcacgctca agtgcgctgc gtcatgttcc ggcagcgcca
     3661 gcagaatctc gagtggacgc cgcgcgacgg catgcagatc gaggtgcagg cgctcgtcag
     3721 cttgtacgag ccgcgcggcg agttccagct caccgtcgaa accatgcgcc gcgcgggtct
     3781 cggcgcgcgc ttcgaggcgt tcgtcaagct gcgcgacagg ctcgagaaag aaggcctgtt
     3841 cgatgccgaa acgaaacgag cgctgccttc cctgccgcgc gcgatcggcg tcatcacttc
     3901 gcgcgaggcg gcggcgcttg gcgacgtatt gacgacgctg gcccggcgca acccgtcgat
     3961 tgaggtggtc gtctttcccg tgcaggtcca g
//
LOCUS       DASPZD010000093         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_557479, whole genome shotgun sequence.
ACCESSION   DASPZD010000093 DASPZD010000000
VERSION     DASPZD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557479"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VEM33_01715"
     CDS             complement(<1..344)
                     /locus_tag="VEM33_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50575.1"
                     /translation="MHARSVTRAESAELEACGESRLGRRFGAFCRLLRSLGSKRRKLI
                     GTEQSDCLRVELLLHQLRLVRCAAGAKIDPSEKTEIGEQQQEGYGFHDFPWVISPRPV
                     QFGPSLPAPGCRR"
     gene            393..767
                     /locus_tag="VEM33_01720"
     CDS             393..767
                     /locus_tag="VEM33_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYS50576.1"
                     /translation="MEEIASYWVFMPPQRNRQAANQKAAELKKLGVEDFYVVQEDSKF
                     RFAISLGVFKTEEAARSRLAELRKKGVRTARVDPRETSVQKVYFSVREVPDALVSRLN
                     DLRQNFAGSELKACPPEEKRSR"
     gene            complement(764..1243)
                     /locus_tag="VEM33_01725"
     CDS             complement(764..1243)
                     /locus_tag="VEM33_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HYS50577.1"
                     /translation="MEVPFEKKICPPQQLAVRLGSRARPLVFTNGVFDILHRGHVTYL
                     AEASSLGATLLVAVNSDASAKRLGKGEERPLNPLADRMAVVAALESVHLVTWFDEDTP
                     LALILASRPEVLAKGGDWKPEAIVGAAEVRGWGGSVHSIPFRHERSTTALLRKILGR"
     gene            1312..1863
                     /locus_tag="VEM33_01730"
     CDS             1312..1863
                     /locus_tag="VEM33_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HYS50578.1"
                     /translation="MRILLGLLLAASIPAPALAACPPVLNHTLPSLTDQATPLCQFEG
                     KVVLVVNTASQCGYTPQYEGLEGLYRRYRDKGLVVLGFPANDFGGQEPGGNRDIAKFC
                     EVNYGVSFPMFAKSGVSADNANPLYATLAAKTGERPRWNFHKYLIDRSGEKVLSFGSG
                     VTPGDRKLVGEIERMLAASERKP"
     gene            complement(1886..2491)
                     /locus_tag="VEM33_01735"
     CDS             complement(1886..2491)
                     /locus_tag="VEM33_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYS50579.1"
                     /translation="MKTCPAARRYAAVALIFASAAILQACVDALIIGGVATGVVLAAD
                     RRQAEVMYTDQRIEFAAGSRIGEALKDQGHVNVTSYNYTVLVTGEVPTAQAKAEVEKI
                     VSEIPQVKTVVNELQIAGTSSAASRSNDAYITSKVKGNFLGNGKVRPTDVKIVTEAGV
                     VYLLGLVTREEADAATEVARGTGGVQKVVRVFEYVTPPAKK"
     gene            complement(2488..>2616)
                     /locus_tag="VEM33_01740"
     CDS             complement(2488..>2616)
                     /locus_tag="VEM33_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HYS50580.1"
                     /translation="LLASEDVHICVPHSQTARIQEVHLLTLHCLCDGIDCMLLGAE"
BASE COUNT          479 a          832 c          811 g          494 t
ORIGIN      
        1 cgtcgacaac ctggtgctgg aaggcttggt ccgaattgca caggacgcgg tgagatgacc
       61 cacgggaaat cgtgaaaacc gtaaccttcc tgctgctgtt cgccaatctc ggttttttcg
      121 cttggatcta ttttggcgcc ggccgcgcaa cggacgagcc gcagctgatg gagcagcagc
      181 tcaacccgca ggcaatccga ttgctccgtg ccgatcagct tgcggcgctt gctgccgagc
      241 gaacgaagca gacggcagaa cgccccaaac cgcctcccaa ggcgactctc gccgcatgcc
      301 tcgagctcgg cgctttcagc ccgggtgacg ctgcgcgcgt gcaacaggct ctcgaacctc
      361 ttgccctcgg gtcgagactc tcgcagcgcc gtgtggaaga gatcgcgagc tactgggtat
      421 tcatgccgcc ccagcgcaat cggcaagcgg caaaccagaa agccgccgaa ctcaagaagc
      481 tcggcgtcga agacttctat gtcgtgcagg aagattccaa gttccgattt gcgatttctc
      541 tgggcgtgtt taaaacggaa gaggcagcgc ggtcgcggct cgcggagttg cgcaaaaagg
      601 gcgttcgaac ggcccgggtc gatccccggg aaacatccgt gcagaaagtc tacttcagcg
      661 ttcgtgaggt ccccgacgcg ctggtttcga gactgaacga cctgcgccag aactttgccg
      721 gcagcgagct gaaggcctgt ccgccggagg aaaaacgcag ccgctagcgt ccgaggattt
      781 tcctgagcag cgcagtcgtg gagcgctcgt gcctgaaggg gatcgaatgc acgcttccgc
      841 cccagccccg cacttcggcg gctccgacta tcgcttcggg cttccagtcg ccgcccttgg
      901 cgagcacctc cggccgggag gcaagtatca gcgcgagggg cgtgtcctcg tcgaaccacg
      961 tcacgagatg gacggattca agcgccgcta ccaccgccat gcggtctgcg agcgggttga
     1021 gcggacgttc ctcgcctttg cctagacgct tcgcagatgc gtcgctgttg actgcgacga
     1081 ggagggtcgc cccaagggag ctcgcttcgg cgagataggt gacgtgcccg cgatgcagga
     1141 tgtcgaacac accattggtg aataccaggg gccgcgcacg ggagccgagc cgcaccgcaa
     1201 gctgttgcgg cgggcagatc ttcttctcga aagggacctc catctggcct attttgacag
     1261 aacgacccgc cggctgtaac ttaggccgac ctcagccaca ggttcccgcc gatgcgcatt
     1321 ctcctcggac tgttgctcgc cgcttcgatc cccgcgcccg cgctcgccgc gtgcccgccg
     1381 gtgctcaacc ataccctgcc gagcctgacc gatcaggcca cgccgctgtg ccaattcgag
     1441 ggcaaggtgg tgctcgtggt gaataccgcg agtcaatgtg gttacacgcc tcagtacgaa
     1501 ggactggagg ggttgtaccg ccgctaccgc gacaagggcc ttgtcgtgct gggcttcccc
     1561 gccaacgatt tcggcggcca ggagccgggg ggcaatcgcg acatcgccaa attctgcgag
     1621 gtcaactacg gcgtaagctt tccgatgttc gcgaaatctg gcgtgtcggc ggacaacgcc
     1681 aaccccttgt acgcgacgct tgccgcgaag accggcgagc gcccccggtg gaatttccac
     1741 aagtacctga tcgaccggtc cggcgagaag gtgctgtcct tcggcagtgg agtaacaccc
     1801 ggcgaccgca agctggtcgg cgagatcgag cggatgctcg cagcctccga acgcaaaccc
     1861 tagacagggg gcgttggacc cgttcctatt tcttcgcggg cggggtgacg tactcgaaaa
     1921 cgcggacgac tttctgcacc cctccggtgc cgcgcgcgac ttcggtcgcg gcgtccgctt
     1981 cttcacgcgt gaccagtccg agcaaataca cgacccctgc ctcggtcacg attttgacat
     2041 cggtgggccg aaccttcccg tttccgagaa agttgccttt gaccttgctg gtgatgtagg
     2101 cgtcgttgct gcgcgaagcg gccgagctcg ttcccgcgat ctgcagctcg ttgacaacgg
     2161 tcttcacctg gggtatttcg ctcacgattt tttccacttc cgcttttgcc tgcgcagtgg
     2221 gcacttcgcc ggtgaccagc accgtgtagt tgtagctggt gacgttgacg tgtccctggt
     2281 ccttgagcgc ctcgccgatg cggctgcccg cggcgaattc gatccgctgg tcggtgtaca
     2341 tcacttcagc ctggcgacga tcggcggcga ggaccacccc cgtagcaacc ccgccgataa
     2401 tgagtgcgtc gacgcaggcc tggaggatcg cggccgaagc gaaaatcagc gccaccgccg
     2461 cgtatcgcct tgctgccgga caggttttca ttcggccccc aacaacatgc aatcgatgcc
     2521 gtcgcaaagg cagtgcagag tcaggagatg cacttcctga atgcgggcgg tttgcgagtg
     2581 gggcacgcag atatgcacgt cttccgaagc gagcag
//
LOCUS       DASPZD010000094         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1184022, whole genome shotgun sequence.
ACCESSION   DASPZD010000094 DASPZD010000000
VERSION     DASPZD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184022"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VEM33_01745"
     CDS             complement(<1..1009)
                     /locus_tag="VEM33_01745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYS50581.1"
                     /translation="MSAAQHESDEIAAIRQAVRALCEKFPGEYWRALDRERAYPTEFV
                     EALSGAGFLAALIPEQYGGSGLSMGAAIAIMEEIQANGCNGAACHAQMYVMGTLLRHG
                     SPEQKQKFLPSIARGELRLQAFGVTEPTSGTDTLNLRTTAVRDNDHYVVNGQKIWTSR
                     VEHSDLMLLLARTTPREQAQKRTEGLSVFLVDMREARGRGLTIRPIRTMMNHATTEVF
                     FDDLRVPAENLIGREGQGFHYILSGMNAERILIAAECIGDAKWFIGRASGYARERNVF
                     GRPIGQNQGVQFPIARAYAQMRGAELMVHEAAGLYEAGRDCGAEANMAKLLAADASWA
                     AA"
     gene            complement(1179..2282)
                     /locus_tag="VEM33_01750"
     CDS             complement(1179..2282)
                     /locus_tag="VEM33_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYS50582.1"
                     /translation="MQRTKPPYRADHVGSLLRSAPLKQARAARERGEISAAGLREIED
                     REIEKIVRKQQRIGLKLATDGEYRRSWWHFDFLEKLDGVEGFSPEHGIQFHGVETKPK
                     AVRVVGKIRFSGHPMLEHFKFLQAHARVQPKMTIPSPSVLHFRSGRTGISKQIYPDIE
                     GFFDDLAEAYRKAVRAFADAGCRYLQLDDTVWAYLCSAEQREEARQRGDDPQTLPATY
                     ARVINHAIAGRPSDMVITTHSCRGNFRSTWISEGGYEPVAEVLLGQVNYDGYFLEYDT
                     DRAGGFEPLRFFPKGKKQLVLGLVTSKSGTLEHKDDVKRRIEEATKYVDLDQLCLSPQ
                     CGFASTEEGNILAEDEQWAKLAMIVEIAEEVWG"
BASE COUNT          356 a          781 c          761 g          443 t
ORIGIN      
        1 cggccgcggc ccaggacgcg tcggcggcga gcagcttcgc catattggct tccgcgccgc
       61 agtcgcggcc ggcctcgtaa agccccgcgg cttcgtgcac catcagctcg gcgccgcgca
      121 tctgcgcata ggcgcgcgcg atcgggaatt gcacgccctg attctgcccg atcggccgtc
      181 cgaacacatt gcgctccctg gcgtagccgc tcgcccgccc gatgaaccat ttggcatcgc
      241 cgatgcattc ggccgcgatc aggatgcgct cggcgttcat gccggacaga atgtaatgga
      301 agccctgccc ttcgcggccg atcagattct cggccggcac gcgcagatcg tcgaagaata
      361 cttcggtggt ggcatggttc atcatggtgc ggatcggccg gatggtgagg cccctgcccc
      421 ttgcctcgcg catgtcgacc aggaacaccg agagcccttc ggtgcgtttt tgcgcctgct
      481 cccgcggcgt ggtgcgggcg agcagcagca tcaggtcgga atgctcgacc cgcgacgtcc
      541 agattttctg gccgttgacg acgtaatggt cgttatcgcg gaccgcggtg gtgcgtagat
      601 tcagcgtgtc ggtgccagag gtcggctcgg tcacgccgaa ggcctgcagc cgcaattcgc
      661 cgcgcgcgat ggagggtagg aatttctgct tctgctccgg gctgccgtga cgcagcagcg
      721 tgcccatcac gtacatctgg gcgtgacagg cggcgccgtt gcagccgttc gcctggatct
      781 cctccatgat ggcgatcgcc gcgcccatgg aaagcccgct gccgccgtat tgctcgggga
      841 tcagcgcggc caggaacccg gccccggaca gcgcctcgac gaactcggtc ggataggcgc
      901 gctcgcgatc gagcgcgcgc caatattcgc ccgggaattt ttcgcacagc gcccgcaccg
      961 cctggcgaat cgcggcgatc tcgtcggact cgtgctgcgc ggcactcacg gcgcttcacc
     1021 ctcccctgga gggggagggt cgcgcgcgaa gcgcgcgggg tggggtgatt cagcttcacc
     1081 cccacccggc tcgcgtctga cgacgctcgc cgacctcccc cctccagggg gaggtggagc
     1141 catgccccgc atgcatcgac tcatccctgc actcaccctc atccccacac ttcctcggcg
     1201 atttcgacga tcatggcgag cttcgcccat tgctcatcct cggccaaaat attgccctcc
     1261 tcggtcgagg caaagccgca ttgcggggag aggcagagct ggtcgaggtc gacgtatttg
     1321 gtcgcttcct cgatccgccg cttgacgtcg tccttgtgct cgagcgtgcc gctcttcgag
     1381 gtgacgaggc cgagcacgag ctgcttcttg cctttgggaa agaaccgcag cggctcgaaa
     1441 ccgccggcgc ggtcggtgtc gtattcgagg aaatagccgt cgtaattcac ctgtccgagc
     1501 agcacttcgg cgaccggctc gtagccgccc tccgagatcc aggtcgagcg gaaattgccc
     1561 cggcacgaat gggtggtgat caccatgtcg ctgggccgtc ccgcgatcgc gtgattgatg
     1621 acacgggcgt aggtcgccgg cagcgtttgt ggatcatcgc cgcgttggcg cgcctcctcg
     1681 cgctgctcgg cggagcacag ataggcccac accgtgtcgt cgagctgaag atagcggcag
     1741 ccggcatccg cgaacgcgcg caccgccttg cggtaggcct cggcgagatc gtcgaagaat
     1801 ccctcgatat ccgggtagat ctgcttgctg atgccggtgc ggccgctgcg gaaatgcagc
     1861 acgctcgggc tcggaatggt catcttcggc tgcacgcgcg cgtgcgcttg cagaaatttg
     1921 aaatgctcga gcatcgggtg gcccgagaag cggattttgc cgacgacgcg cacggccttc
     1981 ggtttggtct cgaccccgtg gaactggatg ccgtgctcgg gcgaaaagcc ttcgacgccg
     2041 tcgagttttt ccagaaaatc gaaatgccac cacgagcggc gatactcgcc gtcggtcgcg
     2101 agtttcaacc cgatccgctg ctgcttgcgg acgatcttct cgatttcgcg atcctcgatt
     2161 tcgcgcaatc cggcggcgga aatctcgccg cgctcgcgcg cggcgcgcgc ctgcttgagc
     2221 ggcgcggagc gcagcaggct gccgacgtgg tcggcgcggt agggcggctt cgttctctgc
     2281 atggctccgt cctgtcgcct cctgttcacg gcaatggcgc gcggcgccct tgcgatttgc
     2341 c
//
LOCUS       DASPZD010000095         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_439801, whole genome shotgun sequence.
ACCESSION   DASPZD010000095 DASPZD010000000
VERSION     DASPZD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439801"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..585)
                     /locus_tag="VEM33_01755"
     CDS             complement(214..585)
                     /locus_tag="VEM33_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS50583.1"
                     /translation="MAGKKILIVDDSATERYFFMETLSKQGYECITAQNGDEAIQKSR
                     SEQPDVILMDIVMPGIDGYKATRTISRDEATKHIPVIICTSRNQETDRVWGMRQGAKD
                     FLVKPVRAKDLIAKIAATAAG"
     gene            complement(754..2382)
                     /locus_tag="VEM33_01760"
     CDS             complement(754..2382)
                     /locus_tag="VEM33_01760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYS50584.1"
                     /translation="MRLPRFARMADPWFRDGFLRYRVASVAVIVLGISLSVAAFLAIG
                     SNERDVVRLTFEQRAQSDVAAINLAMERGLESVENSGALFDAGNEITSAQFQAFGERA
                     LARNPALKAVAWTPFVSANMRRAFEAAAQKDQPGYRVKELSAQFKLVLAGERDEYFPI
                     LYSVDRDGPGQSPFGMDRLSVANSREGLERSRTSGATTSSGRIRITTGQFQGEMGVGL
                     SRPVYRRGVPLDTAEQRSRNLLGFTISIVVIRDLIDNVLKRIGSEGIRVVLYDESARE
                     SERFLAVWPDIAQKDPALDEKQAPALQRRAEFTVGDRNWVAVMTPEAGRFSTGASWRA
                     WAVAGTGLLFTLLLAVYVESARRHARSIHEQAITDSLTGLHNRRYLWELLKREFARAR
                     RNRSTIAAVMIDIDHFKRINDNFGHEAGDLVIAELGALFKRSVRGSDVACRYGGEEFA
                     LVLPEISIENARRKAETLRQGVKSLALEHRGTPLGPITTSLGLAVFPNHAEDAESLFR
                     RADQALYEAKNAGRDRVVVAQSAPPGGELILAPA"
     gene            complement(2414..>2621)
                     /locus_tag="VEM33_01765"
     CDS             complement(2414..>2621)
                     /locus_tag="VEM33_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50585.1"
                     /translation="VERDVGAFEQRFRAAAERDLGEADHVGSIGKARIVRATVGQSGE
                     NSTRFEFAQPGLARPAASRAGGVK"
BASE COUNT          445 a          882 c          830 g          464 t
ORIGIN      
        1 gaggccggcc ggcgggcagg tagccccaga cgaccgtggc ggaattggat gggacgtgcg
       61 catcgacccg gtgcggggcc ggccacatgg aggcgcagcc gaagccggcg agcacgcacg
      121 gaatgaggat caggcgagcg agcttcatgg ggaccgaccc attatagcct cgcacagccg
      181 aagaattgcc ggcgggcgcg ccggcgtggg agcctagcct gccgccgtgg ccgcgatctt
      241 cgcgatcaaa tccttcgccc tcaccggctt caccagaaag tccttcgcgc cctggcgcat
      301 tccccagacg cggtcggttt cctggttccg gctcgtgcag atgatgaccg gaatgtgctt
      361 ggtcgcctcg tcgcgggaga tggtccgcgt cgccttgtag ccgtcgatgc cgggcatgac
      421 gatatccatc aggatcacgt cgggctgctc gcttctggat ttctggatgg cctcgtcccc
      481 gttctgggcg gtgatgcact cgtagccctg cttggagagc gtttccatga agaaataacg
      541 ctcggtggcc gaatcgtcga cgatcaggat cttcttcccc gccatggcaa tctcctcgtg
      601 gagccgctaa ataatcagcc gcaggacatg ccgcaagcgt cgaaagagcg tcggcggcgg
      661 cgagtcgtcg ccatttccgc cctccccgac ccttgcgacc tggtccccag ctctacgagc
      721 aagaccgatg ccagacgcgc cgcagacccc gcgtcaggcc ggagcgagga tcagttcgcc
      781 gcccggaggc gcggactgcg cgacgacgac gcggtcgcgg ccggcgttct tcgcttcgta
      841 caaggcctgg tcggcccggc gaaagagcga ttccgcgtcc tcggcgtgat tgggaaacac
      901 cgcgaggccg agcgaggtgg tgatcggccc cagcggcgtg ccgcgatgct cgagcgcaag
      961 gctctttacc ccctgccgaa gcgtctccgc ctttcgccgc gcgttctcga tcgagatctc
     1021 cggcaggacg agcgcgaact cctcgcctcc gtagcggcac gccacatcgc tgccgcgcac
     1081 gctgcgcttg aacagcgcgc ccagctcggc gatgaccagg tctccggcct cgtgtccgaa
     1141 gttgtcgttt atccgcttga agtggtcgat gtcgatcatg acggcggcga tggtgctgcg
     1201 gttgcgacgg gccctcgcga actcgcgctt cagcagctcc cacaggtagc gccggttgtg
     1261 caagcccgtg agcgaatcgg tgatcgcttg ttcatggatg ctccgtgcat gcctgcgcgc
     1321 ggattccacg tagacggcga gcagcagcgt gaagagaagt ccggtgcctg ccaccgccca
     1381 ggcccgccag gatgcgccgg tgctgaacct tcccgcttcc ggggtcatca cggcaaccca
     1441 gttgcgatcg cccactgtga actcggcgcg gcgctgcagc gcgggcgctt gcttctcgtc
     1501 cagggcggga tctttctggg cgatatcggg ccagacagcg agaaagcgct ccgattcgcg
     1561 cgccgattca tcgtaaagca cgacgcggat gccttcggag ccgatccgtt tcagcacgtt
     1621 gtcgatcagg tccctgatca cgacgatgga aatcgtaaac ccgagcagat tgcggctgcg
     1681 ctgctcggca gtatcgagcg gcacgccccg gcgatacacc ggccggctga gccccactcc
     1741 catctcgccc tggaactggc cggtggtgat ccggattctt ccgctggaag tcgtggcacc
     1801 cgaggtcctc gaccgctcga gcccctcgcg gctgtttgca accgagagcc ggtccatccc
     1861 gaaggggctc tggcccggtc cgtcccggtc caccgagtac aggatcggaa aatattcgtc
     1921 gcgctcgccc gccaagacca gcttgaactg cgcgctcaac tccttgaccc ggtatccggg
     1981 ctggtccttc tgcgccgccg cctcgaatgc gcggcgcatg ttcgccgaca cgaacggagt
     2041 ccaggcgacc gctttcagcg caggattgcg ggcgagcgcc cgctcaccga acgcctggaa
     2101 ttgcgcgctt gtgatctcat ttccggcatc gaacaaggcg cccgaattct cgaccgactc
     2161 caggccccgt tccatcgcca ggttgatggc agcgacgtcg ctctgagccc tctgttcgaa
     2221 ggtgagccgc accacgtcgc gttcgttgct tccaatcgca agaaacgcgg caacggaaag
     2281 tgagatcccg agcacgatga ccgcgacgga agctacgcga tagcggagaa atccgtcgcg
     2341 gaaccagggg tctgccatcc tcgcgaagcg cggcaatctc atcgaagaac tctacccgga
     2401 tcggctggcg cgcttatttt accccaccgg cgcgcgaggc tgcggggcgg gcgagaccgg
     2461 gctgcgcgaa ttcgaacctt gtcgaatttt cgcctgattg gccgaccgtt gcgcgtacaa
     2521 tgcgcgcctt gccaattgaa ccgacatgat ccgcttctcc caggtcacgc tccgccgcgg
     2581 cgcgaaaacg ctgctcgaag gcgccgacgt ctcgctcaac c
//
LOCUS       DASPZD010000096         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1642540, whole genome shotgun sequence.
ACCESSION   DASPZD010000096 DASPZD010000000
VERSION     DASPZD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642540"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VEM33_01770"
     CDS             <1..766
                     /locus_tag="VEM33_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYS50586.1"
                     /translation="EGCGRHRIRGISPWRDKLAEMGAKEAAKAIRDSGLVVTGLCRGG
                     MFPGADRRARQAAIDDNLRAIDDAATLEARCLVLVVGGLPLGSKDLAGARAMVRDGIG
                     GVLDHARSAGVPLAIEPLHPMYCADRACVNTLAQALELCDELDGSGSGALGVVVDAYH
                     VWWDPSLKTQIERAGDKRLLAFHICDWLVPTADLLTDRGMMGDGVIDLPLIRAWMEEA
                     GYRGFHEVEILSSRNWWRRDPDEVLASCRVRHQTAC"
     gene            865..1890
                     /locus_tag="VEM33_01775"
     CDS             865..1890
                     /locus_tag="VEM33_01775"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HYS50587.1"
                     /translation="MDRNLLSKTAGAMVARGKGLLAADESSGTCEKRFKSVGVECTEA
                     NRRAYRDLLFTTKGIEEFVSGVILFDETLRQSTVSGNEPFPRYLEKKGVIPGIKVDKG
                     THDLALSPGEKVTEGLDGLRGRLEDYFGLGARFAKWRAVITIGAGIPSHACIYANAHA
                     LARYSALCQEASIVPIVEPEVLLDGDHTIERCYEATEATLSAVFAALRAHNVSLEHTV
                     LKASMVVSGKDCPKQAGIERVAEETVRCLKRTVPAALPGVVFLSGGQSDEAATAHLNE
                     MARMPGLPWPLTFSYSRALQNPPLKAWRGQSGNVAAAQRAFYHRARMNGLAAQGKYRP
                     ELEKQAA"
     gene            1892..2791
                     /locus_tag="VEM33_01780"
     CDS             1892..2791
                     /locus_tag="VEM33_01780"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYS50588.1"
                     /translation="MPFPLYESKLKSLKLLGRGKVRDMYAVDGDRLLIITSDRLSAFD
                     VVLPDPIPDKGRVLNEMSNFWFRRLGHIVPNHLDGAEPESVVKGGDEAAQVKGRAVVV
                     RKLKPLPIEAVVRGYLIGSGWKDYQETGVVCGIALPAGLKQADKLPEVIFTPATKAEA
                     GAHDENISYEQAEKLIGKTLASKVRDVSIRLYREAAEYARARGIIIADTKFEFGTDEK
                     GNLVLIDEALTADSSRFWPADEYRPGISPPSFDKQYVRDYLEKLRWKKTPPAPRLPAE
                     VIAKTSEKYREALFRLTGRKLGE"
     gene            2827..3288
                     /gene="purE"
                     /locus_tag="VEM33_01785"
     CDS             2827..3288
                     /gene="purE"
                     /locus_tag="VEM33_01785"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYS50589.1"
                     /translation="MGSASDWEVMREAAKQLTGFSVPYEAKVLSAHRSPDALTEWVEQ
                     LTAKGASCFIAGAGGAAHLAGVIAAKTTLPVLGVPMPSKYLQGLDSLLSIVQMPKGIP
                     VATFAIGEAGAANAGLFAVAMLAQGDAKLAKLLAQFRASQAEAVKSAKLPG"
     gene            3360..>3892
                     /locus_tag="VEM33_01790"
     CDS             3360..>3892
                     /locus_tag="VEM33_01790"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYS50590.1"
                     /translation="MAILRRGGLVAFPTETVYGLGADASNPAAVARIYEVKGRPAGHP
                     VIVHIGDIGQLARWARDIPEAATKLATHFWPGPLTLVLRRAPGVGSQLSGGQDTIGLR
                     VPGHPVALQLLREFGGGIAAPSANRFGRISPTAAEHVRSDLASEVELILDGGACEIGI
                     ESTIVDLSRGRPVLLRPG"
BASE COUNT          713 a         1208 c         1308 g          663 t
ORIGIN      
        1 cgagggttgc gggcgccatc gcatccgggg aatctcgcct tggcgcgaca aactcgcgga
       61 gatgggggcg aaggaagcgg ccaaggcgat ccgcgacagc ggcctcgtgg tcacggggct
      121 ttgccgtgga gggatgtttc ccggggcgga ccgcagggcg cgtcaggccg cgatcgacga
      181 caatctgcgc gcgatcgacg atgcagcgac gctcgaagca aggtgcctgg tgctcgtcgt
      241 cggcgggctg ccgctcggct cgaaagacct agccggcgcg cgagcgatgg taagggacgg
      301 tatcggcgga gtgctcgatc acgcgcgctc ggcgggcgtc ccgctcgcga tcgagccgct
      361 gcacccgatg tactgcgccg accgggcctg cgtgaacacc ctcgcgcagg cgctcgagct
      421 ctgcgacgag ctggacggca gtgggagcgg agccctcggt gtcgtagtgg atgcctacca
      481 cgtctggtgg gacccctcgc tcaagactca gatcgaacgc gcaggcgaca agcgcctgct
      541 tgcgttccac atctgcgact ggctggtgcc caccgccgac ctgctcaccg accggggaat
      601 gatgggtgat ggcgtgatcg atcttccgct catacgtgcg tggatggagg aggcggggta
      661 ccggggtttc cacgaggtcg agatcctgtc ttcgcgaaat tggtggcggc gggacccgga
      721 tgaggtgctc gcgagctgtc gcgtgcggca tcagacagcc tgttagcatt ccaaggaggc
      781 cgccccgttt ggcggtgccg gggccgggaa ggtaaaattg ccaattccgg gtttcccttt
      841 cccatacagc aacaggaccg tctcatggac cgaaatctcc tttccaaaac cgccggggcc
      901 atggtcgcaa gaggcaaggg gctgctcgca gccgacgagt ccagcggcac atgcgagaag
      961 cgattcaagt cggtcggagt cgagtgcacg gaggccaacc gtcgcgccta ccgcgatttg
     1021 ctgttcacta ccaagggcat cgaggaattc gtaagcgggg tcatcctgtt cgacgaaacc
     1081 ctccgtcaaa gcacggtatc gggcaacgag ccgttcccga gatatctcga gaaaaagggt
     1141 gtcatccccg gcatcaaggt ggacaagggc acgcacgacc ttgcgctctc tcccggagaa
     1201 aaggtaaccg agggactgga cggcctgcgt ggtcggctcg aggactattt cgggctcggc
     1261 gcgcgcttcg ccaagtggcg cgcggtgatc acaatcggtg ccgggatccc gagccatgcg
     1321 tgcatctacg ccaacgcgca cgcgctggcg cgctactcgg ccctgtgcca ggaggcgtcc
     1381 atcgtgccca tcgtcgaacc cgaggtcctg ctcgacggcg accacacgat cgagcgctgc
     1441 tacgaggcga ccgaggcaac cctttccgcg gtattcgcgg cgctgcgcgc ccacaacgtg
     1501 tcgctcgagc ataccgttct taaggcttcg atggtcgtct cgggcaagga ctgcccgaaa
     1561 caggccggga tcgagcgcgt ggccgaggaa accgtgcgct gcctgaagcg caccgtgccc
     1621 gcggccctgc ccggtgtcgt gtttctctcg ggcggccaaa gcgatgaggc tgcaacggca
     1681 catctcaacg agatggcgag aatgccggga ctgccttggc cgctcacatt ctcctacagc
     1741 cgagcgctgc agaacccgcc tctgaaggcc tggcgcggcc agtcgggcaa cgtcgcagcc
     1801 gcccagcggg cgttctatca ccgggcccgc atgaacgggc tcgcggctca aggaaaatac
     1861 cgtccggagc tggagaaaca ggcagcatag aatgcctttc ccgctctacg agtcaaagct
     1921 caagagcctg aagctgctag gccgcggcaa ggtccgcgat atgtatgcag tggacggcga
     1981 ccgcttgctc attatcacca gcgatcggct ttcggccttc gacgtggtcc ttcccgatcc
     2041 gatacccgac aaggggcgcg tactgaacga aatgtccaat ttctggttca ggaggctcgg
     2101 acacatcgtt cccaaccatc tcgacggcgc cgagcccgag tcggtggtga agggcggcga
     2161 cgaagcggcg caggtcaagg ggcgtgcggt agttgtaaga aaactcaagc cgctgccgat
     2221 cgaggcggtg gtacgcggtt atctcatcgg ctcgggctgg aaggactatc aggagaccgg
     2281 cgtagtttgc ggcattgcgc tgcccgcggg tctgaagcag gcggacaagc tgccggaggt
     2341 gattttcacg ccggcaacca aggcagaagc cggcgcgcac gacgagaaca tatcgtacga
     2401 gcaggccgag aagttgatcg gcaagacgct tgcctcgaag gtgcgtgacg tcagtattcg
     2461 cctctaccgc gaggcggccg agtatgcgcg ggcgcgaggt atcatcatcg ccgacaccaa
     2521 gttcgagttc gggacggatg agaagggaaa tttggttctg atcgacgagg cgctgaccgc
     2581 ggactcctcg cggttctggc ccgccgacga gtatcggccc ggaatcagcc ctccttcatt
     2641 cgacaagcag tacgtgcgcg actacctgga gaagcttcgc tggaagaaga cgccccccgc
     2701 ccccaggctt cccgccgaag tgatcgcgaa gacctccgag aagtatcgcg aggccctgtt
     2761 ccgtctgacc ggacgaaaac tcggagaatg aaggtggccg gtcgctcgcc tcttgtcggg
     2821 atcgtcatgg gaagcgcaag cgattgggaa gtgatgcgcg aggcggcaaa gcagctcacc
     2881 ggtttttccg tgccgtacga agccaaggtg ctgtcggcgc accgctctcc cgacgcgctc
     2941 accgaatggg tggaacagct cactgccaaa ggcgccagct gcttcatcgc gggtgccgga
     3001 ggtgcggcgc atctggccgg tgtgatcgca gcgaaaacga cactccccgt gctcggcgtg
     3061 cccatgccct ccaagtatct gcaaggattg gattcattgc tgtcgatcgt gcagatgccc
     3121 aaggggatcc cggtggcgac ttttgccatc ggcgaggcgg gcgccgccaa cgcgggactc
     3181 tttgccgtcg cgatgcttgc ccagggagac gcgaaactcg ccaaacttct cgcgcagttc
     3241 cgcgcgagcc aggccgaagc ggtgaagagc gccaagttgc cgggttgaca cgggcttcgt
     3301 cccgcctttc cttgcctgcg aacgtcgacg cgctccaatc cgttccgatt cgggatgcag
     3361 tggcgatact gcgacgcggc ggcttggttg cctttcccac cgaaacggtc tacggactgg
     3421 gtgccgacgc gtccaatccc gcagcggtag cgcgaatcta cgaggtcaag ggccgtcccg
     3481 cggggcatcc cgtcatcgtc catatcggcg acatcgggca attggctcgc tgggcgcgcg
     3541 atatccccga ggccgcgacg aaactcgcaa cgcacttctg gccggggccg ctgaccttgg
     3601 tgctgaggcg tgcgccaggt gtcggtagtc agctcagcgg cgggcaggat acgatcggcc
     3661 tgcgcgtccc cgggcatccg gtcgccctcc agctcctccg ggaatttggc ggaggcatag
     3721 ctgccccgtc ggccaaccgg tttggccgga tcagcccgac ggcagcggag catgtgcgaa
     3781 gcgacctggc gagcgaagtc gagttgatcc tggacggagg cgcgtgcgag atcggcatcg
     3841 aatcgacgat cgtggatctc tctcgcgggc ggcccgtgct gcttcgtccc gg
//
LOCUS       DASPZD010000097         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_76999, whole genome shotgun sequence.
ACCESSION   DASPZD010000097 DASPZD010000000
VERSION     DASPZD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76999"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VEM33_01795"
     CDS             <1..692
                     /locus_tag="VEM33_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50591.1"
                     /translation="GVLQAVRSPNHAELIPAALPAHGEWVGSRLNVFVQAYNTRLVDK
                     AELPKSYAELLHPRWKGRLGIEAGDEDWFAAVVTALGEAEGLRLFRELVAANGISVRR
                     GHTLLTNLVASGEVPLALAVYNFTAEQLKRKGAPLDWFVIAPAVARANGVALARRAPH
                     PHAALLCYDFMISDEGQRILQERGFVPARRSLQGPLGAELLRIVDPALMLDHAEKWGR
                     LYESTFLSQRR"
     gene            746..1840
                     /locus_tag="VEM33_01800"
     CDS             746..1840
                     /locus_tag="VEM33_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50592.1"
                     /translation="MIIDFDSHLREGYFMDEVYKLDGPYERFRPVKLNDGRTHNTKFL
                     HSLDPISPQGHAAHRHPYIYDPKTNWRGGDVAARQVGGYDMARRREDIGKEGIDKQLI
                     FPTQITVATSNIGGLGRECARLFNDWVAKLVRGHDDVFLPVAMAPAGCPEAMANELRR
                     CVKELGFRTAHLVPYCGTRNLDDPSFYPYYQMAEEFDVPLLCHPNSNGELVDRFDNFY
                     KTHVLGRPTNCSAALVALVLGGVFEKFPKLKVVFFECSAEWILYWMHRMDDDWEWAKD
                     FPQISGMLKMAPSEYLRRNCYVTCEADEADLARPIAELGDDHILMASDYPHFDSEFPH
                     TVANIRARKDLTERQKDKILGENAARLLRL"
     gene            1847..>2500
                     /locus_tag="VEM33_01805"
     CDS             1847..>2500
                     /locus_tag="VEM33_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS50593.1"
                     /translation="MLLRFLMLLGLAVAPASFAVEPTPGVLAVASYTGADRAERLIGA
                     AKREGELMLYSSLTQDDQLKLVAAFRRRYGVTVKFWRGSQAHILQRVLSETRGGRFEF
                     DVLETNSPQIEALAREKLLQKMNSPYIEQELLPETVPAHGEWAADRLNLLVYAYNSNA
                     VKLADLPKSWQDLLDPKWRKRIGVESTNVEWFAALVKSFGEKAGLELFQRMADNGLAV
                     "
BASE COUNT          451 a          811 c          811 g          427 t
ORIGIN      
        1 aaggagtgct tcaggcggtg cgctcgccca atcatgccga gctgattcct gcggcgctgc
       61 ccgcgcacgg ggagtgggtc ggctcgcggc tcaacgtctt cgtgcaggct tacaacacgc
      121 ggctggtgga caaagcggag ctgcccaaga gctacgccga gcttttgcat ccccggtgga
      181 aaggccgcct cggcatcgag gccggcgatg aagactggtt cgccgccgtg gtgacggcgc
      241 tcggcgaggc ggaagggctg cgtcttttcc gcgagcttgt cgccgcgaac ggcatttcag
      301 tacggcgggg gcacacgctg ctcaccaact tggtcgcctc cggcgaagtg ccgcttgcgc
      361 ttgcggtgta caacttcacc gccgagcagc tcaagcgcaa aggggcgccg ctcgactggt
      421 tcgtgatcgc acccgccgtt gcgcgcgcca acggcgtcgc cctcgcgcgg cgtgccccgc
      481 atccgcatgc ggcgctcctc tgctacgact tcatgatcag cgacgagggc cagcgcattc
      541 tgcaggagcg cggtttcgtg ccggcgcgcc gctcgctgca aggtccgctc ggcgccgagc
      601 tgctcaggat cgtcgatccc gcgctcatgc tcgaccacgc cgagaaatgg ggccggctct
      661 acgaatccac cttcctttcc cagcggcgtt gacgttgctc gcgcgcgacg ctgcgcgcac
      721 aatccccgca acgccggaga ccaacatgat catcgacttc gattcccatc tgcgcgaagg
      781 ctatttcatg gacgaggtgt acaagctcga cgggccgtac gagcgcttca ggcccgtcaa
      841 gttgaacgac ggccgcacgc acaacacgaa gtttctgcat tccctcgatc ccatcagccc
      901 tcagggtcac gccgcgcacc ggcatcctta catctacgac ccgaagacaa attggcgcgg
      961 cggcgacgtc gccgcgcggc aggtgggcgg ctacgacatg gcgcgccgcc gcgaggacat
     1021 cggcaaggaa ggcatcgaca agcagctgat attcccgacc cagatcacgg tcgccacctc
     1081 gaacatcggg gggctggggc gcgagtgcgc gcgcctgttc aacgactggg tcgcgaaact
     1141 ggtgaggggt cacgatgacg tttttctgcc ggtcgcgatg gcgccggcgg ggtgtcccga
     1201 ggccatggcc aacgagttgc ggcgctgcgt gaaggagctc ggcttcagga ccgcgcacct
     1261 cgtgccctac tgcggcacgc gcaatctgga cgatccgagc ttctacccct attaccagat
     1321 ggccgaagag ttcgacgtgc cgctcttgtg ccatccgaac agcaacggcg agctggtcga
     1381 ccggttcgac aatttctaca agacgcacgt gctcgggcgc ccgaccaact gcagcgcggc
     1441 gctggtggcg ctggtgctgg gcggcgtgtt cgagaaattc ccgaagttga aggtggtgtt
     1501 cttcgaatgc agcgccgaat ggatccttta ctggatgcac cgcatggacg acgactggga
     1561 atgggccaag gacttcccgc agatttcggg catgctcaag atggcgccct cggagtacct
     1621 acgccgcaac tgttacgtaa cctgcgaggc ggacgaagcc gatctcgcgc ggccgatcgc
     1681 cgagctgggc gacgatcaca ttctcatggc gagcgactat ccgcatttcg actcggagtt
     1741 cccgcatacc gtcgcaaaca tccgcgcgcg aaaagatctc acggagcggc agaaggacaa
     1801 gattctcggc gagaacgcgg cgcgcctgct gcggctctag agccccgtgc tgcttcgctt
     1861 cctgatgctg ctcggcctgg cggttgcccc ggcaagcttc gcggtcgagc cgacgcccgg
     1921 agttctcgcg gtcgcttcct acacgggcgc ggatcgcgcc gaacgcctga tcggcgcagc
     1981 taagcgggag ggcgagctga tgctgtactc ctccctcacg caggacgacc agctcaaact
     2041 cgtcgcggcc ttcaggcgcc gctacggggt caccgtgaag ttctggcgcg gcagccaggc
     2101 gcacatcctg cagcgcgtgc tcagcgaaac gcgcgggggt cgcttcgagt tcgacgtgct
     2161 cgagaccaac tccccgcaga tcgaggcgct cgcgcgcgaa aagctgctgc aaaagatgaa
     2221 ctcaccctat atcgagcagg agctgctgcc cgaaacggtg ccggcgcatg gcgaatgggc
     2281 agcggatcgg ctcaatctgc tggtgtacgc ctataacagc aacgcggtga agctcgccga
     2341 cctgccgaaa tcctggcagg acctgctcga cccgaaatgg agaaagcgca tcggcgtgga
     2401 atccaccaac gttgaatggt tcgccgctct ggtgaagtcg ttcggcgaga aagcggggct
     2461 cgagctgttt cagcgcatgg ccgacaacgg gctggcggtg
//
LOCUS       DASPZD010000098         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1438958, whole genome shotgun sequence.
ACCESSION   DASPZD010000098 DASPZD010000000
VERSION     DASPZD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438958"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1226)
                     /locus_tag="VEM33_01810"
     CDS             complement(57..1226)
                     /locus_tag="VEM33_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYS50594.1"
                     /translation="MTVQIKLPSAGGNLEEFRLSAPRDFGTVGRKAFNRIALAAAHVV
                     ADPLARGNPWLDAAVDWEATIAYRRYLWSLGLGVAEGMDTAQRGMGLDWKSSLELIHR
                     SIDASGDIEGAVLFSGAGTDQLVPSGGVTLDEVVAAYEEQCESIESLGGRIILMASRA
                     LAACARSSDDYQRVYGRVLSQTRRPVIIHWLGEMFDPQLTGYWGEESHGKAMNVALAV
                     IEANRAKVEGIKISLLDRQKEIDMRRRLPRGVRMYTGDDYNYPDLIEGDRTGHSDALL
                     GIFDAIAPAASAALGRLASGDAPGFHDILAPTVPLARHIFAAPTRFYKTGLVFLAWLN
                     GHQEHFVMVGGQQSARSVLDLAQTFRLADAAGLLRDPELAASRMGRFLAVNGVGG"
     gene            complement(1252..2403)
                     /locus_tag="VEM33_01815"
     CDS             complement(1252..2403)
                     /locus_tag="VEM33_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYS50595.1"
                     /translation="MSEQRLGVIMNGVTGRMGTNQHLARSVMAIRREGGVRLADGTRV
                     IPDPILVGRNPEKLKKLAAEHGVGRWTTDLDEALANPDDAVFFDAASTKLRPAVLKKA
                     LAARKHIYCEKPVATSLTLALDLYRAAKKAGVRHGVVQDKLWLPGLLKIKALRDAGFF
                     GKILSVRAEFGYWVFEGDEQPAQRPSWNYRKKDGGGIILDMLCHWRYVLDNLFGEVKS
                     VSCLGARHIATRWDENGRKYGATADDAAYATFRLAGGVIAQFNSSWCVRVRRDDLAIF
                     QVDGTKGSAVAGLTRCFAQSLAETPRPVWNPDVRQTHDFGTDWKEVPDDKPYDNAFKA
                     EWELFIRHVCEGEPFRWNLLEGAKGLQLVDCAVKSWRSQRWVDIPRLKA"
     gene            complement(2419..>3415)
                     /gene="pyk"
                     /locus_tag="VEM33_01820"
     CDS             complement(2419..>3415)
                     /gene="pyk"
                     /locus_tag="VEM33_01820"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYS50596.1"
                     /translation="GVLSNNKGINRLGGGLTAPALPPKDMEDIRTAAKIKVDYLAVSF
                     PKSSADMVMARQLLRAAGGEAFLIAKIERAEAVEPKALIDIMSACEGIMVARGDLAVE
                     VGDASVPALQKRMIRLAREHNKLTITATQMMESMISSPVPTRAEVSDVANAVLDGTDA
                     VMLSAETASGKYPVETIASMSRICAVAEDSAEVTLDRDFLDRVFTRVDQSIAMAALFT
                     AYHLKVKAIAALTESGSTALWMSRINCGVPIYALTSQTVTRYRCALFRDTYPLMVKYV
                     GNDREELLEEAENVLVKSGVVQKGDLIVLTIGEPIGKAGGTNTMKIVRVGEHRKG"
BASE COUNT          601 a         1101 c         1048 g          665 t
ORIGIN      
        1 cctttcccgc accgtggcgg tattgagtga aagcagctcg gggtcgggcg gattgctcat
       61 ccaccgacgc cgttcacggc gagaaaacgc cccatcctgg acgcggcgag ctcgggatcg
      121 cgcagcaatc cggcagcgtc cgccagccgg aaggtctgag cgagatccag caccgaccgg
      181 gccgattgct gaccgccgac catgacgaag tgctcctggt ggccgttcag ccaggcgagg
      241 aacacgagac cggtcttgta gaagcgagtc ggcgcggcaa agatgtgacg cgcgagcggt
      301 accgtcggcg cgaggatatc gtgaaagccg ggcgcgtcgc ccgacgccaa gcgacccaag
      361 gcggcggagg cggccggggc aattgcgtcg aaaatgccga gcagcgcgtc ggagtggcct
      421 gtccggtcgc cttcgatcag gtcggggtag ttgtagtcgt ccccggtgta catgcgcact
      481 ccccgcggaa ggcgcctgcg catgtcgatt tccttttgcc gatcgagcag cgagatcttg
      541 atgccctcga ctttggcacg gtttgcctcg atcacggcga gtgcaacgtt catcgccttg
      601 ccgtgacttt cctcgcccca gtagccggtg agttgcggat cgaacatctc gccgagccaa
      661 tggatgataa cgggccgcct tgtctgcgag aggacgcgcc catagactcg ttgataatcg
      721 tccgacgagc gtgcgcacgc ggcaagcgcg cggctcgcca tgaggatgat ccgcccgccg
      781 agactttcga tcgattcgca ttgttcttcg tacgctgcga cgacctcatc gagtgtgacg
      841 ccgccggagg gcacgagttg atcggtgccc gcgccgctga agagcaccgc gccttcgatg
      901 tctcctgacg catcgatcga gcgatggatg agctcaagcg aactcttcca gtcaagaccc
      961 atgccgcgct gcgccgtgtc catcccttcg gccacgccca agccgagcga ccacaggtag
     1021 cgccggtagg caatagtcgc ttcccagtcg actgcggcat cgagccacgg attccctcgg
     1081 gcgagaggat cggcgaccac gtgcgccgcg gcgagcgcga tgcgattgaa cgccttcctt
     1141 ccgaccgtgc cgaaatcgcg gggcgcgctc agccggaact cttcgagatt cccgcctgcg
     1201 gaaggcagct tgatctgcac cgtcagcttt tcaggggcat tcatgcccgg tctacgcttt
     1261 cagcctggga atgtcgaccc agcgttgcga tcgccacgac ttcacggcgc aatcgaccag
     1321 ttgcagcccc ttcgcacctt ccagcaggtt ccacctgaag ggctcgcctt cgcatacgtg
     1381 gcggatgaaa agctcccatt cggccttgaa cgcgttatcg taaggcttgt cgtccggaac
     1441 ctctttccag tccgtaccaa aatcatgtgt ctgacgcacg tccgggttcc ataccggccg
     1501 aggcgtttcg gcaagggact gggcaaaaca gcgtgtgagc ccggccacgg cagagccctt
     1561 cgttccgtcc acctgaaaaa ttgccaagtc atcgcggcgc actcgcacgc accacgagct
     1621 attgaactgg gcgatgaccc cgcccgcgag ccgaaaggtt gcgtacgcgg cgtcgtccgc
     1681 ggtcgccccg tatttgcggc cattctcgtc ccagcgggtc gcgatgtgcc gcgctcctag
     1741 acaggaaacg ctttttactt ctccgaaaag gttgtcgagc acgtaacgcc agtggcacag
     1801 catgtcgagg ataattccgc cgccgtcctt cttgcggtaa ttccaggagg gccgctgtgc
     1861 gggctgctca tcgccctcga atacccaata gccgaattcg gcccggaccg agaggatttt
     1921 cccgaagaaa ccggcatcac gcagtgcctt gatcttcagc agccccggca gccagagctt
     1981 gtcctggacg acaccgtgcc gaactcccgc tttcttggct gcccgataca ggtcgagtgc
     2041 tagcgtgagc gaggtcgcga cgggtttctc gcagtagatg tgtttcctcg cggcgagggc
     2101 tttcttgagc acggcgggcc ggagctttgt cgaagcggcg tcaaaaaaaa ccgcgtcgtc
     2161 gggatttgcg agcgcctcgt ccaggtcggt cgtccagcga ccgacaccgt gctcggcggc
     2221 gagcttcttc agcttctcag ggttgcgtcc aaccaggatc gggtcgggga tcacccgcgt
     2281 gccgtcggct agccgcaccc caccctccct gcggatagcc atgacggagc gcgccaggtg
     2341 ctggttcgtg cccatgcggc ccgtcactcc attcataatg acgccgagtc tttgctcaga
     2401 catggcttct cttcgagatt atcccttccg gtgctccccg acgcggacga tcttcatcgt
     2461 gttggtcccc ccggccttgc cgatcggctc gcctatggtg agcacgatga ggtctccctt
     2521 ctgcaccacg ccgctcttca ccaagacgtt ttcggcctcc tccagcagct cctcgcggtc
     2581 gttgccgacg tacttcacca tcaggggata ggtgtcgcgg aacaacgcgc aacggtaacg
     2641 cgtcacggtc tgcgaggtga gcgcgtagat cggaacgccg cagttgatgc gcgacatcca
     2701 caacgcagtc gaccccgatt cggtgagcgc ggcgatcgct ttcactttga ggtgataggc
     2761 ggtgaatagc gcggccatgg cgatcgactg atccactcgc gtaaagacgc ggtcgagaaa
     2821 gtcgcggtcg agggtcacct cggccgagtc ctccgcaact gcgcagatgc ggctcatcga
     2881 ggcgatggtc tcgaccgggt acttgccgct tgcggtttcc gcggacaaca tcaccgcatc
     2941 ggtcccatcg aggacggcat tggcgacgtc cgacacctcg gcgcgggtgg gtaccgggct
     3001 cgaaatcatc gattccatca tctgcgtggc ggttatggtg agcttgttgt gctcgcgcgc
     3061 aagccggatc attcgttttt gcagcgccgg gaccgaggca tccccgacct cgaccgccag
     3121 atccccgcgc gctaccatga tgccctcgca ggccgacatg atgtcgatga gcgctttcgg
     3181 ctcaacggct tctgctcgct cgatcttggc tatgaggaac gcctcgccgc ccgcggcgcg
     3241 caatagctgc cttgccatga ccatatcggc gctggacttg gggaaagaaa cggcgaggta
     3301 atccaccttg attttcgccg ctgttcgaat gtcttccatg tccttcggcg gaagcgcggg
     3361 ggcggtgagg ccgcctccga ggcgattgat gcccttgttg ttggaaagca ctccc
//
LOCUS       DASPZD010000099         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1159840, whole genome shotgun sequence.
ACCESSION   DASPZD010000099 DASPZD010000000
VERSION     DASPZD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159840"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VEM33_01825"
     CDS             <1..716
                     /locus_tag="VEM33_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS50597.1"
                     /translation="DEEATLGETISQGSDHVEVNGVKKHVIGYLGDFLFPPERVRAPV
                     KSLSGGERNRLLLARLFSRPANVLVLDEPTNDLDIETLELLESLLQEYSGTLFLVSHD
                     REFLDNVVSQTIASEGGGEWKEYAGGYSDWQRVRSRPAAPETAKRKTAALQPRRHTQS
                     NKLGYKEERELAELPDKLKALEREQEQIAGRLADPALYRDRSGEIKPLQTRHAAIEKE
                     LTRALTRWEELEGKKRGAG"
     gene            complement(750..1037)
                     /locus_tag="VEM33_01830"
     CDS             complement(750..1037)
                     /locus_tag="VEM33_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50598.1"
                     /translation="MRKFASTTLKLPGRLKQRIAPLARSSGKTPHAWMVEALETHTAL
                     AEKRRTFVADALAAEKEVEHAGRAYPAGDVHRYLHARVVGRKAKRPKPARW"
     gene            complement(1111..>2204)
                     /locus_tag="VEM33_01835"
     CDS             complement(1111..>2204)
                     /locus_tag="VEM33_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYS50599.1"
                     /translation="CGTQSQTMAVAAVAKALGIPVEKVKLNLNLLGGGFGRRGHRDEE
                     FVVDSVLLSKEVKRPVKVLWTREDDVHNGRFRPLSVHFLRAGLDASGRIVAWQHRVAC
                     DEITAFQDPLRYKGGGEKDFLAMAGSELRTYDIPNRLAEQLPQETGIRTSSLRGIGFG
                     PNKFATEAFLDEVAAKHGLDPIEVRLQLLKNTPRGQAVIGAAVEMSDYRRKRPGRGLG
                     FSFIDYAGTMVAAVAEVSVDKARGKITVHDFWLALDPGIAVQPDNVIAQTESSIVYGL
                     GLALTERITIKDGAVQQSNILDYGVPRMHDIPELHIRLMSTPNRPTGAGQMATPIVAP
                     VIASAVFAANGARVRHTPFLPERVPAAMA"
BASE COUNT          395 a          721 c          734 g          354 t
ORIGIN      
        1 tcgatgaaga ggcaacgctc ggcgaaacca tcagccaggg aagcgatcat gtcgaagtga
       61 acggcgtgaa aaagcatgtg atcggttatc tcggggattt tctcttcccg cccgagcgcg
      121 tccgggcgcc cgtgaaatcg ctctccggcg gcgagcgcaa ccgcctgctg ctcgcgcgcc
      181 tcttcagccg gcccgccaac gtgctggtgc tggacgagcc gaccaacgat ctggacatcg
      241 aaacgctcga gctgctggag tcgctgctgc aggaatattc gggcacgctg ttcctcgtga
      301 gccacgaccg cgaatttctc gacaacgtcg tgagccagac catcgcgagc gaaggcgggg
      361 gcgaatggaa ggaatacgcc ggcggctaca gcgactggca gcgcgtgcgg tcgagacccg
      421 cagctccgga gaccgcgaag cggaagaccg cggcacttca accgcgtcgg cacacacaaa
      481 gcaacaagct cggctacaag gaagagcgcg agctcgccga attgccggac aagctaaagg
      541 cgctcgaacg ggagcaagag caaatcgccg gccgtctcgc ggatcccgcg ctgtatcgtg
      601 accggtcggg cgagatcaag ccgctgcaga cgcggcatgc cgcgatcgag aaagagttga
      661 cgcgggcgct tacgcggtgg gaagagctgg aggggaagaa acggggggca ggttgaaacg
      721 gccggccgaa cttcaacgct cctctggcgt taccaccgcg ccggtttcgg acgcttcgct
      781 ttccttccga cgacgcgagc gtggaggtaa cggtgcacgt cgccggccgg gtaggccctg
      841 ccggcatgct cgacctcttt ctccgcggcg agcgcgtcgg cgacaaaagt cctgcgcttt
      901 tcggcgagag cggtatgcgt ttcgagcgcc tccaccatcc aggcgtgcgg cgtcttgccc
      961 gaggacctcg ccaggggggc gatgcgctgt ttgagtcgcc cgggaagctt cagcgtcgtg
     1021 gaagcgaact tgcgcatgaa gaagtaatag aggagctgga ggggaagacc tcgggcagaa
     1081 tcctcccctg cgcgcgttca aacccagcgt tcacgccatc gcggcgggca cgcgctcggg
     1141 caggaaaggt gtgtggcgca cgcgcgcgcc gttcgccgcg aagacggcgc tcgcgatgac
     1201 gggtgccacg atgggcgtcg ccatttgccc tgcgcccgtc ggacggttcg gcgtggacat
     1261 cagcctaata tgcagctcgg gaatgtcgtg catgcgcggc acgccgtagt ccagaatgtt
     1321 cgactgctgc accgcgccgt ccttgatggt gatacgctcg gtgagcgcga gtcccagccc
     1381 atagacgatc gagctctcgg tctgcgcgat tacgttgtcg ggctgcaccg cgatgccagg
     1441 atcgagcgcg agccagaaat cgtggacggt gatcttgcca cgcgccttgt ccaccgacac
     1501 ctcggccacc gccgcgacca tcgtccccgc gtagtcgatg aacgagaatc cgaggcctcg
     1561 tccgggccgc ttgcggcggt agtcggacat ctcgaccgcg gcgccgatca ccgcctggcc
     1621 gcgcggcgtg ttcttcagaa gctgcagccg cacctcgatc ggatcgaggc cgtgcttcgc
     1681 cgcgacctcg tcgaggaagg cctcggtcgc gaacttgttg ggcccgaagc cgatgccgcg
     1741 cagtgacgaa gtgcggatgc cggtttcctg cggcagctgc tccgcgaggc ggttcgggat
     1801 atcgtaggtg cgcagctccg agcccgccat cgcgaggaaa tccttctccc cgcccccctt
     1861 gtagcgcagc gggtcctgga acgcggtgat ttcgtcgcag gcgacgcgat gctgccatgc
     1921 gacgatgcgg cccgaggcgt cgagccccgc gcgcaggaaa tgcaccgaga gcgggcggaa
     1981 gcggccgttg tgcacgtcat cctcgcgcgt ccacagcact ttcaccggcc gcttcacctc
     2041 cttcgagagc agcaccgagt cgacgacgaa ctcctcgtcg cgatgcccgc gccgcccgaa
     2101 cccgccgccg agcaggttca aattcaattt caccttctcc accgggatgc cgagcgcctt
     2161 ggcgaccgcc gccaccgcca tggtctgcga ctgggtgccg cacc
//
LOCUS       DASPZD010000100         5674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_808515, whole genome shotgun sequence.
ACCESSION   DASPZD010000100 DASPZD010000000
VERSION     DASPZD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808515"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..435)
                     /locus_tag="VEM33_01840"
     CDS             complement(175..435)
                     /locus_tag="VEM33_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYS50600.1"
                     /translation="MFGNLGFPELLIIMVVILLLFGAKRIPEIAGSMGKGIREFKKNI
                     NDATREVTSETSAAEPPRLTSAELERRRATEEEERPAPKRLM"
     gene            complement(731..1639)
                     /locus_tag="VEM33_01845"
     CDS             complement(731..1639)
                     /locus_tag="VEM33_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYS50601.1"
                     /translation="MGAIVSSELPLVGQVSAHLLAMRGKMFRPTLLLLSSEAADEPRD
                     EAITLAASLELIHLATLVHDDAVDHSVLRRGMPTVNALFSHQISVIMGDFLYSTALTH
                     LVGLGNLEALQALTRASTEMTLGEMRQLAVTDPLRFSEREYYDLIRSKTASLMRTACE
                     LGALAGARRYRDALGAFGENLGMAFQIADDLLDYREEEETTGKPSGLDLREHKVTLPL
                     IHALREMPASSRKEVERLFDSETPSDADIASVVRIVEENGGFDYARQQEEEYAERAHE
                     ALVDLPDTVARRSLTASISYVMERHS"
     gene            complement(1789..4047)
                     /locus_tag="VEM33_01850"
     CDS             complement(1789..4047)
                     /locus_tag="VEM33_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50602.1"
                     /translation="MSNVEKLKKKAAEFEQKKQFDRALEVYLQIIGQTEGQEDRDVTV
                     YNRVGDLYLRLNKTDQAINYYEQAVDLYTDGGYLNNAIALCNKILRHAPSRHQIYYKL
                     GKISAKKGFNSDAKKNFLEYADRMHRAGKDTDAFKALQEFADLCPGQDDIRLMLADQL
                     AKAGKTAEAIEQLQILHESLDAEGRTTEAEATVQRIHSLDPSVEPRRSKTPVKRDAGG
                     LVFLDVGGSPPKPRPKVEGRTAAVPGAPPPRPKTPPHVQPAPEFEALPDIEPSPVESI
                     PGLEIGSTSFAGLETPSSVDNIEPAFDLISDTDVAPLEGLESAADMSQFDIDPEMGGN
                     ALGVEGLISSEPIEHTPESGEPGLGQPLATEDPLAGLNILEDDRFFIHEPVTEDNSAN
                     AASEETAATARADHTAPAVEDQVPPIEETFDLQLEHPELHPEPEPEPEPPKPAPRPVA
                     PPPVSRAAPPAPKPVARRAPEPVAEEEPEEGVEEEQEEQPAPALSRPKLVPPLRMTKP
                     KPKKPKTPEREEPPAAPAQKQPVNPFMRQRQKPKAPSPKAGQRVSTPPSEVPRAATPK
                     ENTPQQPVDNFVDLADWLREERGPRSYRMVAEEDHNREEGEEADFTDMLEKFKAGVAA
                     NVDDEDFDSHYDLGVAYKEMGLIEEAIAEFQKALRGATQRIRAYEALGQCFIDRNDFD
                     TAITVLGRALREPGMEDEDLIGVLYLLGFASEGSKKARDAAAYYQRVFAIDIDFRDVS
                     KRLKQMTKAASK"
     gene            complement(4070..5380)
                     /gene="hutI"
                     /locus_tag="VEM33_01855"
     CDS             complement(4070..5380)
                     /gene="hutI"
                     /locus_tag="VEM33_01855"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HYS50603.1"
                     /translation="MNKDLLFVNASQIVTCAGPARARRGKEMRDAGILKNTAVAVSDG
                     RIAMMGAARDLENAFSNAEIIDCRGGVLTPGLVDSHTHAVFGRARFEEQEMRAEGIDY
                     MEIAARGGGIHASVRDFRQRTEDELYELAVDRVRDLASYGTTTLEIKSGYGLSVDDEL
                     KALRVIGRLAERQPVRIVGTWMGAHEIPPDYRTSADRRKEFIDLLVNEMLPKVAQQGI
                     ARFSDVFCEPGVFTIDETRKILEASRKAGLGLKLHADELKPAGGAELAAELEATSADH
                     LGAISEKGIAALARSDTVATLLPGTMLFLGKAKQAPARALIDAGAAVALATDFNPGTS
                     PTVNFPLMLTLGVSQLRMSLAESLNAATVNGAAALGLAGEIGQIAPGFTADLALFAIG
                     DVRELPYWYGARLCVGSWKAGAPCHPYENLGKLNPRPPEVVPTG"
BASE COUNT          969 a         1767 c         1783 g         1155 t
ORIGIN      
        1 accggcggaa gcaagttgag gcgtcggccc ggacatcgac ggcgcaatag cgggaggcgg
       61 gaggcgggaa gcgggaagcg ggaagcggga agcggaagaa gcgtaccgag atgatctccc
      121 gcactaggcg gtcctagatc agcaattccc tcgtccctcg tcccgcctcc cgccttacat
      181 cagcctcttc ggcgccggtc tctcttcctc ttccgtcgcg cggcgtctct cgagctccgc
      241 ggacgtcaac cgcggcggct ccgccgcgct cgtctccgac gtcacttcgc gcgtcgcgtc
      301 gttgatgttc ttcttgaatt ccctgattcc cttccccata gatccggcga tctcggggat
      361 ccgcttcgcg ccgaacagaa gcaggattac taccatgatg atcaacagct cggggaaacc
      421 cagattgccg aacatgtaag ctcctagaaa agcgaacggg ctatcagata agccaccact
      481 acccccagca gactcaaaag tgacacgtcg agcgcgacag ggccaagggc gaacttgagt
      541 ataatcaaat caacaggcag cggcccgatg gcgggagtaa caccggtcgt aaagaattct
      601 ttcgcagctc ccggaggcag gaaccgcctt cccacctgtg tcagaaggcc tccgatcacg
      661 aatcccgagg ccagtaccag agtgtgaaac atggaaccgc gcctgctatg accacgcggc
      721 ggtggcggca ttatgagtgt ctctccatga cgtaggaaat tgatgcggtc agtgaccgcc
      781 gcgctacggt gtccggcaaa tcaaccagcg cttcgtgggc acgctcggcg tactcttctt
      841 cctgctggcg agcgtagtcg aatccaccgt tctcttcgac gatcctgacc accgacgcga
      901 tgtccgcatc gctgggggtc tcgctgtcga acagccgctc gacttctttt cgtgaggatg
      961 ccggcatctc acgcaacgcg tggatgagag gcagcgtgac cttgtgctcc cgcagatcga
     1021 gacccgacgg cttgcccgtc gtctcctcct cttcgcgata gtcgagcaga tcgtcagcga
     1081 tctgaaacgc catccccaga ttctcgccga acgcgccgag cgcatcgcga tagcgacgcg
     1141 cgccggcaag agcacccagc tcgcaggcgg tgcgcatcag cgacgccgtc ttggagcgaa
     1201 ttagatcgta gtactcgcgc tccgagaagc gcaggggatc ggttaccgcg agctgccgca
     1261 tctcgccgag cgtcatctcc gtcgacgcgc gcgtcagcgc ctgcagcgcc tcgagatttc
     1321 ccaaaccgac caggtgcgtc agtgcggtcg agtaaagaaa atctcccatg atcaccgaga
     1381 tctggtgact gaacagcgcg ttcaccgtcg gcatgccgcg ccgcaacacc gaatgatcga
     1441 ccgcgtcgtc gtggaccagc gtcgccaggt ggatcagctc cagactcgcc gccagcgtga
     1501 tcgcctcgtc gcgcggctcg tcggcggctt cactcgacag gagcagtagc gttggcctga
     1561 acatcttgcc gcgcatcgcg agcagatgag cgctcacctg accgacgagg ggaagctcgc
     1621 tcgatacgat cgcccccatc gcctgcgaga ccctatcgag cttgtcgcgg accggaaact
     1681 ggatcgcctg gagtgtcgct ccacgcggcc cagtgacctt cacttcgggt gtgtgttcag
     1741 cggcggccgt cggctttgtc agtgtcggaa agggttgtgc tggggagctc acttggatgc
     1801 ggctttggtc atctgcttca ggcgcttgct cacatcacgg aagtcgatat cgatcgcgaa
     1861 cacgcgctga taatacgcag ccgcgtccct cgccttcttg ctgccctcgg acgcgaaccc
     1921 aagcaggtag agcacgccga tgagatcctc gtcttccatt cccggctcac gcagcgcgcg
     1981 cccaaggacc gtgatggcag tatcgaaatc gttgcgatcg atgaagcact gtccaagcgc
     2041 ttcgtacgcg cgaatccttt gtgtcgcgcc gcgcaaagcc ttctggaatt cggcaatcgc
     2101 ttcctcgatg agccccatct ccttgtaggc aacgccgagg tcgtaatggc tgtcgaaatc
     2161 ctcgtcatca acgttggcag ccacaccagc cttgaatttc tcgagcatgt cggtgaagtc
     2221 cgcctcttca ccttcctcgc ggttgtgatc ttcctcggca accatccgat atgaacgcgg
     2281 cccgcgttcc tcgcggagcc aatcggcgag atcgacgaaa ttgtcgacgg gttgctgggg
     2341 cgtgttctcc ttcggtgttg cggcgcgcgg aacctctgac ggaggtgttg agacacgctg
     2401 tcctgccttg ggcgacggcg ctttcggttt ctgacgctgg cgcatgaacg gattcaccgg
     2461 ctgcttttgc gccggcgcgg cgggcggctc ctcgcgctcg ggcgtcttcg gcttctttgg
     2521 cttgggcttc gtcattcgga gcggaggaac gagcttcggt ctgctcagcg cgggcgctgg
     2581 ctgctcctct tgctcttcct cgaccccctc ctccggttct tcttctgcaa ccggctcagg
     2641 tgcgcggcga gcgacaggct tgggcgctgg cggcgcggcc ctgctgaccg gcggcggcgc
     2701 aaccggccgg ggcgcaggct tggggggctc gggttcgggc tccggctccg ggtgcagctc
     2761 cggatgctcg agctgaagat cgaaagtctc ctcgatcggc ggtacttggt cctccactgc
     2821 cggcgccgtg tgatcggcgc gtgccgtagc cgcggtctct tcgctcgctg cgttggcgga
     2881 gttgtcttcg gtgaccggct cgtggatgaa aaaacgatcg tcctcgagga tgttgagtcc
     2941 agccaacgga tcttcagtgg ctagcggctg gccaagtccg ggttccccgg attccggagt
     3001 gtgctcgatc ggttccgaag aaatcagtcc ttccaccccg agcgcgttac cgcccatttc
     3061 agggtcgata tcgaactgag acatgtcggc ggcggactcc agcccttcga gtggcgcaac
     3121 atcggtatcg ctaatgagat cgaatgcggg ctcgatgttg tcgacggaag atggggtctc
     3181 gagaccggcg aacgatgtcg atccgatctc gagcccggga atggattcca caggcgacgg
     3241 ctcgatatcg ggcagcgcct cgaactccgg ggctggctga acgtgcggag gtgttttcgg
     3301 gcgcgggggt ggcgcgccgg gcacagcggc ggtccgaccc tcgactttcg gtcgcggctt
     3361 ggggggtgat cctccaacat cgaggaaaac aaggccgccc gcgtccctct tgactggggt
     3421 cttggagcgg cgcggctcga cgctcggatc gaggctgtgg atgcgctgca ccgtcgcttc
     3481 cgcctcggtg gtgcggcctt cggcgtcgag ggattcgtgc aggatctgca gttgctcgat
     3541 cgcttctgcc gtcttcccgg ctttggcgag ctggtcggcc agcatcaacc gaatgtcgtc
     3601 ctgtcccgga cacaagtcgg cgaattcctg gagcgccttg aacgcgtcgg tatccttgcc
     3661 ggcgcggtgc attcgatcgg cgtactcgag gaagtttttc ttggcgtcgc tgttaaagcc
     3721 cttctttgcc gagatcttac cgagcttgta gtagatctgg tggcgggacg gcgcgtggcg
     3781 gagaattttg ttgcagaggg cgatggcgtt gttgaggtaa ccgccgtcgg tgtagaggtc
     3841 gaccgcctgc tcgtagtagt tgatggcctg gtcggtcttg ttgagccgca aatagaggtc
     3901 gccgacacgg ttataaaccg tgacgtcgcg atcctcctgc ccctccgtct gtccgatgat
     3961 ctggagatag acctcgagcg cccgatcgaa ctgcttcttc tgctcgaatt cggcagcctt
     4021 ttttttcagc ttctcgacgt tggacatgta gggcggccgg gcaattgaat tacccggtag
     4081 gcactacctc cgggggacga gggttcaact tacctaggtt ttcgtagggg tgacaaggcg
     4141 cgccagcctt ccacgacccg acacaaagac gtgcgccgta ccagtatggc aactcccgta
     4201 cgtcgcctat ggcaaacagc gccaaatcag ccgtaaaacc gggcgcaatc tgcccgattt
     4261 ccccagcaag accgagggcg gcagctccgt tcacggtcgc ggcattgagc gactccgcga
     4321 ggctcatccg tagctgactc acgcccaacg tcagcatcaa aggaaagtta accgttggcg
     4381 acgtccctgg attgaagtcg gttgccagtg cgacggcggc gcctgcgtcg atgagcgcgc
     4441 gggcgggcgc ctgtttcgcc ttgccgagga aaagcatagt gccaggaagg agcgtggcga
     4501 ctgtgtctga tcgggcgagc gcggcgattc ccttctcgga gatggcgccg agatggtccg
     4561 cggaggttgc ctcgagctcc gcggcgagct cggcgccgcc ggcgggtttc agctcgtcgg
     4621 cgtggagctt gagaccgagg cccgctttgc gagacgcctc gagaattttg cgcgtctcgt
     4681 cgatggtgaa aacgccgggc tcgcagaaca catcgctgaa tcgcgcaatt ccctgttgcg
     4741 cgaccttcgg cagcatctcg ttgaccagca ggtcgatgaa ctctttgcgt cggtcggcac
     4801 tggtgcgata gtcgggcggg atttcgtgcg cgcccatcca tgtacctacg attcgcacgg
     4861 gttgccgctc ggcgagccgg ccgatcacgc gcagcgcctt cagctcatcg tcgacggaga
     4921 ggccgtagcc gctcttgatc tccagcgtcg tcgttccata cgatgcgaga tcgcgcacac
     4981 gatcgaccgc aagctcgtac agctcgtctt cagtgcgttg gcgaaagtcg cgcaccgagg
     5041 cgtggatgcc gcctccgcgc gcagcgatct ccatgtagtc gatgccttcg gcgcgcatct
     5101 cctgctcctc gaagcgagcg cgcccgaata cagcgtgagt gtgcgagtcc accaatccgg
     5161 gcgtgagcac gccgccgcgg caatcaatga tttcggcgtt cgaaaacgcg ttctcgagat
     5221 cgcgcgcggc gcccatcatc gcgatgcggc cgtcgctcac cgcgacggcg gtgttcttga
     5281 gaatcccggc gtcgcgcatc tctttgcctc tgcgcgcccg cgcggggccg gcgcaggtga
     5341 ctatctgaga cgcgtttacg aaaagaagat ccttgttcat cgcggcggtt ttggcgttcg
     5401 atcgcgtttg tgattcaatg tgtttagtga agactaccag ctaggagttg tgggcggagc
     5461 ggctggcggc cgcgagctcg ctagaggcta tgggcggaac gacgttcgcg tgttttcgcc
     5521 gctgcttgcc tctagcgagc tcgtagctcc tagccgctcc gcccacagct cctggctggt
     5581 agtcttcagt tgcagttaac cgttggattg ggcgatgcca taacatcgcg caccctcgag
     5641 aagcgcagcg gcgcctgcgc ctccccccga cacg
//
LOCUS       DASPZD010000101         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_437303, whole genome shotgun sequence.
ACCESSION   DASPZD010000101 DASPZD010000000
VERSION     DASPZD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437303"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VEM33_01860"
     CDS             <1..568
                     /locus_tag="VEM33_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50604.1"
                     /translation="GIGERAGNTSLEEIVMLLRVRQSRLGLECGVEPTEITRTSRLVA
                     ELTGTPVQPNKAVVGANAFAHASGIHQDGVLKDRLNYEIMKPEDVGLADSRIVLTARS
                     GRHAFHHRLNRLGIALAEGKDDEAWQRFLQLADTKKEVTDEDLRKIAAMSDSNGTSGH
                     EGNGHPPVTDQLHDHNHVADTLRHMIFG"
     gene            565..1404
                     /locus_tag="VEM33_01865"
     CDS             565..1404
                     /locus_tag="VEM33_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HYS50605.1"
                     /translation="MKIAYQGEPGAYSDEAVSALFPGAEAVGFPTFRLTFDALAMGAV
                     DAAVLPVENSSAGVVQEVSDLLWELPGLRVVREHVQPVRHCLLGWPGPVERALSHPQA
                     LAQCDRYLHSRQIRSVAFHDTAGAARAVAEQRQPGTAAIASKAAAARYGLYVLAESIQ
                     DDSENRTRFVVVEKGDPARPRDAGQGPREADSKLKGSIAFVTAHRPGSLAHALDCFAQ
                     RNVNLTRLDSRPILGRPFEYRFYLDFSINGEVSAAAAAEAALSALEEASAEIKLFGTY
                     PAS"
     gene            1698..3080
                     /locus_tag="VEM33_01870"
     CDS             1698..3080
                     /locus_tag="VEM33_01870"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50606.1"
                     /translation="MASGRPDAPVATDAQAKRRRLPGINLRPGSVKQARVESGFSLAQ
                     LGKGHVTAPAIYLIETGRTRPSLPTLEHIARRTGKPVEFFLAEPVGAADDASARLIEL
                     EAMVADGRNQDAIALGSSLIERGTSAFRLGRIRFFLAQAYQASSQAERASALLAEARA
                     HFEAVNDGVMLAECIGAQASLANSLQSKDALALAEQALSVCRALKPVPAPTEARLLGI
                     LANALVADREWDRAVAAYEQAIEVGGSVFDLRRVARMYSALGLAYHQAGEVEPAARYS
                     MRSVALLEVLRDRVALARAENSLAMVLMARGDFAGARGHLDRSLELCDETDLESGRSR
                     VLLSLSELSMQQGNAADARDGAQHALELAERFEEGADIAEAHVLLGRIADVGGEDESA
                     DHEFEQAIRGFEALGLHERLLQCHGIYAEILERRGELAKAYVHMKEALQATRPGLLRR
                     EQEEERVSSA"
BASE COUNT          550 a         1078 c         1137 g          543 t
ORIGIN      
        1 cggcatcggg gagcgtgccg gcaacacctc cctggaagag atcgtgatgc ttctccgcgt
       61 caggcagtcg aggctcggcc tcgagtgcgg cgtcgagccg actgagatca cgcgcacgtc
      121 acggttggtc gcggagctaa ccgggacccc ggtgcagccg aacaaggcgg tggtcggcgc
      181 caacgcgttt gcgcacgcgt ccggcattca ccaggacggc gttctgaagg accgcttgaa
      241 ctacgagatc atgaagccgg aggacgtggg tctcgccgac tcacgcatcg tgctcaccgc
      301 gcgttcgggc cgccacgcgt tccaccaccg gctcaaccgg ctcggcatcg cgctggctga
      361 gggcaaagat gacgaggcgt ggcagcgttt cctgcagctg gccgacacga agaaggaagt
      421 caccgacgag gatctgcgga agatcgcagc gatgtccgat tcgaacggca cctctggtca
      481 tgaaggaaat ggacatcccc ccgtgaccga ccagctgcac gaccacaatc acgtcgcgga
      541 cacgcttcga cacatgatct tcgggtgaag atcgcctacc agggcgaacc cggggcctac
      601 tcggacgagg cggtgtcggc cttgtttccg ggtgcggagg cagtgggctt cccgaccttc
      661 cgcctgacct ttgacgcgct ggcgatgggt gccgtggacg cggcggtgct tccggtcgag
      721 aacagcagcg ccggcgtggt gcaggaggtc tccgacctgc tgtgggagct gcccgggctg
      781 cgcgtggtgc gcgagcacgt gcagcccgtt cgccactgcc tgctcggttg gccgggcccg
      841 gtggagcgcg cgctgtctca tccccaggcg ctcgcgcagt gcgaccgcta cttgcactcc
      901 cgccagatcc gatcggtggc atttcacgac actgcaggag ccgctcgcgc ggtcgcggag
      961 cagcggcagc cagggaccgc cgccatcgcc agcaaggcgg cggccgcccg ctacgggctg
     1021 tacgtgctcg ccgagtcgat ccaggacgat tcggagaatc gcacccgatt cgtagtggtg
     1081 gagaagggcg atccggcccg gccgcgcgac gcggggcaag gcccccgcga agcggactct
     1141 aaattgaagg ggtcgatcgc cttcgtcacc gcgcaccgcc cggggagcct cgcgcacgcg
     1201 ctcgactgct ttgcccagcg gaatgtcaac ctcacgcggc tggacagccg gccgatcctg
     1261 ggccggccgt ttgagtatcg gttctacctc gacttttcga tcaatgggga ggtgtccgcg
     1321 gcggcggcgg cggaagccgc gctcagcgcc ctcgaagagg cgagcgcgga gatcaagctg
     1381 ttcgggacgt atccggcctc ttagggggtt cgggactccc cacccggcgg ctgcgccgcc
     1441 gacctaccgg acaagccgga ccaggacgcg cttcccgcgc ctactgattg ccccagtgaa
     1501 tggggaggtg aaattaaaaa gcaggccgcg gcgggaagca ctctgaccgc gacctgcgta
     1561 gcgcaagttt aacttaacaa cgttaagtat gtttcggggt tagaaatttg gcttaaaggg
     1621 cgaccggcga tcctgaaacg tgcgtttcaa gagggcgtgc cgccatcccg cccggatttc
     1681 atataaagtg acgaaccgtg gctagcggcc gtccagacgc accagttgcg actgatgcgc
     1741 aggccaagag gcgccgcctt ccgggcatca acctgcggcc tggttcggtc aagcaggctc
     1801 gcgtcgaatc ggggttcagc ctcgcccagc tgggtaaggg ccacgtcacc gcaccggcga
     1861 tctacctgat cgagaccgga cggacccggc cgtcgctgcc caccctggag cacatcgcac
     1921 ggcgcaccgg caagccggtc gaatttttcc tggccgagcc tgtcggcgcc gcggacgatg
     1981 catcggcaag actcatcgag ctcgaggcga tggtcgcgga cggacggaac caggatgcca
     2041 tcgcgctggg cagctcgttg atcgagcgcg gtacatccgc attccgcctc ggacgcatca
     2101 ggtttttcct ggctcaggcc taccaggctt cgtcgcaggc ggagcgagct tccgccctgc
     2161 tcgcggaagc gcgcgcgcac ttcgaggcgg tcaacgatgg cgtgatgctt gccgaatgca
     2221 tcggtgcgca ggcgtccctc gcgaactccc tgcagtcgaa ggacgcattg gcgctcgcgg
     2281 aacaggcgct gtcggtctgt cgcgcattga aaccggtacc tgcgcccacc gaggcgcggc
     2341 tgcttggcat cctcgcgaac gccctggtcg ccgatcgtga atgggaccgc gccgtggccg
     2401 cctacgagca agcgatcgag gtgggtggtt cggtcttcga cctgcgtcgc gttgcccgca
     2461 tgtacagcgc gcttggcctg gcctatcacc aggccggtga ggttgaaccc gcggccaggt
     2521 actcgatgcg ttccgtcgcg ctgctcgagg tgctgcgcga ccgggtggcg ctggcgaggg
     2581 ccgagaacag tcttgcgatg gtccttatgg cgagggggga tttcgcgggt gcgcgcggcc
     2641 acctcgaccg ctcgctcgag ctctgcgacg aaaccgacct cgagtccggc cgcagccgcg
     2701 tgctgctgag cctgtccgag ctctcgatgc agcaaggcaa cgcggccgac gcccgagacg
     2761 gggcgcagca tgcactcgag ctggcagaac gcttcgagga gggagccgac atcgcggagg
     2821 cgcacgtctt gctggggcgg atcgccgacg taggcggcga ggatgagtcc gccgaccacg
     2881 agtttgagca ggccatccgc gggtttgagg cgctgggcct gcacgagagg ctgctgcagt
     2941 gccacgggat ctatgccgag atcctggagc ggcggggcga gctggccaag gcgtacgtcc
     3001 atatgaagga agccctgcag gccacccggc cgggcctgct gcgccgcgag caagaagagg
     3061 agcgcgtcag ctcggcttga ggcccgcccg aagatcgctc ggcggatctt cggcgcccat
     3121 gcggcccatc ttcgggttca gcttctgcgc tgctcgctca cgcatcaacg gatgcgctcg
     3181 ctgcgctgct cggcgcttca cccgaatctg ggccacctgg atcgccgaat ctctcgccga
     3241 gggtttccgt ccgagcctct atagacttcc gattcgtggc caggctcgtc gggatcagcg
     3301 cgctcgag
//
LOCUS       DASPZD010000102         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_193448, whole genome shotgun sequence.
ACCESSION   DASPZD010000102 DASPZD010000000
VERSION     DASPZD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193448"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..>2103)
                     /locus_tag="VEM33_01875"
     CDS             complement(134..>2103)
                     /locus_tag="VEM33_01875"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HYS50607.1"
                     /translation="ARPQRAAKGRAGSPAREKAVETRLFVRKSQLARARTEAPEVLRL
                     NRESMRWFERYFNSRFPFPKYDLVLVPEFAYGGMEHAGATFLREEAVLFPSTPNETDI
                     LARAQLLFHETSHQWFGDLVTMRWFDDLWLKEGFANFMAAKAAEALLPEHSVWNAFHA
                     LKTAAYRTDVTQGTTPIYRPLSNLSGAKSAYGNIVYGKAPAVLRQAEFYVGEPVFRRA
                     VRQFLKKHAYAAADWNDLVAALERASGRKLKRWAQAWVKRRGMPEVRLAWDTDREGRP
                     KNTVLEQHNVLNEGGTWPMKLRVYALSESGLPRSTDALLRGGSTSVRAIDGMPEIDFA
                     FANFGDYGYGRFLLDPMSREAVLARPEIVQGDLLRALVFGSLWESVRDAELAPLDYLD
                     LVVRIAPVEQDPVTLAGLLQRAQVAFLHYASDPQRDAVAEPFEQLLAEGMLHADTPGR
                     RITFLRTFTASAWSESGRSRLKSLLASKLEIPGVKLSSRDRFRAIARLLALDDPEAKE
                     LLSAQIAADSGDDGRRYAFAAAAAERSAEAKRAYFERFFKEQGVPESWIDAALGPFNA
                     VEHAELTQPYLDLALAALPEFKRTRKIFFVNNWLAAFIGGQVDAAALEQVESFARQPE
                     LEPDLKLQLLEAMDGLARTVKIRARFARFLP"
BASE COUNT          367 a          689 c          700 g          347 t
ORIGIN      
        1 aaccctggac ggtcgagaac gggctgctca cgccgactct gaaagtcaaa cgcgagaagg
       61 tgcagaagaa attcgccccc gagatcgagc gcgcctactc cgccggcgcg ccggattgac
      121 gcccacaatc ccgtcagggc aagaacctcg caaacctagc tcggatcttg acggtgcgcg
      181 cgagtccgtc catcgcttcg aggagctgga gcttcaggtc gggctcgagc tccggctgcc
      241 ttgcgaagct ctccacctgt tcaagggcgg cggcgtccac ctgtcctccg atgaaggcgg
      301 cgagccagtt gttgacgaaa aagatcttgc gcgtgcgctt gaattcgggc aaggcggcga
      361 gcgcgagatc gaggtagggc tgggtcagtt cggcgtgctc gaccgcgttg aacgggccta
      421 gcgccgcatc gatccagctt tcgggcacgc cctgttcttt gaagaagcgc tcgaaatagg
      481 cgcgcttcgc ctcggcgctg cgctcggccg cggcggcggc aaaggcgtag cggcggccgt
      541 cgtctccgga atccgcggcg atctgcgcgg agagcagctc tttcgcctcc gggtcatcga
      601 gcgcgagcag ccgcgcgatt gcgcggaagc ggtcgcgcga gctgagcttc accccgggga
      661 tctccagctt gcttgcaagg agcgatttga gccgggagcg gccgctctcc gaccaggcgc
      721 tcgccgtgaa cgtcctgagg aaggtgatgc ggcgccccgg cgtatccgcg tgcagcattc
      781 cctccgcgag aagctgctcg aaaggctccg ccacggcgtc ccgctgcgga tccgatgcat
      841 agtggagaaa agcgacctgc gcgcgttgga gcaggccggc gagcgtcacg gggtcctgct
      901 cgaccggcgc gatgcgaacg acgaggtcga gatagtccag gggcgcaagc tcggcgtccc
      961 gcaccgattc ccagagcgaa ccgaagacga gcgcgcgcag cagatcgccc tgcacgattt
     1021 ccgggcgagc gagcaccgcc tcgcggctca ttgggtcgag caggaaacgg ccgtagccgt
     1081 agtctccgaa attcgcgaag gcgaaatcga tctccggcat gccgtcgatc gcgcgaacgc
     1141 tcgtactccc gccccgcaac aaggcatcgg tcgagcgggg aagcccgctc tcggacaagg
     1201 cgtacaccct cagcttcatc ggccaggttc cgccctcatt gagcacgttg tgctgctcca
     1261 ggaccgtgtt cttcggccgg ccctcacgat cggtgtccca ggcgaggcgc acctccggca
     1321 tgccgcgccg cttgacccag gcttgcgccc agcgcttcag cttgcggccg gaggcacgct
     1381 cgagcgcggc gacgaggtcg ttccaatcgg cggctgcata ggcgtgtttt ttcaggaatt
     1441 gcctcaccgc ccggcggaag accggctcgc cgacgtagaa ctcggcctgg cgcaacaccg
     1501 ccggcgcctt cccgtagacg atgttgccgt aggcggattt cgctccggaa agattggaga
     1561 gcggacggta gatcggcgtc gtcccttgcg tgacgtcggt cctgtaggcg gcggtcttga
     1621 gcgcgtggaa ggcgttccag accgaatgct ccgggagcag cgcctcggcc gccttggccg
     1681 ccatgaaatt ggcgaaccct tccttcagcc acaagtcgtc gaaccagcgc atggttacga
     1741 gatcgccgaa ccactggtgc gaggtctcgt ggaacaggag ttgcgcgcgc gcgaggatgt
     1801 ccgtttcgtt gggcgtggac ggaaacagca ctgcttcctc gcgcagaaac gtcgccccgg
     1861 catgctccat gcctccgtaa gcgaactccg ggacgagcac caggtcgtac ttgggaaacg
     1921 gaaagcgcga gttgaagtag cgctcgaacc aacgcatcga ctcgcgattg agccgcagca
     1981 cctcgggggc ctccgtacgg gcgcgtgcaa gctgcgactt gcgaacgaac agcctggtct
     2041 cgactgcctt ttccctggcc ggagaacccg ctcgcccttt ggcagcccgc tgcggccgcg
     2101 ccg
//
LOCUS       DASPZD010000103         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_192617, whole genome shotgun sequence.
ACCESSION   DASPZD010000103 DASPZD010000000
VERSION     DASPZD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192617"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VEM33_01880"
     CDS             complement(<1..110)
                     /locus_tag="VEM33_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYS50608.1"
                     /translation="MSRDPQGLGLIPMVIEQSGRGERAYDIYSRLLKERVV"
     gene            complement(107..1411)
                     /gene="tig"
                     /locus_tag="VEM33_01885"
     CDS             complement(107..1411)
                     /gene="tig"
                     /locus_tag="VEM33_01885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYS50609.1"
                     /translation="MQTSIEALGRLERRFNMAVPAEQIDREVEQRLRKLSRTVRMDGF
                     RPGKVPLKIVAQHYGPQVRSEVVGDAVQKAFSEVVQEQKLKVAGYPRIERKEGGDGKQ
                     LTFSATFEIYPEVKLGDLAGATIQRPVHAVDDADVDRTLAILRKQRTSWEPVTRASQT
                     GDRVTVDFIGRIDGNEFPGGKGAGVAVVLGEGRMLPEFESGLAGVTAAEHKSFPVGFP
                     ADYPGKEVAGKTANFEVIVQKVETPRLPEIDSEFAKSLGVADGDIAKMREEIRENVQR
                     EVKKKVESEVKQKVMQALIDSTTLELPKSLVEIEMQRMVQQTRADLEARGIKLERLPV
                     KPEALEGQARRRVALGLILGELVKSHELGAKPEQVRSLVTEQAQTYEQPFEVVRWVYS
                     EPQRLSEFEGLAVESNVVSWVLGRAKVEDKAISFDELMGAAA"
     gene            complement(1431..1515)
                     /locus_tag="VEM33_01890"
     tRNA            complement(1431..1515)
                     /locus_tag="VEM33_01890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1479..1481),aa:Leu,seq:tag)
     gene            1619..2428
                     /gene="hpnC"
                     /locus_tag="VEM33_01895"
     CDS             1619..2428
                     /gene="hpnC"
                     /locus_tag="VEM33_01895"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HYS50610.1"
                     /translation="MSVPHYENFPVASVLLPAALRRPVTLIYRFARTADDFADEGDLA
                     PHERLALLAGYRAELRRLEAGRSPESPFFRELGEAVAAHRLPLAPFYDLLDAFSQDVA
                     KQRYASFAELMDYCRRSADPVGRLMLHLYGESDARNVACSDAICSALQLINFWQDVEI
                     DFRKNRIYLPQDDMTRFGVSEDQIAAGDASGGWWDLMRFQIERARSMLLAGAPLATRL
                     SGRVGLEIRAVVQGGSRILEKLERVRGDVFRHRPVLKAFDWPLMLWRALVM"
     gene            2428..3261
                     /gene="hpnD"
                     /locus_tag="VEM33_01900"
     CDS             2428..3261
                     /gene="hpnD"
                     /locus_tag="VEM33_01900"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HYS50611.1"
                     /translation="MTPDEYCQEKAAHSGSSFYYSFLFLPPERRRAITALYAFCREVD
                     DAVDEPSDPSVARETLTWWRGEVARLFAGDPQHPVTRALAPVIAPFGIVRARLEEIMD
                     GMEMDLNQSRYLNFPALRLYCHRVAGVVGALAAGIFGYRNAKTLEYAEKLGLAFQLTN
                     IIRDVGEDARKSRIYLPMDELKEHGVTAADILNANHSESFVRLMRFQAERARRCYDEA
                     LALLPAEDRHAQRPGLMMAAIYRALLDEIAGDDYRVLDQRTALTPIRKLWIAWRTWVT
                     A"
     gene            3237..>4005
                     /locus_tag="VEM33_01905"
     CDS             3237..>4005
                     /locus_tag="VEM33_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYS50612.1"
                     /translation="MADLGHGLSGAAVVGAGWAGLAAAVTLAEQGARVTVFEASRSLG
                     GRARRVSIDGADLDNGQHVLIGAYRETLRLMRKVGADPDELLLRLPLELRYADGFHVR
                     APRLPYPLNLLAALFGARKLAFSEALAAMRFLISLRLGNFRVLPDCSVSRLLDEHAQA
                     GALRTRVWEPLCVSALNTPVERASAQVFVNVLRDGLTGARAASDLLVARTDLGRLFPE
                     PAADYVKARGGAVELGTPVRRIARVPGGFRLNDAQTFA"
BASE COUNT          597 a         1401 c         1270 g          737 t
ORIGIN      
        1 accacccgtt ccttgagcag gcgcgaatag atgtcgtaag cgcgctcgcc ccgcccgctc
       61 tgctcgatca ccatgggaat caggcccagg ccttgcggat cccggctcat gcggctgctc
      121 ccataagttc gtcgaagctg atcgccttat cctccacctt ggctcgcccg aggacccagc
      181 tgacgacgtt cgactcgact gccagtccct cgaactcgct caagcgctgc ggttccgagt
      241 agacccatct taccacttcg aagggctgtt cataagtctg ggcctgctcg gtcaccaacg
      301 agcgcacctg ctcgggcttc gcgccgagct cgtgcgattt caccagctcg ccgaggatga
      361 gtccgagcgc aacgcgccgc cgcgcctgcc cctcgagcgc ctcgggtttc acgggcagcc
      421 tctccagctt gatgccgcgc gcctcgaggt ccgcgcgcgt ctgctgcacc atgcgctgca
      481 tctcgatctc gacgagcgac ttcggcagct cgagcgtcgt cgaatcgatg agcgcctgca
      541 tcactttctg cttgacttcg ctttcgactt ttttcttgac ttcgcgctgg acgttttccc
      601 tgatctcctc gcgcatcttc gcgatgtcgc cgtccgccac gccgagcgat ttcgcgaact
      661 cggaatcgat ctccgggagc ctgggcgtct cgactttctg cacgatcacc tcgaagtttg
      721 cggtcttccc cgcgacttcc ttgcccgggt aatcggcggg gaaacccaca ggaaagctct
      781 tgtgctccgc ggcggtgacg cccgcgagac cggactcgaa ctcgggcagc atgcggccct
      841 cgccgagcac gacggccacg cccgcgccct tgcctccggg aaattcgttc ccgtctatgc
      901 ggccgatgaa atccacggtg acgcggtcgc ccgtctgcga cgcgcgcgtg acgggctccc
      961 agctcgtgcg ctgcttgcgc aggatcgcaa gggtgcggtc gacgtccgca tcgtccaccg
     1021 cgtggacggg ccgctggatc gtcgcgcccg ccaggtcgcc gagcttgacc tccgggtaga
     1081 tctcgaacgt ggcgctgaag gtgagttgtt tcccgtcgcc tccctccttg cgctcgatgc
     1141 gcggataacc ggcgaccttc aatttctgct cctggacgac ttccgaaaac gctttttgca
     1201 cggcgtctcc gaccacttcc gagcgcacct gcggcccgta gtgctgcgcg acgatcttga
     1261 gcgggacctt gcccgggcga aaaccgtcca tgcgcacggt gcgcgagagc ttgcgcagcc
     1321 gctgctcgac ttcgcgatcg atctgctcgg ccggaaccgc catgttgaac ctccgttcca
     1381 gcctgcctaa cgcttcgatg ctcgtttgca ttcccgaaaa ccctgctccc tggtgcgaag
     1441 gaagggactc gaacccccaa gccttgcggc gctggaacct aaatccagtg cgtctaccaa
     1501 ttccgccacc ttcgcgggct gacgacgaaa cggccccgca acgcgcgtgt cattctacag
     1561 caaacccccc tatactgtca gcccgagaag gctgaaaccc tttgaaaaat agggcaatat
     1621 gtcggtccct cattacgaaa acttcccggt cgcctcggtg ctgcttccgg cggcgctgcg
     1681 ccgccccgtg accctcattt accggttcgc acgcacggcg gacgatttcg ccgatgaagg
     1741 cgatctcgcg ccgcacgagc gtctcgcgct ccttgcgggc taccgcgcgg agctgcgccg
     1801 gctcgaagcc ggccgttccc cggagtcgcc ttttttccgg gagctgggcg aagcggtcgc
     1861 ggctcaccgc cttccgcttg cgccgtttta cgacctgctc gatgcgttct cgcaggacgt
     1921 cgccaagcag cgctacgcga gcttcgccga gctgatggat tattgccgcc gttcggccga
     1981 cccggtgggg cgcctgatgc tgcacctgta cggcgaaagc gacgcccgca acgtcgcctg
     2041 ctcggacgcg atctgctccg cgcttcagct catcaacttc tggcaggatg tcgaaatcga
     2101 tttccgcaag aaccgcattt accttccgca ggacgacatg acgaggttcg gcgtcagcga
     2161 agaccagatc gccgcgggcg acgcctcggg cggctggtgg gacctgatgc gcttccagat
     2221 cgagcgcgcg agatcgatgc tgctcgccgg cgcgccgctc gcgacgcgct tgagcggccg
     2281 cgtggggctg gaaatccgcg ccgtggtcca gggagggtcg agaatcctgg agaagctcga
     2341 gcgggtgcgg ggggacgtat tccggcatcg cccggtgttg aaagcgttcg actggccgct
     2401 gatgctctgg cgcgctctgg tgatgtgatg actcccgacg agtattgcca ggaaaaagcc
     2461 gcgcacagcg gctcgagctt ctattactcg ttcctgtttc tgccgcccga gcgccggcgc
     2521 gcgatcaccg cgctctacgc tttctgccgg gaggtcgacg acgcggtgga cgaaccgagc
     2581 gatccttcgg tcgcgcgcga gaccctgacc tggtggcgcg gcgaggtcgc tcggctcttt
     2641 gccggcgacc cgcagcatcc ggtgacgcgc gcgctcgccc cggtgatcgc acctttcggg
     2701 atcgtgcggg cgaggctgga agagatcatg gacggcatgg agatggacct gaaccagagc
     2761 cgttacctga attttccggc gctgcggctc tactgccacc gcgtggcggg cgtggtcggg
     2821 gcgctggcgg cgggcatttt cggctaccgc aacgcgaaga cgctggagta cgccgagaag
     2881 ctcgggctcg cgttccagct caccaacatc atccgcgacg tgggggagga cgcgcgcaag
     2941 agccgcatct atcttcccat ggacgagctg aaggagcacg gcgtgactgc ggcggacatt
     3001 ctcaacgcga atcacagcga gagcttcgtg cgcctgatgc gcttccaggc cgagcgcgcc
     3061 cggcgctgct acgacgaggc gctcgcgctc ctcccggcgg aggaccgcca cgcgcagcgc
     3121 cccgggctga tgatggccgc gatctatcgc gcgctgctcg acgagatcgc cggcgacgat
     3181 taccgggtgc tcgaccagcg cactgcgctc acccccatcc gcaagctctg gatcgcgtgg
     3241 cggacctggg tcacggcttg agcggcgccg cggtcgtcgg cgccggctgg gccggactcg
     3301 ctgctgcggt cacgctcgct gagcaaggcg cccgcgtcac ggtcttcgaa gcttcgcgca
     3361 gcctgggtgg ccgcgctcgc cgcgtgagca tcgacggagc cgacctcgac aacggccagc
     3421 acgtactgat cggcgcgtat cgcgagacgc tgcggttgat gcgcaaggtc ggcgccgacc
     3481 cggacgagct tctcctgcgg ctgccgctcg agctgcgcta cgcggacggc tttcacgtgc
     3541 gcgcgccgcg gctcccctac ccgctcaatc tgctcgccgc gctgttcggc gcgcgaaaac
     3601 tcgccttctc cgaggcgctc gccgcgatgc gcttcctgat ttcgttgcgc cttgggaatt
     3661 tccgcgtgct gcccgactgc agcgtgtcgc gcctgctcga cgagcacgct caagcgggtg
     3721 cgctgcgtac ccgcgtctgg gaacccctgt gcgtctcggc gctcaatacg ccggtcgagc
     3781 gcgcctcggc gcaggtcttc gtgaacgtgc tgcgcgacgg cctcaccgga gcgcgcgcgg
     3841 cgagcgatct gctcgtcgcg cgcaccgacc tcgggcgtct gtttcccgag ccggcggcgg
     3901 actacgtgaa agcgcgcgga ggcgccgtcg agctcggcac gcccgtgcgc cgcatcgcgc
     3961 gcgtccccgg cggattccgc ctcaacgatg cgcagacgtt cgcga
//
LOCUS       DASPZD010000104         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_829076, whole genome shotgun sequence.
ACCESSION   DASPZD010000104 DASPZD010000000
VERSION     DASPZD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829076"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VEM33_01910"
     CDS             complement(<1..864)
                     /locus_tag="VEM33_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HYS50613.1"
                     /translation="MLENLTERLSRIAKTMRGEARLTEANIQGALREVRMALLEADVA
                     LPVVKTFVEHVREAALGTEVVGSLAPGQALVGVVHRELTALMGGEDAALDLAVAPPAV
                     VLLAGLQGSGKTTTAAKLAKMLVTERKKKPLLVSCDVYRPAAIEQLRALAAQVGVDFF
                     PSESGQKPTAIALAALDHARKHYHDVLLVDTAGRLAIDEPMMREIAELHAGLKPVETL
                     FVVDSMQGQDSIKVAKAFGDALPLTGIVLTKLDGDARGGSALSVRQVTGKPIKFAGVS
                     EKPDGLEPFHPE"
     gene            897..1718
                     /gene="ccsA"
                     /locus_tag="VEM33_01915"
     CDS             897..1718
                     /gene="ccsA"
                     /locus_tag="VEM33_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYS50614.1"
                     /translation="MPAILLYAAASLLYAILAFYFWRTHWRGASAPRLARLERVATLA
                     PFALHTWLVYDMLWARPELHFGFGQALSVMLWLAVLIYWIESRFLELEGMQAPILALA
                     AVCAPLPALFPGLAGAPYTHSTEFRLHLVLAMVAYGMFTIAALHALLMTLMERRLHGG
                     ALAGPLAALPPLLTMERLLFQIILVGFVFLTLTLATGIAFSETLFGRAMKFDHKTVFA
                     VASWLIFAALLAGRYLYGWRGRVALRWTLVGFVALLLAYVGSRFVLEVILRRGLV"
     gene            1757..2980
                     /locus_tag="VEM33_01920"
     CDS             1757..2980
                     /locus_tag="VEM33_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HYS50615.1"
                     /translation="MDEIPLAIQFAALVFLLILSGFFAMAETGMMAINRYRLRALERT
                     GHRGARLASELLRRTDRLLSVLLAGNTLVNAAITMIVGVMTIDLFGHDRIVLGIATVV
                     VTLLILVFAEITPKVIGARYPEQTALAASYVLKPLVKASAWSLWIVNLFVRCLLWLLR
                     VPIDPGPQRLSMEELRFLVLESGQFMPKKHQSILVNLFDLEAPTETILAQLTTAYHTR
                     LPAYRGELGNLAGILHLRKVMHLVRAEEFDRKALAALLAEPYFVPAGTPAFAQLQYFQ
                     ENHQRIALVVDEYGELQGLVTLEDIIEEIIGEFTTTAPVKSETYFWDKDGSALVEGSS
                     VLRELNRKLGLALPLDGPKTLNGLILEHLRDIPAAGVAVKIADVPMEIVQTQDRVIKT
                     VRLFRPRVSDLAQAA"
     gene            3189..>4027
                     /locus_tag="VEM33_01925"
     CDS             3189..>4027
                     /locus_tag="VEM33_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYS50616.1"
                     /translation="MATAAAVAKVKEVTYLWEGKDKAGKVIKGQMRAGGQAVVQVTLR
                     RQGIVVTKVHKQRLGRGGRVSEKDVSLFTRQMATMMKAGVPLLQSFDIVGKGHANPAV
                     ARLLMGIKTDVETGSSLASAFRKYPLHFDALYCNLVAAGEQAGILENLLERLATYKEK
                     ILGIKSKIKSALFYPIAIVVVAFIITAVIMIFVIPAFKEVFTNFGADLPAPTLFVMGL
                     SNFFVNYWYVIFPAVIGGVWGFFELWKRSIPVQVYMDRLMLKVPVFGDLIKKSTIARW
                     TRTL"
BASE COUNT          634 a         1350 c         1284 g          759 t
ORIGIN      
        1 ttcggggtga aacggctcca ggccgtccgg cttctcgctc acgcccgcga acttgatcgg
       61 cttccccgtg acctgccgca ccgagagcgc cgagccgccg cgcgcgtcgc catcgagctt
      121 ggtgaggacg atcccggtga gcggcagcgc gtcgccgaaa gccttcgcga ctttgatcga
      181 gtcctggccc tgcatcgaat cgacgacgaa gagggtctcg acgggcttca ggccggcgtg
      241 cagctcggcg atttcccgca tcatcggctc gtcgatcgcc aggcgtcccg ccgtgtcgac
      301 gagcagcacg tcgtggtagt gcttcctcgc atgatcgagc gcggcgagcg cgatggccgt
      361 cggcttctgc ccgctttccg aaggaaagaa atcgacaccg acctgggccg cgagcgccct
      421 cagctgctcg atcgccgcgg gccggtagac gtcgcaggag acgagcagcg gctttttctt
      481 gcgttccgtc acgagcattt tcgcgagctt cgccgcggtg gtcgtcttgc ccgagccctg
      541 caggccggcc aacagcacga ccgcgggcgg cgcgaccgcc agatcgagcg cggcgtcctc
      601 ccctcccatg agcgcggtca gctcgcggtg caccacgccg accagcgcct ggccgggggc
      661 gaggctgccg acgacttccg ttcccagcgc cgcctcgcgc acgtgctcga cgaaggtttt
      721 caccacggga agcgccacgt ccgcctcgag gagcgccatg cgcacttcgc gcagagcgcc
      781 ctggatgttc gcctcggtga ggcgcgcctc gccgcgcatc gtcttcgcga tccgggacag
      841 gcgctcggtc aggttttcaa gcacgggcgg gcggggcgtc ggtgtaaact gcggggatgc
      901 cggcgattct actctacgct gccgcgtctc tcctctacgc gatcctcgcc ttttacttct
      961 ggcgcacgca ctggcgtggc gcgagtgcgc cgcggctcgc gcgcctggag cgggtggcga
     1021 ccctcgcgcc gttcgcgctg catacctggc tcgtctacga catgctgtgg gcgcgtcccg
     1081 agctgcactt cggtttcggg caggccctct cggtgatgct gtggctcgcg gtgctgatct
     1141 actggatcga gagccggttc ctcgagctcg agggcatgca ggcgccgatc ctcgcgctcg
     1201 ccgccgtgtg cgcgccgctg ccggcgctct tcccggggct tgcgggtgcg ccatacaccc
     1261 attcgaccga attccggctg cacctcgtgc tcgcgatggt cgcctacggc atgttcacga
     1321 tcgcggcgct gcatgcgcta ctgatgaccc tgatggagcg gcgactgcac ggcggagctc
     1381 tggccgggcc gctcgcggcg ctgccgcccc tgctgacgat ggaaaggttg ctgttccaga
     1441 tcatcctcgt gggattcgtg ttcctcacgc tgacgctcgc aaccggaatc gcgttttcgg
     1501 aaacgctctt cggccgcgcg atgaaattcg accacaagac cgtgttcgcc gtggcttcct
     1561 ggctcatttt cgcggcgctg cttgccgggc gctatctcta cgggtggcgg ggacgggtcg
     1621 cgctgcgctg gacgctcgtc ggctttgtcg cgctgctgct cgcctatgtc gggagccgct
     1681 tcgtgctgga ggtgatcctg cggcgcggtc tcgtctaggc tatactcgaa ttctcttcgt
     1741 catctaggcg ttcgccttgg acgagatccc cttagcaata cagttcgccg cgctcgtctt
     1801 cctgctgatc ctgtccgggt ttttcgccat ggcggaaacg gggatgatgg cgatcaaccg
     1861 ctaccgcctg cgcgcgctcg agcgcaccgg ccatcgtggc gcgcggctcg cctccgagct
     1921 cctgcgccgg accgacaggc ttctgtcggt gttgctcgcc ggcaacacgc tggtgaacgc
     1981 cgcgatcacg atgatcgtcg gcgtgatgac catcgacctc ttcggccacg acaggatcgt
     2041 tctcggaatc gccaccgtcg tggtgactct gctgatcctc gtgttcgccg agatcacgcc
     2101 gaaggtgatc ggcgcgcgct atcccgagca gaccgccctt gcggcgagct acgtgctcaa
     2161 gccgctcgtc aaagcgtcgg catggtcgtt gtggatcgtc aacctgttcg tccgctgcct
     2221 gctgtggctg ctgcgcgtcc cgatcgaccc agggccgcag cgcctgtcga tggaggagct
     2281 gcgcttcctc gtcctggaat ccgggcagtt catgccgaag aagcaccaga gcattctcgt
     2341 caacctgttc gacctcgagg cgccgaccga gaccatcctc gcgcagctca ccaccgcgta
     2401 ccacacgcgg ctccccgctt accggggcga gctcggcaac ctcgcgggca tcctgcatct
     2461 gcgcaaggtg atgcatctgg tgcgcgcgga ggagttcgac cggaaggcgc tcgccgcact
     2521 gctggcggag ccctatttcg tgccggccgg cacacccgcg ttcgcgcagc tccagtattt
     2581 ccaggagaac caccagcgca tcgcgctcgt cgtggacgag tatggggagc tgcaggggct
     2641 ggtcaccctc gaggacatca tcgaggagat catcggcgaa ttcaccacca ccgcgccggt
     2701 gaaatcggag acctatttct gggacaagga cggcagcgcc ctcgtggagg gctcgagcgt
     2761 gctgcgcgag ctgaaccgca agctcgggct tgcgctgccc ctggacgggc cgaagaccct
     2821 gaacggtctc atcctggaac acctgcggga cattcccgcg gcaggggttg cggtcaagat
     2881 tgccgacgtg ccgatggaga tcgtgcagac ccaggaccgg gtgatcaaga ccgtgcggct
     2941 cttccgcccg cgcgtatccg acctcgcgca ggccgcctga agagctccgc gggtcgcgcg
     3001 gcgcaggcag tatttcacgg tccgcgcacc gcctgtttac gtcttgtctt caatgcattg
     3061 cggagagtac ctgagacttt acaaagagcc cctgcccatg catcatcccg ggaccgtccg
     3121 cgagcgtccc ggcttcgccg gaggaagtcg caggctctgc accggcgaat gaagcaggag
     3181 aaaccgccat ggcaaccgca gcagcagtcg ccaaagtcaa ggaagtcacc tacctttggg
     3241 aaggcaagga caaggccggc aaggtcatca aaggccagat gcgcgcaggc ggccaggcag
     3301 tggtccaagt cacgctgcgc cgccagggca tcgtcgtcac caaggtccac aagcagcgcc
     3361 tcggcagggg cggcagggtc agcgagaagg acgtgagcct ctttacccgc cagatggcga
     3421 cgatgatgaa agccggcgtg ccgctccttc agtctttcga catcgtcggc aagggccacg
     3481 ccaacccggc ggtggcgaga ttgctgatgg ggatcaagac cgacgtggag acgggttcct
     3541 cgctcgcctc ggcgttccgg aagtacccgc ttcacttcga cgccctgtac tgcaacctcg
     3601 ttgcggccgg cgagcaggcc gggattctcg aaaacctcct ggagcggctg gcaacctaca
     3661 aggaaaaaat cctcggcatc aaatcgaaga tcaagtcggc gctgttctac ccgatcgcga
     3721 tcgtcgtcgt tgccttcatc atcaccgcgg tcatcatgat tttcgtgatc ccggcgttca
     3781 aggaagtctt cacgaacttc ggcgccgatc tcccggcccc gaccttgttc gtcatggggc
     3841 tctcgaactt cttcgtcaat tactggtacg tcatcttccc cgccgtcatc ggaggcgtat
     3901 gggggttctt tgagctgtgg aagcgctcga tacccgtcca ggtatacatg gaccggctga
     3961 tgctcaaggt gccggtcttc ggcgacctca tcaagaaatc caccattgcg cgctggaccc
     4021 ggacgct
//
LOCUS       DASPZD010000105         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_795716, whole genome shotgun sequence.
ACCESSION   DASPZD010000105 DASPZD010000000
VERSION     DASPZD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795716"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VEM33_01930"
     CDS             <1..204
                     /locus_tag="VEM33_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50617.1"
                     /translation="DAGGENAPIPRLSIQDLQSQVGGGIFETYFAQQHHITAPISGQT
                     PGHSIETFSVCGIRTSRTTPTPS"
     gene            complement(217..1272)
                     /locus_tag="VEM33_01935"
     CDS             complement(217..1272)
                     /locus_tag="VEM33_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYS50618.1"
                     /translation="MTYLAKPKLHHPTLPKNKVGYTRRDYEGKVSTLCAGCGHDSISA
                     ALIQAFFELDIQPHKVAKLSGIGCSSKTPDYFLGNSHGFNTVHGRMPSVLTGANLANR
                     ELIYLGVSGDGDSASIGLGQFAHVMRRGVNMTYILENNGVYGLTKGQFSATADKGSKA
                     KKGATNKDAAVDTVSLALLMGATFVARSFSGDKAQLIPLVKAAIAHEGAAFIDVISPC
                     VAFNNHAGSTKSYDYVRDHNEAVNRLDFWPSRESINVEYNEGDAIEVPQHDGSIIRLR
                     KTHPDYDPTDRVQALEYVAHHAAKGEVVTGLLYLDTNSEDLHAHLNTVETPFNKLGEK
                     ELCPGAGMLEKINAALR"
     gene            complement(1269..1454)
                     /locus_tag="VEM33_01940"
     CDS             complement(1269..1454)
                     /locus_tag="VEM33_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50619.1"
                     /translation="MEIQGGDDDEDDEDDDEKKKKDKDEDDEDDEDEDEDEDDDDEDT
                     LQVRPAVILSAAKDLLS"
     gene            complement(1457..>2741)
                     /locus_tag="VEM33_01945"
     CDS             complement(1457..>2741)
                     /locus_tag="VEM33_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYS50620.1"
                     /translation="DCPLGIRVRPLKGEANAVGEKIFIEGNAAAALGCVYGGATVCAW
                     YPITPSSSLAEAFQKYCAKLRVDKETGKNKFAIVQGEDELASVGIAVGAGWNGARAFT
                     ATSGPGISLMQEFIGLAYFAEIPVTIIDVQRGGPSTGMPTRTQQSDILACAYASPGDT
                     KHVLLFPEDPHECFEMAAQALDLAERLQTPVFLMTDLDIGMNQRLCKPLEWDDSRQYD
                     RGKVMTAEMLDAGKEFGRYLDVDGDGVPFRTYPGTHPSKGGYFTRGTSKNAYAIYSEQ
                     GPDYKANMQRLLKKFETAKTLVPKPILNASKEPARFGAIYYGSTGPAMAEALDALSRQ
                     GIYVNALRLRSFPFQDEIFDFVASHSKVFVIEQNRDAQLKTLLVNDAGINPASLISVL
                     HYDGTPITARYITGEIAEIVAALNVRPLVKKDKAA"
BASE COUNT          435 a          834 c          897 g          575 t
ORIGIN      
        1 gatgccggtg gtgaaaacgc cccaataccg cgcttgtcga tccaggactt gcagtcgcag
       61 gtgggggggg gaattttcga aacctatttt gcccagcagc accatatcac cgcgcccata
      121 agcggtcaaa cgcccgggca ttctattgaa accttttccg tatgcgggat tcgaactagt
      181 cgaacaactc ctaccccgtc gtagaacgaa gctgatctac ctaagagccg cgtttatctt
      241 ttccagcatc cccgcccccg gacacagctc cttctcgccg agcttgttga acggggtctc
      301 cacggtattg aggtgggcgt ggaggtcttc ggagttggtg tcgaggtaca gcaggccggt
      361 gacgacttcg cctttcgccg cgtggtgggc gacgtattcg agcgcctgga cgcggtcggt
      421 cgggtcgtag tcggggtggg tcttgcgcag gcggatgatc gagccgtcgt gctgcggcac
      481 ttcgatggcg tcgccttcgt tgtattcgac gttgatcgac tcgcgcgaag gccagaagtc
      541 gaggcggttg accgcttcgt tgtggtcgcg cacgtagtcg tagctcttgg tcgagccggc
      601 gtggttgttg aacgcgacgc aagggctgat gacgtcgatg aatgccgcgc cttcgtgcgc
      661 gattgcggct ttgacgagtg ggataagctg cgccttgtcg cccgagaagc tgcgcgcgac
      721 gaaggtggcg cccatcagga gcgcgagcga gaccgtgtcg accgcggcgt ccttgttggt
      781 ggcgcccttc ttggccttcg agcccttgtc ggcggtggca gagaactggc ccttggtgag
      841 gccgtagacg ccgttgttct cgaggatgta ggtcatgttc accccgcgcc gcatgacgtg
      901 cgcgaactgc cccaggccga tcgaggcgga gtcgccgtcg cccgagacgc cgaggtagat
      961 cagctcgcgg ttggcgaggt tggctcccgt caggacgctg ggcattcttc cgtgcaccgt
     1021 gttgaacccg tgcgagttcc cgaggaaata atccggggtc ttggagctgc agccgatgcc
     1081 cgagagcttg gcgaccttgt gcggctggat gtccagctcg aagaacgcct ggatcagcgc
     1141 agccgagatc gagtcgtggc cgcagccggc gcacagcgtc gagaccttgc cttcgtagtc
     1201 gcggcgcgtg tagccgacct tgttcttcgg cagggtcggg tgatggagct tgggtttggc
     1261 gaggtaggtc atgaaagcag atccttcgct gcgctcagga tgacagcagg gcgtacttgg
     1321 agggtatctt cgtcatcgtc gtcttcgtct tcgtcctcgt cttcatcatc ctcgtcatcc
     1381 tcgtccttgt ctttcttctt tttttcgtcg tcatcctcat cgtcttcgtc gtcgtcgccg
     1441 ccctggatct ccatcctcat gcggccttgt ccttcttaac caagggccgc acgttcagcg
     1501 ccgcgacgat ctcggcgatt tcgccggtga tgtagcgcgc ggtgatcggc gtgccgtcgt
     1561 agtgcaggac cgaaataagg ctcgccgggt tgatccccgc gtcgttcacg agcagcgtct
     1621 tcagctgcgc gtcgcggttc tgctcgatca cgaaaacttt cgagtgcgag gcgacgaagt
     1681 cgaagatctc gtcctggaac ggaaacgagc gcaggcggag ggcgttcacg tagatcccct
     1741 gccgcgaaag cgcgtcgagc gcctccgcca tcgcggggcc ggtcgagccg tagtagatcg
     1801 cgccgaagcg cgccggctcc ttggaagcgt tcaggatggg cttgggaacg agcgtcttcg
     1861 cggtctcgaa cttcttcagg agccgctgca tgttggcctt gtagtccggg ccctgctccg
     1921 agtagatcgc gtaggcgttc ttcgaggtgc cgcgcgtgaa gtaaccgcct ttcgaagggt
     1981 gcgtgccggg ataggtgcgg aacggaaccc cgtcgccgtc cacgtcgaga taccttccga
     2041 attccttccc ggcgtccagc atctcggccg tcatcacctt gccgcggtcg tactggcggc
     2101 tgtcgtccca ctcgagcggc ttgcacaggc gctggttcat gccgatgtcg agatcggtca
     2161 tcaggaacac cggcgtctgc agccgctcgg cgaggtcgag cgcctgagcg gccatctcga
     2221 agcattcgtg cgggtcctcc gggaaaagca gcacgtgctt ggtgtcgccg gggctcgcgt
     2281 aagcacaggc gaggatatcg gactgctggg tgcgcgtcgg catgccggtc gaaggcccgc
     2341 cgcgctgcac gtcgatgatc gtcaccggga tctcggcgaa atacgcgagg ccgataaact
     2401 cctgcatcag cgaaatgccc ggacccgaag tcgcggtgaa ggcgcgtgcg ccgttccacc
     2461 ccgccccgac ggcgatgccg accgaggcga gctcgtcctc gccctgcacg atggcgaact
     2521 tgttcttgcc ggtttccttg tccactctca gcttcgcgca atacttctga aaggcctcgg
     2581 cgagcgacga ggacggcgtg atcggatacc acgcgcacac ggtcgccccg ccgtagacgc
     2641 agccgagcgc ggcggcggca ttgccttcga tgaagatctt ttcgccgacg gcgttggcct
     2701 cgccttttaa gggccgcacg cggatcccca gcgggcaatc c
//
LOCUS       DASPZD010000106         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_311994, whole genome shotgun sequence.
ACCESSION   DASPZD010000106 DASPZD010000000
VERSION     DASPZD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311994"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VEM33_01950"
     CDS             complement(<1..832)
                     /locus_tag="VEM33_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate carboxypeptidase"
                     /protein_id="HYS50621.1"
                     /translation="MIGTQAAAQPVEPVLSLAKKEKPALLDTLKELVSIETGSRDLEG
                     LDRTAKLIAARLSALGGKVELVDVSEGAYRMEDTPAKIGKVVHARFEGKGTKKILLLA
                     HMDTVYLRGTLAEQPFRVEGNRAYGLGIADDKNGVALILHVLAMLKAMDFRGYGLVTV
                     LVNADEEMSSPGSRHLLTRLGAEHDAVFSCEASRTDSDNLALATAGIASVILKVKGRA
                     SHAGQAPEQGRNALYELAHQMLQTRDLSNPATGLKMNWTLASGGSVRNAIPAEATGTA
                     D"
     gene            complement(939..1247)
                     /locus_tag="VEM33_01955"
     CDS             complement(939..1247)
                     /locus_tag="VEM33_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50622.1"
                     /translation="MKKNAWYRSNKIAIVGKGARLGLPCDDIEARARRQGLNLSLFPD
                     EAAALRWFHDRRQGSRRAGDGNHVHDEVLRPAIEEQRRREDRRQAPVHIVRGETQVGA
                     "
     gene            1663..>2219
                     /locus_tag="VEM33_01960"
     CDS             1663..>2219
                     /locus_tag="VEM33_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50623.1"
                     /translation="MILLAVSVFAGAGTIHYQTPMLAAFAAEFDTDAAAAGWVATLTF
                     GGFLAGTILIVPLGDRFDKRRLILAELAVLIVALVAMAAAPSLPALALASLVVGITSG
                     FAQLVIPLTAELAPPATRGRVMGALLACLFLGILFGRIVGGLVASYLGWRWTYVIAAT
                     ILLALAPALVLWLPSMPAKTRLGYAR"
BASE COUNT          322 a          765 c          697 g          435 t
ORIGIN      
        1 cgtcagcggt cccggtggcc tctgcgggga tcgcgttgcg gacgctgccg ccgctcgcca
       61 gggtccagtt catcttgagg ccggtcgccg gattcgaaag gtcgcgtgtc tggagcatct
      121 ggtgcgcgag ctcgtagagc gcgttgcgcc cctgctcggg cgcctggccc gcgtgcgagg
      181 cgcgcccttt caccttgagg attaccgacg cgatgcccgc cgtcgcgagc gcgaggttgt
      241 cgctgtcggt acgcgaggcc tcgcaggaga acaccgcgtc gtgctccgcc ccgaggcgcg
      301 tcaggaggtg gcgcgagccg ggcgagctca tttcctcgtc ggcgttgacg agcaccgtga
      361 ccaggccgta gccgcggaag tccatcgcct tcagcatcgc gagcacgtgc aggatcagcg
      421 cgaccccgtt cttgtcgtca gcgatgccca ggccgtaggc gcggttgccc tcgacgcgga
      481 aaggctgctc cgcgagcgtt ccgcgcaggt acaccgtgtc catgtgcgcc agcagcagga
      541 tcttcttcgt tcccttgcct tcgaagcgcg cgtgcacgac cttcccgatc ttcgccggcg
      601 tgtcctccat gcggtacgcc ccctcgctga catccacgag ctcgaccttg ccgcccagtg
      661 cggagaggcg cgcggcgatc agcttcgcgg tgcgatccag cccttcgagg tcgcggctgc
      721 cggtctcgat cgacaccaac tctttcaggg tgtcgaggag cgcgggcttc tccttttttg
      781 cgagcgagag aaccggctcg accggctgcg ctgcagcttg agttccgatc aacgcggttg
      841 cgagaaccgc ggcaatgcaa catcctggat ggcaaatcgg gtggctcacg gctcttcctc
      901 ttcctttcca gcgctcttgc acgccgggca gtgtaccgct atgcgcccac ctgagtctct
      961 ccccgaacga tgtgtaccgg cgcctggcgc cggtcttctc gccggcgctg ctcttcgatc
     1021 gccggccgca gcacctcgtc gtggacgtga tttccatcac cggcgcgtcg cgacccctga
     1081 cgccggtcgt ggaaccagcg cagagctgcc gcctcgtccg gaaagagcga aagattgagt
     1141 ccctgccgtc tcgcgcgcgc ctcgatgtcg tcgcagggca agccgagcct tgcgcccttg
     1201 cccacgatgg ctatcttgtt cgaccggtac caggcatttt tcttcacgtc aggcaaggcg
     1261 ccgatacatc ggtccaaatg gaaaggctta ggcgagcgag catatatcag gacacgggag
     1321 cgcacatttt ccaggcttgc ccgaaaaacc gcgcggagga aggacttcgt ctgcccgcgc
     1381 gattgctggt cgatcagctc cgctctcagg tatccctctt ccaagcttat caggtacctt
     1441 agggcacgcc gacgcgcgga ccagcgtgcc acaatgctct tccacaaagt cgtccgcatg
     1501 ggtcctaacc acggcgtgcc gatcgaagtg gtcacgctgg agcggaaaaa tgaaatacag
     1561 ttcggtgcga agatgaacca ttcggcttag tctcttggcg gcgcagacct tcatttccaa
     1621 aagtccatga cttccccgcc ccgaccactc ggccgaggcc tgatgatcct gctcgcggtc
     1681 tcggtgttcg cgggcgccgg aaccatccac taccagacgc cgatgctcgc ggccttcgcc
     1741 gccgagttcg acaccgacgc ggcggcggcg ggctgggttg cgacgctgac tttcggcggt
     1801 ttcctcgccg gaaccatact gatcgttccg ctcggcgacc gtttcgacaa gcggcggctg
     1861 atcctcgctg aactagcagt gctgatcgtc gccctggtcg cgatggcggc cgcgccttct
     1921 cttcccgcgc tcgccctggc gagcctcgtc gtcgggatca cctccggatt cgcccagctg
     1981 gtcatcccgc tcacggcgga gctcgcgccg ccggcgacgc gcggacgcgt gatgggcgcg
     2041 ctcctcgcgt gcctgtttct cggcattctt ttcgggcgca tcgtaggcgg cctcgtcgcc
     2101 tcctatctcg gttggcggtg gacttacgtg atcgcggcga cgatcctgct cgcgcttgcg
     2161 ccggcgctcg tgctgtggct tccctcgatg cccgcgaaga cgcggctcgg ctacgcccg
//
LOCUS       DASPZD010000107         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1757533, whole genome shotgun sequence.
ACCESSION   DASPZD010000107 DASPZD010000000
VERSION     DASPZD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757533"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VEM33_01965"
     CDS             complement(<1..240)
                     /locus_tag="VEM33_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS50624.1"
                     /translation="MDPGTLLAIDNLRTYFYSRAKRAFIRSVDGVSLGVARGETLGIV
                     GESGSGKSVTALSAAGLVTAGPGVISGRIDLHSNGT"
     gene            complement(246..1139)
                     /locus_tag="VEM33_01970"
     CDS             complement(246..1139)
                     /locus_tag="VEM33_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS50625.1"
                     /translation="MRRYVQTPGPIGRFSELSLAQRAGPLDLLYTGYGLLVVAVVVAS
                     YVLGAFGWLPDDPQAINVGLINAPPFTSGHLLGTDFLGRDLQARLILGIQAYFLPGLL
                     AITISLAGGSILGVLAGYKGGWFDTIITYFDNLLDSFPRLVLILLVIAAFKPDIYYVM
                     VVVGITGMPVIANLIAGKIAFLRQMSFIEAAHALGLPAHTVVLKHILWLNCRALLVIQ
                     ATLGMAEAILVETSLSYLGFGVQEPTPSWGNMVQAGANYFLQGNFWPSTAPALAILAT
                     ILGLQLLGDGLNNLLEGKRRK"
     gene            complement(1148..2110)
                     /locus_tag="VEM33_01975"
     CDS             complement(1148..2110)
                     /locus_tag="VEM33_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS50626.1"
                     /translation="MKLAAIKPISRVLARELAVTALLLLGVSVVVFAILYLAPGDPLN
                     VLLEGQSPRSELALGMREALGVPRTWYGQYAAWFGKMLQGNFGNSIRTGLPVLPELAR
                     VGVYTFTLTLGSLALTLLIAVPIAVHAAAHGGTAGNTAATLAAYVLSAVPVFWFGYIV
                     VYVFIHTFGMFPLISGTAARQEHAWLYFLVPIFVLGVASGTLSEMIRHLREELSRVLA
                     EDYIRTARAKGAPVWRHAFKEGFLIPVTEIMSAKIPFLLGGAVIVEQVFNWPGMGRMA
                     WQAAQDRDFPVIMGIALVAAAFVRAGNLLQRVIFTAVNPRASRE"
     gene            complement(2145..3758)
                     /locus_tag="VEM33_01980"
     CDS             complement(2145..3758)
                     /locus_tag="VEM33_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50627.1"
                     /translation="MKQIFIATVLGLGAMALCIPARAAPKADGRPDGGVLNYSEYGRP
                     ATLDPITSNETISLRITELVFNGLVGIDEKQEIVPELAERWEASKDGRVFTFFLRKDV
                     KWHPREGEPPMPFTADDVVFTYNIMMHPKTITPLKVRYEFIEKVEKTGDYTVVCTLKR
                     PILNALAKFSFKIIPKHALSNPQYLTREDRFAHSPIGTGPYMLQTVTGDGEVVLVSNP
                     EYFKGRAHIDKFIARPFADQNIMAQALMFNAIDMIVLVNPRNLPEVQGDKRFVLQPYN
                     ALSYSFFGYNVRNPLLADRRVRQAITAAVNRQEMLDSFFNGQGTVISGPFAPGSWAYN
                     LDVKPLPFGPERARALLVEAGFRPGADGIMEKDGKKLALSLKVPIEKESEAIKRVVLA
                     FQNYLKNAGIAITVEFKEWQAWKEDVFLEHNFDIMFASWVFDDSADISSLFHSAEIGD
                     WKNNFGGYSNPEVDGLIVESKLTLDHEKRRTINRKLHAILAEEQPYTFLWTLTNYSAY
                     HRKVRRVQIHPYKFFSFADEWFIPASEQK"
BASE COUNT          696 a         1205 c         1256 g          640 t
ORIGIN      
        1 cgtaccgttc gagtgcaggt cgatgcggcc gctgatcacg cccggcccgg cggtgacgag
       61 gcctgcggcc gagagcgcgg tgacgctctt gccgctaccg ctctcgccga caatgccgag
      121 ggtttcgccg cgcgcgacgc ccaggctcac gccgtccacc gagcggatga aggcgcgctt
      181 ggcgcgcgag tagaaatagg tccgcagatt gtcgatggcg agcagcgtcc cggggtccat
      241 tgcgatcact tcctgcgctt gccttcgagc aggttgttga gcccgtcgcc gagcaactgc
      301 agcccgagaa tggtcgcgag gatcgcgagg gccggcgcgg tggagggcca gaagttgccc
      361 tgcaggaaat aattcgcgcc ggcctgaacc atgtttcccc aggaaggcgt gggctcctgc
      421 acgccgaagc cgaggtagga caggctcgtc tcgaccagga tcgcctcggc catgcccagg
      481 gttgcctgga tcaccagcag cgcgcgacaa ttcaaccaca ggatatgctt gagaacgacc
      541 gtatgcgccg gcaagccgag cgcatgcgcg gcctcgataa agctcatctg ccggagaaat
      601 gcgatcttgc ccgcgatcag gttggcgatc accggcatgc cggtgatgcc gaccacgacc
      661 atgacgtaat agatgtcggg cttgaacgcg gcgatcacga gcaggatcag caccaggcgc
      721 gggaacgagt cgaggaggtt gtcgaagtac gtgatgatcg tatcgaacca gccgcccttg
      781 taccccgcca ggacaccgag aatgcttccg ccggcgaggg aaatcgtgat ggcaagcagt
      841 cccggcagaa agtaggcctg gatgccgaga atcagccgcg cctgcagatc gcgtccgagg
      901 aagtcggtgc cgagcaggtg cccggaggtg aatggcggcg cgttgatgag cccgacgttg
      961 atcgcttgag ggtcatcggg cagccagccg aacgccccga gcacgtacga agccaccacc
     1021 accgccacca ccagcaggcc atagcccgtg tagaggagat cgagcggccc cgcgcgctgc
     1081 gcgagcgaca gctccgagaa ccgaccgatg gggccgggcg tctggacata gcgacgcatg
     1141 cttggcccta ttccctcgaa gcgcgcggat tgaccgcggt gaagatgacc cgctgcagca
     1201 ggttgcccgc gcgcacgaac gccgcggcaa cgagggcaat ccccataatg accgggaaat
     1261 cgcgatcctg cgccgcctgc cacgccatgc gccccatgcc gggccagttg aacacctgct
     1321 cgacgatcac cgcaccgccg agcaggaacg ggatcttggc gctcatgatc tcggtcaccg
     1381 ggatcaggaa accttccttg aatgcatggc gccacaccgg ggcgcccttg gcgcgcgcgg
     1441 tgcggatgta gtcttcggcc agcacccgcg acagctcttc gcgcaaatgg cggatcattt
     1501 ccgagagcgt gccgctcgcc accccgagca cgaagatcgg cacgaggaaa tacagccaag
     1561 cgtgctcctg cctcgcggcc gtccccgaga tcagcgggaa catgccgaac gtatggatga
     1621 agacgtagac cacgatgtag ccgaaccaga acaccggcac cgccgagagg acgtacgcgg
     1681 ccaaggtggc ggcggtgttg ccggcggtgc cgccgtgcgc ggccgcgtgc accgcgatcg
     1741 gcaccgcgat caagagcgtg agcgcgagcg agccgagggt gagggtgaac gtgtagacgc
     1801 ccacgcgcgc gagctcgggc agcaccggca ggccggtgcg gatcgaattg ccgaagttgc
     1861 cctgcagcat cttgccgaac cacgccgcgt actggccgta ccaggtgcgc ggcacgccga
     1921 gcgcctcgcg catgccgagt gcgagttccg atcggggcga ctggccctcg agcaaaacgt
     1981 tgagaggatc gccgggcgcg agatagagga tggcgaacac caccacgctc acgccgagca
     2041 acaggagcgc ggtcacagcg agctcgcgcg cgagcacgcg gctgatgggc ttgatcgccg
     2101 cgagcttcat ggtgagcgcc ggcgccggcc ctcaccccgt ctgttcactt ctgctcgctg
     2161 gcgggaataa accactcgtc ggcgaacgag aagaacttgt acgggtggat ctgcacgcgc
     2221 cgcaccttcc tgtggtacgc gctgtagttg gtgagcgtcc aaaggaatgt gtagggctgc
     2281 tcctcggcga ggatcgcgtg aagcttgcgg ttgatcgtcc ggcgcttctc gtggtcgagt
     2341 gtgagcttgc tctcgacgat cagcccatcc acttcggggt tggaatagcc gccgaagttg
     2401 ttcttccagt cgccgatctc ggccgaatgg aacagcgacg agatgtccgc cgagtcgtcg
     2461 aacacccagg acgcgaacat gatgtcgaag ttgtgctcga ggaagacatc ctccttccac
     2521 gcctgccact ccttgaactc gacggtgatc gcgatcccgg cgttcttgag atagttctgg
     2581 aacgcgagca cgacgcgctt gatcgcctcg ctctccttct cgatcgggac cttgagcgag
     2641 agggcgagct tcttgccgtc cttctccatg atcccgtcgg cgcccggcct gaagcccgcc
     2701 tcaacgagca gggcgcgcgc ccgctccggg ccgaacggca agggcttgac gtcgaggttg
     2761 tacgcccagc tgccgggcgc gaacgggccg gagatgacgg tgccctggcc gttgaagaac
     2821 gagtcgagca tctcctggcg gttgaccgct gccgtgatcg cctggcgcac gcgccggtcc
     2881 gcgagcagag ggttgcgcac gttgtagccg aaaaacgagt acgacagggc gttgtacggt
     2941 tgcagcacga agcgcttgtc gccctgcacc tcggggaggt tccgtggatt gaccaggacg
     3001 atcatatcga tcgcgttgaa catcagcgcc tgggccatga tgttctggtc ggcgaacggc
     3061 cgggcgatga acttgtcgat gtgcgcgcgc cccttgaagt actccggatt cgagaccagc
     3121 accacctcgc cgtcaccggt tacggtctgg agcatgtacg ggccggtgcc gatcgggctg
     3181 tgggcgaacc ggtcctcgcg cgtcaggtac tgcgggttcg agagggcgtg cttgggaatg
     3241 atcttgaagc tgaacttcgc cagcgcgttc aggatcgggc gcttgagcgt gcacaccacc
     3301 gtgtagtcgc cggtcttctc gactttctcg atgaactcgt agcgcacctt gagcggcgtg
     3361 atggtcttcg gatgcatcat gatgttgtag gtgaacacca cgtcgtccgc ggtaaagggc
     3421 atgggcggct cgccctcgcg cgggtgccac ttcacgtcct tgcgcaggaa gaaggtgaat
     3481 acgcggccgt ccttcgacgc ctcccagcgc tcggccagct ccggcacgat ttcctgtttc
     3541 tcgtcgatcc cgaccaggcc gttgaacacc agctccgtga tgcgcagcga tatcgtctcg
     3601 ttgctcgtga tcgggtccag cgtcgccggc cggccgtact cgctgtagtt gaggacgccg
     3661 ccgtcggggc ggccgtcggc cttcggcgcg gcgcgcgccg gaatgcacaa cgccatcgcg
     3721 cccaagccca gtaccgtagc aataaaaatc tgtttcatac gctcctcgct tgctgactcc
     3781 gacagttagg gttcttc
//
LOCUS       DASPZD010000108         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1292079, whole genome shotgun sequence.
ACCESSION   DASPZD010000108 DASPZD010000000
VERSION     DASPZD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292079"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VEM33_01985"
     CDS             complement(<1..701)
                     /locus_tag="VEM33_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50628.1"
                     /translation="MTSSQFWMIVPGNAILSALILFAIAMPFLYAARRLMHDFFRSVG
                     NAIGGPLRLGARWLFAAAGDMRERNKTVLLQHGREQVGQLIEREFERISALVTRDLEG
                     YPALQRKLLEELTKVEEDYKKCGEVPPPPPSWTEAVTAIVRVKSEGNEMVQKILEEIK
                     RSVQTIHDKAISEYRRAYESRHKILNGFMPFWRSLDKTFGQVDKKLTGLQDTSSKIDA
                     QMEKYEQINKKTDKA"
     gene            complement(921..1940)
                     /locus_tag="VEM33_01990"
     CDS             complement(921..1940)
                     /locus_tag="VEM33_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50629.1"
                     /translation="MIIDCHGHYTTAPKQLEEWRKRQIAELEDSKHVPSKGSLGIGDD
                     EIRASLEGAQLKLQRERGTDITIFSPRAMGMSHHIGDETTSRHWSEHCNELIRRVCTL
                     YPEKFVGVCQLPQSPGVSPKNCIAELERCVKEFGFIGCNLNPDPSGGYWKEPPLTDKW
                     WYPLYEKMVELEVPAMVHVSASCNPNFHGTGAHYINGDTTAFMQFITADLFRDFPTLK
                     FVIPHGGGAVPYHWGRYRGLAQDMKRPPLKELLMNNVFFDTCVYHQPGIDLLLKVIPA
                     DNILFASEMVGAVKGVDPETGFNYDDTKRYVDRIDWVSKADKEKIFHGNVKKVYPRFA
                     RTAKR"
     gene            complement(1958..>2393)
                     /locus_tag="VEM33_01995"
     CDS             complement(1958..>2393)
                     /locus_tag="VEM33_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYS50630.1"
                     /translation="PPSVERAPEGKELTAMLLAGEIDAAILPAVPTDPRLKPLIHNPA
                     AAAEAWHKKYRALQINHMVAVKESLSRSEPEAVREIYRLLAESKKAAKLPKPGEFDTH
                     PFGLEANRRSLEVVIDTVYQQGMIPRRFAVDELFDDVTRKLG"
BASE COUNT          381 a          740 c          761 g          511 t
ORIGIN      
        1 tcggctttgt cggttttctt gttgatctgc tcgtattttt ccatctgggc gtcgatcttc
       61 gacgaagtgt cctgcagtcc cgttagcttc ttgtccacct gcccgaaagt cttgtcgaga
      121 gagcgccaga acggcatgaa accgttcaag atcttgtggc gcgactcgta ggctcggcgg
      181 tattcggata tggctttgtc gtggatcgtc tggacggagc gcttgatctc ctcgaggatc
      241 ttttgcacca tctcgttgcc ttccgacttg accctgacga tcgccgtgac cgcttcggtc
      301 cacgagggcg gcggcggcgg gacttcgccg catttcttgt agtcctcctc gaccttggtg
      361 agctcttcga ggagcttgcg ctgcaaggcg gggtagcctt ccaggtcgcg cgtcaccagg
      421 gcgctgatgc gctcgaactc gcgttcgatc agctggccca cttgctcgcg cccgtgctgg
      481 agcaggactg tcttgttgcg ctcgcgcatg tctcccgcgg ctgcgaacag ccagcgggct
      541 cccagccgca aggggccgcc gatcgcgttg cccaccgagc ggaagaagtc gtgcatcagc
      601 ctgcgcgccg cgtacaggaa cggcatcgcg atcgcgaaca ggatcagcgc ggacaatatc
      661 gcgttgccgg gcactatcat ccagaattgc gagctggtca cgggaccccc cttcccggcg
      721 gttcggccag gttgtctagg acagcggcat aagatagatc aggcgggcac ggcgcgccag
      781 cgtatttgag gcaatatttc tcgcgcgcag gcggaacccc cggaactgtt ttgatccgat
      841 tgaacatttt ttaacgtgca ggatgtcgcc caagggcgac aggcgggtcg gcgcgcgcca
      901 ttcacgcccg cggaccggcc tcagcgcttc gcggtccttg cgaagcgcgg gtacaccttc
      961 ttcacgttgc cgtggaagat cttctccttg tcggccttgc tcacccagtc gatcctgtcg
     1021 acgtagcgct tggtatcgtc gtagttgaag ccggtctccg ggtccacgcc cttcaccgca
     1081 ccgaccatct ccgaggcgaa gaggatgttg tcggccggga tgactttgag gagcagatcg
     1141 atgccgggct ggtgatagac gcaggtgtcg aagaagacgt tgttcatgag cagctccttc
     1201 agcggcgggc gtttcatgtc ctgcgcgagg ccccggtagc ggccccagtg atagggcacc
     1261 gcgccgccgc cgtgcggaat gacgaatttc agcgtcggga aatcgcggaa taggtccgcg
     1321 gtgatgaact gcatgaacgc ggtcgtgtcg ccgttgatgt agtgcgcgcc ggtcccgtgg
     1381 aaattcggat tgcaggaggc gcttacgtgc accatggccg gcacctccag ctcgaccatc
     1441 ttctcgtaca ggggatacca ccatttgtcg gtgagcggcg gctccttcca atagcccccc
     1501 gacgggtccg gattgagatt gcagccgatg aagccgaatt ccttcacgca gcgctccagc
     1561 tccgcaatgc agttcttcgg cgaaacgccg ggactctgcg gaagctggca cacgccgacg
     1621 aatttctccg ggtatagagt gcagacgcgg cggatcaact cgttgcagtg ctctgaccag
     1681 tgccggctcg tggtctcgtc gccgatgtga tggctcatgc ccatcgcgcg cggcgagaag
     1741 atggtgatgt cggtgccgcg ctcgcgctgc agcttcaact gcgcgccttc gaggctcgcg
     1801 cggatctcgt cgtcgccgat tccgagcgaa cccttcgaag ggacgtgctt ggagtcctcc
     1861 agctccgcga tctggcgctt gcgccattcc tcgagctgct tgggagcggt cgtgtagtgg
     1921 ccgtggcagt cgatgatcat cttgttagtc gtctccgtca gccgagtttg cgcgtcacgt
     1981 cgtcgaacag ctcgtccacc gcaaagcgcc gcggaatcat cccctgctgg tagacggtgt
     2041 cgatgaccac ctcgaggctg cgccggttcg cctcgagacc gaacggatgg gtgtcgaatt
     2101 cgccgggctt gggcagcttc gccgccttct tgctctcggc gaggagccgg tagatttcac
     2161 gcaccgcttc cggctcggat ctcgacaagg attccttcac ggccaccatg tgattgattt
     2221 gcagcgcccg gtacttcttg tgccaggctt cggccgccgc cgccggattg tggatgagcg
     2281 gtttcaggcg ggggtcggtc ggcacggcag gcaggatcgc cgcatcgatc tccccggcaa
     2341 ggagcatcgc cgtcagctcc ttgccctcgg gcgcgcgctc cacggacggc gga
//
LOCUS       DASPZD010000109         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1034197, whole genome shotgun sequence.
ACCESSION   DASPZD010000109 DASPZD010000000
VERSION     DASPZD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034197"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VEM33_02000"
     CDS             complement(<1..266)
                     /locus_tag="VEM33_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HYS50631.1"
                     /translation="MLSLHISQRGFTIVEVLISLVVLGVLLALGTPGFVEWLQNQQIR
                     AAAEATLNGLQVARGEAVRRNTPVRFQLVSDLSSSCVLSSDSPTT"
     gene            complement(260..724)
                     /locus_tag="VEM33_02005"
     CDS             complement(260..724)
                     /locus_tag="VEM33_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HYS50632.1"
                     /translation="MQRPTGFTLIELMVTVAIIAVLAAVAIPNYTDYVRRSKLQEATS
                     NLLAMRTKMEQYFQDNRSYTTPGAPVLAPCSAGSSVPIPTVKYFSITCPVLTATQYTI
                     QADGTDPTLVGITFTINEGNVRATTVTPGSPIAQAGYTPNSNCWTIRKGGQC"
     gene            938..1360
                     /locus_tag="VEM33_02010"
     CDS             938..1360
                     /locus_tag="VEM33_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYS50633.1"
                     /translation="MRKAQNRNRENIAQLLRRHGVAPTHQRIEIAHVLFTRREHLSAD
                     QILKWVNSRYAETSKATVYNTLKLFLERKLIRELVVDPAKIVYDPNTEPHHHLYDVVT
                     GRLTDVPADNIRVVGLPALPPGTVADGVDIIIRTRPQS"
     gene            1490..1565
                     /locus_tag="VEM33_02015"
     tRNA            1490..1565
                     /locus_tag="VEM33_02015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1523..1525,aa:Thr,seq:cgt)
     gene            1629..3164
                     /locus_tag="VEM33_02020"
     CDS             1629..3164
                     /locus_tag="VEM33_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50634.1"
                     /translation="MMKLFGVGASDHPMAEVKGARAILDAIPPGDPFKALEDLNHWLE
                     SVRTWEGFTPEHRAQIVKLVDEAAQVHLRKLQREYLSSQRPSKFLENRLWTVIREWHR
                     QSGLAFAICIDAYATGQKGWEDLKQSAPLLTVRALRALAAQMKWQHMRYGPAENSLWE
                     MVAKIYALSENRGYSQSKVIAYAGLPGETSPEQEFLKAVMFAASSPDSLLPVEIELVE
                     RLIAHLAGSFKLTTKLQPDIVYCFDLANHEPPVRAAHLPHRAPTLRFFAAGGAVKEIE
                     KLAQTVKSTGAVPSGIALGSNYEPEVVLEVLDHLALHWAPKPPERRAQRHRVKSRLTV
                     TYGFDGVRAALDPTGEVQFDPSKVESWIVENVSAGGFGASVPQIKGEWLKIGCLLGLQ
                     PEGGSNWVVGVIRRFQRESAQQGTVGIQTLGRAALPVQVKLQSGQMGTSRDSETAILL
                     NPIESAPEAQLLLRSNVLVAGQNLVLERNGKAYLLLPTGDMERGDDYELVRCRQMVRD
                     TGE"
     gene            complement(3169..3840)
                     /locus_tag="VEM33_02025"
     CDS             complement(3169..3840)
                     /locus_tag="VEM33_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50635.1"
                     /translation="MPDLLDAIEIETGKNPTASIIWLHGLGADGNDFAPIVSELRLPG
                     TAIRFVFPHAPVRPVTINGGMRMRAWYDIADGANAREDARGVRASQAFIETLIGREKE
                     RGAKADRLVLAGFSQGGAIALHTGLRHPERIAGIMALSTYVPVEEKLSAEASTANRDA
                     PIFMAHGSYDPIIPLARAEQSRKLLESLGYPVQWREYGMPHSVCPEELADIGAWLGKV
                     LGPRA"
     gene            complement(3844..4440)
                     /gene="mog"
                     /locus_tag="VEM33_02030"
     CDS             complement(3844..4440)
                     /gene="mog"
                     /locus_tag="VEM33_02030"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HYS50636.1"
                     /translation="MTANRELLIGLVSISDRASSGVYKDEGIPALEEWFSRVLASPWR
                     VEKRLIPDERPVIEATLIDLVDRHGCHLVLTTGGTGPAPRDVTPEATLAIADKIMPGF
                     GEQMRRISLEFVPTAILSRQVAVVRKGSLIINLPGQPKSIRETLEGLKNEDGKPVVAG
                     IFAAVPYCIDLIGGPYIETREDVVKAFRPKSAIRPKAS"
     gene            complement(4427..4975)
                     /gene="yjgA"
                     /locus_tag="VEM33_02035"
     CDS             complement(4427..4975)
                     /gene="yjgA"
                     /locus_tag="VEM33_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HYS50637.1"
                     /translation="MISAHPQLPWNVQAEEKPSKTARKRAMHELQSLGERLVGLNSEQ
                     LAAIALPEDLQDAIERARRITKHEARRRQLQYIGRLMRNVDPEPIREKLRAWDGVSTE
                     ETARVHRIERWREKLLEGDGAIGALVHAHPRIDTQRLRALVRNAREERIAGRPPRAYR
                     ELFRALRDIIADSQPVPEDDGE"
BASE COUNT          884 a         1593 c         1658 g          917 t
ORIGIN      
        1 gtagtcggcg agtcgctcga caagacgcag ctcgagctca agtccgagac gagctggaag
       61 cgcaccgggg tgttgcggcg caccgcctcc ccgcgcgcca cctgcaggcc gttgagcgtt
      121 gcctcggcgg cggcgcggat ctgctggttc tgcagccatt cgacgaagcc cggagtgccc
      181 agcgcgagca gcacgccgag gaccaccagc gagatcagga cctcgacgat agtgaagccg
      241 cgctgcgaga tgtgcaggct cagcactggc cgccctttct gatggtccag cagttcgaat
      301 tgggcgtgta tcctgcctgt gcaatcggcg agcccggagt caccgtcgtg gcgcgaacgt
      361 tgccttcgtt gatcgtgaag gtgatgccca ccaacgtggg gtcggtgccg tcggcttgga
      421 tcgtgtactg ggtggccgtg agcacggggc aggtgatgct gaaatatttc acggtcggaa
      481 tgggcacgct ggagccggcg ctgcacggag ccagcacggg agcgccggga gtggtgtacg
      541 agcggttgtc ctggaagtac tgctccatct tcgtcctcat ggcgagcaga ttggaggtcg
      601 cttcctggag cttgctgcgc ctcacgtagt cggtgtagtt ggggatagcg accgccgcga
      661 ggaccgcgat gatcgcgacc gtgaccatca attcaatcag ggtgaaaccg gttgggcgct
      721 gcatgggaac tcccttcttt ttgcggctca atgataggga actcggggag tccgccgcaa
      781 accttcccga cgagtggctg aaatccgcgt acgtacggca ctagcgcgcg aattctagcg
      841 gaattagttc acgtttccgg agcgcggctc gcgggagaac ttcgtcgatt gcagcctgaa
      901 ttagacaagg tccaaatgct cgtctagaat ggttttcgtg agaaaagctc agaaccggaa
      961 tcgcgaaaat atcgcccagc tcctgcgtcg gcatggcgtc gcgccgacgc accagcgcat
     1021 cgagatcgcc cacgtgctgt tcacgcgacg cgagcatctt tctgcggatc agatcctgaa
     1081 gtgggtcaat tcccgctacg ccgaaacatc gaaggcgacc gtctacaaca cgctgaagct
     1141 gttcctggaa aggaagttga tccgggagct ggtcgtggat ccggccaaaa tcgtctacga
     1201 cccgaacacc gaaccgcatc atcacctcta cgatgtcgtg acgggacggc tcaccgacgt
     1261 ccccgccgac aacatccgcg tcgtcggcct gccggctttg ccgccgggca cggtcgccga
     1321 cggcgtggac atcatcatcc gcacgcgccc gcaatcctag taaaaccggg gacagaccac
     1381 tattttcggc gggtggaatc ctgcggcatt gccgcgaagg aaaatggtgg tctgtccccg
     1441 gttttcccgc tgatataatc cgggctcacc cttcgtgcgc gggacacagg ctggcgtagc
     1501 tcagctggta gagcaactga ttcgtaatca gtaggtcggt ggttcgagtc cactcgccag
     1561 caccatcgga gggctacgaa cagggccacg acgattcttc ctccgggagc gcacgagacg
     1621 gagagtgcat gatgaagctg ttcggagtcg gcgcgtccga tcatcccatg gccgaggtca
     1681 agggggcccg ggcgatcctc gacgccattc cccccggcga tcccttcaag gcgctcgaag
     1741 atctgaatca ctggctggaa tcggtgcgca cctgggaggg cttcacgccc gagcaccgtg
     1801 cgcagatcgt gaagttggtc gacgaggccg cacaggttca ccttcgcaaa ctccagcgag
     1861 agtacctctc ctcgcagcgc ccgtccaagt tcctggaaaa ccggctgtgg acggtgatac
     1921 gcgaatggca tcggcaatcg ggcctcgcgt tcgcgatctg catcgacgct tacgcgaccg
     1981 gccagaaggg ctgggaggac ctgaaacaga gcgcgccgct gctcaccgtg cgcgcgctgc
     2041 gtgcgctcgc cgcgcagatg aaatggcagc acatgcgcta cggccctgcg gagaattccc
     2101 tgtgggaaat ggtcgcaaag atctacgcgc tctccgaaaa ccgcgggtac tcgcagtcca
     2161 aggtgatcgc ctacgcaggc ttgcccgggg agacttcgcc cgagcaggaa tttctcaaag
     2221 cggtgatgtt cgcggcatcg tcgcccgaca gcctgctgcc ggtcgagatc gagctggtcg
     2281 agcggctgat cgcgcatctt gccggctcgt tcaagctcac gaccaagctc cagcccgaca
     2341 tcgtctactg cttcgatctg gcaaaccacg agccgccggt gcgtgccgcg catctgccgc
     2401 accgcgcacc gacgctcagg ttcttcgccg caggcggcgc ggtgaaggag atcgagaagc
     2461 tcgcacagac ggtgaaatcc accggtgcgg tgccgtcggg gatcgcgctg ggcagtaact
     2521 acgaacccga ggtcgtacta gaggttctgg atcaccttgc tcttcactgg gcgcccaagc
     2581 cgccggagag gcgagcccaa cggcacaggg tcaagtcgcg gctcaccgtg acctatggct
     2641 tcgacggagt tcgcgccgcg ctcgacccga ccggtgaggt gcagttcgac ccgagcaagg
     2701 tcgagagctg gatcgtcgag aacgtcagtg ccggaggatt cggagcgagc gtgccgcaga
     2761 tcaagggaga gtggctgaaa atcggctgcc tgctcgggct gcagcccgaa ggcggctcga
     2821 actgggtggt cggcgtgatt cgccgtttcc agcgcgaatc cgcgcagcag gggacggtag
     2881 gaatccagac cctggggcgg gcggcgctgc cggtgcaggt gaagttgcag agcgggcaga
     2941 tgggaaccag ccgggactcg gaaacggcga tccttctcaa tccgatcgaa tccgctcccg
     3001 aagcgcagtt gttgctgcgc tccaacgttc tcgttgccgg gcagaatctc gtgctcgagc
     3061 gaaacgggaa ggcctacctg ctgctgccta ccggggacat ggaacgcggc gacgactacg
     3121 agttggtgcg ctgccgccag atggtccgcg ataccggcga gtaggagttc aggccctggg
     3181 gccgagcacc tttcccagcc aggcgccgat atcggcgagc tcctcgggac acaccgagtg
     3241 cggcatgccg tactcgcgcc actggaccgg gtagccgagc gattccagca gctttcgcga
     3301 ttgctccgcc cgcgcgagcg gaatgatcgg gtcgtagctg ccgtgcgcca tgaagatcgg
     3361 ggcgtcgcgg ttcgccgtgc ttgcctcggc ggagagcttt tcctcgaccg gcacgtaagt
     3421 cgacaaggcc atgattccgg cgatgcgctc ggggtggcgc aagccggtgt gcagcgcgat
     3481 cgcgccgccc tgcgagaatc ccgccagcac cagccggtcc gccttcgcgc cgcgctcttt
     3541 ttccctgccg atcagggttt cgatgaatgc ctgcgaggcg cgcacgccgc gcgcgtcttc
     3601 gcgcgcgttc gcgccgtccg cgatgtcgta ccatgcgcgc atgcgcatgc cgccgttgat
     3661 cgtcaccggc cgcaccgggg catggggaaa aacgaagcgg atcgccgtcc cgggcagtcg
     3721 aagctcggac acgatcggcg cgaagtcgtt gccgtccgca cccagcccgt gcagccagat
     3781 gatgctcgcc gtcgggttct tgccagtttc tatttcgatg gcgtcgagca ggtcgggcat
     3841 aagtcagctc gctttcggcc ggatcgccga cttgggccgg aaggccttca ccacgtcttc
     3901 gcgggtctca atgtaagggc cgccgataag gtcgatgcag taaggcaccg cggcgaagat
     3961 gcccgcgacg accggcttgc cgtcctcgtt tttcaggcct tcgagggttt cgcggatcga
     4021 tttgggctgg ccgggcaggt tgatgatgag cgatcccttg cgcacgactg cgacctgccg
     4081 cgagagaatc gcggttggaa cgaactcgag gctgatcctg cgcatctgct cgccgaatcc
     4141 gggcatgatc ttgtcggcta tcgcgagcgt ggcctcgggc gtgacgtccc ggggagcggg
     4201 gccggtgccg ccggtggtca gcaccaggtg gcaaccgtgc cggtcgacga ggtcgatgag
     4261 cgtcgcttcg atcacgggtc gctcgtcggg aataagccgc ttctccacac gccatgggct
     4321 cgccagaaca cgcgagaacc attcctcgag cgccggtatg ccttcatcct tgtagacgcc
     4381 gctcgaggcg cggtcgctga tcgaaacgag tccgatcagc agctctctat tcgccgtcat
     4441 cttccggcac cggttgcgaa tccgcgatga tatcgcgcag cgcgcgaaac agctcgcgat
     4501 aggctcgcgg cggcctgccc gcgatgcgtt cctcgcgcgc gttgcgcacg agggcgcgca
     4561 ggcgctgggt gtcgatgcgg ggatgggcgt ggaccagcgc gccgatcgcg ccgtcgccct
     4621 caagcaattt ctcgcgccaa cgctcgatgc ggtgaactcg agcggtctct tcggtcgaga
     4681 ccccgtccca ggccctcagc ttctcccgga tcggttccgg atccacgttg cgcataagcc
     4741 tgccgatgta ttgcagctgc cggcgccgcg cctcgtgctt cgtgatgcgc cgcgcccgct
     4801 cgatcgcgtc ctgcaagtct tcgggcagcg cgatcgcagc gagctgctcc gagttgagcc
     4861 cgaccaggcg ctcgccaagg ctctgcagtt cgtgcatggc gcgcttgcgc gcggtcttgc
     4921 tgggcttttc ctcggcctgc acgttccagg gaagctgggg atgagctgat atcatagcgt
     4981 ctttcccgct gcgtccccgc atggccccaa gccgcttcgc ccacgatctc gacgcgttga
     5041 agagcatcgc ct
//
LOCUS       DASPZD010000110         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_115694, whole genome shotgun sequence.
ACCESSION   DASPZD010000110 DASPZD010000000
VERSION     DASPZD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115694"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VEM33_02040"
     CDS             <1..446
                     /locus_tag="VEM33_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HYS50638.1"
                     /translation="HANNVFDVIGRFMHMNVDLFSFVSALNAILAQRLVRLVCPHCGE
                     EGRPDAALLKESGLTEAQAASFRFRAGRGCRECRGSGYKGRKAIAELMILNDELRELI
                     TARAPVRQLKEAARTNGTRFLRDAALEAVKNGESTLEEINRVTFI"
     gene            430..1254
                     /locus_tag="VEM33_02045"
     CDS             430..1254
                     /locus_tag="VEM33_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50639.1"
                     /translation="MLRLSSDRILIALAPDSLALLRVSGGRVSEKRTIACDPAAGSQP
                     WQGAVAALEQVAEETRHANAKVTVVLSNHFARFILVPWSEGLKNAEEETAFVRYCFAK
                     VHGERSKEWDLRLSPTPTGSARIASAIDSSLVQAVRAAFPAAARAKLVSVQPYLMSAF
                     NRWRKDIKGERAWFLLVEPQRACVARLEGGRWSAVRNTRGNFDEPGQWAGLLDRERHR
                     VGGDEASEGVYVHAPLNGNALPVEGEEWSFRSLALAPAEGLAPAESQPFAMALCAL"
     gene            1251..1775
                     /locus_tag="VEM33_02050"
     CDS             1251..1775
                     /locus_tag="VEM33_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50640.1"
                     /translation="MSPHRIELDYVATPRRPQWIGVSVLILALAVSSDMVLRYRNALN
                     EIAAVDAAQGLLSANRRPQRAVSKERLDEEAKIIDAAVRQLSIPWAQMIEAVEAASSS
                     EVTVLQLQPETQQRNLRLTAEAKNREAMLRYVRRLGHTRVLSGVHLVNHHVQVEDPSR
                     PIQFGVQASFRNSR"
     gene            1772..2311
                     /locus_tag="VEM33_02055"
     CDS             1772..2311
                     /locus_tag="VEM33_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50641.1"
                     /translation="MKRAQEWLHSLGSPGVLGIGVLIFCVPFYFSAVSPAERELQAQR
                     SVAERLRSRTPFQPVASEGRAEELRRFYGLFPPLEKLPDELERVYALARSAKIELLQG
                     EYRLEKPPFGMAFYRITLPIRGTYPQIRQFVAAMLKSMPIASLDALRFERKKAGDPQL
                     EAQLRLTVYFRPANEGETP"
     gene            2308..>3779
                     /locus_tag="VEM33_02060"
     CDS             2308..>3779
                     /locus_tag="VEM33_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYS50642.1"
                     /translation="MKPSKTLYATLAVALVAACAGDVLIRDTRQLMSEGRTDEALASL
                     EKASREDPQNYAYRSEYFRLRDFAVAQWLVQADALRAAAQFEPAEELYRRVLKYDAAN
                     SRATQGLAQVDLDKRHRGLVAEADKLVKVEKYRDARDLLATVLAENPVQREARQLQRL
                     IEEKTTKPAIALLQLRSSVTKPISLELKDVPLRTVFDVIARASNLNFLFDKDVRADQR
                     TTIVVRDAQVEDVIKLILATNQLEQKVLNETTALIYPNTPQKLREYQDLVVNSFYVAN
                     ADVRQTANLIRTILKTRDIFIDEKLNLLVMKDTPNAIRLAEKLIAAQDIAEPEVMLEV
                     EVLEVSYNKLLSLGIQFPGSAAISLVGGAATAGAAGTPGVVTLPELRSRDENLVRITF
                     PDPLFLLSLRQTDSRTNVLANPRIRVKNKEKARIHIGDRVPVITTTSAVSGGFVSNSV
                     SYLDVGLQLEVEPLIYLEDDVGIKVGLEVSSITNTIPQAGG"
BASE COUNT          706 a         1269 c         1181 g          623 t
ORIGIN      
        1 tccacgcgaa caacgtattc gacgtgatcg gccgcttcat gcacatgaac gtcgatctgt
       61 tcagcttcgt ctccgcgctg aatgcgatcc tggcgcagcg cctggtgcgg ctcgtctgcc
      121 cgcactgcgg cgaggagggc cggcccgacg cggcactcct caaggaatcc ggcctcacgg
      181 aggcgcaggc tgcttcgttc cgttttcgcg ccgggcgcgg atgccgcgag tgccgtggct
      241 ccggctacaa gggacgcaag gcgatcgccg aactcatgat cctcaacgac gagctgcgcg
      301 agctgatcac ggcccgcgcc ccggtgcgcc agttgaagga agccgcacgc acgaacggca
      361 cccgcttcct gcgcgatgcc gcgctcgagg ccgtgaagaa cggtgaatcg acgctggaag
      421 agatcaaccg tgttacgttt atctagcgac cgtatcctga tcgcgctggc accggattcg
      481 ctcgcgctcc tgcgggtttc cggcggccgt gtgagcgaga aacgcaccat cgcctgcgac
      541 ccggcggcag gcagccagcc ctggcagggc gcagtggccg ccctggagca ggtcgcagaa
      601 gaaacgagac acgcgaatgc gaaggtgacc gtggtgctct ccaaccactt cgcgcgcttc
      661 atcctcgttc cctggagcga aggcctcaag aacgcggagg aagagaccgc tttcgtccgg
      721 tactgtttcg ccaaggtaca cggcgagcga agcaaggaat gggacctgcg gctgagcccg
      781 accccgaccg gctcggcgcg aatcgcgagc gcgatcgatt cgtcgctcgt gcaggccgtg
      841 cgcgccgcgt ttcccgcagc cgccagagcg aagctcgtgt cggtccaacc ctatctgatg
      901 tcggcgttca accggtggcg caaggacatc aagggcgagc gcgcgtggtt tctgctcgtc
      961 gagccgcagc gcgcctgtgt cgcccgcctc gaaggcggac ggtggtcggc ggtgcgcaac
     1021 accagaggaa acttcgacga gccgggccaa tgggccgggc tgctcgatcg cgagcggcat
     1081 cgggtcggcg gagacgaggc gtctgaaggc gtctacgtcc acgcacccct gaacgggaat
     1141 gcccttccgg tcgaaggaga ggaatggagc ttcaggagcc ttgcgctcgc ccccgcggag
     1201 ggcttggctc ccgcggagtc acaaccgttt gccatggcgc tctgcgcact atgagcccgc
     1261 atcgaatcga gctcgactac gtcgcgaccc cgcgccgtcc gcagtggata ggcgtgtcgg
     1321 tgctgatcct cgcgctcgcg gtctcgagcg acatggttct gcgctatcgc aacgccctga
     1381 acgagatcgc tgcggtcgat gcggcgcagg gcctgctcag cgccaaccgg cgaccgcagc
     1441 gagcggtatc caaggaacgg ctcgacgagg aagcgaagat catcgacgcc gcggtgcggc
     1501 agctcagcat tccgtgggcg cagatgatcg aggcggtcga ggcggcctcg agcagcgaag
     1561 tgaccgtgct gcagctgcag ccggaaaccc agcagcgcaa cctgcgcctg acggccgagg
     1621 cgaaaaaccg ggaggccatg ctcaggtacg tgcgccggct cggccatacc cgggtgcttt
     1681 ccggagtgca cctggtcaat catcacgtcc aggtcgaaga tccttcgcgt ccgatccagt
     1741 tcggcgtgca ggcgtccttc aggaacagcc ggtgaagcgc gcccaggaat ggctgcactc
     1801 tctggggtca cccggagtgc ttggcatagg ggtcctcatc ttctgcgtcc cgttctattt
     1861 ttcggcggtc agtccggccg agcgcgagct gcaggcacag cgttcggtcg ccgagcggct
     1921 gcgctcgcgc actccttttc agcccgttgc cagcgagggc cgcgcggaag aactgcgccg
     1981 cttctacggc ctgtttcctc cgctggagaa acttccggac gagctcgagc gcgtgtacgc
     2041 tctcgcgcgc tccgccaaaa tcgaattgct gcagggtgaa taccgcctcg agaaacctcc
     2101 tttcggcatg gcgttctacc gcatcaccct gcccatccgc ggcacttatc cccagatcag
     2161 gcaattcgtc gccgccatgc tcaagagcat gccgattgcg tcgctcgatg ccctgcgctt
     2221 cgagcgaaaa aaggccggcg acccgcaact cgaagcgcag ttgcgtttga ccgtctattt
     2281 ccgtcccgcc aatgaaggcg aaaccccatg aaaccctcca agacccttta tgcgaccctt
     2341 gccgtggcgc tcgtcgcagc ctgtgccggc gacgtactca tcagggacac gcgtcagctg
     2401 atgagcgaag gccgcaccga cgaagcgctc gcgtcgctcg aaaaggcatc gcgggaggat
     2461 cctcaaaact acgcctaccg ctcggagtat ttccgcctgc gcgatttcgc cgtcgcgcag
     2521 tggctcgtcc aggcggacgc cctgcgggct gccgcgcagt tcgagccggc cgaagagcta
     2581 taccgccgcg tgctcaagta cgacgcggcc aactcgcggg cgacgcaggg tctcgcgcaa
     2641 gtcgatctcg acaaacgcca tcgggggctt gtcgccgagg cggacaagct cgtcaaagtg
     2701 gaaaaatacc gtgacgcccg ggacttgctc gcgacggtgc tcgctgaaaa cccggttcag
     2761 cgcgaggcgc gccagctgca gcggctgatc gaggagaaga ccaccaagcc ggcgatcgcc
     2821 ctcctccagc tgaggagctc ggtcaccaag cccatctccc ttgagctgaa agacgtgccc
     2881 ctgcgcaccg tattcgacgt gatcgcgcga gcgtcgaacc tcaacttcct gttcgacaag
     2941 gacgtgcgcg ccgatcagag gaccactatc gtcgtgcgcg acgcgcaggt cgaggacgtc
     3001 atcaagctga tcctcgccac caaccaactc gagcagaaag tcctgaacga gaccacggcg
     3061 ctcatctacc cgaacacgcc gcagaagctg cgcgagtacc aggatctcgt cgtcaatagc
     3121 ttttacgtcg ccaacgccga cgtcaggcag acggcgaacc tgatccgcac catcctcaag
     3181 acgcgcgaca tcttcatcga cgaaaagctc aacctcctgg tgatgaagga cacgccgaac
     3241 gccatccgcc tcgcggaaaa gctgatcgcg gcgcaggata tcgccgagcc cgaggtgatg
     3301 ctcgaagtcg aggtgctcga agtctcctac aacaagctcc tgtcgctggg cattcagttt
     3361 ccgggctcgg cggcgatcag tcttgtgggc ggcgccgcaa ctgccggggc cgccggaacg
     3421 cccggcgttg tcaccctgcc cgagctgcgc tcgcgcgacg agaacctcgt gcgcatcacc
     3481 ttccccgatc cgctgtttct cctctcgctc aggcagaccg acagccggac caacgtgctc
     3541 gccaaccccc gcatccgcgt gaagaacaag gaaaaggcga gaatccacat cggcgaccgc
     3601 gtcccggtga tcaccacgac ctccgcggtg tcaggaggct tcgtctccaa ttcggtgagc
     3661 tacctcgacg tgggtctgca gctcgaggtc gagcccctga tctatctcga ggacgatgtc
     3721 ggtatcaagg tcgggctcga ggtcagcagc atcaccaaca ccatcccgca agccggtgg
//
LOCUS       DASPZD010000111         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_115495, whole genome shotgun sequence.
ACCESSION   DASPZD010000111 DASPZD010000000
VERSION     DASPZD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115495"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1137)
                     /locus_tag="VEM33_02065"
     CDS             complement(175..1137)
                     /locus_tag="VEM33_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50643.1"
                     /translation="MIGIARRLVLVLLVSVAGAATALDYPTRAVKWIVPYPPGGSTDV
                     LARMMSQWLSEKMGQSFVIENKAGGGNNIGVEYVVKSPPDGYTMLLVNPANGINTTLY
                     KNLSFDFIRDIAPVAGLVRAPNVMEVTNSFPARNVAEFIAYCKANPGKINMASSGSGT
                     SVHLSGELFKFMTGCDMLHVPFKGAGPALVELMSGQVHVIFDNLPSSAPHLKGGRIRP
                     LAVTSAEREPSLPGVPTVAETVPGYEATAWFGIGMPKGTPAEIIEKVNAETNRALADP
                     KMQARLAQLGGRPIPGTPQDFGKVIAAETAKWAKVVISSGAKVE"
     gene            complement(1218..1916)
                     /locus_tag="VEM33_02070"
     CDS             complement(1218..1916)
                     /locus_tag="VEM33_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS50644.1"
                     /translation="MILEIRDIETYYGLGHILHGLSLAVAEGEVVALLGRNGAGKTTT
                     LRSVTGLTPPRSGEIRYKGRSIAGLAPHRISRMGIALVPETRGIFSYLNARENLLIAE
                     RRGSRWTMDAVLGRFPALRGRLESKGRLLSGGEQEMLAIARALMTGPELLLLDEPSQG
                     LAPLVVNAVMETIRELKNQGVSMLLVEQNVEMALQLAERVYVIDHGTVVFEGAPGVLR
                     ADHKVTATYLGVGG"
     gene            complement(1913..>2269)
                     /locus_tag="VEM33_02075"
     CDS             complement(1913..>2269)
                     /locus_tag="VEM33_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS50645.1"
                     /translation="LADVGLADKAEQPVREISHGEQRQLELAIALAAAPRVLLLDEPA
                     AGLSPEETKRLVALVRALKGRYTIVLIEHKMDIIMDVSDRISVMHFGSLIAEGTPAEI
                     QRNREVRRAYLGGVAA"
BASE COUNT          360 a          738 c          762 g          409 t
ORIGIN      
        1 ggcaagccag ccggagagga tttcgatggt gactccgagc gctttggcca acgcgatcta
       61 gccaccgcac ctttccaagg cgcgcttgag cgtgcgagtt tgaggcgtac tcaccagcaa
      121 cgaccaattt tccgtccggc acctgtggcc tgtctgtgcg gaggcgtacg tgaatcactc
      181 caccttggcg cccgaggaga tcacgacctt cgcccatttc gccgtctcgg cggcgatgac
      241 cttgccgaag tcctgcggcg tgcccgggat cggcctgcct ccgagctggg cgaggcgcgc
      301 ctgcattttg ggatcggcga gggcgcggtt ggtttcggca ttgaccttct cgatgatctc
      361 cgctggcgtg cctttgggca tcccgatgcc gaaccacgcg gtggcctcgt atccgggaac
      421 ggtttcggcg acggtcggca cgcccggcag ggacggctcg cgctcggccg aggtcaccgc
      481 gagcgggcgg atccgtccgc ccttgagatg gggggcggag gacggcaggt tgtcgaagat
      541 cacatggact tggcctgaca tcagctcgac gagcgcgggc ccggcgcctt tgaacggcac
      601 gtggagcatg tcgcagcccg tcatgaactt gaacagctcg cccgataaat gcaccgaggt
      661 cccgcttccc gaggaagcca tgttgatctt cccggggttc gccttgcagt aggcgatgaa
      721 ttcggcgacg ttcctcgccg gaaaggaatt ggtgacctcc atcacgttcg gcgccctcac
      781 cagccccgcg accggcgcga tgtcgcggat gaagtcgaag gacaggttct tgtacagagt
      841 cgtgttgatc ccgttcgcgg gattgacgag cagcatggtg tagccgtcgg gcggcgactt
      901 caccacatat tccacgccga tattgttgcc gccgcccgcc ttgttctcga tgacgaacga
      961 ctgtcccatc ttttccgaga gccattggga catcatgcgc gcgaggacat cggtcgagcc
     1021 gccgggcgga tagggcacga tccatttcac cgcacgcgtc gggtagtcga gcgcggtcgc
     1081 ggcgccggcg acggagacca ggagaacgag cacaagcctg cgggcgatac cgatcacgga
     1141 acctccttgg gatgaacgat gggtcggatc ttcgtatcgt agcgaaatgt cgcgcgggga
     1201 atccactgtt tctggcgcta gcctccgacc cccaggtaag tcgcggtgac cttatggtcc
     1261 gcgcggagta ctcccggcgc gccttcgaac accaccgtgc cgtggtcgat gacgtagacg
     1321 cgctcggcga gctgcagggc catctcgacg ttctgctcga ccagcagcat gctgacgccc
     1381 tggttcttca gctcccgtat ggtctccatc accgcgttca cgaccagcgg ggcgaggccc
     1441 tgcgaaggct cgtcgagcag cagcagttcc gggccggtca tcagggcgcg ggcgatggcg
     1501 agcatttcct gctcgccgcc ggagagcagc cgccccttgc tctccaggcg cccgcgcagg
     1561 gcggggaagc gcccgagcac cgcgtccatg gtccagcgcg agccgcggcg ctcggcgata
     1621 agcagattct cgcgcgcgtt caggtaggag aagataccgc gcgtttccgg gacgagggcg
     1681 atgcccatcc gcgagatcct gtggggggcc agcccggcga tgctgcggcc cttgtagcgg
     1741 atctcgcccg agcgcggcgg ggtcaggccg gtgacgctgc gcagcgtcgt cgtctttcct
     1801 gctccgttcc ttccgagcag cgcgaccact tcgccttcgg cgacggcgag cgacaggccg
     1861 tggaggatat ggccgaggcc gtaataggtc tcgatgtccc tgatctcgag gatcatgccg
     1921 cgacgcctcc cagataggcg cggcgcacct cgcgattgcg ctggatctcg gcgggcgtcc
     1981 cttccgcgat caggctgccg aagtgcatca ccgagatgcg gtcggagacg tccatgatga
     2041 tgtccatctt gtgctctatc agcacgatcg tgtagcgccc tttcagcgcg cgcaccagcg
     2101 caacgagcct cttcgtctcc tcgggcgaga gcccggccgc cggctcgtcg agcagcagaa
     2161 cgcgcggcgc cgcggcgagc gcgatcgcga gctcgagctg gcgctgctcg ccgtgcgaga
     2221 tctcgcgcac gggttgctcg gccttgtccg cgaggccgac gtcggcgag
//
LOCUS       DASPZD010000112         5668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1778818, whole genome shotgun sequence.
ACCESSION   DASPZD010000112 DASPZD010000000
VERSION     DASPZD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778818"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VEM33_02080"
     CDS             <1..313
                     /locus_tag="VEM33_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYS50646.1"
                     /translation="KACVCMDTGLSHLAAALDVPAITLYGATDTGLIGASGESQIHIK
                     SDRFCSPCRSKTCRYSSGDNPCMELITPDRVHAELQRLTGRALRPVPASPVSASARRA
                     S"
     gene            complement(261..1349)
                     /gene="amrS"
                     /locus_tag="VEM33_02085"
     CDS             complement(261..1349)
                     /gene="amrS"
                     /locus_tag="VEM33_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HYS50647.1"
                     /translation="MSAVREGPYIGRWWHMLPDGRLQCDLCPRDCRLHEGQRGACFVR
                     MRDHDQMVLTTYGRSSGFCIDPVEKKPLNHFYPGSSILSFGTAGCNLACKFCQNWDIS
                     KSRDVDTLADAASPEQLARAALDYGCRSVAFTYNDPVIFAEYAMDVADACREVGVKAV
                     AVTAGYMHDEPRREFYAKMDAANVDLKAFTEEFYFRTCSAHLKDVLDTLVYLEHETEV
                     WFEITTLLIPGRNDGDAEIDAECRWIAKELGPDVPLHFTAFHPDYKMTDIPSTPAATL
                     THARKIALEHGLRYVYTGNVHDPEGGSTFCPRCGSTLLVRDWYRILDYRISAEGKCKA
                     CGAEIPGRFEEFRKPFGPRRIPVRLGLA"
     gene            complement(1346..2737)
                     /gene="amrB"
                     /locus_tag="VEM33_02090"
     CDS             complement(1346..2737)
                     /gene="amrB"
                     /locus_tag="VEM33_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HYS50648.1"
                     /translation="MSSFRNAEVRPPAVASLFYPADARALAEEVSSYLDQTEETPLAP
                     GFPKAVIVPHAGFIYSGPVAASAYDLLRPARGIVTRVVILGPCHRVPVHGLALPRAKA
                     FDTPLGRIPLDEEAIASIRGLPQVVVSAATHAEEHALEVQLPFLQRVLGGFSLVPLVV
                     GDAAPEKVADALERIWGGTETLIVISSDLSHYHSYESARRIDGATVRAILGFDAGISH
                     EQACGATPVAGMLIAAKRKGLGAQLLDCRNSGDTAGDKTRVVGYASFALASEGPRYGA
                     EHGRRLLQIARQSISAALGARGEPGAPAAEESWLRESRASFVTLMLGDELRGCVGALE
                     AQRPLAQDVAENARAAAFEDARFKPLTPHEFGRIDIEVSLLSTPKRLAFEHHADLIGR
                     LRPGVDGIILEQDEEGKRATFLPQVWEGLPDPEQFIAQLRHKAGIAQNTDIRRCRVKR
                     YTVLKWREAELPR"
     gene            complement(2790..3662)
                     /locus_tag="VEM33_02095"
     CDS             complement(2790..3662)
                     /locus_tag="VEM33_02095"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYS50649.1"
                     /translation="MSLSFRCVAAALALCVAAPALAQDLDDSKDNVAGPYVPTPWVIV
                     EEMLKLADIRDEDVVYDLGSGDGRLVITAAKRFGARGVGVELQTELVEMARIGAKHEG
                     VADRVKFVQGDLFQADIRSASVVMLYLLPRFVTRLVPKLRAELRPGTRIVSHDYPLAP
                     WPPDKELSMDVPEKEMISGTSWTRLYYYVVPARVQGAWQLTLPKELAGAPLMLQITQE
                     PHALGGFVRDGGAELTLRDLVVRGGQIRFGLLHKGRLMAFEGTVAGDAMTGEVRARDQ
                     RGPWTARHRAPPQR"
     gene            complement(3659..4156)
                     /locus_tag="VEM33_02100"
     CDS             complement(3659..4156)
                     /locus_tag="VEM33_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HYS50650.1"
                     /translation="MARDTLKLLARRIGARLKKHNLKLATAESCTGGWIAQAVTSVSG
                     SSGWFDRGFVTYSNEAKRELLGVRERTLSRHGAVSRETAEEMAAGALARSRAQVSIAV
                     TGVAGPTGGTKAKPVGTVCFAWSRKRGATGCDTRHFRGGRDRVRRQSVAFALKGLLER
                     LEETG"
     gene            complement(4156..4668)
                     /locus_tag="VEM33_02105"
     CDS             complement(4156..4668)
                     /locus_tag="VEM33_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HYS50651.1"
                     /translation="MILRPTRSFAFAHPAHFIALGFGTGLAPIAPGTVGTLLAFPLFI
                     LLDWLLPPLPLFGAVVPLYFLGVIAVFFVIGVWACARTGRDLGIADHSAMNWDEVVAM
                     LLILVFTPSSWVWWAFAFFAFRFFDVVKPPPIRHIDRAMKGGLGVMFDDIVAAFYTLL
                     LIALVKRILG"
     gene            complement(4665..5624)
                     /gene="thiL"
                     /locus_tag="VEM33_02110"
     CDS             complement(4665..5624)
                     /gene="thiL"
                     /locus_tag="VEM33_02110"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HYS50652.1"
                     /translation="MASEFDIIRKYFTQAAPSAVLGVGDDCALVQCDPGMLLAVSTDM
                     LTAGIHFFPDAEPGKLGHKALAVNLSDLAAMGAGPRWATLALALPEADEKWIAAFAQG
                     FFGLAGRWKTELVGGDTTRGPLAMSVTVIGEVPAGLALRRDAALAGDDIWLSGATGEA
                     ALALAHLRGRVKLEGAGLEACLARLHTPEPRIDLGVRLRGLARSAIDVSDGLLADLGH
                     ILEASRVGAELAWDKLPRAKALADCPDKSLADDCLLAGGDDYELLFTASKLKRVQIET
                     AGRDLKISLARIGAAVQGDPVVRLRDARGKLVSSPRKGFDHFA"
BASE COUNT          967 a         1942 c         1862 g          897 t
ORIGIN      
        1 caaagcctgc gtgtgcatgg acaccggcct ctcccacctc gcggcggcgc tggacgtgcc
       61 cgcgatcacg ctctacgggg cgaccgacac cgggctcatc ggcgcgtcgg gggagtcgca
      121 gattcacatc aagtccgacc gcttctgctc gccctgccgg tcgaagacct gccgctacag
      181 ctcgggggac aacccctgca tggagctgat cacgccggac cgggtccacg ccgaattgca
      241 gcgtctcacc ggccgagcgc tcaggccagt cccagcctca ccggtatccg cctcggcccg
      301 aagggcttcc tgaactcctc gaagcggccc ggaatctccg cgccgcacgc cttgcatttg
      361 ccttcggcgc tgatgcggta gtcgaggatt cggtaccagt cgcgcacgag cagagtgctc
      421 ccgcagcgcg ggcagaatgt gctgccgccc tcggggtcat gcacgttgcc tgtatacacg
      481 tagcgcaggc cgtgctccag cgctatcttg cgcgcgtgcg tcagcgtcgc ggccggcgtg
      541 gaaggaatgt cggtcatctt gtagtcggga tggaaagccg taaagtgcag cggaacgtcg
      601 ggtccgagct ctttggcgat ccagcggcac tcggcgtcga tctccgcgtc gccgtcgttc
      661 ctgcccggaa tgagcagcgt cgtgatctcg aaccagactt cggtttcgtg ctcgaggtag
      721 acgagcgtgt ccagcacgtc cttcaaatgg gcgctgcagg tccgaaagta gaactcctcg
      781 gtgaaagcct tcaagtcgac gttggcggcg tccatcttgg cgtagaactc gcgccgcggc
      841 tcgtcgtgca tgtaaccggc agtcacggcc accgctttga cgccgacctc acggcaggca
      901 tcggcgacgt ccatcgcata ctccgcgaaa atcaccgggt cgttgtaggt aaaggcgacg
      961 ctgcggcacc cgtagtcgag cgctgcgcgc gcgagctgct ccgggcttgc cgcatccgcc
     1021 agcgtgtcga cgtcgcgcga cttggaaatg tcccagttct ggcagaactt gcaggcgaga
     1081 ttgcagcccg cagtgccgaa ggacaggatg ctcgagcccg gatagaaatg attgagcggc
     1141 ttcttttcga ccggatcgat gcagaatccc gaggaacgtc cgtaggtcgt gagcaccatc
     1201 tggtcgtgat cgcgcatgcg gacgaaacac gcgccgcgct ggccctcgtg cagccggcag
     1261 tcgcgcggac acaggtcgca ctgcagcctg ccgtccggga gcatgtgcca ccagcggccg
     1321 atgtacggtc cctctcgcac ggcgctcatc gaggaagctc cgcttcgcgc cacttgagca
     1381 cggtgtaacg cttcacccgg caacgccgga tatcggtgtt ctgcgcaatc ccggctttgt
     1441 gcctgagttg agcgatgaac tgctccggat ccggcaggcc ctcccagacc tgcggcagga
     1501 aggtcgcgcg cttgccttcc tcgtcttgtt cgagaatgat gccgtccacg ccggggcgaa
     1561 gccgcccaat caggtcggcg tggtgctcga acgcgagccg cttgggcgtc gacaagagcg
     1621 atacttcgat gtcgatgcga ccgaactcgt ggggcgtgag cggcttgaag cgcgcgtcct
     1681 cgaacgccgc ggcgcgggcg ttctcggcta cgtcctgagc gagcggccgc tgagcttcga
     1741 gggcgccaac gcagccgcgc agctcatcac ccagcatcag ggtgacgaag cttgcgcgcg
     1801 attcgcgcag ccaggactcc tcggcggccg gtgcgccagg ctccccgcgc gcgcccagcg
     1861 cagcggagat cgattgacgc gcgatttgca gcaaccttct tccgtgctcc gccccgtaac
     1921 gcgggccttc ggatgcgagc gcaaaggagg cgtagccgac gacccgggtc ttgtcgcctg
     1981 cggtgtcgcc cgagttgcgg cagtcgagaa gctgggcccc gagtcccttg cgcttggccg
     2041 cgatcagcat gcccgccacc ggcgtcgccc cgcaggcctg ctcgtgagag attccggcgt
     2101 cgaagccgag aatcgcccga acggtggcgc cgtcgatcct gcgtgcgctc tcgtacgaat
     2161 ggtagtgcga gagatccgag ctgatcacga tcagcgtttc ggttcctccc cagatgcgct
     2221 cgagcgcatc ggctactttc tccggggccg cgtcgcccac caccagcggc acaagggaaa
     2281 atccgcccag gacccgttgc agaaacggca gctgcacttc cagcgcgtgc tcttcggcgt
     2341 gcgtcgccgc cgataccacg acctggggca ggccgcggat cgaggcgatc gcctcctcgt
     2401 ccagcgggat ccggccaagc ggcgtgtcga atgcttttgc gcgcggcagc gcgaggccat
     2461 gcacggggac ccggtggcag ggaccgagga tcacgacgcg cgtgacgata ccccgcgcgg
     2521 gacgtagtag atcataggcg ctcgcagcga ccggcccgga atagatgaaa ccggcgtgag
     2581 ggacgatcac cgccttgggg aatccgggcg cgagaggcgt ctcctccgtc tggtcgagat
     2641 aggaggagac ctcttcggcg agtgcgcgcg cgtccgcggg atagaacaag ctcgcgaccg
     2701 cgggcgggcg cacctcggcg ttacggaagg agctcatgct ctcaatatag agcacgggcc
     2761 tcccggaccc aagccccgcc cgcgcgccgt cagcgttgcg gcggtgcgcg gtgccgggcg
     2821 gtccagggcc cgcgctggtc acgagcccgc acctcgcccg tcatggcgtc cccggccaca
     2881 gtcccctcga acgccatcag ccggcccttg tggagcaacc cgaagcggat ctgcccgccc
     2941 cgcaccacca ggtccctgag ggtgagctcg gcgccgccat cgcgcacgaa tccgccgagg
     3001 gcgtggggtt cctgggtgat ctgcagcatg agcggagcac ccgcgagctc tttgggcaag
     3061 gtgagttgcc acgcgccctg cacgcgcgcc gggacgacgt aatagtagag cctcgtccag
     3121 gacgtgccgc tgatcatctc cttttcgggg acatccatcg aaagctcctt gtcgggcggc
     3181 cagggagcga gcggataatc gtgggacacg atgcgcgttc cgggacgcaa ttccgcgcgc
     3241 agcttcggca ccaggcgcgt gacgaagcgc ggcagcaggt acagcatcac cacgctcgcc
     3301 gaccggatat cggcctggaa caggtcgccc tgaacgaact tgacgcgatc cgcaacgccc
     3361 tcgtgtttcg cgccgattct cgccatttcg acgagctccg tctggagctc gacgccgacc
     3421 ccgcgcgcgc cgaagcgctt ggccgccgtg atcaccagcc tcccgtctcc cgaaccgagg
     3481 tcgtagacca cgtcctcgtc gcggatatcg gcgagtttca gcatctcctc cacgatcacc
     3541 cacggcgtgg gtacgtaggg gccggcgacg ttgtccttcg agtcgtcgag gtcctgcgcg
     3601 agtgcgggcg cggcgacgca aagcgcgagc gcggctgcaa cgcagcggaa agacaaactc
     3661 atcctgtttc ctccagccgt tcgagcaggc ccttcagcgc gaacgccacc gactgccggc
     3721 gcaccctgtc ccggccgccg cggaaatgcc gggtatcgca acccgtcgca ccccgtttcc
     3781 gcgaccaggc gaaacacacc gtgccgacgg gtttcgcctt cgtgccgccg gtcggccccg
     3841 ctacgccggt cacggcgatc gaaacctgcg cgcggctgcg cgcgagcgca cctgcggcca
     3901 tttcttccgc ggtttcccgg ctcaccgcgc cgtgccgcga aagagtccgc tcgcgcacgc
     3961 cgagcagctc cctcttggcc tcgttggagt aagtcacgaa cccgcggtcg aaccacccgg
     4021 agctccccga cacggacgtc accgcctggg caatccaacc gccggtgcaa gactcggccg
     4081 tggcgagctt cagattgtgt ttcttcaggc gcgcgccgat ccgtctcgct agcagcttga
     4141 gcgtatcgcg agccatcagc cgaggattct tttcacgaga gcgataagga gcagggtgta
     4201 gaacgccgcg acgatgtcgt cgaacatcac gcccaggcct cctttcatcg cacggtcgat
     4261 gtggcggatc ggcggcggct tgaccacgtc gaagaagcgg aacgcgaaga acgcaaaggc
     4321 ccaccaaacc cagctcgacg gtgtaaacac caggatcagc agcatcgcga ccacctcgtc
     4381 ccagttcatt gcgctgtgat cggcgatgcc cagatcgcgc ccggttcgag cgcaggccca
     4441 gacgccgatt acgaagaaaa cagcgatgac ccccaaaaaa tacagcggaa cgaccgcccc
     4501 gaagagaggc agcggcggca gcagccagtc gagcaggatg aaaagcggaa acgcgagcag
     4561 cgtgccgaca gtacctgggg cgatcggcgc gagcccggtt ccgaacccga gcgcgatgaa
     4621 gtgggccggg tgagcgaagg cgaagcttcg ggtgggccgc aggatcatgc gaagtgatcg
     4681 aaacccttgc gcggactgga aacgagcttg ccccgggcgt cgcgcagtct caccacggga
     4741 tctccctgta ccgcggcgcc gatacgagcg agcgaaattt tgaggtccct tccggcggtt
     4801 tcgatctgca cgcgcttgag cttcgacgcg gtgaacagca gctcgtaatc gtcgccgccg
     4861 gcgagcaggc aatcgtcggc gagcgatttg tccgggcagt cggcgagcgc cttcgcccgc
     4921 ggcagcttgt cccaggcgag ctcggcgccg acgcgggacg cttccaggat gtgaccgaga
     4981 tcggcgagca ggccgtctga aacgtcgatt gcgctgcgcg caaggccgcg cagccgcacg
     5041 ccgagatcga tgcgcggctc cggcgtgtgc agccgggcga ggcaggcttc gagccccgcg
     5101 ccttccagct tcacgcgccc tctgaggtgg gcgagagcga gcgccgcttc tccggtggcg
     5161 cccgagagcc agatatcgtc cccggcgagc gccgcgtcgc gccgcagcgc aaggcccgcc
     5221 ggcacctcgc ctatcacggt gaccgacatc gcgagcggcc ccctcgtcgt gtccccgccg
     5281 acgagctcgg ttttccagcg gcccgcgaga ccgaaaaacc cctgcgcgaa cgcggcgatc
     5341 cacttctcgt ccgcctcggg cagcgcgagc gcgagcgtgg cccagcgcgg gccggcgccc
     5401 atcgcggcga gatccgagag gttgaccgcc aacgccttgt gcccgagctt cccgggttcg
     5461 gcgtccggga agaaatggat tcccgcggtg agcatgtcgg tcgaaacggc gagcagcatt
     5521 ccggggtcgc attggacgag cgcgcagtcg tcgcccaccc ccagcaccgc gctcggggcc
     5581 gcctgggtaa agtacttgcg gatgatgtcg aactcggaag ccacgtcctc aggctttctt
     5641 ctcctgcggt cgcagctccg gcacgagc
//
LOCUS       DASPZD010000113         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_70096, whole genome shotgun sequence.
ACCESSION   DASPZD010000113 DASPZD010000000
VERSION     DASPZD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70096"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VEM33_02115"
     CDS             complement(<1..464)
                     /locus_tag="VEM33_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYS50653.1"
                     /translation="MTDGYSLREVGKLLGLPRSVVNGLIEAGFVVPARGPRREYRFSF
                     HDLVLLRAAQGLSEAKIPAARILRALRRLRAQLPDKVPLAGLRIGAVGDAVVVSEGEA
                     QWRPDNGQYVFQFQVASPGGRLAFIEPIPPATAPHADWFERGSALEAAEPEKA"
     gene            complement(685..1575)
                     /locus_tag="VEM33_02120"
     CDS             complement(685..1575)
                     /locus_tag="VEM33_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYS50654.1"
                     /translation="MASVTVTFGMVSIPVKLYAATSPQAGISFNLLHGACGSRLKQQY
                     LCAKEGVVVERADMVKGYEFAKDQYVTFTAEELKELEEKGTQMIEISEFVPADSIDPI
                     YYDKAYFLGPDKGGAKPYALLAESMRQSGQTAVGRYAARGKQYIVQLRAVPGGLVMQQ
                     LLYAPEVRTIEDVGIETAPVRDTELALAKQLIAQISSKQFDPSQYEDEVRKRIETAVQ
                     KKVEGEEIAISPSAPEGGAQVIDLMDALRASLEKTSGKRAPARTAAPEGAPAELKTPQ
                     RKPAKKVAAEPEPRRKVGKR"
     gene            1670..>3301
                     /gene="ligD"
                     /locus_tag="VEM33_02125"
     CDS             1670..>3301
                     /gene="ligD"
                     /locus_tag="VEM33_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYS50655.1"
                     /translation="MALERYRQKRDFRVTPEPKGKVGKRKSRALSFVIQKHAATRLHY
                     DFRLELNGVLLSWAVPKGPSLDPNDKRLAMHVEDHPLEYGEFEGVIPPKQYGAGTVML
                     WDRGTWEPKSDPDEGYTKGHLKFELHGEKVKGGWNLVRTRSSKYGDGDKSWLLFKEAD
                     DFARLGPEALIVDERPDSVATGRSLDEIKAAKDRVWHSNQSVAANVKGGAIGKPKTKV
                     SANLTNLKGARERALPAIVEAQLATLVKSPPGGAAWVHEIKYDGYRMLCRIADGEVQM
                     VSRNGKDWTAAFPSIAHVLARLPVETAWVDGEVVVVDAQGRSSFQALQNALSGDNAKA
                     LSFFGFDLLYLNGFDLRGVTLVERKRLLKELLSTAPATIRYSEHFAAPGASFLEKVCD
                     LGLEGMVSKRADLPYQAGRGAAWQKTKCEQRQEMVIGGFTDPEGSREGFGALLLGVYE
                     PDGRLAYSGKVGTGFNSASLATVHRALLPLVQKEPPFHNPPRGAEARRAHWVKPALVA
                     EVSFTEWTDDGTLRHPSFQGLREDKPAKDVVRERVA"
BASE COUNT          608 a         1044 c         1039 g          610 t
ORIGIN      
        1 gccttctcgg gttccgcggc ttcgagcgct gacccgcgtt cgaaccaatc cgcgtgcggc
       61 gccgtggctg ggggtatagg ctcgatgaac gcgagccgtc cacctggcga tgccacttgg
      121 aactggaaca cgtactgccc gttgtcgggc cgccactgcg cctcgccttc gctcacgacg
      181 acggcgtcgc cgaccgcccc gatccgcaat cccgccagcg gcactttgtc tggaagttgg
      241 gcgcgcaacc ggcgcagtgc acgcaggatt cgcgccgctg ggatcttcgc ctcggagagg
      301 ccttgcgcag cgcgcagcag caccagatcg tggaaagaga agcggtattc gcgccgcggc
      361 ccccttgcag ggaccacaaa tccggcttcg atgagcccgt taacgacgga acgggggagt
      421 ccgagcagct taccgacttc gcgcagcgag taaccgtcag tcacaacttg aatggacccc
      481 cacgcttgcg gctatcgccg cgctgccccc tgaggggggc gtaattcgcg ccgcgggata
      541 gtgacgcgag cagccggcga ggggccccgc gacgcgggga tcgacggcca acgcgagtcg
      601 tcacacgagg ccggctcttg acgccgtgac tcgcgtcgcc aatccaatga ggccgaggcc
      661 cggcggcgct catcgatata cgccctaccg cttgccgact ttgcgacgtg gctcgggctc
      721 ggcggcaacc ttcttcgccg gtttgcgctg tggagttttg agctccgcgg gagcgccttc
      781 cggagcggct gtcctggccg gcgcgcgctt gcccgatgtt ttctcgaggc tcgcgcgtaa
      841 tgcgtccatc aaatcgatca cttgggcgcc gccttcgggc gcgctcggcg aaatggcgat
      901 ctcctcgccc tcgactttct tttgcaccgc cgtttcgatc cgcttgcgca cttcgtcttc
      961 gtattggctc gggtcgaatt gcttggacga aatttgcgcg atcagctgtt tcgccagcgc
     1021 gagctcggtg tctcggaccg gcgccgtttc gatcccgacg tcttcaatcg tgcgtacctc
     1081 gggtgcgtaa agaagctgct gcattaccag tccgcccggc accgcgcgca actgaacaat
     1141 gtactgcttg ccgcgtgccg cgtagcgccc caccgccgtc tgcccgctct gccgcatgct
     1201 ttccgccagc agcgcgtagg gtttcgcgcc gcccttgtcc ggaccgagga agtaggcctt
     1261 gtcgtagtag atcgggtcga tactgtcggc aggaacgaac tcgctgatct cgatcatctg
     1321 cgtgcccttt tcctccagct ccttcagctc ctccgcagta aacgtgacat attggtcctt
     1381 ggcgaattca tagcctttga ccatgtcagc ccgctcgacg acgacgcctt ccttggcgca
     1441 caggtactgt tgtttcagcc tcgatccgca ggcgccgtgc agcaggttga acgagatgcc
     1501 cgcctggggc gaagtcgcgg cgtacagctt gaccgggatg gaaaccattc caaaggtgac
     1561 ggtgactgaa gccaatgcac gagccgccat gaggggcctc tcgggtaaat gggacggtca
     1621 tcgtgccaaa atgccacgca atcttcaact gaattgattc gcggacgcca tggcgcttga
     1681 acgttatcgg caaaagcgcg actttcgggt cacgcccgag ccaaaaggca aagtcgggaa
     1741 gcgcaaatcg cgcgctttgt cgtttgtcat ccagaagcac gcggcaaccc ggctccatta
     1801 cgactttcgg ctggagctga acggcgtact gctgagctgg gcagtgccga aggggcccag
     1861 cctcgacccc aacgacaagc gtctcgcgat gcatgtcgaa gatcatccgc tggagtacgg
     1921 tgaattcgag ggtgtgatcc cgcccaagca atacggcgcc gggaccgtga tgctgtggga
     1981 tcggggcaca tgggaaccga agagcgatcc cgacgagggc tatacaaagg gccacctcaa
     2041 gttcgagctg cacggggaaa aggtcaaagg gggttggaat ctcgtgcgca ctcgcagcag
     2101 caaatacggc gacggcgata agagctggct gctgttcaaa gaagccgacg acttcgcacg
     2161 cctggggccc gaggcgctga tcgtcgacga gcggccggac agcgtggcga cggggcggag
     2221 tctcgacgag atcaaggccg cgaaggatcg ggtctggcac tcgaatcagt cggtcgcggc
     2281 aaacgtcaaa ggcggcgcaa tcggcaaacc caagacgaag gtctcggcga acctcacaaa
     2341 tctcaaaggc gctcgcgagc gcgcccttcc cgccatcgtc gaggcgcagc tcgcgacgtt
     2401 agtcaaatcg cctcccggcg gcgccgcgtg ggtgcacgaa atcaaatacg acgggtatcg
     2461 aatgctgtgc cgcatcgcgg acggcgaagt tcagatggtg tcgcgcaatg gcaaggattg
     2521 gaccgccgcg tttccgtcga ttgcccacgt gctcgctcgg cttcccgtgg agaccgcgtg
     2581 ggtcgacggc gaagtggtcg tcgtcgatgc gcagggtcga agcagcttcc aagcgttgca
     2641 gaacgccctc tccggggaca atgccaaagc gctcagtttt ttcggcttcg acctccttta
     2701 cctgaatggc ttcgatctgc gcggcgtcac gctggtcgaa cgaaagcgcc tccttaagga
     2761 attactttca acggcgccgg caaccattcg ttacagcgag catttcgccg cgcctggcgc
     2821 gtcgttcctc gaaaaagtct gcgacttagg gctcgaaggc atggtgtcca agcgggcgga
     2881 cctcccatac caggccggcc gtggcgccgc atggcagaaa accaaatgcg agcaacgcca
     2941 agagatggtg attggcgggt tcaccgaccc cgaaggatcg cgcgaaggct tcggcgcgtt
     3001 gttgctcggc gtctacgaac cggatggccg ccttgcgtat tcaggcaaag tcggaacggg
     3061 tttcaattcg gcgtcgctcg ccaccgtcca tcgcgcgctg cttccgctcg ttcagaagga
     3121 gccgcctttt cataacccgc cgcgtggcgc cgaagcgcgt cgagcacact gggtgaagcc
     3181 ggcgctcgtc gccgaagtgt cgttcaccga atggaccgac gacggaacgt tgcgccaccc
     3241 atctttccag ggtcttcgcg aagacaagcc ggctaaggac gttgtacgcg agcgcgtcgc
     3301 c
//
LOCUS       DASPZD010000114         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1199702, whole genome shotgun sequence.
ACCESSION   DASPZD010000114 DASPZD010000000
VERSION     DASPZD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199702"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VEM33_02130"
     CDS             <1..689
                     /locus_tag="VEM33_02130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HYS50656.1"
                     /translation="VSSSTKGETLLDTVDNLSAMHADMFVVRHSSSGAAHLIASHVAS
                     HVHVINAGDGRHAHPTQGLLDIYTIRHYKKDFTGLSVAIVGDVLHSRVARSLIHALTT
                     LGAPDVRVIGPRTLLPTGVEQLGVSVHHDMCEGLAGCDVVVMLRLQNERMKGPLLPSA
                     QEFFKSYGLTPQKLARAKPDAIVMHPGPMNRGVEIDSSVADGAHSVILPQVTFGIAVR
                     MAVMSILAGN"
     gene            692..997
                     /locus_tag="VEM33_02135"
     CDS             692..997
                     /locus_tag="VEM33_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS50657.1"
                     /translation="MTKSSPPALDSAQVPEQSTTGYPEPYKARVAGRHKRRLGDAAGL
                     KNFGVNLTRLDPGAESSMRHWHSKQDEFIYVLEGELTLVTDAGRQKLKRGMAAGFPA"
     gene            1165..>2137
                     /locus_tag="VEM33_02140"
     CDS             1165..>2137
                     /locus_tag="VEM33_02140"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYS50658.1"
                     /translation="MSKVHIKSGRVIDPKNRVDAKQDVFIADGKIAALGKAPAGFRAE
                     RTIAADALVVCPGLIDLSARLREPGFEYKATLASEMQAAVAGGVTSLACPPDTDPPLD
                     EPGLVEMLKYRAQSLNLAHVYPLGALTWKLGGERLTEMAVLRDAGCVAFSHADTPLPD
                     TQMLWRALQYAATFGIAVWLRPQDGFLARGGVAHDGEVATRLGLQGIPAFAETIALAT
                     ILQLVRATRTRVHLCRLSTAEGVAMLRVAKKEGLPVSCDASAHHAHLSEIDLGYFDSH
                     CNVVPPFRSLRDRDALRAGLADGTIDALCSDHAPVDEDAKQLPFAESE"
BASE COUNT          378 a          738 c          693 g          328 t
ORIGIN      
        1 ccgtgtcgtc ttcgaccaag ggggaaaccc tgctcgacac ggtcgacaac ctttccgcta
       61 tgcacgcgga catgttcgtc gtgcggcatt cctccagcgg cgccgcccac ctgatcgcga
      121 gccacgtcgc ctcgcacgtg cacgtgatca atgcgggcga cggtcgccat gcgcacccga
      181 cgcagggact gctcgacatc tacaccatcc gccactacaa gaaggatttc accgggctct
      241 cggtcgccat cgtcggagac gtgctgcatt cgcgcgtcgc gcgctcgctg atacacgcgc
      301 tcaccactct gggcgcaccc gacgtgcgcg tgatcggccc gaggaccttg ctgcccacgg
      361 gggtggagca gctcggcgtc tccgtgcatc acgacatgtg cgaggggctc gcaggttgcg
      421 acgtcgtggt gatgctgcgc ctgcaaaacg agcgcatgaa agggccgctg ctgccctcgg
      481 cccaggaatt tttcaagtcc tacggcttga cgccgcagaa gctcgcccgc gccaagcccg
      541 acgccatcgt tatgcacccc gggccgatga accggggcgt cgagatcgac tcttcggtcg
      601 ccgacggcgc gcactcggtg atcctgccgc aggtcacatt cggcatcgcc gtgcgcatgg
      661 cggtgatgag catcctcgcc ggaaactgac catgacgaaa tccagcccgc ccgccctcga
      721 ttctgcgcaa gtgcccgagc agtcgaccac cggctatccg gagccgtaca aagcccgagt
      781 cgcgggccgg cacaagcggc gcctcggcga tgcggccggg ctgaagaact tcggcgtcaa
      841 cctcacgcgg cttgacccgg gcgccgagtc ctcgatgcgg cactggcact ccaagcagga
      901 tgagttcatc tacgtgctcg agggcgagct cacgctcgtc accgatgccg ggaggcaaaa
      961 gctcaagcgc ggaatggcgg ccggatttcc agcgtgaagg gccgacggac atcagctcgt
     1021 caacgagaca aagaagccgg cgctctacct ggagatcggc gatcgcaccc ccgggcacgg
     1081 agccgcctac gccgacgtgg acctggcggc gcgcctggta gacgggaaat gggtcttcac
     1141 gcacaagaac gggcgcccct acaagtgagc aaggtccaca tcaagagcgg ccgcgtgatc
     1201 gacccgaaaa accgcgtcga cgcgaagcag gacgtattca tagcggacgg aaaaatcgcc
     1261 gcgctcggca aggctccggc gggatttcgc gccgagcgca cgatagcggc ggatgcgctc
     1321 gtggtctgtc cgggcctgat cgacctctcc gcgcggctgc gcgaaccggg gttcgaatac
     1381 aaggcgacgc tggcctcgga aatgcaggca gcggtcgcag gcggcgtcac gagcctcgct
     1441 tgtccccccg acacggaccc gccgctcgat gaaccgggcc tcgtggaaat gctcaagtac
     1501 cgcgcgcaga gcctgaatct cgcacacgtc tatccgctcg gtgccctcac ctggaagctc
     1561 ggcggcgagc ggctcaccga gatggcggta ctgcgagacg cggggtgcgt cgcgttctcc
     1621 cacgccgaca cgccgcttcc ggacacgcag atgctgtggc gcgcgctgca atacgcagca
     1681 accttcggaa tcgcggtgtg gctgcggccg caggacgggt ttctcgcgcg cggcggtgtg
     1741 gcacacgacg gggaagtcgc cacccggctc ggcctgcagg ggattccggc gttcgcggag
     1801 acgatcgcgc tcgcgaccat cctgcagctc gtgcgcgcca cgcgcacccg ggtccatcta
     1861 tgccgccttt ccacggccga aggcgttgcg atgctgcgcg tggccaaaaa agagggcctg
     1921 cccgtcagct gcgacgcgag cgcccatcac gcgcacctct cggagatcga cctcggctac
     1981 ttcgattcac actgcaacgt ggtcccgccg ttccggtcct tgcgcgaccg cgatgcgcta
     2041 cgcgcgggcc tcgccgacgg gacgatcgat gcgctgtgct cggatcacgc gccggttgac
     2101 gaggacgcga agcagctgcc tttcgccgaa tcggaac
//
LOCUS       DASPZD010000115         4413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_309120, whole genome shotgun sequence.
ACCESSION   DASPZD010000115 DASPZD010000000
VERSION     DASPZD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309120"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..858
                     /gene="rplB"
                     /locus_tag="VEM33_02145"
     CDS             19..858
                     /gene="rplB"
                     /locus_tag="VEM33_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HYS50659.1"
                     /translation="MPLVKFKPTSPGRRQLVKFVNPDLHKGGPYQPLVEKQSKKAGRN
                     VNGHITVRHQGGGHKQNYRIVDFRRNKDGIAAKVERLEYDPNRSANIALLCYADGERR
                     YIIAPKGVTVGTQLLSGAEAPIKPGNTLPLRNIPVGTTVHCIEMQPGGGAQLARAAGA
                     SVQLLAREGSYAQLRLRSGEIRKVHVDCRATIGEVGNEEHSLETIGKAGRVRWRGVRP
                     TVRGVAMNPIDHPHGGGEGKTAAGQDPVSPWGVLTKGYKTRKNKRTRGMIIQFRHKKR
                     DKG"
     gene            862..1128
                     /gene="rpsS"
                     /locus_tag="VEM33_02150"
                     /pseudo
     CDS             862..1128
                     /gene="rpsS"
                     /locus_tag="VEM33_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011137729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            1276..1644
                     /gene="rplV"
                     /locus_tag="VEM33_02155"
     CDS             1276..1644
                     /gene="rplV"
                     /locus_tag="VEM33_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HYS50660.1"
                     /translation="METRATLYGVRLSAQKGRLVADQVRGLPVEKALNLLAFSPKKGA
                     HIIKKVLESAIANAEHNDGADVDELTVKTILVEQAMSLKRFQARAKGRGNRITKRTCH
                     IFVTVGDDEKKKAPAKPAKK"
     gene            1652..2581
                     /gene="rpsC"
                     /locus_tag="VEM33_02160"
     CDS             1652..2581
                     /gene="rpsC"
                     /locus_tag="VEM33_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HYS50661.1"
                     /translation="MGQKIHPIGFRLSVNKNWSSRWYANSKNFATMLNEDLKVRDYLK
                     KRLSHASVGKVMIERPAKDARITIHSARPGVVIGKKGEDIEMLKADLRKLLGVQMVHV
                     NIEEIRKPEVDAQLIADSIAQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSAGRLNG
                     IEIARTEWYREGRVPLHTLRADIDYGFSEAKTTYGVIGIKCWVYKGEVMAKGDQPVAP
                     PAPTADEVLAAKKVKKAAPKPVTSALEDKPEPIAATKTAPDAGTSAAAKPKVARPHPV
                     AAKKAVSEAKGKAEAKSEAKPKTAVKKSRKPKA"
     gene            2592..3005
                     /gene="rplP"
                     /locus_tag="VEM33_02165"
     CDS             2592..3005
                     /gene="rplP"
                     /locus_tag="VEM33_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HYS50662.1"
                     /translation="MLQPARTKYRKQQKGRNRGVATRGTKVSFGEYGLKATTRGRLTA
                     RQIEAARRAMTRHIKRGGRIFIRIFPDKPVSQKPAEVRMGNGKGNPEYFVAEIRPGKV
                     LYEMDGVDEPTAREAFALASAKLPLRTIFVFRQMG"
     gene            3029..3223
                     /gene="rpmC"
                     /locus_tag="VEM33_02170"
     CDS             3029..3223
                     /gene="rpmC"
                     /locus_tag="VEM33_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HYS50663.1"
                     /translation="MKASELRAKDPAQLSKELEELLRAQFGLRMQKATQQLTNSSQLK
                     NVRRDIARVRTILKQKAEVK"
     gene            3220..3483
                     /gene="rpsQ"
                     /locus_tag="VEM33_02175"
     CDS             3220..3483
                     /gene="rpsQ"
                     /locus_tag="VEM33_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HYS50664.1"
                     /translation="MSETRTTRTLTGRVVSDKMDKTVTVLIERRVTHPMYGKIVTRSK
                     KYHAHDEKNECGEGDLVVIEECRPISRTKAWRIAKLVEKARAA"
     gene            3641..4009
                     /gene="rplN"
                     /locus_tag="VEM33_02180"
     CDS             3641..4009
                     /gene="rplN"
                     /locus_tag="VEM33_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HYS50665.1"
                     /translation="MIQMQTVLDVADNTGARAVMCIKVLGGSKRRYANIGDVIRVSIK
                     DAAPRGRVKKGEIYHAVVVRTAFGVRRADGSRIRFDSNAAVLLTPKLEPIGTRIFGPV
                     TRELRSERFMKIVSLAPEVL"
     gene            4039..4356
                     /gene="rplX"
                     /locus_tag="VEM33_02185"
     CDS             4039..4356
                     /gene="rplX"
                     /locus_tag="VEM33_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HYS50666.1"
                     /translation="MEKIKKGDDVIVTAGKDKGKRGTVLRIVDARHLVVEGVNRVKKH
                     QRPNPVKGQTGGIVDKDMPIDVSNVALYNPQTKKADRVGFKQMEDGRKVRVFKSNGEM
                     VDA"
BASE COUNT         1014 a         1330 c         1436 g          633 t
ORIGIN      
        1 caggcccagg agtagaccgt gccgctcgta aaattcaaac ccacctcgcc cggccggcga
       61 caactcgtca agttcgtaaa tccggatctg cacaagggcg ggccttatca gcccctggtc
      121 gagaagcaat ccaagaaggc cggccgcaac gtcaatgggc acatcacggt gcgccaccaa
      181 ggcggcgggc acaagcagaa ttaccgcatc gtcgattttc gccgcaacaa ggacggtatt
      241 gcggccaagg tcgagcggct cgagtacgac ccgaaccgga gcgcgaacat cgccttgctc
      301 tgctatgccg acggcgagcg ccgctacatc attgcgccca agggtgtgac agtcggcacg
      361 cagcttctgt ccggggccga ggcgccgatc aaaccgggga ataccctgcc gcttcggaat
      421 attccggtcg gcaccacggt tcactgcatc gagatgcagc ccggcggggg cgcacagctc
      481 gcgcgcgccg cgggcgcctc ggttcagctg cttgcgcgcg agggcagcta cgcgcagttg
      541 cggctgcgtt cgggtgagat ccgcaaggtg cacgtcgact gccgcgccac catcggcgaa
      601 gtcggcaacg aggagcatag cctcgagacg atcggcaagg cgggacgcgt ccgctggcgc
      661 ggcgtgcgcc cgaccgtgcg cggcgtcgcg atgaatccaa tcgaccaccc gcatggcggc
      721 ggcgagggca agaccgcggc aggacaggat ccggtaagcc cctggggggt gctgaccaag
      781 ggctacaaga cccgcaagaa caagcgcaca cgcggcatga tcatccaatt ccgccacaag
      841 aaacgagaca agggttagac catgggacgc tcggtcaaga agggtccctt cgtggaccac
      901 catctggtga agaaggtcgg tgcggcgcgc gcggcgagcg acaagcgccc gatcaagacc
      961 tggtcgcgcc gctccacgat cctgccggat ttcgtcgggc tgaccatcgc ggtccacaac
     1021 gggaagcagc acgtcccggt atacatctcg gaaaacatgg tggggcacaa gctgggtgaa
     1081 ttcgctctga ctcgcatttt caaaagccac ggcatggcgg acaagaagct tctcgaagct
     1141 gctgcggccg cgagagcggg cggaggaggg cccggggcgc cgggagctcc gacagcgccg
     1201 ggggcgcctg cgccaggcgc cgccccggca cctgccgccc ccgcggcggg cggcgagaag
     1261 aagtaaggag aagacatgga aacccgcgcg acgctgtacg gggtgcggct ttcggcccag
     1321 aaggggcggc tcgtcgccga ccaggtccgc gggctccccg tggaaaaagc cctcaatctg
     1381 ctcgccttca gtccgaagaa gggggcgcac atcatcaaga aggtgctcga atcggcgatc
     1441 gcgaacgccg agcacaacga cggcgccgac gtcgacgagc tgacggtgaa gaccatcctc
     1501 gtggaacagg ccatgtcgct caagcgcttc caggcgcggg cgaagggccg gggcaaccgc
     1561 atcaccaagc ggacctgcca tattttcgtg acggtcgggg acgacgagaa gaagaaggcg
     1621 ccggccaagc cagcaaagaa ataggacacc catgggacag aaaattcatc cgatcggctt
     1681 caggctcagt gtcaacaaga actggtcctc gcgctggtac gccaacagca agaacttcgc
     1741 cacgatgctg aacgaggacc tgaaggtccg cgactatctg aagaaacggc tttcccacgc
     1801 ctcggtcggc aaggtgatga tcgagcggcc cgccaaggac gctcgcatca ccatccatag
     1861 cgcgcggccc ggcgtcgtga tcggcaagaa gggcgaagac atcgaaatgc tcaaggccga
     1921 tctgcgcaaa ctcctcggcg tgcagatggt ccacgtgaac atcgaggaaa tccgcaagcc
     1981 cgaggtcgac gcacagctca tagcggattc gattgctcag cagctggaaa aacggatcat
     2041 gttccgccgc gcaatgaagc gcgctatgca aaacgcgatg cgcctgggcg ctcaggggat
     2101 caagataatg agcgcggggc gcttgaacgg catcgagatc gcccgcacag agtggtatcg
     2161 cgaggggcgt gttccccttc atacgctgcg ggcggacatc gactacggct tctcggaggc
     2221 gaagaccacc tacggcgtga tcggcatcaa gtgctgggta tacaaagggg aagtcatggc
     2281 caagggcgac cagccggtcg cgcctcctgc tccgaccgcc gacgaggtcc tagcggcgaa
     2341 gaaagtgaag aaggcggcgc ccaaacccgt gactagcgct ctcgaagaca agcccgaacc
     2401 gatcgccgcg accaagacgg cgcccgacgc ggggacctcc gccgcggcca agcccaaggt
     2461 tgcccggccc catccggtag ccgcgaaaaa ggccgtgagc gaagcgaagg ggaaggccga
     2521 agcgaagtcg gaagcgaagc cgaaaaccgc ggtgaagaaa tccagaaagc ccaaggctta
     2581 ggagcaaatt catgcttcaa ccggctcgaa cgaaataccg caagcaacag aagggacgca
     2641 acaggggcgt cgctacgcgg gggacgaagg tttccttcgg tgaatacggc ctgaaggcga
     2701 cgacgcgcgg acgccttacc gcgcgccaga tcgaggccgc acgccgtgcc atgacgcgcc
     2761 acatcaagcg gggcgggcgc atcttcatcc gcatctttcc ggacaaaccc gtttcgcaaa
     2821 aacccgcaga ggtgcgaatg ggcaacggca agggcaaccc ggagtatttc gtcgccgaaa
     2881 tcaggccggg caaggtgcta tacgaaatgg acggggtgga cgagccgacg gcgcgggaag
     2941 cctttgccct cgcctcggcc aagcttcccc tgcgcacaat tttcgtgttc cgtcagatgg
     3001 ggtgagggtc ggagaagagg acatgatcat gaaagccagt gaactccgcg ccaaggatcc
     3061 ggcgcagctt tccaaggagc tcgaggagct gctgcgcgcc cagttcggtc tgcgcatgca
     3121 gaaagcgacg cagcagctca ccaacagcag ccaactcaaa aacgtgcgcc gtgacatcgc
     3181 acgggtgcgc accatcctga agcaaaaggc ggaagtgaaa tgagcgaaac caggaccacc
     3241 cgtacgctga ccggacgagt cgtcagcgac aagatggaca agaccgtcac ggtattgatc
     3301 gagcgccgcg tcacccatcc gatgtacgga aagatcgtga cccgctcgaa gaaataccac
     3361 gcccacgacg aaaagaacga gtgcggtgag ggtgatttgg tcgtgatcga ggagtgtcgc
     3421 ccgatctcca ggaccaaagc gtggcggatc gccaagctgg tcgaaaaggc acgggccgcg
     3481 taggcttcgc gcgctgcgaa gcgaaggctg atctcccggt agatttggct tggcaagggt
     3541 cttgatttcg cgtagaattc gctgcctttt cacgtcccgg acgggatcca aaactgcctg
     3601 cctgtaatgc attcgggtcg gacaagttgg agacggaaaa atgattcaga tgcagacggt
     3661 gctcgatgtc gccgataaca ccggcgcgcg cgctgtgatg tgtatcaagg tgctgggcgg
     3721 ctcgaaacgc cgctacgcca acattggcga cgtcatcagg gtgagcatca aggacgcggc
     3781 gccgcgcggc cgcgtcaaga aaggcgagat ttaccacgcc gtggtggtgc gcacggcctt
     3841 cggcgtgcgc cgcgccgacg ggagccgcat ccggtttgac tcgaacgcgg cggtgctcct
     3901 tacacccaag ttggagccga tcggcacgcg catcttcggg ccggtgaccc gggagcttcg
     3961 cagcgagcgc ttcatgaaga tcgtttcgct cgcgcccgag gtgctgtgat cgcttacgac
     4021 gactgatgcc ctgagactat ggaaaaaatc aaaaaaggcg acgacgtcat cgtcaccgcc
     4081 ggcaaggaca agggaaagcg ggggacggtg ctgcggatcg tggatgcaag gcacctcgtc
     4141 gtggagggag tcaaccgcgt gaagaaacac cagcgcccta accccgtgaa gggtcagacg
     4201 ggcggaatcg tggacaagga catgcccatc gacgtctcga acgtcgctct gtacaacccg
     4261 cagaccaaga aggccgaccg cgtagggttc aagcagatgg aggacgggcg caaggtgcgc
     4321 gtgttcaagt ccaacggtga aatggtggac gcttgatccc ctaggatttc gaaaaaccgt
     4381 ttatggcccg cctacaagac tactaccgca aag
//
LOCUS       DASPZD010000116         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1278067, whole genome shotgun sequence.
ACCESSION   DASPZD010000116 DASPZD010000000
VERSION     DASPZD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278067"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VEM33_02190"
     CDS             <1..279
                     /locus_tag="VEM33_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50667.1"
                     /translation="LAVLDALIDSWDIDLAAFSLEIVLRRRVGAGRVKRFEFVEREAG
                     AEPGLKQFLADPALSGTATPQELEFLRNLRFKGRRPTAPKSTTGWTGS"
     gene            264..845
                     /locus_tag="VEM33_02195"
     CDS             264..845
                     /locus_tag="VEM33_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50668.1"
                     /translation="MDRFLKLPDGELARVAELARREELKRELGDTPEPLFPRLRDLIL
                     RKCRPEKAKQILAIFERQPFGELERLVAKQLLDVAGLATDIFHLSARHLAVLDALIDS
                     WDIDLAAFSLEIVLRRRVVAGRVKRFEFVEREAGAEPGLKQFLADPALSATATPQELE
                     FLRNLRFKGRRPTALYYYRELQNLRDPLHFRNA"
     gene            1037..1273
                     /locus_tag="VEM33_02200"
     CDS             1037..1273
                     /locus_tag="VEM33_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50669.1"
                     /translation="MEAIRDELTARARREYVQYTMLAILSAALGRLDEAFELLEHACD
                     DRDGILVYSRRYPFFSAFQHDPRMERIYRRIGLP"
     gene            complement(1305..1520)
                     /locus_tag="VEM33_02205"
     CDS             complement(1305..1520)
                     /locus_tag="VEM33_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50670.1"
                     /translation="MSEIHCHTCGGFIIDPAGVSYRVPSPATVPAAQRSGLCTCHPSI
                     VYGPPPGYLSLPGLPPSIDLRAMAARN"
     gene            complement(1595..1909)
                     /locus_tag="VEM33_02210"
     CDS             complement(1595..1909)
                     /locus_tag="VEM33_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50671.1"
                     /translation="MYARNVTLHLKANSAPEFTRTLETDILPVLRKQNGFKDEITFVT
                     ENGKEALAISLWEKKENAEAYSRDTYPTVLQSLAKVVDGTPRVEAYEVSNSTFHKIAS
                     KN"
BASE COUNT          362 a          700 c          712 g          396 t
ORIGIN      
        1 ctcgcggtgc tcgatgcgct gatcgactcg tgggacatcg atctcgccgc cttcagcctc
       61 gagatagtgc tgcggcgacg ggtgggggcc gggcgggtga aacggttcga gttcgtggag
      121 cgcgaggctg gggcggagcc gggactcaag caattcctgg ccgatcccgc gttgagcggc
      181 acggcgacgc cgcaggagct cgagttcttg cgaaacctcc gcttcaaagg ccggcgccca
      241 accgcaccga aatctacgac cggatggaca ggttcctgaa gctgccggac ggcgagctcg
      301 cgcgggtggc ggagctggcc cgcagagaag agctgaagcg ggagctgggc gacacgcccg
      361 aacccctgtt ccctcggctg cgcgacttga tcttgcgcaa gtgccgtccc gagaaggcca
      421 agcagatcct cgcgatcttc gagcgtcagc ccttcggcga gctcgagcgc ctcgtcgcga
      481 agcagctgct cgacgtcgcc ggcctcgcga ccgatatctt ccacctgtca gccaggcacc
      541 tcgcggtgct cgatgcgctg atcgactcgt gggacatcga tctcgccgcc ttcagcctcg
      601 agatagtgct gcggcgacgg gtggtggccg ggcgggtgaa acggttcgag ttcgtggagc
      661 gcgaggctgg ggcggagccg ggactcaagc aattcctggc cgatcccgcg ttgagcgcca
      721 cggcgacgcc gcaggagctc gagttcttgc gaaacctccg cttcaaaggc cggcgcccaa
      781 ccgccctcta ctattaccgc gagctgcaga acttgaggga cccgctgcac tttcgaaacg
      841 cgtagtcgac ttgcctcccg ggccccggcc ggtctatgct aggcgcccgt gcaatcgacg
      901 accgtctccc gctggcgcgc cttgctgggc gatgcccact tctggatccc agtcgccgtg
      961 ctcatagccg gcctcgccat ccctggacct gtgcgcactc ggggtgtgct acagctccgc
     1021 cgggaaggtg gcagaggtgg aagcgattcg cgacgagctg accgcgcggg cgcgccgcga
     1081 gtacgtgcaa tacaccatgc tcgcgatctt gagcgccgcc ctcgggcgcc tcgacgaagc
     1141 cttcgagctg ctggagcacg cgtgcgacga ccgtgacggc atcctcgtct actcgcgccg
     1201 ctacccgttc ttcagcgcgt tccagcacga tccccggatg gagcggatct atcgccggat
     1261 cggactccct taacgcgcgc cacgacctcg cgcacgatgc ggttctagtt gcgcgccgcc
     1321 atcgcgcgga gatcgatgct cggcggcaac cccggcaacg acaagtaacc cggcggcggc
     1381 ccgtacacga tcgacggatg acaggtgcaa agcccgctac gctgcgcggc cgggaccgtc
     1441 gcgggggacg gcacccggta ggaaacgccc gccgggtcga tgatgaaccc gccgcacgtg
     1501 tgacaatgga tctcgctcat ctgtcgactc cttccgaaaa aagacgaggg gcggccgggt
     1561 gccgcccctc gctatcgtga caaagaccgg cgccctagtt cttcgacgcg atcttgtgga
     1621 acgtcgagtt ggagacctcg taggcctcga ctcggggcgt gccatcgacg accttcgcga
     1681 ggctctgcag caccgtggga taggtgtcgc ggctgtaagc ctccgcgttc tccttcttct
     1741 cccacaagct gatggccagc gcttccttgc cgttctcggt gacgaacgtg atctcgtcct
     1801 tgaagccgtt ctgcttgcgc agcaccggca ggatgtccgt ttcgagcgtc cgggtgaact
     1861 cgggggccga attggccttc aggtgcagcg tgacgttgcg tgcgtacatg agatgactcc
     1921 tcgacgactg cgacatgagc ttcacgtctg ctgaacactt ccaagctggt gcaacctagc
     1981 cggatgtact atgtttgtca agtactgcgc tactcagagt aatcaactta atcgttgcag
     2041 ttaatagcgt ggccgtgaca ggggatggtc ctgtggacgt ggcgttcgta atccgggagc
     2101 ggttggtgga attgggtttg gagcagcgcg acctcgcccg tgcggcccgg gtgacggagt
     2161 catacgtctc
//
LOCUS       DASPZD010000117         6058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_308252, whole genome shotgun sequence.
ACCESSION   DASPZD010000117 DASPZD010000000
VERSION     DASPZD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308252"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /gene="tuf"
                     /locus_tag="VEM33_02215"
     CDS             <1..289
                     /gene="tuf"
                     /locus_tag="VEM33_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898355.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HYS50672.1"
                     /translation="TPHTKFECEVYVLSKEEGGRHTPFFPGYRPQFYFRTTDVTGSCE
                     LPKGTDMVMPGDNVKMVVTLIAPIAMEQGLRFAIREGGKTVGAGVVAKVIE"
     gene            301..376
                     /locus_tag="VEM33_02220"
     tRNA            301..376
                     /locus_tag="VEM33_02220"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:334..336,aa:Trp,seq:cca)
     gene            424..774
                     /gene="secE"
                     /locus_tag="VEM33_02225"
     CDS             424..774
                     /gene="secE"
                     /locus_tag="VEM33_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HYS50673.1"
                     /translation="MADKIKVAIAVLLLIAGIAGFYFLSESPMVLRVASVLAGIAAGS
                     VVGWTTEPGKRFFVFAQEAVVETKKVVWPTRKESLQTTGAVFAFVVAMAVFLWLTDKS
                     LEYVMYDLLLGWKK"
     gene            777..1325
                     /gene="nusG"
                     /locus_tag="VEM33_02230"
     CDS             777..1325
                     /gene="nusG"
                     /locus_tag="VEM33_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HYS50674.1"
                     /translation="MTANATKRWYVVHAYSGFEKSVQRTLLDRIQRAGMQEKFGKILV
                     PTEEVVEMKSGQKNISERKFFPGYVLVEMDMTDDAWHLVKNTPKVTGFVGGAHGRKPP
                     PISEKEVKNILQQMQEGVEKPKPKVLFEIGEAVRVREGPFTDFHGTVEDVNYDKSKLR
                     VSVTIFGRATPVELEFGQVEKA"
     gene            1377..1811
                     /gene="rplK"
                     /locus_tag="VEM33_02235"
     CDS             1377..1811
                     /gene="rplK"
                     /locus_tag="VEM33_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HYS50675.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKTEPGLMLPVVITAYQDKSFSFIIKTPPATVLIKKAAGIESGSKTPHTDKVGKIT
                     RAQAEEIAKAKMPDLTAADLDAAVRTIAGSARSMGVIVEGVK"
     gene            1808..2521
                     /gene="rplA"
                     /locus_tag="VEM33_02240"
     CDS             1808..2521
                     /gene="rplA"
                     /locus_tag="VEM33_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HYS50676.1"
                     /translation="MSKLPKRKQTWAKLREHNRNYPVLDALKIIKQAAAAKFDEAVDV
                     AINLGIDAKKSDQTVRGSVVLPAGTGKKIRIAVFAQGDKAQAAKDAGADIVGMEDLAE
                     SVKAGKLDFDVVIASPDTMRVVGQLGQILGPRGLMPNPKVGTVTPDVAAAVKNARAGQ
                     VQYRADKSGIVQCSIGRASFTPEQLRDNLVALVDAVNKSKPSNVKGIYLRKVSVSSTM
                     GPGVRVDVGSFGGGATAQH"
     gene            2748..3281
                     /gene="rplJ"
                     /locus_tag="VEM33_02245"
     CDS             2748..3281
                     /gene="rplJ"
                     /locus_tag="VEM33_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HYS50677.1"
                     /translation="MGLDLDKKKAMVAEVSAQLAKAKTVIVAEYRGLEVGRMTELRAK
                     ARKSGVYLRVLKNTLARRAVEGTPFAGLSQHMKGPLVYGISPDPVATAKVLNEFAKAN
                     DKLVIRAGAMPNAVISAKEVTALALMPSREELLAKLLGTMQAPIVKFVRTLNEVPARF
                     ARTLAAVRDKRQAAGAQ"
     gene            3326..3709
                     /gene="rplL"
                     /locus_tag="VEM33_02250"
     CDS             3326..3709
                     /gene="rplL"
                     /locus_tag="VEM33_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYS50678.1"
                     /translation="MSVAKAEILDAISNMSVLELSNLIKDLEEKFGVSAAAATVAAAA
                     PAGGGGAAAAVEEKTEFTVMLTGFGENKVNVIKAVRELTGLGLKEAKDLVDGAPKPVK
                     EGVSKADSEAILKKLIDAGAKAEIK"
     gene            3897..>6058
                     /gene="rpoB"
                     /locus_tag="VEM33_02255"
     CDS             3897..>6058
                     /gene="rpoB"
                     /locus_tag="VEM33_02255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYS50679.1"
                     /translation="MTTAATYSSTEKKRIRKSFAKRASVLSVPFLLATQLESYAAFLQ
                     AAVLAEKRKNEGLQAAFTSIFPISSHSGNARLEFVSYSLGEPPFDVKECQQRGLTYAS
                     PLRAKVRLTIMDKEASKPTVKEVKEQEVYMGEIPLMTTTGSFIINGTERVIVSQLHRS
                     PGVFFEHDRGKTHSSGKLLFSARIIPYRGSWLDFEYDPKDYLYFRVDRRRKMPVTILL
                     KAIGMTPEAILAAFYDFDTFQLAKSGNQFELVPERLRGEVARFDFVDKAGKTIVQKDK
                     RITGKHIRDMEAAGMKRMAVPDEFLIGRTLAHNVINKESGEILANANDEITELLLAKL
                     AEAGIETIKTVYTNDLDQGGYISQTLKIDETADQSAARVAIYRMMRPGEPPTEDAVET
                     LFNGLFYSEDRYDLSAVGRMKFNRRAYPEKIEEKAPGWLRRFYARVGSQGAEGPGTLS
                     NDDILAVVGILVELRNGRGEIDDIDHLGNRRVRSVGELAENQFRAGLVRVERAVKERL
                     SQAESENLMPHDLINAKPISAAIKEFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGG
                     LTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLALYARTNRYGFLETPYRKVV
                     SGKITNEIDFLSAIEEGNFVIAQANAQIDKNGKLVDGLVSCRFKNEFTLATADRVQYM
                     DVAPSQIVSVAASLIPFLEHDDANRALMGSNMQRQAVP"
BASE COUNT         1275 a         1813 c         1985 g          985 t
ORIGIN      
        1 caccccgcac accaagttcg agtgcgaggt gtacgtgctc agcaaggagg aaggcgggcg
       61 gcatacgccg ttcttcccgg ggtaccggcc gcagttttac ttcaggacca ccgacgtgac
      121 gggcagctgc gagctgccca aagggaccga tatggtgatg ccgggggaca acgtgaagat
      181 ggtagtgacg ctgattgcgc ccattgcgat ggagcagggc ttgaggtttg ccatccgcga
      241 gggcggcaag accgtcggcg ccggcgtcgt cgcgaaggtt attgagtgaa ggcgggacac
      301 aggccaatag ctcaactggc agagcgtcgg tctccaaaac cgaaggttgg gggttcgatt
      361 ccctcttggc ctgccaagga acggacgggt agcgggactg cggtcccgct cgattagagg
      421 cggatggcgg acaaaatcaa ggttgcgata gcggtgctgc tgctgatcgc gggcatcgcg
      481 ggcttttatt tcctctcgga aagcccgatg gtgctgcgcg tggcgagcgt gcttgccggc
      541 atcgcggcgg gctccgtcgt gggatggacc accgagccgg gaaagcgctt cttcgtgttc
      601 gcccaggaag cggtggtcga aacgaaaaag gtggtgtggc cgacgcggaa ggaatcgctg
      661 cagaccaccg gcgcggtgtt cgcattcgtc gtggcgatgg cggtgttcct ctggctcacg
      721 gacaagagcc tcgaatacgt gatgtacgac ctgcttctcg gatggaaaaa gtagctatga
      781 ccgctaacgc aaccaagcgc tggtacgtgg tgcacgccta ctccgggttc gagaagtccg
      841 tgcagcgcac gcttctggac cgtattcagc gcgccggcat gcaggaaaaa ttcggcaaga
      901 tcctggtgcc gaccgaggaa gtcgtcgaga tgaagagcgg gcagaagaac atctcggagc
      961 gcaagttctt cccagggtac gtgctcgtgg aaatggacat gaccgacgat gcctggcatc
     1021 tggtgaagaa tacgcccaag gtcaccggtt tcgtcggcgg ggcgcacggc aggaagccgc
     1081 cgccaatcag cgagaaggaa gtgaagaaca tcctccagca gatgcaggag ggtgtggaaa
     1141 agcccaagcc caaggtgctg ttcgagatcg gcgaggcggt acgggtcagg gaaggcccgt
     1201 tcaccgattt tcacggcacg gtcgaagacg tgaattacga caagtcgaag ctgcgcgtgt
     1261 cggtgacgat cttcgggcgg gcgacaccgg tggaattgga attcgggcag gtcgagaagg
     1321 cgtaacaacc ggggcggacg cggaaagcat ccgcttgcac ccaccaggag aaatgcatgg
     1381 caaagaaaat tgtcggattc atcaagctgc aggtgccggc gggcaaggcc aatcccagcc
     1441 cgcccatcgg gccggcgctc ggccagcggg gcctgaacat catggaattc tgcaaggcgt
     1501 tcaacgcgca gacgcagaag accgagcccg gcctgatgct gccggtggtc atcaccgcct
     1561 accaggacaa gagcttcagc ttcataatca agactccgcc cgcgaccgtc ctaatcaaga
     1621 aggccgcggg aatcgaatcg gggagcaaga ccccgcacac cgacaaggtg gggaagatca
     1681 cccgcgccca ggccgaggag atcgcgaaag ccaagatgcc cgacctgacc gccgccgacc
     1741 tcgacgcggc ggtacgcacg atcgcgggca gcgcgcgctc gatgggcgtg atcgtggaag
     1801 gcgtgaaatg agcaagctcc cgaaacggaa gcaaacctgg gcgaagctgc gcgagcacaa
     1861 cagaaactac cccgtgctcg acgcgttgaa gatcatcaag caggcggcgg ctgccaagtt
     1921 cgacgaagcc gtcgacgtgg cgatcaatct gggcatcgat gcgaagaagt ccgaccagac
     1981 ggtgcgcgga tcggtagtcc tccccgccgg aacgggcaag aagatcagga tcgccgtgtt
     2041 tgcacagggc gacaaggccc aggcggcgaa ggacgcgggc gcggacatcg tcggcatgga
     2101 agatctcgcc gaaagtgtca aggcggggaa gctcgatttt gacgtcgtga tcgcgagccc
     2161 cgacacgatg cgcgtggtcg ggcaactggg ccagatcctg gggcctcgcg ggctgatgcc
     2221 caacccgaag gtggggaccg tgacgcccga tgtcgcggca gcggtaaaaa atgcgcgcgc
     2281 cggccaggtg cagtaccgag ccgacaagtc cggcatcgtg caatgctcca tcgggcgcgc
     2341 gtctttcacc ccggagcagc tgcgcgacaa cctcgtcgcg ctggtcgatg cggtcaacaa
     2401 gtcgaagcct tccaacgtga agggaatcta cctgcgcaag gtgtcggtct cttcgaccat
     2461 ggggccgggg gtgcgggtgg acgtggggag ctttggcggc ggcgcgacgg cgcagcattg
     2521 atatggatga cggaactttg ggccatcgca cggctcgcac ggcgtgcggt gggttatcaa
     2581 agaccgtagg aaccgagagg tttaatcggc gggaacgaag tgcagtcgaa caatcctgcc
     2641 gtcctacgca gatggcgagc cccatgcagg atttccctga atgcaaggtc gccgtgggtg
     2701 ccgcaagtct agacgacggg cggcttttga caaattggag gtagaccttg ggtctcgatc
     2761 ttgacaagaa gaaggcaatg gtcgccgaag tctccgcgca gttggcgaag gcgaagacgg
     2821 tcatcgtcgc agagtaccgt ggtctggaag tgggccgcat gaccgagctg cgcgcgaagg
     2881 cgcgcaagtc gggcgtgtac ctgcgcgtcc tcaagaacac ccttgcgcgc cgcgccgtcg
     2941 aaggcacgcc gttcgccggg ttgtcccagc acatgaaggg accgctcgtc tacggcatct
     3001 cgcccgaccc ggtggcgacc gcgaaggtgc tcaacgagtt cgccaaagcg aacgataagc
     3061 tcgtcatccg cgccggtgcc atgccgaacg ccgtgatttc cgccaaggaa gtcaccgcgc
     3121 tcgcgctgat gccgtcgcgc gaagagctgc tcgcgaagct tttgggcacg atgcaggccc
     3181 cgatcgtcaa gtttgtaagg acgctgaacg aggtgccggc gcgctttgcg cgcaccctcg
     3241 ccgcggtccg cgacaagcga caggcggccg gcgcgcagta gcgcgccttc cctaccggtt
     3301 agaacactga ttcaggagaa acgaaatgtc tgtagcaaaa gcagagattc tggatgcgat
     3361 ttccaacatg tcggtgttgg agctttccaa tctgatcaag gacctcgaag agaagttcgg
     3421 cgtgtccgcc gccgcggcga cggtggcggc agccgctccg gcgggcggcg gcggcgctgc
     3481 ggccgcggtg gaggagaaga ccgagtttac ggtcatgctc accggtttcg gcgaaaacaa
     3541 ggtcaacgtg atcaaagcgg ttcgcgagct caccgggctc ggtctgaagg aagccaagga
     3601 cctggtggac ggagcgccca agcccgtgaa ggagggtgtg tccaaagcgg actcggaagc
     3661 gatcttgaag aagttgatcg acgccggcgc caaggccgag atcaagtaac cacggcccaa
     3721 acgatcttgc accacagagc gcacagagag cacggagagt tgttagggtt ttcctccgtg
     3781 cactccgtga tctctgaggt tcaggagggt tcactgcgca gagcgcaggt gagagggggc
     3841 ggaactttcc gcgccgcccc ctgtcccctt ccctctggct tcgagacgga gcgtccatga
     3901 ccaccgccgc aacctattca tctaccgaga aaaaacgcat ccgcaagagc ttcgcgaagc
     3961 gtgcgagcgt gctcagcgtt ccgtttctgc tggcgaccca gctcgagtct tacgcggcct
     4021 tcctgcaggc ggcagtcctt gcggaaaagc gcaagaacga gggcttgcag gcggcgttca
     4081 cctcgatctt ccccatctcc agccactcgg ggaacgcgcg gctcgaattc gtcagctact
     4141 cgctcggcga gccgcccttc gacgtcaagg aatgccagca gcgcgggctg acctacgcga
     4201 gcccgctccg cgccaaggtg cgcctcacca tcatggacaa ggaagcctcc aagcccacgg
     4261 tgaaagaggt caaggagcag gaagtgtaca tgggcgagat tccgctgatg accaccacgg
     4321 gctcgttcat catcaacggc accgagcgcg tgatcgtcag ccagctgcac cgctccccgg
     4381 gcgtgttctt cgaacacgat cgcggcaaga cgcactcctc gggcaagctg ctcttctccg
     4441 cgcgcatcat cccgtaccgc ggctcgtggc tcgacttcga atacgacccc aaggactatc
     4501 tctatttccg cgtcgaccgc cgccgcaaga tgccggtgac gatcctgctc aaggcgatcg
     4561 gcatgacgcc cgaggccatc ctcgcggcgt tctacgattt cgacaccttc cagctcgcca
     4621 agagcggcaa ccagttcgaa ctggtgcccg agcgcctgcg cggcgaggtc gcgcgcttcg
     4681 actttgtcga caaggcgggc aagacgatcg tgcagaagga caagcgcatc accggcaagc
     4741 acatccgcga catggaagcc gctggaatga agcggatggc ggtaccggac gagttcctga
     4801 tcggccgtac gctcgcgcac aacgtcatca acaaggagtc cggcgagata ctcgccaacg
     4861 ccaacgacga aatcaccgaa ctcctgcttg cgaagctcgc cgaagcgggt atcgagacga
     4921 tcaagaccgt ctacaccaac gacctcgacc agggcggtta catctcgcag acgctgaaga
     4981 tcgacgagac tgccgaccaa agcgcggcgc gcgtcgccat ctatcgcatg atgcgtccgg
     5041 gcgagccgcc cacggaagat gcggtggaga ccctgttcaa cgggctgttc tactcggagg
     5101 accgctacga cctttctgcg gtcggccgca tgaagttcaa ccgccgcgcc tacccggaga
     5161 aaatcgagga aaaggccccc ggctggctgc gccggttcta tgcccgggtc ggctcgcagg
     5221 gagccgaagg cccggggacg ctgtcgaacg acgatattct cgcggtggtg gggatcctcg
     5281 tggagctgcg caacgggcgc ggggagatcg acgacatcga tcatctcggc aaccggcgcg
     5341 tgcgctcggt gggcgagctc gccgagaacc agttccgcgc gggcctggtt cgcgtcgagc
     5401 gagcggtgaa agaacgcctc tcgcaagcgg agtccgagaa tctcatgccc cacgacttga
     5461 tcaacgccaa gccgatctcg gcggcgatca aggagttttt cggttcgagt cagctgtcgc
     5521 agttcatgga ccagacgaac ccgctttcgg aaatcacgca caagcggcgc gtatcggcat
     5581 tgggaccggg gggccttacc cgtgagcgcg cgggcttcga ggttcgcgac gtgcacccga
     5641 ctcactatgg ccgggtgtgc cccatcgaaa cgccagaagg cccgaacatc ggtcttatca
     5701 actcgctcgc gctctacgcc cgcaccaacc ggtacgggtt cctggagacg ccgtacagga
     5761 aggtcgtgag cggaaaaatc accaacgaga tcgatttcct ctcggcaatc gaggaaggca
     5821 acttcgtcat tgcgcaggcg aatgcgcaaa tcgacaagaa cggcaagctc gtcgatggcc
     5881 tggtttcgtg tcgcttcaag aacgagttca cgctggccac cgcagaccgc gtccagtaca
     5941 tggacgtggc cccctcgcag atcgtttccg tggcggcctc gctgattccg ttcctcgagc
     6001 acgacgacgc gaaccgcgcc ctaatgggct cgaacatgca gcgccaggca gtgccctg
//
LOCUS       DASPZD010000118         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_911392, whole genome shotgun sequence.
ACCESSION   DASPZD010000118 DASPZD010000000
VERSION     DASPZD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911392"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VEM33_02260"
     CDS             complement(<1..370)
                     /locus_tag="VEM33_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYS50680.1"
                     /translation="MKKSTFPTPQDAEAAFYEALEAGELDAMMEVWAEDEEIVCVHPG
                     GARLAGYDQVRESWAQIFASGQRLKVHISNQVVLSGMMLAVHSVHENILVQGEPRSRP
                     PVAVTNVYLRTGNGWRMILHH"
     gene            complement(458..1792)
                     /locus_tag="VEM33_02265"
     CDS             complement(458..1792)
                     /locus_tag="VEM33_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYS50681.1"
                     /translation="MPLRSPAAKPASPEDLKGTVQALSDGTLAARAYCESCLARIRAT
                     DPRLKAWIALDDERALTIAAARDAERSQGAHTGALHGVPVGVKDLYDTDDLPTEMGSP
                     AFVGNQPGKNAELVGRILAAGGYVLGKTVTTEFAYMHPAETRNPWNPRHTPGGSSSGS
                     AAAVAARHVPVAVGTQTNGSVIRPAAFCGVVGFKPSLDSLPIQGALPFAETLDHVGLF
                     ARTVADAAYFAASLAESGTFAPEIEALSRPPKIAVLPRFPWNAAEPGAARHLQASLKR
                     LADAGTELKTVALPEDFDEAHRVHRTIMLYEGAREHATRQASYRRVMSAVLNAAIDEG
                     LAMSHDDYRGALVRRAALAELALDLFEDCDALASLPAPGAAPERIDVTGDPSFCTLWT
                     LLGFPALTLPTGLSDGGMPYGMQLAGPAGGDDRLLRVARWCEAVIGFKDSPR"
     gene            complement(1809..2798)
                     /locus_tag="VEM33_02270"
     CDS             complement(1809..2798)
                     /locus_tag="VEM33_02270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HYS50682.1"
                     /translation="MKPKILVTRATFDDVIAKLRERFEVEDNQKDDAPWSAEEFRRRL
                     ADKDGVLPTGSDRVDAATLDAAPRLKAVCNIAVGYNNIDLAACTARGILATNTPGVLD
                     DTTADMTWALLMAAARRVTEAERWLRAGNWKGWKNDQFLGVDVHHATLGVIGMGRIGQ
                     AIARRAKGFSMRVLYHNRRRVPKAVEKQLGAKYVSMEKLLKESDFVSLNMPYSPQVHH
                     LVGARELALMKPTAVIVNAARGGIIDDAALVQALKERRIAAAGLDVFEGEPKFNPGFL
                     EVDNVALVPHIGSATRATRTAMAMLASRNLGAALSGRRPPNLLNPEAWPKRRR"
     gene            complement(2926..>3984)
                     /locus_tag="VEM33_02275"
     CDS             complement(2926..>3984)
                     /locus_tag="VEM33_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HYS50683.1"
                     /translation="RAATAPEIEASTAGLIEAGGLIAAPEQEREVSTGLIGAQEPEES
                     GVKEPAAESSKAPAAPQAGAEAAKGPPTAESERLPVPIWLWITFAVGIAIIVLIYWLW
                     SWERLEVFKMLLTSFFPLALMILAVLGSIVFGLATPSEAAAVGGFGGFVLAGIYRFIQ
                     YWREGRSTGSDTGKVIWSTVKELGVIVKESSFLTAKTSAMVCWLFVGSGIFSAAFALL
                     GGQEVIEKWVLSLNMTPLQFMILAQFIIFILGWPLEWTEIIIIFMPIFLPLLPHFGID
                     PLFFGLLVALNLQTAFLSPPVAMAAFYLKGVSPPHVTLNAIFAGMMPFMGIQVIALVL
                     LYMFPGIGLWLPQLVYGR"
BASE COUNT          679 a         1325 c         1309 g          671 t
ORIGIN      
        1 cgtggtgcag gatcattcgc cagccgttgc cggtgcgcag atacacgttg gtgacggcga
       61 ccggcgggcg cgagcgcggc tcgccctgta ccaggatgtt ctcgtgcacg ctgtggacgg
      121 cgagcatcat ccccgagagc accacttggt tggaaatgtg cactttgagc cgctggcctg
      181 acgcgaaaat ctgcgcccag ctctcgcgca cctgatcgta gcccgcgagc ctcgctcctc
      241 ccggatgcac gcacacgatc tcctcgtctt cggcccacac ttccatcatc gcatcgagct
      301 cacccgcctc gagcgcctcg tagaacgcgg cttctgcatc ctgcggcgtc ggaaaggtgc
      361 ttttcttcac gaggtgcggc tccggagccg cgtagcgtag ccgaaatggc tagtcgccgc
      421 cagcgcgcaa gacctgcacc gaacgcgttt ccgcgactca ccggggagaa tctttgaaac
      481 cgatcaccgc ttcgcaccag cgtgcgacgc gcaagagccg atcgtcgccg cccgcggggc
      541 cggcgagctg cattccgtag ggcatgccgc cgtcggacag gccggtaggc agcgtgaggg
      601 cggggaatcc aaggagcgtc cagagcgtgc agaagctcgg atctcccgtg acgtcgatcc
      661 gctccggagc tgcgccgggc gccggaagcg atgcgagcgc gtcgcagtct tcgaagaggt
      721 cgagcgcgag ctcggcgagc gctgcgcgcc tcacgagcgc gccgcgatag tcgtcgtgcg
      781 acatcgcaag cccctcgtcg atggcggcgt tcaacactgc gctcatgacg cgccgatagc
      841 tcgcctgcct ggtagcgtgc tcgcgggctc cctcgtagag catgatcgtg cggtgcaccc
      901 gatgcgcctc gtcgaagtcc tcgggcagcg ccaccgtttt caattccgtt ccggcgtcgg
      961 cgagacgctt caagctcgct tgcagatgcc gtgctgcgcc cggttccgcc gcgttccagg
     1021 gaaagcgcgg caagacggcg attttcggcg gacgcgagag cgcctcgatc tcgggagcaa
     1081 aggttccgga ctccgcgagg ctggcggcga agtaggcggc gtcggcgacc gtgcgcgcaa
     1141 acaaccctac atggtccagg gtttcggcaa agggaagcgc gccttgaatc ggcaggctgt
     1201 ccaggctcgg cttgaagccg actactccgc aaaacgccgc gggccggatg accgaaccgt
     1261 tggtctgcgt gcccacggcc accggaacat ggcgcgccgc gacggccgcg gccgacccgg
     1321 aggaggaacc gcccggcgtg tgtctggggt tccagggatt gcgcgtctcg gcgggatgca
     1381 tataggcgaa ttcggtggtg acggtcttgc cgagcacgta gccgccggcg gcgaggattc
     1441 ttccgacgag ctcggcgttt tttccaggct gattgccgac gaacgcgggc gagcccattt
     1501 ccgtgggcaa gtcgtcggtg tcgtagaggt ccttcacccc gaccggaaca ccgtgcagcg
     1561 cgccggtgtg tgcgccctgc gagcgttcgg cgtcgcgggc ggcggcgatg gtgagcgccc
     1621 gctcatcgtc gagtgcaatc cacgctttca gcctggggtc ggtcgcgcgg atgcgcgcga
     1681 ggcaggactc gcagtaggcc cgagcggcga gcgtaccgtc ggagagcgcc tgaacggttc
     1741 ccttcaagtc ctcgggcgag gccggcttcg cggctgggct cctgagcggc atgcggcgac
     1801 ggcgcgtcct agcggcgccg cttgggccag gcttccgggt tcagcaggtt gggcggtcgc
     1861 ctgcccgaca gcgcggcgcc taggttcctg ctcgcgagca tggccatcgc cgtgcgtgtc
     1921 gcacgcgtgg cgctgccgat atgcggaacc agggcgacgt tgtccacctc caggaacccg
     1981 ggattgaatt tcggctcgcc ttcgaacacg tcgaggcccg ccgctgcgat gcgccgttcc
     2041 ttgagcgcct ggacgagcgc cgcatcgtcg atgatcccgc cgcgagcagc attgacgatc
     2101 accgcagtcg gcttcatcag ggcaagctcg cgagcgccga caagatggtg gacttgcggc
     2161 gaatacggca tgttgaggct gacgaaatcg gattccttga ggagcttctc catgctcacg
     2221 tacttcgcgc ccagctgctt ttcgaccgcc ttcggcacgc gcctgcggtt atggtagaga
     2281 acacgcatgg agaacccctt cgcgcgccga gcgatcgcct ggccgatccg gcccatgccg
     2341 atgacgccga gcgtcgcgtg gtgcacgtcg acgccgagga actggtcgtt cttccaaccc
     2401 ttccagttcc cggcccgcag ccagcgctcg gcttcggtca cgcgccgcgc agcggccatg
     2461 agcagcgccc aagtcatgtc tgcggtcgta tcgtcgagca ctcccggcgt attcgtcgcg
     2521 aggatgcccc gcgcggtgca ggccgcgagg tcgatgttgt tgtagccgac tgcgatgttg
     2581 cacacggcct tcaagcgcgg cgccgcgtcc agcgtcgccg cgtccacgcg gtctgaaccc
     2641 gttggaagga cgccgtcctt gtcggcgagc cggcgccgga attcctcggc gctccagggc
     2701 gcatcgtcct tctggttgtc ctcgacttcg aagcgctcgc gcagcttcgc gatgacgtcg
     2761 tcgaaggtcg cgcgggtcac gagaatcttg ggtttcacgt cttctccgga aagccattcc
     2821 tgcggcaagc cggctgcgcc ggcagcaata cgaaatataa agtgattctc cggcaaaagg
     2881 aaaaggccgc tcgaagccgc ccttctctag cgagcgaacc cggattcagc ggccatagac
     2941 caactgcggc agccacaggc cgattcccgg gaacatgtac agcaggacca gcgcgatgac
     3001 ctggattccc atgaaaggca tcatcccggc gaagatcgcg ttgagggtca cgtgcggcgg
     3061 cgacacccct ttcaggtaga aagccgccat cgccaccggc ggcgacagga acgcggtctg
     3121 caggttgagc gccacgagca gcccgaagaa cagcgggtcg atgccgaagt gcggcagcag
     3181 cggcaggaag atcggcatga aaatgatgat gatctccgtc cactcgagcg gccagccgag
     3241 gatgaagatg atgaactgcg cgaggatcat gaactgcagc ggcgtcatgt tgagggacag
     3301 tacccacttc tcgatgacct cctgcccgcc gaggagcgcg aacgccgccg agaagatgcc
     3361 cgagccgaca aacagccagc acaccatcgc gctcgtcttg gcggtgagga aggacgactc
     3421 cttgacgatg acgcccagct ccttcacggt gctccagatc accttgccgg tatcgctgcc
     3481 ggtggatctg ccttcacgcc aatactggat gaagcggtag atccctgcca gcacgaatcc
     3541 gccgaatccg ccgaccgccg ccgcttccga cggcgtcgcc agcccgaaca cgatcgagcc
     3601 cagcaccgcc aggatcatca gcgccagcgg aaagaacgag gtgagcagca tcttgaacac
     3661 ttccagccgc tcccagctcc agagccagta gatgagcacg atgatggcga taccgaccgc
     3721 gaacgtgatc cagagccaga tgggtaccgg tagccgttcg gattctgcgg tcggcggacc
     3781 tttcgcggcc tccgcgcctg cttgaggagc agcgggcgcc ttcgaggact cggccgcagg
     3841 ctccttgacc ccggattctt ccggctcctg cgcgccgata aggcccgttg acacctcacg
     3901 ctcctgttcg ggcgccgcga tgaggcctcc cgcttcgatc agtccagctg tgctggcctc
     3961 gatctccggc gccgtcgccg cgcg
//
LOCUS       DASPZD010000119         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_307776, whole genome shotgun sequence.
ACCESSION   DASPZD010000119 DASPZD010000000
VERSION     DASPZD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307776"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VEM33_02280"
     CDS             <1..293
                     /locus_tag="VEM33_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYS50684.1"
                     /translation="DEKALFVGGRGKRISPAVVGARLRAWARRRGLVERVHPHMLRHS
                     FASHLLQSSQDLRAVQEMLGHASISTTQVYTHLDFQALAKVYDAAHPRAKKK"
     gene            290..1471
                     /locus_tag="VEM33_02285"
     CDS             290..1471
                     /locus_tag="VEM33_02285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HYS50685.1"
                     /translation="MIQLKKGREKSLRRRHPWIFSGAIERVTGKPGPGDTLEVRDASG
                     KPLAQAAFSPKSQIRARVWSFDANESIGADFFRNRLTRALAMREGLPAARHTNAMRLV
                     HGESDGMPGLVVDRYADVLVVQFLAAGVERWREPILDALVELTGCEAVFERSDAEVRT
                     LEGLEQRTGFARGNRAASRCPIIEYGLNFRVDVEQGQKTGFFLDQRENRQRVRALAAG
                     REVLDGFCYTGGFAIAALAGGAKRVTAIESSAPALEVAKENLAANPLDASRVDFVRTD
                     VFSELRTLRDRGARFDMVVLDPPKFAPTAAQARNAARAYKDINLLALKLLAPGGLLAS
                     FSCSGGVGAELFQSIVAGAALDAGGDAKIIERFCAASDHPVALEFPEGEYLKGLLVLK
                     T"
     gene            1532..>2033
                     /locus_tag="VEM33_02290"
     CDS             1532..>2033
                     /locus_tag="VEM33_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYS50686.1"
                     /translation="MQEQNMVPVTILTGFLGSGKTTLLNRILREDHGQRIAVIENEFG
                     EVGVDSDLIEKGDEQIVQMNNGCICCTVRGDLIRILGELKGKRDQGALKFDRVVIETT
                     GMADPGPVAQTFFTDDEIGNYYLLDSIITVVDAKHAPQQLDEFHEAQEQVGFADRLLL
                     SKTDLTN"
BASE COUNT          355 a          686 c          687 g          305 t
ORIGIN      
        1 cggatgaaaa agccctgttc gtcggcggcc ggggcaagcg gatctcgccg gcggtggtcg
       61 gcgcgcgcct gcgcgcctgg gcgcggcgcc gcggcctcgt cgagcgcgtg catccgcaca
      121 tgctgcggca ctcgttcgcc agccacctgc tgcagtcctc gcaggatctg cgcgcggtgc
      181 aggaaatgct cggccacgcc agcatctcga ccacgcaggt ctacacgcac ctcgacttcc
      241 aggcgctggc gaaggtctac gacgcggccc atcctcgcgc caagaagaaa tgatccagct
      301 gaagaaaggc cgcgagaaat ccctcaggcg gcgccatccg tggattttct cgggggcgat
      361 cgagcgcgtc accggcaagc cgggaccggg agacacgctc gaagtgcgcg atgcctccgg
      421 caagccgctc gcgcaggcgg cattcagccc gaagtcgcag atccgggcgc gcgtgtggag
      481 cttcgacgcg aatgaatcca tcggcgccga ttttttccgc aaccggttga cgcgcgcgct
      541 ggccatgcgc gagggacttc ccgccgccag gcacacgaac gcgatgcggc tggtgcacgg
      601 cgagtccgac ggcatgccgg gcctggtggt cgatcgctat gcggacgtgc tggtggtgca
      661 gttccttgcc gccggggtcg agcgctggcg cgagcccatc ctggacgccc tcgtcgagct
      721 gaccggctgc gaagcggtgt tcgagcgctc ggacgcggaa gtcagaacac tggagggcct
      781 ggagcagaga accggcttcg cccgcggcaa ccgcgccgcg tcgcgctgtc ccatcatcga
      841 gtacgggctc aacttccgcg tcgacgtcga gcagggacag aagaccggct tctttctcga
      901 ccagcgggag aaccggcagc gcgtgcgcgc gctcgccgcc ggccgcgaag tgctggacgg
      961 gttctgctac acgggcggct tcgccatcgc cgcgctggcc ggcggcgcaa agcgcgtgac
     1021 cgcgatcgag agctccgcgc cggcgctcga ggtggcgaag gagaacctgg cggcgaatcc
     1081 gctcgacgcg tcgcgcgtcg acttcgtgag aaccgatgtg ttttccgagc tgaggaccct
     1141 gcgagaccgc ggcgcgcggt tcgacatggt ggtgctcgat ccacccaagt tcgcgcccac
     1201 ggcggcgcag gcgcgcaacg ccgcgcgcgc ctacaaggac atcaacctgc tcgcgctcaa
     1261 gctgctcgcg cccggcgggc ttctggcgag cttctcctgc tctggcggcg tcggcgccga
     1321 gctgttccag tcgatcgtcg ccggcgccgc gctcgacgcc gggggggacg cgaaaatcat
     1381 cgagcgtttc tgcgccgcct ccgaccaccc ggtcgccctc gagtttcccg agggcgaata
     1441 cctgaaaggc ctattggttc tgaagaccta aatcagggac agaccacgtt tttgagacgg
     1501 ggtagaatcg cgcccttcgc caggagcacg catgcaagag cagaacatgg tcccggtgac
     1561 catcctcacg ggctttctcg gcagcggcaa gaccacgctg ctcaaccgca ttctcaggga
     1621 agaccacggc cagcgcatcg cggtgatcga gaacgagttc ggcgaggtcg gggtcgacag
     1681 cgacctcatc gagaagggcg acgagcagat cgtccagatg aacaacggct gcatctgctg
     1741 cacggtgcgc ggcgacctga tccgcatcct cggcgagctc aagggcaagc gcgaccaggg
     1801 cgcgctcaag ttcgaccgcg tggtgatcga gaccaccggc atggcggacc cggggccggt
     1861 ggcgcagacc ttcttcaccg acgacgagat cggcaactat tacctgctcg actcgatcat
     1921 caccgtcgtc gacgccaagc acgcgccgca gcagctcgac gaattccacg aggcacagga
     1981 gcaggtcggg ttcgcggacc ggctgctgct ctccaaaacc gacttgacca acg
//
LOCUS       DASPZD010000120         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1633094, whole genome shotgun sequence.
ACCESSION   DASPZD010000120 DASPZD010000000
VERSION     DASPZD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633094"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VEM33_02295"
     CDS             <1..323
                     /locus_tag="VEM33_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50687.1"
                     /translation="VAGATMMTGTVGPVAAVVIEVVHPALRATAASILALTQNLFGLA
                     AGPLLTGFLSDAYGLPVALSVVPSFSVFAAATFVAAARTYERDLLNVKLCVGPEAMST
                     ATRP"
     gene            complement(492..1640)
                     /locus_tag="VEM33_02300"
     CDS             complement(492..1640)
                     /locus_tag="VEM33_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50688.1"
                     /translation="MRTTVWCMLTALMLAANPATADFGTRGGSEPANIDEIADVLRQD
                     PYDMELLISFGTSKGGSAGHVALAIRDQAPGDDLVYTANFYADRAPKHAKGFYTDDLM
                     VRIPKKEYLFKTNSSLGDTTSFGLDFGEIYKRSVIGVRVYGVPAGEKEALAAFFRRIN
                     DDYHNRVRNTEYHNGEIEYDYLNLNCAKTIGAGFKYGGGYEKLEVKSAPVIRLRKVAA
                     AVRSNIPTEMAVKLLKEWNARGYGLDVVLYKKYEGSTYVDPHEEEKVAFKDLPNRFPS
                     VLSLDFRKEQGHYQDFDNLFAMYLLYHLGKYSVRVNDETKLLEVETSKNPMAYPEAAE
                     LATQSAKSDSENFRRRSAFRPSGTGVEEPTDNRHLYNFTDDGDKRSLR"
     gene            complement(1753..>3091)
                     /locus_tag="VEM33_02305"
     CDS             complement(1753..>3091)
                     /locus_tag="VEM33_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HYS50689.1"
                     /translation="TPVPAGLTAPAWHLFAVFVAAIASVLLGAFPLLTAAMLAVGTVV
                     LTGTITPAQAFSGFANSSVLLVVIAFIVAQAVVKSGLGRRISLFMVSRFGGSPIGLAY
                     SIVLTDAAIAPAFPSNTARGGVLFPIVLSLAKRSGSNPEDNEGRRFGGYLMFSTMASL
                     AISSALWMTASAVNPIGAQIVQKAGLQIGFGKWFLASCVPALVAIGMLPLLVSRLFPP
                     RVSATPEAPAAARAELAGMGSMSRDERITAVTFAVMVTGWVLADSLKLNVTSVAFMGL
                     GVLLVAGVLTLDDISKQGDTLVTFLWLAVLFALSGQLNELGFMGYVGERLASRLGGLS
                     WPATYVTLIALYVAIHYLFVSQSSQALALLGVFLDVGVRGGVPAPLMAYGLLFATCYF
                     SVITPQGGSQNIIFAASGYLTQRELYRLGLLTTLAFLAVFLVIGTPWVLFVTR"
BASE COUNT          594 a          978 c          941 g          578 t
ORIGIN      
        1 ttgtcgcggg cgcaacgatg atgaccggca cagtcggccc cgtggccgcc gtcgtcatcg
       61 aagtggtcca tcccgcgctc cgggcaacgg ccgcctccat tctcgcgctc acgcaaaacc
      121 tgttcggcct cgctgcgggg ccattgttga cgggttttct ttcggacgcc tacggattgc
      181 cggtcgcgct gtccgtcgtg ccatcgttta gcgtttttgc ggccgcgacg ttcgttgccg
      241 ccgcgcgcac ctacgagaga gacttgctga acgtcaagct ctgcgtaggt ccggaagcga
      301 tgtcgacggc cacgcgacca tgattcgaaa gtcctcggtc ttcgcgtcga acgtgtcgat
      361 ttgcagcgca ggtatgcggt tcacgcatgc acgatgatcc accggaccgc tcgaatcatt
      421 cgatcatctt cttgattacg gcccgacgct gctcccagcc tcacatcact taggtcgccg
      481 agcacgggca tctaacgcag actcctcttg tcgccgtcgt cggtgaagtt gtagaggtgt
      541 ctgttgtccg tgggttcctc gacccccgtc ccactcgggc gaaacgctga gcgccgacgg
      601 aagttctcgc tgtccgactt ggcgctctga gtggcgagtt cggcggcctc ggggtaggcc
      661 atggggttct tgctcgtctc cacctcgagc agcttcgtct cgtcgttcac ccgcacgctg
      721 tacttgccca agtggtagag caggtacatc gcaaagagat tgtcgaagtc ctggtagtgg
      781 ccctgctcct tccggaagtc gagggacaag acggagggaa agcggttggg cagatccttg
      841 aacgcaactt tctcctcttc atgcggatcc acgtaggtcg aaccctcgta cttcttatac
      901 agcaccacgt ccaacccgta ccctcgcgcg ttccactcct tcaaaagttt caccgccatc
      961 tcggtgggaa tgttcgacct gacagccgcg gccaccttcc gcagtctgat gaccggggcg
     1021 ctcttcactt cgagcttctc gtatcccccg ccgtacttga atcccgcacc gatggtcttt
     1081 gcgcaattca gattcaggta gtcgtactct atttcgccgt tgtggtactc ggtattcctg
     1141 acccggttgt gatagtcgtc gttgatacgt ctgaaaaacg ccgcgagtgc ctccttctcc
     1201 ccggcaggaa cgccgtagac gcgcacgcca ataaccgatc gcttgtagat ctcgccgaaa
     1261 tcgaggccaa atgaggtcgt gtcaccgagc gacgagttgg ttttgaacag gtactccttc
     1321 ttcggaatcc tgaccatgag atcgtccgtg tagaagccct tggcatgctt gggcgctcgg
     1381 tcggcgtaga aattcgccgt gtagaccagg tcgtcaccgg gcgcctggtc gcggatggcc
     1441 agggccacgt ggcccgccga gccgcccttg gaggtgccga agctaatcag caactccatg
     1501 tcgtacgggt cctgtcgcag gacatccgcg atttcgtcga tgttggcagg ttccgacccg
     1561 cccctggttc cgaagtcggc ggttgccgga ttcgcggcca acatgagcgc cgtcaacatg
     1621 caccaaaccg tcgttctcat ctcatcgctc tcacgagacc caaatttttg agaatcggtc
     1681 tttccgtctg caccgcatgc tatatcggcg agcatggcct ttttcgacgg atcgctcaag
     1741 tggccgccgt cgtcagcgcg taacgaacag cacccacggg gtcccgatca ccaggaacac
     1801 cgccaggaag gcgagggtgg tgaggagtcc cagcctgtag agctcgcgct gcgtcagata
     1861 gccgctcgca gcgaagatga tgttctggct tccaccctgc ggagtaatca ccgagaaata
     1921 gcaggtcgcg aagagcaggc cgtacgccat cagcggcgca ggcaccccac cgcgaacgcc
     1981 cacgtcgaga aaaacgccga gcagcgccag cgcctgcgac gactggctca cgaaaaggta
     2041 atggatggcg acgtaaagtg cgatcagagt gacatacgtt gccggccacg acaggccgcc
     2101 caggcgcgag gcgaggcgct cgccgacata gcccatgaaa ccgagctcgt tcagctgccc
     2161 gcttagtgcg aacagcaccg caagccagag gaaagtgacc agcgtgtcgc cctgcttgga
     2221 aatatcgtcg agcgtgagga cgcccgccac cagcagcacg ccgagaccca tgaacgcgac
     2281 gctggtcacg ttgagcttga gtgaatcggc gagcacccaa cctgtgacca tcacggcgaa
     2341 cgtgactgcc gtgatccgct cgtcccgcga catcgagccc atgccggcga gctcggcgcg
     2401 tgccgctgcg ggagcttcag gcgttgcgct gacacggggc ggaaagagtc tcgagacgag
     2461 caggggcagc atgccgatcg cgacgagcgc gggtacgcag gatgcgagga accattttcc
     2521 gaagccgatc tggagcccgg ccttctgcac gatctgcgca ccgatcggat tgacggcaga
     2581 ggcggtcatc cagagagcgg aggaaatcgc caggctcgcc atcgtggaga acatcagata
     2641 gccaccgaac cgccgtcctt cgttgtcctc ggggttggag cccgatctct ttgcgaggga
     2701 tagcacgatc gggaagagca caccgccccg cgccgtgttg ctgggaaagg ccggcgcgat
     2761 ggccgcgtcg gtcagcacaa tgctgtaggc gaggccgatg ggggagccgc cgaaccggct
     2821 taccatgaaa aggctgatcc gccgacccag tcccgacttc accacggcct gcgcgacgat
     2881 gaacgcgatc accacgagca gcacgctcga attggcgaaa cccgagaagg cctgcgccgg
     2941 cgtgatcgtc cccgtgagga cgacggtacc cacggccaac atcgctgccg tgaggagtgg
     3001 aaaggcgccc aggagcaccg acgcgatggc ggcgacgaag accgcgaaca gatgccacgc
     3061 cggcgcggtg aggccggctg gtaccggcgt g
//
LOCUS       DASPZD010000121         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1514829, whole genome shotgun sequence.
ACCESSION   DASPZD010000121 DASPZD010000000
VERSION     DASPZD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514829"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /gene="glyA"
                     /locus_tag="VEM33_02310"
     CDS             <1..973
                     /gene="glyA"
                     /locus_tag="VEM33_02310"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYS50690.1"
                     /translation="VYMAVLKPGDAILGMSLAHGGHLTHGAAVSASGKLYHALSYGLN
                     AEETIDYDGVERLAREHKPRMIVAGASAYSRVIDWRRLREIADAARAHLMADIAHYAG
                     LIAAGLYPSPIGIADFVTSTTHKTLRGPRGGLILAGAEHEKILNAAIFPCLQGGPLMH
                     VIAAKAVAFKEALAPEFRAYQKQVIANARILAKTLQARGVRIVSGGTDCHMLLVDLRA
                     KNVTGRDAEDALGQAHITVNKNAIPNDPQKPFVASGIRIGSPAMTTRGCRESEAAHLG
                     ELIADVLDAPRDDKIIKRVALEVSELCRRFPVYRAAEAGGGQRVAAA"
     gene            989..1471
                     /gene="nrdR"
                     /locus_tag="VEM33_02315"
     CDS             989..1471
                     /gene="nrdR"
                     /locus_tag="VEM33_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HYS50691.1"
                     /translation="MKCPYCKAEDTAVIDSRESEDGDSVRRRRQCETCGKRFTTYERV
                     ELSMPMVVKSSGNRVPYDREKLRTGFMRALHKRPVPTEYVDQAINRIEQRVLALGERE
                     VASRVIGEMVMRELKKMDDVAYIRFASVYEDFQRVDDFRDAILEVKKPGTKRARRIEK
                     "
     gene            1471..2553
                     /gene="ribD"
                     /locus_tag="VEM33_02320"
     CDS             1471..2553
                     /gene="ribD"
                     /locus_tag="VEM33_02320"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYS50692.1"
                     /translation="MFTAADHEFMRQALELAGRGLYTTTPNPRVGCVMVKDGTIVGAG
                     WHEKAGMPHAEVLALKAAGARARGATLYVNLEPCGHHGRTPPCADAIIDAGVGRVVAA
                     VQDPNPKVAGTGFSKLRAAGIAIEQGLREEEARELNIGFFARITRGRPWVRTKIAASL
                     DGRTALANGKSQWITGEAARADGHRWRARACAVLTGFGTVRDDDPQLNVRGVDTPRQP
                     LKIVVDSKFETSPSARLLQEGKTLVVGAVNDAKRVASLKAAGAEVVSIPNKSGKVELF
                     KLMEELARRELNEVHVEAGTKLNGSLLQAGVIDELLVYLAPSVVGDSGRGMFHLPEIT
                     ELSRVSALKIREVERVGADLRILARI"
BASE COUNT          487 a          856 c          920 g          425 t
ORIGIN      
        1 tgtctacatg gcggtgctga aaccgggcga tgcgatcctc ggcatgtcgc ttgcgcacgg
       61 cgggcacctc acgcacggcg ccgcggtgag cgcgagcggc aagctctacc acgcgctctc
      121 atacggcctg aacgccgagg agacgatcga ctacgacggc gtggagcgcc ttgcgcgcga
      181 gcacaagccg cgcatgatcg ttgccggcgc ctcggcctat tcccgcgtga ttgactggcg
      241 gcgactgcga gaaatcgccg acgcggctcg cgcgcacttg atggcggaca tcgcgcacta
      301 cgccgggctg atcgccgcag ggctctaccc gagcccaatc gggatcgcag acttcgtgac
      361 cagtacgacg cacaagacgc tgcgaggccc gcgcggaggg ctgatcctcg ccggagccga
      421 acacgagaaa atcctcaacg cggccatttt cccctgcctg cagggcgggc cgttgatgca
      481 tgtcattgct gccaaggccg tggcgttcaa ggaggcgctt gcacccgagt tccgcgccta
      541 ccagaagcag gtgattgcca atgccaggat tctggcgaag actttgcagg cgcgcggcgt
      601 gcgcatcgtt tcgggtggaa cggactgcca catgttgctg gtcgacctgc gcgcgaagaa
      661 cgtcaccggc cgcgacgccg aggacgcgct cggccaggcg cacatcacgg tcaacaagaa
      721 tgcgataccc aacgatcccc agaaaccctt cgtggcgagc ggcatccgca tcggctcgcc
      781 ggcgatgacc actcgcggct gtcgggagtc ggaagccgcg catctcggcg agctcatcgc
      841 ggacgttctg gatgctccgc gcgacgacaa gatcatcaag cgcgtggcgc tggaggtctc
      901 cgagctgtgc cgccgcttcc cggtataccg cgcggcggag gcgggtggcg ggcagcgcgt
      961 cgctgcggcc tagtgccgga gaagcgcggt gaaatgcccg tactgcaagg ccgaagacac
     1021 ggcagtcatc gactcgcgtg agtccgaaga cggcgacagc gtgcgccgcc ggcggcagtg
     1081 cgaaacctgc gggaagcgct tcacgaccta tgagcgcgtc gagctttcga tgcccatggt
     1141 ggtgaagtcg agcggcaatc gcgtccccta cgatcgcgag aagctccgca ccgggtttat
     1201 gcgcgcattg cataaacgac cagttcccac ggagtatgtc gaccaggcga tcaaccggat
     1261 cgagcagcgc gtcctcgctc tcggcgagcg cgaggtggcg agccgcgtga tcggcgagat
     1321 ggtgatgcgc gagctgaaga agatggacga cgtcgcctac atccgcttcg cctccgtgta
     1381 cgaggacttt cagcgcgtgg acgacttccg cgacgcgata ctcgaggtga agaagcccgg
     1441 caccaagcgc gcgcgccgca tcgagaagtg atgttcacgg ccgcggacca tgaattcatg
     1501 cggcaggcgc tcgaactcgc cgggcgcggc ctctatacga cgacacccaa tccgcgcgtc
     1561 ggttgcgtca tggtcaagga cgggacgatc gtcggcgcgg gctggcatga gaaggccggg
     1621 atgccgcacg cggaggtgct ggcactgaag gcagccggag cgcgcgcgcg gggcgcgacg
     1681 ctgtacgtga acctggaacc gtgcggccat cacggccgca ctccgccctg cgcggacgcg
     1741 atcatcgatg ctggcgtcgg gcgcgtggtg gctgcagtgc aggatccgaa tcccaaggtc
     1801 gcgggaaccg gcttctcgaa actgcgcgcc gcgggaatcg cgatcgagca agggctgagg
     1861 gaagaagagg cgcgcgagct caacatcggt tttttcgcgc gcatcactcg cggacgaccc
     1921 tgggtgcgca cgaaaatcgc ggcgagcctg gacggccgca ccgcgctcgc caacggaaaa
     1981 agccaatgga tcacgggcga ggcggcgagg gccgacgggc atcgctggcg cgcccgcgcc
     2041 tgcgccgtcc tcacgggttt cggcacggtc agggacgacg acccgcagct caacgtgcgc
     2101 ggcgtcgata cgcccaggca gccgctcaag atcgtcgtcg attcgaaatt cgagacgtct
     2161 ccttccgcgc gcctcctcca ggaggggaaa acgctcgtcg tcggcgccgt gaacgatgca
     2221 aagagggtcg cctcgctgaa agcggccggg gccgaggtcg tgagcatccc gaacaagagc
     2281 ggcaaggtcg agctgttcaa gctgatggag gagctcgcgc ggcgcgagct gaacgaggtt
     2341 cacgtcgagg cgggaacgaa actcaacggc tcgctccttc aggcgggagt gatcgatgag
     2401 ctcctcgtct acctcgcgcc gagcgtcgtc ggcgattcgg gacgagggat gttccatttg
     2461 cccgagatca ccgagctgtc gcgcgtaagc gcattgaaga ttcgcgaagt cgaacgcgtc
     2521 ggcgcggact tgcgcatcct ggcgcgcatc tagaagggcg aagagttatt tctcgccctt
     2581 gaacgcgcgc ttgaattcct gggcgccttc tttggccgca ccgccgacgg cctggcccgc
     2641 tttcttcgct tcgtggccga tctccttgcc ggcttttttt gcgccttg
//
LOCUS       DASPZD010000122         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1151432, whole genome shotgun sequence.
ACCESSION   DASPZD010000122 DASPZD010000000
VERSION     DASPZD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151432"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="ispH"
                     /locus_tag="VEM33_02325"
                     /gene_synonym="lytB"
     CDS             complement(<1..790)
                     /gene="ispH"
                     /locus_tag="VEM33_02325"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYS50693.1"
                     /translation="MEVLLANPRGFCAGVERAISIVERALAIHGAPIYVRHEVVHNRY
                     VVEDLKSKGAVFVEELTEVPPGSTVIFSAHGVSKAVRGEADARGLRVFDATCPLVTKV
                     HVEVAKMRAAGREIVMIGHRGHPEVEGTMGQAAEGMYLVETTADVARLDVRDPGKLSY
                     VTQTTLSVDDAAAIVEGLKARFPAITGPKRDDICYATQNRQDAVKVMAPQCDVVIVVG
                     SPNSSNSNRLREVARSRGIEAYMVDSAAQLKAEWVAGKRRVGVTA"
     gene            complement(802..1296)
                     /gene="lspA"
                     /locus_tag="VEM33_02330"
     CDS             complement(802..1296)
                     /gene="lspA"
                     /locus_tag="VEM33_02330"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYS50694.1"
                     /translation="MIPANLKRVAPWLALALLLALADQASKYAISASLRFGEVREIAP
                     FFNLVLAHNRGAAFSFLADAGGWQRALFIGIAAAATVAIVFLLARHSSERLFSAGLAL
                     VLGGALGNLWDRIALGHVVDFLDFHAFGWHFWAFNLADSAITVGAGLLILDGLCSAGA
                     AQKP"
     gene            complement(1293..4130)
                     /gene="ileS"
                     /locus_tag="VEM33_02335"
     CDS             complement(1293..4130)
                     /gene="ileS"
                     /locus_tag="VEM33_02335"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HYS50695.1"
                     /translation="MPENKTDYKKTLNLPDTPFPMRGDLARREPQWVKEWQEKKIYEK
                     IREASRGRPKFVLHDGPPYANGDIHIGQAVNKILKDVVVKSRNMAGFDAVYVPGWDCH
                     GMPIEVQIEKTYGKNIPVEKTLSLARAYAAEQIERQKKDFQRLGVLGDWEHAYTTMGY
                     RSEADEIRTLGKLLEKGYIYRGLKPVNWCFDCGSALAEAEVDYMDRTDFAVDVGFPLA
                     EDHRGKLARAFALDELPKGPVYAVIWTTTPWTIPANQALNVHPDYLYSLVETEKGSLV
                     LANDLKEACLSRFGLRRRAQSSAFDHNAPGKALELINFKHPFYERLSPVYLGEYVTLE
                     QGTGIVHSAPAYGVDDFVSCRRYGMKDEQILAPVLGDGKYASSLPFFGGMDIWTANSR
                     IVEKIREAGTLFHSEKHLHSYMHCWRHRTPIIYRATTQWFAGMDEVPGYKGAKPAETL
                     RSTALRGVDATRFYPSWGQARLHGMIANRPDWTLSRQRQWGVPMPFFIHRETGELHPR
                     TLELLEAVAKKVEQGGIEAWQRIEVKELLGADAERYEKVKDTLDVWFDSGSTHETVLK
                     GSHAKELKFPADLYLEGSDQHRGWFHSSLLVSSMLNGRAPYDALLTHGFVVDGEGRKM
                     SKSLGNVIAPRKVSETLGAEILRLWVAATDYSGELFISDEILKGVVESYRRIRNTLRF
                     LLANISDFDPVRHTIPVEKWLEIDRYAIVLATELQRNVLEDYKRYEFHPAVTRLLAFC
                     SEDLGAFYLDILKDRLYTCGADSSARRSAQNVLYHITQGLLRLMAPILSFTAEEAWKF
                     AGKGSESVFLHTWYELPRADDGDALLERWNLVRDARMHVQKKLETLRAGGKIGSSLQA
                     EIEVRASGAIHRALAALGNDLRFVMITSNASLALVSSGDEEVIAVPSPHPKCERCWHY
                     RADVDSDPAHPGICGRCVQNLYGSGEPRRFA"
     gene            complement(4123..>4619)
                     /locus_tag="VEM33_02340"
     CDS             complement(4123..>4619)
                     /locus_tag="VEM33_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HYS50696.1"
                     /translation="VETMPTVMHAGSRVSSSVVRAALAAGDLAAAETLLARTYSISGR
                     VIHGRKLGRELGFATANVQLKHNRPPLTGIYAAKVHGIGAAARPAVASLGVRPTITAS
                     GRAVLEVHVFDFSGDLYAAHVRVEFLHKIRDEEKYPDLETLKTQIARDCEAAKSFLMD
                     HRNA"
BASE COUNT          799 a         1494 c         1450 g          876 t
ORIGIN      
        1 cggccgtcac gccgacacgg cgcttccccg cgacccactc ggccttcagc tgcgcggcgc
       61 tgtccaccat gtaggcctcg atgccgcggc ttcgagcgac ctcgcgcagc cggttggaat
      121 tcgagctgtt gggcgagccg accacgatca cgacgtcgca ttgcggcgcc atgaccttga
      181 ccgcgtcctg ccggttttgc gtcgcatagc agatgtcgtc cctcttcgga cccgtgatcg
      241 cgggaaaacg cgctttcagc ccctccacga tcgccgcggc gtcatcgacc gagagcgtcg
      301 tctgcgtcac gtaggagagc tttcccggat cgcgcacgtc caggcgcgcc acgtcggcgg
      361 tggtttccac gaggtacatg ccttcggcgg cctggcccat ggtcccctcg acctccggat
      421 ggccgcggtg gccgatcatg actatttcgc ggccggccgc gcgcatcttc gccacctcga
      481 cgtgcacctt ggtgacgagc ggacaggtcg cgtcgaatac gcgcagtcca cgcgcgtcgg
      541 cctcgccgcg caccgccttc gagacgccat gggcgctaaa gatcaccgtg ctgcccggag
      601 ggacctcggt cagctcctcg acgaacacgg cgcctttgct cttcaggtcc tcgacgacgt
      661 acctgttgtg gacgacctcg tggcggacgt agatcggcgc gccgtggatc gcgagggcgc
      721 gctcgacgat gctgatcgcg cgctcgacac cggcgcaaaa accgcgtgga ttggcgagca
      781 gtacttccat tccggtctat tttagggctt ttgcgccgcg ccggccgaac acagcccgtc
      841 gaggatcagc agtcccgccc ctacggtgat cgccgaatcg gcgagattga aagcccagaa
      901 atgccagccg aaggcgtgaa agtcgagaaa atccaccacg tggccgagag cgatccgatc
      961 ccagagattg ccgagcgctc ctccgagcac caaggcaagt cccgccgaga aaaggcgctc
     1021 gctcgaatgc ctggcgagga gaaacacgat ggcgacggtc gcagcggcgg ctatcccgat
     1081 gaacagcgcc cgttgccagc cgcccgcgtc ggcgaggaag ctgaaagcgg caccgcggtt
     1141 gtgcgcgagc accaggttga agaacggcgc gatctcgcgc acctcgccga agcgcaggct
     1201 agcactgatc gcgtacttgc tcgcctggtc cgccagcgcg aggagcaggg cgagtgcgag
     1261 ccacggggcg actcgtttca ggttcgccgg gatcatgcga atcgccgggg ctcgccggac
     1321 ccgtagagat tctgcacgca gcgcccgcag attcctgggt gggccgggtc cgagtcaacg
     1381 tccgcacgat agtgccagca gcgctcgcat ttcgggtgcg ggctcggcac agcaatgacc
     1441 tcttcatctc cgctggaaac aagcgcaagc gaggcgtttg aggtgatcat cacgaagcgt
     1501 aggtcgttgc cgagcgctgc cagcgcccgg tggatcgctc cagatgcacg aacttcgatc
     1561 tccgcctgca gagacgaacc gatctttccg ccggctcgta aggtttcaag ttttttctgc
     1621 acgtgcatac gcgcgtcacg gacgagattc caccgttcga ggagcgcatc gccgtcgtcc
     1681 gctctcggca attcgtacca ggtgtgcagg aacacgctct cgctgccctt ccctgcgaat
     1741 ttccaggctt cctcggcggt gaagctcaga atcggcgcca tcaagcgcag gaggccctgc
     1801 gtgatgtggt agagcacgtt ctgcgccgag cggcgcgcgg aagaatccgc gccacaggta
     1861 tacagcctgt ccttgaggat atccagatag aacgcgccga gatcctcgga gcagaaggcc
     1921 agcagccgag tcacggccgg gtgaaattca tagcgcttgt agtcctcgag cacgtttctc
     1981 tgcagctccg tcgcgagcac gattgcgtag cggtcgattt cgagccactt ctccaccggg
     2041 atcgtgtgac gcacgggatc gaaatccgaa atgttcgcaa ggagaaacct cagcgtattg
     2101 cggatgcgcc ggtaggactc caccaccccc ttcaggatct cgtcggagat gaaaagctcg
     2161 cccgagtagt cggtcgccgc cacccagagg cgcaggatct ccgcgcccag cgtctcggag
     2221 actttccgcg gagcgattac gttgccgagg gacttggaca tcttgcggcc ttcgccgtcg
     2281 acgacgaagc cgtgcgtaag caacgcgtcg tagggcgccc ggccgttcag catgctggac
     2341 acgaggagtg aggaatggaa ccagccgcga tgctggtccg agccctcgag gtaaagatcc
     2401 gccggaaact tcagctcctt cgcgtgcgag cccttcagga ccgtttcgtg cgtcgaaccc
     2461 gaatcgaacc agacatcgag cgtgtccttc accttctcgt accgttcggc gtcggcgccg
     2521 agcagttctt tcacctcgat tcgctgccag gcctcgattc cgccctgctc cactttcttc
     2581 gcgaccgcct ccagaagctc cagcgtgcgc ggatgcagct cgcctgtttc ccggtggatg
     2641 aaaaacggca tcggcacgcc ccactggcgc tggcgcgaca gcgtccagtc gggacgattg
     2701 gcgatcatgc cgtgcaggcg cgcctggccc caggaaggat agaagcgggt cgcgtccacg
     2761 ccgcgcagcg cagtggagcg cagggtttcc gcgggtttcg cgcccttgta tccgggcacc
     2821 tcgtccatgc ccgcgaacca ctgcgtcgtg gcgcggtaga tgatcggggt cctgtggcgc
     2881 cagcagtgca tataactgtg caggtgtttt tccgagtgaa acagggtgcc cgcttcgcgg
     2941 atcttctcga cgatcctgga attcgccgtc cagatgtcca ttccgccgaa aaacggcagc
     3001 gaagaagcgt atttcccgtc gcccagcacc ggggcgagga tctgctcgtc cttcatgcca
     3061 tagcggcggc aggaaacgaa gtcatcgacg ccgtaggccg gggcgctgtg gacgatgccg
     3121 gtgccctgct ccagcgtcac gtactcgccg aggtagacgg gggaaagtct ctcgtagaaa
     3181 gggtgcttga agttgatcag ctcaagagcc tttccgggag cgttgtgatc gaacgcgctc
     3241 gattgagctc tccgcctcag tccgaagcgc gataaacagg cctccttgag atcatttgcg
     3301 agtaccagac tccccttctc ggtttcaaca agcgaataca ggtagtccgg gtgcacattc
     3361 aacgcctggt tggcgggaat cgtccagggt gtcgttgtcc aaatcaccgc atagaccggc
     3421 cccttcggta attcatcgag cgcgaacgcg cgcgccagtt ttccgcggtg atcctcggcg
     3481 agcggaaacc ccacgtcgac ggcaaaatcg gtgcggtcca tgtagtcgac ctcggcctcg
     3541 gccagcgcgg agccgcaatc gaagcaccag ttcaccggtt tcaggccgcg atagatgtag
     3601 cctttctcga gcaacttgcc gagcgtgcga atctcgtcgg cctcgctcct gtaccccatg
     3661 gtcgtgtagg cgtgctccca gtcgcccagg acgccgagcc gctggaagtc ctttttctgc
     3721 cgctcgatct gctcggccgc gtacgcgcgg gcgaggctca gggtcttttc cacgggaatg
     3781 ttcttgccgt aggtcttctc gatctgcacc tcgatcggca taccgtggca gtcccagccg
     3841 ggaacgtaga ctgcgtcgaa gcccgccatg ttgcgcgact tcacgacgac gtccttgagg
     3901 atcttgttga ccgcttggcc gatgtggatg tcgccgttgg catagggtgg gccgtcgtgg
     3961 agcacgaact tcggccttcc gcggctcgcc tcgcggatct tttcgtagat ttttttctcc
     4021 tgccattcct tgacccattg cggctcccgc ctcgcgagat cgccgcgcat gggaaagggc
     4081 gtgtccggaa gattcagcgt tttcttgtag tcggtcttgt tctcaggcat tgcggtgatc
     4141 catgaggaag ctcttggcgg cttcgcagtc gcgcgcgatc tgggtcttca gggtctcgag
     4201 atcgggatat ttctcctcgt cgcggatctt gtgcagaaat tcgacgcgca cgtgcgccgc
     4261 gtagagatcc cccgagaaat cgaacacgtg cacctccagc accgcgcgcc cgctcgccgt
     4321 gatggtgggc cgaacgccga gactcgcgac ggcagggcgc gcggcggccc cgatgccgtg
     4381 caccttcgcc gcgtagatgc cggtgagcgg agggcgattg tgcttcagtt gcacgttggc
     4441 ggtggcgaac cccagctcgc gtcctagctt tcgtccgtgg atcacgcgtc cgctgatgct
     4501 gtaggtccgc gcgagcagcg tctcggccgc cgcgaggtcg cccgcggcga gggcggcgcg
     4561 cacgaccgag ctcgataccc gcgacccggc gtgcatcacc gtcggcatgg tttcgactt
//
LOCUS       DASPZD010000123         8319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_790254, whole genome shotgun sequence.
ACCESSION   DASPZD010000123 DASPZD010000000
VERSION     DASPZD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790254"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VEM33_02345"
     CDS             complement(<1..957)
                     /locus_tag="VEM33_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYS50697.1"
                     /translation="MHASRRQFLKTASLMSMAGVAGPFALNLAAIGAASAQTAPYRAI
                     VCLFLYGGNDHTNTLIPYDQPSYDQYLAARDTIAIPRDQLTATATGAVASQGGREFAF
                     HPALTSFKTRWDEGKLAIVANVGPLIRPTTVAQYNARSVPLPPKLFSHNDQQSVWQAQ
                     APLGEGAKLGWGGRIGDLLASQNTNAQFTCISAGGNAVFLSGQNVIQYQVGSSGPVAI
                     SGISGTLYSSASASAAYRSLITGASANLFANELGVITNRSITSNQLLSASLPPASMFP
                     LPNPTSNLASQLNVVARIISARSQIGTNRQVFLVSLGGFDNHD"
     gene            complement(969..2597)
                     /locus_tag="VEM33_02350"
     CDS             complement(969..2597)
                     /locus_tag="VEM33_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HYS50698.1"
                     /translation="MPPSAYPLPTAADAARFLNQASFGATEAEVNKVRTYGYSAWLED
                     QFFMPGRSHEAYINAVSASLPAGQSLDDSHVMNTFWAEAATGQDQLRRRVAFALSQIL
                     VVSLQDSTVSEFKRGVASYLDMLGRNAFGNYRQLLNEVTLHPMMGLYLSHLRNRKEDP
                     ARNRVPDQNYAREVMQLFSIGLYELNPDGTLKLTSGQPVETYDTDDIVGLSRVFTGFS
                     WAGPDKSDTRFNGGGTQDPDREVLPMQSYPRFHSISEKRFLTAVIPAGATADPDGDLR
                     TALDTIATHPNVGPFISRQLIQRLVTSNPTPAYVERVAQAFDAGRFTSGLWSAGTGQR
                     GDLRATVAAILLDPEARTAAQLADPGFGKVREPILRVAQWMRAFNARSASGNFLLGVT
                     DDATASLGQSAMRSPTVFNFYRPGYVPPNTSIATAGLVAPELQIVNEVSVVGYSNFMR
                     SAAQNGLGSGSPRDIQPDYTAELALADNADLLIDRVSLLLTAGSMSAATRTIIRDAVN
                     SVAMPTTNQTTARQNRVRLAVFFTLSSPDYLAQK"
     gene            complement(2962..4020)
                     /locus_tag="VEM33_02355"
     CDS             complement(2962..4020)
                     /locus_tag="VEM33_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50699.1"
                     /translation="MGTKYKILIMGASYGSLLGTKLALAGHAVHLICLPAEADLINKE
                     GAIVRMPVKGRDGLVEINSKKLPGKMSAGGTAGVNPSEYHLVALAMQEPQYRSPGVRE
                     LLAAVAKAKVPCMSIMNIPPLPYLKRVPGIDADALRSCYTDASVWDAFDPKLVTLCSP
                     DPQAFRPPEEKVNVLQVRLPTNFKSARFDSETHTAILRRLESDIEAARFEGIELPVKL
                     KVHDSVFVPLAKWAMLLAGNYRCVLKDSVRPIKEAVHSDLEASRSVYNWVVKLCVSLG
                     ANEKDLVPFEKYASAALSLLTPSSAARALANGAPNIERTDRLVQTIAAQKGMRLAAVD
                     QTVALVDGWLERNRKKAA"
     gene            complement(4148..4915)
                     /locus_tag="VEM33_02360"
     CDS             complement(4148..4915)
                     /locus_tag="VEM33_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50700.1"
                     /translation="MRAARPTFGAFNQGGVPTIACFNKASTPLGVDFDALIAAMQAFV
                     DRCVAPVWGTPAKLVKSKGYVKGAWAMVFLDNADQPGALAYHDLTPDGMPQSKVFVKT
                     TLDNHDLVSVSASHELVEMLVDPAINIMTTGPDPKVMYAYESADPVEQLSFKVNGIPM
                     TDFVYPSYFEVFHKAGSVRFDQMKKVNKPFQILSGGYQIIFKNGTWSQIFASVSKKKR
                     FGREDRRGHRSEQRVRAARNKLRRADKKKIDRLARKN"
     gene            5040..5765
                     /locus_tag="VEM33_02365"
     CDS             5040..5765
                     /locus_tag="VEM33_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYS50701.1"
                     /translation="MQGKTVRVRSSEGGEFDCYRVAPRTGAKVPAIVIASAVHGVNAD
                     VRAIADEFASRGAIAAAPDLFWRSIPGPLARDDDRTKQRSQPRLEKIRTGEADLADTL
                     AGLRKEPLWNGRAAVMGLCYGGPYAIVGPKRLGYDAGISCHGSQMLDYIGELDGLELP
                     VCIIWGDADHQAPAEVLAAYRGAAARMKNLELHVFPGVQHGYMMPDNAKAFDRKTREF
                     SMERALAILEGLRAGKAGRRKAG"
     gene            5765..6007
                     /locus_tag="VEM33_02370"
     CDS             5765..6007
                     /locus_tag="VEM33_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50702.1"
                     /translation="MRASQRGLSAFGMVVVLAIAVAAGYYAYKSLSGSGDAPTCRSAS
                     TACIQKCRRTTTEAPAAQACQEGCQRDLTACERPGR"
     gene            complement(6025..6294)
                     /locus_tag="VEM33_02375"
     CDS             complement(6025..6294)
                     /locus_tag="VEM33_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HYS50703.1"
                     /translation="MAKHLVISGRVQGVGFRYSMSDEAERLGVTGWVRNRRDGTVEAV
                     IDGAPDAVEGILAWARRGPRGASVTGVEVSEIPGSFEGFELRPTE"
     gene            6365..6781
                     /locus_tag="VEM33_02380"
     CDS             6365..6781
                     /locus_tag="VEM33_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HYS50704.1"
                     /translation="MRFAAKMFAILALSFAGSAAWAQEKAVYHFDGGLEQATKGLRNI
                     NNHLEVDPKARLIAVAHADGVDFLLEGAKDRLGPFDARVQDLMGKGVKFQVCEITLRN
                     RKLKKDQFILGVEFVPSGVVQITHLQQKEGYAYLKP"
     gene            complement(6786..7226)
                     /locus_tag="VEM33_02385"
     CDS             complement(6786..7226)
                     /locus_tag="VEM33_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50705.1"
                     /translation="MELSLAFIGWLFTLTSAGALVLGAALITMLASAGDLQRRYLSYS
                     IWNDLVLAAIWVLGLAGGIGVIGLQPWGRYLLELFCWALIVLLPLSAASRLYALRQQP
                     DPGQPRANWLGAIAGITLILIPVLAICAATIVTLRSPEAMKAFS"
     gene            complement(7231..7743)
                     /locus_tag="VEM33_02390"
     CDS             complement(7231..7743)
                     /locus_tag="VEM33_02390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HYS50706.1"
                     /translation="MYQLLKQIAKTGIVTEPAPPPDAALRALEQRLGRAILEHFGRAL
                     AIRHVDAGSCNGCELEIHAMNSPYYNLEGLGIKFVASPRHADMLLVTGPVSRNMETAL
                     KRTWEATPDPKLVVAIGDCGCTGGIFGESYASCGRVSNVIPVDVSVPGCPPAPVAILQ
                     GILTAITPAR"
     gene            complement(7745..>8319)
                     /locus_tag="VEM33_02395"
     CDS             complement(7745..>8319)
                     /locus_tag="VEM33_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HYS50707.1"
                     /translation="RLARIEREVATLKSIYDEHAGAQDRFIMTGQVTPELAERMGLTG
                     LAARASGIARDLRVDYPIPPYQQLDPRAASHTRGDVSARVAVRFDEIFESMRLVQTLV
                     RQLPPGEFRAPVGAADAGARGLGWIEGWRGEVLVALDVSGTGRNHRLHPHDPSWQNWP
                     LLEHAVHGNIVPDFPLINKSFNLSYSGVDL"
BASE COUNT         1432 a         2640 c         2808 g         1439 t
ORIGIN      
        1 gtcgtggttg tcgaagccgc cgaggctgac caggaacacc tgccggttcg tcccgatttg
       61 gctccgcgcg gaaatgatgc gggcgacgac gttgagctgc gaggcgaggt tgctcgtggg
      121 gttgggcagg gggaacatcg atgccggcgg caaactggcg ctcagcagct ggttcgacgt
      181 aatcgatcga ttcgtgatca cgccgagctc gttcgcgaac aggtttgcgc tcgcgccggt
      241 gatgaggctg cggtaggcgg ccgatgcgct cgcggagctg tagagggtgc cggaaatacc
      301 cgagatcgcc accgggccgc tcgagcccac ctgatattgg atgacgttct ggcccgagag
      361 gaacacggcg ttgccgcccg ccgaaatgca ggtgaactgg gcgttcgtgt tctggctggc
      421 gagcaggtcg ccgattcggc cgccccagcc gagcttcgcg ccctccccga gcggcgcctg
      481 cgcctgccac acggactgct ggtcgttgtg cgagaacagc ttgggcggca gcggcacgga
      541 gcgggcgttg tattgcgcaa cggtcgtcgg ccttatgagc ggcccgacgt tggctacaat
      601 tgcgagcttg ccctcgtccc aacgagtctt gaacgaggtc agggccggat ggaaggcgaa
      661 ctcgcgccct ccctgggacg cgaccgcgcc ggtggcggtg gcggtgagct gatcgcgcgg
      721 gatcgcgatc gtgtcccggg ccgcaaggta ctggtcgtag gaaggctggt cgtacggaat
      781 gagcgtattg gtgtggtcgt ttccgccgta caggaaaagg cacacgatcg cccggtaggg
      841 ggcggtctgc gccgaggcag cgccgattgc ggcgaggttg agcgcgaaag gaccggccac
      901 cccggccatg gacatgagcg aagccgtctt gaggaattgg cgtcgggacg catgcatggg
      961 tcgctccgtt atttctgcgc cagatagtct ggcgacgaca gcgtgaaaaa caccgcgagc
     1021 cgcacccggt tctggcgcgc agtcgtttgg ttggtcgtgg gcatcgcgac cgagttgacc
     1081 gcgtcgcgga ttatcgtcct cgtcgccgcg ctcatggaac cggcggtgag gagcaggctg
     1141 actcggtcga tcagaagatc ggcgttgtcg gcgagcgcca gctcggcggt gtaatccggc
     1201 tggatgtcgc gcgggctgcc cgacccgagg ccgttctggg ccgccgagcg catgaagttc
     1261 gaatagccga ccaccgagac ctcgttgacg atctgcagct ccggggcgac gagccctgcc
     1321 gtcgcgatgg aggtgttcgg cggcacgtac ccgggccggt agaaattgaa caccgtcgga
     1381 gagcgcatcg ccgattggcc gagcgaggcc gtggcatcgt cggtgacccc gagcaggaaa
     1441 ttccccgagg cggagcgcgc gttgaaggcg cgcatccact gcgcgactcg gagaattggc
     1501 tcgcgaacct tgccgaagcc cgggtcggcg agctgcgccg ccgtccgcgc ttccgggtcg
     1561 agcaggatcg cagcgacggt ggcgcgcagg tcgcctcgct ggccggtgcc tgcgctccag
     1621 agccccgagg tgaagcgccc cgcgtcgaac gcctgggcga cgcgctcgac ataagccgga
     1681 gtggggttgc tggtcaccag gcgctggatg agctgccgag atatgaacgg gccgacattg
     1741 ggatgagtag cgatagtgtc gagcgcggtg cgcaggtctc cgtcgggatc ggcggtcgcg
     1801 cccgcgggga tcaccgccgt gagaaagcgc ttctccgaaa tcgagtgaaa gcgcgggtag
     1861 ctctgcatgg ggagcacttc gcggtccgga tcctgcgtgc cgccgccgtt gaagcgcgtg
     1921 tcggacttgt ccgggcccgc ccagctgaat cccgtgaaca cgcgcgacag gccgacgatg
     1981 tcgtcggtgt cgtaagtctc gaccggctgc ccgctcgtga gcttcagcgt gccgtccggg
     2041 ttcaattcgt agagcccgat cgagaagagc tgcatgacct cgcgcgcgta gttctggtcg
     2101 ggcacgcgat tgcgcgccgg gtcctccttg cggttgcgca ggtgcgacag atagaggccc
     2161 atcatcggat ggagcgtgac ctcattgagc agctggcggt agttgccgaa ggcgttccgg
     2221 ccgagcatgt cgaggtacga agcgacgccg cgcttgaact cggagacggt cgagtcctgg
     2281 agcgagacca ccaagatctg ggatagcgcg aacgcaacgc gccggcgcaa ctggtcctgg
     2341 ccggtcgcgg cctccgccca gaacgtattc atcacgtggc tgtcgtcgag gctctggccg
     2401 gcggggaggc tcgcgctcac ggcgttgatg tacgcctcgt gcgagcggcc tggcatgaag
     2461 aactggtcct cgagccaggc gctgtagccg taggttctga ctttgttgac ctcggcctct
     2521 gtcgcgccga aagaagcctg attgaggaag cgcgccgcgt ccgcggcggt cggcagggga
     2581 tatgcagacg gaggcacggt cgagagattg accccgctcc cctgcgaggc ggcttccggg
     2641 ctgcccgact tggagcatcc gcccgcaaca caggccagtg cgacggcgaa aaggacggcg
     2701 cagataggtt ggaagctgac ccctgtttgc ggtccgcgag agccagcgtc caggcgttgc
     2761 gtggagtccg gcgcggttgc gtgtcgtaac atcagtgcac cctcgatgta gtggcctccc
     2821 ctattgggtc acgctatctt aggcagaaac ttcgcagcat tatggccccg cgcaagcccg
     2881 ggcggggtag caattcgtac cggattccgg gactccctcg caattcgcgt gacgtagtgc
     2941 cgctttgtcc gcggcccgaa gtcaggccgc cttcttccga tttctctcca gccagccgtc
     3001 gaccagagcg actgtctgat cgaccgcagc caagcgcatg cctttctgcg cagcgatggt
     3061 ctgcaccagg cggtcggtgc gctcgatgtt gggcgcgccg ttcgccagag cccgcgcggc
     3121 ggaggacggg gtcaggagcg agagcgccgc gctggcgtac ttttcgaagg gcaccaggtc
     3181 cttctcgttc gcgcccaggc tcacgcacag cttcaccacc cagttgtata cagagcgcga
     3241 cgcttcgagg tcgctgtgca cggcctcctt gatggggcgc acgctgtcct tcagcacgca
     3301 gcggtagttg ccggcgagca gcatggccca cttcgcgagc ggcacgaaca ccgagtcgtg
     3361 caccttgagc ttgacgggca gctcgatccc ctcgaagcgg gccgcttcga tgtccgactc
     3421 gagccggcgg aggatggcgg tgtgagtctc cgaatcgaag cgcgccgact tgaagttggt
     3481 cgggagcctc acttgcagca cgttgacctt ttcctccggc ggacggaacg cctgcgggtc
     3541 cgggctgcac agcgtgacga gcttcggatc gaacgcgtcc catacgcttg cgtcggtgta
     3601 acagctccgc aaggcatcgg catcgatgcc cggaacgcgc ttgagatacg gcagcggcgg
     3661 tatgttcatg atcgacatgc agggcacctt ggccttggcc accgcggcga ggagctcgcg
     3721 cacgccgggc gagcgatact gcggctcctg catggcaagc gcgaccaagt ggtactcgga
     3781 gggattgacc ccggcggtac cgcccgcgga catcttgccg ggaagcttct tcgagttgat
     3841 ctcgacgagc ccatcacgac ccttgacggg catgcgcacg atcgcgcctt ccttgttgat
     3901 cagatcggct tcggcgggaa ggcagatcag atgcactgca tgccccgcaa gagcgagctt
     3961 ggtcccgaga agcgaaccgt acgaggcgcc catgatcaga attttgtatt tggtgcccat
     4021 caaccaactc ctcctttcac cgtcttgcga agtctgggat ggtagtcagt tcgctgctac
     4081 agagcaatca tgacgcgagc attcggattg cggcgctgcg tcatcggaga acctgtccgc
     4141 gccagcgcta attcttgcgc gccaagcggt cgattttttt cttgtcggcg cggcgcagct
     4201 tgttccttgc cgctcgcacg cgctgttcgc tccgatggcc gcgccggtcc tcgcggccaa
     4261 agcgcttttt cttagaaacc gaggcgaaga tctggctcca cgttccgttc ttgaagatga
     4321 tctgatagcc gccggaaagg atctgaaacg gcttgttgac cttcttcatc tgatcgaagc
     4381 gcaccgaacc ggccttgtgg aaaacctcga aataggacgg atagacgaag tccgtcatcg
     4441 gaatgccgtt gaccttgaag ctgagctgct cgaccgggtc ggcgctttcg taggcataca
     4501 tgaccttggg atcggggccc gtcgtcatga tgttgatcgc cgggtcgacc agcatctcga
     4561 cgagttcgtg tgacgccgac acgctaacga ggtcgtggtt atccagggtc gtcttgacaa
     4621 aaaccttgga ctgcggcatc ccgtcgggcg tgagatcgtg atacgcgagg gcgcccggtt
     4681 gatcggcatt gtcgaggaaa accatcgccc aagcgccttt cacgtatccc ttggacttga
     4741 ccagcttcgc cggcgtgccc cacaccggcg ccacgcatcg atccacgaac gcctgcatcg
     4801 cggcgatcaa agcgtcgaag tcgaccccca gaggcgtcga cgccttgttg aagcacgcga
     4861 tggtgggaac tcctccctgg ttgaatgcac cgaacgtcgg tcttgcggct ctcatgaagt
     4921 cctccttggg aaatgccggg agaatccgca tcctgccaca tctgtcgctg cgagttggac
     4981 aaggcgctcg cgctggagta cggtgagcgc cgtttcggcg cgagacgaga ggaggcgaca
     5041 tgcagggcaa gactgtcagg gttcgttcga gcgagggcgg cgagttcgat tgctatcggg
     5101 tcgcgccccg caccggcgcg aaggtcccgg cgatcgtgat cgcttccgcg gtgcacggcg
     5161 tgaacgcgga cgtccgcgcg atcgccgacg agttcgcctc gcgcggcgcc atcgcggcgg
     5221 cgcccgatct gttctggcgc tcgatcccgg ggccgctcgc gcgcgacgac gatcgtacga
     5281 agcagcgctc gcagccacgc cttgaaaaaa tcaggacggg agaagccgac ctcgccgata
     5341 cgctcgccgg tcttcgcaag gagccgctgt ggaacgggcg cgccgcagtg atgggcctgt
     5401 gctacggcgg cccttatgcc atcgtcggac cgaagcgcct gggctacgac gcgggcatct
     5461 cctgccacgg ctcccagatg ctcgactaca tcggggagct cgacggcctc gagctccccg
     5521 tctgcatcat ctggggcgat gcggaccacc aggcaccggc cgaggtgctg gccgcctatc
     5581 gcggggccgc cgcgcgcatg aagaacctgg agctccacgt tttcccgggc gtgcagcacg
     5641 gctacatgat gccggacaac gccaaggcgt tcgatcggaa gacccgcgaa ttctcgatgg
     5701 agcgcgcgct cgcgatactc gagggacttc gcgccgggaa agcgggccgg cgcaaggcgg
     5761 gctgatgcga gcgtcgcagc gcggcctgag cgccttcggc atggtcgtcg tcctggcgat
     5821 cgccgtcgca gcgggttact acgcgtacaa gtctctctcg ggaagcggcg acgcgcccac
     5881 ctgcaggagc gcctccaccg cctgcatcca gaaatgccgg cgcacgacga ccgaagcccc
     5941 tgccgctcag gcctgccagg agggctgcca gcgagacctc acggcgtgcg aacgaccggg
     6001 caggtgagtg acagcggccc gccgctattc ggtcggccgc agctcgaatc cctcgaagct
     6061 tccgggaatt tccgatactt cgaccccggt cacgctcgcg ccgcgcggcc cgcggcgcgc
     6121 ccaagcgaga atcccttcca ctgcatccgg cgctccgtcg atgacggcct cgaccgtccc
     6181 gtcgcggcgg ttcctcaccc agccggtgac accgagccgc tcagcttcat cgctcatcga
     6241 gtagcggaat cccacgccct ggacgcggcc cgagatcact agatgtttcg ccatccgctg
     6301 attttctaat cctggcgggt tcgcgtaaag tgagcgccgt tgacgattgt gaaaggagca
     6361 tgccgtgaga ttcgcagcca aaatgttcgc catcctcgcg ctgtcctttg cggggtccgc
     6421 cgcctgggcg caggagaaag ccgtttacca cttcgacggc ggcttggaac aggcgaccaa
     6481 gggcctgcgc aacatcaaca accatctcga agtcgacccc aaggcgaggc tcatcgcggt
     6541 ggcccacgcc gacggcgtcg atttcctgtt ggagggcgcc aaggaccgcc tcggcccctt
     6601 cgacgcgcgc gtacaggatc tgatgggcaa gggcgtgaag ttccaggtgt gcgagatcac
     6661 gttgcgcaac cgcaagctga agaaggacca gttcatcctc ggcgtcgagt tcgtgccctc
     6721 gggcgtggtg cagatcacgc atcttcagca aaaggaaggc tacgcctacc tgaaaccctg
     6781 agccgtcagg aaaacgcctt catcgcctcg ggactgcgca gcgtgacgat ggtcgcggcg
     6841 cagatcgcaa gcacgggaat tagaatcaat gtgatgcccg cgatggcccc cagccaattg
     6901 gcgcggggct gccccgggtc gggctgctgc ctgagagcgt aaagccggga agccgcggag
     6961 agcggcagca gcacgatcag cgcccagcag aagagctcca gcagatatct tccccacggt
     7021 tgcaggccga tcacgccgat tcccccggcg agccccagca cccagatcgc cgcgagcacc
     7081 agatcgttcc agatgctgta gctcagatag cgccgctgca gatctcccgc gctcgcgagc
     7141 atcgtgatca gcgcggcccc cagaacgagc gcgccggccg aagtgagagt gaacaaccag
     7201 ccgatgaacg cgagggagag ttccatcgct ttagcgggcc ggggttatcg cggtaaggat
     7261 gccttggagg atcgcgacgg gggcaggcgg gcacccggga acgctgacat ccacggggat
     7321 gacgttcgag acccggccgc agctcgcgta gctctcgccg aagatccccc ccgtgcaacc
     7381 gcagtcgccg atcgcgacca ccagcttcgg gtcgggagtg gcctcccagg tgcgcttgag
     7441 cgcggtttcc atattcctcg acacgggacc ggttacgagc agcatatcgg cgtggcgcgg
     7501 gctcgcgacg aacttgatgc cgagaccctc gagattgtag tagggactgt tcatcgcgtg
     7561 gatctcgagc tcgcagccgt tgcacgagcc cgcatccacg tgccggatcg ccagcgcgcg
     7621 gccgaagtgc tcgagtatgg cccgcccgag ccgctgctca agcgctcgca gcgccgcgtc
     7681 cggaggcgga gcgggctcgg tgacgattcc ggtcttcgcg atctgtttca gcagctggta
     7741 catttcacag atccactccg ctatagctca ggttgaacga cttgttgatg agcgggaagt
     7801 ccgggacgat gttgccgtgc acggcgtgct cgaggagcgg ccagttctgc cacgagggat
     7861 cgtgcggatg caggcggtgg ttgcgacctg tgcccgagac atcgagggcg accagcacct
     7921 ctccgcgcca gccttcgatc cagcccaggc cgcgcgcacc cgcatcggcc gcaccgaccg
     7981 gcgcgcggaa ctcccctggg ggcagctgcc gcacgagcgt ctgcacgagg cgcatcgatt
     8041 cgaagatctc gtcgaagcgc accgcgacgc gcgccgagac gtcgccgcgc gtgtgcgaag
     8101 cggcgcgcgg atcgagctgc tgatagggcg ggatcgggta atcgacgcgc agatcccgcg
     8161 cgatgccgct cgccctggcc gcaagcccgg tgagacccat ccgctcggcg agctcgggcg
     8221 tcacctgtcc ggtcatgatg aagcggtcct gcgcgcccgc gtgctcgtcg tagatcgatt
     8281 tcagggtcgc gacctcgcgc tcgatccgcg ccagacgct
//
LOCUS       DASPZD010000124         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1073653, whole genome shotgun sequence.
ACCESSION   DASPZD010000124 DASPZD010000000
VERSION     DASPZD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073653"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEM33_02400"
     CDS             <1..203
                     /locus_tag="VEM33_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HYS50708.1"
                     /translation="KCSLRHNAAAVIFAHNHPSGVAEPSHADEILTRSLKSALALVDI
                     QVLDHFIVAGSRTMSFAERGLL"
     gene            295..528
                     /gene="rpmB"
                     /locus_tag="VEM33_02405"
     CDS             295..528
                     /gene="rpmB"
                     /locus_tag="VEM33_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYS50709.1"
                     /translation="MARVCQVTGKKPQVGHHVSHANNKTIRRFYPNLQYRRFWVESEK
                     RWVRLRTSNAGVRIIDKKGIDAVLKELRERGEI"
     gene            546..701
                     /gene="rpmG"
                     /locus_tag="VEM33_02410"
     CDS             546..701
                     /gene="rpmG"
                     /locus_tag="VEM33_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HYS50710.1"
                     /translation="MREKIKLESTAGTGHFYTTTKNKRTTPDKIEMKKYDPRARKHVV
                     YKETKIK"
     gene            complement(780..1055)
                     /locus_tag="VEM33_02415"
     CDS             complement(780..1055)
                     /locus_tag="VEM33_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HYS50711.1"
                     /translation="MKSLIAVAASMLIASPVLAQTKPCEELKSEIEAKIKANGVPVFT
                     LDIVPNEQVKEGEGKVVGTCDGSTQKIVYKRGVAEAPRDMPAKSQPK"
     gene            complement(1206..2393)
                     /locus_tag="VEM33_02420"
     CDS             complement(1206..2393)
                     /locus_tag="VEM33_02420"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HYS50712.1"
                     /translation="MESSVLFSGVFDLPWWGLILAAIGLTHITIVSVTVFLHRCQAHR
                     ALDLHPLASHFFRLWLWMTTGMVTKEWAAIHRKHHAKCETAEDPHSPQQVGINRVLWG
                     GVFLYVKESYNRETMTRYGHGTPDDWLERNIYSRFSILGITLMGAVNVMLFGIVPGAL
                     ILVTQIAWIPFWAAGVINGIGHFWGYRNWPTEDASTNIVPWGIIIGGEELHNNHHAYA
                     TSAKLSNKWYEIDLGWMYICLLEALGLAEVKKVAPTPRFTDAKPAVDSETLQAVITHR
                     YDVLAKYAKSLKRTYADELGKLRQLAPHDAHVLKSLKRWLHRDEKSLCETERANLKQG
                     LAKSRALHTVYSMRAELVSLWERSSASREQLVRQLQDWCHRAEASGIRPLAEFSHRLR
                     RYA"
     gene            complement(2600..>3136)
                     /locus_tag="VEM33_02425"
     CDS             complement(2600..>3136)
                     /locus_tag="VEM33_02425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HYS50713.1"
                     /translation="LGALMRSTGRLYAFDISPKRLAGLKPRLARSGLSNVHPQRIEGV
                     NDARVKRLAGKIDRVLVDAPCSGLGTLRRNPDMKWRQTPEDVAELVEKQRSILAAAGR
                     LVKPGGRLVYATCSILEEENRGVVEDFLRNAPFTLRPAGDVLEKSGIRLHLDDYLELW
                     PHRHGTDAFFAAVLERAE"
BASE COUNT          607 a          990 c          947 g          592 t
ORIGIN      
        1 tgaagtgctc cctgcggcat aacgcggcgg cagtcatttt cgcccataac cacccctctg
       61 gggtcgccga gccgagccat gccgacgaga tcctcacacg ctcgctgaag agcgcgctcg
      121 ccctggtcga tattcaggtg ctcgaccatt tcatcgtcgc agggagccgc acgatgtcct
      181 ttgccgagcg cgggctgctg tgaagatcca cgacggatcc ttcgccgcga cgccgagctt
      241 gaacgcccgc ccgttttcga cctataatgc gccccttttc ccgcggagca tcgaatggcg
      301 cgcgtctgcc aagtaacggg caagaagccc caggtgggac accacgtttc ccacgccaac
      361 aacaagacca tacgccggtt ctacccgaac ctgcagtacc gccgtttctg ggtggaaagc
      421 gaaaagcgct gggtgaggct gcgcacttcc aacgccggcg tgcgcatcat cgacaagaag
      481 ggcatcgacg cggtgctcaa agaactgcgc gagcgcggcg agatctaggg ccgggagccg
      541 cgacgatgcg cgagaaaatc aagctcgaat ccacggccgg caccggccac ttctacacca
      601 ccaccaagaa caagcgcacc acgcccgaca agatcgagat gaagaagtac gaccccaggg
      661 cgcgcaaaca cgtcgtctac aaggaaacaa aaatcaaata aaagcccgcg aaagcgggct
      721 tttcatttgg cttcaggctc aggccggcgg agcctgagcc ggcgtccggc caggcgcccc
      781 tacttcggct gcgatttggc tggcatgtcg cgcggagcct cggcaacccc gcgcttgtac
      841 acgatcttct gggtgctgcc gtcgcaggtt ccgacgacct tgccttcacc ttcctttacc
      901 tgctcgttcg gaacgatgtc cagcgtaaag accggtacgc cgtttgcctt gatcttcgcc
      961 tcgatctcgg acttgagttc ctcgcaaggc ttcgtctgcg caaggaccgg cgaagcaatc
     1021 aacatcgacg cagctaccgc gataaggctt ttcatcccac gtctcctttt gctgtaaata
     1081 gcccccccga aaccgaccga cgccgcatat gctacccgga agtgcgttgg cgcgctgtga
     1141 aacaaaaaac ccgcctttcg ggcgggtttt tcgtggcctc ccgctttccg cgggattgaa
     1201 gcgccctagg cgtagcggcg cagccggtgc gagaattcgg cgagcgggcg gatgccgctc
     1261 gcttcggcgc ggtggcacca gtcctggagc tgccgaacga gctgctcgcg cgaggcgctc
     1321 gagcgctccc acagcgacac gagctcggcg cgcatcgagt acacggtgtg cagcgcgcgc
     1381 gatttcgcca gcccctgctt caggttcgca cgctcggttt cgcacaggga tttttcgtcg
     1441 cggtggagcc agcgcttcag actcttcagc acgtgcgcgt cgtgcggcgc gagctggcgc
     1501 agcttgccga gctcgtctgc gtaggtgcgc ttgagcgact tggcgtattt cgcgagaacg
     1561 tcgtaacggt gcgtgatcac cgcctgcagc gtctccgagt ccactgctgg cttcgcatcg
     1621 gtgaaacgcg gcgtcggcgc cactttcttc acctcggcga ggccaagcgc ctcgagcagg
     1681 cagatgtaca tccaacccaa gtcgatctcg taccacttgt tggaaagctt cgccgaggtg
     1741 gcatacgcgt ggtggttgtt gtgcagctcc tcgccgccga tgatgatgcc ccagggaacg
     1801 atgttcgtgc tcgcgtcctc ggtcggccag ttacggtatc cccagaaatg tccgatgccg
     1861 ttgatgacgc cagccgccca gaacgggatc caggcgatct gcgtaacgag gatcagagca
     1921 cccggaacga tgccgaacaa catgacgttg acagcgccca tcagggtgat gcccagtatc
     1981 gagaaacggg agtagatgtt gcgctcgagc cagtcgtcgg gtgtgccgtg gccgtaacgg
     2041 gtcatcgtct cgcggttgta ggattctttg acgtacagaa acaccccgcc ccagagtacg
     2101 cggttgatgc cgacctgctg agggctgtgc gggtcttcgg cggtttcgca tttggcgtgg
     2161 tgcttgcggt gaatcgcggc ccactccttg gtcaccatgc cggtcgtcat ccacagccac
     2221 agacgaaaga agtggctcgc gagcggatgc agatcgagcg cgcggtgcgc ctggcagcga
     2281 tgcaggaaca ccgtcacgct gacgatggtg atatgggtaa gaccgatcgc ggcgaggatc
     2341 aaaccccacc acggcaggtc gaagacgcct gaaaacagga cgctggattc catcaagctc
     2401 cccctctgag attcagcgga ttctacgtga atccccataa agggcaaggg cattatcccc
     2461 tatgcttcac tcagggattt cctgatccta ttcatgtcct tgcaagatcg caaattcacg
     2521 tgcatttagt ttcaatacgt gagattttca cgcaagatat gtagaaaata atcctgcgcg
     2581 gaaggccttg gttcgtggct cattcggcac gctcgagcac cgcggcaaag aacgcgtcgg
     2641 tgccgtgccg gtgcggccaa agctcgagat agtcgtctaa atggagccgg atgccgcttt
     2701 tttcaagcac gtcgcccgca ggccgcagcg tgaagggggc gttgcgcaga aaatcctcga
     2761 cgacgccgcg gttttcttcc tccaggatgc tgcaggtggc gtaaacgagc cgaccgcccg
     2821 gcttcacgag ccgcccggcc gcggcgagaa tcgatctctg cttttccacg agctccgcaa
     2881 catcctcggg cgtctggcgc cacttcatgt cgggattgcg ccgcagcgtt ccgagtccgc
     2941 tgcagggtgc atccacgagc acgcggtcga tcttgccggc gaggcgcttc acgcgcgcgt
     3001 cgttcacgcc ctcgatccgc tgcgggtgca cgttggacaa tcccgagcgc gcaagccggg
     3061 gcttcagccc ggcaagccgc ttcggggaga tgtcgaacgc gtagaggcgt cccgtcgagc
     3121 gcataagggc cccgag
//
LOCUS       DASPZD010000125         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1632127, whole genome shotgun sequence.
ACCESSION   DASPZD010000125 DASPZD010000000
VERSION     DASPZD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632127"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VEM33_02430"
     CDS             complement(<1..598)
                     /locus_tag="VEM33_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYS50714.1"
                     /translation="MSASGLSLLKPHTRDLGGFSVRRILPGMPHRMVGPFIFFDHMGP
                     ADFAPGSGIDVRPHPQIGLATVTYLFEGEILHRDSLGTVQAIAPGDVNWMTAGRGIVH
                     SERTAPELRRTGTRLQGLQTSVALPKRNELADPAFSHHPKATLPRVSAPGVELRVVAG
                     RAFGKRSPAPVFSQTLFVGVEIDAGRAFELEAGHEERAV"
     gene            complement(658..>2083)
                     /locus_tag="VEM33_02435"
     CDS             complement(658..>2083)
                     /locus_tag="VEM33_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYS50715.1"
                     /translation="GILSAHARTERYFGQDDASFLQSVANILAVAIERRSAEERLERL
                     AQFDSLTGLPNRHLFHDRLLKTVAHARRSGAPMAVLFIDLDRFKLVNDTQGHSAGDKL
                     LKEAADRLLQCVRSGDTVGRFGGDEFGAIVSELAKPGDAGVVAQKVLDALAQPFKLDA
                     YDTYVSASIGITLFPADGDNPESLVMNADTAMYRAKEQGRNTYQYFTREMNERALARV
                     RMEAALRRAIDHKEFLLHYQPKVNLQSRLICGFEALLRWQHPDKGLVLPGEFVSVLED
                     TGLIVSVGEWVLREVCEQIQVWQRAGLAVTPVTVNVSARQFQQKDFESIVRRILREAG
                     LDPSLVQFELTESLLMSDPEGAARTLRGLKDSGVKISVDDFGTGYSSLAYLKRFPIDA
                     LKIDHSFIRDITTDPEDAMITLAIIGLAHSLRLRVVAEGVETQEQLELLAANGCDEIQ
                     GYYFSIPTTAEECERMLKENRRLA"
BASE COUNT          339 a          683 c          692 g          369 t
ORIGIN      
        1 atacggcgcg ctcctcgtgc ccggcttcga gctcgaaggc gcggcccgcg tcgatctcta
       61 cgccgacgaa gagggtctgg ctgaacacgg gtgcgggcga gcgcttgccg aaagctctgc
      121 cagcgacgac acgcagttcc acgcccgggg cggaaacccg cggcagcgtc gccttggggt
      181 gatggctgaa cgcgggatcg gcgagctcgt tccttttcgg tagcgcgacc gaggtctgca
      241 ggccctgcag gcgcgtgccc gtgcggcgca gctccggcgc ggtgcgctcc gaatggacaa
      301 tgccgcggcc ggcggtcatc cagttgacat cgcccggtgc gatcgcctgc accgtgccga
      361 ggctgtcgcg gtgcaggatc tcgccttcga acagataggt gaccgtcgcc aagcctatct
      421 gcggatgcgg gcgcacgtcg atgccgctgc ccggcgcgaa atccgcgggg cccatgtgat
      481 cgaaaaagat gaacggaccg accatgcggt gcggcatccc ggggaggatc ctgcgcaccg
      541 agaacccgcc gaggtcgcgg gtgtgcggtt tgagcaggga aaggccgctt gcgctcatgg
      601 ggggccgcgg acaaagcggc gatgatacgc ttctcggaac cggggcgcga gttcgcctca
      661 ggcgaggcgg cggttttcct tcagcatcct ctcgcactcc tcggctgtcg tcggtatcga
      721 gaagtaatag ccctggatct cgtcgcagcc gttcgcggcg agcagctcga gctgctcctg
      781 cgtttccacg ccttcggcca cgaccctgag cctgaggctg tgcgcgaggc cgatgatggc
      841 gagcgtgatc atcgcgtcct ccgggtcggt ggtgatgtcg cgaatgaagg agtggtcgat
      901 cttgagcgcg tcgatcggga aacgcttcag gtacgcgagg ctcgaatagc ccgtgccgaa
      961 atcgtccacc gagatcttca cgcccgagtc tttcaacccg cgcagcgtgc gcgcagcgcc
     1021 ctccggatcg ctcatcagga gggactcggt gagctcgaac tggacgagcg aagggtcgag
     1081 ccccgcttcc cgcaagattc gcctcacgat ggactcgaaa tccttctgct ggaactggcg
     1141 cgcggaaacg ttcaccgtca cgggcgtgac agcgagtccc gcccgctgcc atacctgaat
     1201 ctgctcgcac acttcgcgca acacccattc gcccacgctc acgatcagac ccgtgtcctc
     1261 cagaaccgaa acgaattccc cgggcaggac caggcccttg tcgggatgtt gccagcgcag
     1321 cagcgcctcg aaaccgcaga tcaggcggct ctgcaggttc accttgggct ggtaatgcag
     1381 gaggaactcc ttgtgatcga tggcccggcg cagcgccgcc tccatccgca cgcgggcgag
     1441 cgcccgctcg ttcatctcgc gggtgaagta ctggtaggta ttgcggccct gttccttggc
     1501 gcgatacatc gccgtgtcgg cgttcatcac cagggattcg gggttgtcgc cgtcggccgg
     1561 gaacagcgtg atgccgatgc tcgcgctgac gtaggtgtcg tacgcatcga gcttgaacgg
     1621 ctgggcgaga gcgtccagga ctttttgtgc aacgacgccc gcgtcccccg gtttcgcaag
     1681 ctccgacacg atggcgccga actcgtcccc gccgaaacgg cccaccgtgt cgccgctgcg
     1741 cacgcactgc aaaagccggt ccgcggcttc cttgagcagc ttgtccccgg cactgtgccc
     1801 ctgagtgtcg ttcaccagct tgaagcggtc gaggtcgatg aacagcactg ccatgggcgc
     1861 cccgctgcgc cgtgcgtgcg ccacggtttt cagcaggcgg tcgtggaaca ggtgccggtt
     1921 gggcagcccg gtgagagagt cgaattgcgc gagacgctcg aggcgctctt ccgcgctcct
     1981 gcgctcgatc gcgaccgcca ggatgttcgc cacgctctgc aggaaacttg catcgtcctg
     2041 gccgaagtat cgctccgtgc gcgcgtgcgc gctgagtatt ccg
//
LOCUS       DASPZD010000126         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_668833, whole genome shotgun sequence.
ACCESSION   DASPZD010000126 DASPZD010000000
VERSION     DASPZD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668833"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VEM33_02440"
     CDS             <1..442
                     /locus_tag="VEM33_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50716.1"
                     /translation="LTHVPYKGAGPLMPALLGGEVDLAFDGMGTSATQIKAGKLRPLA
                     VTTKTRSPVIPDVPTMQEAGFSGYEVTTWYALWAIKGTPKEAIDRMYAETVKAMQQPD
                     MKQIWDAQGATIGGQTPAEFAKFVHGEIEKWGKVVRDAGIKIDL"
     gene            complement(486..1247)
                     /locus_tag="VEM33_02445"
     CDS             complement(486..1247)
                     /locus_tag="VEM33_02445"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HYS50717.1"
                     /translation="MRFPRWKTICAAAMCAPLLAIAQQQPSDYKREKRWADEIVPQLV
                     SGEPVWLEAPRTEKFLGIYTEVKNAKGAIILAHGLGVHPDHGLIGELRTRLADTGYTT
                     LSIQMPILSADAPPARYPVLFWEADARFAAAMTYLKRKRNDRIVLLSHSMGSRMANHY
                     VGAHPLVPLAGWISLSISSGDFAPIQRVKFPVYDVYAENDTDAVLKGAQKRALALKRM
                     RGSSQAMVFATDHFFAKKEKELVSLIGLLLEGAKK"
     gene            1342..>2243
                     /locus_tag="VEM33_02450"
     CDS             1342..>2243
                     /locus_tag="VEM33_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYS50718.1"
                     /translation="MAGPLSHIRVLDLSRVLAGPWAGQNLADLGAEVIKVERPGSGDD
                     SRAFGPPWVKDRNGRDTKDSAYYTSTNRGKKSITLNISRPEGQKLARELAKICDVLIE
                     NYKFGDLARYGLAYDDLKKTNPRLIYCSVTGFGQTGPYRERPGYDFMIQGMGGMMSVT
                     GERDDAPGGGPQRAGVPVADIITGMYASIAICAALAHRAESGAGQHLDLALLDSQIAL
                     LAYQNTNYFSTGSPPKRIGNLHPNIVPYQPFRTRDGSVILACGNDNLYGKFCEAAGCP
                     ELAADARFASNGKRVENRAELTRLL"
BASE COUNT          404 a          761 c          725 g          353 t
ORIGIN      
        1 gctcacgcac gtcccgtaca agggcgccgg accgctgatg ccggcgctcc tcgggggcga
       61 ggtcgacctc gccttcgacg gcatgggcac ctccgcaacg cagatcaagg caggaaagct
      121 gcgtcccctc gcggtgacga cgaagacgcg ctcgccggtg attcccgatg tgcccaccat
      181 gcaggaggcg ggcttcagcg gctacgaggt cacgacgtgg tacgcgctgt gggcgatcaa
      241 gggcacgccc aaggaggcga tcgaccgcat gtacgcggag acggtcaagg cgatgcagca
      301 acccgacatg aagcagatct gggacgcgca gggggcgacg atcggcgggc aaacaccggc
      361 ggagttcgcc aagttcgtgc acggcgaaat cgaaaaatgg ggcaaggtgg tgagggacgc
      421 cggaatcaag atcgacctct aggcgctgcc ggtcttgcgc agaagccccg cgcgagcggg
      481 gctttttatt tcttcgcgcc ttcgagaagc agcccgatca gcgatacgag ctctttttcc
      541 ttcttggcga aaaagtggtc ggtcgcgaac accatggcct ggctcgagcc gcgcatgcgc
      601 ttgagcgcca aggcgcgctt ctgcgcgcct ttcagcacgg cgtccgtgtc gttctccgcg
      661 tagacgtcgt agaccggaaa cttcacgcgc tggatcggtg cgaaatcccc gctcgagatg
      721 gaaagcgaga tccagcccgc gagcggaacc agcggatgcg cccctacata gtggttcgcc
      781 atgcgcgagc ccatgctgtg cgagagcagg acgatcctgt cgttgcgctt gcgtttcagg
      841 taagtcatcg ccgcggcaaa gcgcgcgtcc gcttcccaga agagcaccgg gtagcgcgca
      901 ggcggcgcgt ccgcggagag gatcggcatc tggatcgaaa gcgtcgtgta gcccgtgtcg
      961 gcgagccgcg tgcgcagctc gccgatcagg ccgtggtcgg gatgcacgcc caggccgtgc
     1021 gcgaggatga tcgcgccctt cgcgttcttc acctcggtgt agatgccgag gaatttctcg
     1081 gttcgcggcg cttccagcca caccggctct cccgagacga gctgcgggac gatctcgtcg
     1141 gcccagcgct tctcgcgctt gtagtcggaa ggctgctgct gcgcgatagc gagcagcggc
     1201 gcgcacatcg cggcggcgca gatcgtcttc caacgaggaa atcgcatccc gtgctccgtg
     1261 aaccccccgt tattgtagcg ttccgcgtcg ccggcacggg gctttcggat aaaataccgc
     1321 gtctccaacg gccgagaact catggcggga ccgctttcgc acatccgcgt cctcgatctt
     1381 tcgcgcgtgc tcgccggccc ctgggcgggg cagaacctcg ccgatctcgg cgccgaagtg
     1441 atcaaggtcg agcgtccggg ctcgggcgac gactcgcgcg ctttcggtcc gccctgggtg
     1501 aaggacagga acgggcgcga caccaaggac tcggcctact acacctcgac caaccgcggc
     1561 aagaaatcga tcacgctgaa catctcccgg cccgagggcc agaagctcgc gcgcgagctc
     1621 gcgaagatct gcgacgtgct catcgagaac tacaagttcg gcgatctcgc gcgctacggc
     1681 ctcgcctacg acgacctcaa gaaaaccaac ccgcgcctga tctattgctc ggtcaccgga
     1741 ttcggccaga ccgggccgta ccgcgagcgc cccggctacg acttcatgat ccagggcatg
     1801 ggcggcatga tgagcgtcac cggcgagcgc gacgacgcgc cgggcggcgg cccgcagcgc
     1861 gccggcgttc ccgtcgccga catcatcacc ggaatgtacg cttcgatcgc catctgcgcg
     1921 gcgctcgcgc accgggcgga gagcggcgcc gggcagcatc tcgatctcgc gctgctcgac
     1981 tcgcagatcg cgctcctcgc ctaccagaac accaactact tctccaccgg atcgccgccg
     2041 aagcggatcg gcaacctgca cccgaacatc gtgccctacc agccgttccg cacccgggac
     2101 ggcagcgtca tcctcgcctg cggaaacgac aacctctacg gcaaattctg cgaggccgcc
     2161 ggctgccccg agctcgccgc cgatgcgcgc ttcgcatcca acggcaagcg ggtcgagaac
     2221 cgcgcggagc tgacgcggct tct
//
LOCUS       DASPZD010000127         5922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_428862, whole genome shotgun sequence.
ACCESSION   DASPZD010000127 DASPZD010000000
VERSION     DASPZD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428862"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VEM33_02455"
     CDS             complement(<1..534)
                     /locus_tag="VEM33_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYS50719.1"
                     /translation="MVSTTRPDLSEIVESDVLNAGISDEDRSSLARALAFAADLYGGK
                     LLGTGEPAYEHAVGTARNVAELRLDADARAAGLLFAVPAYLPAAEETLAESFGAAVAS
                     LVMGISRLNALRVITRTAAQGKDSQSQADVLRKMLLAMVEDVRVVLLRLASRTQTLRW
                     LARTPNAERAQLARETLD"
     gene            608..1369
                     /locus_tag="VEM33_02460"
     CDS             608..1369
                     /locus_tag="VEM33_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HYS50720.1"
                     /translation="MGFFRDWRRKRVLRRARLDEALWERVASRLSYLRGLSKEEAARL
                     KELVVLFLAEKEMHGAQGFRLTDEVRLSIAAQACLPILNLGLDVYNGWVGVVVYPGEF
                     RVRKEEMDEHGIVHTFDEELAGEAWPGGPVILSWEDVGSAERGYNVVIHEFAHKIHMD
                     GAGDADFPAARAGMDAAEWRRVLEAAYERFCGEVDSGRPTFIDEYAAEHPAEFFAVMS
                     EAFFTESAVLARDWPELYRQFALFYRQDPAGRTGA"
     gene            1541..1933
                     /locus_tag="VEM33_02465"
     CDS             1541..1933
                     /locus_tag="VEM33_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYS50721.1"
                     /translation="MLEFSRRNDPWPFHVDEAAAARSPFGSIVASGGYIITLWYRSLI
                     EIYNTPRATWAFLGGLDWKVKFRAPVRAADTLRVRVTVKEKRLWIKPDRGLVTLFNEI
                     LNQRSEVVCAVEVMVLVATRAGGAAPGS"
     gene            complement(1955..2818)
                     /locus_tag="VEM33_02470"
     CDS             complement(1955..2818)
                     /locus_tag="VEM33_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50722.1"
                     /translation="MSEPFRRRKVQCASAGGLHGVAYLEWGERESSKTLVCVHGLTRC
                     ARDFDRLAAEMVRHGYRVVCPDVAGRGDSDWLENPMEYTVPTYVYDMVTLIARLDVES
                     VHWVGTSLGGLVGMSIAAMRGSPITRLVLNDVGPVLTAVSLERLSSYVGKWPPLPNIE
                     AAELFVRSTSESFGPHTDSEWRFLTEHVVRKNADGSLRMHYDPAIAVPFNAELSPKDV
                     ELWSFYDAISCPTLVLRGEQSDLLRRDTVEQMAVRGPRAKVVEIAGVGHAPTLLHDDQ
                     IAIVREFLLEQ"
     gene            complement(2815..3651)
                     /locus_tag="VEM33_02475"
     CDS             complement(2815..3651)
                     /locus_tag="VEM33_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50723.1"
                     /translation="MNLTATITIKGYPGLLREYRVHVNRLLDEEGGDSYRELHTGEQL
                     EYQFKLRGGIPFPPFVAASQAFPELTVEVGWDAADEGRSGHAVIQNGILREQAAQTHP
                     PAGATLQDVAADADGGLRLALACARWRGFWHGYVVASDQHAFFRIAGSPGSCRVSASD
                     GIEAEWAERWTVSAGDADYSELAPREPIAEDELRELDRLAQEFSREWIWFEESPLEET
                     AVERARFADCGYPVREANLRSEKLRKVLRPESGGLAFGSFGEDTRWIPELLRRCWLRP
                     AK"
     gene            complement(3672..4082)
                     /locus_tag="VEM33_02480"
     CDS             complement(3672..4082)
                     /locus_tag="VEM33_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50724.1"
                     /translation="MPVPGSENLFLGATLSSMQTDAGDSRVVNEAVHQIGTAAACGLI
                     TTAPLVPFYIEFTLNHGHDKSNTYVAVGACQMVSNDVPRPGIALAGCALRVTQGPAGF
                     LGGIATSMSIFNPLRLQGAGTGSFWTLRVYTTEN"
     gene            complement(4369..>5922)
                     /locus_tag="VEM33_02485"
     CDS             complement(4369..>5922)
                     /locus_tag="VEM33_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HYS50725.1"
                     /translation="VAGLLAMLVSWCWRRSETLSLALPAQKAAALAGFLAAFAYCLIS
                     GFAVPAQRTLYMVGVVAVALWFGRATSASRVLAAALFIVLALDPWAVLSPGFWLSFAA
                     VAVIFFVATGRTAKAHWLAQWGRVQWAVTAALAPLLLVLFQQVSLVSPVANALAIPLV
                     SFVITPLALAGAVLPFDWVLELGHAILDILMATLEWLATLPSAVWHQHAPLAWTVPLA
                     LAGIAWMLLPRGFPARWLGAPLVLPLFAVSPPSPGAGELWITVLDVGQGLAVLARTER
                     HALLYDAGPAFNASSDSGSRVILPYLRGEGIDGLDALVLSHDDRDHSGGAGSVLETMP
                     VGLLWSSFSPDHALLAAPAWRAPCVAGRKWLWDGVSFEFLHPSAEAAEGRAARANNRS
                     CVLRIEAPAGRVLLTGDIERTAERELLLRAPALLPADALLVPHHGSGTSSTPEFVRRV
                     APRHAVFAVGHRNRFGHPREDVLERYREAGSELLRTDTGGAIQLRFAPGGLRVYAERD
                     RARRYWHGL"
BASE COUNT         1040 a         1951 c         2040 g          891 t
ORIGIN      
        1 gtcgagcgtc tcgcgcgcga gctgcgctcg ctcggcattc ggggtgcgcg cgagccagcg
       61 cagcgtctgc gtgcggctcg caagccgcag cagcaccacg cggacgtcct ccaccatggc
      121 gagcagcatc ttgcgcagca catccgcctg cgactgcgag tccttgccct gcgccgccgt
      181 gcgcgtgatc acacgcagcg cgttgagccg gctgatcccc atgaccaggg aggcgaccgc
      241 cgcgccgaac gattccgcca gcgtttcctc ggcggcggga agataggcgg gtacggcgaa
      301 gaggagcccc gcggcacgcg cgtcggcgtc gagcctgagc tcggccacgt tgcgtgcagt
      361 ccccaccgcg tgctcatagg ccggctcgcc ggtgcccagc agctttccgc cgtagaggtc
      421 ggcagcgaag gccagcgcgc gggcgagcga gctccggtcc tcgtcggaga ttccggcgtt
      481 gaggacgtcg gactcgacga tttcggagag atccgggcgc gtggtggaaa ccatgctcgg
      541 agtttatccc acgccgtagc accgcagcgc cccgtcccgg ataatctgtc tttttccagg
      601 aaccctgatg ggcttcttcc gagattggcg ccgtaagcgc gtgctgagaa gggcccgcct
      661 cgacgaagcg ctgtgggaac gagtcgcctc gagactctcc tacctgcgcg gcctgtcgaa
      721 ggaggaagcg gcccggctga aggagctcgt cgtcctcttt ctcgccgaga aggaaatgca
      781 cggcgcgcag gggtttcgac tcaccgacga agtgcgcctg tcgatcgccg cccaggcctg
      841 cctgccgatt ctcaatctcg gcctggacgt ctacaacggc tgggtcggcg tcgtcgtcta
      901 tcccggcgaa ttccgcgtgc gcaaggagga aatggacgag cacggaatcg tgcacacctt
      961 cgacgaggaa ctcgccgggg aggcctggcc gggcgggccg gtgatcctct cctgggaaga
     1021 cgtgggatcg gccgagcgcg gctacaacgt cgtgatccac gaattcgcgc acaagatcca
     1081 catggatgga gcgggcgacg cggattttcc ggcggcacgc gccggaatgg acgcggcgga
     1141 atggcgcagg gtcctcgaag cggcctacga gcgtttttgc ggcgaggtcg actcgggtcg
     1201 accgaccttc atcgacgagt acgccgccga gcacccggcc gagttcttcg ccgtgatgag
     1261 cgaagccttc ttcaccgaat cggcggtgct cgcgcgcgac tggccggagc tataccgaca
     1321 gttcgcgctt ttctaccggc aggatccggc gggacgaacg ggcgcatagc ggcgcctcga
     1381 gcggcgttct ggtgcggcca tggccgttcg gctaagatca cagtactcaa ttggaagggg
     1441 ctgcccaacc cggagccaga gtatggcgac cgtctacttc gaagacctgg aaattgggtc
     1501 cgtgcatctc ggtcgggagt gtgtctgcga taaggatgag atgcttgagt tcagccggcg
     1561 caatgaccct tggcccttcc acgtcgacga agccgcagcc gcacgttcgc cattcggcag
     1621 catcgtggcg agcggaggct acatcatcac cttgtggtac cgctccctca tcgagatcta
     1681 caacactccg agggcaacct gggcattcct gggcggtctt gactggaagg tcaagttccg
     1741 cgctccggtt cgcgcggctg acaccctccg ggtgagagta accgtgaagg agaaacggct
     1801 ttggatcaaa cccgaccgag gcctggtcac actcttcaac gagatcctga atcaacgatc
     1861 cgaggtcgtg tgcgctgtcg aggtgatggt gctggtggcc actcgggcag gaggcgccgc
     1921 accaggctcg tgaactcaag tcaaacgtcg acttctactg ttccagcaga aactccctca
     1981 cgatcgcgat ctgatcgtcg tgaagcaggg tcggtgcgtg gccgacgccg gcgatttcca
     2041 cgactttcgc ccgcgggccg cgtaccgcca tctgctccac ggtgtcgcgc ctgagcaggt
     2101 ccgattgctc gccgcgcagg acaagggtcg ggcagctgat cgcgtcgtag aagctccaca
     2161 gctccacgtc cttcggcgac agctcggcgt tgaacggcac ggcaatcgcc gggtcgtagt
     2221 gcatgcgcag tgacccgtcg gcattctttc tgacgacatg ctcggtgagg aaacgccact
     2281 ccgagtcggt gtgcgggccg aaggactcgc tggtcgagcg cacgaacagc tcggccgctt
     2341 cgatgttcgg aagaggcggc cacttgccga cgtaggagct gaggcgctcg agcgacaccg
     2401 ccgtcagcac aggccccacg tcgttcagca ccagcctcgt gatgggcgaa ccgcgcatcg
     2461 ccgcgatgct catcccgacc agcccgccga gggaggtgcc gacccagtgc accgattcca
     2521 cgtccaggcg cgcgatcaga gtcaccatgt cgtacacgta ggtcggcacc gtgtactcca
     2581 tcggattctc gagccagtcg gaatcgccgc gcccggcgac gtcgggacag acgacgcggt
     2641 aaccgtggcg gaccatttca gcggccaggc ggtcgaaatc gcgcgcgcag cgggtgagcc
     2701 cgtgaacgca gaccagggtt ttgctgcttt cgcgctcgcc ccactcgagg taggcgacgc
     2761 cgtgcaggcc gccggcgctc gcgcactgca ccttgcggcg ccggaaaggt tcgctcattt
     2821 cgcgggtcgc agccagcagc gccgcaacag ttccggaatc cagcgcgtgt cttccccgaa
     2881 ggagccgaaa gcgaggccgc cgctttccgg acggagcacc ttgcgcagct tctccgagcg
     2941 caggttcgcc tcacgcaccg gatagccgca atcggcgaag cgcgcccgct cgaccgcggt
     3001 ctcctccagc gggctctcct cgaaccatat ccattcgcgc gagaactcct gcgcgagccg
     3061 gtcgagctcg cgcagctcgt cttccgcgat cggttcgcgg ggcgcaagct ccgagtagtc
     3121 cgcatcgcct gcggagaccg tccagcgctc cgcccattcc gcctctatcc cgtcggaagc
     3181 ggaaacccgg caggacccgg gcgatccagc gatgcggaaa aaagcgtgct ggtcggaagc
     3241 gacgacgtag ccgtgccaga agccgcgcca gcgcgcgcac gcgagggcga gccgaagccc
     3301 gccgtccgcg tcggctgcga cgtcctgcag ggtcgcgccg gcgggcgggt gcgtctgcgc
     3361 agcttgctcg cgcaggatgc cgttctggat gaccgcgtgg ccgcttcggc cctcgtccgc
     3421 agcgtcccag ccgacctcga cggtcagctc cggaaacgcc tgcgacgccg cgacgaaagg
     3481 cggaaaggga atacctccgc gcagcttgaa ttggtactcg agctgctccc cggtgtgcag
     3541 ctcgcggtag gaatcgccgc cttcttcatc gaggaggcgg ttgacgtgaa cgcggtattc
     3601 ccggagcaac ccgggatagc cctttatcgt gatcgtcgca gtcaggttca agccgagcag
     3661 gacgacccgg gtcagttctc cgtcgtgtag acgcggagcg tccagaacga cccggtgccg
     3721 gcgccttgca gccgaagcgg gttgaagatg ctcatgctcg tcgcgatgcc gcccaggaag
     3781 cccgctgggc cttgcgtcac gcgcagcgcg catcccgcga gcgcaatacc gggcctcggg
     3841 acatcgttgg agaccatctg gcaggcgccg accgcgacgt aggtattgct cttgtcgtgc
     3901 ccgtggttga gcgtgaactc gatatagaaa ggcacgagcg gcgcagtcgt gatcaggcca
     3961 cacgccgccg cggtgccgat ctgatgcacg gcctcgttga cgacgcggct gtccccggcg
     4021 tccgtctgca tcgacgagag ggtggcgccc aggaagagat tttcgctccc gggaacgggc
     4081 acgcattggg gtggctggag acatttgggt cctcgtggct gcggaagacg agggtgtgct
     4141 ggccgggctc gcccgcgggt ggtccttcct gtgcggcggc gggtccggcg acgaggagga
     4201 cagcaagggt cgcgagagca gaggcgcgca atcggaacct catattggtt ttgcttctgg
     4261 attcgactga tgagaaaatt caatctacgc gccgacaacg cgccgtgcgg atcatgcact
     4321 cgcaaggcga gccaggtcaa gcactggcgc gctcgcgagt ctagccgctc acaacccgtg
     4381 ccagtagcgc ctcgcgcgat cgcgctcggc gtagacgcgc aggcccccgg gcgcaaagcg
     4441 cagctggatc gctccgccgg tgtcggtgcg gagcagctcg ctgccggcct cgcggtagcg
     4501 ctcgagcacg tcctcgcgcg gatggccgaa gcggttgcga tggccgacgg cgaacaccgc
     4561 gtggcgcggc gccacccgcc tcacgaactc cggcgtcgag gaggtgccgc tgccgtgatg
     4621 ggggacgagc agtgcgtccg cgggcagcag cgcgggcgcg cgcagcagaa gctcgcgctc
     4681 tgcagtgcgc tcgatgtctc cggtgagcag aacgcggccc gcgggggcct cgatccgcaa
     4741 tacgcagctg cggttgtttg ctcgcgctgc acggccttca gccgcctccg cggacgggtg
     4801 gaggaattcg aaggagactc catcccagag ccatttgcgc ccggcgacgc agggcgcgcg
     4861 ccacgccggg gccgcaagca gggcgtgatc cggggaaaac gacgaccaga gcaggccgac
     4921 aggcatcgtc tcgagcaccg aacccgcgcc gcccgaatgg tcgcggtcgt catgggaaag
     4981 aacgagcgcg tcgagaccat cgatgccttc gccgcggaga taggggagga tcacgcggct
     5041 tccgctgtcg gaagacgcgt tgaacgccgg gccggcatcg tagagcagcg cgtgtctttc
     5101 cgtgcgcgcg agcacggcga gcccctgccc cacatcgagc acggtgatcc agagctcgcc
     5161 cgcaccgggt gacggcggcg agaccgcgaa aagcggcagc accaagggcg ctccgagcca
     5221 acgcgcgggg aacccgcgcg ggagcagcat ccaggcgatc ccggcgagcg cgagcggcac
     5281 ggtccacgcg agaggcgcgt gctgatgcca gaccgcgctc gggagcgtgg ccagccactc
     5341 gagggtcgcc atcaggatat cgaggatcgc gtgccccagc tcgagaaccc aatcgaacgg
     5401 cagcaccgcg ccggcgagcg cgagcggggt gatgacgaag ctcaccagcg ggatcgcgag
     5461 cgcgttggcg accggagaga cgagcgacac ctgctggaac agcacgagta gcaggggagc
     5521 gagggccgcg gtgaccgccc attgcaccct tccccactgc gcgagccagt gggccttggc
     5581 ggtgcggccg gtagccacga agaagatcac cgcgaccgcg gcgaacgaca gccagaagcc
     5641 cggcgacagg accgcccagg gatcgagcgc gaggacgatg aagagcgccg cggcgagcac
     5701 gcgcgaggcg ctcgtcgccc ggccgaacca gagcgcgact gcgaccacgc cgaccatgta
     5761 aagagtgcgc tgcgcgggaa ccgcgaatcc ggaaatgagg caataggcga acgcggcgag
     5821 aaaaccggcg agcgccgctg ctttctgcgc gggcaatgcg agcgagaggg tctcgctgcg
     5881 ccgccagcac caggacacga gcatcgcaag caggcccgcg ac
//
LOCUS       DASPZD010000128         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1512567, whole genome shotgun sequence.
ACCESSION   DASPZD010000128 DASPZD010000000
VERSION     DASPZD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512567"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1281
                     /locus_tag="VEM33_02490"
     CDS             160..1281
                     /locus_tag="VEM33_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYS50726.1"
                     /translation="MLDVLGHPERHFPTIHIGGTNGKGSTCAFVANELRAQGWRVGVY
                     ASPHLISPCERITVDGVPIGEDAFAEWTAQLKLQIERVDASFFEATTAIAFADLAARD
                     VDIGVIEVGLGGRLDATNVLTPLVTAVTKIALEHVEYLGSDLKGIAREKAGIAKPKVP
                     FITGEADPDIRAELVREAKRRGAEPIITVDTTLRPRKDTKLGLRGKHQIANALVAHAI
                     LNALPKPFGPVGKSLPASFAHAYIPGRFEVRGKWIFDVAHNPDGMRVVAQALAESGRT
                     IRRPLHALVGIRNDKDWRAMLNSLRPLVDRLTLTVPPSIPLMQRWSIEEMGPDVSFEP
                     DFERALYDIQEGAETILVTGSFHTVGDAMARLPGFAPVG"
     gene            1291..2538
                     /gene="hisS"
                     /locus_tag="VEM33_02495"
     CDS             1291..2538
                     /gene="hisS"
                     /locus_tag="VEM33_02495"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYS50727.1"
                     /translation="MQGKALPGFRDFYPEDLTLRNHIFATWRSVAQRYGFEEYDGPPL
                     ESLDLYTQKSGDEIVGQLYSFKDKGDRDVALRPEMTPTLARMVAARGQALKKPIRWYS
                     IPQLFRYERQQKGRLKEHFQLNMDIIGEAAFLADAELMAAAVDIMRALGLGAGDVRLR
                     VSDRRLINALLTSRGLTEGQLPTAYAVIDRSERVPRQVLEEMLRDAKFSKKEASAVFE
                     VAALRGKDALAEGGQSAEHLTGAVRALEAMGLGDFVSIDLTIVRGLAYYTGIVWELFD
                     ASKSLRAIAGGGRYDSLLDLPALGFGMGDVVLGELLKERAVAPKASIELGAFLIAVGG
                     EDLPTVLQLAHALRERGISVEYGLRHAGVRKQLELASARGAARAVIVGPEERAEGLAV
                     VRDLRTSTEAKVPIRQLLNGYFD"
BASE COUNT          514 a          833 c          870 g          474 t
ORIGIN      
        1 tccccgtggc gaattgcggg atacgagcgc ccagctgctg cgtcacatga ccggcgcgtg
       61 gccggctgag gccgccgact gaccttcgaa gacgccgttc agtttctttt cgctcgacaa
      121 gccggtcgta tcaagtggag ccttgggccg accgagggct tgctcgacgt gctcggccat
      181 ccggagcgtc attttcccac gattcacatc ggtggcacga acggcaaagg gtccacctgc
      241 gccttcgtgg ccaacgagct ccgggcacaa gggtggcgcg tcggagtgta tgcctccccc
      301 catctcatat ctccctgtga acggattacg gtcgacggtg tcccgatcgg ggaggacgcg
      361 ttcgccgaat ggacggcgca gctcaagctg cagatcgagc gggtcgacgc gagcttcttc
      421 gaagcgacca cggccatcgc tttcgcggat ctcgcagctc gggacgtcga catcggtgtc
      481 atcgaagtag ggctgggcgg ccggctcgac gccaccaacg tgctgacacc gctcgtcacg
      541 gcagtcacga agatcgcgct ggagcacgtc gagtacctgg gcagcgacct caaggggatc
      601 gcgcgcgaaa aggcgggaat cgcgaaaccg aaggtcccgt tcatcaccgg cgaggccgat
      661 cccgacatcc gagccgagct cgtgcgggag gccaaacgcc ggggcgccga gccgatcatc
      721 acggtcgata cgacgctgcg gccgcgcaag gacacaaagc tggggctgcg agggaagcat
      781 caaatcgcca acgcactcgt tgcgcacgcc attctcaacg cgctaccgaa gccattcggg
      841 ccggtgggga aaagcctgcc cgccagcttt gcccacgcct acatccccgg ccgctttgaa
      901 gttcgcggca agtggatctt cgacgtggcg cataatccgg acggcatgcg cgtcgtcgct
      961 caggcgctgg ccgaatcggg caggacgatc cgccgaccgc tgcatgcact ggtcgggatt
     1021 cgcaatgaca aggattggcg ggcgatgctc aattcgctgc ggccattggt cgaccgcctc
     1081 acgctgaccg tccccccatc gattccgctc atgcaacgct ggtcgatcga ggagatgggg
     1141 cccgacgttt cgttcgagcc ggattttgag cgcgcactgt acgacattca ggagggagcg
     1201 gagaccattc tagtgaccgg ttcgtttcat accgtcggcg acgcgatggc tcgcttgccc
     1261 ggcttcgcgc ccgtcggtta agttgttgcg atgcaaggaa aagccctccc cggctttcgc
     1321 gacttttacc ctgaagacct gaccctccga aaccacatct ttgcgacctg gcgcagtgta
     1381 gcccagcgat atggcttcga ggagtacgac gggcctccgc tcgagtcact cgacctatac
     1441 acccagaaga gtggcgacga gatcgtcggt cagctctaca gtttcaagga caaaggggac
     1501 cgcgacgtcg cgctgcggcc cgagatgacg ccgacgctgg cgcgcatggt agctgcgcga
     1561 ggacaggcgt tgaagaaacc aatacgctgg tactcaattc cccaactgtt tcgttacgag
     1621 cgtcaacaga aagggcgact gaaagaacat tttcagttga atatggacat catcggcgag
     1681 gctgccttcc tcgcagacgc cgaactcatg gcggcggcgg tggacattat gagggccttg
     1741 gggttggggg caggggatgt gaggctcagg gtctcagacc gccggctgat caacgcgtta
     1801 ctcaccagtc gcgggctgac ggaagggcaa ctcccgaccg cgtatgcggt catcgatcgt
     1861 agcgaacgcg tgccgcgaca agtgctggag gaaatgctca gggatgccaa gttcagcaaa
     1921 aaggaagcgt ctgccgtttt cgaggtcgca gcgctgcgcg gcaaagacgc gctcgccgag
     1981 ggcggccagt ccgccgagca cctcaccggg gccgtgcgcg ccctcgaggc catgggtctt
     2041 ggggacttcg tttcgatcga tctgacaatc gtgcgaggtc tcgcctatta caccggcatc
     2101 gtgtgggagc tattcgacgc cagcaagtca ttgcgcgcga ttgcgggggg agggcgctac
     2161 gacagcctcc tcgatcttcc cgccctgggg ttcggcatgg gagacgttgt actcggcgag
     2221 ctcctcaagg agcgcgccgt agccccgaag gcgtcgatcg agctcggcgc ctttttgata
     2281 gcggtcggcg gggaagatct acccaccgtt ctgcagcttg cgcacgcgct gcgggaacgg
     2341 ggaatctcgg tcgaatacgg tctccggcat gccggcgtga gaaagcagct cgagctggcc
     2401 tcggcacggg gcgccgcccg cgcagtcatc gtcggtcctg aggagcgcgc cgagggcctg
     2461 gcggtcgtgc gcgatctccg gacgagcacc gaagcgaaag tacccattcg ccagcttctc
     2521 aacggatatt tcgattaatg gcacaagcac agaacgcgaa agcgcagcga atcacaccac
     2581 gcgccaaaga cttcagcgag tggtacggcg acgtcatcgc ccaggcgcag ctggcggact
     2641 attcgcccgt gcgcggctgc atggtgattc gtccccacgg ctatcggatc t
//
LOCUS       DASPZD010000129         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_217806, whole genome shotgun sequence.
ACCESSION   DASPZD010000129 DASPZD010000000
VERSION     DASPZD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217806"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VEM33_02500"
     CDS             complement(<1..735)
                     /locus_tag="VEM33_02500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYS50728.1"
                     /translation="MAVLKQLGKYKVVEVIGKGAMGVVYKGYDPDLERHVALKTVRKE
                     LVDENLAAQIIARFKNEALASGRLNHPAIVGIYEYGENKQLAYIAMEYVQGRGLRDFL
                     ARQERFGLQDVMSIMSRLLDALEYAHEHGVVHRDIKPQNIIMTPDGRLKLADFGIARI
                     DRSNLTQVGSIMGTPAYMSPEQYAGQQVDRRSDIFSCGVVLYELLTGVKPFEGPTETV
                     GYKICHEPHRNPSEINPQGVPEVFDAI"
     gene            complement(766..1638)
                     /locus_tag="VEM33_02505"
     CDS             complement(766..1638)
                     /locus_tag="VEM33_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HYS50729.1"
                     /translation="MRLIVKFNMVFLAIFLVGLAIAAYVSLDLLRQNARDEVLQHARI
                     MMESALAIRGYTSKQVRPLLETQIKYQFLPQSVPAYAANEQFSDLRKKFADYDYKEAT
                     LNPTNPRNRATDWETDVVNQFRQAPDRVEIVGERDTPTGRALYMARPLQIKDAACLYC
                     HSTVDAAPKTMIELYGPANGFGWKLNEVVGAQIVSVPMTLPIKRANDTFKVFMISLSG
                     VFAFIFVALNLMLHAIVIRPVTRLSRIADEVSLGNLDAPEFASKGKDEIATLAGSFNR
                     MRTSLVQAMKMLGE"
     gene            complement(1650..2600)
                     /locus_tag="VEM33_02510"
     CDS             complement(1650..2600)
                     /locus_tag="VEM33_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HYS50730.1"
                     /translation="MRSRSYGSEVGGDMRGCGRGVLGPCFQLAAALLGACASAFAQTA
                     TLTLAEGSVELIRGATLYTVTQGVRLGDGDILSIDPKGQAQIEFQDGAILNLSQGARA
                     MLTTIASGARGQSEIALLSGWAKFTQKKSGKGTQYRYLTPRVEINAGEATAVLNTGDG
                     STDIYVESGAVRFSEIGRKGVQRIARDAKGGEFIVRRGEQPATVGSRPSAEFIKSMPR
                     HFRDDLPALLDRFKNRRSEPKRDHEVTYAEVEAWLKAGSAIRRNLVKRFEIRSKDSEF
                     RRKLIENLREHPEWDKVLFPEKYAPKDPGDPKSARPGTQR"
BASE COUNT          452 a          839 c          845 g          576 t
ORIGIN      
        1 gattgcatcg aagacctcgg gaacgccctg gggattgatc tccgaaggat tgcggtgggg
       61 ttcgtggcat atcttgtacc cgacggtctc ggtcgggcct tcgaacggct tcacgccggt
      121 gagcagctcg tagaggacga caccgcagga aaagatgtcg ctgcgccggt caacctgctg
      181 ccctgcatat tgctccggtg acatgtacgc gggcgtgccc atgatggagc cgacctgggt
      241 gaggttggag cggtcgatcc gggcaatccc gaagtctgca agcttgagac ggccgtcagg
      301 cgtcatgatg atgttctggg gcttgatatc gcggtggact acgccgtgct cgtgggcgta
      361 ctcgagagcg tccagcaacc ggctcatgat gctcatcacg tcctgcaggc caaaacgctc
      421 ctgccgggcg agaaaatccc gcagcccgcg gccctgaaca tactccatcg cgatataggc
      481 gagctgcttg ttctcgccgt attcgtagat gccgacgatc gcaggatggt tgaggcggcc
      541 cgaggcgagc gcctcgttct tgaatctcgc aatgatctgc gccgcgaggt tttcgtcgac
      601 cagctccttg cgcacggtct tcagcgcgac gtggcgctcg agatcggggt cgtatccctt
      661 gtagacgacc cccatcgccc ccttgccgat gacttcgacg actttgtact tgccgagttg
      721 cttgagaact gccactagtc tccgggaccg ccgttatggc ggccttcact ccccgagcat
      781 cttcatcgct tgcaccaggc tcgttctcat gcggttgaac gatcctgcga gagtcgcgat
      841 ctcgtccttg cccttgctcg cgaattccgg ggcgtccaga ttgccgaggc tgacctcgtc
      901 ggcgatccgc gacagccgtg tgacgggccg gatcacgatg gcatgaagca tgaggttgag
      961 ggcgacgaag atgaaggcga agaccccgct gagcgagatc atgaatacct tgaaggtgtc
     1021 gttggcgcgc ttgatgggca aggtcatggg gacggacacg atctgcgccc cgactacctc
     1081 gttcagcttc cagccgaagc cgttcgccgg accgtagagt tcgatcatgg tcttgggcgc
     1141 tgcgtccacg gtgctgtggc aatacaggca ggctgcgtcc ttgatctgca ggggccgggc
     1201 catgtacagg gcacggccgg tcggtgtgtc gcgctcgccg acgatctcga cgcggtccgg
     1261 cgcctggcgg aactggttca cgacgtcggt ttcccaatcg gtggcgcggt tgcggggatt
     1321 ggtcggattg agcgtcgctt ccttgtagtc gtaatcggcg aatttcttgc gcaggtcgct
     1381 gaactgctcg ttggcggcgt acgcgggaac cgactgcggc aggaactggt acttaatctg
     1441 agtctcgagc agcgggcgca cctgcttgga ggtgtagccg cgtatcgcga gcgccgattc
     1501 catcatgata cgggcgtgct ggagcacctc gtcgcgcgcg ttctgccgca gcaggtcgag
     1561 cgatacatag gcggcgatgg ccagaccgac caggaagatt gccagaaaca ccatgttgaa
     1621 cttgacgatc agtctcatga ccttccctcc tagcgttgcg tgccgggacg cgcgctcttc
     1681 gggtcccccg ggtccttcgg agcgtacttt tccgggaaca gaactttgtc ccactccggg
     1741 tgttcccgga gattctcgat cagcttgcgg cgaaactcgg aatccttgga tcggatctca
     1801 aatcgtttga cgagatttct tctgattgcg gaaccggctt taagccaggc ttcgacctcg
     1861 gcgtacgtca cctcgtgatc gcgcttgggc tccgacctcc tgttcttgaa ccggtcgagc
     1921 aacgccggca ggtcgtcccg aaaatgccgc ggcatgcttt ttatgaactc cgccgagggg
     1981 cgcgagccga cggttgccgg ctgctcgccc ctgcggacga tgaactcgcc tcccttcgcg
     2041 tcgcgcgcga ttctctgcac gcctttcctg ccgatttccg aaaatctcac ggcgccggat
     2101 tcgacgtata tgtcggtgga gccgtcgccc gtgttcagaa ccgcggtcgc ctcccccgca
     2161 ttgatctcga cgcgcggcgt gaggtagcgg tactgtgtgc ctttgccgga ttttttctgg
     2221 gtgaactttg cccagccgga cagcagcgcg atctccgact ggccgcgcgc tcccgacgcg
     2281 atggtcgtca gcatggcgcg cgcgccctgg ctgagattga ggatggcgcc gtcctggaac
     2341 tctatttgcg cctgtccctt ggggtcgatc gacaatatgt ctccgtctcc cagcctcacg
     2401 ccctgggtga cagtgtagag cgtcgcgccc cgtatcagct cgacgctccc ttcggccagc
     2461 gtcagggtcg cggtttgagc aaaagcggaa gcgcaggcgc ccaataaggc cgctgcaagc
     2521 tggaaacagg gtcccagcac cccccggccg catccgcgca tgtccccccc gacttcgctt
     2581 ccgtagcttc tggagcgcat tctagagcag acggcaatga gcgctcaagt gcgacccagg
     2641 ccggaactcc acactaagct gctcgccttt tgccttatac tttgcgccgc caggatggtc
     2701 cgcacctgtc cg
//
LOCUS       DASPZD010000130         4872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1750809, whole genome shotgun sequence.
ACCESSION   DASPZD010000130 DASPZD010000000
VERSION     DASPZD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750809"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VEM33_02515"
     CDS             <1..930
                     /locus_tag="VEM33_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYS50731.1"
                     /translation="MARLDSRLVGITPAMREGSGLVRFAEEFPDRYFDVGIAEQHAVT
                     FAAGLACEGLKPVLAIYSTFLQRGYDQLIHDVCIQNLPIVFALDRAGLVGGAGATHNG
                     AFDLTYLRCVPNMVVMTPSDENECRQMLATAFSLDQPAAVRYPRGSGPGAGIVKEMSR
                     LPLGKGIVRRRAPGGPGSVAILAFGSLVKPALEAGADLDATVVDMRFVKPVDAELAAE
                     LAATHSLLVTIEENAVLGGAGSGVAEALTARAIVTPLLQLGLPDRFVDHGDQAQLLAS
                     VGLDKAGIVAAVRARLEKVGTERMLKSSNTEPT"
     gene            944..1750
                     /gene="folE2"
                     /locus_tag="VEM33_02520"
     CDS             944..1750
                     /gene="folE2"
                     /locus_tag="VEM33_02520"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HYS50732.1"
                     /translation="MNIRDPLVIPDVQGSEDRRKLAIDQVGIKAIRHPIRVAERSGDV
                     QHTVATFNMYVGLPHQFKGTHMSRFVEILNAHEREISVETFKLMLDEMVERLEARSGH
                     IEMTFPYFVNKAAPISKVKSLMDYEVTFVGEIDKGEKCFKMKVLVPVTSLCPCSKRIS
                     DYGAHNQRSHVTITARTKGFVWIEELVDYAEKHASSELYGLLKRPDEKFVTERAYDNP
                     RFVEDMVRDIAAELNRDKRIEWYRVESENFESIHNHSAYALIEKDKTNKQ"
     gene            complement(1871..2440)
                     /locus_tag="VEM33_02525"
     CDS             complement(1871..2440)
                     /locus_tag="VEM33_02525"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HYS50733.1"
                     /translation="MIGLFPGKAVVVIDGGAPRVLSVGQKPVDGVTLISTDRESATLV
                     IDGQKKTLRIGQHQGGQAPASTSQSVTLTADPRGHFVVDGQVNGGAVRFLVDTGATAI
                     SLSSADATRLGIDYRKGQPGLMGTANGTLVVYRVKLDTVRVGDILVNNVDGAVLEGNP
                     MPFALLGMSFLNRMEMKREGQTMVLIRRF"
     gene            2531..3235
                     /locus_tag="VEM33_02530"
     CDS             2531..3235
                     /locus_tag="VEM33_02530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HYS50734.1"
                     /translation="MKPVAIFRHSQTEGPGYFATFLDAHRIPWRLVKVDAGEPVPADP
                     REFSGLAFMGGPMSVNDELPWIPPVLGLVNEAVDAEVPVLGHCLGGQLMAKALGGRVT
                     RNGVKEIGWGPVTIEDNARAREWFGARREFWGFHWHGETFTIPPRATRIAGSLYCANQ
                     AFVLGKHLGMQCHVEMTPELIRAWCEDWEKELVAKAGPSVQTPQQMFERLDERVHSLN
                     TIAADLYGRWIEGLKK"
     gene            3247..4416
                     /locus_tag="VEM33_02535"
     CDS             3247..4416
                     /locus_tag="VEM33_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYS50735.1"
                     /translation="MPKAKATKGTPRPASGPVTLLVGTRKGAFFIRSDRARRSWMLAG
                     PHFLGNIVNHLVLDPRDRKTLLCAVRAGHLGPTVFRSTDSGRTWKEAERPPAFPKAAA
                     GEKGLAVEHVFWLTPGHSSEPGAWYAGVSPPGLFRSDDGGVTWEGVAGFNANPMRIVW
                     IGGDQEQGPPGGATLHSINVDPRDPRHLYIGISVAGAFESSDRGETWRPLNQGVAADF
                     LPAKDPEYGHDVHSMRLHPLAPDRLYQQNHCGIYRLDRPGEKWERIGNAMPKAIGDIG
                     FPVVLHPRDPEALWVFPMDGGTVWPRVSPEGKPAAYVSRDGGRTWRRQDTGLPEKQGW
                     FTVKRQAMCADAHDPVGVYFGTTSGEVWASANEGDRWTSIARHLPEIYSVEAAGG"
     gene            4418..4678
                     /locus_tag="VEM33_02540"
     CDS             4418..4678
                     /locus_tag="VEM33_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYS50736.1"
                     /translation="MKVLIPSQLRDYTGGRAHVEAHGATLAALLADLERTYPGIRFRM
                     IDEQDAIRRHIKIFVNQQNTRDLSAALAADDEVVIVGALSGG"
     gene            complement(4675..>4872)
                     /locus_tag="VEM33_02545"
     CDS             complement(4675..>4872)
                     /locus_tag="VEM33_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HYS50737.1"
                     /translation="KAGIEQEKAKSIVKLLKDSKMKVQASIQGTAVRVSGAKRDDLQA
                     AIALVRKSVTDIPLQFQNFRD"
BASE COUNT          874 a         1613 c         1573 g          812 t
ORIGIN      
        1 atggcgcggc tcgattcgcg tcttgtcggc atcaccccgg cgatgcggga gggctcgggg
       61 ctcgtgcgtt ttgctgagga atttcccgac cggtatttcg acgtgggcat cgcggagcag
      121 cacgcggtca ctttcgccgc gggactggcc tgcgagggcc tcaagcccgt gctcgcgatc
      181 tactcgactt ttttgcagcg cggctacgac cagctcatcc acgacgtctg catccagaac
      241 cttccgatcg tgttcgcgct cgaccgggcg ggcctcgtgg gcggcgccgg tgccacgcac
      301 aacggcgcct tcgacctgac gtatctgcgc tgcgttccca acatggtggt gatgacgccg
      361 agcgacgaga acgaatgccg ccagatgctc gccaccgcgt tctcgctcga tcagcccgcc
      421 gcggtgcgct atccccgcgg cagcggcccg ggtgcaggga tcgtgaagga aatgagccgg
      481 ctgccgctcg gcaagggaat cgtgcgccga cgtgctcccg gcgggccggg ctccgtcgcg
      541 attctcgcgt tcggcagcct ggtcaagccc gcgctcgagg cgggcgccga cctcgatgcc
      601 accgtggtcg acatgcgctt cgtgaagccg gtggacgccg agctcgcggc cgagctcgcg
      661 gcgactcact cactcctcgt caccatcgag gagaacgcgg tcttgggcgg cgcaggaagc
      721 ggcgtcgccg aagcgcttac cgcgcgcgcc atcgtcaccc cgctcctcca gctggggctt
      781 cccgaccgct tcgtcgatca cggcgaccag gcccaattgc tcgcttcggt gggcctggac
      841 aaggcgggca tcgtcgccgc cgtccgcgcg cggcttgaaa aagtgggaac ggaacgcatg
      901 ttaaaatcct ctaacactga gcccacgtga cgaaaggctc ttcatgaata tccgcgatcc
      961 gcttgtcatt cccgacgtgc aaggctccga ggacaggcgc aagctggcca tcgaccaggt
     1021 cggcatcaag gccatccgcc atcccatccg cgtcgccgaa cgctcgggcg acgtgcaaca
     1081 taccgtcgcg acgttcaaca tgtacgtcgg cctgccgcac cagttcaagg gaacgcacat
     1141 gtcgcgcttc gtcgagatcc tgaacgcgca cgagcgcgag atctccgtgg agacgttcaa
     1201 gctgatgctg gacgagatgg tcgagcgcct ggaagcccgg tccggccaca tagagatgac
     1261 gtttccttac ttcgtcaaca aggccgctcc gatctccaag gtcaaaagcc tgatggacta
     1321 cgaagtgacg ttcgtcgggg agatcgacaa gggtgagaag tgcttcaaga tgaaggtcct
     1381 cgtaccggtg acgagccttt gtccgtgctc caagagaatc tccgattacg gagcgcacaa
     1441 tcagcgctcg cacgtgacga tcaccgcgcg caccaagggg ttcgtgtgga tcgaggagct
     1501 ggtcgactac gccgagaagc acgcctcgag cgagctctac gggttgctga agcgccccga
     1561 cgagaagttc gtcaccgagc gcgcctacga caaccccagg ttcgtcgagg acatggtgcg
     1621 cgacatcgcc gcggagctga accgcgacaa gcgcatcgag tggtaccggg tggagtcgga
     1681 gaatttcgag tccatccata atcattccgc ctatgcgctc atcgagaagg acaagaccaa
     1741 caagcagtaa gtaacgaaca ggggaaccct acggttcccc ttgggctccg cccaagggct
     1801 cataaacgag cggggaaacc ctacggtttc cccgctaccc cttccccaac cgaaagccag
     1861 atagaccccg ctagaaccgc cggatcagca ccatcgtctg gccttcgcgc ttcatttcca
     1921 tgcggttcag gaagctcatc ccgagcagcg cgaacggcat cgggttgcct tccaggacgg
     1981 ctccatcgac gttgttgacg aggatgtcgc cgacgcgcac cgtgtctagc ttcacccggt
     2041 agaccacaag ggtgccgttg gcggtgccca tcaatccggg ctgccctttg cggtagtcga
     2101 ttccgaggcg cgtcgcgtcc gcagaagaga gcgagatcgc cgtggcgccc gtgtcgacca
     2161 gaaagcgcac ggcgccgccg ttgacctgcc cgtccacgac gaaatgtccg cgcgggtcgg
     2221 cggtgagcgt gacgctctgg ctcgtggaag ccggagcctg acctccctgg tgctgtccga
     2281 tcctcagggt ctttttctgg ccgtcgatga ccagggtcgc ggactcccgg tcggtcgaga
     2341 tcagcgtgac gccgtccacg ggtttctggc ccacggaaag aacgcgcggc gcgccgccgt
     2401 cgatcaccac gacggccttg ccggggaaga ggccgatcac gctcacctcg gcggtccagc
     2461 cggcagagga caacaggagg agcagcgacg ctacaataca gggcttgacg acgttggcct
     2521 cgtttcgcgc atgaaaccag ttgcgatttt ccgacatagc cagaccgagg ggccgggcta
     2581 ttttgccaca ttcctcgacg cgcaccgcat tccgtggcgg ctcgtcaaag tggacgcggg
     2641 agagccggtc cccgcggatc cgcgcgaatt ctccggtctc gcattcatgg gcgggccgat
     2701 gagcgtgaac gacgagctgc cgtggatccc tccggtgctc gggctcgtga acgaggcggt
     2761 cgacgcggag gttccggtgc tcggccactg cctcggtgga cagctcatgg caaaggcgtt
     2821 gggcgggaga gtcacgcgca acggcgtcaa ggaaatcggc tggggcccgg tcactatcga
     2881 ggacaacgcc cgcgcgcgcg agtggttcgg cgcgaggcgg gagttctggg gctttcactg
     2941 gcacggcgag actttcacca tcccgcccag ggcgacgcgg attgccggaa gtctgtattg
     3001 cgcgaaccag gcgttcgtgc tcggcaagca cctcggcatg caatgccatg tcgagatgac
     3061 gcccgagctg atccgcgcct ggtgcgagga ttgggaaaag gagctcgtcg cgaaagccgg
     3121 gccgtcggtg cagaccccgc agcagatgtt cgagcggctc gacgagcgcg tgcattcgct
     3181 caacacgatc gccgcggacc tctacggacg ctggatcgaa ggattgaaaa aatagagagg
     3241 ttgcttatgc cgaaagccaa ggcaacaaag ggaaccccgc gtcctgcttc ggggccggtc
     3301 acgctcctcg tgggaacgcg caaaggcgcg tttttcatcc gttcggatcg cgcgcgccgc
     3361 agctggatgc tcgcgggtcc ccactttctc ggcaacatcg tcaaccatct ggtgctcgac
     3421 ccgcgcgacc gcaagacgct gctgtgcgcg gtgcgcgcgg gccacctcgg gccgaccgtg
     3481 ttccgctcga ccgattcggg ccgcacgtgg aaggaagccg agcgcccgcc cgcgttcccg
     3541 aaggccgccg ccggcgagaa aggcctcgcg gtcgagcacg tgttctggct tacgccgggg
     3601 cattcctcag aacccggcgc ctggtacgcg ggcgtctcgc cgccgggatt gttccgctcc
     3661 gatgacggcg gggtcacctg ggaaggcgtc gccggcttca atgccaaccc catgcgcatc
     3721 gtctggatcg gcggcgatca ggagcagggg ccgcccgggg gcgcaacgct gcactcgatc
     3781 aacgttgatc cgcgcgaccc ccggcatctc tacatcggca tctcggtcgc cggcgcattc
     3841 gagagctcgg accgcggaga aacctggcgc ccgctcaatc agggcgtcgc ggccgatttt
     3901 ctcccggcga aagacccgga gtacggacac gacgtgcaca gcatgcggct gcatccgctc
     3961 gcacccgacc gcctgtacca gcagaaccac tgcggcattt accgtctgga tcgtcccggc
     4021 gaaaaatggg agcgcatcgg caacgccatg ccgaaggcga tcggcgacat cggatttccg
     4081 gtcgtgctgc atccgcgcga tcccgaggcg ctgtgggtgt tcccgatgga cggcggcacc
     4141 gtgtggccgc gcgtgagtcc ggagggcaag cccgcggcct acgtatcgcg cgacggcggc
     4201 cggacctggc gccgccagga cacaggtctc ccggaaaaac aaggctggtt taccgtgaaa
     4261 cgccaggcga tgtgcgccga cgcgcacgat ccggtcggcg tgtacttcgg cacgaccagc
     4321 ggcgaagtct gggcgagcgc gaacgagggc gaccgctgga catcgatcgc gcgccacctg
     4381 ccggaaattt actcggtcga agcggccggc ggctgaaatg aaggtcctca tcccctcgca
     4441 gcttcgcgac tacaccggcg gccgcgcgca cgtggaagcg cacggcgcga cgctcgctgc
     4501 gctcctcgcc gacctggagc ggacctaccc ggggatccgc ttccgcatga tcgacgagca
     4561 ggacgccatc cggcgccaca tcaagatctt cgtcaaccag cagaacacgc gcgacttgag
     4621 cgccgcgctc gccgcggacg acgaagtcgt tatcgtcggc gcgctgagcg gcggttaatc
     4681 ccggaaattc tggaactgca gcggaatatc ggtgacgctc ttccgcacca gcgcaatcgc
     4741 cgcctggaga tcgtcccgct tcgctccgga aacgcgcacc gccgtcccct ggatgctcgc
     4801 ctgcaccttc atcttcgagt ccttgaggag cttcacgatg ctcttcgcct tctcctgctc
     4861 gatgccggcc tt
//
LOCUS       DASPZD010000131         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1274565, whole genome shotgun sequence.
ACCESSION   DASPZD010000131 DASPZD010000000
VERSION     DASPZD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274565"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VEM33_02550"
     CDS             complement(<1..1256)
                     /locus_tag="VEM33_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYS50738.1"
                     /translation="MPPRKRPEELRSHRWYGVGDRKTFDHRSRTAQMGYDKSDYAGKP
                     VIAIINTWSDINPCHAHFRTRAEEVKRGVWQAGGFPIEMPAIALAEPFQKPSTMMYRN
                     FLAMETEELLRSYPADGCVLMGGCDKTTPALVMGALSMGLPMIYLPAGPMLRGNWHGQ
                     VLGSGSDSRKYWAELRAGTITERDWEEIEEGIARSPGHCMTMGTASTMTSAAEALGLA
                     LPGSASIPAADSHHAKMATATGKRAVEMVWEDATPKNLLKPSSFDNAVTCVLACGGST
                     NAAIHLIAMARRAGVQLDLERFDAISRKTPVIANLRPSGKYLMEDFYYAGGLRALLAT
                     IGDLLDLDAKTVSGKTLGENLEGAKVFNADVILPREKALIPSGGLAVLRGNLAPDGAI
                     VKPAAAEPRLLQHKGRALVFEDYDDL"
     gene            1389..2357
                     /locus_tag="VEM33_02555"
     CDS             1389..2357
                     /locus_tag="VEM33_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50739.1"
                     /translation="MQVTWAKRKGFVFAALAALALPVQAQEWPKQRPVQFIVAFGPGS
                     TTDIVARLVAQKLGESLGQSVVVENKPGAGGNIGAQFVKRAAPDGYTVLVISVAYAVN
                     PSLYASAGYDPLKDFVPVILGPSTPNIITVHPSVQANNLQELIALARKEKLAYASSGI
                     GTTTHLSMERIKIATGADITHVPYQPAQAVTAAVAGQTQISSTSMPPAVPQIKAGKLR
                     PIAVTSAQRSAALPDVPTVNEQGFKDFDDLTWIGFFLPAGAGPDVVNRLNGEINRIVE
                     LPDVRDRLSQNGLTSKRNSPAEFAAFIREEIPKWAKAVKDSGAKAD"
     gene            2366..>2831
                     /locus_tag="VEM33_02560"
     CDS             2366..>2831
                     /locus_tag="VEM33_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HYS50740.1"
                     /translation="MIDKIVPSVEAALADVSDGSTILVGGFGGAGVPEELIDALIAQG
                     AKDLTIVNNNAGNAEFGVAALLKAGRVRRIVCSYPRTADSYVFDALYRAGKIELELVP
                     QGTLSERMRAAGAGIGAFYTPTGYGTDLAKGKETRTIEGKGCVLEHPLCGDVA"
BASE COUNT          479 a          939 c          933 g          480 t
ORIGIN      
        1 tggaggtcgt cgtagtcctc gaacacgagc gcgcggccct tgtgctgcag caggcgcggc
       61 tccgcggcgg ccggcttgac gatggcgccg tcgggcgcga ggttgccgcg caggaccgcg
      121 aggccgcccg aggggatcag cgccttctcg cgcggaagga tcacgtcggc attgaacacc
      181 tttgcgcctt cgaggttctc gccgagcgtc ttgccgctca ccgtcttcgc gtcgagatcg
      241 agcaggtcgc cgatcgtggc gagcagcgcg cgcaagcctc ccgcgtagta gaaatcctcc
      301 atcaggtatt tccccgaagg gcgcaggttg gcgatcacgg gagtcttgcg cgagatcgcg
      361 tcgaagcgct cgaggtcgag ctgcactccc gccctccgcg ccatcgcgat gagatggatc
      421 gcggcgttgg tcgagccgcc gcaggcgagc acgcaagtca ccgcgttgtc gaacgaggag
      481 ggcttgagga gatttttcgg agtggcgtcc tcccacacca tctcgaccgc gcgctttccg
      541 gtcgcggtcg ccatcttcgc gtggtgggag tcggcagcgg ggatagacgc cgacccggga
      601 agcgcgaggc cgagcgcttc cgcagccgag gtcatggtcg atgcggtgcc catcgtcatg
      661 cagtggccgg gggagcgcgc gatgccttcc tcgatctctt cccagtccct ctccgtgatc
      721 gttccggcgc gcagctccgc ccagtacttg cgcgagtcgc tgcccgagcc gagcacctgc
      781 ccgtgccagt tgccgcgcag catcggcccc gcgggcaggt agatcatcgg cagtcccatc
      841 gacagcgcac ccatgacgag agcgggcgtc gtcttgtcgc agccgcccat cagcacgcag
      901 ccgtcggcgg gatacgagcg caacaactcc tcggtttcca tcgcgaggaa gttgcggtac
      961 atcatcgtgg agggcttctg gaacggctcg gcgagcgcga tcgccggcat ctcgatcggg
     1021 aatccgcccg cctgccacac gccgcgcttg acttcctcgg cgcgcgtgcg gaaatgcgcg
     1081 tggcaggggt tgatgtcgct ccaggtgttg atgatggcga tcacgggttt tcccgcgtaa
     1141 tcgctcttgt cgtagcccat ctgcgcggtg cgcgagcggt gatcgaaggt cttgcggtcg
     1201 cccacgccgt accagcggtg gctgcgcagc tcttcggggc gttttctcgg gggcattccg
     1261 gcggtcttgg tttcgagctg caattctacc cggacaattc gcgaagcgcg tgctgctttt
     1321 cacaggcaaa cgcgatatcc tctttgctct cgcggagcga atcggccgcg gaccgaggag
     1381 gaccagaaat gcaggttacg tgggccaaga ggaagggttt cgtttttgcg gcgctcgccg
     1441 cgctcgctct tccggtccag gcgcaagagt ggccgaagca gcgtcccgtg cagttcatcg
     1501 tcgccttcgg cccgggaagc accaccgaca tcgtcgctcg cctggtcgcg cagaagctcg
     1561 gcgaatcgct gggacaatcc gtcgtggtgg agaacaagcc cggtgcgggc ggcaatatcg
     1621 gcgcccagtt cgtcaagcgc gccgcgcccg acggctacac ggtgctcgtg atcagcgtcg
     1681 cttacgccgt caaccccagc ctgtacgcca gcgcgggcta cgatcccttg aaggatttcg
     1741 tcccggtgat cctcggtccg agcacaccca acatcatcac ggtgcatcct tcggtacagg
     1801 cgaacaacct ccaggaactg atcgcgctcg cgcggaagga gaaactcgcc tacgcatcct
     1861 cgggcatcgg cacgacgacg catctttcga tggagcgcat caagatcgcg accggtgcgg
     1921 acatcaccca cgttccctat cagccggctc aggccgtgac cgcagcggtt gcgggacaga
     1981 cgcagatttc gagcacctcc atgccgcccg cggtgccgca gatcaaagcg gggaagctcc
     2041 gtcccatcgc ggtgacgagc gcgcagcgct ctgcggcact ccccgacgtg ccgacggtga
     2101 acgagcaggg cttcaaggac ttcgacgacc tgacctggat cggattcttc ctgcctgcgg
     2161 gcgcgggccc cgacgtcgtc aaccgcctga acggcgagat caatcgcatc gtcgagctgc
     2221 ccgacgtgag ggacaggctt tcgcaaaacg gcctgacctc caagcgcaac agcccggccg
     2281 agttcgccgc cttcatccgc gaagaaatcc ccaaatgggc gaaagcggtc aaggactcgg
     2341 gcgcgaaggc cgattgatcg ccggcatgat cgacaagatc gttccctctg tcgaagccgc
     2401 gctcgccgac gtgagcgacg gctcgacgat cctcgtcggc gggttcggcg gcgcaggcgt
     2461 ccccgaggag ctgatcgacg cgctgatcgc acaaggggcg aaagacctga ccatcgtcaa
     2521 caacaacgcc ggcaacgcag aattcggcgt cgcggcgctg ctgaaagccg gtcgcgtgcg
     2581 caggatcgtc tgctcgtatc cgcgcacggc cgattcctac gtgttcgatg cgctctaccg
     2641 cgccgggaaa atcgagctcg agctggtgcc gcaggggacg ctctcggagc gcatgcgcgc
     2701 cgcaggcgcc gggatcggcg ccttctacac gcccacaggc tacggcaccg atctcgccaa
     2761 gggcaaggag acgcggacga tcgagggcaa aggatgcgtg ctcgagcatc cgctttgcgg
     2821 cgacgtggcc c
//
LOCUS       DASPZD010000132         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_326343, whole genome shotgun sequence.
ACCESSION   DASPZD010000132 DASPZD010000000
VERSION     DASPZD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326343"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEM33_02565"
     CDS             <1..261
                     /locus_tag="VEM33_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HYS50741.1"
                     /translation="PLTGLDAEVVAFLEREPKVGTMLGDIRRFVETWLPAYGQDSRSS
                     LTVAVGCTGGRHRSVYLVERLGAYFRSHTPVLVRHRHLAAAA"
     gene            258..866
                     /locus_tag="VEM33_02570"
     CDS             258..866
                     /locus_tag="VEM33_02570"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF004685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HYS50742.1"
                     /translation="MNPTVAVALTGASGMPYGLRLVETLIRAEARVYLLYSQAAHVVA
                     KQELDIALPPRAADAERMFGERYSAKPGQLRVFGREDWFAPVASGSNPPDAMVICPCT
                     VGTLAAVAAGLSDNLIERAADVTLKEGRKLILVPRETPFSALHLENMLKLARAGAVIL
                     PANPGFYHRPKRVEDLVDFIVARILDHLGIKHDLMRRWGEER"
     gene            880..1473
                     /locus_tag="VEM33_02575"
     CDS             880..1473
                     /locus_tag="VEM33_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYS50743.1"
                     /translation="MESLRVPIFPLNTVLFPGGLLPLRVFEARYMDMTRECLKRNEPF
                     GVCLIHQGSEVGAPAVPEGVGCLAKIQECDMQQQGILNLKTRGSQRFRILERETGTQG
                     LISAGVELIEPDASVAVPEKFSACTRLLQMVVADQGKSVFAEPHAFDDAAWVGYRLTE
                     ILPVPLVGKQKLLELTDSLARLSILQRFLESRGLAAA"
     gene            complement(1523..2608)
                     /gene="mutY"
                     /locus_tag="VEM33_02580"
     CDS             complement(1523..2608)
                     /gene="mutY"
                     /locus_tag="VEM33_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYS50744.1"
                     /translation="MSALASRLIRWQVRHGRHDLPWQRTRDPYRIWLSEVMLQQTQVA
                     TVIPYYERFLARFPDVKSLAGAPLDDVLALWSGLGYYSRARNLHAAAQVLVESWGGRF
                     PRTREALASLPGLGRSTAAAIAVFAFGEREAILDGNVKRVLARHFAVRGFPGERRVGN
                     RLWKLAESQVPGKDVERYTQALMDLGAGVCTRTRPACASCPLRASCEARSEGKAEAYP
                     RPRPRNPVPLRKTAMLLLLRGGEVLLEKRPPAGLWGGLWCLPEISPRADPRDYCRRRL
                     GARLASATRLPLLRHGFTHFTLDITPVVCRVASASPCASEPGQVWLPVEEAAQAAVPA
                     PVRKLLNSLWKTGDRPRFSTAAYGPDR"
     gene            complement(2605..>2801)
                     /locus_tag="VEM33_02585"
     CDS             complement(2605..>2801)
                     /locus_tag="VEM33_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50745.1"
                     /translation="SSGQGGKDLAELRGVTVPVKVSGPLDAPRFRADLRAAVGDAVKQ
                     RAEEKLKERMQDRLKDLLRR"
BASE COUNT          458 a          986 c          893 g          464 t
ORIGIN      
        1 cccctgaccg gtctggacgc ggaggtcgtc gcgtttctcg agagggagcc gaaggtcggc
       61 acgatgctgg gggatatccg aagattcgtc gagacctggc tgcccgcata cgggcaggac
      121 agccgcagct cgctcacggt cgccgtcggc tgcaccggcg gacggcaccg ctcggtatac
      181 ctcgtcgagc gcctcggcgc ttattttcgc agccatacgc cggtgctggt tcgtcatcgg
      241 cacctcgccg ctgcagcatg aacccgactg tcgccgtcgc gctgaccggc gcttccggaa
      301 tgccctacgg cctgaggctg gtggaaaccc tgatacgcgc cgaagcccgg gtctacctcc
      361 tctattccca ggccgcccac gtggtcgcga agcaggagct ggacatcgca ctgccgccgc
      421 gcgccgccga cgccgagcgc atgttcggcg agcgctactc ggccaagccc ggccagttgc
      481 gcgtgttcgg ccgcgaggac tggtttgccc cggtcgcttc cggttcgaat ccccccgacg
      541 ccatggtcat ctgtccctgc accgtgggca ctctcgcagc ggtcgccgcg ggtctctccg
      601 acaatctcat cgagcgcgcg gccgacgtga cattgaagga aggccgcaag ctgattctcg
      661 tcccgcgcga gacgccgttt tcggcgctgc acctggagaa catgctcaag ctcgcgcgcg
      721 cgggcgcggt catcctcccc gcgaatcccg ggttttacca ccgccccaag cgcgtcgagg
      781 acctggtcga tttcatcgtc gcgcgaatac tcgatcacct cgggatcaag cacgatttga
      841 tgcgccgctg gggcgaagag cgctaggctg gactcgctct tggaatcgct gcgcgtcccg
      901 atttttccgc tcaacacggt gctcttcccg ggaggcttgc tcccgctgcg ggtattcgaa
      961 gcgcgctaca tggacatgac gcgcgaatgc ctcaagcgca acgaaccgtt cggagtgtgc
     1021 ctcatccatc aagggtcgga ggtcggcgcg cccgccgtgc ctgaaggggt cgggtgtctt
     1081 gcgaagatcc aggaatgcga catgcagcag cagggcatcc tgaacctcaa gacccgcggc
     1141 agccagcggt ttcggatcct cgaacgtgaa accggcaccc agggtctgat ctccgccggc
     1201 gtagaactga tagagcccga cgcgagcgtc gccgttcccg agaagttttc ggcctgcacg
     1261 cggcttctgc agatggtcgt cgccgaccag ggcaagtccg ttttcgccga gccgcacgcc
     1321 ttcgacgacg ccgcgtgggt cggctaccgc ctgaccgaga tccttccggt gcccctggtc
     1381 ggcaagcaga agctcctcga gctgaccgac agcctggcgc ggctctccat cctgcagaga
     1441 tttctcgaaa gccgcggtct cgccgccgcc tgaaaccggg cgccgcctga aaccggggac
     1501 agaccccggt tttccgtcag cgtcagcggt ccggtccgta agccgctgtg gaaaaccggg
     1561 gtctgtcccc ggttttccac agagaattga gcagttttct cacgggcgcc ggtaccgccg
     1621 cctgggccgc ttcctctacg ggaagccaga cttgtccggg ctccgaggcg catggcgagg
     1681 cgctcgcaac gcggcacacg accggcgtga tatccagcgt gaaatgggtg aaaccgtgcc
     1741 gcaaaagcgg caggcgcgta gcgctcgcga gccttgcgcc caatcggcgc ctgcagtaat
     1801 cgcgcggatc ggcgcgcggg gaaatctccg ggagacacca taggccgccc cacaatccgg
     1861 ccggcggacg cttctccagc agtacttcgc ctccgcgcag aagcagcagc atcgcggttt
     1921 tcctgagcgg cacgggattg cgcggacgcg gccgcggata cgcttccgcc tttccttccg
     1981 aacgggcctc gcagctcgcc ctcaagggac agctcgcgca cgccggccgc gttcgagtac
     2041 aaacacccgc gcccagatcc atcaacgcct gggtgtagcg ctcgacgtcc ttcccgggca
     2101 cctgcgactc ggcgagcttc cacaggcggt tcccgacccg ccgctctccc gggaagccgc
     2161 gcaccgcgaa atgacgcgca agcacgcgtt tcacgttgcc gtcgaggatt gcttcgcgtt
     2221 cgccgaaggc gaataccgcg atcgcggccg cggtggaacg gcccaacccc ggcagcgatg
     2281 cgagcgcctc gcgcgtccgc gggaaccggc cgccccacga ttcgaccagg acttgcgccg
     2341 cggcatgcag gttgcgcgcc cgcgagtagt agcccaggcc gctccacagc gcgagcacgt
     2401 cgtcgagagg cgcccccgcg agcgatttca cgtccggaaa gcgcgcgagg aagcgctcgt
     2461 agtacggaat gaccgtcgcg acctgggtct gctgcagcat cacttccgag agccagatgc
     2521 gatacggatc gcgcgtccgc tgccacggca ggtcatgacg cccgtgccgg acttgccagc
     2581 gtataagccg gctcgctagg gcgctcaccg tctgagaaga tccttcagcc ggtcctgcat
     2641 gcgctccttc agcttctcct cggccctttg cttgaccgca tcgccgacag cggcgcggag
     2701 atcggcacgg aagcgcggcg catcgagcgg gccgctgacc ttcaccggca cggtgacgcc
     2761 acgcagttcg gcgagatcct tgccgccctg accgctcgag g
//
LOCUS       DASPZD010000133         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_64330, whole genome shotgun sequence.
ACCESSION   DASPZD010000133 DASPZD010000000
VERSION     DASPZD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64330"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VEM33_02590"
     CDS             complement(<1..335)
                     /locus_tag="VEM33_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYS50746.1"
                     /translation="MRRPAAARMKGDRTTLRIEERPGEVLLATLDRPEVANAFDTGAA
                     LELCELFERFRTEQRYRCVVITGAGDRAFCAGADLKERESLSDEQWRSQHEVFERMFR
                     AVRDCPVPA"
     gene            complement(354..959)
                     /locus_tag="VEM33_02595"
     CDS             complement(354..959)
                     /locus_tag="VEM33_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50747.1"
                     /translation="MKKFALLLSAGALALSGTVAQAQTRMFVRGTIASLDGDVLSVKS
                     RDGKDLKINLAPKFGVRYAKALTLADIKPGDFVGPASRKRADGSLEAISLQVFTPDRR
                     GQVPEGHTPWDLEPGSLMTNAIIGGMVQTSKGRELTLEYKGGSQKVFIPEGIPIFTAL
                     PGDRSMLVPGAYVVIGAQVAPDGTLSAAGGTVSKDGVKPAN"
     gene            complement(1088..2083)
                     /locus_tag="VEM33_02600"
     CDS             complement(1088..2083)
                     /locus_tag="VEM33_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50748.1"
                     /translation="MSHHAPIDVHAHYFPQTFLDLVAKHGPSHGFEYRMVEGKGPQFK
                     HGHLMTGPVGPKFIDLDARLAAMGEQGVEVHALSLSQPMVYWADGDLARRISEIYNDA
                     LAQAHQRHPKRLVGLATLPMHEPALAVKEVERAAKLPGMRGFYIATQIRGKDLSDASF
                     LPVYERIETSGLPLFLHPVEVIGHQRLAAYYLTNLLGNPFESAVAAAHLIFGGVLDRF
                     PKLVVCLPHAGGAFPWLVGRLNRGWEKRADLKHVKHAPLEYLRRFYYDTVGYSDHVLE
                     YLVKVIGPDRVLMGSDYCFPIAYEQPVKIVADHPALDAEAKRKIVETNARHLLKL"
     gene            2141..3037
                     /locus_tag="VEM33_02605"
     CDS             2141..3037
                     /locus_tag="VEM33_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYS50749.1"
                     /translation="MTTAPEEIEGMEERVGFESDGLKLAGILHLPDAGPAERRAAFLV
                     LHGFGSNKDGGGGTTVAKMLAGLGYAALRFDFRGCGESEGKRGRVICKEQVKDTRNAL
                     SFLATRPEIEPRRVGVVGQSFGAAVAVYAAGVDRRVAACISSGGWGDGAKKFRKQHAS
                     PRAWKKFSAMMQEGRRRKRAGKPMMVPRYDIVPIPPRLRGNLAPGSIMEFPFEVVETM
                     YAFNANEVVSRIAPRPLLLLHSANDSVTPTEQSVELFERAGRPTDLHLIADVDHFMFS
                     EGNTLVVNLVRNWLAKYFPARV"
BASE COUNT          538 a          972 c          993 g          544 t
ORIGIN      
        1 atcgccggga ccgggcaatc gcggaccgca cgaaacatgc gctcgaacac ctcgtgctgc
       61 gagcgccact gttcgtcgct gagcgattcg cgctccttca gatcggcgcc ggcgcaaaag
      121 gccctgtcgc cggcgccggt gatgacgacg catcggtacc gctgttcggt ccggaagcgc
      181 tcgaatagtt cacacagttc gagcgccgct ccggtgtcga acgcattggc gacctcgggg
      241 cgatcgagcg tcgccagcaa tacttcgccg ggccgttctt cgatccgcag cgtcgtgcga
      301 tcccccttca tgcgtgccgc ggccggccgc cgcaccgaac gggacgtacg cccctaattc
      361 gccggtttga caccgtcctt gctgaccgtg ccgcccgccg ccgacaaggt tccgtcaggc
      421 gcgacctggg cgccgatgac gacataggca cccggcacca gcatcgagcg gtcgcccggc
      481 agcgcggtaa aaatgggaat gccctcgggg atgaagactt tttgcgagcc gcccttgtat
      541 tcgagagtca gctcgcgccc tttcgaagtc tggaccatgc ccccgatgat agcgttggtc
      601 atgagcgagc ccggctcgag gtcccaaggc gtgtgccctt caggcacctg gccgcggcgg
      661 tccggggtga acacctggag ggatatcgct tcgagcgagc cgtcggcacg ctttctcgat
      721 gccgggccga cgaagtcgcc cggcttgatg tccgccaatg tgagcgcttt ggcgtagcgc
      781 actccgaact tgggtgcgag attgatcttc aggtccttac cgtcgcgcga cttcactgac
      841 aggacgtccc cgtccagcga ggcgatggtg ccgcgcacga acatgcgcgt ttgcgcctgg
      901 gcaacggtgc ccgagagcgc aagagcgccg gctgacagca agagtgcgaa tttcttcact
      961 gtgattcctc cttgagtgat ccgtccgggc caacccggca ggccgatagt aaacgcgatc
     1021 caacgcactt ccattccgga gcgctgcaac tattctgaca gattccgcct caaccctggc
     1081 ggggccccta gagcttgagc aggtggcgcg cgttcgtctc gacgatcttt cgcttggcct
     1141 cggcatcgag ggcagggtga tcggcgacaa tcttcaccgg ttgctcgtag gctatcggaa
     1201 agcagtagtc gctccccatc aggactcggt cggggccgat caccttgacc agatattcga
     1261 gcacgtgatc ggagtagccg accgtgtcgt agtagaagcg gcgcaggtac tcgagcggcg
     1321 cgtgcttgac gtgcttcagg tcggcgcgct tttcccaccc ccggttgagc cggcccacga
     1381 gccacggaaa agcgccgcca gcgtgtggaa ggcagacgac gagcttggga aaccgatcga
     1441 gcacgccccc gaagatgagg tgcgccgcgg cgaccgccga ctcgaaaggg ttgccgagca
     1501 gattggtgag gtagtaagca gcaagacgct ggtgcccgat cacctcgacc ggatgcagga
     1561 aaagcggcag gccgctcgtc tcgatgcgtt catagaccgg caggaatgaa gcgtcggaca
     1621 agtcctttcc gcgaatctgg gtggcgatgt agaagccgcg catgcccgga agtttcgcgg
     1681 cgcgctccac ttccttcacc gccagcgcgg gctcgtgcat cggcagcgtc gcgagtccca
     1741 cgagccgctt cggatgccgc tggtgcgcct gcgcgagcgc gtcgttgtag atctcgctga
     1801 tgcgtcgcgc gagatctccg tccgcccagt acaccatcgg ctgcgacaac gacagcgcgt
     1861 ggacctccac gccctgctcg cccatcgcag cgagccgggc gtccaggtcg atgaattttg
     1921 gcccgaccgg cccggtcatg agatggccgt gcttgaactg cgggcccttg ccctcgacca
     1981 ttctgtactc gaagccgtgg ctggggccgt gcttggcgac tagatcgagg aaggtttgcg
     2041 ggaagtagtg cgcgtgaacg tcgatcggtg cgtggtggct catcgcggct ccttttttcc
     2101 aacgattcta ccttggcgag aactgcccca tttataattc gtgacgacag ctccggagga
     2161 gatcgaagga atggaagagc gggtcggctt tgagtcggac gggttgaagc tcgcaggtat
     2221 ccttcacctg cccgatgcgg gaccggcgga gcggcgcgcc gcctttctcg tcctgcacgg
     2281 attcggcagc aacaaggacg gcggcggcgg cacgacggtc gcgaaaatgc tcgccggact
     2341 cggctacgcg gcgctgcgct tcgacttccg cggatgcgga gaaagcgaag gcaagcgcgg
     2401 gcgcgtcatc tgcaaggagc aggtcaaaga cacgaggaac gcgctgtcct tcctagccac
     2461 ccgtccggag atcgaaccaa ggcgcgtcgg cgtagtcggg cagagtttcg gcgccgcggt
     2521 cgccgtatac gcggcaggcg tcgaccggag ggtcgccgcc tgcatctcct cggggggctg
     2581 gggtgacggc gcgaagaagt tccgcaaaca gcacgcctcg ccccgcgcct ggaagaagtt
     2641 cagcgccatg atgcaggagg gaaggcgacg caagcgcgcc ggcaagccga tgatggtgcc
     2701 gcgctacgac atcgttccca ttccgccgcg cctccgcggc aacctcgcgc cgggctcgat
     2761 catggagttt ccgttcgagg tggtggaaac catgtacgcg ttcaacgcaa acgaggtcgt
     2821 cagcagaatc gcgcctcggc ccctgcttct gcttcattcg gcgaacgact cggtgacgcc
     2881 gaccgagcaa tcggtcgagc ttttcgagcg cgctggacgc ccgaccgatc tccacctcat
     2941 cgccgacgtc gaccatttca tgttctcgga ggggaacacg ctggtggtca atctcgtgcg
     3001 caattggctg gccaagtact ttcccgcgcg ggtctgatcc cggaatg
//
LOCUS       DASPZD010000134         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_787131, whole genome shotgun sequence.
ACCESSION   DASPZD010000134 DASPZD010000000
VERSION     DASPZD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787131"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VEM33_02610"
     CDS             complement(<1..1587)
                     /locus_tag="VEM33_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYS50750.1"
                     /translation="MKKPVSRILIINDEPLVLKEFVKGLNAAARSLDNPLGITFSGVT
                     TAREALAAIEEDGDLQAVVVDDKLYTLNQKSPKKEMSALELVQRVTRFRPELDVYILI
                     AQEHEDEIVDALFAEAVDGYFYREERDYRGMYRILNAQVQERSRTPFYDQLKNYVWMA
                     KDSWHTPGHSSGESLRGSPWVNDFCDFMGEHVFDADLSVSVKMLDSLMEPTGVIAEAQ
                     TIAAKAFGARRTFFATNGTSTSNKVIFQTLLAPGEKLLLDRNCHKSVHHGVVLSGGHP
                     IYLDSSINAKYSLYGPVPKKTILSAIRRHPDAQAIILTSCTYDGLRYDLAPIVEAAHA
                     KGIKVIVDEAWYGFARFHPAFRPTALEAGADYATQSTHKVLSAFSQASMIHVNDPGFN
                     EHLFRENFNMHTSTSPQYSMIASLDVARKQAVMEGYKLLSRTLALSQELRAQINSSGV
                     FRVLELEDLLPDEVRRDNILLDPTKVTVDISSCGYTVEDLQRELFDRYNIQVEKSTFN
                     TLTLLLTIGTTRSKVSRLYDA"
     gene            complement(1705..1971)
                     /gene="rpsT"
                     /locus_tag="VEM33_02615"
     CDS             complement(1705..1971)
                     /gene="rpsT"
                     /locus_tag="VEM33_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYS50751.1"
                     /translation="MANTKGARKAARQAEKRRKHNASLRSALTSAIKSVTTAIAAGDK
                     AAARKIFSKASGIMDRIADKNVVHKNEAARHKSRLAARLKAMPG"
     gene            2058..>3464
                     /gene="murJ"
                     /locus_tag="VEM33_02620"
     CDS             2058..>3464
                     /gene="murJ"
                     /locus_tag="VEM33_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYS50752.1"
                     /translation="MNLLKALATVSSMTFVSRILGFVRDALIARLFGAGLATDAFFVA
                     FRIPNLLRRLFAEGAFSQAFVPVLSEYRTRRGDSETKLLVDRTATLLTLALLAATALG
                     VAAATIVVYVSAPGFAATPAKFDLTVAMLRVTFPYILFISLVSLAGGVLNTWSRFAVP
                     ALTPTLLNLSFIFGALFLAPYFDPPVMVLAWSVFAGGVLQLAFQFPALAKIGMLPRPR
                     LSFSDPGVRRILGLMGPAAIGVSVAQISLLINTIIASWLGDGPISWLSYADRLMEFPN
                     GLFGVALGTVLLPSLSKYHAESSYDEYSKLLDWGLRLIFLLVTPAAVALALLSVPLVT
                     TLFHYGQFGEHDVWMTRQALVAYSIGLLGLILVKVLAPGFYARQNIATPVRIAVLTLI
                     ATQVMNAVFILSLKQGGLELATGIGPHAGLALAIGLAACLNAGLLYHKLRQHGIYHPQ
                     PGWRVFVLKIAAALAIMVL"
BASE COUNT          534 a         1101 c         1100 g          729 t
ORIGIN      
        1 cgcatcgtag aggcgcgaga ccttgctgcg cgtggtgccg atcgtcagca gcagggtcag
       61 cgtattgaac gtggatttct cgacctggat gttgtagcgg tcgaagagct cgcgctgcaa
      121 gtcctccacc gtatagccgc agctcgagat gtccacggtc accttggtcg ggtcgaggag
      181 gatgttgtct cgcctaacct cgtcgggcag aaggtcctcc aattcgagca cgcgaaagac
      241 gccactcgag ttgatctggg cgcgcagctc ctgcgagagc gcgagcgttc gcgaaagcag
      301 cttgtagcct tccatcaccg cctgcttccg cgccacgtcc aggctcgcga tcatgctgta
      361 ctgcgggctg gtggacgtgt gcatgttgaa gttctcccgg aacaggtgct cgttgaatcc
      421 gggatcgttc acgtggatca tcgaggcctg ggagaaagcc gagagcacct tgtgcgtgct
      481 ctgcgtggcg taatccgccc ccgcctcgag cgccgtgggc cggaacgccg gatggaagcg
      541 cgcgaacccg taccaggctt cgtccacgat caccttgatg cccttcgcgt gcgccgcctc
      601 gacgatgggc gcgaggtcgt agcgcagtcc gtcgtaggtg cacgaggtga ggatgatcgc
      661 ctgcgcgtcc gggtgcctgc ggatcgcgga gaggatggtc ttcttcggca ccgggccgta
      721 caagctgtac ttggcgttga tcgaggaatc gagatagatc ggatggccgc ccgagagcac
      781 cacgccgtgg tgcaccgact tgtggcagtt gcggtcgagc agcagctttt cgcccggcgc
      841 gagcagcgtc tgaaaaatga ccttgttcga ggtcgaggtc ccgttggttg cgaagaaggt
      901 gcggcgcgcg ccgaaggcct tggccgcgat ggtctgcgcc tcggcgatca cgccggtggg
      961 ctccatcagc gagtcgagca ttttcaccga caccgagaga tcggcatcga agacgtgttc
     1021 gcccatgaaa tcgcagaaat cgttgaccca ggggctgccg cgaagcgact cgcccgacga
     1081 atggcccggc gtgtgccagg agtccttcgc catccagacg tagttcttga gctggtcgta
     1141 gaacggcgtg cgcgaccgct cctggacctg cgcgttgagt atccggtaca tgccgcggta
     1201 gtcgcgctcc tcgcgataga agtagccgtc gaccgcctcc gcgaagaggg catcgacgat
     1261 ctcgtcctcg tgctcctgcg cgatcaggat gtagacatcg agctcgggcc ggaagcgcgt
     1321 gacgcgctgc acgagctcca gcgccgacat ctccttcttg gggctcttct ggttgagggt
     1381 gtagagcttg tcgtcgacca cgacggcctg gaggtcgccg tcctcctcga tcgcggcgag
     1441 cgcttcgcgc gccgtggtga cgccggaaaa ggtgatgccg agcggattgt cgagtgagcg
     1501 tgcggccgcg ttcagccctt tgacgaattc cttgagcacc aacggttcat cgttgatgat
     1561 gaggatccgg ctgaccggct ttttcatggg gtggcgcgca aaagagaaac cggattgttg
     1621 cgggaaaccg cgggtgagcg caaggaacgc caataaaaaa ggcggccgag ccgcctcttt
     1681 tccgccgcgt cgcctgactt gggactaccc cggcatcgcc ttgagtcgcg cggcaaggcg
     1741 gctcttgtgc cgcgccgcct cgttcttgtg gacgacgttc ttgtcggcga tgcgatccat
     1801 gattcccgag gctttgctga aaatcttgcg cgcggcggcc ttgtcgccgg cggcgatcgc
     1861 cgtggtgacg cttttgatcg cgctggtcag agcagagcgc aggctggcgt tgtgcttgcg
     1921 ccgcttctcg gcctggcgcg ccgccttgcg cgcgcctttt gtatttgcca tgcggaaccc
     1981 cgaacgaaag ggcgcaattg tagtcgtagt cgcaggcccg cgcaaggttt tccgccggtg
     2041 aaagctataa ttcccccatg aatttgctga aagcgcttgc gaccgtgagc agcatgacgt
     2101 tcgtgtcgcg cattctcgga ttcgtccggg acgctctgat cgcgaggttg ttcggcgctg
     2161 gcctcgcgac cgacgctttt ttcgtcgctt tccgaattcc caacctgctg cggcgcctgt
     2221 tcgccgaagg cgctttctcg caggccttcg tgcccgtgct ctcggaatac aggacccgtc
     2281 gaggcgactc ggaaaccaag ctgctcgtcg accgcaccgc gaccctcttg actctcgcgc
     2341 tactcgcggc cacggcgctc ggcgtcgccg ctgcgacgat cgtcgtctac gtgagcgcgc
     2401 ccgggttcgc ggcgacgccc gccaagttcg acctcaccgt ggcgatgctg cgcgtcacat
     2461 ttccctacat tctgttcatc tctctggtct cgctcgccgg aggcgtcctc aatacctgga
     2521 gccgtttcgc cgtaccggcg ctcactccga ctctgttgaa cctgtccttc atcttcggag
     2581 cgctgttcct cgcaccctat ttcgaccccc cggtgatggt gctcgcttgg tcggtgttcg
     2641 cgggcggcgt gcttcaactc gcgtttcagt tccccgcgct cgcgaaaatc ggcatgctgc
     2701 cgcggccgcg gctctcgttc tccgaccccg gagtgaggcg gattctcggg ctgatggggc
     2761 ccgcggcgat cggcgtatcg gtagcccaga tcagcctgct catcaacacc atcatcgcgt
     2821 cctggctcgg cgacggtccg atctcctggc tgagctatgc cgaccggctc atggagttcc
     2881 ccaacgggct gttcggcgtg gccctcggca cggtactgct tcccagcctc tccaagtatc
     2941 acgctgaatc gtcctacgac gagtattcga aattgctcga ctggggcttg cggctgatct
     3001 tcctcctggt gacgccggcc gcagttgcgc tcgccctctt gtccgttcct ttggtcacga
     3061 cgctgttcca ctacggccag ttcggcgagc acgatgtctg gatgacgcgc caggcgctgg
     3121 tcgcgtacag catcgggctg ctcggcctga ttctcgtcaa ggtgctggcg ccgggctttt
     3181 atgcgcggca gaatatcgct accccggtac ggatcgccgt cttgaccctg atcgccaccc
     3241 aggtgatgaa tgccgtgttc atcctgtctc tgaagcaggg aggactcgag cttgcgaccg
     3301 gcatcggtcc gcacgcgggc ctcgcgctcg cgatcggcct ggccgcttgc ctcaatgccg
     3361 ggctcctcta ccacaagctc aggcagcatg ggatctacca cccccaaccc ggctggcggg
     3421 tgttcgtatt gaagatcgcc gcggccctcg cgatcatggt tctg
//
LOCUS       DASPZD010000135         5580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1629740, whole genome shotgun sequence.
ACCESSION   DASPZD010000135 DASPZD010000000
VERSION     DASPZD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629740"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /gene="purH"
                     /locus_tag="VEM33_02625"
     CDS             complement(<1..1321)
                     /gene="purH"
                     /locus_tag="VEM33_02625"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYS50753.1"
                     /translation="MRTALISVSDKSGLAEFARGLARFKIKILSTGGTAKLLAEQGLV
                     ATEVADHTGFPEMLDGRVKTLHPRLHAGILARRDSPEHLAAIKAAGIEPIDLVVVNLY
                     PFAQTVAKPNCTLAEAIENIDIGGPSMLRAAAKNYAGVAVVTEPSDYSKVLAEMAERG
                     GALSEGTRFALARKAFAHSAAYEAAISNHLTGLGSDGELAPFPGRLTLQFEKVQDLRY
                     GENPHQAAAFYRDGNPTPGGIAGYTQIQGKELSYNNIADADAAWECVKTFDEPACVIV
                     KHANPCGAAIGASSLEAYELAFAADSAAAFGGIIAFNRKLDAATAEAVSKQFVEVVIA
                     PDFDAEARQLFAAKANVRVMSASLARAAQTYDFKRVGGGLLVQSPDARNVQASDLRIV
                     TRLKPTQAQIADLLFAWRVAKYVKSNAIVFCAGGRTLGIGAGQMSRVD"
     gene            complement(1333..1572)
                     /locus_tag="VEM33_02630"
     CDS             complement(1333..1572)
                     /locus_tag="VEM33_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYS50754.1"
                     /translation="MRQTNEIADCVRRSLERYFKDLDGAKPRAIYDMVLKNVERPMLE
                     LVLDRAEGNQSIAAEMLGINRNTLRKKIQSLKIKM"
     gene            complement(1569..2576)
                     /gene="dusB"
                     /locus_tag="VEM33_02635"
     CDS             complement(1569..2576)
                     /gene="dusB"
                     /locus_tag="VEM33_02635"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HYS50755.1"
                     /translation="MRIGQRTLRNNLFVAPMAGVTDRPFRQLCKKFGAGLAVSEMVAS
                     NSLLWGSEKTRRRANHEGEVEPISVQIAGADPVMMAEAARFNVERGAQIVDINMGCPA
                     KKVCNTLAGSALLKDEPLVGRILKAVVKAVSVPVTLKFRTGWDPVNKNALKVAKIAED
                     SGVQLLSLHGRTRACGFSGRAEYDTIREVKRSTRLPVVANGDITTPEEAKYVLEYTGA
                     DGIMIGRAAQGRPWIFREIEHYLSTGEKLPHPLVSEIHAVLLAHLHELYAFYGTETGV
                     KVARKHISWYTKGLAGSASFRHHMNQLETCEEQLNAVNRFLEQFGRENLRLRYDVELA
                     A"
     gene            complement(2722..3846)
                     /locus_tag="VEM33_02640"
     CDS             complement(2722..3846)
                     /locus_tag="VEM33_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYS50756.1"
                     /translation="MAELPEVDVAIAGGGPVGCALALALSGSALSVARIAGEADFADR
                     PIALSYGSRLILERLGAWSSVRSNPIETIHVSQQGFGRTVMRCADYGLPALGYVTAYS
                     ELVAQLAGRTPAGAESLKDWKSADDEVVLQLSRDEEKTGMRARLLVLADGGQNRRTPY
                     ARDYRQQAIVAEVTADHPRAGVAWERFTAEGPLALLPFKDRYALVWTVRPASAAELLG
                     LSDSDFLARLRETFGGRLGNFGSAGPRSTFPLSLRYGRIDPAPRVLAIGNAAQTLHPV
                     AGQGLNLGLRDAWELAQMLLDVAREEIGAPRFLARYARTRSFDRYAGIGFTDFLVRVF
                     SNSLAPLALARGAGLATLDALPPARSFLARRMIFGARALP"
     gene            complement(3839..5152)
                     /locus_tag="VEM33_02645"
     CDS             complement(3839..5152)
                     /locus_tag="VEM33_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HYS50757.1"
                     /translation="MDTTSNLSRCAERRHALQSRMGKGVAIIPTAAERIRNRDSDYLY
                     RFDSYFYYLSGFPEPEAVLVLVAGEEPRSILFCRERNPERELWEGVRNGPEGARAAFG
                     FDEAYPVGSLDEHMPKLLADQPALYYAPGADAAWDARIMGWLNQVRAQARAGVTAPAE
                     IRDVRTALDEMRLIKDDGELAVMRRAAAISVAAHERAMRATCPGRNEYEIEAELLYEF
                     RRRGAQYPAYWPIVAGGANACILHYRDNNARLADGDLLLIDAGCELDGYASDVTRTFP
                     VSGKFSGPQTDVYELVLAAQTAAIAAVKPGSRWDEPHDAAVRTLAQGLVDLGLCRGTL
                     EKVIETEDYKRFYMHRTGHWLGLDVHDAGEYKSGGQWRHLEPGMTLTVEPGCYIRPAD
                     DVPRQFWNTGVRIEDDVVVTSSGCEVLTAAAPKSVRDLEALVGRG"
     gene            complement(5185..>5580)
                     /locus_tag="VEM33_02650"
     CDS             complement(5185..>5580)
                     /locus_tag="VEM33_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HYS50758.1"
                     /translation="QALPLLGDEPFLVVNGDLFCDFDFSGLVGTDLGQDLAHLVLVPN
                     PPHHPQGDFALNGTQVTEAGPSTLTFSGIGLYRPELFASIRPGSKGQIATLLRAAMAR
                     GRVSGELHRGIWHDVGTPTRLSALNERVA"
BASE COUNT          981 a         1777 c         1789 g         1033 t
ORIGIN      
        1 cgtcgacgcg gctcatttgg cctgcgccga taccgagcgt ccggccgccg gcacagaaca
       61 cgatggcgtt cgacttgacg tacttcgcca cgcgccaggc gaacagcaaa tcggcgattt
      121 gcgcctgagt aggtttgagt ctggtgacga tgcgaaggtc cgacgcctgc acgttgcgcg
      181 cgtcgggaga ttgcaccagc aaaccgccgc cgacgcgttt gaagtcgtat gtttgtgctg
      241 cgcgggcaag gctcgctgac atcacccgta cgttggcttt ggcggcgaag agctgtcggg
      301 cttcggcgtc gaagtcgggc gcgatcacca cctcgacaaa ctgtttggat accgcctcgg
      361 cagtcgcggc atcgagcttg cggttgaagg cgatgatgcc tccgaacgca gcagcagagt
      421 cggcggcgaa agcgagctca tacgcctcca gggatgaagc tccgatcgcg gcaccgcagg
      481 gattggcgtg cttgacgatc acgcacgccg gctcatcgaa ggtcttcacg cattcccagg
      541 ctgcgtcggc gtcggcgatg ttgttgtacg acagctcctt gccttgaatc tgagtgtagc
      601 cggcgatgcc gccgggagtc ggattgccgt cgcgatagaa tgccgcagcc tgatgcgggt
      661 tttcgccgta gcgcaggtcc tgcacttttt cgaactggag cgtaaggcgc ccggggaaag
      721 gcgcgagttc cccatcactc cccagccccg tgaggtgatt gctgatggcg gcttcgtaag
      781 ccgcgctgtg ggcaaacgcc tttcgggcca gagcgaagcg cgtgccctcg ctcaaggccc
      841 cgccgcgctc cgccatttcc gcgaggacct ttgaatagtc ggacggttcg gtcacgactg
      901 caacgcccgc gtagttcttg gccgctgcgc gcagcatgct gggacccccg atgtcgatgt
      961 tctcgatcgc ctcggcgaga gtgcaattcg gtttcgcgac ggtttgtgcg aacggataga
     1021 ggttgaccac gacgagatcg atcggctcga tgcccgccgc cttgatcgcg gccaagtgct
     1081 cgggggaatc gcgccgggcg agtatgccgg cgtgcagtct tgggtgcagg gttttcacgc
     1141 gcccgtcgag catttccggg aagcccgtat ggtcggccac ttcggttgcg acgaggcctt
     1201 gctcggcgag aagtttcgcc gtgccgcctg tcgacaggat tttgatcttg aaccgggcga
     1261 ggccgcgcgc aaactccgcg aggccgctct tatcggatac gctgatcagc gccgtccgga
     1321 tgcaaatacc gttcacatct ttattttgag cgactggatc ttctttcgca gtgtattgcg
     1381 gttgatcccg agcatctcgg cggcgatgga ctgatttccc tcggcccggt cgagcacgag
     1441 ctcgagcatc ggacgctcga cgttcttgag caccatgtcg tagatggcgc gcggctttgc
     1501 cccgtcgagg tctttgaagt accgctccag agaccggcgg acacagtccg cgatctcgtt
     1561 tgtctgcctc atgccgccag ctccacgtca tagcgcaggc gcagattctc gcgcccgaac
     1621 tgctcgagga aacgattgac cgcgttgagt tgctcttcgc aggtttcaag ctgattcatg
     1681 tgatgccgga agctcgccga gccggcgagc cctttcgtat accaggagat gtgctttcgc
     1741 gcgaccttga ctcccgtttc cgttccatag aatgcgtaga gctcatgcag gtgcgcgagc
     1801 aacacggcat ggatctcgga aacgagggga tggggcaatt tctcgcccgt tgaaagatag
     1861 tgttcaattt cgcggaagat ccaggggcgc ccctgcgccg cgcggccgat catgatcccg
     1921 tcagcgcccg tatactcgag cacatacttc gcttcctcgg gcgtggttat atcgccgttt
     1981 gccaccacag gcaggcgcgt agaccgcttg acttcgcgga tcgtgtcgta ctcggcccgg
     2041 cccgagaagc cgcaagcgcg ggtgcgaccg tgcagggaca aaagctgcac gccgctgtcc
     2101 tcggcgatct tcgcgacctt cagagcgttc ttgttgaccg gatcccaccc cgttcggaac
     2161 ttcagcgtga caggtacgct caccgccttc accactgcct tgaggatgcg tccgacaagg
     2221 ggctcatcct tgagcagcgc ggatccggcc agggtgttgc agaccttttt cgccggacaa
     2281 cccatgttga tgtcgacgat ttgcgcgccc cgctccacgt tgaagcgggc ggcctccgcc
     2341 atcatgactg ggtcggcccc ggcgatctgc accgagatcg gctcgacctc cccttcgtga
     2401 ttggcgcggc ggcgagtttt ctcgctcccc cacagcaacg agttcgaggc caccatttcg
     2461 gatacggcca ggcccgcacc gaatttcttg cacagctgcc ggaaggggcg atccgtgacc
     2521 ccggccatcg gcgcgacaaa aagattattg cgcaacgtgc gctgaccgat tcgcatcgac
     2581 gatcaaggaa atttttctgg aggagcgcaa ttctatacct aaaaatgagc gcttagggaa
     2641 gcgctccacc gcttcgcgcg agaagaaaat cgaccgagga tttcctgttg tccccgcacc
     2701 gcaaaagcgg cgccgtgccg ttcagggcag cgcgcgagcg ccgaagatca tgcggcgggc
     2761 gagaaaactc ctcgcgggcg gcagggcatc gagcgttgca aggccggcac cgcgcgccag
     2821 cgcgagcggc gcaagagaat tcgagaacac tcgcaccaga aaatcggtga atccgattcc
     2881 ggcgtagcgg tcgaaactgc gagtgcgggc gtagcgagcg agaaagcggg gtgcgccaat
     2941 ttcttctctt gcgacgtcaa gcaacatctg agccagttcc catgcgtcgc gcaggccgag
     3001 attgagtccc tggccggcca ccgggtgcag cgtttgcgcg gcgttcccga tcgcgagaac
     3061 tctcggcgcg ggatcgatcc tgccataacg cagagagagc gggaaggtcg agcggggccc
     3121 ggccgagccg aaattcccga gccttccgcc gaaggtttcg cgcagccgcg cgaggaaatc
     3181 cgaatcggaa agcccgagca actcggcggc gctcgccggg cgcacggtcc agaccagtgc
     3241 gtagcggtct ttgaacggaa gcaaggcaag cggcccctcg gccgtgaagc gttcccaggc
     3301 gaccccggcg cgcgggtgat cggccgtgac ctcggccacg atggcttgct ggcgataatc
     3361 cctggcatac ggcgtgcggc gattctggcc gccgtcggcc agcacgagca gccgagcgcg
     3421 catgcctgtt ttctcttcgt ctcgggacag ctggagcaca acttcgtcgt cggccgattt
     3481 ccagtccttc aaggattccg cccctgcggg agtgcggccc gcaagctgcg cgacgagctc
     3541 ggaatacgcg gtgacgtagc ccagcgccgg caagccgtag tcagcgcagc gcattaccgt
     3601 tctgccgaaa ccctgctggc tgacatggat ggtttcgatc gggttgcttc ggaccgagct
     3661 ccatgcgccc aagcgctcca ggatgaggcg gctgccgtag gagagggcga taggtcgatc
     3721 tgcgaaatcg gcttcgcccg cgattcgggc gacggagagc gcgctcccgg acagggcaag
     3781 ggccaacgcg cacccgaccg gtccgccgcc ggcaatggcg acatccacct ccggcagctc
     3841 agccacggcc gaccagggcc tcgagatccc gaaccgattt cggtgcggcc gcagtcaata
     3901 cctcgcaccc cgaagacgtc acgacgacgt cgtcctcgat gcgcaccccc gtgttccaga
     3961 actggcgggg cacgtcatcg gccggtcgga tgtagcagcc gggctccacg gtcagggtca
     4021 tgcccggctc gaggtggcgc cattgcccgc ccgatttgta ttctcctgcg tcgtggacgt
     4081 ccagaccgag ccaatgtccg gtgcgatgca tgtagaagcg cttgtaatcc tcggtctcga
     4141 tcaccttttc gagcgtcccg cggcacagtc ccaaatccac gagtccttga gcgagcgtcc
     4201 ttaccgcggc atcgtgcggc tcgtcccagc ggctgcccgg cttcactgcc gcgatcgccg
     4261 cggtctgcgc tgcgagcacc agttcgtaga cgtccgtctg cggcccgctg aattttccgc
     4321 tcacggggaa agtccgcgtc acatccgagg cgtatccgtc cagctcgcag cctgcgtcga
     4381 tgagcaaaag gtctccgtcg gcgagccgcg cgttgttgtc gcggtaatgc aggatgcagg
     4441 cgttggctcc tccggcaacg atgggccagt acgccggata ctgggcgccg cggcggcgaa
     4501 actcgtagag aagctccgcc tcgatttcgt attcgttgcg tcctgggcac gtcgcgcgca
     4561 tcgcgcgttc gtgggcggcc accgagatcg ccgctgcgcg ccgcatcacg gcgagctccc
     4621 cgtcgtcctt gatgagacgc atctcgtcga gcgcggtgcg cacgtcgcgt atctcggcgg
     4681 gcgcggtgac gcccgcgcgc gcctgcgccc ggacttgatt cagccatccc atgatgcgcg
     4741 cgtcccaggc tgcatcggcc ccgggagcgt aataaagcgc aggctgatca gcgagcagct
     4801 tcggcatgtg ctcgtcgagc gaccccaccg gataggcttc gtcgaaacca aacgcggccc
     4861 tcgccccctc ggggccgttg cgaacgcctt cccagagctc gcgctccgga ttgcgctcgc
     4921 ggcagaagag aatggacctg ggttcctctc cggcgacgag gacgagcacc gcctcgggct
     4981 cggggaaccc gctcagatag tagaaatagc tgtcgaatcg ataaaggtag tcgctgtcgc
     5041 gattgcggat gcgttccgcc gcggtcggaa tgatcgcgac gcccttgccc atgcgcgact
     5101 gcagcgcgtg acggcgctcg gcacagcgcg aaaggttact agtggtgtcc attgccgcga
     5161 ttgtcgtacc ctttgcggcg acgatcaagc gacgcgctcg ttcagcgcgg aaagccgcgt
     5221 gggcgtgccg acgtcatgcc agatgccgcg atgcaactca ccggagaccc gcccgcgcgc
     5281 catcgccgca cgcaacagcg ttgcaatctg tccttttgag cccggtctga tgctcgcgaa
     5341 aagctccggg cggtaaagac ctatgccgct gaaagtgagc gtggatggtc ccgcttcggt
     5401 gacttgcgtg ccgttcagcg cgaagtcccc ttgcgggtga tgcggcggat tcgggaccag
     5461 taccaaatgc gcgagatctt gtcccaagtc ggtcccaacg agaccggaga agtcgaagtc
     5521 gcagaagagg tcgccgttga cgacgaggaa cggttcatcg ccgagtagag gaagcgcctg
//
LOCUS       DASPZD010000136         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1148102, whole genome shotgun sequence.
ACCESSION   DASPZD010000136 DASPZD010000000
VERSION     DASPZD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148102"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /gene="carA"
                     /locus_tag="VEM33_02655"
     CDS             <1..946
                     /gene="carA"
                     /locus_tag="VEM33_02655"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HYS50759.1"
                     /translation="TGANREDLESGKVFAAGLVVRDLSTAVSSWRAEGNLSDFLVSDK
                     VVAIAGIDTRRLTRILREKGAQNGAVLAGSDDADQALEAARAFPGLAGMDLAKVVTTA
                     KPYDWSEGRWQAGGGFGKGAAPRFHVVAYDYGVKRNILRMLVDRGCRVTVLPAQTPAT
                     DALALGPDGVFLSNGPGDPEPCDYAIRAIRQIIDAGIPTFGICLGHQLMGLASGARTV
                     KMKFGHHGANHPVKDLERGTVVITSQNHGFAVDPDTLPANLKATHVSLFDGSLQGLAR
                     TDRPAFCFQGHPEASPGPHDIGYLFDRFAKMMQERRSA"
     gene            939..>2585
                     /gene="carB"
                     /locus_tag="VEM33_02660"
     CDS             939..>2585
                     /gene="carB"
                     /locus_tag="VEM33_02660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYS50760.1"
                     /translation="MPKRTDIESVLIIGAGPIVIGQACEFDYSGAQACKALRAEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPVNWQMLEAVIARERPDALLPTMGGQTGLNCALDL
                     VRHGVLQKYGVEMIGASSEAIDKAEDREKFKQAMARIGLACPRSTIAHSLEEALQVQA
                     AIGFPAVIRPSFTLGGSGGGIAYNREEFVAICERGLEASPTRELLIEESVIGWKEFEM
                     EVVRDRKDNCIIVCSIENVDPMGVHTGDSITVAPAQTLTDKEYQIMRDAAIEVLREIG
                     VDTGGSNVQFAVNPKNGRMLVIEMNPRVSRSSALASKATGFPIAKVAAKLAIGYTLDE
                     LKNEITGGATPASFEPSIDYIVTKVPRFAFEKFPQANDRLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEVGVDGLNQKTTDREKLEKELGEPGPERIWYVGDAFESGFTLEEVHQL
                     TRIDPWFLAQIKEIVDLEMDLDDKRLDEIDADTLRGLKRRGFSDRRLAYLFNTGEAEV
                     RAVRHRFGIRPVYKRVDTCAAEFATRTAYMYSTYEEECEAS"
BASE COUNT          504 a          811 c          856 g          414 t
ORIGIN      
        1 taccggggcc aaccgcgagg acctggagtc gggaaaggtg ttcgccgcgg gtctggtggt
       61 cagggatctg tcaactgcgg tctcgagctg gcgggccgaa gggaatctct ccgattttct
      121 cgtctcggac aaagtggtcg ccatcgcggg catcgacacg agacgcctca ctcggatcct
      181 tcgggaaaaa ggcgcacaga acggcgccgt gctcgcgggt tccgatgacg cggatcaggc
      241 gctggaagcc gcgcgggcgt ttcccggtct tgcaggaatg gatttggcga aggtcgtgac
      301 caccgccaag ccctacgact ggtccgaagg gcggtggcag gccggcggcg ggttcggcaa
      361 gggagccgcc ccgcgctttc acgtcgtcgc gtacgactac ggcgtaaaac gcaacatact
      421 gcgcatgctc gtcgatcgcg gctgccgggt cacggttctt ccggcgcaga cgccggcaac
      481 ggatgcactt gcgctcgggc ccgacggggt gttcttgtcg aacggtccgg gcgacccgga
      541 accctgcgac tacgccatac gcgccatccg gcaaatcatc gacgccggga ttccgacctt
      601 cgggatctgc ctcggccacc agcttatggg actcgcgtcc ggagctcgga ccgtgaagat
      661 gaagttcggg catcacggtg ccaaccaccc ggtgaaggac ctggagagag gaaccgtcgt
      721 catcaccagc cagaatcacg ggttcgcagt cgacccggac actctcccgg cgaacctcaa
      781 ggccactcac gtatccctct tcgacggcag cctgcagggg ctcgcacgca ccgaccggcc
      841 ggcattctgc tttcaggggc accccgaggc gagccctggg ccgcacgata tcgggtatct
      901 cttcgaccgc ttcgccaaga tgatgcagga gcgccgcagt gcctaagcgc accgacatcg
      961 agagcgtgct catcatcggg gcagggccga tcgtcatcgg ccaggcctgc gagttcgatt
     1021 attccggcgc gcaggcctgc aaggcgctgc gcgccgaagg ctacaaggtg atcctggtga
     1081 attcgaatcc ggcaaccatc atgaccgacc cggagatggc cgatgtcacc tacatcgagc
     1141 cggtgaactg gcagatgctg gaggccgtca tcgcccggga gcggcccgat gcgctgctgc
     1201 cgaccatggg cggccagacc ggcctcaatt gcgcgctgga ccttgtccgg cacggcgtgc
     1261 tccagaagta cggggtcgag atgatcggcg cgagcagcga ggcgatagac aaggccgagg
     1321 accgcgaaaa attcaagcag gcgatggcga ggatagggct cgcctgcccg cgctcgacga
     1381 tcgcgcattc gctggaagag gccctgcagg ttcaggctgc gatcggtttt cccgccgtga
     1441 tccggccttc gttcactctc ggggggtcgg gcggcggcat cgcctacaac cgcgaggaat
     1501 tcgtggcgat ctgcgagcgc gggctggagg cgtcgcccac gcgcgagctg ctgatcgagg
     1561 aatcggtgat cggctggaag gaattcgaaa tggaggtggt gcgtgaccgc aaagacaact
     1621 gcatcatcgt ctgctcgatc gaaaacgtcg acccgatggg cgtgcatacg ggcgattcca
     1681 tcaccgtggc gcccgcgcag accttgacgg acaaggaata ccagatcatg cgcgacgcgg
     1741 cgatcgaggt cctgcgcgag atcggcgtcg acaccggcgg ctcgaacgtg caattcgccg
     1801 tcaatcccaa aaacggccgc atgctcgtga tcgagatgaa cccgcgcgtg tcgcgctcct
     1861 ccgccctggc gtccaaggcg accggcttcc cgatcgcgaa ggtcgcggcc aagctcgcga
     1921 tcggctacac cctcgacgag ctcaagaacg agatcaccgg cggcgcgact ccggcctcct
     1981 tcgaaccgag catcgactac atcgtcacca aggtcccgcg cttcgcgttc gagaaatttc
     2041 cacaggcgaa cgacaggctg acgacgcaga tgaaatccgt gggcgaggtc atggcgatcg
     2101 gccgcacttt ccaagagtcc ttccagaagg cgctgcgggg cctggaggtg ggcgtggatg
     2161 ggctcaatca gaagacgacc gatcgcgaga aactcgaaaa ggagcttggc gaaccggggc
     2221 cggagcgcat ctggtacgtg ggcgacgcct tcgagagcgg gttcacactc gaggaagtgc
     2281 accagctcac gcgcatcgac ccctggttcc tcgcgcagat caaggagatc gtcgacctgg
     2341 agatggacct cgacgacaaa cgcctcgacg agatcgacgc agatacgctg cgcggcctca
     2401 agcgcagggg cttctccgac aggaggctcg catacctgtt caacacgggc gaggcggagg
     2461 tacgtgcggt acgtcaccgc ttcggaatcc gcccggtgta caagcgcgtc gatacgtgcg
     2521 cggcggaatt cgcgacgcgg accgcgtaca tgtactcgac ctacgaagag gaatgcgagg
     2581 cttct
//
LOCUS       DASPZD010000137         4555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_303938, whole genome shotgun sequence.
ACCESSION   DASPZD010000137 DASPZD010000000
VERSION     DASPZD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303938"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VEM33_02665"
     CDS             complement(<1..716)
                     /locus_tag="VEM33_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYS50761.1"
                     /translation="MRFELKAVSPDGRVESLDLHGLDQASAIQQAESRGYTVLGVRAR
                     AGFVAPWRGGQARFPLVLFSQELLVLLEAGLPLVESIETLAERERRSEFRALLANVAS
                     ILRQGNSLSSALQQFPLAFPPLYVSTVQASEKTSDLAPALGRYVAYQNQLEAIRKRLI
                     NAAIYPALLIGVGGLVSLFLLLYVVPRFSRIYEERNVDLPIFSKILLSWGQLVEGHGA
                     LVMGILAGLVIAALYALRNAR"
     gene            complement(731..1153)
                     /gene="gspG"
                     /locus_tag="VEM33_02670"
     CDS             complement(731..1153)
                     /gene="gspG"
                     /locus_tag="VEM33_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HYS50762.1"
                     /translation="MNSAASSRTRGFTLLELLVVVVIIGLLVGYVAPRYFGQVGKSEV
                     TTAKAQIDALEKALDQYRLDTGRYPTTELGLAALVNRPPNEPKWNGPYLRKDAPLDPW
                     GKAYVYKQPGERSDYDILSYGKDGQPGGTGENADISNH"
     gene            complement(1342..2910)
                     /locus_tag="VEM33_02675"
     CDS             complement(1342..2910)
                     /locus_tag="VEM33_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYS50763.1"
                     /translation="MCTLAAACSQIPWDADPTADNERTFPVPVAAPAVSAPAAQPAST
                     AGVAPATTAPVAAHAAAPAQAPAATPAAAVPVPPVAKPQEAKEIEPPHAIDLARPADD
                     LWDRIRSGFAMADLESPLVGVRERWYASQPEYLKRMIERSKLYLYYIVEEVEKRGMPT
                     EIALLPMVESAFNPMAYSRSHASGLWQFIPSTGKTYKLSQNWWADSRRDVVASTNAAL
                     DYLQALYELHGDWHLALASYNYGENGVARAIERNRAKGLPTDYMSLKMPPETRGYVPK
                     LQALKNIISNPEAFGASFEPIPNEPYFVTVPTPSAIDIRLAATFAEMSVDELIALNPA
                     LNRPMISGPHTQTLVLPADRIDAFQRNLDAYDQPLTSWQPYTMKGGDNLERLAAKHGI
                     ALSKLKLANGITSRTRAGPGFQLLLPLKGSGVGAQPLPAVFRPPVRSAPRKGGLVHVV
                     KKGETLYGISRRYRVSTDSLLRWNHVGVLTTGQRLIIYRAPAKARKPVSPVKVSAAQP
                     KQNAVASLLPAGQQ"
     gene            complement(3099..3875)
                     /gene="gloB"
                     /locus_tag="VEM33_02680"
     CDS             complement(3099..3875)
                     /gene="gloB"
                     /locus_tag="VEM33_02680"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HYS50764.1"
                     /translation="MPDNGFQVIPLRAFKDNYVWTLRNAACAAVVDPGEARPVLDYLA
                     AEKLRLVAILATHHHPDHVGGIAEILRAHTVPVYGPRGEPITTLTHPVGGGDRVAVEQ
                     LGVAFEVLDIPGHTRAHIAYYGSNMVFCGDTLFACGCGRVFEGTPQQLYASLEKLAAL
                     PDDTLVYCGHEYTLANIGFAKAVEPSNSALLEREASDARLRRDNLPTLPSTIAREKAT
                     NPFLRVTRPAVIESANKYLGKRVSDPAGVFAALRQWKNQF"
     gene            3917..>4555
                     /locus_tag="VEM33_02685"
     CDS             3917..>4555
                     /locus_tag="VEM33_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYS50765.1"
                     /translation="MLADWFETPKGDYVLAWERGQFDAAVEDVFGYNAVQLGLPGLDF
                     LRESRIPLKVRAGLEPGCSLRCEPVQLPLAGQSIDLLALPHVLEFTAQPHAILRKAER
                     VLMPEGSIVISGFNPLSLWGAARALRRERQQVPWGGRFIGLLRLKDWLALLGFELTGG
                     RFGCYAPPFSQRRWLERVGFMEKAGERWWPICGGVYVVRAIKRNVGMRLVLPE"
BASE COUNT          757 a         1383 c         1582 g          833 t
ORIGIN      
        1 cgggcgttcc ggagcgcgta gagcgccgcg atcacgaggc cggcaaggat gcccatcacc
       61 agagccccgt ggccctccac cagctggccc catgagagca ggatcttcga aaagatcgga
      121 agatcgacgt tgcgctcctc gtagatccgg ctgaagcgcg gcaccacgta gagcagcagg
      181 aaaaggctca ccaagccccc tacgccgatg agaagcgccg gatagatggc cgcgttgatc
      241 aggcgcttgc ggatcgcttc gagctggttc tggtaggcga cgtagcgccc aagcgccgga
      301 gcgaggtcgc tcgtcttttc gctcgcctgc acggtggaga cgtaaagagg tggaaaggcg
      361 agcgggaact gctggagcgc cgaagagagg gaatttccct ggcgcaggat cgaggctacg
      421 ttcgcgagca aggcgcgaaa ttcgctgcgg cgctcgcgct ctgccagtgt ttcgatggat
      481 tcgaccaggg ggagtcccgc ctccagcagg acgagaagct cctggctgaa caggacgagg
      541 ggaaagcgcg cctgtccgcc gcgccagggc gcgacgaagc ccgcacgggc gcgcacgccg
      601 agcaccgtat agccgcggct ctcggcctgc tgaatcgcgc tcgcctggtc gagtccgtgc
      661 agatcgagcg actcgaccct tccatccggg ctgactgcct tgagctcgaa gcgcatcgtc
      721 cggtgccgcc ctagtggttg ctgatgtcgg cgttttcgcc ggttcccccc ggctggccgt
      781 ccttgccgta ggagaggatg tcgtagtcgc tgcgttcgcc gggttgcttg tacacgtagg
      841 ctttgcccca gggatcgagc ggcgcgtctt tgcgcaggta cgggccgttc cacttgggct
      901 cgttcggagg gcggttgacg agcgctgcga gtcccagctc cgtcgtcgga tagcggcccg
      961 tgtccaggcg atactggtca agcgcttttt cgagggcgtc gatctgggcc ttggccgtgg
     1021 tcacttcgga ctttccgact tggccgaaat agcgcggcgc gacgtatccg accagcagcc
     1081 cgatgatgac gacgaccacc agcagctcca gcagggtgaa gccgcgagtg cgcgaggaag
     1141 cagccgaatt catcgggtga tccatcgggg tcaggatcgc gtatagacgt tgcagcttac
     1201 tctaccaagt caatgttata tcgcaagccc ggctgcgcgg agccgcagac tgcggactgg
     1261 aacggggatt ttacatatga aaacagtggg gtgagcgcac gctggcgacg tgcggcgccc
     1321 ggaccggtct tgccaaccgg tctattgctg ccctgcggga agcagggatg cgacggcgtt
     1381 ctgcttcggc tgagcggccg aaaccttgac cggactgacg ggcttgcgcg ccttggccgg
     1441 cgcgcgatag atgatgagcc tctggccggt ggtgaggacg ccgacgtggt tccaacggag
     1501 caggctgtcg gtgctgacgc ggtaacgccg cgaaattccg tagagcgttt cgcctttctt
     1561 cacgacgtgc accaatccgc ctttgcgcgg cgcgctccgg accggaggac ggaacacggc
     1621 gggcaggggc tgcgcgccga cgcccgatcc cttgaggggc agcagcagct ggaagcccgg
     1681 gccggctctc gtgcgcgagg tgatgccgtt cgcgagcttg agcttggaga gcgcgatgcc
     1741 gtgtttcgcg gcgagccgct cgaggttgtc gccgcctttc atcgtgtacg gctgccagga
     1801 ggtgagcggc tggtcgtagg cgtcgaggtt tctctggaac gcgtcgatgc ggtccgcggg
     1861 cagcaccagg gtctgcgtgt gtgggcccga gatcatgggg cgattgaggg cggggttgag
     1921 cgcgatcagc tcgtccaccg acatctccgc gaacgtcgcc gcgagccgga tgtcgatggc
     1981 gctgggcgtg ggaacggtca cgaagtaggg ttcgttcggg atcggctcga agctcgcgcc
     2041 gaaagcctcc ggattcgaga tgatgttttt cagcgcctgg agcttgggca cgtagccgcg
     2101 cgtctcaggc ggcatcttga ggctcatgta atcggtcggc aggcccttcg cgcggtttcg
     2161 ttcgatcgcg cgcgccacac cgttttcacc gtagttatag gaggcgagcg ccagatgcca
     2221 gtcgccgtgc agctcgtaca aggcctgaag atagtcgagc gcggcattgg tggaggcgac
     2281 cacgtcgcgg cgcgagtccg cccaccagtt ctggctcagc ttgtaagtct tgccggtcga
     2341 aggaatgaat tgccacaggc cggaggcgtg cgagcgcgaa tacgccatgg gattgaacgc
     2401 gctctcgacc atgggcaaga gcgcaatctc ggtcggcatg ccgcgctttt cgacctcctc
     2461 gacgatgtag tagagataga gcttgctgcg ctcgatcatg cgcttcagat attcgggctg
     2521 gctcgcatac cagcgctcgc gaacgcccac gagcgggctc tccagatcgg ccatcgcgaa
     2581 accgctgcgg atgcggtccc acagatcgtc cgcggggcgg gcgaggtcga tcgcgtgagg
     2641 aggctcgatc tccttcgctt cctgcggctt ggcaaccggg ggaaccggga cggccgctgc
     2701 cggcgtggcg gccggggcct gagcgggtgc ggctgcatga gcagcgacag gggcggtggt
     2761 ggcgggggcg acgcccgcgg tcgacgcggg ctgcgctgca ggtgcggaaa ccgccggcgc
     2821 agcgaccggg accggaaacg tcctttcgtt gtcggccgtg ggatcggcgt cccaggggat
     2881 ttgcgagcac gcggcggcca aggtgcacat tgccgttgct gcgagcagcc tgcttaactt
     2941 ctcactcaag gcctggcctt ttcgtccgca aaagccgcga tggtacccat ggtttccata
     3001 cgggtcaagc aagtgggcga ttcagaagga ctttttacca gaaaatcgct ggcgcgacgc
     3061 ccgattaacg cgcgacggca cgattggtgt cagctcgatc agaattggtt cttccattgg
     3121 cgcagcgcgg cgaatacgcc ggcaggatcg ctgacgcgtt tgcccaggta cttgtttgcg
     3181 gattcgatga ccgcgggccg cgtcacgcgc agaaaagggt tggtggcctt ctcgcgggct
     3241 atcgtcgagg gaagcgtcgg gaggttgtcg cgccgcagac gcgcgtcact ggcctcgcgc
     3301 tcgagcagcg cggaattgct tggctcgacg gctttggcga agccgatgtt cgccagagtg
     3361 tactcgtgcc cgcaatacac gagcgtgtca tcggggagcg cagcaagctt ctcgagcgac
     3421 gcgtacagct gctgtggcgt tccctcgaat acgcgcccac atccgcaggc gaacagcgtg
     3481 tcgccacaga acaccatgtt tgaaccataa taagcaatgt gcgcgcgcgt gtgcccggga
     3541 atgtcgagca cctcgaacgc cacgcccagt tgttcgacgg cgaccctgtc tccgccgccg
     3601 accggatgcg taagcgtggt gatcggctcg ccgcggggtc cgtacaccgg aaccgtgtga
     3661 gctcgtagta tctccgcgat gcccccgaca tgatccgggt gatgatgggt agcgaggatc
     3721 gcgaccagcc gcagcttttc agccgcgaga tagtccagca cgggccgggc ttcacccgga
     3781 tcgaccaccg ctgcgcaagc cgcattgcgc agcgtccaga cgtagttgtc cttgaaagcc
     3841 cgcaatggga tgacttggaa tccgttatcc ggcatattcg tgagaggtaa gggtgctgca
     3901 agccgcgcaa aacgcaatgc tagccgattg gttcgagacc cccaagggcg actacgtgct
     3961 cgcctgggaa cgcgggcagt tcgacgccgc ggtcgaggac gtgttcggct acaacgccgt
     4021 gcagctcggc cttccgggtc ttgacttcct gcgcgagtcg cgcattccgc tcaaggtccg
     4081 cgccgggctc gagccgggct gcagcctgcg ctgcgagccg gtgcagttgc cgctcgccgg
     4141 gcaaagcatc gacctgctgg ctttgcctca cgtgctggag ttcactgctc aacctcacgc
     4201 gatcctgcgc aaggccgagc gggtgctgat gcccgagggc agcatcgtca tttccggatt
     4261 caatcccctg tcgctgtggg gtgcggcacg cgcgctccgc cgtgagcggc agcaggttcc
     4321 ctggggcggc aggttcatcg gtctgctgcg gctcaaggat tggctcgcgc tgctcggctt
     4381 cgagttgacc ggcggtcgct tcggctgcta cgccccgccg ttctcccagc gccgctggct
     4441 cgagcgcgtc ggcttcatgg agaaggcggg cgagcgctgg tggccgatct gcggcggagt
     4501 ttacgtcgtt cgcgcgatca aacgcaacgt gggcatgcgc ctggttctgc cggaa
//
LOCUS       DASPZD010000138         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_786503, whole genome shotgun sequence.
ACCESSION   DASPZD010000138 DASPZD010000000
VERSION     DASPZD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786503"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..562)
                     /gene="nudB"
                     /locus_tag="VEM33_02690"
     CDS             complement(101..562)
                     /gene="nudB"
                     /locus_tag="VEM33_02690"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HYS50766.1"
                     /translation="MTTVYKIPCSVLVVIHTPDLQVLMMERAGWKGFWQSVTGSLAHE
                     SESPRDAAIREVREETGLDAGRYDLRDWGIENRFEIFKKHRSRYAPGITHNLERVFSL
                     SLPEPLPVKLDPAEHLSYEWLPWREAAALTISWTNRDAILALPQRLGVSER"
     gene            849..1961
                     /locus_tag="VEM33_02695"
     CDS             849..1961
                     /locus_tag="VEM33_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYS50767.1"
                     /translation="MEMENVVTLTRRHPSRVNLRRESSVTAADLVMGLTVIIPAYNEA
                     GFVGDTIRSLLAQTVPPDQIILVDDGSTDGTGDVGRRAGTTVIRPPRNTGSKAGAQTF
                     ALPWVRTKLVMAIDADTTLEPTAVERLMRSFEDPGVGAACGFVLPRRIKTLWERGRFV
                     EYLFAFTFYKQVQEYYRRPMISSGCFSMYRTDLMRRHGGWPGRTLAEDMDLTWTFHQT
                     GVGVRFEPEAVCYPIEPYNFRFMRRQLRRWSHGFIQNVTLHWRGVLTVPFLRSAVAVA
                     LWDAIVASLVFLIVLPLLIITLRSPWLLLAYFIDAPAILVPLLIGAIPRREFGRTLAS
                     LPAFFVLRLVNGFYFLEAVWSEWFLRRASLIYEKGH"
     gene            1965..2333
                     /locus_tag="VEM33_02700"
     CDS             1965..2333
                     /locus_tag="VEM33_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50768.1"
                     /translation="MNPGLPGAGIGGLFYVLSALWMPICELSRLRRGDARGQWPLVAR
                     QFAIAVGIIVAMSGVFWALDTVLMLQQIAAHAAGQMHAMWTLRVSALAVTSGVLATVL
                     GTVHVVRLWLRLRTVRHAAR"
     gene            2330..>2800
                     /locus_tag="VEM33_02705"
     CDS             2330..>2800
                     /locus_tag="VEM33_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS50769.1"
                     /translation="MRACRSAALLVLLLVAWPPPSEAQAAAQGLLQRADAAYSSGNRE
                     LAKTLYRAVLASDPSNSRAAFQLAHLSPPGSAEAVVLLQRYVKLEPGDPWGHMVLGDA
                     LAKAGKVDEAIEQYGLARRSAPAEPDVYLGLGKILRDVGRTDELVRNYEEWASRQ"
BASE COUNT          448 a          890 c          951 g          511 t
ORIGIN      
        1 agcgtgaaaa gcgcgaagag agcggcgccg aagcgggtcg ggatgacggg catgccgaca
       61 ggatgccgca gcggcgtctt ccgccgcaag gcttgcagac ttaccgctcg ctgacgccga
      121 gcctttgagg cagggcgagg atcgcgtcgc ggttggtcca ggagatcgtg agcgcggcgg
      181 cctcgcgcca gggcagccat tcgtagctca ggtgctcggc cgggtcgagc ttcacgggga
      241 ggggctcggg cagcgagagg ctgaacacgc gctcgaggtt gtgcgtgatc cccggagcat
      301 agcggctgcg gtgcttcttg aagatctcga agcggttctc gatgccccag tcgcgcagat
      361 cgtagcgccc cgcatcgagg ccggtctcct cgcgcacctc gcggatcgcg gcgtcgcggg
      421 gggactccga ctcgtgcgcg aggctgcccg tgaccgactg ccagaaaccc ttccagccgg
      481 cgcgctccat catcagcacc tgcaggtccg gggtgtggat caccacgagc accgagcagg
      541 gaattttgta aacagtggtc atggggcggc gggccgggaa tgatagtgca aaggagaaat
      601 ccatgattac gccgccgatg gaaccctttt gcgccggcga gcgtgtcaag ggaatggtgc
      661 tcggagcatt agccggcggg ttttccttcg tcccacggca tcgtcgagaa atgtcgcttg
      721 tcgacgacac agcgcatgcc cgcgccctga cttgtcgcga ttctgtgaag aatgtccggg
      781 ggcaacggct gatcgacagt ttgccgcgcg cataatttgg ccctggtatc agccgcaggg
      841 aatgtcggat ggagatggag aacgtcgtca ccctcacccg ccgccatccc tcccgcgtga
      901 atcttcggcg cgaatcaagc gtgaccgctg cggatctggt catgggcttg accgtcatca
      961 tccccgccta caacgaggcc gggttcgtcg gcgacacaat ccggagcctg ctcgcgcaaa
     1021 cggtcccgcc cgaccagatc atcctggtcg acgacggctc gaccgacggc accggcgatg
     1081 tggggcgccg cgccggcacc acggtgatcc ggcccccacg caacaccgga tccaaggcgg
     1141 gcgcgcaaac cttcgctttg ccgtgggtgc gaaccaagct cgtgatggcg atcgacgccg
     1201 acaccacgct ggagccgacc gccgtcgaaa ggctgatgcg gtcgttcgag gatccgggcg
     1261 tcggcgcggc ctgcgggttt gtcctgccgc gccggatcaa gaccctgtgg gaacgcggtc
     1321 gcttcgtcga atacctgttc gctttcacgt tctacaagca ggtgcaggag tactaccgcc
     1381 gaccgatgat ctcgtcgggc tgcttctcga tgtatcgaac cgacctgatg cgccgccacg
     1441 gcggatggcc gggcaggacg ctggccgagg acatggatct cacctggacc tttcaccaga
     1501 ccggcgtcgg ggtgcgcttc gagcccgagg cggtctgcta cccgatcgag ccgtacaact
     1561 ttcgcttcat gcgcaggcag cttcgccggt ggagccacgg cttcatccag aacgtcaccc
     1621 tgcattggag aggcgtgcta accgtgccgt tcctgcgatc ggcggtggcc gtggccctgt
     1681 gggacgccat cgtagcctcg ctcgttttcc tgatcgtgct gccgctgctg atcatcacgc
     1741 tacggagccc gtggctgctg ctcgcgtatt tcatcgacgc tcccgcgatc ctcgtgccgc
     1801 tcctgatcgg cgcgattccg cgccgcgagt tcgggcgcac gcttgcgagc ctgcctgcat
     1861 ttttcgtgct gcgtctcgtt aacggattct attttctgga agccgtctgg tccgagtggt
     1921 tcctgcggcg cgcctccctg atctacgaga aaggacacta gaccatgaac ccaggattgc
     1981 ccggagccgg aatcggcggc ttgttctacg ttctcagcgc gctctggatg ccgatctgcg
     2041 agctgtcgcg cctgcggcgg ggcgacgcgc gggggcagtg gcccttggtg gcgaggcagt
     2101 ttgcgatcgc ggtcggaata atcgtcgcca tgagcggtgt tttctgggcc ttggatacag
     2161 tgctcatgct ccagcaaatc gccgcgcatg ccgcaggcca gatgcacgcg atgtggactt
     2221 tgcgcgtgtc cgccctggcg gtgacgtcgg gcgtgctggc cacggtgctc ggcacggtgc
     2281 acgtcgttcg gctgtggctg cggctgcgta cggtgcgaca cgctgctcga tgagggcatg
     2341 caggagcgcc gcgctcctgg tcctgctgtt ggtggcctgg ccgcctccga gcgaggccca
     2401 ggcggcggcg caaggcttgc tgcaacgggc cgacgcggct tattcatcgg gaaatcgcga
     2461 gctcgcgaag accttgtacc gggcggtgct cgcgtccgat cccagcaact cccgcgccgc
     2521 ctttcagttg gctcacctct cgcctccggg ctcggccgaa gccgtcgtgc tcctgcagcg
     2581 ttacgtgaag ctcgagccgg gggatccatg gggccacatg gtcctgggcg acgcgctggc
     2641 gaaagccggc aaggtggacg aagcgataga gcagtacggc ctcgcccgcc gcagtgcacc
     2701 ggccgagccg gacgtctact tgggcctggg gaagatcctg cgcgacgtgg gacgcaccga
     2761 cgagctggtg aggaactacg aagaatgggc gtcacgccaa
//
LOCUS       DASPZD010000139         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1048248, whole genome shotgun sequence.
ACCESSION   DASPZD010000139 DASPZD010000000
VERSION     DASPZD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048248"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VEM33_02710"
     CDS             complement(<1..592)
                     /locus_tag="VEM33_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50770.1"
                     /translation="MKRSRIGRAARTLVFVSLALSVSLAAAQSYPSKPIRLLLPFAGG
                     TDAVARLLAFKLSPALGEQVLPEQRLGAGGNIAHRAAAEAAPDGYTLLLGAPPLVINP
                     HLNPTAGYDALRAFAPIAPLTSIANVLVVHPKVKAASLAELLKRAREAPGKLTYGSGG
                     VGSVNQLAAELLKSRAKIDILHVPYKSATTALTGLIA"
     gene            731..1459
                     /locus_tag="VEM33_02715"
     CDS             731..1459
                     /locus_tag="VEM33_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HYS50771.1"
                     /translation="MQSLGIHYYPLTLPFLLALLCVLVALTFLAVLRVLQFAYSQLGI
                     APGYFFTWLFLSLAGSYVNLPIMRFAPEQMTRLEEVPFYGIPYVIPTVETWPGTILAV
                     NVGGAVIPVLLSLVLIAKNRLWKDAVWGVLVVSLACYLLAEPVPGLGIAIPVFYPPVI
                     AATVALLLSRRYAAPLAYACGSLGTLIGADLLNLGNLRGLGAPVVSIGGAGTFDGIFV
                     TALFAVVLAGILTRRKLAQARQRT"
     gene            1456..>2515
                     /gene="gshA"
                     /locus_tag="VEM33_02720"
     CDS             1456..>2515
                     /gene="gshA"
                     /locus_tag="VEM33_02720"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HYS50772.1"
                     /translation="MTGAASFDARLAALSPALLKGLKRGIEKESLRVRPDGALALTPH
                     PRALGSALKHPHITTDFSEALIELITGVHTEVAACLEEMKRIHQFVMRRIGDELLWGA
                     SMPCTLPADENAIPIARYGTSNVGRAKTVYRTGLSHRYGRRMQMIAGLHYNFSLPDST
                     WPLLKLGGPNEAYFALIRNFRRHAWLLLYLFGASPAVCSSFVEGRKHALEELAHGTLY
                     LPHATSLRMGRLGYLSEAQDSLAVSYNSIESYTASLYDVLTKPYPPYEAIGIRDAEGN
                     YRQLATSLLQIENEFYSTIRPKRVIRPGERPLHALRERGVEYVEVRLMDLDPFSPIGI
                     GAPTMRCLDLLLLHCLRAD"
BASE COUNT          378 a          924 c          794 g          419 t
ORIGIN      
        1 cggcgataag gccggtcagc gcggtggtcg cgctcttgta gggcacgtgc aggatgtcga
       61 tcttcgccct ggatttcagc agctccgccg ctagctggtt caccgagccc acgcctccgg
      121 agccgtaagt gagcttgccc ggcgcctcgc gcgcgcgctt gaggagctcc gcgaggctcg
      181 ccgccttcac cttcgggtgc accacgagca cgttggcaat cgaggtgagc ggcgcgatgg
      241 gcgcaaaggc gcgcagcgcg tcgtagccgg ccgtgggatt cagatgcgga ttgatgacga
      301 gaggcggcgc gcccagcagc agcgtgtagc cgtcgggcgc ggcttcggcc gccgcgcggt
      361 gcgcgatgtt cccgcccgcg ccgaggcgct gctcgggcag cacctgctcg ccgagcgcgg
      421 gagacaactt gaacgcgagc aggcgcgcca ccgcgtcggt gccgcccgcg aacggcagga
      481 gcaggcggat cggcttggac ggatagctct gcgcggcggc gagggaaacc gagagcgcaa
      541 gcgacacgaa gacgagcgtt cgagccgcac gaccgatccg agagcgtttc aacgtgcacc
      601 ctttccgggc aagcccgagc cgaattcgac aacgcagcat tgtagcgggc gcgctgcttc
      661 cgggcgataa tcccacccgg actcttccca accgcttcgc ccccgcagtt cccgattcgc
      721 cggagccacc atgcaatcgc tcgggattca ctactatccg ctgacgctcc cgttcctgct
      781 cgcgctcctc tgcgtgctcg tcgccctgac tttcctcgcc gtcttgaggg tgctgcagtt
      841 cgcctattcc cagctcggca tcgcgccggg gtacttcttc acctggcttt ttctctccct
      901 cgccgggagc tacgtcaacc tgcccatcat gcgcttcgcc ccggagcaga tgacgcgcct
      961 ggaggaagtg ccgttctacg gcatacccta cgtgatcccc acggtggaga cctggccggg
     1021 gacgatcctc gcggtcaacg tcggcggagc ggtcattccg gtcctgctgt cgctcgtcct
     1081 gatcgcgaag aaccgtctct ggaaagacgc ggtctggggc gtcctggtcg tctccctcgc
     1141 ctgctacctg ctcgccgagc cggtcccggg cctgggcatc gcgattcccg tcttctaccc
     1201 gcccgtgatc gcggcgaccg tcgcgctgct cctctcgcga cgctacgccg cgcctctcgc
     1261 ctacgcctgc ggaagcctgg gcaccctgat cggcgcggac ctcctgaatc tcggcaactt
     1321 gcgcggcctc ggagcgccgg tcgtgtcgat cggcggtgcg ggaaccttcg acggtatatt
     1381 cgtcaccgcg ctcttcgcag tcgtcctcgc cggcatcctg acccggcgca agctcgctca
     1441 agcgaggcag cgcacgtgac gggcgccgcc tccttcgacg cgcgcctcgc cgcgctctcg
     1501 cccgccctgc tgaagggatt gaagcgcggg atcgagaagg agagcctgcg cgtgcggccc
     1561 gacggcgcgc tcgcgctgac cccgcacccg cgcgcgctcg gctcggcgct caagcatccg
     1621 cacatcacca ccgacttcag cgaagcgctg atcgagctga tcaccggcgt tcacaccgag
     1681 gtcgcggcgt gcctggagga gatgaagcgc atccaccagt tcgtcatgcg caggataggc
     1741 gacgagctgc tgtggggcgc gagcatgccc tgcaccctgc ccgccgacga gaacgcgatc
     1801 ccgatcgcgc gctacggaac gtcgaatgtc ggccgcgcga agaccgtcta ccgcacgggc
     1861 ctctcgcacc gctacggccg gcgcatgcag atgatcgcag gcctgcacta caacttctcg
     1921 cttcccgatt ccacctggcc gctcctcaag ctgggcggcc ccaacgaggc ttatttcgcg
     1981 ctcatccgca atttcaggcg gcacgcctgg ctgctgctgt acctcttcgg cgcctccccc
     2041 gctgtgtgct cgagcttcgt cgaggggcgc aagcacgcgc tcgaggagct cgcgcacggc
     2101 acgctctacc tgccgcacgc cacatccctc cgcatggggc gcctgggcta cctgagcgaa
     2161 gcgcaggact cgctcgcggt gagctacaac agcatcgaga gctacaccgc ctcgctctac
     2221 gacgtgctca ccaagcccta cccgccctac gaggcgatcg gcatccgcga cgccgaaggc
     2281 aactaccgcc agctcgcgac cagcctcctg cagatcgaga acgagttcta cagcaccatc
     2341 cggccgaagc gcgttatccg cccgggcgag cggccgctgc acgcgctgcg cgagcgcggc
     2401 gtggagtacg tcgaggtgcg gctgatggac ctcgacccgt tctcgccgat cggcatcggc
     2461 gcgccgacca tgcgctgcct cgaccttctt ctgctccact gcctgcgtgc cgaca
//
LOCUS       DASPZD010000140         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_459839, whole genome shotgun sequence.
ACCESSION   DASPZD010000140 DASPZD010000000
VERSION     DASPZD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459839"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VEM33_02725"
     CDS             <1..495
                     /locus_tag="VEM33_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYS50773.1"
                     /translation="ARNFKQWMSKGLYSGTVMVGVEQTLLGGKFPWTLHHGHADHECL
                     RPASECRPIDYPKPDGKLTFDRLSSVFISNTNHEENQPCHLVLKDAAVPVAVNLTKYA
                     GPESRYCPAGVYEFVKNGDGSDRLQINAQNCVHCKTCDIKDPAQNIVWVTPQGGDGPN
                     YPNM"
     gene            564..1172
                     /locus_tag="VEM33_02730"
     CDS             564..1172
                     /locus_tag="VEM33_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYS50774.1"
                     /translation="MRKGEQTRTLILNEAVALASQVGLEGLSIGSLAGRLAMSKSGLF
                     AHFGSKEDLQLLTLKQAQTLFFEQVLSPALKQARGLPRLRALFANWLAWVERNDDRPG
                     GCLMLAASVEYDDRPGAVRDLLVAGQRELRGAIVKAIRIAIDEGHLGSQTDPWQLSFE
                     LFGIVLATHHDLRLLGDARALGRARDTLERLLEAHRPTAPGV"
     gene            1181..1780
                     /locus_tag="VEM33_02735"
     CDS             1181..1780
                     /locus_tag="VEM33_02735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYS50775.1"
                     /translation="MRSRDATAPEFSAAYPAHLVASLPLGVGHSITVRPIRPEDIDLE
                     TDFAHKLSKETRYNRFLGGGVRLTPEMLEKFTRIDFSRDMALIATTTIDGAETAIGVT
                     RYARLADDVTCEFAITVADAWQGCGIGRKLLAMLVDAARGHGLRRIIGDVLATNTPML
                     RLAHSQGFRIERHPEGAELRRVVLELVVDPPRIPISAGS"
     gene            1813..>2212
                     /locus_tag="VEM33_02740"
     CDS             1813..>2212
                     /locus_tag="VEM33_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50776.1"
                     /translation="MKTSRIELWTLLFVFAFFPAAAEAQNVFRLYGGLAPTSYKISFD
                     QNAPVGYSNQTAKSSYTAANVGVTWISPKGIYVDFAASQSLSATHDLWNSVPNTPPQD
                     FSHDTYTLTGGYSHAFAQGVSVSGFGGYTSS"
BASE COUNT          431 a          735 c          655 g          391 t
ORIGIN      
        1 gcgcgcaact tcaagcagtg gatgtccaag gggctttact ccggcaccgt gatggtcgga
       61 gtcgagcaga ccctgttggg tggaaaattt ccctggaccc tccaccacgg ccacgccgac
      121 cacgaatgtc tccggcccgc atcggaatgc aggccgatcg actatccgaa gcccgatgga
      181 aaactcactt tcgatcgcct ctcctcggta ttcatctcca ataccaacca cgaggagaac
      241 cagccctgcc acctggttct caaggacgcg gccgttcccg tcgccgtgaa cctcacgaaa
      301 tacgcgggcc ccgaaagccg ctactgtccg gcgggcgtat acgaattcgt gaaaaacggc
      361 gacggcagcg accgcctcca gatcaacgcg cagaactgcg tccactgcaa gacctgcgac
      421 atcaaggacc cggcccagaa catcgtctgg gttacgccac agggtgggga tggaccgaac
      481 tacccaaaca tgtgacctgc tcttaccccc tcaaaagaaa cgagatagtt gcagacaacg
      541 aacgttcgta ctatcgtgtg cagatgcgaa aaggcgagca gacgcgcacg ttgatcctca
      601 acgaagccgt cgccctcgca agccaggtcg ggctggaggg actgagcatc ggctcgctcg
      661 ccggacggct ggccatgtcc aagagcgggc tcttcgctca tttcggctcc aaagaagact
      721 tgcagctcct gactctcaag caggcgcaga cgctgttctt cgagcaagtt ctctcacctg
      781 ctttgaaaca agcccgcggc ctgccgcgtc tcagggccct gttcgcgaac tggctggcgt
      841 gggtcgaaag gaacgacgat cgcccggggg gctgcctgat gctcgccgct tcggtcgagt
      901 acgacgacag gcccggggcg gtgcgcgacc tgctcgtcgc cggacagcgc gagctgcgcg
      961 gcgcaatcgt caaggcgatc cgcattgcga tcgatgaagg acatctcgga tcccagaccg
     1021 atccctggca gctcagcttc gagcttttcg ggatcgtcct ggcaactcat cacgacttga
     1081 ggctgctcgg ggatgcgcgc gcactcggcc gcgcgcgcga caccctggag cggctgctcg
     1141 aagcgcatcg cccgacggca ccgggcgtct gaaggccgcc gtgcgctccc gggacgccac
     1201 ggcgccggag ttctccgcgg cctatcccgc gcacttggtc gcgtccttgc ccctgggggt
     1261 cgggcactcg atcacggttc gcccgatccg ccccgaggac atcgacctcg agaccgattt
     1321 cgcgcacaag ctctcgaaag aaacacgcta caaccgcttc ctcggcggcg gtgtccgact
     1381 cacgccggag atgctggaaa aattcacccg catcgatttc agccgcgata tggcgctgat
     1441 cgcgacgacg actatcgacg gagccgaaac cgcgatcggc gtgacgcgct acgcgcgtct
     1501 cgcggacgac gtcacctgcg aattcgcgat caccgtcgcc gatgcctggc agggctgcgg
     1561 catcggcagg aagctcctcg ccatgctcgt cgacgcagcc cgcgggcacg gcttgcggcg
     1621 gatcatcggt gacgtgctcg ccacgaacac gcccatgttg cgtctcgcgc actcgcaggg
     1681 gtttcgcatc gagcggcatc ccgagggcgc ggagctgcgc cgcgtggtgc tcgagcttgt
     1741 cgttgacccg ccgcgcattc caatttctgc gggaagctga cacaattgcg caacagagga
     1801 ggcccacttg tcatgaaaac atcaagaatc gagctgtgga cgttgctttt cgtcttcgcc
     1861 ttttttcccg ctgcggcgga ggcccagaac gtgttccgtc tatacggcgg gctcgcaccg
     1921 accagctaca agatttcgtt cgaccagaac gcccccgtcg gctatagcaa tcagactgcg
     1981 aagtccagct acacggcggc caatgtcggc gtgacctgga tatcgcccaa gggaatctat
     2041 gtcgacttcg cggcttcaca gagcctcagc gccacgcacg acctgtggaa ttccgtaccc
     2101 aacaccccgc cgcaggattt ttcgcacgat acttacacgc tgacgggggg ctacagccat
     2161 gcttttgccc agggcgtgag cgtctcgggt tttggcggct acacatcgag ca
//
LOCUS       DASPZD010000141         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1272919, whole genome shotgun sequence.
ACCESSION   DASPZD010000141 DASPZD010000000
VERSION     DASPZD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272919"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VEM33_02745"
     CDS             <1..673
                     /locus_tag="VEM33_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYS50777.1"
                     /translation="GQKCTAIRRVLVPQAHATTVRDDLAEGLKAVRVGNPMLEETRMG
                     PLASGSQLADVREGVAKLGVEGKPAFGSAAPLAGKGFFVSPVLMEFEKDALAAAVHAR
                     EVFGPVASLLPYSGKGADAAAIVARGNGGLVSSVYSEDAVFLEEIVRGIAPYHGRIFL
                     GHPKIEMSPGPGTVLPQLVHGGPGRAGGGEELGGARGLAFYMQRVALEGSRPVIQKLI
                     GVQPS"
     gene            complement(670..1689)
                     /locus_tag="VEM33_02750"
     CDS             complement(670..1689)
                     /locus_tag="VEM33_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYS50778.1"
                     /translation="MMRLPWFGFHSPRSIVEAAKILAAEGPQAMLIAGGTDLLPNMKR
                     RHQTPATLVSLRRIEALKKISNGSGSTLCAGLTLNEIVANAAVREKYAGLWQAAAQVA
                     TVHLRNMGTLGGNLCLDTRCNYYNQSYEWRKAIDFCLKKDGEICWVATASKRCVAASS
                     TDTAPALMSLGAIVHLVSTQGERDVALADLYRNDGIDYLTRRPDEILTEVKLRASEGW
                     RSSYWKLRRRGAFDFPVLGVAAAVKTTRDGTVEDARIALGAVSSWPFLTKGGELLVGT
                     KLSDDAIAEAGAKVASAAKPMDNTDLDLYWRKAVVAEFVGYALREIRGDDMRATRVRI
                     ARHVL"
     gene            complement(1686..>2919)
                     /locus_tag="VEM33_02755"
     CDS             complement(1686..>2919)
                     /locus_tag="VEM33_02755"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYS50779.1"
                     /translation="LVPCVQAVVDASGWRSRFRKLGRGRGLGIAASSYLCGAGLPIYW
                     NEMPHSGVQLLLDRSGQVTVFCGATEIGQGSDDVLAAIVAEVLGIDAYDVRCVTGDTG
                     LTPVDLGSYSSRVTVMMGNAAIQAAERLRDLIARAAAEHMETLPGNVGFAQGRVFRAD
                     DPGKLMSFREAIVLAEEKFGTLGSVGSYSPPRAPGRYKGAGVGPSPAYSYTACVVEAE
                     VDRKTGWITVPKVWIAHDIGRAINPVSARGQVEGSVYMGLGEALMEEQVFRRLPPKLS
                     NALVHKIPSLLEYKSLTSLDMPEVFTELVEDPDPNCPFGAKEVGQGPLLPVMPAVANA
                     VYDAIGVRIDELPITPDKVLAALEKKAKGETPRIGPESFPDVPYPEPMLVPPPWEGGD
                     GNSVNTPRRRPVKKEALA"
BASE COUNT          451 a          992 c          977 g          499 t
ORIGIN      
        1 gggccagaaa tgcaccgcga tccggcgcgt gctcgtgccg caggcgcacg ccaccacggt
       61 gcgcgacgac ctcgccgagg gcttgaaagc cgtgcgcgtg ggcaatccta tgcttgagga
      121 aacgcgcatg ggaccgcttg cctcgggcag ccaactcgcc gacgtgcgcg aaggcgtcgc
      181 caagctcggc gtcgaaggca agcccgcgtt cggcagcgcg gcgccgctcg ccggcaaggg
      241 attcttcgtc tcccccgttc tgatggagtt cgaaaaggac gcgctcgcag ccgcagtgca
      301 tgcgcgcgag gtgttcggcc cggtggcttc tctgctgccc tactcgggca aaggcgccga
      361 tgccgccgcg atcgtcgcgc gcggcaacgg cggcctggtg tcatcggtct attcagagga
      421 cgcggtgttt ctggaagaaa tcgtccgggg catcgcacct tatcacgggc ggatcttcct
      481 cggccatccg aagatcgaga tgtcgccggg gccggggacg gtgctgcccc agctcgtgca
      541 cggcggcccg gggcgcgcgg gtggcggcga ggagctgggc ggcgcgcgtg ggcttgcgtt
      601 ctacatgcag cgcgtggcgc tggaaggctc gcggccggtg atacagaagc tgatcggcgt
      661 ccagccctcc tagagcacgt gccgcgcaat gcgaacgcgt gtcgcgcgca tgtcgtcgcc
      721 gcggatttcg cgcagcgcgt agcccacgaa ttccgccacc accgcttttc tccagtagag
      781 atccaagtcg gtgttgtcca tcggcttcgc cgccgatgcg accttggcgc cggcttcagc
      841 aatggcgtcg tcgctgagct tcgtgccgac gagaagctcg ccgcccttcg tgagaaaagg
      901 ccacgaagag accgcgccga gcgcgatacg cgcgtcttcg accgttccgt cgcgcgtcgt
      961 cttcaccgcc gcggcgactc cgagcacggg gaaatcgaag gccccgcggc ggcgcaactt
     1021 ccagtagctg ctccgccatc cttccgaggc gcgcagcttc acctcggtga ggatctcgtc
     1081 gggccggcgc gtgagatagt cgatgccgtc gttcctgtag agatcggcga gcgccacgtc
     1141 gcgctcgcct tgcgtggaga cgaggtgcac gatcgcgccc agggacatca aagcgggtgc
     1201 cgtgtcggtc gaggaagccg caacgcagcg cttgctcgcg gtggcgaccc agcagatctc
     1261 gccgtctttt ttcaggcaga agtcgatcgc cttgcgccac tcgtagctct ggttgtagta
     1321 gttgcagcgc gtgtctaggc acaggttacc gccgagcgtg cccatgttgc gcaggtgaac
     1381 ggtcgcgact tgagccgcgg cctgccacag ccccgcatat ttttcgcgca ccgccgcgtt
     1441 cgcgacgatt tcattcagag tcagtcccgc gcagagcgtg ctgccggagc cattggatat
     1501 tttcttcagc gcttcgatcc ggcgcagcga tacgagcgtc gccggagtct ggtggcgccg
     1561 tttcatgttg ggcaggagat ccgtcccgcc cgcgattaac atagcctgcg gaccctcggc
     1621 ggcgaggatc ttcgccgctt cgacgatgga cctcggcgaa tgaaatccga accagggcag
     1681 ccgcatcatg ccagggcttc cttcttcacg ggtctgcgtc gcggcgtgtt gacggaattg
     1741 ccgtcgccgc cttcccaggg cggcggcacc agcatgggct cggggtaggg cacatcggga
     1801 aagctctccg gcccgatgcg cggcgtctcg cccttggctt ttttctcgag cgccgcgagc
     1861 accttgtccg gggtgatggg caactcgtcg atgcgcaccc cgatcgcgtc gtacacggcg
     1921 ttcgcgaccg cgggcatcac gggcagcaaa ggcccctgcc ccacctcttt tgccccgaac
     1981 gggcagttgg ggtccggatc ctcgacgagc tcggtaaaga cttcgggcat gtccagcgag
     2041 gtcaggctct tgtattcgag gagcgaagga atcttgtgca caagtgcgtt ggaaagcttg
     2101 ggcggcaggc gccggaacac ctgctcttcc atcagggctt cgcccaggcc catgtagacg
     2161 ctgccttcga cctgaccgcg cgcgctcacc gggttgatgg cgcgcccgat gtcgtgcgcg
     2221 atccacacct tcggcaccgt gatccagccc gtcttgcggt ccacttcggc ttcgaccacg
     2281 caggccgtat aggagtacgc gggcgagggc ccgacccccg ctcccttgta ccgcccggga
     2341 gcgcgcggcg gggaatacga gcccaccgag ccgagcgtcc cgaatttttc ctcggccagc
     2401 acgatcgctt cgcggaagct catcagcttg ccgggatcgt ccgctcggaa cacccggccc
     2461 tgcgcgaagc caacgttccc gggcagcgtt tccatgtgct cggcggccgc tcgcgcgatc
     2521 aggtcgcgca ggcgctcggc cgcctggatc gcagcgttgc ccatcatcac ggtgacgcgg
     2581 ctggaatacg acccgaggtc caccggcgtc aggccggtgt cgccggtgac acagcgcacg
     2641 tcataggcgt cgatgccgag cacctcggcg acgatcgccg cgagcacgtc gtccgagccc
     2701 tggccgatct cggtcgcgcc gcagaacacg gtcacctggc cgctgcggtc gagcagcagc
     2761 tgcacgccgc tgtgcggcat ctcgttccag tagatcggca ggcccgcgcc gcacaggtac
     2821 gacgacgcgg cgatgcccag gcctcgcccg cgcccgagct tgcggaaccg cgagcgccag
     2881 ccggaggcat cgacgacggc ctgcacgcac ggcaccagg
//
LOCUS       DASPZD010000142         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1147193, whole genome shotgun sequence.
ACCESSION   DASPZD010000142 DASPZD010000000
VERSION     DASPZD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147193"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VEM33_02760"
     CDS             <1..524
                     /locus_tag="VEM33_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS50780.1"
                     /translation="RGERIEAQRPDRIARCGIALVPEGRQIFPNLSVRENLVAFAENR
                     SATRDPWTLAKVLAFFPRLVGRMDNLGSRLSGGEQQMLAIGRALMTNPHLLILDEATE
                     GLAPLVREEIWRCLAALKAAGQTILVIDKYVERLVAIADHHSILSRGRVAWRGSSAEL
                     GADRSLWHRYLGV"
     gene            601..1926
                     /locus_tag="VEM33_02765"
     CDS             601..1926
                     /locus_tag="VEM33_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYS50781.1"
                     /translation="MLHERIPNLAKFVEPDRVRREVYTDAAIFEMEMERIHERVWIYC
                     GHETQVPKAGDYYATQIGRQPMLMVRGKDGKVNVLYNRCPHRGSMMCGDRSGNAGEFF
                     RCSYHSWTFHHDGKLRAIPMMESGYAGTRWTRDNPDCNMKRAARVENYRGFVFASLAT
                     EGPGLLEFLGRSKVAFDDICDRSPEGQVEVVPNCFRMVQHSNWKIFLENQLDALHPSV
                     THQSTGRAAREVELEIEKRTGKIPLSYHMLSAFSTVTIEKWDNFQTLNYPYGHCILTG
                     YMGLRPQDPETLEYERVLAKAYGEKRKEEILSTNIHHVLIYPGLSVQSPLQQLRAVRP
                     LDVDKTLTEIWHFRLKGAPEPIYRRALAYYNLVNSPSTLINADDLENFWKAHQGLSSD
                     GGDWVSFHRHYGRDAEKDGIVETAPNCGTSEAPMRNQMKAWTQYMDSGK"
     gene            1929..2480
                     /locus_tag="VEM33_02770"
     CDS             1929..2480
                     /locus_tag="VEM33_02770"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HYS50782.1"
                     /translation="MAKRTARKAVAARRAVRAAKAPPNGRDTQHAVEQFLYRQAELLD
                     EKRWQDYIDLFAEDGMYWMPPEPSHATWDGMPAIFAEDRNLMTVRMKRVLHPDAWSQR
                     PLWGTNHVVGNVVVERESPNGDVVARSRFHMMELRRDDVRHFAGLYIHHLKKTKDGYR
                     IKLQRVDMTNAQAAYDYVLQVWV"
     gene            2543..>3430
                     /gene="dctP"
                     /locus_tag="VEM33_02775"
     CDS             2543..>3430
                     /gene="dctP"
                     /locus_tag="VEM33_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HYS50783.1"
                     /translation="MTRFAPRLALAAALAAISGIACAQEITLRLVSAFPENQFYVKRT
                     LDWIADFNKDGKGVLQINFIGGPKAIPTFEVGKAVQSGVVDIGFSTGAFYTNVMPESD
                     ILKLAETSAAEQRKNGGFDLINKIWAEKGNMRYLAKVVEFTPFHLYINKKIDKPDLTG
                     MKIRITPVYREFFQSMNAQVMTTAPGEVYTALERGVIDGYGWPIHALFDLNWQEKTKY
                     RVDPGFYNAEVALIINLDKYKSLTPAQRDYLDRKARAYEAQNDFWKTYNQSEAKRQAE
                     AGIQVIAFDAATSKAYVEKA"
BASE COUNT          675 a         1135 c         1092 g          528 t
ORIGIN      
        1 tccgcggcga gcgcatcgag gcgcagcgcc ccgaccgcat cgcgcgctgc ggcatcgcgc
       61 tcgtcccgga aggacgccag atcttcccca acctgagcgt gcgcgagaac ctggtcgcgt
      121 tcgcggaaaa ccgcagcgcg acgcgcgacc cgtggacgct tgccaaggtg ctggcattct
      181 tcccccggct cgtcgggcgc atggacaacc tcggcagccg gctctcgggc ggcgagcagc
      241 aaatgctcgc catcgggcgg gcgctcatga ccaatccgca cctcctcatc ctcgacgagg
      301 cgaccgaggg gctcgcgccg ctggtccgcg aggagatctg gagatgcctc gccgcgctga
      361 aggcagccgg ccaaacgatc ctggtgatcg acaagtacgt ggagcgtctc gtcgcgatcg
      421 ccgaccatca cagcatactc tcgcgcggtc gcgtcgcttg gcgcggctca tcggccgagc
      481 tgggtgccga tcgctcgttg tggcaccgct atctcggcgt atgaccctcg cgccttgaaa
      541 aaacggcgct ccggcggtat cgttcccttc cccggaagac cgcgctccaa ggagcccgca
      601 atgctgcacg aacgcatccc caatctcgcc aagttcgtcg agcccgatcg tgtgcggcgc
      661 gaggtgtata cggacgcggc gatcttcgag atggagatgg agcgcatcca cgaacgcgtt
      721 tggatctact gcggccacga gacgcaggtg ccgaaggcgg gcgattacta tgcgacgcaa
      781 atcggccgcc agccgatgct tatggtgcgc ggcaaggacg gcaaggtgaa tgtcctgtac
      841 aaccgctgcc cgcaccgcgg ctcgatgatg tgcggcgacc ggagcggcaa cgcgggcgag
      901 ttcttccgct gctcgtacca ctcctggact ttccaccacg acggcaagct gcgcgccata
      961 ccgatgatgg agtccggcta cgccggcacg cgctggaccc gagacaatcc cgactgcaac
     1021 atgaagcgcg cggcgcgggt ggagaactac cgcggcttcg tgttcgcgag cctcgccacc
     1081 gaaggtccgg ggctgctcga gtttctcggg cgctcgaagg tcgccttcga cgacatctgc
     1141 gaccgctcgc ccgaaggcca ggtcgaggtc gtgccgaact gcttccggat ggtccagcac
     1201 tccaactgga agatcttcct ggagaaccag ctcgacgccc tgcatccctc ggtcacgcat
     1261 cagtccacgg gacgcgcggc gcgggaagtg gagctggaga tcgagaaacg caccggcaag
     1321 atcccgctgt cgtatcacat gctgtcggcg ttctcgaccg tgacgatcga gaagtgggac
     1381 aacttccaga cgctcaacta cccgtacggc cactgcatcc tgaccggcta catgggcctg
     1441 cggccgcagg acccggaaac gctcgagtac gaacgcgtgc ttgccaaggc ctacggcgaa
     1501 aaacgcaagg aggaaatact ctccaccaac atccatcacg tgctcatcta tcctggcctc
     1561 tcggtgcagt cgccgctgca gcaattgcgc gcggtgcggc ccctggacgt ggacaagacg
     1621 ctgaccgaga tctggcattt ccgcctgaaa ggcgccccgg agccgatcta ccggcgggcg
     1681 ctcgcctact acaaccttgt caattcgccc tcgacgctga tcaatgccga cgacctggag
     1741 aacttctgga aggcgcatca gggcctttcc tccgacggcg gtgactgggt gagtttccac
     1801 cgtcactacg gccgcgacgc ggagaaggac ggcatcgtgg agaccgcgcc caactgcggc
     1861 acgtcggagg cgcccatgcg caaccagatg aaggcctgga cgcagtacat ggactcgggg
     1921 aagtagcaat ggcgaagcgg actgcgagga aggccgtggc ggcgaggcgg gcggtccgcg
     1981 ccgcgaaggc gccgcccaac ggccgcgaca cgcagcacgc ggtcgagcag tttctctacc
     2041 ggcaagccga gctcctcgac gagaaacgct ggcaggacta catcgacctc ttcgccgagg
     2101 acggcatgta ctggatgccg cccgagccgt cgcacgccac ctgggacggc atgcccgcga
     2161 tcttcgccga ggacaggaac ctgatgacgg tgcgcatgaa gcgcgtgctg cacccggacg
     2221 cgtggtcgca gcggccgctg tggggtacga accacgtggt cggcaacgtc gtcgtcgagc
     2281 gcgaatcgcc gaacggcgac gtcgtggcgc gctcgcgctt ccacatgatg gagctgcgcc
     2341 gcgacgacgt gcgccatttc gccggcctgt acatccatca tctgaagaag acgaaggacg
     2401 gctaccgcat caagctgcag cgggtcgaca tgaccaacgc gcaggcggcg tacgactacg
     2461 tgctgcaggt gtgggtgtag cggcggccat tcggcgtatc atctcaagca tcccggcaga
     2521 ccctctcaga ggaggagaca gcatgacccg ctttgcaccc agactcgcgc tcgccgcggc
     2581 gctagccgca atttcgggga tcgcctgcgc gcaggagata acgctgcgcc tggtcagcgc
     2641 tttccccgag aaccagttct acgtgaagcg cacgctcgac tggatcgccg acttcaacaa
     2701 ggacggcaag ggcgtcctgc agatcaactt catcggcggg ccgaaggcga tcccgacatt
     2761 cgaggtcggc aaggcggtgc agagcggcgt ggtcgacatc ggcttcagca ccggcgcctt
     2821 ctacaccaac gtgatgcccg agtcggacat cctgaagctc gcggagacga gcgctgccga
     2881 gcagcgcaag aacggcggct tcgacctcat caacaagatc tgggcggaga agggcaacat
     2941 gcgctacctc gccaaggtcg tcgagttcac gccgttccac ctgtacatca acaagaagat
     3001 cgacaagccc gacctcaccg gcatgaagat ccgcatcacg ccggtgtacc gcgaattctt
     3061 ccagtcgatg aacgcgcagg tcatgaccac ggcgcccggc gaggtctaca ccgcgctcga
     3121 gcgcggtgtg atcgacggct acggctggcc gatccatgcc ctcttcgacc tgaactggca
     3181 ggagaagacc aagtatcgcg tcgacccggg gttctacaac gccgaggtgg cgttgatcat
     3241 caacctcgac aagtacaagt ccctgacgcc ggcgcagcgc gactacctcg accgcaaggc
     3301 gcgggcgtac gaggcgcaga acgacttctg gaagacctac aatcagtccg aagcgaagcg
     3361 ccaggccgaa gccggtatcc aggtgatcgc tttcgacgcg gcgaccagca aggcgtacgt
     3421 cgagaaggcg
//
LOCUS       DASPZD010000143         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1272785, whole genome shotgun sequence.
ACCESSION   DASPZD010000143 DASPZD010000000
VERSION     DASPZD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272785"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VEM33_02780"
     CDS             <1..319
                     /locus_tag="VEM33_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50784.1"
                     /translation="ARTLREFDDVVTAPLHGFRDTDDYWTRASAKPVLGRIGVPTLLL
                     NARNDPFLPAAALPRPDEVSAHVQCDFPGEGGHAAFVSGPFPGNLGWMPRRVTAFFAE
                     VPR"
     gene            271..1704
                     /locus_tag="VEM33_02785"
     CDS             271..1704
                     /locus_tag="VEM33_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYS50785.1"
                     /translation="MDAAPGDGVLRGSSPVNLPPQEIFKAYDIRGVVGKSLTREGVKL
                     IGQALGSELLDRSSRGGRPEIAVGRDGRISGPELAQALMEGIRASGVDVAEIGMFATP
                     VAYFAAHELGCGSAVAVTGSHNPPEYNGLKMVLAGTTLAGEEVQRLRKRIESGQLARG
                     AGRHRRVDIREAYLNRITSDVRPARSMKIAVDCGNGVAGATAGELYRRLGCEVLELYC
                     EVDGRFPNHHPDPSHPENLKDLISLVQSKGCEVGFAFDGDGDRLGVVTAEGKIIFPDR
                     QLMLFAKDVLSRQPGAEIIYDVKSTRNLAPWIRRHGGVPSLWKTGHSLIKARMRERGA
                     ALAGEMSGHTFFVERWYGFDDAQYAGARLLEILSKTPDATATLNALPDSMGTPELHLK
                     LAEGEPHRLIETLAKIASFPGATEVIRLDGLRVEYPDGFGLARASNTTPVVVLRFEAE
                     DEAGLDRIKSEFRRVLLSVKPDAKLPF"
     gene            1723..2238
                     /locus_tag="VEM33_02790"
     CDS             1723..2238
                     /locus_tag="VEM33_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYS50786.1"
                     /translation="MTRVPLIEEEEHPELAGLIATIKSERGGRLLHLYRALLNSPPVA
                     EGWLKLFTAIRQKGKLGGRYRELAILRVALLNGAEYEYRAHVPFALKEGVTQEQIDAL
                     PGWQVSKRFDDRERAVLAHTDCMTRGIRVPDPIFAEVRRHFDDRETVELTATIAGYNL
                     VSRFLVAMQID"
     gene            complement(2266..3006)
                     /gene="ugpQ"
                     /locus_tag="VEM33_02795"
     CDS             complement(2266..3006)
                     /gene="ugpQ"
                     /locus_tag="VEM33_02795"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HYS50787.1"
                     /translation="MHRPWPYPRVVGHRGAGTLAPENTLAGIRKAAAMGFGGVEFDVM
                     LSADKVPLLIHDETLDRTTSGRGSVAATPYTRLASLDAGAWFSPEYRGERLPSFEEAG
                     KLCAQLGLWANVEIKPARGFERETAAAVAKLALELWSASPAPLLSSFEPLSLDAARAV
                     APDLDRGYLTDRLAPGWVEAARALGCVSVHCNSKYLTQAQADEVRSAGYWLLCYTVNE
                     PSSARRLFSWGIDAIFTDRLDLIPPDFA"
     gene            3348..>3635
                     /locus_tag="VEM33_02800"
     CDS             3348..>3635
                     /locus_tag="VEM33_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HYS50788.1"
                     /translation="MGTAYFSLLRMLADGRFHSRERLSRALGLSGGGVWNLIRRIEAL
                     GLRVVKVRGRGYRLAEALDLFEPDALVAELKNVSPELCIEVLDECPSTNTVL"
BASE COUNT          649 a         1153 c         1201 g          632 t
ORIGIN      
        1 cgcgcgcacg ctgcgcgagt tcgacgacgt cgtcacggcg cccttgcacg gattccgcga
       61 taccgacgat tactggacgc gcgcgagcgc gaaaccggtg ctcgggcgca tcggggtgcc
      121 gactctgctc ttgaacgccc gcaacgatcc cttcctcccg gctgcagcgt tgccgcgccc
      181 ggacgaggtt tccgcccacg tgcagtgcga ttttcccggt gaaggcgggc acgccgcgtt
      241 cgtcagcggt ccgtttcccg ggaatctggg atggatgccg cgccgggtga cggcgttctt
      301 cgcggaagtt ccccggtgaa tttaccccct caggaaatct tcaaggccta cgatatccgc
      361 ggcgtcgtcg gcaaatcgct cacccgcgaa ggcgtgaagc tgatcgggca ggccctcgga
      421 agcgagctgc tcgaccgcag cagccggggt gggcggccgg agatcgcggt gggtcgcgac
      481 ggccggatct ccgggcccga gctcgcccaa gcgctcatgg aaggcattcg ggcaagcggc
      541 gtggacgtcg cggagatcgg gatgttcgca actccagtcg cctatttcgc ggcgcacgaa
      601 ctgggctgcg gcagcgcggt cgcagtcacg ggcagccaca atccgccgga gtacaacgga
      661 ttgaagatgg tgctcgcggg aaccactctc gcgggcgagg aagtgcagcg gttgcgcaag
      721 cggatcgagt ccggccagct cgctcgcggc gcggggcggc accgtcgtgt cgacatccgc
      781 gaagcctatc tgaatcgcat cacctccgac gtacggcctg cgcgttccat gaaaatcgcg
      841 gtcgactgcg gtaacggcgt cgccggtgcc acggcgggcg agctctaccg ccggctcggc
      901 tgcgaagtgc tggagctcta ctgcgaggtc gatggccgct ttcccaatca tcatcccgat
      961 ccgtcccacc cggagaacct caaggacctg atatccctgg tgcaaagcaa gggctgtgaa
     1021 gttgggttcg ctttcgacgg cgacggcgac cggctgggcg tggtgaccgc cgaggggaag
     1081 atcatttttc cggaccggca gctgatgctg ttcgcgaagg atgtcctctc gcgccagccg
     1141 ggcgccgaga tcatctacga cgtaaagagc acgcgcaacc tcgcgccctg gatccgccgg
     1201 cacggcggcg tgccgagcct ctggaagact ggtcattcgc tcatcaaggc gaggatgcgc
     1261 gagcgcggtg cggcgctcgc cggcgagatg agcggccata cgttcttcgt cgaacgctgg
     1321 tacgggttcg acgacgcgca atacgccggg gcgcgcctgc tggagatcct gagcaagacg
     1381 cccgacgcca ccgcgacgct caacgcactt cccgattcga tgggtacgcc tgaactgcac
     1441 ctgaagctcg cagagggcga accgcatcgc ttgatcgaaa ctctcgcgaa gatcgcctca
     1501 ttcccggggg cgaccgaggt gatcaggctg gacgggctac gggtcgaata ccccgatgga
     1561 ttcgggctcg cgcgcgcctc gaacacgacg ccggtcgtcg tcctgcgctt cgaagcggag
     1621 gatgaagcgg gcctcgatcg catcaaatcc gaattccggc gggttctgct ttcggtgaaa
     1681 ccggatgcga aattgccgtt ctagtgtcgt gaaggaggcg tcttgacacg cgtaccgctg
     1741 atcgaagagg aagaacaccc cgagctcgcc gggctgatcg cgaccatcaa gtccgagcgt
     1801 ggaggcaggc tgctgcacct gtatcgagcg ctgttgaaca gcccgccagt ggccgaaggc
     1861 tggctaaagc ttttcaccgc gatccgccaa aaagggaagc tcggcgggcg ttaccgcgag
     1921 ctggccattc tgcgcgtggc gctcctgaac ggcgccgaat acgaataccg cgcgcacgtg
     1981 cccttcgcgc tgaaggaggg cgtgacccag gagcagatcg atgcgcttcc cggctggcag
     2041 gtttcgaagc gcttcgacga tcgcgagcga gcggtgctcg cccataccga ctgcatgaca
     2101 cgcgggatcc gcgtacccga cccgattttc gccgaagtgc ggcggcactt cgacgaccgg
     2161 gaaacggtcg agctcaccgc gacgatcgcg gggtacaacc tcgtgtcgcg cttcctggtg
     2221 gcgatgcaga tcgattaggg gaacctgcgc tcccgggacg gcagctcagg cgaagtccgg
     2281 tggtatcaaa tccagtctgt cggtgaagat cgcgtcgatg ccccaggaaa acaagcgccg
     2341 cgcgctcgag ggttcattga ccgtgtagca gagcagccaa tagccggcgc tgcggacttc
     2401 atcggcttgc gcctgagtca gatacttgga gttgcaatgc acggacacac agccgagcgc
     2461 gcgcgcggct tcgacccaac ccggcgcgag gcggtcggta agatatccgc gatcgagatc
     2521 cggcgcgaca gcgcgcgccg catcgagcga gagcggctcg aacgacgaca gcaggggtgc
     2581 gggggaagcg ctccataact ccaaagcgag cttcgcgacc gccgcagcgg tttcacgctc
     2641 gaaccctcgc gcgggcttga tctcgacatt ggcccagagg ccaagctggg cgcacagctt
     2701 gccggcttcc tcgaacgacg gcaggcgctc accgcggtat tccgggctga accaggcgcc
     2761 ggcgtccagg ctcgcgagcc ttgtgtaggg cgtcgcggcg acgctgcccc gcccgctcgt
     2821 cgtgcgatcc aaggtttcgt catggatcag caaggggact ttgtccgccg agagcattac
     2881 gtcgaactcc acgccgccaa atcccattgc agccgccttg cgaatacccg ccaaggtgtt
     2941 ctcgggggcg agcgtgccgg cgccgcgatg acccactact cgcggataag gccagggtcg
     3001 atgcatgcgt cgggtcgcag cggaggtgag gtcgggggca ggctactccc attataaaca
     3061 gccgattcag ctgcgatttg cgcggtgcag gtgggaaaaa aaaacggggg aagggagggt
     3121 ttcccccgtt tagccgccgt ttgctgggcc ggcgggcaac ctgagacgcc taccagaact
     3181 cgtcgctggg cgtcatgaaa ttcgctatga cggcatcata tcacctgact cgcttcggaa
     3241 caattgaaaa tcgatcccta gtgacttttt cacgaatcgc gggagcctcc cgcgcatacg
     3301 ctcccccggt gctagtattt gtccggtttt tcctcgcgat tttgcgcatg ggcacagcct
     3361 acttctccct gcttcgcatg ctggccgacg gcaggtttca ctcccgcgag cggctctctc
     3421 gggctctggg gctttccggc ggaggcgtct ggaacctcat caggcggatc gaagcgttgg
     3481 gcttgcgcgt agtcaaggta cgcggccgcg gttaccgcct ggccgaggct ctcgacctgt
     3541 tcgagccgga cgcgctcgtg gccgaactga agaacgtctc gcccgagctt tgcatcgagg
     3601 tgctcgatga atgtccgtcc accaataccg ttctc
//
LOCUS       DASPZD010000144         3806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1147061, whole genome shotgun sequence.
ACCESSION   DASPZD010000144 DASPZD010000000
VERSION     DASPZD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147061"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..465
                     /locus_tag="VEM33_02805"
     CDS             46..465
                     /locus_tag="VEM33_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50789.1"
                     /translation="MKPPDLLRAASVLTLIFCAGHTYGALGSSSADPEQAAVFMAMQA
                     YPFEIMGARRTHWQFYRGFSLLFSVTLLLLAVLLWQLARLSNRDPAGARPLIASLFLA
                     YLGFTILCGVYFFIAPAAFSAAVAICLALAFTSARSG"
     gene            529..2325
                     /locus_tag="VEM33_02810"
     CDS             529..2325
                     /locus_tag="VEM33_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HYS50790.1"
                     /translation="MSAPSPRKQEEASHSNFIRQIIEADLMAGKYATRKWNGKPGSAL
                     SLHRAPPDPAKIRTRFPPEPNGYLHYGHAKSIFLNFGLAREYGGACHLRFDDTNPTKE
                     ETEYVDSIRDSVKWLGFSWEDPGGNTNEYYASDYFEYLYQFAEELVRRGHAYVDSLSP
                     EQMRALRGTLTEPGKNSPYRERGVEENLKLFREMHLGRFADGEHVLRAKIDMASPNMN
                     MRDPAMYRIRHAAHHRTGDHWCIYPTYDWAHGVSDALENITHSLCTLEFEDHRPLYEW
                     FNERLAEDWTGYDGVKRPGLLQRPLPRQIEFARLNLTCVVLSKRKLIQLVEERHVDGW
                     DDPRMPTLVGARRRGFTPEGFRVFTERIGVAKADSWIDFSVLEDCMREHLNEVAPRRI
                     AVLDPVKLVIENYPPGQEEECEAPNHPQKPEWGRRKLPLSRELWIEREDFSENPPKGY
                     FRLFPGNRVRLRYGYIVECIGYDKTTDTVRCTYDPDTRSGTPGAEKVKVKGNIHWVSA
                     RDSFSGEVRLYDRLFKVTHPGAGDSELVPDLNPDSKKVVTAQLEPALRDARPEERFQF
                     ERHGYFVADHIDSKPGSPRFNRSVTLRDSWNK"
     gene            2659..2820
                     /locus_tag="VEM33_02815"
     CDS             2659..2820
                     /locus_tag="VEM33_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50791.1"
                     /translation="MRNARAANQPSPDIPTSVVRKLTQIACAELKSHKERSTRLRERD
                     ELERRKQSG"
     gene            complement(2894..>3806)
                     /locus_tag="VEM33_02820"
     CDS             complement(2894..>3806)
                     /locus_tag="VEM33_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50792.1"
                     /translation="PHDVIVDREGIVWYSQFDQQFLGRFDPKTLMHAEFAIPVQRPDF
                     PKGTLDLEVDPEGNLWLSHMFQSGIVKFDKKTEKFQAFPLPKGVVNENSQQSMVGPQR
                     WTVDRKVWLNDAGIPGLHRLDMATGKFETWKPYENMKGPHSVYGIYADSKNNIFFMDF
                     GGENVGKIDAATGKLTLFPTPTPRSRPRRGRMDEQDRVWFAEWRAEKIGMFDTRTEKF
                     REWNVPTPHTAPYDVVLDKAGKVWTAGMNTDRVLRMDLESGEFTEYPLPSQTNIRRVF
                     VDNSATPPAFWVGNNHHAAIVKVEPLQ"
BASE COUNT          686 a         1234 c         1187 g          699 t
ORIGIN      
        1 ttgagtccgg gagctagaga tttttctcac actgcgaggt ttaaaatgaa accgcctgac
       61 ttgctgcgtg ccgcatccgt gctcaccctg attttctgcg ccgggcatac ctacggagct
      121 ctgggctctt cctcggctga cccggaacag gcggcggtgt tcatggccat gcaggcctat
      181 ccgttcgaga tcatgggggc aagacgcacg cactggcagt tctaccgcgg gttcagcctc
      241 ctcttcagcg tgaccctgct gctgctcgcc gtgctgctgt ggcaactggc gcggctgtcg
      301 aatcgcgacc cggccggggc gaggccgctt atcgcctcgc tttttctggc ctacctcggg
      361 tttacgatcc tctgcggggt gtatttcttc attgcgcccg cggcgttctc cgcggccgtg
      421 gcgatctgcc tcgcccttgc gttcacctcg gcgagaagcg gctgatcccg ccattcgcag
      481 gcgatggctg ggcgctgggg tagaatcgcg cgattgcccg tgagcgccat gtccgcaccc
      541 tctcccagga agcaggagga agcctcgcac tcgaacttca tccgccagat catcgaagcc
      601 gatctcatgg cgggcaagta cgcgacgcgg aagtggaacg gcaagcccgg ctcggcgctc
      661 tcgcttcacc gggctccgcc cgatccggcg aagatccgca cccgcttccc gccggagccc
      721 aacggctatc tgcactacgg ccacgccaag tcgattttcc tcaacttcgg cctcgcgcgc
      781 gaatacggcg gcgcctgcca cctgcgcttc gacgacacca acccgacgaa agaggagacg
      841 gagtacgtcg actcgatccg cgactcggtg aagtggctcg gcttctcatg ggaggacccg
      901 ggcggcaaca cgaacgagta ctacgcctcg gactacttcg aatacctgta tcagttcgcg
      961 gaagagctgg tgcgccgcgg ccacgcctac gtggacagcc tctccccgga gcagatgcgc
     1021 gcgctgcgcg gcacgctcac cgagcccggc aagaacagcc cctaccgcga gcgcggcgtc
     1081 gaggagaacc tcaagctctt tcgcgaaatg catctgggca ggttcgccga cggcgagcac
     1141 gtgctgcgcg cgaagatcga catggcctcg cccaacatga acatgcgcga cccggccatg
     1201 tatcgcatcc gccacgccgc gcaccaccgc accggcgacc actggtgcat ctaccccacc
     1261 tacgactggg cgcacggcgt gtcggacgca ctcgaaaaca tcacgcactc gctctgtacg
     1321 ctcgagttcg aggaccaccg cccgctctac gaatggttca acgagcgcct cgccgaggac
     1381 tggaccgggt acgacggcgt gaagaggccc ggcctcctgc agcggccgct gccgcggcag
     1441 atcgagttcg cgcggcttaa cttgacctgc gtagtgctct cgaagagaaa gctcatccag
     1501 ctcgtcgagg aaaggcacgt cgacggctgg gacgacccgc gcatgcccac gctcgtcggg
     1561 gcgcgccgcc gcggcttcac ccccgaaggc tttcgagtgt tcacggagcg catcggcgtg
     1621 gcgaaggcgg attcctggat cgacttctcg gttctcgagg actgcatgcg cgagcacctg
     1681 aacgaagtcg ccccgcgtcg catcgcggtg ctcgatccgg tgaagctcgt catcgagaac
     1741 tacccgccgg ggcaggagga agaatgcgag gcgcccaacc acccgcagaa gccggaatgg
     1801 ggaaggcgca agctgccgct ctcgcgcgag ctgtggatcg agcgtgaaga tttctccgag
     1861 aacccgccga agggctactt ccgtctcttc cccggcaaca gggtgcggct gcgctacggc
     1921 tacatcgtcg agtgcatcgg gtacgacaag acgaccgaca ccgtgcgctg cacctacgat
     1981 cccgacacgc ggtcagggac gcccggcgcg gagaaggtca aggtcaaggg caacatccac
     2041 tgggtgagcg cgagggactc gttttcaggg gaagtgcggc tgtacgaccg gctgttcaag
     2101 gtgacgcatc ccggagcggg agacagcgag ctcgtgccgg acctgaatcc ggattcgaag
     2161 aaagtcgtca ccgcccagct cgagccggcg ctcagggacg cacggcccga ggagcggttc
     2221 cagttcgagc gccacggtta tttcgtcgcc gatcacatcg attcgaagcc cggctcacct
     2281 cgattcaacc ggtccgtcac cctgagagat tcgtggaaca agtagcggca tgtccactgc
     2341 accgtcgtcg cctctcgtgg cgatgtcacg cgtctaatgt cgcccggacg cgacacccga
     2401 agccgtgcca gatcagcgtc ctagcatttc ctgctatcgc ggccgtcgct taacggcgac
     2461 ggaatcagga ggcccgcgtg tcgcccacct tgtgctaact atttgatcgt ttgtagattc
     2521 gatggtctgg cacggaggtt gcggtaatcc gctcatcggc gcaggagcag cggcatctgc
     2581 gccttaaaaa acatatgggg gaccgctatg gaccacagcc cgagtgtgac tgccgaagat
     2641 atcgattggt tgcgcaaatt gcgcaacgca agagccgcga accagccttc ccccgacatc
     2701 cccacgagcg tggtgcgcaa gctgacgcag atcgcctgcg cggagctgaa aagccacaaa
     2761 gagcgttcga ccaggttgag agagcgcgac gagctcgagc ggaggaagca gagcggctga
     2821 tctttcgcgc gaaccgcagg gcctatcggg gcgccttgtg cgccccgaat tcgtataccg
     2881 cgtcggcgct tcgctactgc agcggctcga ccttcacgat ggcggcgtgg tggttgttgc
     2941 ctacccagaa cgcgggcggt gtggccgagt tgtccacgaa gacgcgccgg atgttggtct
     3001 ggctcggcag cgggtattcg gtgaattcgc cgctctcgag gtccatgcgc agcacgcggt
     3061 cggtgttcat gcccgcggtc cacaccttgc cggccttgtc gaggacgacg tcgtaaggcg
     3121 cggtatgggg cgtgggaacg ttccattccc tgaatttttc cgtcctggtg tcgaacatcc
     3181 cgattttttc ggcccgccac tccgcgaacc agacgcggtc ctgctcgtcc atcctgcccc
     3241 gccgcggcct ggatcgcggc gtcggggtcg gaaacagagt cagcttcccg gtcgcggcat
     3301 cgatcttgcc gacgttctct ccgccgaaat ccatgaagaa gatgttgttc ttcgagtccg
     3361 cgtagatgcc gtacaccgaa tgcggcccct tcatgttctc gtagggtttc caggtttcga
     3421 acttgccggt cgccatgtcc aggcgatgca gcccgggaat accggcgtcg ttcagccaca
     3481 ccttgcggtc cacggtccag cgctgcgggc cgaccatcga ctgctgggaa ttctcgttga
     3541 cgacgccctt gggcagcggg aacgcctgga acttttcggt cttcttgtcg aatttcacga
     3601 tgccagactg gaacatgtgc gagagccaga ggttgccttc cggatcgact tcgaggtcga
     3661 gcgtgccctt gggaaaatcg ggccgctgca ccggaatcgc gaactcggcg tgcatcagcg
     3721 tcttcggatc gaatctgccg aggaattgtt ggtcgaactg cgagtaccag acgatgcctt
     3781 cccggtcgac gatcacgtcg tgcggc
//
LOCUS       DASPZD010000145         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_425363, whole genome shotgun sequence.
ACCESSION   DASPZD010000145 DASPZD010000000
VERSION     DASPZD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425363"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..936
                     /locus_tag="VEM33_02825"
     CDS             187..936
                     /locus_tag="VEM33_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HYS50793.1"
                     /translation="MELLSDPHAWIAFLTLTALELVLGIDNIIFISILVDKLPGARRE
                     LARRIGLFLAMFMRIGLLFLLAWISGLIEPLFTVFRQEISGRDLILIAGGLFLLWKST
                     KEIHQLLEGEKGADSDAVGATFSGVILQIMIIDIVFSFDSIITAVGMVDHIEVMIAAV
                     IASVLLMMAFAGGIGRFVSARPTVKMLALAFLFVIGVMLIADGFDHHIPKGYVYFAMA
                     FSVCVEMLNQRLRKRPARPVDLRHRYARRDE"
     gene            complement(949..1464)
                     /locus_tag="VEM33_02830"
     CDS             complement(949..1464)
                     /locus_tag="VEM33_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYS50794.1"
                     /translation="MITPQAARMLTRYNSWANKLIFDAVAGLPGDEATKERQSLFKNM
                     VHTLNHNYVIDLIWQAHLEGREHGFAARNTPGHPPLAELWRKQQVVDDWYVAWSERMS
                     EAALGEKLHFTLIGGNKGVMTRAEILLHIVNHTSYHRGFVADLFYQVPGARPPTTDLP
                     VFLREGAAAPV"
     gene            1715..2482
                     /locus_tag="VEM33_02835"
     CDS             1715..2482
                     /locus_tag="VEM33_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HYS50795.1"
                     /translation="MPVLATVAALFLLCWTLAQGTWRVLQPTSPAVARAGDADLTDLR
                     SLGSVLLFGRAAPRSAAAETSVAPSNLNMTLTGVAARATGGCALVIVQGQPEAAFCSG
                     EEISPGVRLDAVERDRIVIVRNGVREAVFMKDAEGGALSVAAPPPPIVQPVGTDRQVV
                     DRRQLQQQLGRPEFLNQALIVPNPDGGFLVRQVQAGSLYEKLGLRPGDVIKNVNGQPL
                     TSMDDVMRLYQQFGTAQRVLVDVQRQGRSETLYYDMR"
     gene            2507..>2807
                     /locus_tag="VEM33_02840"
     CDS             2507..>2807
                     /locus_tag="VEM33_02840"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50796.1"
                     /translation="MRKTPAIAARILAGALAFAAHTGSGQTPDTPSEPPVAPGVPPVA
                     PAAVPAPAAPTSRAPGVTTPRAGEVVLNFQGADLQAVVKAMSQMTGRNMLIDPRVR"
BASE COUNT          442 a          917 c          911 g          537 t
ORIGIN      
        1 cgttgatgaa actcaccgaa cgctattgcg tggtgtatca gactctggca aaacccgcgc
       61 agatttctat ccaggtcgtt tgaaggagtg attcgatgaa aatccgattc gacttcacgc
      121 tcgacgatct cgtggacgcc gcggatcgcg aggccccgaa gcgaggggca tgaactggga
      181 ctctcgatgg aattgttgtc tgaccctcac gcctggatcg cattccttac tctcacggcg
      241 ctcgagctcg tgctcggcat cgacaacatc atcttcattt ccattctcgt ggacaagctg
      301 cccggggcgc gtcgggagct cgcgcgacgg atcggattgt tcctcgccat gttcatgcgc
      361 atcgggctcc tgttcctgct tgcctggatc agcgggttga tcgagccgct cttcacggtc
      421 ttccgccagg aaatttccgg ccgcgacctc atcctgatcg cgggcggcct cttcctgctg
      481 tggaagagca ccaaggaaat ccaccagctg ctcgagggcg agaagggcgc ggactcggac
      541 gccgtaggcg ccaccttctc cggggtgatc ctgcagatca tgatcatcga catcgtgttc
      601 tcgttcgatt cgatcatcac cgcggtcggg atggtcgatc acatcgaagt catgatcgcg
      661 gcggtgatcg cctcggtgct gctcatgatg gccttcgcgg gcgggatcgg gcgattcgtc
      721 tcggcgcgtc ccacggtcaa gatgctggcg ctcgccttcc tcttcgtgat cggggtgatg
      781 ctgatcgcgg acggcttcga ccatcacatt ccgaagggtt acgtgtactt cgcgatggcg
      841 ttctcggtgt gcgtggagat gctcaaccag cgcctgcgca agcggcccgc gcggccggtc
      901 gatctgcgcc atcgctacgc gcgacgagac gagtgacggg cttcgctcct agacgggcgc
      961 cgcggcgcct tccctgagga acaccggcag atcggtcgtc ggcgggcggg cgcctggcac
     1021 ctggtagaag agatcggcga cgaagccgcg gtgatagctc gtgtggttga cgatgtggag
     1081 caggatctcc gcgcgcgtca tgaccccctt gttcccgccg atcagggtga agtgcagctt
     1141 ctcgccgagc gcggcctcgg acatgcgctc gctccaggcg acataccagt cgtccacgac
     1201 ctgctgcttg cgccacagct cggccagcgg cgggtgaccg ggggtgttgc gcgccgcgaa
     1261 cccgtgctcg cgcccctcga ggtgggcttg ccagatgagg tcgatcacgt agttgtggtt
     1321 gagggtgtgg accatgttct tgaacaggct ctgacgctcc ttcgtcgctt cgtccccggg
     1381 cagcccggcg accgcgtcga aaatgagctt gtttgcccag ctgttgtagc gcgtgagcat
     1441 gcgcgcggcc tgcggagtga tcatggtttg cctcgttcgg agctttcttg ccggattatg
     1501 gcaccgccgg cgcccggctt tcggccgccc cctctccgat cggcaggcga cgaaggaggc
     1561 ttcttctcgt gtaagatgaa cggtttatat cgggaactgc aagctttcat caggtgtcta
     1621 caagccccga cccatgacgg catcttcgct ttctgtcgcg cagcgccttg ttcggtcggc
     1681 cggacgcgtc caggccgtgc tctcgcatcg tgcgatgccc gttctagcga cggtcgcggc
     1741 gctgttcctg ctgtgctgga cgctggcgca ggggacctgg cgcgtgctcc agccgacctc
     1801 gcccgcggtg gcgcgcgccg gggacgccga tctcaccgac ctgcgctcgc tcggcagcgt
     1861 gctgcttttc gggcgggccg ccccgaggag cgctgcggcg gaaacgagcg tcgcgcccag
     1921 caacctgaac atgaccctga ccggtgtcgc cgcgcgcgcg accggcggtt gcgcgctcgt
     1981 catcgtgcag ggacaacccg aggcggcctt ctgttcgggc gaagagattt ctcccggcgt
     2041 gcggctggac gcggtggagc gcgatcgcat cgtgatcgtg cgcaacggcg tgcgcgaagc
     2101 ggttttcatg aaggacgcag agggcggcgc tctgagtgtt gccgcgcctc cgcctcctat
     2161 cgttcagccg gtcggcaccg accgtcaagt cgtcgaccgg cggcaattgc aacagcagct
     2221 cggcaggccg gaattcctca accaggcctt gatcgtgccc aaccccgacg gaggcttcct
     2281 ggtgcgccag gtccaggcgg gaagcctgta cgagaagctc gggcttcgcc cgggtgacgt
     2341 gatcaagaac gtcaacggcc agccgctcac cagcatggac gacgtgatgc gtctctacca
     2401 gcaattcggc accgcccagc gcgtgctcgt ggacgtgcag cgccagggcc gcagcgaaac
     2461 gctctactac gatatgcgct gacggcggcg caattccccg acactcatga gaaaaactcc
     2521 tgcaatcgcc gcccgaatcc tcgccggcgc actcgcgttc gccgcgcata ccggttcagg
     2581 gcagacaccc gataccccaa gcgagccgcc cgttgccccg ggcgtacctc ccgtcgcccc
     2641 ggccgcagtg cccgcccccg ccgcgccgac ttcgcgcgca ccaggggtta ctacaccgcg
     2701 agcgggcgag gtggtgctca atttccaggg cgcggacctg caggccgtgg tgaaggcgat
     2761 gtcgcagatg accgggcgca acatgctcat cgacccgcgc gtgagag
//
LOCUS       DASPZD010000146         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1748644, whole genome shotgun sequence.
ACCESSION   DASPZD010000146 DASPZD010000000
VERSION     DASPZD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748644"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1158)
                     /locus_tag="VEM33_02845"
     CDS             complement(22..1158)
                     /locus_tag="VEM33_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYS50797.1"
                     /translation="MRVAVIGAGPAGLLFSLLAKRRYPSWRIEVAEQNPEGATYGFGV
                     VFSDGALTFLERDEPQFSSIMQAAMERWPIQRIVHRDERVDIDGNGFSAIGRLALQEI
                     LRNLCRDAGVRIEHGRAVSALDELAAADVVIGADGSNSLVRAALQAAFEPKIELLSNR
                     FAWYGTTQPFNCLTLTFRSNEDGAFVAHHYRHAPDMSTFVVECDEATWKKAALGRMDD
                     AQSRAYCERVFARDLDGHPLVSNKSLWRQFPLLSNRRWSSGKFVLIGDALRTVHFSIG
                     SGTRLAFEDAIALDRAFGEAGEDVPRALAAFERERRPIVEKLLAAANASSYWYERFRE
                     KMREAPWRLAYDYMTRSGRMTDDRLREQAPKFMRRVEAEREGRS"
     gene            1385..>2238
                     /locus_tag="VEM33_02850"
     CDS             1385..>2238
                     /locus_tag="VEM33_02850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYS50798.1"
                     /translation="MLLIALPMLALTYAIFSDALAPTLPSLPLTFAILPFVIWMALRF
                     GQREVTALNALVCIIAVWHTIEGRGPFASAPLGPSLLLLLAFMGTVVATGLVLNTVVG
                     ERSRAFEALAHALKTLKEETIRDPLTDLYNRRFLRDYLSRELKRATREGIRVAAIMID
                     LDRFKRVNDTAGHAGGDFVLTEIATLLKRHIRGTDVACRYGGEEFTLVLPNATLQSAR
                     HRAEAICAAIREEGVLLMGVTASLGVAIFPDHAADPESLLRAADKALYDAKDRGRNQV
                     RIFARGTA"
BASE COUNT          391 a          759 c          689 g          399 t
ORIGIN      
        1 gcgagtgtag cacgccgcgc atcagcttct gccctctctc tccgcctcga ctcttcgcat
       61 gaatttcggc gcctgctccc gcaagcgatc gtcggtcatg cgcccgcttc tcgtcatgta
      121 gtcgtacgca agccgccagg gcgcctcgcg cattttttcc cggaagcgct cgtaccagta
      181 ggaactcgca ttcgccgcgg cgagcagctt ctccacgata ggcctacgct cgcgctcgaa
      241 cgccgcgagc gcccgcggca cgtcctcgcc cgcctcgccg aacgcacggt cgagggcgat
      301 cgcgtcctcg aacgcgaggc gggttccgga gccaatcgag aaatgcacgg tgcgcagcgc
      361 gtcgccgatc aggacgaact ttcccgagga ccagcgccgg ttcgacagca gcggaaactg
      421 ccgccacagc gacttgttcg aaaccagggg atgcccgtcc agatccctcg caaacacgcg
      481 ctcgcagtac gcgcgcgact gcgcgtcgtc catccggccg agtgccgcct ttttccaggt
      541 ggcctcgtcg cattcgacga cgaaggtgct catgtcgggc gcatggcggt aatggtgagc
      601 gacaaaggca ccgtcctcgt tgctgcggaa ggtgagcgtg aggcaattga acggctgcgt
      661 cgtgccgtac catgcgaagc gattgctgag cagctcgatc ttcggctcga acgccgcttg
      721 cagtgccgca cgcaccaggg aattcgatcc gtcggcgccg atcacgacgt ccgcggcggc
      781 tagctcgtcg agcgcgctca cggcgcggcc atgctcgatc ctcacgccgg catcacggca
      841 caggttccgc aggatctcct gcagcgccag gcgcccgatc gcggaaaaac cgttgccgtc
      901 gatgtccacg cgctcgtcgc gatgcacgat gcgctgaatc ggccagcgtt ccatcgcggc
      961 ctgcatgatg ctcgagaact gcggctcatc gcgctcgaga aacgtgagcg ccccgtcgga
     1021 gaatacgacc ccgaatccgt aggtcgcccc ttcgggattc tgctcggcga cctcgatccg
     1081 ccaggaagga tagcggcgct tggcgagcag cgagaacaac aggccggcgg gtccggcccc
     1141 gattacggcg acgcgcatgt catgactgcg gcatatttca cggtggatcc ccgggtttcg
     1201 cctgccaccg ggtcatagtt cacgaaatac tcccgggggc tggccaagtt gcagtttgaa
     1261 gcttatacta cacggtaaga atcgctcgta atttactgat tctccaagaa agcaaaggtg
     1321 aattttggac gccacagggc gtagacaaca gcgcatttcc gggccgctat ggtacgccgg
     1381 tcgggtgctc ctcatcgccc tgccgatgct cgccctgacc tatgcgatct tcagcgacgc
     1441 gctggctccc accctgcctt cgctgccgct cacgtttgcg atcctgccct tcgtgatctg
     1501 gatggctctg cgcttcgggc agcgcgaggt gaccgcgctg aatgccctgg tatgcatcat
     1561 cgccgtgtgg cacacgatcg agggacgcgg gccgtttgca tcggcgccgc tcggtccttc
     1621 gctcctgctg cttctcgcgt tcatgggcac cgtggtcgcc acgggacttg tgctcaacac
     1681 cgtcgtgggc gaacgcagcc gcgccttcga ggcgctcgcg cacgccttga agacgctcaa
     1741 ggaggagacg atccgggatc ccctgaccga tctctacaac cggcgctttc tccgcgacta
     1801 tttgtcacgc gagctgaagc gcgccacgcg cgaaggcatt cgtgtcgcgg caatcatgat
     1861 cgacttggac cgcttcaaga gggtgaacga cacagcggga cacgccggcg gcgatttcgt
     1921 gctcacggaa atcgcaacgc tgctcaaacg ccatatccgg ggcaccgacg tcgcctgccg
     1981 ttacgggggc gaggagttca cgctggtgct ccccaacgcc actctccaga gcgcgcgcca
     2041 cagagccgag gcgatttgcg cggcgataag ggaggaaggc gtcctactca tgggtgtcac
     2101 cgcgtcgctc ggcgtggcga tcttccccga ccacgccgcc gaccccgagt ccctgctgcg
     2161 cgcggccgac aaggcgctct acgacgcgaa agaccggggg cgcaaccaag tcaggatttt
     2221 cgcgcgcggc accgccaa
//
LOCUS       DASPZD010000147         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_481698, whole genome shotgun sequence.
ACCESSION   DASPZD010000147 DASPZD010000000
VERSION     DASPZD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481698"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..691)
                     /locus_tag="VEM33_02855"
                     /pseudo
     CDS             complement(245..691)
                     /locus_tag="VEM33_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(769..1863)
                     /gene="rodA"
                     /locus_tag="VEM33_02860"
     CDS             complement(769..1863)
                     /gene="rodA"
                     /locus_tag="VEM33_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HYS50799.1"
                     /translation="MIRRLFERLSAKIDGPLFALVTSLLVLGLATLYSAAFQAPGRVG
                     AQALNVLLALVLMWVAAQVPPQVFMRYAVPAFVLVLVMLAGVALFGDVRNNARRWLPL
                     GFTSIQPSELMKIAMPLMLARYFHRHEAALRLREYVIAFALLAVPTAMIARQPDLGTA
                     LLVAAAGFYVIFLAGLSWKVIAGGSAAVLASLPVLWGVLHDYQRRRFLTLIDPSSDPL
                     GAGYHIIQSTIAVGSGGITGKGWTHGTQTHLEFIPERATDFIFAVYSEEFGLIGASLL
                     LVLYLALIMRGLMIAANAPTFFTRLLAGSITLTFFTYAVVNMGMVSGLLPVVGVPLPL
                     ISYGGTSLVTLFLGIGILMSIQHHRALVQT"
     gene            complement(1856..>2624)
                     /locus_tag="VEM33_02865"
     CDS             complement(1856..>2624)
                     /locus_tag="VEM33_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYS50800.1"
                     /translation="FGFGSRTGIDIEGEFVGVLPSPEWKVKRFKNPAQQRWFPGETIS
                     VGIGQGYNSYTPLQLAQAVATLANDGVMYRPHLVDYVENPITGERTYVEPEAYKTIAL
                     KARNLEVVKHAMVGVNKEGTGRGAFAGAGYVSAGKTGTAQVVALSKNEKYSEATVLEH
                     LRDHALFIAFAPAENPRIALAVLVENAGFGARAAAPIARKVLDYYLLGKRVDQPTQPP
                     QPRKRTDKPVEPAPLLGEPTDEPAPPAPAPQENEAND"
BASE COUNT          455 a          816 c          923 g          430 t
ORIGIN      
        1 gagcgaagcc ggctcaatgc gctcccccgg attgcggctt gcgatcgact gtccgctcat
       61 tgcgtcgagg agcagccacg cgcgcgcatc cacgacgggc ggctgcggcg cggcggcgag
      121 cgcgaccccg gaaagcagca gaagaaggca ggcggcgaga cgacgtggca tgggatcgcg
      181 aaggacgaat tataccggcc gcagcgtccc ctcaccgcgc cgcggcactc gagccttgcg
      241 gcaggatggc ttccacttcc acctgaacgc tgccggtgtt cacgaagccg agcttgtacg
      301 ccgccgcgta ggacaggtcg atgatgcgct cggcgtggaa cggcccgcgg tcgttgatgc
      361 gcacgacgac gctgcggccg ttggagatat tggttacgcg cacgtagctc ggaatcggca
      421 ggagagtgtg cgccgcggtc atcgcataca tgtcgtagcg ctcgccgctg gaagtgcgcc
      481 ttccgtggaa cttgcgtccg taccagcttg cgatgccgcg ctgtcggtag ggcctgaggg
      541 actggtaggg aacgtatttc ctgcccaggg cggtgtaggg attgttggcg ccggggttga
      601 ggggctcgga tcgcggtctc gcgtcggcga ttcggtcgag gtcggccgga agcggtccgc
      661 cggggccatc gtcttggtgc ccgtccccga tgtgcttcgc ctggagagtg catccggcga
      721 gagcagtaac cacccacgcc gcgagcagga acgcgggcgc aggccttgtc acgtctggac
      781 gagggcgcgg tggtgctgga tgctcatcaa tatgcctatg cccagaaaga gcgtcacgag
      841 cgaggtgccg ccgtagctga taagcggcag cggcaccccg acgaccggca gcagtccgct
      901 aaccatcccc atgttgacca ctgcgtaggt gaagaaggtg agggtgatcg agcccgcgag
      961 cagccgcgtg aagaaagtcg gcgcgttggc cgcgatcatc agcccgcgca tgatgagcgc
     1021 gaggtagagc acgagcagaa ggctcgcgcc tatcagcccg aactcctcgg aatacacggc
     1081 gaaaatgaaa tcggtggcgc gctcgggaat gaattcgagg tgcgtctggg tgccatgcgt
     1141 ccagcccttg ccggtgatgc cgccggagcc caccgcgatc gtggactgga tgatgtggta
     1201 gcccgcaccg agcgggtccg acgagggatc gatcagcgtg agaaaacgcc tacgctggta
     1261 gtcgtgcagc acgccccaga gcaccggcag gctcgccagg accgccgccg agccgcccgc
     1321 gatcactttc cacgacaggc ctgcgagaaa gatgacgtag aagccggcgg ctgcgacgag
     1381 cagcgccgtg cccagatcgg gctggcgcgc gatcatcgcg gtaggcaccg cgaggagcgc
     1441 gaaggcgatc acgtattcgc gaagccggag cgccgcttcg tgcctgtgaa aataccgggc
     1501 gagcatgagc ggcattgcga tcttcatgag ctccgagggc tggatgctcg tgaatccgag
     1561 tggcagccat cggcgcgcgt tgtttctcac gtctccgaac aaggcgacgc cggcgagcat
     1621 cacgaggaca agcacgaagg cgggaacagc gtagcgcatg aacacctgcg gcggaacttg
     1681 cgcggcgacc cacatcaaca cgagtgccaa gagcacgttg agagcttgcg cgccgacgcg
     1741 tcccggcgcc tggaaggcgg cgctgtagag cgtggcgagg ccgagcacga gcagggaggt
     1801 cacgagggcg aacagcggcc cgtcgatctt ggcgctgagc cgctcgaaga ggcgcctaat
     1861 cattggcttc gttctcttgc ggcgcgggag cggggggcgc cggctcgtcg gtgggctcgc
     1921 cgaggagagg cgcgggttcc accggcttgt cggtgcgctt gcgaggctgg ggcggttgcg
     1981 tcggctggtc gactcgcttg ccgagcagat agtagtcgag caccttgcgc gcgatcggcg
     2041 ccgcggcgcg cgcgccgaac ccggcgttct ccaccagcac ggcgagcgct atccgcggat
     2101 tctccgcggg agcgaatgcg atgaagagcg cgtggtcgcg caaatgctcg agaaccgtcg
     2161 cctcggagta tttttcgttc ttgctgagcg ccacgacctg ggcagtcccc gtcttgccgg
     2221 cgctcacata gcccgcgccg gcgaaggcgc cccgtcccgt gccttccttg ttgaccccga
     2281 ccatggcgtg cttcaccact tccagattcc gggctttgag cgctatggtc ttgtaggcct
     2341 cgggttccac gtaggtgcgc tcgcccgtga tcgggttctc gacatagtcg acgagatgcg
     2401 ggcggtacat cacgccgtcg ttggcgagcg tcgcgacggc ctgcgcgagc tgcagcggcg
     2461 tgtaggagtt gtagccctgc ccgatgccca cgctgatggt ctcgcccggg aaccaccgtt
     2521 gctgcgccgg attcttgaag cgcttcacct tccattcggg agaaggcagc acgcccacga
     2581 actcgccttc gatatcgatt ccggtgcggc ttccaaaccc gaac
//
LOCUS       DASPZD010000148         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1747960, whole genome shotgun sequence.
ACCESSION   DASPZD010000148 DASPZD010000000
VERSION     DASPZD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747960"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VEM33_02870"
     CDS             <1..590
                     /locus_tag="VEM33_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYS50801.1"
                     /translation="RGIACVYDLGSGLLMNLSRWGLSGEPTVPEAVASGADLVVFSGD
                     KLLGGPQAGILVGSEAALAACRADPIARTVRADKLTLAGLEATLALYRDPEVAIREIP
                     VLRMLTESQESIRARAERLGGQLIEGDSEVGGGSFPGAKLRTWLAGFPDESLAERLRA
                     NDPPIIARVAGSRVLLDARTIFPEQVDTVVSALRE"
     gene            583..1137
                     /locus_tag="VEM33_02875"
     CDS             583..1137
                     /locus_tag="VEM33_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HYS50802.1"
                     /translation="MSKRAVFLDRDGTIVDDPGFLHEPDKVKLLPGAAEAIRRLNDAG
                     YRVIVVSNQSGIARGLYSVRDYEMVQERLGALLTAHGARIDAAYFCPHHPQFPGPGGA
                     SCECRKPGLKLFHEAQAAFDIDFSRSWWIGDRLSDVQPALLVGGSAILVATGEGNLHQ
                     GQARALGVMVVADLGEAVQHILSV"
     gene            1200..>2768
                     /locus_tag="VEM33_02880"
     CDS             1200..>2768
                     /locus_tag="VEM33_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYS50803.1"
                     /translation="MRGRILAPLAALAVVASPLMSQKPATRPATTAAGIDIPVARKAV
                     VTIQALDASGRLMASGTGFFVTEGYVITAAHVLEDAAGCRIEMSDGQQQRCSVAAADT
                     AKDVAMLMVSGRPAVTLRWGASESMKDGDDVTVISNPLGQLPGTVSRGIISASRVVNG
                     TKLLQISAAISHGSSGAPVLNARGQVIGIVRSTIEAGQPLNFATATDAIRNLNNDQVA
                     VAEGQALLSSKNMVNTAGSSARASIGSPMSLPQISVGQTITATLSGTDSLYPDTTYYR
                     MYQFTTTPGREITIDLSSSDFDPVLIVRGEGLAQSIVNDDGGPGCASRVSQAFLGPGP
                     YRILVNTTASPHRQTGRFTLAISEGSQPVQGRSENDCSAGGSQVAGSQVVGSGSPATT
                     IRVGETVNGTLTSSDSLYPDNSYFKYYQFTAPAGRAVTIDLSSDEFDPVLIVRGPDLS
                     SSIINDDGGPGCYSRVSRAFPSTGPYRILVNTSSTPERQTGHFSLSITEGTKPVQDGS
                     AATADCQSAENVSGS"
BASE COUNT          465 a          913 c          929 g          461 t
ORIGIN      
        1 gtcgcggcat cgcctgcgtg tacgatttgg gcagcgggct gctgatgaat ctttcaaggt
       61 ggggcttgag cggcgagccc acggttcccg aggctgtggc gagcggcgcg gatctcgtcg
      121 tcttcagcgg cgacaagttg ctcggcggac cccaggccgg cattctcgtc ggcagcgaag
      181 ccgcgctcgc cgcgtgtcgt gccgatccca tcgcacgcac ggtgcgcgcc gacaagctca
      241 cgctggcggg gctcgaggct accctcgcac tctatcgcga cccggaggtc gccattcgtg
      301 aaatcccggt actgaggatg ctcacggaga gccaggaaag catccgcgcg cgtgccgagc
      361 gcctcggcgg acagttgatc gagggagact ctgaggtcgg tggcggctcg ttcccgggtg
      421 ccaaactgcg gacctggctc gccggattcc cggacgaatc gctcgccgag cgcttgcggg
      481 ccaacgaccc gccgatcatc gcgcgcgtcg cgggaagccg ggtgctgctc gacgcgcgca
      541 caatcttccc ggaacaagtg gataccgtag tctcagcact tcgtgagtaa gcgcgcggtc
      601 ttcctcgacc gcgacggcac catcgtcgac gatccgggat ttctgcacga gcccgacaag
      661 gtcaagctgc tgccgggcgc cgccgaggcg attcggcggc tgaatgacgc cggctaccgc
      721 gtcatcgttg ttagtaatca atcgggtatc gcgcgcggtc tctactcggt acgcgattat
      781 gagatggtgc aagaacgcct cggtgcactg ctcacggcgc acggcgcacg tattgacgcc
      841 gcgtacttct gcccgcacca cccgcaattc cccggtcccg gcggcgcctc ctgcgagtgc
      901 cgcaaacccg gcctcaagct cttccacgaa gcgcaggcgg cgttcgacat cgacttttcg
      961 cggtcctggt ggatcggcga ccggctgtcg gacgtgcagc cggcgcttct cgtggggggc
     1021 agcgcgattc tggtcgccac gggggaaggc aatctgcatc aggggcaggc gcgggcgctc
     1081 ggtgtgatgg tcgtcgctga ccttggggaa gcggttcagc acattcttag cgtgtgaatt
     1141 tgtgttcgaa cactcaattc cccagattag cgcacccagc cctactgaag gagtcgcgca
     1201 tgcgcggacg catcctggcc cctctcgccg ccctcgccgt tgttgcctca cccctcatgt
     1261 cccagaagcc ggccacgcgc cccgccacga cagccgcggg aatcgacatc ccggtggcgc
     1321 ggaaggccgt cgtgacgatc caggcgctcg atgcgagcgg ccggctcatg gcgagcggta
     1381 cggggttctt cgtgacggag gggtacgtca tcaccgcggc gcatgtgctc gaggatgccg
     1441 ccggctgccg catcgaaatg agcgacggcc agcagcagcg ctgctcggtg gccgcggcgg
     1501 atacggcgaa ggacgtcgcg atgctgatgg tgtcgggccg tccggcggtg acgctgcgct
     1561 ggggggcgag cgagagcatg aaggacgggg atgacgtgac ggtgatctcg aacccgctgg
     1621 gccagttgcc cggcacggtc tcgcgcggga tcatctccgc gtcgcgcgtc gtcaacggaa
     1681 ccaagctgct ccagatctcg gcggcgattt cgcacggctc cagcggcgcg ccggtgttga
     1741 acgcgcgcgg ccaggtgatc ggcatcgttc gcagcacgat cgaagccggc caaccgctga
     1801 atttcgccac cgccacggac gcgatccgca acctcaacaa cgatcaggtc gcggtggccg
     1861 agggccaggc gctgctcagc tcgaagaaca tggtcaatac ggccggcagt tcggcgcgag
     1921 cgtcgatagg cagtccgatg agcctgccgc agatcagcgt cggacagacg attacggcga
     1981 ccctgtctgg aacggattcg ctgtaccctg acacgacgta ctacaggatg tatcagttca
     2041 cgaccacgcc gggccgcgag attacgatcg atctctcctc gagcgatttc gatccggtgc
     2101 tcatcgtccg cggcgaaggc ctggcgcaat cgatcgtcaa tgacgacggt gggccgggct
     2161 gcgcgtcccg cgtgtctcag gcgtttttgg gaccgggccc gtatcgaatt ctcgtgaaca
     2221 ccaccgcctc accgcaccgc cagaccgggc gcttcacgct cgcgatcagc gagggcagcc
     2281 agcccgtgca aggccgcagc gagaacgact gctcggcagg gggctcgcag gtcgcgggct
     2341 cgcaggttgt gggatcgggg tcgcccgcga cgacgatccg cgtgggcgaa acggtcaacg
     2401 gcacgctgac cagcagcgac tcgctctacc ccgacaacag ctacttcaag tactaccagt
     2461 tcaccgcccc ggcgggacgc gcggtcacga tcgatctctc gtcggacgag ttcgatccgg
     2521 tgttgatcgt gcgtgggccg gacctcagct cctcgatcat caatgacgac ggcgggcccg
     2581 gctgttactc gcgggtgtcg cgcgcattcc cgagcaccgg cccctaccgc atactcgtga
     2641 acacgtcgag cacgcccgag cggcagacgg gtcatttctc cttgtcgatt acggagggca
     2701 cgaagccggt acaggacggc agcgccgcga ccgccgattg ccagagcgcc gagaacgtca
     2761 gcggcagc
//
LOCUS       DASPZD010000149         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1781221, whole genome shotgun sequence.
ACCESSION   DASPZD010000149 DASPZD010000000
VERSION     DASPZD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781221"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VEM33_02885"
     CDS             complement(<1..472)
                     /locus_tag="VEM33_02885"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYS50804.1"
                     /translation="MNAGLEKRAVSRRAAAPGSNSVPPIAVTSGEPAGVGPDVCLALA
                     RKRFAARVVVLGDREMLQSRARQLGLRVRLRDPGSGMTSDSIACLHVPLSYTCRAGAL
                     DRRSAPYVISLLDRAIAGCKAGEFAALTTAPVHKGIINDGGIPFIGHTEYLAERT"
     gene            complement(469..1782)
                     /locus_tag="VEM33_02890"
     CDS             complement(469..1782)
                     /locus_tag="VEM33_02890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYS50805.1"
                     /translation="MRFQLAMLLLTAWGAAFAQGARQEQAVPARVELIDRIVAVVNKE
                     VITQYELAERINRVRKDLERRGTSLPDRGEIERQVLERLIIEKVQLQFARESGVRVDD
                     LELDRTVSRVAEGNKLSLTEFRKTLERDAVNFDGFREELRNEILMNRLRDREVTAKIT
                     VSEGEIENLVLEQGERKDIATEYNIAHILVRVPEQATPEQLEVRRARAEEAVKRLKDG
                     AEFAQVAATYSDAPDSLQGGVIGWRSQQRLPELYVEALTALKPGDISGVFRSPAGFHL
                     LKLLELRGAGAPLLVEQAHVRHILVRTNELVSEDEAKRKLLNLRERIVNGVNFAELAR
                     LNSDDGSASRGGDLGWIYPGDTVTEFERAFTELKQMDVSQPVKTPFGWHLIQVLERRT
                     ADMSSERKRLEARKALLDRKGDEAYQEWLRQLRDRAYVEMRLEER"
BASE COUNT          356 a          673 c          604 g          395 t
ORIGIN      
        1 cggtgcgctc ggcgagatac tccgtgtggc cgatgaaggg gatgccgccg tcgttgatga
       61 tgcccttatg caccggtgcg gtggtcagcg cggcgaactc gccggccttg cagcccgcaa
      121 tggcacggtc gagaagcgaa atcacgtagg gcgcgctgcg ccgatcgagc gcgcccgccc
      181 ggcaggtata tgagagtggc acatgcagac acgcgatcga atccgatgtc atacccgagc
      241 cggggtcgcg cagccgcacg cggagcccga gttgccgcgc acggctctgg agcatctccc
      301 ggtctccaag aaccacgacg cgcgcggcaa atctctttcg cgcgagtgcc agacagacgt
      361 ccgggccgac gcccgccggc tccccggagg tcaccgctat gggcgggacg gaattcgaac
      421 ccggcgcagc ggcgcggcgg gacaccgccc gcttttcaag tccggcgttc accgttcttc
      481 gagccgcatt tccacgtagg cgcgatcgcg caactggcgc agccattcct gataggcctc
      541 gtcgcctttt ctgtccagaa gagccttgcg cgcctcgagg cgcttgcgtt cggaagacat
      601 gtcggccgta cgccgctcca gcacctgaat gagatgccac ccgaacgggg tcttgacggg
      661 ctggcttaca tccatctgct tcaactccgt aaaagcccgc tcgaattccg tcacggtatc
      721 gccgggatag atccatccca gatctccgcc ccgggaagcc gagccgtcgt ccgaattcag
      781 gcgggcgagc tcggcgaagt tcactccgtt tacgatgcgt tcccgcaggt tgaggagctt
      841 ccttttggct tcgtcctcgg aaacgagctc gttggtgcgt accagaatat gacgcacatg
      901 cgcctgttcc acgagcagag gcgctccggc gccgcgcagc tcaaggagct tgagcaggtg
      961 gaatccggcg gggcttcgaa acacaccgct gatgtctccg ggcttcaggg ccgtcaaggc
     1021 ctcgacatac aactccggga gccgctgctg actgcgccag ccgatcacgc ctccctgaag
     1081 cgaatccggc gcatcggagt aggtggcggc gacctgagcg aactcagcgc cgtccttgag
     1141 ccgcttgacc gcctcctcgg cgcgggcacg ccgcacttcc agctgctccg gggtggcctg
     1201 ctcgggtacc cggaccaaaa tgtgcgcgat gttgtactcg gttgcaatgt cttttctttc
     1261 cccctgctcg aggaccagat tctcgatctc accctcgctg acggtgatct tggcggtgac
     1321 ctcgcggtcc cgcaagcgat tcatcaggat ctcattgcgc agctcttccc gaaacccgtc
     1381 gaaattgacc gcatcgcgct cgagcgtctt ccggaattct gtcaatgaga gcttgttccc
     1441 ctcggcgacc cggctgacgg tacgatcgag ctccaaatcg tccacgcgca ccccgctttc
     1501 ccgcgcaaat tgcaactgga ctttttcgat aatgaggcgc tccagcacct ggcgctcgat
     1561 ctcgccgcga tcgggaaggg atgttccgcg gcgctccagg tccttgcgca cgcgattgat
     1621 ccgctccgcc agctcatact gcgtgatcac ctctttgttg actaccgcga cgatccggtc
     1681 gatcagctcg acgcgcgccg gcacggcctg ctcctggcgg gcgccctgcg cgaacgccgc
     1741 cccccacgcc gtcagcagca gcattgcaag ttgaaatctc ataatttcat cgtcgctttc
     1801 gtaactcgat cctcgtccct gacgaggaag caagtcccgc tattttgcag tattgtcgcc
     1861 ggaagtgggc ttccgcgaca ccgattccgg atttcaagcg atcagtcgta gaaatcgaat
     1921 gaatcgccgg ttttcctgtc gagtgagggc gcactgatcc gctggtaacc cgggatgttg
     1981 cgtttcaacg cctcgagggg attggaccca aggcgcgaaa agccgttc
//
LOCUS       DASPZD010000150         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1747266, whole genome shotgun sequence.
ACCESSION   DASPZD010000150 DASPZD010000000
VERSION     DASPZD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747266"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1556
                     /locus_tag="VEM33_02895"
     CDS             30..1556
                     /locus_tag="VEM33_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HYS50806.1"
                     /translation="MTIIRQEDLVQSVADALQYISTYHPRDYIQHLARAYEREQSPAA
                     KDAIAQILSNSRMCAEGRRPVCQDTGIVNVFLKIGMGVRLEGFTGSVADAVNEGVRRA
                     YLHPDNTLRASIVEDPIFGRRNTKDNTPAVIHMEIVPGNTVAVDVAAKGAGSENKSKF
                     AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGVGGTAEKAMMMAKESLMEPIDMHEL
                     LARGPRDAHEELRVELYEKVNRLGIGAQGLGGLSTVLDVKIRTFPTHAGSQPIAMIPN
                     CAATRHLHFMLDGSGPAEFEAPSLEDWPNVTWTPAVQSRRVNLDTLTRAEVAKWKPGE
                     TLLLSGRMLTGRDAAHKRIADMLAQGEKLPVDFSNRVIYYVGPVDPVRDEVVGPAGPT
                     TATRMDKFTEMMLGQTGLIAMIGKGERGPAALEAIRKHKAASLIAVGGAAYLVAKAIR
                     KSRVLAFGDLGMEAIYEFEVKDMPVTVAVDAKGTSVHQTGPAEWEKRIKDFKIPVAVA
                     "
     gene            complement(1599..>2368)
                     /locus_tag="VEM33_02900"
     CDS             complement(1599..>2368)
                     /locus_tag="VEM33_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HYS50807.1"
                     /translation="RLTLGVFPGLDAAESFETLDRYLPFAQYLQAKSGAVVMLIPVRV
                     PGAAIQRMIEDGKSYKLFFGPPVFAAEALKKADFVTLAMEQEKIRGVFLVKARSKLQT
                     VRDVTPSTHVAMPSPKLLLAILALATFAKENIILQPEARRHLDSAESMLLALENGVVD
                     VAVMRDRAGQKIATEKPSEYRIIGQTLDGPGFALIAHRSVSEPLRAKLRQAAIALNND
                     PSPLAVQTRARLRTSPFLPGQDGEFLALQRMMDKRGN"
BASE COUNT          449 a          763 c          780 g          376 t
ORIGIN      
        1 tgccgcatcg ttccagtacg ggattcgaca tgaccatcat cagacaggaa gacttggtcc
       61 agagcgtcgc cgacgcgctg cagtacatca gcacctacca cccgcgcgac tatatccaac
      121 acctcgcgcg cgcctatgag cgcgagcagt caccggcggc gaaagacgcc atcgcgcaga
      181 tcctcagcaa ctcgcggatg tgcgccgaag gccgccggcc ggtctgccag gacacgggca
      241 tcgtcaacgt gttcctgaaa atcggcatgg gcgtgcgcct ggagggtttc acgggttccg
      301 tcgccgacgc ggtgaacgaa ggcgtgcgcc gcgcctacct gcaccctgac aacacgctgc
      361 gcgcctccat cgtcgaggat ccgatcttcg gccgcaggaa cacgaaggac aacactccgg
      421 cggtgatcca catggagatc gtgccgggaa ataccgtcgc ggtggacgta gccgcgaaag
      481 gcgccggttc cgagaacaag tcgaagttcg caatgctgaa cccctcggac tcgatcgtcg
      541 actgggtgct gaagaccgtg cccacgatgg gagcgggctg gtgcccgccg ggcatgctcg
      601 ggatcggggt cggcggcacg gccgagaaag ccatgatgat ggcgaaggag tcgctcatgg
      661 agccgatcga catgcacgag ctgcttgcgc gcggcccgcg cgacgcgcac gaagagctgc
      721 gcgtcgagct ctacgagaag gtgaaccggc tcggcatcgg agcgcaagga ctcggcggcc
      781 tgtccacggt gctcgacgtc aagatcagga ccttcccgac gcatgcgggg tcgcaaccga
      841 tcgcgatgat cccgaactgc gccgcgaccc ggcatctcca cttcatgctc gacggctccg
      901 ggcccgcgga gttcgaagcg ccgagcctgg aggactggcc gaacgtgacc tggactccgg
      961 ccgtgcagtc caggcgcgtg aacctcgaca cgctcacgcg cgcggaagtc gcgaagtgga
     1021 agcccggcga gacgctcctc ctgtcgggaa gaatgctcac cggccgcgac gccgcgcaca
     1081 agcgcatcgc cgacatgctc gctcaaggcg aaaagctccc ggtggacttc agcaatcgcg
     1141 tgatctacta cgtcggcccg gtcgatccgg tgcgcgacga ggtcgtcggc cccgcgggtc
     1201 ccaccacagc aacccgcatg gacaaattca ccgagatgat gctcgggcaa accggcctca
     1261 tcgccatgat cggaaaaggc gagcgcggcc ccgcggcgct cgaggcgatc cgcaagcaca
     1321 aggccgcctc gctgatcgcg gtgggcggcg ccgcctacct cgtcgccaag gcgatccgaa
     1381 aatccagagt gctcgccttc ggcgatctcg gcatggaggc gatctacgag ttcgaggtga
     1441 aggacatgcc ggtcactgtc gccgtcgatg cgaagggcac ttccgtgcac cagaccggcc
     1501 cggccgagtg ggaaaaacgc atcaaggatt tcaagatccc cgtcgcggtc gcgtaggatt
     1561 caaagcgcat gagtgccgcg cacgaccacg agccagcttc agttgccgcg tttgtccatc
     1621 atgcgctgca atgccaggaa ctccccatcc tgcccgggaa ggaagggact ggtcctcagg
     1681 cgcgcgcgcg tctgcacggc gagaggcgac gggtcgttgt tgagggcgat cgcggcctgg
     1741 cgcagcttcg cacgcagcgg ttcggagacg gaccggtgag cgatcagcgc gaatcccggg
     1801 ccatcgagcg tctgtccgat gatgcgatat tcggagggct tctcggtggc gatcttttgc
     1861 cccgcgcggt cgcgcatgac tgcgacatcc acgaccccgt tctcgagggc gagcaacatg
     1921 ctttccgcgg agtcgaggtg acggcgcgcc tccggctgca ggatgatgtt ctccttggcg
     1981 aaagtggcga gggccaggat cgcgagcagc agcttcgggg aaggcatcgc cacatgcgtg
     2041 ctcggtgtca cgtcgcgcac cgtctgcagc ttgctgcgcg ctttcacgag gaagacgccg
     2101 cggatttttt cctgctccat ggcgagcgtg acgaaatcgg ctttcttgag ggcctcggcg
     2161 gcgaagactg gcggaccgaa aaagagcttg tagctctttc cgtcctcgat catccgctgg
     2221 atcgcggcgc cggggactct caccggaatc agcatgacca ccgcgccgct cttggcctgc
     2281 aggtattgag cgaacggcaa gtagcgatcg agtgtttcga acgattcggc ggcgtccaga
     2341 ccgggaaaca cccccagggt caggcgtt
//
LOCUS       DASPZD010000151         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_423826, whole genome shotgun sequence.
ACCESSION   DASPZD010000151 DASPZD010000000
VERSION     DASPZD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423826"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1253
                     /locus_tag="VEM33_02905"
     CDS             72..1253
                     /locus_tag="VEM33_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50808.1"
                     /translation="MRQKTPSTVAIGFAAALLAAAPALAQQKLKIGFITTLSGPQGVV
                     GEYMRNSVELALDHLGRKIAGLDVEVIYGDDQVKPDVGKQVADEMLKKHQVDFVAGII
                     WSNVMLAVAPTVTDAGKFMIGTNAGPHELAGKMCNELFFTTSWQNDDTPEAMGKVMQD
                     QGITDVYVMAPNYAAGKDMVNGFKRSYKGRIVDEVYTRLGQTDYQAELSQLRSKNPKA
                     VFVFYPGGMGISFLRQYSEAGLRGQFPLYSVYTVDEISIPAVKHAALGQYETRYWSPD
                     LKNPANQKYVSDYRKKYGKMPVFYGAQSYDGILLIDSAVRAVKGNLADKKGMIAAMKK
                     ADFASTRGKFSYNTNHFPIQNFYLLKTVTGPAGQDPVMEIQKTVFTNHKDSYYKECPL
                     R"
     gene            1324..2244
                     /locus_tag="VEM33_02910"
     CDS             1324..2244
                     /locus_tag="VEM33_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYS50809.1"
                     /translation="MNWVLALEQTLNGLQLGVMLFLMAAGLTLVFGIMNLVNLAHGSL
                     YMVGAYLATYFYQVTGSYVLGVLLGLAGTLVVGIAVEVLALRTLYEREHLDQVLATFG
                     LILFFNELMAILWGRAAIYTTLPDYLSGHVELFAGLRYPLYRLVIIGVGLAVAGLLWF
                     VIARTRLGMLIRAGASNRAMVAALGVNVRLLYTLVFGFGAALAGLAGLMSGPIHVVQP
                     GMGELILIEVFVVIVIGGIGSIRGALAGALIVGMADTLGRAFVRPMLATVLSTTAADT
                     AGPALASMLIYLLMAIVLALKPQGLFPARS"
     gene            2241..>3178
                     /locus_tag="VEM33_02915"
     CDS             2241..>3178
                     /locus_tag="VEM33_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYS50810.1"
                     /translation="MNSRRRTIVLAAGLVLLAAVPPVAALVNQPFYLDLVRRIMIFAI
                     AALSLNLILGYGGMISFGHAAYLGIGAYAVGVLAHYGINNGYLQWALAMGASALVALA
                     IGAVSIRTSGVYFIMITLAFTQMLYYLGISIAEYGGDDGMRLRVKSQFPGLIDLNDAN
                     AFYYLVLAILVLALFLGQRLVDSRFGMVIRAAKSNEVRTRSIGFSPYPYRLAAFVIAG
                     AVCGLAGALLVNHTAYLTPEFMNWTRSGELMFMVILGGMASIAGPVLGAFALLLVEDA
                     LSGWTQHWQLILGPLLVLSVLFLRRGLAGLLFGRNE"
BASE COUNT          543 a         1012 c         1011 g          612 t
ORIGIN      
        1 tcgcgatcga ggccgcattg gcgtaccagg ggccggttct cgtagaataa agatccaaac
       61 ggagggggat catgagacag aaaacaccaa gcaccgtcgc aatcggtttc gccgccgcgc
      121 tgctcgccgc cgcgccggcg ctcgcgcagc agaagctgaa gatcggtttc atcacgacgc
      181 tctccggccc tcagggagtc gtcggtgaat acatgaggaa ctcggtggag ctggcgctcg
      241 atcatctcgg acgcaagatc gcggggctcg acgtggaggt gatctacggc gacgaccagg
      301 tcaagcccga cgtgggcaag caggtcgccg acgagatgct gaaaaaacac caggtcgact
      361 tcgtcgcggg aatcatctgg tcgaacgtca tgctcgcggt cgcccccacg gtcaccgacg
      421 cgggcaagtt catgatcggc acgaacgccg gcccgcacga gctcgccgga aagatgtgca
      481 acgagctgtt cttcaccact tcctggcaga acgacgacac gcccgaggcg atgggcaagg
      541 tgatgcagga ccagggcatc accgacgtgt acgtcatggc gccgaactac gccgccggca
      601 aggacatggt gaacgggttc aagcgctcct acaagggccg tatcgtcgac gaggtctaca
      661 cgcggctcgg gcagaccgac taccaggcgg agctctccca gctgcgctcc aagaatccga
      721 aagcggtatt cgttttctat cccggcggca tgggcatctc gttcctgcgc cagtactcgg
      781 aagcggggct tcgcgggcag tttccgctct attcggtgta tacggtggac gaaatctcga
      841 tcccggcggt gaagcacgcg gcgctcggcc agtacgagac ccgctactgg agcccggacc
      901 tgaagaatcc ggcgaaccag aaatacgtgt cggactaccg caagaaatac ggcaagatgc
      961 cggtcttcta cggagcgcag agctacgacg gcatcctgct gatcgattcg gcggtgcgcg
     1021 cagtgaaggg caatctcgcc gacaagaaag ggatgatcgc cgcgatgaaa aaggcggact
     1081 tcgcgtccac gcgaggcaag ttcagctaca acaccaatca tttcccgatc cagaatttct
     1141 acctgctgaa gacggtcacc gggccggcag gccaggatcc ggtgatggag atccagaaaa
     1201 cggtcttcac caaccacaag gattcctact acaaggaatg ccccctgaga tagcaggcgg
     1261 ccggaaacgc atcgaggcca cggggcgcgg aacgatccgc gccccgtttt agtttctccc
     1321 gccatgaact gggttctagc tctcgagcag acgctcaacg ggttgcagct cggggtgatg
     1381 ctgttcctga tggcggcggg cctgacgctc gtcttcggca tcatgaacct ggtcaacctc
     1441 gcgcacggct cgctctacat ggtcggcgcc tacctcgcca cctattttta tcaggtcacg
     1501 gggtcgtacg tgctcggcgt gctgctcggt ctcgcgggaa ctctggtcgt cgggatcgcc
     1561 gtcgaggtgc tggccctgcg cacgctctac gagcgcgagc acctcgatca ggttctcgcc
     1621 acttttggcc tgatcctctt cttcaacgag ctgatggcga tcctctgggg ccgggcggcg
     1681 atctacacga ctcttcccga ttacctgagc gggcacgtgg agcttttcgc gggcctgcgc
     1741 tacccgctct accgcctggt gatcatcggc gtcggcctcg cggtggcggg cctgctctgg
     1801 ttcgtaatcg cgcgcacgcg cctggggatg ctgattcgcg ccggggcttc caaccgggcc
     1861 atggtcgcgg cgctcggcgt aaacgtccgc ctgctgtata cgctcgtgtt cggcttcggc
     1921 gctgcgctag cgggcctcgc gggccttatg tcgggaccca tccacgtggt gcagccgggg
     1981 atgggtgagt tgattttgat cgaagtgttt gtagtcatag taattggcgg aatcgggtcg
     2041 atccgcggcg cgctcgcggg cgccttgatc gtcggcatgg ccgatacgct cggccgggcg
     2101 ttcgtccggc cgatgctcgc gacggtgctc tcgacgaccg cggccgacac cgccggcccg
     2161 gcgctcgcct cgatgctgat ttacctcttg atggcgatcg tgctcgcgct caagccgcaa
     2221 ggcctctttc ccgcgcgctc atgaattcac gcaggcgcac gatcgtgctg gcggcgggcc
     2281 tcgtgctcct cgccgcggtg ccgccggtcg cggcgctggt caaccagccc ttctacctcg
     2341 atctcgtccg gcgcatcatg atcttcgcga tcgcggcgct gagcctgaat ctcatcctcg
     2401 gctacggcgg aatgatctcg ttcgggcacg ccgcctacct cggcatcggc gcctacgccg
     2461 tcggggtgct cgcgcactac gggatcaaca acggatatct gcaatgggcg ctcgcgatgg
     2521 gcgcatcggc gctggtggcg ctcgcgatcg gggcggtgtc gatccgcacg agcggcgtct
     2581 atttcatcat gatcacgctc gcgttcacgc agatgctgta ctacctcgga atctcgatcg
     2641 cggaatacgg gggcgacgac ggcatgcgcc tccgggtgaa gagccagttc ccggggctca
     2701 tcgacctcaa cgacgccaac gcgttctact acctcgtgct cgcaattctc gtcctagccc
     2761 tattcctcgg gcagcggctg gtcgattccc gcttcggcat ggtgatccgc gccgcgaagt
     2821 cgaacgaagt gcgcacgcgc tcgatcggct tctcgcctta cccgtaccgg ctcgcggcgt
     2881 tcgtgatcgc gggcgccgtg tgcgggctcg ccggggcgct gctcgtcaac cacaccgcgt
     2941 acctcacccc ggagttcatg aactggacgc gctcgggcga gctcatgttc atggtcatac
     3001 tcgggggcat ggcgagcatc gccgggccgg tgctcggcgc ctttgccctg ctgctcgtcg
     3061 aggatgcgct ttcaggttgg acgcagcact ggcaactgat tctcgggccg ctcctggtac
     3121 tgtcggtgct tttcttgagg cgcggcctcg cgggactgct tttcggcagg aatgaata
//
LOCUS       DASPZD010000152         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_563882, whole genome shotgun sequence.
ACCESSION   DASPZD010000152 DASPZD010000000
VERSION     DASPZD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563882"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEM33_02920"
     CDS             complement(<1..195)
                     /locus_tag="VEM33_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HYS50811.1"
                     /translation="MKSVVILISGRGSNMEAIVGAGLPAKVAAVISNRADAAGLKFAA
                     QSGITFQVVDEREFSSRGAFD"
     gene            278..1420
                     /locus_tag="VEM33_02925"
     CDS             278..1420
                     /locus_tag="VEM33_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS50812.1"
                     /translation="MSIHKCPIRFSAAAAALSWACGAVAQVGVTADKILIGQSAGFTG
                     SVAGAVKEQTAGAKAYFDVVNARGGVHGRKIVLESMDDGFDPKRTPEVIQKLIVEKKV
                     FALFLSRGTPTNEAAIPVLEKYKVPLIGPSTGAMSMYEPPRKYLFPVRASYRSETFKI
                     VDQLVSMGIKKIAVVYTDDSFGKDGLTGVQQAMKEKNIAPVAVASHPRGTIKVEEAVA
                     TLAKAEPQAVIMTVLADAGVALVKQMKKTGQSPLFVTLSNNSSNAFIKNLGEDGPGVG
                     VSQVSPYPFSATIPIVKEFQDAISRSKDVAASYASMEGFIAAKVLVEGLKRAGPKPTR
                     DKLITALESLNRFDLGGVDVTYGPGIRTGTSYIDITIVSKSGKFIR"
     gene            complement(1482..>2839)
                     /locus_tag="VEM33_02930"
     CDS             complement(1482..>2839)
                     /locus_tag="VEM33_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYS50813.1"
                     /translation="FAVLLFQDLSVIPLLALLPLLSDSGAHAAGGWTSAAKALAVIGV
                     VIAASRLVARPVLKTVASYGGREVFTAAALLLVVGAALIMERIGLSPSLGAFLAGVLL
                     ADSEFRHELEADIEPFKGLLLGLFFIAVGMSANLGLVLERPLAVLGLAAGLMIAKFAL
                     MYAIARFGGAPNDTAQRLAVSVSQGGEFAFILFVAAGSLGIFEESTAQLLVVVVTVSM
                     LLALLAFEAHERALARWTERHAAPEFDAISGPGNPVIIAGYGRVGQILSRVLRMCGVP
                     FTALEVSYQQVDFVRRYGSKVYYGDASRLELLEAAKTGEAKLFVLAIDDVEASVKTAA
                     IVRKHFPEVPILARARNRVHLFRLRDLGVKLIWRETFSASLDMARHTLLSLGFGIAAS
                     ERAVSLFKQHDEERLDAQYAVQHDEAQLIQTTKEAAAQLQELFESDGLQPLPGFPRAG
                     A"
BASE COUNT          567 a          916 c          908 g          448 t
ORIGIN      
        1 gtcgaatgcc ccgcgcgagg aaaactcgcg ctcatcgacc acctgaaagg taatgcccga
       61 ctgcgccgcg aatttcaggc ccgcggcgtc ggcacggttg ctgatcacgg cggcgacctt
      121 cgcgggtagc cctgcaccca cgatcgcctc catgttgctg ccgcggccgg agatgaggat
      181 tacgaccgat ttcatggcgc tattctagcg cggcgcacct ttgatcccga ttttgctatt
      241 ctccatctgg ggaccttctt gaaccggggg ctagccgatg tccatccata aatgtccgat
      301 cagattctcc gcggctgctg cagcgctttc ttgggcctgc ggcgccgtcg cccaggtcgg
      361 ggtgacggca gacaagatcc tcatcgggca atcggccggg ttcaccgggt cggtggccgg
      421 agcggtaaag gagcagaccg ccggggcgaa ggcctacttc gacgtcgtca acgccagagg
      481 cggagtgcac ggccgcaaga tcgtgctcga atccatggat gacggcttcg acccgaaaag
      541 aacccccgaa gtcatccaga agctgatcgt ggagaagaag gtgtttgccc tgttcctttc
      601 ccgcggcacg ccgacgaacg aggcggcgat acctgtcctc gagaaataca aggtgccgct
      661 gatcggccct tcgaccgggg ccatgtcgat gtacgagccg ccgcgcaagt atctttttcc
      721 ggtgcgcgcc agctaccgct ccgagacttt caagatcgtc gaccagctgg tcagcatggg
      781 gatcaagaag atcgccgtgg tgtacaccga cgattcattc ggcaaggacg gcctcaccgg
      841 ggtgcagcaa gccatgaaag aaaaaaatat cgcgccggtt gccgtcgcga gccacccccg
      901 ggggaccata aaagtcgagg aggcggtggc caccctcgca aaggccgaac cgcaggcggt
      961 gatcatgacc gttctggccg atgcaggcgt cgcactcgtc aagcagatga agaagaccgg
     1021 ccaaagcccg ctgttcgtga ccctctcgaa caacagctcg aacgccttca tcaagaacct
     1081 cggggaagac gggcctggag tcggcgtgag tcaggtgtcg ccgtacccgt tttcggcgac
     1141 cataccgatc gtcaaggaat ttcaggacgc catcagccgc agcaaggacg tggccgcttc
     1201 gtatgcctcc atggagggat tcatcgccgc caaggttctg gtcgagggct tgaagcgcgc
     1261 cggcccgaag ccgacgcggg ataagctgat cacggctctg gaatccctga atcgtttcga
     1321 cttgggtggc gtcgacgtca cctatgggcc gggaatcagg acgggcacga gttatatcga
     1381 catcaccatc gtcagcaaga gcggcaaatt catacgctag cgggcgaggc aagtcacagg
     1441 atggcgcgct gccgcgcgtc gcgggcgcgg acacccgcgt atcaagctcc ggcgcgcgga
     1501 aatcccggca gcggctgcaa gccgtccgat tcgaacagct cctgcaactg cgccgcggct
     1561 tccttggtgg tctggatgag ctgcgcctcg tcgtgctgga ccgcgtactg cgcatcgagg
     1621 cgctcctcgt cgtgctgctt gaacaggctc acggcgcgct ccgaggcggc gattccgaaa
     1681 cccagggaaa gcagcgtgtg ccgcgccatg tccaggctcg ccgagaaggt ttcgcgccag
     1741 atgagcttga cgccgaggtc gcgcagccgg aacaggtgaa cgcggttgcg cgcgcgggcg
     1801 agaatcggaa cctcaggaaa atgcttgcga acgatcgcgg cagtcttcac cgaggcctcg
     1861 acgtcgtcga tcgccagcac aaagagcttg gcttcgcccg tttttgccgc ctcgagcaac
     1921 tccaggcgcg aagcgtcgcc gtagtacacc ttgctgccgt agcgccgcac gaaatccact
     1981 tgctggtagc tcacttcgag cgccgtgaac ggcacgccgc acatgcgcag cacgcgcgaa
     2041 aggatctgcc ctacacgccc gtaaccggcg atgatgaccg gatttccggg cccggagatc
     2101 gcatcgaact ccggcgccgc gtgccgctcg gtccagcgcg cgagagcgcg ctcgtgcgct
     2161 tcgaatgcga gcagcgcgag cagcatcgag acggtcacga ccaccacgag gagctgcgcg
     2221 gtgctctcct cgaatatgcc gaggcttccg gcagcgacga acaggatgaa ggcgaactcg
     2281 ccgccctgag agaccgaaac cgcgagccgc tgcgcggtgt cgttcggcgc gccgccgaac
     2341 ctggcgatcg catacatcaa ggcgaacttc gcgatcatga ggccggctgc gaggccgagc
     2401 accgcgagcg gccgttccag aacgagaccg aggttcgcgc tcatccccac cgcgatgaag
     2461 aaaagaccga gcagcaggcc cttgaagggc tcgatgtcgg cctccagctc gtggcggaac
     2521 tcggaatcgg cgaggagcac cccggcgagg aaagcgccga gcgagggcga caggccgatt
     2581 ctctccatga tcagcgcggc gcccaccacg agcagcagtg ccgccgcggt aaatacttcg
     2641 cggccgccgt agctcgccac cgtcttcagc accgggcgcg ccacgaggcg gcttgccgcg
     2701 atgaccaccc cgatgaccgc caaggccttc gccgcggaag tccacccgcc agcggcgtgc
     2761 gctccggaat cgctcaggag cggcagcagc gcgagcagag ggatcaccga aagatcctgg
     2821 aacaagagca ccgcgaacg
//
LOCUS       DASPZD010000153         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_752576, whole genome shotgun sequence.
ACCESSION   DASPZD010000153 DASPZD010000000
VERSION     DASPZD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752576"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..208
                     /locus_tag="VEM33_02935"
                     /pseudo
     CDS             1..208
                     /locus_tag="VEM33_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
     gene            233..601
                     /locus_tag="VEM33_02940"
                     /pseudo
     CDS             233..601
                     /locus_tag="VEM33_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            917..1099
                     /locus_tag="VEM33_02945"
                     /pseudo
     CDS             917..1099
                     /locus_tag="VEM33_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1152..>2204
                     /gene="trkA"
                     /locus_tag="VEM33_02950"
     CDS             1152..>2204
                     /gene="trkA"
                     /locus_tag="VEM33_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HYS50814.1"
                     /translation="MRIVILGAGQVGSSVAESLASEANDITVVDIDGTRLRNLRDRLD
                     LRTVLGSAAHPSVLAEAGIEDCDMMIAVTQSDETNLVASKLAQVLFNVPTRIVRLRAT
                     DYLSNERVLGPDCFDVDLAICPEQILTDYIVKLIEFPEALQVLEFAHGKASLVAVRAF
                     QGGPLVGHPLKDLRKHIPSVDTRIAAIFRGDSPILPEGDTVVQAGDEVFCLAATEDIR
                     QVMHELRRMDAPVRRVMIAGGGNIGLRLARAIERDYAIKIIEYDRRRCEILSTRLDRA
                     LVLQGDVTDEGLLAAENVSDMDLFVAVTNDDENNIMSCLLAKRMGARRVVALINRRSY
                     VDLLQAGEIDIAISPAQ"
BASE COUNT          397 a          717 c          710 g          380 t
ORIGIN      
        1 gctgctcgat atctggatgc ccgacgcgga cggcatcacc cttttgaagg agtgggccac
       61 cggaggccag ctcaccatgc cggtgatcat gatgtcgggc cacggcacca tcgataccgc
      121 ggtggaggcc acgcgcatcg gcgcgatgga tttcctggag aagccgatcg cgttgcagaa
      181 gctgctcgcg acggtgaagc gcgcgctgcg tgcgggcgag tcacgtgcgg cgatgccgct
      241 cagcatcgcg aatttcggcc gctccctggc gatcaccgag ttgaagaagc gcctggcgag
      301 gatttccgcc gcgggcgcgg cggtgctcat gcgcggcgag aagggggtga tgccggaact
      361 ctacgcgcgt tacctgcagc agcccaacgc gccctggatc gatgcggcgc acgcgctcaa
      421 cgaggcgtcc caggatctgc tgcagcaggt atccagcggc gtgttgttcg tcggggaact
      481 tgcactgctg tccaaaaacc agcagaagca cctcgcgttt ctcgcggccc gcgcggaaaa
      541 gagcaacgtg cggctggtgt cctttaccgc ggaggagccg aagcacctgg tggaatcgct
      601 gggtttcgac gcggccttaa tgcaggcgct cgccggaatc gtcattgcgc ttccggctct
      661 gcgcgaacat ccggaggata tcccggagat cgcgaccctc atgcttgccc agctggtgga
      721 aacgcgtttc tgtccgccgc gaaccttttc caccggggcc ctcaacgtcc tgcgctattt
      781 cacctggcct ggcaacctcg aggatctcgc ggcggcagtg cgcaatctgg ccctcaccag
      841 cctggaggag gaaatcggcg tcgccgacgc ggagcgcgtg ctgccgcagt tcgtgcgccg
      901 cgccgcggag gccgatctgt cgctcgatct cccgctgcgc gaggcccgcg aggccttcga
      961 gcgaacctac ttcgagcatc atctcgcgca ggtgaacggc tccatcgcgc gcgtcgccga
     1021 gcgaagcggt ctggaacgca cgcacttgta ccgcaagttg aaagcgctcg gcattctcgc
     1081 cgggcgaaaa gaagactgac tccggtacga cttcggcata aaatgcgtgc tttttcgcac
     1141 gggctcccgc cttgagaatc gtaatcctcg gtgccggaca ggtcggctca tcggttgccg
     1201 aaagcctggc gtccgaagcc aacgacatca cggtcgtgga catcgacgga accaggctgc
     1261 gcaacctccg cgaccgcctg gatctgcgca cggtgctcgg cagcgccgcg cacccctccg
     1321 tgctggctga ggcgggcatc gaggactgcg acatgatgat cgcggtaacg caaagcgacg
     1381 agaccaacct ggtcgcctcc aagctggcac aggtgttgtt caacgtcccg acccgcatcg
     1441 tccgcctgcg cgccaccgac tatctttcca acgagcgcgt gctcggcccg gactgcttcg
     1501 acgtggatct ggcaatctgt ccggagcaga tcctcaccga ctacatcgtc aagctcatcg
     1561 agtttcccga agcgctgcag gtgctggagt tcgcgcacgg caaggcgagc ctggtcgcag
     1621 tgcgcgcctt ccagggcggt cccctggtcg gtcacccgct gaaggacttg cggaaacaca
     1681 ttccgtccgt ggatacgcgc attgccgcga tattccgcgg cgacagcccg atcctgcccg
     1741 agggcgacac ggtggtgcag gccggagacg aagtattttg cctcgccgcc accgaggaca
     1801 tccgccaggt catgcacgag ttgaggcgca tggacgcgcc ggtgaggaga gtcatgatcg
     1861 ccgggggcgg caacatcgga ttgcgattgg cgcgggcgat cgagcgcgac tacgcgatca
     1921 agatcatcga atacgacagg aggcgctgcg agatactctc cacccgcctc gaccgggcgc
     1981 tcgtattgca gggggacgtc accgacgaag gactcctggc ggccgagaac gtctccgaca
     2041 tggacctctt cgtcgcggtg accaacgacg acgaaaacaa catcatgtcg tgcctgcttg
     2101 ccaagcgcat gggtgcgcgc cgcgtcgtgg cgctcatcaa ccgccgctcc tacgtcgatt
     2161 tgctgcaggc cggcgaaatc gatatcgcga tttccccggc gcag
//
LOCUS       DASPZD010000154         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1791321, whole genome shotgun sequence.
ACCESSION   DASPZD010000154 DASPZD010000000
VERSION     DASPZD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791321"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VEM33_02955"
     CDS             complement(<1..398)
                     /locus_tag="VEM33_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HYS50815.1"
                     /translation="MRVVVIGGYGNFGARVCRALCGSPDMQVVAAGRHPDAGRDGLAD
                     LNLQHARLDHSARDFPAALARLAPELVIHCAGPFQSQDYRVALAALAAGAHYLDLADG
                     RQFVTRFPYYVHPAAREAQRVAISGASSVPA"
     gene            572..1732
                     /locus_tag="VEM33_02960"
     CDS             572..1732
                     /locus_tag="VEM33_02960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HYS50816.1"
                     /translation="MPRALARVLALDDFEEPARRYLPRPMFGYVSGGAETNASLRANR
                     AQYDEIELSPRVLVDVSGRTAKTALFGRAYGAPFGIAPMGGTSMAAFRGDVVLARAAA
                     AAGVPMIMSGASLTRLEDVRSAGATAWFQAYLPGETGPITQLVERVGRAGFDTLVLTV
                     DVPVSANRENNVRSGFSRPLRPTLRLAWDSLLRPRWLLGMFLRTLLVDGMPHFENMGA
                     RIPLITRASRGDGERDRLAWTHLELMRKLWKGRLVLKGVLAPEDARIARESGVDGVIV
                     SNHGGRQLDGALAPLRALPAVVAQAGGMTVMMDGGIRRGTDVLKALALGAQFVFVGRP
                     FLYAAAIGAEAGVRHGIGLLSDEIERDMALLGITTLAQMTRERVRPRTAGFA"
     gene            1753..>2237
                     /locus_tag="VEM33_02965"
     CDS             1753..>2237
                     /locus_tag="VEM33_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HYS50817.1"
                     /translation="MKTCSSLTLAIAILASGLAPARAATLGGPLTLEDEGAFFVNGKT
                     VTSNFPGASLVTGPSAPGRITVNQMYVHYRIPSGKHGAPVVMVHGSNHTGMTYETTPD
                     GREGWATYFVRKGAPVYVVDHSGRGRSGFDPTPFNQVRDSGGANPASLPTLLLATHER
                     A"
BASE COUNT          333 a          808 c          787 g          309 t
ORIGIN      
        1 gcgggcacgc tgctcgcgcc cgagatggcg acgcgctgcg cctcgcgcgc ggccggatgg
       61 acgtagtacg gaaagcgcgt cacgaattgc cggccgtcgg cgaggtcgag gtagtgcgcg
      121 ccggccgcca gcgcggcgag cgcgacgcgg tagtcctgcg actggaacgg cccggcgcaa
      181 tggatcacga gctccggggc gaggcgcgcg agcgccgcgg ggaaatcgcg cgccgagtga
      241 tccagccgcg cgtgctgcag attcaggtcc gcgaggccgt cccgccccgc gtccggatgg
      301 cggccggcgg cgacgacctg catgtccggc gagccgcaaa gcgcgcggca gacgcgggcg
      361 ccgaaattgc cgtacccgcc gatcacgacg acgcgcatgg atgttccagt cgagagctgg
      421 acgggattct aaccggcgaa gcaaccgggg gcgaacccct ctacaatcgt cgggtttccc
      481 tcgcctgaac gagcccatgc ccgacaccag cgaaacaagc caacccgtct ccgcaacgcg
      541 ctccgatgcc gagtggcgcg tcgaccgcgc gctgccgcgc gccctcgcgc gcgtcctcgc
      601 gctcgacgat ttcgaggagc cggcgcgccg ctacctgccg aggcccatgt tcggctacgt
      661 ctcgggcggc gcggagacga acgcgtcgct ccgcgcgaac cgcgcccagt acgacgagat
      721 cgagctcagc ccgcgcgtgc tggtggacgt gtccgggcgc acggcaaaga cggcgctttt
      781 cggccgcgcc tacggcgcgc ccttcggcat cgcccccatg ggcggcacct cgatggcggc
      841 gttccggggc gacgtggtgc tcgcgcgcgc ggcggcggcc gccggcgtcc cgatgatcat
      901 gagcggcgcg tcgctcacgc gcctggaaga cgtgcgctcg gccggcgcca ccgcctggtt
      961 ccaggcctac ctgccggggg aaacagggcc gatcacgcag ctggtcgagc gcgtcggacg
     1021 cgcaggcttc gatacgctcg tcctcaccgt ggatgtcccg gtgagcgcga accgcgagaa
     1081 caacgtaagg agcggcttca gccggccgct gcggccgact ctgcgcctcg cctgggacag
     1141 cctgctccgc ccgcgctggc ttctcgggat gttcctgcgc acgctcctcg tggacggcat
     1201 gccgcatttc gagaacatgg gcgcgcgcat cccgctcatc acccgcgcct cccgcgggga
     1261 cggggagcgc gaccggctcg cgtggaccca cctggagctg atgcgcaagc tatggaaagg
     1321 acgcctggtc ctgaaagggg tgctcgcccc cgaggacgcg cgcatcgccc gcgagagcgg
     1381 ggtggacggc gtcatcgtgt cgaaccacgg cgggcggcag ctggacgggg ctctggcgcc
     1441 tttgcgcgcg ctccccgcgg tggtcgcgca ggcgggcggg atgaccgtga tgatggacgg
     1501 cggcatccgc cgcggcaccg atgtcctgaa ggcgctcgcg ctcggcgcgc agttcgtatt
     1561 cgtcggccgg cccttcctct acgcagccgc gatcggcgcg gaagccggcg tgcgccacgg
     1621 gatcgggctc ctctccgacg agatcgagcg cgatatggcg ctccttggaa tcaccacgct
     1681 tgcgcagatg acgcgggaac gggtcaggcc gcgaaccgct ggattcgcat gaacgcccga
     1741 agggagtcga cgatgaaaac gtgctcgagc ttgaccctcg cgatcgcgat cctcgcctca
     1801 ggccttgcgc cggcgcgcgc cgcgacgctg ggcggcccct tgaccctcga ggacgaaggc
     1861 gcctttttcg tcaacggcaa gaccgtcacg tcgaattttc cgggcgcgtc gctcgtcacc
     1921 ggtccctcgg cgccggggcg catcaccgtc aaccagatgt acgtgcacta ccgcatccct
     1981 tcgggcaagc acggcgcgcc cgtggtgatg gtgcacgggt cgaatcacac cggaatgacc
     2041 tacgagacca cgcccgacgg gcgcgaaggc tgggcgacgt acttcgtgcg caagggcgca
     2101 cccgtatacg tcgtcgatca ctcgggacgc ggccgctcgg gcttcgaccc gacgccgttc
     2161 aaccaggtac gcgactccgg cggcgccaac cccgcctcgc tccccactct gctcctcgcc
     2221 acccacgagc gcgcgtg
//
LOCUS       DASPZD010000155         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1714370, whole genome shotgun sequence.
ACCESSION   DASPZD010000155 DASPZD010000000
VERSION     DASPZD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714370"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VEM33_02970"
     CDS             complement(<1..950)
                     /locus_tag="VEM33_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS50818.1"
                     /translation="MKILCALLALASLASPVAAEEARSALAGLAAYEGVDRHQRLLEG
                     ARREGFLSLYTSFPPEDVATLNAAFEKKYGVKVRTWRAASEKVLQRTVAEAKAGRDEV
                     DLVDSNSVPLELLRREGLLQAVRSPYHADLLPAAVPRHRTWVWARLSVFVQAYNTRLV
                     KKADLPASYGDLLDPRWKGRLGIEASDEDWFAEVVRNLGEEKGLRLFRDMAAKNGLSV
                     RRGHSLLAQMVASGEVPFALTVYNFTAEQLKGQGAPLEWFMLSPAIAHGNGFAVVRRA
                     PHPHAALLYYDFMIGDDGQRILAERKSVPTSRKIPTAIDR"
     gene            complement(947..2230)
                     /locus_tag="VEM33_02975"
     CDS             complement(947..2230)
                     /locus_tag="VEM33_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYS50819.1"
                     /translation="MFSVGRNELLTRVGRGTPIGELFRRYWLPVLLAEELPEPDCAPV
                     RVKLLGERLIAFRDTQGRIGLIEEFCAHRGVSLWFGRNEENGIRCPYHGWKYDVTGQC
                     VDLPSEPEESGMRKNIRLNSYQAIERSGVVWAYLGPAEEKPEEPAYEWTRVPPAHRYL
                     SKRLQETNYLQAMEGALDSIHSGFLHRYSVADDPLLRRDPESLALLLADRKPKIVPEV
                     SPAGLYVATRRNAGEDRYYWRVTQWLMPCFSFFPPYGDNPCGGHAFVPIDDEHCWTFS
                     IDYHPRRALTGAEREAAHAGRGIHVRLIEGSSVPVANKRNDYLIDRAAQKAKKTFSGV
                     FDVGVQDAAIQESMGTIQDRSREHLVSSDNGIVKTRKRLMDAAKAVERGLAPPGLDPA
                     VQRARAVSMVVPRGLSLSDAVAMAQKDPPKTVPAA"
BASE COUNT          366 a          786 c          765 g          409 t
ORIGIN      
        1 tcccgatcga tggcggtggg gatcttccgg ctcgttggaa cggatttgcg ctcggccagg
       61 atccgctggc cgtcgtcgcc gatcatgaag tcgtagtaca gcagggccgc atgcgggtgc
      121 ggcgcgcgcc tcaccacggc gaacccgttg ccgtgcgcga tcgcgggcga gagcatgaac
      181 cactccagcg gcgcgccttg tcctttgagc tgctcggcgg tgaaattgta gacggtgagc
      241 gcgaagggca cttcgccgga ggcgaccatc tgtgcaagca ggctgtgccc tcttcgcacc
      301 gacaggccgt tcttcgccgc catgtcgcga aacagcctca agcctttttc ttccccgagg
      361 ttccgcacca cttccgcaaa ccaatcctcg tccgaggcct cgatgcccag cctccctttc
      421 cagcgcggat cgaggaggtc gccgtaagac gcgggcagat cggctttctt caccagccgc
      481 gtgttgtagg cctggacgaa tacgctcaac cgggcccaca cccacgtgcg atgccgaggt
      541 accgcggccg ggagcagatc ggcgtggtag ggcgagcgca ccgcctgcag cagcccctcg
      601 cggcgcagca gctcgagcgg caccgagttg ctgtccacca gatcgacttc gtcgcgcccc
      661 gccttcgctt ctgccacggt gcgctgcaag accttctcgg aggcggcgcg ccaggtgcgg
      721 actttcacgc cgtatttctt ttcgaatgcc gcgttcagcg tcgcgacgtc ctcgggggga
      781 aacgacgtat acaggctgag gaagccctcg cggcgcgcgc cttccagcag gcgctgatga
      841 cggtcgacgc cctcgtatgc ggccagtccc gcgagcgcgc tcctcgcctc ctcggccgcg
      901 acgggcgaag cgagactcgc gagcgcgagc aatgcgcaca ggattttcac gccgccggca
      961 ccgttttcgg cgggtctttc tgcgccattg cgaccgcgtc ggacaagctc agcccgcgcg
     1021 gcaccaccat ggacaccgcg cgggcgcgct gcacggccgg atcgaggccc gggggcgcga
     1081 gcccgcgctc gaccgccttt gcagcgtcca tcagccgctt gcgcgtcttg acgatgccgt
     1141 tgtccgagga gacgagatgc tcgcggctgc ggtcctggat cgttcccatg ctctcctgta
     1201 tggcggcatc ctgtacgccg acgtcgaaca cgccggaaaa agtcttcttc gccttctgcg
     1261 ccgcgcggtc gatcaggtag tcgttgcgct tgttcgccac cgggacggag ctgccctcga
     1321 tgaggcgcac gtgtatgccg cgtccggcgt gggcggcttc gcgctcggcg ccggtgagcg
     1381 cccggcgcgg gtggtagtcg atgctgaaag tccagcagtg ctcgtcgtcg atcggcacga
     1441 acgcgtggcc gccgcagggg ttgtcgccgt aaggcggaaa gaagctgaag cagggcatca
     1501 gccactgcgt gacgcgccag tagtagcggt cttcccccgc gttgcgccgg gtcgccacgt
     1561 acagtcccgc aggagaaacc tcgggaacga ttttcggctt gcggtcggcg agcagcagcg
     1621 cgaggctttc cggatcgcgc ctgagcaagg ggtcatcggc gaccgagtag cggtgcagga
     1681 atccggaatg aatcgaatcg agcgcgcctt ccatcgcctg caggtaattc gtttcctgca
     1741 ggcgcttgga caggtatcgg tgagcgggcg gcacccgggt ccattcgtac gccggctcct
     1801 ccggcttctc ttccgcgggg ccgagataag cccagaccac cccggagcgt tcgatcgcct
     1861 gatacgaatt gaggcggatg ttttttcgca tgccggactc ctccggctcc gaaggcaggt
     1921 ccacgcactg gccggtcacg tcgtatttcc acccgtgata ggggcagcgg attccatttt
     1981 cctcgttgcg cccgaaccac aggctcactc cccggtgagc gcagaactcc tcgatcagcc
     2041 cgatcctgcc ttgcgtgtcg cgaaacgcga tcaggcgctc cccgagcagc ttcacgcgca
     2101 ccggcgcgca atccggctcg ggcagctctt ctgcgagcag caccggcagc cagtagcgcc
     2161 ggaagagctc ccctatggga gttccccggc cgacgcgggt cagcaactcg ttgcgtccga
     2221 cggagaacat cggctattgc gacgccgtgg gggtggagac cgcgccgcga ccgtagaggg
     2281 tgtcctcgaa gatccgggtc cagcggtcat gatcggcgag caccgc
//
LOCUS       DASPZD010000156         4075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_783125, whole genome shotgun sequence.
ACCESSION   DASPZD010000156 DASPZD010000000
VERSION     DASPZD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783125"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VEM33_02980"
     CDS             complement(<1..353)
                     /locus_tag="VEM33_02980"
                     /inference="COORDINATES: protein motif:HMM:NF026164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50820.1"
                     /translation="MFDRAKFWRYTRYTVLTVALLAAGFTLYLDFRVRSEFEGRRFAL
                     PARIYARPLELHAGLRIAQIDVVRELQETGYGQGPREGDPGWFANDNDWLEVAVRPFV
                     FWDGPQPAKRVRVAF"
     gene            complement(483..740)
                     /locus_tag="VEM33_02985"
     CDS             complement(483..740)
                     /locus_tag="VEM33_02985"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HYS50821.1"
                     /translation="MVVEHFRNGDAVPVYRRFRERGRMAPEGLNYVSSWVDDKMATCY
                     QVMETAGRALLDEWMRHWEDLVDFDVHPVMTSQEAAQRVAL"
     gene            complement(1391..1924)
                     /locus_tag="VEM33_02990"
     CDS             complement(1391..1924)
                     /locus_tag="VEM33_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50822.1"
                     /translation="MSHLFETATSGRSKCRGCAQAIQRGDLRFGERLPNPFAEGEMTV
                     WLHPHCAAYKRPEPLLQALVETPANVPDRESIERAARSSLAHRRLPRIDGAERSPGAQ
                     AKCRSCREPIARGSWRIRLVFYEEGRFVPGGFVHLDCRKAYFETDDILDRVLQFSRDL
                     SADEREELGRACGEEKR"
     gene            complement(1944..3626)
                     /locus_tag="VEM33_02995"
     CDS             complement(1944..3626)
                     /locus_tag="VEM33_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYS50823.1"
                     /translation="MAEPYQSPTLAARRAQMFPVLAGEEIARLRRFGEPRRYADGECL
                     FETGKTSPGMFVVLSGTLRITGRDGHGHDLLIAELSAGGFSGEIGQLSGRRALADGVA
                     VGAVEALLVAPELLRALLIAEADLGEEIMRALILRRVGLIEMGAGGPVLIGAPSSPDV
                     ARLRSFLTRNGIPHLVFDPASDPDAHGFIERYAARPGDMPLVAFPDGSVLRNPGENEL
                     ARCIGMLDAADAERVHDVAVVGAGPAGLATAVYAASEGLSVLVIDARAFGGQAGASAR
                     IENYLGFPTGISGQALAGRAYTQAQKFGARMLIPAEAVRLDCGRPEERDPLALELAGT
                     RGVRSRTVVVATGARYRRPDCADLKALEGRGVWYWASPIEAKACAGQEVALVGGGNSA
                     GQAAVFLAGHVAKVWMLVRGPGLAASMSRYLIDRIAAIPNIELRTRTEIVALEGSRES
                     GVESLRWRHRDSGAEESRAIRNVFLFLGADPATEWLKDCDVAFDDKGFVRTGAGGRLP
                     LETSVPGVFAIGDVRSGSVKRVGGAIGEGAAVVAQIHAFLARENAEVEARAA"
     gene            3738..>4075
                     /locus_tag="VEM33_03000"
     CDS             3738..>4075
                     /locus_tag="VEM33_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYS50824.1"
                     /translation="MRFPRPVVVLLALLHIYIGLRLLLPFDAVIQLVGGALLAVCFWL
                     LPKGFRIRDDRGAWGVMLPWLTMGFFSWLLVLTVFRDVSLLVSAFALSAQAHESWVRI
                     SAIGVMALTPA"
BASE COUNT          662 a         1423 c         1322 g          668 t
ORIGIN      
        1 tcgaaggcga cgcgcacgcg cttcgcgggc tggggcccgt cccagaacac gaacggccgc
       61 accgccacct cgagccagtc gttgtcgttc gcgaaccagc cggggtcgcc ctcgcgcggg
      121 ccctggccgt agcccgtctc ctgcaactcg cgcaccacgt cgatctgcgc gatgcgcagg
      181 cccgcgtgca actccagcgg ccgcgcgtaa atccgcgccg gcagcgcgaa gcggcggccc
      241 tcgaattcgc tgcgcacgcg gaaatcgaga tacagcgtga acccggccgc gagcaaagcg
      301 accgtgagca ccgtgtagcg ggtgtagcgc cagaatttag cgcgatcgaa catcggcgga
      361 ttttacttcg ttgcggggca gagtcgagag accgaggaga gttgaagggc gaaaatgcgc
      421 gggcggccat gctcaggtgt cgccttgagg cgacaccgat ttcacagacg ctcagcttct
      481 gtctaaagtg ccacccgctg tgccgcctcc tgggatgtca tgacgggatg gacgtcgaaa
      541 tccacgaggt cttcccagtg gcgcatccat tcgtcgagga gcgcacgacc tgctgtttcc
      601 atcacttgat agcacgtggc cattttatcg tccacccaac tcgaaacgta attgagaccc
      661 tcgggcgcca tgcgtccacg ctcccgaaac cgccgataaa ccgggactgc gtcgccattt
      721 cggaagtgct cgacaaccat gtacaagcgc acgccggttc tcctatgact atgcgcgttg
      781 agccgccgcc gaggagcgca gcgacgaggg aatcgcaagc gggcgaagcc cgcttgcggc
      841 ggtgggctcg agcgcgtggt taatcccaga agccatgcgg gaaccttcgg attccgcggc
      901 cgtcatttgg aaggtctaac gcacgcctct ttggtcccgg ggacacaact tccagcgcag
      961 acagctttgc cgctttgatc caccgtcgct cctccgcgcg gatgactgga acaaaggaca
     1021 tttccgcgtt gatctttgac gcagtaaccg ggaccacaga gctgctcgcc atattggtcg
     1081 ttcatcatgc cgcctccggg ctttgtacaa acgacctcac cgcgctgatt cgctgcgcag
     1141 gttgaatcgg gttggccaca gaccaccttt ccattctgat ccgtcacaca gccgccctgg
     1201 gcgagaacta gagtaggagc gatccaaaga gcggccgcgc cgactagaaa tttcgcaagc
     1261 ctattcacgc gatgtttcct tgagagtcgt cctagtgtta gtgctctgtc cggcgagcgc
     1321 gccacgtccg tgaaggcccc tggaaaccga agcccagaat tgtggaagga aggagtcgag
     1381 cgcgggcagt ttagcgcttc tcctcgccgc acgcgcgccc caattcctcg cgctcgtcag
     1441 cgctcaagtc ccggctgaac tggagcaccc ggtccaggat gtcgtcggtc tcgaagtaag
     1501 ccttgcggca gtcgaggtgc acgaatcctc ccggaacgaa gcgcccctct tcgtagaaca
     1561 cgagccggat gcgccagctc ccgcgcgcga tgggctcgcg gcagctgcgg catttcgcct
     1621 gtgcgcccgg cgagcgctcg gcgccgtcga tccgcgggag gcgccggtgc gcaaggctac
     1681 tgcgcgccgc gcgctcaatg ctctcccggt cgggcacgtt cgctggcgtt tccactagag
     1741 cctgaagcag gggctccggc cgcttgtagg ccgcgcagtg cggatgcaac cacacagtca
     1801 tctcgccctc cgcaaagggg ttgggcaggc gctcgccgaa gcgcaggtct ccacgctgga
     1861 tggcctgagc gcagccgcgg cacttggagc gaccgctcgt ggcggtttcg aagaggtgag
     1921 acacgcaggg aactcaccac gcgctacgcg gcccgcgcct ccacctcggc attctcccgc
     1981 gcaagaaacg cgtggatctg cgcgaccacc gccgcgccct cgccgatcgc gccgccgacg
     2041 cgcttgaccg agcccgagcg cacgtcgccg atcgcgaaca cgccgggcac gctggtttcg
     2101 agcggcaggc gcccgccggc gccggtcctg acgaagccct tgtcgtcgaa ggcgacgtcg
     2161 caatccttca gccactccgt cgccggatcg gcgccgagga acaggaacac gttgcggatc
     2221 gcgcgggact cctcggcgcc cgagtcgcgg tgcctccagc gcagcgactc gacgccggac
     2281 tcgcgcgagc cctcgagcgc gacgatctcc gtccgcgtgc gcaattcgat gttggggatc
     2341 gcggcgatcc ggtcgatcag atatctcgac atgctcgcgg cgagccccgg gccgcgcacc
     2401 agcatccaca ccttggcgac atgcccggcg agaaacaccg cggcctgccc cgcggagttg
     2461 ccgccgccga cgagcgccac ttcctgcccc gcgcacgcct tcgcctcgat gggcgaggcc
     2521 cagtaccaga cgccgcgccc ttccagggcc ttcaggtcgg cgcagtccgg tcggcgataa
     2581 cgggcgcccg tggcgacgac caccgtgcgg ctgcgcacgc cgcgcgtgcc ggcgagctca
     2641 agcgcgagcg gatcgcgctc ctccggccgg ccgcaatcca ggcgcaccgc ctcggcgggg
     2701 atcagcatgc gcgcgccgaa tttctgcgcc tgcgtatagg ctcggcccgc aagcgcctgg
     2761 cccgagatgc cggtcgggaa gccgagatag ttctcgatgc gcgcgctcgc gcccgcttgc
     2821 ccgccgaagg cgcgcgcgtc gatgacgagc accgagaggc cttcggaggc cgcgtagacc
     2881 gcggtcgcga gtcccgcggg gcctgcgccg acgacggcga cgtcgtgcac gcgctccgcg
     2941 tcggcggcat ccagcatgcc gatgcaccgc gcaagctcgt tctccccggg attgcgcagc
     3001 accgaaccat cggggaaggc gacgagcggc atgtctccgg gcctagccgc gtagcgctcg
     3061 atgaaaccgt gtgcatccgg gtcgctcgcc ggatcgaaga cgaggtgcgg gatgccgttg
     3121 cgcgtgagaa aacttcgcag ccgcgccacg tccggggacg aaggcgcgcc gatcagcacc
     3181 gggccgcctg cgcccatctc gatcaggccc acgcggcgca ggatcagcgc gcgcatgatc
     3241 tcctcgccga gatcggcttc ggcgatcagc agcgcgcgca acagctccgg ggcaacgagg
     3301 agcgcttcga cagcgccgac tgcaacgccg tccgcgagcg cgcgccgccc ggagagctgc
     3361 ccgatttcgc ccgagaatcc tcccgcggac agctcggcga tgagcagatc gtggccgtgc
     3421 ccgtcgcggc cggtgatgcg cagcgtgccc gagagcacga cgaacatgcc cgggctggtt
     3481 ttgccggtct cgaacaggca ctcgccgtcc gcgtagcggc gcggctcacc gaaccgccgc
     3541 aggcgcgcga tctcctcacc cgcgaggacc gggaacatct gcgcgcggcg cgctgctagc
     3601 gtcggggatt ggtagggctc agccatcgcg gcggggatcc cgctcctcgt tttcagttcc
     3661 atgggatgat aaccccggtg cgttcgcaaa gcgatgcaag ccttgcgtac gccggccccc
     3721 gcccccactt tctcccagtg agattcccac gccccgttgt cgtcctgctc gctctcctgc
     3781 atatctatat cggcttgcgg ctgcttctgc cgttcgacgc tgtcatccag cttgtgggcg
     3841 gcgccttgct cgcggtgtgt ttctggcttt tgcccaaggg attccggatc cgggacgacc
     3901 gcggcgcatg gggcgtgatg ctcccgtggc tgacgatggg tttcttctcg tggctgctgg
     3961 tcctgaccgt gtttcgcgac gtgagcctcc tggtgtccgc ctttgcgctg tcagcgcagg
     4021 cccacgagtc ctgggtccgc atttcggcga taggcgtcat ggcgctcacg ccggc
//
LOCUS       DASPZD010000157         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1354109, whole genome shotgun sequence.
ACCESSION   DASPZD010000157 DASPZD010000000
VERSION     DASPZD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354109"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VEM33_03005"
     CDS             <1..632
                     /locus_tag="VEM33_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50825.1"
                     /translation="KRRALDLLKEAPLRRTLIMSGVTLTGIELFSFYMPVYGQSIGLS
                     ASRIGLVLSAYAAAAFAVRLLMQKLVRRFTEAGVLTGSLFIATATYALFPFLSGVPML
                     VLTAFLLGLGLGSAQPLTIMLTYHHAPPGRSGEALGMRLTVNKITQISIPLVFGGIGA
                     VFGLAPVFWANAAFLLAGGIVSLKDLREGRGSSVSRARDTRGSDLRKER"
     gene            complement(559..1035)
                     /locus_tag="VEM33_03010"
     CDS             complement(559..1035)
                     /locus_tag="VEM33_03010"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYS50826.1"
                     /translation="MSAAGRAAARWLSSTSNRTFVLWPVVIFAAEAALQQGIPWIEIW
                     GLPLLAWGYLQYRLVGNYRLREGGGGPGISVPPERLVSGGPYRYCRNPMYLGHLIFLA
                     GLAFALDSWLAAALFVFHAAWFDRRVREDEARLSALFGDRYREYRARVKRWIPGLL"
     gene            complement(1032..>2159)
                     /locus_tag="VEM33_03015"
     CDS             complement(1032..>2159)
                     /locus_tag="VEM33_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS50827.1"
                     /translation="FYLILPIIGKELGLSYSQIGLIMTCQYIAGAVANIPGGVLVDTV
                     GRKGVLMAVSLFWVGFPYLLMGFTHSYLMLLGCVALVGFGNSLWHPTAIPTLARRFPE
                     RKGLVLSLHGMGGNVGDAIAPIVVGALLAVLSWREVVILNVVPGVVIALLLFVFLGTL
                     KLGGGKREASREPGAQSLREYFRGLPELFRNRGLILLSTSSAFRSMTQNALLTFLPVY
                     LAYEMGYSPFWVGTCMFALQTAGFAAAPVAGHLSDRMGRKSVMMTSMAMTAVVLVFMA
                     FAGKSLAFVFFVAVLGFFLYAIRPVLQAWLLETTPRNMGGTSIGVLFGAQALGSSIGP
                     LLGGVVADRYGLTATFAFLAGTIVAANLFILFMPKTEPATA"
BASE COUNT          420 a          702 c          712 g          325 t
ORIGIN      
        1 agaaacggcg cgcgctcgac ctgctcaagg aagctccgtt gcgccgcacg ctgatcatga
       61 gcggagtgac gctcaccgga atcgaattgt tctccttcta catgcccgtc tacggccagt
      121 cgatcggact ctcggcctcg cgcatcggac tggtgctgag cgcctacgcc gcggccgcgt
      181 tcgcggtgcg gctgctgatg cagaagctgg tccggcgctt caccgaagcg ggcgtgctca
      241 ccgggtcgct ttttatcgcg acagctacgt atgcactttt tccgtttctt tccggagtgc
      301 cgatgctggt gctgaccgcg ttccttctgg gcctgggcct gggctcggcg cagccgctga
      361 ccatcatgct gacctaccac cacgctccgc cgggacgttc gggcgaagcg ctgggaatgc
      421 gcctcaccgt caacaagatc acccagatct cgatcccgct cgtgttcggc ggcatcggcg
      481 cggtgttcgg ccttgctccg gtgttctggg ccaatgccgc tttcctgctc gccggaggaa
      541 tcgtctcgct gaaggatctc agagaaggcc ggggatccag cgtttcacgc gcgcgcgata
      601 ctcgcggtag cgatctccga aaagagcgct gaggcgcgct tcgtcctcgc gcacgcgccg
      661 gtcgaaccac gccgcgtgga acacgaacag tgccgcggcg agccacgagt cgagcgcgaa
      721 agcgagcccc gcgagaaaaa tcaggtgccc gaggtacatc gggttgcggc aatagcgata
      781 cggtccgccg ctgaccaggc gctcgggggg aaccgaaatc ccgggcccgc cgccgccttc
      841 gcgcaagcga tagttaccga cgaggcgata ttgcaggtat ccccaggcga gcagcggcag
      901 gccccagatc tcgatccacg gaatgccctg ctgcagcgcc gcctcggctg cgaagatcac
      961 gaccggccag agaacgaaag tgcggttcga ggtgctggag agccatctcg ccgcggcgcg
     1021 gccggcggcg ctcacgccgt cgccggctcg gtcttgggca tgaacaggat gaagagattc
     1081 gcggcgacga tggtacccgc gagaaaggcg aacgtcgcgg tcagcccgta gcggtcggcg
     1141 accacccccc cgaggagcgg cccgatcgag gagccgagcg cctgcgcgcc gaagagcacg
     1201 ccgatgctgg ttccgcccat gttcctcggc gtcgtctcga ggagccacgc ctgcaggacc
     1261 ggccggatcg cgtaaaggaa gaaaccgagg accgcgacga agaaaacgaa tgcaagcgat
     1321 ttaccggcaa acgccatgaa gacgagcacc accgcggtca tcgccatgct ggtcatcatc
     1381 acgctcttgc ggcccatgcg gtccgacagg tgcccggcca cgggcgcggc ggcgaagccc
     1441 gcggtctgca gcgcgaacat gcacgtgccc acccagaagg gcgaatagcc catttcgtag
     1501 gcgagataga cgggcaggaa agtcagcagc gcgttctgcg tcatcgagcg gaacgccgag
     1561 ctcgtcgaaa ggaggatgag cccgcggttg cggaacagct cgggcaggcc gcggaaatat
     1621 tcgcgcaggg actgcgcgcc cggctcgcgc gaagcctcgc gcttgcctcc gccgagcttg
     1681 agcgtgccga gaaagacgaa caggaggagc gcgatcacga ctccgggaac gacgttcagg
     1741 atcaccactt cgcgccagga aagtacggcg agcagcgccc ccacgacgat gggggcgatc
     1801 gcgtcgccga cgtttccgcc catgccgtgc agcgagagca cgaggccctt cctctcgggg
     1861 aaacggcgcg cgagcgtggg aatcgccgtc ggatgccaca ggctgttgcc gaagccgacg
     1921 agcgcaacgc agcccagaag catcagatag ctgtgcgtga agcccatcag cagatacggg
     1981 aaacccaccc agaagagcga gaccgccatc agcacgccct tgcgtccgac ggtgtcgacc
     2041 agcaccccgc cggggatgtt cgcgacggcg cctgcaatat attggcaggt catgataagg
     2101 ccgatctggc tgtaggaaag gcccaattcc ttcccgatga tgggcaggat cagatagaa
//
LOCUS       DASPZD010000158         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_27861, whole genome shotgun sequence.
ACCESSION   DASPZD010000158 DASPZD010000000
VERSION     DASPZD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27861"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1632
                     /locus_tag="VEM33_03020"
     CDS             286..1632
                     /locus_tag="VEM33_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50828.1"
                     /translation="MKHKSILSVGVFILGAAAFFAESSFLAGGNALADDDSHSKGDRR
                     GDDQAQSTIDLGRQIFRFDTFGDEGWWGDTLKLHQALAGAKNGGVGPGVSPKTALAVG
                     LKVDADALPWQLKSQIRAGRVNLDDPATTLALLKLNAVVGVTGIFDGAGNIKSMGVQC
                     ALCHSTVDDSFAPGIGKRLDGWANRDLNVGLIVSLAPNLTPIADLLQVDVATVKAVLN
                     SWGAGRFDAELDKDGKAFRPDGGQAGTLIPPAFGLAGVNLHTWTGFGSVPYWNAYVAV
                     TEMHGSGTFFDQRLSDPVQYPVSARSGSWNTRGTPDLVTAKLPALRAYQLSLKAPTPP
                     VGSFDVAAAARGKEVFDDQGKCATCHVPPLYTEPGHNLHSPAEIGVDSFQADRSPTHM
                     YRTAPLAGLWTHQKGGFYHDGRFAALRDVVEHYDSQFMLGLRTQEKTDLIEYLKSL"
     gene            1767..>2140
                     /locus_tag="VEM33_03025"
     CDS             1767..>2140
                     /locus_tag="VEM33_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYS50829.1"
                     /translation="MQPATVAIAYGKLSDPDLAQRIASGDKDAFELLMRRHNQILYRT
                     ARSILKDDAESEDTVQEAWLLAYRAIGNFRGDAKLSTWLVRIVVNEAIGRARKRSRGA
                     EVIQLSGESLEDRETVEGNMDKV"
BASE COUNT          412 a          659 c          696 g          373 t
ORIGIN      
        1 gggcttgagt gcggcaccga gctgctcgag cgaggcggcc gcagtctggg cgccgcggat
       61 cggctgtgcc aggtaggtgg ccaggcgtcc gcccatccat cgaagtaccg ggttcatggc
      121 tcattcgcgt gtagtcacgc acctttcgtg agtagcgatt cgatgacgct tcggcggtcg
      181 gcgttccgga atccgtggaa cgcattctaa gcgggaacgt cgggattgcg accttcatcc
      241 aatacacgca cgcaagggag caaatcaatt cgaaaaggag tttcgatgaa acacaaatcc
      301 atcctgtcag tgggggtttt tattctaggt gcggctgcat tctttgcaga gtccagcttt
      361 ctcgccgggg gaaacgccct ggctgacgac gactcgcatt cgaagggcga ccggcgaggc
      421 gacgaccagg cgcagagcac gatcgatctg ggcaggcaga tatttcgttt cgataccttc
      481 ggcgacgagg gctggtgggg cgatactctc aagctgcacc aggcgctcgc cggcgcgaaa
      541 aacggcggcg tcggtccggg cgtgagcccg aagacggctc tcgcggtcgg cctcaaggtg
      601 gacgcggacg cgctgccctg gcagctgaag tcgcagatca gggcgggcag ggtcaatctc
      661 gacgatccgg cgacgacgct cgcgctgctc aagctcaacg ccgtggtcgg cgtgacgggc
      721 attttcgacg gcgccggaaa catcaagtcg atgggcgtgc aatgcgcgct ctgccattcg
      781 acggtcgacg actccttcgc gcccgggatc ggcaagcgcc tggacggctg ggcgaaccgc
      841 gacctgaacg tcggcctgat cgtctctctc gctcccaatc tcaccccgat cgccgatctc
      901 ctgcaagtcg acgtggctac ggtgaaggcg gtgctgaaca gctggggcgc ggggcgcttc
      961 gacgccgagc tcgacaagga cggcaaggcc ttccggcccg acggcgggca ggcgggcacg
     1021 ctgattccgc ccgcattcgg tctcgccgga gtgaatctcc acacctggac tggcttcgga
     1081 tccgtcccct attggaacgc ctacgtcgcc gtgacggaga tgcacggctc ggggactttc
     1141 ttcgaccagc gcttgagcga tcccgtgcag tatccggtgt ccgcgaggag cggctcatgg
     1201 aacacgcgcg gcactccgga cctcgtcacg gcgaaactcc cagcgcttcg cgcctatcag
     1261 ctctcgctca aggcgccgac tcctccagtc gggagtttcg acgtggccgc cgcggcacga
     1321 ggcaaggagg tcttcgatga ccagggcaag tgcgcgacct gccacgttcc gccgctgtat
     1381 acggaaccgg ggcacaacct gcactctccg gccgagatcg gggtcgattc cttccaggcg
     1441 gatcgttcgc ccactcatat gtaccgcacg gcgccgctcg cgggcttgtg gacacaccag
     1501 aagggcggct tctatcacga cggacgcttc gccgccttgc gcgacgtggt cgagcactac
     1561 gacagccagt tcatgctcgg cttgcggacg caggaaaaga ccgacctgat cgaatacctc
     1621 aagtcactgt agaaattgaa aataacgggg cacggggacc gatggtcctt cgagtgcccc
     1681 gtttcctttc agcggaccgg gaacgcgggg cgcacaatct tcatccaatg gagcaaaacc
     1741 ggcgtaagaa agagcaagag gtgtctatgc aacccgcaac cgtcgcaatt gcttacggca
     1801 agttatccga cccggacttg gcgcagcgca tcgcgtccgg cgacaaggat gcattcgagc
     1861 tgctgatgcg ccggcacaac cagatcctct accgcacggc gcgcagcatc ctgaaggacg
     1921 atgccgagtc cgaggatacg gtgcaggaag cctggctgct cgcgtaccgc gcgatcggaa
     1981 attttcgcgg cgacgcgaag ctctccacgt ggctggttcg catcgttgta aacgaggcga
     2041 tcgggcgcgc tcgcaagcgc agccgcgggg ctgaagtcat tcaactgagc ggagagtcgc
     2101 tggaggatcg cgaaaccgtg gagggaaaca tggacaaggt
//
LOCUS       DASPZD010000159         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1132188, whole genome shotgun sequence.
ACCESSION   DASPZD010000159 DASPZD010000000
VERSION     DASPZD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132188"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VEM33_03030"
     CDS             <1..632
                     /locus_tag="VEM33_03030"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYS50830.1"
                     /translation="KAAIFQTGVGTKALFAATDSLGLTPNLLGLLANCVVVARGPKPT
                     GALRSREVRIDRSAKEPYTTVEVLESLEDLSFRGESVVVQRYGESNVELEKALRAKGA
                     EVVEIPTYRWALPEDSGPLAGLIDALDRAQVDATVFTSASQAHNLFALAQKLGRDGPL
                     AANLSRTLVASIGPVCSAALREHGVKIALEAKPPKLGPLVAALDRALSA"
     gene            698..>2359
                     /gene="typA"
                     /locus_tag="VEM33_03035"
     CDS             698..>2359
                     /gene="typA"
                     /locus_tag="VEM33_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HYS50831.1"
                     /translation="MSRSIRNLAIIAHVDHGKTTLVDKLLHQSGTFAAHQHIAERVMD
                     SNELERERGITILAKNTAVDYEGVHINIVDTPGHADFGGEVERSLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALALGLNPILVVNKIDRPGARPDWVVNATFDMMDKLGASEHQLDF
                     PVVYTSALLGTATLALAQPGSDMKPLFHTIIERIPAPRGDPDGPLQLQISNLDYSSYV
                     GRIGIGRISRGKLRPSQEVLVLNADKAPVKAEVGQVMGFRGLERIPMEEAVAGDIVLV
                     TGVEETHIGVTLADPLQPEALPMLKVDEPTLTMNFQVNTSPFAGQEGKFVTSRQLRER
                     LLRELQGNVALRVEETADADVFRVSGRGELHLTILLENMRREGYELAVSRPRVVIKEI
                     AGVRHEPFEVLTVDVEEQHQGAVMEALGARRGDLLDMMPDGRGRVRLDYRISARGLIG
                     FQSDFLNLTRGNGLMSHVFDEYAPAKPEIPSRRNGVLISAEEGEAVAYALWKLQERGR
                     MLVSPGERVYEGMVVGLHSRDNDLVVNPVREKHLTNIRAAGKDEAI"
BASE COUNT          430 a          791 c          773 g          365 t
ORIGIN      
        1 cgaaagccgc gatcttccaa accggcgtgg gcacgaaggc gctttttgcc gccaccgatt
       61 cgctagggct gaccccgaac cttctcggct tgctggcgaa ctgcgtggtg gtcgcgcgcg
      121 gccccaagcc tacgggcgcg cttcgctcgc gcgaagtgcg catcgatcgc agcgccaagg
      181 aaccctatac gaccgtcgaa gtgctggaat cgctcgagga cctgtcgttc cggggcgaaa
      241 gcgtcgtggt ccagcgttac ggcgagagca acgtggagct ggaaaaagcg ctgcgcgcga
      301 agggcgcgga agtcgtggaa atacccacct accgctgggc gctccctgag gacagcggac
      361 cgctcgcggg gctgatcgac gcgctcgatc gcgcgcaggt cgacgctacc gtgttcacca
      421 gcgcgtcgca ggcgcacaac ctgttcgcgc tcgcccaaaa actcggcaga gacggccctc
      481 tcgccgcgaa cctgagccgg acactcgtcg cctccatcgg tccggtgtgc agcgccgcgc
      541 tgcgggagca cggcgtgaaa atcgcactgg aggcgaaacc gcccaagctc gggccgctgg
      601 tggcggcgct cgaccgggcg ctgtcggcgt aacgcgtttc cgctgctgca ccgcgcaaac
      661 tgatacaatg cgcgactctt tttcgcatcc cgcctgaatg tctcgttcca tccgcaacct
      721 cgcgatcatc gcccacgtcg accacggcaa aacgacgctc gtagacaagc tgctgcacca
      781 gtccggcacg ttcgccgcgc accagcacat cgcggagcgg gtcatggact ccaacgagct
      841 cgagcgcgag cgcggcatca ccatcctcgc caagaacacc gccgtcgact acgagggcgt
      901 gcacatcaat atcgtcgaca cgcccggcca cgccgacttc ggcggcgaag tcgaacgctc
      961 gctctcgatg gtcgacgggg ttctgctgct ggtggacgcg gtcgaaggcc cgatgccgca
     1021 gacgcgcttc gtcacgcgca aggcgctcgc cctaggcttg aatccgatcc tggtggtgaa
     1081 caagatcgac cgtcccggcg cgcggcccga ctgggtggtg aacgccacct tcgacatgat
     1141 ggacaagctc ggcgcatcgg aacaccagct cgactttccc gtggtctata cctcggcgct
     1201 gctcggcacg gctacgctgg ctctcgcgca acccggcagc gacatgaagc cgctcttcca
     1261 cacgatcatc gagcgcatcc cggcgccccg aggagatccg gacggacctc tgcagctgca
     1321 gatcagcaac ctcgactatt cgagctacgt ggggcgcata ggtatcgggc gcatcagccg
     1381 cggcaagctc aggccctctc aggaggtgct ggtgctgaac gcggacaagg cgccggtgaa
     1441 ggccgaggtc ggccaggtca tgggcttccg cggtctggag aggatcccga tggaagaagc
     1501 ggtcgcgggc gatatcgtgc tcgtcaccgg ggtggaggag acccacatcg gcgtcacgct
     1561 cgccgacccg ctccagcctg aagcgctgcc gatgctcaag gtggacgagc ccacgctcac
     1621 catgaatttc caggtcaaca cctcgccctt cgccgggcag gaaggcaagt tcgtcaccag
     1681 ccgccagctg agggagcgcc tgctgcggga gctgcagggg aacgtcgcct tgcgcgtcga
     1741 ggagactgcc gacgccgacg tgttccgggt ctcgggacgg ggcgagctcc atctcaccat
     1801 tctgctcgag aacatgcgcc gcgaaggcta cgagctggcc gtgtcgcggc cgcgggtcgt
     1861 gatcaaggaa atcgcaggcg tcaggcacga gcccttcgaa gtcctcaccg tcgacgtcga
     1921 ggagcaacat cagggggcgg taatggaagc gctcggcgcg cgccgcggcg acctgctcga
     1981 catgatgccc gacggccgcg gccgcgtccg gctcgactac cggatttcgg cgcgcgggct
     2041 gatcggcttc cagtccgatt ttctcaatct cacgcgcggc aacggcctca tgagccacgt
     2101 gttcgacgaa tatgccccgg cgaaacctga gattccctcg cggcgcaacg gcgtgctgat
     2161 ctcggccgag gagggcgagg cggtcgctta cgcgctgtgg aagctgcagg aacgcggacg
     2221 catgctagtg agccccgggg agcgcgtgta cgaaggtatg gtggtgggcc tgcacagccg
     2281 cgacaacgac ctcgtggtga accccgtccg cgagaagcat ctcaccaata tccgcgccgc
     2341 gggcaaagac gaggcgatc
//
LOCUS       DASPZD010000160         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_760745, whole genome shotgun sequence.
ACCESSION   DASPZD010000160 DASPZD010000000
VERSION     DASPZD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760745"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VEM33_03040"
     CDS             complement(<1..586)
                     /locus_tag="VEM33_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50832.1"
                     /translation="MTWLGRLFQKHGGRRKSLTWHISFLFALISFLVIGAMGFSIDRM
                     LAGELLEANDVLLLGNMSLLRGRLARLGPSETVLTSPRFVDEAGMGYRKLAVAVLDED
                     RRILRASSEFNIPVSALPEKAIPIEAIPDRVDNALERELRTRFGGLSRQWTAPDGHWY
                     QVLLARLPLASGTSSEPQSELLALSYDRSLPKELL"
     gene            complement(583..1257)
                     /locus_tag="VEM33_03045"
     CDS             complement(583..1257)
                     /locus_tag="VEM33_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HYS50833.1"
                     /translation="MRILIVEDEPKTAAYAKKGLTEHGFVVDIAANGVDGRHLALSSD
                     YDLVILDIMLPGADGWAVLHEIREKKQTPVLFLTARDDVADRVRGLELGADDYLVKPF
                     AFSELLARVRSVLRRGAKQASESLRIGDLEIEPRRRRVSRGEKKIDLTAKEFALLDYL
                     VRNAGEVLSRTLIAEHVWDMNFDSDTNVVDVAMRRLRAKVDDPFEVKLIRTIRGVGYV
                     LEEPLA"
     gene            complement(1338..1667)
                     /locus_tag="VEM33_03050"
     CDS             complement(1338..1667)
                     /locus_tag="VEM33_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CzcE family metal-binding protein"
                     /protein_id="HYS50834.1"
                     /translation="MKTLVKAVAVLALSAASLSAVALTNADRYGEAATPAAAERTIVI
                     GANTRFVNVNHGEIVKFVANGQEFAWDFDGVPQAFDLKQVAPQGAIDHSVRVYIATTL
                     NDGGFGD"
     gene            complement(1840..>2448)
                     /locus_tag="VEM33_03055"
     CDS             complement(1840..>2448)
                     /locus_tag="VEM33_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HYS50835.1"
                     /translation="GVLVVSEAAYLERPGNAEPRNRRLRTGRFSELTPYDDKYAIGLW
                     HYTATFEDLSAMDSSGEPVPHRGSSGGYLLVDRLLTRSSTPSDPRIAAFLQLGIGDAR
                     VNRFGSYAGAGLVVSGLIPGRHDELGIAVASARNGSHYLEQQERLGTPAQRSETAIEL
                     SYLAQISKSIAVQPDLQYVVHPNTDPAISNALAFLLRFEVSF"
BASE COUNT          435 a          787 c          793 g          433 t
ORIGIN      
        1 caagcaattc cttgggcagc gaacggtcgt acgagagcgc aagcagctct gactgcggct
       61 cgctgctcgt gccggaagcg agcggcaggc gcgcgagcag cacctgatac caatgcccgt
      121 ccggtgcggt ccactggcgg ctcaagcccc cgaagcgcgt gcgcagctcg cgttccagcg
      181 cgttgtccac gcggtctgga atcgcttcga tggggatcgc cttctcgggc agcgccgaaa
      241 cggggatatt gaactcgctc gaagccctga gaatgcggcg gtcctcgtcg agcactgcaa
      301 cggcgagctt ccggtacccc atcccggctt cgtcgacaaa ccggggactg gtgaggaccg
      361 tctccgaagg tcccagacgc gcgagcctgc cgcgaagcag gctcatgttg ccgagcagga
      421 gcacgtcgtt cgcctccagc agctcgcccg caagcatgcg gtcgatgctg aatcccatcg
      481 ccccgatgac caggaacgag atgagcgcga acaaaaagct gatgtgccaa gtgagcgatt
      541 tgcgccgccc gccgtgtttc tgaaagagcc gcccgagcca ggtcacgcga gaggctcctc
      601 gagcacgtat cccacgccgc ggatggtgcg gatgagcttc acctcgaacg gatcgtcgac
      661 cttcgcgcgg aggcgccgca tcgccacgtc gaccacattg gtgtcgctgt cgaagttcat
      721 gtcccagaca tgctcggcga tgagcgtccg ggagagcacc tccccggcat tgcggaccag
      781 atagtcgagc agcgcgaatt ccttcgcggt gagatcgatc ttcttttcgc cccgggaaac
      841 cctgcgcctg cgcggctcga tctccaggtc gccgatcctc aggctctcgc tggcctgctt
      901 cgctccgcga cgcagcaccg agcgcacgcg cgcgagcagc tcggagaacg cgaagggctt
      961 gaccaagtag tcgtccgcgc cgagttcaag gcccctgacc cggtcggcga cgtcgtctcg
     1021 cgccgtcagg aacaggaccg gggtctgctt cttttcgcgg atctcgtgca gcaccgccca
     1081 gccgtcggcg ccggggagca tgatgtcgag aatgaccagg tcgtagtccg aggacaacgc
     1141 cagatgccgg ccgtcgaccc cgttcgcggc gatatcgacg acgaagccgt gctcggtgag
     1201 cccctttttc gcgtaagccg ccgtcttggg ctcgtcttcg acgatgagga tgcgcatggt
     1261 cgcgagcgaa agaaaagaaa gaaaaaggcg ccgggacttc ggcgcctttt cattctactc
     1321 cacagagacc gtcgcgatta gtcgccgaag ccgccgtcgt tcaacgttgt cgcgatatag
     1381 acgcgcacgc tgtggtcgat ggcgccctgc ggggcgactt gcttcagatc gaaggcttgc
     1441 ggtacgccgt cgaagtccca ggcgaattcc tggccgttcg ccacgaactt cacgatttca
     1501 ccgtggttca cgttcacgaa gcgggtgttc gcgccgatga caatggtgcg ttcggctgcg
     1561 gcgggggttg cggcctcgcc atagcggtcg gcattggtca gggcaacggc agacaggctc
     1621 gccgcggaca gcgcgagtac ggcaacggct tttactagcg tcttcatggc atttctccaa
     1681 ttcatcaagt tgaggggcat ccagagcgcc tgggttgccg gcgccctgta tgtgccgcca
     1741 ctttagaacc cagccacgga cggatagctg accgcgaaat tacatttttg taatgttccg
     1801 gcgccgcgga gcggtccggg gttctgtgat gtatcgaccc tagaacgaca cctcgaaccg
     1861 aagcaggaac gcgagcgcgt tgctgatcgc cggatccgtg ttcggatgaa cgacatattg
     1921 cagatccggc tgtacggcga tcgacttcga gatctgggcg agataactca gctcgatcgc
     1981 ggtttccgat ctttgcgccg gcgtgccgag ccgctcctgc tgctccaggt agtgcgagcc
     2041 gttgcgtgcc gacgcgacgg cgatgcccag ctcgtcgtgc cggccgggaa tcagaccgga
     2101 gacgaccagc ccagcgccgg cataggaacc gaagcggttc acgcgcgcgt cgccgatgcc
     2161 gagctggagg aaggccgcga tccgcgggtc ggacggcgtg ctcgatcgcg tcagcaggcg
     2221 gtcgacgagc agatagccgc cgctcgagcc gcgatgcggc acgggctcgc cgctcgagtc
     2281 catcgcgctc aggtcttcga aggtcgccgt gtagtgccag aggccgatcg cgtacttgtc
     2341 gtcgtaaggc gtcagctccg agaagcgccc ggtgcgcagc ctccggttcc tcggctccgc
     2401 gtttcccggc cgctcgagat acgcggcctc cgatacgacg agcacgcc
//
LOCUS       DASPZD010000161         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1110506, whole genome shotgun sequence.
ACCESSION   DASPZD010000161 DASPZD010000000
VERSION     DASPZD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110506"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VEM33_03060"
     CDS             <1..151
                     /locus_tag="VEM33_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747959.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HYS50836.1"
                     /translation="IAIGHPLGASGARLVTTATYQLQAIRGRYALCTMCIGVGQGIAL
                     IIERV"
     gene            complement(167..967)
                     /locus_tag="VEM33_03065"
                     /pseudo
     CDS             complement(167..967)
                     /locus_tag="VEM33_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            1097..1639
                     /locus_tag="VEM33_03070"
     CDS             1097..1639
                     /locus_tag="VEM33_03070"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HYS50837.1"
                     /translation="MHTEVHVHGTVLLKSGVSQTDIELALRHWFDYVDVDGLADARSA
                     HQDEPGIVFDRRRRSLDICWTGYVGRNFQHSLEGAFESLSPYSEEAAAVEVSYYQEDG
                     QDEHSWVFVGPTPESIRDMQKRRMIDDLSALLSRHFGDAEIGEVTALVNQLFTRRGAE
                     TPQDRTQVRSGGVSGKKHLH"
     gene            1931..2374
                     /gene="mraZ"
                     /locus_tag="VEM33_03075"
     CDS             1931..2374
                     /gene="mraZ"
                     /locus_tag="VEM33_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYS50838.1"
                     /translation="MFQGATELSLDAKGRLAVPTRYRDALLPGGRAVVLTAHPDSCLL
                     LYPRVAWEPIGAKVQALSSFNEQTRWWQRLLVGFAEELELDAQGRILVSPALRKFAGL
                     KKDVMLVGQGSHFEIWDVASWDERLAQSLTQVGTNPPPGTENFSL"
     gene            2371..3228
                     /gene="rsmH"
                     /locus_tag="VEM33_03080"
                     /pseudo
     CDS             2371..3228
                     /gene="rsmH"
                     /locus_tag="VEM33_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402052.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
BASE COUNT          578 a          983 c          999 g          668 t
ORIGIN      
        1 gatcgcgatc ggccatccgc tcggcgcatc tggcgcgcgc ctcgttacta cggcgacgta
       61 tcagctccag gcgatcaggg gccgctatgc cttgtgcacc atgtgcatcg gcgtgggaca
      121 agggatcgcg ctgatcatcg aacgggtttg actgctagtg cgcgacgctc ttgacgatgt
      181 agccgcccga tttctcgtcg cgctcgagct gcagcatctt gcgcgactcg gcttcatcca
      241 ggagcgttga gaacgacgag aagccgtaat aggactcgtt gaaaccgggg ttgcggcgct
      301 tcagcgcctg cttcaccatc gatccccaga tcttctcgtc ggcaccgcgc tcctcgataa
      361 gcgcctcgac cgtctgcatc acgagctcca gcgccttctc ggtcttgtcc tcgccctctt
      421 tcggagtgcc ttccccgctt gcggcgggct tcgcctgtgg cttcttcttc ggctgccggt
      481 gccctggctt ttcccgcacc agatcgtcgt agaagatgaa ctcgtcgcag ttcgccacca
      541 gcagatcgga gctggagttc ttcacgccca ctccgatcac ggtcttgtcg ttttcgcgca
      601 gcttcgagac gagcggcgaa aaatccgagt cgccgctgat gatgacgaaa gtatcgacgt
      661 gcgacttggt atagcaaagg tccagcgcat cgaccaccat ccggatgtcg gccgaattct
      721 ttccggacat gcgcagatgc gggatttcga tgagctcgaa cgaggcttca tgcatgtcgg
      781 atttgaactc cttgtaccgt tcccagtcgc aataggcttt cttgaccacg atgctccctt
      841 tcaggagcag gcgctcgagc accttgttga tgtcgaactt ggcgtatttt gcgtcgcgca
      901 cgccgagcgc gacgttctcg aagtcgcaga aaagcgccat gtttacggtg tcgctcggga
      961 cggtcatttc ggctccttga aactctttac ggcttccgca ttatcccatt ccccgtgttc
     1021 aagacgcggc ccgcgcccca aaagggttga agtcccggct ttctcggact agcatcggtg
     1081 tttcgggggc aggcaaatgc acactgaagt tcatgttcac ggcacggtgt tgttgaaaag
     1141 cggcgtcagt cagacggaca tcgagctggc gctcagacac tggttcgact acgtcgacgt
     1201 ggacggtctc gccgatgctc gcagcgccca tcaggacgag cctggcatcg tgttcgacag
     1261 gcgtcggcgc tcgctcgaca tctgctggac gggatacgtc gggcgcaatt tccagcattc
     1321 gctcgagggg gcattcgagt ctctgtcgcc ctactccgag gaggcggctg cggtggaggt
     1381 gagctattac caggaggacg gacaggacga gcacagctgg gtgttcgtcg gcccgacgcc
     1441 cgagtcgatc cgcgatatgc agaagcggcg catgatcgac gacctctcgg cgcttctttc
     1501 gcgccatttc ggcgacgccg aaatcggcga ggtgaccgcc ctcgtcaacc agcttttcac
     1561 tcgccgcggc gccgagacgc cacaggaccg gacccaggtc cgctccggcg gtgtttccgg
     1621 gaaaaagcac ctgcactaga aagcgccgga aaagtgggtc cgaccctccc ggcaaagagg
     1681 gtctcgagct ttttcattgg aaccctttga tttcctaatg tattacattc aatttacacg
     1741 ataacgcgtt aatttatata tccttatttt atatcaagca gattctcgaa aagccatctt
     1801 aagtccctgt tgtagctaaa aatttcatca atttcaggct tgaccgcctc ccctgtttcc
     1861 tctagagtgg taggaagtag gttttagtgg ggaactgtgg cgttccattt cgccactcga
     1921 agggggaacg ttgtttcaag gcgcgacaga gttgagtctc gacgccaagg gaaggcttgc
     1981 cgtgccgacc cggtatcggg acgccttgct accggggggg cgcgcggtgg tcctcaccgc
     2041 tcaccccgat agctgtctgc ttctttatcc gcgagtcgcg tgggaaccga tcggcgccaa
     2101 agttcaggcg ctctccagct tcaacgaaca aacccgctgg tggcagcggc tgctggtggg
     2161 cttcgccgaa gagctggagc tcgatgctca agggcgcatc ctcgtgtcgc ccgcgctgcg
     2221 caaattcgcc ggactgaaaa aagacgtgat gctggtgggg cagggaagcc acttcgagat
     2281 ctgggacgtc gcatcgtggg acgagcgact tgcgcagagt ctcacgcaag tgggtactaa
     2341 cccgccgccc ggcaccgaaa acttctcgct gtgagcgtcg atgcgcacct ccccgtcctc
     2401 ctccacccgg cagtcgatgc gctggcgatc gaatcaaagc gcgggggcac cttcgtagac
     2461 gcgacgttcg gcaggggcgg tcacagccgg tcgattctgg agcggctggg gccccgaggc
     2521 cggctgatcg ggatggaccg ggatccgtaa gcgatcgctg ctgcccggga tatacgcgac
     2581 ccgcgctttg cgccggtcca cgcgcgcttc agcgaactgc aggcaacgct ggtcgcgctg
     2641 catgcgggcg gtgccgacgg ggtgctgttc gatctgggcg tgtcctcgcc gcagctcgac
     2701 gacccgcggc gcggcttcag tttccgggtc gatgcgccct tggacatgcg catggacccg
     2761 acccggggcg agagcgcggc ggagtggctg cagcgcgctg aggaggcgga gatcagggag
     2821 gtgctgagcg tctatggcga agaacggttt gctaaacaga ttgcaaaggc gattgttgct
     2881 tctaggcgaa gagggcctgt ccggcggacc ggggaacttg ccgctctcgt ggctgcggcc
     2941 gtccggacgc gtgaaccggg gaaggatccg gcgacgcgga cgtttcaggc tctacggatt
     3001 cacctcaatc aggagcttga ggaattgtcg ctagcgctgc cgcaggcgct cactgccctc
     3061 aatccccatg gacgccttgc cgtgatcagc ttccattcac tcgaagaccg catcgtgaag
     3121 aacttcatgc gcggcgccgc gcggccgacg ctgcccgagc gtctcccggt aagagcccgg
     3181 gacctgccgc ctgcgaagct gcgcgtcatc ggccggccgg caaggccc
//
LOCUS       DASPZD010000162         6571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1142396, whole genome shotgun sequence.
ACCESSION   DASPZD010000162 DASPZD010000000
VERSION     DASPZD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142396"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VEM33_03085"
     CDS             complement(<1..514)
                     /locus_tag="VEM33_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYS50839.1"
                     /translation="MLRDWLGYAERRFRAGRLRFGHGTHGARDEAAWLLASVLRLPHD
                     ELARSLGYELTAQERRSASRLIAERVRTRSPLAYLIREAWLGEHRFYVDERVVVPRSF
                     IAELLRDRLSPWLARPRELRRALDLCTGSGCLAILLALAFPRAVVCATDLSRPALSVA
                     RRNLRAYRLGD"
     gene            complement(559..1704)
                     /gene="dapE"
                     /locus_tag="VEM33_03090"
     CDS             complement(559..1704)
                     /gene="dapE"
                     /locus_tag="VEM33_03090"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HYS50840.1"
                     /translation="MPAVEPAATLRLARELISRRSVTPEDGGCQELLAERLACLGFAI
                     EPVRRGEVSNLWARRGEARPLFCFLGHTDVVPTGPIEKWHSNPFMPTERDGKLFGRGA
                     ADMKTSIAAFVVAIEGFLARHPSPRGSIALLITSDEEGPAVDGTSKVVELLRSRGERI
                     DYCLVGEPTCVSRLGDTIKNGRRGSLCGTLRVRGVQGHVAYPHLAKNPVHLAAPALTE
                     LAATEWDRGNEYFPPTTWQISNIHGGTGATNVIPGEVEILFNFRFSTASSPEGLKARV
                     GSILDRHGLDYELDWSLSGTPYLTPRGKLVEALSAAIRDVTGATPEVSTTGGTSDGRF
                     VAQLCHEVVEFGPVNASIHKLDEHVDLSAIESLREIYQSLLGRLLAS"
     gene            complement(1770..2948)
                     /locus_tag="VEM33_03095"
     CDS             complement(1770..2948)
                     /locus_tag="VEM33_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYS50841.1"
                     /translation="MSAMQRLFQLMAEKKASDIFLSVGSPINIKINGVAMPINQKAMD
                     GNTVRGLLYEVIDDKQRKEYEDTLELNTAYTLQGIGSFRISAFRQKGSPAVVVRYIPN
                     TIPPLDSLGLPPVLKDIILEKRGLILMVGATGSGKSTTLAAMLDYRNEQRTGHIFTLE
                     DPVEFIFQNKKSIVNQREVGTDTLDMRVGLKNALRQAPDCILIGEVRDRETMTSAIQY
                     AQSGHLCLATLHANNSYHAMNRIISFYTLETRAALLQDLSATLKCVISQRLVKNVRGT
                     RSAAVEVMLNSRYMAELIEKGEINEMKEAMEKALTPGSQTFEQSLFKMIGDGLITQDE
                     GLANSDSANNLLWLINNTAAGADFAAGKERSPEARPSSLPSKAPFSEFKLDTSETERA
                     "
     gene            complement(2977..3813)
                     /gene="dapD"
                     /locus_tag="VEM33_03100"
     CDS             complement(2977..3813)
                     /gene="dapD"
                     /locus_tag="VEM33_03100"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HYS50842.1"
                     /translation="MSSPANQKLQSAVEEAWEDRERLSPGSAPSKTRKSVARVLDELD
                     RGGLRVAEKIDGAWRTHQWIKKAVLLSFRLEDNRPIRAGALRFYDKVASKFERYDAAR
                     FRRGGFRVVPPAAARHGAYIAKNVVLMPSFVNVGAYVDEGTMVDTWATVGSCAQIGKN
                     VHLSGGVGIGGVLEPLQANPTIVEDNCFVGARSEIVEGVIVEENAVISMGVFIGASTR
                     ILDRASGKILYGRIPAGSVVVPGTLPSADGKYGLYCAVIVKQVDAKTRAKTSINELLR
                     GE"
     gene            complement(3810..5015)
                     /gene="dapC"
                     /locus_tag="VEM33_03105"
     CDS             complement(3810..5015)
                     /gene="dapC"
                     /locus_tag="VEM33_03105"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HYS50843.1"
                     /translation="MRAGTVNPRLQSLQPYPFEKLRGLFEGAKPNPGLAHISLGIGEP
                     RHPTPELIKRALSENLAKLAAYPPTAGGDPLRQAIAEWLVRRYGLRAIDWKTEVLPVN
                     GSREALFAFAQTVVDPARGNSVVACPNPFYQIYEGAALLAGARAHYVNTLSKRGFAAD
                     YGAVPEEVWGRTQLLYVCSPGNPTGKVLDLDEWREIFSIADRHGFIVASDECYSEIYF
                     DEAAPPLGALQAATMLGRGFQRLVMFSSLSKRSNVPGMRSGFVAGDASVLEAFLLYRT
                     YHGGAMNPAVQAASVAAWNDEHHVRENRRKYAEKFARVTPMLQAALRVERPDAGFYLW
                     APTPIADTEFARRLYAEQNVTVLPGSFLARDSGGVNPGANYVRMALVAEIDECLEAAR
                     RIVEFTRKL"
     gene            complement(5012..5428)
                     /gene="queF"
                     /locus_tag="VEM33_03110"
                     /pseudo
     CDS             complement(5012..5428)
                     /gene="queF"
                     /locus_tag="VEM33_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025842.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
     gene            5543..>6571
                     /locus_tag="VEM33_03115"
     CDS             5543..>6571
                     /locus_tag="VEM33_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS50844.1"
                     /translation="MRLTQIRLAGFKSFVDPCTVSIPGQLVGVVGPNGCGKSNIIDAV
                     RWVLGESRASALRGDSMQDVIFNGSSLRKPVARASVELFFDNSLGRAAGQWSQYAEIS
                     VKRVLQRDGESSYFINNTHVRRRDVQDIFLGTGLGPRAYAIIEQGMISRIIEARPEEL
                     RVFLEEAAGVSKYKERRRETEHRLGGTRDNLARLSDIRQELGSQIERLGQQAETARRY
                     SELSAERAQKQNLLWLVRRNEALAEAQRHTREVERAAVELEAETARLRESERDLEQAR
                     TEHYAAGDALSAAQGELYGANADVSRIETEMRFVADTRQRLEAQLAQMRAQRDAGRRN
                     EIEQREALR"
BASE COUNT         1161 a         2047 c         2170 g         1193 t
ORIGIN      
        1 gatcgccgag acggtaggcc cgcagattcc gccgcgcgac ggagagcgca gggcgcgaca
       61 aatcggtggc acacacgaca gcgcgcggga aggccagcgc gagcaggatc gcgaggcagc
      121 ccgacccggt gcacaggtcg agcgcccggc gcagttcccg cggacgtgcg agccagggtg
      181 agagccgatc ccgaagcagc tcggcgatga aggagcgcgg cacgacgacg cgctcgtcca
      241 cgtagaaccg atgctcgccc agccaggctt ccctgatgag gtaggcgagt ggactgcggg
      301 tgcggacacg ctccgcgatc aaccgtgaag cgcttcggcg ttcctgcgcc gtcagctcgt
      361 agccgaggga tcgggcaagc tcatcgtggg gcaagcgcag cacgctcgcg agcagccacg
      421 ccgcttcgtc tcgcgcgccg tgcgttccgt ggccgaaccg gaggcggccg gcccggaagc
      481 gccgttccgc gtagcccagc cagtcgcgca gcactgccga agctgctgcg cgggtgcgtg
      541 tggaaggcct tgaacgcttc aggatgcgag aagccgcccg aggaggctct gatagatttc
      601 gcgcaaggat tcgatcgcgc tcagatccac gtgctcgtcg agcttgtgga tcgaggcgtt
      661 cacggggccg aactcgacca cctcgtgaca gagttgggcg acaaagcggc cgtcggacgt
      721 gccgcccgtc gtgctcacct ccggagtggc gccggtgaca tcccggattg cagcgctcag
      781 ggcctcgacc agcttgccgc gaggcgtgag ataaggcgtc ccggaaaggg accagtcgag
      841 ctcgtaatcg agtccatggc gatcgagtat cgaaccgaca cgcgctttca gtccttcggg
      901 cgagctcgcc gtggaaaagc ggaagttgaa caggatttcc acctcgcccg ggatgacgtt
      961 ggtcgctcca gttccgccat ggatgttcga gatctgccag gtcgtgggag gaaagtattc
     1021 gttgccccgg tcccactcgg ttgcggcgag ctcggtgagg gccggcgccg cgagatggac
     1081 gggattcttc gcaagatgcg gataggctac gtgtccttga acacctctca cgcgcaacgt
     1141 gccgcaaagc gaaccgcgcc ggccgttctt gatggtgtcg cccagcctgg aaacgcatgt
     1201 cggctcgccg acgaggcaat agtcgatgcg ttctccccgg ctcctcagca gctcgaccac
     1261 tttcgaggtc ccgtccactg cgggaccctc ctcgtctgaa gtgatgagca gcgcgatcga
     1321 tccccgaggc gagggatgcc tggccaggaa accctcgatc gccacgacga aagcggcgat
     1381 cgaggttttc atgtctgcgg ctccgcgccc gaacagcttt ccatcgcgct cggtgggcat
     1441 gaagggattg ctgtgccact tctcgatcgg accggtgggc acgacgtccg tgtgcccgag
     1501 gaagcagaag aggggacggg cttcgccgcg tcgcgcccag aggttgctca cctcgccgcg
     1561 ccggaccggt tcgatcgcaa atccgaggca cgcaaggcgt tcggcgagca attcctggca
     1621 gccgccgtct tcgggggtga ccgagcgtcg cgagatcaac tcgcgggcaa gacgcagcgt
     1681 cgcggccggt tcaacggccg gcatggtggt cttcggtgga accggcccag cagaacgaaa
     1741 cggggtggat cctgacgacc ggcatcaagc tatgcgcgtt ccgtctcgct ggtgtcgagc
     1801 ttgaactcgc tgaatggcgc tttgctgggg agtgaggacg gtcttgcctc cggacttcgc
     1861 tccttgcctg cggcaaaatc ggcgccggca gcggtgttgt tgatgagcca aaggagattg
     1921 tttgcggagt ccgagttggc cagcccctcg tcctgggtga tgagaccgtc cccaatcatc
     1981 ttgaagagcg attgctcgaa ggtctgcgag ccgggggtca gcgccttctc catcgcctcc
     2041 ttcatctcgt tgatctcgcc tttttcgatc aattcagcca tgtagcggct gttcagcatg
     2101 acctccaccg cagcggaacg cgttccccgt acgttcttga cgagacgctg cgatatgacg
     2161 catttgagcg tcgccgagag gtcctgcagc agcgcggcac gggtctccag cgtgtagaag
     2221 ctgatgatgc ggttcatcgc gtggtagctg ttgttggcgt gcagcgtggc gagacacaga
     2281 tgcccggatt gcgcgtactg gatcgccgag gtcatggtct ccctgtcgcg cacctcgccg
     2341 atgaggatgc aatcgggtgc ctggcgcagg gcgttcttca atcccaccct catgtccagc
     2401 gtatcggtcc cgacctcgcg ctggtttacg atcgacttct tgttctggaa aatgaactcg
     2461 accgggtctt cgagcgtgaa aatatgtccg gttcgctgct cgttgcgata gtcgagcatg
     2521 gccgcaagcg tcgtcgattt ccccgatccg gtcgcgccca ccatcaggat caggccccgc
     2581 ttctccagga tgatgtcctt gagcacgggc ggcaggccga gcgagtcgag cggcgggatc
     2641 gtgttgggaa tgtagcgcac caccaccgcg ggggagcctt tctggcggaa ggcgctgatt
     2701 cgaaagctgc cgatgccctg taaagtgtat gcggtgttca gttcgagcgt gtcttcgtat
     2761 tccttcctct gcttgtcgtc gatcacttca tagagcaggc cgcggaccgt gttgccgtcc
     2821 atggccttct ggttgatcgg catggcgacg ccgttgatct tgatattgat gggggagccc
     2881 accgacagga agatgtcgga ggccttcttc tcggccatca gctggaacag tcgctgcatc
     2941 gcggacatcg gcatcccctt gaggcgcggc ggctcgctac tcgccgcgca acagttcgtt
     3001 gatgctggtc ttcgcgcggg tcttggcgtc gacctgcttg actatcaccg cacagtatag
     3061 gccgtatttg ccgtcagcgg atggcagggt gccgggcacg accaccgacc cggccgggat
     3121 ccggccgtag agaatttttc cgctggcccg gtcgagaatc cgggtgctcg ctccgatgaa
     3181 tacgcccatc gagatgaccg cgttctcctc gacgatgacg ccttcgacga tttccgagcg
     3241 cgcgcccacg aagcagttgt cctcgacgat ggtgggattc gcctgcaagg gctcgaggac
     3301 cccgccgatg cccacgcccc ccgaaaggtg cacgttcttg ccgatctggg cgcacgagcc
     3361 gaccgtggcc caggtatcga ccatcgtgcc ttcgtccacg taggccccta cattcacgaa
     3421 ggaaggcatg agcacgacgt tttttgcgat gtaggctcca tggcgcgccg cggcgggagg
     3481 cacgacgcgg aatccgcctc gcctgaaacg cgcggcgtcg tagcgttcga acttgcttgc
     3541 gaccttgtcg tagaaccgca gagctccggc gcgaatcggc cggttgtcct ccagccggaa
     3601 cgacagcagc acggccttct tgatccattg gtgcgtccgc caggcaccgt cgatcttttc
     3661 cgcgacccgc agcccgcccc ggtcgagctc atcgaggacc cgagcgaccg acttgcgggt
     3721 ctttgacggc gcggagcccg gcgagaggcg ctcgcgatct tcccacgcct cctcgacggc
     3781 actctggagc ttctggttcg cgggcgagct catagtttac gggtgaattc tacgatacgc
     3841 cgagccgcct cgaggcattc gtcgatttcc gcaacgagcg ccatcctgac gtagtttgcc
     3901 ccgggattga cgccgcctga gtcgcgtgcg aggaaacttc ccggaaggac ggtgacgttc
     3961 tgttccgcgt agagcctgcg cgcgaattcg gtgtcggcga tcggcgtggg ggcccacagg
     4021 taaaagcccg catcggggcg ctcgacccgg agcgcggcct ggagcatcgg ggtgacgcgg
     4081 gcgaacttct ccgcatattt cctgcggttc tctcggacgt ggtgctcgtc gttccaggct
     4141 gcgacgctcg ctgcctgaac ggccggattc atggcgccgc cgtgataggt gcgatagagc
     4201 aagaacgctt cgaggacgga agcatcacct gcgacgaaac cggagcgcat tcccggaacg
     4261 ttggaacgct tcgagaggct cgagaacatg accaggcgct ggaaaccgcg accgagcatc
     4321 gtcgcggcct gaagggctcc gagcggcggc gcggcctcgt cgaagtagat ctccgaataa
     4381 cattcgtcgg aagcgacgat gaatccgtgg cggtccgcta tggaaaagat ctcccgccat
     4441 tcatccaggt ccaggacctt tccggtcgga ttccccggcg agcatacata gagcagttgc
     4501 gtgcggcccc agacctcttc aggaaccgca ccgtaatcgg cggcaaatcc ccgcttggat
     4561 agcgtattca cgtaatgcgc acgcgctccc gcgagcagcg cggcgccttc gtagatctgg
     4621 tagaacggat tggggcacgc gacgacggag ttcccgcgcg cagggtcgac caccgtttgg
     4681 gcaaaggcga acagggcctc gcgcgagccg ttgacgggca gcacctcggt tttccagtcg
     4741 atggcacgca ggccatagcg gcgcaccagc cattcggcga tcgcctggcg caacggatcg
     4801 ccgcccgccg tcggcgggta ggcggcgagc ttcgcgagat tttcggacag cgcgcgcttg
     4861 atcagctccg gcgttgggtg cctcggttcg ccgatgccca ggctgatgtg cgctagcccc
     4921 gggttcggct tcgcgccctc gaacagaccg cgcagtttct cgaaggggta gggctgcagc
     4981 gactgcagtc gtggattcac ggtgccagcc ctcagcgcgt gcttttctgc tcgtcgatag
     5041 tcgcgaactc caagcctgcg gcgggtctcc agcctttctt gcggtgctcg gccaccacgc
     5101 tggtgaaaat cccgccgcga acgtagaacc gcgcggcaag ccggaggtac cgcggcaaga
     5161 gcagctttac gaggtcatcg caaatgcggt tggtgaccgc ctcgtggaag gcgccctcgt
     5221 tcctgaacga ccagatctag agtttcaggc tcttcaattc cacgcatttc cggtccggga
     5281 catagtcgag gaacagcgtg gcgaagtccg gctgcccggt gagcgggcac aggcaggtga
     5341 actccgggat ctgcatccgg atcaaatagt cgcgacgtgg gctcggattg ggaaaactct
     5401 cgagggattt gctcggcttg gacggcattc agggtccggg tgcgaagttg gagatggtat
     5461 tataggggct ccgttttatc gacgatgaac ccggctgacg gccggttttc ttttacctgg
     5521 attcgtagcg tcttctgata gcgtgcggct cacacaaatc aggctcgccg ggttcaagtc
     5581 gttcgtcgat ccgtgcaccg tgagcattcc gggccaactg gtcggcgttg tcgggccgaa
     5641 cgggtgcggg aagtcgaaca tcatcgacgc cgtccggtgg gttctcggag agtcgcgcgc
     5701 ctccgctctg cgtggcgact cgatgcagga cgtgatcttc aacggctcat ccctgcgaaa
     5761 acccgtggcg cgggcgagcg tggagttgtt cttcgacaac agcctcggca gggcggccgg
     5821 ccaatggtcc cagtacgccg agatctcggt gaagcgcgtg ctgcagcggg acggcgaatc
     5881 ctcctatttc atcaacaaca ctcacgtccg ccgccgcgac gtgcaggata tttttctcgg
     5941 cacgggcctg ggtccgcgcg cctacgcgat catcgagcag ggaatgatct cgcggatcat
     6001 cgaagcaagg cccgaggaat tgcgcgtctt tctcgaggaa gcggcgggag tgtccaaata
     6061 caaggagcgg cggcgcgaaa ccgagcatcg cctcggcggc acgcgcgaca acctcgcgcg
     6121 tttgagcgac atccgccagg aactcgggtc ccagatcgag cggctcggac agcaagccga
     6181 gaccgcacgg cgctacagcg agctctcggc cgagcgtgcc cagaaacaaa acctgctgtg
     6241 gctggtgagg cgcaacgagg cgctggccga ggcgcagcgc cacacgcggg aagtcgagcg
     6301 cgctgcggtc gagctcgagg ccgaaaccgc acggctgcgc gaaagcgagc gcgatctcga
     6361 gcaggcgcgg actgagcact acgccgccgg cgacgccttg agcgcggcgc aaggcgagct
     6421 ctacggcgca aacgccgacg tttcccgcat cgagaccgag atgcgctttg tcgcggatac
     6481 gcgccagcga ctcgaagcgc agcttgcgca aatgcgcgcg caacgcgacg ccggccgacg
     6541 caacgagatc gaacagcggg aagcgctccg c
//
LOCUS       DASPZD010000163         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_342149, whole genome shotgun sequence.
ACCESSION   DASPZD010000163 DASPZD010000000
VERSION     DASPZD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342149"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VEM33_03120"
     CDS             complement(<1..353)
                     /locus_tag="VEM33_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50845.1"
                     /translation="MSEGSAASRSKLALWVGAFAGFYRPAVEEVRRLDAVSKFLYSAR
                     SVILVISAQAAIIAGLLALAARRFDALAFVLVLAGFVSAHMISNLSNDYFGYKRGHDT
                     PDSPRMRYTVHPLASG"
     gene            443..1390
                     /locus_tag="VEM33_03125"
     CDS             443..1390
                     /locus_tag="VEM33_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HYS50846.1"
                     /translation="MPGTGRQQGSRVRADARPGTVVVRALLAACCGFLFLSSGALAQS
                     PSAEDISSGKYCANCHANAHLIWAGRHGTRADARAPVSVGCAMCHGDPAEHVKSPANP
                     MPRRFSKMASAEKNEVCLTCHQGRTRIHWQGSKHERSDVSCSSCHQLHAASDPVRDKL
                     TQVDVCVACHKDIRPLINRPSRHPIREGMVSCSDCHNAHGSVGPSMVVRDSVNDTCYM
                     CHMEKRGPFVRTHQPVQEDCSICHNPHGTTAPNLLKIRQPFLCQQCHEPTSHRGTAGS
                     LTGSSSAANTVARGCLNCHTNIHGTNNPTDISNERTFRR"
     gene            1395..>3141
                     /locus_tag="VEM33_03130"
     CDS             1395..>3141
                     /locus_tag="VEM33_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HYS50847.1"
                     /translation="MKTQAALRMTGIAAAMLAAFGSARAQQVEPLAPSALNTEASSVS
                     LGVGLTPNDGRRSGEYNGITRDGAYGLIDFNLVRRDDETGTWTQVYGRNVALDNAQLR
                     FEQSRQGDWGYFVDYARIPRFEPFVITTGVTGIGTPNVTVPVTPSATGIAELSTRRER
                     LDVGGEKFFAGNWDVQLSFRNEQKNGARVFARGSTNPAFEFTPEPIDSVTRLMEAKLN
                     YTTPALQLSGGYYGTFYNNNQSNGLNIIGGNAGLASFTPIALPPDNQAHQLYLTGGYN
                     FAQSTRGNFKLAYTKATQTDAFISPVNPPQAAGTGDSLEGRVDTTLVQAGIVSSPMPK
                     LTLRGDLRYEDRDDKTPVRVFFSGISPTSTSNGENEPRSIRTSSAKAEASYALPASFR
                     LTAGIGWEEKKRNVSAVRVVSARETTDEVSYRLELRRTMTETLTGSLAYVHSDRDGSP
                     WLTTVLNDGVTPGSNLIAPIWLADRKRDKVRATAIWNPTDPLTLTLYVETAWDDYSHR
                     DNSNVGPQKGAADNYSLDVAYAFSDRWQANAWYTKYDARAEQSSGVSPVFSATLKNLS
                     DNFGLGMRGKPLEAVQ"
BASE COUNT          597 a         1111 c         1013 g          420 t
ORIGIN      
        1 ccgctcgcga gcgggtgcac cgtgtagcgc atgcgcggcg aatcgggcgt gtcgtggccg
       61 cgcttgtagc cgaaatagtc gttggagaga ttgctgatca tgtgcgcgga gacgaacccg
      121 gcgagcacca ggacgaaagc gagcgcgtcg aagcgccgcg cggcgagcgc gagcaggccg
      181 gcgatgatcg ccgcctgcgc ggaaatgacg aggatgacgc tgcgcgcgga gtagaggaac
      241 ttcgagaccg catcgaggcg cctgacctcc tcgacggccg ggcggtagaa gcccgcgaac
      301 gcgccgaccc acagcgcgag cttcgatcga cttgccgcag atccctcgct catgccattc
      361 tctccgacag cgtattgtgg ggcacagggc tgacatattg gaccttggtc caattgtgtg
      421 gcgccacggc cgagaagtta caatgcccgg caccggacgg caacagggga gcagggtgcg
      481 agctgacgca agaccgggga ctgtggtagt gcgcgcgctt ctcgccgcgt gctgcgggtt
      541 ccttttcctt tcttcgggcg ccctcgccca atccccgtcc gccgaggaca tcagcagcgg
      601 caagtactgc gccaactgtc acgccaacgc gcatctcatc tgggcgggca ggcacggcac
      661 cagggccgac gcccgggcgc ccgtcagcgt cggctgcgcc atgtgccacg gcgacccggc
      721 cgagcacgtc aagagccccg ccaatcccat gccgcggcgc ttcagcaaga tggcgtcggc
      781 ggagaagaac gaggtctgcc tgacctgcca ccagggccgc acccgcatcc attggcaggg
      841 cagcaagcac gagcgcagcg acgtttcctg ctcgtcctgc caccaactgc acgccgcgag
      901 cgacccggtg cgggacaagc tcacccaggt cgacgtgtgc gtcgcctgcc acaaggacat
      961 ccggccgctg atcaaccgtc cctcgcgcca cccgatccgc gagggcatgg tgtcgtgctc
     1021 ggactgccac aacgcgcacg gctcggtcgg cccgagcatg gtggtgcgcg acagcgtcaa
     1081 cgacacctgc tacatgtgcc acatggagaa gcgcggtccg ttcgtgcgca cccatcagcc
     1141 ggtgcaggag gactgcagca tctgccacaa ccctcacggg acgaccgccc cgaacctgct
     1201 caagatccgc caacccttcc tgtgccagca gtgccacgag ccgacgagcc accgcggcac
     1261 cgccggctcg ctcaccggct cgagctccgc cgcgaacacg gtcgcgcgcg gctgtctgaa
     1321 ctgccacacc aacatccacg gcacgaacaa cccgaccgac atcagcaacg agcggacctt
     1381 caggcgatag gaccatgaaa acgcaggctg cgctcaggat gaccggaatc gccgcggcga
     1441 tgctcgcggc gttcgggagc gcgcgcgccc agcaggtcga gccgctcgcg ccctcggcgc
     1501 tcaacaccga ggcgagctcg gtcagcctcg gggtcgggct cacaccgaac gacgggcgac
     1561 ggtccggcga gtacaacggc atcacgcgcg atggcgccta cggcctcatc gacttcaacc
     1621 tggtgaggcg cgacgacgag acggggactt ggactcaagt ctacggccgc aacgtcgcgc
     1681 tcgacaacgc gcagcttcgc ttcgagcagt ctcgccaggg ggactggggc tacttcgtcg
     1741 actacgcccg gatcccgcgc ttcgagccct tcgtgatcac gacgggagta acggggatcg
     1801 gcacgcccaa cgtgaccgtt cccgtgacgc ccagcgccac cggcatcgcc gagctcagca
     1861 cgcggcgcga gcgcctggac gtcggaggcg agaagttctt cgccggcaac tgggacgtgc
     1921 agctgagctt ccgcaacgag cagaagaacg gcgcgcgggt gttcgcccgg ggcagcacca
     1981 acccggcgtt cgaattcacc ccggagccga tcgactcggt cacccgcttg atggaggcta
     2041 agctcaacta cacgactccg gccctgcagc tctcgggggg ctactacggg accttctaca
     2101 acaacaatca atcgaacggc ctgaacatca tcggtgggaa tgccgggctc gcgagcttca
     2161 ccccgatcgc gctgccgccg gacaaccagg cccaccagct ctacctgacg ggcggctaca
     2221 acttcgcgca gagcacgcgc ggcaacttca agctcgccta caccaaggcg acgcagaccg
     2281 acgccttcat ctccccggtc aaccctcccc aggccgcggg gaccggagac agcctcgaag
     2341 gccgggtcga cacgacgctc gtccaggcgg ggatcgtctc gagccccatg cccaagctga
     2401 ccctgagggg ggacctgcgc tacgaggacc gcgacgacaa gaccccggtg cgcgtcttct
     2461 tcagcgggat ctcgccgacc tcgacctcga acggcgagaa cgagccgcgc tcgatacgga
     2521 ccagcagcgc caaggccgag gcgagctacg cgctccccgc gagcttccgg ttgaccgcag
     2581 ggatcggctg ggaggagaag aagcgcaatg tctcggccgt gcgcgtggtc agcgcccgcg
     2641 agacgaccga cgaggtctcg taccggctcg agctgcgccg gacgatgacg gaaaccctca
     2701 ccggttcgct cgcctacgtc cacagcgacc gggatgggtc accctggctg acgaccgtgc
     2761 tcaacgacgg agtcacgccc ggaagcaacc tgatcgcgcc gatctggctg gccgaccgca
     2821 agcgcgacaa agtgcgggcc acggcgatct ggaacccgac cgatccgctg accctgacgc
     2881 tctacgtcga gacggcctgg gacgactatt cgcaccgcga caactccaac gtcggcccgc
     2941 aaaagggcgc ggccgacaac tactcgctcg acgtcgcgta cgccttcagc gaccgctggc
     3001 aggccaacgc ctggtacacg aagtacgacg cgcgtgcgga gcagtcgagc ggggtgagcc
     3061 cggtcttcag cgccacgctg aaaaacctca gcgacaactt cggcctgggc atgcgcggca
     3121 agcccctgga ggcggtgcag g
//
LOCUS       DASPZD010000164         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_346314, whole genome shotgun sequence.
ACCESSION   DASPZD010000164 DASPZD010000000
VERSION     DASPZD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346314"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VEM33_03135"
     CDS             <1..599
                     /locus_tag="VEM33_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYS50848.1"
                     /translation="VLWGTAYRWVSVTDGDNGLRGLTRPTPFGISLDDAGSFYYFALA
                     ITAVAVWLMAVFVASPFGVALRGTRDQARRMSALGHDVWLIRWITFVYAAFWGAVSGL
                     LFVYYNKYIHPASLSITASAEGLLAVIAGGSATLAGPIVGAVIVMILKNYVSGFIERW
                     NMLLGFVFVFIVIFMPEGVVPGVRRLFGHGMRAWRSLR"
     gene            596..1336
                     /locus_tag="VEM33_03140"
     CDS             596..1336
                     /locus_tag="VEM33_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS50849.1"
                     /translation="MTPALQLRELRKSFGGLPAMNGVSLEVMPGERRLIIGPNGAGKT
                     TLFNLITGDLRRDSGAIRLFGDEVSRLKPHQRAHRGLARTYQIITLFSKDSLEHNLVL
                     SLLGLSKSRWNAFRPLRRYSGLNEEARRVLKRVGLEHLAQRSIGAVSYGEKRRLEIAM
                     ALAQEPRVLLLDEPFAGLSREERAAMKELIAGIPREVTVIMIEHDMDTALDLAERITV
                     LHYGSVIVEGTRAEVVADPKTREVYLGD"
     gene            1329..2042
                     /locus_tag="VEM33_03145"
     CDS             1329..2042
                     /locus_tag="VEM33_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS50850.1"
                     /translation="MTEALSLSGIDALYGESHVLHQVSFNLNAGRVLALLGRNGAGKT
                     TCMSTIIGFLPPRAGEIRLFGEAIGKLSPETISRKGVGFVPQGRRIFPSLTVQENLAV
                     SRQKRKGVAMPWTLERVIELFPRMKDRQQQLAGSLSGGEQQMLAIGRALMGNPRVLLM
                     DEPSEGLAPLIVAEVGRTLLRLKQQGQSIVLVEQNIKLALGLADEVVILNTGRVAFHG
                     PVDRVRGDQALITQHLGVF"
     gene            2067..>2569
                     /locus_tag="VEM33_03150"
     CDS             2067..>2569
                     /locus_tag="VEM33_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50851.1"
                     /translation="MMFTCVTPGCSQPGHHHPAMAAARKGGSKAARSKGHAKTVVRDR
                     KGRSLRVDIHCHYLNRDAAAKLAHLNPAQYEPSVKYASALTREVNVKQMHDRAPKLST
                     IEVRIADMDRMGIDIQAVCPAPQQTYYWTEPGLGLEVSRMINDRLAQIVATWPDRFVA
                     LGTVPLQ"
BASE COUNT          450 a          819 c          837 g          463 t
ORIGIN      
        1 aagttctgtg gggcaccgca taccgctggg tttcggtcac cgacggcgac aacggcctgc
       61 ggggattgac ccggccgacg cccttcggca tcagcctgga cgacgcgggc tctttctact
      121 attttgccct ggcgatcacc gcggtcgcgg tctggctcat ggccgtgttc gtcgcatcgc
      181 ccttcggcgt cgcgctgcgc ggaacgcgcg atcaggcgcg gcgtatgagc gcgctcgggc
      241 acgacgtctg gctgatacgc tggatcacgt tcgtctacgc cgcgttctgg ggcgctgtgt
      301 cgggactgct gttcgtgtac tacaacaagt acatccatcc cgcttcgctc tcgatcacgg
      361 cgtcggcgga gggtttgctc gcagtcatcg ccgggggttc cgccacgctc gccggcccga
      421 tcgtcggggc ggtgatcgtg atgattctga agaattacgt ttcgggcttc atcgagcgtt
      481 ggaacatgct gctagggttc gtgttcgtct tcatcgtgat ctttatgccc gagggcgtcg
      541 tgcctggagt gcgccgcctc tttggacatg ggatgagggc gtggaggagc ctccgatgac
      601 gccggccctg cagcttcgcg agctgaggaa atcgttcggc ggactgcccg ccatgaacgg
      661 cgtttcgctc gaagtgatgc ccggcgaaag gcggcttatc atcgggccga atggagcggg
      721 gaagactacg ctgttcaatc tgatcacggg ggatctgcgc cgcgactccg gtgcgatccg
      781 tttgttcggc gacgaggtgt cgcgcctgaa gcctcaccag cgcgcccatc gggggcttgc
      841 gcgcacctac cagatcatca cgcttttctc caaggattcg ctcgagcaca acctcgtgct
      901 gtcgctgctc gggctgtcca agtcgcgctg gaacgcgttc agaccgctca ggcgatactc
      961 cgggctgaac gaggaagcac ggcgggtgct gaaacgcgtc ggccttgagc accttgccca
     1021 gcgttcgatc ggcgccgttt cctacgggga gaaacgccgg ctcgaaatcg ccatggcgct
     1081 cgcgcaagag cccagggtgc tgctgctcga cgagccgttc gcggggctct cgcgcgagga
     1141 gcgcgctgcg atgaaggagc tgatcgcggg catcccgcgg gaagtgaccg tgatcatgat
     1201 cgagcacgac atggacaccg ctctcgatct cgccgagagg attaccgtgc tgcactacgg
     1261 cagcgtgatc gtcgagggta cgcgagccga agtggtcgcc gatccgaaaa cgcgcgaggt
     1321 ctacctcggt gactgaagct ctttcacttt caggcatcga cgcgctctac ggcgagagtc
     1381 atgttttgca ccaggtatcg ttcaatctga acgccggtag ggtactggcg ctgttgggcc
     1441 ggaacggtgc cggcaagacg acttgcatga gcacaatcat cggcttcctg ccgccgcgcg
     1501 caggggagat ccgcctgttc ggagaggcga tcggaaagct ctcgcccgag acgatttcac
     1561 gaaagggtgt gggcttcgtc ccgcaaggcc ggcggatctt tcccagcctg acggtgcagg
     1621 aaaatctggc cgtctcgagg cagaaacgca aaggcgtcgc aatgccgtgg accctggagc
     1681 gggtgatcga gctctttccc cgcatgaagg acaggcagca acaactggcc ggctcgctct
     1741 ccggcggcga gcagcagatg ctcgcgatcg gccgcgcgct catgggcaat ccacgcgtgc
     1801 tgctgatgga cgagccctcg gaagggctcg cgccgctgat cgtcgccgaa gtcggccgga
     1861 ccctcttgcg cctgaagcag caaggccagt cgatcgtgct ggtcgagcag aatatcaagc
     1921 tcgccctcgg cctcgcagac gaagtggtga tcctcaacac cggccgcgtc gcatttcacg
     1981 gccccgtgga ccgtgtccgc ggcgaccagg ccctgattac gcagcacctg ggagttttct
     2041 aacctgccta ccggagaagc acactcatga tgttcacctg cgtcaccccc ggctgcagcc
     2101 agccgggaca ccatcacccc gccatggccg cggcgcgcaa aggcggctcg aaagccgctc
     2161 ggtccaaagg gcatgcgaag accgtcgtgc gcgacagaaa agggcgcagc ctcagggtcg
     2221 acatccattg ccactatctg aaccgcgatg cggccgcgaa gctcgcgcac ttgaaccccg
     2281 cgcaatacga gccttcggtc aagtacgcga gcgcgctcac gcgcgaggtc aacgtcaagc
     2341 agatgcacga ccgggcgccc aagctctcca cgatcgaggt gcgcatcgcg gacatggaca
     2401 ggatgggcat cgacatccag gcggtgtgcc ccgcgccgca gcagacctac tactggaccg
     2461 agcccggcct gggcctcgag gtctcgcgca tgatcaacga ccggctggcg cagatcgtcg
     2521 cgacctggcc ggaccgcttc gtggccctcg gcaccgtgcc gctgcaaaa
//
LOCUS       DASPZD010000165         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_178954, whole genome shotgun sequence.
ACCESSION   DASPZD010000165 DASPZD010000000
VERSION     DASPZD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178954"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VEM33_03155"
     CDS             complement(<1..340)
                     /locus_tag="VEM33_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50852.1"
                     /translation="MRIDTLFAGRAVPVLIAGLVGVAAVTGWLHSGLSPKQGDARSAD
                     TGRKPAPANSCALCGTVESIRTVEVRDEAGGTGAAAGGPGGAADQAVGGGGTAATLLG
                     AGDAIAGSDIE"
     gene            complement(616..1956)
                     /locus_tag="VEM33_03160"
     CDS             complement(616..1956)
                     /locus_tag="VEM33_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYS50853.1"
                     /translation="MSTILLVDDDPDILKLISLRLTAAGHSVKGADSGEKALAVLAAS
                     RPELVITDLKMGGMDGLTLFDEIRKQAPTLPVIILTAHGTIPEAVAATRRGVFGFQSK
                     PFDGKHLLDQVEQALSLSGVARSEGEEDWRREFVTQSPKMENVLHQARLVAQSEASVF
                     IQGASGTGKELLARAIHRASRRSQGPFVAVNCAAIPENLLESELFGHRKGSFTGATYD
                     HKGLVPAADGGTLFLDEIGDMPLALQAKLLRVLQDREVRAVGATQATAVDVRVISATH
                     RDLDAQMKMGRFREDLYYRLNVVSLALPPLSERREDIVPLATHYLRVTAARYGKDVQA
                     VAPEGLQTLLAAPWPGNVRQLVNVIEQAVALCPSGVVPASLIQQALKEEPAQLASLEE
                     ARGAFEREYLVRILRITGGSVTRAAKLAQRNRTEFYKLLERHKLEPKMFKPEKG"
     gene            complement(1953..>2139)
                     /locus_tag="VEM33_03165"
     CDS             complement(1953..>2139)
                     /locus_tag="VEM33_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50854.1"
                     /translation="LAGVGASESASGPMQQFAGLLHAQISERLREQRRTVQLKEQLEA
                     LKAVERNIMEREQARPR"
BASE COUNT          351 a          718 c          677 g          393 t
ORIGIN      
        1 tctcgatatc gctcccggcg atggcgtctc ccgccccgag aagcgtggcc gcggtgccac
       61 ctccccctac tgcctggtcg gcggcgccgc cggggccgcc tgccgccgcc ccggtaccgc
      121 ccgcttcatc gcgcacctcg acggtccgga tggactcgac cgttccgcac aaggcacagc
      181 tatttgccgg ggcgggtttg cgcccggtgt ctgccgaccg cgcgtcgccc tgcttgggcg
      241 agaggccgct gtgcaaccag cccgtgacgg ctgcaacgcc gacaagaccc gcaatcagga
      301 ccggaaccgc ccgccctgcg aagagagtat cgatccgcat ctatccgtat tggagcgcgc
      361 tcgcttcagc aaatttgttc ttcccccgcc agcatcgcgg cggaatcgct ggcatgtttg
      421 caatccccgt gccagcatag gttctcgcgc taactcaacg agttatcggg aatccaggat
      481 cccgaggact ttcaatgcgt cgcttgtcaa cgacgctttc ccgcaagcgg caaaaatcga
      541 actgatggga aagggaattt gttgtcgccc cggggcgaca acgccacgaa ttccgctggc
      601 gcggatcggc cggcttcagc ccttttcggg cttgaacatc ttcggctcga gcttgtggcg
      661 ctcgagcagc ttgtagaact cggtgcgatt gcgctgcgcg agcttcgccg cccgggttac
      721 gctgccaccc gtgatgcgca ggatccgaac caggtattcg cgctcgaagg ctcctcgcgc
      781 ctcctcgagc gacgcaagct gcgcgggctc ctccttcaac gcctgctgga tcagcgaggc
      841 gggcacaacg cccgaagggc agagcgcaac ggcctgttcg atcacgttga cgagctgccg
      901 cacgttgccc ggccaaggtg ccgcgaggag cgtttgaagc ccctccggag ctactgcctg
      961 cacgtccttt ccgtaacgcg cggcggtgac tctgaggtaa tgggttgcca gagggacgat
     1021 gtcctcgcgc cgctcggaga ggggtgggag cgcgagcgag accacgttca gtctatagta
     1081 gagatcctcg cggaaccgtc ccattttcat ctgcgcgtcg aggtcgcggt gagtggcgga
     1141 gatgacgcgc acatcgactg ccgttgcctg agtcgcgccg accgcacgca cttcgcggtc
     1201 ctgcagcacg cgcaggagct tggcctgcaa ggcaagaggc atgtccccga tttcatccag
     1261 gaacagcgtc cctccgtcgg cggcggggac gagccccttg tgatcgtagg tcgcaccggt
     1321 aaacgagccc ttgcggtggc cgaacaattc cgattcgagc agattttccg ggatggccgc
     1381 gcaattgacc gcaacgaagg gtccctgcga gcggcggctc gcccggtgta tcgcgcgcgc
     1441 gaggagctcc tttcccgtgc cgctcgcgcc ctggatgaac acgctcgcct ccgactgggc
     1501 aacaaggcgc gcctgatgga gcacgttctc cattttcggg ctttgcgtca cgaactcgcg
     1561 ccgccagtcc tcctcgccct cactccgtgc gactccggaa aggctcaggg cctgttccac
     1621 ctggtcgagc aggtgcttcc cgtcgaacgg cttggactga aacccgaaaa ccccgcggcg
     1681 ggtcgcggcg accgcctcgg ggatggtccc atgcgcggtc agaatgatga ccggaagggt
     1741 cggcgcctgc ttgcggatct cgtcgaacaa ggtcaagccg tccattccgc ccatcttgag
     1801 gtcggtgatg acaagctcag gtcgcgaagc tgcgagcacg gcgagcgcct tctccccgct
     1861 gtccgcgccc ttgacgctgt ggccggcggc ggtaagtcgc agggaaatga gcttcaggat
     1921 atccggatcg tcgtcgacga ggagaatggt gctcatcttg gccgcgcttg ctcgcgttcc
     1981 atgatattgc gctcgaccgc cttcagcgcc tcgagttgct ccttcagttg gaccgtgcgg
     2041 cgctgctcgc gcaagcgttc gctgatctgg gcatgaagca gcccggcaaa ctgctgcatc
     2101 gggcccgacg ccgattcgga ggctcccacg cccgcgaga
//
LOCUS       DASPZD010000166         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1736978, whole genome shotgun sequence.
ACCESSION   DASPZD010000166 DASPZD010000000
VERSION     DASPZD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736978"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..129
                     /locus_tag="VEM33_03170"
     CDS             1..129
                     /locus_tag="VEM33_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYS50855.1"
                     /translation="MARELRLSPKTASTYKTRIMQKLNLQSDDDLIRYALANHLVK"
     gene            291..704
                     /locus_tag="VEM33_03175"
     CDS             291..704
                     /locus_tag="VEM33_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYS50856.1"
                     /translation="MEKTIDGASGSTRPSILVVEDHRSLRLSLVIWLQCLFPGSLVQG
                     VRSGEEALAHAGNAPPDVVLMDINLPGIDGIEATYRMKALAQTTAVVVLSAHDTPHHR
                     LVAEKAGAAGYIAKADMEKQLEPTVRGLLRSRTRS"
     gene            701..1468
                     /locus_tag="VEM33_03180"
     CDS             701..1468
                     /locus_tag="VEM33_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYS50857.1"
                     /translation="MTRGLDMGTRLRRALGHGELELHYQPQVGFASGEINSVEALLRW
                     NNPELGGVAPAQFIPLAEETGLIQPIGAWVLETACAQARAWQDAGLKPIKMCVNVSAR
                     QLDGKLVGTVSRVLAETAIAPSRLELEITEGVMVGKDPGTEAVLQAMRALGVGFAIDD
                     FGTGYATFDYLKRFPVHTLKLDGSFVRGVCDSRNDVAIVDATIALARSFGLRVVAEGV
                     ETAEQHALLGELGCDDCQGYYTVRPLPAAALEKLLAP"
     gene            1612..1998
                     /locus_tag="VEM33_03185"
     CDS             1612..1998
                     /locus_tag="VEM33_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50858.1"
                     /translation="MNFTIRYDGDLLRAHVWGRETDNPPSHICRLILKECRRHGLKRI
                     LIELNQERPLSDASQYLLVERLPALGLTLEHRIALVHHTPGLYEANDMIDLVAKNRGI
                     NVRNFRDVRSATEWLHSPASPAETLQ"
     gene            2178..3260
                     /locus_tag="VEM33_03190"
     CDS             2178..3260
                     /locus_tag="VEM33_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HYS50859.1"
                     /translation="MPHRRSTMHAMFIAAFALAASTSVLAQQKGGKHHTLAATLETVQ
                     WGWLDPNEPPKLKINSGDTVSIETMMHAHDKVKPGATMDEIVALRKANPGGGPHSMTG
                     PIYVEGAEPGDVLEVRILKIVPKPFGVNFNLPGKDFPTIGALAPEMPDGFVRFMSLDL
                     KTRTAEFKPGITIDLKPFPGTLAVGIDPNDPSPRKGGVKDNPMAPVSTLRPWKNGSNM
                     DINELQEGSTIFFPVFLKGGLIWTGDSHCRQGNGEVNLTALECSFKEIRIQPIVRKDM
                     KLEWPRIETRTHWISVGFDEDLNKAMVNAVREAVDFLAAQKAVPLTRYEAYSLVSMVG
                     DCRVSQVVDGRKGVHCMIPKSVFTKK"
     gene            complement(3318..3737)
                     /locus_tag="VEM33_03195"
     CDS             complement(3318..3737)
                     /locus_tag="VEM33_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYS50860.1"
                     /translation="MYASIPAHTPRSPPMNSLVALAGRFLLALIFLLSGIDKVVHYAG
                     TLGYMTKAGLPFPQVLLIVSIIVEIGAALAIIAGWKTRWAAAALVLWMIPVTLVFHNP
                     AGGQEQMAHFMKNVAIVGGLLILWALGPGALSLKRSS"
     gene            3765..>4050
                     /locus_tag="VEM33_03200"
     CDS             3765..>4050
                     /locus_tag="VEM33_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HYS50861.1"
                     /translation="MSASPREIVRNNVKEKLARGEVVSSITVRLVRSIEIARIAKTAG
                     FDSIYVDVEHSSLSLETTSQICIAALEIGVAPFVRVPSTRPEHVSRALDGG"
BASE COUNT          788 a         1332 c         1264 g          666 t
ORIGIN      
        1 atcgcgaggg agctccgcct cagcccgaag acggcgagca cctacaaaac gagaatcatg
       61 cagaagctca atctgcaatc cgacgacgat ctcatccgct acgccctcgc gaaccacctc
      121 gtgaagtagc cgggccgcag cccgggtgcc gcgccctccc ggttcgggac gtttgccgcg
      181 cggccacggc gaaatgtagg aattccccta cacgctgctg cgccgattcc cacagggaag
      241 taggcgcgcg ctgatttttt tcccttccgc ccccggtcac aatgcgcgtc atggaaaaaa
      301 cgattgacgg tgcatccggc tcgacccgcc cttcgatcct ggtcgtcgaa gaccatcgat
      361 ccctgaggct cagcctcgtg atctggcttc aatgcctgtt ccccgggagc ctcgtccaag
      421 gcgttcgatc cggcgaggaa gcgcttgcgc atgccggtaa cgcccccccc gacgtcgtgc
      481 tcatggacat caaccttccg gggatcgacg gaatcgaggc aacttaccgc atgaaggcgc
      541 tggcgcagac cacggccgtg gtggtgctca gcgcgcacga cactccccat caccgcctcg
      601 tggccgagaa agccggcgcc gcagggtaca tcgcgaaggc cgacatggaa aaacagctcg
      661 agccgacggt ccgagggctc cttcgatcgc gcactcgctc atgacgcgcg ggctcgacat
      721 ggggacccgg ctgcgccgcg cgctcgggca cggcgagctc gagcttcact atcagccgca
      781 agtcggcttc gccagcggcg aaatcaattc ggtcgaggcc ctgctccgct ggaacaatcc
      841 ggagctgggc ggggttgcgc cggcgcagtt cataccgctc gccgaagaga ccgggctcat
      901 tcagccgatc ggcgcctggg tattggagac tgcctgcgcg caggccaggg cctggcagga
      961 cgccgggctc aagcccatca agatgtgcgt caacgtgtcc gcgcggcagc tcgacgggaa
     1021 gctcgtcggg accgtttccc gggtgctcgc cgagacggcg attgcgcctt cgcgcctcga
     1081 gctggaaatc acagagggtg tgatggtcgg caaggacccc ggcaccgagg ccgtgctcca
     1141 ggcgatgcgc gccctgggcg tcggtttcgc catcgacgac ttcggcaccg gctatgcgac
     1201 cttcgattat ctcaagcgct ttcccgtgca cacgctgaaa ctggacggct cgttcgttcg
     1261 cggcgtatgc gacagccgca acgacgtggc gatcgtcgac gcgaccatcg cgctggcgcg
     1321 aagcttcggc ctcagggtcg tcgcagaggg cgtcgaaacc gccgagcagc atgcgctgct
     1381 cggggaactg ggttgcgacg actgccaggg ctattacacc gtccggccgc tcccggctgc
     1441 ggcgctggaa aaactgcttg ccccttagca tcaagctttc cctgcaaacc ggcaaacaaa
     1501 ctgaaggagt cttcatgcca gtcaagaaaa tcctgatcgt cgacgattcg ccgaccgcga
     1561 gcagccggat ctcattctga tggacgtcgt cgagaaaatc gcggccttgc catgaacttt
     1621 acgatccgct acgacggcga tctcttgcgg gcccacgtat ggggcagaga aaccgacaat
     1681 cctccgtctc acatttgcag attgatcctc aaggaatgcc gtaggcacgg gctgaaacgc
     1741 atcctgatcg agctgaatca ggagcggccg ctctccgatg cgagccagta ccttctggtg
     1801 gaaaggctgc ccgcgctcgg gctcacgctc gagcaccgga tcgctctcgt ccaccacacg
     1861 cccggactct acgaggcgaa cgacatgatc gatctcgtcg ccaagaaccg cgggatcaac
     1921 gtgcgcaatt ttcgggacgt gcgctcggcc accgagtggc tgcactcgcc cgcttcccct
     1981 gcggagaccc tccaatgatc cgtcgcatgc tgctcatggt caggaatcgc cggcaatttc
     2041 gcatgagctg gcgcgccgcc tggatctttg ccgccgggcg atgaggtccc aggtcaagcc
     2101 gccgtgcagc gcggggcttg acgtccccgc gggccgggcc gaggatacgc cctcaaacaa
     2161 accacagagg ggatcccatg ccacaccgcc gttccacgat gcatgcgatg ttcatcgccg
     2221 cgttcgcgct cgcggcaagc acgtcggttt tagcgcaaca aaaaggcggc aagcaccaca
     2281 cgctcgccgc gacgctcgag acggtgcagt ggggctggct cgatccgaac gaaccgccca
     2341 agctcaagat caactccggc gacacggtct cgatcgagac catgatgcac gcacacgaca
     2401 aggtgaaacc cggcgccacg atggacgaga tcgtcgcgct gcgcaaggcc aatcccggcg
     2461 gcgggccgca ttcgatgacc gggcccatct acgtcgaggg cgccgagccc ggcgacgtgc
     2521 tcgaagtgcg catcctcaaa atcgtgccca agcccttcgg ggtcaacttc aatcttccgg
     2581 gcaaggattt cccgaccatc ggcgcgctcg cgccggagat gcccgacggc ttcgtgcgtt
     2641 tcatgtcgct cgacctgaaa acacgcaccg ccgagttcaa gcccgggatc accatcgacc
     2701 tgaaaccctt tccgggcacg ctcgcggtcg gcatcgaccc caacgatcct tcgccccgca
     2761 agggcggcgt gaaggacaac ccgatggcgc cggtgtcgac gctgcgcccg tggaagaacg
     2821 gctcgaacat ggacatcaac gagcttcagg aagggtcgac catcttcttc ccggtgttcc
     2881 tgaaaggcgg gctcatctgg accggcgact cgcactgtcg ccagggcaac ggcgaggtca
     2941 acctcaccgc cctggagtgc tccttcaagg aaatcaggat tcagccgatc gtgcgcaagg
     3001 acatgaagct cgagtggcct cgcatcgaga cgcggaccca ctggatctcg gtcggattcg
     3061 acgaagacct caacaaggcg atggtgaacg cggtgcgcga ggcggtggat ttcctcgcgg
     3121 cgcagaaggc tgtgccgctc acgcgctacg aggcctactc gctagtctcg atggtgggcg
     3181 actgccgcgt ctcgcaggtc gtggacgggc gcaagggcgt gcactgcatg atcccgaagt
     3241 cggtgttcac gaagaaataa gtcaaagggc gggcgccttt ttcttcggca ggcgccgacg
     3301 tgaggcagcc gttccgccta gcttgagcgc ttgaggctca aggcgcccgg accgagtgcc
     3361 cagaggatga ggagcccgcc cacgatggcg acgttcttca tgaaatgggc catctgttcc
     3421 tggccacccg cgggattgtg aaaaaccaga gtgaccggaa tcatccagag aaccagcgcc
     3481 gccgccgccc agcgcgtctt ccagccggcg atgatcgcga gcgccgcccc gatctcaacg
     3541 atgatagaaa caatcagcag cacttgaggg aacggcaggc cagccttggt catgtacccc
     3601 agggtaccgg cgtagtgcac gaccttgtcg attccagaca gcaaaaagat cagcgcgaga
     3661 aggaagcggc cggcgagggc gacgaggctg ttcatgggcg ggctccttgg agtgtgagcg
     3721 ggaattgaag catacaataa cgggccatcg cccgggagaa cgccatgtcc gcatcgccgc
     3781 gggaaatcgt gcgcaacaac gtcaaggaga agctcgcgcg cggcgaggtc gtctcgtcga
     3841 tcacggtgcg cctggtgcgt tcgatcgaga tcgcgcgcat cgcgaagacc gccggcttcg
     3901 attccatcta tgtggacgtc gagcattcga gcctgtctct ggagacgacc agccagatct
     3961 gcatcgccgc gctcgagatc ggcgtcgcgc ccttcgtgcg cgtgccgagc acccggcccg
     4021 agcacgtgtc gcgggcgctc gacggcggcg
//
LOCUS       DASPZD010000167         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1455083, whole genome shotgun sequence.
ACCESSION   DASPZD010000167 DASPZD010000000
VERSION     DASPZD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455083"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VEM33_03205"
     CDS             complement(<1..441)
                     /locus_tag="VEM33_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HYS50862.1"
                     /translation="MKQTLQLKLSQHLTLTPQLQQSIRLLQLSTMELNQELEKFLAEN
                     PLLEREDTDNDVPAVPAQMNGEPAPALQAPAEEPSTGDSLQTDTFSAEVDWGGEGGSS
                     SGARDDSDDNDFFQVAAESPSLREHLYRQLTLTQLEPGDRNLVAF"
     gene            complement(531..1253)
                     /gene="lptB"
                     /locus_tag="VEM33_03210"
     CDS             complement(531..1253)
                     /gene="lptB"
                     /locus_tag="VEM33_03210"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HYS50863.1"
                     /translation="MSQLQVHGLKKHFGSRVVVKDVSLEVSSGEVVGLLGPNGAGKTT
                     CFYMIVGLVAVDDGAIHLDGKEISHFAMHRRARMGLSYLPQEASVFRKLTVEENIRAV
                     LELQDLPEEEIESRLSGLLRDLNIAHLRQSTALSLSGGERRRVEIARALATRPSFILL
                     DEPFAGVDPIAVLDIQKIIRFLKERGIGILITDHNVRETLGICDHAYIINDGLVLAFG
                     KPDEIVYNENVRRVYLGENFRL"
     gene            complement(1288..1860)
                     /gene="lptA"
                     /locus_tag="VEM33_03215"
     CDS             complement(1288..1860)
                     /gene="lptA"
                     /locus_tag="VEM33_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HYS50864.1"
                     /translation="MRRPGNKAGAAAALGLAAAWALAAGSALAERADLEKPVNIESDS
                     MIADEAKKIATFEGKVVLTQGSLIIRADRIVVRQDSDGFYNGVATGSPATFRHKREAS
                     GDYIDGEALKIEYDNKLDRVEFSNSARLRRDSGDDIRGDYISYDAKTERFAVKSTSAE
                     SGTERVRATIMPKKPAAAPSPPVSGAGRQD"
     gene            complement(1841..2419)
                     /gene="lptC"
                     /locus_tag="VEM33_03220"
     CDS             complement(1841..2419)
                     /gene="lptC"
                     /locus_tag="VEM33_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HYS50865.1"
                     /translation="MKTRVTPLLPLVLMAFLGLLTLWLQYAVLEGSGGDAKPARHDPD
                     AIVENFTVQRLDDSGRLRYTFSAPKMTHFSDDGSGEVLYPRLVQIAEDGGNFSATANR
                     GTVTRQGEEAFLYGNVVILREATPQRPEFRARTEFLHVLAEQGISRTDQTVTISEGRS
                     ILSGVGMVVNNNKQQFTLQSQVKATFDAPARK"
     gene            complement(2416..2943)
                     /locus_tag="VEM33_03225"
     CDS             complement(2416..2943)
                     /locus_tag="VEM33_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit delta"
                     /protein_id="HYS50866.1"
                     /translation="MRADILERIRRLRLMIFDVDGVLTDGTLYFSETGAELKAFNARD
                     GHGLKMLKESGVEVAILSARRSRAVEVRAAELGITLVEQGAGDKSTAFESLLGQAHTS
                     ASAAGYMGDDWVDLPVLARCGFSASVPEAPELVRERVHHVTRAPGGRGAAREVCELIM
                     QAQDTLERALARQLA"
     gene            complement(2943..3935)
                     /locus_tag="VEM33_03230"
     CDS             complement(2943..3935)
                     /locus_tag="VEM33_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HYS50867.1"
                     /translation="MNDRSKKVISTSALELARKVLAIEADAVSGLISRLDERFVDAVG
                     LILACRGRVVVSGVGKSGHVARKIASTLASTGTPAFFVHAAEAGHGDLGMITRDDVVL
                     ALSNSGQSDELLTIIPLIKRRGAKLIALTGNPDSALAREADVYLDAHVAQEACPLNLA
                     PTASTTAALALGDALAVALLDARGFDERDFARAHPGGALGRKLLTHVSEVMRTGDDVP
                     AVYDTDSFSDMLAEISRKSMGMTAILDRGKKVVGIFTDGDLRRTLESKPDIRGLKTAD
                     VMKRNPRTISPHKLAAEALELMERYKINQLLVATETGELAGALNMHDLFRAKVI"
BASE COUNT          685 a         1250 c         1284 g          775 t
ORIGIN      
        1 aaacgcgacc aggttgcggt cgcccggttc gagctgggtc aaggtgagtt gccggtacag
       61 gtgctcgcgc aggctgggag actcggcggc aacctggaaa aaatcgttgt catcggagtc
      121 gtcgcgggcg cccgagctcg aaccgccttc gccgccccaa tcgacctcgg ccgaaaacgt
      181 gtcggtctgc agtgagtccc cggtgctagg ctcctcggca ggcgcctgaa gggcgggcgc
      241 aggctcgcca ttcatttgcg cgggtaccgc gggaacgtcg ttatcggtgt cttcgcgctc
      301 gagcagcgga ttttcggcca ggaatttctc cagctcctga ttcaattcca tggtcgagag
      361 ctgcagcagg cgtatcgact gctgcagctg cggcgtcagc gtcaggtgct gcgaaagctt
      421 gagctgtaga gtctgcttca tcgagatggg gcgtgaggac ctcgccggga gatcgtaatt
      481 cagacgggtc gaaatgggaa cggggggtgg ttcgcgggat gcgttgtccc ctacaacctg
      541 aaattttcgc cgagataaac gcgtcttacg ttttcattat agacgatctc gtcgggtttg
      601 ccgaaggcga gcaccaatcc gtcgttgatg atgtaggcgt ggtcgcaaat ccccagcgtt
      661 tcccgcacgt tgtggtccgt gatgaggatg ccgatgccgc gttccttgag aaagcggatg
      721 attttctgaa tgtcgagtac cgcgatcgga tcgacgcccg cgaagggctc gtcgagcaat
      781 atgaagctcg ggcgcgtcgc gagggcccgg gcgatctcga cgcggcggcg ctccccgccc
      841 gacagcgaca gcgcggtcga ttgccgcaga tgcgcgatat tgagatcgcg cagcagcccg
      901 gaaagacggg actcgatctc ctcttcggga aggtcctgca gttcgagaac cgcccggatg
      961 ttctcctcga cggtcagctt gcggaacacg gaagcttcct ggggcaggta cgacagcccc
     1021 atgcgggcgc ggcggtgcat cgcgaaatgg ctgatttcct tgccgtcgag gtggatcgct
     1081 ccgtcgtcca cggcgaccaa gcccacgatc atgtagaaac aagtcgtctt gcccgcgccg
     1141 ttcggaccga gcagtcccac gacctcgccg ctcgatacct cgagcgacac gtccttgacc
     1201 accactcgcg agccgaagtg ctttttgagt ccgtggacct gcagctggct catcgagttt
     1261 cgccccgttc ggaagggtcc ggggggacta gtcctgccgg cccgcgccgg aaacaggcgg
     1321 actcggcgcc gccgcgggtt tcttcggcat gatcgtggcg cgcacgcgct cggtgccgga
     1381 ctccgcactg gtcgatttca cggcaaagcg ttcggttttg gcgtcatagg agatgtagtc
     1441 gccgcgaata tcgtccccgc tgtcgcgacg caaccgggcg ctgttggaga attcgacgcg
     1501 atcgagcttg ttgtcgtatt cgatcttcag agcttccccg tcgatgtaat ctccgctcgc
     1561 ttcccgtttg tggcggaagg tcgccggact gccggtcgcg acgccgttgt agaagccgtc
     1621 gctgtcctgt cgtacgacga tccggtctgc acgaatgatg agcgagcctt gggtcagcac
     1681 gacctttccc tcgaaggtcg cgattttctt ggcttcgtcg gcaatcatgc tgtcggattc
     1741 gatgttgacg ggcttttcga gatcggctcg ctcggcaaga gccgatcccg ccgccaacgc
     1801 ccacgctgcc gcaagaccca gggccgcggc ggcgcccgcc ttatttcctg gccggcgcat
     1861 cgaaggtggc cttgacttgg gactgcagcg tgaattgctg tttgttgtta ttcaccacca
     1921 tgccgacccc gctcagaatc gaacgtcctt ccgaaatcgt gactgtctgg tcggtacgcg
     1981 aaatcccctg ctccgcgagc acgtgcagga attcggtgcg cgcgcgaaat tcaggccgct
     2041 gcggcgtggc ctcgcgcaag atcaccacat tgccgtaaag gaacgcctcc tcgccttgtc
     2101 gagttacggt gccgcgattc gccgttgcgg agaaattgcc gccgtcctcg gcaatttgaa
     2161 cgaggcgtgg atagagcact tcgcccgaac cgtcgtcgga gaaatgtgtc atcttcggcg
     2221 cggagaatgt gtaccggagc ctgcccgaat cgtcgagcct ctggacggtg aagttttcga
     2281 cgatcgcatc gggatcgtgc ctggcgggct ttgcgtcccc gccggagccc tcgagcacgg
     2341 catattgcag ccacagggtc agcaacccga gaaacgccat caaaacgagc gggagcagag
     2401 gagtgacgcg cgttttcatg caagctggcg ggccagggcc cgctccagcg tgtcttgggc
     2461 ctgcatgatc agctcgcaca cctcgcgggc ggctcctctg ccgccgggag cgcgcgtgac
     2521 gtggtggacg cgctcgcgca cgagttccgg cgcttcgggc acgctcgctg aaaaaccgca
     2581 acgcgcgagc accggcaggt ccacccagtc atcgcccatg tacccggcgg cggacgcgga
     2641 ggtgtgcgcc tggccgagca ggctttcgaa agcggtactc ttgtccccgg ccccttgttc
     2701 gacgagcgtg atgccgagct ccgcggcgcg cacttcgacg gcgcgcgagc gacgcgcgga
     2761 aagtatggcg acctcgaccc cgctttcctt cagcatcttc agcccgtggc cgtcccgcgc
     2821 gttgaatgcc ttcaactccg ctccggtctc gctgaagtac agggtcccgt cggtgagcac
     2881 gccgtcgaca tcgaagatca tcaacctcag ccgccgtatc cgctccagaa tgtcggcgcg
     2941 catcaaatga ccttggcgcg aaacagatcg tgcatgttga gggcgccggc cagttctccc
     3001 gtttccgtgg cgacgagcag ctgattgatc ttgtaacgct ccatcaattc gagcgcttcc
     3061 gcggcgagct tgtgcgggga aatcgtgcgc ggattgcgct tcatgacgtc ggcggtcttg
     3121 agcccgcgga tgtcgggttt gctctcgagc gtccgccgca gatcgccgtc ggtgaatatt
     3181 ccgacgacct ttttcccgcg gtcgaggatc gcggtcattc ccatgctctt cctggatatc
     3241 tcggccagca tgtcggagaa ggaatccgtg tcgtatacgg ccgggacgtc gtcgccggtg
     3301 cgcatgacct cggagacatg agtgagaagc ttcctgccga gcgcgccgcc ggggtgagcg
     3361 cgggcaaaat cccgctcatc gaaaccgcgc gcgtccagca aggcgaccgc aagcgcgtcg
     3421 ccgagcgcaa gcgccgccgt ggtgctcgcc gtcggcgcga gattcagggg gcaggcttcc
     3481 tgcgcgacgt gcgcgtcgag gtacacgtct gcttccctgg caagggccga gtcgggattg
     3541 ccggtcaggg cgatcagctt cgcaccccgg cgcttgatca gagggatgat cgtgagcagc
     3601 tcgtccgact gcccggaatt cgagagcgcc agcaccacgt cgtcgcgtgt gatcatgccc
     3661 agatcgccgt gtcccgcctc ggcggcgtgc acgaagaaag ccggtgtccc ggtgctcgcc
     3721 agcgtcgagg cgatcttgcg cgcgacgtgc cccgacttgc cgacaccgct cacgacgacg
     3781 cggccgcgac acgcgaggat caggccgact gcatcgacaa aacgctcatc gagccgcgat
     3841 atcaggccgc tcaccgcatc ggcttcgatc gcgagcacct tgcgcgcgag ctcgagtgcc
     3901 gaggtcgaga tcaccttctt cgatcgatcg ttcattgaag aaaacgacgc tggcctgcgc
     3961 cgggagcagc ctttatcata ggctggagag tata
//
LOCUS       DASPZD010000168         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1694676, whole genome shotgun sequence.
ACCESSION   DASPZD010000168 DASPZD010000000
VERSION     DASPZD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694676"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..206)
                     /locus_tag="VEM33_03235"
     CDS             complement(3..206)
                     /locus_tag="VEM33_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50868.1"
                     /translation="MADPLPSFLASPSPPKLKLPPGACDAHVHVFGPHGMLVDLLAEI
                     APSEKQRQALLVDNPRCFYRFRR"
     gene            complement(215..1177)
                     /locus_tag="VEM33_03240"
     CDS             complement(215..1177)
                     /locus_tag="VEM33_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50869.1"
                     /translation="MIAAIALAAAQGLLPGAALGQSYPAKPVRLIVGFPAGGPADIFG
                     RTFAQGLGTGLGQTVIVENKSGVGGVLGIDAVAKASPDGYTLGFNNQGSVSMAPYALS
                     KMPFNPNKDLAFITTVVKVPEVVVVNPSLPVNSLAELIAYAKANPGKINFGSAGAGGI
                     THLACELLKAEAHVDLLHVPYKGAAPAVSDLLGGQVNMGIFDIPVVLPHIQSGKFKAL
                     AVTSARRAPSLPNVPTTAEVGYPKVISDNWYGLVAPAATPPAVLKRIHGAAVAALRSP
                     ALLDQFAKVSGIAFPSTPEEYAAFLAEEQARWSAIIKAIGFKED"
     gene            1293..1976
                     /locus_tag="VEM33_03245"
     CDS             1293..1976
                     /locus_tag="VEM33_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYS50870.1"
                     /translation="MNGQSRRECAAMLLALGMLIAPHAAAQPADASSSNVGRFYAFGG
                     LAVFAPDKDGQLRNQQGNLANVIAGGGLRASQLFSIEAGLLAFGQKLDTPAATTPPGG
                     TFQPGSLSTRMGTLGVNLTAKLQFAMDRLEPYFGAGVGIYSTRIRTTSEAVGCAQNCG
                     DTGPRITANSRDAGYHALVGADYHITQKDVVGVEFRQLWLDASFDEVGMGKVKAGGSL
                     LWMGYRRYF"
     gene            complement(2025..2309)
                     /locus_tag="VEM33_03250"
     CDS             complement(2025..2309)
                     /locus_tag="VEM33_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50871.1"
                     /translation="MTSLSLRESVLAFMTSNPTAWRIKALAAKLGVGVQRMGLELSQL
                     NAEGKLVSCTVKAPGRRPEEEYRIAATEQKIDPHHFVISRRTNVRLRGAG"
     gene            complement(2321..2581)
                     /locus_tag="VEM33_03255"
     CDS             complement(2321..2581)
                     /locus_tag="VEM33_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50872.1"
                     /translation="MASPTQPAASAGVQDLASRIYVELVGRAFLRAENAAVFKPNPAE
                     LAKLSLQLADTFRKVEKAASAAAGPQNVGYNVDLADMAKWDK"
     gene            2676..>2941
                     /locus_tag="VEM33_03260"
     CDS             2676..>2941
                     /locus_tag="VEM33_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HYS50873.1"
                     /translation="MDTESKWKHRGVRVVRANELDINTAQTPGMNRAAAITNARAGAE
                     KLWAGTVIIHPKAKTGAHHHGPIESVIYVVSGRARMRWGDRLEFV"
BASE COUNT          520 a          954 c          983 g          484 t
ORIGIN      
        1 cgtcatcgcc gaaaccggta gaagcaccgc ggattgtcga ccagaagcgc ctggcgctgc
       61 ttctccgagg gcgcgatctc cgcgagcagg tcgaccagca ttccgtgcgg cccgaacacg
      121 tgcacgtgcg cgtcgcacgc gcccgggggc agcttgagct tcggcgggct aggcgaagcg
      181 agaaaggacg gcaggggatc tgccatttgc tgcgctagtc ttccttgaag ccgatcgcct
      241 tgatgatcgc gctccatctc gcctgctcct cggcgaggaa ggctgcgtac tcttccggcg
      301 tcgacgggaa ggcgatcccg ctcaccttgg cgaactgatc gaggagcgcg ggggagcgca
      361 gcgccgcgac agccgcgccg tgaatccgct tgaggacggc gggcggcgtc gccgccggcg
      421 ccaccaatcc gtaccagttg tcggagatca ccttcgggta acccacttcg gcggtggtcg
      481 ggacattggg caaggacggc gcgcgcctcg cgctcgtcac cgcaagcgcc ttgaacttgc
      541 ccgactggat gtgcgggagc accacaggaa tgtcgaagat ccccatgttg acctgcccgc
      601 ccagcaagtc gctcaccgcg ggcgcagccc ccttgtaggg cacgtgcagg agatccacgt
      661 gcgcctcggc cttgagcagc tcgcaggcga gatgcgtgat gcctcccgcg ccggcggagc
      721 cgaagttgat cttgccggga ttcgccttgg cgtaggcgat cagctcggcg aggctattca
      781 cggggagcga aggattcacc accacgacct cgggaacctt cacgaccgtg gtgatgaacg
      841 cgagatcctt gttcgggttg aagggcatct tggagagcgc gtacggcgcc atcgataccg
      901 agccctgatt gttgaagccg agggtgtagc cgtcgggcga ggccttggcc accgcgtcga
      961 tgccgagcac gcctcccacg ccgctcttgt tttcgacgat gaccgtctgg ccgaggccgg
     1021 tgcccaagcc ttgagcgaag gtccgcccga aaatatccgc cggacctccg gcgggaaatc
     1081 ccacgatcaa gcgcacgggc ttggcggggt aggactgccc aagggccgcg ccgggcaaca
     1141 gaccctgggc cgcggcaagc gcaattgcgg cgatcatgct tctcatccga tgttctccca
     1201 ggtcaactca tcggctctca ttcacagtga gccgcgcaag catacaataa ggaggcgcgc
     1261 gcctcgcatg ggcgcttaag cgggggtcgg aaatgaatgg ccaatccaga cgtgaatgcg
     1321 ccgcgatgct gctcgcattg gggatgctga tcgctccgca cgccgcggca caacccgccg
     1381 acgcgagctc gtcgaacgtg gggaggttct acgcctttgg cggtctggcc gtgttcgctc
     1441 ccgacaagga cggccagctg cggaaccagc agggaaatct cgccaacgtg atcgccggcg
     1501 gcgggctgcg ggcttcgcag ctcttctcca tcgaggccgg cctcctcgcc ttcggccaga
     1561 agctggacac gccggcggcg accacgcccc cgggcgggac gttccagccc gggtcgctga
     1621 gcacacgcat gggaacgctg ggagtgaacc tcaccgccaa gctgcaattc gcgatggatc
     1681 gcctcgaacc gtacttcggc gccggagtcg ggatctactc gacccggatc aggacgacga
     1741 gcgaagccgt cggctgtgcc cagaattgcg gggacaccgg tcccaggatc accgcgaact
     1801 cccgcgacgc cgggtatcac gccctcgtcg gcgcggacta ccacataacg cagaaggacg
     1861 tggtcggcgt cgaattccgc cagctttggc tcgacgccag cttcgacgag gtcggcatgg
     1921 ggaaggtgaa ggcgggcggc agcctgctgt ggatgggcta tcgccgctac ttctgatttc
     1981 cacccccgtg caaacggggc gcgccggacc ggggaacgcc cctctcagcc ggcgccgcgc
     2041 aggcgcacgt tggttctcct gctgatgacg aagtggtgtg gatcgatttt ctgctcggtc
     2101 gcggcgatgc gatactcctc ctcgggccgc cggccgggcg ccttgaccgt acaggatacg
     2161 agcttgccct cggcgttgag ctgcgagagc tcgagcccca tacgttgcac gccgaccccg
     2221 agcttcgccg ctagggcctt gatgcgccag gcggtagggt tggacgtcat gaaggcgagc
     2281 acgctctcgc gcaaggacag gcttgtcacg ggtcgattcc ctacttgtcc catttggcca
     2341 tgtcggccag gtcgacgttg tagccgacgt tctgcgggcc cgcggccgca ctcgccgcct
     2401 tctccacctt gcggaaggtg tcggcgagct ggaggctcaa ctttgcgagc tccgccgggt
     2461 tgggcttgaa cacggccgca ttttcggccc tgaggaaagc ccggccgacc aattcgacat
     2521 agatccgcga cgccaggtcc tggacgccgg cgctcgctgc gggctgcgtg ggggaagcca
     2581 tgaataccgc cttgaagggg aagacgacac atactacgct taactcgagg tgggtaagcg
     2641 aatttccttt gtcagcctct gaaaaaggaa cagccatgga caccgaatcg aaatggaagc
     2701 accgcggtgt gcgcgtggtg cgcgcgaacg agctcgatat caacaccgcg cagacgccgg
     2761 gcatgaaccg cgccgcggcg atcacgaacg cgcgcgccgg agcggagaag ctctgggccg
     2821 ggacggtgat catccatccc aaagcgaaga ccggcgcgca ccatcacggg ccgatcgaga
     2881 gcgtgatcta cgtcgtctca ggccgcgcgc gcatgcgctg gggcgacagg ctcgaattcg
     2941 t
//
LOCUS       DASPZD010000169         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1107396, whole genome shotgun sequence.
ACCESSION   DASPZD010000169 DASPZD010000000
VERSION     DASPZD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107396"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VEM33_03265"
     CDS             <1..123
                     /locus_tag="VEM33_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492166.1"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HYS50874.1"
                     /translation="AADARRMNAFIEERVREMPEQYFWTHKRFKTRPPGEARWY"
     gene            155..1018
                     /gene="dapF"
                     /locus_tag="VEM33_03270"
     CDS             155..1018
                     /gene="dapF"
                     /locus_tag="VEM33_03270"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYS50875.1"
                     /translation="MRLKFTKMQGAGNDFVVIDGATRGIEITPERARRIADRHLGVGC
                     DQVLLVEKSQRGDADFRYRIWNADGGEVEQCGNGARCFVRFVHDKGLTKKNEIRVETL
                     SGIIVPRLEADGQVTVNMGKPLFDPERIPFNASGLRAVRGGKRWPLELSGHEAAVAVL
                     SMGNPHAVQTVADVDSAPVTTEGPLIERHPRFPRRANAGYMQVLARTRIRLRVWERGA
                     GETLACGTGACAAVVTGIREGLLDESVEVDTRGGRLTIRWAGGENDPNSTVWMTGPAV
                     TVFEGEIEIES"
     gene            1008..1667
                     /locus_tag="VEM33_03275"
     CDS             1008..1667
                     /locus_tag="VEM33_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HYS50876.1"
                     /translation="MRADDVARYLRENPGFFESYADMLAEITVPHPHGGRAIPLSDRQ
                     MLGLREKSRALESRLTELLQIGEENDAIGEKMHRLSLALLSAPDLQSLLTVLYLHLRE
                     DFSVPHSALRIWGAEGEAFRGAADAAEFRPVGEQLKRYAGSLSQPFCGPSGDAEAAAW
                     FGEGASHVRSVAHMALRDKECFGMLALGSEDVLRFYPEMGTLYLKRMGELTGAALLRF
                     L"
     gene            1683..>2397
                     /locus_tag="VEM33_03280"
     CDS             1683..>2397
                     /locus_tag="VEM33_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYS50877.1"
                     /translation="MANDSRRRLLEEFLDQLAHQRRLSPGTARNYRRALEQLFALNDG
                     VSLKKLEPQHLRRTVSQLHARGMSGRTIAYLLSGWRGFFNWLVKHRGFPHNPCVGLRA
                     PKSPKALPKALSPDLVARLLETTVEGPAQSRDKAMFELFYSSGLRLAELVSLDAADGE
                     SMQRQAEVTVTGKGAKTRTVPVGSKACAAIAAWLSERARLARPGETALFVGARGRRIA
                     PSSVRYALGHWARRLGLPQH"
BASE COUNT          406 a          779 c          832 g          380 t
ORIGIN      
        1 gcggccgacg cgcgccgcat gaacgccttc atcgaggagc gcgtgcgcga aatgcccgag
       61 caatacttct ggacgcacaa gcgcttcaag acccggccgc cgggtgaggc cagatggtac
      121 tgattctccg cgaattcacc cgcgggcgcg cccgatgagg ctgaaattca cgaaaatgca
      181 gggcgcgggg aacgatttcg tcgtgatcga cggcgccacc cgcggcatcg agatcacgcc
      241 ggagcgggcg aggcgtatcg cagaccgcca tctcggtgtc ggctgcgacc aggtcctgct
      301 ggtggagaaa tcgcagcggg gggacgccga tttccgctac cgcatctgga acgcagacgg
      361 cggagaagtc gagcagtgcg gcaacggcgc gcgctgcttc gtgcgcttcg tgcacgacaa
      421 gggcctcacg aaaaagaacg agatccgcgt cgagacgctc tccgggatca tcgttccgcg
      481 cctcgaggcc gacgggcagg tgaccgtgaa catgggaaag ccgctgttcg atcccgagcg
      541 cattcccttc aacgcgagcg gactccgggc ggtgcgagga ggaaagcgct ggccgctcga
      601 gctctccggt cacgaggctg cagtcgccgt gctctccatg ggcaatccgc acgccgtgca
      661 gaccgtcgcc gacgtggatt cggcgccggt cacgaccgaa ggaccgctga tcgagcgtca
      721 tccgcgattc ccgcggcgcg cgaacgccgg ctacatgcag gtgctcgcgc gcacccgcat
      781 ccggctgcgc gtatgggagc gcggagcggg agagacgctc gcgtgcggca ccggcgcctg
      841 cgctgcggtc gtcaccggca tccgcgaggg gctgctcgac gaatcggtcg aggtcgacac
      901 gcgcggcggc agattgacga tacgctgggc gggcggggaa aatgatccaa acagcacggt
      961 gtggatgacc ggtcccgccg tgaccgtgtt cgaaggggag atcgaaattg agagctgacg
     1021 atgtcgcgcg ctacctgagg gagaatcccg gcttcttcga aagctacgcc gacatgctcg
     1081 ccgagatcac cgttccgcat ccgcacggcg ggcgggccat tccgctctcg gaccgccaga
     1141 tgctcggcct gcgcgagaag agcagggcgc tcgaatcgcg cctgaccgag ctcctgcaga
     1201 tcggcgagga gaacgacgcg atcggcgaga agatgcaccg cctgagcctt gcgctgctct
     1261 ccgcgcccga cctgcaaagc ctcctgaccg tgctctattt gcacttgcgc gaggacttct
     1321 ccgtgcctca ctccgctctg cggatctggg gcgcggaagg cgaggccttc cggggcgcgg
     1381 cggatgccgc ggagttccgt ccggtgggcg aacaactgaa gcgttacgcg gggtccctat
     1441 cgcagccctt ctgtggcccg agcggagacg ccgaggccgc agcctggttc ggcgagggag
     1501 cctcgcacgt tcgctccgtg gcgcacatgg ccctgcgcga caaggagtgc ttcgggatgc
     1561 ttgcgctcgg cagcgaggac gttctgcgtt tctatcctga gatgggcacg ctgtatctga
     1621 agcgcatggg cgagctcacg ggcgcggccc tgctgagatt tctctagccg cggccggcgg
     1681 gaatggcaaa cgactcccgc cgcaggctgc tcgaagagtt tctcgatcag cttgcgcacc
     1741 agcggcggct gagcccggga actgcgcgca attaccgccg cgcgctcgag cagctcttcg
     1801 ccttgaatga cggcgtgtcc ctgaagaaac tcgaacctca gcatctgcgc cgcaccgtgt
     1861 cgcaattgca cgcgcggggt atgtcgggcc ggacgattgc gtacctgctc tcgggctggc
     1921 gaggtttttt caattggctc gtcaagcacc gtggctttcc ccacaacccc tgcgtcggct
     1981 tgcgcgcgcc caaaagcccg aaggcgctgc ccaaggcgct ttccccggac cttgtggcgc
     2041 ggcttctgga aacgacggtg gagggaccgg cgcagtcgcg ggacaaggcc atgttcgagt
     2101 tgttctactc ttccggattg cggcttgccg agctggtgag cctcgatgcc gccgacggcg
     2161 aaagcatgca gcggcaagcg gaggtcacgg tgacgggcaa gggtgccaag acgcgcaccg
     2221 tgccagtcgg aagcaaagct tgcgccgcaa tcgcggcctg gctcagcgag cgcgcgaggc
     2281 tcgccaggcc cggggaaacg gcccttttcg tcggcgcgcg cggccggcgc atagcgccca
     2341 gctcggtgcg ctacgctctc gggcactggg cgcggcgtct cggcctgccg cagcacg
//
LOCUS       DASPZD010000170         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1233589, whole genome shotgun sequence.
ACCESSION   DASPZD010000170 DASPZD010000000
VERSION     DASPZD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233589"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VEM33_03285"
     CDS             <1..818
                     /locus_tag="VEM33_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYS50878.1"
                     /translation="REDKNKEIKAVCVLHNETSTGALTPIAEIRKAIDAAGHPALFLV
                     DTISSLASTEYRHDEWGVDVSIGGAQKGLMLPPGMSFNAIGPKALAASENARLPKSFF
                     AWDDMLAANKSGFFPYTPATNMLHGLSEALDMLHEEGLDNVFARHDRHAEATRRAVRA
                     WGLEILCRDPKYYSPTITGVLLPEGHDADVFRELALDRFDISFGTSFGKFTGKYFRIG
                     HLGDTNDLTILGALASVEMTLALAGVPHRKGGVQAAMDYLISAARAPSRAAAE"
     gene            937..1320
                     /locus_tag="VEM33_03290"
     CDS             937..1320
                     /locus_tag="VEM33_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYS50879.1"
                     /translation="MDKQTFEKGLEIRKTVLGKEYVENALKSADEFSRPFQEFMTEYC
                     WGAVWGREELPKKTRSLLNLAMLGALNKEHELKLHIGGALRNGVSKEEIREVFIQLAI
                     YAGVPAAVVAFRCAREVFAEQKPGR"
     gene            complement(1512..>2335)
                     /locus_tag="VEM33_03295"
     CDS             complement(1512..>2335)
                     /locus_tag="VEM33_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HYS50880.1"
                     /translation="NQANKLLEWVIERAEERVRRRQEKEISRKTAVKKLRLPGKLADC
                     TDTAAEGSELFIVEGDSAGGSAKQARARASQAVLPLRGKILNVASAGKDKLAQNQQLA
                     DLVQALGCGTGVHYRDEDLRYGKIIIMTDADVDGAHIASLLITFFYRQMPKLIDNGHL
                     YLAVPPLYRLTHGAKTVYAKDDADKAALIEREFHGNAKVEIGRFKGLGEMMADQLRVT
                     TMDPARRTLLRVVLLTEDREDTADSVERLMGTKAEARFAFIQERAEFAGEELLDV"
BASE COUNT          406 a          764 c          763 g          402 t
ORIGIN      
        1 tccgcgagga caagaacaag gagatcaagg cggtctgcgt tctccacaac gagacctcga
       61 ccggcgcgct gacgccgatt gcggagatcc gcaaggcgat cgacgccgcc ggccatccgg
      121 cgctgttcct ggtcgacacc atctcgtcgc tcgcctcgac cgaataccgc cacgacgaat
      181 ggggcgtcga cgtgtcgatc ggcggcgcgc agaaggggct gatgctgccg cccggcatgt
      241 cgttcaacgc gatcggcccc aaggcgctcg cagcatccga gaacgccagg ctgccgaaat
      301 cgttcttcgc ctgggacgac atgctcgccg ccaacaagag cggcttcttc ccctatacgc
      361 cggcgaccaa catgctccac gggctctccg aagcgctcga catgctgcac gaggaaggcc
      421 tcgacaacgt gttcgcgcgc cacgaccgcc atgccgaggc gacccgacgg gcggtgcgcg
      481 cctggggcct cgagatcctc tgccgcgatc cgaaatatta ctcgccgacc atcaccggcg
      541 tgctgctgcc ggagggccac gacgccgatg tttttcgcga gcttgcgctc gatcgcttcg
      601 acatctcgtt cgggacgagc ttcggcaagt tcaccgggaa atatttccgc atcgggcatc
      661 tcggcgacac caacgatctc accatcctgg gcgcgttggc gagcgtggag atgacgctcg
      721 cgctcgccgg tgtgccgcat cgcaagggcg gcgtgcaggc cgcgatggat tatctcatct
      781 ccgccgcgcg agcgccctcg cgcgccgccg cggagtagat tttcagctcg tcagatcgat
      841 gatcatgtag cccggatgga gcgaagcgaa atccggggcc gccgttcccg cattccgctg
      901 cgctccatgc gggctacgaa cacacgaggg aagatcatgg acaagcagac attcgaaaaa
      961 gggctggaga tccgcaagac ggttctgggc aaggaatacg tggaaaatgc cctgaagagc
     1021 gccgacgagt tcagccggcc gttccaggaa ttcatgacgg aatattgctg gggcgcggtg
     1081 tggggtcgcg aggagctgcc gaagaaaacc cgcagtctgc tcaaccttgc catgctcggc
     1141 gctctcaaca aagagcacga gctcaagctg cacatcggcg gcgcgctgcg caatggggtg
     1201 agcaaggagg agatccgcga ggtgttcatc cagctcgcga tctacgccgg cgttcccgcc
     1261 gcggtcgtgg cgttccgctg cgcgcgcgag gtgttcgcgg aacagaagcc tggacggtaa
     1321 gcgaacggag cccgacacgc gcggccgggc aacactcttc cctccccctg caagggggag
     1381 ggatagggag ggggtccgaa tgcagaggcg cagaccccct cccgactcgc tgcgcgagcc
     1441 gacctccccc ttgcaggggg aggcgggaga atgccgcgct gtcagtgcga actgatcaac
     1501 cggatctcgt atcacacgtc cagcagttcc tcgccggcga attccgcgcg ctcctggatg
     1561 aaggcgaagc gggcttccgc cttggtgccc atcaggcgct cgaccgaatc cgcggtgtcc
     1621 tcgcggtcct cggtgagcag caccacgcgc agcagcgtgc gcctggccgg atccatcgtg
     1681 gtcactctta gctggtccgc catcatctcg cccagcccct tgaagcggcc gatctcgacc
     1741 ttggcgttgc cgtggaactc gcgctcgatg agcgccgcct tgtcggcgtc gtccttcgcg
     1801 taaacggtct tggcgccgtg ggtgaggcgg tagagcggcg gcaccgcgag atagagatgg
     1861 ccgttgtcga tcagcttcgg catctgccgg tagaagaacg tgatcagcag cgaggcgata
     1921 tgcgcgccgt cgacgtcggc gtcggtcatg atgatgattt ttccgtagcg caggtcctcg
     1981 tcgcggtaat gcacgccggt gccgcagccg agcgcctgga ccaggtcggc aagctgctgg
     2041 ttctgcgcga gcttgtcctt accggccgag gcgacgttga ggattttccc ccgcagcggc
     2101 agcaccgcct ggctggcgcg cgcgcgcgcc tgcttggcgc tgccgccggc cgagtcaccc
     2161 tcgacgatga acaactccga gccttcggca gccgtatcgg tgcaatcggc gagcttgccg
     2221 ggcaggcgca gtttcttcac cgcggtcttg cgggaaatct ccttttcctg gcggcggcgc
     2281 acgcgctcct cggcgcgctc gatcacccat tcgagcaatt tgttggcctg gttgg
//
LOCUS       DASPZD010000171         5962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_346932, whole genome shotgun sequence.
ACCESSION   DASPZD010000171 DASPZD010000000
VERSION     DASPZD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346932"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VEM33_03300"
     CDS             <1..1413
                     /locus_tag="VEM33_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYS50881.1"
                     /translation="ALAEIKELFDPKGLMNPGKIVHPSKQDDRTLFRFKPDYAVPKID
                     AVLDWSEWSVPGAASTGFAAAVEMCNNNGHCRKFDAGAMCPSFRATGDERHVTRGRAN
                     SLRLALSGQLGPGAFTSDEMYETLDLCVSCKGCRRECPTGVDMAKMKIEFLHHYHARH
                     GYSLKDRLIAYLPRYGPLASGFPRTANAVQRLGKRALGFAAQRSLPKWRRSFLSSLRR
                     SSSEGKAGEVVLFVDTFNNYFEPENARAALAVLDRAGYGVHVARATDGSRPLCCGRTF
                     LAAGMVEQAKAEAQRVLAALEPYVGRGVDVVGLEPSCLLTLRDEFKAMLPGGETDALA
                     ESALLFEEFLAREQQTGRLSLELKPLAEKRALLHGHCHQKAFGAMPAVQRALSLVPEL
                     KVETIESSCCGMAGNFGYEARHYAVSMKMAELALLPKVRSAAEDTLLVADGTSCRHQI
                     ADGAAREAVHVAQVLARALA"
     gene            1414..2157
                     /locus_tag="VEM33_03305"
     CDS             1414..2157
                     /locus_tag="VEM33_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYS50882.1"
                     /translation="MILEGAEAALALAPIAVLAGFIQGLTGFGSALVSIPLATFFVPL
                     PFALAVFAVVDFANGLRLGLENPKNAILGDIARMVPLMVAGIVTGTTLLVNLPRQASL
                     TALGVFVLGYALYSLARRGPAATVSRNWAYFAGFCGGLSGTLFGAGGPPYAIYLSHRA
                     LSKEQFRATMTLTTIFSIGLRLVAFAVTGLLMQDGVWIAAAAAIPAGLLAIYFASKIF
                     RVVSREMVMRWVAVLLLASGASLIARGLG"
     gene            complement(2167..2385)
                     /locus_tag="VEM33_03310"
     CDS             complement(2167..2385)
                     /locus_tag="VEM33_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HYS50883.1"
                     /translation="MVETKDEQMQAQMRQRLHELQLEHRDLDTAIHRIADNPSHDQLA
                     LTRMKRRKLLLKDQISWIERQLDPDIPA"
     gene            complement(2401..3189)
                     /gene="lgt"
                     /locus_tag="VEM33_03315"
     CDS             complement(2401..3189)
                     /gene="lgt"
                     /locus_tag="VEM33_03315"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYS50884.1"
                     /translation="MLVHPNFDPVAISIGPIAIRWYGITYLVAFAVSYWLGRLRIARA
                     MAGRVTVAVLDDLLFFCVLGVVFGGRLGYVLFYKAAYYFSHPLEVFAVWHGGMSFHGG
                     FLGVLLAIGWVARKHGLRWIEVTDFFAPLAPLAFAAVRLGNFINGELYGRVTDMPWGM
                     VFRGGGPLPRHPSQLYQFALEGVLLFVILWVYSSKPRPTAALSGAFLLGYGTLRFGVE
                     YFREPDDFLGLLALNLSMGQWLSAPMIAFGIGMLVWAWRRPQKV"
     gene            3263..4939
                     /gene="ilvD"
                     /locus_tag="VEM33_03320"
     CDS             3263..4939
                     /gene="ilvD"
                     /locus_tag="VEM33_03320"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYS50885.1"
                     /translation="MADNWRSRTITQGAARSPNRAMLRAVGFGDGDFQKAIVGVANGH
                     STMNPCNAGIQPLVDRAAAAVKAAGAMPQVFGFPTVTDGIGMGTDGMKYSLVSREVIA
                     DAIETSVNGQMMDGVVCVGGCDKNMPGAMMGMARINVPGIFVYAGTIKPGFWKGQKLT
                     IVSPFEAVGALAAGKISQEDFDGIEKNACPSVGACGGQYTANTMSSSFEALGMSLLGS
                     SQMASPDPEKADSAGESAKVLIDAIRKNIRPRDIITRKSIENAVAMVMATGGSTNAVL
                     HYLAIASAAGVKWSIDDFERIRRRVPVLCNLKPSGPYVATDFHAAGGVPQVLKMLLGH
                     GLVHGECMTITGRTLAEELANVPEEPRAGQDVIRPWSNPVYAQGHLAILKGNLATEGC
                     VAKITGLKQTAITGPARVFDSEPAAMTAILARKIKAGDVIVIRYEGPKGGPGMQEMLA
                     PTSALIGQGLGESVGLVTDGRFSGGTWGMVVGHVAPEAYVGGTIALVKQGDAITIDAK
                     KRLIRLNVSAKELAARRKKWKQPKPRYTRGLLAKYMKLVSTASKGAITDG"
     gene            5034..>5962
                     /locus_tag="VEM33_03325"
     CDS             5034..>5962
                     /locus_tag="VEM33_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HYS50886.1"
                     /translation="MPNRLAQETSPYLQQHAGNPVDWHAWGEEALKRAREEDKPILLS
                     VGYSACHWCHVMAHESFEDPAVAEVMNRLFVNVKVDREERPDLDQIYQLAHQMLAQRP
                     GGWPLTMFLTPDGAPFFGGTYFPKEPRYNLPGFPQLLERVERIYREHRDEIGRQNETI
                     LATFESMQPGVPAHHSELSAEPIAAALRSLKTSFDSRYGGFGGAPKFPHPADFEFCLR
                     RSSAAGDGAAERVATFTLERMALGGIYDQIGGGFSRYSVDARWQIPHFEKMLYDNGPL
                     LRLYCDAWTVTKNPLFARVADETAGWVMREMQSP"
BASE COUNT         1047 a         1941 c         1996 g          978 t
ORIGIN      
        1 gcgctcgccg agatcaagga gcttttcgat ccgaaggggc tgatgaatcc gggcaagatc
       61 gtgcacccgt ccaagcagga cgacaggacg ctgttccgct tcaagcccga ctacgccgtc
      121 ccgaagatcg acgcggtgct cgactggagc gaatggagcg tgccgggcgc cgcgagcacg
      181 gggttcgcgg cggcggtcga gatgtgcaac aacaacgggc actgccgcaa gttcgacgcc
      241 ggggcgatgt gcccgtcttt ccgcgccacc ggcgatgaga ggcacgtcac gcgcgggcgc
      301 gccaactcgc tgcgccttgc gctttccggc cagctcggtc ccggcgcctt cacttcagac
      361 gagatgtacg aaacgctgga cctgtgcgtt tcctgcaagg gctgcaggcg cgaatgcccg
      421 accggcgtcg acatggcgaa gatgaagatc gagttcctgc atcactatca tgcccgccac
      481 ggctattcgc tgaaggacag gctgatcgcc tacctgcccc gctacggtcc tctggcctcg
      541 ggcttcccac ggacggcgaa cgcggtccag aggctgggca agcgcgccct cggcttcgct
      601 gcccagcgct ccctgccgaa gtggcgccgc tcgttcctca gctcgctgcg ccgatcgagc
      661 agcgaaggga aggccggcga ggtcgtgctc ttcgtcgaca cgttcaacaa ctacttcgag
      721 ccggaaaacg cacgcgccgc tctcgccgtg ctcgaccggg ccggctacgg cgtgcatgta
      781 gcgcgtgcta cagatggttc gcggccgctg tgctgcggcc gcactttcct cgcggcggga
      841 atggtcgagc aggcgaaggc cgaggcgcaa cgggtgctgg ccgcgctcga gccctacgtg
      901 gggcgcgggg tcgatgtcgt cggcctcgag ccttcctgcc ttctgaccct gcgcgatgaa
      961 ttcaaggcga tgctcccggg tggcgagacc gacgcgctcg ccgaaagcgc gctgctcttc
     1021 gaggagttcc tcgcacgcga gcagcagacc ggccgtctct cgctcgagct caagccgctc
     1081 gccgagaagc gcgcgctcct gcacggccat tgccaccaga aggccttcgg cgccatgccc
     1141 gcggtccagc gggcgctgtc gctcgttccg gagctgaaag tcgagacgat cgaatcgtcg
     1201 tgctgcggaa tggcgggaaa tttcggctac gaggcccggc actacgcggt ctcgatgaag
     1261 atggccgagc tcgcgctgct tcccaaggtc cgctcggcgg ccgaggatac gctgctcgtc
     1321 gccgacggta ccagctgccg ccatcagatc gccgacggag cggcgcgcga ggccgttcac
     1381 gtcgcgcaag tgctggcgcg cgcgctggcc tagatgattc tcgaaggcgc cgaagcggcg
     1441 ctcgcgctcg cgccgatcgc cgtcctagcc ggtttcatcc agggcctcac gggcttcggc
     1501 tcggccctcg tctcgattcc gctcgcgacc ttcttcgtcc cgctgccttt tgcgctcgcg
     1561 gtattcgcgg tcgtcgattt cgccaacggc ctgaggctgg gactggagaa cccgaagaac
     1621 gcgatcctcg gcgacatcgc gcgcatggtg ccgctgatgg tcgccgggat cgtcaccggg
     1681 accacgctgc tcgtaaatct tccgcgccag gcgtcgctca ccgcgctcgg cgtcttcgtc
     1741 ctgggctacg cgctctacag cctcgcgcgc cggggccccg cggcgacggt gagccggaac
     1801 tgggcgtact tcgccggctt ctgcggaggg ctgtcgggga cgttgttcgg cgcgggcggg
     1861 ccgccgtacg cgatctacct ctcgcaccgc gcgctcagca aggagcagtt ccgcgcgacc
     1921 atgaccctca ccacgatttt cagcatcggg ctgcgcttgg tggcgttcgc ggtcacgggc
     1981 ctcttgatgc aggacggcgt gtggatcgca gcggcggcag cgattcccgc gggcctgctc
     2041 gcgatctact tcgcgtcgaa gatattccgc gtggtttcgc gcgagatggt gatgcgctgg
     2101 gtggcggttc tgctgctggc gagcggcgcg tcgcttattg cgcgcgggct cggttgaggc
     2161 ttttcttcag gcggggatgt cgggatcgag ttgtctctcg atccaggaga tctggtcttt
     2221 gaggagaagc ttgcggcgct tcattcgggt gagggcgagc tgatcgtggg aggggttgtc
     2281 ggcgatccgg tgaatcgcgg tgtccaggtc gcggtgctcg agctgcagct cgtgcaaccg
     2341 ctgcctcatc tgcgcctgca tctgttcgtc cttcgtctcg accatggcac cgcttttatt
     2401 ttagaccttt tgcggcctgc gccaagccca aacgagcata ccgatgccga atgcgatcat
     2461 cggcgccgac agccactggc ccatggacag gttgagcgca agaagtccaa gaaaatcatc
     2521 gggctcgcga aaatactcga ccccgaatcg caaggtgccg tagcccagga gaaaggcgcc
     2581 ggacagcgcc gcagtgggcc tgggcttcga agaatagacc cacaggatca cgaagaggag
     2641 caccccctcg agggcgaact ggtagagctg agagggatgg cgcggaagcg gtccacctcc
     2701 gcggaacacc atgccccagg gcatatcggt gaccctgccg tagagctcgc cgttgatgaa
     2761 attgccgagc cgcaccgcgg cgaacgcaag gggggccagc ggcgcgaaga aatccgtgac
     2821 ctcgatccag cgcaggccgt gctttcgcgc gacccaccca atcgcgagca gcacgccgag
     2881 gaaccctccg tggaaggaca tgccgccgtg ccagaccgcg aagacctcca gcggatggga
     2941 gaagtaatac gccgccttgt agaagagcac gtatccgagc ctgccgccga acaccacgcc
     3001 gagcacgcag aagaacagca ggtcatccag caccgcgacg gtgacgcgcc ccgccatcgc
     3061 gcgcgcgatg cgcaagcgcc cgagccaata ggacacggcg aatgcgacga gatacgtgat
     3121 cccgtaccag cggatcgcga tcgggccgat cgatatcgcg accggatcga agttcggatg
     3181 gacgagcatg gagggccgcg gcgcgctttc aagtaaaatg cagccgaagc gtaactgatt
     3241 ttctgcattc ggagtttttc ccatggcgga caactggagg agcaggacga tcactcaggg
     3301 cgcggcgcgc tcgccgaacc gcgcgatgct gcgcgcggtc ggcttcggcg acggggattt
     3361 ccagaaagcg atcgtcggcg tcgccaacgg ccactcgacc atgaatccct gcaacgccgg
     3421 aatccagccg ctggtggatc gcgcggccgc ggcggtgaaa gccgccggcg ccatgcccca
     3481 ggtattcggc tttcctaccg tcaccgacgg catcggcatg ggcaccgatg gcatgaagta
     3541 ttcgctggtc tcgcgcgagg tgatcgcgga tgcgatcgaa acctcggtga acggccagat
     3601 gatggacggc gtggtctgcg tgggcggctg cgacaagaac atgcccgggg cgatgatggg
     3661 gatggcacgg atcaacgtgc cgggaatctt cgtttacgcc ggcacgatca agccgggatt
     3721 ctggaagggg cagaagctca ccatcgtctc gcctttcgag gcggtgggtg cgctcgccgc
     3781 cgggaagatc tcgcaggagg acttcgacgg catcgagaag aacgcctgcc cttcggtcgg
     3841 tgcctgcggc gggcagtaca ccgcgaacac catgtcctcc tcgttcgagg cgctcggcat
     3901 gagcctcctc ggctcctcgc agatggcctc gcccgacccc gagaaggcgg actccgcggg
     3961 ggaatcggcg aaagtcttga tcgacgcgat ccgaaagaac attcgtccgc gcgacatcat
     4021 cactcgcaaa tcgatcgaga acgcggtcgc gatggtcatg gcgaccggcg gttcgaccaa
     4081 cgcggtgctg cactatctcg cgatcgcgtc cgctgccggc gtcaaatgga gcatcgacga
     4141 cttcgagcgc atcaggcgcc gcgtgcccgt gttgtgcaac ctgaagccct cgggaccgta
     4201 cgtcgccacg gattttcacg ccgcgggcgg cgtcccgcag gtactgaaga tgctgctcgg
     4261 gcacggcctg gtgcacggcg agtgcatgac gatcaccggg cgcacgctcg ccgaagagct
     4321 cgcgaacgtg cccgaggagc ccagagccgg ccaggacgtc atccgcccct ggagcaatcc
     4381 cgtgtacgcg caagggcacc tcgcgatcct gaagggcaat ctcgccaccg aaggctgcgt
     4441 cgccaagatc acggggctca aacagaccgc gatcacgggc ccggcgcgcg tgttcgactc
     4501 ggagccggcg gcgatgactg cgatcctcgc gaggaagatc aaggcgggcg acgtgatcgt
     4561 gatccgctac gaaggaccca agggaggccc cggcatgcag gagatgctgg cgcccacttc
     4621 cgcgcttatc ggccagggcc tcggcgaatc ggtgggtctc gtcaccgacg gacgcttctc
     4681 gggcggcacc tggggcatgg tcgtggggca tgtcgcgccg gaggcctacg tgggcggaac
     4741 gatcgcgctg gtgaagcaag gcgacgcgat cacgatcgac gcgaagaagc gccttatccg
     4801 gctgaacgtc tccgcgaaag agctcgccgc gcgcaggaaa aaatggaagc agcccaagcc
     4861 gcgctatacg cgcggcctcc tcgcgaagta catgaagctc gtgtccaccg cgagcaaagg
     4921 cgccatcacg gatggctgag aaaaccgggg acagatcacg gttttcttgc cgagcggaat
     4981 tccaggtggc cgtctgggaa aaacgtggtc tgtccccggt taatatctct gtcatgccca
     5041 atcggctcgc gcaggaaacc agcccctatc tccagcagca cgcggggaat cccgtcgact
     5101 ggcacgcctg gggagaagaa gcgctcaaac gggcgcgcga ggaggacaag ccgatcctcc
     5161 tctcggtcgg atactccgcc tgccactggt gccacgtgat ggcgcacgag tccttcgaag
     5221 acccggcggt ggccgaggtg atgaatcgcc tgttcgtcaa cgtcaaggtg gaccgcgagg
     5281 agcgcccgga tctcgaccag atctatcaac tcgcgcacca gatgctcgcc cagcggccgg
     5341 gcggctggcc gctcaccatg ttcctcacgc ccgacggcgc gcccttcttc ggcggcacgt
     5401 acttccccaa ggagccgcgc tacaaccttc cgggattccc gcagctgctc gagcgggtcg
     5461 agcggatcta ccgcgagcac cgcgacgaga tcgggcgcca gaacgagacg attctcgcta
     5521 ccttcgaaag catgcaaccc ggcgtgccgg cgcatcactc ggagctgtcg gcggagccca
     5581 tagccgcggc tctgcgcagc ctgaagacga gcttcgactc gcgctacggc gggttcggcg
     5641 gtgcccccaa attcccgcac ccggcggatt tcgaattctg cctgcggcgc tcttccgcgg
     5701 cgggcgacgg cgcggccgag cgggtcgcca cgtttacgct cgagcgcatg gcgctcgggg
     5761 gcatctacga ccagatcggg ggcgggttct cgcgctacag cgtggacgcg cggtggcaga
     5821 tcccgcattt cgagaaaatg ctctacgaca acggcccgct gctgcgcttg tattgcgacg
     5881 cgtggaccgt gacgaagaac ccgctgttcg cgcgcgtggc cgatgagacc gcggggtggg
     5941 tgatgcgcga gatgcagtct cc
//
LOCUS       DASPZD010000172         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_349683, whole genome shotgun sequence.
ACCESSION   DASPZD010000172 DASPZD010000000
VERSION     DASPZD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349683"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VEM33_03330"
     CDS             complement(<1..609)
                     /locus_tag="VEM33_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HYS50887.1"
                     /translation="MAEAQKSGTIVQCIGAVIDIEFPREGMPGVYDALVLEDSDAKSF
                     AEKGLTFEVEQQLGDGVVRCIALGSSDGLRRGMKVKSTGGPISVPVGKGTLGRIMDVL
                     GRPIDEAGPIQSEERRSIHRAAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFG
                     GAGVGKTVNMLELINNIATKHSGLSVFAGVGERTREGNDFYHE"
     gene            complement(816..1679)
                     /gene="atpG"
                     /locus_tag="VEM33_03335"
     CDS             complement(816..1679)
                     /gene="atpG"
                     /locus_tag="VEM33_03335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HYS50888.1"
                     /translation="MPGTKEIRMKIRSVQNTRKITKAMEMVAASKMRKAQERMRQARP
                     YGEKIRNVAAHISHANPEYRHPFLVERDTVKRIGVILVTSDRGLCGGLNTNVQRLALG
                     KYKEWQGQGEEVDVCCIGNKGFGFMQRLGANIVSHAIQLGDWPPMDRLIGAVKVMLDA
                     YTTDTFDRLMIFYTRFINTMKQEPVMEQLLPLSGERLGAPEGSWDYLYEPDAKAVLDQ
                     VLTRYIEAIIYQTVTDNKASEHSARMVAMKAASDNAETLIDELTLVYNKNRQAGITKE
                     ISEIVGGAAAV"
     gene            complement(1783..>2602)
                     /locus_tag="VEM33_03340"
     CDS             complement(1783..>2602)
                     /locus_tag="VEM33_03340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HYS50889.1"
                     /translation="TMGEYFRDRGQDALIIYDDLTKQAWAYRQLSLLLRRPPGREAYP
                     GDVFYLHSRLLERGARCSEAWVERFTEGKVKGKTGSLTALPIIETQAGDVTAFVPTNV
                     ISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKIMKRRDTGGGVRLALAQYR
                     ELAAFAQFASDLDEATRKQLERGRMVTELMKQPQYQPLAVWEMALTLFAINNGYFDSI
                     DVKKALAADKSLRDHMKDKYAALVKSMEDKKDLSAEDEKALKAAIEDWKKSGSF"
BASE COUNT          388 a          845 c          829 g          540 t
ORIGIN      
        1 ctcgtggtag aagtcgttgc cctcgcgcgt cctctcgccc acgccggcga acaccgagag
       61 gcccgagtgc ttggtcgcga tgttgttgat gagctcgagc atgttgacgg tcttgcccac
      121 gccggctccg ccgaagaggc cgatctttcc ccccttggcg aaagggcaga tcaggtcgat
      181 caccttgatg ccggtctcca gcagctcgac cgagggcgag agctcgtcga acttgggcgc
      241 cgcccggtgg atcgagcggc gctcctcgct ctggatcggc ccggcctcgt cgatcgggcg
      301 gccgaggaca tccatgatgc ggccgagcgt gcccttgccg accggcaccg agatcgggcc
      361 acctgtgctc ttcaccttca tgccgcggcg caggccgtcg gaggacccga gcgctatgca
      421 gcgcaccacg ccgtccccga gctgctgctc gacctcgaag gtgaggcctt tttcggcgaa
      481 gctcttcgcg tccgagtcct cgagcacgag cgcgtcgtag acgccgggca tcccctcgcg
      541 cgggaattcg atgtcgatca ccgcgccgat gcactggacg atggttccgg atttctgggc
      601 ttctgccatg gtgttttcct agtggcgctt tccaaattcg gtttcccctc ccatcccctc
      661 ctcatcttga ggaggggtgc ccgcgaagcg ggcggggtgg tagaggggtg cctgcgcagc
      721 aggcggggtg gtcttgcgcg ccgaccacca ccccgcgcct tcggcgcacc cctcctcaag
      781 agaggagggg aaaaaaaagg cgcaggtcgc tcgattcata ctgccgccgc tccgccgacg
      841 atttctgaga tctccttggt gatccccgcc tgccggttct tgttgtagac gagcgtgagc
      901 tcgtcgatca gcgtttcggc gttgtccgat gcggccttca tcgcgaccat gcgcgccgaa
      961 tgttccgagg ccttgttgtc ggtcaccgtc tggtagatga tcgcctcgat gtagcgcgtg
     1021 agcacctggt cgagcacggc tttcgcgtcg ggctcgtaga ggtagtccca ggatccctcg
     1081 ggcgcgccca ggcgttcgcc cgaaagcggc aggagctgct ccatcaccgg ctcctgcttc
     1141 atggtgttga tgaagcgcgt gtagaaaatc atcagccggt cgaatgtgtc cgtcgtatac
     1201 gcgtccagca tcactttcac ggcgcctatc agcctgtcca tcggcggcca gtcgccgagc
     1261 tggatggcgt gcgaaacgat gtttgcgccc aggcgctgca tgaaaccgaa acccttgttg
     1321 ccgatgcagc agacgtccac ctcttcgccc tggccctgcc attccttgta ctttccgagc
     1381 gcgagccgct gcacgttggt gttgagcccg ccgcacagtc ccctgtcgct ggtgacgaga
     1441 atcacgccga tgcgcttcac cgtatcgcgt tcgacgagga aagggtgccg gtactccgga
     1501 ttggcgtggc tgatgtgcgc ggcgacgttg cggatcttct cgccgtaggg gcgcgcctgg
     1561 cgcatgcgct cctgcgcctt cctcatcttg gaggcggcga ccatctccat cgccttggtg
     1621 atcttccggg tgttctgcac actccggatt ttcatcctga tttccttggt gcccggcatc
     1681 ggtcaaaacc ggggacagac cacgtttttc atcagaattc tcttgtaagg tagcaggaca
     1741 gcgcgcgaaa aacgtggtct gtccccggtt tatcctttgg catcagaagc taccgctctt
     1801 cttccagtct tcgatggccg ctttcagcgc cttctcgtcc tcggcggaca ggtctttctt
     1861 gtcttccatg ctttttacga gcgcggcgta cttgtccttc atgtggtcgc gcagcgactt
     1921 gtcggcggcg agcgccttct tcacgtcgat cgagtcgaag tagccgttgt tgatggcgaa
     1981 gagcgtgagc gccatctccc acaccgcgag cggctggtac tgcggctgct tcatcagctc
     2041 ggtcaccatg cggccgcgct ccagctgctt tcgcgtcgcc tcgtcgaggt cgctcgcgaa
     2101 ctgcgcgaaa gcggcgagct cgcggtactg cgcgagggcg aggcgcaccc cgccgcccgt
     2161 gtcgcggcgc ttcatgatct tggtctgggc cgcgccgccc acccgcgaca ccgagatgcc
     2221 ggcgttgatc gcgggccgga tgcccgcgtt gaagagatcg gtctcgagga agatctggcc
     2281 gtcggtgatc gagatcacgt tggtcggaac gaacgcggtc acgtcgcccg cctgcgtttc
     2341 gatgatcggg agagcggtaa gcgagccggt cttgcccttc accttccctt cggtgaagcg
     2401 ctcgacccag gcctcggagc agcgcgcgcc gcgctcgagc aggcgggagt gcaggtagaa
     2461 cacgtccccc gggtacgcct cgcgccccgg cgggcggcgc agcaggagcg agagctgccg
     2521 gtacgcccag gcctgcttgg tgagatcgtc gtagatgatg agagcgtcct ggccgcggtc
     2581 gcggaaatac tcgcccatgg tg
//
LOCUS       DASPZD010000173         8000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_22981, whole genome shotgun sequence.
ACCESSION   DASPZD010000173 DASPZD010000000
VERSION     DASPZD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22981"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="VEM33_03345"
     CDS             <1..2058
                     /locus_tag="VEM33_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HYS50890.1"
                     /translation="VDARGDVRLQRNGDTLKGPALRYSIRDTTGVFEKPDFIFKPRPK
                     AGQPPVSARGQAETIEFLGEGQYRIKDGFFTSCKPGDDGWLVKADELELDFTRDVGTA
                     RGARVYFEGLPVIAAPSFDFSLNNQRKSGFLPPSIGVTGKSGPEVGIPYYLNLAPNYD
                     LTLTPRYMEKRGLQLAEQFRYLQKDYKGDFSAEVLPNDRAADGDRRSAMSLTGSYNRD
                     ATVLGGLNLNKVSDDNYFRDLATRINIASQTILPREGFLTYIGKWWDTGSYSATARVQ
                     RFQILQDPDNPIVVYDRTPQLTLSALRQDIGGFDFGSSVEFVDFSHPTLVNGKRSTLY
                     PSLSLPLITPGAFLTPKVGVHYTYYSLYEIPVIEVPPSSGNFIPAFSNSISRTLPIFS
                     VDSGLIYERETNALGQAVIQTLEPRFYYVNIPFRDQNQIPLFDTAIADFNYSQIFSEN
                     MFSGADRINDARQLTAAVTSRMLLPSTGQEVLRGTFGQRYYFRDQRVTLTPGDTPRTY
                     NASNWLAALSGRVSQHWTFDGATEYDQRDYRTERLTLAARYRPEGLKALNLSYRYLSG
                     DITTTAPLKQVDLSAQWPIAGHLYGVARFNYSLVDSRIVEGIGGLEYGADCWTSRIIM
                     TRFALPGGTSSSSVFLQLELNGLSRLGSNPLEALKRNIPGYQRINAPSADRKPGDPFD
                     FYN"
     gene            2077..3390
                     /locus_tag="VEM33_03350"
     CDS             2077..3390
                     /locus_tag="VEM33_03350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYS50891.1"
                     /translation="MRFRLAILLLAACGSALAQSPRAEQAAPARVDLIDRIVAVVNKE
                     VITQYELTERMNRVQKELQRRGTSLPARAEIERQVLERLIIERVQLQFARESGVRVDD
                     LELDRTVSRVAEGNKLSLTEFRQTLERDSIRFDAFREELRSEILMNRLRDREVTGKIT
                     VSEGEIENLLLEQSQRKDTATEYNVAHILIRVPEQATPEQLESRRARAEEVVRRLKDG
                     TEFAQLAATHSDAPDALQGGVMGWRSQQRLPELYVDALGGLKPGDISNVFRSAAGFHV
                     LKLLELRGAGAPLLVDQAHVRHILARTNDLVSEDEAKRKLLNLRERIVNGVNFAELAR
                     LNSDDGSASRGGDLGWIYPGDTVPEFERAFAELKQMDVSQPVKTPFGWHLIQVLERRT
                     ADMSSERKRIEARKTILDRKGDEAYQEWLRQLRDRAYIEMRLEER"
     gene            3459..4427
                     /gene="pdxA"
                     /locus_tag="VEM33_03355"
     CDS             3459..4427
                     /gene="pdxA"
                     /locus_tag="VEM33_03355"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYS50892.1"
                     /translation="MAVTSGEPAGIGPDVCLALARKRFAARIVVLGDREMLESRARQL
                     GLRVQLSDPGAGLTPDSIACLHIPLSHPCRAGALDPRNASYVISLIDRAIEGCKAGEF
                     AALTTAPVHKGVINDGGIPFIGHTEYLAERTGAAQVVMMLAGGGLRVALATTHLALKD
                     VPRALTREGLERTIRVLHAGLIRRFGISAPRILVAGLNPHSGEGGYFGREEIEVIGPV
                     LEKLRAEGLRLTGPLPADTLFVPDRLAAADCALAMYHDQGLPVLKYASFGRGINVTLG
                     LPMIRTSVDHGTAIELAGTGQAEPGSLIEAIEAAIEMAQAGMRTAT"
     gene            4431..5216
                     /gene="rsmA"
                     /locus_tag="VEM33_03360"
     CDS             4431..5216
                     /gene="rsmA"
                     /locus_tag="VEM33_03360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYS50893.1"
                     /translation="MATGRHVPRKRFGQHFLVDRGIIERIVSAIDPRPGDHIVEIGPG
                     LGALTAPLLERVEHLHVVELDRDLSGRLRERYPVDRLTVHQADALRFDFAALPAPLRV
                     VGNLPYNISTPLLFHVAAAAARCTDLHFMLQHEVVERMIANPSEPEYGRLSVSLQYRF
                     RLERLFTVAPGSFRPAPKVHSAVVRLVPRPMAELDALNEAGFSGIVARAFSMRRKTLR
                     NNLSGLISPSDLAELGIDPGKRAQNLPVAAFVTIANRLAQRRT"
     gene            5288..5959
                     /locus_tag="VEM33_03365"
     CDS             5288..5959
                     /locus_tag="VEM33_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50894.1"
                     /translation="MGLLDDLKKQADLVKTQQILQQNLQGDKLKLVEDKMKQTFQYVH
                     ELLKQLAVLKPTSPLVYSIPGVADFRNLLFTESFIDYRKKRINDKEYFDTMHFFVKWA
                     GPDTLVIEKDMPPAMQRIRESLWLSKVKFVEEEKKSARGFVVGAKFVIPTAVLTDIVI
                     KADHEQGKLHFQATHLFGLGVEYFSIPAQEVSDDMLEDFAKALIGQQSAIRKYRVAGA
                     PGTFR"
     gene            complement(6003..6386)
                     /gene="gloA"
                     /locus_tag="VEM33_03370"
     CDS             complement(6003..6386)
                     /gene="gloA"
                     /locus_tag="VEM33_03370"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HYS50895.1"
                     /translation="MRILHTMLRVGDLERSLRFYTEVLGMRLLRRKDYPEGKFTLAFV
                     GYGDETDTAVIELTYNWGVDKYELGSAFGHIAVAVDDAYKACEEVKLRGGKVTRDAGP
                     MKHGTTVIAFIEDPDSYKIELIERK"
     gene            complement(6400..7113)
                     /locus_tag="VEM33_03375"
     CDS             complement(6400..7113)
                     /locus_tag="VEM33_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYS50896.1"
                     /translation="MTFLRSLVFLIAEILVTPPYAILALATFPLPRLARYRVISGWSR
                     AMIWLAKNVLGIHYRVVGLENLPRTPGVILAKHQSAWETLAFQVIFPPQVLVLKRELL
                     WIPFFGWGLALTSPIAIDRSRGVRALNRMAELGGERLAQGFWIAIFPEGTRVKPGERR
                     KYHEGGAWLAVKCGASVVPVAHNAGLFWGRNAFLKRPGTVTVEVGPPIDSRAHTPESL
                     NLSVEEWIEGRMARLCPQR"
     gene            complement(7110..7643)
                     /gene="gmhB"
                     /locus_tag="VEM33_03380"
     CDS             complement(7110..7643)
                     /gene="gmhB"
                     /locus_tag="VEM33_03380"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HYS50897.1"
                     /translation="MKLIILDRDGVINYDSDEFIKHPDEWRPIPGSLDAIARLTQGGW
                     RVVVATNQSGIGRGLMDMASLNAINSKMHRVVNQAGGRIEAVFYCPDTAESQSPCRKP
                     NPGMFFAVAERLNVALERAPCVGDSLRDLQAAAAVGAHPILVLSGKGAKTRSAGQLPE
                     GTVVYPDLAAVAHALCQ"
     gene            complement(7640..>8000)
                     /locus_tag="VEM33_03385"
     CDS             complement(7640..>8000)
                     /locus_tag="VEM33_03385"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HYS50898.1"
                     /translation="PEAASLAAANKRIANILRQAESKGESFADTDASALKEPAEILLF
                     EALQTASRSAKPLFDSGDFAGYLKAFASLKSPVDSFFDAVMVMTDDKKLRENRLALLT
                     AMRKAMNRFSDISKLAA"
BASE COUNT         1511 a         2535 c         2474 g         1480 t
ORIGIN      
        1 gtcgacgccc gcggcgatgt gcgcctccag cgcaacggcg atactttgaa gggccccgcg
       61 ctgagatatt cgatcaggga tacgaccgga gtattcgaga agccggactt tattttcaag
      121 ccgcgcccga aggccggcca gccacccgtc tcggctcgcg ggcaggccga gacgatcgag
      181 ttcctgggtg aaggtcagta cagaatcaag gacggcttct tcacctcgtg caagccgggt
      241 gacgatggct ggctggtcaa ggccgacgag cttgagctgg atttcacgcg cgacgtggga
      301 accgcccgcg gcgccagggt gtatttcgag gggctgcccg tcatcgccgc gccttctttc
      361 gacttctctc tgaataacca gcgcaaatcg ggctttctcc cgccttcgat cggggttacc
      421 gggaagagcg gacccgaggt cggaattccg tattacctga atctcgcgcc aaactacgac
      481 ctcacgctca cgccgcgtta catggaaaag cgcggattgc agctcgcgga gcagttccgt
      541 tatttgcaga aggactacaa gggggatttc agcgcggagg tgctcccgaa tgatcgtgca
      601 gcggatggcg atcgccgctc ggcgatgagc ctgacaggca gctacaaccg cgacgcaacc
      661 gtcctgggcg gacttaacct gaacaaagtt tccgacgaca actatttccg cgaccttgcg
      721 acccgcatca acatcgcttc gcagacgatc ctgcctcgtg agggcttctt gacctacatc
      781 ggcaagtggt gggatacggg aagctactct gctacggcac gggtccaacg cttccagatc
      841 cttcaggatc cggacaatcc gatcgtggtc tacgaccgca ccccgcagct caccttgtct
      901 gctctgcgcc aggacatcgg tggcttcgat ttcgggtcct cggtcgagtt cgtggacttc
      961 agccatccta cgctggtgaa cggcaagcgc tcgacgctct atccgagcct cagcctgccg
     1021 ctcatcactc cgggtgcttt cctcacgccg aaggtcggtg tgcactacac gtactattcg
     1081 ctttacgaga tcccggtgat cgaggtcccc ccgagttcgg gcaacttcat cccggcattc
     1141 tcgaattcga tcagccgcac gcttcccatt ttcagcgtcg acagcgggct gatctacgaa
     1201 agggagacca acgcgctcgg gcaagcggtc atacagacgc tcgagccgcg cttttactat
     1261 gtgaacatcc cctttcgcga ccagaatcaa attcccctgt tcgacactgc aatcgcggac
     1321 ttcaactatt cccagatctt ttccgaaaac atgttttccg gcgccgaccg catcaacgac
     1381 gcgcgccagc tcacggcagc ggtcacgtcg cgaatgcttc tgccttcgac cgggcaggag
     1441 gtgctgcgcg gcacgttcgg tcagcgctac tatttcaggg accagcgggt cacgctcact
     1501 cccggcgaca cgccgcgcac ttacaacgca tcgaactggc tcgccgcgct gtccgggcgc
     1561 gtgagccagc actggacttt cgacggtgct accgaatacg atcaacgcga ctaccgcacc
     1621 gagcgcctga ccctggcggc gcgctatcgg cccgaggggc tcaaagcgct caacctgagc
     1681 taccgctacc tgagcgggga catcacgacg acggctccgc tcaagcaggt ggacttgtcg
     1741 gcgcagtggc cgatcgccgg gcacttgtac ggcgtcgcgc gcttcaacta ttcgctggtc
     1801 gatagccgga tcgtcgaagg cataggggga ctcgagtacg gagccgactg ctggacaagc
     1861 cgcatcatca tgacgagatt cgcgctgcct ggcggcacct cgtcgtcaag cgttttcctg
     1921 cagctggagc tgaacggcct ttcgcgcctc gggtccaatc ccctcgaggc cttgaaacgc
     1981 aacattccgg gctatcagcg gatcaacgca ccttcagccg acaggaagcc cggcgaccca
     2041 tttgatttct acaactgatg gcttgacaat gaaacgatga gatttcggct tgccattctg
     2101 ctgttggcgg cgtgcggttc agcgctcgcg caaagccccc gtgcggagca ggccgcaccg
     2161 gcgcgcgtcg acctgatcga ccggatcgta gcggtagtca acaaggaagt gatcacgcag
     2221 tacgagctga cggagcggat gaatcgcgtg cagaaggagc tgcagcgccg cgggacatcc
     2281 cttccagctc gcgccgagat cgagcgccag gtgttggagc gcctcatcat cgaaagagtt
     2341 cagctgcaat ttgcccggga gtcgggggtg cgcgtggacg atttggagct cgatcgcacc
     2401 gtcagccggg tcgcggaggg gaacaagctc tcactgacgg aattcaggca gacgctcgag
     2461 cgcgattcga tccggttcga cgcatttcgg gaagagctgc gcagcgagat tctgatgaat
     2521 cgcttgcggg accgcgaagt caccggcaag atcacggtca gcgagggtga gatcgagaat
     2581 ctgctcctcg agcagagcca aagaaaagac accgcgaccg aatacaacgt cgcgcacatc
     2641 ctgatccgcg tgcccgagca ggcgacccct gaacagctgg aaagccggcg tgcccgcgcc
     2701 gaggaagtgg tcaggcggct caaggacggc acggagttcg cccagctcgc cgcaacccac
     2761 tccgatgcac ccgatgcgct tcagggaggt gtaatgggtt ggcgcagtca gcagcggctg
     2821 ccggaattgt acgtcgatgc tctggggggc ctgaagcccg gagacatcag caacgtgttt
     2881 cgaagcgccg ccggattcca cgtgctcaag ctcctcgagc tgcgcggcgc cggtgcgcct
     2941 ctcctcgtgg accaggcgca cgtgcgccat atcctggcgc gtaccaacga tctcgtttcc
     3001 gaggacgagg ccaagaggaa gctgctcaac ctgcgggaac gcatcgtaaa cggagtgaac
     3061 ttcgcggagc tcgcccggct caattcggac gacggctcgg cttcccgggg aggagatctc
     3121 ggatggatct atccgggcga taccgtaccg gaattcgagc gggcttttgc tgagctgaag
     3181 cagatggatg taagccagcc cgtcaagacc cccttcggat ggcatctcat ccaggtgctc
     3241 gagcggcgca ccgcggacat gtcctcggag cgcaagcgca tcgaggcgcg caagaccatt
     3301 ctcgatagaa agggtgacga ggcctatcag gaatggcttc gccagctgcg cgaccgcgcc
     3361 tacatcgaga tgcggctcga agaacggtga acgcccgact cgaaaaaccg gcggcgtctc
     3421 gggcaggcgt tgcgtcgcgc tcgaattccg ttccggtcat agccgtgacc tcgggggagc
     3481 cggcgggcat cggccccgat gtctgtctgg ccctcgcgcg gaagaggttc gccgcgcgca
     3541 tcgtggtact cggagaccgg gagatgctcg agagccgggc ccggcagctc gggctccgcg
     3601 ttcagctgag cgatcccggc gcaggcttga cgccggattc gatcgcatgc ctgcatatcc
     3661 cgctttcgca cccctgccgg gcgggcgcgc tcgatccgcg caacgcgtcc tacgtgattt
     3721 cactcatcga ccgtgcaatc gaaggctgca aggccggaga gttcgccgcg ctcacgaccg
     3781 ctcccgtgca caagggcgtc atcaacgacg gcggcatccc tttcatcggc cacacggagt
     3841 atctcgccga gcgcaccggc gccgctcagg ttgtcatgat gctcgcgggc gggggcctgc
     3901 gcgtcgcgct cgcgacgact cacctcgcct tgaaggatgt gccgcgcgcg ctgacgcgcg
     3961 aaggtctcga acgcaccatc cgggtcctcc acgcggggct tatccgccgg ttcggaatct
     4021 cagcgccgcg gatcctggtt gccggtctca atccgcacag cggcgagggc ggctacttcg
     4081 ggcgagagga aatcgaagtg atcgggcccg ttctcgagaa attgcgcgcg gaaggtctgc
     4141 ggctcaccgg gccgctgccg gcggatacgc tgttcgttcc ggatcggctc gccgcggccg
     4201 attgtgcgct tgccatgtat cacgatcagg gcctccccgt gctcaagtac gcgagcttcg
     4261 gcaggggtat caacgtcacg ctcggcctgc ccatgatccg cacctcggtc gaccacggta
     4321 ccgcgatcga gctcgccggc accggtcagg ccgagcccgg cagcctcatc gaggcgatcg
     4381 aagcggcgat cgaaatggcg caagccggga tgcggacggc gacctagaca gtggcgaccg
     4441 gtcggcatgt cccgcggaaa agattcggtc agcactttct cgtcgaccgg gggatcatag
     4501 agcgcatcgt ttccgcgatc gatccgcggc cgggcgatca catcgtcgaa atcggccccg
     4561 ggctcggagc gctgaccgcc ccgctgctcg agcgagtcga gcacctccat gttgtggagc
     4621 tcgatcggga tctgagcggg cgcctgcgcg agcgctatcc cgtcgaccgc ctgaccgtgc
     4681 accaagccga tgcgctgcgc ttcgacttcg cggcgctgcc tgcgccgctc agagtcgtcg
     4741 gcaacctccc ttacaatatt tccaccccgc ttctgttcca cgtcgccgcc gcggctgccc
     4801 ggtgcacaga cctccacttc atgcttcagc acgaggtcgt tgaacggatg atcgcgaatc
     4861 cgtcggaacc cgaatacggg cggctgtcgg tctcgctgca gtaccgcttc cgcctcgagc
     4921 ggctcttcac cgtcgcccct ggttcgttcc ggcctgctcc caaagttcac tccgccgttg
     4981 tgcgcctcgt gccgcgccct atggcggagc tcgatgcctt gaatgaagcc ggcttctccg
     5041 gaatcgtcgc ccgggcgttt tcgatgcggc gaaagactct gagaaacaac ctttcggggc
     5101 tgatttcccc ttctgatctc gccgaactgg gcatcgaccc gggcaagcgc gcgcaaaatc
     5161 ttccggttgc ggcttttgtc acaatcgcca accggcttgc gcagcggcgc acctagaccc
     5221 cgcaaacgcc cgctggcaaa atagtgtata gatagatgga tatgaagttt gaggcagggg
     5281 gacggcaatg ggccttcttg acgacctgaa aaaacaggcc gacttggtaa agacccagca
     5341 gattcttcag cagaatctgc agggggacaa gctcaagctg gtcgaagata agatgaaaca
     5401 gacgttccag tacgtgcatg agctgctcaa gcagctcgcg gtgctcaagc ccaccagccc
     5461 cctcgtgtat tcgatccccg gggtcgccga tttccggaat ctgctgttta ccgagtcgtt
     5521 catcgactac cggaaaaagc gcatcaacga caaggaatat ttcgacacga tgcatttctt
     5581 cgtcaagtgg gccggcccgg ataccttggt gatcgaaaaa gacatgcccc cggcgatgca
     5641 gcggatccgt gagtctctgt ggctgagcaa agtcaaattc gtcgaggagg agaagaagag
     5701 cgccaggggt ttcgtcgtcg gtgccaaatt cgtcattccc accgcagtcc tcaccgacat
     5761 cgtcatcaag gcggaccacg agcaaggcaa gctgcacttc caggcgacgc atctttttgg
     5821 cttgggggtg gaatactttt ccattcccgc gcaggaagtc agcgacgata tgctcgagga
     5881 cttcgccaag gcgctgatcg ggcagcaaag cgccatccgc aaataccgcg tcgcaggcgc
     5941 gcccgggacg tttcgctgag ctgccgcgct cgagtttccg gtttgttgga gcgtcgccga
     6001 gcctatttgc gctcgatcag ctcgatcttg tagctgtcgg gatcctcgat gaatgcgatc
     6061 actgtcgtgc cgtgcttcat aggcccggcg tcgcgagtga cctttccccc gcgcagcttc
     6121 acttcctcgc aagccttgta agcgtcgtcc actgccacgg cgatgtggcc gaaggcgctg
     6181 ccgagctcgt acttgtcgac accccagttg taggtcagct cgatcactgc ggtatcggtt
     6241 tcgtcgccgt agccgacgaa ggccagggtg aatttgccct ccggatagtc cttgcggcgg
     6301 agcaggcgca tgcctagaac ctcggtgtag aagcgcagcg accgctccag atcgccgact
     6361 cgcaacatgg tgtggagaat tcgcatcgat gatctcctct caacgctggg gacacagtct
     6421 tgccatgcgg ccctcgatcc attcctcgac cgacaggttc agcgattccg gcgtgtgggc
     6481 acgcgaatcg atcggcggcc cgacctcaac cgtcaccgtt cctggacgct tgagaaaggc
     6541 gttgcgtccc caaaacaggc cggcattgtg cgcgaccgga acgaccgacg cgccgcattt
     6601 caccgcaagc catgcgccgc cttcgtgata cttgcggcgt tcgcccggtt ttacgcgcgt
     6661 gccctccggg aagatcgcga tccagaaccc ttgcgcgagc cgttcgccgc cgagctcggc
     6721 catccgattc agcgcccgca cgccacggct gcggtcgatc gcgatcgggc tcgtgagcgc
     6781 aaggccccag ccgaagaagg ggatccacag gagctctcgc ttcaatacca gcacctgcgg
     6841 cgggaaaatg acctggaagg ccagggtttc ccaggccgat tgatgctttg ccaggatcac
     6901 gccgggagtc cgcgggaggt tctccaggcc aaccacgcgg tagtggatgc cgagcacgtt
     6961 tttcgccagc cagatcatgg cgcgcgacca gccggagatg actcgatagc gcgcaagccg
     7021 cggcaaaggg aaagtagcga gcgcgaggat cgcgtacggc ggggtgacga ggatctcggc
     7081 gatcaggaag acgagcgagc gcagaaaggt cattggcaca gcgcgtgcgc caccgcggcc
     7141 agatccggat agacgacggt cccttcggga agctgcccgg cggagcgcgt cttcgcgccc
     7201 ttgccggaaa gcaccaggat cggatgggct ccaaccgcgg ccgcggcctg gaggtcgcgc
     7261 aggctgtcgc ccacgcaggg cgcccgctcg agcgcgacat tgaggcgttc ggcgacggcg
     7321 aagaacattc ccggattggg cttgcggcag ggcgattggg actcggccgt atcggggcaa
     7381 tagaacaccg cctcgatgcg tccgcccgcc tggttcacca cccggtgcat cttggagttg
     7441 attgcattca ggcttgccat gtccataagg ccgcggccga taccggactg attggtcgcc
     7501 acgacgacac gccagccccc ctgcgtcagg cgggcgatgg catcgaggct tcccggaatc
     7561 ggccgccact cgtcgggatg cttgatgaac tcgtcgctgt cgtagttgat cacgccgtcg
     7621 cggtcgagga tgatgagttt catgctgcca gcttggagat atcggagaac cgattcatcg
     7681 ctttgcgcat ggccgtgagc agcgcgagcc ggttttcgcg aagcttcttg tcgtccgtca
     7741 tgaccatgac cgcgtcgaag aacgaatcca ccgggctctt caaagacgca aaggctttca
     7801 ggtatccagc gaaatccccg gagtcgaata gaggcttcgc tgaacgggac gccgtctgca
     7861 aggcttcgaa aagcaggatt tcggcgggct ccttgagcgc tgaagcgtcg gtatcggcaa
     7921 atgactcgcc tttgctttcc gcctgtcgca gtatgttggc gattcgcttg tttgccgccg
     7981 caagactggc ggcttctgga
//
LOCUS       DASPZD010000174         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_450707, whole genome shotgun sequence.
ACCESSION   DASPZD010000174 DASPZD010000000
VERSION     DASPZD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450707"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VEM33_03390"
     CDS             <1..1437
                     /locus_tag="VEM33_03390"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYS50899.1"
                     /translation="RTLGMFVPTQSPERAEIPAVPDLAAALKDYRGSAAVAQGEDFNP
                     TPASIEARVVRRTLPSGMKLALLPKKTRGGIVVAQLSLRWGDEQSKLNRAAACGIASG
                     MLLRGTQKNSREQLRNQFDRLKANVGVGGDGGSVETVRESFPEVLRLMAEVLRQPSFP
                     NEEFEQLRRSSLTSIDTQRSDPGALASLALARHLNPYPPEHWLYTATLDERSARLQAL
                     SLAEVRRCYADFYGASDSELAVVGDFDPEQIARLAQELFGGWKSPRPYARIPLRIAEV
                     APVDDVINTPDKANAVLRAGVLLRLRDDNADYPALLLGNYLLGGSSDSRLMRRIRENE
                     GLSYSVGSFLSADSFFERGSFGVFAIYAPQNRARVEAALSEELRKALADGFSAEEVEA
                     GKKGLLQSRQLARSSDSTVASRLVSYLVLGRTFAWEEEQERRIAAMTPQAVVEALRRH
                     IDPARLSVVKAGDFTSVAANPPGPSRAN"
     gene            complement(1523..2074)
                     /gene="greB"
                     /locus_tag="VEM33_03395"
     CDS             complement(1523..2074)
                     /gene="greB"
                     /locus_tag="VEM33_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HYS50900.1"
                     /translation="MSKAFTREGGGPEEEIEAEPRVPQGRKNYITHAGYARLRSELKA
                     LDEVERPEVVKTVAWAASNGDRSENADYLYGKKRLREIERRIRFLIKRLEIAQVVDPR
                     DQDQDRVFFGATVTYRDAGGAEHTVSIVGTDEVDPARGRVSWVSPIARALLRARAGDA
                     VTLSTPAGDERLEVLAIRYEELK"
     gene            2158..2853
                     /locus_tag="VEM33_03400"
     CDS             2158..2853
                     /locus_tag="VEM33_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HYS50901.1"
                     /translation="MKLNSRRVREIALVVLVALAATLANLPEETLERFDIDRGLLLGS
                     LALVVFIALFLYLKFQFFFLVVLLAVAANMPGEVAESLGISTLPLVIAMSTMVGFSLI
                     NYLVDLLPTGLEKNPKQQSVEGIKVLFYAIEKGNLIYAQKVLSQNFDPNLVAENGYTP
                     LMYAAARGDSKMVELLLRNGADVNMVSEEGDTAIELALRIGSQECADILKKARADQVM
                     REQATQTPEAKQE"
     gene            2955..>3342
                     /locus_tag="VEM33_03405"
     CDS             2955..>3342
                     /locus_tag="VEM33_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HYS50902.1"
                     /translation="MSKKKLAVVLAVCAAATVLGFEGGRLTAISSSQAQTAQVQRSAA
                     GALPDFSALVEQNGPAVVNISTTTAPVRTQMQIPQTPGDPGDPIQEFFRRFQIPMPQG
                     DDAIRRGVGSGFIVSADGYILTNAHVV"
BASE COUNT          578 a         1146 c         1073 g          545 t
ORIGIN      
        1 cgcacgctgg gaatgttcgt cccgacgcag tcgcccgagc gcgcggagat tccggccgtg
       61 cccgatctcg ccgccgcgct caaggactac cgcggctcgg ccgcggtcgc gcagggcgag
      121 gacttcaacc cgacccccgc gagcatcgag gcgcgcgtcg tccgcaggac gctcccaagc
      181 ggcatgaagc tcgcgctttt gccgaagaaa acgcgcggcg gcatcgtggt ggcgcagctc
      241 tctctgagat ggggcgacga gcaaagcaag ctgaaccgcg cggcggcctg cggcatcgcg
      301 agcgggatgc tgctgcgcgg cacgcagaag aattcccggg aacagctccg caatcaattc
      361 gaccggctca aggcgaacgt gggagtcggc ggcgacggag ggtccgtcga aactgtccgc
      421 gagagctttc cggaggtgct gcggctgatg gccgaggtgt tgcgccagcc gtcctttccg
      481 aatgaggagt tcgagcagct gcgccgctcg tctctcacca gcatcgatac gcagagaagc
      541 gatccgggcg cgctcgcgag cctcgcgctc gcccgccacc tgaacccgta cccgcccgag
      601 cactggctct acacggccac gctcgatgag cgcagcgccc ggctccaggc gctctccctt
      661 gccgaggtca ggcgctgcta cgccgatttc tacggcgcct ccgacagcga gctcgcggtc
      721 gtgggcgatt tcgacccgga gcagatcgcg cgcctcgccc aggaactctt cggcggctgg
      781 aaaagcccgc gcccctacgc gcgcatcccg ctgcgaatcg cggaggtcgc gccggtcgac
      841 gacgtgatca acacgccgga caaggcgaac gcggtgctgc gcgctggcgt gctccttcgg
      901 ctgcgcgacg acaacgccga ttatccggcg ctcctcctcg gcaactatct tctcggcggc
      961 tcgtccgact cgcgcctgat gcggcgcatc cgcgagaacg agggcctctc ctacagcgtc
     1021 ggctcgttcc tgtcggccga cagcttcttc gagcgcggca gtttcggggt cttcgcgatc
     1081 tacgcgccgc agaaccgcgc gcgggtcgag gcggcgctct ctgaagagct ccgcaaggcg
     1141 ctcgccgatg gtttcagcgc ggaggaagtc gaggccggaa agaagggcct gctgcaatcg
     1201 cgacagctcg cgcgctcgag cgactcgact gtcgcgagcc gcctggtttc ctacctcgtg
     1261 ctcgggcgca ccttcgcctg ggaagaggag caggagcggc ggatcgccgc catgacgccc
     1321 caggcggtcg tcgaagccct gcgccggcat atcgatccgg caagactatc cgtggtcaag
     1381 gccggggact ttacgagcgt cgcggccaac ccgccggggc cgagccgggc aaactgagag
     1441 taaaccgggg acagaccacc attttcggga taagcaggga agacccgatg ccggggaaaa
     1501 tggtggtctg tccccggttt tcttacttca attcttcgta gcgaatcgcc agaacttcga
     1561 gccgttcgtc cccggccggg gtactgagcg tcaccgcgtc gcccgcgcgc gccctgagca
     1621 gcgcccgcgc gatcggagac acccacgata cccggccgcg cgcggggtcg acttcgtcgg
     1681 tcccgacgat gctgacggtg tgctccgcgc cgcccgcatc gcgataggtg acggtggcgc
     1741 cgaagaacac gcggtcctgg tcctggtcgc gcggatcgac gacctgggcg atctccaggc
     1801 gcttgatgag gaagcggatg cggcgctcga tctcgcgcag gcgttttttc ccgtagaggt
     1861 agtcggcgtt ttccgagcgg tcgccgttgg acgccgccca ggccaccgtc ttcaccacct
     1921 ccggccgctc gacctcgtcc agcgccttca attccgatct gaggcgcgca taacccgcgt
     1981 gcgtgatgta gttcttgcgc ccctggggaa cccggggctc ggcttcgatc tcctcctcgg
     2041 gtccgccacc ctcgcgcgtg aatgccttgc tcatcgctcc atatcccaag cgtagccgcc
     2101 ctcgcggctc tactataata gcctcaaaca agcgggggcc cgtgtaggga aagcaccatg
     2161 aagctcaaca gcaggcgggt gcgggaaatc gcgctggtcg tcctggtggc gctcgcagcg
     2221 accctcgcca acctgcccga ggaaacgctc gagcgcttcg atatcgaccg gggtctgctg
     2281 ctcggctcgc tcgcgctggt cgtattcatc gcgctgttcc tgtacctcaa gttccagttc
     2341 ttcttcctgg tggtgctgct ggcggtcgcc gcgaacatgc ccggagaggt ggccgagagc
     2401 ctgggcatct cgaccctgcc gctggttatc gccatgtcca ccatggtggg cttttcgctc
     2461 atcaactact tggtcgacct gcttccgacg ggcctcgaga aaaacccgaa acaacagagc
     2521 gtcgaaggca tcaaggtgct gttctacgcc atcgagaagg gcaacctcat ctacgcgcag
     2581 aaggtcctct cccagaactt cgaccccaac ctcgtcgccg agaacggcta tacgcccctc
     2641 atgtacgccg cggcgcgcgg cgactcgaag atggtggagc tcctgctcag gaacggcgca
     2701 gacgtcaaca tggtcagcga ggaaggcgac acggcgatcg agctcgcgct gcgcatcggg
     2761 tcgcaggaat gcgccgacat cctgaagaag gcgcgtgccg accaggtgat gcgcgaacag
     2821 gccacacaga ccccggaggc gaagcaggaa tgaggtcttt tccgtaacaa gatgtgtcgg
     2881 ggccggcacg gcgggaactt caacccgcat gcgctgccca agacgcgtta ccgaaatcat
     2941 tgcaggagac ccctatgtcc aaaaagaaac tcgccgtggt gctggccgta tgcgcggctg
     3001 cgacggtgct gggcttcgag ggcggcaggc tgaccgccat atcgagctcg caggcccaga
     3061 cggcgcaagt ccagaggtcc gccgccggag cgctccccga cttcagcgcc ctggtcgagc
     3121 aaaacgggcc cgccgtcgtg aacatcagca ccaccaccgc gcccgtgcgc acccagatgc
     3181 agatacccca gacccccggc gacccgggcg atcccatcca ggaattcttc cgccgcttcc
     3241 agattcccat gccccagggc gacgacgcga ttcgcagagg ggtcggctcg ggtttcatcg
     3301 tgagcgccga cggctacatc ctgaccaacg cgcacgtcgt cg
//
LOCUS       DASPZD010000175         5490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_745547, whole genome shotgun sequence.
ACCESSION   DASPZD010000175 DASPZD010000000
VERSION     DASPZD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745547"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1268)
                     /locus_tag="VEM33_03410"
     CDS             complement(99..1268)
                     /locus_tag="VEM33_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYS50903.1"
                     /translation="MRAKPLSGIRVLDLTRLLPGPMATLHLADMGADVIKIEDTEAGD
                     YSRSMGRVRGVPPMSDFFRLVNRNKRAIRLDLKNPKGCEVFLRLAKGADVVVEGFRPG
                     VMAKLGVGYDALAAVNARLVYCSITGYGQDGPYAGRAGHDVNYIGYAGVGDQIGTAET
                     PVVPNFQIADLLGGALAPVMGILAALIDARSSGRGRHVDVAMTDAVFAHAIFPLLGFL
                     EHGRTPPRASGMLDGGLPCYNVYCARDGRWMAVGALERKFWETLCDILGCPELKGKHI
                     VYGEEARQVKERLAAAFGSRTQREWSEVFARADCCVSPILTVDEALENEQLRARDMVI
                     VEEGLTQLAPPLKLSEFDFAIVRPAPRSGEHTDEVLREAGFSDAEIAALRRSAVI"
     gene            complement(1269..2072)
                     /locus_tag="VEM33_03415"
     CDS             complement(1269..2072)
                     /locus_tag="VEM33_03415"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50904.1"
                     /translation="MTRVGPLLVLSLVLAAPLGAGAEEIVTVSGRADATQSYLLLHDA
                     PPPVAVAVLFPGGEGLLGLRVEGGAVKFRQHGNFLVRTRALMRDRQVGIAIVDSPSDQ
                     QRAGMDDGFRTGRAHVADVAAVVKDLKQRFPGAKIFLIGTSRGTISAAYAGRALNGAI
                     DGVILTSTVFYGPRRGGGIGIASFDFAAIKAPLLFVHHRYDGCGSCPYHAAENLGRTY
                     PLITVMGGKPAESGPCEPFAAHGYFGKEAETVAAIRSWMLGQPFPKTVD"
     gene            complement(2065..3303)
                     /locus_tag="VEM33_03420"
     CDS             complement(2065..3303)
                     /locus_tag="VEM33_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYS50905.1"
                     /translation="MPEFYDALETRDPESRERTLMDALPRQIAHAKDKAPGWARILSE
                     VDPPAVTGRAALAKLPVTRKPDLAALQKAEPPFGGLNATPLTQLGRIFVSPGPIYDPV
                     GRGHDWGRAARALFAAGFRSGEVALNTFAYHFTPAGSMMEAGALALGCAVVPGGTGQT
                     EMQVAAIASLRASAYIGTPSFLKLIVEKADELKTDIGTLRKAAVGAEYLPAPLRAAMG
                     ERGIRVLQSYASADLGLIAYESEALEGMILDEALILEIVRPGTGDPVPEGEVGEVVVT
                     SFNPDYPLMRFGTGDLSAILPGQSPCGRTNVRIRGWMGRADQTTKVKGMFVQPSQVAE
                     IARRHKEILKARLAVDNPGGTDRMTLQCEVRGTPPAGLAQAIEDSIRSITKLRGEVAF
                     KKPGELPNDGKVIEDAKKYD"
     gene            3473..4918
                     /locus_tag="VEM33_03425"
     CDS             3473..4918
                     /locus_tag="VEM33_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HYS50906.1"
                     /translation="MDSKGIGEDWLSVWIGLSIFVLALGSLGGSDLLGWVVTTGVWTD
                     LSKALNPVSKAYSALGGVGALIATYVALIVAMTAGAAALKADLKRFALGFTAVFWISY
                     LSWIAGSYANFAVTTPADMQRFGISWSLKLTNEGGFILALVAGLVVGNFFPGFAAWMK
                     EAVRPELYIKTAIVLLGGFLGIMAATQPVLAKSILFRGLAAIIEAYLIYWAVVYFVAR
                     KWFGFSREWSAPLASGISICGVSAAIATGSAIRARPIVPIMVASLVVIFAVVELLILP
                     FAANAFLAHEPMVAGAWLGLAVKTDGAAVASGAIAESLILATNTAEGINYAKGWILGT
                     TATVKIFIDIFIGIWAFILAYIWTNHIEVRSAGDKAKIGEIWERFPKFILGYVLTFVV
                     VLLLAYGTAPATATKIRAAMGEANTFRVIFFVMTFFTIGVMSNFRRLWEEGIGKLAAV
                     YVVCLFGFVIWVGLVISWVFFAGVKPPLAAG"
     gene            4933..5073
                     /locus_tag="VEM33_03430"
     CDS             4933..5073
                     /locus_tag="VEM33_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50907.1"
                     /translation="MEQPKVGEEMRKMPVEPLLPVEKKLIGWSLGMGLVLLVVLVFAT
                     RS"
     gene            complement(5084..>5490)
                     /locus_tag="VEM33_03435"
     CDS             complement(5084..>5490)
                     /locus_tag="VEM33_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50908.1"
                     /translation="VQSVLAGDTQVTFATAPSVMPHVQTGKLRGLAVTTRGRSPLVEG
                     LPGMEEAGLANYDVSIWYGFFAPKATPRAIVERLFEATTQALQDTKLKAVLAPAGTEA
                     VASKSPADFGAFLRRDAEAIARVIRETGVKFE"
BASE COUNT          924 a         1805 c         1763 g          998 t
ORIGIN      
        1 ttgcgcgagc gcggcgtcgg aacagaaaag cagcacggcg aacagcgccg gagcaagggt
       61 cggcgcgtgc cgcgttctga gggtcggcgc cgccacgttc agatcaccgc gcttctgcgc
      121 aatgcggcga tttcggcatc gctaaatccg gcttcgcgca ggacttcatc ggtgtgctcg
      181 cccgaccggg gcgccgggcg gacgatcgcg aaatcgaatt cgctgagctt gagcggaggc
      241 gcgagctgcg tcaacccttc ctcgacgata accatgtcgc gggcgcgcaa ctgctcgttc
      301 tccagcgcct cgtctacggt cagaatcggg ctcacgcagc aatccgcgcg ggcgaagacc
      361 tccgaccatt cacgctgcgt gcgcgatcca aaggccgccg ccaggcgttc tttcacctgc
      421 ctcgcttcct cgccgtatac gatgtgcttt cctttcaatt cggggcatcc gagtatatcg
      481 cacagcgttt cccagaattt tcgctcgagg gcccccaccg ccatccagcg gccgtcccgc
      541 gcgcagtaga cgttgtagca gggcagcccc ccgtcgagca tgcccgaggc gcggggcggc
      601 gtccttccgt gttcgagaaa tcccagaagg ggaaagatcg cgtgcgcaaa caccgcgtcg
      661 gtcatggcga cgtccacgtg ccggccgcgc ccggatgatc tcgcgtcgat cagcgctgcg
      721 aggattccca tcaccggcgc gagcgccccg cccagcaggt cggcgatctg gaagttcggc
      781 acgaccggcg tctccgccgt tccgatctgg tcgccgacgc cggcgtagcc gatgtagttg
      841 acatcgtggc cggcgcgacc tgcgtaaggc ccgtcctgcc cgtagccggt gatcgagcaa
      901 tacaccaggc gcgcgttcac tgcggcgagc gcatcgtagc cgacgccgag cttcgccatg
      961 acgccgggac ggaagccttc cacgacgacg tccgctcctt tcgcgagccg caggaacact
     1021 tcgcaccctt tcggattttt cagatcgagc cgtatcgcgc gcttgttgcg gttgacgagc
     1081 cggaaaaaat cagacatggg aggaaccccg cgcacgcgcc ccatcgagcg ggaatagtcc
     1141 cctgcctccg tgtcctcgat cttgatcacg tccgccccca tgtcggcgag gtgcagcgtc
     1201 gccatcggac cgggaaggag ccgcgtcagg tcgaggacgc ggatgccgga aagcggtttc
     1261 gcgcgcatct agtcgaccgt cttcggaaaa ggctgcccca gcatccagct cctgattgcg
     1321 gcgaccgtct cagcttcctt gccgaagtag ccgtgcgcgg cgaacggctc gcagggccca
     1381 gactcggcag gcttgccgcc catcaccgtt atcaggggat aggtcctgcc aagattctcc
     1441 gcggcatggt agggacagga cccgcagccg tcgtagcggt gatgaacgaa gaggagcggc
     1501 gccttgatcg cggcgaaatc gaagctggcg atgccgatgc cgccgccgcg tctcgggccg
     1561 tagaacaccg tggaagtgag gatcacgccg tcgattgcgc cgttcaatgc gcggccggca
     1621 taggcggcgg aaatcgtgcc gcggctcgtg ccgatcagga aaatcttcgc acccggaaag
     1681 cgctgcttca gatccttgac caccgcggcg acgtccgcga cgtgcgcgcg gccggtgcgg
     1741 aagccgtcgt ccatcccggc gcgctgctgg tccgagggcg aatcgacgat ggcgatcccc
     1801 acctgcctgt cccgcatgag cgcacgcgtg cgcaccagaa aattcccgtg ctgccggaat
     1861 ttgactgcgc cgccctcgac acgcaggccg agcaggccct cgccgccggg aaagagcacc
     1921 gccaccgcca cgggcggtgg cgcatcgtgc agcagcagat acgactgcgt cgcgtcggcc
     1981 cggcccgaga ccgtgacgat ttcctctgca ccggcgccaa gcggcgctgc gagcacgagc
     2041 gataagacaa ggagcggccc aaccctagtc atattttttc gcgtcctcga tcaccttgcc
     2101 gtcgttcggc agctcgcccg gctttttgaa agcaacttcg ccgcgcagct tggtgatgct
     2161 gcggatgctg tcttctatcg cctgcgcgag tcccgccggc ggcgtgccgc gcacttcgca
     2221 ttgcagggtc atgcggtcgg ttccgcccgg attatccacg gcgaggcgcg ccttcaggat
     2281 ctccttgtgt cgccttgcga tttcggcgac ctgcgatggc tgcacgaaca ttcccttcac
     2341 cttggtggtc tggtccgcgc ggcccatcca cccgcggatc cgaacattgg tgcgtccaca
     2401 ggggctttgc cccggcaata tcgccgacaa gtcaccggtc ccgaatcgca tcagcgggta
     2461 atccggattg aagctggtga cgaccacttc gcccacttcg ccttccggca cgggatcgcc
     2521 tgtgccgggg cgcacgatct ccaggatcag cgcttcgtct aggatcatgc cctcgagcgc
     2581 ttcggattcg taggcaatga ggcccaggtc cgcggaggcg tacgactgca gcacgcggat
     2641 gccgcgttcg cccatcgcgg cgcgcagcgg cgcgggcaaa tactccgcgc ccaccgcggc
     2701 tttcctcaga gtgccgatgt cagtcttcag ctcatcggcc ttctcgacga tgagcttcag
     2761 aaaggaaggc gtcccgatgt aggcgctcgc gcgcaagctc gcgatcgctg cgacctgcat
     2821 ttccgtctgc cccgtgccgc ccggcacgac cgcacagccg agcgcgagcg cgcccgcctc
     2881 catcatcgag cccgcaggtg tgaaatggta ggcaaaggtg ttgagcgcga cctcgccgga
     2941 acgaaagccc gccgcaaaga gcgcgcgcgc cgcgcgcccc cagtcgtggc cgcgccctac
     3001 aggatcgtag atcggtccgg gcgagacgaa aatcctgccg agttgcgtga gcggcgtcgc
     3061 gttaaggccg ccaaaaggcg gttcggcctt ttgcagcgca gcgaggtcgg gcttgcgcgt
     3121 gactggaagc tttgcgagcg ccgcgcggcc ggtgacggca gggggatcga cttccgaaag
     3181 aatgcgcgcc cagccgggcg ccttgtcttt ggcatgcgcg atctgccggg ggagcgcgtc
     3241 catcaacgtg cgttcgcgcg actccggatc gcgggtttcg agagcgtcgt agaactcggg
     3301 catcgatgag caatgcgttc gcggcagggc aacatagcac aaaggcaaaa gcagcatggg
     3361 ttattaagaa tgaatataaa attcgttcct gacggggagt cgagccggta agtttccgat
     3421 tttccgtccg cggcacagta caatcgggcg gaatctcctc cgggaggaaa ccatggactc
     3481 caaaggcatc ggcgaagact ggctgtcggt ctggatcggc ttgtcgattt tcgttctcgc
     3541 gctcggctca ctggggggat cggatctcct gggctgggtc gtcaccacgg gggtctggac
     3601 cgatctttcg aaagccctca accctgtctc caaggcctat tcggccttag gcggagtggg
     3661 cgccctgatc gccacctacg tcgccctgat cgtcgcgatg acggcgggcg cggccgcgct
     3721 caaagccgat ctcaagcgct tcgcgctcgg cttcaccgcg gtgttctgga tcagctacct
     3781 ctcctggatc gcggggagct acgcgaattt cgccgtgacc acgcccgcag acatgcagag
     3841 attcggcatc agctggtcgc tgaagctcac caacgagggc ggattcatcc tcgcgctcgt
     3901 cgccggcctc gtcgtcggga acttctttcc gggcttcgcc gcgtggatga aggaggccgt
     3961 gcggccggag ctgtacatca agactgcgat cgttctcctc ggcggcttcc tcggcatcat
     4021 ggcggccacg cagccggtgc tggcgaagtc gatcctgttc cggggcctcg ccgccatcat
     4081 cgaggcgtac ctgatctact gggcggtggt gtacttcgtt gcgcgcaaat ggttcggctt
     4141 cagccgcgag tggtcggcac cgctcgcatc gggaatctcg atctgcggcg tgtcggcggc
     4201 gatcgccacc ggcagcgcga tccgcgcccg gcccatcgtc ccgatcatgg tggcttcgct
     4261 cgtggtgatt ttcgccgtgg tcgagctgct cattctgccg tttgccgcaa acgctttcct
     4321 cgcgcacgag ccgatggtcg cgggcgcctg gttggggctc gcggtgaaga ccgacggcgc
     4381 ggcggtggcg agcggggcga tcgcggaatc gctgatcctc gccaccaata cagccgaggg
     4441 catcaactac gcaaagggct ggatactcgg gaccaccgcg acagtgaaga tcttcatcga
     4501 catcttcatc ggcatctggg cctttatcct cgcttacatc tggaccaacc acatcgaagt
     4561 ccgctccgcc ggcgacaagg cgaagatcgg cgagatctgg gagcggttcc cgaaattcat
     4621 cctcggctac gtgctgactt tcgtagtcgt cctgctcctc gcctacggca cagcaccggc
     4681 gaccgccacc aagatccgcg cggcgatggg cgaggcgaac accttccgcg tgatcttttt
     4741 cgtcatgact ttcttcacca tcggagtaat gtccaacttc aggagactct gggaggaagg
     4801 catcggcaag ctcgccgcgg tctacgtggt ttgtctcttc ggtttcgtca tctgggtggg
     4861 tctggtgatc tcctgggtgt tcttcgcggg ggtgaagccg ccgctcgcag caggttgaaa
     4921 gggagaaaga tcatggaaca acccaaagtc ggcgaggaaa tgcgcaagat gccggtcgag
     4981 ccgctgctcc ccgtcgagaa gaagctgatc ggctggagcc tggggatggg cctggtgcta
     5041 ctggtggtcc ttgtcttcgc gacgcgaagc tgaacgcggc cggttactcg aacttcactc
     5101 ccgtctcgcg gatcacgcgc gcgatggctt ccgcatcccg gcggaggaac gcgccgaagt
     5161 ccgcgggcga cttggacgcg actgcctcgg tccccgcggg cgcgaggacg gccttcagct
     5221 tcgtatcctg aagcgcctgc gtcgtcgctt cgaacagccg ctcgacgatc gcccgcggcg
     5281 tcgctttcgg cgcgaaaaat ccgtaccaga tggaaacgtc gtagttcgcg aggcccgctt
     5341 cctccattcc cggcaagcct tcgaccagcg gcgaacgccc gcgcgtcgtg acggcgaggc
     5401 cgcgcagttt gcctgtctgc acgtgcggca tcaccgaagg cgcggtggcg aaggtcacct
     5461 gggtgtcgcc ggcgagcacc gactgcaccg
//
LOCUS       DASPZD010000176         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_382959, whole genome shotgun sequence.
ACCESSION   DASPZD010000176 DASPZD010000000
VERSION     DASPZD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382959"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VEM33_03440"
     CDS             complement(<1..271)
                     /locus_tag="VEM33_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYS50909.1"
                     /translation="MIRIGVDIGGSKMEIAAIDESGAELLRRRESAPRGGYAQAVEVI
                     AQFVLEAEREVGAPCTVGIGTPGAVSRGSGVLKNAYASALNGMPVK"
     gene            complement(300..2087)
                     /locus_tag="VEM33_03445"
     CDS             complement(300..2087)
                     /locus_tag="VEM33_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYS50910.1"
                     /translation="MTSLDLALIGNSSVAALVDANGTIVWSCLPRFDSDAVFCSLLRD
                     GRGADDHGFFEIELADFARAEQAYIAHTPILKTRLFDRHGGGVEITDLAPRFEQFGRL
                     FCPVMLMRQVRPISGSPRIRMRIRPACEYGARRPAITWGSNHVRYVMPEMVLRLTSDY
                     SITAILEETPIVLREPVSVVLGPDETLQGSVGEVFRRFLDDTAAFWRAWVRNLGIPYE
                     WQENIIRAAITLKLNAYEDTGAIVAAVSTSIPESGGSGRNWDYRYCWLRDAYFVVNAL
                     NRMNATRPMERYLDYIINIATSTNGRDLQPVYAISGKAGIEEREIGSLPGYRGMGPVR
                     VGNQAYSQVQHDVFGAAILAAAHVFFDARLALRNDESLLRQLEQLGERAAALFDQPDA
                     GLWELRGTKRVHTFSSVMCWAGCDRLAKIAARIGLADRARAWRARADTMHSAICDRAW
                     NAKRKTFAATFGGEDLDASLLLLHELNFLRADDPRFASTVQAVERELRRGEYLFRYSV
                     PDDFGVPENAFVVCSFWYIDALSALGRKEEARALFQSILARCNRHGLLSEHIDPRTGE
                     LWGNFPQTYSMVGMINSAMRLSIPWDEAY"
     gene            complement(2084..2818)
                     /gene="otsB"
                     /locus_tag="VEM33_03450"
     CDS             complement(2084..2818)
                     /gene="otsB"
                     /locus_tag="VEM33_03450"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HYS50911.1"
                     /translation="MPRLPEDAGFFLDIDGTLLDIAERPQLVRIDDDLGHLLATIRDA
                     SGGAVALISGRSVAEIDRLFGRNFCAAGQHGAERRDAAGRMHQHRVPLAGLRKALKRL
                     RAMAAEHPALVLEDKGMNLAVHYRSAPELGATVREALRRLVEELGDDFELQSGKMVME
                     IKPSGKDKGTAIEEFLAEAPFRDRLPVFIGDDLTDEFGFELINRVGGCSVKVGEGSSA
                     AHWRLPNADAVRAWLKRFVERGREKP"
     gene            complement(2936..5182)
                     /locus_tag="VEM33_03455"
     CDS             complement(2936..5182)
                     /locus_tag="VEM33_03455"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HYS50912.1"
                     /translation="MRLSLRFILPLALTLAAIAYAVIPLVDRFTLQWFVRDLDIRTSL
                     IANTIQDPLGDLVREGSRTKVMRFFDRIMLDERLFAVGFCDPSRRMIYKTAVFPASIT
                     CAGEDRDALVSESRLVNLQRGPIHVAIHRVESEGTAYGYLMLVHDMSFVQRRSDDTKK
                     YIFYLFAAIGGIVSLVTVVIAEISWRGWVAGLKAVIRGEAFGRGETEVPLRELRPVAR
                     DLQALIRDLESERKARDESQINWSAETLRAILRNDLKGEEVLIVSNREPYIHMRRNER
                     IEVQRPASGLVTALEPVMRACSGSWIAHGSGNADRETADASDHVMVPPERPAYRIRRV
                     WLSKEEEAGYYYGFSNSGLWPLCHIAHTRPIFRTADWNAYVTVNRRFAETVVREAKTA
                     DPIVLVQDYHFALLPRMIRERLPEATVITFWHIPWPNAEAFGICPWREAILDGILGSS
                     ILGFHTQFHCNNFIDAVDRYVEARIDRETYTISYGGDQTAVRRYPISIEWPLNSKTMQ
                     KPTGECRADVRKRNGLAADALIGVGVDRLDYTKGILERFLAVERFLELEPEWIGKFVF
                     VQIAAPSRSSIDEYQNYDARVRALARRINDKFSNKHHDPILLKIEHYDPDDVYEYYRA
                     SELCFVSSLHDGMNLVAKEFVASRDDERGVLVLSQFTGASRELPEALIVNPYNVDQCA
                     GALKAALTMPPAEQRDRMRSMRGLIQEFNVYRWAGRMLLDAGQMRMRSRLFARADRGR
                     KPVPLRSV"
     gene            5352..>5562
                     /locus_tag="VEM33_03460"
     CDS             5352..>5562
                     /locus_tag="VEM33_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50913.1"
                     /translation="MALITSAPAWAAEGPAVLGVPVDFIVFGVTLLGVALFHRHTLGV
                     AVAGLALVTSYKLGFTGFKTGNGIPG"
BASE COUNT          899 a         1842 c         1863 g          958 t
ORIGIN      
        1 gtttgaccgg catcccgttc agcgcgctcg cataggcgtt cttgagcact cccgatccgc
       61 gcgacactgc gcccggcgtg ccgatgccca ccgtgcaggg cgcgccgacc tcgcgctcgg
      121 cttcgagaac gaattgcgcg atcacctcga ccgcctgcgc atagcccccg cggggcgcgg
      181 actcgcggcg ccgcagcagc tccgcgcccg attcgtcgat cgcggcgatc tccatcttgc
      241 tgccgccgat gtccacgccg atgcggatca tttcggccgc gagtcgaccg cgagggaatt
      301 caataagcct cgtcccaggg gatgctgaga cgcattgcgg aattgatcat gccgaccatg
      361 ctgtaggtct gcgggaaatt cccccacaat tccccggtgc gcgggtcgat atgctctgag
      421 agcagaccgt gtcggttgca gcgggcgagt atgctctgga acagcgcgcg ggcctcttcc
      481 ttgcgtccga gcgccgagag cgcgtcgatg taccagaaag agcagaccac gaaggcgttc
      541 tcggggacgc cgaagtcgtc cggcacgctg tagcggaata ggtactcgcc gcgcctcagc
      601 tcccgttcga ccgcctggac cgtcgaggca aagcgcgggt cgtcggcgcg caggaaattg
      661 agctcgtgaa ggaggagcag gctcgcgtcc aggtcctcgc cgccgaaagt cgcggcgaaa
      721 gtcttgcgct tcgcgttcca ggcacggtcg cagatcgccg aatgcatggt gtcggcgcgc
      781 gcgcgccacg cgcgcgcgcg gtccgcgagc ccgatgcgcg cggcgatctt cgccaggcgg
      841 tcgcacccgg cccagcacat gacgctggag aaggtgtgca cgcgcttcgt cccgcgcagc
      901 tcccacaggc cggcgtcggg ctggtcgaag agggccgcgg cccgttcgcc gagctgctcg
      961 agctgccgca ggagcgactc gtcgttgcgc aaggcgagcc gcgcgtcgaa gaacacgtgc
     1021 gccgcggcga ggatcgccgc gccgaagacg tcgtgctgga cctgggaata agcctggttg
     1081 ccgaccctca ccggccccat gccgcggtag ccgggcagcg atccgatctc gcgctcctcg
     1141 atccccgcct tgccgctgat cgcatagacc ggctgcaggt cgcggccgtt ggtgctcgtg
     1201 gcgatattga tgatgtagtc gaggtagcgc tccatcggtc gcgtcgcgtt catccggttg
     1261 agcgcattca cgacgaagta cgcgtcgcgc agccagcagt agcggtaatc ccagttgcgg
     1321 ccgctgccgc ccgactccgg gatcgaggtg ctcaccgccg ccacgatcgc gccggtatcc
     1381 tcgtaggcgt tgagcttcag ggtgatggcc gcgcggatga tgttctcctg ccattcgtac
     1441 gggatgccga gattgcgcac ccaggcccgc cagaaggcgg cggtgtcgtc gaggaacctg
     1501 cgaaacactt ccccgacgga gccctgcagg gtctcgtccg gcccgagcac gaccgacacc
     1561 ggctcccgca gcacgatggg cgtttcctcg aggatcgcgg tgatcgagta gtcggaggtc
     1621 aaccggagga ccatctcggg catgacgtag cgcacgtgat tgctgcccca ggtgatcgcg
     1681 gggcggcgcg ctccgtactc gcacgcgggc cgaatgcgca tgcggatgcg cgggctgccc
     1741 gagatcgggc gcacctggcg catgagcatc acggggcaga agagcctgcc gaactgctcg
     1801 aatcgcgggg cgagatcggt gatttcgacg ccgccgccgt gacggtcgaa aagccgcgtc
     1861 ttgaggatcg gcgtgtgcgc gatgtatgcc tgctcggcgc gggcgaaatc ggcgagctcg
     1921 atctcgaaaa acccgtggtc gtctgcaccg cgcccgtcgc gcaggaggga gcaaaacacg
     1981 gcgtcgctgt cgaagcgcgg caggcaggac cagacgatgg tcccgttcgc atcgacgagc
     2041 gccgcgacgc tcgagttgcc gatcagggcg agatcgaggc tggtcacggc ttctcccgcc
     2101 cgcgctcgac gaagcgcttg agccacgccc ggactgcgtc cgcgttcggc agccgccaat
     2161 gcgcggccga gctgccttcg cccaccttga ccgagcagcc tccgacccgg ttgatcaact
     2221 cgaagccgaa ttcgtcggtg agatcgtcgc cgatgaacac cgggaggcgg tccctgaacg
     2281 gagcctcggc gaggaactcc tcgatcgccg tgcccttgtc ctttccgctc ggcttgattt
     2341 ccatcaccat ttttcccgat tgcagctcga agtcgtcgcc gagctcctcg accagccggc
     2401 ggagggcttc gcgcaccgtc gcgccgagct ccggcgccga gcggtagtgg accgcgagat
     2461 tcatgccctt gtcctcgagc accagcgcgg ggtgctcggc ggccatcgcc ctcaggcgct
     2521 tcagcgcctt gcgaagtccc gcgagcggga cgcggtgctg gtgcatcctt cccgcggcgt
     2581 cgcggcgctc cgctccgtgc tgtccggcgg cacagaaatt cctgccgaag agccggtcga
     2641 tttccgcgac cgaacgcccg ctgatgagcg cgacagcccc gcccgaggcg tcgcgtatcg
     2701 tcgcgagcag gtgcccgaga tcgtcgtcga tccgcacgag ctgcggcctt tcggcgatgt
     2761 ccagaagcgt cccgtcgatg tccaggaaga atcccgcgtc ctccggcagt cgcggcacgg
     2821 ttccgagggg gaagccggcg tccgcagccg cgccgtcctg gcgcggctgc cttcggggtt
     2881 ccacgggggc cacgtcgcgg ctccggctcc tggagttcgg catcggcacc cgtgatcaga
     2941 cgctgcgcag cggaacgggc ttgcgcccgc ggtccgcgcg ggcgaacagg cgcgagcgca
     3001 tgcgcatctg gcccgcgtcg agcagcatgc gcccggccca gcgatacacg ttgaattcct
     3061 gtatgaggcc gcgcatgctg cgcatccggt cgcgttgctc cgcgggcggc atcgtgagcg
     3121 ccgccttcag ggcgcccgcg cattgatcga cgttgtaggg attgacgatc agggcttccg
     3181 gcagctcccg cgacgccccc gtgaactggc tgagaacgag caccccgcgc tcgtcgtcgc
     3241 gcgaggccac gaactccttg gcgacgagat tcatgccgtc atgcaggctg ctcacgaaac
     3301 acagctccga cgcccggtag tactcgtaga cgtcgtcggg gtcgtaatgc tcgatcttga
     3361 gcaggatcgg atcgtggtgc ttgttcgaga atttgtcgtt gatccggcgc gcgagcgcac
     3421 gcacgcgcgc gtcgtagttc tgatactcgt cgatgctgga gcgcgaaggc gccgcgatct
     3481 ggacgaacac gaacttgccg atccattcgg gctctagctc gagaaagcgc tcgaccgcga
     3541 ggaatcgctc gagtatcccc ttggtatagt cgaggcggtc cacgccgacg ccgatcagcg
     3601 cgtccgcggc gaggccgttc ctcttgcgca cgtcagcgcg gcattccccc gtgggttttt
     3661 gcatggtctt cgaattgagc ggccattcta tcgagatggg atagcgtcgc accgcggtct
     3721 ggtccccgcc gtaggatatg gtgtaggttt cgcgatcgat tcgcgcctcg acgtaacggt
     3781 ccaccgcgtc gatgaaattg ttgcagtgga actgggtgtg aaatcccaga atgctgctgc
     3841 ccaggatccc gtcgaggatc gcctcgcgcc acgggcagat tccgaaggcc tcggcgttgg
     3901 gccagggaat gtgccagaac gtgatgacgg tcgcctccgg cagcctctcc cggatcatgc
     3961 ggggcagcag cgcgaagtga tagtcctgga ccaggacgat gggatctgcg gtcttcgcct
     4021 cgcgcaccac cgtttcggcg aaccggcggt tgacggtgac gtaggcgttc cagtccgcgg
     4081 tgcgaaagat cgggcgggtg tgcgcgatgt ggcacaaggg ccacaggccg ctgttcgaga
     4141 acccgtagta gtagccggcc tcctcctcct tggagagcca cacccgcctg atccggtagg
     4201 ccggccgctc gggcggcacc atcacgtggt cgctagcatc cgccgtttct cggtcggcgt
     4261 tgccgctgcc gtgcgcgatc cacgatccgg aacatgcccg catcaccggc tcgagcgcgg
     4321 tcacgaggcc gctcgcgggg cgctgcacct cgatgcgctc gttgcgccgc atgtggatgt
     4381 agggctcgcg gttggaaacg atgagtactt cctctccctt gaggtcgttg cgcagtattg
     4441 cccggagcgt ttcggccgac cagttgatct ggctctcgtc gcgcgccttg cgctcagatt
     4501 cgagatcgcg gatcagcgcc tggagatcgc gtgccaccgg ccgcaattcg cgcaggggca
     4561 cttccgtctc gccgcggccg aacgcttcgc cccggatcac ggccttgagg cccgcgaccc
     4621 agccgcgcca gctgatttcc gcgatcacca cggtcaccag cgaaacgatc ccgccgatcg
     4681 cggcaaagag atagaagatg tatttcttcg tgtcgtcgct gcgccgctgc acgaagctca
     4741 tgtcgtgcac cagcatcagg tagccgtagg ccgtgccctc ggattcgacg cggtggatcg
     4801 ccacgtggat cgggccgcgc tggaggttga ccaggcggct ctcgctgacg agcgcatcgc
     4861 ggtcctcgcc agcgcaggtg atcgaagcgg gaaacacggc cgtcttgtag atcattctgc
     4921 gagacggatc gcagaaaccg accgcaaaca ggcgctcgtc cagcatgatc cggtcgaaaa
     4981 agcgcatgac cttcgttcgc gagccctccc ggacgaggtc ccccagcggg tcctgaatcg
     5041 tgttcgcgat gagcgaagtg cggatatcga ggtcgcgcac gaaccactgg agcgtgaacc
     5101 tgtcgacgag cggtatgacc gcgtacgcga tggctgcgag cgtgagggcg aggggaagga
     5161 tgaaccggag cgacagtctc atggggactc ctgtcgggtt tctgggtcat gcggaaaacc
     5221 gaacgcggaa gcaatgtaac acgttcggcc gcgccatacg accctagaaa aaatacccgg
     5281 ggcggatgct acaatcggcg catcgaccgg gaggaacgct gcagcctgca cgcagaatcg
     5341 ttgccggcgc catggcgctg atcacgtccg cgcccgcctg ggccgccgag gggccggccg
     5401 tgctgggcgt ccccgtggat ttcatcgtct tcggcgtcac gcttctcggc gtggcgctgt
     5461 ttcaccgcca tacgctcggt gtcgccgttg ccggcctcgc tctcgtcact tcgtacaagc
     5521 tcggcttcac cgggttcaag acgggaaacg gcattccagg ac
//
LOCUS       DASPZD010000177         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1707638, whole genome shotgun sequence.
ACCESSION   DASPZD010000177 DASPZD010000000
VERSION     DASPZD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707638"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VEM33_03465"
     CDS             <1..667
                     /locus_tag="VEM33_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HYS50914.1"
                     /translation="AGIDFHIGSQLTEIGPFVAALDKALEFIDALGATGIRLQHIDLG
                     GGLGIRYRDEKPPTLKDYLGQLFARIGNRKLKIFFEPGRSLVGNAGLLLTRVEYLKSG
                     AAKNFAVTDAAMNDLLRPALYDAWHEVLPVMRRSGPAQRYDIVGPVCESADFLARERA
                     LTVAAGDLLAIMSAGAYGMTMSSNYNSRPRPPEVMVDGKTTHLVRDRERVENLFASER
                     LLP"
     gene            781..1926
                     /locus_tag="VEM33_03470"
     CDS             781..1926
                     /locus_tag="VEM33_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYS50915.1"
                     /translation="MRSHVAAATAKRLFVLGGVGLLVVAVVLLAYFGTEIRANEPKAS
                     KGPAAIPVSVAPVLQEVVPLRLLAIGNVEAYATVSVKARVDGQIVEVGFKEGDEVPRS
                     RVLFKIDPRPYEAALRQAEANFLRDTAQKNQARSQERRYQELLAKNFVSKEAYAQIRT
                     NADTAEAVAQGSRAALDNAALNLEYCTIRSPIDGYPGKIQIQMGNLVKANDTLPLVVI
                     NQVHPIYVNFSVPEQQLRAVRGYMSSGPLAVEALAPGSDQPAASGTLVFVDNAVDPST
                     GTIKLRAQFQNGENALWPGQFVNVRIKLYDQKDALVVPSKSVQTGPSGQYVFVVKPDM
                     TTELRKVTVARTEGDNAVIASGLEKGERVVTQGQLRLGAGAKVEVKS"
     gene            1937..>3637
                     /locus_tag="VEM33_03475"
     CDS             1937..>3637
                     /locus_tag="VEM33_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYS50916.1"
                     /translation="MNLSELFVRRPVMTVLVMAGILIFGLASYRLLPVSSLPNVDFPT
                     IQVSAQLPGASPETMAAAVATPLEKQFSTIAGIDQMTSLSGQGITSVTIQFTLDRDID
                     AAAQDVNSAIAAATKQLPPTMSTPPSFRKVNPADAPIFFLTLTSETLPLSTVNEYAET
                     FLAQRISTISGVAQVQVQGQQKYAVRVQVDPNALAARGVGINEVEQAVANANVNLPTG
                     TLYGKDRMFAIQATGQLYEAAAFRPIIVTYRNGAPIRLSELGRVIDSVENDKLAAWFK
                     DQRGIILAVYRQPGTNTIEVVDAVKKLLPTFRVEVPAGVSINVMYDRSTTIRESVEDV
                     RFSLILALALVVMVIFMFLRNIPATVIPAVALPMSIIGTFSLMYLFGYSLDNISLMAL
                     TLCVGFVVDDAIVMLENISRHIEMGKSPLQATLDGSKEVSFTILSMTISLAAVFIPVL
                     FMGGILGRLLHEFAVTIIVAVLISGFVSLTLTPMMCSRILKPRAEVKHGRLFLASERA
                     FDAVRNAYGHTLNWALRRQRFTMGVFLAIVVATGWLYVKMPKGFLPSEDAGQLFCFIE
                     A"
BASE COUNT          680 a         1178 c         1094 g          685 t
ORIGIN      
        1 cgccggcatc gatttccaca tcggttcgca gctgaccgag atcgggccct tcgtcgcggc
       61 gctcgacaaa gccctcgaat tcatcgacgc gctgggcgca accgggattc ggctgcagca
      121 catcgatctc ggcggtgggc tcggaattcg ttaccgcgac gaaaaaccgc cgactctgaa
      181 ggactacctc ggacagttat tcgcacgcat cggcaaccgc aagctgaaga ttttcttcga
      241 gccgggacgc tcgcttgtcg gcaatgcggg actgctgctc acgcgggtcg agtacctcaa
      301 gagtggcgcc gcaaagaact tcgcggtgac cgacgcggca atgaacgatc tgttgcgccc
      361 ggcgctctat gatgcctggc acgaggtcct gccagtgatg cggcgtagcg gcccggcgca
      421 gcgttacgat atcgtcggtc cggtctgtga atcggcggat tttctcgccc gggagcgcgc
      481 actcacggtc gctgcggggg acctcctcgc gatcatgtct gcgggtgcct acggcatgac
      541 gatgagctcg aattacaatt cccggccgcg tcccccggaa gtaatggtcg acgggaagac
      601 aactcacctc gttcgggatc gggaacgtgt cgagaacctc tttgcatcgg agcgtctttt
      661 gccgtaaatt taagcgtttg ccgtccaggc agcgagggac aacggcaggg gaccggggtc
      721 tgcggtagct gatggaatac gcggcttttt cacgtcgtta gcaaatccat gggtccttca
      781 ttgcgatcgc acgttgcggc agccacggcc aagcgcctgt tcgttctcgg cggcgtcggt
      841 ctgctcgtcg tcgcggtcgt gctgctcgcc tatttcggca ccgagattcg cgcgaacgag
      901 cccaaggcgt ccaagggacc cgccgcgatt cccgtctcgg ttgccccggt cctgcaggag
      961 gtcgtgccct tgcggctcct cgcgatcggc aacgtcgagg cctatgccac cgtgtcggtc
     1021 aaggcacggg tcgacggaca gatcgtggag gtcggcttca aggaaggcga cgaagtcccg
     1081 cgcagccgcg tgctcttcaa gattgacccg cgaccctacg aagcggcatt gcgccaggcc
     1141 gaagccaact tcctgcgcga cacggcgcag aagaaccagg cccgctcgca ggagaggcgc
     1201 taccaggaac tgctcgccaa gaatttcgtc tccaaggaag cctacgcgca gattcgcacc
     1261 aacgccgaca ccgccgaggc cgtggcccag gggagccgcg ccgcactcga caacgcggcg
     1321 ctcaatctgg agtactgcac gatccgctcg ccgatcgacg gctaccccgg aaagatccag
     1381 attcagatgg gaaacctcgt caaggcgaac gatacccttc ccctggtcgt gatcaaccag
     1441 gtccacccga tctacgtgaa cttctccgta ccggagcagc agctccgagc cgtgcgcggc
     1501 tacatgtcct cggggccgct cgcggtcgag gcgctggccc cgggctcgga tcaacccgca
     1561 gcctccggaa cgctggtttt cgtcgataac gcggtcgatc cgagcaccgg aaccatcaag
     1621 ttgcgggcgc agtttcaaaa cggcgagaac gcgctctggc ccggccagtt cgtcaacgtg
     1681 aggatcaagc tctacgacca gaaggacgcc ctcgtcgtcc catccaaatc ggtgcagacc
     1741 ggccccagtg gccaatacgt gttcgtggtc aagcccgaca tgaccactga acttcgcaaa
     1801 gtcacggtcg cgcgcaccga gggggacaac gccgtcatcg cgagcggtct ggaaaaaggc
     1861 gagcgggtgg tcacacaagg gcagctgcgc ctcggtgccg gagcgaaagt cgaggtcaag
     1921 tcgtaagttc tccaacatga atctatcgga actcttcgtc cgccgtccag tcatgaccgt
     1981 gctggtcatg gccgggattc tgattttcgg actggcgagc taccgcttgc tgcccgtttc
     2041 atcgctgccc aacgtcgatt tccccacgat acaggtgtcg gcccagcttc cgggtgcgag
     2101 cccggagacc atggccgcag cggtggcgac accgctcgag aagcagttct cgacgatcgc
     2161 cggcatcgac cagatgacct cgctctccgg ccagggcatc accagcgtca ccatccagtt
     2221 tacgctcgac cgcgacatcg acgctgccgc gcaggacgtc aattcggcga tcgctgccgc
     2281 gaccaagcag ctgccgccga ccatgtcgac acccccttcg ttccggaaag tgaatccggc
     2341 cgatgccccc atttttttcc tgacgctcac ctccgagacc ctgccgcttt ccaccgtcaa
     2401 cgagtacgcg gaaacctttc tcgcgcagcg catttcgaca atttccggcg tggcccaggt
     2461 ccaggtgcag gggcagcaga agtacgcggt gcgcgtgcag gtcgacccca acgcgctcgc
     2521 cgcgcgcggc gtcggcatca acgaggtcga gcaggcggtc gccaacgcca acgtcaacct
     2581 gcccaccggc acgctctacg gcaaggaccg catgttcgcc atacaggcga cgggccagct
     2641 ctacgaggct gctgccttcc gacccatcat cgtcacttac cgcaacggcg cgccgatccg
     2701 cttgagcgag ctcgggcgcg tcatcgacag cgtggagaac gacaagctcg ccgcgtggtt
     2761 caaggatcag cgcggcatca tacttgcagt ctatcggcag cccggcacca ataccatcga
     2821 ggtcgtggac gccgtcaaaa aactcttgcc gaccttccgc gttgaagtgc cggcaggggt
     2881 cagcatcaac gtgatgtacg accgatcgac cacgatccgc gaatcggtcg aggacgtgag
     2941 gttttcactg atcctggcgc tcgctctcgt ggtcatggtt atcttcatgt tcctccgcaa
     3001 cattccggcc actgttatcc cggcagttgc gctcccgatg tcgatcatcg gcacgttctc
     3061 gctgatgtac ctgttcggct acagtctgga caacatctcg ctgatggcgc tgactttgtg
     3121 tgtcggcttc gtggtggacg acgcgatcgt gatgctggag aacatctcac gccacatcga
     3181 gatggggaaa agcccgctcc aggcgaccct cgacgggtcg aaggaggttt ccttcaccat
     3241 cctgtcgatg acgatctcgc tcgccgccgt gttcattccg gtactcttca tgggcgggat
     3301 tctcggccgg ctgctccacg agttcgcagt caccatcatc gtcgcggtgc tgatctcggg
     3361 attcgtctcg ctcacgctga cgccgatgat gtgcagccgc atcctcaaac cgcgggccga
     3421 ggtcaagcac ggcaggctgt ttctggcgag cgagagagcg ttcgatgcgg tacggaacgc
     3481 gtacggacat accctgaact gggcgctccg tcgccaaaga ttcacgatgg gcgtgttcct
     3541 tgccatcgtc gttgccacgg gttggctcta cgtgaaaatg cccaaggggt tcctgccgag
     3601 tgaggatgcg ggacagctct tttgcttcat cgaagcg
//
LOCUS       DASPZD010000178         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_628955, whole genome shotgun sequence.
ACCESSION   DASPZD010000178 DASPZD010000000
VERSION     DASPZD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628955"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VEM33_03480"
     CDS             complement(<1..743)
                     /locus_tag="VEM33_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HYS50917.1"
                     /translation="MDPKQLLTLSFQAAIAAADPLKILPSHLPHPPSGRTLVIGAGKA
                     AASMAVAVEQHWPRDAALEGLVVTRYKHGLPTRRIRVIEAGHPVPDETGESAAKEILR
                     QVRSLAPNDLLLALISGGGSALLSLPVEAVGMDALKATTRELLRSGAPIQDINTVRKH
                     LSAIQGGRLAAASKAPVLALIISDVTGDDPTHIASGPCAPDPTTYRDALEVLHRYGVD
                     AAPEVRQHLERGARGEISETPKPGDGSFER"
     gene            complement(785..1630)
                     /locus_tag="VEM33_03485"
     CDS             complement(785..1630)
                     /locus_tag="VEM33_03485"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50918.1"
                     /translation="MNADKTNYDLLYNPRLLVKNYQEIFDRWGEQSERARASLGCYLD
                     VPYGPSEAEKMDVFRAQGKSRGLLMYIHGGYWRSLDKKRFSFVAPALAKAGITVAVPD
                     YALCPAVQVEDIVMQMVQACAWLYRNGENFGAPANRLHLCGHSAGGHLAAMMLCCRWP
                     DYSPDLPKKVVGAAMSISGLYDLAEIVKVPSVNCDVHLDERSALKVSPAFLPPATDAP
                     LYTAVGGEENEGFHMQNRLIGEKWGKVRETDLPCPGKNHFTVLDQVSDPESGLFKSML
                     EMMGA"
     gene            complement(1725..>2533)
                     /locus_tag="VEM33_03490"
     CDS             complement(1725..>2533)
                     /locus_tag="VEM33_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS50919.1"
                     /translation="NPGLRGRSSITHSLYAGLQLILPGEVAPLHRHSQSALRFILLGS
                     GAYTAVDGEKVTMSVGDFVITPSWTFHDHGNPSQEPMVWLDGLDVPLVELLDAQFMEK
                     GSQEAQHTGRTEGDNLAQFGNTMKPVEYRAKSRTSPLFWYPYERTREALETLGQAHDA
                     HPCWGHKLQYTNPVTGDWAMPTIGTFMQLLPKGFKGKDYRSTDSTVYAVVEGKGRCRF
                     AGETLVFGPRDVFVCPSWLRYRLEADEQAVLFSFSDRPVQQALDLWREAF"
BASE COUNT          439 a          785 c          814 g          495 t
ORIGIN      
        1 cgctcaaagc tgccgtcgcc cggcttgggc gtttccgaaa tctcgccgcg tgcgcctcgc
       61 tccaggtgtt gcctgacctc cggagccgcg tcgacaccgt agcgatggag aacttcgagc
      121 gcgtcgcggt acgtcgtcgg gtccggcgca cacggaccgg aggcgatgtg ggtggggtcg
      181 tcgcccgtaa catcggaaat gatcagcgca agcacgggcg ccttgcttgc ggcggcgagc
      241 cggccccctt gaatcgcgga aaggtgtttt cgcacggtgt tgatgtcctg aatgggagcg
      301 ccgctgcgaa gcagttccct cgtcgttgcc ttcagcgcgt ccatgccgac ggcctctaca
      361 ggaaggctga gcagcgccga gccgccgccg gatataaggg cgagcagaag atcgttcggc
      421 gcaaggctcc tcacttgtcg cagaatttct ttcgcggcgg actcgccggt ctcgtccgga
      481 acagggtgtc cggcttcgat gaccctgatg cggcgtgtcg gtagcccatg tttgtagcgc
      541 gtgacgacga ggccctcgag tgccgcatcc cggggccaat gctgctccac cgcgacggcc
      601 atcgaggcgg cggccttgcc cgcgccgatc acgagcgttc tgcccgacgg cggatggggg
      661 agatgactcg gaaggatctt gagcggatcg gcagcggcga tcgcggcctg aaagctcagt
      721 gtgagcaact gtttcggatc catgcgtcga ctctagccca actgggcggc ggtcccgggc
      781 tgctttacgc gcccatcatc tcgagcatcg acttgaacaa accgctttcg gggtcgctga
      841 cctggtcgag cacagtgaaa tgatttttcc ccgggcaggg taggtccgtc tcgcgcacct
      901 tgccccattt ctccccgatg agcctgttct gcatgtggaa tccctcgttt tcctccccgc
      961 ccaccgcggt gtagaggggg gcatctgtgg caggcgggag aaaagccggg cttactttca
     1021 gcgcggatct ctcgtcaagg tgcacgtcac aattcaccga gggtaccttg acgatctccg
     1081 ccaggtcgta caagccgctg atagacatcg cggcgccgac gaccttcttc ggcaggtccg
     1141 gcgagtagtc cggccagcgg cagcagagca tcatcgccgc cagatgcccc cctgcggaat
     1201 gcccgcagag gtggagtcgg ttggcgggcg cgccgaaatt ttcgccgttg cggtacagcc
     1261 acgcgcatgc ctggaccatc tgcatcacga tgtcttcgac ttgcaccgcg ggacaaagag
     1321 cgtaatcggg caccgcgacc gtgatcccgg ccttggcgag cgccggtgcg acgaaggaaa
     1381 agcgcttctt gtcgagcgaa cgccaatatc cgccgtggat gtacatcaaa agcccgcggc
     1441 tctttccctg ggcgcgaaag acgtccatct tttccgcttc gctcgggccg taaggcacgt
     1501 ccaaatagca gccgaggctg gcgcgggccc gctccgattg ttctccccag cgatcgaaga
     1561 tctcctgata gttcttgact aggagccgag gattgtagag gaggtcgtag tttgtcttgt
     1621 ccgcgttcat cttgttgttc cggagaggcg gtctcgccgc ggctcagggc gcacgaccgc
     1681 ctacgcgcgt ggaaacgcgg cgggcttcgc tgctttcagg tggattagaa cgcctcgcgc
     1741 caaagatcga gcgcctgttg caccgggcgg tccgagaaac tgaaaagcac cgcttgttcg
     1801 tcggcttcga gcctgtaacg cagccaggac ggacagacga atacgtccct gggcccgaag
     1861 acgagcgtct cgcctgcgaa acggcagcgg cccttgccct ccacgactgc atacaccgtg
     1921 gagtcggtcg agcgatagtc cttgcccttg aatcccttcg ggagaagctg catgaaggtg
     1981 ccgatggtcg gcatcgccca atcgcctgtc acgggattcg tatattgcaa cttgtggccc
     2041 cagcaggggt gtgcgtcgtg agcctgcccg agagtctcga gcgcttcgcg ggtgcgctcg
     2101 tacggatacc agaagagcgg cgaggtgcgg ctcttcgccc tgtactccac cggtttcatg
     2161 gtgttgccga attgcgcgag gttgtcgccc tcggtgcggc cggtatgctg agcttcctgc
     2221 gatccctttt ccatgaactg ggcatcgagc aattcgacga gcggaacatc gaggccgtcc
     2281 agccagacca tcggttcttg cgaagggttg ccgtggtcgt ggaaagtcca cgacggcgtg
     2341 atgacgaaat ctcccacgct catcgtcact ttctcaccgt ctaccgcggt gtaggcgccg
     2401 ctgccgagca aaatgaagcg cagcgcggat tgcgagtgcc gatgcaaggg cgcaacttcc
     2461 cccggcagga tcaactgcag tccggcgtaa aggctgtggg taatgctcga acggccccgc
     2521 agcccggggt ttt
//
LOCUS       DASPZD010000179         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_864227, whole genome shotgun sequence.
ACCESSION   DASPZD010000179 DASPZD010000000
VERSION     DASPZD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864227"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /gene="mrdA"
                     /locus_tag="VEM33_03495"
     CDS             complement(<1..1400)
                     /gene="mrdA"
                     /locus_tag="VEM33_03495"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYS50920.1"
                     /translation="MRLAEIRDTKRDLHYFQLRLGVAGVAVLAAFTILFGRFFYLQVI
                     QHQYYDTKAEDNRISIVPLQPNRGLILDRNGVVLARNYSAYTLELTFSKIADLEGTID
                     ELATIVDIQPKDRRRFQRLRDESKGRDTLPIRTRLTDEEVAKLAVNRYRFPGMDIKGR
                     LFRQYPLGEVASHVVGYISRIDDRDLERIEESDQSGNYKGADHIGKTGLEQRYERALH
                     GTTGFEQVETDAGGRIVPGRSGAQLAPRTAPVPGNNLALTLDIRLQEAAERAFGENRG
                     ALVAIEPTTGGVLAFVSKPGYDPNLFIDGIDPANWDALNNSPDKPLNNRALTGLYPPG
                     STFKPYMALAALELGTRTPSQTIFDPGYFVFGNNRFRDATAGGHGVVDMYRSIVVSCD
                     TYYYMLANDLGIDNIARFIGQFGFGSRTGIDIEGEFVGVLPSPEWKMKRFKTPGQQRW
                     FPGETISVGIGQGYNS"
     gene            complement(1407..1928)
                     /gene="mreD"
                     /locus_tag="VEM33_03500"
     CDS             complement(1407..1928)
                     /gene="mreD"
                     /locus_tag="VEM33_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYS50921.1"
                     /translation="MSFYERPQRILLPAKVGYVALTLVLALLFNLLPWRDVIGLPDMV
                     ALVLTFWCVHQPRKVGIGIAWFVGLLMDASNGALLGQHAFAYAFLAFAAVSLHRRILW
                     FSPWRQAAHVLVLLLASQMLMLAVRLVAGGVFPGLTYFAGTFVAAALWPTASFLLLLP
                     QRRAGSVDENRPI"
     gene            complement(1945..>2242)
                     /locus_tag="VEM33_03505"
     CDS             complement(1945..>2242)
                     /locus_tag="VEM33_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYS50922.1"
                     /translation="RGTLDLSYTAANADVLADDVLVTSGIDGTYPPGLPVAKVSRIEQ
                     DAAYSKITCVPTAGTDQNRQVLVLSRQMNLPAAPVETEPVAGKAPKPKRSRSRE"
BASE COUNT          391 a          695 c          744 g          412 t
ORIGIN      
        1 tatgagttat agccctgccc gatgcccacg ctgatggtct cccccgggaa ccatctttgc
       61 tgccccggcg tcttgaaccg cttcatcttc cactccggag atggcagcac gcccacgaac
      121 tcgccttcga tatcgattcc cgtgcggctc ccgaaaccga actggccgat gaagcgcgcg
      181 atattgtcga tgccgagatc gttggcgagc atgtagtagt aggtatcgca ggacacgacg
      241 atcgacctgt acatgtccac cacgccgtgg ccgcccgccg tggcgtcgcg gaatcgattg
      301 tttccgaata cgaagtagcc cgggtcgaag atcgtctgcg aaggcgtccg cgtgcccagc
      361 tcgagcgcgg cgagggccat gtagggcttg aacgtcgacc ccggcgggta aagtccggtg
      421 agcgcgcggt tgttgagcgg tttgtccggg gaattgttga gcgcgtccca gtttgccggg
      481 tcgatgccgt cgatgaatag attcgggtcg tagccgggct tggatacgaa ggcgagcact
      541 ccccctgtgg tgggctcgat cgcgacgagc gctccgcggt tttcgccgaa ggcgcgctca
      601 gcggcttcct gcaggcgaat atcgagggtg agagccaggt tgtttccggg cacgggagcc
      661 gtgcgcggcg cgagttgcgc gccgctgcgg ccgggcacga tgcgtcctcc cgcgtcggtc
      721 tccacctgtt cgaagccggt cgtgccgtga agcgcgcgct cgtagcgctg ctccagaccg
      781 gttttcccga tgtggtcggc gcctttgtaa ttgcccgact ggtcgctctc ctctatgcgc
      841 tcgaggtcgc ggtcgtcgat gcggctgatg taacccacca catgcgaggc gacttccccg
      901 agaggatact gccggaacaa cctccccttg atgtccatgc cggggaaacg gtaacggttg
      961 accgcgagct tggcgacttc ctcgtcggtg aggcgagttc gaatcggcaa ggtgtcgcgt
     1021 cctttgcttt cgtctctcag gcgctggaag cgccggcggt cctttggctg gatgtcgacg
     1081 atggtcgcaa gttcgtcgat cgtgccctcg agatccgcga tcttgctgaa cgtgagctcc
     1141 agcgtatagg cggaatagtt gcgggcgagc accacgccgt tgcggtcgag gatgagcccg
     1201 cggttaggct gaagcggcac gatcgaaatg cggttgtcct cggccttggt gtcgtagtac
     1261 tggtgctgga tcacctgcag gtagaaaaag cgtccgaaca ggatcgtgaa cgcggcgagc
     1321 acagcgactc cggcgactcc caggcgcagc tggaagtaat gcaggtcccg cttggtgtcc
     1381 cggatctcgg cgagacgcat gagtcttcag atcggccggt tctcgtccac gctcccggcc
     1441 ctgcgctgcg gcagcaggag cagaaagctc gcggtaggcc acaacgccgc ggcaacgaag
     1501 gtccccgcga aatacgtcaa cccggggaac actccgcccg ccacgagccg cacggcgagc
     1561 atcagcatct ggcttgcgag cagcaatacc agcacgtgcg cggcctgacg ccacggcgag
     1621 aaccacagaa tgcgccggtg caggctgact gcggcgaagg cgagaaaagc gtaggcgaag
     1681 gcatgctggc cgaggagcgc gccgttgctt gcgtccatca ggagccctac gaaccacgcg
     1741 atgccgatcc ccactttgcg aggctggtgc acgcaccaga aagtgagcac gagcgcaacc
     1801 atgtcgggca gcccgatgac gtcgcgccac ggcagcagat tgaacagcaa ggcaagcacg
     1861 agcgtgagcg ctacgtagcc caccttcgcg ggcaacagga tgcgctgcgg ccgctcgtag
     1921 aaggacacta gctctccctc gcgctcattc gcggcttctg gagcgcttcg gcttgggggc
     1981 cttgcccgcc acgggttccg tttccactgg ggcggccggg aggttcattt gccgcgacag
     2041 cacgagcacc tgccggttct ggtccgtgcc cgcggtgggc acgcatgtaa tcttggagta
     2101 ggcggcgtcc tgctcgatgc gcgacacctt ggccaccgga aggcccggcg gataagtgcc
     2161 gtcgatgccc gaggtcacga gcacgtcatc ggctagaacg tcggcgttcg cggccgtata
     2221 gctcaagtcg agcgtacctc tg
//
LOCUS       DASPZD010000180         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1750097, whole genome shotgun sequence.
ACCESSION   DASPZD010000180 DASPZD010000000
VERSION     DASPZD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750097"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VEM33_03510"
     CDS             <1..477
                     /locus_tag="VEM33_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50923.1"
                     /translation="HLAGELFKSMAKVNLVHVPYKGSAPSFQDLLGGQVSLTCDSLVQ
                     ALPYIRDKRLVALAVLGAARSPLLPEVPTVAESLPGYELTNWFGLVAPAAASRAIVGK
                     IHGDVVKVLQDAGVSEKLAAMGATAVGNTPEQFGAMMRADSEKWAKLIREAGIRAE"
     gene            484..1251
                     /locus_tag="VEM33_03515"
     CDS             484..1251
                     /locus_tag="VEM33_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYS50924.1"
                     /translation="MEAIKLDDYEIDVLIQGFPGRMVCHGGLGWSTIALIRGRGHVAL
                     VDVGSFSMRKGLIAQLAKRGLEPADVTDVILTHSHYDHSLNWILFRQARIIIGARELA
                     WSMSEPWGETPVPELYIRELESWPSLQPVAAEQECIPGLKAYAAPGHTPGHLVFVLEG
                     KERDAIFTGDAAKNRAELLSRKGEATYDAALSAASIERIWQLWRKRPGSVLVPGHDLP
                     MTQRDGHIEYIGTRKAALKAWFGEDIETSTLIELGVR"
     gene            1284..2264
                     /locus_tag="VEM33_03520"
     CDS             1284..2264
                     /locus_tag="VEM33_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50925.1"
                     /translation="MSKTPKSTTRVLLIALFAAALHATHAAAQVYPSKPVRMIIPFPP
                     GGSNDVVGRMIAFQLSERLGKQVVVDNQGGAGGIIGTEAVARAPADGHTLLLISVAHA
                     FGASMYKLPYDPISAFAPVALLGTGPVVLAVTTKLPVDSLKDLLALAKEKPGQLNYAS
                     AGVGSFQHLASSLFKLQSGLDIVHIPFKGGGPAMMDVIAGNTQIAIGSLIQTLPHIRS
                     GRLKPLGVGSTKRVAALPDLPTISEAGVPGYEATNWWGIVAPAGTPRPVIERLHKELS
                     VILASAETKKRFETEGGEAAQMSPEEFGRFIASETVKWAKVVKEAGIKPE"
     gene            2222..>2474
                     /locus_tag="VEM33_03525"
     CDS             2222..>2474
                     /locus_tag="VEM33_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50926.1"
                     /translation="MGEGREGSRHQAGVGIITDLARLLAGCCAVIACSLPPAAAQGPA
                     VGKIPSFAELEAAGAVIGEVRIDTRDIFDLADEKENGILY"
BASE COUNT          452 a          825 c          821 g          376 t
ORIGIN      
        1 cacctcgcgg gcgagctctt caagagcatg gcgaaggtaa acctcgttca cgtcccgtac
       61 aagggcagcg cgccgagctt ccaggatctg ctcggagggc aggtgtcgct cacctgcgac
      121 agcctggtgc aggcgttgcc ctacatacgc gacaagcggc tcgtcgcgct cgcggtgctc
      181 ggagccgcgc gctcgccgct gctccccgag gtgccgaccg tcgccgaatc gctccccggt
      241 tacgagctca ccaactggtt cggcctggta gcgcccgcgg cagcgtcccg cgcgatcgtc
      301 ggtaagatcc acggcgacgt ggtcaaggtg ctgcaggacg ccggggtgtc ggagaaactt
      361 gccgcgatgg gcgcgaccgc ggtcggcaac acccccgagc agttcggcgc gatgatgcgc
      421 gcggattccg aaaaatgggc caagctgatc cgggaagccg gtatccgggc ggagtgaaaa
      481 aaaatggaag caatcaagct ggacgactac gagatcgacg tgctcatcca gggctttccc
      541 ggcaggatgg tctgccacgg gggcctgggc tggagcacga tcgccctgat ccgcggccgc
      601 gggcacgtcg ccctggtgga cgtcggcagc ttcagcatga ggaagggcct gatcgcgcag
      661 ctcgcgaagc gcggcctgga gcctgccgac gtgaccgacg tcatcctcac gcattcgcat
      721 tacgatcatt ccctcaactg gatcctgttc cgccaggcgc ggatcatcat cggcgcaaga
      781 gagctcgcct ggtcgatgtc cgagccctgg ggcgagacgc ccgtgcccga gctgtacata
      841 cgcgagctgg aaagctggcc ctcgctgcaa cccgtcgcag ccgagcagga atgcatcccg
      901 gggctcaagg cgtacgccgc gcccggacac accccggggc atctcgtctt cgtgctcgag
      961 ggaaaggagc gcgacgcgat cttcaccggc gacgccgcga agaaccgcgc ggagctgctc
     1021 tcgcgcaagg gcgaagccac ctacgacgcc gcgctcagcg cggcgtcgat cgagaggatc
     1081 tggcagctgt ggcgaaaacg gccgggaagc gtcctcgttc ccggccacga cctgccgatg
     1141 acgcaacggg acggacacat cgaatacatc ggtacacgca aagccgcgct caaggcgtgg
     1201 ttcggcgagg acatcgagac atccacgctc atcgagctgg gcgtgcgctg aaggggcgga
     1261 agaagcttgc catggaggag agaatgtcga agacgccgaa atctacgacc cgtgtcctgc
     1321 tgatcgcgct ctttgcggcc gcgctgcatg cgacgcacgc agcggcgcaa gtctacccga
     1381 gcaagccggt gcggatgatc atcccgtttc cgcccggcgg cagcaacgac gtggtcgggc
     1441 gcatgatcgc attccagctt tccgagcggc tcggcaagca agtggtcgtg gacaaccagg
     1501 gcggcgcggg cggcatcatc ggcacggagg ccgttgcgag agcgcctgcc gacggacaca
     1561 cgctgctcct gatctcggtc gcccacgcgt tcggcgcctc gatgtacaag ctgccctacg
     1621 atccgatcag cgccttcgct ccggtcgccc tgctcggcac gggacccgtg gtgctcgccg
     1681 tcaccacgaa gctgccggtc gattccctga aggatctcct cgccctcgca aaggaaaaac
     1741 ccgggcagct gaattacgcc tccgccggcg tcggcagctt ccagcatctg gcgagctcgc
     1801 tgttcaagct gcaatcgggc ctggacatcg tgcacattcc gttcaagggc gggggccccg
     1861 cgatgatgga cgtgatcgcg ggcaacaccc agatcgccat cggctcgctc atccagacgc
     1921 tgccccatat ccgttcggga cgcctgaagc cgctgggcgt gggaagcacc aagcgcgtcg
     1981 ccgccctgcc cgatctgccg acgatctcgg aggcgggggt tcccggttac gaagccacca
     2041 actggtgggg aatcgtcgcg cccgccggca cgccgcgccc ggtcatcgag cggctgcaca
     2101 aggagctctc ggtgatcctc gcgtcggccg aaacgaagaa gcgcttcgag accgaaggcg
     2161 gggaagccgc gcagatgagc cctgaggagt tcggccgctt catcgcctcc gaaactgtga
     2221 aatgggcgaa ggtcgtgaag gaagccggca tcaagccgga gtaggaatca ttaccgacct
     2281 cgcccggttg ctcgccggct gctgcgcggt gatcgcttgc tcgctcccgc cggcggcggc
     2341 ccaggggccc gccgtaggga aaatcccgtc cttcgccgag ctcgaggctg ccggagcggt
     2401 catcggcgag gtgcggatcg acacccgaga catcttcgac ctcgccgacg agaaggaaaa
     2461 cggcatcctc tacc
//
LOCUS       DASPZD010000181         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_803895, whole genome shotgun sequence.
ACCESSION   DASPZD010000181 DASPZD010000000
VERSION     DASPZD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803895"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VEM33_03530"
     CDS             complement(<1..330)
                     /locus_tag="VEM33_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50927.1"
                     /translation="MSAQPQDLKVFISGRDSTCDECGQELGRSAWICLLGERGALCLS
                     CADLDQLVFLPAGDVALTRRARKHSTLSAVVLKWSHARKRYKRQGLLVDEAGLATAES
                     ECLADADA"
     gene            complement(472..768)
                     /locus_tag="VEM33_03535"
     CDS             complement(472..768)
                     /locus_tag="VEM33_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYS50928.1"
                     /translation="MARVVLEAEVLDDFERFLVHLLQHDVQNAPERIQEIVDALQILE
                     TSPHLGRPTADGFRELVIGTGARGYIALYVYDPERDAVFVVAVRSQKEAGYRRG"
     gene            complement(770..1054)
                     /locus_tag="VEM33_03540"
     CDS             complement(770..1054)
                     /locus_tag="VEM33_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYS50929.1"
                     /translation="MSTTTIRIDEDLKARVAVAAERAGKTAHGFIVDAIAQATEQSEL
                     ESDFHQVAAKRLARILATGEGVPLGEVRDYLFARVRGETPRRPKPRKLRT"
     gene            complement(1409..1702)
                     /locus_tag="VEM33_03545"
     CDS             complement(1409..1702)
                     /locus_tag="VEM33_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYS50930.1"
                     /translation="MKLAIHRLAVAEIDHEVDYYESCSAGLGTELEDEIDAAIETILR
                     FPQAAPQWRDRRDRRVAVLDRFPFTLVYQIGAEEIVVLALAHTSRRPGYWSRR"
     gene            complement(1699..1938)
                     /locus_tag="VEM33_03550"
     CDS             complement(1699..1938)
                     /locus_tag="VEM33_03550"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HYS50931.1"
                     /translation="MGATDILADILRLPAEERARLALELIRSLDGESDADAMQAWDAE
                     IRRRGDEVDGHSAESMTLDEYRAHIRRRRAARAIR"
     gene            complement(2034..2300)
                     /locus_tag="VEM33_03555"
     CDS             complement(2034..2300)
                     /locus_tag="VEM33_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50932.1"
                     /translation="MLDVFRLAWFAVRTFFGVIALVLALYAALWLFVKILSAAPSNDE
                     PGAALRSVESYNSQAASRGRATAAVPHDRSGTCPESRSARCEQP"
BASE COUNT          456 a          855 c          842 g          469 t
ORIGIN      
        1 cgcatcggca tcggccaggc attcggattc ggccgtcgcg agtcctgcct cgtcgaccag
       61 cagaccctgt cgcttgtatc gcttgcgcgc gtggctccac ttcaagacga ccgcggacag
      121 cgtagagtgc ttgcgcgccc ggcgcgtcag tgcgacgtcg cccgccggca ggaagacgag
      181 ctgatcgagg tcagcgcagg acaggcaaag agcgccgcgt tcacccagga ggcagatcca
      241 ggcgctgcgg cccagttcct ggccgcattc gtcgcacgtg gaatcacgac ccgaaatgaa
      301 taccttgaga tcttgtggtt gtgcgctcat cgaaagtccg agttctccgt caagcggcgc
      361 gcccgaagga aaagcgtcat gactcgaatt tctcctcagg acatggctcg tgcggtcctt
      421 ggccggccgt gatacgcggc cgaccggact ggagctggag aaggttctcg gctaaccgcg
      481 cctgtaccct gcctctttct ggctgcgcac cgccacgacg aaaacggcgt cgcgctccgg
      541 gtcgtacacg tacagcgcga tgtaaccacg cgccccggtt ccgataacga gctcgcgaaa
      601 gccgtcggct gttggccgcc caaggtgcgg actggtctcg aggatctgca gggcgtcgac
      661 gatctcctgg atgcgctcgg gcgcgttctg gacgtcgtgc tgcagcaggt gcaccagaaa
      721 ccgctcgaaa tcgtcgagaa cttccgcttc gaggacgact cttgccaaat caggtcctca
      781 gcttgcgtgg ctttggccgc cttggggtct cgccgcgcac tcgagcaaac aggtagtcgc
      841 ggacttcccc cagcggcacg ccttcgccgg tggccaggat cctggccagg cgcttggcgg
      901 cgacttggtg gaagtcgctc tccagctcgg attgctcggt cgcctgcgcg atcgcgtcga
      961 cgatgaagcc atgtgcggtc ttgccggcgc gctcggcggc gacggccacg cgggctttca
     1021 gatcctcgtc gatacggatg gtcgtggtgg acacggggcg cctttcaacg gcacgggact
     1081 gggatagtgt agcacatttg tgcgacatcc atcacggtca tcgaatcccg gcgtgcccgg
     1141 tcaatcgttg gtgagaagcc cgactgcgtc aggcggacgt gctttcaagc ggctctggac
     1201 gggcggaacc tgccgccgac caggcggccg atcgttcgcg tgccgtcggc tggttctcgg
     1261 atgatcttgg acggatcgat accgggccca gcagttcatc gccgacatag agacactgtt
     1321 tccccgtcca ttcgacggag gaatcgacaa tcgcgtaggc gataacacga gaaaaatcga
     1381 ttacgggcgg cggagcggcg ctcatacttc accgacgcga ccagtagccc ggccggcggc
     1441 ttgtgtgcgc aagcgcaagg actacgatct cttccgcccc gatttggtag acgagcgtaa
     1501 acggaaagcg gtccagaacg gccacccgac ggtcgcgccg atctctccac tgaggtgccg
     1561 cctgcggaaa ccgaaggatc gtttcgatag cggcatcgat ctcgtcttcc aactctgtgc
     1621 cgaggccggc actgcacgat tcgtaatagt cgacctcgtg atcgatctca gcgaccgcga
     1681 gtcgatggat tgcgagcttc aacgaatcgc gcgagccgcg cggcgtcggc ggatgtgggc
     1741 gcggtactcg tcgagtgtca tcgactcggc tgagtggccg tcgacctcgt cgccacgacg
     1801 ccggatctcg gcgtcccacg cttgcatggc atcagcgtcg gattcgccgt cgagactgcg
     1861 gatcagctcg agcgcgaggc gcgcgcgctc ctccgctggg aggcggagaa tatctgcaag
     1921 gatgtcagtc gctcccacgg caagtatgtt acccgattgc cgagcgcaga ccgcgctgtg
     1981 gcgggctcac gcgcgcctcg tagcgccgtg gggaccgcgc cccgcaggcc ttctcacggc
     2041 tgctcgcagc gggcgctgcg cgattcgggg caagtgcccg agcggtcgtg gggaacggcc
     2101 gccgttgccc gcccgcggct ggcggcctgc gagttgtagg actccacgct tcgcaatgcc
     2161 gcacccggct cgtcgttgct aggcgccgcc gagaggatct tcacgaacag ccatagcgcc
     2221 gcgtacagcg caagaacgag cgcgatcacc ccgaagaaag tgcgcaccgc aaaccacgcg
     2281 aggcgaaata cgtccaacat ggctttcgtc ctaggcaaac tccccgccgt gatacacccg
     2341 gttaacgacg gttcaatcag tgcgaagtcg gaagcgctgc caatccccgc accctgcttc
     2401 actttcctgc atgcacctgc accgtgagcc gtttcttgtc cgaactcacc ggcatttatc
     2461 cgataggacc aaccgccggc gccttcaagc ggtcttctgg gcctcccgcg ctggcaagcg
     2521 gatgacggag gcggcgtcgg gcggctcggt cttggccgcg ctttccttgc cttcgcagaa
     2581 atcggcccac gcccgaagca gggccgcgcg ctcggcgtgg aa
//
LOCUS       DASPZD010000182         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_630592, whole genome shotgun sequence.
ACCESSION   DASPZD010000182 DASPZD010000000
VERSION     DASPZD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630592"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VEM33_03560"
     CDS             complement(<1..532)
                     /locus_tag="VEM33_03560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS50933.1"
                     /translation="MPKRHRRVVTGHDAKGRSVVLMDGESPYSFSLEKAGGLQLTELW
                     ETRSSPADNSGAKDAADHERRIEPVDGGTVFRIIEYPPDRVRLATIDPESFYPAMGAE
                     AAGQAERRHPGTHRTRTLDYCVVLSGEIWAMLDKGEVLLRAGDCLVQRGTRHAWSNRS
                     EEPCVIAFVLVAAKPLP"
     gene            complement(567..1574)
                     /locus_tag="VEM33_03565"
     CDS             complement(567..1574)
                     /locus_tag="VEM33_03565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS50934.1"
                     /translation="MKHDLPLVVDFHVHMLEEGVFKASTNKTVFTGFGANPMPAPRPG
                     AAQLMHRMFNPKLEIEDMDARGIDVSVVTSSTVLQGTSWADPQTDLELCMRCNDKAAE
                     WQAQYPHRFAGSFVLPLQDVKRSLSELERAVTKLKLKVANIGAQYGGAYMGDPVFHPF
                     WEAIQAHDVAVWIHPEGARDPWFQRYALWNSAGQSIEEAKCLTSLVYEGVMHRHPKLK
                     VVIAHGGGYFPHYLGRMDRNTLNRPDTVKNIGGRNPSEFLRSFHYDTCVYDPKVLQVL
                     LERVGADHLVLGSDYPVGERDPVGWLRDDCGLSGADLAKVAGGNAARLLGLLPRARTA
                     A"
     gene            complement(1585..2553)
                     /locus_tag="VEM33_03570"
     CDS             complement(1585..2553)
                     /locus_tag="VEM33_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50935.1"
                     /translation="MTRLLQTLLIAALLGGFADAQVQTWPTRPIKVIVSFPAGSAPDI
                     VCRFVTERLARAVGQQLIVDNRPGSGNILAGQAAARSAPDGYNFFCATAATLVSNPHT
                     FKSLPYDPARDFVPVAMIAKGPFFVLVHPSVPVKTLAELVAYDKANPGKLAWATDGLR
                     NFTGMMAAWLNKIAGTGILTVPYTTMPLGIQDTLAGRTQLVILAVPAAAPRIRSGELR
                     PLAASFAKRVPGYEDVPAISETFPGFELVGWFLVVAPTGTPKEIVLRMNRELDGILKD
                     PETVQGLRGLGFFTDGAETPEAVAEAIRADTAKWGRIVREIGIQPE"
     gene            complement(2560..>2788)
                     /locus_tag="VEM33_03575"
     CDS             complement(2560..>2788)
                     /locus_tag="VEM33_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS50936.1"
                     /translation="GWFALVAPAGTPAEAVQKLNRDVNKVLADAEVSQRLRELGAIYD
                     GPGTPQDLAEFLKAERARWAKLVKDIGLQPE"
BASE COUNT          431 a          936 c          944 g          477 t
ORIGIN      
        1 acggcagcgg cttcgcggcg accagcacga atgcgatcac gcagggctcc tcgctgcggt
       61 tgctccaggc gtggcgggtg ccgcgctgca cgaggcagtc gcctgcgcgc agcagcactt
      121 cgcctttgtc cagcatcgcc cagatttcgc ccgagaggac gacgcagtag tcgagcgtgc
      181 gcgtcctgtg cgttccggga tgacggcgct ccgcctgtcc ggcggcctca gcacccattg
      241 cggggtagaa ggattcggga tcgatcgtcg caagccgcac gcggtcgggc ggatactcga
      301 tgatgcggaa cacggtgccg ccgtccacgg gctcgatgcg ccgctcgtga tcggccgcgt
      361 ctttcgcgcc cgaattgtcc gctggcgagg agcgcgtctc ccacagctcg gtcagctgca
      421 gtccaccggc cttctcgaga gaaaaactat aaggcgattc gccgtccatg agcacgacgg
      481 agcgcccttt ggcgtcgtgc ccggtgacca ctctgcggtg ccgcttgggc attttccctc
      541 cggggcggag gctcgcccgg ccgtgttcag gccgcggtgc gcgcgcgcgg aagcaggccc
      601 aaaaggcgcg cggcgttgcc gccggcgacc ttcgcgaggt ccgcgccgct caggccgcaa
      661 tcgtcccgca gccagccgac cgggtcgcgc tcgccgaccg ggtaatcgct gccgagcacg
      721 aggtgatcgg cgccgacgcg ctcgagcagc acctgcagca ccttcggatc gtacacgcag
      781 gtgtcgtagt ggaaggagcg caggaactcg ctcggattcc tgccgccgat gtttttcact
      841 gtgtcgggcc ggttcagcgt attgcggtcc atgcggccga gataatgcgg gaagtaaccg
      901 ccgccgtgcg cgatcacgac cttcagcttt ggatgcctgt gcatcacgcc ttcgtagacg
      961 agcgaggtga ggcactttgc ctcctcgatc gactggcccg cggagttcca cagcgcatag
     1021 cgctggaacc agggatcgcg cgcgccttcg ggatggatcc acacggcaac atcgtgcgcc
     1081 tggatcgctt cccagaatgg atggaacacc gggtcgccca tgtatgcgcc gccgtattga
     1141 gccccgatgt tggcgacttt gagtttcagc ttcgtcaccg cgcgctccag ctcgctaagc
     1201 gagcgcttca cgtcctgcag cggcagcacg aagctgccgg cgaagcggtg cgggtattgc
     1261 gcctgccatt ccgctgcctt gtcgttgcag cgcatgcaca gctctaggtc ggtctgcgga
     1321 tcggcccagg aggttccctg cagcaccgtg ctcgaggtga cgacgctcac gtcgatgccg
     1381 cgcgcgtcca tgtcctcgat ctccagtttc ggattgaaca tgcggtgcat cagctgcgcc
     1441 gcccccggcc gcggcgcggg catcggattc gcgccgaagc cggtgaacac cgtcttgttg
     1501 gtcgacgcct tgaacacgcc ttcctccagc atgtgcacgt ggaaatcgac gacgagggga
     1561 agatcgtgct tcacgattgg agtcctattc cggctggatg ccgatttccc tcacgatcct
     1621 gccccatttc gcagtatcgg cgcggatcgc ctccgccact gcctcgggcg tctcggcccc
     1681 gtcggtgaag aagcccaggc cgcgcaggcc ttgcaccgtt tccggatcct ttaggatccc
     1741 gtccagctcg cggttcatgc gcagcacgat ctctttcggc gtgccagtcg gcgcgacgac
     1801 gagaaaccag ccgaccagct cgaaccccgg aaaagtttcg ctgatggccg gcacgtcctc
     1861 gtagccggga acgcgcttcg cgaagctcgc ggcgagcggg cgcagctcgc cgctcctgat
     1921 gcgcggcgcg gccgcgggaa cggcgaggat gacgagctgg gtgcggcccg cgagcgtgtc
     1981 ctggatcccg agcggcatgg tcgtgtaggg aacggtgagg atcccggtcc ccgcgatctt
     2041 gttgagccac gccgccatca tgccggtgaa gttcctcagg ccgtccgtgg cccaggcgag
     2101 cttgccgggg ttcgccttgt cgtaggcgac gagttccgcg agcgtcttca ccggcacgct
     2161 cggatgcacg aggacgaaga acggaccctt ggcgatcatc gccaccggca cgaaatcgcg
     2221 cgccggatcg tagggcagcg acttgaaggt gtgcggattg gaaacgaggg tcgccgccgt
     2281 ggcgcagaag aagttgtagc cgtcgggggc ggagcgcgcc gccgcctgcc cggcgaggat
     2341 gttccccgag cccgggcggt tgtcgacgat gagctgctgg ccgaccgcgc gcgcgaggcg
     2401 ctcggtgacg aagcggcaga cgatgtcggg cgccgatccc gccgggaacg acacgatcac
     2461 cttgatcggc cgcgtcggcc aggtttggac ctgcgcatcg gcgaaaccgc cgagcagcgc
     2521 cgcaatcaac agcgtctgga gcaggcgggt catggcgcgc tactccggct gcaggccgat
     2581 gtccttcacg agctttgccc agcgcgcgcg ctcggccttc aagaattccg cgagatcctg
     2641 cggcgtaccc gggccgtcgt aaatggcgcc gagctcgcgc aggcgctgcg acacctcggc
     2701 gtcggccagc accttgttga cgtcgcggtt cagcttctgc acggcctccg cgggcgtgcc
     2761 cgccggcgcg accagggcga accagccg
//
LOCUS       DASPZD010000183         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_140735, whole genome shotgun sequence.
ACCESSION   DASPZD010000183 DASPZD010000000
VERSION     DASPZD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140735"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VEM33_03580"
     CDS             <1..363
                     /locus_tag="VEM33_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYS50937.1"
                     /translation="WVNHYCVEGWTAVETWTGVPFTELARLVRADPAARFVDFQSFDD
                     DYHESWDIESAMHKQTVVAYGLDGKMLEPAHGAPSRVYSPVKLGYKNTKYLTKIVFTP
                     EMNGGYWSDQGYEWYGGV"
     gene            complement(572..952)
                     /locus_tag="VEM33_03585"
     CDS             complement(572..952)
                     /locus_tag="VEM33_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50938.1"
                     /translation="MKVEQFPLLLGVLVALVGLTILLDAWQAGGVAPLRERRRRTRAV
                     PNKGGQTLVAFGTMCMAAALLGRDTWRYGTISVLAGAALLVIGAIMNRAYLKEVLLFR
                     GAARRGEGEKNSRLNQTPPRTRIR"
     gene            1034..1582
                     /gene="hslV"
                     /locus_tag="VEM33_03590"
     CDS             1034..1582
                     /gene="hslV"
                     /locus_tag="VEM33_03590"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HYS50939.1"
                     /translation="MKFDRERVRSTTILGVRRNGQVALGGDGQVTVGQTVMKSNAQKV
                     RTLHGGKLLAGFAGAAADAFTLFEKFEEKLERYPGNLQRAAVELAKDWRSDRVLRRLE
                     ALLAVTDKEHGFIISGTGDIIEPDDGILAIGSGGSYALAAARALLESTDMAPREIVRR
                     GLEIAAEICVYTNANITVLEPS"
     gene            1582..>2308
                     /locus_tag="VEM33_03595"
     CDS             1582..>2308
                     /locus_tag="VEM33_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS50940.1"
                     /translation="MASPRTQQALERLAGLTPKQIVEELDRYIVGQADAKKAVAIALR
                     NRWRRQRAPEKIREEISPNNIILVGPTGVGKTEIARRLARLAGAPFVKVEASKFTEVG
                     YVGRDVESMVRDLVESAINMVRGERETEVQEIAKQKVEERLLDLLLPVPPDIKQSTHT
                     SAAAGGGAHLFVVSPGGSVSSETDASEVRYKRTREKLKALLNEGQLDEREIEIEVTQA
                     PVMFDMMVPQGAPEGMDNLSEMRQ"
BASE COUNT          460 a          700 c          752 g          396 t
ORIGIN      
        1 tgggtcaacc actattgcgt cgaaggctgg accgcggtcg agacctggac cggagttccg
       61 ttcaccgagc tggcgcgact cgttcgcgcc gatcccgccg cgcgattcgt cgactttcag
      121 tcgttcgacg acgactatca cgaaagctgg gacatcgaga gcgccatgca caagcagact
      181 gtcgtcgcct acggtctcga tggaaaaatg ctcgagcccg cgcacggcgc tccctctcgc
      241 gtctactctc cggtcaagct cggctacaag aacaccaagt acctgacgaa gatcgtgttc
      301 acgccggaga tgaacggagg gtactggtcg gatcagggtt atgaatggta cgggggcgtg
      361 taatcaaggc gggccggagc gggtcgccaa ctagctgcca tccacaaaac taaagcgggg
      421 agctgtaagc ttagtaagcc aagcagcttc gagcagctca tagcgggaga gctccgggcg
      481 gctcggggcc gcaagcttac caagcttaaa gctccccgct ttagttttgt ggtggctatt
      541 gcgcggctgg atctagggcc cgccttgatc tttacctgat ccgagttctg ggcggcgttt
      601 gattcaaccg cgagttcttc tcgccctctc cccgcctcgc cgccccgcga aagagaagaa
      661 cctctttcag gtacgcgcga ttcataatcg cgccaatcac gagcaacgcg gccccggcga
      721 ggaccgagat cgtcccgtac cgccacgtgt cgcggccgag gagggcggca gccatgcaca
      781 tcgtaccgaa ggcaaccagc gtctggccgc ctttgttcgg caccgcccga gtgcggcgcc
      841 gacgctcccg aagaggggcc actccgcctg cctgccaggc atcgagcagg atcgtcaggc
      901 caacaagagc caccaggacg ccgagcagga gtgggaattg ttcaactttc atgacacata
      961 tgaatgcaag aagggggcac aagttgcggg cgtcctggcg ctcctaacac ggaagaggcg
     1021 ttaatttgaa gcgatgaaat tcgatcgcga gcgcgttcgt tcgaccacga tactgggcgt
     1081 gcgcaggaac gggcaggttg cgttgggcgg tgacggccag gtcaccgttg gtcagacagt
     1141 gatgaagtcg aacgcgcaaa aagtgcgtac gttgcacggc ggcaagctgc tcgcgggctt
     1201 cgccggtgct gctgccgacg ccttcactct cttcgagaag ttcgaggaga aactggagcg
     1261 ctaccccgga aatcttcagc gcgccgcggt cgagctggcc aaggattggc ggagcgatcg
     1321 ggtgctccgc cggctcgagg ctctcctcgc cgtcaccgac aaggagcatg gtttcatcat
     1381 ctccggcact ggcgacatca tcgagcccga cgacggcatt ctcgcgattg gctcgggcgg
     1441 atcgtatgct ctcgctgccg ctcgcgctct tctcgagagc accgacatgg cgccgcgcga
     1501 gatcgtgcgc cgcggtctgg agatcgccgc cgagatctgc gtttacacca atgccaatat
     1561 cacggtgctg gagccaagct gatggcgtcg ccacgaacgc aacaggcgct ggagcgactg
     1621 gcggggctga ccccgaagca gatcgtcgag gagctcgatc gatacatcgt cggccaggcc
     1681 gatgccaaga aggcggtcgc catcgcgctc cgcaatcgct ggcgccgcca gcgtgcgccg
     1741 gagaagattc gcgaggagat ctcgcccaac aacatcatcc tcgtcggtcc gactggagtg
     1801 ggtaagacgg agatcgcgcg caggctggcg cgtctagccg gggcgccctt cgtgaaagtc
     1861 gaggcgtcga aattcaccga ggtgggctac gtcggtcgcg acgtcgaatc gatggtgcgc
     1921 gatctggtgg agagcgcgat caacatggtg cgtggcgagc gcgagacaga agtccaggag
     1981 atcgcgaagc agaaagtgga agagcggctg ctcgaccttc tgctgccggt cccgcccgac
     2041 atcaagcagt cgacgcacac atccgcagcc gccggcggtg gtgcgcatct cttcgtcgtc
     2101 tcgcccggcg gctctgtctc cagcgagacc gacgcgtcgg aggtgcgcta caagagaacg
     2161 cgcgagaagc tcaaggcgct cctcaacgag ggtcagctcg atgagcgcga gatcgagatc
     2221 gaggtcaccc aggcgccggt gatgttcgac atgatggtgc cgcagggcgc gccggaggga
     2281 atggacaatc tctccgagat gcgccagg
//
LOCUS       DASPZD010000184         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_538418, whole genome shotgun sequence.
ACCESSION   DASPZD010000184 DASPZD010000000
VERSION     DASPZD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538418"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /gene="pgsA"
                     /locus_tag="VEM33_03600"
     CDS             complement(<1..342)
                     /gene="pgsA"
                     /locus_tag="VEM33_03600"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HYS50941.1"
                     /translation="MPLNVPILLTWLRILTIPLVIGVFYVPEVWLSSWRQNLIATSLF
                     IAAAITDWLDGYLARRLNQTSAFGAFLDPVADKLMIAAALIVLVWLQRVDAIIAVVII
                     GREIAVSAMREW"
     gene            complement(332..>2057)
                     /gene="uvrC"
                     /locus_tag="VEM33_03605"
     CDS             complement(332..>2057)
                     /gene="uvrC"
                     /locus_tag="VEM33_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HYS50942.1"
                     /translation="EALYVGKAGDLKKRVSSYFQKTQHGPRIAMMLTQVAAVEITVTR
                     SEAEALILENNLIKSLAPRYNILFRDDKSYPYLMVSGGEFPRLGFHRGAMDKAHHYFG
                     PFPHAGAVRESIQLLQRVFRLRTCEDSVFQNRSRPCLLYQIRRCTAPCVGHISAAAYA
                     EDVKSAQLYLQGRSDTALKRLEARMQQASDARRYEEAAIYRDQIQSLAKVSQRQYADT
                     GADVDVDIIAVAMEHGIACVNLVMVRGGRQLGDRSLFPQNAEERDAKEVLSAFIAQHY
                     LERPIPPALVVGEDLDVQELEATLSEHAKRSVRVHTRPTAERRAWLEMARQNALQALK
                     SKLTEHSTQETRLAALREALGLPDTVQRIECFDVSHTMGEATVAACVVYDRMDMSRGE
                     YRRYNIEGVQPGDDYGALAQALSRRYGRIAGGEGVAPDLIFIDGGKGQVSAAREALAE
                     VGLSDIALVGIAKGPERKPGLEELWVGERVVTLAPDHPGLHLIQRIRDEAHRFAIAGH
                     RARRSKRRTTSTLEGIAGIGAKRRKQLLTRFGGLKGVMGASVEDLTQVEGISRKLAEK
                     IYRELHAA"
BASE COUNT          327 a          686 c          671 g          373 t
ORIGIN      
        1 ccattcgcgc atcgccgaca cggcgatctc ccggccgatg atgaccacgg cgatgatcgc
       61 gtccacgcgc tgaagccaca cgagcacgat cagcgccgcg gcgatcatca gcttgtcggc
      121 gaccgggtcg aggaacgcgc cgaacgccga tgtctggttc aagcgcctcg cgaggtagcc
      181 atcgagccag tccgtgattg cggccgcgat gaacaacgag gtcgcaatca ggttttggcg
      241 ccaactcgag agccacacct ccgggacata gaagaccccg atcaccaacg ggatcgtcag
      301 gatgcggagc caggtgagaa ggatcgggac gttaagcggc atggagttcc cggtagatct
      361 tttccgcgag ctttcgactg atcccctcga cctgcgtcag gtcctcgacg ctcgccccca
      421 tgacgccctt cagcccgccg aaacgcgtga gcagctgctt gcggcgtttg gcgccgatcc
      481 cggctatgcc ctccagcgtc gaggtcgtgc gccgcttgga gcgtcgcgcc cggtgcccgg
      541 cgatggcgaa acggtgcgcc tcgtcgcgaa tccgctggat caggtgcagt ccggggtggt
      601 ccggggcaag cgtcaccacg cgctcgccca cccacagctc ctcgaggccg ggctttcttt
      661 ccgggccctt tgcgatgccc acgagcgcaa tgtcggagag gccgacttcc gcgagcgctt
      721 ctctcgccgc gctcacctgt cccttgccgc catcgatgaa aatcaggtcg ggggcgacgc
      781 cctccccgcc cgctatcctg ccgtagcgcc gcgaaagcgc ctgcgccagg gcgccgtagt
      841 cgtcgcccgg ctgcacgcct tcgatgttgt agcgacggta ttcgccccgg gacatgtcca
      901 tgcgatcgta caccacgcag gccgccaccg tggcctcgcc catcgtgtgg ctcacgtcga
      961 agcattcgat gcgctgcacg gtgtcgggca ggcccaacgc ctcgcgcagc gccgcgagcc
     1021 gggtttcctg agtgctgtgc tcggtgagct tcgacttcag ggcctggagc gcgttttgcc
     1081 gcgccatctc gagccaagcg cggcgctccg ccgtgggacg cgtgtgaacg cgaacgctgc
     1141 gtttcgcgtg ctcgctgagc gtggcttcga gctcttgcac gtccaggtct tcgccgacga
     1201 ccagggccgg cggtatcggc cgttccaggt agtgctgcgc gatgaacgcc gagagcactt
     1261 ccttggcgtc gcgctcctcc gcattctgcg gaaaaaggct gcggtctccg agttgcctgc
     1321 cgccgcgcac catcaccagg ttgacgcaag cgatgccgtg ctccatcgcg accgcgatga
     1381 tatcgacatc gacgtccgcg ccggtatcgg cgtattgacg ctggctgacc ttggcgaggg
     1441 attggatctg gtcgcggtag atcgccgcct cctcgtagcg ccgcgcgtcg gaagcctgct
     1501 gcatgcgggc ctcgagcctc ttgagggcgg tgtcgctgcg tccctgaagg tagagctgcg
     1561 cactcttgac gtcttccgca taggcggcgg cggagatgtg ccccacgcaa ggcgcggtgc
     1621 agcgcctgat ctggtagaga aggcagggcc tcgagcggtt ctggaagacc gagtcttcgc
     1681 aggtgcgcag tcggaacacc ctttgcagca gctggatgct ctcgcgcacc gcacccgcgt
     1741 gcggaaacgg accgaagtag tggtgggcct tgtccatcgc cccgcggtga aagccgagtc
     1801 gcgggaactc cccgccgctc accatcaggt agggatagga cttgtcgtcg cggaacagga
     1861 tgttgtagcg aggcgcaagc gacttgatca gattgttctc gaggatcagc gcctccgctt
     1921 cggagcgggt taccgtgatc tcgaccgccg cgacctgcgt cagcatcatg gcgatgcgcg
     1981 ggccgtgctg cgttttctgg aaataggacg agacgcgctt tttcagatcg cccgccttgc
     2041 cgacatagag cgcctcg
//
LOCUS       DASPZD010000185         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_355011, whole genome shotgun sequence.
ACCESSION   DASPZD010000185 DASPZD010000000
VERSION     DASPZD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355011"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1149)
                     /locus_tag="VEM33_03610"
     CDS             complement(184..1149)
                     /locus_tag="VEM33_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS50943.1"
                     /translation="MNRARRVLSALLCFGAAHTTFAQYPARPIRLVVPFPAGESVDAT
                     ARLVGQSWSAALGQQILVDNRGGAGGTIGSEAVARSAPDGYTLLWGNVGPLAIGPSLY
                     KKLGYDVAKDFEPVSLVATLPFVLFASPVLPANSVVELLAYARAHPGELNFGSTGVGS
                     GLHLIGELFKSEAAIQIVHVPYKGVAQALPEMMSGKVQIAFNTIPAFLPHVKSGRLKA
                     LVITATLRSPLLPDVPTTPEVGLPGVLGASWHAVVAPAGTPREIVSRLNRTLVGTIAA
                     PEMRGTLVAQGAAPVGSTPEDLRTFMREEAVKWARVVQSSGARAD"
     gene            1258..1839
                     /locus_tag="VEM33_03615"
     CDS             1258..1839
                     /locus_tag="VEM33_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50944.1"
                     /translation="MNISDSRQYLGIFFAAAFALALTACFGGKTNVESDLGIKGAPDW
                     VNKGTAVLNDKDGRLFHGVGSSPALGDEALQRSTADDRARAEVARMLSTYLDVVSNDY
                     AAASGTGASASADQSVSRQIKAVTKTNLAGSKIIGRWKDARTNAVYSIAELDLKQVKT
                     TLESANGMNEDLRRYIGRNAENIFDKVSGEKAK"
     gene            1836..2444
                     /locus_tag="VEM33_03620"
     CDS             1836..2444
                     /locus_tag="VEM33_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HYS50945.1"
                     /translation="MSHRPSLRIGLALLALVTVTACSTQVERLQATDVKDLSGGWNDT
                     DSRLVSEEMIRDSLSRPWLQEWGAARGSKRPALIVGEVRNLSHEHINVNTFTLDVERA
                     LINSGKIDFVATKDERKGIREERKDQDINAREDTRNAMGRELGADFIMTGTINTIVDP
                     NGDTQVLFYQVNMTLISLVDNRKVWVGQKEIKKLVTRSKLRF"
     gene            2467..>3532
                     /locus_tag="VEM33_03625"
     CDS             2467..>3532
                     /locus_tag="VEM33_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50946.1"
                     /translation="MSACSVAARAQLPCAAACLAALAACATFSDTIQSTEHSLAQQRP
                     GAALIEYEKLKPVPADRVLYLMNRGMLLRMSGDYDESTRTLEETKRLSEQLRALSLRE
                     QALSFTVNDASKSFVGEDFERVMVNNYLALNYLERGQLDAARVEALQVDLLLRERRQR
                     SSADNPYAEDAFARYLTGIVYEDEGEWSDAMIAYRKAYEAYQKQLQSFAVAIPETLKH
                     DLIRLADRMDLAEEAKGYREEFKIAETLSAADLRERGEIILTVHTGLAPNKRERATTV
                     PNPATGRILRIALPQYRSRAQPFAYASVSADLNSTKTSLVENIDAIAVKTLESEMPAI
                     TARAIARMVAKDILASAAASN"
BASE COUNT          682 a         1188 c         1123 g          539 t
ORIGIN      
        1 ccgtaccgag caccacggtg gttttttgct gtgccgcagc tttgtccatg ccggcgccca
       61 tcatcgcggc cagggcgagc gcagcgacgg cgagttctcg aaaacgatgg tccatggtct
      121 ctccctcatt gcttttttga ttaccgatcg gaatccccga ttactctacc acgcgcccga
      181 cgctcaatcg gcgcgcgcgc cggaggactg gaccacgcgg gcccacttca ccgcctcttc
      241 ccgcatgaag gtcctcaagt cctccggagt gcttccgacg ggcgccgcac cctgggccac
      301 gagagtaccg cgcatctcgg gcgcggcgat cgtgcccact agcgtccggt tcagccggga
      361 gacgatttcg cggggcgtgc ctgccggcgc gaccacggcg tgccaggacg caccgagcac
      421 ccccgggagc cccacttcgg gcgtcgttgg gacgtcgggg agcaggggcg agcgcagggt
      481 cgcggtgatg acaagcgcct tcaggcgccc ggatttcacg tgcggcagga acgcgggaat
      541 ggtattgaag gcgatctgca ccttgccaga catcatctcg gggagcgcct gcgcgacgcc
      601 cttgtaggga acgtgcacga tctgtatggc cgcttcgctc ttgaagagct cgccgatcag
      661 atgcaggccc gacccgactc cggtcgagcc gaaattcaac tcgccgggat gcgcgcgggc
      721 gtaggcgaga agctcgacca cggaattcgc cggcagcacc gggctcgcga agagcacgaa
      781 aggcaaggtc gcaaccagcg acaccggctc gaagtccttc gcgacgtcgt agccgagctt
      841 cttgtagagg ctcggcccga tggcgagcgg cccgacgttg ccccacagga gcgtataacc
      901 gtcgggggcg gatctggcca cggcctcgct gcctatggtc ccgcccgcgc cgccgcggtt
      961 gtccaccagg atctgctgtc cgagggccgc cgaccacgac tgtccgacga ggcgcgcggt
     1021 cgcatccacc gattcgcccg ctggaaaagg cacgacgagg cggatcggtc ttgcgggata
     1081 ttgcgcaaag gtggtgtgcg cggcaccgaa acacagcagg gcggaaagaa ctctcctagc
     1141 tcgattcatt accattacct ttcaagccgg aggcgcagtg cctgtgtgct gggacggcat
     1201 ggtattctat ccggagatcg tccagcagct ctgcggtact tcatacagga ggaccccatg
     1261 aacatttccg actcacgcca gtacctcggg attttcttcg cggccgcctt tgcgctcgct
     1321 cttaccgcgt gcttcggcgg caagaccaac gtcgagagcg acctcggcat caagggcgcc
     1381 cccgactggg tcaacaaggg caccgcggtc ctcaacgaca aggacggcag gctgttccac
     1441 ggcgtggggt cctcccccgc gctgggcgac gaagccctgc aacgctccac cgcggacgac
     1501 cgcgcgcgcg ccgaagtcgc gcgcatgctc tcgacctatc tcgacgtggt cagcaacgac
     1561 tacgccgcag cgtcgggcac cggcgccagc gccagcgccg accagtccgt gtcgcgccag
     1621 atcaaggcgg tcaccaagac gaatctcgca ggctcgaaga tcatcggccg ctggaaggac
     1681 gccaggacca acgcggtcta ctcgatcgcc gagctcgatc tgaaacaggt aaagaccacg
     1741 ctcgagagcg ccaacggcat gaacgaagac ctgcgccgct acatcggccg caacgccgag
     1801 aacattttcg acaaggtgtc cggggagaaa gcaaaatgag ccaccgtcca tctctgcgca
     1861 ttggccttgc gctgctcgcg ctcgttacgg tcaccgcctg ctcgacccag gtcgagcgcc
     1921 tgcaggcaac cgacgtcaag gacctgagcg gcggttggaa cgacaccgat tcgcgtctcg
     1981 tgtccgagga aatgatccgc gactcgctgt cgcggccctg gctgcaggag tggggcgctg
     2041 cccggggctc gaagcggccc gcgttgatcg tcggcgaagt gcgcaacctg agccacgagc
     2101 acatcaacgt caacaccttc accctggatg tcgagcgcgc gctgatcaat tccggcaaga
     2161 tcgatttcgt cgccaccaag gacgagcgca aggggatccg cgaggagcgc aaggaccagg
     2221 acatcaacgc gcgcgaggac acgcgcaacg cgatgggacg cgagctcggc gcggatttca
     2281 tcatgaccgg cacgatcaat accattgtcg accccaacgg ggacacgcag gtgctcttct
     2341 accaggtcaa catgaccctc atcagcctcg tcgacaaccg caaggtctgg gtcgggcaaa
     2401 aggaaatcaa gaaactcgtc acgcgcagca agctgcgatt ctagcgcccg gctttaagcg
     2461 aaggtcgtgt ctgcgtgcag cgttgcggcg agagctcagt tgccgtgcgc ggcggcgtgc
     2521 ctcgccgcgc tcgccgcgtg cgcaaccttc agcgacacta tccagtccac cgagcacagc
     2581 ctcgcccagc agcggcccgg ggccgcgctg atcgagtacg agaagctcaa gccggtacca
     2641 gcggaccgcg tcctctacct gatgaacagg gggatgctgc tgcgcatgtc cggggactac
     2701 gacgagagca cgcgcacgct cgaggaaacc aagcgcctga gcgagcagct gagggcgctc
     2761 tccctgcgcg agcaggcgct ctcgtttacc gtcaacgacg ccagcaagag cttcgtcggc
     2821 gaggacttcg agcgcgtcat ggtcaacaac tatctcgcac tcaactacct ggagcgcggc
     2881 cagctcgacg ccgcacgcgt cgaggcgctg caggtcgacc tgctgctgcg cgagaggcgc
     2941 cagcggtcct cggccgacaa tccctacgcc gaggatgcct tcgcgcgcta tctcaccggc
     3001 atcgtgtacg aggacgaggg cgagtggagc gacgcgatga tcgcctaccg caaggcctac
     3061 gaggcctacc agaagcagct ccagtcgttc gcggtggcga tacccgagac gctgaaacac
     3121 gacctgatac gcctcgccga ccgcatggac ctcgccgagg aggccaaggg ctaccgcgag
     3181 gagttcaaga tcgccgagac gctgagcgcg gccgacctgc gcgagcgtgg cgaaatcatc
     3241 ctcaccgtgc acaccgggct cgccccgaac aagcgcgagc gtgcgaccac cgtgcccaac
     3301 ccggcgaccg gacgcatact caggatcgcg ctgccgcagt accgctcgcg ggcccagccg
     3361 ttcgcctacg cgagcgtaag cgcggacttg aactcgacga agacttcgct ggtggagaac
     3421 atcgacgcga tcgcggtgaa gacgctcgaa tccgagatgc cggcgatcac cgcccgggcg
     3481 atcgcccgga tggtggccaa ggacattctg gcgagcgcgg ctgcctcgaa tt
//
LOCUS       DASPZD010000186         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1359919, whole genome shotgun sequence.
ACCESSION   DASPZD010000186 DASPZD010000000
VERSION     DASPZD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359919"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="oxc"
                     /locus_tag="VEM33_03630"
     CDS             complement(<1..1088)
                     /gene="oxc"
                     /locus_tag="VEM33_03630"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HYS50947.1"
                     /translation="MTDGFQLVIDALKLNGLDTIYGLPGIPITDLTRRAQAAGLRVLS
                     FRHEQSAGYAASIAGFLTQKPGICLTVSAPGFLNGLNALAHATVNCFPMILISGSSER
                     EIVDLQQGDYEEMDQLAIAKPVAKAAFRVLHPEDIGVGVARAIRAAVSGRPGGVYLDL
                     PAKLFAQSIDAAAGKNSLIKVVDPAPRQIPAPEAVKRALDLLKSAKKPLIILGKGAAY
                     AQADADIKSLIEKTGIPYLPMSMAKGLLPDTHDLCASAARSFVLPEADVVMLVGARLN
                     WLLSHGKGKTWGGKGHKDWGGQKFIQVDISPQEADSNVRIDAPLVGDIGSGVSALLSG
                     IGSGWAKPPAEWTGAISERKSKNVAKMA"
     gene            1296..1901
                     /locus_tag="VEM33_03635"
     CDS             1296..1901
                     /locus_tag="VEM33_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYS50948.1"
                     /translation="MSAIVNHPQRNTIRLQLKQNLILREMPASAWNDLEPLLEVAEYR
                     KGEPLVRQGTTSMEQFFILDGILKRVVSNPQGKEMILRFAAETDMDTSYAAWRMKTPI
                     PYSIAAVTKVRVARLSMPHWVEFLERHIEMKLRFEYEVMRLMSEVMAHTITLHLLDAP
                     GRLARFMRKHQDLLGRIPKKELASYLNLTPETLSRLRQRAA"
     gene            complement(1914..>2367)
                     /locus_tag="VEM33_03640"
     CDS             complement(1914..>2367)
                     /locus_tag="VEM33_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HYS50949.1"
                     /translation="FQEKPDFVWGLIASMYTGNFIGVLMVLAFVPLFAAILRIPFAIL
                     TSVIVVICAVGSYAVHSNMIDIWYTLIFGVVGYAFKKLDYPLAPLVLALVLGDRAEEN
                     FRNAIKGSQGDLLVFFSNGLVGGLTSLALLLLFWPLIREGWRVVLGKS"
BASE COUNT          436 a          771 c          752 g          408 t
ORIGIN      
        1 tcggccatct tggcgacgtt cttgctcttg cgctcgctga tcgcgccggt ccactccgcc
       61 gggggcttcg cccagcccga gccgatgccg gagagcagtg ccgagacacc tgagccgatg
      121 tcgccgacca gcggcgcatc gatgcgcacg ttgctgtccg cctcttgcgg cgagatgtcg
      181 acctggatga atttctggcc gccccagtcc ttgtggccct tgccgcccca ggtcttgccc
      241 ttgccgtgcg atagcaacca gttcaggcgc gcgccgacca gcatcaccac gtccgcctcg
      301 ggcagcacga acgagcgcgc ggcggatgcg caaagatcgt gcgtgtcggg cagcagaccc
      361 ttcgccatcg acatcgggag gtacggaatc ccggtctttt cgatcaggga cttgatgtcc
      421 gcgtcggcct gggcgtaggc ggcgcccttg ccgaggatga tcagcggctt cttcgcgctc
      481 ttcagcagat cgagcgcgcg cttgaccgcc tcgggagccg ggatctggcg cggggccgga
      541 tcgacaacct tgatcaacga gttcttcccg gccgcggcgt cgatgctctg ggcgaagagc
      601 ttcgccggga gatcgagata cacgccgcct gggcggccgg agaccgcggc gcggatggcg
      661 cgagcgacgc cgacgccgat gtcctcgggg tgcagcacgc ggaacgccgc cttggcgacg
      721 ggcttggcga tcgcgagctg gtccatctcc tcgtagtcgc cctgctgcag atcgacgatt
      781 tcccgctcgc tggagccgct tatcagaatc atcgggaagc agttgaccgt ggcatgcgcg
      841 agcgcgttga ggccgttgag gaagccgggc gccgacaccg tcaggcagat gccgggcttc
      901 tgcgtcagga agccagcgat cgaggcggca tagccggcgc tctgctcgtg gcggaacgaa
      961 agcacgcgca ggcccgcggc ctgcgcgcgg cgcgtcaggt cggtgatcgg gattccgggc
     1021 aggccgtaga tcgtgtcgag gccattaagc ttgagggcat cgatgaccag ctggaaacca
     1081 tcggtcatct cctgcgattg cgcatcggcg acgatctgac cggtcgcgac ctgcggctcg
     1141 actgttccca tgatggtctc cttgcgtgca gtgcccggcc ggataagcgc cagggccgaa
     1201 tcgaactcaa agtctaggat tgttaaggac ctttccacct tgactgcgat caataattcc
     1261 cgcttaattg agccctttcg cggttagtcc tgcccgtgtc ggcgatcgtc aatcacccgc
     1321 agcgcaacac catccggctg cagctcaagc agaaccttat cctgcgcgaa atgccggcgt
     1381 ccgcgtggaa cgatctggag cctttgctgg aggtcgcaga gtacaggaag ggcgagccgc
     1441 tggtgcgcca aggcaccacc agcatggagc agttcttcat cctggacggg atcctgaagc
     1501 gcgtcgtgag caatccccag ggaaaagaga tgatcctgcg cttcgcggcc gagaccgaca
     1561 tggacacctc ctacgccgcg tggcggatga agacccccat cccctacagc atcgcggcgg
     1621 tcaccaaggt ccgcgtggcg cgcctgtcca tgccccactg ggtcgagttc ctcgaacgcc
     1681 acatcgaaat gaagctgcgc ttcgaatacg aggtcatgcg gctgatgtcc gaggtgatgg
     1741 cgcatacgat cacgcttcac ctgctcgacg cccccggtcg ccttgcgcgc ttcatgcgca
     1801 agcaccagga cctgctcggc cgcataccca agaaagagct ggcgtcatac ctcaacctga
     1861 ccccggaaac cctgtcccgc ctcaggcagc gggccgcctg agcgcgctcg ttttcagctt
     1921 tttccgagca ccacgcgcca cccctcccga atgagcggcc agaagagcag cagcaaggcg
     1981 agactggtca ggcctcccac cagtccgttc gagaaaaaga ccagaaggtc gccctgggag
     2041 cctttgatcg cgttgcggaa attttcctcc gcccggtcgc ccagaacgag cgccagcacc
     2101 agcggggcga gcggatagtc gagcttcttg aacgcgtagc cgacgacgcc gaagatcagc
     2161 gtgtaccaga tgtcgatcat attgctgtgc accgcgtagg agccgaccgc gcaaatgacc
     2221 acgatcactg acgtgaggat cgcgaacggg atccggagga tcgccgcgaa gagcggcacg
     2281 aaggccagga ccatcaggac gccgatgaag ttaccggtgt acatgctggc gatgagcccc
     2341 cagacgaagt cgggcttctc ctggaac
//
LOCUS       DASPZD010000187         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_139621, whole genome shotgun sequence.
ACCESSION   DASPZD010000187 DASPZD010000000
VERSION     DASPZD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139621"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VEM33_03645"
     CDS             <1..835
                     /locus_tag="VEM33_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYS50950.1"
                     /translation="FSEERAKRQIKREFNLTYLSALPADNKLSEGAWFGAEDIARGAV
                     SIEHWIAERFGIGVGDTLEFLSAGRSFSAPVTSVRKLDWDSMRPNFFFITTPAMLESF
                     PASYIASFHAPAGNALLTLRLSQAFPNLTVIDVSMILRQLQLVMDQLIGAVQLVFLFA
                     LGAGVLVLYAALLATQDERTHEAAVMRALGASRAQVLAAQRAEFAVLGATAGLLGALG
                     ATAIGWTLATRVFQLDFIWNGWVWLAGPALGLACIVLNAWAGARAALGRPPMAALREV
                     I"
     gene            860..1981
                     /locus_tag="VEM33_03650"
     CDS             860..1981
                     /locus_tag="VEM33_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HYS50951.1"
                     /translation="MAEIALTIAGASLVVLVWLAFGFARLRSSLKDPVAAVAPLLEEK
                     HRAMLTDLHNGLTHQGDRLGRNLADSSERLSTKIDQRLDQIAGKVDERLDEGFRKTNE
                     TFVNVMQRLATIDEAQKKIESLTGSVVSLQELLGDKRSRGAFGEVQLEALVRNVLPPM
                     AFELQYTLSNGMRVDCALRLPPPTGLVTVDSKFPLENYHRMFDREADQTERTLAQKQF
                     KADIRKHVDDIARKYIIAGETSDGAVMFVPAEAVFAEIHAYHPEVVEYAIGRRVWIVS
                     PTTLMAVLNTARAVLKDVETRRHIHIIKEELGKLGIDFQRFDERMKKLADHIRQAHED
                     AEDVQTSSRKISQRFQQIEAAEIEEKPPANVVRLEPDRK"
BASE COUNT          399 a          700 c          720 g          390 t
ORIGIN      
        1 cttttcggaa gagcgcgcga agcgccagat caagcgcgag ttcaacctga cctatctgtc
       61 tgcattgccg gccgacaaca agctcagcga gggcgcgtgg ttcggtgcgg aggatatcgc
      121 gcgtggcgcg gtctcgatcg agcactggat tgccgagcgt ttcgggatcg gggtcgggga
      181 tacgctcgaa tttctctcgg cgggccgcag cttctccgcg ccggtcacga gcgtgcgcaa
      241 gctcgattgg gactcgatgc gcccgaattt cttcttcatc accactccag caatgctcga
      301 gagcttcccg gcgagctaca tcgcgagttt tcatgcgcct gcaggcaacg cgctcctgac
      361 cttgcgcctg tcgcaggcgt ttcccaatct cacggtgatc gacgtaagca tgatcctgcg
      421 gcagctacag ctcgtgatgg accagctgat cggcgcggtg cagctggtgt tcctgttcgc
      481 actcggcgcc ggcgtgctag tcctgtacgc ggcgctgctc gcgacccagg acgagcgtac
      541 gcacgaggcg gccgtgatgc gcgcgctggg ggcgagccgc gcccaggtgc tcgcggcgca
      601 gcgcgccgag ttcgctgtgc tcggtgcgac cgcagggctc ctcggcgcgc tcggtgcgac
      661 cgcgatcggc tggacactcg cgacgcgcgt cttccagctc gatttcatct ggaacggttg
      721 ggtctggctc gccggccccg cgctcgggct cgcgtgcatc gtgttgaacg cctgggcagg
      781 ggcccgcgcg gcactcggcc gcccgccgat ggcggcgctg cgcgaggtca tatgaagaga
      841 ccctgcgtta caatggctca tggctgaaat cgcactcact attgccggcg caagcctcgt
      901 cgttctcgtc tggcttgcgt tcggcttcgc ccgcctgcgt agctcgctca aggaccccgt
      961 tgccgcggtc gcgccgcttt tggaggagaa gcaccgcgcc atgctcacgg atctgcataa
     1021 cgggcttacc catcagggtg accggctcgg gcgcaacctc gccgattcga gcgagcggct
     1081 ctcaaccaag atcgaccagc gcctggacca gatcgctggc aaggtcgacg aacgcctcga
     1141 cgagggtttt aggaaaacca acgagacctt cgtcaacgtg atgcagcgtc tggcgaccat
     1201 cgacgaagcc cagaagaaga tcgagagcct gacgggcagc gttgtgagcc tccaggagct
     1261 gctcggggac aagcgcagcc gcggggcgtt cggcgaggtg cagctcgagg ccctggtgag
     1321 gaacgtgctg ccgcccatgg cgttcgagct gcagtacacc ctttccaacg gcatgcgcgt
     1381 cgattgcgcg ctgcgccttc cgccgcccac aggactggtg acggtggatt caaagtttcc
     1441 cctggagaac taccaccgca tgttcgaccg cgaggcggac cagacggagc gcacgctcgc
     1501 gcaaaaacag ttcaaggccg atatccggaa gcacgtcgac gacatcgcga ggaaatacat
     1561 catcgcgggc gagacctcgg acggcgctgt gatgttcgtc ccggcggagg cggtgttcgc
     1621 cgagatccac gcctaccatc ccgaggtcgt cgagtacgcg atcggcaggc gcgtgtggat
     1681 cgtctccccg accacgctga tggctgtgct gaatactgcg cgtgctgtgc tgaaagacgt
     1741 cgagacgcgc aggcacatcc acatcatcaa ggaggaactc ggcaagctcg gcattgattt
     1801 ccagcgcttc gacgagcgca tgaagaagct cgccgatcac atccgccagg cgcacgagga
     1861 tgccgaggat gtgcagacct ccagccgcaa gatctcgcag cgcttccagc agatcgaggc
     1921 ggcggaaatc gaggagaaac ccccggccaa cgtggtgcgg ctggagcccg acaggaagtg
     1981 attcgagggc gagaaagagc aggctgaaaa aaatgcccgc aactcccgtt gcgggcattg
     2041 tgattgctgt cctgctaatc aatcattgtc atcggcgttg ccgtggtcgt cggtgcggct
     2101 gatcttccag atcgtcccgg tgcccgggat ctgcacgagc ggcgcattgg ccggattgac
     2161 aaaccgcgaa tccggatccg aaccgcccgg atcgcgcacc gcaccgtag
//
LOCUS       DASPZD010000188         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1705585, whole genome shotgun sequence.
ACCESSION   DASPZD010000188 DASPZD010000000
VERSION     DASPZD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705585"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /gene="uvrA"
                     /locus_tag="VEM33_03655"
     CDS             <1..1914
                     /gene="uvrA"
                     /locus_tag="VEM33_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYS50952.1"
                     /translation="KGWDRRNQFYFQMLTSLAAHFRFDVEAPFEKLPEPVQALVLHGT
                     QERIPFSYLSERGRTVVKEHAFEGILPSLERRYKETDSLVVREELAKYLNTRACPSCG
                     GTRLRPEARHVKIDDRAIFEVSAFPMKQALEFFRRLKLEGSKQAIAEKIVNEIIARLN
                     FLNNVGLDYLSLDRAADTLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNAR
                     LLATLKHLRDIGNTVIVVEHDEEAINTADHVLDMGPGAGEHGGQVVAEGTPEQIRRNP
                     ASLTGQYLCGALEIAVPKKRRRFDRARVLRLKGARGNNLKNIELALPVGLFVCVTGVS
                     GSGKSTLINDTLYHAVARELYGSTHEPAAHDSVKGLEFFDKAVSVDQSPIGRTPRSNP
                     ATYTALFTPIRELFAGVPMARERGYGPGRFSFNVKGGRCEACQGDGVIKVEMHFLPDI
                     YVACDTCRGKRYNRETLEVQFKGKNIHEVLQMTVAAAHEFFNTVPAVERKLQTLLEVG
                     LGYIQLGQPATTLSGGEAQRVKLSLELSKRDTGRTLYILDEPTTGLHFKDIDLLLSVL
                     HRLRDAGNTVVVIEHNLDVIKTADWSIDLGPEGGDGGGRIVAQGTPEDLAAIDASFTG
                     QTLRNVLGVKRAA"
     gene            complement(1916..>2528)
                     /locus_tag="VEM33_03660"
     CDS             complement(1916..>2528)
                     /locus_tag="VEM33_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HYS50953.1"
                     /translation="YVQLSGVLKRIAVKLSKVCNDLRLLSSGPRAGLGEINLPPVQAG
                     SSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEAGQLQLNAFEPVIAHSLFKSLMH
                     LQNGCDTLRERCVTGITANRERMAQTVRESIGLVTALNPYIGYSAASEVALEALATGR
                     AVYDLVLEKKLLSKEQLDEILRPENLTRPGRPVPPEPSRARKA"
BASE COUNT          456 a          876 c          808 g          388 t
ORIGIN      
        1 aagggctggg accggcgcaa ccagttctat ttccagatgc tcacgagcct cgccgcgcat
       61 ttccggttcg acgtcgaggc gccgttcgag aaactccccg aaccggtcca ggcgctcgtg
      121 ctccacggca cgcaggaaag gatcccgttc tcctacctga gcgagcgcgg ccgcacggtg
      181 gtgaaggagc acgccttcga gggcatcctg cccagcctcg agcgccgcta caaggaaacc
      241 gattccctgg tcgtgcgcga ggagctcgcg aaatacctga acaccagggc ctgcccctcg
      301 tgcgggggaa cgcggctccg cccggaggcg cggcacgtca agatcgacga ccgcgcgatt
      361 ttcgaggtga gcgctttccc catgaagcag gcgctcgaat tcttccgccg gctcaagctc
      421 gaaggcagca agcaggccat cgccgagaaa atcgtcaacg agatcatcgc gcggctcaac
      481 ttcctcaaca acgtcggcct cgactatctg tcgctcgacc gcgccgcgga tacgctctcg
      541 ggcggcgagg cgcagcgcat ccgcctcgcg agccagatcg gctcgggact caccggcgtc
      601 atgtacgtcc tcgacgagcc ctcgatcggc ctgcaccagc gcgacaacgc ccgcctcctc
      661 gccacgctca agcacctgcg cgacatcggg aacaccgtca tcgtggtcga gcacgacgaa
      721 gaggcgatca acacggccga tcacgtgctc gacatgggac cgggcgcggg cgagcacggc
      781 ggccaagtgg tcgcggaagg cacgcccgag cagatccgca ggaaccccgc ctccctcacc
      841 ggccagtacc tatgcggcgc gctcgagatc gccgtgccca agaaacgccg ccgcttcgac
      901 agggcgcggg tgctgaggct gaaaggcgcg cgcgggaaca atctcaagaa tatcgagctc
      961 gcgctgccgg tgggtctgtt cgtgtgcgtc accggcgtct cgggctcggg taaatcgacg
     1021 ctcatcaacg acacgctcta tcacgccgtc gcgcgcgagc tctacggctc gacccacgag
     1081 cccgccgcgc acgattcggt caaggggctg gaattcttcg acaaggcggt gagcgtcgac
     1141 cagagcccga tcggccgcac ccctcgctcc aaccccgcca cttacacggc gctgttcacg
     1201 cccatccgcg agcttttcgc gggcgtgccg atggcgcgcg agcgcggcta cgggccgggg
     1261 cgcttctcct tcaacgtgaa gggcgggcgc tgcgaggcct gccagggcga cggcgtcatc
     1321 aaggtcgaga tgcatttcct gccggacatc tacgtggcct gcgacacctg ccgcggcaag
     1381 cgctacaacc gcgaaaccct ggaggtgcag ttcaagggaa agaacatcca cgaagtgctg
     1441 cagatgaccg tagcagccgc ccacgagttc ttcaatacgg tccccgcggt ggagaggaag
     1501 ctccagaccc tgctcgaagt gggcctgggc tatatccagc tcggccaacc ggcgaccacc
     1561 ctctccgggg gcgaggcgca gcgggtgaag ctatcgctcg agctctccaa gcgcgacacc
     1621 ggacgcacgc tctacatcct ggacgagccg accacgggcc tgcacttcaa ggacatcgac
     1681 ctgctgctct cggtgctgca ccgcctgcgg gacgcgggca ataccgtggt cgtcatcgag
     1741 cacaacctcg acgtgatcaa gaccgccgac tggagcatcg acctggggcc cgagggcggc
     1801 gacggcggcg ggcgcatcgt cgcgcaaggc acgcccgagg acctggccgc catcgacgcg
     1861 agcttcaccg gccagaccct gcgcaacgtc ctgggcgtga aacgcgcggc ctaacctatg
     1921 ccttcctcgc ccgcgacggc tcgggcggca ccgggcgccc gggccgcgtc aggttttccg
     1981 gccgcaggat ctcgtcgagc tgttccttgg aaagcagttt tttttccaga accaggtcgt
     2041 atacggccct gccggtcgca agcgcttcca gcgcgacctc actcgccgcg gagtagccga
     2101 tatagggatt gagcgcggtg acgagaccga tcgactcccg caccgtctgc gccatgcgct
     2161 cccggttcgc ggtgatgccg gtgacgcagc gctcgcgcag ggtgtcgcag ccgttctgaa
     2221 ggtgcatcag gctcttgaac aggctgtggg cgatgacggg ctcgaaggcg ttcagttgca
     2281 gctgccccgc ttccgcggca aagctcacgg tcacgtcgtt gccgatgacc tcgaacgcga
     2341 tctggttcac gacctccgga atgaccggat tgaccttgcc gggcatgatc gacgatcccg
     2401 cctgcaccgg cggcaggttg atctcgccga ggccggcgcg gggacccgag gacagcagcc
     2461 gcaggtcgtt gcagaccttg gagagcttca ccgcgatccg tttcagcacg cccgagagct
     2521 gcacgtag
//
LOCUS       DASPZD010000189         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_19952, whole genome shotgun sequence.
ACCESSION   DASPZD010000189 DASPZD010000000
VERSION     DASPZD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19952"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VEM33_03665"
     CDS             complement(<1..220)
                     /locus_tag="VEM33_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYS50954.1"
                     /translation="MNRYPLWVYVTIGVALVLGALYTLPNFFGEAPAVQVSPARATLK
                     VDSTVLGRVEEALGKAGIQPTGITLDLAS"
     gene            complement(221..547)
                     /gene="yajC"
                     /locus_tag="VEM33_03670"
     CDS             complement(221..547)
                     /gene="yajC"
                     /locus_tag="VEM33_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYS50955.1"
                     /translation="MVLISPAWAQAPAGASGPGYEQFFIIIGMFALLYFLMIRPQMKR
                     SKEHKQMTEALQKGDEVVTSGGVLGKITKIGEAYVSIEIAPGTEVQLQRSAIQTLLPK
                     GTIKSI"
     gene            complement(601..1707)
                     /gene="tgt"
                     /locus_tag="VEM33_03675"
     CDS             complement(601..1707)
                     /gene="tgt"
                     /locus_tag="VEM33_03675"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HYS50956.1"
                     /translation="MKFAVHATDGAARAGTLELAHGSVETPVFMPVGTYGAVKAVSPA
                     ELAENGTRIVLGNTFHLWLRPGLEVIAAHGGLHRFIGWRGPILTDSGGYQVFSLGDLR
                     KISEEGVRFASPVNGDKLLLTPEESMRIQRVLDADIAMVFDECTPYPASEGDTAESMR
                     LSLRWAERSKRAHEGNANALFGIAQGGMIEALRDESLAGLAGIGFDGYAIGGLSVGES
                     KREMKRVIAHTAPRLPADKPRYLMGVGTPEDIVEAVTAGIDMFDCVLPTRNARNGWLF
                     TRYGDVKIRNARYRDDTRPLDGTCPCYTCAHFSRGYLHHLQKVNEILGARLNTLHNLH
                     YYQTLMRELRAAIAAGKLADTLAAFREARRKSGD"
     gene            complement(1704..>2548)
                     /gene="queA"
                     /locus_tag="VEM33_03680"
     CDS             complement(1704..>2548)
                     /gene="queA"
                     /locus_tag="VEM33_03680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HYS50957.1"
                     /translation="KARLFGHKETGGHIEVLIERVLSAHEALAQVHAGRAAKPGSTLR
                     LAGGLDVRVLGRAEEFYHLRFPESGTVLELLERHGSVPLPPYVSRAPRGEDEQRYQTV
                     YACSSGAVAAPTAGLHFDEPLLAALAERGVEFAYVTLHVGAGTFQPVREKNLAKHRMH
                     GERYAIPLATAEAIGETRKRGGSIVAVGTTTVRCLEASAAAHGAPVAGAAETDLFITP
                     GFEFRVVDRLVTNFHLPKSTLLVLVSAFAGIEPIRRAYRHAIGERYRFYSYGDAMLVD
                     KMPG"
BASE COUNT          407 a          815 c          882 g          444 t
ORIGIN      
        1 cgctggcgag atcgagcgtg attcccgtcg gctggattcc ggccttcccg agcgcctctt
       61 cgacgcgtcc caggaccgtc gagtccacct tcagggtcgc gcgcgccgga gacacctgca
      121 ctgccggcgc ctcgccgaag aagttcggca gcgtgtacag ggccccgagc acgagcgcca
      181 ccccgatggt gacgtagacc cagagggggt aacggttcat ctagatcgac ttgatcgtgc
      241 ccttgggcag aagcgtctgg atcgcgctcc tttgcagctg cacctcggtg ccgggcgcga
      301 tctcgatcga cacgtaggcc tcgccgatct tggtgatctt cccgagcacc ccgcccgatg
      361 tgacgacctc gtcgcccttc tgcagcgcct cggtcatctg cttgtgctcc ttggagcgct
      421 tcatctgcgg acggatcatg aggaaataga gcagggcgaa catcccgatg atgatgaaga
      481 actgctcgta gccggggccg gacgcaccgg ccggggcctg cgcccatgcg ggggaaatca
      541 acacgatcgt ctccaatgcg gttcagcgag cgggggagta taccatcggg gaaccgctgt
      601 ttaatctcct gatttcctgc gcgcctcgcg aaatgcggcc agggtgtcgg caagttttcc
      661 tgcggcgatg gcggcgcgca gctcgcgcat aagtgtctgg tagtagtgca ggttgtgcag
      721 ggtattgagc cgcgcgccga ggatttcgtt caccttctgc aggtggtgca ggtagccgcg
      781 cgagaaatgg gcgcaggtgt agcaggggca ggtgccgtcc agcggccgcg tgtcgtcgcg
      841 gtagcgggcg ttgcggatct tgacgtcgcc gtagcgggtg aagagccagc cgttgcgggc
      901 gtttcgcgtc ggaaggacgc agtcgaacat gtcgattccg gcggtgacgg cctcgacgat
      961 atcctcaggt gtaccaacgc ccataagata gcggggcttg tccgcgggaa ggcgcggcgc
     1021 ggtgtgggcg atgacccttt tcatttcgcg cttggactcg cccaccgaaa gcccgccgat
     1081 cgcgtagccg tcgaatccga tgcccgcgag gcccgcgagg gattcgtcgc gcagggcctc
     1141 gatcattccg ccctgcgcga tgccgaaaag ggcgttggcg ttgccttcgt gcgcgcgctt
     1201 ggagcgctcg gcccagcgca gggagaggcg catcgactcc gcggtgtcgc cctcgctcgc
     1261 ggggtagggc gtgcattcgt cgaacaccat ggcgatgtcc gcatccagca cccgctggat
     1321 gcgcatcgat tcctcgggcg tgagcaggag cttgtcgccg ttcaccggtg acgcgaagcg
     1381 aacgccctcc tcactgatct ttcgcagatc gccgaggctg aacacctggt agccgccgga
     1441 gtcggtgagt atcgggccgc gccagccgat aaagcggtgc agcccgccgt gcgcagcgat
     1501 gacctcgagc ccggggcgca gccacaggtg aaaggtgttg ccgaggacga tgcgcgtgcc
     1561 gttctcggcg agctcggcgg ggctcaccgc cttcaccgct ccatacgtac ccaccggcat
     1621 aaacaccggc gtctcgacgc tgccgtgcgc cagctccagg gtgccggcgc gcgcggcgcc
     1681 gtcggtcgcg tgaacggcga atttcatccg ggcatcttat caaccagcat cgcgtctcca
     1741 taggagtaga agcggtagcg ctcgccgatc gcgtggcgat aagcacgccg gatgggttcg
     1801 attcccgcaa aagcggacac cagcacgagc agggtcgatt tcggcaaatg aaaattggtg
     1861 acgaggcgat cgaccacgcg gaactcgaac ccgggggtga tgaacagatc ggtctcggcg
     1921 gcgcccgcga ccggagcgcc gtgggcggcg gccgaggctt cgaggcagcg caccgtggtc
     1981 gttccgaccg cgacgatgct gccgccgcgc ttgcgcgttt ccccgatcgc ttccgcggtc
     2041 gcgaggggga tcgcgtagcg ctcgccgtgc atgcggtgct tcgcgagatt tttctcgcgg
     2101 accggctgga acgtcccggc gcctacgtgc agcgtcacgt aggcgaattc gacgccgcgt
     2161 tcggcaagcg ccgcgagcag gggctcgtcg aagtgcaggc ccgctgtcgg cgccgctacg
     2221 gcgccggagg agcatgcgta caccgtctgg taccgttgct cgtcttcgcc tcgtggcgcg
     2281 cgggagacgt aaggcgggag gggaacgctg ccgtggcgct cgaggagctc gaggaccgtt
     2341 ccgctctcgg gaaaacgcaa gtgatagaac tcttccgcgc ggccgagcac gcgcacgtcc
     2401 agtccgcccg cgaggcgcag cgtgctgccg ggtttcgccg cccggcccgc gtggacctgc
     2461 gcgagcgctt cgtgtgccga aagcacgcgc tcgatcagga cctcgatgtg tccgccggtc
     2521 tccttgtgtc caaacaggcg cgccttga
//
LOCUS       DASPZD010000190         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1103057, whole genome shotgun sequence.
ACCESSION   DASPZD010000190 DASPZD010000000
VERSION     DASPZD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103057"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VEM33_03685"
     CDS             complement(<1..385)
                     /locus_tag="VEM33_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HYS50958.1"
                     /translation="MKLLFDLFPVVLFFVAFKLFDIYTATAAAIAATFLQIGWLKWKR
                     RKVDTMMWISLVIIVVFGGATLALHDETFIKWKPTVLYWLFGVVIAVAQSLFRRNLVR
                     AMLGEQVRMPDGVWTRLNWSWAGFFI"
     gene            531..767
                     /locus_tag="VEM33_03690"
     CDS             531..767
                     /locus_tag="VEM33_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HYS50959.1"
                     /translation="MAEPHIAQKSPYAVELAPGDYWWCRCGGSKSQPFCDGTHKGSEF
                     APVKFGVAKAEKLWLCGCKRTRNPPFCDGTHKTL"
     gene            complement(777..1634)
                     /locus_tag="VEM33_03695"
     CDS             complement(777..1634)
                     /locus_tag="VEM33_03695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYS50960.1"
                     /translation="MLRAIPRGFLAALSAALLVMASAAYAQQKDFKPHVGQQGKDVVW
                     VPTPDEVVERMLNIAQTKPEDYVIDLGAGDGKIAIAAAKKFGARALGVEYNPDMVAHA
                     QRNAQAAGVIGKAQIVQGDIFVTDFTQATVLTLYLLPSLNMKLRPQILAMRPGTRVVA
                     HAFNMEDWEPDETSDVDGRRVYFWIVPASAAGRWALELSGAGGSEKLSLNIDQKFQRI
                     EGVAYLGSIHAGLREARLSGFRISFAYVDNHGVRRDFTGRVSGATMEGSFRTDNGAEG
                     RWTAAKKQG"
     gene            complement(1717..3132)
                     /locus_tag="VEM33_03700"
                     /pseudo
     CDS             complement(1717..3132)
                     /locus_tag="VEM33_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            complement(3182..3637)
                     /locus_tag="VEM33_03705"
     CDS             complement(3182..3637)
                     /locus_tag="VEM33_03705"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYS50961.1"
                     /translation="MADKAVSDFVLPATGGKEFRLSAVKSKLLVLYFYPKDNTPGCTN
                     EGAQFRDFYPQFQKAGCSIAGVSRDSVKSHENFKAKMGFPFELLSDEDEKICNQFGVM
                     KMKNLYGKKVRGIERSTFVLDRDRVLRREWRGVKVPGHAQEVLDFVETV"
     gene            complement(3716..>3815)
                     /locus_tag="VEM33_03710"
     CDS             complement(3716..>3815)
                     /locus_tag="VEM33_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS50962.1"
                     /translation="ADSVQGRALDCGHYIPEEAPAELLGELVKFLS"
BASE COUNT          641 a         1197 c         1230 g          747 t
ORIGIN      
        1 agatgaagaa gcccgcccag ctccagttga ggcgggtcca gacgccatcg ggcatgcgca
       61 cctgctcgcc cagcatcgcg cggactaggt ttcttcggaa cagcgactgg gccacggcga
      121 tgacgacgcc gaagagccag tagaggacgg tgggcttcca cttgatgaag gtctcgtcgt
      181 gcagtgcgag cgtcgccccg ccgaatacga cgatgatgac aaggctgatc cacatcatcg
      241 tgtcgacctt ccggcgcttc cacttcagcc agccgatctg caggaacgtc gccgcgatgg
      301 ccgccgcggt cgccgtgtag atgtcgaaaa gcttgaacgc gacaaaaaac aggacgacgg
      361 ggaacagatc gaagagcaat ttcatcgcgc cattataccg gcgcgggtcc gagcgctccc
      421 ggctccgggc ggaagctcgc acccctcgcg ggatcgacgg ggaccgggtt atacttccgc
      481 ctcggtccca cctggtgaca ggtgcctgcg ccgccccaaa ggagaggaat atggccgaac
      541 cccatatcgc gcagaaatcc ccgtacgcgg tcgagctcgc tcccggcgat tattggtggt
      601 gccgctgcgg gggctcgaaa agccagccgt tttgcgacgg cacgcataaa ggcagcgagt
      661 tcgcgccggt gaaattcgga gtggccaagg ccgaaaagct ctggctgtgc ggctgcaagc
      721 gcacccgcaa cccgcctttc tgcgacggca cccacaagac gctctaggcg cccgctctac
      781 ccctgtttct ttgcggcggt ccagcggcct tccgcgccgt tgtcggtgcg gaacgagcct
      841 tccatggtgg cgccgctgac ccgcccggtg aagtcgcggc gcacgccgtg attgtccacg
      901 taggcgaagc tgatcctgaa tccggagagc cgcgcctcgc gcaagcccgc gtggatcgag
      961 cccagatagg ccacgccctc gattctctgg aacttctggt cgatattcag gctgagcttt
     1021 tccgaaccgc cggcgcctga gagctcgagc gcccagcggc ccgcggcgct cgccggcacg
     1081 atccagaaat agacgcgcct gccgtccaca tcggaagtct cgtccggttc ccagtcctcc
     1141 atgttgaagg cgtgcgcgac gacgcgggtt cccggccgca tggcgaggat ctgcggccgc
     1201 agcttcatgt tgagcgacgg cagaagatac agagtgagca cggtcgcctg ggtgaaatcg
     1261 gtcacgaaga tgtcgccctg gacgatctgc gccttgccga tgacccctgc agcctgcgca
     1321 ttgcgctggg catgcgcgac catgtcgggg ttgtactcga cgccgagagc gcgggctccg
     1381 aatttttttg cggcggcgat cgcgatcttg ccgtcgcccg cgccgagatc gatcacatag
     1441 tcctccggct tcgtctgtgc gatgttcagc atgcgctcga cgacctcgtc cggagtcggc
     1501 acccatacca cgtccttgcc ctgctggccg acatggggtt tgaagtcctt ttgctgggcg
     1561 tacgctgcac tcgccatgac gagcagcgcg gcgctgagcg ccgccaggaa gccccttgga
     1621 attgcacgaa gcatgatgtc ctctttctgt ttgagtggga attgtgcggg aacagcgaac
     1681 cggccaacag cacgcggtcg aagtctattg cgtatcttac agggcttcgg cggcgtggtc
     1741 ggcgagccgc gagcgctcgc cgcgctgcag ggtcacgtgc cccgagtgcg accagccttt
     1801 catgcggtcc acgacatagg tcaggccgga gctgccctcg gtgagatagg gggtatcgat
     1861 ctgcgcgatg ttgcccaggc acacgacctt ggtgccgggg cccgcgcggg tgataagggt
     1921 cttcatctgc ttgggcgtca ggttctgcgc ctcgtcgatg atgaggtact tgttgatgaa
     1981 ggtgcgcccg cgcatgaaat tcagcgactt gatcttgatc ctggagcgga tcaggtcgcg
     2041 cgtggcggcg cggccccatt cgcccgcgtc gtcgtcggtc ttgttgagaa cgtcgagatt
     2101 gtcctcgagc gcccccatcc agggcgccat tttctcctcc tcggtgcccg gcaggaaacc
     2161 gatgtcctcg ccgaccggca cggtgatccg ggtcatgatg atctcggagt agtgcttggt
     2221 ttcgagcgtc tgcataaggc ccgcggccag ggtgagcagc gtcttgccgg tccccgcctg
     2281 gccgagcagc gtcacgaagt cgatttcggg gttcatgagg agattgagcg cgaagttctg
     2341 ctcgcggttg cgcgccgtga tgccccagac gttgttcttg ccgtgggtgt agtcgcgcgc
     2401 ggtctcgagc accgcggttc cgtccgagac ttccttcacc gtggcgagca gcgggcgctc
     2461 cccgtcctga tacacgaact cgttcagcag caggtcccgg cagagcgggc cgtgtacgcg
     2521 gtagtacgtg cgtccctcgc gtttccatga ctcgatgtcc ccgccgtgac tgtcccagaa
     2581 atccgccggc agctcgcgaa cgccggtgta gagcaggtcg gtgtcctcga ggaccttgtc
     2641 gttgtagtag tcctccaccg tcaggcccag cgcccgggcc ttgatgcgca tgttgatgtc
     2701 cttcgacacc agggtcacgc ggcgctccgg gtcgcgcttc tgcaggtaga tcacgacgcc
     2761 gatgatgtgg ttgtcggtct tgccgatcgg cagattggcg ggaatgtgcc cgttgatcgc
     2821 ctcggtctgc aggtagaggc gccccctcgc gttgtcggct tcgtggcgcg cgagcggaat
     2881 cccctccgct atgggcgtct tggcatggct cacgatgtcg tccagaaacc ggctcgcctg
     2941 gcgcgcgttg cgggcgattt cggagatgcc gctcttgttg ttgtcgagct cctcgagcgt
     3001 catgatcggc aggaacacgt cgtgttcctc gaaacggaac aggctcgtcg ggtcgtgcat
     3061 cagcacgttg gtgtcgagaa cgaagagttt cgtcgcggac tgccgggact cggtgcgggg
     3121 gcggcgggaa ggcagggatt tgcgcgaagc tcctgccggg ggcacggaac ggacctgccg
     3181 ctcagacggt ttcaacaaaa tcgagcacct cctgggcatg gcctggaacc ttgactccgc
     3241 gccattcgcg ccggagaacg cggtcgcggt cgagcacgaa ggtgctgcgt tcgatgccgc
     3301 gtaccttttt cccgtacagg ttcttcatct tcatcacgcc gaactggttg cagatctttt
     3361 cgtcttcgtc ggacagcaac tcgaagggaa agcccatctt ggccttgaaa ttctcgtgcg
     3421 acttgacgct gtcgcgcgag accccggcta tcgaacatcc cgccttctgg aactgcgggt
     3481 agaaatcgcg aaactgcgct ccttcgttgg tgcagcccgg ggtgttgtcc ttcgggtaga
     3541 aatagaggac gagcagcttg gatttgaccg ccgagagccg gaactccttg ccgccggtgg
     3601 cgggcaggac gaagtcgctt actgctttgt cggccatcca gaatccgtgc ctctatgggc
     3661 gccattgtcg cccgaattcc gaagcgaacg caactcgact gcgccccgcg gcggatcacg
     3721 aaagaaattt tacgagctcc cccagcaatt ccgccggcgc ttcctccggg atgtaatggc
     3781 cgcaatccag ggcgcgcccc tggacggaat ccgcc
//
LOCUS       DASPZD010000191         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1476053, whole genome shotgun sequence.
ACCESSION   DASPZD010000191 DASPZD010000000
VERSION     DASPZD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476053"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..914
                     /locus_tag="VEM33_03715"
     CDS             39..914
                     /locus_tag="VEM33_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYS50963.1"
                     /translation="MEAFFASLVSVAFHGTALGMILYVISVGLSVTMGLMGFANLAHG
                     VFAMAGGYVLTTAISRFGAPFPLALALAFVFVAAASVVLERLLYSRLYTASDLEQVLF
                     TIGLIFISVAVARFVYGTLQQPVVLPDYLKGQFALLGRDFPAYRVFIIVFSAVMVGLL
                     WFGVERTRWGAMVRATVDNRAMAQSVGIDTRRLFTLTFALGSGLAGLGGGLGAEIIAI
                     QPTYPFENLVYFLVVVAVGGLGSLRGPFVAALLIGIADTACKYWLPQYGAFPIYVATI
                     AILLWRPAGLFGRRA"
     gene            911..1888
                     /locus_tag="VEM33_03720"
     CDS             911..1888
                     /locus_tag="VEM33_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYS50964.1"
                     /translation="MSAHAGGYSADPRVRWIESLPWIVAIAAFFALPEYLSLGARILI
                     YILYALSLDLIVGYAGIITLGHSAYFGLGAYVAGILAAKAGIGDPMVQLAAAAAAGAL
                     LGIGTGTIILRTRALTLLMLTLAITSVLLEIANKATALTGGADGLSGVVVAPIFGLFR
                     FDIFGKTAYLYCLVALLLGWWLVRKMVYSPFGTSLTGVRENSLRMHAIGAPVYWRLVL
                     VYTLSATIAGVAGCLITQTNQFVGLNVFSFESAGELLVMLILGGVGRIYGAFVGPVVY
                     LIAQDLLAKQFPEYWYFGIGTMLVLAVMFARGGVLGILDGVLVKLRRRP"
     gene            1885..>2086
                     /locus_tag="VEM33_03725"
     CDS             1885..>2086
                     /locus_tag="VEM33_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS50965.1"
                     /translation="MNIALETHGLCKNFGALTVADNIDFRLEAGARHALIGPNGAGKT
                     TFVNMLTGVLPPSSGRIVLGGED"
BASE COUNT          295 a          691 c          653 g          447 t
ORIGIN      
        1 gcgtcaagct tgaatagccg caaaacccgg ggagacgttt ggaggccttc ttcgcatcgc
       61 tggtgagcgt cgcctttcac ggcaccgcac tcggcatgat cctgtacgtg atatcggtcg
      121 gcttgtccgt caccatgggc ctgatgggct tcgccaacct cgcgcatgga gtgttcgcca
      181 tggcgggagg ctatgtgttg accaccgcga tctcgcgctt cggcgcgccc tttccgctcg
      241 cactcgcgct cgccttcgtt tttgtcgcag ccgccagcgt ggtcctcgag cggctcctgt
      301 attcgaggct ctacacggcg agcgatctcg agcaggtgct cttcaccatc gggctgatct
      361 tcatctcggt cgccgtggca cgcttcgtct acggaacgct gcagcagccg gtggtgctgc
      421 ccgattatct gaagggccag tttgctctcc tcggccgcga ttttccggct taccgggtat
      481 tcatcatcgt cttcagcgcg gtgatggtgg ggctcttgtg gttcggcgtc gagcgcacgc
      541 gctggggtgc catggtgcgc gcgacggtgg acaaccgcgc catggcgcag tcggtgggaa
      601 tcgacaccag gcgcctgttc acgctcacct tcgcgctcgg cagcgggctc gcgggactcg
      661 gcggcggcct cggcgcggag atcatcgcga tccagcccac ttatccgttc gagaacctcg
      721 tgtattttct ggtcgtggtg gcggtgggcg gcctgggcag cctgcgcggg ccgttcgtgg
      781 cggcactcct gatcggcatc gccgacacgg cttgcaagta ctggctgccg caatacggcg
      841 ctttcccgat ctatgtcgcc accatcgcca tcctgctgtg gcgaccagcg ggcctgttcg
      901 ggagacgcgc atgagcgccc acgccggagg ctacagcgcc gatccgcgcg tccgctggat
      961 cgagtcgctg ccttggatcg tcgcaatcgc ggccttcttc gctctgcccg agtatctctc
     1021 cctcggggcg cgcatcctga tctacatcct ctacgctctt tcgctcgatc tgatcgtcgg
     1081 ctacgcggga atcatcacgc tgggccacag cgcctacttc ggtctggggg cgtacgtcgc
     1141 cgggattctc gccgcaaagg cgggaatcgg cgatccgatg gttcagctcg ccgccgccgc
     1201 agccgccggc gccttgctcg gaatcggcac cggcaccatc attctccgga cccgagcttt
     1261 gacactgctt atgctcacgc tcgcgatcac ctccgtactc ctggagatcg ccaacaaggc
     1321 cactgcgctc accgggggcg cggacggcct gtccggcgtc gtcgtggcgc cgattttcgg
     1381 tctcttcagg ttcgatatct tcggcaagac cgcttatctc tattgcctcg tcgcgctgct
     1441 cctcgggtgg tggctggtga ggaagatggt ctattctccg ttcgggacct cgctcacggg
     1501 ggtgcgcgaa aacagcctgc gcatgcacgc gatcggcgcg ccggtttact ggcgcctggt
     1561 cctggtctac accctctcgg ccacgatcgc cggcgtcgcc ggatgcctga tcacccagac
     1621 caatcagttc gtcggcctga acgtgttctc cttcgaatcc gccggcgagc tgctggtgat
     1681 gctgatcctg ggcggcgtcg gacgcatcta cggcgcgttc gtcgggccgg tcgtgtacct
     1741 gatcgcgcag gatttgctcg ccaagcaatt tccagaatac tggtacttcg gcatcgggac
     1801 gatgctcgtg ctcgcggtga tgttcgcccg cggcggagtc ctcggcattc tcgacggcgt
     1861 cctcgtgaag ctgcggagaa ggccgtgaat atcgctctcg aaacgcacgg tctgtgcaag
     1921 aacttcggcg cactgaccgt tgcggacaac atcgatttcc ggctggaagc cggcgcgcgc
     1981 cacgccttga tcggtccgaa cggcgccgga aagaccactt tcgtcaatat gctcaccggc
     2041 gtgctgcccc cctcctccgg ccgaatcgta ctcggcgggg aagaca
//
LOCUS       DASPZD010000192         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1013682, whole genome shotgun sequence.
ACCESSION   DASPZD010000192 DASPZD010000000
VERSION     DASPZD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013682"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VEM33_03730"
     CDS             complement(<1..476)
                     /locus_tag="VEM33_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYS50966.1"
                     /translation="MPRRARLRIAGAPLHIIQRGNNRSVCFFNNHDYGCYLHYLKELA
                     VRYTCSIHAYVMMTNHVHLLLTPARPDGASLLMKHLGQRYVQYVNRVYDRSGTLWEGR
                     FRSSIVQAETYLLRCQRYIELNPVRAGMVDAPGDYPWSSFGANALGNRGELITPHP"
     gene            complement(531..1409)
                     /locus_tag="VEM33_03735"
     CDS             complement(531..1409)
                     /locus_tag="VEM33_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYS50967.1"
                     /translation="MSRLPAALRGALWMFGALLSFSLMAVAVRELLRSMGSFEILFLR
                     SLVSFVLVLAVLPRFGIGVLRTQRFGLHVVRNVLHFGGQYAWVYAIAMLPLATVFAIE
                     FTMPVWAAVLAMLVLGERLNRGRIVMLVLGLAGILIILRPGFGIVQPAALVMLAGSFA
                     FASTMIATKRLSENDSALAVLFYMSAIQLPLGFVPALQGWVAPTLTDLPWIIAVGGAA
                     SSAHYCMTRAVRIADAMLVIPIDFLRLPLIAVVGMLFYGERLEPPILLGAAVIFAGTY
                     YSIRRESRKPGTDHGL"
     gene            complement(1497..>2074)
                     /locus_tag="VEM33_03740"
     CDS             complement(1497..>2074)
                     /locus_tag="VEM33_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HYS50968.1"
                     /translation="AHPNFYAPAFSWRAMVELVVPLAITVLVVQDGQGITVLRSAGHA
                     PPVDAVTIACGAGSIVTAFVGAVSTCLTGPVNAIISSSGDRRRHYTAGIFVGLLAVAF
                     GLFAPFFTRLMLAAPKAFVAALAGLAMLRVLQTAFAVSFRDRFALGALVTFLVTVADI
                     PVFNIGAPFWGLVFGYAVSRLLERSDFPARG"
BASE COUNT          435 a          644 c          694 g          301 t
ORIGIN      
        1 atgggatggg gagtgatcag ctcaccgcgg ttgcccaacg cgttcgcgcc gaagctcgac
       61 caagggtagt caccgggagc gtcgaccata ccggcgcgca ccggattgag ctcgatgtac
      121 cgctggcaac gcagcaggta ggtttcggcc tgaacgatgc tggaccggaa gcgtccttcc
      181 cacagtgtgc cgctgcgatc gtagacgcga ttaacgtatt gcacgtagcg ctggcccaag
      241 tgtttcatca gcagcgaggc cccatcgggc cgggctggcg tgagcaatag gtgcacgtgg
      301 ttggtcatca tgacataggc gtgtatcgag cacgtgtagc gcaccgccag ctctttcaga
      361 tagtggagat agcagccgta gtcgtgattg ttaaaaaagc agacgctgcg attgtttccc
      421 cgctggataa tgtgcagcgg ggcgccggcg atgcgtaagc gggcgcgacg gggcatgacg
      481 agtgtgtcgg atcctctcga taccctctaa cgcccctgca gccgtcaggc ttacaaaccg
      541 tggtctgtcc ccggttttcg gctttcgcgc cggatgctgt agtaggtccc ggcgaagatc
      601 accgccgctc cgagcaggat cggcggttcc aggcgctcgc cgtagaacag cattcccacc
      661 acggcgatca ggggcaggcg gagaaaatcg atggggatga cgagcatggc gtcggcgatc
      721 cgcacggcgc gcgtcatgca gtagtgcgcg ctcgatgccg ccccgcccac tgcgataatc
      781 cacggcagat cggtgagcgt cggcgcgacc catccctgca gcgcgggaac gaagccgagc
      841 ggaagctgga ttgccgacat gtagaacagc accgcgagcg ccgaatcgtt ctcggacaag
      901 cgcttcgtgg cgatcatggt cgaggcgaag gcgaaggacc ctgcgagcat cacgagcgcg
      961 gccggctgca cgattccgaa tcccggcctc aggatgatga ggatgcccgc gagcccgagc
     1021 accagcatca cgatccggcc gcggttgagg cgttcgccca agacgagcat ggcgagcacg
     1081 gcggcccaca ccggcatggt gaattcgatc gcgaacacgg tcgcgagcgg cagcatcgcg
     1141 atcgcgtaca cccaggcgta ctgcccgccg aagtgcagca cgttgcgcac cacgtgcaga
     1201 ccgaagcgtt gcgtgcgcaa cacccctatt ccgaagcgcg gcaggacagc aagcaccaga
     1261 acgaaggaaa cgaggctccg aagaaacagg atctcgaagc ttcccatgct gcgaagcagc
     1321 tcgcgcaccg caacagccat gagcgagaac gagagcaacg ctccgaacat ccacagggca
     1381 ccgcgcagtg cggcgggtag tcgggacaaa ccggggacag accacggttt tgctgcttct
     1441 ctgttcaccg atcgattggc gataagttgg aaaccgtggt ctgtccccgg ttttcttcag
     1501 ccacgcgccg ggaaatcgct gcgctcgaga aggcgcgaga cggcgtagcc gaacacgagc
     1561 ccccagaacg gcgccccgat attgaagacc gggatgtcgg cgacggtcac gagaaaggtc
     1621 accagcgcgc cgagcgcgaa gcggtccctg aagctcaccg cgaacgcggt ctgcagcacg
     1681 cgcagcatcg cgaggcccgc gagcgccgcg acgaaggcct tgggcgcggc gagcataagg
     1741 cgcgtgaaga agggcgcgaa caggccgaag gcgaccgcaa gcaggccgac gaagatgccc
     1801 gcggtatagt gccttcgcct gtccccggag gacgatatga tcgcgttcac cgggcccgtg
     1861 aggcaggtgg agactgcgcc gacaaaggcc gtgacgatcg aacccgcccc gcacgcgatg
     1921 gtcacggcgt cgacgggcgg cgcgtggccg gcggagcgca ggacggtgat cccctgcccg
     1981 tcctgcacga cgagaacggt gatcgcgagc ggcacgacca gctccaccat cgcgcgccag
     2041 gaaaatgccg gagcgtagaa attcggatgc gcca
//
LOCUS       DASPZD010000193         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1156105, whole genome shotgun sequence.
ACCESSION   DASPZD010000193 DASPZD010000000
VERSION     DASPZD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156105"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /gene="pilB"
                     /locus_tag="VEM33_03745"
     CDS             <1..1720
                     /gene="pilB"
                     /locus_tag="VEM33_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HYS50969.1"
                     /translation="PNELHTATAAATSDKLGELLLRDGLITPDQLKKALLEQKNTGMR
                     LGYTLVKLGFIEETEISKMLARQYRMPAVDLSRFEVDPKIIKLMPPDIAVKHTVLPLK
                     REGRTLTVAIADPNNVTAIEDIKFVTRCDVFPVIAGEYTLRNAIERYYQQSDAQLQTL
                     LKDIEAAEDLEVVEDSVDEDVKAQDLADDAPVVKLINGLLTDAVKRGASDIHIEPFEH
                     ELRVRYRVDGALHEVMKPPVKMRAALTSRVKIMAQLNIAERRVPQDGRIKLKMGTKVI
                     DFRVSTLPVLFGEKIVLRILDKGNLTLDLKTFGFEPRSEADLLKAILNPYGMVLVTGP
                     TGSGKTTTLYSALSRINQIDVNIMTAEDPVEYNLLGINQVLVRNEVGMTFAAALKAFL
                     RQDPNIIMVGEIRDLETGSIAIKAALTGHLVLSTLHTNDAPSTITRMVDMGIEPFNVA
                     SAVNLIVAQRLVRRVCKECKTQHEYTPEEMHAFAIDKKEGPFFKGAGCDSCAGTGYRG
                     RQGLYEVMALTSPLRREILKGASTEELRDLAVKEGMLTLRMDGMLKVKKGVTTLEEVV
                     KETAAG"
     gene            1732..>2106
                     /locus_tag="VEM33_03750"
     CDS             1732..>2106
                     /locus_tag="VEM33_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HYS50970.1"
                     /translation="MPINLRALLEEMIEKDASDLHLVAGERPKLRVDGDIVSASSEEV
                     MTPKDTLSLAYSVLTENQKKRFETENELDFSFGIQNLARFRGNVFKQRGCVSVVIRQI
                     PFNVKTFNELGLPNVVAQLADRP"
BASE COUNT          443 a          655 c          675 g          333 t
ORIGIN      
        1 tcccaacgag ttgcatacgg ctaccgcagc agctaccagc gacaagctcg gcgaacttct
       61 gctccgcgac gggctgatca ccccggatca gctgaaaaag gctttgctgg agcagaagaa
      121 caccgggatg cgtctgggct acaccctggt gaagctgggc ttcatcgagg agaccgagat
      181 ctccaagatg ctggcccggc agtaccgcat gccggcagtc gacctgtcgc ggttcgaggt
      241 cgaccccaag atcatcaagc tcatgccgcc ggacatcgcc gtgaagcaca cggtgctgcc
      301 gctcaagcgg gaggggcgga cgctcacggt cgccatcgcg gaccccaaca acgtcaccgc
      361 gatcgaggac atcaagttcg tcacgcggtg cgacgtgttc ccggtgatcg ccggcgagta
      421 cacgctccgc aacgcgatcg agcgctacta ccagcagtcg gacgcccaac tccagacgct
      481 gctcaaggat atcgaggccg ccgaagacct cgaggtggtc gaagactcgg tcgatgagga
      541 cgtcaaagcg caggacctgg cggacgacgc gccggtcgtg aagctgatca acggcctcct
      601 gaccgacgcc gtgaagcggg gggcgtcgga catccatatc gagccgttcg agcatgagct
      661 gcgggtgcgc taccgcgtgg acggcgctct gcacgaggtg atgaagccgc cggtcaagat
      721 gcgagcggcg ctcacttcgc gcgtgaagat catggcgcaa ttgaacatcg ccgagcggcg
      781 cgtgccgcag gacgggcgca tcaagctcaa gatggggacc aaggtgatcg acttccgcgt
      841 gtcgaccctc ccggtgctgt tcggcgaaaa gatcgtgctg cggattctcg acaagggcaa
      901 cctgacgctc gacctgaaga cgttcggctt cgagccgagg tcggaggccg atctcctgaa
      961 ggcgatcctc aacccctacg ggatggtgct ggtgacgggg cccacaggct ccggcaagac
     1021 gaccacgcta tactcggcgc tgtcgcggat caaccagatc gacgtgaaca tcatgaccgc
     1081 cgaggatccg gtcgagtaca acctgctcgg catcaatcag gtgctggtgc ggaacgaggt
     1141 ggggatgacg ttcgcggcgg cgctgaaggc gttcctgcgc caggacccga acatcattat
     1201 ggtcggcgag atccgcgatc tcgagacggg ctcgatcgcg atcaaggccg cgctcacggg
     1261 ccacttggtg ctctcgacgc tgcacaccaa cgacgcgccc tcgaccatca cgcgtatggt
     1321 ggacatgggc atcgagccgt tcaacgtcgc gagcgccgtc aacttgatcg tcgcgcagcg
     1381 cctggtgcgc cgcgtctgca aagagtgcaa gactcagcac gaatacacgc ccgaagagat
     1441 gcacgccttc gccatcgata agaaggaagg cccgttcttc aagggcgcgg gatgtgattc
     1501 ctgcgcgggc accggttacc gcggccgcca gggcctctac gaggtgatgg cgctgacgag
     1561 cccgctgcgc cgcgagatcc tcaagggcgc ctcgacggag gagctgcgcg acctggccgt
     1621 gaaggaaggt atgttgacgc tccgcatgga cggaatgctg aaggtcaaga agggcgtgac
     1681 tacgctcgaa gaagtcgtca aagaaaccgc agccggataa ggagaggaag catgccaatc
     1741 aacctccggg cattgctcga ggagatgatc gaaaaggacg cttccgacct ccacctcgtg
     1801 gcgggcgagc gccccaagct ccgcgtggat ggcgatatcg tgagcgcctc cagcgaagaa
     1861 gtcatgacgc ccaaagacac cctgtcgctc gcgtactcgg tgctcaccga gaatcagaag
     1921 aaacgcttcg agaccgaaaa cgagctcgac ttctcgttcg gcattcagaa cctggcgcgt
     1981 ttccgcggca acgtgttcaa gcagcgcggc tgcgtgtcgg tcgtcatccg gcagattccg
     2041 ttcaacgtga agacgttcaa cgagctcggc ctgcccaacg tcgtcgcgca gctcgccgac
     2101 cggccc
//
LOCUS       DASPZD010000194         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1228725, whole genome shotgun sequence.
ACCESSION   DASPZD010000194 DASPZD010000000
VERSION     DASPZD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228725"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1983
                     /locus_tag="VEM33_03755"
     CDS             109..1983
                     /locus_tag="VEM33_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HYS50971.1"
                     /translation="MNQLKPGYRAALAFAHDVLASAACWVLAFWLRFNLELPPEEFLP
                     ALTAAVTAAVPLHALIFWSLGLYRGSWRYASLPDLKRIGVACLIGALAVPALLAFFRA
                     DVNVPRSTFILAPLLLAAIMSGSRIAYRAWRERSLYGHVHLTGEPVLVIGAGDVTVNL
                     LREMQRSSQWRAVGILDDETAWHGQVLLGVKVLGGLGEVGRWARQLESRHAIISLPGS
                     SAGVRRRAAEVAAAAGLTVLTVPSYDDLLSGKVTVSQIRRIELEDLLGRDQVVLDDGG
                     LHRLLTGRVVMVTGAGGSIGSELARQVAKFEPERLVLFELNEYALYCIEQEFLSSKKR
                     PDIAPIIGDVRNPARVQAVFSRFKPAVVFHAAAYKHVPLMEIDNTWEAAQNNVLGTLR
                     LARAARDHGVEKFVLISTDKAVNPTNVMGATKRLSEEVCQALQLPGRTRFVVVRFGNV
                     LGSTGSVIPKFREQIARGGPLTVTHPEVTRYFMSIPEAAQLVLQAGLMGNGGEIFVLE
                     MGEPIKIVDLARDMIRLSGLSEEDIRIEFTGLRPGEKLYEELLSDRERTVPTPHPKLR
                     IARAGDAPGETWLAEIETWLAQPRSPADEEVKRELARRVPGYASAAAGGAVRDIQEMG
                     "
     gene            complement(1968..>2312)
                     /locus_tag="VEM33_03760"
     CDS             complement(1968..>2312)
                     /locus_tag="VEM33_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYS50972.1"
                     /translation="HPGDDDKARAVTENCSRLPLLPCPTDELPRLIAALSLCDCVVCT
                     DGGAMHLAAALGKPILCFFGDSPPERWRPWKAPHELLQPASRDLADLTVEEALAGFER
                     LWRARPLAHPIS"
BASE COUNT          376 a          772 c          772 g          392 t
ORIGIN      
        1 gcgagccgct ccccctgcag ctgtgcgccc tcgtcgggct ggccgcgctg catgcaacgc
       61 tggcgctgtg ggtggaccat gcgtgccggc accgcgagag caaatccatt gaatcagctt
      121 aagcccggtt accgagccgc gcttgctttt gcgcacgacg tgctcgcttc cgccgcttgc
      181 tgggtgctcg ctttctggct gcgcttcaac ctggaactcc cccccgagga gtttctcccg
      241 gcgcttaccg cggcggtcac ggcggccgtt cctctgcatg ccttgatatt ctggtcgctc
      301 ggcctgtacc ggggcagctg gcgctatgcg agcttgcccg acctgaaacg cataggggtc
      361 gcctgcctga tcggagcgct cgcggtgccc gccctgcttg cgttctttcg cgccgatgtc
      421 aacgtgccgc gctcgacgtt tatcctcgcg cctctgctgc tcgcagcgat catgtcgggc
      481 agccgcatcg cctatcgcgc ttggagagag cgctccctgt acggtcacgt ccacctcacc
      541 ggcgaaccgg tgctcgtgat cggcgcgggc gacgtcaccg tcaatctgct gcgggagatg
      601 caacgctcca gtcagtggcg cgcggtcggc atactcgacg acgagacggc ctggcacgga
      661 caggtccttc tgggcgtgaa ggtgctcggg gggctcggag aagtcggccg ctgggcgcgg
      721 cagctggagt cccgccacgc gatcatctcg ctgccgggtt cgtccgcggg ggtgcgcagg
      781 cgcgcggcgg aagtcgccgc cgccgcgggg ctgacggtcc tcaccgtgcc ttcctacgac
      841 gacctgctct cgggcaaggt cacggtatcc cagatccgtc gcatcgagct ggaggatctg
      901 ctcggccgcg accaggtggt cctggacgac ggcggcctgc atcggctgct cacgggcagg
      961 gtcgtgatgg tcacgggggc cggcggctcg ataggctccg agctcgcccg ccaggtcgcc
     1021 aaattcgagc cggagcgtct cgtactgttc gagctgaacg agtacgcctt gtactgcatc
     1081 gagcaggaat tcctgtcgtc aaaaaaaagg ccggacattg cgcccatcat cggcgacgtg
     1141 cgcaatcccg cgcgcgtgca ggcggttttc tccaggttca aaccggcggt cgtgttccac
     1201 gccgctgctt acaagcacgt gccgctcatg gagatcgaca atacctggga agcggcgcag
     1261 aacaacgttc tcgggacgct gcgcctggcg cgcgccgcgc gcgatcacgg cgtggagaaa
     1321 ttcgttctca tctcgaccga caaggcggtg aacccgacca acgtcatggg agccaccaag
     1381 cgcctatcgg aggaagtgtg ccaggctctg caactccccg gccgcacacg cttcgtagtg
     1441 gtccgtttcg gcaacgtgct cggcagcacg ggcagcgtca tccccaagtt ccgcgagcag
     1501 atcgcacgcg gcggacccct gaccgtgaca cacccggaag tcactcgcta cttcatgtcg
     1561 attcccgaag ccgcacagct ggtgctgcag gcgggcctca tgggaaacgg cggtgagatc
     1621 ttcgttctgg aaatgggcga gccgatcaag atcgtcgacc tcgcgcgcga catgatccgc
     1681 ctttccgggt tgagcgagga ggacatcagg atcgagttca ccggcctgcg gcccggcgaa
     1741 aaactctacg aagaactgct ctcggaccgc gagcgcacgg tgcccacgcc gcaccccaag
     1801 ctgcgcatcg ccagagcggg cgatgcgccc ggtgaaacct ggcttgccga gatcgaaacc
     1861 tggctcgcgc agccgcgctc gcccgccgat gaagaggtca agcgggaact tgcgaggaga
     1921 gtgcccgggt acgcgtccgc cgccgcaggg ggggctgtgc gcgacattca ggaaatgggg
     1981 tgagcgaggg gccgagcgcg ccacaggcgc tcgaatcccg caagcgcttc ctcgaccgtc
     2041 aggtcggcaa ggtcgcggct cgcaggctgc agaagctcgt gcggcgcttt ccaaggccgc
     2101 caccgttcgg gcggcgagtc accgaaaaag cacaggatcg gcttgccgag cgccgcggcg
     2161 aggtgcatcg ccccgccgtc ggtacacacc acgcagtcgc acagcgacag ggcggcgatc
     2221 agcctcggaa gttcgtccgt gggacaaggg agtaggggaa gccggctgca gttttcggtc
     2281 acggcccgcg ccttgtcgtc atcccccgga tg
//
LOCUS       DASPZD010000195         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_19137, whole genome shotgun sequence.
ACCESSION   DASPZD010000195 DASPZD010000000
VERSION     DASPZD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19137"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VEM33_03765"
     CDS             complement(<1..947)
                     /locus_tag="VEM33_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS50973.1"
                     /translation="MPHDKDQTHWHEPTGTRKAGFGEFKKQPTPYDAFMESEGIPVFR
                     GIGIRRVQDLPLAPWKRTGGRGHYIQLHGTETKWGCHVVEIPPAGALNPEKHMYEEIF
                     LVVEGRGTTEVWQEGDAKRHVFEWQKGSMFSIPMNAWHRLVNATPGGALLLAGTTAPN
                     VLNMINNVSAVFNNPFVFRDRFNGADDFFKPKDDIEPDPVRGLAMRRTNFIPDIVNCD
                     LPLDNRRSPGWRRVEPFMTDNCFYFWIGQHENGRYSKGHAHTSAAVLICVKGKGYTYT
                     WPERLGVTPWKDGHADQVKRLDYEPVGMVSAAPGGARWY"
     gene            1077..2123
                     /locus_tag="VEM33_03770"
     CDS             1077..2123
                     /locus_tag="VEM33_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS50974.1"
                     /translation="MRWKDSIILFQALALCAGVPVASAQTPEKNHSVYLYQGADRAQR
                     LAELARKEGTLVVYTSLATSESVPLTQAFEKKHGVKVQLWRSLSDQVVRRALNEAKAR
                     RHVVDVVETNAPELEALARERVLAQFFSPHIADIPSWGVPLHRMWISDRVDFFVVAFN
                     TAKVRREELPATYEGFLDPKWRGRIGLEATDQEWLAGLAKYWGEKRALDFFRGLIAMK
                     PDVRKGHVLLSEMVAAGEVPVSLTNYASNADSMKRRDKPIDWKPVEPVIGRPQAIGLA
                     ANAPHPNAALLFADFVLSPEGQRLFYSMGRYPSSRKAESVRVAFPYLMLDPIAMVDED
                     EKWLKLWNELVTVK"
     gene            2125..>2513
                     /locus_tag="VEM33_03775"
     CDS             2125..>2513
                     /locus_tag="VEM33_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HYS50975.1"
                     /translation="MTSPLPHDLAGLVLMVFLLGLRHGMDPDHLAAVDGLARHNARDR
                     PSIARWSGCFFSMGHGAVVTLIAGFVAATLHDGAAPQWLERLGAWISIVFLLALGIVN
                     VVAVLRADRVVRPAGFKVRWLGRVAETG"
BASE COUNT          412 a          786 c          839 g          476 t
ORIGIN      
        1 tggtaccagc gcgcgccgcc gggggccgcc gacaccatgc cgaccggctc gtagtcgagg
       61 cgcttcacct ggtcggcgtg gccgtccttc cacggcgtca cgcccagccg ctcgggccag
      121 gtgtaggtgt agcctttgcc cttgacacag atgagcacgg cggccgaggt atgcgcgtgt
      181 cccttcgagt agcggccgtt ctcgtgctgg ccgatccaga agtagaagca gttgtcggtc
      241 atgaacggtt cgacgcgccg ccatcccggg gagcgcctgt tgtccagggg aagatcgcaa
      301 ttcacgatat cgggaataaa gttcgtgcgc cgcatcgcga ggccgcgcac gggatcgggc
      361 tcgatatcgt ccttgggctt gaagaaatcg tccgcaccgt tgaaacggtc gcggaacacg
      421 aagggattgt tgaacaccgc gctcacgttg ttgatcatgt tgagcacgtt cggcgccgtg
      481 gtgcccgcga gcagcagcgc gccgcccggg gtggcgttca ccaggcgatg ccaggcgttc
      541 atcgggatcg agaacatcga gcccttctgc cactcgaaga cgtgtcgctt tgcgtcgccc
      601 tcctgccaga cttcggtggt gccgcggccc tcgacgacga gaaagatctc ctcgtacatg
      661 tgcttctcgg gattgagcgc gccggcggga gggatctcca cgacatggca gccccacttg
      721 gtctcggtgc cgtgaagctg gatatagtgg ccgcgcccgc ctgtgcgctt ccagggcgcg
      781 aggggcaggt cctgcactct gcgtatcccg atgccgcgga acacggggat gccctcggat
      841 tccatgaagg cgtcgtacgg agtcggctgc ttcttgaact cgccgaatcc ggccttgcgc
      901 gtgccggtcg gttcgtgcca gtgggtttgg tctttgtcgt gcggcatcga cagctcttct
      961 cgttctgata tacagaacac ggttccaact tttttgcaaa gcgctctcat tcgaccatat
     1021 aatccccgcg agcgtcaatc agcgccgcgg gcgaaaaacg ccggaggagg gaagcgatgc
     1081 ggtggaagga ctcgatcatc ctgtttcagg cgctcgctct gtgcgcgggc gttccggtcg
     1141 cgagcgcgca aaccccagag aaaaatcact ccgtatacct ctaccagggt gccgaccgcg
     1201 cgcagcgcct cgccgagctg gcgcggaagg aggggacgct cgtcgtgtac acgtcgctcg
     1261 ccacctccga gtcggtgccg ctcacccagg cgttcgaaaa gaagcacggt gtcaaggtcc
     1321 agttgtggcg ctcgctcagc gaccaggtgg tcaggcgcgc cttgaacgaa gccaaggcgc
     1381 gccgtcatgt cgtggacgtc gtcgagacca acgctccgga gctggaggca ctggcgcgcg
     1441 agcgggtcct ggctcagttc ttcagccctc atatcgctga cattccttcc tggggagtgc
     1501 ctctgcaccg gatgtggatc tcggaccggg tcgatttttt cgtggtggcg ttcaatacgg
     1561 cgaaggtgcg ccgcgaggag cttcccgcta cttacgaggg cttcctcgac cccaagtgga
     1621 gagggcggat cgggctcgaa gcgaccgatc aggagtggct cgccgggctc gccaagtact
     1681 ggggcgagaa gcgcgccctc gatttcttcc gagggctgat cgcgatgaaa cccgatgtgc
     1741 gcaaggggca cgtgctcctg tccgagatgg tcgcagccgg tgaggtgccc gtcagcctga
     1801 ccaattacgc atccaacgcc gattcgatga agcgccgcga caagcccatc gactggaagc
     1861 cggtcgagcc ggtgatcggc aggccgcagg cgatcgggct cgcggcgaac gcgccccacc
     1921 cgaacgccgc gctgctcttc gccgatttcg tgctgtcccc ggaaggtcag cggcttttct
     1981 attccatggg gcggtatccg tcgagccgaa aggcggaaag cgttcgggtg gcgtttccct
     2041 acctgatgct cgacccgatc gctatggtcg acgaagacga aaagtggctc aagctgtgga
     2101 acgagctcgt cacggtgaaa taggatgact tcgcccctgc cgcacgacct cgccggcctc
     2161 gtgctgatgg tgttcctcct gggccttcgg cacggaatgg atccggacca cctggccgcg
     2221 gtcgacgggc tcgctcgcca caacgcccgg gaccggccga gcattgcgcg ctggtccgga
     2281 tgcttcttct ccatgggcca tggcgccgtc gtcacgctga tcgccggttt cgtggcggcc
     2341 acgctgcacg acggcgcagc gccgcaatgg ctggagcggc tcggcgcctg gatttccatc
     2401 gtgtttctgc tcgcgctggg aatcgtgaat gtcgtggcgg tcctccgcgc ggaccgcgtc
     2461 gtgcgtccgg cggggttcaa ggtgcgatgg ctgggacggg tcgcagaaac cgg
//
LOCUS       DASPZD010000196         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1167441, whole genome shotgun sequence.
ACCESSION   DASPZD010000196 DASPZD010000000
VERSION     DASPZD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167441"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VEM33_03780"
     CDS             <1..865
                     /locus_tag="VEM33_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYS50976.1"
                     /translation="IEVIRRLRRIFGYEDVPIVCITVVNDREVRCRALEAGATDFLIR
                     PIDRLECGARCRNLLSLRRHEILKKNRTRILEQKIDEATRELRRREVETLFRLAKAAE
                     QRDKVTGMHLVRMAKYSSLIAKGIGLSSAEQDAIELAAPMHDIGKIGIPDRILQNPGR
                     LDGEDLRVMQSHARIGYDMLKDSSSGYLQMAAVIALGHHEKYDGSGYPTGLRGEHIPL
                     EARIVAIADVYDALTSVRPYKPAWPVEEALVYMVEHAGRHFDPQLVEILSRNRDEVLS
                     IGERFADRLAQ"
     gene            884..>2689
                     /locus_tag="VEM33_03785"
     CDS             884..>2689
                     /locus_tag="VEM33_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS50977.1"
                     /translation="MALKSMLAGVRARFRGRPDTEHEQAIVRLLIAAILFLYLLPQAF
                     GGSESNLPLFGAMICYFTLCATVFAWIYLFPAASPARRVFGAILDVVSTSAFMYYLGE
                     YGAVLYIAYLVVTFGHGFRYGKPYLYNSLVLSVVGFALVLTFSDYWIQNRVLGIGLMV
                     GMILLSLFQGKLVTRLFDSLRREEAANQAKRRFLSTVSHEMRTPLNAIVGMNDLLRDT
                     PLDTEQAEMVKAMHEASRSMLKLIEDVLDISKIEAGKVNIEETDFDLHSLINGTAAVL
                     APQAEIRGLLFRTHVMPEVPHALRGDPYHLRQVLYNLIGNGVKFTQSGSVVLTVSSLG
                     ESEHAVRLKFSIEDTGIGIAPQAQERIFESFVQADDSTTRRYGGTGLGTTISKQLVEM
                     MGGQIGLNSTLGKGSTFWFDLTFKKQMTGQGAEASLRLNDIRIMLVGFSDAELQALEQ
                     DLATWGARTGRAAGVEAGAARLAEAQSLGHPYHLVLLREDTTVAPGRNLGRLRNSRSQ
                     KAPPALLCTPGVSQIRSAEALAGGFVAVLEMPFKKRLLFNAIHSATAVQGSQEGVISL
                     SDYYAARDANRRRYQILVAEDNAVNQRVLVGILERA"
BASE COUNT          504 a          860 c          840 g          485 t
ORIGIN      
        1 aatcgaggtc atccggcgct tgcgccgcat cttcggctac gaggacgttc cgatcgtctg
       61 tatcacggtg gtcaacgatc gcgaagtccg ctgccgcgcc ctggaagccg gggctaccga
      121 cttcctgatt cgcccgatcg accggctcga atgcggcgcg cgctgccgca atctcctgag
      181 cctgcgccgt catgaaatcc tcaagaagaa ccgaacccgg attctcgagc agaagatcga
      241 cgaggccacc cgcgaattgc ggcggcgcga ggtcgagacg ctgtttcggc tggcaaaggc
      301 cgcagagcag cgcgacaagg tgaccggaat gcacctggtg cggatggcca agtactcctc
      361 gctgatcgcc aagggcatcg ggctgtcctc ggccgagcag gacgcgatcg agctggcggc
      421 accgatgcac gatatcggaa aaatcggcat accggaccga atcctgcaga acccaggaag
      481 gctggacggc gaggacctgc gggtcatgca gtcccacgcg cgcatcggct acgacatgct
      541 gaaggacagc tcttccggat atttgcaaat ggccgccgtg atcgcgctcg gccatcacga
      601 gaagtacgac ggcagcggct acccgaccgg cttgagaggc gagcatattc cgcttgaagc
      661 gcggatcgtc gccatcgcgg atgtctacga cgcgctcacc tcggttcgac cctacaagcc
      721 ggcctggcca gtggaagaag cgctcgtcta catggtggag cacgccggcc ggcattttga
      781 tccgcaactt gtcgaaattc tgtcgcggaa ccgagacgaa gtcctttcca tcggcgaacg
      841 gtttgccgat cggctggcgc agtagacccc gggacccagc cggatggcgc tgaaatcgat
      901 gcttgcaggg gttcgtgctc gctttcgcgg gcgccccgac accgagcacg agcaggcgat
      961 cgttcggctg ttgatcgcgg caatactgtt cctgtatctc ctcccgcagg cctttggcgg
     1021 cagcgaatcc aatctgccgc tgttcggcgc catgatctgc tacttcacgc tgtgcgccac
     1081 ggtattcgcg tggatttacc tcttcccggc ggcatcgcct gcgcgccgtg ttttcggcgc
     1141 aattctggac gtcgtctcga ccagcgcgtt catgtattac ctgggcgaat acggcgcggt
     1201 tctgtacatc gcttacctgg tggtcacctt cggccacggt ttccgctacg gcaagcccta
     1261 tctgtacaac tcgctggtac tcagcgtcgt gggtttcgcc ctggtcctga cgttcagcga
     1321 ttactggata caaaaccgcg tgctcggcat cggcctgatg gtgggcatga tcctgctgtc
     1381 gctcttccaa ggcaagctcg tgacccgcct gttcgattcg ctgcgtcgcg aggaagccgc
     1441 gaatcaggcg aagcggcgct tcctctccac ggtcagccac gagatgcgca ccccgctcaa
     1501 cgccatcgtc gggatgaacg acctgctgcg cgacacgccc ttggacacgg aacaggccga
     1561 aatggtgaag gcgatgcacg aggcctcgcg atcgatgctc aagctgatcg aggacgtgct
     1621 ggatatttcc aagatcgaag ccggtaaggt caacatcgaa gaaacggatt tcgatctgca
     1681 cagcctcatc aacgggacgg cggccgtgct tgcaccgcag gccgagatcc gcgggctgct
     1741 gttcaggacc cacgtgatgc ccgaagtgcc gcacgcgctg cgcggcgatc cctatcatct
     1801 gcgccaggtg ctctacaacc tcatcggaaa cggcgtcaag ttcacccagt ccggcagcgt
     1861 cgtcctgacc gtttcgagct tgggcgagag cgagcatgcg gtgaggctga aattttccat
     1921 tgaggacacg gggataggca tcgcgccgca agcccaggaa cgcattttcg agagtttcgt
     1981 tcaagccgac gactcgacca cgcgccgcta cggcggcacc gggctcggca ccacgatctc
     2041 gaagcagctg gtggagatga tgggcgggca gatcggcctg aacagcactc tcgggaaggg
     2101 cagcacattc tggttcgacc tgacctttaa gaagcagatg acgggccagg gggccgaagc
     2161 ttcgttgcgg ctcaacgata tccggatcat gctggtcggt ttctccgacg cagaactgca
     2221 ggcgctcgag caggacctcg ccacttgggg ggcgcgcacc gggcgcgcgg caggcgtcga
     2281 ggcgggcgcc gcgcgcctag ccgaagcgca gtcactcggg catccatacc acctggtgct
     2341 gctgcgcgaa gacacgacgg tcgccccggg ccggaatctg gggcgccttc gcaacagccg
     2401 cagccagaag gcgcctcccg cgctgctgtg cacccccggc gtatcgcaga ttcgcagcgc
     2461 cgaagcgctc gcgggcggtt tcgttgcggt gctggagatg ccgttcaaga agcggctgct
     2521 gttcaacgcc attcattccg cgactgcggt ccaaggcagc caggaaggcg tcatctccct
     2581 cagcgattac tacgccgccc gcgacgccaa ccggcgccgc taccagatcc ttgtggccga
     2641 agacaacgcc gtcaatcagc gcgtcctcgt cgggatactc gagcgcgcc
//
LOCUS       DASPZD010000197         4877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_981633, whole genome shotgun sequence.
ACCESSION   DASPZD010000197 DASPZD010000000
VERSION     DASPZD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981633"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VEM33_03790"
     CDS             complement(<1..518)
                     /locus_tag="VEM33_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HYS50978.1"
                     /translation="MVRPLPVLNVLSVLIVAFSIAMLVPLGVSFMYDDAARRAYYEAV
                     LITFVSGAALWVVTRSAKAELQTRDGFLLVALVWTVLPAFATLPLLIYMPGLSFTDAY
                     FETVAALTTTGSTVLSGLDKLPPSINLWRCELQWLGGMGIIVLAVAILPLLGIGGSQI
                     MRPETPGPMKDT"
     gene            complement(536..1909)
                     /gene="trkA"
                     /locus_tag="VEM33_03795"
     CDS             complement(536..1909)
                     /gene="trkA"
                     /locus_tag="VEM33_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HYS50979.1"
                     /translation="MKIVILGAGQVGSSVAENLASEANDITVVDIDAARLRNLQDRLD
                     LRTVVGSAAHPSVLVEAGIEDCDMMIAVTQSDETNLVACKLAQQLFNVPTRIVRLRAS
                     DYLSNERVLGPDCFDVDLAICPEQILTDYIVKLIEFPEALQVLEFAHGKVSLVAVRAF
                     QGGPLVGHPLKDLRKHIPSVDTRIAAIFRGDSPILPEGDTVVQAGDEVFCLAATEDIR
                     QVMHELRRMDQAVRRVMIAGGGNIGLRLARAIERDYAIKIIEYDKRRCEVLSTGLDRA
                     LVLQGDVTDEGLLEAENVSDMDLFVAVTNDDENNIMSCLLAKRMGARRVVALINRRSY
                     VDLLQAGQIDIAISPAQATIGTLLAHVRRGHITRVHSLRRGVAEALEAVVHGDRDSCR
                     CVGRRIEEIDLPKGAAIAAIVRGDKVIMAHHDTVVLAEDHLIVFVTDKKLLPKVEKLF
                     EVSAGFV"
     gene            complement(1938..2117)
                     /locus_tag="VEM33_03800"
                     /pseudo
     CDS             complement(1938..2117)
                     /locus_tag="VEM33_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2388..2804)
                     /locus_tag="VEM33_03805"
                     /pseudo
     CDS             complement(2388..2804)
                     /locus_tag="VEM33_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2838..3182)
                     /locus_tag="VEM33_03810"
                     /pseudo
     CDS             complement(2838..3182)
                     /locus_tag="VEM33_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3189..>4877)
                     /locus_tag="VEM33_03815"
     CDS             complement(3189..>4877)
                     /locus_tag="VEM33_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS50980.1"
                     /translation="DLSETPRRDQPAALARLREQAYVEEAWLITEGGQVLARASREPG
                     KLVAPSPSAQALRDARQARGYAVVEPVGDKGLLLRVIVSLETSRRADESRLLQLTHWV
                     PQTLAEQGESVQSVYRAYKELSLSRQGLKEIYILTLTLTLLLALLSAIALAFLLSRRL
                     SNPLAVLAESTQAVARGDFSRRARVTSRDELGILTQSFNSMTQQLGEAQDAASLNQAQ
                     LETAKAYLESILANLSAGVLVFDHGLTLRIANSGAGSILHENVAALIGLKLENWRTLP
                     EFARMLQAEFEQHRGTVWQQQIEMRERGAVILVRGSPLPETGEGGYVVVFDDVTQLIA
                     AQRATAWGEVARRLAHEIKNPLTPIQLAAERLQAKLGDKLSPEAARSLDHATETIVAQ
                     VTAMKNMVDDFRDYARTPPPQLGGLDLNRLVTEVLALYEQSGTRIQANLQRSIPLVRG
                     DPDRLRQVIHNLLQNAQDALFGSTDPKIEVSTELSGGQVWLRISDNGCGFPDAIINGA
                     FEPYVTTKPKGTGLGLAIVKRIIEEHHGTVRIENHIGKEGGRGAAVRISLPLAA"
BASE COUNT          867 a         1558 c         1539 g          913 t
ORIGIN      
        1 ttggtgtcct tcatcggccc cggggtctct ggcctcatga tctggctccc gccgatgccg
       61 aggagcggca ggatcgccac cgcgaggacg atgatcccca ttccgcccag ccactgcagc
      121 tcgcagcgcc acaagttgat cgacggcggc aatttgtcca gccccgacag cacggtcgaa
      181 ccggtagtgg tgagtgctgc cacggtctcg aagtaggcgt ccgtgaaact gagccccggc
      241 atgtaaatca gcaggggcag ggtggcgaag gcggggagga ccgtccaaac gagcgccacc
      301 aggagaaacc cgtcccgggt ctgcaactcg gccttggcgg aacgggtgac cacccacaat
      361 gctgctccgc tcacgaaagt aatcagcaca gcctcgtaat aggcgcgccg cgccgcatcg
      421 tcgtacatga acgagacgcc gagcggcaca agcattgcga ttgaaaaggc gacgatcaag
      481 acgctcagca cgttgagaac gggaagcggc ctgaccatgg gtgccggcgt ccagctcaga
      541 cgaagccggc gctgacctcg aagagtttct ccaccttggg caggagtttc ttgtcggtga
      601 cgaagacgat caagtggtcc tcggcaagca ccacggtgtc gtgatgggcc atgatcacct
      661 tgtcgccgcg cacgatcgcg gcgattgcag cgcccttggg caggtcgatt tcctcgattc
      721 tccggccgac gcaccggcag gaatctcgat ccccgtgcac caccgcctcg agcgcttccg
      781 ccaccccgcg cctcaggcta tgcacccggg tgatgtggcc gcgccgcacg tgggcgagca
      841 gcgtgccgat ggttgcctgc gccggggaaa tcgcgatatc gatctggccg gcttggagca
      901 gatcgacgta ggagcgccgg ttgatcagcg cgaccacgcg gcgtgcgccc atgcgcttcg
      961 caagcaggca ggacatgatg ttgttctcgt cgtcgttggt taccgcgacg aagaggtcca
     1021 tatcggaaac gttttcggcc tccaggagcc cttcgtccgt gacgtcgccc tgcaacacga
     1081 gggcccggtc gaggccagtg gagagcacct cgcaacgcct cttgtcgtac tcgatgatct
     1141 tgatcgcgta gtcgcgctcg attgcacgcg cgaggcgcaa gccgatgttg ccgccccccg
     1201 cgatcatgac tcttctaacc gcttggtcca tgcgcctcag ttcgtgcatg acttggcgta
     1261 tatcttccgt ggcggcgaga caaaatacct cgtctccggc ctgcaccacc gtgtcgccct
     1321 cgggcagaat cgggctgtca ccgcgaaata tggcggcgat acgcgtgtcc acggacggaa
     1381 tgtgcttcct gagatccttc agaggatggc cgaccagggg accgccctgg aatgcacgca
     1441 ctgcgaccaa gctcaccttg ccgtgagcga actcgagcac ctgcagtgct tccggaaact
     1501 cgatgagctt gacgatgtag tcggtaagga tctgctccgg acagatcgcc agatccacgt
     1561 caaagcagtc cgggccgagc acgcgctcgt tggaaaggta gtcgctcgcg cgcaggcgga
     1621 cgattcgggt cgggacgttg aacagctgct gcgccagctt gcatgcgacc aggttggttt
     1681 cgtcgctttg cgttaccgca atcatcatgt cgcagtcctc gatgcccgcc tcgaccagca
     1741 ccgaggggtg cgcggcgctg ccgaccacgg tgcgcaagtc caggcggtcc tggaggttgc
     1801 gcagcctcgc ggcgtcgatg tccacgacgg taatgtcgtt ggcttcggat gccaggtttt
     1861 cggcgacaga tgagccgact tggccagcgc cgaggattac gattttcaag gcgggaatcc
     1921 gcgcgaaaaa cccggagtca gtcttctttt cgcccggcga gaatcccgag cgctttcaac
     1981 ttgcggtaca agtgcgtgcg ctcgagaccg cttcgctcgg cgacgcgcgc tatggatccg
     2041 ttcacctgcg cgagatggtg ctcgaagtag gttcgctcga aggcttcgcg agcctcgcgc
     2101 aggggaagat cgagcgacag gtccgtctcg gcggagcggc gcgcaaactg cggcagcacc
     2161 cgctccgcgt ccgcgacgcc gatctcctcc tccaggctgg tgagcgccag gttgcgcacc
     2221 gctgcggcga gatcttctag gttacccggc cagaggaaat accgcaggac gttgagggca
     2281 ccggtggaga aggttcgcgg cgggcaaaag cgcgtttcca ccagctgggc aagcatcagg
     2341 cttgcgatct cgggaatgtc ctccggatgc tcacgcagag ccggaagcga gatgacgatt
     2401 cccgcgagcg cttgcatcaa tgccgcgtcg aaaccctgcg attccaccag gtgtttcggc
     2461 tcctcggcgg taaaagacac cagctgcacg ttgcttcttc ccgcgcgcgc ggcgagaaac
     2521 gccaggtgct tctgctggtt ccgggacagc atcgcgagtt ccccgacgaa gaggacgccg
     2581 ctggaaacct gctgcagcaa atcctgagac gcctcgttga gtgcgtgcgc cgcatcgatc
     2641 cagggtgcgt tgggctgctg cagatagcgc gcgtagagct ccggcatcac gcctctttcg
     2701 ccgcgcagca gcacggctgt cccggcggca gagatcctcg ccaggcgctt cttcagctcg
     2761 gtcatgatca ccgaacggcc gaaattcacg atgctgagtg gcatcacggc gcgcgattcc
     2821 cccgcacgca gtgcacgctt cacggttgcg aggagcttct gcagcgcgat cggtttttcc
     2881 aggaaatcca ttgcgccgca gcgcgtcgcc tccaccgccg tgtcgatggt gccgtgaccc
     2941 gacatcatga tcaccggcat ggtgagctgg cccccggtgg cccattcctt caggagcgta
     3001 atgccgtccg cgtcgggcat ccaaatatcg agcaacacga ggtccggacg tctctgcgcg
     3061 cgcaacgagc gcgccgcggt ggcattctcc gcaagctgca ccgagtgtcc ttcgtccgag
     3121 aggatttcgg acaagagctc gcggatcccg acttcgtcgt cgacgactag tatctgcgcc
     3181 atggtcgctt aggcggcgag cgggagcgaa atccgcaccg ccgcgccccg acccccttcc
     3241 ttcccgatgt ggttttctat ccgaacagtg ccgtgatgct cctcgatgat ccgctttacg
     3301 atcgccagcc ccagtccagt tcccttgggc ttggtggtga cgtagggctc gaacgccccg
     3361 ttgatgatgg catcggggaa gccgcaaccg ttgtcgctga tccgcagcca cacttggcca
     3421 cccgaaagct cggtcgacac ttcgattttc gggtcggtgc ttccgaaaag agcatcctgc
     3481 gcgttttgca gcagattgtg gattacctgt cgcaggcggt cggggtcacc gcgcaccaat
     3541 ggaatgctcc tctggaggtt ggcctgtatc cgagttcctg actgttcgta caaagcgagt
     3601 acctcggtaa cgagccggtt caggtccagt ccgcctaatt gcggcggggg cgtgcgagca
     3661 tagtcgcgga aatcatccac catgttcttc atggcggtga cctgcgcgac gatggtttcc
     3721 gtggcgtggt cgagcgagcg cgccgcctcg ggcgagagtt tgtcgccgag tttcgcctgg
     3781 agtcgctccg ccgcgagctg gatcggcgtg agcggattct tgatctcgtg agcgagcctg
     3841 cgcgcgacct cgccccaggc ggtggcgcgc tgtgccgcga tcaattgcgt gacgtcgtcg
     3901 aataccacga cgtacccgcc ctccccggtc tcgggcagcg gcgacccgcg tacgagaatc
     3961 accgcgcctc gctcgcgcat ctcgatctgc tgctgccaga cggttcctct gtgttgttcg
     4021 aactccgctt gcagcatgcg agcaaactcg gggagcgtgc gccaattctc gagtttgagt
     4081 ccgatcaatg cggcaacgtt ctcgtgcagg atgctgcctg ccccgctgtt tgcaatccgg
     4141 agggtgaggc catgatcaaa aacgagcaca cccgccgaca gattggcgag aatgctctcc
     4201 aggtacgctt tggcggtttc gagctgcgct tgattgagcg acgcggcgtc ctgcgcctcg
     4261 cccagctgtt gagtcatgct gttgaacgac tgcgtgagga tgcccagctc atcgcggctc
     4321 gtgacccgag cgcggcgcga aaaatcgccg cgcgccaccg cctgggtgct ctcggcgagc
     4381 acggcgagcg ggttcgacag gcgccgcgac agcaggaacg cgagtgcaat agcggaaagc
     4441 aaagcgagca acagcgtcaa ggtcagcgtg agaatgtaga tctccttcag cccctggcga
     4501 gacagcgaca gctccttgta tgcacgatac acgctttgca ccgattcgcc ctgttcggcg
     4561 agcgtctgcg gcacccagtg cgtgagttgc agcaagcgcg attcgtctgc gcgcctggaa
     4621 gtctcaaggg acacgatcac tcgcagcaag agtcccttgt cgcccaccgg ttcgaccacg
     4681 gcataaccgc gcgcctggcg tgcatcgcgc aacgcctgtg cacttgggct gggcgccacg
     4741 agtttcccgg gttcccggct cgccctcgcg agcacctgtc caccttcggt tatgagccac
     4801 gcttcctcga cgtaggcctg ctcgcgcaaa cgagcgagcg ccgccggttg gtcgcgcctc
     4861 ggggtctcgg agagatc
//
LOCUS       DASPZD010000198         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1172377, whole genome shotgun sequence.
ACCESSION   DASPZD010000198 DASPZD010000000
VERSION     DASPZD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172377"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1015)
                     /locus_tag="VEM33_03820"
     CDS             complement(56..1015)
                     /locus_tag="VEM33_03820"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HYS50981.1"
                     /translation="MSMADRDGFIWYDGKLVPWRSATTHVLTHSLHYGLAVFEGLRAY
                     KTVDGTAIFRLEDHTERLFNSAHIYMMKIPYGRETLIEAQKEVVRANQHDSCYIRPIA
                     FYGSEKMGVSPIGATVHVAIAAWPWGAYLGPEAIEKGIRVKTSSFARHHINVTMARSK
                     MAGTYPNSILANLEATQHGYDEGLLLDVDGFVAEGSGENIFIVKDGKLHEPELTSALS
                     GITRASVITLANELGYEVVSRRLTRDDIYIADEAFFTGTAAEVTPIRELDGRTIGAGT
                     RGPVTTKIQKLFFDVIAGKVAKHKDWLSPVNEKASSRVSKENA"
     gene            1248..>2713
                     /locus_tag="VEM33_03825"
     CDS             1248..>2713
                     /locus_tag="VEM33_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50982.1"
                     /translation="MDQPLQRLEQLEQAIEETIAEAIPPKSRWRVAARIAGWMLLVAY
                     FLFAAALLALRYWILPKVAEYRGDIEQYASKALGQRVTIGAIEADWQGLRPELLLANV
                     TVFDHDGRAALSLPAVEATLAWISALVGSPRFYSLVFDRPSLEIRRDEAGRFYVAGIE
                     LHAQQAGDAGIAQWVLSQREIVIRDASVSWDDEQRGAPLLELPAFNFVLRNGFLGHRF
                     AFKAKPSAELASTLDVRGDLDSLDAADPGACVGKIYAELEYTDLVAWRRWVDYPVDIR
                     SGQGGVRLWLNLRGKASTELTVDVALSGVAGRVAKDTPLLELDYLRGRLGGSQRDGKE
                     FEVFGRKLTLGTATGIVVPPADFRVHWAPSGGDLDADAIELAPLARLAEYLPFPRATR
                     ARLAATDPRGSVRDLKAAWTGDVEDPQHYSVRGSFAKLAARAHDRIPGFAGLTGLFEG
                     SEKGGSLVLDSERVAIELPGIVAESPLQFDSLAAHIGW"
BASE COUNT          434 a          871 c          914 g          494 t
ORIGIN      
        1 ggaagatccg cctcggtgac ctccacgacg cggcttgccg gttcgctggc gcccatcagg
       61 cgttttcttt ggacaccctg gacgaggcct tctcgttgac cggcgagagc cagtccttgt
      121 gcttggcgac cttgcccgcg atcacgtcga agaacagttt ctggatcttg gtcgtgaccg
      181 gtccgcgcgt gcccgcgcct atcgtgcgcc cgtcgagctc gcggatgggc gtcacttcgg
      241 ccgcggtgcc ggtgaagaaa gcctcgtcgg caatgtagat atcgtcgcgc gtaaggcgcc
      301 gcgaaacgac ttcgtagccc agctcgttcg ccagcgtaat caccgaggcg cgcgtgatcc
      361 cgctcaaggc ggaagtcagc tccggctcgt ggagctttcc gtccttgacg atgaagatat
      421 tctcgcccga cccttccgca acgaagccgt cgacgtcgag gagcaagccc tcgtcgtagc
      481 cgtgctgggt cgcctccagg ttcgcgagga tcgagttcgg ataggtcccg gccatcttgg
      541 agcgcgccat ggtcacgttg atgtgatggc gggcgaagga agaagtcttg acgcgtatgc
      601 ccttctcgat cgcctccggc ccgaggtaag cgccccaggg ccacgccgcg atggcgacgt
      661 gcaccgtcgc gccgatcggg gacacgccca ttttctccga gccgtagaac gcgatcgggc
      721 ggatgtagca ggagtcgtgc tggttggcgc gcaccacttc cttctgcgcc tcgatgagcg
      781 tctcccggcc gtaggggatt ttcatcatgt agatgtgcgc cgagttgaac aggcgctcgg
      841 tgtggtcttc gagcctgaag atcgccgtcc cgtcgacggt cttgtaggcg cgcaggccct
      901 cgaatacggc gaggccgtag tgcagcgaat gggtgagcac gtgggtggtc gcggaacgcc
      961 agggcacgag cttgccgtcg taccagatga atccgtcgcg atcggccatc gacatgggaa
     1021 tctccgggaa gttttccgaa aggagcttat tctagcggaa acgcgcgaaa atcgaggagc
     1081 ttcagcgagt tgtcagcgca ttcttctccc ggcgacaagg gcttgcaggt aaaatgtcgc
     1141 tcatcgcagg tgcttgtcgt tgcgaggcga tggcctcgcg ttcatccttg gtcgccgatc
     1201 acggctttgc cgtggggccg aacttccacg ccgaaaagcg cactgacttg gaccagcccc
     1261 ttcagagact agagcaactc gagcaggcga tcgaggaaac gatagccgag gcgattccgc
     1321 cgaaaagccg gtggcgcgtc gccgcgcgca tcgccggctg gatgctgctc gtcgcctact
     1381 tcctgttcgc cgcggccctc ctcgcccttc gctactggat cctcccgaag gtcgcggagt
     1441 accggggcga catcgaacaa tacgcgtcga aggcgctggg ccagcgcgtc acgatcggcg
     1501 ccatcgaggc ggactggcaa gggctgcgcc cggagcttct tctcgccaac gtgaccgtct
     1561 tcgatcacga cggccgcgcc gcgctgtctc tgcccgcggt cgaggccacg ctcgcctgga
     1621 tctccgccct ggtcggatcg ccgcggttct attcgctcgt cttcgaccgc cccagcctcg
     1681 agatcaggcg cgacgaggcg ggcagattct acgtcgcggg tatcgagttg cacgcccagc
     1741 aggccggtga tgccggaatc gcgcaatggg tgttatcgca gcgcgagatc gtcatccgcg
     1801 acgcgagcgt cagctgggac gacgagcagc gcggggcgcc gttgctcgag ttgccggcgt
     1861 tcaatttcgt gctgcgcaac ggcttcctgg ggcaccggtt cgccttcaag gccaagccgt
     1921 ccgccgaact cgcctcaacg ctcgatgtgc gcggtgatct ggacagtctg gatgccgccg
     1981 atccgggagc gtgtgtcggg aagatctacg ccgagctcga atacaccgat ctcgtcgcgt
     2041 ggcggcgctg ggtcgactat ccggtcgaca tacgctcggg ccagggcggg gtgcgcctgt
     2101 ggctgaatct ccgcggcaag gcctccaccg agctcacggt cgacgtcgcg ctttccgggg
     2161 ttgcgggccg cgtcgccaag gacactccgc tcctggagct cgattacctg cgcgggcggc
     2221 tgggcgggag ccagcgcgac gggaaggagt tcgaagtctt cggccgcaag ctcacgctcg
     2281 gaaccgcgac cgggatcgtc gtcccgcccg ccgattttcg ggtgcattgg gcgccttccg
     2341 ggggggacct ggacgccgat gcgatcgagc tcgcgccgct cgcaagactc gccgagtacc
     2401 tgccgtttcc gcgggccacc cgtgcgcgcc tggctgcaac cgatccgcgc ggcagcgtgc
     2461 gcgacttgaa agccgcctgg acgggagacg tggaggatcc gcagcactac agcgtgcggg
     2521 gaagcttcgc gaagctcgcc gcgcgcgccc acgacaggat tcccggattc gcggggctca
     2581 ccggactgtt cgaggggagc gagaagggcg gcagcctcgt gctcgactcg gagcgggtcg
     2641 cgatcgagct gcccggaatc gtcgccgaga gcccattgca gttcgattcg ctcgcggcgc
     2701 atatcggctg gaa
//
LOCUS       DASPZD010000199         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_147315, whole genome shotgun sequence.
ACCESSION   DASPZD010000199 DASPZD010000000
VERSION     DASPZD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147315"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VEM33_03830"
     CDS             complement(<1..413)
                     /locus_tag="VEM33_03830"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HYS50983.1"
                     /translation="MNFAGRKALVLGLGDTGLSMAKWLSRAGADVRVADTRASPPRLS
                     ALRALLPSVPAACGPLRQESFAGVDLIAISPGVPLAEPHVRRALAAGTRAIGDIELFA
                     QSSTGRNSRVIAVTGTNGKTTVTSLAGSMARAAGS"
     gene            complement(410..1564)
                     /gene="mraY"
                     /locus_tag="VEM33_03835"
     CDS             complement(410..1564)
                     /gene="mraY"
                     /locus_tag="VEM33_03835"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYS50984.1"
                     /translation="MLLALTQWLAQDVRAFNVFQYITLRAVLATLTALAISLLAGPLV
                     IRKLTAYKIGQAVRDDGPQSHLTKAGTPTMGGALVLLSILTATLLWADLSNRFVWVVL
                     GVTFGFGAIGWVDDYRKVVKNNPKGLSARAKFFWQSLIGIVAAVYLAFAVSASGSGQI
                     LELFSSWVASGFSITLSPKADLIVPLNKNIAIPLGVFGFILLSYLVIVGTSNAVNLTD
                     GLDGLAIMPAVMVAGGLAVFAMVIGRVDYSRYLLYPYIPGAAELTIFCGALVGAGLGF
                     LWWNAYPADVFMGDVGALALGAALGTVAIIVRQEIVLFIMGGVFVAETVSVMLQVLYY
                     KWSGGKRIFRMAPLHHHYELSGWKESQVVVRFWIITMMLVLFGLSTLKLR"
     gene            complement(1565..2944)
                     /gene="murF"
                     /locus_tag="VEM33_03840"
     CDS             complement(1565..2944)
                     /gene="murF"
                     /locus_tag="VEM33_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HYS50985.1"
                     /translation="MEPMMSLREAATAIGARLVGEDVRFDSVSTDSRTLERGALFIAL
                     RGERFDGHQFIGAAKERGAAAAVVEDTDASQAELPFLVVENSRLALGRLAAHWRGRFD
                     IPLVAVIGSNGKTTVKEMIAAILREHFGETRALATEGNLNNDIGLPLTLLRLRRNHRA
                     AVVELGVNHPGETATLAAVASPTVALINNAQREHQEFMKGVAEVAREHGAVFAAMRPE
                     GTGVINADDEFSGYWRGLLAGRRIRDFGLEKPAQVGGRHRFTHFGSEIDLRTPQGATR
                     VELHVDGRHNVLNALAAAACALAADASLEAVARGLAAFRPVGGRMQRRTASSGTKLID
                     DSYNANPDSVRAAIDVLAAEGGAKVLLLGDMGEVGERGAEFHLEIGHYARERGVDRLL
                     AVGELSSACVAAFGEGARHFATVEALISAAQAELRPSTTILVKGSRFMRMERVVQALA
                     GEPSSAGGH"
     gene            complement(2932..>3957)
                     /locus_tag="VEM33_03845"
     CDS             complement(2932..>3957)
                     /locus_tag="VEM33_03845"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HYS50986.1"
                     /translation="AVALHRSLAQLLAAGAQGVAMEVTSIGLEQGRVNGIAFGAALFT
                     NLSRDHLDYHGNMENYARAKQRLFEVPGLKHAVLNLDDVQGARLARMLAGRGLNRAGY
                     SCFAGVAARAGLESHAEALAIEVSPKGIAFDVKSSWGEARIQSTLLGRFNVSNLLGVL
                     TTMLVSGVPFERATAVLAELQPVEGRMQRLGGGGRPLVVVDYAHTPDALEKTLIALKD
                     IARASGGRLVAVFGCGGERDRGKRPLMGAVASRHADAITVTSDNPRGEDPAAIIAEIT
                     AAISVAHEAVEDRRTAIARTIASARTDDVVLIAGKGHEKYQEIAGRRLPFSDALEAEE
                     ALARWSR"
BASE COUNT          711 a         1354 c         1268 g          624 t
ORIGIN      
        1 tcgcttcccg ccgctctcgc catcgatccg gcgagcgaag taacggtggt cttgccgttg
       61 gtgccggtca ccgcgatgac ccgggaattc cggcctgtcg agctttgagc gaacagttcg
      121 atgtcgccga tcgcccgcgt ccctgcggcg agcgcgcgcc gcacgtgcgg ctcggcgagg
      181 ggcactcccg ggcttatcgc gatcagatcg acgccagcga agctctcttg ccgcagcgga
      241 ccgcacgcgg cggggacgga aggaagcaac gctctcaacg cagaaagccg cggcggcgac
      301 gcgcgcgtat cagccacgcg cacgtcagca ccggcgcgcg agagccactt cgccatcgac
      361 agacccgtgt cacccaagcc gagcaccagc gcctttctgc ctgcgaaatt caccgcagct
      421 tcaaagtaga cagaccgaac aggaccagca tcatggtgat gatccagaac cgcacgacga
      481 cctgcgactc cttccacccg gaaagctcgt agtggtgatg gagcggcgcc attcggaaaa
      541 tgcgttttcc cccgctccac ttgtaataga gcacctggag cattaccgag acggtctcgg
      601 cgacgaagac gccgcccatg atgaagagca cgatctcctg gcggacgatg atcgcgaccg
      661 tgcccagcgc cgcgcccagc gcgagtgcgc ccacgtcgcc catgaagacg tccgccgggt
      721 atgcgttcca ccagaggaat cccagacccg cgccgacgag cgcgccgcag aaaatggtga
      781 gctcggccgc accgggaatg taggggtaga gcaggtatcg ggaatagtcg acacgcccga
      841 tgaccatcgc gaacaccgcc aaaccgcccg cgaccatcac cgccggcatg attgcgagtc
      901 cgtccaggcc gtcggtgagg ttgaccgcgt tcgacgtgcc gacgatgacc aagtagctca
      961 gaaggatgaa gccgaacacg cccaaaggga ttgcgatgtt cttgttgagc ggcacgatca
     1021 ggtcggcctt gggcgagagg gtgatggaga atccgctcgc cacccaagac gagaacagct
     1081 cgaggatctg accgctgccc gacgcggaca cggcgaaagc gagatacacc gccgcgacga
     1141 tgccgatcag ggactgccag aagaatttcg ccctcgccga aagccccttc gggttgttct
     1201 tcactacctt gcggtaatcg tcgacccatc cgatcgcgcc gaagccaaag gtgactccca
     1261 gcacgaccca gacgaagcga ttcgacagat cggcccacag cagcgtcgcg gtcaggatcg
     1321 agagcaggac gagcgccccg cccatggtgg gtgtgcccgc cttggtgaga tgggactggg
     1381 ggccgtcgtc gcgcaccgcc tggccgatct tgtaggcggt gagcttgcgg atcaccagcg
     1441 gaccggcgag cagcgagatc gcgagcgcag tgagcgtcgc gagcaccgcg cgcagcgtga
     1501 tgtactggaa cacgttgaac gcgcgcacgt cctgggcgag ccattgggtg agggcgagga
     1561 gcatctagtg ccctcctgcc gaagagggct cgcccgccag agcctggacg acccgctcca
     1621 tgcgcatgaa gcgcgagcct tttacgagga tcgtggtgct cggccgcagc tccgcctgag
     1681 cagcggagat caaggcctcc acggtggcga agtgtcgagc cccttctccg aaggcggcga
     1741 cgcaggcgga agacagctct ccgacggcca acagacggtc tacgccgcgc tcgcgcgcgt
     1801 aatggccgat ctccagatgg aattctgctc cacgctcgcc aacctccccc atgtcgccga
     1861 gcaataggac cttcgcgccg ccttccgctg caagcacgtc gatcgcggcg cgcaccgaat
     1921 ccgggttggc gttgtagctg tcgtcgatga gtttcgtccc cgaggaagcg gtccgccgct
     1981 gcatgcgtcc gccgacagga cggaacgccg caagaccccg cgccaccgct tcgaggcttg
     2041 catccgcggc caaggcgcaa gcggcagcgg ccaaggcgtt cagaacgttg tgccgcccat
     2101 cgacatggag ctcgacccgg gtcgcaccct gcggagtgcg cagatctatc tcgcttccga
     2161 aatgcgtgaa gcggtggcga ccgcccacct gtgcaggttt ctcgagcccg aagtcccgga
     2221 tgcgcctgcc cgcaaggagc ccgcgccaat agccggagaa ttcgtcgtcc gcattgatca
     2281 cgcccgtgcc ctccggccgc atcgccgcga acacggcgcc gtgctcgcgc gcgacctccg
     2341 cgacgccctt catgaactcc tggtgttcac gctgggcatt gttgatgagc gcgaccgtcg
     2401 gagaggcgac ggcggcaagg gtggcggttt cccccggatg attgaccccc agctcgacca
     2461 ctgctgcacg gtggttccgg cgcagtctca gcaacgtcag cggcaggccg atgtcgttgt
     2521 tgaggttgcc ctcggtcgcg agcgcacgcg tctcgccgaa atgctcgcgc agaatcgccg
     2581 cgatcatctc cttgaccgtg gtcttgccat tggagccgat gaccgccacc agcggaatgt
     2641 cgaagcgccc gcgccagtgc gcggcgagcc gtccgagagc gagacgcgag ttctctacga
     2701 cgagaaacgg aagctccgcc tgggaagcat cggtatcctc gacgaccgct gcggcggctc
     2761 cccgttcctt tgcggccccg atgaattggt gcccgtcgaa tcgctccccg cgcagagcga
     2821 tgaacagcgc gccccgctcg agcgtgcggc tgtcggtcga cacggaatcg aagcggacat
     2881 cctctccgac gaggcgcgcc ccgattgcgg tcgcagcctc gcgcaggctc atcatcggct
     2941 ccatcgcgcg agcgcttctt cggcttcgag cgcatcggag aatggcagtc ggcggccagc
     3001 gatttcctga tacttctcgt ggcctttccc ggcgatgagc acgacgtcat cggtcctcgc
     3061 cgaggcgatc gtgcgggcga tcgccgttcg ccgatcctcc acagcctcgt gcgcgacgga
     3121 gatggcagca gtgatttcgg cgatgatggc ggcaggatcc tcgccgcgcg gattgtcgct
     3181 cgtcaccgtg atcgcatcgg cgtgccgcga cgcgaccgct cccatcagcg gccgcttgcc
     3241 gcggtcgcgc tcgcctccgc agccgaacac ggccaccaag cggccgccgc tcgcccgggc
     3301 aatgtccttc agggcgatca gagttttttc gagcgcgtcg ggcgtatgtg catagtcgac
     3361 cacgacgaga ggcctgccgc ctccgcccag gcgctgcatg cgtccctcca ccggctgcag
     3421 ctctgcgagc accgccgtgg cgcgctcgaa gggcactccg gacacgagca tcgtcgtgag
     3481 gactcccagc agattggaca cgttgaagcg cccaagcagg gtgctttgga tgcgcgcctc
     3541 gccccaggaa ctcttcacat cgaatgcgat ccccttcggc gacacttcga tcgcgagcgc
     3601 ctcggcgtgg ctctcgagtc ccgcccgggc ggcgactccc gcgaaacaac tgtagccggc
     3661 gcggttcagg ccgcgcccgg cgagcattcg ggcgagccgc gctccctgca cgtcgtccag
     3721 attgagcacg gcgtgcttaa gccccggaac ttcgaacagg cgctgtttcg cgcgcgcata
     3781 gttctccatg ttgccgtgat agtcgaggtg atcgcgcgag agattggtga acagcgcggc
     3841 cccgaaagcg atgccgttta cgcgcccctg ctcgaggccg atcgaggtga cttccatcgc
     3901 aacgccctgc gcccccgcgg ccagcaattg ggcaagcgaa cggtgcaggg cgaccgc
//
LOCUS       DASPZD010000200         5869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_137083, whole genome shotgun sequence.
ACCESSION   DASPZD010000200 DASPZD010000000
VERSION     DASPZD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137083"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VEM33_03850"
     CDS             <1..528
                     /locus_tag="VEM33_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HYS50987.1"
                     /translation="PTSNLASQLNVVARIISARSSLSANRQVFLVSLGGFDSHDFLLT
                     QHTQQLTIANAAVNAFYGWLVQMGVQNNVTIFTASDFGRTLTSNGDGSDHGWGSHHFV
                     LGGSVLGHEIYGTFPPTTFGTSVDVGQGNMVPGIAVDQYAATFARWLGVSDTDVPLVV
                     PNVVNFGTSRYLPFL"
     gene            715..1335
                     /locus_tag="VEM33_03855"
     CDS             715..1335
                     /locus_tag="VEM33_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYS50988.1"
                     /translation="MNAPRSGKLPSRPASARERIDQTAYELFSRHGIRAVGVDTISAR
                     SGVAKMTLYRHYPSKDDLALAFLRRREELWTRSWLQQEVERRAQAPAERLLAVFDVFE
                     KWFRRRDFEGCSFIKVLLEHDDRRHPVRKAAEAHIETIRAFLRQLAADAGVRGADAFA
                     RQLQMVMMGSIVAAHAGDRNAARRAKETGLLLLASAGIRTGKGARG"
     gene            1332..3698
                     /locus_tag="VEM33_03860"
     CDS             1332..3698
                     /locus_tag="VEM33_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYS50989.1"
                     /translation="MTTYATDGQTRAALLDSQRRVLERIANGAPLPEILETLVRLIEE
                     QAHGMRCAVLLADTQQQRLRFAAAPSIPDDYKAGIDPFLRIAPEMGSCGTAAFLRKPV
                     YTQDTKTDPLWKDCGDVAARNGLRAIWSTPILSDDNAVLGTFAMYCGEPRLPSPEHIQ
                     LIDMATQMARVAIEAKGKEEEIQALSRQREDHLRLVIDTIPTIAWSVRPDGAVDFVNQ
                     RWLEYTGLSSEDALEEAIHTVHPEDLPSVMEKWSVDMAAGEPYEGEMRLRRADGEYRW
                     FLNRTVPLRDEQGNIAKWYGTATDIEDRKRAEDALRKSEEKFRQLAENIREVFWMTTL
                     HFEEMLYVSPAYESVWGRSLESVRKRPQSFMDAIHSEDRERVVGILEGQRQQGFEVEY
                     RIVRPDGSVRWIRDRGFPVKDASGKVYRVAGVAEDITERKRVEMALQKNEQLLSETQV
                     LGRTGSWEHNLVTGEIANTEGNLRLFFGDDHSKGARFEDFAEVVHADDREYVSARHAQ
                     LLAEGGPRDIEYRVVWPDGTVHVLFGRATVVRDAAGRPLRVYGTNVDITERKRAENAL
                     RDSGVQLQALSRRLVELQESERKELARELHDRVGQSLTALKINIDILQPALASQGNAE
                     VLARIADSAALLESTMDTIENVMSELRPPMLDDHGLAAALDWHAKNFSRRTGIAVAVR
                     VNEPAVRPALQVEIALFRVAQEALNNVAKHARAHRVEIALDHANGECVMSVQDDGIGF
                     DGLENASDKPKPGLGMVTMRERAQAVGGRFEVQALPDRGTQLTVRVPS"
     gene            3700..4344
                     /locus_tag="VEM33_03865"
     CDS             3700..4344
                     /locus_tag="VEM33_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYS50990.1"
                     /translation="MTIRVLIADDHGVVAEGLRSLIEAQTDMKVIALVEDGREAVRRA
                     MESNPDVVLMDIAMPVLNGTEATRIIREQRPRTRVIMLSGYTDPVHVYRALRAGAAGY
                     IAKKSVAKEVVDAIRKAHAGKHHLSGQLTESLIDHVVHKAASDEPLQRLSSRERQVLQ
                     MLAEGHAIAKIAGTLYLSPKTVETYRARMMQKLDIRDFATLVRFAIQQGVTPLE"
     gene            4577..5221
                     /locus_tag="VEM33_03870"
     CDS             4577..5221
                     /locus_tag="VEM33_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYS50991.1"
                     /translation="MAIRVFLANSRVILLDSLQVLLEIQQDIRIVGKAVNGHEAVRRI
                     QKLRPDVAVLDTGLVGLNGMEASLKIGNLSPSTAIVMLSTMTSPEIIFRALRAGARGY
                     LSREASGLELVNAVRTVREGKRYLSPKITATLVGDYISEHRSPSPLESLTSRERHILQ
                     LVVEGNPSADVADILALSPKTVDTYRSRIKQKLGIHDLPALVKFAIQHGITSLE"
     gene            5264..>5869
                     /locus_tag="VEM33_03875"
     CDS             5264..>5869
                     /locus_tag="VEM33_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS50992.1"
                     /translation="MSWPDITGHPRRLVLKSGMTERLRILLLEDVADDAELALLELGR
                     SGLHADQRVVESEKSFVDALREFAPDVILSDFSMPGFNGMAALALVREICPDTPFIFV
                     SGTIGEEFAVRALKSGATDYVMKTNLVRLPAVVERALREAKERAAHRKAEGELEALRE
                     RLRSILSTLPDVVWSVAVPSREILYVSPAAATVFGRTQKEVC"
BASE COUNT         1176 a         1807 c         1860 g         1026 t
ORIGIN      
        1 cccacgagca acctcgcctc gcagctcaac gtcgtcgccc gcatcatctc cgcgcggagc
       61 tctctcagtg cgaaccggca ggtattcttg gtcagcctcg gcggcttcga cagtcacgac
      121 ttcctcctca cccagcatac ccagcaactg acgatcgcga acgccgcggt gaacgcgttc
      181 tacggctggc tcgtgcagat gggcgtgcag aacaacgtga cgatcttcac cgcctccgat
      241 ttcggacgca cgctcacctc gaacggcgac gggtccgatc acggctgggg ctcgcatcac
      301 ttcgtgctcg gcgggtcggt cctgggccac gaaatatacg gaaccttccc tccgaccacc
      361 ttcggcacca gcgtcgacgt cgggcaggga aacatggtgc cgggcatcgc ggtcgaccag
      421 tacgccgcga cgttcgcgcg ctggctgggc gtgagcgaca ccgacgtgcc gctcgtcgtt
      481 ccgaacgtgg tgaacttcgg tacctcgcgc taccttccgt tcctctagat cgatcgtcag
      541 ctgcaccgtc ggcgaaaacc gacgcaaaag gcctggaaat ctccaggccc tttttctttg
      601 gtagcgcttg agattgactt caggtccata caggtctgta tagagcatgc tacacttcac
      661 cgattcctta ctagattagc taagtagaca gttctgtata cctttgaggt gaccgtgaac
      721 gcccctcgtt ccggcaagct cccctctcgc ccggcgagcg cgcgtgagcg catcgatcag
      781 accgcctacg agctcttttc ccggcacggt atccgtgcag tgggggtcga taccatcagc
      841 gcgcgctcgg gcgtggcgaa gatgaccttg tatcgccact acccttccaa ggatgacctc
      901 gccctcgctt tcctgcgccg ccgcgaggag ctgtggacgc gttcctggct gcaacaagag
      961 gtggaacgcc gcgcgcaggc accggccgag cgcctgcttg ccgttttcga tgtcttcgaa
     1021 aaatggttcc ggcggcgcga cttcgagggg tgctcgttca tcaaggtgct gctcgagcac
     1081 gacgaccgga ggcacccggt tcgaaaggcc gcggaagccc atatcgagac gatacgcgcg
     1141 ttcctgagac agctggctgc tgatgcgggc gtgcgcggcg ccgacgcatt cgcacggcag
     1201 ttgcaaatgg tgatgatggg ctccatcgtc gccgcccacg cgggcgaccg gaacgcggcg
     1261 cggcgggcca aggagacggg attgctgctc ctcgcaagcg cgggcatcag gaccggcaag
     1321 ggagcccggg gatgacgacc tacgcgaccg acggacagac tcgcgccgcg cttctcgaca
     1381 gccagcgccg cgtgctcgag cggatcgcaa atggcgcgcc gcttccggag attcttgaaa
     1441 cgctcgtcag gctgatcgag gagcaggcgc acggaatgcg ctgcgcggtg cttctcgcgg
     1501 atacgcagca gcagcggctg aggttcgccg ccgcgcccag catcccagac gactacaagg
     1561 ccggcatcga cccgttcctg cgtatcgcgc ccgaaatggg ctcgtgcggg accgctgcct
     1621 tcctgcgcaa gcccgtgtac acgcaagaca ccaagacaga cccgttgtgg aaggactgcg
     1681 gcgacgtcgc ggcccgcaac gggctgcgcg cgatctggtc gacccccatt ctctcggacg
     1741 acaacgctgt gctgggaacg tttgcaatgt actgcgggga gccgcgactg ccctcgcccg
     1801 agcatatcca gctcatcgac atggcgacac agatggcccg cgtggcgatc gaggcaaaag
     1861 gcaaggagga ggagattcaa gcgttgtcgc ggcaaaggga agatcacctt cgcctagtca
     1921 tcgacacgat tcccaccata gcctggagcg ttcggcccga cggcgccgtc gatttcgtca
     1981 atcagcgttg gctggagtat acgggcctgt catctgagga cgcactcgaa gaggcgattc
     2041 acaccgttca tcccgaagac ctaccaagcg tcatggagaa gtggtctgtg gacatggctg
     2101 ccggggaacc gtacgagggc gaaatgcgct tgcggcgcgc cgacggggaa tatcgctggt
     2161 tcctgaaccg caccgtgccg ctgcgcgacg agcagggaaa cattgccaag tggtatggaa
     2221 cggctaccga tatcgaggac cgaaagcgcg ccgaggacgc gctgcgcaag agcgaggaaa
     2281 agttccgcca actagccgaa aatatccggg aggtcttttg gatgaccact ctccactttg
     2341 aggagatgct ctacgtgagt cccgcttacg agagcgtctg gggacgatct ctggaaagcg
     2401 tacgtaaacg accgcaatca ttcatggacg cgatacacag cgaggatcgc gagcgcgtgg
     2461 tcggtatcct tgaggggcag cggcaacagg gatttgaggt ggaatatcgc atcgtgagac
     2521 cggacgggtc tgtacggtgg attcgtgacc gcggatttcc ggtgaaagat gcatccggga
     2581 aggtttaccg agtcgccggc gttgccgagg acatcaccga gcgcaaacgg gtcgagatgg
     2641 ccctgcagaa gaacgagcag ctcctgagcg aaacccaggt cctgggccgc accggcagct
     2701 gggagcacaa cctggtcacc ggcgaaatcg ccaacaccga ggggaacctg cgcctattct
     2761 tcggcgacga tcacagcaag ggcgcgcgct tcgaggattt cgccgaggtc gttcacgcgg
     2821 acgatcgaga gtacgtgagc gcgcggcatg cccagctgct cgccgagggg gggccgcgcg
     2881 acatcgagta ccgcgtggta tggcccgatg gcaccgtcca cgtgctgttc gggcgggcta
     2941 ccgtggtccg ggatgcagcc gggcgtcccc ttcgcgtcta cggaacgaac gtcgacatca
     3001 ccgagcgcaa gcgggctgaa aatgcgttac gggactccgg tgtccagctc caggcgctgt
     3061 cgcggcggct ggtcgagctg caggagtccg aacgaaagga gctcgcgcgc gagctgcacg
     3121 accgtgttgg ccagagcctg accgccctga aaatcaacat cgatatcctc cagccagcgc
     3181 tcgcttcaca gggaaacgcc gaagttcttg cacggatcgc tgattcggcg gcgcttctcg
     3241 agtcgacgat ggatacgatc gagaacgtca tgtccgagct gcgaccgccc atgctcgacg
     3301 accacgggct cgcggcggcg ttggactggc acgcgaagaa cttctccagg cgaacgggga
     3361 tcgcggttgc cgtgcgcgtc aacgagccgg ctgtgcgacc tgcacttcaa gtcgaaatcg
     3421 cgctctttcg cgtcgcccag gaggcgctca acaacgtcgc gaagcacgcc cgcgctcacc
     3481 gcgtcgaaat tgcgctcgat catgcgaacg gcgaatgcgt gatgtccgtg caggacgacg
     3541 gaatcggctt cgatggcctc gaaaatgcct cggacaagcc gaagcccgga ctcggcatgg
     3601 tgacgatgag agaacgtgcg caggcggtcg gcgggcgttt cgaggtacag gcactgcccg
     3661 accgcgggac gcagcttacc gtgcgggttc ccagctgaca tgacgatcag ggttctgatc
     3721 gccgacgatc acggcgtcgt ggccgaaggc ttgcgcagcc tgatcgaggc gcaaaccgac
     3781 atgaaggtca tcgctctcgt cgaggacggc cgcgaggccg tgcgtcgggc aatggagagc
     3841 aatccggacg tggtcctcat ggacattgcc atgcccgttc tgaacggcac cgaggcgacg
     3901 cggatcatcc gggagcagcg cccccggacc cgcgtcatca tgctctcggg gtacacggac
     3961 ccggttcacg tctaccgtgc gctgcgggcc ggcgccgctg gctacatcgc gaagaaatcg
     4021 gtcgccaaag aagtggtcga cgcgattcgc aaggcgcacg caggcaagca ccatctcagc
     4081 ggacaattga ctgaaagcct gatcgaccac gtcgtccaca aagccgcctc ggatgaaccc
     4141 cttcagcgtc tcagctcccg cgagcgacaa gtcctgcaaa tgctagccga aggacacgcc
     4201 atcgccaaaa tcgcgggaac gctttacctt tcaccgaaga ctgtggagac ctaccgcgcc
     4261 cgaatgatgc agaaactcga tattcgcgac tttgcgactc tcgtcagatt cgcgatccag
     4321 cagggcgtta cccctctcga atagccgcga cgcaggcgtt gtcaggaaat atctgacgca
     4381 atttgtcggc tgtacgcgac agcatccgag gctcctcacg attagagttc aggctgagcg
     4441 ccggcgctat ccgccggtct cgccagaggg gttcctaaac cgacttctca aggattccct
     4501 acagacgaag aatcgaaatt cagcaaggat agccaacgcc acgtggggaa gaactgccga
     4561 aaccaggcaa taaagaatgg cgataagagt gtttcttgcg aatagccgcg tcatcctgtt
     4621 ggacagtctg caggtgctgc tggaaataca gcaagacatc aggatcgtcg gcaaggcggt
     4681 gaacgggcac gaagccgtca ggagaatcca gaaactgcgg ccggatgtcg cagtcctcga
     4741 caccggcctg gtcggcctga acggaatgga ggcgagtctc aagatcggca atctttcccc
     4801 gtcaacggca atcgtgatgc tctccacgat gacgtctccg gaaatcattt ttcgcgccct
     4861 tcgggccggt gcgaggggat atctttccag ggaagcgtcc ggattggagc tcgtcaatgc
     4921 ggtccgaacc gtgcgggaag gcaagcgtta tttgagcccc aagatcaccg cgacactcgt
     4981 cggcgattac atcagcgagc accgctctcc gagtcctctc gagagcctga ccagccggga
     5041 gcggcatatt ctccagctgg tggtcgaagg caatcccagc gctgacgtcg ccgacatact
     5101 ggccctgtct cccaagaccg tagacacgta ccggagccgg atcaagcaga agctcggcat
     5161 ccacgattta ccggcgctgg tgaaattcgc cattcagcac ggtatcactt cgctcgagta
     5221 gggagtcctt gacgccgcgt cgggaaaaac ctgacgccga ccgttgagtt ggcctgacat
     5281 caccggacac cctcgtcgtt tagtcttgaa gagcggtatg acagagcgac tcagaattct
     5341 gctgctggag gacgtggccg acgacgccga gctcgcgctg ctcgagctgg ggcgcagcgg
     5401 tttgcacgcc gaccagcggg tggtggagag cgagaagtcg ttcgtcgatg cgctgcgcga
     5461 gttcgcgccg gacgtgatcc tgtctgattt ttcgatgcca gggttcaacg gtatggccgc
     5521 gctcgcgctg gtgcgcgaga tatgcccgga cacgcctttc atcttcgttt cgggaacgat
     5581 cggcgaggag tttgctgtcc gcgcgctgaa gagcggcgcc accgactatg tgatgaagac
     5641 gaatcttgtg cgtctgccgg ctgtcgtgga gcgggcactg cgggaggcga aggagcgagc
     5701 cgcccaccgc aaagcggaag gagagctcga ggcgcttcgc gagcggctgc gcagcatcct
     5761 ctcgacactg ccggacgtcg tgtggtccgt cgcggttccc tcgcgtgaga tcttgtacgt
     5821 cagccccgcc gcggccacgg tcttcggccg aacgcagaag gaggtatgc
//
LOCUS       DASPZD010000201         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_669989, whole genome shotgun sequence.
ACCESSION   DASPZD010000201 DASPZD010000000
VERSION     DASPZD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669989"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VEM33_03880"
     CDS             complement(<1..418)
                     /locus_tag="VEM33_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYS50993.1"
                     /translation="MTKPVAVRTLLGDYPVTLALKKGEVKSSSVSLEFADVKTPSTAF
                     KRVVRSLEFDCAELAIVTFLMAKAHGKPLVLLPAVVLARFQHPHIVYNIERGPLAPDD
                     LAGRRVGTRSYTVTTSTWLRGILANEHGVDIDRVKWV"
     gene            457..645
                     /locus_tag="VEM33_03885"
     CDS             457..645
                     /locus_tag="VEM33_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50994.1"
                     /translation="MSETREVVVVDVKIPFWSMVVLLVKWAIAAIPAMIILLVLATVV
                     SMVLGGVFHWNWMGRGVV"
     gene            726..1970
                     /locus_tag="VEM33_03890"
     CDS             726..1970
                     /locus_tag="VEM33_03890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HYS50995.1"
                     /translation="MKVEESLLANVKMAPRDAILGLTELYVADSNPKKVNLGVGVYYD
                     DQGKVPLLECVRKADQALAASGVARPYLAMDGFPDYVRAVQGLLFGKDHPAVAQGRVT
                     TVQAVGGTGGLKVGADFIRRFAPEAAVYMSDPTYDNHRPLFEEAGYRIDSYPYYDTRT
                     RGIRFPEMTAAMRAIPKGSVLLLHACCHNPTGVDIRGEQWDEVIKIVAERRLIPFLDF
                     AYQGFGDGIEEDAQVARRFAEELPVVLVAMSFSKSFSLYGERVGSLSVVATDSSESAR
                     VLSNLKRMIRTNYSNPPTHGARIVAAVLASPELRALWMKEVVGMRDRVRSVRRELVDR
                     LKKRIPDDFGFILQQRGIFSYSGLSREQMVAMRERYSVYGIESGRICVAALNPRNLDY
                     VADAIAATVGLSKEALASSRVA"
     gene            2017..2811
                     /locus_tag="VEM33_03895"
     CDS             2017..2811
                     /locus_tag="VEM33_03895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HYS50996.1"
                     /translation="MEKPSDRRRFLKSAAALGAAIPAGAAAQSKPAPHSDHDKQHAQH
                     MRAAAAATRPYMFLSQTEVAFLDAAVSHLIPKDELGPGAKEAGVTYFIDQQLFGGYGT
                     MAKKYTQGPWPEGTPQQGYQSPLTPAEVYRAGVRDTNEYCRKTYGKAFDALSRTQQDE
                     VLKGLDGGKIELERVRSQFFFNMLLNNTIQGFFADPVYGGNRDKAGWKLVGFPGVAAV
                     YTTFIEKHGVPYRAQPVSISDITQKVAQVDEHGHAIHVMLDDKAGV"
     gene            2814..>3509
                     /locus_tag="VEM33_03900"
     CDS             2814..>3509
                     /locus_tag="VEM33_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYS50997.1"
                     /translation="MATKLKPVDVVTVGVGLTGTILAKELADAGLKVVGIERGRWRDT
                     DPDFAMPGAHDELKYVIHKELMQDLSKETITFRNAMNETALPMRQLGSFLPGEGVGGA
                     AVHWGGLTWRFLPWDFETKSRTLARYGAKQVAEDCTLQDWGVTYEELEPHFNRFEYLY
                     GVSGKAGNLQGKIIPGGNPFEGSRSKEYPNPPLKTAYTSALFAKAAQSMGYKPFPVPT
                     AANSRAYKNEYGAT"
BASE COUNT          633 a         1179 c         1122 g          575 t
ORIGIN      
        1 tgacccattt cacgcggtcg atgtccacgc cgtgctcgtt cgcgaggatg ccgcgcagcc
       61 acgtcgaggt cgtcacggtg taggaacgcg tgcccacgcg gcgacctgca agatcgtccg
      121 gcgcgagcgg cccgcgctcg atgttgtaga cgatgtgcgg gtgctgaaag cgcgcgagca
      181 ccaccgccgg caggagcacc aacggcttgc cgtgcgcctt cgccatgagg aaggtcacga
      241 tggcgagctc ggcgcagtcg aactcgaggc tccgcaccac gcgcttgaac gccgtgctcg
      301 gcgtcttcac gtcggcgaac tccaggctga ccgaagacga cttcacctcg cccttcttca
      361 gcgcgagggt gaccgggtaa tcccccagca gcgtgcgcac tgcaacgggc ttagtcatgg
      421 cggtgtaccc tcacattttc ccgcaggagg cttccaatga gcgaaacaag agaagtcgtg
      481 gttgtcgatg tgaagatccc gttctggtcc atggtggtcc tgctcgtcaa atgggcgatc
      541 gccgcgatac cggccatgat catcctcctg gtcctggcga ccgtagtgtc gatggtactc
      601 ggaggcgtgt tccactggaa ctggatgggc cggggcgtgg tctagccgcg attcgatcgc
      661 tctccgaccc ttgcgatctt ccgtgattgt ataatcggtt catatttttc agctacagac
      721 gccgcatgaa ggtcgaagaa agcctcctcg ccaacgtgaa gatggcgccc cgggacgcga
      781 tcctggggct gaccgagcta tacgtcgccg attccaaccc gaagaaggtg aacctcggcg
      841 tcggcgtcta ctacgacgac cagggcaagg tgccgctgct cgagtgcgtg cgcaaggcag
      901 accaagctct cgccgcgagc ggtgtggccc gtccctacct ggcgatggac ggctttccgg
      961 actacgtgcg agcggtccag gggttgctct tcggcaagga ccaccccgcg gtcgctcaag
     1021 ggcgggtcac caccgtccag gcggtcggcg gcacgggcgg cctcaaggtc ggcgcggact
     1081 tcatccggcg cttcgctccg gaggccgccg tctacatgtc cgacccgacc tacgacaacc
     1141 accggccgct gttcgaggaa gccggctacc ggatcgacag ctacccctac tacgacacgc
     1201 gcacgcgcgg catccgcttt cccgagatga ccgccgccat gcgcgccatc ccgaagggct
     1261 cggtgctcct gctgcacgcc tgctgccaca accccacggg ggtggacatc cgcggcgaac
     1321 agtgggacga ggtcatcaag atcgtcgcag agcgccgcct catccccttc cttgacttcg
     1381 cctaccaggg attcggcgac ggcatcgagg aggatgcgca ggtcgcgcgc cggttcgcgg
     1441 aggagctgcc ggtggtgctg gtcgcgatgt cgttctccaa gtccttttcg ctctacggcg
     1501 agcgcgtcgg ctcgctctcg gtagtcgcga cggactcgag cgaatcggcg cgggtcctct
     1561 ccaatctcaa gcgcatgatc cgcacaaact actcgaaccc gccgactcac ggcgcgagga
     1621 tcgtcgccgc cgtgctcgct tcgcccgagc tgcgagccct gtggatgaaa gaggtggtcg
     1681 gcatgcgaga ccgcgtgcgc tcggtgaggc gcgagctggt cgaccgcctg aagaagcgca
     1741 ttccggacga cttcggcttc attctgcagc agcgcggcat cttctcgtac tcgggcctgt
     1801 cgcgcgagca gatggtcgcg atgcgcgagc gctactcggt atacggcatc gagtcgggcc
     1861 gcatctgcgt cgccgccctg aatccgcgca acctcgacta tgttgccgac gcgatcgcgg
     1921 cgaccgtggg cctgagcaag gaggcgctcg cctcgtctcg cgtcgcttga gccttacccc
     1981 ggcgcgggtt ccgcgccgat ccatgaagga ggagccatgg aaaaaccgtc agaccgccgc
     2041 cgtttcttaa aatccgctgc cgcgctgggc gctgcgattc cggcgggtgc cgcggctcaa
     2101 tccaagcccg caccgcactc cgaccacgac aagcagcacg cgcagcacat gcgcgcggcg
     2161 gccgccgcga cgcggccgta catgttcctg tcgcagaccg aggtcgcgtt cctcgatgcc
     2221 gcggtgtccc acctcattcc caaggacgag ctggggccgg gcgcgaagga agccggcgtc
     2281 acctacttca tcgaccagca gctcttcggc ggctacggga ccatggcgaa gaagtacacg
     2341 cagggcccct ggcccgaggg cacgccgcag cagggctatc agtccccact cacgccggcc
     2401 gaggtgtacc gcgccggggt ccgcgacacg aacgaatatt gccgcaagac ctacggcaag
     2461 gccttcgacg cgctctcccg cacgcagcag gacgaggtgc tgaaggggct ggacggaggc
     2521 aagatcgagc tcgagcgggt gcggtcgcag tttttcttca acatgctcct caacaacacg
     2581 atccagggct tcttcgccga cccggtgtac gggggcaacc gcgacaaagc aggctggaag
     2641 ctggtcggct tccccggggt cgccgctgta tacactacgt tcatcgaaaa gcacggcgtc
     2701 ccttaccgcg cccagccggt gtcgatctcc gatatcaccc agaaggtcgc gcaggtggac
     2761 gagcacggcc acgcgatcca cgtgatgctc gacgacaagg cgggggtcta gccatggcga
     2821 ccaaactgaa acccgtagac gtcgtcaccg tcggcgtcgg cctcaccggc acgatcctcg
     2881 cgaaagagct ggccgacgcc ggactgaagg tcgtcggcat cgagcgcggc cgctggcgcg
     2941 acaccgatcc cgatttcgcc atgccgggcg cgcacgacga gctgaaatac gtgatccaca
     3001 aggagctgat gcaggacctc tccaaggaaa ccatcacctt ccgcaacgcg atgaacgaga
     3061 cggcgctgcc gatgcgccag ctcggctcct tcctcccggg cgaaggcgtg gggggcgcgg
     3121 cggtgcactg gggcgggctc acctggcgct tcctgccatg ggacttcgag acgaaatcga
     3181 gaacgctcgc gcgctacggc gcaaaacagg tggccgaaga ctgcaccctg caggactggg
     3241 gggttaccta tgaagagctc gagccgcact tcaaccgctt cgagtatctc tacggcgtct
     3301 ccggcaaggc gggcaatctg cagggcaaga tcatccccgg cggcaacccc ttcgagggct
     3361 cgcgctcgaa agagtatccg aacccgccct tgaagaccgc gtatacgagc gcgctgttcg
     3421 ccaaggccgc gcagtcgatg ggctacaagc ccttcccggt gccgactgcc gcgaactcgc
     3481 gcgcctacaa gaacgaatac ggcgcgacg
//
LOCUS       DASPZD010000202         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_996174, whole genome shotgun sequence.
ACCESSION   DASPZD010000202 DASPZD010000000
VERSION     DASPZD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996174"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VEM33_03905"
     CDS             <1..211
                     /locus_tag="VEM33_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS50998.1"
                     /translation="VAAMVQQHPMKVRNLDSVLKNPASYPASSKVPPSIPASTPARRV
                     APKPVPTPSVPSWMKKGGYRNHSDD"
     gene            373..444
                     /locus_tag="VEM33_03910"
     tRNA            373..444
                     /locus_tag="VEM33_03910"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:405..407,aa:Cys,seq:gca)
     gene            complement(647..1282)
                     /locus_tag="VEM33_03915"
     CDS             complement(647..1282)
                     /locus_tag="VEM33_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYS50999.1"
                     /translation="MTNDHYSAPGTKGRVLHSPRSYDLLAWLLLRGKERSFRERLVEL
                     ARLEPGQSVLDIGCGTGSLAITAKRRVGPTGTVQGIDASSEMIARARKKASKAGLDVA
                     FTDGVVEALPFPDRHFDAVLSTLMLHHLPREARQQCAREMRRVTKPGGRVLAVDFGRP
                     AGGRKSFLEHFHRHGGVDLADILKLLSEAGLKIVESGAVGVRDLNFVLATA"
     gene            1431..1997
                     /locus_tag="VEM33_03920"
     CDS             1431..1997
                     /locus_tag="VEM33_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYS51000.1"
                     /translation="MHDKQRTKDRRAEKTEALLREALGALIREKSYDDIVVKEILARA
                     DVGRSTFYAHFHDKDELLLSGIQDMLRSAQSREGIVWFSLPILEHIEEHRNTGGATMG
                     RRGRQAMHEHLQHAIAELIEGDLRRGLHRSRSRVGRGSPELMVRWIATTFVLVLNWWV
                     ENDNRFPAREADRVFRGLIEPTLAEFLT"
     gene            complement(2113..>2332)
                     /locus_tag="VEM33_03925"
     CDS             complement(2113..>2332)
                     /locus_tag="VEM33_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51001.1"
                     /translation="ATTPDPVLLYVRGFDPATRGFRYAVNGRFGSLQSAGGGIIVPFQ
                     IALQGRLTLGRSAVGERARKASGPGPDD"
BASE COUNT          460 a          743 c          696 g          433 t
ORIGIN      
        1 cgtggcggcc atggtgcagc agcatccgat gaaggtccgg aatctcgata gcgttctcaa
       61 aaatccggcg agctatccgg cgagttccaa agtccctccc tcgattccgg cgtcgactcc
      121 ggcccggcga gtcgctccca agccggtgcc gacgccgtca gtgccgagct ggatgaaaaa
      181 gggcggttat cgtaatcatt cggatgactg agaatcatcg gcggtattgc tcgtcgctga
      241 ccttttccat ccactcgaca tttgtgccgt agcgaccgcg ttggccggat cggcttgcgg
      301 cacagttccg gcaaagtcgg acccttctgt aacagttagt ccccacttaa gttctagtcg
      361 cagcaactag ttggcgccgt agccaagtgg taaggcagag gtctgcaaaa cctctattcg
      421 tcggttcgat tccgaccggc gcctctctcg tcaccagcgc tcaatcacat ccttttctcc
      481 gagtggtatc gccgaaagag taggtactta actaccaaca gcatgatggc gcccgacagt
      541 actgcagccg ataaggtcag gtgcgacgag acgtagtaca ggattgcccc gtggccagcg
      601 aggagccccg caaggagcgc cagcggcacc cagaaccgcc ggctcatcac gccgtcgcga
      661 gaacgaagtt gaggtcgcgc acgccgaccg caccactctc gacgatcttt agtcctgcct
      721 cgctcaggag cttgaggatg tccgccaggt caacgcctcc gtgacggtgg aagtgttcta
      781 ggaagctctt gcgaccgccc gcgggtcttc cgaaatccac cgcgagcacg cgcccgccgg
      841 gcttcgttac cctgcgcatc tcgcgggcac actgctggcg cgcttcgcgc ggcaaatggt
      901 gcagcatcag cgtgcttaat accgcgtcga aatgtcggtc cggaaacggc agcgcttcga
      961 cgacaccatc ggtaaaggcg acatccaaac cggccttgct tgccttcttc ctcgctctgg
     1021 cgatcatctc tgaggatgcg tcgattccct gcaccgtccc ggtaggtccg acccgccgtt
     1081 tcgccgtgat cgccagcgat ccggtgccac agccgatatc cagcaccgac tggccaggct
     1141 cgaggcgcgc cagctccacc agccgctcgc gaaacgagcg ctccttacca cgcagcagga
     1201 gccaggccag aaggtcgtag cttcgcggcg agtggagcac tcgccccttg gtcccgggcg
     1261 cggaatagtg atcattcgtc attgtcagaa gatctccgcg taacacgatg aatgcgtatc
     1321 cgtagtatac gggtgggcag cggtgggtga agaacaacaa gctcgctgtg tgcgataatg
     1381 gacaaaccca tggttctgtc ccacatcgcc ggccggagga acgaatcacc atgcacgaca
     1441 aacaacgaac gaaggaccgc cgcgctgaga agaccgaagc gctgctgcgc gaggcactcg
     1501 gggcgctgat tcgcgaaaaa tcctacgatg atatcgtggt caaggagatt ctggctcgtg
     1561 ccgacgtcgg ccggtcaacc ttctatgcgc atttccacga taaggacgag ctgctgctca
     1621 gcggcatcca ggacatgctc cgctcggcgc agtcgcgcga aggaattgtc tggttcagtc
     1681 ttccgatcct cgaacatatc gaagagcatc gcaatactgg aggagcgact atgggccggc
     1741 gggggcgaca ggcgatgcac gaacatcttc agcatgccat cgccgagctc atcgagggcg
     1801 acctcaggag agggttgcac cgctcccgca gcagggtggg tcgtggttca ccggagctga
     1861 tggtccgctg gattgccacg accttcgtgc tcgttttgaa ctggtgggtg gagaatgaca
     1921 accggtttcc tgcgcgcgaa gcagatcgtg tgttccgcgg acttatcgag ccgacgctcg
     1981 ccgagtttct cacctaaagg aggacgcagc ggcatagagt ataccgcagg cgctcctggg
     2041 ctactgcaaa acctctattc gtcggttcga ttccgaccgg cgcctcgctt ccctaggaac
     2101 ccccagtacc actcagtcgt ccggcccggg accgctcgcc ttccgggcgc gctcgcctac
     2161 ggcgctccgc cccaacgtga gccgcccttg cagggcgatc tggaacggga cgatgatccc
     2221 gcccccggca ctttggagcg agccgaaccg gccgttcacg gcgtagcgga accctcgcgt
     2281 cgccgggtcg aagccgcgca cgtacagcag cacggggtcc ggcgtcgtag cg
//
LOCUS       DASPZD010000203         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1226821, whole genome shotgun sequence.
ACCESSION   DASPZD010000203 DASPZD010000000
VERSION     DASPZD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226821"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEM33_03930"
     CDS             <1..213
                     /locus_tag="VEM33_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51002.1"
                     /translation="IREGQREIIRLLSAQQAAAEEHIKKSRESVAESVGLQRLALKRQ
                     RTVTLIAVPGILACIAAIAYLVMRYF"
     gene            230..652
                     /locus_tag="VEM33_03935"
     CDS             230..652
                     /locus_tag="VEM33_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51003.1"
                     /translation="MKPRITLITLGVDDLQRAVRFYRDGLGLKTEGIVGTEFEYGAVA
                     FFDLQAGLKLALWPRKSIAHDSGIALGRPNATDFTIGHNVSSKVEVDAVMEQAKTAGA
                     VVVKPAQDTFWGGYAGYFQDPDGHLWEVAWNPQWVVQE"
     gene            1049..1435
                     /locus_tag="VEM33_03940"
     CDS             1049..1435
                     /locus_tag="VEM33_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016343937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HYS51004.1"
                     /translation="MTVRIVRLGSDRSPDEGLRIGTVRRPPRGVRKSEYASKNFYDIW
                     LPTLAPSAGLVKLAQGAKSERDWNRFMRKYRAEMATLETKAALDLLAALSHQTDFSVG
                     CYCSDESRCHRSILRALLLARGAKVM"
     gene            1444..2073
                     /locus_tag="VEM33_03945"
     CDS             1444..2073
                     /locus_tag="VEM33_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010113212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HYS51005.1"
                     /translation="MLGIHDYWLFVATGVLLNLTPGQDTLYILGRSIAQGRRIGVASA
                     LGITAGSVVHTLAAALGLSAVLATSASAFLVLKVVGAVYLVYLGVRMLITRMAAFGAT
                     DVADSVDTRTAFRQGMLTNVLNPKVALFFLALMPQFIAPDSPTKVGAFLVLGLTFITT
                     GTAWCLVLALGASRVRSFFAGRPRALRGLSQASGALFIVLGLRLVASKQ"
     gene            2097..2576
                     /locus_tag="VEM33_03950"
     CDS             2097..2576
                     /locus_tag="VEM33_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYS51006.1"
                     /translation="MRRIHRSRAALIVVLTLVGCATSPTPGGPADRVDVGAAKRQVAD
                     TERAFARTMATRDHKAFSSFLSQEAVFFSGDKPLRGAQQVADWWKRYYEDPDAPFSWE
                     PEEVEVLDSGTLAMSSGPVRNPKGELIATFTSIWRLEDTGKWRIIFDKGSRACPQKP"
     gene            2600..3091
                     /locus_tag="VEM33_03955"
     CDS             2600..3091
                     /locus_tag="VEM33_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51007.1"
                     /translation="MKLRKMLALSRAVGRNEVKKVEALCSEALAADPGDLMALMALAD
                     AYWRNQRREDALTLALQTLEVQPNEFYALRIVAGVYAERGEHEPAYRYARRLLDADTT
                     HLPPARTVSRILAALAWLPVVRRLKELVDHDERETKSSYSEWLQWANGYVRWYESLSH
                     PAR"
     gene            complement(3151..3729)
                     /locus_tag="VEM33_03960"
     CDS             complement(3151..3729)
                     /locus_tag="VEM33_03960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYS51008.1"
                     /translation="MHKPRARALVLVIGLISGCAALDGLGDTQLTDGVAPVFDPEVPY
                     FPSTDEAVLGMLKLAGTGPDDVVYDLGCGDGRIVISAAKDFGARGVGVEIDPTPLRMA
                     VYHARRAGVEDRVRFVRGDLFEADIGEATVVTLFLFETLNRRLLPKLLRELAPGTRIV
                     AHRYGFGDAWPPERTVQAGASTLYLWTVPKRK"
     gene            complement(3722..>4232)
                     /locus_tag="VEM33_03965"
     CDS             complement(3722..>4232)
                     /locus_tag="VEM33_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYS51009.1"
                     /translation="GTAGEKLISVMIAIATLTSINGAMIVGARSNYALGREWPFLRFL
                     GRWHGASGSPRNAYLVQGAIALLLVGLGAIERKGFQTLVDFTAPVFWAFFLVTGLSLF
                     TLRATDSGAARPYKVPLYPVTPALFVLSCAYMLYSSLAYTGVGALFGVGVLALGAVVM
                     LLGQWAVRA"
BASE COUNT          765 a         1351 c         1323 g          793 t
ORIGIN      
        1 attcgcgaag ggcagcgcga aatcatcagg ctcctgtctg cgcaacaggc cgccgcggag
       61 gaacacatca agaaatccag ggaaagcgtc gccgaatccg tgggcctcca gcggctcgcc
      121 ttgaagcgtc agagaacggt gacgttgatt gctgttcccg gcatccttgc ttgcatcgcg
      181 gcgatcgcat atctcgtcat gaggtacttt tgatctcggc taggggcaca tgaagcctcg
      241 gataacgctc atcactcttg gcgttgacga tctgcaaagg gcggtgcggt tctaccgtga
      301 cggtcttggc ctcaagaccg aaggcatcgt cggcacggag ttcgagtacg gcgctgtcgc
      361 tttcttcgac ctgcaggccg gcctgaagct cgcgctctgg ccgagaaaga gtattgccca
      421 cgattcgggg atcgctcttg gcagacccaa cgcgaccgat ttcaccatcg ggcacaatgt
      481 ctcctcgaaa gtcgaagtcg acgcggtaat ggagcaagcc aaaacggcag gtgcagtcgt
      541 cgtaaagccg gcgcaggaca ccttttgggg tggctacgcg ggctacttcc aagaccctga
      601 cgggcacctg tgggaagtgg catggaatcc ccagtgggtc gtccaagagt gaggttgggc
      661 tgtccgtgct cagaacctgc gttatctaca ctgctgccgc ggctgtaggg atccttgcgc
      721 cttttttcgt tctctgggct gcaagaacag cgccgcctgg cgccagtccg atgtccgtgg
      781 cggcttctgt ggtcctggtg ctggcattcg cttccgcgtt gtttgcttcg ttgcttccgc
      841 gccgctggat aatcactact tctctggtct ctgtgccgat tgcgcttctc ggctcgatca
      901 tgttccttgc gttgactggt ggcagtgcga cttacaccat atggctcgtt gtgggaatcg
      961 gcagcttggt gtcctcggct gtcgcagcgt tttgctgcgc caaagcggtt ctccgacggc
     1021 aatccaagcc gttcggggcg gacgtacaat gaccgttcga attgttcgac tcggttccga
     1081 tcgatctccg gatgaaggcc ttcgcatcgg cacggtgagg cgacctcctc gcggcgtgag
     1141 aaagagcgag tacgcgtcga agaacttcta cgacatctgg ctgccgacgc tcgcccccag
     1201 cgccggactg gtcaagctcg cgcagggagc gaagagcgag cgcgattgga accgtttcat
     1261 gaggaaatac cgtgccgaga tggccaccct tgagaccaag gcggcgctcg acctcctcgc
     1321 cgccctctcc catcagaccg acttctccgt cggctgctat tgctccgacg aatcacgctg
     1381 tcatcgatcg atcctgcgcg cgctgctcct ggcgcgcggt gccaaggtga tgtgagggcg
     1441 accatgctcg gcatccacga ttactggctg ttcgttgcaa ccggggtgtt gctgaacctc
     1501 acgcccggtc aggatacttt gtacattctc ggccgcagca tcgcccaggg acggcggatc
     1561 ggcgtggcct cggccctggg catcaccgcc ggatcggtcg tccacaccct cgccgctgcg
     1621 ctcggattgt cggccgtgct cgcgacgtcg gcctccgcat ttctcgtcct gaaagtcgtc
     1681 ggggcggtgt atctcgtcta cctcggcgtt cgcatgctga tcacgcgcat ggcggctttc
     1741 ggcgcgacgg acgtcgcgga ctcggtcgat acgcggacgg cgttccgcca aggcatgctc
     1801 accaatgtac tcaaccccaa agtggcctta ttcttccttg ccctgatgcc ccaattcatc
     1861 gcgcccgaca gccccacgaa agtcggcgcg ttcctcgtcc tagggcttac gttcatcact
     1921 acgggaactg cctggtgcct cgttctggct ctcggcgcct cgcgcgtgcg gagtttcttc
     1981 gccggtcgcc cgcgtgcact ccgcggcctc tcgcaggcgt cgggggcgct cttcatcgtt
     2041 ctcggcctgc ggcttgtggc aagcaagcaa tgacgggcct cacgggagac tttcgaatga
     2101 gaagaatcca taggagcaga gccgcgctga tcgtcgtcct cacgctggtc ggttgcgcaa
     2161 cgagccccac gcccggaggg cctgccgacc gggttgacgt cggcgccgcc aagcggcagg
     2221 tggcggacac ggagcgtgcc ttcgcacgaa ccatggcgac ccgggatcac aaggccttca
     2281 gctcgtttct gtcgcaggag gccgtcttct tctccggcga caagccgctt cggggcgcgc
     2341 aacaggtggc cgactggtgg aagcgctact acgaggaccc cgatgccccg ttctcgtggg
     2401 agcccgaaga ggtcgaggtg ctcgactccg gcaccctggc gatgagctcc ggtcccgttc
     2461 gcaatcccaa gggagagctc atcgccacgt tcacgtcgat ctggcggttg gaggatacgg
     2521 ggaaatggcg catcatcttc gacaaaggca gccgggcctg ccctcaaaaa ccctaggcgc
     2581 tcggtagaac atcgcgttca tgaaactgcg aaagatgctt gcattgagcc gcgccgtcgg
     2641 gcggaacgag gtcaaaaagg ttgaggccct ctgcagcgag gccctcgcgg ccgacccagg
     2701 agatctcatg gctctgatgg cgctggcgga cgcatattgg cgaaatcaaa gacgtgaaga
     2761 cgctctgacg cttgccctgc agacacttga agtccaaccc aatgaattct acgcattgag
     2821 aatcgttgcc ggcgtctacg ccgagcgcgg agagcacgaa cccgcgtacc gttacgccag
     2881 gcggctcctc gatgcagaca cgacgcattt gccgcccgca aggaccgtct cgcgtatcct
     2941 ggccgccttg gcctggctgc ccgtcgttcg ccgcctgaag gagctcgtcg accatgacga
     3001 gcgggaaacc aaaagctcct attccgagtg gttgcagtgg gcgaacggat acgtgcgctg
     3061 gtacgaatcg ctctctcacc ccgcgcgctg actgcgcgag cttggaccga aggggcctct
     3121 aatgctgagt acgattgccg agccgggacg ctacttgcgc ttcggaaccg tccagagata
     3181 gagcgtgctc gctcccgcct gaactgtcct ctccggcggc caggcgtcgc cgaacccgta
     3241 gcggtgcgcg acgattcgcg ttccgggcgc gagctcgcgc agcagcttag gcagcaggcg
     3301 ccggttgagc gtctcgaaga ggaacagcgt caccaccgtg gcttcgccga tatcggcctc
     3361 gaaaaggtcg ccgcgcacga agcgcacgcg gtcttcgacc ccagcgcggc gagcgtgata
     3421 gaccgccatg cgcagcggcg tcgggtcgat ctcgacaccg accccgcgcg cgccgaagtc
     3481 cttcgccgcg gagatgacga tgcgcccgtc cccgcagccc aggtcgtaga ccacgtcatc
     3541 gggtcctgtg cccgcgagct tgagcattcc caggactgct tcgtccgtgc tcgggaaata
     3601 cgggacctcg ggatcgaaaa cgggcgcgac cccgtcggtg agctgggtgt cgccaaggcc
     3661 atcgagggcg gcgcagccgg aaatcagccc tatcacgagg acgagtgcgc gcgctcttgg
     3721 tttatgcacg gacagcccac tgtccaagca gcattaccac ggctcccaac gccaacacgc
     3781 cgacgccaaa gagtgcgccg acacccgtat acgccaagct cgagtagagc atgtaggcgc
     3841 agctcaggac gaagagcgca ggcgtcactg gatagagcgg caccttgtac ggccgcgcgg
     3901 cgccggagtc cgtggcccgc agcgtgaaga gcgaaagccc agttacgaga aaaaaggccc
     3961 aaaacacggg cgcagtgaaa tcgacgagtg tctggaagcc tttgcgttcg atcgctccca
     4021 gacccacgag caggagcgca atcgcgccct gcacgaggta ggcattgcgc ggcgagccgc
     4081 tcgctccatg ccaacgcccg agaaagcgca ggaacggcca ctcgcggccg agcgcgtagt
     4141 tggagcgcgc gccgacgatc atcgcaccgt tgatcgaggt gagggtcgcg atcgcgatca
     4201 tcacgcttat cagcttctct ccagcggtgc cc
//
LOCUS       DASPZD010000204         6206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_27451, whole genome shotgun sequence.
ACCESSION   DASPZD010000204 DASPZD010000000
VERSION     DASPZD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27451"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VEM33_03970"
     CDS             <1..810
                     /locus_tag="VEM33_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYS51010.1"
                     /translation="LSMLYVRSSSGALVPLNAVAKLTYGVGPMSVSHLGQLPSVTISF
                     DLQQDVALSQAIALIQKAADELQVPPTLNTRFTGTAQAFQASLQGMGILLVLSILVIY
                     LVLGILYESFVHPLTILSGLPTAGLGALVTLWAFNIELNMYAFVGIIMLVGIVKKNAI
                     MMIDFAIEAQRKEGKPAAEAIYQACLIRFRPIMMTTFAALMGSLPIALSFGAGGDARR
                     PLGLAVVGGLLLSQFLTLYITPVIYLYFERLQEWISQRRETRQTAKLRAAD"
     gene            complement(815..1555)
                     /locus_tag="VEM33_03975"
     CDS             complement(815..1555)
                     /locus_tag="VEM33_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYS51011.1"
                     /translation="MKPVTEAELHTYIDGALSEGARAEVEAWLASHPEDAERLRAYAE
                     QNALLRSLYNPVLDEPVPAALLAVRPHAWRRYAAAAAIFALGIGLGWIVRGMFISPQT
                     SPASLARLAATAHAVYSPEVRHPVEVTANEEEHLVAWLSKRLGTTLKAPKLAPLGYEL
                     VGGRLLSGPQGPVAHFMYQDARGQRLTLYVSRQRGDPRDTAFRFSQEDRVSVFYWVDG
                     NFGYALSGEIRREQLLQVADVVYKQLNP"
     gene            complement(1552..2049)
                     /locus_tag="VEM33_03980"
     CDS             complement(1552..2049)
                     /locus_tag="VEM33_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYS51012.1"
                     /translation="MDRRLIIEHIPRLRRYARALVGDRYVADDLVQDTLERAWNKFYL
                     WRPGSDLRAWLFTIMHNVFVNQARRRRYEIEQAMEEVPAVSVRATQGEQLELQDVDRV
                     LRSLPPEQREVVLLVAVEQLTYEEVSGALNIPIGTVMSRLSRARERMRQLLGGQTVIP
                     LKVVK"
     gene            complement(2356..2988)
                     /locus_tag="VEM33_03985"
     CDS             complement(2356..2988)
                     /locus_tag="VEM33_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYS51013.1"
                     /translation="MKRALALLSFAVCAGPLAAAEPAAKPDSVKGQSIAAEVCAACHG
                     ADGNSPSSANPKLASQVPEYLQKQLANFKAAAGKRAERESPIMGGMVAGLSTADMRDV
                     AAHYAAQRAKPGAVKSQDALALGRKIWRGGDMAKGLPACAGCHGASGAGMPAQYPRLA
                     GQYAEYTEAQLKAFRSGERRNDANKMMQAIAARMSDPEIRAVADYIAGVR"
     gene            3055..3774
                     /gene="yihA"
                     /locus_tag="VEM33_03990"
     CDS             3055..3774
                     /gene="yihA"
                     /locus_tag="VEM33_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HYS51014.1"
                     /translation="MSPFRSASFFKSVASLADAPASIGEIAFVGRSNTGKSSAFNALA
                     GSQLAFVSKTPGRTRLINFYALGSERFIVDLPGYGYARVPEATRASWEKLLGGYLQTR
                     APLCGLALVMDVRHPLTELDQRMLGWFCPTGKPVHVLLTKADKLSRESAQKVLRRVEG
                     EIGAFAPNASVQLFSSLKKSGLEEAQAVLLRWLDASAPRAVGVALGREPNETPRQDRG
                     WRNKSPRAKRGNQPGAKCLKV"
     gene            3914..4921
                     /gene="hemB"
                     /locus_tag="VEM33_03995"
     CDS             3914..4921
                     /gene="hemB"
                     /locus_tag="VEM33_03995"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYS51015.1"
                     /translation="MQVYGSFPAVRMRRMRRDEFSRRLMREHVLSPDDLIYPVFVIEG
                     KGRTQAVPSMPGVSRYTADKLTAVAERCLKLGVPVMALFPAIEPSLKTPDGKEATNPD
                     GLIPRVVGALKKRFPELGIMTDVALDPYTDHGQDGLVDSGGTILNDDTLAVLEEQALV
                     QAAAGVDIVAPSDMMDGRIGRIRIALEEKEFIHTRIMAYSAKYASGFYGPFRDAVGSA
                     ASLGKGNKFTYQMDPANSDEALWEVGLDLQEGADMVMVKPGMPYLDIVRRVKDEFKAP
                     TYVYQVSGEYAMLKAAFKNGWLTEKQCVMEALVAFKRAGADGILTYFALDAAGWLKEE
                     R"
     gene            complement(4926..>6206)
                     /gene="dsbD"
                     /locus_tag="VEM33_04000"
     CDS             complement(4926..>6206)
                     /gene="dsbD"
                     /locus_tag="VEM33_04000"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HYS51016.1"
                     /translation="EDVRFAGSLESRRLWPVMAVFFGAGLLRTFTPCVLPMIPILSGI
                     IVGEGRNLTRRRALLVSLAYVFGMAVTYTAIGIAAALSGSLLSAALQNAWVLGGFAAI
                     FVVLALSMFGFYELQLPSRWQMRIVQISNRLGGGHWGAVMLMGMLSAAIVSPCVTAPL
                     AGALLFMSQTRDAILGGTALFSMAIGMGVPLVLVGVSGGLVLPKSGHWMNAVKQFFGV
                     LLLAVAIWIIAPVIPLAVQMFLWAMLLIGSGVFLGALEPLGAGASGWDRLWKAFGIIA
                     LLAGVAQAVGALSGARDPLRPLSGAFSDSPAQKPLPFEAVKAPDLDARLKTANKVAML
                     DFYADWCVSCKEMERFTFSDPQVQARLGQMVLLRADVTANTADDKALLKRFRLFGPPG
                     IIFFDARGREIEGLRVIGYQSPEKFIKSLDLALR"
BASE COUNT         1180 a         2022 c         1929 g         1075 t
ORIGIN      
        1 ctgtcgatgc tttacgtgcg ttcatcgagc ggggcactgg tgccgctcaa cgcggtggcg
       61 aagctcactt acggcgtcgg ccccatgagc gtgagccacc tcggccagct gccctccgtc
      121 acgatttcgt tcgacctgca gcaagacgtg gccctctccc aggcgatcgc actgatccag
      181 aaggcggcgg acgagctgca ggtgccgccc accctgaaca cgagattcac gggtacggcg
      241 caggcgtttc aggcctcgct ccaagggatg ggcatcctgc tggtgctctc cattctggta
      301 atctacctgg tgctcggcat tctgtacgag agcttcgtcc acccgctcac gatcctctcc
      361 ggcctgccga ctgcgggact cggagcgctg gtgaccctgt gggccttcaa catcgagctc
      421 aacatgtacg ctttcgtcgg catcatcatg ctggtcggaa tcgtcaagaa gaacgcgatc
      481 atgatgatcg acttcgcgat cgaggcgcag cgcaaggagg gcaagcccgc cgccgaggcc
      541 atctaccagg cctgcctgat ccgcttccgg cccatcatga tgaccacgtt cgccgccctc
      601 atgggaagct tgccgattgc gctctctttc ggcgcgggcg gagacgcgcg ccgtccgctg
      661 ggactcgcgg tggtgggcgg gctcttgctg tcgcagttcc tgaccctcta catcacaccc
      721 gtgatctacc tctacttcga gcgccttcag gagtggatct cgcagcggcg cgagacgcgc
      781 cagacggcaa agttgcgcgc cgccgactag gcgatcacgg attgagctgc ttgtagacga
      841 cgtctgcgac ctggaggagt tgttcccttc ttatctcccc ggaaagcgcg tacccgaaat
      901 tgccgtcgac ccagtagaag accgacacgc ggtcctcctg gctgaagcgg aaagcggtgt
      961 cgcgcggatc ccctctttgc cgggagacgt agagcgtgag gcgctggccg cgcgcatctt
     1021 gatacatgaa gtgcgcgacc ggcccctgcg gtcccgaaag tagccgcccg cctacgagtt
     1081 cgtagccaag cggggcgagt ttcggcgctt tcagcgtggt gccgaggcgc ttcgacagcc
     1141 atgccaccag gtgttcttcc tcgttggcgg tcacctccac cggatggcgc acctcgggcg
     1201 aatacaccgc gtgggcagtc gccgccaagc gtgcgagcga cgccggcgac gtctgcgggc
     1261 taatgaacat cccgcgaacg atccagccaa gcccgattcc gagcgcaaat atcgcagctg
     1321 ccgcggcgta gcgccgccac gcgtgcgggc gcaccgcaag cagcgccgcc ggcacgggct
     1381 cgtcgagaac tggattgtag agcgaacgga gcagggcgtt ttgctcggcg tacgcgcgca
     1441 gccgttcggc gtcctcgggg tgagaagcga gccaggcctc gacctctgcc cgagcgccct
     1501 cggaaagggc gccgtctatg taggtgtgca gttcagcctc ggtaaccggc ttcacttcac
     1561 gactttcagg ggtatcacgg tctggccgcc gagcagctgc cgcatgcgct cgcgcgcccg
     1621 cgacagtcgc gacatgacag tgcctatggg gatattcaga gcaccgctga cctcctcgta
     1681 agtcagctgc tccactgcca ccagcagcac gacctcccgc tgctccggcg gcagcgagcg
     1741 cagcactcgg tccacgtcct gcagctcgag ttgctcgccc tgggtcgcgc gaaccgacac
     1801 cgcgggcact tcttccatcg cctgctctat ttcgtagcgg cggcgccttg cctgatttac
     1861 gaacacgtta tgcatgatcg tgaacagcca cgccctcaga tcgcttcccg ggcgccagag
     1921 atagaacttg ttccacgcgc gctccagggt gtcttgaaca aggtcgtcgg cgacgtagcg
     1981 gtcaccgacg agagcgcggg cgtagcgccg cagccggggg atgtgctcga tgatgaggcg
     2041 acgatccatc tcccgcacat tctagcgcgc ggcaagtttt ttcgggaacg ctccatccgc
     2101 cgggaaacgc gcgacttgcc cactgctccc ccctccaaaa aaagaaccta cgagacaaac
     2161 gcgcggcttg gcgcgtttat tccgcaggtt gcggccgccc cgtggaattc cgccgggcgc
     2221 ggcctgcgta aacacgatac tagctcccgc tattccagct cgggtggcgc tcggaaaggc
     2281 gccctttgtg aagacgcgaa acgaaaaggg tggccgggcc accctcgcgt caggtcgtcg
     2341 gcaacgtgag gacgatcagc gcacaccggc gatgtaatcg gccaccgcgc gaatttcagg
     2401 atcgctcatc cttgcggcga tcgcttgcat catcttgttc gcgtcgttcc tgcgctcgcc
     2461 cgagcgaaac gccttcagct gcgcttcggt gtattcggcg tactgtcccg cgagacgcgg
     2521 gtattgcgca ggcatgccgg ccccgctcgc gccgtggcat ccggcacacg caggaaggcc
     2581 cttggccatg tcgccgcccc gccagatttt tcgaccgagc gcgagcgcat cctggctttt
     2641 caccgcgccc ggcttggccc gttgcgcggc gtaatgcgcc gccacatcgc gcatgtctgc
     2701 ggtggagagg cctgcaacca tgccgcccat gatcgggctt tcccgctcgg ccctctttcc
     2761 ggccgcggcc ttgaaattgg cgagttgctt ctgcaggtat tcgggtactt gactggcgag
     2821 cttcggattg gcggagctcg ggctgttgcc gtcggcaccg tggcaagccg cgcaaacctc
     2881 ggcggcgatg ctttggccct tgacggagtc aggtttcgcc gcgggttccg cggcggcgag
     2941 cgggccggcg caaactgcga aactcaaaag cgccaacgct cgtttcatgc ctttttccct
     3001 ccgcgagcct tcgctcaaaa ggctgatatt ctagcaaaac cagttacccg acccatgtca
     3061 cccttccgca gtgcttcctt cttcaagagc gttgcctcgc tagcggacgc tccggcgagc
     3121 atcggtgaaa tcgctttcgt cggtcgatcg aacacgggca aatcaagcgc cttcaacgcg
     3181 cttgccggga gccagctggc attcgtgagc aagacccccg gacgcactcg gctcatcaat
     3241 ttctacgcgt tgggctcgga gcgattcatc gttgatctac ccggctacgg ctacgcccgg
     3301 gtccccgagg cgacgcgggc gtcctgggaa aaactgctgg gcgggtacct ccagacgcgc
     3361 gcgcctttgt gcggtttggc cctggtaatg gacgtgcgcc acccgctgac cgagctcgat
     3421 caacgcatgc tcggatggtt ttgtcccacg ggaaaaccgg tgcacgtgtt gctcaccaaa
     3481 gcggataaac tctctcggga gtccgcccag aaggtcttgc gcagggtcga aggggaaatc
     3541 ggcgcgttcg cgcccaacgc ctcggtgcag ctgttctcga gcctcaagaa atccggactt
     3601 gaggaggctc aagcggtgct gctccggtgg ctggacgcga gcgcgccaag ggccgtcggc
     3661 gtggcccttg gccgagagcc aaatgaaacc ccccggcaag accgggggtg gagaaataaa
     3721 agcccccggg ctaaaagggg aaatcagccc ggggcaaaat gccttaaggt ttaaggcatc
     3781 ccgctcaggg aggagaagcg ggagacgacc gaagtcgcct acagtatggg agtcctctcg
     3841 aggagaaagg ttcccgtagc gggtcttcca aaagcataac aggctgatcc gccttggaaa
     3901 aaaaggagcg atcatgcagg tgtacggcag cttccccgcg gtgcgcatgc gccggatgcg
     3961 gcgcgacgag ttctcgcgcc gcttgatgcg cgagcacgtg ctcagccccg atgatctgat
     4021 ctacccggtc ttcgtgatcg agggcaaggg ccgcacgcag gccgttccat ccatgcccgg
     4081 ggtgtcgcgt tacacggccg acaagctcac tgcggtggcc gaacgatgcc tgaagctcgg
     4141 tgtgccggtg atggcgctct tcccggccat cgaaccgtcg ctcaaaacgc ccgacggcaa
     4201 ggaagcaacg aatcccgacg gactgatccc gcgcgtcgtc ggagcgctga aaaaacgctt
     4261 tcccgagctc ggcatcatga ccgatgtcgc gctcgatccc tataccgatc acgggcagga
     4321 cgggctggtt gattcaggcg gcactatcct caacgacgac acgctcgccg tactcgagga
     4381 gcaggcgctc gttcaagcgg cggcgggcgt ggacatcgtc gcaccttcgg acatgatgga
     4441 cggccgcatc ggccgcatcc ggatcgcgct ggaggagaaa gaattcattc acacgcgcat
     4501 catggcgtat tcggccaagt acgcttcggg attctacgga cccttccgcg acgcggtcgg
     4561 gtcggcggca agtctgggca agggcaacaa gttcacctac cagatggacc ccgccaacag
     4621 cgacgaggcc ctttgggaag tggggctgga tctgcaagaa ggcgccgaca tggtgatggt
     4681 gaagccgggc atgccctatc tcgatatcgt gcgccgggtc aaggacgaat tcaaagcacc
     4741 gacctacgtc tatcaggtga gcggcgagta cgcgatgctg aaagcggcat tcaagaacgg
     4801 atggctcacc gagaagcagt gcgtgatgga agcgctcgtc gcattcaagc gcgcaggcgc
     4861 cgacggcatc ctgacctact tcgcgctcga tgcggcggga tggttgaagg aagagcgcta
     4921 ggcccctagc gcaatgccag atcgagcgac ttgatgaatt tctccggcga ctgatagccg
     4981 atcacgcgca gaccctcgat ttcccggccg cgggcgtcga agaagatgat cccgggcggt
     5041 ccgaacaggc gaaagcgctt gagcagcgcc ttgtcatcgg ccgtgttggc tgtgacatcg
     5101 gcgcgcagga gcaccatctg gcccaggcgg gcctgtacct gcggatcgct gaaggtgaag
     5161 cgctccattt ccttgcacga cacgcaccag tccgcataga aatcgagcat cgcgactttg
     5221 tttgccgtct tcaggcgcgc gtccagatcg ggcgctttga ccgcttcgaa cggcagcggc
     5281 ttttgcgccg gtgaatccga aaaggcgccg gaaagaggcc gcagcggatc tcgcgctccc
     5341 gataacgccc cgacggcctg cgcgacgcca gcaagcaagg caatgatacc gaatgccttc
     5401 catagccggt cccatcccga cgcgcccgcg cccaaaggct cgagcgcgcc caggaatacc
     5461 cctgatccga tgagcagcat cgcccagagg aacatctgca ccgccagcgg gattaccggc
     5521 gcgatgatcc agatcgctac ggcgagaagg agtacgccga agaactgctt gaccgcgttc
     5581 atccagtgac cggacttcgg cagaaccaag cctccggaaa cgcccacgag tacgagcggt
     5641 acgcccatgc cgatcgccat cgagaagagc gccgtaccac cgaggatcgc gtcgcgcgtc
     5701 tggctcatga agagcagcgc ccctgccagc ggcgcggtca cgcaaggcga aacgatcgcg
     5761 gccgacagca tacccatcag catcaccgca ccccagtgcc cgccgcccag gcgattactg
     5821 atttggacga tccgcatctg ccatcgcgaa ggcagctgca gctcgtagaa gccgaacatc
     5881 gacaaggcga gcacgacaaa gatcgctgcg aagccgccga gcacccaggc gttttgcagt
     5941 gccgccgaaa gcaggctccc tgaaagcgcc gccgcgatcc cgattgccgt gtaggttacg
     6001 gccatgccga atacgtaagc gagcgagacc agcagcgcac gccgccgcgt gaggtttcgt
     6061 ccctcaccga cgatgatccc agagaggatc gggatcatcg gaagtacgca cggcgtgaac
     6121 gtgcgcagaa gcccggcgcc gaaaaacacc gccatgaccg gccacagccg acgcgattcc
     6181 agactacccg cgaaacgcac gtcctc
//
LOCUS       DASPZD010000205         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_826357, whole genome shotgun sequence.
ACCESSION   DASPZD010000205 DASPZD010000000
VERSION     DASPZD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826357"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VEM33_04005"
     CDS             <1..277
                     /locus_tag="VEM33_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51017.1"
                     /translation="GVSRGLDSWAKNALRGRNGSIPLITEHSAGQNNRNAAHPSAAAN
                     VPQARTPSGFDWSDAGVGACGALGLVLVAGAGMLALRRRDALAHLNA"
     gene            complement(356..>2226)
                     /locus_tag="VEM33_04010"
     CDS             complement(356..>2226)
                     /locus_tag="VEM33_04010"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51018.1"
                     /translation="LNATAILLEPLSEDESSQLIDNLAGTRLDAPTRQKIIAAAEGNP
                     LFVEEMLALALEDGHSDGALKMPPTIQALLAARLDRLGDVERLILERASVEGKVFHQS
                     AVAELTPDAFRQAVPSSVAALVRKELIRPNRALFVGEQAFRFRHLLIRDAAYDSIPKA
                     ARSDLHERHAAWLELEAGERIVEYEEIIGYHLEQAFRYRSDLGPLDESARRLARRAAE
                     RLGAAGRRAFARSDAPAAVNLISRAAALLPVDDPARVDLVPNVRVVQGMSGNLSWAER
                     ILSEALETGDARLKAHALVQRGFLRLFTEPEITPDDLIDGATKALAVFERLGDDLGLA
                     RAWRLVAQAHYLGRRGGACAEASEAALMHAVRAQDSFEVKEIVEWLAIALTLGSTPAS
                     EALKRCEELTDVAAGDRFLEVTLFSVRAYLEAMQGRPAEAKTLLATADRAAGDRAYLN
                     RIPYFSIYLGLVHILAGEEAGAERPLRDGCKALEEVGERTNFCSVAALLGRVLCAQGR
                     YPEAEAFTRESEKATRPNDVLSNVMWRSVRATVRAQQGELDKAEILAREAVGFAALSD
                     FVNVHGDALVALADVLRLAHRPEEANDALQGAAELYEQKGNAVSAAGARSFLAAHA"
BASE COUNT          396 a          740 c          718 g          372 t
ORIGIN      
        1 cggcgtcagc cgcggactcg attcctgggc caagaatgct ctccggggcc gcaacggctc
       61 gattccgttg atcaccgagc actcggccgg tcagaacaac cggaatgcag cccacccgag
      121 cgcggccgcg aacgtcccac aggcgcgaac gccgagcggc ttcgactgga gcgacgcggg
      181 ggtcggtgcc tgcggcgcgc tgggactggt tctggtagcc ggcgccggga tgctggccct
      241 tcgaaggcgc gacgctctgg cacacctgaa cgcctgaagc agcatcaaga tcgcgggcgc
      301 caagtcgtag gcgggccacc tggctcgcct acgactacgg ttggcgaaca gcgcgctacg
      361 cgtgcgccgc gaggaacgag cgagccccgg cggctgagac cgcgtttccc ttctgctcgt
      421 agagctcggc cgcaccttgt agcgcgtcat tggcctcttc gggcctgtgc gcaagtcgca
      481 gcacatcggc tagagcgacc agggcgtcgc cgtggacgtt gacgaagtca ctcagtgcgg
      541 caaagcccac cgcctcacgt gcgaggatct cggccttgtc gagctcccct tgctgtgcgc
      601 gcacggtcgc acgcactgac cgccacatca cattcgacag aacgtcattc ggacgcgtcg
      661 ctttctcgct ctcccgcgta aacgcctcgg cctccggata tcgtccttga gcacacagca
      721 cccgcccgag gagggcggcg accgagcaga agttggtcct ctcgccgacc tcctcgagcg
      781 ctttgcagcc gtcccgaagt gggcgttccg ccccagcttc ctcgcctgcg agtatgtgga
      841 caagccccag atagatcgag aagtagggga tcctgttgag gtacgcgcga tctccggccg
      901 cccgatctgc ggtggccaaa agcgtcttcg cttcggccgg gcggccttgc atcgcctcga
      961 ggtaagcccg aaccgaaaaa agcgttactt caaggaaccg gtcgccggcg gcgacgtcgg
     1021 tcaattcctc gcaacgcttg agcgcctcgg aagccggagt cgaaccgagg gtgagtgcga
     1081 tcgcgagcca ttcgacaatc tccttcacct cgaacgaatc ttgcgcacga accgcatgca
     1141 tcagtgcggc ctccgacgcc tcagcacacg cgcctcctcg gcgcccgaga tagtgcgcct
     1201 gcgcgacgag acgccaagcg cgcgccaggc ccaggtcatc acccaagcgc tcgaagactg
     1261 ccagcgcctt cgtcgcgccg tcgatgaggt cgtcgggcgt gatctcgggc tcggtgaaca
     1321 ggcgcaggaa tccacgctgc acaagggcat gagccttgag tcgcgcgtcg ccggtttcga
     1381 gcgcctcgct aaggatgcgt tcggcccagc tcaaatttcc gctcatgccc tgcacgacgc
     1441 gcacgttggg aaccaggtcg acgcgcgcgg gatcgtcgac aggtaacagc gcagccgctc
     1501 tcgagatgag gttgacggct gccggagcgt cgctgcgtgc aaatgcgcgt cgaccagcgg
     1561 ccccgaggcg ttccgccgcc cgacgagcaa gccgacgcgc cgattcgtcg agaggaccga
     1621 gatccgatcg gtaccggaaa gcttgctcaa ggtggtagcc gatgatttcc tcgtactcga
     1681 cgatccgctc acccgcctcc agctcgagcc atgctgcgtg gcgctcgtgc agatcggagc
     1741 gcgccgcctt tgggatcgag tcatacgcgg catcgcggat gagcagatgt ctgaaacgaa
     1801 aggcctgctc gccgacgaag agagcgcggt tcggacggat caattccttt cgcacgagcg
     1861 ccgcgacgct tgatgggacg gcctgccgaa atgcgtcggg cgtgagctcg gcgaccgcgc
     1921 tctggtgaaa gaccttgccc tcgacggacg cgcgctcgag aatcaagcgc tcgacgtctc
     1981 cgagtcgatc gagacgcgca gcgaggagtg cctggatcgt cggaggcatc ttgagcgcgc
     2041 cgtctgagtg cccatcttca agcgcaagcg cgagcatctc ctcgacgaag agaggattcc
     2101 cttcagcagc cgcgatgatc ttttgccgcg tcggggcgtc aagcctggtg ccggcaaggt
     2161 tgtcgatcag ctgcgagctc tcgtcttccg agagtggctc gagcaaaatc gcggtcgcgt
     2221 tgagtt
//
LOCUS       DASPZD010000206         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_561337, whole genome shotgun sequence.
ACCESSION   DASPZD010000206 DASPZD010000000
VERSION     DASPZD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561337"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..791)
                     /locus_tag="VEM33_04015"
     CDS             complement(243..791)
                     /locus_tag="VEM33_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS51019.1"
                     /translation="MGKESHYSSKKDRVFVRGIQGQYSLKDELARLRGLPRVIKGKDL
                     LFNDGPQSFSKHFIEPVDGMTQTLHVHLEEYGPGGRTQKHGHVNEAAFYILDGKGYEI
                     HDGVRYDWSAGDVAIVHMNCVHQHFNADPVKPARALVLKPKPMILFMNLMFQQTVIPR
                     PKEATPEGTGFVPRHHEENYNH"
     gene            complement(809..1864)
                     /locus_tag="VEM33_04020"
     CDS             complement(809..1864)
                     /locus_tag="VEM33_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS51020.1"
                     /translation="MPGSSLVASLLAAGAVLSAPVIAQVKPNTTAAEVGLYAGPDRLR
                     KLIEGAKQEGELNLYTSAQSDDMGALVGAYEKKYGVKVSIWRASSEKVLQRAVAEARA
                     NRNTVDVVETNGPEMESLHRERVLQQVKSPHHADLIAPAIRPHGEWVGTRLNVFVQAY
                     NTQAVRKQDLPKTWEDLLEPKWKGKLGIEQEDSDWLAGLFAEIGEAKGTKLFKEIVAK
                     NGMSVRKGHTLLAQLVVSGEVPLALTVYNYKAEQFKSKGAPIDWFSIGTAIARPNGVG
                     VARRAPHPHAAVLFYDFEIGEEGQKILAQRDFVPTNRKIDTPLNKLPLKFVDARVTLD
                     EYDKWVKLYEEIFGKGR"
     gene            complement(1867..>2485)
                     /locus_tag="VEM33_04025"
     CDS             complement(1867..>2485)
                     /locus_tag="VEM33_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51021.1"
                     /translation="FNFFTIAYNTNLVKANEVPNTYEDLLNPRFAGRVGLEASDVDWF
                     GAVVKSMGEEKGLAFFRKLAAAKPEMRSGHTLMAELVASGEIPLAATIYNHNAERLVV
                     KGAPIRWKALKPTFGRPNAIGVTRRAPHPHAALLFADFMLSLEGQTILKERNRVPSSR
                     AVDTHLNKFPFEMIDPVITLDEADRWEKLWSDLFLKGQKVQKEAE"
BASE COUNT          372 a          788 c          783 g          542 t
ORIGIN      
        1 agctcccagg gcatctcttc cggatgcacg atcttcttgc gcttggcgtt gcgcgccggc
       61 gcctgcatcg cgttctcgag caggctctga tacagcttct ggtgagcgct gccgcgcatc
      121 cacgctttcg attcacccca ggccatatgc gactcctttt ctcaagcggc ggtcaaaccg
      181 gggacagacc acgttttcca tgttctgaga gagactgcga aaaacgtggt ctgtccccgg
      241 tttcaatggt tgtagttctc ctcgtgatgc cgcggaacga agcccgtgcc ctcgggcgtc
      301 gcttccttcg gccgcgggat gacggtctgc tggaacatca ggttcatgaa caggatcatc
      361 ggcttcggtt tgagcacgag cgcgcgggcg ggcttcaccg ggtccgcgtt gaaatgctga
      421 tgcacgcagt tcatatggac gatcgcgacg tctcccgcgg accagtcgta gcgcacgccg
      481 tcgtggatct cgtagccctt gccgtcgagt atgtagaacg cggcctcgtt tacgtggccg
      541 tgcttctgcg tgcggccccc ggggccgtac tcctccaggt gcacgtgcag cgtctgcgtc
      601 atgccgtcga ccggctcgat gaagtgcttg ctgaaggact gaggaccgtc gttgaagaga
      661 aggtccttgc ccttgatcac gcgcggcagg ccccgcaggc gcgcgagctc gtccttgagg
      721 ctgtactgcc cctggatgcc gcgcacgaag acgcggtcct tcttgctgct gtagtgcgat
      781 tccttgccca taggcttgct ccgttcgctt accgaccttt gccgaaaatc tcctcgtaca
      841 gcttcaccca cttgtcgtac tcgtcgagcg tgacgcgcgc gtcgacgaac ttcagcggca
      901 gcttgttgag cggcgtgtcg attttcctgt tcgtcggcac gaagtcgcgc tgcgcgagaa
      961 tcttctggcc ttcctcgccg atctcgaagt cgtagaagag caccgcggcg tgcggatgcg
     1021 gggcgcggcg cgcgacgccg actccgttcg gccgcgcgat cgccgtgccg atcgagaacc
     1081 agtcgatcgg cgcgcccttg ctcttgaact gttctgcctt gtaattgtat acggtcagcg
     1141 cgagcggcac ttcgcccgac acgacgagct gcgccagcag ggtgtggccc ttgcgcaccg
     1201 acatgccgtt tttcgccacg atttccttga aaagtttcgt tcctttggcc tcgccgattt
     1261 ccgcgaacag gcccgcgagc cagtccgagt cctcctgctc gatgccgagc ttgcctttcc
     1321 acttcggctc gagcaggtct tcccaggtct tcggcagatc ctgcttcctc acggcctggg
     1381 tgttgtaggc ctgcacgaac acgttgaggc gtgttcccac ccactcgccg tgagggcgta
     1441 tcgccggcgc gatgagatcg gcgtggtgcg gcgacttgac ctgctgcagg actctttcgc
     1501 ggtgcagcga ttccatttcg ggcccgttgg tctcgaccac gtccacggtg ttgcggttgg
     1561 cgcgcgcttc ggcgacggcg cgctgcagca ccttctccga gcttgcgcgc catatgctga
     1621 ccttcactcc gtatttcttc tcgtaggcgc cgaccagcgc tcccatgtcg tcggactgcg
     1681 ccgaggtgta gaggttgagc tcgccctcct gcttcgcccc ctcgatgagc ttgcgcaggc
     1741 ggtcgggacc cgcgtacagg ccgacctccg cagccgtggt gttcggcttg acctgcgcga
     1801 tcacgggcgc ggaaagaacc gcgcccgcag cgagcaggct cgctacaagg cttgatccag
     1861 gcatcttcat tcggcttcct tctggacctt ctgacctttg aggaaaagat ccgaccagag
     1921 tttctcccag cggtcggcct cgtcgagcgt gatcaccgga tcgatcatct cgaaggggaa
     1981 cttgttgagg tgggtgtcca ccgcgcggct cgagggcacc cggttgcgct ccttcaggat
     2041 cgtctgtcct tcgagggaga gcatgaagtc ggcaaagagc agcgccgcgt ggggatgggg
     2101 ggcgcggcgc gtcacgccga tcgcgttcgg gcgcccgaag gtgggcttga gcgccttcca
     2161 gcggatcggc gcgcctttga cgaccagccg ctcggcattg tggttgtaga tggtggcggc
     2221 gagcgggatc tcgcccgagg cgacgagctc ggccatcagc gtgtggccgc tgcgcatttc
     2281 gggtttcgca gcggcgagct tcctgaagaa tgcgagaccc ttctcctcgc ccatcgactt
     2341 gaccacggcg ccgaaccagt cgacgtcgct cgcctcgagc ccgactcggc ccgcgaagcg
     2401 tgggttcagc agatcttcgt aggtattggg cacctcgttc gctttgacga gattggtgtt
     2461 gtaggcgatg gtgaagaaat tgaag
//
LOCUS       DASPZD010000207         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1431379, whole genome shotgun sequence.
ACCESSION   DASPZD010000207 DASPZD010000000
VERSION     DASPZD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431379"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1134)
                     /locus_tag="VEM33_04030"
     CDS             complement(154..1134)
                     /locus_tag="VEM33_04030"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYS51022.1"
                     /translation="MSSPPRVVVTGAAGFIGSHLCERLLTLGHRVVGVDSFTDYYERA
                     RKEQNLAGSKANPDFTFEELDLVEANLQPALRGANVVYHLAGQDGVRPRSGGQFDRYV
                     RNNIIATQRLLEALMEMPVDRLVFAGSSSVYGDAEMYPTKESALPRPVSPDGVTKLAA
                     EHMALLYTKNFGLPAVSVRYFTVFGPRQRPDMAFSRFMQALVDREMIEVFGDGEQTRE
                     FTYISDAIEGTIKAATAKVTGQVFNLGGGSRSTINGVLATLEDISGMKVRKHMLPAVP
                     GEPRHTGASINLARERLGWEPRVSLREGLTKQWEWFQAMRDVGARSSGGS"
     gene            1199..1747
                     /locus_tag="VEM33_04035"
     CDS             1199..1747
                     /locus_tag="VEM33_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HYS51023.1"
                     /translation="MNRFEAWLSASGAGIMVKEFPEGTRTAADAARAVGCELGQIVKS
                     LVFVAGGKPVVALMSGANRLDERRLARVAGAPVEKADAEAARAATGFSIGGVPPFGHA
                     SEVPVFMDRDLLGYEEVWAAAGRPDSVFEISPGRLRELSNATVIDLKVEAGSSSPSDA
                     STTPRTKSAGTPKEKRGSHLSK"
     gene            1828..>2105
                     /locus_tag="VEM33_04040"
     CDS             1828..>2105
                     /locus_tag="VEM33_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYS51024.1"
                     /translation="MDRLMPDYVDALGRELARARARYPFEPLETIYLGGGTPSLLPAE
                     LATRLLRLIRANFDVAPGAEVTLEANPASTDERKLAAWLEGGVNRLSLG"
BASE COUNT          361 a          632 c          716 g          396 t
ORIGIN      
        1 cgatctgacc ggacagtatt tcttgctgtt cgtcgcgggc agcaaggcgg ccgtctacat
       61 ctgccggtcg gtcgcggaag gcgaatcggc atcgcgctct tgcgaggcgc ccatcgcgcc
      121 ggttggggcg gcctggaagt cgagcctcag gggctagctg ccaccgctcg accgcgcacc
      181 gacgtcgcgc atcgcctgga accactccca ctgcttcgtc aggccctcgc gcagcgacac
      241 ccgaggttcc cagccgaggc gctcgcgggc gagattgatc gaagcgccag tgtgtcgggg
      301 ttcgccgggg acggccggca gcatgtgctt ccgcaccttc atgccgctga tgtcctcgag
      361 agtcgcgagc accccgttga tcgtgctgcg gctcccgccg cccaggttga agacctgacc
      421 ggtgactttc gcggtcgccg ctttgatcgt gccttcgatc gcgtccgaga tgtaggtgaa
      481 ctcgcgtgtc tgctcaccgt ctccgaacac ttcaatcatt tcgcgatcga cgagcgcctg
      541 catgaatcga gaaaatgcca tgtcgggccg ttgtcgtggc ccaaacacag tgaagtagcg
      601 cacggacacg gcaggcaggc cgaagttttt cgtgtacaga agggccatat gctcagccgc
      661 gagcttggtc acgccgtcgg gggacactgg tcgaggcagc gccgactctt tggtggggta
      721 catctccgca tcgccgtaaa cggatgagct tccggcgaaa acgagccggt cgaccggcat
      781 ctccatcaaa gcctcgagga ggcgctgcgt ggcgatgatg ttgttgcgca cgtagcggtc
      841 gaactgcccg cccgagcgag ggcgcacacc gtcctggccc gccaggtggt agacgacatt
      901 cgcgccgcgg agtgctggct ggagattggc ttcaacaagg tcgagctctt cgaacgtgaa
      961 gtcgggattg gccttcgagc cggcgaggtt ctgctccttc cgggcgcgtt cgtaatagtc
     1021 cgtgaagctg tcgacgccga cgacccggtg tccgagggtg agaagccgtt cgcatagatg
     1081 cgagccgatg aaaccggctg cgccggtcac gacgacacgt ggcggtgagc tcactgccga
     1141 cacagcgtgc ggttcggtgg tgctctattg caactgagaa gcctcctatc ctcataccgt
     1201 gaatcgattc gaggcgtggc tgtcggcgtc gggcgccggc atcatggtca aggaatttcc
     1261 ggaggggacg cgaacggcgg cggatgcggc gcgtgcggtc gggtgcgagc tcgggcagat
     1321 cgtcaagtcg ctggtgttcg tcgcgggtgg caagccggtg gtggctctta tgagtggggc
     1381 gaaccggctg gatgaaaggc ggctcgcgcg ggtcgcaggt gcgccggtcg agaaggccga
     1441 cgccgaagct gcgcgcgccg cgaccgggtt ttcgatcggt ggtgtgccgc cctttggcca
     1501 cgcgagcgag gtcccagtct tcatggaccg cgacctgctc ggttatgagg aggtttgggc
     1561 ggccgcgggg cggccggatt cagtgtttga aatttcgccg ggccggctta gagagctgag
     1621 caatgccact gtgattgatt tgaaggttga ggcgggctcc agctccccat ccgacgcttc
     1681 gaccaccccg cgcaccaaaa gcgcggggac cccaaaggaa aagcggggaa gtcacctatc
     1741 taaatgaagc ctttcaggca tctatatcta catatcccat tctgtaagca caagtgcggg
     1801 tattgcgatt tcaatgccta ctcgggcatg gatcgtttga tgcccgacta cgtcgacgcg
     1861 ctggggcgcg agctggcgcg tgcccgggcg agatacccgt tcgaaccgct cgagaccatt
     1921 tacctcggcg gcgggacgcc gagcctgctg cccgcggagc tcgcgacgcg gctcttgcga
     1981 ctcattcgcg caaatttcga tgtggcgccc ggagccgagg tcacgttgga agcgaacccc
     2041 gcgagcaccg acgagcgcaa gctcgctgct tggctcgagg gtggagtaaa tcgcttgagc
     2101 ctcgg
//
LOCUS       DASPZD010000208         4170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1622967, whole genome shotgun sequence.
ACCESSION   DASPZD010000208 DASPZD010000000
VERSION     DASPZD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622967"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="glmM"
                     /locus_tag="VEM33_04045"
     CDS             <1..892
                     /gene="glmM"
                     /locus_tag="VEM33_04045"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447373.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYS51025.1"
                     /translation="RRVEDAQGRYIEFCKSTFPSSLDLRGMKIVIDCAHGATYHVAPH
                     VFHELGADVETIGANPDGFNINDRAGTVHPGILQGAIAEHKADLGIALDGDGDRVLMA
                     DGDGRLYDGDELLFVIARERSKNGGLKGVAGTLMTNLALERALEEMGISFARAKVGDR
                     YVLEMLQEKDWQLGGENSGHIICLDKHTTGDGIVSALQVLSAVRTRRATLGTLCQGLA
                     LYPQELVNVRVAKGFDWQKQPAIAKAKSEAERALGKDGRVLLRPSGTEPVLRVMVEGK
                     DDAEVRRLARMIAETVQEAA"
     gene            1044..1799
                     /gene="tpiA"
                     /locus_tag="VEM33_04050"
     CDS             1044..1799
                     /gene="tpiA"
                     /locus_tag="VEM33_04050"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYS51026.1"
                     /translation="MRARLVAGNWKMHGSLAANARLLEALKAGLPEAEGLGYAVCAPY
                     PYLAQVSQALSGSRIAWGAQNVSEHDSGAYTGEVSGAMLKEFRCRFAIVGHSERRGLY
                     GEDDGRVAAKFLAARRAGLTPILCVGETLEQRENGETENVVGRQLGAALEAAGVAALA
                     DAVLAYEPVWAIGTGKNATPQQAQYVHAFIRARLAKRDPSLAQRVTILYGGSVKPGNA
                     GELFAMPDVDGGLIGGASLVAEDFIAICAAARR"
     gene            1816..2199
                     /gene="secG"
                     /locus_tag="VEM33_04055"
     CDS             1816..2199
                     /gene="secG"
                     /locus_tag="VEM33_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HYS51027.1"
                     /translation="MDFWFTLILIVDVIVALCIIGLVLLQHGKGADVGAAFGSGASGS
                     LFGASGSANFLSRTTAVLAVVFFLTTFVLAYLVTHKPRVGGGVMDTVKERPQPAQQQV
                     PAPQPTPDAAKASDAAKSKANEVPK"
     gene            2261..2345
                     /locus_tag="VEM33_04060"
     tRNA            2261..2345
                     /locus_tag="VEM33_04060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2295..2297,aa:Leu,seq:gag)
     gene            2486..2842
                     /locus_tag="VEM33_04065"
     CDS             2486..2842
                     /locus_tag="VEM33_04065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYS51028.1"
                     /translation="MLENYFPILLFIVIAFVFGGIMVAAGTVLGPHRPDSEKLSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVTLNEIGALGFWSMMMFLAIL
                     VVGFVYEWMKGALEWE"
     gene            2861..3337
                     /locus_tag="VEM33_04070"
     CDS             2861..3337
                     /locus_tag="VEM33_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HYS51029.1"
                     /translation="MGIEGVFEEGFATTSLDKLINWTRTGSLWPMTFGLACCAVEMMH
                     AGAARYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIVQLQNKIKRTNTIAR"
     gene            3353..3961
                     /locus_tag="VEM33_04075"
     CDS             3353..3961
                     /locus_tag="VEM33_04075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYS51030.1"
                     /translation="MAAKLGRLKDALDSSLAGKIVSLSESLGELTLVVDAKDYSATAQ
                     WLREDPAVRFEELIDLCGVDYSGFRDGRWGGRRFAVVSHLLSLVHNWRLRLRVFAPDD
                     DFPVVDSLTGVWPNADWYEREAFDLFGIMFTGHADLRRILTDYGFIGHPFRKDFPLSG
                     NVEMRYDPEQKRVIYQPVTIEPREITPRVIREDEYGDLGTKH"
     gene            3965..>4170
                     /locus_tag="VEM33_04080"
     CDS             3965..>4170
                     /locus_tag="VEM33_04080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901973.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYS51031.1"
                     /translation="MAEIRNYTLNFGPQHPAAHGVLRLVLELDGEVIMRADPHIGLLH
                     RATEKLAEHKTYIQALPYMDRLDYV"
BASE COUNT          762 a         1284 c         1376 g          748 t
ORIGIN      
        1 tcggcgcgtc gaggacgccc agggccgcta catcgagttc tgcaagagca cgtttccgtc
       61 ctcgctcgac cttcgcggga tgaagatcgt gatcgactgc gcgcacggag cgacctatca
      121 cgtcgctccc cacgttttcc acgagctggg cgccgacgtc gagacgatag gagcgaatcc
      181 cgacgggttc aacatcaacg atcgcgccgg aaccgtgcat ccggggatcc tgcagggcgc
      241 gatcgccgag cacaaagccg atctgggcat cgcgctcgat ggcgacggcg accgcgtgct
      301 tatggccgac ggagatggaa ggctttacga cggtgacgag ctcctgttcg tcatcgcacg
      361 cgaacgctca aaaaacggcg ggctgaaagg cgttgccgga accttgatga ccaatctcgc
      421 gctggagcgc gcgctggaag aaatggggat ttccttcgca cgcgccaaag tcggggaccg
      481 ctacgtgctg gagatgctcc aggaaaagga ttggcagctc ggaggtgaga attccgggca
      541 catcatctgc ctggacaagc acacgaccgg cgacggcatc gtgtcggcct tacaggtgct
      601 ctccgcggtg cgcacgcgcc gcgcgactct cgggaccctg tgccagggac ttgcgctcta
      661 tccgcaggag ctcgtcaacg tgcgcgtggc caagggcttc gactggcaga aacagccggc
      721 gatcgcaaag gcgaagagcg aggccgaacg cgccctcggc aaggacgggc gggtgctgtt
      781 gcggccgtcg ggcaccgagc ccgtgctccg cgtcatggtg gaaggcaagg acgacgcgga
      841 agtccggcgg cttgccagaa tgatcgccga aaccgtgcaa gaggcggcct aatctcgccg
      901 gatgatcgcg ctgctgcagc gcgtctcgcc ggctcgaggt cctgacgccg gaacataaga
      961 tcctgaattt tccagcgttt tcggttgacc ctcacagcgg tccggcagta aaatctcccc
     1021 ctttttggct ctcccggaac gggatgcgcg cgaggctcgt agcaggcaac tggaagatgc
     1081 acggcagcct cgcagcgaac gcacggctgc tcgaggcgct caaggcgggc cttccggaag
     1141 ccgaaggcct cggctacgcg gtgtgcgcac cgtatccgta cctcgcacaa gtctcgcagg
     1201 ccctgtcggg cagccggata gcctggggcg cgcagaacgt ttccgaacac gactccgggg
     1261 cctacactgg ggaggtgtcg ggggcgatgc tcaaggagtt ccgctgccgt ttcgcgatcg
     1321 tcggccattc ggagcggcgc ggactctacg gggaagacga tggcagagtc gccgccaaat
     1381 tccttgcggc acgccgcgcc ggcctgacgc cgattctgtg cgtgggtgag accctggagc
     1441 agcgcgagaa cggcgagact gaaaacgtcg tggggcgtca actcggcgcg gcgctcgagg
     1501 ccgccggggt cgccgctctg gcagacgccg tgctcgccta cgaaccggtc tgggcgatcg
     1561 gcacgggtaa aaatgccacg ccgcagcagg ctcagtacgt gcacgcgttt atcagggcac
     1621 ggctcgcgaa gcgcgacccg agcctcgcgc agcgcgtgac gatcctgtac ggaggcagcg
     1681 tcaaacccgg aaacgccgga gagctgtttg caatgccgga cgtcgacggc ggattgatcg
     1741 gcggggcttc gctcgtcgcg gaggacttca tcgcgatctg cgctgcggcg cggagataac
     1801 tctggagaat gcaatatgga tttctggttc acgctgatcc tcatcgtcga cgtcattgtc
     1861 gcgctctgca tcatcggtct cgtcctgctc cagcacggca agggagccga cgtaggcgcg
     1921 gctttcggca gcggcgcctc gggaagcctg ttcggcgcaa gcggctcggc caattttctg
     1981 tctcgcacga cggccgtcct cgcggtcgtg ttcttcctca ctacttttgt gctcgcctac
     2041 ctcgtcacgc acaagccgcg tgtgggagga ggcgtcatgg acacggtcaa ggaacggccg
     2101 cagccggcgc agcagcaggt gcccgcgcca cagcctacgc ccgacgctgc aaaggcgtcc
     2161 gacgcggcga aatcgaaagc aaacgaggtg ccgaagtagt aaaatcggac gctttgaggg
     2221 cgggctcgtt tctgacacga cgacgcttcc agcccatcac gccgacgtgg tgaaattggt
     2281 agacacgccg tcttgagggg gcggtggcga aagctgtgag agttcgagtc tctccgtcgg
     2341 caccagacaa agggggactt ggatcggaag caggggactt ccggcccaag tcctctattt
     2401 caagaggggt atggtttctg tgagagcgag cgtgcgagtg cgagccaccg gcacggcatc
     2461 ctgctctcag gcccgcggct gacccgtgct cgaaaactat ttcccgatcc tgctgttcat
     2521 cgtgatcgct tttgtcttcg gcggcatcat ggtcgccgcg ggcacggtgc tcggaccgca
     2581 ccgtcccgac agcgaaaagc tgtccccgta cgaatgcgga ttcgaggcct tcgaggacgc
     2641 gcgcatgaaa ttcgacgtgc gttactacct ggtcgcgatc ctgttcatcc tgttcgacct
     2701 ggaaatcgcg tttctcttcc cctgggcggt gacgctcaac gagatcggcg ccctcggctt
     2761 ctggtcgatg atgatgttcc tcgccattct cgtcgtgggc ttcgtatacg aatggatgaa
     2821 gggagcgttg gagtgggagt agtgaaagtg aaggtggtcc atgggaattg aaggcgtatt
     2881 cgaggaagga ttcgcgacga cctcgctcga caagctcatc aactggacgc gcaccggatc
     2941 gctgtggccg atgacgttcg gcctggcgtg ctgtgccgtc gagatgatgc acgcgggcgc
     3001 cgcgcgctac gatctcgatc gcttcggaat cgtatttcgc cccagcccga gacagtccga
     3061 cgtcatgatc gtcgcgggga ctctgtgcaa caagatggcg ccggcgcttc gcaaggtgta
     3121 cgaccagatg gccgaaccgc gctgggtgat ctcgatgggg tcctgcgcga acggcggcgg
     3181 ctactaccat tactcgtact cggtggtgcg cggctgcgac cgcatcgtgc cggtcgacgt
     3241 gtacgtgcct ggctgcccgc cgactgcgga ggccttgctc tacggcatcg tccagctgca
     3301 gaacaagatc aagcgcacca acaccatcgc gcgctgattg gaccgaaggc atatggcggc
     3361 gaagctcgga cgactgaaag acgcgctcga ctcgtctctc gccgggaaga tcgtgagcct
     3421 gagcgaatcc ctgggcgagc tgacgctcgt ggtcgatgcg aaagactatt ccgcgacggc
     3481 gcaatggctg cgcgaggacc cggctgtgcg cttcgaggag ttgatcgacc tgtgcggagt
     3541 ggattactcg ggcttccgcg acggtcgctg gggaggacgc cgtttcgcgg tcgtgtcgca
     3601 cttgctttcg ctggtgcaca actggcggct gcgcctgcgc gtgttcgctc cggatgacga
     3661 cttccccgtg gtcgattccc tcaccggcgt gtggcccaac gccgactggt acgagcgcga
     3721 ggctttcgac ctgttcggaa tcatgttcac cgggcacgcc gatctgcggc gcatcctcac
     3781 cgactacggc tttatcggcc atccgttccg taaggacttt ccgctgtcgg gaaacgtcga
     3841 gatgcgatac gacccggagc agaaacgcgt catctatcag ccggtgacga tcgaaccccg
     3901 cgagatcacg ccgcgcgtca ttcgcgagga cgagtacggc gacctgggaa ccaagcactg
     3961 acacatggcc gagatccgga actacacgct gaacttcggc ccgcagcacc ccgcggccca
     4021 cggcgtgttg cgcctggtgc tggagctgga cggcgaggtg attatgcgcg ccgatccgca
     4081 catcggcttg ctgcaccgcg cgaccgagaa gctcgcggag cacaagacct acatccaggc
     4141 gctaccctac atggaccggc tcgactacgt
//
LOCUS       DASPZD010000209         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1597600, whole genome shotgun sequence.
ACCESSION   DASPZD010000209 DASPZD010000000
VERSION     DASPZD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597600"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VEM33_04085"
     CDS             <1..670
                     /locus_tag="VEM33_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYS51032.1"
                     /translation="EARTRERNVEIAAIESARRTERAALVRQQAEHRKVLARVSSQLS
                     AQRREVQQLERDESRLSRLVEELGRMIASTPLLRNERVPEPGGAEESLSGLKRGLRLP
                     IRGVLANRFGAQRPSGGPSWKGLFIRSPSGQEVRAVGSGRVVFADWLRGFGNLLIIDH
                     GKGYLTIYGNNESVLKQVGEPVRTGDAVATVGASGGNMESGLYFEIRHEGKAFDPMRW
                     VSLK"
     gene            718..2124
                     /locus_tag="VEM33_04090"
     CDS             718..2124
                     /locus_tag="VEM33_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYS51033.1"
                     /translation="MMIQIRRASLVLVGVLAGVLLSLNFSAVAQREAARFPLPVDELR
                     AFAEVFGAIKSSYVEPVEDKKLITEAISGMLTGLDPHSAYLDQDAFRELQVGTQGQFG
                     GLGIEVGMEEGFVKVVSPIEDTPAFRAGLKPGDFIIKIDDTSVKGMTLNDAVKRMRGK
                     PKTTIRLTILRKGEPKPFEVTLTRDIIRVQSVKSKVIEPGYGWVRVSQFQEHTGENLV
                     KHIEGLYKNGPLKGLILDLRNDPGGLLNGAVGVSAAFLPRGVPVVSTDGRTEDAKRKF
                     IAAPEDYLRGNREDYLKNLPAAVKTVPMVVLVNGGSASASEIVAGALQDHKRATVVGT
                     ATFGKGSVQTILPLGNNTAIKLTTARYYTPSGRSIQAKGIVPDVAVEDPTAPTTGRLR
                     EADLEKHLLNDQESEVQSEKAQRTSAKPATDAEEKPPAAPLEFASEKDFQFQQALKLL
                     KGLPLATAQSAPATPAKN"
BASE COUNT          458 a          711 c          732 g          350 t
ORIGIN      
        1 cgaggcgcgg acccgggaaa ggaacgtcga gatcgccgcc atcgaatcag cgcggcgcac
       61 ggagcgcgcc gcgctggtca gacagcaggc ggagcaccgc aaagtgcttg cgcgcgtttc
      121 cagccagctg agcgcgcagc gccgcgaggt gcagcagctc gagcgcgacg aatcccgcct
      181 gtcgcggctc gtcgaggagc tcggcagaat gatcgcgtcc acgccgctcc tgcgcaacga
      241 aagagtcccc gagcccggag gtgcggaaga gtccctctcg gggctcaagc gcggactgcg
      301 gctgccgatc aggggtgtgc tcgcaaaccg cttcggcgcc cagcgtccca gcggcgggcc
      361 ctcgtggaag ggtctgttca ttcgctcgcc ttcggggcag gaagtgcgcg ccgtcggatc
      421 ggggcgggtc gtgttcgcgg attggctgcg cggcttcgga aacctgctca tcatcgatca
      481 tggcaaaggc tatctcacca tctacggcaa caacgaatcg gtgctcaagc aggtcggcga
      541 gccggtccgc acgggcgacg cggtcgcgac cgtcggggcg agcggcggca acatggaatc
      601 gggtctatac ttcgagattc gacacgaagg aaaagccttt gatccgatgc gttgggtgtc
      661 tttgaagtga ccgataacga cgccaagcag ccgcctccgg gcccgggacc gaatatcatg
      721 atgatccaga tcagacgggc cagccttgtg ctggtcgggg tgctggcagg agtcctgctc
      781 agcctgaact tctccgcggt ggctcagcgc gaagcggcgc gcttcccgct tccggtggac
      841 gagctgcgcg cctttgccga agtgttcggc gcgatcaagt cgagctacgt cgagccggta
      901 gaggacaaaa agctcatcac ggaagcgatc agcggcatgc tgaccggact cgatccgcac
      961 tcggcttatc tcgaccagga cgcgttccgc gagcttcagg tgggcaccca gggccaattc
     1021 ggcggcctcg gcatcgaggt cggaatggaa gaagggttcg tcaaggtggt gtctcccatc
     1081 gaggatacgc cggcatttcg cgccggcctg aaacccgggg atttcatcat caagatcgac
     1141 gacacctcgg tcaagggcat gaccctgaac gacgcggtca aacgcatgcg cggcaaaccg
     1201 aaaacgacca tacggctcac catcctgagg aaaggcgagc ccaagccctt cgaggtcacc
     1261 cttacccgcg acatcattcg cgtgcagagc gtgaagtcca aggtgatcga acccggctac
     1321 ggctgggttc gcgtctcgca gttccaggag cacaccgggg aaaatctcgt caagcacatc
     1381 gaggggctgt acaagaacgg gccgctgaag ggcctgattc tggatttacg caacgatccg
     1441 ggtggactgc tgaacggcgc ggtaggtgtc tccgcggcgt tcctgccgcg tggggtgccg
     1501 gtggtgtcga ccgacggccg caccgaagac gccaagcgca aattcatcgc cgcacccgag
     1561 gattatcttc gcggcaaccg cgaggactat ctgaaaaacc tgcccgcggc ggtgaaaacc
     1621 gtgccgatgg tggtgctggt caacggcgga tcggcctccg cgtccgaaat cgtcgcgggc
     1681 gcgctgcaag accacaagcg cgcgacggtg gtgggcacgg cgacattcgg caagggctcg
     1741 gtgcagacga ttctgccgct gggcaacaat accgcgatca aactgaccac cgcccgctac
     1801 tacaccccga gcggtcgctc gatccaggcc aaaggcatcg tgcccgacgt cgctgtcgaa
     1861 gatcccacgg cgcccaccac gggacgcttg cgcgaagccg atctggaaaa gcatctcctc
     1921 aacgaccagg agagtgaagt ccagtcggag aaagcgcaac ggaccagcgc aaaaccggcg
     1981 acggatgccg aagaaaaacc gccggcggcg ccgctcgagt tcgcctcgga aaaggacttc
     2041 cagttccagc aggcgctcaa gctcctgaag ggcctgccgc tcgccaccgc gcaaagcgca
     2101 ccggccacgc ccgcgaagaa ttgatcaagg cggacgcctt tggcgccgct tgatctccgc
     2161 gggatggaac cgacgcggag cgcggttcat cccgcggtca acgattgtcc tctatgaatg
     2221 acgatcaact cctacggtat tcgcgccaca t
//
LOCUS       DASPZD010000210         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1098794, whole genome shotgun sequence.
ACCESSION   DASPZD010000210 DASPZD010000000
VERSION     DASPZD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098794"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VEM33_04095"
     CDS             complement(<1..1018)
                     /locus_tag="VEM33_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS51034.1"
                     /translation="MASRVRSPGRTAFEAGVEAYKAGNLAAAAGHFADTLRAEPEHVQ
                     AHNCAGGIDLRTGRFREALKHFERGRMLDPQNAECHFGAAVAHRELGANEAARECCEK
                     ALALDPGLHPAHGFLASLNLPGPFYLEVISMFHSHLRPRTYLEIGVETGVSIALARRE
                     TRAIGIDPEPKITERLAPGTSIHATTSDEYFAAHDVRAEFGGLPIDLAFIDGMHWFEF
                     ALRDFMNIERHCTSRSTILIHDCYPLSRHTAERERRTSFWSGDIWRLILLLRKYRPDL
                     SVKVIGTAPTGLGVVRGLDPGSNVLREKYDAIVGEFLALDYSVLEADKPGMLALYPND
                     WEKLK"
     gene            1139..>2350
                     /gene="alaS"
                     /locus_tag="VEM33_04100"
     CDS             1139..>2350
                     /gene="alaS"
                     /locus_tag="VEM33_04100"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYS51035.1"
                     /translation="MKSSEIRAKFLKFFEERGHAVVASSPLVPANDPTLLFTNSGMVQ
                     FKDVFLGKETRVYKRATTAQRCLRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     RDAIRYAWDLLTKVYKLPPERLWTTVYHEDDEAYDLWTKEIGVPKGRCIRIGDKPGSP
                     KYQSDNFWQMADTGPCGPCSEIFYDHGPGIPGGPPGSSEAEGDRYIEIWNLVFMQFNR
                     DDKGAVTPLPKPCVDTGMGLERIAAVLQGVHSNYEIDLFRDLIRAAGRETGTKDLVNN
                     SLNVIADHIRACAFLIVDGVIPGNEGRGYVLRRIIRRAIRHGYKLGRKEPFFHRLVAD
                     LSRAMGEAYPELLRAKERVTQVLKAEEERFAETLENGMKVLEGALHREDRMLDGETVF
                     QLYDTFGFPVDL"
BASE COUNT          455 a          738 c          743 g          414 t
ORIGIN      
        1 ccttcaactt ctcccagtcg ttgggataga gggcgagcat tcccggcttg tcggcttcga
       61 gcaccgaata gtcgagcgcc agaaactccc cgacgatcgc atcgtacttc tctcgcagga
      121 cgttcgaacc ggggtcgaga ccgcgcacca cgcccaggcc ggtcggcgcc gtcccgatca
      181 ctttcacgct caggtcggga cgatatttcc tgaggaggag gatgagccgc cagatgtcgc
      241 cgctccagaa gctggttcgg cgctctcgtt cggcggtgtg gcgcgacaag gggtaacagt
      301 cgtgaatcaa tatcgtcgat cgtgacgtac agtgccgctc gatgttcatg aagtcgcgca
      361 gtgcgaattc gaaccagtgc atcccgtcga tgaaagcgag atcgataggc aagccgccga
      421 attccgcccg cacgtcgtga gcggcgaagt actcgtcgct cgtggtggca tgtatgctcg
      481 tgccgggagc gagccgctcc gtgatcttgg gttcggggtc gatgccgatc gcgcgcgttt
      541 cccgccgggc gagcgcgatc gacaccccgg tctcgacgcc gatctccagg taagtgcgcg
      601 gacgcaaatg cgaatggaac atcgagatga cctcgagata gaagggtccg ggaaggttga
      661 ggctcgcaag gaagccgtga gcggggtgca gcccggggtc gagcgcaagc gctttttcgc
      721 agcattcgcg cgcggcctcg ttcgcgccca gctcccggtg cgccacggcc gcgccgaagt
      781 ggcactcggc attttgcggg tcgagcatgc ggccacgttc gaaatgcttg agcgcctcgc
      841 ggaaacgtcc cgtccgcaaa tcgatcccgc cggcgcagtt gtgcgcctga acgtgttcgg
      901 gctccgcgcg cagcgtgtcg gcaaaatgcc ctgctgcggc agcaaggttg cccgccttgt
      961 acgcttccac cccggcttcg aaggccgtcc gcccgggcga gcggactctc gacgccaata
     1021 gatctttcag tatccgtccc agcatgcgcg ccaagcttaa ctcaatacgg aagagtcgtt
     1081 gcacgagccg atgaatcggg cgaaaatcga gcgcttcaag gagttgtccc aggaattcat
     1141 gaaatcgtcc gaaatcaggg caaaatttct gaagttcttc gaagagcgcg gccacgcggt
     1201 cgtcgcctcg agcccgctcg tgccggcgaa cgatcccacg ctgcttttca ccaactcggg
     1261 catggtgcag ttcaaggacg tatttctcgg caaggaaacc cgggtgtaca agcgcgccac
     1321 gaccgcacag cgctgcctgc gcgcgggcgg aaaacacaac gatctggaga acgtcggcta
     1381 cactgcgcgc caccacacgt tcttcgagat gctcggcaat ttttcattcg gcgattactt
     1441 caagcgcgac gcgatccgct atgcctggga cctgctgacc aaggtgtaca agctcccgcc
     1501 ggagcgcctg tggacgacgg tgtatcacga ggacgacgaa gcctacgatc tctggacgaa
     1561 ggagatcggc gtgccgaaag ggcgctgcat ccgcatcggc gacaagcccg gaagcccgaa
     1621 ataccagagc gataacttct ggcagatggc cgacacgggc ccctgtggtc cctgcagcga
     1681 gattttctac gaccacgggc ccgggatccc gggcggcccg cccggctcga gcgaagccga
     1741 aggtgatcgc tacatcgaga tctggaatct cgttttcatg cagttcaatc gggacgacaa
     1801 gggcgcggtg acgcccctgc ccaaaccctg cgtggacacg ggcatgggcc tggagcggat
     1861 cgcagccgtt ctccagggcg tgcactcgaa ctacgagatc gacctgttcc gggacctgat
     1921 acgcgccgcg gggcgggaga cgggaacgaa ggacctcgtg aacaattcgc tgaacgtgat
     1981 tgccgaccac atccgcgcgt gcgcctttct catcgtggac ggcgtcatac ccggaaacga
     2041 agggcgcggt tacgtgcttc gccgcatcat ccgccgcgcc atccgccacg gctacaagct
     2101 cgggcgcaag gagcctttct tccaccgcct cgtcgcggat ctgtcacgcg cgatgggcga
     2161 ggcctatccg gagctcctgc gggcgaagga gcgcgtcacc caggtcctca aggccgagga
     2221 ggagcgtttc gccgagacgc tggagaacgg catgaaagtg ctcgagggcg cgctgcatcg
     2281 cgaagaccgc atgctcgacg gcgaaaccgt gttccagctc tacgacacct tcggctttcc
     2341 ggtcgatctg
//
LOCUS       DASPZD010000211         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_922807, whole genome shotgun sequence.
ACCESSION   DASPZD010000211 DASPZD010000000
VERSION     DASPZD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922807"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1353)
                     /locus_tag="VEM33_04105"
     CDS             complement(184..1353)
                     /locus_tag="VEM33_04105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS51036.1"
                     /translation="MSLFLNLGHTLDHLLMLIFPTVVLAMGAELGRGYAELLPLSLGG
                     FVAFGAFSIPAGWLADRWSRRGMIVLFFFGIGTASILTGFATGPLHIALGLTLVGVFA
                     AIYHPVGIAMLVANRENVGRVLGVNGVFGNVGVAFSALIAGALADTVGWRAAFIVPGL
                     IALGVGTAFVLFAREAQGETPLRRSGFYRASRADLARVFAVLTVATACGGVIFNAATI
                     SMPKVFDERLSSLTHSTLGIGMLVCGVYLTAAVAQLCVGWWLDRRPLKSVFVPVVALQ
                     VPLLALAGTTESYLMLATAVAMMFFVFGQIPINDAMIARYTAEEWRARAYAVRYVVSF
                     GASALAVPLVAWVYKSSGDFKLLYYVLGTLAFVTFIAALLFPAEEEKAAAKEMAA"
     gene            1486..2526
                     /locus_tag="VEM33_04110"
     CDS             1486..2526
                     /locus_tag="VEM33_04110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HYS51037.1"
                     /translation="MTTRSILSALTAFIFVLGGAVAQEPKEAPHAQKSQQTQADLERE
                     AEEMLGAVVRVRMKAIPNARSNTTLGPSREGTGVVIDERGHIVTIGYIVIEADSIEIT
                     TRDERTVPAALIGYDHASGLGLLRSGAPLGVKPMPMGQAADLAAREPVMILPAGGREA
                     ANLAYVVSKRKFAASWEYLLDTAIFTAPPTSQWAGAALVSREGKLVGIGSLYVRETQE
                     RGSEIPGNMFVPIDLLKPILADLIEKGRRSGPARPWLGLATEELHGHLFVTRVSPEGP
                     ADKAGVRNGDIVVGVGADAVKSHEELYRRVWGLGAAGVEVPLKILQGADLRELRVRSI
                     DRFQYFREKPIY"
     gene            complement(2604..2780)
                     /locus_tag="VEM33_04115"
     CDS             complement(2604..2780)
                     /locus_tag="VEM33_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51038.1"
                     /translation="MEIAIVALQTLCFAGLALGTALSMYQLMQRDKVTDPFRYAVAND
                     FETGFRRLVRNRRR"
BASE COUNT          620 a          913 c         1020 g          485 t
ORIGIN      
        1 cgcagcttcg ccctctcgac gagaactttg gcgatagcct gccccgcaga gttggagaag
       61 gaccccgccg gtgtggtgac gattccgatc gtctgggcgg ctgctgcgcc cgtcagcaag
      121 ggcgcacaca gggcaacggc gcgcagcgat ttcatggtcg acccctcctc gtatgctggc
      181 gggctaggcc gccatttctt ttgcggcggc tttctcttcc tcggcgggga acagcaatgc
      241 tgcgataaaa gtgacgaacg ccagcgttcc cagcacgtag tacaggagct tgaagtcccc
      301 gctcgatttg tacacccagg cgacgagcgg cacggcgagc gcgctcgctc cgaacgagac
      361 cacatagcgc accgcatacg cgcgcgcgcg ccattcttct gcggtgtagc gggcgatcat
      421 ggcgtcgttg atcgggatct gcccgaacac gaagaacatc atcgctacgg cagtcgcgag
      481 catcaggtag ctttcggtcg tgccggcgag agcaaggagc ggcacctgca gcgcgaccac
      541 cgggacaaac accgatttga gcgggcggcg atcgagccac cagccgacgc acagctgcgc
      601 caccgcagcg gtgaggtaga cgccgcacac gagcatgccg atacccaagg tcgagtgggt
      661 caggctcgag aggcgctcgt cgaacacctt gggcatggaa atggtcgctg cattgaagat
      721 caccccgccg caagccgtgg cgacggtgag gaccgcgaac acgcgggcga ggtctgcgcg
      781 cgaggccctg tagaacccgc tccggcgcag cggcgtttca ccttgcgctt cgcgtgcgaa
      841 cagcacgaat gccgtgccca cgccaagagc gattaacccc ggaacgatga acgccgcccg
      901 ccagccgacg gtgtcggcga gggcgcccgc gatcagcgcg gagaacgcga cgccgacgtt
      961 gccgaaaact ccgttcaccc cgaggacccg gcctacgttc tcccggttcg cgacgagcat
     1021 cgcgattccc accgggtggt agatcgcggc gaacactccg acgagcgtga ggcccaaggc
     1081 gatgtgcagc gggccggtgg cgaagcccgt gaggatgctc gcggtgccga tgccgaagaa
     1141 gaacagcact atcatccctc gccggctcca gcggtcggcg agccagcccg cggggatcga
     1201 aaaagcgcca aaggcgacga agccgccgag cgagagcggc aggagttccg cgtaaccgcg
     1261 gccgagctcg gcgcccatcg cgagcaccac ggtggggaaa atgagcatga ggagatgatc
     1321 gagcgtgtgc ccgaggttga ggaacaagct catgcgcgag ttcatcggga gatgataacc
     1381 gggcggaccg gacgccgctc ggctggagga gagtctggtg ttgccaagtg gaaatcagcg
     1441 ccgtttagta tgacaattga taaagcgacc tcggggagca tcacgatgac tacaagaagc
     1501 atcctttccg ccttgaccgc cttcattttc gtgctggggg gcgcggttgc tcaggaaccg
     1561 aaggaagcac ctcacgccca gaaatcccaa cagacccaag cggatctcga aagagaagcg
     1621 gaggaaatgc ttggcgccgt ggtgcgggtc aggatgaagg ccatccccaa tgcccgcagc
     1681 aacacgaccc tgggtccgag ccgcgagggg accggcgtcg tgatcgatga gcgcggacac
     1741 atcgtcacga tcggttatat cgtgatcgag gccgattcga tcgagatcac cacgcgggac
     1801 gagaggaccg ttcccgcagc gttgatcggc tacgaccacg cgagcggcct cggactgctg
     1861 cgctcgggcg cgccgctggg ggtgaagccc atgcccatgg ggcaggctgc cgacctcgcc
     1921 gcgcgcgagc cggtcatgat cctcccggcg ggcggacgcg aggcggcgaa tctcgcttac
     1981 gtggtctcca agcgcaaatt cgcggcgagc tgggaatacc tgctcgacac cgcgatcttc
     2041 acggcgccgc cgacctcgca gtgggccggc gcggcgctgg tgagccgcga ggggaaactg
     2101 gtcggcatcg gctcccttta cgtgcgcgaa acgcaagagc gcggatcgga gatcccggga
     2161 aacatgttcg tgccgatcga cctgctgaaa ccgatcctcg ccgacctcat cgaaaaagga
     2221 cgcaggagcg gtcctgcgcg tccgtggctg ggtctcgcca ccgaggagct gcacgggcac
     2281 cttttcgtga cgcgggtatc gccggagggt cctgcggaca aggccggggt caggaacgga
     2341 gatatcgtcg taggcgtcgg cgcggacgcg gtcaagagcc acgaggagct ctaccgcagg
     2401 gtgtggggcc tgggcgcggc cggggtcgaa gttccgctga agatcctgca gggcgcggac
     2461 ctgcgcgagt tgagggtgcg ctcgatcgac cgcttccagt acttcaggga aaagccgatt
     2521 tactgaatca ggaggagcgg tccaaaaaaa atcggcgtag ccgcgacgcg ggtacgccga
     2581 aaggtgctac ggaggagatc gttctaccgg cggcggttgc gcacgaggcg gcgaaagccg
     2641 gtttcgaaat cgttggcgac tgcgtacctg aaagggtcgg taaccttgtc gcgttgcatc
     2701 agctggtaca tggacagcgc ggtccccaga gcaagacctg caaaacacag ggtctgaagt
     2761 gcgacgattg cgatttccat ggtctgtctc ctacttccgg cgagcagttc gtatccttgt
     2821 cctacctgga gcaggaagcg ggccaggctg attcgcagac cttagggctg gagaattaat
     2881 acgtaagtta atgatttata tttattatta gtacatacca cgattatggc atctgaatgt
     2941 gagcgcacac tcatagtgat gctcggccga caacgacgtc acggctgtcc gaggggaaag
     3001 ggccccttcg cttgagcggg cagttcaccg gcacgatg
//
LOCUS       DASPZD010000212         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1732002, whole genome shotgun sequence.
ACCESSION   DASPZD010000212 DASPZD010000000
VERSION     DASPZD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732002"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VEM33_04120"
     CDS             <1..211
                     /locus_tag="VEM33_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYS51039.1"
                     /translation="EPKFVEDKPSRVTYEIEPMGLVCKLTVTHDQFPRGGPTFEAVKE
                     GWPEILSSLKSLLETGEALAIPRNA"
     gene            285..620
                     /locus_tag="VEM33_04125"
     CDS             285..620
                     /locus_tag="VEM33_04125"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS51040.1"
                     /translation="MEAVKDASHLYEVERRATHAERPGFRINEIQISPKQRVPWHYHN
                     NVQDAFYVLQGKIRIFLRDPKEEVRLGPGETFSVRPKRPHLVINGGDASATFLVLQGI
                     GEYDFVPLT"
     gene            666..1907
                     /locus_tag="VEM33_04130"
     CDS             666..1907
                     /locus_tag="VEM33_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYS51041.1"
                     /translation="MDTIDRSLSEEQRMMRASCRAFVNDFVTPFIRQNWQREWLMDPD
                     ARLPRAILEQADRIGIRTLGVPEEFGGVPLDPAHEVQTFALISEEIARGDSGLSDKLV
                     QIWKVSVLLRNVAPRHLQQLWFPRIVKDPGFLLAHCLTEPRGASDRWLPYNAPEAAMQ
                     TRAVLKGDEWVLNGRKQFISNGYDASLYVVYANTRPGAGMLQGTSSFLVPRGTPGLTI
                     ARCNETLGGRFMNNGELVFEDMRLPRDHLLVENDALAKTGVYFKPGKIIQASKNLGVG
                     VAAFERTAEYVQNYVQGGRILIKHQAVALRLAEMATKLRAVRALLHEAARAVDERAPD
                     AEELCNMVKLFASEEVFKVAQHAVELHGGNGIMLDFGIEKLFRDAAVFLHMDATVDIS
                     KFKIVKLMFPHTAGAYAGPEA"
     gene            1916..2890
                     /locus_tag="VEM33_04135"
     CDS             1916..2890
                     /locus_tag="VEM33_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51042.1"
                     /translation="MHLRQACLLTLGTALLALTSQVFAQEPYPSRTIRIIVPFTPGSV
                     TDVMGRSVSDKLAANLGQSVIVENRPGAGGTIGIAQVAKSAPDGYTLVVVSAGYAVNP
                     VIYENLPYDSAKDLAGVIPLGNLPSVLFVSPALGVKSVQELIALAKAKPGTLNYPSAG
                     IGSASHVNAEKFLAVTGIKAVHVPLKGAPEMITETMAGRTQFGTVGISAAQSAIRDGR
                     LIALAVSGKKRAPNLPEVPTVAEAGVPGAEFDFWIGVLAPGQTPRPIIHKLHAEFDRA
                     LQSPDVRDRHAKLGADPMPMSPEQFDAQMREDLATLGALLRAAGVKAN"
     gene            2955..3248
                     /locus_tag="VEM33_04140"
     CDS             2955..3248
                     /locus_tag="VEM33_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51043.1"
                     /translation="MAAKREEYIAKLKTQLDQWNAEMAKWESQAAQAQASMRAEYEKQ
                     LAAIRQHQDQAREQLRKVQAASGDAWMELSRGADEAWAAMRKTFEKASAQFKK"
     gene            complement(3283..3939)
                     /locus_tag="VEM33_04145"
     CDS             complement(3283..3939)
                     /locus_tag="VEM33_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYS51044.1"
                     /translation="MVKRVLFVVLLGLLGGAACSDQRNHSQAVYMLVDTSGTYAAEVK
                     KAQVIVNYLLGTLQPGDSLAVARVRSRSFTEKDIIAKVTFDDRPSQANVQKRAFREQF
                     DKFVRTVGGSAYTDITGGLIQGAMFLNETGAGKKTILIFSDMQEELDRETIRSFPIKM
                     NGIRVVAVNVVKLKTDNIDPRKYMGRLEAWQKRVEAAGATEWRVINDIERLDAVLGRG
                     "
     gene            complement(3951..>4708)
                     /locus_tag="VEM33_04150"
     CDS             complement(3951..>4708)
                     /locus_tag="VEM33_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51045.1"
                     /translation="TDKAEHALAVSAYTQFAIATLVLLIAAGGAFVNFWLIARPMSEM
                     VGGNVYVGPMRVSEIAALVIILVEATMGLFLMEALRITHLFPVIANLQDRMRRRILWI
                     ALVLLVILAGIEAALALMRDMLIADDQALRQSLAAAPQAGRADTWLRDIPTGAQMVLG
                     FMLPFALAFVAIPLESFMHSARTAGGAALVMLVRVLAFFLRVLGNLVRQLFKVFTTLY
                     DVTIVIPLLIERLVRAARSGGEAEESRPTRRAA"
BASE COUNT          866 a         1543 c         1527 g          772 t
ORIGIN      
        1 cgagccgaag ttcgtcgagg acaagccctc gcgggtgacc tacgagatcg agccgatggg
       61 gctggtgtgc aagctcaccg tcacgcacga ccagtttccc aggggcggcc ctaccttcga
      121 agccgtgaag gagggttggc ccgagattct gagcagcctc aagtcccttc tcgagaccgg
      181 agaggcgctg gccattccgc gaaacgcgta acgaagaccg cagcttccct tcccctccgc
      241 ggcgatatac tgggcacctc gggatttcga ttcgaagggt cgtcatggaa gcggtgaagg
      301 atgcgagcca tctctacgag gtcgagcggc gggcgacgca tgccgagcgc cccggcttcc
      361 ggatcaacga gattcagatc tcgccgaagc agagagtgcc ctggcactac cacaacaacg
      421 tccaggacgc tttctacgtc ctacagggaa agatccggat ctttctccgc gatcccaagg
      481 aggaagtccg cctcggaccc ggcgagacgt tttcggtgag gccgaaacgc ccgcacctcg
      541 tgatcaacgg cggcgacgcc tccgcgacgt tcctggtgct gcagggcatc ggcgaatacg
      601 attttgtccc gctgacctaa aggacctccg cgatgctttc gacatcccgc tagcaggcgc
      661 ccgtcatgga caccatcgat cgcagcttgt ccgaagagca gcgcatgatg cgcgcgagct
      721 gccgcgcctt cgtcaacgac ttcgtcacgc ccttcatccg ccagaactgg cagcgcgaat
      781 ggctgatgga ccccgacgcg cgcctgccgc gcgccattct cgagcaggcg gacaggatcg
      841 gcatccgcac gctcggcgtc ccggaggagt tcggcggcgt gccgctcgat cccgcgcacg
      901 aagtgcagac tttcgcgctc atctccgagg agatcgcgcg cggcgactcg gggctttccg
      961 acaagctcgt gcagatctgg aaggtgtcgg tgctgctccg caacgtcgcg ccgcgccacc
     1021 tgcagcagct gtggttcccg cgcatcgtga aagacccggg cttcctgctc gctcactgcc
     1081 tcaccgagcc gcgcggcgcc tccgaccgct ggcttccgta caacgcgccg gaggcggcga
     1141 tgcagacgcg cgcggtgttg aaaggcgacg agtgggtgct caacgggcgc aagcagttca
     1201 tcagcaacgg ctacgacgcg agcctctacg tcgtttacgc caacaccagg ccgggcgcag
     1261 gcatgctgca gggcacctcc agcttcctcg tgccccgcgg cacgcccggc ctcaccatcg
     1321 cgcgctgcaa cgagacgctc ggcgggcgct tcatgaacaa cggcgagctc gttttcgagg
     1381 acatgcgcct gcccagggac cacctgctcg tcgaaaacga cgcgctcgcc aagaccgggg
     1441 tctacttcaa gccgggcaag atcatccagg cgtcgaaaaa tctcggtgtc ggcgtggctg
     1501 cattcgagcg caccgccgag tacgtgcaga actacgtgca gggcgggcgc atcctgatca
     1561 agcaccaggc ggtcgcgctg cgcctggccg agatggcgac gaagctgcgc gcggtgcgcg
     1621 ccctccttca cgaggccgcg cgtgcggtgg acgagcgagc gcccgacgcc gaggagctgt
     1681 gcaacatggt gaagctgttc gcttccgagg aagtattcaa ggtggcgcag cacgcggtcg
     1741 agctgcacgg cggcaacggc atcatgctcg acttcgggat cgagaagctt ttccgcgacg
     1801 cggcggtgtt cctgcacatg gacgcgacgg tggacatctc gaagttcaag atcgtcaagc
     1861 tgatgtttcc gcacacggcc ggcgcttacg ccggccccga agcttgagag gcttcatgca
     1921 tttgcggcaa gcatgtcttt tgactctcgg gacggcgctc ctcgcgctca cctcgcaggt
     1981 cttcgcgcag gagccctacc cgtcccgcac gatcaggatc atcgtgccct tcaccccggg
     2041 tagcgtgacc gacgtcatgg ggcgcagtgt cagcgataag ctggctgcga atctcgggca
     2101 gtcagtgatc gtggagaacc gcccgggtgc gggcgggacc atcggcatcg cccaggtggc
     2161 gaagtccgct cccgacggct acacgctcgt cgtggtttct gccggctacg ccgtcaatcc
     2221 ggtgatctac gagaatctcc cctacgactc ggcgaaggat ctcgccgggg tgatcccgct
     2281 cggaaatctt cccagcgtgc tcttcgtttc gccggcgctc ggagtgaagt cggttcagga
     2341 gctgatcgcc ctcgccaagg cgaaacccgg aacgctcaac tatccctcgg ccggaatcgg
     2401 cagcgcgtcg cacgtaaacg ccgagaagtt cctcgcggtc acggggatca aggcggtgca
     2461 cgtgccgctc aagggtgctc cggaaatgat cacggagacc atggcgggcc gcacccagtt
     2521 cggcaccgtg gggatctcgg cagcccagtc cgcgatccgc gacggccggc tgatcgcgct
     2581 cgccgtgagc ggcaagaagc gcgcgcccaa cctgcccgag gtgccgaccg tggccgaggc
     2641 cggcgtgccg ggcgccgagt tcgacttctg gatcggcgtg ctcgcgccgg ggcagacgcc
     2701 gcggcccatc atccacaagc tgcacgccga gttcgatcgc gcccttcaat cgccagacgt
     2761 gcgcgaccgc cacgccaagc tcggggcgga tcccatgccg atgagtccgg aacagttcga
     2821 cgcccagatg cgcgaggatc tcgcgacgct gggcgccctg cttcgcgccg ccggcgtgaa
     2881 ggcgaattga gtgggtcaga ggggtcggag tagtattgga cggcgtctct ttccctcaga
     2941 ccataggagg ctccatggca gcgaaacgtg aagaatacat cgcgaagctg aagacccagc
     3001 tcgatcaatg gaacgccgag atggcgaaat gggaaagcca agccgcacag gcgcaggcga
     3061 gcatgcgcgc ggagtacgag aaacagctcg ccgcgatccg ccagcatcag gaccaggcca
     3121 gggaacagtt acgcaaggtg caggcggcct cgggcgacgc ctggatggag ctctctcgcg
     3181 gcgcggacga agcctgggcg gcgatgcgca agaccttcga gaaggccagc gcgcaattca
     3241 agaagtagtt cgcggccggg aagtcccggt cgggcaggcc ggctagcccc ttccgaggac
     3301 cgcgtcgagg cgctcgatgt cgttgatcac gcgccattcg gtagctcccg ccgcctcgac
     3361 ccgcttctgc caggcttcga gccgccccat gtacttccgc ggatcgatgt tgtcggtctt
     3421 gagcttgacg acgttgaccg cgaccacgcg gatgccgttc atcttgatcg ggaaactgcg
     3481 gatcgtctcc ctgtcgagct cctcctgcat gtcggagaag atcaggatgg tcttctttcc
     3541 cgccccggtc tcgttcagga acatcgcgcc ctggatcagg ccgccggtga tgtcggtgta
     3601 ggcgctgccg ccgaccgtcc tgacgaactt gtcgaactgc tcgcggaagg cgcgcttctg
     3661 gacgttggcc tgcgacgggc gatcgtcgaa agtcaccttg gcgatgatgt ccttctcggt
     3721 gaagctgcgg cttcgcacgc gagccaccgc aagggagtcc ccgggctgca gcgtaccgag
     3781 caggtagttg acgatgactt gcgccttctt gacttcggct gcgtacgtcc ccgaggtgtc
     3841 caccagcatg tacaccgcct gggagtggtt gcgctggtcg ctgcaggcgg ccccgcccag
     3901 cagcccgagg agcactacga agagcactcg cttgaccatg gttcctcccc tcacgctgcg
     3961 cgccgagtcg ggcgcgattc ctcggcctcg ccgccggaac gcgcggccct cacgaggcgc
     4021 tcgatgagca gcggaatcac gatggtgacg tcgtatagcg tggtgaacac cttgaacaac
     4081 tggcgcacga ggtttccgag gaccctgagg aagaacgcca gcacgcgtac cagcatcacc
     4141 agcgctgctc cccccgccgt gcgcgccgag tgcatgaagg actcgagcgg gatcgcaacg
     4201 aacgcgagcg cgaagggcag catgaatccg aggaccatct gggcgccagt cgggatatcc
     4261 ctgagccaag tgtcggcgcg ccccgcctgc ggcgcggccg caagagactg gcgcaaggct
     4321 tgatcgtcgg cgatcagcat gtcgcgcatc agcgcgagag cggcttcgat gcccgccagg
     4381 atcaccagca gaaccagggc aatccacaat atgcgccggc gcatgcggtc ttggaggttg
     4441 gcaatgaccg ggaaaaggtg cgtgatacgc agcgcctcca taaggaacag ccccatcgtc
     4501 gcctcgacga ggataatgac gagcgctgcg atttccgaga cgcgcatcgg accgacatag
     4561 acattccctc cgaccatctc ggacatcggc cgcgcgatca gccagaagtt gacgaaggct
     4621 ccgcccgcgg caatcaggag caccagggtc gcgatggcga actgagtgta ggcggagaca
     4681 gccagcgcat gctcggcttt gtcggttt
//
LOCUS       DASPZD010000213         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_90797, whole genome shotgun sequence.
ACCESSION   DASPZD010000213 DASPZD010000000
VERSION     DASPZD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90797"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VEM33_04155"
     CDS             <1..569
                     /locus_tag="VEM33_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYS51046.1"
                     /translation="FFVGYWWTLLAVPAILFVGVTLAARANPLVRYQLDRLKLALPVA
                     GEILRKIILSRFASIFALMYSSGITILDAIRNSEEITGNMVIQEGLRLAGQQIGEGKN
                     VTAAFQEVGLFPPLVVRMLRVGEATGALDKALLNVSYFYNREVRESISKIQVIIEPAL
                     TVVLGVILGWVMLSVLGPIYDVISKIKI"
     gene            577..2148
                     /locus_tag="VEM33_04160"
     CDS             577..2148
                     /locus_tag="VEM33_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51047.1"
                     /translation="MSKRLCYFTSQRVITYLWTKGELHREGVFDMGEKGVAEFSRYVA
                     GAPGSLFYVLADMVEEDFFQENIPYVRGTDRRALLARKLAQRYRDASLTLPLSLGSET
                     HAGRREERVLYMSFTNTQLFQPWLEALRSNDACVAGVFSVALVAAQTGKRLGFKSGRY
                     LMVSRQQGGLRQSYIENGRIRFSRLGRTDFSDPRVIAQDCAAESLRIQQYLVNSRILP
                     REAPALDVLVLAPSEHKALYDAACVNSARLQFHVHDLDKVAGSLGLKSAPADTLAEGL
                     FLHVLAAYPPREQYADERLRRFYHLWRARVALLATGAAVFASCLLLSAVRLIDIYQVN
                     EQAQNDRVQEARASEEYARLQKRFPKTPISSETLKTVVKNYRALLRQSGSPGRMFAEI
                     SEAVTALPQIEIDRIDWEFGTGAKSAAGREASKAPPASPTGQAQAASTEFQSQTAEIA
                     GKLVVQQASDFRAVTALVNQFTEALRRRPGTEVTRTQLPFDINAEKSLAGDIGAARSE
                     EVPQFSVIVVKRRGT"
     gene            2145..>2470
                     /locus_tag="VEM33_04165"
     CDS             2145..>2470
                     /locus_tag="VEM33_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51048.1"
                     /translation="MNITRDDLKRLRVPLAVAIVLLVLGAACLIAGGYHLDEARAARD
                     ATRLSRVAAQERVLRVAEEEREIRDDLVYYEQMRQRGIVGEQSRLDWIESIARIKNDR
                     KLFEIR"
BASE COUNT          444 a          789 c          790 g          447 t
ORIGIN      
        1 cgtttttcgt aggctactgg tggactctcc tggctgtccc cgcgatcctg ttcgtcggcg
       61 tcacgctggc ggcccgggcc aaccccttgg tgcgttacca gctcgatcgc ctcaagctcg
      121 ctctcccggt ggcgggcgag atcctgcgca agatcatcct gtcgcgcttc gccagcatct
      181 tcgctctcat gtactcctcg gggatcacca tcctcgatgc gatccgcaac tcggaggaaa
      241 tcaccggcaa catggtcatc caggagggcc tgagactcgc gggacagcag atcggcgagg
      301 gcaagaacgt caccgctgcg ttccaggagg tggggctttt cccgccgctc gtcgtgagaa
      361 tgctgagggt gggcgaggcg accggcgcgc tcgacaaggc gctgctcaac gtgtcctact
      421 tctacaaccg ggaagtgcgc gaatcgatca gtaagattca agtgatcatc gagccggcgc
      481 tgacggtggt cctcggcgtc attctcggct gggtgatgct ctccgttctc gggccgattt
      541 acgacgtcat cagcaagatc aagatctagg gccgtggtga gcaaacgtct ctgttacttc
      601 acttcccaga gagtcatcac ctatctatgg acgaagggtg agctgcacag ggaaggtgtt
      661 ttcgacatgg gcgagaaagg cgtcgccgaa ttctcccgat acgtggccgg cgccccggga
      721 agccttttct acgtgctcgc cgacatggtc gaggaagact tcttccagga gaacattcct
      781 tacgtgcgcg gcacggaccg gcgcgcgctg cttgcgcgca agctcgccca gcgttaccgc
      841 gacgcgtcgc tcaccctccc gctttcgctc gggtcggaga cccacgcggg ccggcgcgag
      901 gaacgcgttc tctacatgtc gttcaccaat acgcagctgt tccagccctg gctcgaggcc
      961 ttgcgctcga acgatgcctg cgtggccggc gtattttctg tggcgctcgt cgcggctcag
     1021 acggggaagc gtctcggttt caagagcggg cggtacctga tggtgagccg gcagcagggc
     1081 ggactgcgcc agagctacat cgaaaacgga cgaatccgct tctcgcgcct cggccgcacg
     1141 gacttctccg atccccgtgt gatcgcccaa gattgcgccg ccgaatcgct gcgaatccag
     1201 cagtatctcg tcaacagccg tatcctgccg cgcgaggcgc ctgcgctcga cgtgctggtg
     1261 cttgcgccga gcgagcacaa ggcgctgtac gacgcagcct gcgtcaattc cgcccggctg
     1321 cagttccatg ttcacgacct cgacaaggtc gccggcagct tgggcctcaa atcggcgccc
     1381 gccgatacgc tcgccgaggg actgtttctc cacgtgctcg ccgcgtaccc gccccgcgaa
     1441 cagtatgccg acgagcgcct gcggcgcttc tatcatttgt ggcgcgcgcg cgtggccctg
     1501 cttgccaccg gggcagcggt gttcgcctct tgcctgctcc tttcggcggt gagacttatc
     1561 gatatctatc aggtaaacga gcaggctcag aatgaccgcg tgcaggaagc ccgtgcgtcc
     1621 gaggaatatg ctcggctgca gaagcgtttc cccaagacgc cgatctccag cgaaaccctg
     1681 aagacagtcg tcaagaacta ccgggcactc ctgcggcaga gcggttcgcc cggcaggatg
     1741 ttcgccgaga tcagcgaagc ggtaacggcc ctgccccaga tcgaaatcga caggatcgac
     1801 tgggagttcg gcaccggagc gaagtcggcg gccggccgcg aagcgtccaa agctcctcct
     1861 gcttcgccta cagggcaggc acaggccgcg agcaccgagt ttcagagtca aaccgccgag
     1921 atcgcgggca agctcgtcgt tcagcaggct tccgacttcc gcgcagtgac ggccctggtg
     1981 aaccaattta ccgaggcgct gcgcaggcgg cccggcaccg aagtgacgcg cacccagctg
     2041 cccttcgaca tcaacgcgga aaagagcctc gccggcgata tcggggcggc gaggagcgag
     2101 gaagtgccgc agttctcggt gatcgtggtc aagcggcgcg gcacatgaac atcacccgcg
     2161 acgacctcaa gcgcctgcgc gtgcccctcg cggtggcgat cgtgctgctt gtgctaggcg
     2221 cggcttgcct gatcgccggc ggatatcacc tggacgaggc gagagcggcg cgggacgcga
     2281 ctcggttgag ccgcgtggcg gcgcaggagc gggtcttgag agtcgcggag gaggagcgcg
     2341 agatccgcga tgacctcgtc tactacgagc agatgcgcca gcgcggcatc gtgggcgaac
     2401 agagccggct cgactggatc gaatcgatcg cgaggatcaa gaacgatcgc aagctcttcg
     2461 agatcaggta
//
LOCUS       DASPZD010000214         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_51405, whole genome shotgun sequence.
ACCESSION   DASPZD010000214 DASPZD010000000
VERSION     DASPZD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51405"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VEM33_04170"
     CDS             <1..490
                     /locus_tag="VEM33_04170"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HYS51049.1"
                     /translation="DYRASERAFAQLQQVAGRAGSANLPGEVLIQTRYPEHPLYQSLV
                     RHDYAGFAKSVLAERREAGFPPFVFEAALRAESTDELRAQRFLRTAIDQSPHRSASIA
                     VFDPVPMSLARLAGAERAQVLVQSRSRPRLQAFLSEWSDALYRMPSHGVRWHLDVDPI
                     EF"
     gene            701..2365
                     /gene="argS"
                     /locus_tag="VEM33_04175"
     CDS             701..2365
                     /gene="argS"
                     /locus_tag="VEM33_04175"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYS51050.1"
                     /translation="MSAEVKSHLTELLTRALAKVAPQQPGSLISLERPKQVQHGDFSC
                     PAAMQLARSLKRNPRELALELVAALPPSEWLERAEVAGNGFINLRVRPVAKQHIVQTI
                     LTEGDRYGRADRAGGRPVMVEFVSANPTGPLHVGHGRQAALGDAIGALLESQGWRVTR
                     EFYYNDAGAQIRNLGLSVQARIREKLGVPAEFPADGYHGDYVREVAERFLEAGNADGE
                     DLEAVTRFAVAELRREQDLDLQAFGVRFDSYYLESSLYGDGRLEKTVQALVDSGKTFE
                     RDGALWLRTTEYGDDKDRVMRKSDGTYTYFVPDVAYHLAKWERGFNRAINIQGSDHHG
                     TVARVRAGLQAASSKWERAVPRGYPDYVLHRMVTVMKGGEEMKISKRAGSYVTLRELI
                     DEVGRDAVRFFLLSRKADADFVFDIDLAKSQSEENPVYYVQYAHARIHSVLEQWRAQF
                     GGGNFDSFRNVDLAPLGGARELGLLQRLGEYAEILENAEAELAPHQVVFYLRELAGEF
                     HSYYNAERILVAEAPLRSARVALCLAVRQVLRNGLSLLGVSQPEKM"
     gene            2378..2962
                     /locus_tag="VEM33_04180"
     CDS             2378..2962
                     /locus_tag="VEM33_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYS51051.1"
                     /translation="MSFNYKQVPRSRPDFSGNFVIGLFFGFLLGLGVAAGIAIYFFKT
                     PIPFADRQRPPDKAPPVDQKLAEPPKSAKAEDKPRFDFYRILPGKEEPVTERQMREAA
                     KQAAKPGAPKDSYFLQAGSFQNPADADNLKARLALMGMEANVEPANLAEKGVWYRVRL
                     GPYTRVDEINRVRQQLTQNGVDSSLVKITEVPKN"
     gene            2984..3631
                     /locus_tag="VEM33_04185"
     CDS             2984..3631
                     /locus_tag="VEM33_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HYS51052.1"
                     /translation="MVFRLLSVLSGLFFALAAHAQSSPVEGVDYIELKPPQAVESSGR
                     IEVLEFFWYRCPHCYALEPDLESWAKRLPRDVQFKRLPGILNDDWAVDARIFYTLEAI
                     GELERLHRPFFDTIHQQGGVRLRGDAFAKWAADWLAKQNVDMAKYDAAFRSFTVESKL
                     RRAAQMGRSYRLDGVPALAVQGRYLVVASTSRKAMLATADFLIGEARKQLAKAKP"
     gene            3665..4438
                     /locus_tag="VEM33_04190"
     CDS             3665..4438
                     /locus_tag="VEM33_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYS51053.1"
                     /translation="MSPDPKVFITGASSGIGAALAAHYAGHGAVLGLCARRLAPTQAL
                     LSSLSCKGSCYQVDVTDATSLQAAAADFTERFGVPDIVIANAGISVGNSTENAEDLPA
                     FRKIMDTNVFGMAVTFQPFVAPMRARRSGTLVGIASVAGFRGLPGGSAYSASKSAAIT
                     YLESLRVELHGSGVAVVTICPGYIRTAMTASNRYRMPFIIDADDAARRFARAIAGKRR
                     FAVIPWQMALVSFALRRLPRWLFDRLFARAPRKARGLPL"
     gene            4435..5265
                     /locus_tag="VEM33_04195"
     CDS             4435..5265
                     /locus_tag="VEM33_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HYS51054.1"
                     /translation="MSGPEARLVDAVGTRHAPARREARIVSLVPSITELVCDLGLAAQ
                     LVGRTGFCVHPREVVRRIPKVGGTKDVDLDKLRALRATHVILNIDENREEDARAIAGF
                     VPELIVTHPLAPLDNLALYRLIGGVFGREDQAEALCGEFEAAYAALQSAARNFPPDRV
                     LYLIWKNPWMTVSRDTYVSRMLALVKWETVPADCADRYPKIELNSEVLDGARVVLLSS
                     EPYLFREAHVSELRAESFMRERNIALIDGEMTAWYGSRAIEGLRYLRRFRERLDRTAG
                     "
     gene            complement(5273..>5617)
                     /locus_tag="VEM33_04200"
     CDS             complement(5273..>5617)
                     /locus_tag="VEM33_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HYS51055.1"
                     /translation="STLEDHVAPWKSTYKGARLLSGPTRFVLGGSGHIAGIVNPPSAK
                     KYGYWTGSELPESAEEWQAGATRSEGSWWIDWQGWIAGLNGPDRAPARVPGAGALQAL
                     EDAPGSYVSVPA"
BASE COUNT          977 a         1831 c         1805 g         1004 t
ORIGIN      
        1 cgactatcgg gcgagcgagc gggcctttgc ccagttgcag caagtagcag gacgcgccgg
       61 aagcgcgaat ctgccgggcg aggtgctgat ccagacgcgc tatccggagc atcctctgta
      121 tcagtcgctc gtgcgtcatg actacgcagg atttgcaaag agcgtgctgg cggaacggcg
      181 ggaggcgggg tttccgccct tcgttttcga agctgcgctg cgcgcggaaa gcaccgatga
      241 gcttcgcgcg cagcgcttcc tccgaacggc catcgaccag tcaccgcacc ggagcgcttc
      301 tatcgccgtg ttcgatccgg taccgatgtc gctcgcgcgg ctcgcgggtg cggagcgcgc
      361 acaagtgctc gtgcaatcgc gctcgcggcc gcgcctgcaa gcgtttctct cggaatggag
      421 cgatgccctc taccgcatgc cctcgcacgg agtgcgctgg catctcgacg tagacccgat
      481 agagttttga ccgcctgcgg aaatcaggca ggtctcttcg cgggtttttt cgccttacgc
      541 ggtcgagtct tgccgtgcga ccctttgaag actttgccgc ggaatgtgcg tttatcgcct
      601 ttgcccaagt tcgaactcca tgtcggaaga ttgaggcaga cggtattcta cctttgcacg
      661 accggcgatg atcaatgccc gcgtataata cggcccctcg atgtccgccg aggtcaaatc
      721 ccacctcacc gaattgctca cccgtgcgct cgccaaagtc gccccgcagc aaccgggttc
      781 gctgatttca ctggaacggc ccaagcaggt gcagcacggc gatttttcct gcccggcggc
      841 catgcagctt gcgcgttctc tcaagcgcaa tccgcgggag ctggcccttg aactggtcgc
      901 ggcgctgcct ccatccgaat ggctcgagcg cgctgaagtc gccggcaacg ggttcatcaa
      961 cctgcgcgtt cgccctgtgg ccaagcagca catcgtccag accattctca ccgaaggcga
     1021 ccgctacggc cgcgccgacc gtgccggcgg ccggccggtg atggtggagt tcgtatcggc
     1081 caatcccacc ggcccgctgc acgtcgggca cggaaggcaa gcggccctcg gcgatgcgat
     1141 cggcgccctg ctcgaatccc agggctggcg cgtaacgcgc gagttctatt acaacgacgc
     1201 gggcgcgcag atccgcaatc tgggactctc ggtgcaggcg cggattcgag aaaaactcgg
     1261 cgtaccggcc gaatttcccg ccgacggcta tcacggggac tacgtgcgcg aggtcgccga
     1321 aagatttctc gaggcgggca acgccgacgg cgaggatctg gaagccgtga cccgctttgc
     1381 ggtggccgag cttcggcgcg agcaggatct cgatctgcag gctttcggcg tgcgcttcga
     1441 cagctattat ctggaatcct cgctatacgg ggacggccgc cttgagaaaa cggttcaggc
     1501 gctcgtcgac tcgggcaaga ccttcgagcg ggacggcgcg ctctggctca ggaccaccga
     1561 gtacggcgac gacaaggacc gcgtcatgcg caaatcggac ggcacctata cgtatttcgt
     1621 gcccgacgtc gcctaccatc tggcgaagtg ggagcgcggt ttcaaccgtg ccatcaacat
     1681 ccaggggtcc gatcaccacg gcacggtcgc gcgggtgcgc gccgggctgc aagccgcgtc
     1741 ttcgaaatgg gagcgcgcgg ttccgcgggg ctatcccgac tatgtgctcc acaggatggt
     1801 cacggtaatg aagggcggcg aggaaatgaa gatctccaag cgcgccggat cctacgttac
     1861 gctgcgcgag ctgatcgacg aagtcgggag ggacgccgtg cggttcttcc tgctttcgcg
     1921 caaggccgat gcggatttcg tattcgacat cgatctcgcc aagtcgcagt ccgaagagaa
     1981 tcccgtctac tacgtgcagt acgcgcacgc ccggatccac tcggtgctgg aacaatggag
     2041 agcgcagttc ggcggtggca atttcgactc gttcaggaat gtcgatctcg cgccgctcgg
     2101 cggggcacgc gagctcgggc ttctgcaacg actgggcgag tacgccgaaa tcctcgagaa
     2161 cgccgaggcg gagctcgctc cgcaccaggt cgtgttttat cttcgcgagc tggcgggtga
     2221 atttcacagt tactataatg ccgaacgcat cctggtcgcc gaggcgccgt tgcgatcggc
     2281 gcgcgtggct ctgtgccttg cagtgcgcca agtgcttcgc aacgggcttt cgctgctggg
     2341 cgtcagccag cccgagaaaa tgtgacgcct tacctctatg agcttcaact ataagcaagt
     2401 gccccgatcc agaccggact tttccggtaa ttttgtgatc ggcctttttt tcggttttct
     2461 gctcggcctg ggcgtagcgg ccggaatcgc gatctatttc ttcaagacgc ccattccgtt
     2521 cgccgacagg cagcggccgc cggacaaagc tccgccggtc gatcagaagc tggccgagcc
     2581 ccccaagtcg gccaaggcgg aggacaagcc tcgcttcgat ttctaccgga tcctccccgg
     2641 aaaggaggag ccggtcacgg aacgccagat gcgcgaggcg gccaagcagg ccgccaagcc
     2701 cggcgcaccc aaggacagct atttccttca agccggctcg ttccagaacc cggccgatgc
     2761 ggacaacctc aaggcaaggc tcgccttgat gggcatggaa gcgaacgtgg agccggccaa
     2821 tctcgcggaa aaaggcgtgt ggtatcgagt gcggctgggg ccttacaccc gcgtggacga
     2881 gatcaaccgt gtccggcaac agctgacgca gaacggagtg gactcttcgc tggtcaaaat
     2941 caccgaagtt cccaagaatt gaatcgcccc aaggagaagc cccatggttt ttcgtctgct
     3001 gagcgtccta tccggcctgt ttttcgctct cgccgcgcac gctcagagtt cgcccgtcga
     3061 aggcgtcgat tacatcgaat tgaagccgcc ccaggccgtc gagtcttcgg gcaggatcga
     3121 agtgctcgag tttttctggt accgctgccc gcactgctac gcgctggagc ccgacctcga
     3181 atcctgggcg aagcgattgc cgcgcgacgt gcaattcaag cgcctgccgg gaattctgaa
     3241 cgacgactgg gcggtcgatg cacggatttt ctacacgctg gaggccatcg gagagctcga
     3301 gcggctgcat cgacctttct tcgacaccat ccaccagcag ggcggcgtgc ggctgcgcgg
     3361 cgatgccttc gccaaatggg cggcggactg gctcgccaag cagaacgtgg acatggccaa
     3421 atacgacgcc gctttccgct cgtttacggt cgaaagcaag ctgcgccgcg cggcccagat
     3481 gggccgctcg taccgactcg acggagtacc ggctctggcc gtgcagggcc gctacctcgt
     3541 cgtcgcgagc acgtctcgca aggcgatgct cgccaccgcc gactttctca tcggagaagc
     3601 gcgaaagcag ctcgcaaagg cgaagcccta gcgcggcggg gccagcgacg cgttctgccg
     3661 tccggtgtca cccgacccca aggtcttcat cactggcgca tcgagcggga tcggcgccgc
     3721 gctcgcggcg cactacgcgg gccacggggc ggtgctcggc ctctgcgcaa gacggctggc
     3781 cccgacgcag gcgctcctct cgagcctctc ctgcaagggg tcctgctatc aagtggacgt
     3841 gaccgacgcc accagcctgc aggctgcggc ggccgatttc acggagcggt tcggtgtgcc
     3901 cgacatcgtg atcgcgaatg ccggcatcag cgtaggcaac tccaccgaga atgcagagga
     3961 ccttcccgcg ttccgcaaga tcatggacac aaacgtgttc ggaatggccg tgacgtttca
     4021 gccgtttgtt gcgccgatgc gagcgcgccg cagcggcacg ctggttggca tcgcgagcgt
     4081 cgccggcttt cgcgggctgc ccggcgggag cgcttacagc gcttccaaat cggcggcaat
     4141 cacgtatctg gagagcctgc gcgtggagct ccacggctcg ggcgtggccg tggtcacgat
     4201 ctgtcccggc tatatccgca ctgcgatgac cgcgtcgaat cgctaccgga tgccgttcat
     4261 catcgacgcg gacgatgcgg cgcggcgctt tgcccgggcc atcgccggaa agcgccgctt
     4321 cgcggtcatc ccctggcaga tggcgctcgt ttcctttgcg cttcgccgcc tgccgcgctg
     4381 gctgttcgac cggctgttcg cgcgcgcgcc gagaaaggcg cgcggcctcc ccttgtgagc
     4441 gggccggagg cgagactcgt cgacgccgtg gggacgcggc acgcgccggc gcggcgggag
     4501 gcgcgcatcg tgagcctggt gcccagcatt acggagctcg tctgcgatct cggactcgcg
     4561 gcacagctcg tcggccgaac cggcttctgc gtgcatcccc gcgaggtcgt tcgccggatc
     4621 cccaaagtcg gcggcaccaa ggatgtcgat ctcgacaagc tgcgcgcgct gcgggccacc
     4681 cacgtcatcc tcaatatcga cgagaacagg gaggaggacg ctcgcgcgat cgccgggttc
     4741 gttcccgagc tgatcgtcac gcacccgctc gcgccgctcg acaacctggc tctctaccgg
     4801 ctgatcggcg gagtattcgg gcgagaggac caagccgaag cgctgtgcgg cgaattcgaa
     4861 gcagcgtacg cggcgctgca gtcggcggca cgcaacttcc cgccggaccg cgtgctctac
     4921 ctgatatgga aaaatccgtg gatgacggtg tcgcgcgata cctacgtttc gcggatgctc
     4981 gcgctcgtaa aatgggaaac cgtcccggcg gactgcgccg atcgctaccc gaagatcgag
     5041 ctcaacagcg aggtactcga cggcgcccgg gtcgtgctgc tgtcgagcga accgtacctc
     5101 ttccgcgagg cgcatgtctc cgagctgcgc gccgaatcgt tcatgcggga gaggaacatc
     5161 gctctcatcg acggagagat gaccgcgtgg tatggcagcc gcgccatcga gggtctgcga
     5221 tatctccggc gattccgcga gaggctcgac cgcaccgcag gataattccg gctcaggcgg
     5281 gaacgctcac ataagacccc ggagcgtcct cgagggcttg cagtgccccc gcgcccggaa
     5341 cgcgggccgg cgcccggtcg ggcccgttca gaccggcgat ccagccctgc cagtcgatcc
     5401 accacgaacc ttcgctccgg gtcgcccctg cctgccactc ctcggccgat tcggggagct
     5461 cggatccggt ccagtagccg tattttttcg ccgacggcgg attcacgatg ccggcgatgt
     5521 gccccgagcc cccgagcacg aaccgcgtgg gacctgagag gaggcgggcg cccttgtacg
     5581 tcgatttcca cggcgcgacg tggtcctcga gggtgga
//
LOCUS       DASPZD010000215         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1250539, whole genome shotgun sequence.
ACCESSION   DASPZD010000215 DASPZD010000000
VERSION     DASPZD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250539"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VEM33_04205"
     CDS             complement(<1..461)
                     /locus_tag="VEM33_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HYS51056.1"
                     /translation="MELPANHFKRALKAGKRQIGLWCSLSHHYATELVAGSGFDWILL
                     DTEHSPNDLESVLRQLQAAAPYPTHPVVRVPWNDMVTIKRFLDIGAQSILIPYVQNAE
                     EAKSAVAYTRYPPAGVRGVAGGTRATRFARVKDYAKRAHEELCVLVQVETQ"
     gene            complement(461..1135)
                     /locus_tag="VEM33_04210"
     CDS             complement(461..1135)
                     /locus_tag="VEM33_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HYS51057.1"
                     /translation="MTAIGFRILPIPKRPDKKLVAELARMVTPHLSDSMERLYAGGPQ
                     LRPMHNGAKLCGPAFTVRTAPGDNLLVHKAIDTAEPGDVIVVDGGGFNDHALIGELMS
                     ARAEQRGVGGMVIWGAIRDSAELKEGSFPVFASGVTHRGPYKNGPGEINVPITIGGIP
                     VSPGDIIVGDADGLVAVPQEHAQEILASAKAILAKEENSMKQIKAGTVDRSWVDKALK
                     EKGYKV"
     gene            complement(1147..1932)
                     /locus_tag="VEM33_04215"
     CDS             complement(1147..1932)
                     /locus_tag="VEM33_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioate hydratase"
                     /protein_id="HYS51058.1"
                     /translation="MPLSEAERKKCVETLLNAEKEKKQAVQLSVTYPHITIEDSYAIS
                     TAVAQHRIKAGARLIGHKVGLTSKAMQASSQINEPDYGHLLDYMMIPDGAKVPHANYC
                     LPRVEIELAFILGKPLKGPGIGLTDVLHATEYVIPAMEVVDARIVNPRKIFDTVADNG
                     AAAGIVVGGRPIGPMEMDLRWAGGIMYRNSEIEETGLAAGVLGHPALGVAWLANRLGK
                     SGVTLEAGHLILAGSFTRVVFANKGDTLHGDFGPLGGIAVQFV"
BASE COUNT          307 a          667 c          680 g          392 t
ORIGIN      
        1 tgctgggtct ccacctgcac cagaacgcag agctcctcgt gggcgcgctt cgcgtagtcc
       61 ttcacgcgcg caaagcgcgt cgcccgcgtg ccgccggcga cgccgcgcac gcccgcgggc
      121 ggatagcgcg tgtacgcgac cgcgcttttc gcttcttcgg cgttctgcac gtagggaatc
      181 aggatcgatt gcgcgccgat gtccagaaaa cgcttgatcg tcaccatgtc gttccagggc
      241 acgcggacca cggggtgcgt cggatagggt gcggccgcct gcaattgccg cagcaccgac
      301 tcgagatcgt tcggcgagtg ctcggtgtcg agcaggatcc agtcgaagcc cgagcctgcg
      361 acgagctcgg tcgcgtagtg gtgggaaagg ctgcaccaca ggccgatctg ccgcttgccg
      421 gccttcagcg cgcgcttgaa gtgattcgct gggagttcca tcagactttg tagcccttct
      481 ccttgagcgc cttgtcgacc cagctgcggt cgaccgtgcc cgccttgatc tgcttcatcg
      541 agttttcttc cttcgcgagg atcgctttcg ccgaagcgag aatttcctgc gcgtgctcct
      601 gcggaacggc gaccaggccg tccgcgtcgc cgacgatgat gtcgccggga ctgaccggga
      661 tgccgccgat cgtgatgggc acgttgatct cgcccggccc gttcttgtag gggccgcggt
      721 gcgtgacgcc gctcgcaaag acggggaagc tcccttcctt gagctccgcc gagtcgcgga
      781 tcgcgcccca gatcaccatt ccgcccacgc cgcgctgctc cgcgcgcgcc gacatcagct
      841 cgccgatcag ggcgtggtcg ttgaatccgc cgccgtccac cacgatcacg tcgcccggct
      901 cggccgtatc gatcgccttg tggacgagca ggttgtcgcc gggcgcggtg cgcacggtaa
      961 acgccgggcc gcagagcttc gcgccgttgt gcatggggcg aagctgcggt ccgcccgcgt
     1021 acagccgctc catgctgtcg gagagatgag gagtcaccat cctggcgagc tccgcgacga
     1081 gcttcttgtc ggggcgcttc gggatgggga ggatgcgaaa gccgattgca gtcatgagcg
     1141 tggcccctag acgaactgca ccgcgatccc gccgagcggc ccgaagtccc cgtgcagggt
     1201 gtcgcccttg ttcgcgaaca cgacgcgcgt gaacgagccg gcgagaatca ggtgcccggc
     1261 ttcgagcgtc acgccgcttt ttcccagccg gttggcgagc caggcgacgc cgagcgccgg
     1321 gtggccgagc acgcccgcgg cgaggcccgt ctcctcgatc tccgagttgc ggtacatgat
     1381 gccgcccgcc cagcgcaggt ccatttccat cgggccgatc gggcggcccc cgacgacgat
     1441 gccggccgcc gcgccgttgt cggcgacggt gtcgaaaatc ttgcgcgggt tgacgatacg
     1501 cgcatcgacg acctccatcg cgggtatcac gtactcggtc gcgtgcagca cgtcggtgag
     1561 cccgattccc ggtcctttca gcggcttgcc gagaatgaaa gcgagctcga tctcgacacg
     1621 tgggaggcag tagttcgcgt gcggcacctt ggcgccgtca ggaatcatca tatagtcgag
     1681 caagtgtccg tagtcgggct cgttgatctg cgaggaggcc tgcatggcct tcgaggtgag
     1741 cccgaccttg tgtccgatga gcctcgcgcc cgccttgatc ctgtgctgcg cgaccgcggt
     1801 cgagatggcg tacgaatcct cgatggtgat gtggggatag gtgactgaga gctgcaccgc
     1861 ctgctttttc tccttctcgg cgttcagcag cgtctcgacg catttcttgc gttcggcttc
     1921 ggacagcggc atgggttccc ctcttttcgg aatgcggatt ttacactgcc ggggatcgcg
     1981 gcaatgccgc cgcggaatcg aggatgaagc taggatagcg gttcatcgcg ccctggagga
     2041 ggtcat
//
LOCUS       DASPZD010000216         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_488310, whole genome shotgun sequence.
ACCESSION   DASPZD010000216 DASPZD010000000
VERSION     DASPZD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488310"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /gene="ppsA"
                     /locus_tag="VEM33_04220"
     CDS             complement(<1..1264)
                     /gene="ppsA"
                     /locus_tag="VEM33_04220"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HYS51059.1"
                     /translation="MTRLKYVVNFDRLGMGDVERVGGKNASLGEMIVQLSKAGIRVPG
                     GFATTAAAFREFLAAGGLASRIEARLKGLDADDVEALGRCGAEIRGWIEATPFPKQLE
                     AEIGQAFRELTGGDESVSVAVRSSATAEDLPDASFAGQQETFLNIRGLANVMTALKHV
                     FASLYNDRAISYRVHRGFGHSAVALSAGVQRMARSDLGASGVMFTLDTESGFRDVVFI
                     TASWGLGELVVQGAVNPDEFYVHKPMLRGGKFPVIRRGLGSKLQKMVFAEQARTGLSV
                     KTVDVSEADRNRFCLSDEDVIELARFALRIEEHYQRPMDIEWAKDGADGKIYILQARP
                     ETVKSRKSAEGQERFLLKQKSEVLATGRAIGHKIGAGTVRVVKDASEMARIKPGDIIV
                     TDMTDPNWEPVMKRAAAIVTNRGGRTCHA"
     gene            1365..2192
                     /locus_tag="VEM33_04225"
     CDS             1365..2192
                     /locus_tag="VEM33_04225"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HYS51060.1"
                     /translation="MPDPRRTVFFVSDGTGITVEMLGHSLLTQFDGIEFDQRTIPFID
                     SVEKAQACVSRINEASASGSGRPVVFTTLVNAEIRETVRKAEAFVLDFFESFLDPLEA
                     EFGAKSTHTIGRSHSARDAKGYTHRIDAINFALAHDDGASSRDLDKADVILVGVSRSG
                     KTPTSLYLSLQFGIRAANCPLIPEDFDRMSLPTALQAYRRKLYGLSIAPERLREIRNE
                     RRPNSKYADLENCRYEVQQAEKLMSREGIRWANSTTKSIEEIATTIMQEFKLERRGY"
     gene            complement(2189..2971)
                     /locus_tag="VEM33_04230"
     CDS             complement(2189..2971)
                     /locus_tag="VEM33_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYS51061.1"
                     /translation="MKSIRSRDNPQVKALIKLAGSSRERRRTGTTILEGEHLVRAYQQ
                     SGGIAEVVLAGETALARLEIRDFFENVPAKSRLVLADELLERVSQLASSAGIAAVIRT
                     PLPGPIPQNISSCLLLENIQDPGNLGAILRTAAAAGITHILLSKDSVFAWSPKVIRAG
                     MGAHFFLSISEGADIGEFAQSFRGSVVAMGPRAEASLYDLDLKGPVAWVFGNEGAGLS
                     ERAGHLATHRVRIPMPGSAESLNVAAAVAICLFEQIRQRVAA"
     gene            complement(2968..4179)
                     /gene="senA"
                     /locus_tag="VEM33_04235"
     CDS             complement(2968..4179)
                     /gene="senA"
                     /locus_tag="VEM33_04235"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HYS51062.1"
                     /translation="MSPESLSRQLRDARRRTRLITCDLEGPQLFGPKLAIVNPVLWEL
                     GHVAWFQELWCLRLRSDESLSESVLEGADALYDSAKVAHDTRWDLPLPELSATLSYQD
                     RVLDRVLARLEREPDNEWFLYFVQLALFHEDMHAEAFHYTRQTLGYADPLDRGSPRKG
                     ALDGDVELRGGEFLLGATRNSGFHFDNEKWAHPVKLDPFRIARAPVTNSQFLEFVRDE
                     GYQRRDLWSPEGWTWKDRARGPRYWVERDREWRERRFDEEVRLDGDLPVMHVNWHEAN
                     AYCRYAGRRLPSEAEWEMAAAFSRVTAANLGGSGRVPVGEFAEGDSAAGCRQMLGNVW
                     EWTSSTFAPYPGFVRDPYAEYSEPWFGTHKVLRGGSFATPARLISNTWRNFYTPDRGD
                     VFAGFRTCALE"
     gene            4199..>4493
                     /locus_tag="VEM33_04240"
     CDS             4199..>4493
                     /locus_tag="VEM33_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04348 family glycosyltransferase"
                     /protein_id="HYS51063.1"
                     /translation="MKIALVTPARARSRSGNRHTATRWAAMLRSLGHRVQISTRWDGR
                     AADAMIALHARRSHDSIRRFRERFPALPLVVVLTGTDLYRDIRTDRDAQDSLVL"
BASE COUNT          812 a         1500 c         1398 g          783 t
ORIGIN      
        1 ccgcgtggca ggtgcggcct ccacggttgg tgacgatggc cgcggcgcgc ttcatcacag
       61 gctcccagtt ggggtcggtc atgtccgtta cgatgatgtc gccgggtttg atgcgcgcca
      121 tctccgaggc gtccttcacc acgcgcaccg tccccgcccc gatcttgtgg ccgatggcgc
      181 ggcccgtggc gaggacctcg gacttttgct tcaggaggaa gcgctcctgc ccttccgccg
      241 atttgcgcga cttcacggtt tcgggcctcg cttgcaggat gtatatcttc ccgtcggcgc
      301 cgtccttcgc ccactcgatg tccatggggc gctggtaatg ctcctcgatc ctgagcgcga
      361 agcgcgcgag ctcgatcacg tcttcgtcgc tcaggcaaaa gcggttgcgg tcggcctcgg
      421 acacgtccac cgtcttcacc gaaaggcccg ttcgggcctg ctcggcaaac accattttct
      481 gcaatttcga gccgagacca cgccggatta ccgggaactt gccgccgcgc agcatcggct
      541 tgtggacata gaactcgtcc gggttgacgg caccctgcac gaccagctca cccaggcccc
      601 aggacgcggt gatgaatacc acgtcgcgaa agccggactc ggtgtcgagc gtgaacatca
      661 ccccactcgc gcccagatcg ctgcgcgcca tgcgttgcac gccggccgaa agcgccactg
      721 ccgagtgccc gaatcccctg tgaacgcggt aggaaatggc gcggtcgttg tagagcgagg
      781 cgaacacgtg cttgagcgcg gtcatcacgt tggcgaggcc gcggatattg aggaaagttt
      841 cctgctgtcc ggcgaaagag gcgtcgggca ggtcttcggc cgtggcgctg gaacgcactg
      901 ccaccgaaac gctctcgtcg cctccggtca gctcgcggaa agcctgcccg atctccgctt
      961 cgagctgttt cggaaagggc gtggcttcga tccagccgcg gatctccgcg ccgcaccgcc
     1021 cgagcgcttc gacgtcgtcg gcgtccagtc ccttcaggcg cgcctcgatg cgcgacgcca
     1081 ggccgcctgc tgcgaggaat tcgcggaacg ccgcggcggt ggtggcaaaa ccgccgggta
     1141 cgcggattcc cgccttggag agctggacga tcatctcgcc cagcgacgcg ttcttccctc
     1201 cgacgcgctc gacgtctccc attccgagcc gatcgaaatt caccacgtat ttgagccggg
     1261 tcatggagcg gtccttcgcc gcggttgaag gatcatcttg aaaccgttat gttacaggga
     1321 gcgcattccg cgcgcagaca aaataccccg ccaggcccct tttcatgccc gatccccgtc
     1381 gaaccgtgtt cttcgtctcc gacgggacgg ggatcacggt ggagatgctc ggccacagcc
     1441 tgctcacgca gttcgacggc atcgaattcg accagaggac gattcccttc atcgactccg
     1501 tcgagaaggc ccaggcctgc gtgagccgga tcaacgaagc ctcggcttct ggcagcgggc
     1561 ggccggtggt attcaccacg ctcgtcaacg ccgagatacg cgagaccgtg cgcaaggccg
     1621 aggccttcgt gctggatttc ttcgagagct ttctggatcc gctcgaggcg gagttcggcg
     1681 cgaaatccac ccacacgatc ggccggtccc acagcgcgcg ggacgcaaag ggctacaccc
     1741 accgcatcga cgcgatcaat ttcgccctcg cccacgacga cggggcctcg tccagggacc
     1801 tcgacaaggc ggacgtgatc ctggtcgggg tgtcgcgcag cggcaagacg cccaccagcc
     1861 tctacctttc cctccagttc ggaattcgcg cggcgaattg cccgctcatt cccgaggatt
     1921 tcgaccggat gagcctgccc actgcactgc aggcctaccg ccgcaagctg tacggcctgt
     1981 ccatcgcgcc ggagcggctg cgggagatcc gcaacgagcg caggcccaac agcaagtacg
     2041 cagacctgga gaattgccgc tacgaagtgc agcaggcgga gaaactgatg tcgcgcgaag
     2101 gcatacgctg ggcgaattcg acgacgaaat cgatcgagga gatcgccacg acgatcatgc
     2161 aggagttcaa gctggagcgc aggggatact aagcggcgac tcgctggcga atctgctcga
     2221 agaggcagat cgccaccgcc gcggcgacgt tcagcgactc tgcggaaccg ggcatgggga
     2281 tgcgcacgcg gtgcgtggcg aggtgaccgg ccctctcgga caatccggcg ccctcgttgc
     2341 cgaacaccca ggcgaccggt cccttgaggt cgaggtcgta gagcgaagct tccgcgcgcg
     2401 gccccatggc gaccaccgag ccgcggaacg attgcgcgaa ttcgccgata tccgcaccct
     2461 cggatatcga caggaagaaa tgcgcgccca tgcctgcccg gatcactttc ggggaccacg
     2521 cgaacaccga gtctttcgaa agcaagatgt gcgtgatacc cgctgccgcc gcggtccgaa
     2581 ggatagctcc cagattcccc ggatcctgga tgttttccag caagagacag ctcgagatat
     2641 tctgcggtat cggacccggg agaggcgtac ggatcaccgc cgcgattccg gccgagcttg
     2701 cgagctgcga gacgcgttcg agaagctcgt ctgcgagaac caggcgcgat ttcgccggta
     2761 cgttttcgaa aaagtcgcgg atttccagtc tggcgagagc tgtctcgccc gcaagtacta
     2821 cctccgcgat accaccgctc tgttgatacg cgcgcaccag atgctcgcct tccaggatcg
     2881 ttgtgccggt cctgcgccgc tcccgcgacg atccggccag cttgatcagg gccttgacct
     2941 gcgggttgtc ccgggagcgg atggacttca ctcgagggcg caggtacgga atccggcgaa
     3001 gacgtcgccg cggtccggcg tgtagaaatt cctccacgtg ttggagatca gccgcgcggg
     3061 cgtcgcgaag ctgcccccgc gcaggacctt gtgggtcccg aaccagggct cggaatattc
     3121 cgcatacgga tcgcgcacga aacccggata gggtgcaaag gtgctggaag tccactccca
     3181 gacgttgccg agcatttggc ggcagccggc agcgctgtct ccttccgcga attcgcccac
     3241 gggaacgcgt cccgatccgc ccagattcgc cgctgtgacg cgcgaaaagg ctgcagccat
     3301 ctcccattcc gcttcgctcg gcaaacgccg gcccgcatag cggcaatacg cgttcgcctc
     3361 gtgccagttg acgtgcatca ccgggaggtc gccgtccaag cgcacttcct cgtcgaagcg
     3421 ccgctcgcgc cactctcggt cacgctcgac ccagtagcgc ggacctctgg cccggtcctt
     3481 ccaggtccag ccttccggcg accagagatc acgtcgctgg tagccttcgt cgcggacgaa
     3541 ttccaaaaat tgcgaattcg tgaccggcgc ccgggcaatg cggaagggat cgagcttcac
     3601 cggatgcgcc cacttttcgt tgtcgaaatg aaacccggaa tttcgcgtgg ccccgagcag
     3661 aaactctccg ccgcgcaact cgacgtctcc gtccagggcg cctttcctcg ggctgcctcg
     3721 gtcgagcggg tccgcatagc cgagtgtctg ccgtgtatag tgaaacgctt ccgcgtgcat
     3781 gtcctcatgg aagagggcca actgcacgaa atagagaaac cactcgttgt ccggctcccg
     3841 ttcgagccgc gccagcacgc gatcgagaac gcgatcctga tacgagagcg tggcggagag
     3901 ctccggcagc ggcaggtccc agcgcgtgtc gtgcgcgacc ttcgcggaat cgtagagcgc
     3961 atcggcgccc tccaggacgc tctccgagag cgattcgtcg gaccgcaatc tgaggcacca
     4021 aagctcctgg aaccaggcga cgtgccccag ctcccacagc acgggattga cgatcgccag
     4081 ctttggaccg aacaattggg ggccttcgag atcgcaggtg atcaggcggg tgcgccgcct
     4141 ggcatcgcgc aattgtcgtg acagagattc gggagacatg ggagatttaa aacatagcat
     4201 gaaaatcgcg ctcgttaccc cggcccgggc gcgctcgcga agcggcaacc gccacaccgc
     4261 gacacgctgg gccgcgatgc tgcgctccct cggtcaccgc gtgcagattt caactcgctg
     4321 ggacggccgg gccgccgacg cgatgatcgc gctgcacgcg cggcgcagcc acgactcgat
     4381 caggcgattt cgcgagcggt ttcccgcctt gcccctcgtc gtagtgctga ccggcacgga
     4441 cctctacaga gatatccgca cggatcggga cgcgcaggac tcgcttgtgc tcg
//
LOCUS       DASPZD010000217         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1766587, whole genome shotgun sequence.
ACCESSION   DASPZD010000217 DASPZD010000000
VERSION     DASPZD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766587"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VEM33_04245"
     CDS             complement(<1..820)
                     /locus_tag="VEM33_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYS51064.1"
                     /translation="MTAQVSLFNTLTRRVEPLQPLAPPRVTLYTCGPTVWNYAHIGNF
                     RTFLFEDLLRRWLEHSGYDVFQIMNLTDVDDRTIKAARAAGKPLAEHTAPFTQAFFED
                     RDYLRIKPAHVYTAATEYMPQMIALVSRLLERGVAYKGEDGSVYFALQKFPTYGRLSQ
                     LDKREIKVGARVASDEYAKGDPSDFALWKKADAEDEAVGAAWDAPFGRGRPGWHLECS
                     AMSLEEIGKCCGVQTLDIHAGGIDLIFPHHENEIAQSEGATGQTFSRHWVHGEFL"
     gene            complement(885..1757)
                     /locus_tag="VEM33_04250"
     CDS             complement(885..1757)
                     /locus_tag="VEM33_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51065.1"
                     /translation="MADYALTLSDITGRGLGPASLALLPGEIAGLVGPAGAGKTRLLR
                     VAAGAQKPRTGEVRVWGVRVTHPAARRLSGYACDSPAFPCALTVEEVLTYYARLDCAD
                     NRERSLRGLVRDALEIAGLTAATDLRADSLGRADLHRLSFAQAALGGRRVLLLDETLS
                     GLDAFARRDLRGRIARLAAEGVAVLITSRDPAVLERLAARVLVMRDGRIVRAGPLATL
                     IGERILEVILDAPPAEPPPGFRVTAAGLEASLTGRTAEAALALCRAHRLPVRASRVRV
                     KSLEEIVLETHDNN"
     gene            complement(1785..2450)
                     /locus_tag="VEM33_04255"
     CDS             complement(1785..2450)
                     /locus_tag="VEM33_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51066.1"
                     /translation="MRFLRCVHAEFLLVVPRLTRTRLGMTLLVLGTTLVWLSARGFDA
                     LTVTLQAGALGAILGATAIVGDETDRAALTTALTHSTTPLAIATGRWLAIVVPASGLV
                     IVCAAAIGSPANAVVVGVIATAAVGGCALAVVLPLGRGAALALFLFMAVAGTIAPERL
                     VDLARPGVVRLTAASAMELGPALWHYRDIATGDMGAVLHALAWTGLGILLTSEVIRRC
                     RAR"
     gene            complement(2524..>3340)
                     /locus_tag="VEM33_04260"
     CDS             complement(2524..>3340)
                     /locus_tag="VEM33_04260"
                     /inference="COORDINATES: protein motif:HMM:NF044559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51067.1"
                     /translation="PRAAISPLEREDERIVASWFVCKDKDWASEALNELVREDPDKAW
                     AVVELLVERADSDELLGSIAAGPLEDLLRKHGAMLVSRVEEKARRDLRFKRCLGGVWL
                     DPRDLSDEIIRRLHRASDGELRILEPRPTPHELLELERQAVTMLLTGAHRVLEALREQ
                     WSRSTVAHRSYGWGGGHVEFSVPADVPPITDADSVTIGDVCAEIHFLDSPVLFFLLIE
                     DGRLAGLNCTIQGDWPDPVLVKRVYWIRTDEHATQDVETSERDLNRFRRRWGE"
BASE COUNT          606 a         1134 c         1055 g          545 t
ORIGIN      
        1 tgagaaactc accgtgtacc caatggcgcg agaaggtctg gcccgtcgct ccctctgact
       61 gcgcgatttc gttttcgtga tgcgggaaaa tcaaatcgat ccccccggcg tggatgtcga
      121 gcgtctgcac gccgcagcac ttgccgattt cttcgagcga catcgccgaa cactcgaggt
      181 gccatccggg ccggccgcgg ccaaacggcg cgtcccaggc ggcgccgacg gcctcgtctt
      241 cggcatcggc ttttttccat agcgcaaaat cgctcgggtc gcccttcgcg tactcatcgc
      301 tggccacgcg cgcgccgact ttgatttcgc gcttgtcgag ctgcgatagc cggccgtagg
      361 tcggaaactt ctggagcgcg aagtagaccg agccgtcctc tcctttatat gccacgcccc
      421 gttcgagcag ccgcgacacc agcgcgatca tctgcggcat gtactccgtc gccgccgtgt
      481 agacatgtgc cggcttgatg cgcagataat cgcggtcttc gaagaacgcc tgcgtgaacg
      541 gcgcggtgtg ctcggcgagg ggcttcccgg ccgctcgcgc cgccttgatc gtgcggtcgt
      601 ccacgtcggt cagattcatg atctgaaaga cgtcgtagcc ggagtgctcc agccagcggc
      661 gcagcaagtc ttcgaacagg aacgtgcgga aattgccgat gtgcgcgtag ttccagacgg
      721 tcgggccgca ggtgtacagc gtgacgcgcg gcggcgcgag cggctgcagc ggctcgacgc
      781 gacgggtcag cgtgttgaag agcgaaactt gcgccgtcat taacggcgaa acgtagcaag
      841 gaaaggcaac tcaggtgagg ctggggtggg ggccccagac tcacctaatt gttgtcgtgc
      901 gtttccagga cgatctcctc gagtgactta acgcgaaccc tcgacgctct taccggcaaa
      961 cggtgcgccc gacacagtgc cagcgccgct tccgcagtcc gccccgtcag cgacgcctcc
     1021 agccccgcgg cggtgacccg gaatcccggc ggtggctcgg cgggcggggc atccagaatc
     1081 acctccagga tgcgctctcc gatgagcgtc gcgagcggac ccgcgcgtac gatccgcccg
     1141 tcgcgcatga ccagcacccg cgccgcgagc cgctcgagga ccgccggatc gcgcgatgtg
     1201 atcagcacgg cgacaccctc ggcagcgagc cgagcgatcc ggccgcgcag gtcacggcgt
     1261 gcaaatgcat cgaggccgga caacgtctcg tcgagcagga gcacccggcg tccccccaat
     1321 gcggcctgcg cgaagctaag gcggtgaaga tccgcccgtc ccagcgagtc cgcgcggaga
     1381 tctgtcgccg ccgtcagccc ggcgatctcg agggcgtcac gaaccaggcc gcggagcgag
     1441 cgctcgcgat tgtccgcaca atccaggcgg gcgtagtacg tcagcacttc ctcgacggtg
     1501 agtgcacacg ggaaagccgg gctgtcgcac gcgtatccgc tgagtcgccg tgctgccgga
     1561 tgggttaccc gcacccccca cacacgcacc tcgccggtcc gtggcttttg cgcgcccgcg
     1621 gccacccgca gcagccgggt ttttcctgcg cccgccggcc cgaccagccc cgcgatctcg
     1681 ccaggtagca gcgcgagcga cgcagggccc agcccgcgcc ctgtgatgtc gctcagtgtc
     1741 agggcataat cagccatgtg ccatcagccg tctgccacct gccatcagcg ggccctgcag
     1801 cgacggatga cctcgctcgt caacaggatc ccgagacctg tccatgcgag cgcatggagt
     1861 acagctccca tgtcgccggt ggcgatgtcg cggtagtgcc agagcgcggg cccgagctcc
     1921 atcgcgctcg ccgcggtcag ccgcaccacg cctgggcggg cgagatcgac gaggcgctcc
     1981 ggggcgatgg tgcccgctac cgccataaag aggaagagcg ccagcgcggc gccgcgtccc
     2041 agcgggagga ccaccgcgag tgcgcagccg ccgaccgcgg ccgtggcgat cacgcccacg
     2101 acgaccgcgt tcgcaggcga gccaatcgcc gccgcgcaca cgatcaccag ccccgaagcc
     2161 gggaccacga tcgccaacca gcgccccgta gcgatggcga gcggcgttgt ggaatgcgtc
     2221 agggcagttg tcagcgcggc gcggtcggtc tcatcgccaa cgatcgccgt cgcaccgagg
     2281 atcgcgccga gcgcgcccgc ctgtaacgtg acggtgagcg cgtcgaagcc acgcgcgctg
     2341 agccagacga gcgtcgtacc cagcaccagc agcgtcatgc ccagccgggt cctggtaagt
     2401 ctcggaacga cgagcaggaa ttcggcgtgc acgcagcgca ggaatcgcat ggcggcacga
     2461 taggcaaggg actattgggg attggtatcg aatcgttgct ctatggatct gtaaagcgcg
     2521 aacctattcg ccccacctcc tgcgaaagcg gttcagatcc cgttctgacg tctcgacgtc
     2581 ttgcgtcgcg tgctcgtccg tgcgaatcca atacactcgc ttcaccagta cggggtcggg
     2641 ccagtctcct tggatagtac agttgaggcc agcgaggcga ccgtcttcga tgagcaggaa
     2701 gaacagcacg ggactatcga gaaaatgaat ctcagcgcag acgtctccga ttgtcaccga
     2761 atccgcgtca gtgatcgggg gcacgtcagc cggaaccgaa aactcgacgt gtccgccacc
     2821 ccaaccgtaa gaacggtgcg ctactgtgga tcggctccat tgttcccgaa gtgcctcgag
     2881 cactctatgc gcacctgtaa gaagcatcgt caccgcctgg cgctctagtt ccagaagttc
     2941 gtgaggagtt gggcgcggct cgaggatccg gagctcacca tctgaggctc gatgcagccg
     3001 ccgaatgatt tcgtccgaaa gatcccgcgg atctagccaa acaccgccga gacagcgttt
     3061 gaaccgcagg tctcgtcgcg ccttttcttc cactcgggac accaacattg ccccgtgctt
     3121 tcgtaggaga tcctcgagag gaccggccgc gatgctcccg agtagctcat cggaatcggc
     3181 gcgctcgacc aggagctcga cgaccgccca tgccttgtcg ggatcttcgc gaaccaactc
     3241 gttcaacgcc tcggaagccc agtccttgtc cttgcataca aaccatgacg cgacgatgcg
     3301 ctcatcctcg cgctcaagcg gactgatggc cgcacggggg
//
LOCUS       DASPZD010000218         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1356336, whole genome shotgun sequence.
ACCESSION   DASPZD010000218 DASPZD010000000
VERSION     DASPZD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356336"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VEM33_04265"
     CDS             complement(<1..1313)
                     /locus_tag="VEM33_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYS51068.1"
                     /translation="MDRISTAQAATMPVEFKLNGKTVVGRSDETLIETAKHYGIEIPH
                     LCYKEGMRPDGNCRACVVEIKGERVLAPSCCRYPKDGMEVTTDNPRSVASQKMVLELL
                     LSDIPEKSYTLDSELDHWAKELKVGKPRFESRHQPKADLSHAAIAVNLDACIQCTRCL
                     RACREEQVNDVIGYAHRGEHSKIVFDFDDPMGDSTCVACGECVQACPTGALMPAREVG
                     KIVADKTVDSVCPYCGVGCLLTYNVKGNKILWVQGREGPANNNRLCVKGRYGFDYVQH
                     KHRLTRPLIRKPGMKKHKDFTVDPDNWSQVFREASWEEALDFAAKGLRDIRDTYGKKA
                     LAGFGSAKGTNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALMEGINSGAVSNQV
                     RDVMKAEVIFLIGANPTVNHPVAATWMKNAAKAGVKLIVADPRKG"
     gene            complement(1425..>2323)
                     /locus_tag="VEM33_04270"
     CDS             complement(1425..>2323)
                     /locus_tag="VEM33_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYS51069.1"
                     /translation="DPHRFLEGAIIAAWAIGIDDVYIYLRDEYHGCRAILHRELASLC
                     DDPPCRIPRFHLRRGAGAYIGGEESAMIESIEGKRGEPRLRPPYVAQVGLFARPTLEH
                     NMETLHWVREILERGADWFASQGRNGRKGLRSFSVSGRVKKPGVHLAPAGITVKELIA
                     EYCGGMLESHRFYGYLPGGASGGILPASMADIPLDFDTLNQYGCFIGSAAVVILSDRD
                     RATAAARNLTKFFSEESCGQCTPCRVGTAKTLKLIEKTKWDQPLLKELSQAMMDASIC
                     GLGQAAPNPVLSVMKYFPQEVT"
BASE COUNT          392 a          738 c          735 g          458 t
ORIGIN      
        1 tcgcccttgc gcggatcggc aacgatcagc ttcacgcccg ccttcgcggc gttcttcatc
       61 caggtggctg caacggggtg attgaccgtg ggattcgctc cgataaggaa gatcacctcg
      121 gctttcatca cgtcgcgcac ctgattcgac accgcgcccg agttgatgcc ttccatcagc
      181 gccgcgaccg acgaggcgtg acacaggcgc gtgcagtgat cgacgttgtt cgagccgaag
      241 ccggtgcgca ccagcttctg gaagagataa gcctcctcgt tcgtgccctt ggccgagccg
      301 aaaccggcga gcgccttctt gccgtaagtg tcgcggatgt cgcgcaggcc ttttgcggcg
      361 aaatcgagcg cttcctccca gctcgcctcg cggaacacct gcgaccagtt gtcggggtcg
      421 acggtgaagt ccttgtgctt tttcatgccg ggcttgcgga taaggggccg ggtgagccgg
      481 tgcttgtgct gtacgtagtc gaacccgtag cgccccttca cgcagaggcg gttgttgttg
      541 gcgggcccct cgcggccctg cacccagagg atcttgttgc ccttgacgtt gtaggtcagg
      601 agacatccga cgccgcaata ggggcagacc gagtccacgg tcttgtcggc gacgatcttg
      661 ccgacttcgc gcgccggcat caaagcgccg gtcgggcacg cctgcacgca ttcgccgcag
      721 gcgacgcagg tggaatcacc catgggatcg tcgaagtcga acacgatctt ggaatgctcg
      781 ccgcggtgcg cgtagccgat cacgtcgttc acctgctcct cgcgacaggc cctgaggcac
      841 cttgtgcact ggatgcaggc gtcgaggttc accgcgatcg ccgcatgcga gagatcggcc
      901 ttgggctggt ggcgcgactc gaagcgcggc ttgccgactt tcagctcctt cgcccagtga
      961 tcgagctcgg aatcgagcgt gtaggacttt tccggaatgt ccgagagcag cagctcgagc
     1021 accatcttct gactcgcgac cgagcgagga ttgtcggtgg tcacctccat gccgtccttg
     1081 gggtagcggc agcaggaggg cgcgagcacg cgctcgccct tgatctcgac cacgcaggcg
     1141 cggcagttgc cgtccggccg catgccttcc ttgtagcaga ggtgcgggat ctcgatgccg
     1201 tagtgcttgg cggtctcgat gagagtctcg tccgagcggc cgacgacggt cttgccgttc
     1261 agcttgaact caaccggcat cgtcgccgct tgggcggtgc ttatgcggtc catcggttac
     1321 ctctggccgt cacgtcgctc taaaaccggg gacagaccac cattttccgg cgcaatccct
     1381 tccgtatccc gcgttgcaaa aaaatggtgg tctgtccccg gttttcacgt cacttcctgc
     1441 gggaagtatt tcatcacaga aagcaccggg ttgggagctg cctgccccag cccgcatatg
     1501 gatgcgtcca tcatcgcctg ggaaagttcc ttcaagagcg gctggtccca tttcgttttt
     1561 tcgatgagct tcagcgtctt tgccgtgccg accctgcagg gcgtgcactg cccacaggac
     1621 tcctcggaga agaatttcgt caggttgcgc gcggcggcgg tcgcccggtc cctgtccgag
     1681 aggatcacca ctgccgccga gccgatgaag cagccgtact ggttcaaggt atcgaagtcg
     1741 agcgggatat cggccatcga tgccggcaga atcccgcccg aggccccgcc gggaagatag
     1801 ccgtagaacc ggtggctttc cagcatcccg ccgcagtatt cggcgatcag ctccttcacc
     1861 gtgatcccgg ccggcgcgag atgcacgccg ggcttcttca cgcggccgct caccgaaaaa
     1921 gagcgcaggc ccttgcggcc gttgcggccc tgcgaggcga accagtcggc gccgcgctcg
     1981 agtatctcgc gcacccagtg cagcgtctcc atgttgtgct cgagcgtggg acgggcgaac
     2041 aggccgacct gtgcgacata cggcgggcgg agtcgcggct cgccgcgctt cccctcgatg
     2101 gattcgatca tcgccgactc ttcgccgccg atgtaggcac cagcgccacg tcgaagatgg
     2161 aatcgtggaa tcctgcaggg tggatcgtcg caaaggctgg cgagctcgcg gtgcaaaatg
     2221 gcacgacagc cgtggtattc gtcgcgcaag tagatataga cgtcgtcaat gcctatcgcc
     2281 cacgcggcga tgatcgcgcc ttcgagaaaa cggtggggat cgc
//
LOCUS       DASPZD010000219         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_738952, whole genome shotgun sequence.
ACCESSION   DASPZD010000219 DASPZD010000000
VERSION     DASPZD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738952"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VEM33_04275"
     CDS             <1..525
                     /locus_tag="VEM33_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HYS51070.1"
                     /translation="AAMKELAMSGGRFEVELRSLLPDGSAAGNEQVEFLVATNPGAEP
                     RALAKVASGGELSRISLAIQVITSRAAAVPTLIFDEVDAGIGGAVAEVVGRKLKTLGG
                     ERQVLCVTHLPQVAAQAREQWSVSKASESGSARSRVAVLDQKSRIEEIARMLGGVAIT
                     ATTRKHAAEMLGLR"
     gene            569..937
                     /locus_tag="VEM33_04280"
     CDS             569..937
                     /locus_tag="VEM33_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51071.1"
                     /translation="MNAVNRQAGLSQIVLLIVIAIALAAAYVAIDLYTGGEKDMLTVE
                     TRGVGMISALSAYKRTSGTYPETLDKLVPQYVLAVSKCPNGVSMGYVVSGGEYVISCP
                     NVVFKSMPYSYDSRSKRWHG"
     gene            997..1073
                     /locus_tag="VEM33_04285"
     tRNA            997..1073
                     /locus_tag="VEM33_04285"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Val,seq:gac)
     gene            1175..>2622
                     /gene="thrS"
                     /locus_tag="VEM33_04290"
     CDS             1175..>2622
                     /gene="thrS"
                     /locus_tag="VEM33_04290"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HYS51072.1"
                     /translation="MPKIRLPDGAVKSFDGPVTVAEVAQAIGPGLARAALAGRVNGKL
                     VDTSFRLENDCELAIVTERDKEGVEILRHSTAHLLAYAVKDLFPEAQVTIGPVIENGF
                     YYDFSFKRPFTPEDLDAIERRMAELAKKDFPVTREVWPRDKAVQYFKSIGERYKAEIV
                     ALIPANEEVSLYREGDFIDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEMLQRIY
                     GTAWARKEEQEAYVRMIEEADKRDHRRLGTQLDLFHLQEEAPGMVFWHPKGWTLWQQV
                     EQYMRRIYRDNGYLEVRCPQILDRSLWEKSGHWQNYRENMFTTESEKRDYAIKPMNCP
                     GHVLIYNKGLHSYRELPLRYGEFGVCTRNEPSGALHGIMRIRAFTQDDGHIFCTEEQI
                     QPEVTAFTALLQKVYVDFGFTEVVYRLATRPPKRVGADELWEKAERALRASLDSSGLV
                     YDLLPGEGAFYGPKIEYHLKDSIGRSWQCGTM"
BASE COUNT          525 a          767 c          861 g          469 t
ORIGIN      
        1 gcggcgatga aggagctcgc gatgtccggc gggcggttcg aggtcgagct gcgctcgctt
       61 ttgcctgacg gcagcgctgc gggaaacgaa caggtcgagt ttctggtggc gaccaacccg
      121 ggcgccgagc cgcgcgccct cgccaaggtg gcctccggag gggaattgtc ccgcatcagc
      181 ctcgcgattc aggtaatcac gagccgggcg gcagcggtgc ctaccctgat tttcgacgag
      241 gtcgacgcgg ggatcggcgg agccgtggcc gaagtcgtcg gcaggaagtt gaaaaccttg
      301 ggtggggagc gtcaagtatt gtgcgtcacg catcttccgc aggtggccgc ccaggcgcgc
      361 gagcagtggt cggtgtcgaa ggcctccgaa agcggcagcg cccggagccg cgtcgcggtg
      421 ctcgaccaga aatcgcgcat cgaggaaatc gcgcgcatgc tgggcggggt cgcgatcacc
      481 gcgacgaccc gcaagcacgc ggcggagatg ctcggcttgc ggtaatattt gcgggtcagt
      541 cgacgtcggt tgaatgagga gggcagggat gaacgcggta aatcgtcagg cgggcctaag
      601 ccaaatcgtt ctgctcatcg tgatcgcgat cgctctcgcg gcagcgtatg tcgcgatcga
      661 cctctacacc gggggcgaga aagacatgct gaccgtcgag acccgtggcg tggggatgat
      721 ctccgcgctt tcggcgtaca agcgcacgag cggcacgtac ccggagacgc tcgacaagct
      781 cgtaccccag tacgtcctgg cggtgagcaa gtgtccgaac ggcgtatcca tgggctacgt
      841 ggtctcgggc ggcgagtacg tgatttcctg tccgaacgtg gtcttcaaat ctatgccgta
      901 cagctacgat tcgcgatcga agcgttggca tggttgatcg tcgtgtttcg cgggtttgtg
      961 cgggcgggac cgttcggcta gaatccggca cttcccaggc gcgtagctca gttggctaga
     1021 gcgcatcctt gacatggatg aggtcgttgg ttcgattcca atcgcgccta ccaactgtta
     1081 aaacggcagc ctcgatggct gccgttttag ctctgcgcgt cggaacggac gcgatgcgcc
     1141 ggtcaacgcg cagacggata tagaaaacac gctgatgccc aagattcgtc tgccagatgg
     1201 cgccgtcaag tcattcgacg gcccggtcac tgttgccgaa gtcgcccagg cgatcggacc
     1261 aggactggcg cgcgccgcgc tcgcgggaag ggtgaacgga aaattggtcg atacctcttt
     1321 ccgtctcgag aacgattgcg agctcgccat agtgaccgaa cgcgacaagg agggcgttga
     1381 gatcctgcgg cactccaccg cgcacctgct cgcctacgcc gtgaaggacc tgtttcccga
     1441 agcgcaagtg accatcgggc cggtgatcga aaacggcttc tactacgatt tttcgttcaa
     1501 gagaccgttc acgccggaag atctcgatgc catcgaaagg cgcatggcgg aactggcgaa
     1561 aaaggatttc ccggtgacgc gcgaggtctg gccgcgcgac aaggcggttc aatacttcaa
     1621 atccatcggc gagcgctaca aggcggagat cgtcgcgttg attcccgcga acgaagaggt
     1681 ctcgctgtat cgggaagggg atttcatcga tttgtgccgc ggcccgcacg ttccttccac
     1741 cggcaagctc aaggtattca agctgatgaa ggtcgccggc gcctactggc gcggagattc
     1801 caagaacgag atgctgcagc ggatctacgg gaccgcctgg gccaggaagg aagagcagga
     1861 agcgtacgtg cgcatgatcg aggaggcgga caagcgcgac caccggcggt tgggcacgca
     1921 actggatctc ttccatcttc aggaggaagc cccgggcatg gtgttttggc atccgaaagg
     1981 ctggacgctc tggcagcagg tggagcagta catgcgtcgc atctaccggg acaacggcta
     2041 tctcgaagtg cgctgccctc agatcctcga ccgcagcctg tgggagaaat cgggccactg
     2101 gcagaactac cgggaaaaca tgttcaccac cgagtcggag aagcgcgact acgcgatcaa
     2161 gccgatgaac tgtcccgggc acgtactgat ctacaacaag ggcctgcaca gctatcgcga
     2221 gctcccgctg cgctacggcg agttcggcgt gtgcacgcgc aacgaaccct cgggcgctct
     2281 gcacggcatc atgcgcatcc gcgccttcac ccaggacgac ggccacatat tttgcaccga
     2341 ggagcagatc cagccggagg tcacggcgtt tacggcgttg ctgcaaaagg tctacgtgga
     2401 cttcggcttc accgaggtcg tctacaggct cgcgacgcgc cctccgaaaa gagtcggggc
     2461 agacgagctt tgggaaaagg ccgagcgcgc gcttcgggca tccctcgact cgagcggcct
     2521 cgtctacgac ctgctcccgg gggagggcgc attttacggt cccaagatcg aataccatct
     2581 gaaagacagc atcggccgct cttggcaatg cggcaccatg ca
//
LOCUS       DASPZD010000220         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_23723, whole genome shotgun sequence.
ACCESSION   DASPZD010000220 DASPZD010000000
VERSION     DASPZD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23723"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VEM33_04295"
     CDS             <1..1152
                     /locus_tag="VEM33_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS51073.1"
                     /translation="IAWGTVAVVVLLAFGGGLERQTKKRFHGLGDRIVILFGGRTTKP
                     FAGFGDGRPIRLRPDDVELLAREVAEIEFISPEFITQSARVRRGVKAASPAVTGILPV
                     YGFMRNIIAEAGGRFINERDVAERRRVAVLGDELAKLLFGDAAPVGQQVFLGDTPFTV
                     VGVMRPKLQNSSYQQRDKDRIFIPATTHYALFGNRFLNNIVYRARSSEVTKLAERRVY
                     ETMARTYKFDPLDKDALGFWDTSEWETRFEKMFVAFNVFFAIVGSFTLLVGGIGVANI
                     MYIVVRERTREIGVRRAVGATRTDILRQFFAEALMIVTMGAMLGLLLSFGLVAALGGL
                     PIKEFVGVPTISPMVLAATLALLAAVAFAAGLMPARRAAALDPVEALRT"
     gene            1155..>2274
                     /locus_tag="VEM33_04300"
     CDS             1155..>2274
                     /locus_tag="VEM33_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS51074.1"
                     /translation="MFYILLQEFIGDLKAQRTRAFLTFFAVTWGTLAVVLLLAFGEGL
                     KRTVRDGLLGAGERIFMIYGGETSKAFAGLGQGRRIRLVEEDLELLKGAIPDIDRGSL
                     SYGRWGTSFQLGKTRTNAFLEGVGPDHEPMRHMEPAQGGRFINALDIAQKRRVLFLGN
                     ELAERVFGKGVDAIGKSVVVDGLPFIVVGVLKKKFQDSSNNGPDADRGVIPYSTFRTI
                     YGNRFVNHLIVQPRDVSRGPLVKGELYRVLGQKYRFDPKDERALGMWDFIEDEKQTLA
                     VGLGIQIFMGLVGVFTLLIAGVGVANIMYVVVKERTQEIGIKLALGARRRHIMAQFLF
                     EAMTIAVSGGLVGLAAAALIVLGVDALPKEDNPAMQYIL"
BASE COUNT          369 a          707 c          793 g          405 t
ORIGIN      
        1 atcgcctggg gcacggtggc ggtggtggta ctgctggcgt tcggtggcgg tctagagcgg
       61 caaaccaaga agcggtttca cggcttgggc gaccgcatcg tgattctgtt cggcggacgc
      121 accaccaagc cgttcgcggg gttcggcgac ggccgcccga tccgcctgcg gccggatgac
      181 gtggagctgc tcgcccgcga ggtcgccgaa atcgagttca tcagtccgga gttcatcaca
      241 cagagcgcgc gggtgcggcg cggcgtcaaa gcggcctccc ccgccgtcac gggcattctc
      301 ccagtgtacg gcttcatgcg caacatcatc gcggaggcgg gcggtcgttt catcaacgag
      361 cgcgacgtcg ccgagcggcg gcgggtggcc gtgctgggcg acgagctcgc taagctgctg
      421 tttggtgacg cagcgccggt cggccagcag gtgttcttgg gcgacacgcc gttcaccgtc
      481 gtgggggtga tgcgccccaa gctgcagaac tcctcgtatc agcagcggga caaggatcgg
      541 atcttcattc cggcgacgac gcattacgcc ctgttcggca accgcttcct gaacaacatc
      601 gtgtaccgcg cgcggagctc ggaggtcacc aagctggccg agcgacgggt gtacgagacg
      661 atggcgcgca cgtacaagtt cgatccgctg gacaaggacg ccctcggctt ctgggatacg
      721 agcgagtggg agaccaggtt cgagaagatg ttcgtcgcct tcaacgtttt cttcgccatc
      781 gtcggaagct tcaccctgct cgtaggcggc atcggggtgg cgaacatcat gtacatcgta
      841 gtgcgggaac ggacgcgcga aatcggcgtc cggcgcgccg tgggcgccac gcgcaccgac
      901 atcctgcgcc agttcttcgc cgaggccctg atgatcgtga ccatgggagc gatgctcggc
      961 ctgctgctct cgttcgggct cgtggccgcg ctcggcgggc tgcccatcaa agagttcgtc
     1021 ggcgtgccga cgatttctcc gatggtactg gcggcgacgc tcgcgttgct cgcggccgtg
     1081 gcgtttgccg ccggcctcat gccggcccgg cgcgcggcgg cgctcgatcc cgtggaagcc
     1141 ctgcgaacct aggcatgttc tacatcctgc tccaggagtt catcggtgac ctgaaggcgc
     1201 aacgcacccg ggcgttcctt acgttcttcg ctgtcacctg gggaacgctc gcggtggtgc
     1261 tgctgctggc gttcggggag ggactgaagc gcacggtccg cgacggcctg ctgggcgccg
     1321 gcgagcggat cttcatgatc tacggcggcg agaccagcaa ggcattcgcc gggttgggcc
     1381 aagggcgacg catccgcctc gtggaagagg acctcgagct cctgaagggt gccatcccgg
     1441 atatcgatcg ggggagcctg tcgtacgggc ggtggggtac ctcctttcag ctgggcaaga
     1501 cccgcaccaa cgcgttcctc gagggcgtcg gtcccgatca cgagcccatg cgccacatgg
     1561 agccggcgca gggcgggcgc ttcatcaacg ccctcgacat cgcccagaag cggcgcgtgt
     1621 tgttcctcgg caacgagctg gcagagcggg ttttcggcaa aggcgtcgat gccatcggca
     1681 agagcgtcgt cgtcgatggc ctccccttca tcgtggtcgg agtgctgaag aagaagttcc
     1741 aggactcgag caacaacggg ccggacgccg accgcggggt gatcccgtac tcgacgtttc
     1801 gcaccatcta tggcaaccgg ttcgtgaacc acctcatcgt gcagccgcgc gacgtgtccc
     1861 ggggacctct ggtgaaggga gagctctacc gggtgctggg gcagaagtac cggttcgacc
     1921 ccaaggatga gcgggcgctg ggcatgtggg acttcatcga agacgagaag caaaccctcg
     1981 ccgtcgggct cggcatccag atcttcatgg ggctcgtcgg ggtgttcacg ctgctgatcg
     2041 cgggcgtcgg ggtcgcgaac atcatgtacg tcgtggtgaa ggagcggacg caggagatcg
     2101 gcatcaagct cgcgctcggc gcccgccgcc ggcacatcat ggcgcaattc ctgttcgagg
     2161 ccatgacgat cgccgtgtcc ggcgggctgg tgggactcgc ggcggcggcg ctcatcgtgc
     2221 tcggggtgga cgcgctcccg aaggaagaca atccggcgat gcagtatatc ctca
//
LOCUS       DASPZD010000221         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_803201, whole genome shotgun sequence.
ACCESSION   DASPZD010000221 DASPZD010000000
VERSION     DASPZD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803201"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VEM33_04305"
     CDS             <1..1449
                     /locus_tag="VEM33_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYS51075.1"
                     /translation="RIGLERDILDDAQLLLEAVLDLAPDYHAARFDYAQVLIKRHMYP
                     QAHEQAEKLLAVDRANRNYRTLYAMTSVGLGLHERAIELYRELLAGATQPADLHLSIA
                     HSLKTLGRREEAIAAYRAAAAARTGYGDAWWSLANLKTYRFADGELERMRAAESAAAT
                     SVVDRYHLCFALGKALEDRQQYEKSFEYYARGNALKRSESRYRPQVFELNTCLQIEVC
                     TAELFARNKDGGAHAADPIFIVGLPRSGSTLIEQILASHSAVEGTQELADLPRIVVDL
                     QGRDWSFDNPRYPAVLAQMSAEDFHRLAEKYLSDTRVYRTGKARFIDKMPNNFRHVGL
                     IHLMLPNAKIIDARREPMACCFGNLKQLFAQGQEFTYSIEDIARYYRTYLELMRHWDA
                     VLPGRVLRMQHEDVVDDLEGNVRRLLDYCGLEFEPACMEFHKTERSVRTASSEQVRQP
                     IYREGLDQWRHYERWLGSLREALGDALTRYRD"
     gene            complement(1430..>2091)
                     /locus_tag="VEM33_04310"
     CDS             complement(1430..>2091)
                     /locus_tag="VEM33_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS51076.1"
                     /translation="DAHERTLGFREIFLGYVLPNRYLWAIAVANAFCYFVRYGVVNWI
                     PTYLETAKGFSFQQSSIGWSLYEWAAIPGTIACGWISDKWFQGKRAPATMLFMALTLV
                     AVLVYWLNGKGPLWIDYAALVAIGFLIYGPIMIIGLHSLDLVPKKAAGTAAGFTGFFG
                     YVFGSAISGTGVGWIADRWGWGGVFTTMVACCLLTIFFSALTLGHQARSEGRLPSLGS
                     A"
BASE COUNT          408 a          677 c          660 g          346 t
ORIGIN      
        1 cgcatcggcc tcgagcggga catactcgac gatgcgcaac tgttgctgga ggcggtgctc
       61 gatctggcgc ctgattatca cgcggctcgg tttgattatg cccaggtcct catcaagcgc
      121 cacatgtacc cacaggcgca cgagcaagcc gagaagctgc ttgccgtgga tcgtgccaat
      181 cgcaactacc gcacgctcta tgcgatgacc agcgtgggtc ttggcctgca cgaacgcgcc
      241 atcgagctgt accgggaact gttggccggc gccacgcagc cggcggatct gcatctgtcg
      301 atcgcgcact cgctcaagac gctcgggcgt cgcgaggaag ccatcgccgc gtaccgcgcc
      361 gcggcggccg cgcgcaccgg ctatggcgac gcctggtgga gtctcgcgaa tctcaagacc
      421 taccgattcg cagacgggga gctggagcgg atgcgggctg cggaatcggc ggcggcgacg
      481 tccgtggttg atcgatacca cctgtgcttt gccctcggta aagcgctcga ggaccggcag
      541 cagtacgaga aatcctttga gtactacgcc cgcggcaacg ccctcaagcg ctccgaaagc
      601 cgctatcgac cgcaggtctt cgaactcaac acctgcctgc agatcgaagt ctgtaccgcg
      661 gagctgttcg cccgtaataa ggatggcggc gcgcacgccg ctgatccgat attcatcgtg
      721 ggtctgccgc gctccggctc gacactgatc gagcagatcc tggcgtcaca ctccgcggtc
      781 gaaggcaccc aggagctcgc cgatctcccg cgcatcgtcg tggacctgca gggccgtgac
      841 tggagcttcg acaacccgcg ctatccggcg gtgctggcgc agatgagtgc cgaggatttt
      901 caccgcctcg ccgagaagta cttgagcgat acgcgtgtct accgcaccgg caaggcgcgc
      961 ttcatcgaca agatgccgaa caacttccgg cacgtcggac tcatccatct gatgttgccc
     1021 aacgcgaaga tcatcgacgc acggcgcgag ccgatggcct gttgtttcgg taacctcaag
     1081 cagctgttcg cgcagggcca ggaatttacc tacagcatcg aagacatcgc gcgctactac
     1141 cgcacctacc tggagctcat gcgccactgg gatgccgtcc tgcctgggcg cgtgctgcgc
     1201 atgcagcacg aggacgtggt ggatgacctg gaaggcaacg ttcgtcgctt actcgactat
     1261 tgcgggctcg agttcgagcc cgcctgcatg gagttccaca aaaccgaacg cagcgtgcgt
     1321 accgcgagtt cggagcaggt ccgccagccg atctatcgcg agggtctgga ccaatggcgt
     1381 cactacgaac ggtggctggg gagcctgcgc gaggccctcg gcgatgccct cacgcgctac
     1441 cgagactagg gaggcggcct tcgctccgcg cctggtggcc gagggtgaga gcgctgaaga
     1501 agatcgtcag caggcagcag gccaccatgg tcgtgaacac gccgccccag ccccagcggt
     1561 cggcgatcca gccgacgccg gtcccggaga tggccgagcc gaacacgtag ccgaagaaac
     1621 cggtgaagcc cgccgcggtg ccggcggctt tcttcggcac cagatccagc gagtgcaggc
     1681 cgatgatcat gatcggaccg tagatgagga agccgatcgc aaccagcgcg gcataatcga
     1741 tccatagcgg acccttgccg ttgagccagt agaccaggac cgcgacgagt gtcagggcca
     1801 tgaataacat ggtggccggt gcacgcttgc cctggaacca cttgtccgag atccagccgc
     1861 acgcgatcgt cccgggaatc gccgcccact cgtacagcga ccagccgatg ctcgactgtt
     1921 ggaacgagaa gcccttggcg gtctcgaggt acgtcgggat ccagttcacc acgccgtagc
     1981 gcacgaagta gcagaacgca ttggccaccg cgatcgccca caggtagcga ttcggcagaa
     2041 cgtagccaag gaagatctcg cggaacccga gggtacgctc gtgcgcgtcg c
//
LOCUS       DASPZD010000222         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1456277, whole genome shotgun sequence.
ACCESSION   DASPZD010000222 DASPZD010000000
VERSION     DASPZD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456277"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VEM33_04315"
     CDS             <1..320
                     /locus_tag="VEM33_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51077.1"
                     /translation="VALAVTSSKPDPALPGIPTVAEAGVPGYEAVEWQGIVVPSGTPG
                     PVIDRLNRAIVESVKAPEFNERLAGVGAHAVGSTPGEFADYIKKELATWSKVIKATGI
                     RIE"
     gene            313..1497
                     /locus_tag="VEM33_04320"
     CDS             313..1497
                     /locus_tag="VEM33_04320"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYS51078.1"
                     /translation="MSSSSKDRTMPSRGVWIEQFPGNLTWSNAALVTKGMAPYGAVAL
                     GEIDEVCEKLKARQGDADAWREEWCAMAERLERAADAAAAEGRQLTAGNAYLRAGMYY
                     FTGERFVVPGEQKREIGRKALQCQHAGLERRYPNIERVEVPYENTTLPALFMKAPGAS
                     APAPTVVVFDGMDNCKEMSVLFAGLEFATRGWNTLAIDGPGQGESLRLRGLHARHDYE
                     VPGTAAYEYLAGRREVDPQKVVIMGYSFGGYYAARIAAFEKRYCAGVAMSALHWDLAA
                     WQREVKRRHETDPKTTPQSSFHFRWIMGAEDIDAAIERAERFSLAGVAHKIACPFLVV
                     HAEDDKVVPVASAHKLFDAIGASRKSLKILTAEDGSTYHAQADNRQVGIDCIADWIVA
                     NL"
     gene            1536..>2028
                     /locus_tag="VEM33_04325"
     CDS             1536..>2028
                     /locus_tag="VEM33_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYS51079.1"
                     /translation="MDVSSPVLKQISTESEAPPRVAPKRKAWKGAEILVEYLIKEKVP
                     YLFGVCGHGNIGFLDAAADAASRIKTISVHHEQAAGYMADAYYKVRHEPVATFTSCGP
                     GSCNLPVALAGAMMDSSAFLAITGNVPTTQFNRMPFQETGRYFQGDFPSVIRPLVKKS
                     FQPT"
BASE COUNT          389 a          655 c          672 g          312 t
ORIGIN      
        1 tggtggccct tgccgtgacg agctcgaagc ccgatcccgc gcttcccgga ataccgaccg
       61 tggccgaggc cggcgtaccg ggctacgaag ccgtggaatg gcaaggcatc gtcgtgcctt
      121 cgggcacgcc cggcccggtc atcgaccggc tcaatcgggc gatcgtcgag tcggtaaagg
      181 cgcccgagtt caacgaacgc ctcgcaggcg tgggcgcgca tgcggtgggc agcacaccgg
      241 gcgagtttgc cgattacatc aagaaggagc tggccacatg gtcgaaggtc atcaaggcga
      301 cgggaatacg gattgagtag cagcagcaag gatcgcacga tgccgtcgcg cggcgtctgg
      361 atcgagcagt tccccggcaa cctcacctgg tcgaacgcgg ctctcgtcac gaaggggatg
      421 gcgccttacg gcgcggtcgc gctcggagag atcgacgaag tctgcgaaaa actgaaagcc
      481 cgccagggcg acgccgacgc ctggcgggag gagtggtgcg cgatggcgga acgcctcgag
      541 cgcgcggccg acgcggccgc ggccgagggg cgccagctca ccgcgggcaa cgcctacctg
      601 cgcgccggaa tgtattactt caccggggag cgcttcgtcg tcccgggcga gcaaaaacgc
      661 gagatcggcc gcaaagccct tcaatgccag cacgccgggc tcgagcggcg ctacccgaac
      721 atcgagcgcg tcgaagtgcc gtacgaaaat accacgctgc ccgcgctctt catgaaagcc
      781 ccgggcgcgt ccgcacccgc gccgacggtg gtcgtattcg acggcatgga caactgcaag
      841 gagatgagcg tgctgttcgc gggactggag ttcgcaacgc gcggctggaa tacgctcgcc
      901 atcgacgggc ccggacaagg cgagtcgctg cgtcttcgcg ggctccatgc acgccacgac
      961 tacgaagttc ccggcacggc ggcctacgaa tacctcgccg gacggcgcga ggtcgatccg
     1021 cagaaagtgg tgatcatggg ctacagcttc ggcggctact acgctgcccg catcgccgca
     1081 ttcgagaagc gctattgtgc gggcgtggcg atgagcgcgc tgcactggga ccttgcagca
     1141 tggcaacgcg aagtcaagcg caggcacgag accgacccga agacgacccc gcaatcgagc
     1201 tttcacttcc gctggatcat gggggccgag gacatcgacg cggcgatcga gcgggccgag
     1261 cgcttctcgc tcgcgggagt cgcgcacaag atcgcctgcc cgtttctcgt cgtgcacgcc
     1321 gaggacgaca aggtggtgcc cgtcgcctcc gcccacaagc tcttcgatgc gatcggcgcc
     1381 agtcgcaaga gcctgaagat tctgaccgcc gaggacggga gcacgtatca tgcgcaagcc
     1441 gacaaccggc aagtcggaat cgattgcatc gccgactgga tagtcgcaaa cctgtagcgg
     1501 tagaatcgct cgctgagcta cgaaggagag cagtcatgga tgtgtccagc ccggttctca
     1561 agcagatctc gacggagagc gaggcgccgc cgcgcgttgc gccgaagcgc aaagcctgga
     1621 agggcgccga gatcctggtc gaatacctga tcaaggagaa agtcccttac ctgttcggcg
     1681 tgtgcgggca cggcaacatc ggttttctcg acgccgcggc cgatgccgcc tcccgcatca
     1741 agacgatctc ggtgcaccac gagcaggcgg cgggctacat ggccgatgcg tactacaagg
     1801 tccgccatga gccggtggcg accttcacct cgtgcggtcc gggatcgtgc aatcttccgg
     1861 tcgcgctcgc gggggccatg atggattcgt ccgcgtttct cgcgatcacc ggcaacgtgc
     1921 ccaccaccca gttcaaccgc atgccgttcc aggaaaccgg ccgctatttc cagggcgatt
     1981 tcccctcggt catccgcccg ctggtgaaga agagctttca gccgacgc
//
LOCUS       DASPZD010000223         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1376066, whole genome shotgun sequence.
ACCESSION   DASPZD010000223 DASPZD010000000
VERSION     DASPZD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376066"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEM33_04330"
     CDS             <1..392
                     /locus_tag="VEM33_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HYS51080.1"
                     /translation="MPVETLPAASWHTSLGLGFVSAVFDNIPLTALALKQGGYDWGYL
                     AYAVGFGGSMIWFGSSAGVALSSMFPESRSVGRWLYHGWHVSLAYVLGFFTLLIVMGW
                     HPNPPHKAGVVPNSAPAASAPAGVARP"
     gene            574..1728
                     /locus_tag="VEM33_04335"
     CDS             574..1728
                     /locus_tag="VEM33_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYS51081.1"
                     /translation="MSKEQSKKSETASRRKFLAGAAAATAGAATLGFPMIAKGQAGPI
                     SMRWQSTWPSKDIFHEYALDFAKKVNDMTGGDLRIEVLPAGSVVPAFGLLDAVSKGTL
                     DGGHGVLVYHYGKQNALALWGSSPAYAMDANMLLSWHKYGGGKELLNKLYASIGANVV
                     SFPYGPMPTQPLGWFKKPITKPDDFKGMKYRTVGISIDLFTGMGAAVNALPGGEIVPA
                     IDRGLLDAAEFNNATSDRILGFSDVSKICMLQSYHQNGEQFEITFNKTKYDALPGKMQ
                     AIIANAVEAASADMSWKAINRYSQDYIELQTKDKVRFYKTPDSVLQKQLEIFDQVDKK
                     KAAENPMWKEITESQKKYAERTVRWYLDTQVGARMAYNYYFGKKPAPKKA"
     gene            1853..2476
                     /locus_tag="VEM33_04340"
     CDS             1853..2476
                     /locus_tag="VEM33_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HYS51082.1"
                     /translation="MQKLLLFVDKISTWVGQAFSWFIVALTLHVSWEVFSRYVLDHPR
                     AWAFDAMIMMYGTLFMMAGAYTLAKNGHVRGDVIYGFFTPRAQAALDLTLYIVFFIPG
                     VIALVWAGYTYAAESWAINEHSNITSEGPPVYPFKTVIPLAGAFVLVQGIVEIIRCVI
                     CLKEGDWPSREADVEEVDVDKLKQELHVKDEDIEKLDKFVVQQERGK"
     gene            2479..>2804
                     /locus_tag="VEM33_04345"
     CDS             2479..>2804
                     /locus_tag="VEM33_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HYS51083.1"
                     /translation="MRKELWFGFSLMAAIIIPVVVLMPWGHLTNGHLGLMMLALICVG
                     IMLGFPTAFTLMGMGVFFAWLAYRSVDPATADRRVLDLFVQRTYGVMSNDVLIAIPLF
                     LFMGYLV"
BASE COUNT          543 a          853 c          884 g          524 t
ORIGIN      
        1 tgatgccggt cgagacgctg cccgcggcgt cgtggcatac ctcgttggga ctggggttcg
       61 tctccgccgt attcgacaat atcccgctca cggccctcgc gctgaagcag ggcggctacg
      121 attggggcta cctggcctac gctgtgggct tcggcgggtc catgatctgg ttcggctcga
      181 gcgccggcgt tgcgctctcc agcatgtttc cggaatcgcg gtcggtgggg cgctggctct
      241 atcacgggtg gcacgtgagc cttgcctacg tgctcggctt cttcaccttg ctgatcgtca
      301 tgggctggca tccgaatccg ccccataagg cgggagtggt tccgaacagc gcgccagcgg
      361 cgagcgcccc agcaggagta gcaaggccct gaaacggctt gatttcgagg ccttatagga
      421 atacgattcg cgggctttgg cgtgtgcaag ccccagggcc ccggggggaa caatcgggcc
      481 ccacgaatcg tttaccgcag aaggttccgg ggaacaagaa gaacgacgcc gggacggcca
      541 tccatgaacc gccaccatcc gaaggaggag ctaatgagca aggaacaatc caagaaatcc
      601 gaaaccgctt cgcgccgcaa attcctggca ggtgccgcgg cggcgactgc cggcgccgca
      661 acgctgggct ttcccatgat cgccaagggc caggccggcc cgatcagcat gcgctggcag
      721 tcgacctggc cgtcgaaaga catcttccac gaatacgccc tcgatttcgc gaagaaggtg
      781 aacgacatga ccggcggtga tctcaggatc gaagtgcttc cggcgggatc ggtggtgccg
      841 gccttcggcc tgctcgacgc ggtgtcgaag gggacgctcg acggcggcca cggcgtgctc
      901 gtctaccact acggcaagca gaacgccctg gcgctctggg gctcgagccc cgcgtatgcg
      961 atggacgcca acatgctgct ctcctggcac aagtacggcg gcggcaagga actgctcaac
     1021 aagctctacg cctcgatcgg cgcgaacgtg gtctccttcc cctacgggcc gatgccgacg
     1081 cagccgctcg gatggttcaa gaagccgatc accaagccgg acgatttcaa gggcatgaag
     1141 taccgcaccg tgggcatctc gatcgacctg ttcaccggca tgggcgcggc ggtgaacgcg
     1201 ctgcccggcg gcgagatcgt cccggcgatc gaccgggggt tgctcgacgc agcggagttc
     1261 aacaacgcga cctccgaccg catcctcggt ttctccgacg tgtccaagat ctgcatgctg
     1321 cagagctacc accagaacgg cgagcagttc gagatcacct tcaacaagac caagtacgac
     1381 gcgctgcccg gcaagatgca ggcgatcatc gcaaacgcgg tggaggcggc atccgcggac
     1441 atgtcgtgga aggcgatcaa ccgctactcg caggactaca tcgagctgca gaccaaggac
     1501 aaggtgaggt tttacaagac cccggactcg gtgctgcaga agcagctcga aatcttcgac
     1561 caggtcgaca agaagaaggc ggccgagaac ccgatgtgga aggagatcac cgaatcccag
     1621 aaaaaatatg ccgagaggac ggtgcgctgg tacctcgaca cgcaggtcgg cgcgcgcatg
     1681 gcgtacaact actacttcgg caagaaaccg gcgccaaaaa aggcctgacg gcgaacacgc
     1741 tcgtgggaca ccatccggcc gaccggatgg tgtttttttt gatcctcgtc agagttgcga
     1801 aaggatgggc ggaatagact tcctcccgcc ctatcgcctc aggatttcag ccatgcagaa
     1861 actgctgctt ttcgtcgaca agataagcac ctgggtgggg caggcgtttt cctggttcat
     1921 cgtggcgctc accctgcacg tcagctggga agtgttttcg cgctacgttc tagaccaccc
     1981 gcgcgcctgg gccttcgacg cgatgatcat gatgtacggg acgctcttca tgatggccgg
     2041 cgcctatacg ctggcgaaga acggccacgt gcgcggggac gtgatctacg gtttcttcac
     2101 gccgcgggcg caggcggcgc tcgacctgac gctgtacatc gtgttcttca ttccgggcgt
     2161 cattgcgctc gtgtgggccg gctacaccta cgccgccgag tcctgggcga tcaacgagca
     2221 ttcgaacatc acctccgaag gcccaccggt ttatcccttc aagacggtca tcccgctcgc
     2281 cggagcgttc gtgctggtgc agggcatcgt cgagatcatt cgctgcgtga tctgcctgaa
     2341 agagggcgat tggccttcgc gcgaggcgga cgtcgaggaa gtcgacgtcg acaagctgaa
     2401 gcaagaactg catgtcaagg acgaagacat cgagaagctc gataagttcg tcgtgcagca
     2461 ggagcggggc aagtgaagtt gcgcaaggag ctctggttcg gcttttcgct gatggcggcc
     2521 atcatcattc cggtggtcgt gctcatgccg tggggacacc taacgaacgg acatttgggc
     2581 cttatgatgc tcgcgctgat ctgcgtgggc atcatgctcg gctttcccac cgctttcacg
     2641 ctcatgggca tgggcgtgtt cttcgcctgg ctcgcctatc gcagcgtcga tccggcgact
     2701 gcggaccggc gcgtgctcga cctcttcgtg cagcgcactt acggcgtgat gtcgaacgac
     2761 gtgttgatcg cgatcccgct gttcctgttc atgggctacc tcgt
//
LOCUS       DASPZD010000224         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1223608, whole genome shotgun sequence.
ACCESSION   DASPZD010000224 DASPZD010000000
VERSION     DASPZD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223608"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1495
                     /locus_tag="VEM33_04350"
     CDS             191..1495
                     /locus_tag="VEM33_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HYS51084.1"
                     /translation="MRTLVSIAFVALAAPLSSLAAQSSGPKAPIGAYTFVPELPGMGR
                     PVDITAVAARRQALMKRIGRGTVLIPAGHERNVETDYIQDNDFRQDNTFYYFTELETQ
                     DAVLMMTARGPDSIETILFLPPRTPSQERWTGLRLGPDSVAVRLSGITKVLPLDSLDA
                     RVRAAPQPVYSPRPRQSDASANNLTPIVDSMRAVKDADEIMRLRRAVDISVAGHVAAM
                     RAARPGMYEYQLEAVLEEGFRSNGADRVGYPSIVGSGPNSTTLHYDVNRRRTENGDLV
                     VIDAAAEWGQYTADVTRTFPINGRFTPRQKAIYDLVLGAQQAAFDALRPGMTMRELDG
                     VARKYMRDHSGNLCGERTCDDREFFNHGLGHPIGMDVHDVGISRPLEPGMVVTLEPGI
                     YIQAEKLGVRIEDDVLVTARGGEWLSAGAPRTTDAIEKLMGR"
     gene            complement(1496..2281)
                     /locus_tag="VEM33_04355"
     CDS             complement(1496..2281)
                     /locus_tag="VEM33_04355"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS51085.1"
                     /translation="MRLSLCLLAPLFGATLAAQTAAQHVALGDSAGLMHPDIQLRHYQ
                     AALAVDSLSYAANWKAARAIADVAKQIQGNADSLKRRRDSLYSVGRGYAERAIRADST
                     RPDGHYALSMVLGRLSRTKGSKERVRYAKIIYDEATRAVQIDSTYDNAHHVLGAWNAE
                     VKRLSGFQRFFAKALFGGGFMDKANWDDAVMHLQTAVRLAPDHIYHRLELAEVYVDLG
                     KYSLARVQLQAIAALPPVDVLDPQYKEDAAALFKDIEKEKDET"
BASE COUNT          412 a          761 c          815 g          418 t
ORIGIN      
        1 tgtaaggaac tggaggcttc cttcagaatc tcgatgacgg cgaggaggtg cttgcgctca
       61 tgctcgagct cggcgatccg cgcggcgagc cgttctgctt ccgcgtgtgt cagggccgag
      121 ttgatcgtca tgagtgccat agtttcgctc agggactcgg agtccgtcaa gcgcgtatag
      181 attacgcctg atgcgcacac tcgtatcgat cgctttcgtc gctctcgcgg ctccgctctc
      241 gtctctggcc gcgcagtcgt caggtcccaa ggcgcccatc ggggcgtaca cgttcgttcc
      301 cgagctgccg ggcatgggcc ggcccgtgga catcacggcc gtcgccgcgc gccgccaggc
      361 gctgatgaag cgcatcggcc gcggcacggt gctgattccc gctgggcacg agcgcaatgt
      421 cgagaccgac tacatccagg acaacgattt tcggcaggac aacaccttct actacttcac
      481 ggagctcgag acgcaggacg cggtgctcat gatgaccgcc cgcggtccgg attcgatcga
      541 aacgatcctg ttcttgccgc cccggacgcc gtcgcaggaa cgctggaccg ggttgcggct
      601 cggtcctgac agtgtggcgg tgcggctctc cggcatcacg aaggttctgc cgctcgattc
      661 gctcgacgct cgcgtgcgcg cggctcccca gcctgtttac agcccgcgac cacgccagtc
      721 ggacgcttcg gccaacaacc tgacgccaat cgtcgactcc atgcgggctg tgaaggatgc
      781 ggacgagatc atgcgcctgc ggcgcgcggt ggacatcagc gtcgcggggc acgtcgcggc
      841 gatgcgggcc gcgcggccgg ggatgtacga gtaccagctc gaggcggtgc tggaagaagg
      901 attccggagc aacggcgccg accgcgtagg ctacccgtcc attgtgggga gcggtcccaa
      961 ttccacgacg ttgcattacg acgtgaaccg ccggcgcacc gagaacggcg atctcgtcgt
     1021 gatcgacgcc gcggccgagt gggggcagta caccgccgac gtcacgcgga ctttcccgat
     1081 caacggcagg ttcacgccgc gccagaaggc gatttacgat ctggtgctcg gggcgcagca
     1141 ggcggccttt gatgccctcc ggccggggat gacgatgcgc gagctggacg gcgtcgcgcg
     1201 gaaatacatg cgcgaccact ctgggaatct ctgcggcgag cgcacctgcg atgaccgcga
     1261 gttcttcaat cacgggctcg gtcaccccat cgggatggat gtgcacgacg tcgggataag
     1321 ccggccgctc gagccgggga tggtggtcac gctcgagccg gggatctata tccaggcgga
     1381 aaagctgggc gtgcgaatcg aggacgacgt gctagtcacg gccaggggcg gggaatggct
     1441 gtcggcgggg gcgccgcgca ccaccgatgc gatcgagaaa ctgatggggc ggtagttagg
     1501 tctcgtcctt ctctttctcg atgtccttga aaagcgccgc cgcgtcttcc ttgtactgcg
     1561 gatcgagcac gtccacgggc ggcagcgccg cgatcgcctg cagctgcacc cgcgccagcg
     1621 aatacttacc cagatcgacg tagacctcgg ccagctcgag gcggtggtag atgtgatcgg
     1681 gcgcgagccg gaccgcggtt tggaggtgca tgaccgcgtc gtcccagttc gccttgtcca
     1741 tgaagccgcc gccgaacaac gccttggcga agaagcgctg aaagccggac aaccgcttga
     1801 cctcggcatt ccaggcgccg agcacgtggt gggcattgtc gtaggtggag tcgatttgta
     1861 cagcgcgcgt cgcttcgtcg taaatgattt tggcgtaccg cacccgttcc ttgctgcctt
     1921 tggtgcgtga caggcggccc agcaccatcg agagcgcgta gtgcccgtcg ggccgcgtcg
     1981 aatccgcgcg aatcgcgcgc tcggcatagc cccggcccac ggaataaagg ctatcgcggc
     2041 gccgcttgag ggaatcggca ttcccctgta tttgtttggc gacgtcggcg atcgcgcgcg
     2101 cagccttcca gtttgcagca tagctcagcg agtcgacggc cagggccgcc tggtagtggc
     2161 ggagttggat gtccggatgc atcaggcccg cgctgtcacc gagcgcgaca tgctgggccg
     2221 ccgtctgggc ggcgagagtc gcgccgaaga gcggagcgag cagacacaag gagaggcgca
     2281 tgaaacaatg atcgtcccac ccctgatcga gcgcaagcgc gacggcggca cgctgagtcc
     2341 cgaagagtgg tcagccctcg tcgccgaata cacagccggg cggatccccg actatcagat
     2401 cgcggc
//
LOCUS       DASPZD010000225         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_602627, whole genome shotgun sequence.
ACCESSION   DASPZD010000225 DASPZD010000000
VERSION     DASPZD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602627"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEM33_04360"
     CDS             <1..295
                     /locus_tag="VEM33_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HYS51086.1"
                     /translation="VMVLNISEGQTDYATRVAATLKQAGYRCEADLRNEKITYKIREH
                     SLQKLPYQLIVGEKEKAAEKVAVRTRSGEDLGQMSLPDFVARLRSEAKAGSTA"
     gene            321..917
                     /gene="infC"
                     /locus_tag="VEM33_04365"
     CDS             321..917
                     /gene="infC"
                     /locus_tag="VEM33_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HYS51087.1"
                     /translation="MATERTQRINIEINAPEVRLIGAAGEQVGIVPIAQALKMAEEAE
                     VDLVEIAPLAKPPVCKLMDYGKFKYREAKKAHEAKLKQKQIHVKEVKFRPGTDEGDYK
                     IKLRNLIKFLDEGDKTKVTLRYRGREMAHQEFGVRLIERVKQDLEPHAVVEQYPKMEG
                     RQLIMVLAPKKGKKETKKPVKKEAKKEPKKEPKPEVSP"
     gene            1008..1205
                     /gene="rpmI"
                     /locus_tag="VEM33_04370"
     CDS             1008..1205
                     /gene="rpmI"
                     /locus_tag="VEM33_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HYS51088.1"
                     /translation="MPKLKTKRSAAKRFKIRSGGSIKRSQAFKRHILTKKTTKNKRHL
                     RGTAEVHATDKQHVRAMLPYA"
     gene            1218..1577
                     /gene="rplT"
                     /locus_tag="VEM33_04375"
     CDS             1218..1577
                     /gene="rplT"
                     /locus_tag="VEM33_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HYS51089.1"
                     /translation="MPRVKRGVTARAKHKKVIAQAKGFRGRRGNVFNIAKEAVMKAGQ
                     YAYRDRKQRKRQFRALWIARINAAVREHGMTYSAFMHGLKKASIEVDRKVLADLAVVD
                     KPAFAQLASQAKAKLGA"
     gene            1644..2669
                     /gene="pheS"
                     /locus_tag="VEM33_04380"
     CDS             1644..2669
                     /gene="pheS"
                     /locus_tag="VEM33_04380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYS51090.1"
                     /translation="MDLDKVVSRAIAEFSGTNDANDLEHIKARYLGKSGVLSEARKDL
                     AKLAPDERRDVGARFNQAKDKIEAALAARREALAAAELDSRLAEEALDVTLPGRGLAR
                     GGLHPATRTLERIEQLFRSIGFDVADGPEIETDFHNFTALNQPQDHPARSMHDTFYLK
                     GAGQNLLLRTHTSPIQVRYMQHHEPPFKIIAPGRVYRVDSDATHTPMFHQVEGLWIDE
                     AVSLADLKGVLTDFLRKFFEDEKLRTRFRPSFFPFTEPSAEVDMSCVFCGGAGCRVCG
                     QTGWLEVAGSGQVHPNVLKACGVDHEKYMGFAFGMGPDRLTMLRYGIDDLRLFYDGDL
                     RFLAQFT"
     gene            2666..>4722
                     /gene="pheT"
                     /locus_tag="VEM33_04385"
     CDS             2666..>4722
                     /gene="pheT"
                     /locus_tag="VEM33_04385"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYS51091.1"
                     /translation="MKFPESWLRSFVDPKISTRELARVLTMGGLDVESVEPVAPAFDK
                     VVVGQVLQAAEHPNADRLTLCKVDVGGGTTLAIVCGAPNVAAGMKAPVALLGARLPGM
                     EIKQAKVRGVESSGMLCSARDLGLSQDHSGLLVLPVDAQPGADVRGVLDLDDQLITLK
                     LTPNRGDCLSLRGIAREVSILTATSLIVPAVTPAKAALEEKREILLQDTAACPRYCGR
                     IIRSVDVKARTPDWMVRRLERCGMRSISAIVDITNFVMLELGQPLHAFDNRAIEGEVI
                     VRRARKGERLRLLNGQEIELTPDVLLIADTKKPLALGGVMGGEASAVSGSTAEVFLEA
                     AWFNPSAVAGRARRFALSSDAAFRFERGVDFATTPEAIERATQLVLDVCGGAAGPISE
                     AAAALPQRNPVRLRSSRASKIIGVQFSDEQIAGLLKRLQVPHARSGGEFAVTPPSYRF
                     DLEIEEDMVEEIARLHGYDNIPSRPPRAALAMLAVPEGRRTLSELKRILVDREYFEVV
                     NFSFVEAQWESDFCGNAEPIALENPIAAQLDVMRSSFIGSLVANLRFNLARKVDRVRV
                     FEAARCFLRVSGKEDPRDPARALAGYHQPLRIAGLAYGPAAAEQWGIPVRPVDFYDVK
                     GDVEALLAPREARFAPLAHPGLHPGRAASVILGGAAIGWLGELHPRWLRKYDLPRAPV
                     LFEL"
BASE COUNT          940 a         1452 c         1506 g          824 t
ORIGIN      
        1 ggtgatggtg ctcaatattt cggaggggca gaccgattac gccactcggg tcgcggctac
       61 cctgaagcag gccggctacc gctgcgaagc cgacttgagg aacgagaaaa taacctataa
      121 aattcgggaa catagcttac agaagctgcc ttaccagctg atcgtcggcg aaaaagagaa
      181 ggcagccgaa aaggtggccg tgcgtacccg cagcggtgaa gatctcgggc agatgtcctt
      241 gccggacttc gtcgcccgcc tccgcagcga ggccaaggca gggagtacgg cttagttttt
      301 aattttctgg aggatgcaac atcgccacgg agagaacgca gcgtatcaac atcgaaatca
      361 acgcgcccga agtgcgcttg atcggagcag ccggcgagca agtcggcatc gtgccgatcg
      421 cccaggccct gaaaatggcc gaggaagccg aggtcgatct ggtggaaatc gccccgctcg
      481 ccaagccgcc cgtctgcaag ctgatggact acggcaagtt caagtaccgc gaggcgaaga
      541 aagcccacga agccaagctc aagcagaagc agatccacgt caaagaggtg aagttccgtc
      601 ccggaaccga cgaaggcgac tacaagatca agctgcgcaa cttgatcaag ttcctggatg
      661 aaggcgacaa gaccaaggtt actttgcgct accgcggccg ggagatggcg catcaggaat
      721 ttggcgttcg gctgatcgaa cgggtcaagc aggatctcga gccgcacgcg gtcgtggagc
      781 agtatccgaa gatggaaggg cggcaattga tcatggtgtt ggctccgaag aaaggcaaga
      841 aagaaaccaa gaagccggtg aaaaaggaag ccaagaagga acccaagaaa gaaccgaaac
      901 ctgaagtttc gccctagggc gacggcaggt ttcagaagaa gtggtgtcga ggtcggaaag
      961 cgctccgttg gggccacccg ccgccatgcc aaaacaggag caatgcaatg cccaaattga
     1021 agaccaagcg gagcgcggcc aagcgcttca agatccgctc cggtgggagt atcaagcgct
     1081 cgcaagcgtt caaacgccac atcctcacca agaagaccac caaaaacaaa cgccatttgc
     1141 gcggaactgc ggaggtgcac gccacggaca agcagcatgt ccgcgcgatg ctcccttacg
     1201 cgtaaaggag agccgccatg ccccgagtca aacgcggcgt aaccgcgcgc gccaagcaca
     1261 agaaagtcat cgctcaggcc aagggattcc gcggccgtcg cggcaacgtt ttcaacatcg
     1321 ccaaggaagc ggtgatgaag gcggggcagt atgcctaccg tgaccgcaag cagcgcaagc
     1381 gccagtttcg cgccctctgg atcgcgcgca tcaacgcggc cgttcgcgag cacggcatga
     1441 cgtactcggc tttcatgcac ggtctgaaga aggcctcgat cgaggtcgat cgcaaggtgc
     1501 tcgcggactt ggccgttgtc gacaagccgg ctttcgcgca acttgcgagc caggcgaagg
     1561 ccaagctcgg cgcctaatac ggaagatccc ctccttcccc gggaagggtg gcgcaccgcg
     1621 ccgaggcggt cgaatttcag atgatcgatc tggataaagt tgtttcgagg gcgatcgccg
     1681 agttcagcgg cacgaacgac gcgaacgatc tcgagcacat caaggcacgc tacctcggga
     1741 agtcgggcgt gctgagcgag gcgcggaagg accttgccaa gctcgcgccg gacgagcgcc
     1801 gcgacgtcgg cgcgcgtttc aaccaagcga aagacaagat cgaagctgcg ctcgccgccc
     1861 gccgcgaggc actggccgcc gccgagctcg attcgcggct cgcggaagag gcgctggatg
     1921 tgactctgcc ggggcgcggc cttgcccgcg gcggcttgca tcccgcgacg cgcacgctcg
     1981 agcgcatcga gcagcttttt cgctcgattg gattcgacgt ggcggacggt cccgagatcg
     2041 agaccgactt ccacaacttc actgcgctca atcagccgca ggaccacccc gcgcgttcga
     2101 tgcacgacac gttttacctg aagggcgccg ggcaaaacct gcttctgcgc acgcacacct
     2161 cgccgatcca ggtgcgctac atgcagcatc acgagcctcc gttcaagatc atcgcgcctg
     2221 gccgggtgta ccgcgtagat tcggacgcca cgcacacgcc gatgtttcac caggtggaag
     2281 gcctgtggat cgacgaggcc gtcagcctcg cggatctcaa gggcgttctg accgatttcc
     2341 tgcgcaagtt cttcgaagac gagaagctgc gaacgcgctt tcggccatct tttttccctt
     2401 tcaccgaacc gtcggccgaa gtcgacatga gctgcgtatt ttgcggcgga gcgggctgcc
     2461 gggtctgcgg gcaaaccggt tggctcgagg tagccggcag cggccaagtg caccccaacg
     2521 tactcaaggc ctgcggcgtg gaccacgaga agtacatggg cttcgctttc ggcatgggtc
     2581 ccgatcggtt gactatgctg cgttacggga tcgacgactt gcgacttttc tacgatggcg
     2641 atctgcgttt tctcgcccag ttcacgtgaa atttccagaa agctggctgc gctcgttcgt
     2701 cgatccgaag atatcgacgc gagagctcgc gcgcgtgctt accatgggcg ggctcgacgt
     2761 cgaatcggtc gaacccgtcg cgcccgcttt cgacaaggtc gtagtcgggc aagtccttca
     2821 ggccgccgag catcccaacg ctgaccggct tacgctctgc aaagtcgacg tcggcggtgg
     2881 tacgacgctt gcgatcgtgt gcggagcacc caacgtcgcc gccggaatga aagccccggt
     2941 ggcgctgttg ggggcgcggc tcccgggtat ggaaatcaag caagcgaagg tccgcggcgt
     3001 cgagtcgagc ggcatgctgt gctcggcgag ggacctgggt ctttcgcagg atcattccgg
     3061 cctgcttgtc ttgccggtgg atgcgcagcc tggcgcggat gtgcgcgggg tgctcgacct
     3121 cgatgatcag ctgatcacgc tcaagctcac gcccaaccgc ggggactgcc tgagcttgcg
     3181 gggcatcgcg cgcgaagtct cgatcctcac cgccacgtcg cttatcgtgc ccgccgtgac
     3241 tccggcgaag gccgcactgg aggaaaaacg cgagatcctg ctccaagata cggctgcgtg
     3301 tccgcgctac tgcggccgca tcatacgctc cgttgacgta aaagcacgaa cgcccgactg
     3361 gatggtgcgc cggttggagc gttgcgggat gcgctcgatt tcggctatcg tcgacatcac
     3421 caacttcgtg atgctcgagc tcgggcagcc cctgcacgct ttcgataacc gtgcgatcga
     3481 aggcgaagta atcgtgcggc gcgcccgcaa gggtgagcgc ctcaggctgc tcaacgggca
     3541 ggagatcgaa ttaacccccg acgtgctgct gatcgccgac acaaagaagc cgcttgcgct
     3601 cggtggagtc atgggcgggg aggccagtgc ggtttccggc tcgaccgccg aggtgtttct
     3661 cgaggcggcg tggttcaatc cctccgccgt cgcgggccgt gctcgacgct ttgcgctgtc
     3721 cagcgatgcg gctttccgct tcgagcgcgg cgtggatttt gctacaaccc ctgaggccat
     3781 cgagcgcgcc acgcagctgg ttcttgacgt ctgcggcggc gcggcagggc cgataagcga
     3841 agccgctgcg gcgctgccgc agcgcaatcc ggtgcgactg cgctcgagcc gagcgagcaa
     3901 gatcatcggc gtgcagttct ccgacgagca gatcgcgggc ttgctcaagc gcctgcaggt
     3961 gccgcatgcg cgctcgggcg gagaatttgc ggtaaccccg ccttcgtacc gcttcgatct
     4021 cgagatcgaa gaggacatgg tcgaggaaat cgcacgcctg catggctacg acaacattcc
     4081 atcccgcccg ccgcgggcgg cgctcgccat gctcgccgtt ccggagggcc gcaggaccct
     4141 ctccgagctc aagcggatcc tggtggaccg cgaatacttc gaggtcgtga atttcagttt
     4201 cgttgaggcg cagtgggaga gcgatttctg cggcaatgcc gagccgatcg ccctggaaaa
     4261 tccgattgca gcccagctcg atgtgatgcg ctcgagtttc atcggaagtc tcgtggcgaa
     4321 cctgcgcttc aacctggcgc gcaaggtcga ccgcgtgcgc gttttcgagg ctgctcgctg
     4381 ctttttgcgc gtctccggga aagaggatcc tcgcgatccg gcgcgagcgc tcgccggcta
     4441 ccaccaaccc ctccgcatcg ccggtctagc ctacggcccg gctgctgctg agcaatgggg
     4501 cattcccgtg cgcccggtcg atttctacga cgtcaagggc gatgtggaag cactcctcgc
     4561 tccgcgcgaa gcgcgcttcg cccccctcgc tcatccgggg ctgcacccgg gtcgcgcggc
     4621 gagcgtcatc ttgggtggcg ctgccatcgg ttggctcggc gagttgcatc cgcggtggct
     4681 gcggaaatac gatctgcctc gggcgccggt tctcttcgaa ct
//
LOCUS       DASPZD010000226         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_555300, whole genome shotgun sequence.
ACCESSION   DASPZD010000226 DASPZD010000000
VERSION     DASPZD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555300"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VEM33_04390"
     CDS             complement(<1..1120)
                     /locus_tag="VEM33_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYS51092.1"
                     /translation="MSSPDDFLETPAFDLVARAVQSRLIGTMVSHYRIEERIGSGGMG
                     IVYRARDTRLQRNIALKFLPEAWSADREARAAAALNHPNICTIHEIGEHDGRPFIAME
                     LLEGRTLKQEMGVRNLELGKIIEIASQVADALEAAHGKGIIHRDIKPGNIFITTRGAV
                     KVLDFGIAVMADSPDSHARAAIGTRPYMAPEQLQGRAVDARTDIFSLGVVIQQMAGGT
                     AGFDRILERCLANDPAGRFGSARELRAALQGLRELTFVFADFERRLLERLARRVPRGL
                     RANHFTALGMVGAAGAGVAYALARYNPAWLWVASVMLAVHWLGDSLDGTLARVRGVER
                     PKYGFYLDHVMGALSVVVVGLGIGVSGFVSPALALGVVV"
     gene            complement(1107..1670)
                     /locus_tag="VEM33_04395"
     CDS             complement(1107..1670)
                     /locus_tag="VEM33_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYS51093.1"
                     /translation="MAEEPAKDITRLLHAWSQGDDGALASLMPLVYNELHRSAHFHMA
                     GEAPDNTLQTTALVNEVYLRLLGAREVDWQNRAHFLAVCATLMRRILTDFARARKSQK
                     RGGSAEHVPLDDDILVSNAPRVDLLALDDALTALAAVDERQARVVELRFFGGLDVKET
                     AEVLQVSPETVHRDWRLAKEWLLHELA"
     gene            complement(1963..>2166)
                     /locus_tag="VEM33_04400"
     CDS             complement(1963..>2166)
                     /locus_tag="VEM33_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51094.1"
                     /translation="GDTYFYQHGQFDGSIVYNATPDVQIQAQVLNINNAVFGFYQGTP
                     DHRYNIQREYYRQTFFLGTKIGF"
BASE COUNT          392 a          721 c          669 g          384 t
ORIGIN      
        1 caacgacgac acccaaggcg agcgcgggac tcacgaaacc cgagacccca atccccagcc
       61 ccacaaccac gaccgacagc gcgcccatca catggtcgag gtagaagccg tatttgggcc
      121 gctcgacgcc gcggacgcgc gccagcgtgc cgtcgaggct gtcgccgagc cagtggacgg
      181 ccagcatgac gctcgcgacc cagagccacg cgggattgta ccgggcgagc gcgtacgcga
      241 cccccgcgcc ggccgccccc accatgccga gcgcggtgaa atgattcgcg cgaagaccgc
      301 ggggcacccg gcgcgccagg cgctcgagga ggcgtcgctc gaagtcggcg aacacaaacg
      361 tcagctcacg caagccctgg agtgccgcgc gcaattcccg cgcggaacca aaccgtcccg
      421 ccggatcatt ggccagacac cgttcgagga tgcggtcgaa ccccgccgtt cccccagcca
      481 tttgctgaat gacgacgccc agcgagaaga tatcggtccg cgcatcaacg gctcgcccct
      541 gcagctgctc cggtgccatg tacggccggg tgccaatggc ggcgcgggca tgactgtcgg
      601 gcgagtccgc catcacggcg atcccaaaat cgagcacctt caccgcgccg cgcgtcgtga
      661 tgaagatgtt ccccggcttg atgtcgcgat gaatgatccc cttgccgtgc gcggcctcga
      721 gcgcgtcggc aacctggctc gcgatctcga tgattttccc cagctccaga ttcctgactc
      781 ccatctcctg cttcaacgtc cttccctcga ggagctccat cgcgatgaac gggcggccat
      841 cgtgctcacc gatttcatga atcgtgcaga tgttgggatg attcagggca gccgccgcgc
      901 gcgcttcgcg gtcggcggac catgcttcgg gcagaaactt gagcgcgata ttgcgctgga
      961 gccgggtatc tcgtgcccga tagacgatcc ccatcccgcc gctgccgatc cgctcttcga
     1021 tccggtaatg cgacaccatc gtgccgatca atcgcgattg cacggcacgc gcgaccaggt
     1081 cgaacgcggg agtctcgagg aaatcgtcag gcgagctcat gcagcagcca ttccttggcc
     1141 agtcgccaat cgcgatgcac cgtctcaggc gagacctgca gcacctcggc agtttccttc
     1201 acgtcgagtc cgccaaagaa tcgcagctcc acgacgcggg cttggcgctc gtcgaccgcc
     1261 gcgagggccg tgagcgcgtc gtccagcgcc agcaggtcga cgcgcggagc gttcgaaacg
     1321 aggatgtcgt cgtccagcgg cacgtgctca gccgagccgc cgcgcttctg gcttttgcgc
     1381 gcgcgtgcga agtcggtcag aatccggcgc atcagcgtgg cgcagaccgc gaggaagtgc
     1441 gcgcgattct gccagtccac ttcccgggcg ccgagcaggc gcaagtagac ctcgttgacg
     1501 agcgcggtgg tttgcagggt gttgtcgggc gcttccccgg ccatgtggaa atgcgccgac
     1561 cggtgcagct cgttatacac gaggggcatg agagaagcga gtgcgccgtc gtccccctgc
     1621 gaccacgcgt gcagcaggcg agtgatgtcc ttcgcgggct cctcggccac gctttgaagc
     1681 tatatcaatt ccccatcttt ccgtcgcttt ttctcaaagc gcccggcgac gatgtcatcc
     1741 tgagcggagc gcgccgtagg cgcgcgtagc gaaggacctg ctttttcttt cctttgttac
     1801 gaagcaacgc ggcgtgtcat cctgagcgta gcgcgcgcca gcgcgcgcag cgaaggacct
     1861 gctttttctt ttcttttgtt acgaagcaac gcgccgtgtc atcctgagcg aagcgcgcga
     1921 cagcgcgcgg agcgaaggac ctgctttggg aagcgttgga cattagaaac cgatcttcgt
     1981 accgaggaaa aaggtctgtc gatagtattc ccgctgaatg ttgtaccgat gatccggcgt
     2041 cccctggtag aacccgaaca ccgcgttgtt gatgttgagc acttgtgcct ggatctgcac
     2101 gtcgggcgtc gcgttataca cgatcgagcc gtcgaactgc ccgtgctggt agaaatacgt
     2161 gtcccc
//
LOCUS       DASPZD010000227         3864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_985865, whole genome shotgun sequence.
ACCESSION   DASPZD010000227 DASPZD010000000
VERSION     DASPZD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985865"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /gene="glcE"
                     /locus_tag="VEM33_04405"
     CDS             <1..283
                     /gene="glcE"
                     /locus_tag="VEM33_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HYS51095.1"
                     /translation="ISVPSATPPLRLPGEPLIEWGGALRWLCSRADARTVRDAAKRAG
                     GHATLFRGSDKSVGVFQPLAPALMKIHKELKREFDPQGIFNRGRMYPEL"
     gene            297..1523
                     /gene="glcF"
                     /locus_tag="VEM33_04410"
     CDS             297..1523
                     /gene="glcF"
                     /locus_tag="VEM33_04410"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HYS51096.1"
                     /translation="MQTALADFIRDTPEGREADSILRKCVHCGFCTATCPTYLLLGDE
                     NDGPRGRIYLMKQALEGQEITDRTRLHLDRCLTCRACETTCPSGVQYGRLLDIGRKLV
                     EEKTARPAWERVKRRALAAVLPRRSLFAPLLALGRLARPFLPASLAEKVPAAAPAAGA
                     WPAPRHARKMLALAGCVQPALSPDINAAAARVLDRVGISLIEAKGAGCCGGLRYHLNY
                     QEDGLDDMRALIDSWWPHVENGAEAIVMTASGCGVTVKEYAHLLERDPRYAAKAAKIS
                     ALTRDIGEVIEAEFGRIEPILANKERVRLAFHPPCTLQHGQNIKGLTERLLARAGFEL
                     TPVPDSHLCCGSAGTYSLLQSDLSEKLKRNKLSALGSGDPEVIATANIGCQVHLAAGA
                     ELSVRHWITAFEERLN"
     gene            1524..3338
                     /locus_tag="VEM33_04415"
     CDS             1524..3338
                     /locus_tag="VEM33_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYS51097.1"
                     /translation="MARLIDNIPERPPNRDIRQLARVLRFLWPYRGRMAVAGIALVVA
                     AGCVLVLGQGLKRVVDAGFSGSDATSLDTALFALLAIVCVLALATYARFYSVSWIGER
                     VSADIRRAVFGHLLEQSPGFFETMRTGEAISRLTNDTALLEVVIGSSASFALRNTLIM
                     LGALVMLVITSPKLTALVVLGVPLVIAPVLLFGRRVRKLSRASQDRIADVSAYIDEAL
                     HEIRTVQSYVHEDRDRERFAERVEQAFGTAVRRIGQRALLVALVMLLAFGAVGVILWI
                     GGHDVLAGRLSAGELSAFVFYAVLVAGAVGAVSEVIGDLQRAAGATERLMELLATEPA
                     IRAPANPVALPEPRGNEKTGEVRFERVTFHYPSRPDTAALSDFSLSVSRGEKVALVGP
                     SGAGKSTVFQLLLRYYDPDAGAIALDGVELKKADPKAVRRRIALVPQDPVIFAASVAE
                     NVRYGRPEASDPEVIAACKAAYASEFIERLPEGMNSFLGERGVRLSGGQRQRLSIARA
                     LLADRAVLLLDEATSSLDAESESTVQKALEHLMRGRTTLIVAHRLATVKNADRIVVMD
                     LGRVIAAGTHSELVREEGLYARLAALQFLGEISPAAVE"
     gene            3335..>3864
                     /locus_tag="VEM33_04420"
     CDS             3335..>3864
                     /locus_tag="VEM33_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYS51098.1"
                     /translation="MRVLPLLALLFALPVQADTLRIGSKRFTESYILGEVLRRAVEGK
                     VPVEHRPGLGNTGIVFAALKAGSIDLYPEYTGTIAREILKVDGNPGIRALNAALAPQG
                     LGVAVRLGFNNSYALAMREDRAQALAIRTLSDLARHPGLKLGLSQEFIGRSDGWPGLK
                     AAYKMPYATPSGLDHGL"
BASE COUNT          591 a         1331 c         1304 g          638 t
ORIGIN      
        1 catctcggtt ccgtcggcca cgccgcccct caggcttccc ggagaaccgc tcatcgagtg
       61 gggcggggcg ctgcgctggc tctgctcgcg cgcggacgcg cgcaccgtcc gcgatgcggc
      121 gaagcgcgcc gggggccacg ccacgctgtt tcgcggaagc gacaaatcgg tcggcgtttt
      181 tcagccgctc gccccggcgc tcatgaagat ccacaaggag ctcaagcgcg agttcgatcc
      241 gcagggaatt ttcaatcgcg gccgcatgta tccggagcta tgacggaaaa tcgctgatgc
      301 agaccgctct ggccgatttc atccgcgaca cgcccgaagg gcgcgaagcc gactccatcc
      361 tccgcaagtg cgtgcactgc ggcttctgca ccgcgacctg tccgacctac ctgctcctcg
      421 gggacgagaa cgacggcccg cgaggccgca tctatctcat gaagcaggcg ctcgagggtc
      481 aggagatcac cgacaggacg cggctgcacc tcgaccgctg cctcacctgc cgcgcctgcg
      541 agacgacctg cccgtcgggc gtgcagtacg ggcgcctgct cgacatcggt cgcaagctgg
      601 tcgaggaaaa aacggcgcgg cccgcatggg agcgcgtcaa gcgccgcgcg ctcgcggcgg
      661 tgctgccgcg ccgttccctg ttcgcgccgc tccttgcgct cggccggctc gcgcggccat
      721 tccttcccgc gagcctcgcc gaaaaagtgc cggccgcggc gccggcagcg ggcgcgtggc
      781 ccgcgccgcg ccacgcgcgg aaaatgctcg cgcttgccgg ctgcgtgcag cccgcgctct
      841 cccccgacat caacgcggcg gcggcgcggg tgctcgaccg cgtcggaatt tcgctcatcg
      901 aagccaaggg cgcgggctgc tgcggcgggc tgcgctacca cctgaactat caggaagacg
      961 ggctcgacga catgcgcgcg ctcatcgatt cctggtggcc gcacgtcgag aacggcgcgg
     1021 aagcgatcgt catgactgcg agcggatgcg gcgtcacggt gaaggaatac gctcaccttc
     1081 tcgagcgcga tccccgctac gctgcgaagg ccgcgaaaat cagcgcattg acgcgcgaca
     1141 tcggcgaagt gatcgaagcg gagttcggcc gtatcgagcc gattctcgcc aataaggagc
     1201 gcgttcgcct ggccttccat ccgccctgca ccctgcagca tgggcaaaac ataaaaggcc
     1261 tgaccgagcg cttgctcgcg cgcgcggggt tcgagctgac gccggtgccc gattcgcatt
     1321 tgtgctgcgg ttctgcggga acttattccc tgttgcaatc ggatctgtcc gagaagctca
     1381 agcggaacaa gctttcggcg ctcggctcgg gcgaccccga ggtgatcgcc accgccaata
     1441 tcggctgcca ggtgcatctc gcagccggtg ccgaactgtc ggtaaggcac tggattacgg
     1501 cattcgaaga acggctgaac taagtggcgc ggctgataga caacatcccc gagcgcccgc
     1561 ccaaccgcga tatccggcag ctggcgcggg tgctgcgctt cctctggccg taccggggac
     1621 gcatggccgt cgccggcatc gccttggtgg ttgcggcggg ctgcgtgctc gtcctgggcc
     1681 agggcttgaa gcgcgtcgtc gacgccggct tctcgggcag cgacgcgacg tccctggaca
     1741 cggcgctctt cgcgctgctc gccatcgtct gcgtgctcgc cctggcgact tatgcgcgtt
     1801 tctactccgt gtcctggatc ggcgagcgcg tttccgccga catccgccgg gcggtgttcg
     1861 ggcacctgct ggaacagtct cccgggttct tcgagaccat gcgcaccggc gaagcgatct
     1921 cgcgcctcac caacgacacc gcgctcctgg aagtggtgat cggctcctcg gcttcgtttg
     1981 cgttgcggaa caccctgatc atgctcgggg cgctggtgat gctggtgatc accagtccca
     2041 agctcaccgc gctggtcgtg ctcggcgttc cgctggtgat cgcgccggtc ctgctgttcg
     2101 gccggcgcgt gcgaaaactc tcgcgcgcga gccaggaccg gatcgccgac gtgagcgcct
     2161 atatcgacga agccctgcac gagatccgca ccgtgcagtc ctacgtccac gaggacaggg
     2221 accgcgagcg tttcgccgag cgcgtcgagc aggccttcgg caccgcggtg aggcgaatcg
     2281 gccagcgcgc gctcctcgtc gctctggtga tgctgctcgc cttcggcgcg gtgggcgtca
     2341 tcctctggat cggcggccac gacgtgctcg ccgggcgact gagcgcgggc gagctctccg
     2401 ccttcgtgtt ctacgcggtg ctcgtcgccg gagcggtggg cgcggtgtcc gaagtgatcg
     2461 gcgatctgca gcgcgcggcg ggcgcgaccg agcgcctgat ggagttgctc gcgaccgagc
     2521 cggctatccg cgccccggca aatcccgtag cgctgcccga gccccgagga aacgaaaaaa
     2581 ctggggaggt gcgtttcgag cgcgtgacgt ttcactatcc gtcgcgcccg gacaccgcgg
     2641 cgctctcgga tttctccctt tccgtttcgc gcggcgagaa ggtcgcgctg gtggggcctt
     2701 ccggcgccgg caagtccacg gtgttccagc tgctgctgcg ctactacgat cccgacgccg
     2761 gcgcgatcgc gctggacgga gtcgagctga aaaaagccga cccgaaggcg gtgcgcaggc
     2821 gcattgcgct ggtgccgcag gacccggtga tcttcgccgc gagcgtcgcc gagaacgtgc
     2881 gctacggcag gccggaggcg agcgatcccg aggtcatcgc cgcctgcaag gcggcctacg
     2941 cgagcgagtt tatcgagcgt cttcccgagg ggatgaattc attcttgggc gagcgcggcg
     3001 tgcgcctttc gggcggccag cgccagcgcc tgtcgatcgc ccgcgcgctg ctcgccgacc
     3061 gcgcggtgct gctcctggac gaggcgacga gctcgctcga cgccgagagc gaaagcactg
     3121 tgcagaaggc gctcgagcac ctgatgaggg gccgcaccac gctcatcgtc gcgcaccgcc
     3181 tcgccaccgt gaagaacgcc gaccgcatcg tggtcatgga tctgggccgg gtgatcgccg
     3241 cgggcacgca ctcggagctg gtgcgcgaag aaggcctcta cgcgaggctc gctgcgctgc
     3301 agtttctcgg cgaaatctcg ccggcggcgg tggaatgagg gtcctcccgc tcctcgcgct
     3361 gctcttcgcg ctgccggtgc aggccgacac gctgcgcatc ggctccaagc gttttaccga
     3421 gtcctacatc ttgggcgagg tgctgcgccg ggcggtcgaa ggcaaggttc ccgtcgaaca
     3481 ccggcccggc ctcggcaaca ccggcattgt gttcgcggcg ctcaaggcag gctcgatcga
     3541 cctctacccc gagtacacgg ggacgatcgc gagggagatc ctcaaggtcg acggcaatcc
     3601 gggcataagg gctctcaacg ccgccctcgc gcctcagggc ctcggcgtcg cggtgcggct
     3661 cggcttcaac aacagctacg cgctcgcgat gcgcgaggac cgcgcacagg ctctggcgat
     3721 ccgcacgctt tcggacctcg cgaggcaccc gggcctgaag ctcggcctgt cgcaggaatt
     3781 catcggccgc agcgacggct ggccggggtt gaaagcggca tacaagatgc cctacgccac
     3841 cccttcggga ctcgatcacg gcct
//
LOCUS       DASPZD010000228         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_13952, whole genome shotgun sequence.
ACCESSION   DASPZD010000228 DASPZD010000000
VERSION     DASPZD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13952"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VEM33_04425"
     CDS             <1..679
                     /locus_tag="VEM33_04425"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYS51099.1"
                     /translation="MVPKSEYQIIDTWYAMGMVGTGSKDIEVKEIFVPERRALALARC
                     RGGLEHPGAKLNNGPLFRVPIVASAGHPLSATALGAAEGAFQHVVNSFKTRLGTYTGA
                     KVSDFQAVQIKIAEARCLIDSATALMRGSAVAFQELARRNQVPDLETKLRFRAQNALA
                     VRQAREAVEVLWSCYGANAIYTRDPLQRFLRDLQALSQHFSFSFDVAGAAYGTAVMGG
                     KYVNPTM"
     gene            676..1497
                     /locus_tag="VEM33_04430"
     CDS             676..1497
                     /locus_tag="VEM33_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HYS51100.1"
                     /translation="MKIGELLVCQISDLHIKAPGRLSYRVVDCAAMLARCVAEILRLP
                     QRPDLVLATGDLTDSGRAEEYAHLRELLAPLPMPVYFIPGNHDERGALRAAFPEHAYL
                     RQWDAFVQYAIDDWPLRIVAIDTVIPGEDGGRLDDERLAWLEKILSLRRDKPTLLIMH
                     HPPFPTLIGHMDQIGLEGSERLAELIERHPQVERVLCGHLHRPIQFRFAGTIASTCPS
                     PAHQVALDLAPDAASAFRMEPPAFQLHAWREGVGVVSHTAYIGDFAGPYPFYEGD"
     gene            1552..2055
                     /locus_tag="VEM33_04435"
     CDS             1552..2055
                     /locus_tag="VEM33_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51101.1"
                     /translation="MPVTGLNHYLIVAKNLERTRDFYCKVLGMELAERPDFGFPGYWL
                     KVGDEICVHLASQDPNRIRDMFLLKKHPKGTTGSGSVDHIAFLAQNPGAVRERIQKNK
                     VAMHFRSFPDAKLFQIFLKDPDDITIELNFLGEVIDEKEWQGQGSASAMTLTKAKGKR
                     TFLARQN"
BASE COUNT          406 a          764 c          672 g          362 t
ORIGIN      
        1 catggtgccg aagagcgagt accagatcat cgacacgtgg tacgcgatgg gcatggtcgg
       61 caccggcagc aaggacatcg aggtgaagga gatcttcgtt ccggagcggc gcgcgctcgc
      121 gctcgcgcgc tgccgcggcg gcctcgagca cccgggggcg aagctcaaca acgggccgct
      181 gttcagagtg cctatcgtcg cctccgccgg gcatccgctg tccgccaccg cattaggcgc
      241 ggcggaaggc gctttccagc acgtggtcaa ctcgttcaag acccgcctgg gcacttacac
      301 cggcgcgaaa gtctccgatt tccaggcggt gcagatcaag atcgccgagg cgcgctgtct
      361 catcgattcg gccaccgccc tgatgcgcgg ctccgccgtg gcgttccagg agcttgcgcg
      421 aaggaaccag gtgccggacc tggagaccaa gctgcgcttc cgcgcgcaga acgctctcgc
      481 cgtgcgccag gcgcgcgaag cggtggaagt tctgtggtcc tgctacggcg ccaacgccat
      541 ctacacccgc gaccccctgc agcgcttcct gcgcgacctg caggcgctga gccagcactt
      601 ctcgttcagc ttcgacgttg ccggtgcagc atatggaacg gcggtcatgg gcgggaaata
      661 cgtgaaccca acaatgtgaa gatcggggag cttctcgtct gccagatcag cgacctgcac
      721 atcaaggcgc cgggcaggct ttcctatcgc gtggtggact gcgccgcgat gctcgcgcgc
      781 tgcgtcgcgg aaatcctgcg cctgccgcag cgccccgacc tcgtgctcgc caccggcgat
      841 ctcaccgatt ccggccgggc ggaggaatac gctcatctac gcgagctgct cgccccgctt
      901 cccatgccgg tgtatttcat tcccggcaac cacgacgagc gcggcgcgtt gcgcgcagct
      961 tttccggagc acgcctacct gcgccagtgg gatgccttcg tgcagtacgc gatcgatgac
     1021 tggccgctgc gcatcgtcgc gatcgacacc gtcattccag gcgaggacgg cgggcggctg
     1081 gacgacgagc ggctcgcctg gctggagaag atcctctccc tgaggcgcga caagccaacg
     1141 cttctgatca tgcaccaccc gcccttcccc acgctgatcg gccacatgga ccagatcggc
     1201 ctggaaggaa gcgagcgcct ggccgagctc atcgagcggc acccgcaggt ggagcgcgtg
     1261 ctttgcggcc atctgcaccg gccgatccag ttccgcttcg ccgggacgat cgcgtcgacc
     1321 tgcccgagcc cggcgcacca ggtagcgctc gacctcgccc ccgacgccgc gtcggccttc
     1381 aggatggagc cgccggcgtt ccagctgcac gcctggagag agggcgtcgg cgtcgtcagc
     1441 cataccgcct acatcggcga tttcgccgga ccctatccct tctacgaggg cgactaaccg
     1501 atcgagcaga agggtggaaa atacgccaac ccgacctgta aaggaggagc catgcccgtt
     1561 accggactga accactacct catcgtcgcg aagaacctcg agcgcaccag ggacttctac
     1621 tgcaaagtcc tgggcatgga gctcgccgag cgcccggact tcggctttcc cggctactgg
     1681 ctcaaggtcg gcgacgagat ctgcgtccac ctcgcctccc aggatcccaa caggatccgc
     1741 gacatgttcc tgctgaagaa gcacccgaag gggaccaccg gctccggctc ggtcgatcac
     1801 atcgccttcc tcgcccagaa cccgggcgcg gtgcgcgagc gcatccagaa gaacaaggtc
     1861 gcgatgcact tccgcagctt ccccgacgcg aagctcttcc agatcttcct caaggacccg
     1921 gacgacatca ccatcgagct caacttcctc ggcgaagtga tcgacgagaa ggaatggcag
     1981 ggccagggct cggcctcggc gatgaccctg accaaggcca agggaaagcg cactttcctc
     2041 gcgcgacaga attgaaatca gggacagacc acgttttccc ctgccctccg gcgcgagcgt
     2101 cctgtgccgt cactgttacg aacgggccga tttgcctttg caattattgc aagcctcccg
     2161 ccacgatcaa atgggaataa gaacctggcc acgacccgct ttag
//
LOCUS       DASPZD010000229         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_79644, whole genome shotgun sequence.
ACCESSION   DASPZD010000229 DASPZD010000000
VERSION     DASPZD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79644"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VEM33_04440"
     CDS             complement(<1..591)
                     /locus_tag="VEM33_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYS51102.1"
                     /translation="MVTLKEIADHFTSILVVVLVVLVVATAIAVVRGAIDQIKEITAE
                     DPFLFLRLFTRGGPLPLVALLSFLVPLLAIGLGFDSVNGEHNRRTLSRILAQPIYRDA
                     LLFGKFAAGLFTLAVSLIVLWLLVIGLGLLGLGVPPNAEEMARALVLLVVTIVYAGFW
                     FALALLCSVVFRSAATAALVALGLWLFLTILWPLFSP"
     gene            complement(617..1564)
                     /locus_tag="VEM33_04445"
     CDS             complement(617..1564)
                     /locus_tag="VEM33_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51103.1"
                     /translation="MSANVIEAKNLTKRYNGVAVVKGISFSVARGEIFGLLGPNGAGK
                     TTTILMLLGLSDISDGQARVLDHDPVREPLAVKRQVGYLPDQIGFYDNLTAAENLRYT
                     ARLMGLERAEREQRIKSSLAHVGLADVANNRVATFSRGMRQRLGLAEILMKEAQIAIL
                     DEPTSGLDPQATAELLNIIRDLKNQGVSVLLSSHLLERVQSVCDRVALFSQGNIALIG
                     TVPELGRQVLGGGFRVEVEAEGQDLVQRIAAIPGVQRVEPAGANRFLLLADHDVRPEA
                     AAAVVAAGGRLLRLSVEEPSLEAIYTRYFQTHEGELHAA"
     gene            complement(1561..>2373)
                     /locus_tag="VEM33_04450"
     CDS             complement(1561..>2373)
                     /locus_tag="VEM33_04450"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HYS51104.1"
                     /translation="VAARGATSNATLPIEVRLATDLPAKLTLNPQLPELRGTSKSSFE
                     FQLALKNDSPKKVLVSLSAQAPQNFDATYTEQYGSQELNALPLDPGQSKDVKLKVRPP
                     NTIAAGKYKVTAKVSADDATATSDLVLDIAGQPKIDIAGREGVLSAKASAGQETTIPV
                     ILTNSGTAPAEQIELSGTAPTGWKVSFEPKTVERIAPNENKEVQALVTPAAKAVAGDY
                     VTSVRASARGESASQSFRVAVTTSTIWGIVGIALIGGALLVMIGAVVWFGRR"
BASE COUNT          434 a          776 c          761 g          402 t
ORIGIN      
        1 cggcgagaat agcggccaca ggatagtgag gaacagccac agcccgagcg cgaccagggc
       61 ggccgttgcc gccgaccgaa acaccaccga gcacagcagc gcgagcgcga accagaagcc
      121 ggcataaacg atcgtcacca ccaataggac gagcgcgcgc gccatctcct cggcattggg
      181 aggaactccg agcccgagca ggccgagccc gatcaccagc agccagagca cgatgaggct
      241 caccgccaag gtgaacagcc cggcggcgaa tttcccgaac aacagcgcgt cgcgatagat
      301 cggctgcgcc aggatgcgcg agagcgtgcg ccggttgtgc tcgccgttca cggagtcgaa
      361 gcccaggccg atcgcaagga gcggcaccag gaacgacagc agcgccacca gcggcaatgg
      421 cccgccgcgg gtgaacaacc gcaggaacag gaacggatct tccgccgtga tttccttgat
      481 ctgatcgatg gcgccgcgca ccaccgcgat cgcggtcgcc accaccagca cgaccagcac
      541 cacgacgagg atgctggtga aatggtcggc gatctccttc agcgtcacca ccccgaagcc
      601 gtgcagagcc gaaccttcac gccgcatgca gctctccttc gtgggtctgg aaatagcgcg
      661 tgtagatcgc ctcgaggctc ggctcctcga ccgataagcg cagcagccgc ccgccggctg
      721 cgacgacggc cgccgcggct tccggccgaa cgtcgtgatc ggccaggagt aggaaccggt
      781 tcgctcctgc cggctcgacc cgttgtaccc cggggattgc ggcgatgcgt tgcacaagat
      841 cctggccttc ggcttcgacc tcgacgcgga agccgccgcc caagacctga cgtcccagct
      901 ccggtaccgt accgatgagc gcgatgttgc cctgactgaa cagggcgacg cgatcgcaaa
      961 cgctctgcac ccgctcgagc aggtgcgagg acaacagaac gctgacgccc tggttcttga
     1021 gatcacggat gatattgagc aactcggcgg tcgcctgcgg atcgagccct gaggttggct
     1081 cgtcgagtat cgcgatctgc gcctctttca tgaggatttc ggcgaggccg agccgctggc
     1141 gcattccgcg cgagaaggtg gcgacccggt tgttcgcaac gtctgcgaga cccacatgcg
     1201 caagtgaaga tttgattcgc tgctcgcgct cggcgcgctc caaccccatc agccgcgccg
     1261 tataacgcaa gttctccgcg gcggtgagat tgtcgtagaa gccgatctgg tcgggcaggt
     1321 aaccgacctg gcgtttcacc gccagcggct cacgcaccgg atcgtggtcg agcacacgcg
     1381 cctgcccgtc ggaaatatcg ctcagcccca gcagcattag aatagtcgtg gtcttgccgg
     1441 caccattcgg accgagcagc ccgaagatct ccccccgcgc cacggaaaac gaaataccct
     1501 tgacgacggc tacgccgttg tagcgcttgg tgagattttt cgcctcgatg acgttggcgc
     1561 tcatcggcgt ccgaaccata ccaccgcgcc gatcatgacc aggagcgctc cgccgatgag
     1621 cgcgatcccc acgatgcccc atatcgtgga ggtcgtgacc gcaacgcgga aggactggga
     1681 tgccgactcc ccgcgcgcgc ttgcgcgcac gctggtgacg tagtcgccgg ccacggcctt
     1741 ggcagccggc gtgacgagcg cctgcacctc cttgttttca ttcggggcga tgcgctcgac
     1801 cgtcttcggc tcgaagctga ctttccagcc cgtcggcgcg gtgcccgaga gctcgatctg
     1861 ctcggccggt gcggtgccgg agttggtgag gatcaccggg atcgttgtct cctggccggc
     1921 ggaagccttc gcgctgagaa cgccttcgcg accggcgata tcgatcttcg gctgaccggc
     1981 gatgtcgaga acgagatcgg aagttgcggt ggcgtcatcc gccgatactt tggccgtcac
     2041 cttgtatttc ccggctgcga tggtgttcgg cgggcgcacc ttgagcttga cgtccttcga
     2101 ctggccggga tcgagcggaa gcgcattaag ctcctggctg ccatattgct cggtataggt
     2161 ggcgtcgaag ttctgcggcg cctgcgcgga caggctcacg agcactttct tgggactgtc
     2221 attcttgagg gcgagctgga actcgaagct cgactttgac gtgccgcgca gctcgggaag
     2281 ttgcgggttg agcgtgagct tggcgggcaa atcggtcgcg agcctcacct cgatgggaag
     2341 cgttgcgttg ctggtcgcgc cgcgcgcggc cac
//
LOCUS       DASPZD010000230         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_511383, whole genome shotgun sequence.
ACCESSION   DASPZD010000230 DASPZD010000000
VERSION     DASPZD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511383"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VEM33_04455"
     CDS             <1..1371
                     /locus_tag="VEM33_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51105.1"
                     /translation="TLLGALPLLIKPDAKTFANIGWGSGLSVEVALSHSGPVLVDTVE
                     IEPAMVAGAHSFSPRVDRAYRDPRSKVYFEDAKSYFARHGKRYDVIMSEPSNVWVSGV
                     AGLFTTEFYRDTKRYLAPGGLFVQWVHAYDLNDRLLGSVVSALGENFADYEIYETNPG
                     DLMVVAVAEGRVPRPKPLPESEGAFMQQLARIGITRSEEIAVRSLGAKKQIGPLFAAL
                     GAPVNSDFHPLVQLEAPRSRFIGSMAGAVQELAAAPLPILEMTGGTQGVYLKQPVPDY
                     VSSLPSRVQSGALEIARVLADRSADPLRASEPAAIPILLALKRPAALCADDPPKTALE
                     LLHRAGEVTLANLAADKRRALWVEHKWLDCTPRSSRVRERFELYGAIAARDAHAMLAR
                     ARALLAGPAQGGDEWGRYLLGTAMLGAYAAGEHEEGRRLWKTYRSTFYPRGEIPPHMV
                     YLANLQ"
     gene            complement(1387..>2322)
                     /locus_tag="VEM33_04460"
     CDS             complement(1387..>2322)
                     /locus_tag="VEM33_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYS51106.1"
                     /translation="HYVGDVGIGINPKLAERVRTADVLLVIGARLGEMTTGAYTLVEP
                     PRPKQKLVHVHAGAEELGRVYQGELLINSGMPQIAAALKGMKLDGSAWRAWREQARAD
                     YLGWTKPTANPGRVQLAEIVAWLRERLPEDAIIANGAGNFAGWISRFYRYRGLRTQVA
                     PTNGSMGYGVPAGVAAKVAHPERVVVSINGDGDFLMNGQELATAVQYGLPVIFIVVNN
                     GMYGTIRMHQERDYPARVYGTALQNPDFAALARAYGAYGAIVEETAQFAPAFERARHE
                     TASSGKPALIEVRIDPQAITTSTTLDAIRARALKG"
BASE COUNT          351 a          803 c          770 g          398 t
ORIGIN      
        1 accctgctcg gcgcgctgcc cttgctcatc aagccggatg cgaagacttt cgccaacatc
       61 ggctggggtt ccggcctgag cgtcgaggtc gcgctctcgc acagcggccc cgtgttggtc
      121 gataccgtcg agatcgagcc ggccatggtg gcaggagccc attccttctc tcctcgggtc
      181 gaccgcgcct accgcgaccc gcggagcaag gtgtatttcg aggacgcgaa gagctatttc
      241 gcccgccacg ggaaacgcta cgacgtgatc atgtccgagc cttccaatgt ctgggtcagc
      301 ggcgtcgcgg ggctcttcac caccgagttc tatcgggata ccaagcgcta cctcgcgccc
      361 gggggcctgt tcgtgcaatg ggtgcacgcc tacgatctca acgaccggtt gctcggctcc
      421 gtcgtttcgg cgctcggcga gaatttcgcg gactatgaaa tctacgaaac gaatcccggc
      481 gacctcatgg tcgtcgcggt cgcggaaggc cgggtgcccc gcccgaagcc gcttcccgaa
      541 tccgaagggg cattcatgca gcaactggcc cggataggca tcacgcgctc cgaggagatc
      601 gcagtgcgca gtctcggcgc caagaagcag atcggaccgc tattcgccgc gctcggcgcg
      661 ccggtcaatt cggactttca ccccttggta cagctcgaag ccccgcggag ccgtttcatc
      721 ggaagcatgg cgggggcggt tcaggaactc gccgctgcgc cgctgccgat tctcgagatg
      781 accggcggca cgcaaggggt gtacctcaag cagccggtcc ccgattacgt gtcctcgctc
      841 ccctcgcggg tccagtcggg tgccctggag attgctcggg tgctcgcgga ccgctcggca
      901 gatcccctgc gcgcgagcga gccggcggcg atccccatcc tgctcgcctt gaagcgcccc
      961 gccgcgctct gcgcggacga cccgccgaag acggcgctcg agctgctgca ccgcgccggc
     1021 gaggtcaccc ttgccaacct cgcggcggac aagcggcgcg ctctctgggt cgagcacaag
     1081 tggctcgact gcacgccccg ttcgtcccgc gtccgcgaaa gattcgagct gtacggcgcg
     1141 atcgcggcgc gcgacgcgca tgcgatgctc gcgcgcgcgc gtgcgctgct tgcgggaccg
     1201 gcccaaggcg gggacgaatg gggccgctac ctgctcggca cggcaatgct cggcgcgtac
     1261 gccgccggcg agcatgagga aggtcgccgg ctttggaaaa cttaccggag cacgttctat
     1321 ccgcgcggcg agattccccc gcacatggtg tacctcgcca acctgcagtg agcctggtgg
     1381 ccggcctcag cctttgagag cgcgtgcgcg aatggcgtcg agcgtggtgg aggtggtgat
     1441 cgcctgcggg tcgatgcgga cttcgatcag cgccggtttg cccgaagacg cggtttcgtg
     1501 ccttgcgcgt tcgaaggccg gtgcgaactg cgccgtctcc tcgacgatcg cgccgtacgc
     1561 gccgtaggcc cgcgcaaggg ccgcgaaatc cgggttctgc agcgcagtgc catagacgcg
     1621 cgccgggtag tcccgctcct gatgcatgcg tatcgtcccg tacatcccgt tgttgacgac
     1681 gatgaatatc acgggcagcc cgtactgcac cgcggtggcg agctcctgcc cgttcataag
     1741 gaagtcgccg tcgccgttta tcgagacgac gacacgctcc ggatgcgcga ctttcgccgc
     1801 gacgcctgcc ggaacgccgt agcccatcga gccgttggtg ggcgcgacct gcgtgcgcag
     1861 gccccgatag cggtagaagc gggagatcca gcccgcgaaa tttcccgcgc cgttcgcgat
     1921 gatggcgtcc tcggggaggc gctcgcgcag ccaggcgacg atctcggcga gctggactct
     1981 gcccgggttc gcggtgggtt tcgtccaccc caggtagtcg gcgcgcgcct gctcgcgcca
     2041 ggcgcgccac gccgaaccgt cgagcttcat tcctttcagg gcggccgcga tctgcggcat
     2101 gcccgaattg atcaacagct cgccctgata gacccggccc agctcctcgg cgcccgcgtg
     2161 cacgtgcacg agcttctgct tcggccgcgg cggctcgacc agcgtgtagg cgccggtggt
     2221 catctcgccc agcctcgcgc cgatcacgag caggacatcg gcggtgcgca cgcgctcggc
     2281 cagcttcggg ttgatgccga ttccgacgtc gcccacgtaa tg
//
LOCUS       DASPZD010000231         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_922485, whole genome shotgun sequence.
ACCESSION   DASPZD010000231 DASPZD010000000
VERSION     DASPZD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922485"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1055)
                     /locus_tag="VEM33_04465"
     CDS             complement(87..1055)
                     /locus_tag="VEM33_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HYS51107.1"
                     /translation="MHIRTLVAVALALAACQGWAQTKWEMPTPYPDGNFHTKNIRQFA
                     DEAGRATRGSLQIQVHSNGALIKHPEIKRAVQTGQVPIGELLISVLANESPLFAFDSN
                     PFVANSYPKETKLWRIARPYLEKALDAQGVKLLYSVPWPPQGIYAKKEIKSTADLKGA
                     KFRTYSPTTARLAELMGAIPTTVQVPDIPQAFKTGLVDSMITSGATGVDTQAWDYLSH
                     YYDTQAFLPQNMVVVNKSAWSKLNSGEQQALLDAARAAETRGWKTSEAENEGYVKTMA
                     SHGIKILKPSAKLTAEFETIGKQMADEWAKKAGAEGEAILRAFQAK"
     gene            1174..>2242
                     /locus_tag="VEM33_04470"
     CDS             1174..>2242
                     /locus_tag="VEM33_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HYS51108.1"
                     /translation="MEKWQFWIDRGGTFTDIVALRPDGRILSHKLLSENPGRYRDAAI
                     AGIRELLAVPSGAAIPGEKIEVVKMGTTVATNALLERKGERTVLFITRGFRDALRIAY
                     QNRPRIFDRHIVLPELLYAKVVEVEERIGARGEVLVPLEAEQTRRDLESAYREGYRSA
                     AIVLMHGYRYKQHERRIAELARAIGYSQVSASHEVSPLMKLVSRGDTTVVDAYLSPIL
                     RRYVDGVARELAGVRLMFMQSNGGLTDARRFAGKDSILSGPAGGVVGAVRASLAAGFD
                     KIIGFDMGGTSTDVSHFAGAQLSDLERAFETQVAGVRMRAPMMSIHTVAAGGGSILHF
                     DGSRYRVGPDSAGANPGPASYR"
BASE COUNT          363 a          730 c          724 g          425 t
ORIGIN      
        1 ggccgcgatc gccaccaaaa aaaatccggc caggaagccg gatcccttgt acagcaggtc
       61 gagcgctccg cgcatccggg gtgggactat ttggcctgga acgccctgag gatcgcctcg
      121 ccctccgcgc cggccttctt cgcccactca tcggccattt gcttgccgat cgtctcgaat
      181 tcggcggtga gcttcgcaga gggcttcaga atcttgattc cgtgcgaagc catggtcttt
      241 acgtagcctt cgttctccgc ctcgctcgtc ttccagcccc gcgtctcggc cgcccgggcg
      301 gcatcgagga gggcctgctg ctcgccggag ttcaacttgc tccatgcgct tttgttcacc
      361 accaccatgt tctgcggaag gaacgcctgt gtgtcgtagt agtgcgaaag ataatcccac
      421 gcctgggtgt ccacgccggt cgcgcccgag gtgatcatcg agtcaaccag accggtcttg
      481 aacgcctgtg gaatatcggg gacttgcacc gtggtcggga tcgcccccat cagttcggcc
      541 agccgcgcgg tggtcgggct gtaggtacgg aattttgcgc ccttcaggtc ggccgtcgat
      601 ttgatctcct ttttcgcgta gatgccctgc ggcggccagg gcaccgaata gagcagcttg
      661 acgccctgcg catcgagtgc cttctcgagg tagggccgcg cgatgcgcca cagcttcgtt
      721 tccttgggat agctgttggc gacaaagggg ttcgaatcga acgcgaacaa aggcgactca
      781 ttggcgagca ccgagatcag caattcgccg atcggcactt gtccggtctg cactgcgcgc
      841 ttgatctcgg gatgcttgat cagcgcgccg ttcgaatgca cctggatctg caggcttccg
      901 cgcgtcgctc tgcctgcttc atcggcgaac tgtcggatgt tttttgtgtg aaagttgccg
      961 tcggggtagg gggtgggcat ctcccatttg gtctgcgccc atccctggca ggcggcgagc
     1021 gccagcgcga cggccacaag tgtcctgata tgcatgattt ctccctccga aggccgagca
     1081 gcgaagtttg tcagacctcc ggcccgtgcg tcaatcgcgg acaaggccgg accaaggccc
     1141 gaacgcgcag cccgcttgtg cgatactttc gacgtggaaa aatggcagtt ctggatcgac
     1201 cgcggcggaa cgtttaccga tatcgtcgcc cttcgcccgg acgggcgcat cctctcccac
     1261 aagctgctct ccgagaatcc cggccgctac cgcgatgccg cgatcgccgg aatacgcgag
     1321 ctgctcgcag tcccctccgg cgcggccatc cccggcgaaa agatcgaggt cgtgaagatg
     1381 ggcacgaccg tggccaccaa cgccctcctc gagcgcaagg gcgagcgcac ggtgctcttc
     1441 atcacgcgcg gttttcgcga cgcgctgcgc atcgcctacc agaaccggcc gcgcatcttc
     1501 gaccgccaca tcgtcctgcc agagctcctg tacgcgaaag tcgtcgaggt cgaggagcgc
     1561 atcggcgcca gaggggaggt gctggttccg ctcgaggccg agcagacccg ccgcgacctc
     1621 gaatcggcat accgcgaggg ctatcgctcc gccgcgatcg tgctgatgca cggttaccgc
     1681 tataagcagc acgagcggag aatagcggag ctggcgcgcg ccatcggcta ctcgcaggtt
     1741 tcggcgtcgc acgaggtgag cccgctgatg aagctcgtgt cgcggggtga caccacggtg
     1801 gtggacgcct acctgtctcc catcctgcga aggtatgtcg acggggttgc aagggagctc
     1861 gccggcgtgc gcctcatgtt catgcagtcg aacgggggct tgaccgatgc gcgccgcttt
     1921 gccggcaagg acagcatcct ctcgggaccc gccggcgggg tggtgggcgc ggtccgtgcc
     1981 tcgctcgccg cgggcttcga caagatcatc ggcttcgaca tgggcggcac ctcgaccgac
     2041 gtgtcgcatt tcgcgggcgc ccagttgagc gatttggagc gcgcgttcga gacccaggtc
     2101 gcgggcgtgc gcatgcgggc gccgatgatg agcatacaca ccgtggcggc gggcgggggc
     2161 tcgatcctgc atttcgacgg ctcacgctac cgcgtcggcc cggactcggc gggcgccaac
     2221 ccgggcccgg cgagctaccg cg
//
LOCUS       DASPZD010000232         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_971461, whole genome shotgun sequence.
ACCESSION   DASPZD010000232 DASPZD010000000
VERSION     DASPZD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971461"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VEM33_04475"
     CDS             <1..544
                     /locus_tag="VEM33_04475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYS51109.1"
                     /translation="LSSIPPKDLSMAEFNRQLDQLSRQMENRSDSLGILESQLFDAKV
                     KKKLMPTIPPVDAAYSASSYGWRIDPFNGMLAMHEGVDFPVDIGTPVFAAAGGVVIYS
                     GPHPQYGFLVEIDHGNDFTTRYAHCSRVLVREGEVVQRGSKIAESGSTGRATGPHLHF
                     EVRYRSVAQNPIRFLQATAR"
     gene            679..3450
                     /gene="secA"
                     /locus_tag="VEM33_04480"
     CDS             679..3450
                     /gene="secA"
                     /locus_tag="VEM33_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HYS51110.1"
                     /translation="MISRALKAVFGSRNDRLLKHYGRSVRVINALEPSMAALPDHGLG
                     AKTLELRERFAKRLQGVPEEERDAAEKAALLELLPESFAVVREAAKRVLNMRHFDMQL
                     IGGMVLHDGKISEMRTGEGKTLVATLPAYLNALSGRGVHIVTVNDYLASRDAEWMGKV
                     YKFLGLSVGVILTQMPHAEKQSAYAADITYGTNNEFGFDYLRDNMAMQVGDRFQRKLN
                     YAIVDEVDSILIDEARTPLIISGRAEDRTEIYQRMDKVAPMLKRQEKESAPGDFYVDE
                     KNHTVLLSEEGHEHAEALLGRAGLLPEGRSLYEPAYVNLMHHLNAALRAHNLFFRDRH
                     YVVQDGEVIIVDEFTGRLMPGRRWSDGLHQAIEAKENVSIQSENQTLASITFQNYFRM
                     YRKLAGMTGTADTEAYEFQEIYGLEVVVVPTHMPMIRADRNDQVFRTAAEKYKAIIGD
                     IKDCHERGQPVLVGTTSIENSELISGMLEKDKLPHQVLNAKQHAREAEIVAQAGRPKM
                     ITIATNMAGRGTDIVLGGNVEKQIDLVRDKDELDAAGKERRISELRHEWQDLHNRVIA
                     AGGLHIIGTERHEARRIDNQLRGRSGRQGDPGSSRFYLSLEDPLLRIFAGERVNAIMQ
                     RLGMPEGEPIEHSLVTRSIESAQRKVEAHNFDIRKQLLEYDNVANDQRREIYHLRNSI
                     LESQDVSDQIAALREGVVTDFFRAHVPEESMEEQWDVPGLEKVYAAELRLELPLSKWL
                     ESDPGLDDAALLKRTIDEAHAAYARKMAANPPEAVHHVERLILLSTLDNHWLEHLAAL
                     DHLRQGIHLRGYAQKNPKQEYKREAFELFGNLVESVKLEVTRTLMTVELQMREQVEQV
                     EKLQAPALENVQYQHAGYEEALAGAGADAAPASKPAPFVRQMGKIGRNDPCPCGSGKK
                     YKHCHGKLS"
     gene            3473..4699
                     /gene="argJ"
                     /locus_tag="VEM33_04485"
     CDS             3473..4699
                     /gene="argJ"
                     /locus_tag="VEM33_04485"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HYS51111.1"
                     /translation="MAVNLSPPRPEDLLLVKGVKLGVAEAGIRKARRKDLLLMTLAEG
                     TSASGVFTQNRFCAAPVQVAKEHLATGSPRALLVNTGNANAGTGEAGIAGARASCDAA
                     ARLVGCESRRVLPFSTGVIMEPLPVERIVAGLPACVADLREDNWARAAEAIMTTDSVP
                     KARSRRVQLAGGEVTVTGIAKGAGMIRPDLATLLAFVATDAPVAQGALRQLLADAAAR
                     SFNRISVDGDTSTNDSFVLIATGASGGTMIDDPGHPGFAPLREAVLEVSQGLAQAIVR
                     DGEGATKFITVRVEKGGDEAECASVAYAIAHSPLVKTAFFASDPNLGRLLAAVGNAGV
                     RDLEVGRVELYLDGVLVSNDGGRAPSYREEDGKRVMAKSEITVRVVLNRGLAATTVWT
                     CDLSRDYVTINAEYRT"
BASE COUNT          940 a         1516 c         1560 g          760 t
ORIGIN      
        1 gctgtcgagc atccctccca aggatctctc gatggcggag ttcaaccggc agctcgatca
       61 gctttccaga cagatggaaa accggagcga ctccctcggg atcctcgagt cgcagctgtt
      121 cgacgccaag gtgaagaaga agctcatgcc gacgatcccc ccggtggacg cggcctacag
      181 cgcatcgagc tacggctggc gcatcgatcc gttcaacgga atgctcgcca tgcacgaggg
      241 cgtggatttc cccgtggata tcgggacccc cgttttcgcg gcggcgggcg gcgtggtgat
      301 ctactcgggg ccgcatccgc aatacggctt cctggtggag atcgaccacg gcaacgattt
      361 tacgacccgt tacgctcatt gctccagagt tctggtcagg gaaggcgagg tagttcagcg
      421 cggaagcaag atcgccgaat ccggatccac cggccgcgcc accggcccgc accttcactt
      481 tgaagtccgc taccgcagcg tcgcgcagaa cccgatccga ttccttcagg ccacggcgcg
      541 ctagccggca tttccgccgc gcacaggaag tctccatcgc gcggtttccc gcgcgtttcc
      601 atctcctcga gtcgcgggga gcagccgcgc cggcgtgata aactccgaac ctttgcactc
      661 cacggcagtc attcctagat gatttcccgc gcactgaagg cggtgttcgg cagccgcaat
      721 gatcggctgc tcaagcacta cggcaggtcc gtgcgggtga tcaacgctct cgagccgtcg
      781 atggcggcgc tcccggatca cggcctgggg gcgaaaacgc tcgagctcag ggagcgtttc
      841 gcaaagcggc tccaaggcgt ccccgaagag gaacgcgacg cggcggaaaa ggccgcgctc
      901 ctcgagctcc tcccggagtc cttcgccgtg gttcgcgagg cggcgaagcg cgtgctcaac
      961 atgcgtcatt tcgacatgca gctgatcggc ggcatggtcc tgcacgacgg caagatctcg
     1021 gagatgcgca ctggtgaagg caagacgctg gttgcaacct tgcccgcgta cctcaatgcc
     1081 ctctccggcc gtggcgtgca catcgtcacg gtcaacgact acctcgcgag ccgggacgcc
     1141 gagtggatgg ggaaggtcta caaattcctc gggctatcgg tgggggtgat tctgacccag
     1201 atgccgcacg ccgaaaaaca atcggcctac gccgccgaca tcacctacgg caccaacaac
     1261 gaatttggct tcgactacct gcgcgacaac atggcgatgc aggtgggcga ccgcttccag
     1321 cgcaagctga actacgcgat tgtcgacgaa gtcgactcga tcctgatcga cgaggcccgc
     1381 acgcctctca tcatttcggg gcgggccgag gaccgcaccg agatctacca gcgcatggac
     1441 aaggtggcgc cgatgctcaa gcgccaggag aaagaaagcg cgccaggtga tttctacgtc
     1501 gacgagaaga accacacggt actcctctca gaagaagggc acgagcacgc cgaggcgctc
     1561 ctcggccgcg cggggttgct gcccgaaggg cgcagtctct acgaaccggc gtacgttaac
     1621 ctcatgcatc acttgaacgc ggcgctccgg gcgcacaatc ttttctttcg cgaccggcac
     1681 tacgtggtcc aggacggcga agtgatcatc gtcgacgagt tcaccggccg gctcatgccg
     1741 gggcgtcgct ggtccgacgg cctgcaccag gcgatcgagg cgaaggagaa cgtgtcgatc
     1801 cagagtgaga accagacgct cgcgtcgatc acattccaga actacttccg catgtatcgc
     1861 aagctcgcgg ggatgacggg aacggccgat accgaggcct acgaattcca ggagatctac
     1921 gggctggagg tcgtcgtcgt cccgacgcac atgccgatga tccgggccga tcgtaacgat
     1981 caggtctttc ggaccgcagc ggaaaagtac aaggcgatca tcggcgatat caaggactgc
     2041 cacgagcgcg gccagccggt gctggtcggc acgacctcga tcgagaattc cgagctcatc
     2101 tcgggaatgc tggagaagga caagttgccg caccaggtcc tgaacgcgaa gcaacacgcg
     2161 cgcgaggcgg aaatcgtcgc ccaggccggg cgtccgaaga tgatcaccat agcgaccaac
     2221 atggcgggcc ggggcacgga catcgtgctg ggcggcaacg tcgagaagca gatcgatcta
     2281 gtgcgggaca aggacgaact cgatgccgcc gggaaggagc gtcgcatcag cgagctgcgc
     2341 catgagtggc aggacctgca taaccgcgtc atcgccgcgg gcggcctgca catcatcggg
     2401 accgagaggc acgaggcgcg gcgcatcgac aaccagctgc gcggccgctc cggccggcag
     2461 ggcgatccgg gctcctcgcg cttttacctt tcgctcgaag acccactgct gcggatcttc
     2521 gccggagagc gggtcaacgc catcatgcaa cgcctgggga tgcccgaggg tgagccgatt
     2581 gagcatagcc tcgtcactcg atcgattgag agcgcgcagc gcaaggtcga ggcgcacaac
     2641 ttcgacatcc gcaagcaact gctggaatac gacaacgtcg cgaacgacca gcgtcgcgaa
     2701 atttatcatt tgaggaattc catcctcgaa tcgcaggacg tttcggacca gatcgctgcg
     2761 ctgcgcgagg gcgttgtgac cgatttcttc cgcgcccatg tccccgaaga gagcatggaa
     2821 gagcaatggg acgttccggg ccttgaaaaa gtgtacgcgg ccgagctccg gctcgaactg
     2881 cctctttcga agtggctcga gagcgacccg ggcctggacg acgccgcgct gcttaagcgc
     2941 acgatcgacg aggcccacgc ggcctacgcc cgcaagatgg cagccaatcc gccggaggcc
     3001 gtgcatcacg tggaacgcct catactgctc tcgacgctgg acaaccactg gctcgagcac
     3061 ctcgccgccc tcgaccacct gcgccaggga atccatctgc gcggttacgc gcagaaaaac
     3121 ccgaagcagg aatacaagcg cgaggcattc gagctgttcg gcaacctcgt ggaatcggtc
     3181 aaactggaag tgacgcgcac gctgatgacc gtcgagctgc agatgcgcga gcaggtcgag
     3241 caggtagaga agctgcaggc gccagcgctc gagaacgtgc aataccagca cgccgggtac
     3301 gaagaagccc tcgccggagc gggtgcagat gcggccccgg cgtcgaagcc ggcgcctttc
     3361 gtcaggcaga tggggaaaat cggtcgcaac gatccttgtc cctgcgggtc ggggaagaaa
     3421 tacaagcact gccacgggaa gctgagctag caggggagcg gcgcccaacc taatggctgt
     3481 caatttgagc cctcctcggc cggaggactt gttgctcgtc aagggcgtca agctcggcgt
     3541 cgccgaggcc ggaatccgca aggccaggcg caaggacctg ctcctcatga cgctcgcgga
     3601 gggcacgtcc gcctcgggcg tgttcaccca gaatcgcttt tgcgcagcac cggtccaagt
     3661 ggcgaaggag cacttggcta ccgggtcacc ccgggcgctg ctcgtcaata ccggcaacgc
     3721 caacgccggt accggggagg ccgggattgc cggcgcaagg gcgagctgcg atgcggccgc
     3781 ccggctcgtc ggctgcgaat cccgccgggt tttgccgttt tcgacgggcg tgatcatgga
     3841 gcccctgccc gtcgagcgca tcgtcgcggg cctgccggcg tgcgtcgccg acctgcgcga
     3901 ggacaactgg gcgcgggcag ccgaggcgat catgacgacc gattcggtgc cgaaagcgcg
     3961 gtcgcggcgc gtgcagctcg cggggggcga ggtgaccgtc accgggatcg cgaaaggcgc
     4021 gggcatgatc cggcccgacc tggcgacgtt gctcgcgttc gtcgcgacgg acgctcccgt
     4081 cgctcaaggt gcgctgcgcc agctcctcgc cgatgccgcg gcgcgctcgt tcaaccgcat
     4141 cagcgtggac ggcgatacgt ccaccaacga ttccttcgtc ctgattgcaa ccggcgcgag
     4201 cggcgggacg atgatcgacg acccgggcca tcccggattc gcgccgttgc gggaagcggt
     4261 gctcgaggta tcgcaggggc tcgcgcaggc gatcgtgcgc gacggcgaag gcgcgaccaa
     4321 gttcatcacc gtgcgcgtgg aaaaaggcgg tgacgaggcg gaatgcgcga gcgtcgccta
     4381 cgcgatcgcg cactcgcctc tggtgaaaac cgcgttcttc gcctccgatc cgaatctggg
     4441 gcggctcctc gccgcggtcg gaaacgcagg cgtgcgcgat ctggaggtcg gccgcgtcga
     4501 actgtacctc gacggggtgc tggtgtcgaa cgacgggggc cgcgccccgt cttatcgtga
     4561 agaggacgga aagcgcgtaa tggcgaaaag cgagatcacg gttcgcgtcg tgctcaaccg
     4621 cgggcttgcg gcgaccacgg tgtggacctg cgacctgtcg cgcgattacg ttactatcaa
     4681 cgccgaatac agaacctgag acgcgccgat gtctgagctc gaacggttga tcaagcgagg
     4741 cgaagcgatc ctcgagcgcg tggaaacgct gctgcc
//
LOCUS       DASPZD010000233         4462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1702713, whole genome shotgun sequence.
ACCESSION   DASPZD010000233 DASPZD010000000
VERSION     DASPZD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702713"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VEM33_04490"
     CDS             <1..217
                     /locus_tag="VEM33_04490"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HYS51112.1"
                     /translation="AGDGAELERLYSVRVGGSRQRWTLALTPRELEVAGVVKSIGIAG
                     SGSRIVRLEILEPGGDGSVMTIHEKSP"
     gene            217..2547
                     /locus_tag="VEM33_04495"
     CDS             217..2547
                     /locus_tag="VEM33_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYS51113.1"
                     /translation="MPYRRTALLTWLAIVAASAWWTARHVVVTSDLTAFLPAASGRLE
                     RLLVEQLRAGVASRTMLVAIEGGAPPELARFSRELARAIALDRRFDYVMNGAAEFTRG
                     ARELGLKHRYLLSPTVSAGRFSEQSLARELRRNLERFATLEGTAFKAMIASDPTGELR
                     SLAETAGQGAPASRHGVWFSKDGARALLVAQTAASGIDAVRQQEAADALADAFRQTGA
                     GPEYRVLITGPGVFAARTRAAIEHDTRWLTGLAGAVVLLILVLVYRSPGPVALSALPV
                     LTGLVVGVAAVGALFGSVHGITLAFGATVIGEAVDYPSYVFTQRKAGESLHDILARVW
                     PTLRLAVLTTVFGSLALLLSSLQGLSQLGVLSMVGVLAAGLVTRWVLPALAPGRGAPP
                     PRTLPFSLVRAGLALRSVAWIAWMLLAAALAFTVARHDRIWDDDLGHLSPLPESARQL
                     HRQLSDDLRAPDVGYLLIATAASREAALEKGERLTPALQSWVAEGVLEGFDLAVRYLP
                     SAKTQALRRAALPEAQDLHRSLARALRGLPYRADAFAPFERDVEAARTGPLLTIEDLR
                     GTGLDLKVQTLLASSGGEWHALIPLRGMRDAARVRSLAAQLPEETRASFLDLKGESDR
                     LIGGYRSQALELAGLGAVAIAVLLWFGLRGVVKAVRVMLPVYAAIAMTVACLMLLGIG
                     LSLFHLVSLLLVLGIGLNYSLFFSRAQPGTDSEQGTSLALSVCFLTTFAAFGCLATSR
                     IPVLSAIGLTVTLGCALSLLNSMMLGGTAAQEREAR"
     gene            2594..4381
                     /gene="aspS"
                     /locus_tag="VEM33_04500"
     CDS             2594..4381
                     /gene="aspS"
                     /locus_tag="VEM33_04500"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYS51114.1"
                     /translation="MRTHYCGKLSAALLGQTVTLAGWAHRRRDHGGVIFIDLRDREGL
                     AQVVSDPDRKATFATAERIRNEFVLKVTGLVRRRPEGTVNPNIASGEVEVLAHSIEIL
                     NASLPPPFQLDDDNLSETVRLTHRVIDLRRETMQKNMMLRYRVAMAVRKYLDGNGFID
                     IETPMLTRSTPEGARDYLVPSRVHAGEFFALPQSPQLFKQLLMIAGFDRYYQITRCFR
                     DEDLRADRQPEFTQIDCETSFLDETEIRAIFESMIRSVFKEALDVALPDPFPVMSYGD
                     AARDYGSDKPDLRVPLKLTELTEVMKSVEFKVFSGPANTPAHRVAGFLVPGGGELTRG
                     EIDAYTEFVAIYGAKGLAWIKFNDVAKGRDGMQSPIVKNLSDAALRAIVDRSGTKNGD
                     LMFFGAGKIKVVNDALGALRVKIGHEKGYARGGWKPLWVVDFPMFEYDEEAKRWNAMH
                     HPFTAPKDSHEDLLARDPGAALAKAYDMVLNGAEIGGGSVRIHREEVQSKVFRALSIG
                     PDEAKHKFGFLLEALQYGAPPHGGIAFGLDRIVAMMAGTESIRDVIAFPKTQRAQDLL
                     THAPSPVDEKQLRELHIRLRSPAVTTPTA"
BASE COUNT          661 a         1552 c         1487 g          762 t
ORIGIN      
        1 tgccggcgac ggcgcggagc tcgagcggct ctattccgtc agggtgggag gttcgaggca
       61 gcgctggacg ctggcgctta ccccgaggga gctcgaggtc gcgggcgtcg tgaaaagcat
      121 aggcattgcc ggctcgggtt cgcgcatcgt tcgtctcgaa atcctggaac cgggtggaga
      181 cggatcggtg atgaccatcc acgaaaaatc tccgtgatgc cctatcggcg cacggcgctg
      241 ctcacctggc tcgcgatcgt tgctgcgagc gcctggtgga ccgcgcgcca cgtggtcgtc
      301 accagcgatt tgacggcctt cctcccagcc gccagcggcc gcctcgagcg gctcctcgtc
      361 gagcagctgc gcgcaggcgt ggcctcccgc accatgctgg tcgcgatcga aggcggcgcg
      421 ccgcctgagc tcgcgcgctt ctcccgcgag ctcgcgcgcg cgatcgcact cgaccggcgc
      481 ttcgactatg tcatgaatgg cgccgctgaa ttcactcgag gcgcgcgcga gctcggattg
      541 aagcatcgct atctgctcag cccgaccgtg agcgccgggc gcttcagcga gcaatcgctc
      601 gcccgcgagc tgcgcaggaa cctcgagcgg ttcgcgacgc tcgagggaac ggcgttcaag
      661 gcgatgatcg cgagcgatcc gacgggcgaa ctgcgcagcc tcgccgagac cgcgggtcag
      721 ggcgcgcccg catcccggca cggcgtctgg ttctcgaaag acggcgctcg cgcgctcctt
      781 gtggcgcaga ccgcagcctc cggaattgat gccgtgcgcc agcaggaggc ggctgacgcc
      841 ttggcggacg cctttcgaca aaccggagcg ggtcccgaat atcgcgtgtt gatcaccggg
      901 ccgggcgtgt ttgccgcacg cacgcgcgcg gcaatcgagc acgacacccg ctggctcacg
      961 ggccttgccg gcgcggtggt cctcctgata ttggtccttg tataccgttc ccccggcccc
     1021 gtggcgctct ccgcgctgcc ggtgctgacg ggcctcgtcg taggagtcgc ggcggtgggc
     1081 gcgctgttcg gcagcgtgca cgggatcacg ctcgccttcg gcgcgaccgt gatcggcgag
     1141 gcggtcgact acccgtccta cgtcttcacg cagcgcaagg ccggcgaatc actccacgac
     1201 atcctggcgc gagtctggcc gaccttgcgc cttgcggtgc tcaccaccgt cttcggttcc
     1261 ctcgcgctgc tgctctccag cctccagggc ttgtcgcagc tcggcgttct gtcgatggtc
     1321 ggcgtgctcg cggcggggct cgtcacgcgg tgggttctgc ccgcgctcgc gccgggccgg
     1381 ggagcgccgc ccccgcgcac gctgcccttc agcctggttc gcgcgggact cgcgttgcgc
     1441 agcgtggcgt ggatcgcctg gatgctgctc gccgctgcgc tcgctttcac cgtcgcgcgc
     1501 catgaccgga tctgggacga cgacctcggc cacctgagcc ccctgcccga atcggcgcgc
     1561 cagctgcacc ggcagttgag cgacgatctg cgcgcgcccg acgtgggcta tctcctgatc
     1621 gcgacggccg cgagccgcga ggcggcgctc gagaagggcg agcgactgac gcccgctctc
     1681 cagagctggg tggccgaggg cgtgctggaa ggcttcgatc tggcggtgcg ctatctgccg
     1741 agcgcgaaga cccaggccct ccgccgcgct gcgctgcccg aagcccaaga cctgcaccgc
     1801 tcgctcgccc gggcgcttcg aggcctgccg tatcgcgccg acgcctttgc gccgttcgag
     1861 cgcgacgtgg aagcggcgcg caccgggcct ttgctcacga tcgaggacct gcgcggcacc
     1921 gggctcgacc tcaaggtgca gactctgctc gcctcgagcg gcggcgaatg gcatgctctc
     1981 attccgctgc gcggcatgcg cgacgcggcg cgggtgcgct cgctcgccgc gcagttgcca
     2041 gaggaaacgc gcgcgagctt cctcgacctg aagggcgaat cggaccggtt gatcggcggc
     2101 taccgctccc aggccctcga gctcgccggc ctgggcgcgg tcgcgatcgc cgtgctgctc
     2161 tggttcgggc tgcgcggcgt ggtcaaggcg gtgagggtca tgctgcccgt gtatgcggcg
     2221 attgcgatga cggtcgcctg cctcatgctg ctcgggatcg gcttatcgtt gttccacctg
     2281 gtttcgcttc tgctcgtgct cggcatcggt ctcaactact ccctgttttt cagccgcgcg
     2341 caacccggaa ccgatagcga gcagggcacc tcgctcgcct tgtcggtgtg ttttctcacc
     2401 acgttcgccg cgttcgggtg ccttgcgacg tcgcgcatcc ccgtactgtc ggcgatcggg
     2461 ctcaccgtca ccttgggctg cgccctgtcg cttctcaact cgatgatgct cggcggcacg
     2521 gcggcgcaag aacgcgaagc gcgctaggcg cgaccgattc ccgcgggtat cggtttgcta
     2581 tactccgccc cccatgcgca cccactattg cggcaagctc tccgcggcgc tcctagggca
     2641 gaccgtgacg ctcgccggct gggcgcaccg ccgccgcgac cacggcgggg tcatcttcat
     2701 cgacctgcgg gaccgcgagg gcctcgcgca ggtggtgtcg gatccggacc gcaaggcgac
     2761 tttcgcgacg gcggaacgga tacgcaacga gttcgtgctg aaagtcaccg gcctcgtgcg
     2821 ccggcgtccc gaaggcaccg tcaatccgaa catcgcctcg ggcgaggtcg aagtgctcgc
     2881 gcacagcatc gagatcctca acgcctcgct ccctccgcct ttccagctcg acgacgacaa
     2941 tctctccgag accgtgcgcc tcacgcaccg cgtgatcgac ctgcgccgcg agaccatgca
     3001 gaagaacatg atgctgcgtt accgcgtggc gatggcggtg aggaaatacc tcgacgggaa
     3061 cggattcatc gacatcgaga cgccgatgct tacgcgctcc acgcccgagg gcgcgcgcga
     3121 ctacctcgtg ccctcgcgcg tgcacgcagg cgagttcttc gcgctgccgc aatcgccgca
     3181 gctcttcaag cagctcctta tgatcgcggg atttgatcgt tactaccaga tcacccgatg
     3241 cttccgcgac gaggacctgc gcgccgaccg ccagcccgag ttcacccaga tcgattgcga
     3301 gacttccttc ctcgacgaga cggagatccg cgcgattttc gagagcatga tccgaagcgt
     3361 tttcaaagag gccctggatg tcgctctgcc cgacccgttc ccggtgatga gctatgggga
     3421 cgcggcacgc gactacggct cggacaagcc ggacctgcgc gtgcccctga agctcaccga
     3481 gctgaccgag gtgatgaagt cggtggagtt caaggtgttt tccgggccgg ccaacacgcc
     3541 cgcgcaccgg gtcgccggat tcctcgtccc cgggggcggg gagctcacgc gcggcgagat
     3601 cgacgcttac accgagttcg tcgccatcta cggcgcaaaa ggcctcgcct ggatcaagtt
     3661 caacgacgtc gccaagggcc gcgacggcat gcagtcgccc atcgtgaaga acctgtcgga
     3721 tgcagccttg cgcgcgatcg tcgatcgctc cggcaccaaa aacggcgacc tgatgttctt
     3781 cggcgcggga aaaatcaagg tcgtcaacga cgcgctcggc gccttgcgcg tgaagatcgg
     3841 ccacgagaag ggctacgcgc gaggcggctg gaagccgctc tgggttgtcg actttccgat
     3901 gttcgagtac gacgaggagg cgaagcgctg gaacgcgatg caccacccgt tcaccgctcc
     3961 caaggacagc cacgaggatc ttcttgcgcg cgatcccggg gcggcgctcg ccaaagcgta
     4021 cgacatggtg ctaaacggcg cggagatcgg cgggggctcg gtgcgcatcc accgcgagga
     4081 agtgcagtcg aaggtgttcc gcgcgctgtc gatcgggccc gatgaagcga agcacaagtt
     4141 cgggtttctg ctcgaagctc tgcaatacgg tgcgccgccg cacggcggga tcgccttcgg
     4201 cctggaccgc atcgtcgcca tgatggccgg caccgagtcg atccgcgacg tcatcgcctt
     4261 tcccaagacg cagcgcgcgc aggacctgct cacgcacgcg ccctcgccgg tcgacgagaa
     4321 gcagctgcgc gagctgcaca tccgcctgcg cagcccggcg gtgacgacgc cgacggcctg
     4381 agtcctccgc tgacctgacc ggccgcgtgc cggttcgagg cccgcaccgt tcgcttcggc
     4441 ggatcgtgcg ggctttttcg tc
//
LOCUS       DASPZD010000234         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1683831, whole genome shotgun sequence.
ACCESSION   DASPZD010000234 DASPZD010000000
VERSION     DASPZD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683831"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..526)
                     /gene="nusB"
                     /locus_tag="VEM33_04505"
     CDS             complement(104..526)
                     /gene="nusB"
                     /locus_tag="VEM33_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYS51115.1"
                     /translation="MKSARRRSRELALQGLYAWQLAGDDAAGLQSQLAESKSFGKADA
                     EYFARLLQGTIAHAASLERLIAPLLDRKLKELSPVERGILLLAAFELKNATDIPYKVV
                     INEAIELAKDFGGTDGHKYVNGVLDKLVSELRPQERKA"
     gene            complement(523..981)
                     /gene="ribH"
                     /locus_tag="VEM33_04510"
     CDS             complement(523..981)
                     /gene="ribH"
                     /locus_tag="VEM33_04510"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYS51116.1"
                     /translation="MAEIGPDSSGEGLRVGIVRSRFNERIGVAMLEACRKRLAELRVT
                     ALTVISVPGALEAPLALQKLANTERYDALIALGAVIRGETHHFDVVANESASGLMQVQ
                     LDSGIPVANGILTTDTEEQAKARAAQKGRDCAEVAVEMANLSKSIAKSHK"
     gene            complement(981..2090)
                     /gene="ribBA"
                     /locus_tag="VEM33_04515"
     CDS             complement(981..2090)
                     /gene="ribBA"
                     /locus_tag="VEM33_04515"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HYS51117.1"
                     /translation="MSLNSIQEIIAELKAGRMAVLVDDEDRENEGDLIFAAEFVTAEK
                     INFMAKFGRGLVCMPITEAHAERLNLLPMVARNRSVHGTNFTVSIEAATGVTTGISAA
                     DRAHTIKVAASPKATPADIVQPGHVFPLIARAGGVLVRAGHTEACCDLAQLAGLHPAA
                     VLCEIMKDDGSMARLPDLIEFSKHHGLKIGSIADLIHFRSQNESLIKRVTERAIETRF
                     GPFRLVAYLEKISGETQLALVRGEIVPDKETLVRVHAPLSVLDLIEAGPRAHSWSVPD
                     ALERIAAEGKGVMVLLNCAETASRLIERVASPERTEGPSKMDFLTYGIGAQILRDLRV
                     GKMRLMASPRKMPSMAGWELEIAGYVEKAAKARDN"
     gene            complement(2282..2659)
                     /gene="rplS"
                     /locus_tag="VEM33_04520"
     CDS             complement(2282..2659)
                     /gene="rplS"
                     /locus_tag="VEM33_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYS51118.1"
                     /translation="MNIIQKLEEEEIKRMGKKLPEFSPGDTVIVNVGVVEGERKRLQA
                     YEGVVIAKRNRGINSSFTVRKVSSGEGVERTFQTYSPMIDSIELKRKGDVRRAKLYYM
                     RKLSGKGARIREKIEGHEQGGEQ"
     gene            complement(2656..3408)
                     /gene="trmD"
                     /locus_tag="VEM33_04525"
     CDS             complement(2656..3408)
                     /gene="trmD"
                     /locus_tag="VEM33_04525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYS51119.1"
                     /translation="MRFDCVTLFPEAFAAVTDSGVTRRALEEGLWSLSLWNPRDFTTD
                     NYRTVDDRPYGGGPGMVMLAEPLEKAIAAARAAAPGEAKVIYLSPQGPVMDHRKVMEL
                     AQGARWVLLCGRYEGVDERLIRRRVDGELSIGDYVLSGGELAAMVLIDAVVRQLPGAL
                     GDGLSAEQDSFVNGLLDCPQYTRPETYAGEAVPPVLLSGHHAQIERWRLKQSLGRTWL
                     RRPELLERRGMSEGERKLLEEFRQELDEGAKR"
     gene            complement(3408..>3599)
                     /locus_tag="VEM33_04530"
     CDS             complement(3408..>3599)
                     /locus_tag="VEM33_04530"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HYS51120.1"
                     /translation="LAVVNAQGERLGKVTGFFSAGGANDVMRVEHEGGERLVPATAEV
                     IRKVDLAAGTVEVDWEADW"
BASE COUNT          542 a         1259 c         1131 g          667 t
ORIGIN      
        1 gtcgggccgg aggagcgcgc agtcgtcgcc cacccccagg gccgccgtcg gtgcgtcccg
       61 ggtgaagtat ttgcggatga tgtcgaactc ggaggccacg tcttcaggct tttctctcct
      121 gcgggcgcag ctcggagacg agcttgtcga gcacgccgtt cacgtacttg tggccgtcgg
      181 tgccgccgaa gtctttggcg agctcgatcg cttcgttgat caccacctta tagggtatat
      241 cggtcgcgtt cttgagttcg aacgccgcga gcagcaggat gccgcgctcc accggcgaga
      301 gctccttcag cttgcgatcg agcaagggcg cgatcaggcg ctcgagcgag gcggcgtgcg
      361 ctatcgtgcc ctgcaggagc cgcgcgaaat actcggcgtc ggccttacca aagctctttg
      421 attccgccaa ctgggactgg aggccggccg cgtcgtcgcc cgcgagctgc cacgcgtaca
      481 gaccttgcag cgctagctcg cgcgaacggc gccgcgccga tttcatttat ggctcttcgc
      541 gatgctcttg gagagattcg ccatttccac cgcgacctcg gcgcagtccc ggcctttttg
      601 cgcagcgcgc gccttcgcct gttcttcggt gtcggtggtg agaatgccgt tcgcgaccgg
      661 gatgccgctg tcgagctgca cctgcatgag ccctgaagcg gattcgttcg caacgacgtc
      721 gaaatggtgc gtctcgccgc gtatgaccgc accgagcgcg atcagcgcgt cgtacctttc
      781 cgtgttggcg agcttctgca gggcgagcgg cgcctcgagc gcgcccggca cgctgatcac
      841 ggtgagggcg gtgacgcgca attccgcgag ccgcttgcgg caggcttcca gcatcgcgac
      901 gccgatgcgc tcgttgaagc gtgagcgcac gatcccgacg cgcaggcctt ccccgctcga
      961 gtcgggcccg atttccgcca tcagttgtcc cgcgccttgg cggccttttc gacgtagccc
     1021 gcgatctcca gctcccaccc ggccatgctc ggcatcttgc gtggcgaggc cataaggcgc
     1081 atcttgccga ccctgagatc gcgcaggatc tgcgcgccga ttccgtacgt gaggaaatcc
     1141 atcttggacg gtccttcggt ccgctcgggc gatgcgacgc gctcgatcag ccgggacgcg
     1201 gtctccgcac agttgagcag caccatcacg cccttgccct cggcggcgat gcgctcgagc
     1261 gcgtcgggca ccgaccagga gtgcgcccgc ggcccggcct cgatcaggtc gagcacggag
     1321 agcggcgcgt gcacgcgtac cagcgtttcc ttgtcgggca cgatctcccc ccggacgagc
     1381 gcaagctggg tttctccgct gatcttttcc aggtaggcga cgagccggaa cggcccgaag
     1441 cgggtctcga tcgcgcgctc ggtcacgcgc ttgatgagcg attcgttctg gctcctgaag
     1501 tggatgagat cggcgatcga gccgatcttg agcccgtgat gcttcgagaa ctcgatcaag
     1561 tcgggcagcc gcgccatgct tccgtcgtct ttcatgatct cgcacagcac cgccgccgga
     1621 tggagtccgg cgagctgcgc gaggtcgcag caggcctcgg tgtgcccggc gcgcaccagc
     1681 accccgcctg cccgcgcgat gagcgggaac acgtgacccg gctggacgat gtccgcgggc
     1741 gtcgccttgg gggaagccgc caccttgatg gtgtgagccc ggtcggccgc ggaaatgccg
     1801 gtggtcacgc cggtggccgc ttcgatggac acggtgaaat tggtgccgtg caccgagcgg
     1861 ttgcgcgcca ccatcggcaa gaggttcagg cgctcggcgt gcgcctcggt gatcggcatg
     1921 cagacgaggc cgcggccgaa cttcgccatg aaatttattt tctccgcggt gacgaactcc
     1981 gcggcgaaga tgaggtcgcc ctcgttctcg cgatcctcgt cgtcgacgag cacggccatg
     2041 cggccggctt tcagctcggc gatgatttcc tggatcgagt tgaggctcat gatgtcgtcc
     2101 gtccgagcgc gaattttacg ccgttccgag aaggtggtcg gcagtacctt cccctccttg
     2161 gataaggagg ggtggcgcga agcgccgggg tggtttgatg gagaggcgcg ctaccacccc
     2221 gccccttcgg ggcacccctc ctcaaacgag gaggggaaag agcgtcgatt cgcgacgcgg
     2281 cctactgctc gccgccctgc tcgtggcctt cgattttctc gcggatgcgc gcgcccttgc
     2341 ccgagagctt gcgcatgtag tagagcttgg cgcggcgcac gtcgcccttc ctcttcagct
     2401 cgatcgaatc gatcatcggc gaataggtct ggaaagtgcg ctcgacgccc tcgcccgagg
     2461 acaccttgcg caccgtgaac gaggaattga tgccgcggtt cctcttggcg atcaccacgc
     2521 cctcgtaagc ctgcaagcgc ttgcgctcgc cttcgacgac gccgacgttg acgatcaccg
     2581 tgtcgcccgg ggaaaactcg gggagcttct tccccatgcg cttgatttct tcctcttcga
     2641 gcttttgaat gatgttcatc gcttcgctcc ttcgtccaat tcctgcctga actcttcaag
     2701 cagtttgcgt tcgccctcgc tcatcccgcg ccgctcgagc agctcgggcc tcctgagcca
     2761 cgtgcggccg agcgactgct tcagccgcca gcgctcgatc tgcgcatggt ggccggagag
     2821 cagcaccggc ggcaccgcct cgcccgcgta cgtttccggc cgcgtgtact gcgggcagtc
     2881 gaggagcccg ttcacgaacg agtcctgctc ggccgacagc ccgtccccga gcgctccagg
     2941 cagctggcgc accaccgcgt cgatcagcac catcgcagca agctcgccgc ccgagaggac
     3001 atagtcgccg atcgacagct ccccgtccac tcggcggcgg atcagccgct cgtccacgcc
     3061 ttcgtaacgc ccgcacagca acacccaccg cgccccctgc gccagctcca tcaccttgcg
     3121 gtggtccatc accggccctt ggggcgagag atagatcact ttcgcctcgc ccggcgccgc
     3181 ggcccttgcc gcggcgatcg ccttctccag cggttcggcg agcatcacca tgccgggccc
     3241 gcccccgtac ggccggtcgt cgaccgtgcg gtaattgtcc gtcgtgaaat cgcgcggatt
     3301 ccacagcgag aggctccaca gcccctcttc cagcgcccgg cgcgtcacgc ccgagtccgt
     3361 gaccgccgcg aacgcctcgg gaaacagcgt cacgcaatcg aagcggatca ccagtccgcc
     3421 tcccaatcga cttcgaccgt accggcggcc agatcgacct tgcggatcac ctccgcggtc
     3481 gccggcacga ggcgctcccc gccttcgtgc tcgacgcgca tcacgtcgtt cgcgccgccg
     3541 gcgctgaaaa atcccgttac cttgcccaag cgctcgccct gagcgttcac caccgccag
//
LOCUS       DASPZD010000235         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_464629, whole genome shotgun sequence.
ACCESSION   DASPZD010000235 DASPZD010000000
VERSION     DASPZD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464629"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEM33_04535"
     CDS             <1..392
                     /locus_tag="VEM33_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYS51121.1"
                     /translation="LDLNTWVGMALQRAIDVISNRLQSGLAFLATVGSTAPFVGLFGT
                     VWGIYHALTAIGIAGQASIDKVAGPVGEALIMTALGLAVAVPAVLGYNWLVRRNRVAM
                     ELIRHFGGGVDMLLLGRGGRMRRDSES"
     gene            389..826
                     /locus_tag="VEM33_04540"
     CDS             389..826
                     /locus_tag="VEM33_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYS51122.1"
                     /translation="MSMNVANPDAEDDLVADINTTPLVDVMLVLLIIFLITIPVVIQS
                     VPVELPKERNQPTQTKPENIVIAVNKDGDVFWNNQLIAEEKMLLDRLREVAVRQPQPE
                     IHIRGDKEARYESIGRVVFACQRIGILKIGFITEPPARTGVRG"
     gene            834..1265
                     /locus_tag="VEM33_04545"
     CDS             834..1265
                     /locus_tag="VEM33_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYS51123.1"
                     /translation="MAMKIGSGSGTHDPDVMVDINTTPLIDVMLVLLIMLIITIPIQT
                     HAVKLNMPIGTPPPPPVPPEVVTIEVDFDGTLIWNGRVVPDRQELEERLRAAAEMPDQ
                     PEVHLRPNKLVKYESVAMVMASAQRLGLKKIGLIGNEQFMR"
     gene            1288..2532
                     /locus_tag="VEM33_04550"
     CDS             1288..2532
                     /locus_tag="VEM33_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51124.1"
                     /translation="MRVRFRFMRLCFIGLCSIALFAAPALAQEPGAGAQTVRPEIGKP
                     IQAAIELIKSKKGKEAIAKVREAQAVGDRTPYENYLVERVLGQAAATAGDASTAARAF
                     ENVATMPGTPQGERQPFLAAAASQYYVVKNYAKTAELAARYFKDGGTEKSMRTIYVQA
                     LYLGNDFAGAAKELTKDLEAEEQAGRKPTEELLQLLANSCTQIKDAACYGKAMEMLVA
                     HYPKREYWLSVIYGIATRPGFSERLALDLARIKLETGTVRSADEYLDAAQLALQEGFP
                     MDAVRIIDKGYASGMLGTGAEAARHKRLKDMAGKNLADDKKALAREESGGAKDGRALF
                     NEGFNYVLLGKAEQGLALMEKGIRDAKAFRRLDHAKLQLGYAYHLAGQNQKAIQILKT
                     VQGTDGAAALARLWAVRLSHAS"
BASE COUNT          513 a          816 c          857 g          390 t
ORIGIN      
        1 agctcgatct caatacctgg gtcggcatgg cccttcagcg cgcgatcgac gtgatcagca
       61 accgcctgca gagcggccta gcgttcctcg cgacggtggg ctcgaccgcg ccattcgtcg
      121 gcctgttcgg gaccgtatgg ggcatctacc atgcgctcac cgcgatcggc atcgcggggc
      181 aggcatcgat cgacaaggtc gcaggtccgg tgggcgaggc gctgatcatg accgcgctcg
      241 gcctcgcggt ggccgtgccg gcggtgctcg gctacaactg gctggtccgc cgcaacaggg
      301 tggccatgga gctgatccgg cacttcggcg gaggcgtcga tatgttgctg ctcggccgcg
      361 gcggccgcat gcgcagggac tccgaatcat gagcatgaac gtcgccaacc cggacgccga
      421 agacgatctc gtcgcggata tcaacacgac cccgctcgtc gacgtgatgc tcgtattgct
      481 gatcatcttc cttatcacga tccccgttgt gatacagagc gtgcctgtcg agctgccgaa
      541 ggaaagaaac cagccgaccc agaccaagcc cgagaacatc gtcatcgccg tcaacaagga
      601 cggcgacgtg ttctggaaca atcagctcat cgcggaggaa aagatgctgc tcgaccggct
      661 gagggaagtc gcggtgcgcc agccccagcc ggaaatccac attcgcggcg acaaggaggc
      721 gcgctacgag tccatcggca gggtggtgtt cgcgtgccag cgcatcggga tcctgaagat
      781 cggtttcatc acggagccgc cggcgcggac cggcgtgcgg gggtgacgca ctcatggcca
      841 tgaaaatcgg atccggaagc ggaacgcacg acccggacgt gatggtggac atcaacacga
      901 ccccgttgat cgacgtgatg ctggtcctgc tcatcatgct catcatcacg attcccatcc
      961 agacgcacgc agtcaagctg aacatgccga tcggcactcc cccgcccccg ccggtccctc
     1021 ccgaggtggt gacgatcgag gtcgatttcg acggcaccct gatctggaac gggcgggtcg
     1081 tgccggatcg ccaggagctc gaggagcggt tgcgcgcggc cgccgaaatg ccggatcagc
     1141 cggaggtgca cctgcgcccc aacaagctgg tcaaatacga gagcgtggcg atggtgatgg
     1201 cttcggcgca acgcctgggg ttgaaaaaaa tcggcctgat cggcaacgag cagttcatgc
     1261 gctgaaagcg ccgagcggcg aattcacatg cgcgtccgtt ttcgcttcat gcgcctctgc
     1321 ttcatcggtc tttgctcgat cgccttgttc gccgcgcccg cgctcgcgca ggagccgggc
     1381 gcgggcgccc agacggtgcg acccgagatc ggcaagccca tccaggccgc gatcgagctg
     1441 atcaaatcca agaagggcaa ggaagcgatc gccaaggtgc gcgaagccca ggccgtcggc
     1501 gacaggacac cgtacgagaa ttacctcgtc gagcgcgtcc tggggcaggc cgcggccacc
     1561 gcgggggacg cgtccacggc ggcgcgcgcc ttcgagaacg tggcgaccat gccgggcacg
     1621 ccccaggggg aaaggcagcc gttcctcgca gccgccgcaa gccaatacta tgtggtgaaa
     1681 aactacgcaa aaaccgccga gctcgcggcg cgatacttca aagacggcgg cacggaaaag
     1741 tcgatgcgca cgatttacgt ccaggcgctc tacctcggca acgacttcgc gggcgccgcc
     1801 aaggagctga cgaaggacct ggaagccgag gaacaggccg gcaggaagcc caccgaggag
     1861 ctgttgcagt tgctcgcgaa ttcctgcaca cagatcaagg acgcagcgtg ctacggcaag
     1921 gcgatggaaa tgctcgtcgc ccactacccg aagcgcgaat actggctgag cgtcatctac
     1981 ggcattgcga cgcgtcccgg gttttccgag cggctcgccc tcgacctcgc ccgcatcaag
     2041 ctcgagaccg gcacggtgcg cagcgcggac gagtacctgg acgcggcgca gctcgcgctc
     2101 caggaaggat tcccgatgga tgcggtgagg atcatcgaca agggctacgc gtccggcatg
     2161 ctgggcaccg gggccgaagc cgcgcgccac aagcgcctga aggacatggc ggggaagaat
     2221 ctcgccgatg acaagaaagc gctcgcgcgc gaggagtccg gaggcgcaaa agacggcagg
     2281 gcgctattca acgaaggctt caattacgtc ctgctcggca aggcggaaca gggcctcgcg
     2341 ctgatggaga aggggattcg cgacgcaaag gccttcaggc ggctggacca cgcgaagctc
     2401 cagctggggt acgcctacca tctggcgggc cagaaccaga aggcgatcca gatcctgaag
     2461 acggtgcagg gaacggacgg cgccgcagcg ctggcccgct tgtgggcggt gcgtttgagc
     2521 cacgcatcct gatttcagtg cgcggcgctg cgcgccttct cgcggtggcg gcgcac
//
LOCUS       DASPZD010000236         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1211767, whole genome shotgun sequence.
ACCESSION   DASPZD010000236 DASPZD010000000
VERSION     DASPZD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211767"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VEM33_04555"
     CDS             <1..875
                     /locus_tag="VEM33_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYS51125.1"
                     /translation="SQGNRPPSPVELGCADPNSPCHLPNAMTSDPPLKQVVSRTLEAG
                     ARARTASGLRWNAALYRTVSYDDILFVSSSASSGFFTNFGKTRRQGVEAALERSEGKF
                     SWALNYSLIDATYQSSAVLFSQANSTADANGDIQVSPGDRIPGIPRHHLNASATYDLT
                     DRTTVGAGAVAVSRQFARGNDNNRHEPDGVNYLGPGEIAGYTIFNVNLDYKIDRGLRA
                     FAKVSNVFDRRYATAGALRQNFFPGGSLVAPGAQVNETFYAPGAPRALWIGIQLVSDA
                     AGARQSREAQASRD"
     gene            929..1237
                     /locus_tag="VEM33_04560"
     CDS             929..1237
                     /locus_tag="VEM33_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51126.1"
                     /translation="MPFPGRSTLAILFCGLLFATAAQAQRAKPQGFPDAPGKDVLVGK
                     CFQCHAPTMWQDHRQDRRGWEGVLYRMVGRGALWTEDEIKTMADYLAAAYGRQPAASK
                     "
     gene            1260..3179
                     /locus_tag="VEM33_04565"
     CDS             1260..3179
                     /locus_tag="VEM33_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYS51127.1"
                     /translation="MRELVARYLSRSISRRGFLKGLTTAGISLASAESILESLVPIAH
                     AQEAQRIAPEAIKMVEGTGAECFAEQLIASGVKYVFGNSASEDAQFYEALVDRPQLKY
                     ILTPHEGPGAAMAAGYVKASGEPTIVMQAAVVGLVNAMGQMFNAFKEQTPLVFYSYRT
                     DQTRRAGRDGFEEVANQEQIVQPMTKYSWLARRAEMIPETVRRGFKAAWTPPCGPTYV
                     SWHSDLNDEKTYAEIVTQDKVDPRMRVRPNPREVERAAKLLVEAKMPLMIVGDEVCGA
                     KAASKAVELAELLGMPVTQARQIYANFPESHPLWVGGPPATRIESLDFPKNPDVVMNV
                     GNKLQHNSPQPIVAREAKFIDMRSDSWSMGNVMNTEVPLVADVAYGLDDLRAAVEGIM
                     TSAMHRKARERAQEVRRFSEKAKALRALVAKNPEWDRSPMLADRVTSEVARFADRDAI
                     IVHEAGSVVLHGFDFDPLGGRELFFYYGAHLGSGVGTAAGVKLARPNQQVICLVGDGS
                     FVFGPTALWNMARLELPVITVVYNNHAYSGPHSRVIEKTAGGKMVQKRQFVHDYLGSP
                     DMNMAQIAKGFGVDGEIAESPEQLKAALSRARKATVEGRPYLIDAQVARVGVAWAEKP
                     WIPPISIARERTKKV"
     gene            3194..3979
                     /locus_tag="VEM33_04570"
     CDS             3194..3979
                     /locus_tag="VEM33_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYS51128.1"
                     /translation="MPKADDIETRFRTAGHFTEALKRNLFALYCQPIVPVESAKADYK
                     YLEIFVRFREEEDKLLPPGTFFPILEANHLTPLLDRWVVREVLRWSADKQGSQPNWHI
                     PRFDLNLADDTIRDKDFGGQVRDQLKETKIPPNRLWFEMTGQQMARLPDAAKQTITRL
                     KSLGCGIVVSDFSGVEMTARGYKDAGAQIIKLSGSVVRNIHRNPEALSKLLELNGYCQ
                     KLGLQTIAEFVEHADTLPMLRNAGVNFAQGFAIAEPAPLSLVV"
     gene            complement(3990..4628)
                     /locus_tag="VEM33_04575"
     CDS             complement(3990..4628)
                     /locus_tag="VEM33_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51129.1"
                     /translation="MTRSTGRVSLKSFTAMALAIAAAVPATSNAVDVRPIIKAGFGLG
                     GDTVVSVGVSNSGSTSTRSIKANQGVFFGGGASILTDSKEFEAEVSLSFKFYTIAAQN
                     GRVDWTVLPLDALVFYRMPNVRLGGGLTYHLNPSLSGSGAAGGLNAKYDDALGFVLQG
                     DYVLKNRFNFGLRYTSVEYKRTSFQTNPLIVASTPAANPRTNGIGFVFSMSF"
BASE COUNT          912 a         1522 c         1501 g          775 t
ORIGIN      
        1 tcagccaggg caaccgcccg ccttcgccgg tggagctagg ctgcgcggac ccgaacagtc
       61 cctgccacct gccgaacgcc atgacctccg atccgccgct caagcaggtc gtgtcgcgca
      121 cgctcgaggc cggagcacgc gcgcgaacgg cttcggggct gcggtggaat gcggcgctgt
      181 atcgcaccgt gagctacgac gacatcctgt tcgtgagctc ctcggcgagc tccggcttct
      241 tcaccaactt cggaaagacg cgcaggcagg gtgttgaagc ggctctcgag cgctcggaag
      301 gaaaattctc ctgggcgctc aactacagcc tgatcgacgc gacttaccag tcgtccgccg
      361 tgcttttttc gcaggcgaac agcaccgccg acgcgaacgg cgacatccag gtttcgcccg
      421 gcgaccgcat acccggaatt ccgcgccatc acctgaatgc ctccgcgacc tacgacctga
      481 cggacaggac cacggtcggc gccggcgcgg tcgcagtgtc ccggcagttc gcgcgcggca
      541 acgacaacaa ccggcacgag cccgacggag tgaattacct gggaccgggc gagatcgccg
      601 gctacacgat cttcaacgtg aatctcgact acaagatcga tcgcggcctg cgggcgttcg
      661 ccaaggtctc caacgtcttc gaccggcgct atgcaactgc cggcgcgctg cggcagaatt
      721 tctttccggg cggcagcctg gtggcgccgg gcgcccaggt caacgagacc ttctacgccc
      781 ctggcgcgcc gcgcgccctt tggatcggga tacagctcgt gtccgacgca gcgggagcca
      841 ggcagagccg cgaggcgcag gcgagccgcg attgacactc cctcgaaccc cggcttatcg
      901 ttgccggcaa tcacaggagg cagtcgccat gccctttccc ggtagatcca cccttgcgat
      961 tcttttctgc ggcctgctct ttgcgaccgc ggcgcaggcg cagcgcgcca agccgcaagg
     1021 attccccgat gcgcccggaa aagacgtgct cgtcggcaaa tgctttcagt gccacgcccc
     1081 gaccatgtgg caggaccacc ggcaggaccg caggggctgg gaaggcgtgc tctaccgcat
     1141 ggtcgggcgc ggcgccctct ggacggaaga cgaaataaag accatggccg actacctcgc
     1201 cgcggcatac gggcggcagc cggcggcatc gaaataagaa cgcagtcgag ggagggacta
     1261 tgagagagct ggtcgcacga tatctgtcgc gctcgatttc acggcgcggt ttcctgaagg
     1321 gcctgacgac cgccggaatc tcgctcgcct cggcggaatc catcctggaa tcgctcgtcc
     1381 cgatagccca tgcgcaggag gcgcagcgca tcgctcccga ggcgatcaaa atggtcgagg
     1441 gaacgggggc ggaatgcttc gccgagcagc tgatcgcttc cggcgtcaaa tacgtgttcg
     1501 gcaactcggc tagcgaggat gcgcagttct acgaggcgct ggtcgaccgg cctcagctca
     1561 aatacatcct gacgccgcac gaaggaccgg gggcagcgat ggccgcgggc tacgtgaagg
     1621 cctcgggcga gccgaccatc gtgatgcagg ccgcggtcgt cggcctcgtc aacgccatgg
     1681 ggcagatgtt caacgcgttc aaggagcaga cgccgctcgt tttctacagc taccgcaccg
     1741 accagacgcg ccgcgccggg cgcgacggct tcgaggaagt ggccaatcag gagcagatcg
     1801 tccagccgat gacgaagtat tcgtggctcg cgcggcgcgc cgaaatgatt ccggagaccg
     1861 tccggcgcgg cttcaaggcg gcgtggacgc cgccttgcgg gccgacttac gtttcctggc
     1921 attcggatct gaacgacgag aagacttacg ccgagatcgt cacccaggac aaggtcgacc
     1981 ctcgcatgcg cgtgcgcccc aatccccgcg aagtcgagcg cgccgcaaag ctcctcgtcg
     2041 aggcgaagat gcccctcatg atcgtcggcg acgaggtctg cggcgccaag gccgcatcca
     2101 aggcggtgga gctcgccgag ctcctcggca tgccggtgac ccaggcgcgg cagatttacg
     2161 cgaacttccc cgagtcgcat ccgttgtggg tcggcggccc gcccgcgacg cgcatcgagt
     2221 cgctcgattt cccgaaaaat cccgacgtag tcatgaacgt cgggaacaag ttgcagcaca
     2281 acagtccgca acccatcgtt gcgcgcgaag cgaaattcat cgacatgcgc agcgacagct
     2341 ggagcatggg caacgtgatg aacacggagg tgccgctggt cgccgacgtc gcctacggac
     2401 tggacgacct cagggccgca gtcgaaggaa tcatgacctc cgcgatgcac aggaaggcgc
     2461 gggagcgcgc gcaggaagtg cgccgcttca gcgagaaggc gaaagcgctg cgcgccttgg
     2521 tggcgaagaa ccccgagtgg gaccgcagcc cgatgctcgc cgaccgcgtc acctccgagg
     2581 tcgcccggtt cgcggaccgc gatgccatca tcgtccatga agccgggtcg gtcgtcctgc
     2641 acggttttga tttcgatccg ttgggcggac gcgagctctt cttctattac ggcgcgcacc
     2701 tcggctcggg cgtgggcacc gcggccggcg tgaagctcgc ccgcccgaac cagcaggtga
     2761 tctgcctcgt gggcgacgga tcgttcgttt tcgggccgac cgcgctgtgg aacatggcgc
     2821 gcctggagct gccggtcatc accgtggtct acaacaacca cgcctacagc gggccccaca
     2881 gccgcgtgat cgaaaagacg gcgggaggca agatggtgca gaagcggcaa ttcgtgcacg
     2941 actatctggg cagcccggac atgaacatgg cgcagatcgc caaggggttc ggcgtggacg
     3001 gggaaatcgc cgagtcgccc gagcagctca aggcggccct ctcccgcgcg cgcaaggcca
     3061 cggtcgaggg caggccctat ctcatcgatg cgcaggtggc gcgcgtggga gtcgcgtggg
     3121 cggagaagcc gtggatcccg ccgatcagca tcgcgcggga gcgcacgaag aaggtctaga
     3181 tcgccgcgcc gcgatgccga aagccgacga catcgaaacg cggttccgca ccgccgggca
     3241 cttcaccgag gcgctgaaaa gaaacctgtt cgcgctgtat tgccagccga tcgtgccggt
     3301 ggaatcggcg aaagcggact acaagtatct ggaaatcttc gtgcgctttc gcgaagagga
     3361 ggacaagctg ctgccgccgg gcactttctt tccgatactc gaagcgaacc acctgacgcc
     3421 gctgctggac cgctgggtgg tgcgcgaggt cctcaggtgg agcgccgaca agcagggctc
     3481 ccagcccaat tggcacattc cgcgcttcga cctcaatctc gccgacgaca cgattcgcga
     3541 caaggatttc ggcggacagg tacgcgatca gctcaaggag acgaaaatcc cgcccaaccg
     3601 gctgtggttc gagatgactg gacagcagat ggcgaggctt cccgacgcgg cgaagcagac
     3661 gatcacccgc ctgaagtccc tgggatgcgg catcgtagtc tcggattttt ccggcgtcga
     3721 gatgaccgcc cggggctaca aggacgccgg cgcgcagatc atcaagctct cgggaagcgt
     3781 ggtacggaac atccacagga atccggaagc cctgtcgaaa ctgctggaac tcaacggtta
     3841 ctgccagaag ctcgggcttc agaccatcgc cgagttcgtg gagcacgccg acaccctccc
     3901 gatgctgcgc aacgcgggcg tcaacttcgc ccagggcttc gcgatcgccg agcctgcgcc
     3961 gctcagcctg gtggtttgaa ggtgaagaat cagaagctca tgctgaagac gaaccctatg
     4021 ccgttggtcc tggggttggc cgccggcgta gacgctacga tcaacgggtt cgtttgaaag
     4081 cttgttctct tgtattcgac gctcgtgtag cgcaagccga agttgaacct gtttttcaac
     4141 acatagtccc cctgcagcac gaagccgagc gcatcgtcgt acttggcgtt caagccgccc
     4201 gccgccccgc tgccgctgag gctcggattc aagtggtagg tcaggccgcc gcccaaacga
     4261 acgttaggca tgcggtagaa gacgagggca tccagcggca acacggtcca gtcgacacgg
     4321 ccgttctgcg cggcgatcgt gtagaacttg aacgaaagcg aaacctccgc ctcgaattcc
     4381 tttgagtccg tgagaatcga ggctccgccg ccgaagaaga ccccttggtt ggccttgatc
     4441 gatctcgtgg aggtgctacc gctgttgctc acccccaccg aaaccacggt atcacccccc
     4501 aagccgaagc ctgccttgat gatcggcctg acgtcgaccg cattcgatgt cgcgggaacg
     4561 gcggcggcaa tcgccaaggc catagcggtg aaagacttga gcgatactct ccctgtgctg
     4621 cgtgtcatgg tgttctccct ttagttgata ggcttaaccc cagaacgtga gccagccctt
     4681 gccgacagca tatcgttcgg tccaaatttt
//
LOCUS       DASPZD010000237         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1209515, whole genome shotgun sequence.
ACCESSION   DASPZD010000237 DASPZD010000000
VERSION     DASPZD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209515"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VEM33_04580"
     CDS             complement(<1..1259)
                     /locus_tag="VEM33_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYS51130.1"
                     /translation="MARFLVGVDIGGTFTDCVALDRDGRITATKSSSTPANFAEGMLA
                     AMRVAAERLGLSFERFCSEIAVLTHGTTVGTNALIQRKGARVGLITTRGHEDAIHIMR
                     GSRGVSSRDIARVVHFPSSQKPVPIVPKRLIEGVSERVDCFGEVVVPLNESEAESAIG
                     RLVEAGVEAIGVCFLWSFKYPRHEQRVKEMIRRIAPSLFVSCSTDIAPKWGEYERITA
                     TALNAYIGPVMAKYLGNLDGQLKKSGYAQPLQITQCGGGSISVDRAIESPLLTLDSGP
                     VSGVTGSLYLGKVMEIPNIITTDMGGTSFDVGIIYGGQPAYSFVSNVAQYEYFLPKVD
                     IQAIGSGGGSLARVDAAAKTLAVGPESAGADPGPVCYGKGGTIATVTDADVVLGTINP
                     DNFLGGRIKLDKKKAVDSVQRIASALGL"
     gene            1343..1819
                     /locus_tag="VEM33_04585"
     CDS             1343..1819
                     /locus_tag="VEM33_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HYS51131.1"
                     /translation="MRTRGFTLVEMMVVIGIVAILALMAIPTYQDKFIRDQVAEALPL
                     ADLAKPPVALSWAVLQTFPADNAAAGLPVAEKIVNNFIGSVAIQGGAINITFGNKANR
                     AILGKVLSLRPAVVEDAPIVPVTWVCGYATAPEKMTVKGENKTNIPPGYLPIRCRS"
     gene            1860..>2560
                     /locus_tag="VEM33_04590"
     CDS             1860..>2560
                     /locus_tag="VEM33_04590"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYS51132.1"
                     /translation="MTQQTVGMIGLGIMGSAMSANLMRAGFRVAGFDVLPRRRAEHKR
                     AGGVAAKSPRDLAKRADLIITSLPSARALAEVAAELAASAKRGTVVIETSTLPIKAKE
                     AARDVLAKRGVILLDCPLSGTGAQARVKDLLIYVSGDRSAYRKIVPVLQGFTSANYYV
                     GAFGAGSKMKFVANLLVAIHNVAAAEAMVLGMKAGLDPAMVLKVVAGGAGGSRMFQVR
                     GPMMVKGDYSEATMK"
BASE COUNT          449 a          838 c          836 g          437 t
ORIGIN      
        1 aggccgagcg ccgaggcaat gcgctgcacc gagtccacgg ccttcttttt gtcgagcttg
       61 atacgcccgc cgagaaaatt atccgggttg atcgtgccga gcacgacatc ggcgtcggtg
      121 acggtcgcga tcgtgccgcc cttgccgtag caaacaggac cagggtcggc cccggctgat
      181 tcggggccga ccgcgagcgt cttcgcggcc gcatcgacgc gcgcgagcga tcctccgccc
      241 gagccgatcg cctggatgtc gaccttgggc aggaaatatt cgtactgcgc gacgttggaa
      301 acgaaggagt acgcgggttg cccgccgtag atgattccga cgtcgaacga ggttccgccc
      361 atgtcggtgg tgatgatgtt cggaatctcc atgaccttgc cgaggtagag cgagccggtg
      421 acgccggaca cggggcccga gtcgagcgtg aggagcggcg actcgatcgc ccggtccacc
      481 gagatcgagc cgcccccgca ctgcgtgatc tgcagcggct gcgcgtagcc ggacttcttc
      541 agctgcccgt ccagattgcc caggtacttc gccatcaccg gcccgatgta ggcgttcaac
      601 gcggtcgccg tgatgcgctc gtactcgccc cacttgggcg cgatatcggt cgagcaggag
      661 acgaacagac tcggcgcgat cctgcggatc atctccttca cgcgctgctc gtggcgcgga
      721 tacttgaacg accagagaaa acacacgccg atcgcctcga cgccggcttc caccaggcgc
      781 ccgatcgcgg actcggcctc ggactcgttc aagggcacga ccacctcgcc gaagcagtcg
      841 acgcgctccg acacgccctc gatcaggcgc tttggcacga tgggcacggg cttctggctc
      901 gagggaaagt ggaccaccct cgcgatgtcg cgcgaggaaa ccccgcgcga gccgcgcatg
      961 atgtggatcg cgtcctcgtg gccccgagtc gtgatcaggc cgacgcgcgc gccctttctc
     1021 tggatcagcg cgttcgtgcc gaccgtggtg ccgtgggtga gcaccgcgat ctcgctgcag
     1081 aaccgttcga acgagagccc gagccgctcg gccgccacgc gcatcgcggc gagcatgcct
     1141 tcggcgaagt tggccggggt ggaagaagat ttcgtcgcgg tgatgcggcc gtcgcgatcg
     1201 agcgccacac agtcggtgaa agtgccgccg atgtccacgc cgaccagaaa ccgcgccact
     1261 tttgttcgat tccgctgggt tgagggcggg atagtagcgt attttcatcc gtgccaaagg
     1321 cggggtagac tcgtccccgg ccatgcgcac ccgcggtttc accctcgttg aaatgatggt
     1381 cgtcatcggg atcgtggcca ttctggcgct gatggcgatt cccacttatc aggacaagtt
     1441 catacgggat caggtcgccg aggccttgcc gctcgccgac ctcgccaagc ccccggtcgc
     1501 cctgtcgtgg gccgtcctgc agactttccc ggccgacaac gccgccgccg gcctgcccgt
     1561 cgccgagaag atcgtcaaca acttcatcgg ctcggtggcc atccaaggcg gcgccatcaa
     1621 catcaccttc ggcaacaagg cgaatcgcgc catcctcggc aaggtcctca gccttcgccc
     1681 ggccgtcgtg gaggatgcgc ccatcgttcc cgtgacctgg gtatgcggct acgccaccgc
     1741 gccggagaag atgacggtca agggggaaaa caagaccaat atcccaccgg gctacttgcc
     1801 gatccgttgc cggtcgtagc gccgcaatcg ctagaatgcc aaggcgggga gaaatagaga
     1861 tgacgcagca aaccgtgggg atgatcggcc tcggtatcat gggctcggcg atgtcggcga
     1921 atctcatgcg cgcgggattt cgcgtggccg gcttcgacgt cctaccgcgc cgccgcgccg
     1981 aacacaagcg cgcaggcggc gtcgccgcga agagcccgcg cgatttggcc aaacgggcag
     2041 accttatcat cacttcactt ccttcggcgc gcgccctcgc cgaagtcgcc gccgagctcg
     2101 ctgcatccgc caagcgcggt acggtcgtga tcgagacgag caccctgccg atcaaggcga
     2161 aggaagcggc gcgcgacgtt cttgcgaagc gcggcgtgat cctgctcgac tgccccttga
     2221 gcggcacggg tgcgcaggcc cgcgtcaagg acctgctcat ctacgtgagc ggtgatcggt
     2281 ccgcctaccg caagatcgtc ccggtgctgc agggcttcac cagcgcgaac tattacgtcg
     2341 gcgcgttcgg cgccggctcg aagatgaaat tcgtcgccaa tctgcttgtc gcgatccata
     2401 acgtcgcggc ggcggaggca atggtgctgg gaatgaaggc ggggctcgat ccggcgatgg
     2461 tgctgaaagt cgtcgcgggc ggtgccggcg gctcgcgcat gttccaggtg cgcggaccga
     2521 tgatggtgaa gggcgactac tccgaggcca cgatgaagaa
//
LOCUS       DASPZD010000238         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1288525, whole genome shotgun sequence.
ACCESSION   DASPZD010000238 DASPZD010000000
VERSION     DASPZD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288525"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VEM33_04595"
     CDS             <1..959
                     /locus_tag="VEM33_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS51133.1"
                     /translation="IPGVRGVSPVVAVPFAGSAAWDGRPAAEGQSQEESTANPMLNMD
                     VVSPDYFETLGIPVLRGRGFTEADRANAPSVVVISQSAARHYWPGQDPIGKRLRMGAD
                     LERTVTVIGVVPDTRYRDLREARPSVYFPLRQSFFPFAPLALAIRTSGAPDAQVTVIR
                     RVISETEPGVALTSAAPFETYLDGPLAQPRLNALLLAVFAGAAVVLAAVGLFGAMATM
                     VRQRTRELGVRMALGANAGDLRRMVMGRGLAIAAVGSVLGVIGALLANRLLLGLLYDV
                     TPTDPATLTAVTGFLVVVAAVASLIPARATTRIDPVVALRAE"
     gene            complement(970..1344)
                     /locus_tag="VEM33_04600"
     CDS             complement(970..1344)
                     /locus_tag="VEM33_04600"
                     /inference="COORDINATES: protein motif:HMM:NF008911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYS51134.1"
                     /translation="MLRGYQALRVWQLSMDLVVESYRVSASFPKHEIYGLTSQLRRAA
                     VSIPANIAEGHGRRHMGECLHHLSIANGSLREFETHVRIASRLQYLTDADLQEILRNA
                     DEVARMLHGLAHALRRRRSPGS"
     gene            1456..2811
                     /locus_tag="VEM33_04605"
     CDS             1456..2811
                     /locus_tag="VEM33_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYS51135.1"
                     /translation="MSAPRLLVADDQPDILEALKLLLKGEGFEVETAASPRAVLAAVE
                     ARDFDALLVDMNYTRDTTSGAEGLDLLSRVQGLESPPPIVVMTAWASIDGAVEAMRRG
                     ARDYVEKPWDNRRLLATMRTQVELGRALRTSQRLEIENRRLRREGLPELVAESPAMQP
                     VLRLMERVGPSDANVLILGEHGTGKEVVARWLHASSPRAERPLIAVNLGGIPDGLFES
                     ELFGHVRGAFTDAKTDRIGRFELAEGGTLFLDEIANLPLPQQAKLLRVLETGEYERVG
                     ASRARRADVRVVAATNADIHAEVKAGRFREDLLFRLNTVEIRLPPLRERRADVPLLAA
                     HFLHRYAARYRKAAMEFTPDAMEVLLRHSWPGNVRELDHAIERAMLMAEGGRVQARDF
                     GLDGGGGAAAALDQMSLEDVERVLIQKALTRAAGNVSEAANALGLSRSALYRRLKRHG
                     L"
     gene            2801..>3091
                     /locus_tag="VEM33_04610"
     CDS             2801..>3091
                     /locus_tag="VEM33_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51136.1"
                     /translation="MVSKATPRHERRVFWLALLGGVPAVALALGLLWFGDFTPRTQWT
                     LTLLVAGVWLACAGIVREHVIRPLQTVSNVLAALRERDYTLRARGSNPEDALG"
BASE COUNT          483 a          998 c         1067 g          543 t
ORIGIN      
        1 cgattccagg tgtgcgcggc gtgtcgccgg ttgtggcagt ccccttcgca ggatccgctg
       61 cgtgggacgg acggccggcc gctgaaggac aatcgcagga ggaatccacc gccaacccga
      121 tgctcaacat ggatgtcgtg agtccggact acttcgagac gctcggcatt cccgtcctcc
      181 ggggccgggg cttcacggaa gcggatcgtg cgaacgcgcc gagtgtcgtc gtgatcagtc
      241 aatccgctgc gcgccactac tggccgggcc aggatcccat tggcaagcgc ctgcggatgg
      301 gagccgattt ggagcggaca gtcacggtca tcggtgtggt cccggacacg cgataccgcg
      361 acctgcggga ggcgcgcccc agcgtgtact tcccgctgcg gcaatcgttc ttccccttcg
      421 ccccgctggc gctcgccatt cgtacgagcg gcgctccgga cgcgcaggta acggttatcc
      481 gccgcgtgat cagcgagacc gaacccggtg tcgcgctgac gagcgcggca cccttcgaga
      541 cgtacctcga cggaccgctg gcgcagcccc gtttgaacgc gctgctcttg gctgtgttcg
      601 cgggtgcggc tgtcgtcctt gcagcggtcg ggttgttcgg cgcaatggcc acgatggtgc
      661 gccaacggac ccgtgagctc ggagttcgga tggcattagg ggccaacgca ggagatctgc
      721 gacggatggt gatgggtcgc ggtctggcga tcgccgcggt tggatcggtg ctgggcgtga
      781 tcggtgcgct cttggcaaac cgtctgctct tagggttgct ctatgacgta acgccgacgg
      841 atccggcgac gctcacggcg gtcaccggat tcctcgtagt cgtggcggcg gtcgccagcc
      901 tcatcccggc ccgggcgacg acgcgcatcg atcctgtggt ggcgttgcga gcggaatagg
      961 ggcctggggt caggagcccg gggaccggcg ccgccggagt gcatgggcga gtccgtggag
     1021 catgcgggcg acctcgtcgg cgttgcgcag gatctcctgt agatccgcat cggtgaggta
     1081 ctgcagcctg ctcgcgatcc ggacgtgggt ctcgaattcg cgcaatgatc cgttagcgat
     1141 ggacagatga tggagacact cgcccatgtg gcgtcgcccg tgaccctccg cgatgttcgc
     1201 cggaattgag acagctgccc tacgcagttg gctggtgagt ccatagatct cgtgcttggg
     1261 gaaagacgca ctgacacggt agctctcgac cacgagatcc atcgagagtt gccacactcg
     1321 gagcgcttgg tatccgcgaa gcattcgacc agtcttgagg atacgaccat cggatcatca
     1381 tcatttttcc gcacggcgga cccttcggac gcgacgcact ggccccggac ccctgacccc
     1441 ggtcaccttt agcacatgtc cgcgccacgt ctcctcgtag ctgacgatca accagatatc
     1501 ctcgaagccc tgaagctcct gctcaagggc gaaggcttcg aggtagagac cgccgcttct
     1561 ccccgcgccg tgctggccgc cgtcgaagcc cgcgacttcg acgcgctact tgttgatatg
     1621 aactacaccc gcgatacgac gtccggagcc gaagggctcg acctgctgtc gcgcgttcaa
     1681 ggactggaga gtccaccgcc gatcgtcgtc atgactgcct gggcgagcat cgacggcgcg
     1741 gtcgaggcga tgcgccgcgg cgcgcgggat tacgtcgaga agccgtggga caaccggcgt
     1801 ctgctcgcga ccatgcgcac gcaggtcgag ctgggccgcg cattgcgcac gagtcagcgc
     1861 ctcgagatcg agaaccgcag gctccgcaga gaagggctcc ccgagctcgt cgcggaatcg
     1921 cccgcgatgc agccggtgtt gcggctgatg gaacgcgtgg gcccatcaga cgcgaacgta
     1981 ctgatcctgg gtgagcatgg cacagggaag gaggtcgtgg cgcggtggct gcacgcctcg
     2041 tctccccggg cagagcggcc gctgatcgcg gtgaacctgg gcggcatccc cgacgggctg
     2101 ttcgagagtg agctgtttgg gcacgtgcgt ggcgcgttca ccgacgcgaa gaccgatcgc
     2161 atcgggcgct ttgagctcgc cgagggcggc accctgttcc tcgacgagat cgcgaatctg
     2221 ccgctcccgc aacaggcgaa gctcctgcgc gtgctcgaga cgggcgagta cgagcgcgtg
     2281 ggcgcgtctc gcgcgcgtcg cgccgacgtg cgcgtcgtcg cggccacaaa cgccgacatc
     2341 catgccgagg tgaaggccgg gcgcttccgc gaagatctgc tgtttcgtct caacacggtc
     2401 gagattcggc tgcccccatt gcgcgagcgg cgcgccgatg tcccgctgct cgccgcgcat
     2461 ttcctgcacc gctatgccgc gcgctatcgc aaagcggcga tggagttcac gccggacgcc
     2521 atggaggtgt tgcttcgcca ttcctggccg ggcaacgtgc gggagctcga tcatgcgatc
     2581 gagcgagcca tgctcatggc ggagggcggg cgtgtgcagg cgcgtgactt cgggctggac
     2641 ggcggagggg gcgccgccgc ggcgctcgac cagatgagtc tggaggacgt cgagcgtgtg
     2701 ctgattcaga aggcgctgac acgcgcggcg ggcaatgtca gtgaggcggc caacgctctc
     2761 ggtctctcac gcagcgcgct gtaccgccgg ctcaaacggc atggtctcta aagcgactcc
     2821 gcgtcacgag cggcgcgtct tttggctcgc gctgctcggc ggcgttccgg cggtcgcgct
     2881 cgccctgggc cttctctggt tcggcgactt cactccccgc acacagtgga cgctcacgct
     2941 gctggtcgca ggcgtctggc tcgcgtgcgc gggcattgtg cgggagcacg tgatccggcc
     3001 gctgcagacc gtatcaaacg tgctcgccgc gctgcgcgaa cgcgattaca cgctccgcgc
     3061 ccgcggctcg aatccagaag acgcactcgg g
//
LOCUS       DASPZD010000239         4370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1373355, whole genome shotgun sequence.
ACCESSION   DASPZD010000239 DASPZD010000000
VERSION     DASPZD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373355"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VEM33_04615"
     CDS             complement(<1..205)
                     /locus_tag="VEM33_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51137.1"
                     /translation="MIAPVSNTEPRWPAIIALLAVGGIYAALPSFLTVGPRWLLLVVV
                     VVLLIPTVISYRAGRHRLNRLLGY"
     gene            complement(208..528)
                     /locus_tag="VEM33_04620"
     CDS             complement(208..528)
                     /locus_tag="VEM33_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HYS51138.1"
                     /translation="MKIEQAMWLHEEHELLLEELCELSGLSETELRELVDYGVLAPVD
                     SDARHWSFSADRLVLARSARRLREDFELDPHEVALVLTLLERVHDLEAELRDLRAKLP
                     RRQQ"
     gene            complement(525..1472)
                     /locus_tag="VEM33_04625"
     CDS             complement(525..1472)
                     /locus_tag="VEM33_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HYS51139.1"
                     /translation="MKYKDYYGVMGVDRKASAEEIKNAYRKLARKYHPDVSKEKDAEE
                     KFKELGEAYETLRDPEKRAAYDQLGSHRPGQEFRPPPGWERQFGDGSFSFDDIDLGDL
                     FAGIAGGMHRAGRRGGKVPIPGEDYEVTAHISLEDAYRGTEVDLELEVPEYDERGFPR
                     RVRRAFKARIPKGAADGQRLRLPGKGGKGLNAGRDGDLYLNIALHPHRLFRVSGHDLF
                     LDLPLAPWEAVLGTSVQVPTLAGSVRLKVPPGTRAGQQLRLAGRGLPKPHGGEGDLFA
                     IVQVAVPTVTSERERALFKELAEASTFNPRGHFEQEAAK"
     gene            complement(1509..1703)
                     /locus_tag="VEM33_04630"
     CDS             complement(1509..1703)
                     /locus_tag="VEM33_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51140.1"
                     /translation="MNIIWRFYSDPAHAWRWQQISADRTVVSESHRAYKNYEGCLADA
                     REKGYVFQAPQPKSNKGRLR"
     gene            complement(1738..1944)
                     /locus_tag="VEM33_04635"
     CDS             complement(1738..1944)
                     /locus_tag="VEM33_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYS51141.1"
                     /translation="MPTGKVKWFNASKGFGFIQADDGSKDVFVHVSAVEKAGLSTLNE
                     GQKVGYELVTGKNGKTSAENLKAL"
     gene            2127..3212
                     /locus_tag="VEM33_04640"
     CDS             2127..3212
                     /locus_tag="VEM33_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HYS51142.1"
                     /translation="MKRLLFGLMLLASPVSAQDSVPQIPFDGTDPLKLPKDLYLGEVT
                     GVAVNSKGHVFVLSRGNTTGPAYAAAAAQLLEFDPEGNFVREIGKNLYAWSFGHTVRV
                     DPSDNIWVTDKGSDMVVKFNSRGRVSMVFGRKLEASDRETAPLERPNPPLPHEDGRFR
                     QVTDVAFDPAGNAFISDGYINSRVAKVDRNGKWLKSWGSKGSKPGEFNTPHSIAADAK
                     GNIYVADRGNRRIQVFDGEGAFQREFTIDVPYDAKAARPAIGNLPDLAKIEAGGGPKT
                     MMPGAPWTVCITPGPNPVLYASDAFPGRVFKLSLDGKVLGVLGESGKQLKQFGWIHEI
                     ACPSENVLYVAELLNWRVQKLVLHPTK"
     gene            complement(3279..3473)
                     /locus_tag="VEM33_04645"
     CDS             complement(3279..3473)
                     /locus_tag="VEM33_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51143.1"
                     /translation="MNIIWRFYSDPAHAWRWQRISADRTVVSESQRSYKNYEGCLADA
                     RDKGYVFHAPQPKSNKGRLR"
     gene            complement(3489..3695)
                     /locus_tag="VEM33_04650"
     CDS             complement(3489..3695)
                     /locus_tag="VEM33_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYS51144.1"
                     /translation="MPTGKVKWFNASKGFGFIQADDGSKDVFVHISAVEKAGLPALNE
                     GQKVGYELVVGKNGKTSAENLKAM"
     gene            3900..>4370
                     /locus_tag="VEM33_04655"
     CDS             3900..>4370
                     /locus_tag="VEM33_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51145.1"
                     /translation="MRRLFSLSRAIRVTAFLLLLGSGAAIAEDAAPPEYPKFGLLRER
                     DLTPFGFLRLDMRPAHAVWAPPGNWAVEVLLGYQNTWAMSPNVEQYLKSLPGRRRLGP
                     ADLAAIRALPGEAYLVDLELGLVDVALHKRLTEHWSVNATFAAVNYSGGFMDGLI"
BASE COUNT          751 a         1447 c         1365 g          807 t
ORIGIN      
        1 tgtaacccag aagacgattg agtctatggc gtcctgcacg ataagagatc accgtcggaa
       61 tcaacagaac gacaacgacc acgagcagca accaacgcgg ccctacggta aggaaagagg
      121 gaagcgcggc ataaatgcct cccacggcta gaagcgcgat gattgccggc cagcgcggtt
      181 cagtattgga aacgggggcg atcaagatta ctgctgcctg cgtggcagct tcgcgcgcag
      241 gtcgcgcagc tccgcctcga gatcgtggac gcgctcgagc agcgtgagca ccagggccac
      301 ctcgtgcggg tccagctcga aatcctcgcg cagacggcgc gcgctgcgcg caaggaccag
      361 ccggtccgcg ctgaagctcc agtgccgtgc gtccgaatcg accggcgcga gcactccgta
      421 atccacgagc tcgcgcagct ccgtttcgga cagccccgac agctcgcaga gctcttcgag
      481 caggagctcg tgctcctcgt gcagccacat cgcctgttcg attttcactt ggcagcctcc
      541 tgctcgaaat gtccgcgcgg gttgaaggtc gaggcctcgg cgagctcctt gaacagcgcg
      601 cgctcgcgct cgctcgtcac ggtcggcacc gcgacctgga cgatcgcgaa gaggtcgccc
      661 tctcccccgt gcggcttggg gaggccgcgc ccggcgagcc gcagctgctg gccggcgcgc
      721 gtccccggcg ggaccttgag gcgcaccgat cctgcgaggg tgggaacctg cacactcgtg
      781 ccgagcacgg cttcccacgg cgcgagcgga agatcgagga agagatcgtg gccgctcacg
      841 cgaaagagcc ggtgcggatg caacgcgata ttgagataca agtcgccgtc gcgtccggcg
      901 ttcagcccct tgcccccctt gccgggcagg cgcaggcgct ggccgtccgc ggcgcctttc
      961 ggaatgcgcg ctttgaatgc gcgccgtacg cggcgcggga aaccgcgctc gtcgtactcg
     1021 gggacttcga gctcgagatc gacctcggtg ccgcgataag cgtcctccaa tgaaatgtgc
     1081 gcggtgactt cgtagtcttc tccgggaatt ggaaccttgc cgccgcgccg tccggcgcgg
     1141 tgcatcccgc ccgcaatgcc ggcgaagaga tcccccaggt cgatatcgtc gaacgagaac
     1201 gagccgtcgc cgaattgccg ctcccacccc gggggtggcc ggaactcctg tccggggcga
     1261 tggctgccca gctggtcgta ggcggcgcgc ttttccgggt ccctcagggt ttcataggct
     1321 tcgccgagct ccttgaactt ctcctcggcg tccttttcct tggaaacgtc cggatgatat
     1381 tttcgcgcga gcttgcggta cgcgttcttg atctcctcgg cgctcgcctt gcggtcgacg
     1441 cccatcaccc cgtagtagtc cttgtatttc atccggactc cgggccgctt ccgccggacg
     1501 gcattgctct agcgcaggcg ccccttgttc gacttgggtt gcggcgcctg aaaaacgtaa
     1561 cccttttctc tggcatcggc caggcatccc tcgtaattct tgtaggcccg atgggattcc
     1621 gagacgaccg tccggtccgc ggaaatctgc tgccacctcc aggcgtgcgc ggggtcgctg
     1681 tagaagcgcc agatgatgtt catctgcaga aattctcccc ggggcgatcg cgagcggtca
     1741 caacgccttc aggttctcgg ccgaagtctt gccgttcttg cccgtgacca gctcgtatcc
     1801 caccttctgg ccttcgttca aggtggacag gccggccttc tccaccgccg agacatgcac
     1861 gaaaacgtcc ttcgaaccat cgtctgcctg aatgaaacca aaacccttcg atgcgttgaa
     1921 ccacttcact tttccggttg gcacaagact ctcctgacga aagaaattgg attgaaagcc
     1981 cgcccggacc cgcgcttcgg cccggagcga cggaagctag tttagtccaa ttcgccgccg
     2041 ctgcgcgcgt gtctgccgtt atactcgccc gttgcaggag gaacccccgg cgcaccgcgc
     2101 cgcaacaccc gctcaaggag gatgtcatga aacgcctgct tttcggcctg atgctgctcg
     2161 cgtcgcccgt atcggcgcag gactcggtgc cgcagattcc gttcgacggg accgacccgc
     2221 tcaagctgcc gaaagacctg tatctcggcg aagtcaccgg cgtcgcggtg aactcgaagg
     2281 ggcacgtctt cgtcctgtcg cgcggcaata ccacgggccc ggcctacgcg gctgcggcgg
     2341 cgcagctgct cgagttcgat cccgaaggaa atttcgtgcg ggagatcggc aagaacctgt
     2401 acgcctggtc gttcggccat accgtccgcg tggacccgag cgacaacatc tgggtcacgg
     2461 acaagggatc ggacatggtg gtcaagttca actcccgggg ccgggtctcg atggtcttcg
     2521 gacgcaagct ggaagcatcg gacagggaaa cggcgccgct cgaacgcccc aatccgccgc
     2581 tgccccacga ggacgggcgc ttcaggcagg tgaccgacgt cgccttcgat ccggccggca
     2641 atgcgttcat cagcgacggt tacatcaatt cgcgcgtcgc caaggtggac aggaacggca
     2701 aatggctcaa gtcgtgggga agcaagggct cgaagcccgg cgagttcaac acgccgcact
     2761 cgatcgccgc cgatgcgaag ggcaacatct acgttgccga ccgcggcaac cggcgcatcc
     2821 aggtgttcga cggggagggc gccttccagc gcgagttcac gatcgacgtg ccgtacgacg
     2881 cgaaggcggc gcgcccggcg atcggcaacc tgcccgacct ggcgaagatc gaagcggggg
     2941 gcgggccgaa gacgatgatg cccggcgcgc cgtggacggt gtgcatcacg cccgggccga
     3001 acccggtgct ctacgcttcc gacgcattcc cgggccgcgt gttcaagcta tcgctggacg
     3061 gaaaggtgct cggagtgctc ggcgaatccg gcaagcagct gaagcagttc ggctggatcc
     3121 acgagatcgc ctgcccgtcg gagaacgtgc tctacgtcgc ggagctgctc aactggcgcg
     3181 tgcagaagct ggttctgcac ccgacgaaat aacgcgggca gcccgtgaga ccggggcggc
     3241 gctcgccccg gatgcggttc cgatgagatc gagtgtctct agcgcaagcg ccccttgttc
     3301 gacttgggct ggggcgcatg gaatacgtag cccttgtccc ttgcgtcggc caggcatccc
     3361 tcgtagttct tgtaggaccg ttgcgattcc gagaccaccg tgcggtccgc ggaaatccgc
     3421 tgccaccgcc aggcatgcgc ggggtcgctg tagaagcgcc agatgatatt cacccgcgaa
     3481 atcctggatc acatcgcctt gaggttttcg gcggacgtct tgccgttctt tcccaccacc
     3541 agttcgtatc ctaccttctg gccttcgttc agggcgggca gcccggcctt ctccaccgcc
     3601 gagatgtgca cgaagacgtc tttcgagccg tcgtccgcct gaatgaaacc gaagcccttc
     3661 gatgcgttga accacttcac tttaccggtt ggcacgggtg tctctcctat gagcgctttt
     3721 tggataccgc cccggccatc gtttcggcgc ggggcgatag ggggtagtct actccgttcc
     3781 tttcgcggcg ccatccatcc atcggctgga acgatggtct tcatcgacaa atatgggcta
     3841 ccgcgctgag cgacgcaacg atatcttcag cgcatccttt ccaacagggc gcgacgttga
     3901 tgcgtaggct tttctcgctg tcacgcgcga tccgcgtgac ggcgttcctg ctattgctcg
     3961 ggtccggggc cgcgatcgcg gaggacgctg cgccgcccga gtacccgaaa ttcggcctgc
     4021 tgcgcgaacg cgatctcacg ccgttcggct ttctgcgcct ggacatgcgt cccgcgcacg
     4081 ccgtctgggc tccgccgggc aactgggccg tcgaagtcct tctcggctac cagaacacct
     4141 gggcgatgag tccgaacgtt gagcaatacc tcaagtcgct gcccggccgc cgccgcctcg
     4201 gccccgcgga cctggcggcg atccgcgcgc tgcccggcga agcctatctc gtcgatctcg
     4261 agctcgggct ggtcgacgtg gccttgcata agaggctgac cgaacattgg agcgtcaacg
     4321 ccactttcgc cgccgtgaac tacagcggcg gcttcatgga cggcttgatc
//
LOCUS       DASPZD010000240         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_756269, whole genome shotgun sequence.
ACCESSION   DASPZD010000240 DASPZD010000000
VERSION     DASPZD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756269"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VEM33_04660"
     CDS             <1..298
                     /locus_tag="VEM33_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYS51146.1"
                     /translation="VEKPRPEAAPSNLAVVGRYILTPRIFHHLAQARPGAGSEIQLTD
                     AIASLLADDRVLAYSFKGTRYDCGSKLGYLQATVAYGLKHPEVGKGFERFLKSL"
     gene            336..881
                     /locus_tag="VEM33_04665"
     CDS             336..881
                     /locus_tag="VEM33_04665"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HYS51147.1"
                     /translation="MTPDEAWKILEGADRLCDEATVHAAITRLAGEITARLKDRYPLV
                     LAVMGGSIFFAGHLLPRLRFPLEFDYIHASRYGNATSGGGIVWTVAPRENVRDRTVLV
                     LDDILDGGETLAAIRDRVNSLGAAAFHSAVLTDKDIGRDKPIVPDFVGLKLPNRYVFG
                     CGMDVSGAWRNLPEIYAVKDS"
     gene            888..1622
                     /locus_tag="VEM33_04670"
     CDS             888..1622
                     /locus_tag="VEM33_04670"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HYS51148.1"
                     /translation="MLAIIGGTGLTQLSNLDVSRRQVVRTPYGEPSGPLTFGVIRGVT
                     VVFLARHGYGHTIPPHQVNYRANLWALKEQGAGDIVSVVSVGGIRADLGPGKIVVPHQ
                     IIDYTCGRMNTFFENEAPVKHIDFTEPYTGELRSRLLTAAKACGEAAVDGAVYAATQG
                     PRLETAAEIDRLERDGADIVGMTGMPEAALAREIELAYAAIAVVVNHAAGRGASARGV
                     RLQEIDEVLRDAVGRVRKIIEALVAA"
     gene            1619..2149
                     /gene="def"
                     /locus_tag="VEM33_04675"
     CDS             1619..2149
                     /gene="def"
                     /locus_tag="VEM33_04675"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYS51149.1"
                     /translation="MIREVLKMGDLRLLEKSQPVEAFGTPQLRELIADMRDTMAHLNG
                     AGLAAPQIGVPLRVVIFGVTLNPRYPDAEEVPDTVLINPVITPLEEEMEEDWEGCLSV
                     PGMRGVVPRFKKVRYQGFDESGNPIDRSVEGFHARVVQHECDHLDGILYPMRMRDMSQ
                     FGFVDVLFPDQELRDE"
     gene            complement(2166..>2501)
                     /locus_tag="VEM33_04680"
     CDS             complement(2166..>2501)
                     /locus_tag="VEM33_04680"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656590.1"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HYS51150.1"
                     /translation="AGWLAKQVPLPQPVQGRVSRRVKHFPIAPEVHIVPDEKGQYHSL
                     SITAGDRPGLLYAIALVLSRYRVSLHTAKIATLGERAEDVFVVSGAALANPKTVLQLE
                     TDLLNALQA"
BASE COUNT          446 a          789 c          837 g          429 t
ORIGIN      
        1 cgtcgagaag cccaggccgg aagccgcccc gtcgaacctt gccgtggtcg ggcggtacat
       61 tctgacgccg cgcatttttc accacctggc gcaggccagg cccggggcgg gaagcgaaat
      121 tcagctcacc gacgcgatcg catcgctgct cgccgacgat cgcgtcctcg cgtacagctt
      181 caagggaacc cgctacgact gcggctcgaa actcgggtac ctgcaggcga cggtggcgta
      241 cggactcaag catcccgagg tcggaaaggg attcgagagg tttctgaaat cgctgtgaag
      301 gggccagagg gccggggcgc tatacttctc gcgccatgac gcccgacgag gcctggaaga
      361 tcctggaagg cgccgaccgg ttgtgcgacg aagccacggt gcatgccgcg atcacgcgcc
      421 ttgcggggga gatcacggcg agactgaaag accgctatcc tctcgtgctc gccgtgatgg
      481 gcggctcgat attctttgcc ggacatctcc tgcctcggct gcgcttcccg ctcgagttcg
      541 actacatcca cgcatcgcgc tacggcaacg cgacctcggg cggcgggatc gtatggacgg
      601 tggcgccgcg ggagaacgta agggaccgca ccgtgctcgt gctggatgac atcctcgacg
      661 gaggagaaac gctcgcggcg atccgtgacc gtgtgaactc gctcggtgcg gcggcgttcc
      721 acagtgccgt gctcaccgac aaggacatcg gccgcgacaa gcccatcgtc cccgatttcg
      781 tcggtttgaa gctgcccaac cgctacgtgt tcggctgcgg gatggacgta tccggcgcct
      841 ggcgcaacct tccggaaatc tacgcggtaa aggattcctg agcgcacatg ctcgcaatca
      901 tcggcggcac ggggctgaca cagctctcca atctcgatgt ttcgcgtcgc caagtcgtgc
      961 ggactcctta tggggagccg tccgggccgc tcactttcgg cgtgatccgc ggcgtgacgg
     1021 tggtgttcct cgcgcgccac ggctacggcc acacgattcc tccccaccaa gtgaactacc
     1081 gcgccaactt gtgggcgctc aaggagcagg gcgccggcga catcgtttcg gtcgtctcgg
     1141 tcggcggcat ccgtgcagac ctcggcccgg ggaagatcgt cgtcccgcac cagatcatcg
     1201 actacacctg cggtcggatg aacacgtttt tcgagaacga ggcccctgtg aagcacatcg
     1261 acttcaccga gccgtacacc ggcgagctgc gcagcaggct tcttacggcg gcgaaggctt
     1321 gcggagaggc cgccgtcgac ggcgccgtct atgctgcgac ccagggtccg agacttgaaa
     1381 cggccgcgga gatcgaccgc ctggagcgcg acggcgccga catcgtcggc atgaccggga
     1441 tgcccgaggc ggccctggcg cgcgagatcg agctggccta cgcggcgatc gcagtggtcg
     1501 tcaatcatgc cgctggccgg ggcgcaagcg cccgcggcgt acggctgcaa gagatcgacg
     1561 aagtactgag ggacgccgtg ggccgggtgc gaaagatcat cgaagcgctg gtggcggcat
     1621 gatccgcgaa gtcttgaaaa tgggtgatct gcggctgctc gagaaatcgc aaccggtgga
     1681 agcgttcggc acgccgcagc tgcgcgagct gatcgccgac atgcgcgaca ccatggcgca
     1741 cctgaacggc gccgggctcg ccgctccgca gatcggcgtg ccgctgcgcg tggtgatttt
     1801 cggtgtcacg ctcaaccctc gctatcctga tgcggaagaa gtccccgaca cggtgctcat
     1861 caatcccgtc atcaccccgc tcgaggaaga aatggaagag gactgggagg gttgcctgtc
     1921 ggtcccgggg atgcgcggcg tcgtgccgcg cttcaagaag gtccggtatc agggcttcga
     1981 cgaatcgggc aatccgatcg atcggagcgt ggaagggttt catgcgcggg tcgtacagca
     2041 tgaatgcgac catctcgacg gaatcctgta cccgatgcgc atgcgtgaca tgtcccaatt
     2101 cggcttcgtc gacgttctgt tccccgatca ggagctcagg gacgaatgag accgcggagc
     2161 tgcgtctagg cttgcagggc gttcagcaga tcggtttcga gctggagcac ggttttcgga
     2221 ttggcgagcg ccgcgccgga cactacgaac acgtcctccg cgcgctcgcc gagcgtagcg
     2281 atcttggccg tgtgcaggct gaccctgtaa cggctgagca ccagcgcgat ggcatagagc
     2341 aggccgggcc ggtcgccggc cgtgatcgac agggaatgat actgcccctt ctcgtccggg
     2401 acgatgtgca cctcaggtgc gatggggaag tgcttgacgc gccgcgacac ccgcccttgc
     2461 actggctgcg gcaagggaac ctgtttcgcg agccagccgg c
//
LOCUS       DASPZD010000241         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_16290, whole genome shotgun sequence.
ACCESSION   DASPZD010000241 DASPZD010000000
VERSION     DASPZD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16290"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VEM33_04685"
     CDS             <1..1202
                     /locus_tag="VEM33_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HYS51151.1"
                     /translation="ELPRAAAIVNPNQTGCAFPSKSLAGVGVMFYVMLALRAELRKRG
                     AFTGKEEPNLGDVLDLVALGTVAEVVPLDFNNRVLVAQGLKRIRAGRMQEGVRALFAV
                     AGRDPSRASGFDLGFLLGPRLNAAGRLSDMSLGIECLTTGDRGRALEIARQLDELNRA
                     RRTIEADMQAQAHELMAKLSVGETSSISLYDPSWHQGVVGILAGRVKDRHHRPTFAFA
                     PAGAGEIRGSGRSILGLHLRDALDLVAKREPDLLLRFGGHAAAAGLTLRPADFARFAE
                     SFEAVARELLSPAQLARAVETDGSLEPAYLELGLAQLLEHQVWGQGFPQPLFCDEFDV
                     LGQRVVGEKHLKLRLARAGRSVEAMRFNALDPLPSPVRAAYRLSVNEFNGMQNLQLVI
                     EHWEPTR"
     gene            1199..1576
                     /locus_tag="VEM33_04690"
     CDS             1199..1576
                     /locus_tag="VEM33_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HYS51152.1"
                     /translation="MSYARITISWPKGALEATLEDTPTARALVAALPMEAKAQTWGEE
                     VYFEIPVTAKLEINANQVVPPGTVCFWTEGSSLALPWGRTPVSEGDESRLVTRCNVLG
                     RIDGEPRQLASVRSGDSITVSLE"
     gene            1579..1791
                     /locus_tag="VEM33_04695"
     CDS             1579..1791
                     /locus_tag="VEM33_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HYS51153.1"
                     /translation="MERKRINSSKIRAVGYDPKSQMLEVEFSDGRLIQYRGVSPEVHR
                     QFMAAPSATSFFEDKIDESFPSNRIK"
     gene            1832..2936
                     /gene="prfB"
                     /locus_tag="VEM33_04700"
     CDS             join(1832..1912,1914..2936)
                     /gene="prfB"
                     /locus_tag="VEM33_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095522732.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HYS51154.1"
                     /translation="MEAERINQIASTLSDLDKRSAELRRYLDFEGKEKRLTEVETLLT
                     DPKVWNDPKRAQDLGREKKSLDGTVTRLAAIASSLRDSRGLFEMARAENDDATLSGIA
                     EDIAKLDKEVGDLEFRRMFSDPLDPNNCFMDIQSGSGGTEAQDWAQMLERMYAKYCDR
                     KGYGMEVLEESPGEVAGIKSASLKISGSYAYGHLRTENGVHRLVRKSPFDSNARRHTS
                     FASVFVYPEVDENVQVEVNPADLRIDTYRASGAGGQHVNKTDSAVRITHLPTGIVVQC
                     QNDRSQHRNRAEAMAMLKSRLYELELRKLQADRDKLESAKTDIEWGHQIRSYVLDQSR
                     IKDLRTGVEKGDTQRVLDGDLDDFISASLKQGV"
     gene            2945..>3180
                     /locus_tag="VEM33_04705"
     CDS             2945..>3180
                     /locus_tag="VEM33_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HYS51155.1"
                     /translation="MSDQPVPPDENQIIAERRAKLAELRRRGVAFPNDFPREHLAADL
                     HKTWDGKSNDEIEPKAVKATIAGRMMLKRVMGKA"
BASE COUNT          597 a         1042 c         1046 g          495 t
ORIGIN      
        1 cggagctgcc gcgcgccgcg gccatcgtca atcccaacca gacaggctgc gcgtttccga
       61 gcaagagcct tgcgggggtc ggcgtgatgt tctacgtgat gctcgccctg cgggccgagc
      121 tccgcaagcg cggggcgttc accggcaagg aggagccgaa tctaggcgac gtgctcgatc
      181 tggtggcgct cggcacggtc gccgaagtcg tgccgctcga cttcaacaac cgcgtcctcg
      241 ttgcgcaggg cttgaagcgc atccgcgcgg gccgcatgca ggaaggcgtg cgcgcgcttt
      301 ttgccgtcgc cgggcgcgac ccgtcgcggg cgagtggctt cgatctcgga tttctgctcg
      361 ggccgcggct gaacgcagcc ggacgcctgt ccgacatgag cctggggatc gagtgcctga
      421 cgaccggaga ccgcgggcgc gccctggaga tcgcgcggca gctcgacgag ctcaaccgcg
      481 cgcgccgtac gatcgaagcc gacatgcagg cgcaggccca cgagctgatg gcgaagctct
      541 cggtgggcga gacctccagc atcagcctgt acgacccttc ctggcaccag ggagtggtcg
      601 ggattctcgc cgggcgcgtc aaggaccgtc accaccgccc gaccttcgcc ttcgctccgg
      661 ccggcgccgg ggagatccgc ggctccggcc ggtcgattct cggcctgcat ctgcgcgacg
      721 ccctggatct cgtcgcgaag cgcgaaccgg acctgctcct gcgcttcggc gggcacgccg
      781 cggccgcggg cctgacgctg cgcccggcgg atttcgcgcg attcgccgag agcttcgagg
      841 cggtggctcg cgagctgctc tcgcccgcgc agcttgcgcg cgcggtcgag accgacggtt
      901 cgctggaacc ggcatacctc gagctcggcc tcgcgcagct gctcgagcat caagtctggg
      961 gacagggttt tccccagccg ctcttctgcg acgagttcga cgtgctcggc cagcgcgtgg
     1021 tcggcgagaa gcacctgaag ctcaggctcg cgcgcgccgg gcgcagtgtc gaagcgatgc
     1081 gcttcaacgc gctcgatccc ctgccctcgc ccgtgcgcgc ggcgtaccgg ctttcggtca
     1141 acgagttcaa cggaatgcag aatctgcaat tggtcatcga gcattgggag cccacgcgat
     1201 gagttacgcg cgcataacga tttcctggcc gaagggtgcg ctggaagcga ccttggagga
     1261 cacgcccacc gcgcgcgccc tggtcgccgc gctgcctatg gaagcgaaag cccagacctg
     1321 gggcgaggag gtctatttcg agatcccggt cacggcgaaa ctcgagatca acgcaaatca
     1381 ggtcgtgccg ccgggaacgg tctgtttctg gaccgaaggc agctcgctcg ccctgccctg
     1441 gggccggact cccgtatccg aaggcgacga gtcgcgcctg gtgacccgct gcaacgtgct
     1501 tggccggatc gacggcgagc cccggcagct cgcctcggtg cgcagcggcg acagcatcac
     1561 cgtatcgctg gagtgaccat ggaacgaaaa cgcatcaact cgagcaaaat tcgcgcagtc
     1621 ggctacgacc cgaagtcgca gatgctggaa gtcgaattca gcgacggcag gctgatccaa
     1681 taccgagggg tctcccccga ggtccatcgc cagttcatgg cggcgccttc ggcgacgagc
     1741 tttttcgagg acaagatcga cgaaagtttt ccttcgaatc ggataaagtg agtcgcagtg
     1801 ccctataatg cgcgttttgc aaagaaaccg aatggaagcc gaacgcatca atcaaatcgc
     1861 ctcgacgctc tcggacctcg ataagaggtc ggcggagctg cggaggtatc tttgacttcg
     1921 agggcaaaga gaaacgcctg acggaagtcg aaacgctcct gaccgatccc aaggtctgga
     1981 acgacccgaa acgcgcgcag gacctcggca gggaaaagaa gtctctggac gggaccgtca
     2041 cccggctcgc cgcgatcgcc tcgtcgctgc gcgattcgcg cggcctcttc gagatggcgc
     2101 gcgcagagaa cgacgacgcg actctatccg ggatcgccga ggacatcgcg aagctggaca
     2161 aggaggtcgg cgacctggag ttccgccgca tgttttccga tcccctggat ccgaacaact
     2221 gcttcatgga catccagtcg ggatcgggcg gcaccgaggc gcaggactgg gcgcagatgc
     2281 tcgagcgcat gtacgcgaag tactgcgacc gcaagggcta cggcatggag gtgctcgagg
     2341 agtcgccggg cgaggtcgcc ggcatcaaga gcgcctcgct caagatcagc ggcagctacg
     2401 cctacgggca cctgcgcacc gagaacggcg ttcaccgcct ggtgcgcaag tcccctttcg
     2461 attcgaacgc gcgccgccac acctccttcg cgagcgtgtt cgtctacccc gaggtggacg
     2521 agaacgtcca ggtggaagtg aatcccgccg atttgcgcat cgacacctac cgcgcatccg
     2581 gcgcgggcgg ccagcacgtc aacaagaccg attcggcggt gcgcatcacg catttgccga
     2641 cgggcatcgt ggtacagtgc cagaatgatc gctcgcagca ccgcaaccgc gcggaagcca
     2701 tggcgatgct gaaatcgcgc ctgtacgagc tggaactgcg caagctccag gccgaccgcg
     2761 acaagctcga gtccgccaag accgacatcg agtggggaca ccagatccgc agctacgtgc
     2821 tcgaccagtc gcgcatcaag gacctgcgta ccggcgtgga gaagggcgac acgcagaggg
     2881 tgctcgacgg cgatctcgac gatttcatct ccgcgagctt gaagcagggc gtctaaccgc
     2941 gatcatgtcc gatcagccgg ttccgccgga cgaaaaccag atcatcgccg agcggcgcgc
     3001 caagctcgcc gagctgcgcc ggcgcggggt cgcatttccg aacgatttcc cgcgcgagca
     3061 cctcgcggcc gaccttcaca agacatggga cggaaaatcc aacgacgaga tcgagccgaa
     3121 agcggtgaaa gcgaccatcg ccggccgcat gatgctcaaa cgcgtcatgg gcaaggcgag
//
LOCUS       DASPZD010000242         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_89669, whole genome shotgun sequence.
ACCESSION   DASPZD010000242 DASPZD010000000
VERSION     DASPZD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89669"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2256)
                     /locus_tag="VEM33_04710"
     CDS             complement(<1..2256)
                     /locus_tag="VEM33_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HYS51156.1"
                     /translation="MNAPAPHHEITLDDKYALQSGRAFMTGTQALVRLPMLQRVRDLA
                     AGLSTAGFISGYRGSPVGGFDQALWKAKKFLDAHHVKFQPGVNEDLAATSIWGTQQVN
                     LYPDAKYDGVFAMWYGKGPGVDRCGDVFKHANSAGTSRHGGVLVLAGDDHAAKSSTLA
                     HQSEHIFKACCIPVLNASSVQEYLDLGLHGIAMSRFSGCWVAFKCVTDVIESGAVVDI
                     DPQRVNIVMPDFAMPPGGLNIRWPDAFLEQEARLLDYKVYAALAYCRANRLDRIVWDS
                     PKARFGIVTTGKSFGDTLQALADLGIDERVAADAGIRLYKVAMSWPLEPQGARRFAEG
                     LEEILVVEEKRQVIEYQIKEELYNWREGMRAPRVVGKFDDNGEWSIAEGKPAGNWLLP
                     AHYELSAALIAKALASRLAKLGLDRAMGERYRERLAYLEFKEKALAKPRVVESRLPYF
                     CSGCPHNTSTKVPEGSRAMAGIGCHFMAVWMDRNTSTFTHMGAEGAPWIGQAPFTECP
                     HVFANLGDGTYFHSGLLAIRAAVAAGVSMTYKILYNDAVAMTGGQPFDGPLDPAMISR
                     QIAAEGVSPIVAVVDEKEPLLAGLAWAPGTTIRPREHLDAVQRELREKKGVSAIIYVQ
                     TCATEKRRRRKRKEFPDPSRRVVINEMVCEGCGDCSVQSNCLSVEPLETEFGRKRTIN
                     QSSCNKDYSCLKGFCPSFVTVEGGNLKKGKAALPEGEAEFAVLAPRSALDRPFGILVT
                     GIGGMGVITIGQ"
     gene            complement(2307..>2791)
                     /locus_tag="VEM33_04715"
     CDS             complement(2307..>2791)
                     /locus_tag="VEM33_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS51157.1"
                     /translation="LALLDTTARADTAEQTRKRRDFIALAERGRFLGITDVLLPLLIH
                     PSQLANRELASTIKKMAKNTGRDAFIRQERAIISRSDSLPLLSRIACPTLVLCGRQDA
                     LIPLALHEEMASGIPESKLEIVEECGHLSTLERPVETSAALAHWLEPSRSSVRSNQGA
                     "
BASE COUNT          443 a          903 c          934 g          511 t
ORIGIN      
        1 ctggccgatg gtgatcacgc ccatgccgcc gattcccgtc acgaggatgc cgaacgggcg
       61 atcgagagcg ctccgcggcg ccaggaccgc aaattcagct tccccttccg gcagcgccgc
      121 cttgcccttt ttcaggttgc cgccttcgac cgtgacgaag ctcgggcaga atcccttcag
      181 gcaggaatag tccttgttgc aggaggactg gttgatggtg cgcttgcgac cgaactcggt
      241 ctcgagaggt tcgaccgaaa ggcaattcga ctggacgctg caatcgccgc agccttcgca
      301 gaccatctcg ttgatgacca cgcgcctgga gggatcgggg aactccttgc gctttctgcg
      361 ccggcgcttc tcggtggcgc aggtctgcac gtagatgatc gccgaaacgc cttttttctc
      421 gcgcagctcg cgctgcaccg cgtcgaggtg ctcgcgcggc cggatcgtcg ttccgggggc
      481 ccaagccagt cccgccagca atggttcctt ctcgtccacg accgcgacga tcgggctcac
      541 gccttcggcg gcgatctggc gcgagatcat cgccgggtcg agcgggccgt cgaagggctg
      601 gccgccggtc atcgccaccg cgtcgttgta caggatcttg taggtcatgc tcacgccggc
      661 cgccaccgcg gcgcggatcg cgagcagccc cgagtgaaag taggtgccgt cgccgaggtt
      721 ggcgaacacg tgcgggcact cggtgaatgg tgcctgcccg atccagggcg cgccttcggc
      781 tcccatgtgc gtgaacgtcg aagtgttgcg atccatccag accgccatga agtggcagcc
      841 gatgccggcc atggcgcggc tgccctcggg aaccttggtc gaagtgttgt gcgggcaacc
      901 cgagcagaag tacggcagcc ggctctcgac cacgcgcggc ttcgccagcg ccttctcctt
      961 gaactcgagg taggcgaggc gctctcggta gcgttcgccc atggcgcggt cgagcccgag
     1021 cttcgcgagc cggctcgcga gcgccttggc gatgagcgcg gcagacagct cgtaatgagc
     1081 ggggagaagc cagttgccgg cgggcttgcc ctcggcgatc gaccactcgc cgttgtcgtc
     1141 gaacttgccg accacgcgcg gcgcgcgcat cccttcgcgc cagttgtaga gttcctcctt
     1201 gatctggtac tcgatgacct ggcgcttctc ctcgacgacg aggatctctt cgagcccttc
     1261 ggcgaagcgg cgcgccccct gcggctcgag cggccaggac atcgccacct tgtagagacg
     1321 gatgccagcg tccgcggcaa cgcgctcgtc gattccgaga tcggcgagcg cctgcagggt
     1381 atcgccgaag gacttgccgg tggtcacgat gccgaatcgg gccttcgggc tgtcccaaac
     1441 gatgcgatcc agccggttcg cgcggcagta cgcgagcgcc gcgtacacct tgtagtcgag
     1501 caggcgcgct tcctgctcga gaaaggcgtc gggccagcgg atgttgagtc ccccgggtgg
     1561 catggcgaag tccggcatca cgatgttcac gcgttgcgga tcgatgtcca ccacggcgcc
     1621 cgattcgatc acgtcggtga cgcacttgaa cgccacccag caccccgaga atcgcgacat
     1681 ggcaatgccg tgcaggccga ggtccaggta ttcctggacg ctcgaggcat tgagcaccgg
     1741 gatgcagcag gccttgaata tgtgctcgct ctggtgcgcg agggtggagg acttggccgc
     1801 gtggtcgtcg cccgccagca cgagcacgcc gccgtgcctg gacgtgcccg cggagttggc
     1861 gtgcttgaag acgtcgccgc agcggtcgac gcccggcccc ttgccgtacc acatcgcgaa
     1921 cacgccgtcg tatttcgcgt ccgggtacag gttcacctgc tgcgtgcccc agatcgaggt
     1981 ggcggcaagg tcttcgttga cgcctggctg gaatttgacg tgatgcgcgt cgaggaactt
     2041 cttcgccttc cacagcgcct ggtcgaatcc gcctaccggc gagccgcgat acccggaaat
     2101 gaatccggcg gtgctcaggc ccgcggcgag atcgcggaca cgctgcagca tcggcaggcg
     2161 caccagcgcc tgcgtaccgg tcatgaaggc gcgcccggac tgcagcgcgt atttatcgtc
     2221 gagggtgatt tcgtgatggg gggcgggggc gttcatgcgt tcctgtgtcg ggtcgctcgt
     2281 gcgtgaggcg gagcatgccg ctcattttac gcgccttgat ttgaccggac ggaggatcta
     2341 gagggttcaa gccagtgcgc cagcgccgcg cttgtttcca ccgggcgctc cagcgtggag
     2401 agatgaccgc actcctccac gatttcgagc ttggactcgg gaattcccga agccatctcc
     2461 tcgtggaggg ccagcggtat cagggcgtcc tgcctgccgc acaagacgag cgtcgggcag
     2521 gcgatgcgcg acagcagcgg caggctgtcg cttcggctga tgatcgcacg ctcctggcgg
     2581 atgaaggcat ccctccccgt attcttcgcc atcttcttga tagtagacgc taactcccgg
     2641 ttcgcgagct gcgacggatg gatcagcaag ggaagcagga catcggtgat tccgaggaag
     2701 cgcccgcgct cggcaagcgc gatgaaatcc ctgcgcttcc gggtctgctc ggccgtgtcg
     2761 gcccgggcgg tggtgtcgag cagcgcaagc t
//
LOCUS       DASPZD010000243         3474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1793211, whole genome shotgun sequence.
ACCESSION   DASPZD010000243 DASPZD010000000
VERSION     DASPZD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793211"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VEM33_04720"
     CDS             <1..589
                     /locus_tag="VEM33_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51158.1"
                     /translation="TRKVSRQVCTRIIHMDGGNLMCIELTDAQRRAELEEEERQARAR
                     REAEERAARERLGRERAEQLALQEVARKQRAAEEERLAAELRRLEAEAERQAQEERER
                     QERAAAEARARQLAEQEAREAAERECRRLAQERLDGRALLVSFVDNYGKVEENKGIAG
                     QITTFLSKARKVEPCPPANSTPQLTRTDTRLAKPR"
     gene            complement(750..825)
                     /locus_tag="VEM33_04725"
     tRNA            complement(750..825)
                     /locus_tag="VEM33_04725"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(790..792),aa:Asn,seq:gtt)
     gene            1063..1992
                     /locus_tag="VEM33_04730"
     CDS             1063..1992
                     /locus_tag="VEM33_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51159.1"
                     /translation="MLASVANAQESTWPSRTVQVVVPYTPGTGADILARILGPKLAER
                     WKIAVVTDNRAGASGNIGTDFVAKSAPDGYTLLCTATSFGTNPAINQKLPFDPIKSFA
                     PVILLATSTMGVIVTQGLPAKSMREFLELARSQPGKLYYSSPGNGGPQHLAMELLKLD
                     ARIDLVHVPYKGSGGALADLVGGHVQAMIVSLQTAAPYVQSGRLRMLAVMSAERSQAF
                     SEVPTLKELGLADLEVETWYGVFAPAGIPQAVIARVNAEMNALLEQSEIRELLAKQGM
                     APAGGTPERFGGLVKKELARWPRVVAAAGIKAD"
     gene            2032..2745
                     /locus_tag="VEM33_04735"
     CDS             2032..2745
                     /locus_tag="VEM33_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYS51160.1"
                     /translation="MSHTEDLVAAYRILAEHGVIDAYGHVSVRSDRDPKRYLLARSLA
                     PELVMPEDIMEYDLDSNPIDPRGRESVRERFIHGEIFKARPDVNAVVHNHSPSVVPFS
                     VTAVAMRPLFHMAAFVGEGVPNFEIRDAEKGTDLLVKTPYLGQALAKTLGRSPAALMR
                     GHGSVTVGESLPRAVGRSIYLEMSARMQLQAIAIAGAGGKITYLDEQEVRASVPNQDY
                     ARAWPMWRAHALARLKPQA"
     gene            2777..>3474
                     /locus_tag="VEM33_04740"
     CDS             2777..>3474
                     /locus_tag="VEM33_04740"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HYS51161.1"
                     /translation="MIFQRLAQQLAELERRGLRRSRLVRQGPQGPRIVADGQEVLAFC
                     SNDYLGLASHPRIVEAAIEAASRYGVGEGASHLLSGHSTAHERLETKLAEFMQMPRAL
                     LFSTGYQANIGAVTALAGPEDAIFSDELNHASLIDGVRLSRARVVRYPHVDIGFLSEA
                     LAESDATTKLIVTDGVFSMDGDIAPLPAVLDLCERHDAWLLVDDAHGFGVIGPGGRGA
                     LAHFGLRSPRLVYVG"
BASE COUNT          623 a         1136 c         1156 g          559 t
ORIGIN      
        1 gacgcgcaag gtgagcaggc aggtctgcac cagaatcatc catatggacg gcggcaacct
       61 gatgtgcatc gagctcactg acgcccagcg gcgcgccgag ctcgaagaag aagagcggca
      121 ggccagggcc cggagggaag ccgaggagcg cgccgcgcgc gagcgcctcg ggcgtgagcg
      181 cgccgagcag cttgccctgc aagaggtcgc ccggaagcag cgggctgccg aggaggagcg
      241 gctcgccgct gagcttcgcc ggctggaggc cgaggccgag cgccaggccc aggaagagcg
      301 cgagcgtcag gagagggcgg cggcagaagc ccgtgcccgc cagcttgccg agcaggaagc
      361 gcgtgaggca gccgagcgtg agtgtcggcg tctcgcgcag gaacggctcg acggccgcgc
      421 tctactcgtg agcttcgtcg acaactacgg caaggtcgag gaaaacaagg ggatagctgg
      481 gcagatcacc acgttcctca gcaaggctcg caaggtggag ccatgcccgc cagcaaattc
      541 gacaccgcag ctaacccgga cagatactcg gttagccaag cctcgctagc ggtagacgcg
      601 atacctacct ttactcgagc ttctttccaa cgggcggcag ctacgcgctc ttctgatgcc
      661 gacttgcgct agtgcatcct gggcgcaaca cgcgagcatc cagatgcggc tttctgcacc
      721 cgcccgtgtt ccctaactac gtgatttggt ggctccccga ccagggatcg aacctgggac
      781 ctgcggatta acagtccgtc gctctaccag ctgagctatc ggggaatcga ggctccggcg
      841 ggcgcgagac cggagattgg aaggcgcgta ttctaattcg cgcctggggc gccggtcaac
      901 gcagagaatc ggaaaaatgc cgttccggca ctcgcattct cggcttgcag acgaacctcg
      961 cgcctcttag gcataatgat cccgtgacca tcgcgagacc catcgatgat ccgagcgcgt
     1021 gaaagggcac tgtgccacgt cgcgcaaggc ctagcgctgc tcttgctcgc ctcagtcgcg
     1081 aacgcgcaag aaagtacgtg gccgagcagg acggtccagg tcgtcgttcc ctacacgccg
     1141 ggaacgggcg cggacatcct cgcgcggatt ctcgggccga aactcgccga gcgctggaag
     1201 atcgccgtcg tcaccgacaa tcgcgcgggt gcttcgggca acataggaac cgacttcgtc
     1261 gcgaaatccg cgcccgacgg ctatacgctg ctttgtaccg cgacgtcctt cgggaccaat
     1321 cccgcgatca accagaagct ccccttcgac cccataaaga gctttgcgcc ggtcatattg
     1381 cttgccacca gcacgatggg ggtgatcgtc acgcagggac tcccggcaaa gtcgatgcgc
     1441 gaattcctcg agcttgcgcg cagccagccg ggcaagctct attactcttc tccgggaaac
     1501 gggggcccgc agcatctcgc gatggagttg ctcaagctcg acgcgcgcat cgacctcgtg
     1561 cacgttccgt acaagggatc gggcggcgca ctggccgatc tggtcggcgg gcatgtgcag
     1621 gcgatgatcg tgtccttgca gacggcagcc ccctacgtcc agagcgggag gctccgcatg
     1681 ctcgccgtga tgagtgcgga gcgctcgcag gcgttttccg aggtgcctac gctcaaggag
     1741 ctgggcctcg cggacctcga ggtggagaca tggtatggcg tgttcgcgcc agccgggatc
     1801 ccgcaggcgg tcatcgccag ggtaaacgcg gaaatgaacg cgctgctcga gcaaagcgag
     1861 atccgcgagc ttctcgcaaa acagggcatg gcgccggccg gcggcacgcc cgagcgtttc
     1921 ggcggcctcg tgaagaagga attggcgcgc tggccgcgcg tggtcgcggc cgcaggcatc
     1981 aaggcggact gagctcaagc ggatttcacg gatcacccga caactgagca catgagccac
     2041 accgaagacc tggtcgccgc ataccgaatc ctggccgagc acggcgtcat cgatgcctac
     2101 ggacatgtaa gcgtgcgctc ggaccgcgat cccaagcgct atctcctggc gcgctcgctt
     2161 gctccggaat tggtgatgcc cgaggacatc atggagtacg acctcgacag caacccgatc
     2221 gatccgcggg gaagagagtc ggtgcgcgag cgcttcattc acggcgagat cttcaaggcg
     2281 cgtccggacg tgaacgcggt cgtgcacaat cattccccgt cggtcgtgcc attcagcgtc
     2341 acggcggtgg cgatgcgccc gcttttccac atggccgcgt tcgtgggaga gggggtcccg
     2401 aacttcgaga tccgcgatgc ggagaagggc accgatttgc tggtgaagac gccctatctg
     2461 gggcaggcgc tcgccaagac gctgggacga tcgccggcgg ccctgatgcg cggtcacggc
     2521 tcggtcaccg tgggcgagag cctcccccgc gcagtggggc gcagcatcta tctcgaaatg
     2581 agcgcgcgaa tgcagctcca ggcgatcgca atcgcgggtg ccggcggcaa gatcacctac
     2641 ctcgacgagc aggaggtccg cgcctccgtc cccaatcagg attacgctcg cgcgtggccc
     2701 atgtggcggg cccatgcgct tgcccgcctc aaaccgcaag cgtgaaccgc gcggcttttc
     2761 gcggcacggt cagcccttga tctttcaacg cctcgcccag cagctcgccg agctcgagcg
     2821 ccgcggcctg cgtcgcagcc gccttgtccg ccagggcccg caggggccgc gaatcgtcgc
     2881 cgacgggcag gaggtcctcg cgttctgcag caacgactac ctggggctcg ccagccaccc
     2941 gcggatcgtg gaggcggcga tcgaggccgc gtcgcgttac ggggtcgggg agggcgcctc
     3001 gcatcttttg agcggccaca gcaccgcgca cgagcgcctc gagacgaagc tcgccgagtt
     3061 catgcagatg ccgcgggctc tgcttttttc caccggatat caggcgaaca tcggtgcggt
     3121 caccgcgctc gcgggcccgg aggacgcgat tttctccgat gagcttaacc acgcctcgct
     3181 gatcgacggc gtgcgcctgt cgcgcgcccg ggtggtccgc tatccgcacg tggatatcgg
     3241 gttcctgtcg gaagcgctgg ccgagtccga cgccacgacg aaactgatcg tcaccgacgg
     3301 tgtattcagc atggacggcg acatcgcgcc cctgccggct gtgctcgatt tgtgcgaacg
     3361 tcacgatgcc tggctcctgg tcgacgacgc gcacggcttc ggcgtgatcg gccccggagg
     3421 ccgcggggcg ctggcgcact tcggcctgcg ctccccgcgc ctggtctacg tcgg
//
LOCUS       DASPZD010000244         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_846941, whole genome shotgun sequence.
ACCESSION   DASPZD010000244 DASPZD010000000
VERSION     DASPZD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846941"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..917)
                     /gene="rpoH"
                     /locus_tag="VEM33_04745"
     CDS             complement(75..917)
                     /gene="rpoH"
                     /locus_tag="VEM33_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HYS51162.1"
                     /translation="MTRTLSLPVPVGSLESYIQAVNRFPMLSFEEERDLARKFRSEND
                     VEAARQLVLSHLRLVVAIARGYMGYGLPQGDLIQEGNIGLMKAVKRFDPERGVRLVSF
                     AVHWIRAEIHEFVLRNWRMVKVATTKAQRKLFFNLRSMKPSLAPLTQTQIGSMSKQLR
                     VKPEEVAEMEMRLAGQEISLEPASDEDEPYAPIAYLADRAPEPSEIAETKQDESLRAR
                     GLETALASLDARSRRIIEARWLREKDRATLHELAAEFKVSAERIRQIEAKALAKMKDV
                     IAAA"
     gene            complement(1028..1936)
                     /gene="ftsX"
                     /locus_tag="VEM33_04750"
     CDS             complement(1028..1936)
                     /gene="ftsX"
                     /locus_tag="VEM33_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYS51163.1"
                     /translation="MRAWLQHHGLSLALTLARLARSPFATTLNVLAIGVAAALPFGVY
                     CVLGNFESLSRNASVDPQLSVFLARDAAKPEVAAVEARLKGARGVSAVRFVSREAALV
                     ELNRVAGMQEVIASLPQNPLPDAFVVTLSGSDPALADRLELEFKSLSKVAYVQADSAW
                     VRRLDALLRLGRTAVVLLSALLGLALVAVTFNTIRLQILTQRDEIELCRLIGATHAYI
                     RRPYFYLGSLLGLFGGIAALVIVLSGLTVLNRDLADLAYLYGSTVSLRFPSPNEMISA
                     LLAAVALGWLGAYLSVSRHLLETDRD"
     gene            complement(1933..2592)
                     /gene="ftsE"
                     /locus_tag="VEM33_04755"
     CDS             complement(1933..2592)
                     /gene="ftsE"
                     /locus_tag="VEM33_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HYS51164.1"
                     /translation="MAIISFRQVSKRYPGGFEALKAVSFTIEPGEMLFLTGHSGAGKT
                     TLLKLIAAIERANGGAVLVNGQNLSALRPAAIPYLRRNLGLVFQGQKLLFDRSVFDNV
                     MLPLIVTGQPYREAARRARAALDKVGLLQREKANPVALSGGEQQRLCVARAVVNRPAI
                     LLADEPTASLDAAYADEIMEMFTAFNQVGVTVLVATHDRQLIGKYRKRVLTLDHGKLL
                     Q"
     gene            complement(2595..3512)
                     /gene="ftsY"
                     /locus_tag="VEM33_04760"
     CDS             complement(2595..3512)
                     /gene="ftsY"
                     /locus_tag="VEM33_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYS51165.1"
                     /translation="MLELSSENDRRQRSWAARLKDGLAKTRSQLSGLLGGGAIDPALF
                     ESLESALLASDVGVETTRFLLTRLRERARRLQTAEQLKAELRAMLVEMLSPLEQPLDL
                     SGKKPCIIMLAGVNGAGKTTTIGKLARRFHNQGLSVLLAAGDTFRAAAREQLAAWGGD
                     AVVVAQSGGDPGAVIFDAIAAARARGIDVVIADTAGRLPTQLHLMEEAKKIKRVIAKA
                     ETGAPHEVWLVLDANTGQSSVSQIKAFDEALGLTGLILTKLDGTAKGGAVCAIARQRP
                     LALRFIGVGEGADDLRPFVAGEFVAALLD"
     gene            3575..>4476
                     /locus_tag="VEM33_04765"
     CDS             3575..>4476
                     /locus_tag="VEM33_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYS51166.1"
                     /translation="MVPARAAFLALALASGAQANPHEFTLPNGLRLIVKEDHRAPTVV
                     HQVWYRVGSMDEAPGTTGVAHVLEHMMFKGTRRFGPGEFSRLVAEAGGRENAFTSTDF
                     TVYHEQVHRDRLPLAIELEADRMANLAIRPEEFAREIKVVMEERRRSYEDRPRSLLVE
                     NLLATAFAAHPYKWPTIGWMQDLENMSWRDARDWYRHWYAPNNAVVVVVGDVAPDKVL
                     AWARRYYGALAAKAVPARKTLGEPPQRGLKRVAVKAPAELPYVRLGYKAPVLRDIAKD
                     WEPFAISLLAELLGGSDAARLHQVL"
BASE COUNT          766 a         1464 c         1491 g          755 t
ORIGIN      
        1 gcgcgcccgc gcggcttcaa aaaaaaagcc gcgggctcgc cgcggcttcg cagggactgc
       61 gcggcaaacc gtacttatgc cgcggcgatc acgtccttca ttttcgcaag cgctttggcc
      121 tcgatctggc ggatgcgctc ggcagagacc ttgaactcgg cggcgagctc gtgcagcgtt
      181 gcccggtcct tttcccgcag ccagcgcgct tcgatgatgc ggcggctgcg cgcatcgagg
      241 ctcgccaatg cggtctccag gccgcgcgcg cgcagagact catcctgctt cgtctcggcg
      301 atctcagagg gctccggcgc gcggtcggcg aggtaggcga tcggcgcgta cggctcgtcc
      361 tcgtcgctcg ccggctccag tgaaatctcc tggcccgcga gccgcatctc catttcggcg
      421 acctcctcgg gcttgacgcg aagctgcttg gacatggacc cgatctgagt ttgcgtcagc
      481 ggggcgagcg aaggcttcat gctgcgaagg ttgaagaaga gctttcgctg cgccttggtg
      541 gtggcgacct tcaccatgcg ccagttgcgc agtacgaact cgtggatctc ggcgcgtatc
      601 cagtgaaccg cgaacgatac gagccgtacc ccgcgttccg ggtcgaagcg cttgaccgct
      661 ttcatcagac cgatgttgcc ttcctggatc agatctcctt gcggcaatcc gtaacccatg
      721 tagccgcggg cgatggcgac caccaggcgg aggtgcgata gcaccagctg gcgcgctgct
      781 tcgacgtcgt tctcggacct gaatttgcgc gcgaggtcgc gctcttcctc gaacgaaagc
      841 atcggaaagc gattcaccgc ctggatgtag ctttccaggc ttcctacggg tacgggcagt
      901 gatagggtgc gagtcatgtg ttattcctcc agcgctgttg ccagaatttt agcactcagg
      961 gttttagagt gctaagggca tcacccggtt cccgaaatct gatgccttga gatgtctcta
     1021 cctaagccta atcacggtca gtttcgagta agtgtctgct cactgataga taagcaccca
     1081 gccaacccaa agcgacggcc gcaagcagcg ccgagatcat ctcgttcggg ctcgggaatc
     1141 tcaggctgac ggtggagccg tacaaatacg cgagatccgc cagatcgcga ttcaggaccg
     1201 tgaggccgga aaggacgatc accagggcgg cgatccctcc gaacaggccg agcagcgacc
     1261 cgagatagaa atagggccgg cggatgtagg cgtgcgttgc gccgatcaga cggcacagct
     1321 cgatctcgtc gcgctgagtg aggatttgca gacggatggt gttgaaggtc accgccacga
     1381 gcgcaaggcc gagcagcgcc gagagcagga ccaccgcggt ccgacccagg cgcaacaggg
     1441 catcgaggcg tctcacccac gccgaatccg cctgaacgta ggcgaccttc gacagcgact
     1501 tgaactcgag ttccagccgg tccgcgagcg caggatcgct cccagagagc gttacgacga
     1561 aggcatccgg aagcggattt tgcggcaggc tcgcgatcac ttcctgcatc cccgcgacgc
     1621 ggttgagctc cacgagcgcc gcttcccgcg acacgaaacg cacggcgctc acgcccctgg
     1681 cgcctttcag ccgcgcttcg acggccgcga cctcgggctt cgcggcgtct ctcgcgagga
     1741 acacggagag ctgcgggtcg accgaggcgt tgcgggacaa ggactcgaaa ttgcccagca
     1801 cgcaatagac cccgaacggg agcgcggccg cgaccccaat cgccagcacg ttgagcgtcg
     1861 tggcaaaggg cgaccgcgcc aggcgcgcga gcgtcagcgc gagactcagg ccgtgatgct
     1921 gcagccaggc cctcattgga ggagtttgcc gtggtccagc gtcaggacgc gtttgcggta
     1981 cttgccgatc agttgccgat cgtgcgtggc aaccagcacg gtgactccga cctggttgaa
     2041 ggcggtgaac atctccatga tctcatcggc gtaagcggcg tccaggctgg cggtgggctc
     2101 atccgcgagc aggatcgccg gccggtttac caccgcgcgc gcgacgcaca ggcgctgctg
     2161 ctcgccgccc gagagcgcca cgggattggc tttctcgcgc tgaagcaggc cgaccttgtc
     2221 gagcgcggcg cgtgcgcggc gcgccgcttc gcggtagggc tgcccggtaa cgatcagcgg
     2281 cagcatcacg ttgtcgaata cgctcctgtc gaagagcagc ttctgcccct gaaacacgag
     2341 gcccagattg cgccgcaggt aagggatcgc cgccggacgc aaggccgaga ggttctggcc
     2401 gttgacgagc accgcgccgc cgttggcgcg ctcgatcgcg gcgatcagct ttagaagcgt
     2461 cgtcttgccg gcaccggagt ggccggtgag gaacagcatc tccccgggct cgatggtgaa
     2521 gctgactgcc ttgagcgcct cgaatccccc gggatagcgc ttgctgacct ggcggaacga
     2581 gatgatggcc atggtcagtc gagaagcgcc gcaacgaact cgccggcaac gaacgggcgc
     2641 agatcgtcgg cgccctcgcc gacgcctatg aatcgcagcg ccagcggacg ctgccgggcg
     2701 atggcgcaca ccgcgccgcc cttggcggtg ccgtcgagct tggtgagaat cagcccggtg
     2761 agaccgagcg cctcgtcgaa cgccttgatc tggctgacgg agctctgccc ggtgttggcg
     2821 tccagcacga gccagacttc gtgcggggcg ccggtttccg ccttggcgat gacccgcttg
     2881 atcttcttcg cctcttccat caggtgcagc tgcgtgggaa ggcgccccgc ggtgtcggcg
     2941 atcaccacgt cgatcccgcg cgcgcgcgcc gctgcgatcg cgtcgaagat cactgctccc
     3001 ggatcgccgc ccgattgcgc gacgaccacc gcgtcgccgc cccaggcagc gagctgttcg
     3061 cgggccgcgg cgcgaaaggt gtcgccggcg gcgagcagca ccgagaggcc ctggttgtga
     3121 aaacgtcttg cgagcttccc gatcgtagtc gtctttcccg cgccgttgac gccggccagc
     3181 atgatgatgc aaggcttctt cccgctgaga tcgagcggct gctcgagcgg cgagagcatc
     3241 tcgacgagca tcgcccgcag ctcggccttg agctgctccg cggtttgcag gcgacgggcg
     3301 cgctcgcgca ggcgcgtaag caggaaccgc gttgtttcga cgccgacgtc gcttgccaga
     3361 agcgcggatt cgagcgactc gaacaacgcc ggatcgatcg ccccgccgcc gaggagcccc
     3421 gagagctgcg agcgcgtttt cgcgagcccg tccttgaggc gcgccgccca ggagcgctgc
     3481 cggcggtcgt tttcggagga gagctcgagc atgggaggaa cggatcggcg aggaaccgct
     3541 atagaatgag cgttggattt tagcatccgt cgcaatggtc cccgcacgcg ccgcctttct
     3601 cgccctcgcg ctcgcttccg gcgcgcaggc gaacccgcac gagttcaccc tgccgaacgg
     3661 cttgcgcctg atcgtgaagg aagaccatcg cgcgcccacc gtcgttcatc aggtatggta
     3721 ccgggtcggc agcatggacg aggcgcccgg cacgaccggt gtcgcgcacg tgctcgagca
     3781 catgatgttc aaggggaccc ggcgcttcgg tccgggcgag ttctcacgcc tcgtcgcgga
     3841 ggcgggcggg cgcgagaatg ctttcacgtc cacggatttc accgtctacc acgagcaggt
     3901 gcaccgcgac cgcctgcccc tggcgatcga gctcgaggcc gatcgcatgg cgaacctcgc
     3961 gatccgcccg gaggagttcg cgcgcgagat caaggtggtg atggaggagc gccgccgcag
     4021 ctacgaagac cgtccgcggt cgcttctcgt cgaaaatctt ctcgccaccg ctttcgccgc
     4081 gcacccctac aagtggccga cgatcggatg gatgcaggac ctggagaaca tgagctggcg
     4141 cgatgcacgg gactggtacc ggcattggta tgcgcccaac aacgccgtcg tcgtcgtggt
     4201 cggcgacgtc gcaccggaca aggtgctcgc ctgggcgcgg cgctattacg gcgcgctcgc
     4261 ggccaaagcg gtccccgcgc gcaagacgct gggcgaaccg ccccaacggg ggctgaagcg
     4321 cgttgcggtg aaggccccgg cggagcttcc ctacgtgcgc ctgggttaca aggcgccggt
     4381 gctgcgcgat atcgcgaagg actgggagcc gttcgcgatt tcgcttctcg cggagctgct
     4441 cggcggaagc gacgcggcgc gcctgcacca ggtcct
//
LOCUS       DASPZD010000245         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_759972, whole genome shotgun sequence.
ACCESSION   DASPZD010000245 DASPZD010000000
VERSION     DASPZD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759972"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VEM33_04770"
     CDS             <1..735
                     /locus_tag="VEM33_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYS51167.1"
                     /translation="GQPAALSVATHLAMYRRDFVFACAASALAASLPATPADASPRFY
                     ARARLIDPSGRPLEAHALPANRNFIFHYPFAGTPCFLLNLGKPTKVSARLKTAGEQTY
                     EWPGGVGADQSIVAYSAICAHRLTYPTREISFISYRNETSPGSKFAHVIHCCSEHSQY
                     DPAEGAKVLAGPAPQPLAAIVLEHDRKDDGLYAVGTLGGELFNEFFSKYEFRLALDHG
                     GHARTVVQSRCVVSELAQYCRQQVKC"
     gene            743..1453
                     /gene="aat"
                     /locus_tag="VEM33_04775"
     CDS             743..1453
                     /gene="aat"
                     /locus_tag="VEM33_04775"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HYS51168.1"
                     /translation="MIPWLERDAPFPPVQSALKDPNGLLAAGADLSSERLLEAYRHGI
                     FPWFSEGDPILWWSPDPRMILFPAELKISRSFGKVLRNRSYETRFDSAFDEVLAGCAA
                     PRKGSPGTWISGAMIEAYRSLHRLGYAHSVETWIDGELAGGLYGVAIGRVFFGESMFS
                     RSHDASKIALAALVAHLESAGFGLIDCQMHTRHLETLGAREIPRRRFSRLLEELVHYS
                     NSPGSWSGASVAQVQCRS"
     gene            1441..2181
                     /locus_tag="VEM33_04780"
     CDS             1441..2181
                     /locus_tag="VEM33_04780"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HYS51169.1"
                     /translation="MSKLNDLPHAVLQFYVTAPYPCSYLPGRMARSQVATPSHLIGTE
                     LYADLVRLGFRRSGIFTYRPYCDHCRACVPVRVPVEEFRASRSQRRALAKHAELQHAV
                     TGLRFRVEHYSLYLRYQASRHSGGGMDQDSRDQYSHFLLQSRVSTRLVEFRDKGKLMM
                     VSIVDQLGDGLSSVYTFYDPEAAGASFGTYNILWQIEQCHRARLPYLYLGYWIKDSRK
                     MSYKAHFQPIEGFIDGSWRRLEQDEVRP"
     gene            2218..>2516
                     /locus_tag="VEM33_04785"
     CDS             2218..>2516
                     /locus_tag="VEM33_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HYS51170.1"
                     /translation="MLYRAVRPLLFALDPETAHRFSLKSLDFLHHLGVASQLLRPALL
                     AGSISQPTVHVMGLDFPNPVGLAAGLDKNGEHIDGLATLGFGFLEIGAVTPRPQPG"
BASE COUNT          408 a          851 c          777 g          480 t
ORIGIN      
        1 gggcaaccgg ccgcccttag cgttgcaaca caccttgcca tgtacagacg cgatttcgtg
       61 ttcgcctgcg ccgcctcggc gctcgccgct tcgctgcccg cgacgcccgc cgacgcgagc
      121 ccgcgcttct atgcccgcgc ccggctcatc gacccgtcgg gccggccgct cgaggcgcac
      181 gcgctccccg caaaccggaa tttcatcttt cactacccgt tcgccggaac accctgcttt
      241 ctgctgaacc tcggtaagcc gaccaaggtg tcggcgcggc tgaaaaccgc aggcgagcag
      301 acctatgagt ggccgggcgg cgtgggtgcg gaccaaagca tcgtggctta ttcggcgatc
      361 tgcgcgcatc ggctgaccta ccccacccgc gagatcagct tcatcagcta tcgcaacgag
      421 acgtcgcccg gaagcaagtt cgctcatgtc atccattgct gctccgagca cagccagtac
      481 gatcccgccg agggcgcgaa ggtattggcg ggccccgcac ctcagccgct cgcggcgatc
      541 gttctcgagc acgaccggaa ggacgacggc ctgtacgccg tcggaaccct gggtggggag
      601 cttttcaacg agtttttcag caagtacgaa ttccgcctcg ctctcgatca cggcgggcat
      661 gcccgtactg tcgtccagag ccgctgcgta gtgagcgagc tcgcgcagta ttgcaggcag
      721 caggtgaagt gctgaaagcg ggatgattcc ctggctggag cgcgacgcgc ccttcccgcc
      781 ggtccagtcg gcgctcaagg atccgaacgg gttgcttgcc gcaggcgccg atctgtccag
      841 cgagcgcctg ctcgaggcct atcgccacgg catctttccc tggttctcgg aaggcgatcc
      901 gatcctgtgg tggagcccgg acccgcgcat gatcctgttt ccggcggagc tcaagatttc
      961 gcgctcgttc gggaaagtcc ttcgcaatcg ttcctatgaa acgcgcttcg attcggcgtt
     1021 cgacgaggtc cttgccggct gcgccgcgcc ccgtaaaggc tcgcccggca cctggatcag
     1081 cggcgcgatg atcgaggcct accgcagcct gcaccggctc ggatacgcgc attcggtcga
     1141 aacctggatc gacggggaac tcgccggagg actctacggc gtggcgatcg gccgggtgtt
     1201 cttcggcgag tcgatgttct cccgctcgca cgatgcctcg aagatcgcgc tcgcggctct
     1261 cgtcgcccat ctagaatcgg cggggttcgg gcttatcgac tgccagatgc acacgcggca
     1321 cctggagacg ctgggcgcgc gcgagatccc caggcgccgg ttttccaggt tgctcgaaga
     1381 gttggtacac tactcgaact cgccggggag ctggtccggc gcatctgtcg cgcaggtgca
     1441 atgtcgaagc tgaacgatct gccgcatgcc gtactgcagt tctatgtgac tgcaccttac
     1501 ccctgcagct atctgccggg ccggatggcg cgctcgcaag tggcgactcc cagccatctg
     1561 atcggcaccg aactctacgc ggacctcgtg cggctcggct ttcgccgcag cggcatcttt
     1621 acctacaggc cctactgcga tcactgccgc gcctgcgttc cggtgcgcgt ccccgtcgag
     1681 gagtttcgcg cctcgcgcag ccagcggcgg gccctggcaa agcacgcgga gctgcagcac
     1741 gcggtgacgg gactgcggtt tcgcgtcgag cactactcgc tctatcttcg ttatcaggcg
     1801 agccggcatt ccggcggcgg catggaccag gacagccgcg accagtactc gcatttcctg
     1861 ctgcagagcc gggtgagcac gcggctggtg gagttccgcg acaagggcaa gctcatgatg
     1921 gtatcgatcg tcgaccagct cggcgacggg ctttcctcgg tttacacttt ctacgatccc
     1981 gaggccgccg gcgcgagctt cggcacctac aacatcctgt ggcagatcga gcagtgccac
     2041 cgcgcgcgcc tgccctatct gtatctcggt tattggatca aggacagccg caagatgtcc
     2101 tacaaggcgc actttcagcc gatcgaaggt ttcatcgacg gcagctggcg tcgcctggag
     2161 caagacgaag tccgtccctg agcctgggtt ccgccaccgt cgcggtaaaa tgacggcatg
     2221 ctctatcgcg ccgtccggcc gcttctcttt gcgctcgacc cggaaacggc gcatcggttc
     2281 agcctcaagt cgctggactt tcttcaccat ctcggcgttg cctcgcagct tctgcggccg
     2341 gctttgcttg ccgggagcat ttcgcaaccg accgtccatg tcatgggtct ggattttccc
     2401 aaccccgtgg ggcttgcagc gggactcgac aagaacggcg agcacatcga cggactcgcc
     2461 acgctcgggt tcggcttcct cgaaatcgga gcggtgaccc cgcgtccgca gccggg
//
LOCUS       DASPZD010000246         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_633877, whole genome shotgun sequence.
ACCESSION   DASPZD010000246 DASPZD010000000
VERSION     DASPZD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633877"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VEM33_04790"
     CDS             <1..990
                     /locus_tag="VEM33_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51171.1"
                     /translation="LLILRRGGAKRRGGLALLLAALITTAAHAQPYPSRPVRLIVPFP
                     PGGSTDILSRALGQKLSEGLSQPVLIDNRPGAGGSIGSEAAAKAAPDGYTLVMGQLGP
                     LAVSPAIYKNLPYDPVKSFAPVSLMAMVPSVLVVNPQVPAASAGELIAYARANPGKLT
                     YGSAGSGSTSHLTTEYFKLATGTDILHIPYKGVGPMLTDLISGQLSMGINGAPAVMGH
                     VTSGRLRALAVTGLTRLPSLPQIPTLDESGVKGFDASGWYGILAPAGTSRAVIERLNA
                     EIRRIMKTPELRARLDNEGAIPAAGSPEEFAAFIVSEIARWGAVLKRAGIEAQ"
     gene            complement(993..1589)
                     /locus_tag="VEM33_04795"
     CDS             complement(993..1589)
                     /locus_tag="VEM33_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HYS51172.1"
                     /translation="MPGLKQVFLRTLAASSAVFFLASAAAAPEEPMLLVAKRSFEDPV
                     YGATIVLARPVQGGGHVGFIVNRPTKMSLAELFPNHEASKKVADPLFLGGTVDMNLVF
                     ALVETRGSRKDGAIRIAPDIFLAYETKAVDRIIESESDHARFFLGMVVWRPGQLDDEL
                     DRGLWFVDEPEAKLVLRRKTDGLWEELLLRLEARANTI"
     gene            1810..2079
                     /locus_tag="VEM33_04800"
     CDS             1810..2079
                     /locus_tag="VEM33_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51173.1"
                     /translation="MNLTTEHLADILIGVARAQNAVIDAMERASPGFRNTHALPLITL
                     AANMRAGDPRMIDLSARILMRLQGRVALDNAAVKADLERLTSAKP"
BASE COUNT          365 a          765 c          723 g          363 t
ORIGIN      
        1 ctcctcattt tgaggagggg tggcgcgaag cgccggggtg gtctcgctct tctcctcgcc
       61 gcgctcatca caaccgccgc gcacgcgcaa ccctatccct cgcgcccggt ccgcctgatc
      121 gttcccttcc ctcccggcgg ctcgaccgat atcctctccc gcgcgctcgg gcagaaactc
      181 tcggaaggcc tctcgcagcc ggtgctgatc gacaaccgcc ccggcgcggg cggctcgatc
      241 ggatcggagg cggcggcgaa ggccgcgccc gacggctaca cgctcgtgat gggacagctg
      301 ggcccgctcg cggtgagccc ggcgatctac aagaacctcc cgtacgaccc ggtgaagagc
      361 ttcgcgccgg tgtcgctgat ggcgatggtg ccgagcgtgc tggtggtgaa cccgcaggtg
      421 ccggcggcga gcgcgggcga gctgatcgct tatgcgcgcg cgaatccggg caagctcacc
      481 tacggctcgg cgggctcggg cagcacctcg catctcacca ccgaatactt caagctcgcg
      541 accggcaccg atatcctgca catcccctac aaaggggtcg ggcctatgct caccgatctg
      601 atctcggggc agctctcgat gggaatcaac ggcgcgccgg cggtgatggg gcacgtaacc
      661 tccgggcgcc tgcgcgcgct cgcggtgacg ggcctgacgc gcctgccgtc gctgccgcag
      721 attccgacgc tcgacgagtc gggcgtaaag gggttcgatg catcgggctg gtacggcatc
      781 cttgcgcccg cgggcacgtc tcgcgcggtg atcgagcgcc tgaacgccga gatccgccgc
      841 attatgaaga cacccgagct gcgtgcacgg ctggacaacg agggcgcgat tcccgcggcg
      901 ggctcgcccg aagagttcgc cgcctttatc gtttcggaga tcgcgcgctg gggcgcggtg
      961 ctgaagcgcg cggggatcga agcgcagtaa cgctagatgg tgttcgcccg cgcttcgagg
     1021 cgcagcagca gctcttccca gaggccgtcg gttttcctgc gcagcacgag cttcgcctcg
     1081 ggctcgtcca cgaaccaaag gccgcggtcg agctcgtcgt cgagctgccc cggccgccac
     1141 accaccatcc cgaggaagaa gcgcgcgtgg tcggactccg actcgatgat gcggtcgacc
     1201 gccttggtct cgtaggcgag gaaaatatcg ggcgcgatgc ggatcgcgcc gtccttcctg
     1261 ctccctcgcg tttctaccag ggcgaagacg aggttcatgt ccacggtgcc gccgagaaag
     1321 agcggatcgg cgactttctt cgaggcctcg tgattgggga aaagctcggc caagctcatc
     1381 ttcgtcggcc ggttgacgat gaagccgacg tggccgccgc cctgcacggg cctggcgagc
     1441 acgatggttg cgccgtagac cggatcctcg aagctgcgtt tcgccacgag gagcatcggt
     1501 tcctcgggcg cggcagccgc ggatgcgagg aaaaacaccg ccgacgaggc tgcgagggtg
     1561 cgcaagaaca cttgcttcag gccgggcatg gcgaacctcc catccggtga gcccgcgtcg
     1621 gaggacgacg ggctcctaca cgttaacgct atgcaactag cgtgccaaag gctttcgcgt
     1681 ctccttctag caggaaatca cggccgggac cttacggggc gctgaatgcg caccgcggaa
     1741 gggtgcgcgc gcccgatcgc ggggcggtgc tatcctgcct cgtccaaaca gctcatgcca
     1801 ggggaaatca tgaacctcac caccgagcac ctcgccgaca tcctgatcgg cgtcgcgcgc
     1861 gcgcagaacg ccgtcatcga tgcgatggag cgcgcgagcc ccggcttccg caacacgcac
     1921 gcgctcccgc tcatcaccct cgccgccaac atgcgcgccg gcgacccgcg catgatcgat
     1981 ctgtccgcgc gcatcctgat gcgcctgcag gggcgcgtcg cgctcgacaa cgcggcggtc
     2041 aaggcggatc tcgagcgtct gacgagcgcg aagccctgat ttccccgtac taagaaaggc
     2101 gagcacccgc tccgaaaccc ctgtatagtg cgcgccaagg ctttcgtggc gccctatccg
     2161 gttagcgctg cagcctcttc gcggttcttc tcaagttccc cgaattctca acgcag
//
LOCUS       DASPZD010000247         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_636395, whole genome shotgun sequence.
ACCESSION   DASPZD010000247 DASPZD010000000
VERSION     DASPZD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636395"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VEM33_04805"
     CDS             <1..587
                     /locus_tag="VEM33_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYS51174.1"
                     /translation="AFRRSASRMMNAIRERITRIRMTDQGCMLRAYSRDIVAAINSCR
                     EVNTFIPALAYTFAQRPTEVEVEHEERAAGDSKYSLYSLIRLNFDLVTGFSAMPLQLF
                     SLSGIAIALLSLAFVIVLAIRRAVVGPEAEGVFTLFGIAFFLIGITLFGIGLLGEYVG
                     RIYEQVRQRPRYIVQAVLERKDAVSAVPKQRQVS"
     gene            600..1520
                     /locus_tag="VEM33_04810"
     CDS             600..1520
                     /locus_tag="VEM33_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HYS51175.1"
                     /translation="MTSAVVFAYHDVGCRCLRVLLDERIEVPLVVTHGDRPDERIWFE
                     SVAELARDRAIETVAPGDPNAPEIVERIRELRPDFLFSFYYRLMLGARLLAIPGKGAF
                     NMHGSLLPKYRGRVPVNWAIIHGETETGATLHEMVEKPDAGRIVDQEAVAILPDELAA
                     DVFAKVTGAAERVLRRSLPKLVDGSAVLRAQDLSRGGYFGGRRPEDGRIDWSAPAKRV
                     HDLVRAVAPPYPGAFTEVEGAQLRLLRTRRAQKSRAGAGAFLYADGGRCYAACADGEV
                     LELVDVELDGRPLSAQEFANRIGDRRIPLH"
     gene            1529..>2393
                     /locus_tag="VEM33_04815"
     CDS             1529..>2393
                     /locus_tag="VEM33_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HYS51176.1"
                     /translation="MKRILILGVNGFIGHHLSKRILERTDWEVYGMDMQSDRVAGLLG
                     EKRFRFFEGDITINREWIEYHVRKCDTVLPLVAIATPAIYVKEPLKVFELDFEANLPI
                     VRGCVRHGKRLVFPSTSEVYGMCADREFDPEASSLVYGPIDKPRWIYACAKQLMDRVI
                     HAYGMKEGLDYTLFRPFNWIGSGLDNLNASKEGSSRVITQFFGHIVRGEPIKLVDGGK
                     QKRAFTYIDDGIDALMKIIETPKGVATGKIYNVGNPKNNYSVRQLAAMMVKLALQYPE
                     YREHVKKVKLVS"
BASE COUNT          444 a          789 c          749 g          411 t
ORIGIN      
        1 ctgcgttccg gcgcagcgca tcccggatga tgaatgccat ccgcgagcgc atcacccgca
       61 tccgcatgac cgaccagggg tgcatgctgc gcgcctacag ccgcgatatc gtcgccgcga
      121 tcaacagctg ccgcgaagtc aacaccttta tccccgcgtt ggcctatacc ttcgcgcagc
      181 gcccgaccga ggtcgaagtg gagcacgagg agcgcgccgc cggcgactcg aaatattcgc
      241 tctacagcct catccgtctc aacttcgacc tagtcaccgg gttctccgcg atgccgttgc
      301 agctcttctc gctctcggga atcgcgatag ccctgctgtc cctggcgttc gtgatcgtcc
      361 tcgccatccg ccgcgcggtg gtcgggcccg aggccgaggg cgtgttcacg ctcttcggca
      421 tcgcattctt cctcatcggc atcaccttgt tcggcatcgg cctcctgggc gaatacgtgg
      481 gccgcatcta cgagcaggtg cggcagcggc cgcgttacat cgtgcaggcc gttctggaac
      541 gcaaggacgc tgtttcggcg gttccgaaac agcgtcaagt atcgtaaccg gcacgaagcg
      601 tgacgagcgc ggttgtcttc gcctatcacg acgtcggctg ccgctgcctc agagtcctcc
      661 tcgatgagcg gatcgaagtg cctctcgtgg tgacccacgg ggacaggccc gatgagagaa
      721 tatggttcga gagcgtcgcc gagctcgctc gcgaccgggc gatcgagacc gtcgcgcccg
      781 gcgacccgaa tgcgccggag atcgtcgaaa ggatccgcga actgcgtccg gattttcttt
      841 tctcgttcta ctaccgcctg atgctcggcg cgcggctgct cgcgattccc gggaagggcg
      901 cattcaacat gcacggctcg ctccttccga agtaccgcgg gcgcgtgccg gtcaactggg
      961 cgatcatcca cggcgagacc gagaccggcg cgaccctgca cgagatggtg gagaagcccg
     1021 acgcgggacg catcgtcgat caggaagccg tcgccatcct gcccgacgag ctcgccgcgg
     1081 acgtgttcgc aaaagttacg ggagcggccg agcgcgtcct caggcgcagc ctgccgaaac
     1141 tcgtcgacgg cagcgcggtc cttcgcgcgc aggatctttc ccggggcggc tatttcggcg
     1201 gccgccgccc cgaggacgga cgcatcgact ggagcgcgcc cgcgaagcgg gtgcacgatc
     1261 tggttcgcgc cgtcgcgccg ccctatcccg gcgcattcac cgaagtggaa ggggcgcaac
     1321 tgcggctgct gcggacgcgg cgggcgcaga agagccgcgc aggggccggc gcgttcctat
     1381 acgcggacgg cgggcgctgc tacgccgcat gcgccgacgg agaggtcctc gagctcgtcg
     1441 atgtggaact ggacggccgg ccgctctccg cgcaagaatt cgccaatcgc atcggtgatc
     1501 gcaggatccc cctgcactag gagccacggt gaaaagaatc ctcatcctcg gcgtgaacgg
     1561 ctttatcggc catcatcttt cgaaacgcat cctcgagcgc acggactggg aggtctacgg
     1621 catggacatg cagtccgatc gcgttgccgg actgctcggc gagaagcgct tccgcttctt
     1681 cgaaggcgac atcaccatca accgcgaatg gatcgaatac cacgtgcgca agtgcgacac
     1741 ggtcctgccc ctggtcgcga tcgcgacgcc cgcgatctac gtcaaggaac cgctcaaggt
     1801 cttcgagctc gacttcgagg cgaacctgcc gatcgtgcgg ggatgcgtca ggcacggcaa
     1861 gcgcctggtc tttccctcga cctccgaggt ctacgggatg tgcgccgacc gcgagttcga
     1921 cccggaggcg tcctcgctcg tgtacggccc gatcgacaag ccgcgctgga tctacgcctg
     1981 cgcgaagcag ctgatggacc gcgtcattca cgcctacggc atgaaggaag ggctcgacta
     2041 cacgctgttc cggccgttca actggatcgg ctcgggactg gacaacctca acgcctccaa
     2101 ggaagggagc tcgcgggtga tcacccagtt cttcggccat atcgtccgcg gcgagcctat
     2161 aaagctcgtg gacggaggaa agcagaagcg cgcctttacc tatatcgacg acggcatcga
     2221 cgcgctgatg aagatcatag aaacccccaa gggcgtcgcg accggaaaaa tctacaacgt
     2281 cggaaatccg aaaaacaact actcggtcag gcagctggcc gcgatgatgg taaagctcgc
     2341 cctgcaatat cccgaatacc gcgagcacgt gaagaaggtg aagctcgtca gta
//
LOCUS       DASPZD010000248         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1413676, whole genome shotgun sequence.
ACCESSION   DASPZD010000248 DASPZD010000000
VERSION     DASPZD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413676"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VEM33_04820"
     CDS             <1..597
                     /locus_tag="VEM33_04820"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HYS51177.1"
                     /translation="REFEAISALDFFGSPAKERAAQALRETEGEVRKRMFPEAPNSGR
                     LTQTGRYYFKRLWASRKPLLADRLASAWLIRRFIDPEANLMWMEKTQQCPSTAVGFGF
                     DGATFSNSRDKVTFQELLAKFSLDNNETLKRIGALVHYLETGGAPVAEAAGVETLLEG
                     ARRRCNSDEELFTESEKTFDLLYEAYFDAPGKDPVHRY"
     gene            complement(620..2281)
                     /locus_tag="VEM33_04825"
     CDS             complement(620..2281)
                     /locus_tag="VEM33_04825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYS51178.1"
                     /translation="MSFKVEDAMRIVPPARATRCVSGLSPTFTIWACPDPSKWLIFLD
                     RFISPPPSDTQIRVKRRAREPNPESGIILSMRILVVCLGFALLVPRAQAQNLPELGDT
                     SGALLSPYLERRIGEQAMREIRLREPSFLDDPELTEYVNEVGGRIVAANNEARQDFEF
                     FMVRDNTINAFAMPGGFVGVHTGLLLAAQTESELAGVLAHEVAHVTQHHIARLLGKQE
                     QMSIPTLVAVVLGILAARSRPDVAQAVIAGAGASSIQSQLNYTRDFEREADRIGFQFL
                     QQAGFDVGGMGAFFERMQKATRLYENNAPAYLRTHPLTSERIADMQNRAQMAVYKQAP
                     DSIEFYLVRAKVRAEQGLPREAVAHFNELVRDHRYAEEAGARYGLASALARAREFSRA
                     SSEIETVRKLIGTHPMVELLMARVRRGAGDPAGARDVLREALARSPNYRPLHYAYVDV
                     LQGMGQHQAALAWLAELVKSYPRDARLYSMQAKSYAASGKRLLHHQALAETYVLQGTL
                     PAAIEQLQFAQKSGDGDFYQLSAVEARLRDLRSLQAEEKKTARGN"
     gene            2193..2633
                     /gene="moaC"
                     /locus_tag="VEM33_04830"
     CDS             2193..2633
                     /gene="moaC"
                     /locus_tag="VEM33_04830"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYS51179.1"
                     /translation="MVNVGDKPETHRVARAGGTIRMASSTLKLILAGSAKKGDVLGVA
                     RVAAIQAAKRTAELIPLCHSLGLTRVAAEFTAQKRQSAIRLVVTVETIGRTGVEMEAL
                     VAVSVGLLTIYDMCKAVDRAMIIEDIKLLEKSGGRSGHFRGAQP"
BASE COUNT          512 a          861 c          862 g          514 t
ORIGIN      
        1 cgtgaattcg aagcgatcag cgcgctcgat ttctttggtt cgccggcaaa ggagcgggcg
       61 gcgcaagcac tgcgcgagac cgaaggcgag gtacgaaaaa ggatgtttcc cgaagctccc
      121 aactccggcc gccttaccca gacggggcgc tattacttca agcgcctttg ggcgtcccgc
      181 aaacccctcc tggccgatcg gctcgcctca gcttggctca tccgtcgttt catcgacccg
      241 gaggcgaacc tgatgtggat ggaaaagacc caacagtgcc cttcgaccgc ggtcgggttc
      301 ggtttcgacg gcgcgacctt ttccaacagc agggacaagg taacattcca agagttgctc
      361 gcgaagttca gcctggacaa caacgagacg ctcaagagaa tcggagcgct cgtgcattat
      421 ctcgagacgg gtggtgcgcc ggtcgccgaa gcggcgggag tggaaacgct gctcgaggga
      481 gcgcgccgtc gctgcaacag cgacgaggag ctgttcaccg aaagcgagaa aacgttcgat
      541 cttctgtacg aagcctactt cgacgcgcct ggcaaagacc ccgttcaccg ctactgatcc
      601 cgaaacgcgg gcgcgttttt caatttcccc tggcagtttt cttctcctcg gcttgcagcg
      661 agcgcaggtc ccgtaagcgt gcctcgacgg cggatagttg atagaaatct ccgtccccgc
      721 tcttctgtgc gaactggagc tgctcgatag cggccggcag cgttccctgc agaacgtagg
      781 tttcggcaag agcctgatgg tgcagcaggc gcttgcccga tgcggcatag gatttggctt
      841 gcatgctgta caaccgggca tcccgtgggt agcttttcac cagctccgcg agccatgcga
      901 gcgccgcctg gtgctgtccc atgccttgca gtacatccac ataggcgtag tgaagcggcc
      961 gatagttggg ggagcgggcg agggcctcgc gcaacacgtc gcgggctccc gccggatcac
     1021 cggcgccgcg gcgcacccgt gccatcagca gctccaccat ggggtgagtc ccgatcagct
     1081 tgcgtaccgt ttcgatttcg gatgacgcgc gggaaaattc gcgcgccctt gccagagcgc
     1141 tcgccaggcc gtagcgggca ccggcctcct cggcgtaacg gtggtccctc accagctcat
     1201 tgaaatgcgc gacggcttcg cgcggcaggc cctgttcggc gcggaccttt gctcgcacaa
     1261 gataaaactc gatgctatcg ggcgcttgct tgtacactgc catctgggcg cgattctgca
     1321 tatcggcgat gcgctcgctg gtaagagggt gcgtacgcaa ataggccggc gcgttgtttt
     1381 catacagccg ggtcgctttc tgcatccgtt cgaagaacgc gcccatgccg ccgacatcga
     1441 agcccgcctg ctggagaaat tgaaagccga tcctgtcggc ctcgcgctcg aaatcacggg
     1501 tgtaattgag ctgcgattga atgctgcttg ctcctgcacc ggcgatgacc gcctgcgcca
     1561 catcgggacg ggagcgggcg gccagaatgc ccagaacgac cgccactaga gtgggaatcg
     1621 acatctgttc ctgttttccc aggagacggg cgatgtggtg ctgggtcacg tgagccacct
     1681 cgtgcgccag cacccccgcg agctcggact ccgtctgcgc tgcgagcagc aggccggtgt
     1741 gcacgcccac gaatcccccg ggcatcgcaa aagcgttgat ggtgttgtcg cgcaccatga
     1801 agaactcgaa gtcctgacgc gcctcgttgt tcgctgccac aatgcgcccg ccgacttcgt
     1861 tcacgtactc ggtaagctcc gggtcgtcga gaaagctcgg ttcgcgcaag cgaatttccc
     1921 gcatcgcctg ctcgccgatc ctgcgttcga ggtaaggcga cagcagcgca ccggacgtgt
     1981 cacccagctc cggcaggttc tgtgcctgtg ctcgcggtac caacagcgcg aagcccaggc
     2041 atacgacgag tattctcatg gataatatga taccgctctc cgggttgggt tcccgggcgc
     2101 gtcgctttac acggatctgc gtgtcgctcg gaggcgggga aatgaatcgg tcaaggaaaa
     2161 tgagccattt cgacgggtcc gggcaggccc atatggtgaa cgtcggagac aagcccgaga
     2221 cgcatcgtgt cgcgcgcgcc ggcggcacaa tccgcatggc gtcctcgacc ttgaagctca
     2281 tcctcgcggg gagcgccaag aaaggcgacg tcctcggagt ggcccgggtc gccgcgattc
     2341 aggcggccaa gcgaacggca gagctgatcc ctttgtgcca ttccctcggg cttacacgag
     2401 tcgcagcaga gttcacggcg cagaagcgcc agtccgcgat tcgcctcgtg gtgaccgtcg
     2461 agacgatcgg ccgcacggga gtcgagatgg aggccctggt agcggtcagc gttggtctgc
     2521 tcacgatcta cgatatgtgc aaagcggtgg atcgagccat gatcatcgag gacatcaagc
     2581 tgctcgaaaa atccggcggc cgctccggcc acttccgagg ggcgcagccc taggcgtcgg
     2641 agccccctgc aattcgcgcg agtcggtcac ggcttcctct cggcgcgcgg cggggccgtc
     2701 cctttgcggc ctgcaaggtc cgcaccagaa gaatccaagc gcctgatct
//
LOCUS       DASPZD010000249         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_371101, whole genome shotgun sequence.
ACCESSION   DASPZD010000249 DASPZD010000000
VERSION     DASPZD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371101"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VEM33_04835"
     CDS             complement(<1..319)
                     /locus_tag="VEM33_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS51180.1"
                     /translation="MPIKKILIVDDSATDRQFLLEVLSKQGYQCVTAQNGDEGIAKSK
                     SEMPDLILMDIIMPGTDGFKATRTITHDEATKHIPVIVCTSKKLETDRVWGMRQGARD
                     YIVK"
     gene            442..1848
                     /locus_tag="VEM33_04840"
     CDS             442..1848
                     /locus_tag="VEM33_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYS51181.1"
                     /translation="MKTLGYAIWVALGASLALRALAQPTPAQAQLREIYRELVEINTT
                     DSAGSCTAAAEAMAARLKGGGLPGQDVQVIVPPGGPKKGNLVARYRGTGAKKPVMLLG
                     HLDVVEAKREDWQRDPFKLVEEDGYFYARGTADDKAMVAVFIANLIRYRQEGYRPERD
                     LVLVATCDEEIVPSDFSGIVYLLKNHRNLVDAEFALNEGGTGLLDKNGKYLRMSIQAG
                     EKVFQTYQLEVTNPGGHSSLPVKDNAITHLAGGLARLGAHDFPFRLSATTRAYFERMS
                     RIETGQTAADMKAIMREPPDTEAIARLSATPGNNSTFRTTCVATMLDAGHATNALPQR
                     ARATVNCRILPGEPVAEVQKTLARVMANDKIKIMPTHEATLSPPPPLTPEIVGPVEAV
                     SSQMWPGVPVVPTLLAGATDGRFTINAGIPTYGVNGMFRDPDGGGVHGLNERIRVRSL
                     YEGHEFLYRLVKLYAGGS"
BASE COUNT          435 a          725 c          718 g          416 t
ORIGIN      
        1 gcttgacgat gtagtccctt gctccctgcc gcatgcccca gacccggtcg gtttccagtt
       61 tcttgctggt gcacacgatc accgggatgt gcttggtggc ttcgtcgtgc gtgatcgtgc
      121 gcgtcgcctt gaacccgtcg gtgccgggca tgatgatgtc catcaggatc aggtccggca
      181 tctcgctctt cgacttggcg atgccctcgt cgccgttctg cgcggttacg cattgataac
      241 cctgcttgct caacacttcc agcaggaact ggcggtcggt ggctgaatca tcgacgatca
      301 agatcttctt gattggcatg gcaaactctc tcctgagctg ggccggtgtc cgcggcgctg
      361 atgcaatgcc ccgcgcacac ccgttagtat atgccggtcg cacccgccac aggggcaagg
      421 tttttttcga cggagggaaa aatgaaaaca ctcggctacg cgatctgggt cgccctggga
      481 gcgtcgctcg cgcttcgcgc cctcgcgcag cccacgccgg ctcaagcgca gctgcgcgag
      541 atttatcggg agctggtcga gatcaacacc accgactcgg ccgggagctg caccgccgcg
      601 gccgaggcga tggccgcgcg cctgaaaggc ggcgggctgc cggggcagga cgttcaggtg
      661 atcgtgccgc cgggcgggcc gaagaagggc aacctcgtcg cgcgctaccg aggcaccggc
      721 gcgaagaaac ccgtcatgct gctcggacac ctcgacgtgg tggaggcgaa acgcgaggat
      781 tggcagcgcg atccgttcaa gctcgtcgag gaggacggct acttctacgc gcgcggcact
      841 gcggacgaca aggcgatggt tgcggtgttc atcgccaacc tcattcgcta ccggcaggaa
      901 ggctaccgtc cggagcgcga tctcgtgctc gttgcgacct gcgatgagga gatcgtcccg
      961 tccgatttca gcggcatcgt ctacctgctc aagaaccacc gcaatctcgt tgacgccgaa
     1021 ttcgcgctca acgaaggcgg gaccggcctg ctcgacaaga acggaaaata tctgcggatg
     1081 agcatccagg cgggcgagaa ggtgttccag acttaccagc tcgaggtgac caaccctggc
     1141 gggcacagtt cgctgccggt gaaggacaac gcgatcaccc atctcgccgg aggtctcgca
     1201 cggctcggcg cccacgactt tccgttcagg ctctccgcca ccacgcgcgc ctatttcgaa
     1261 cggatgtcgc gcatcgagac cgggcagaca gccgccgaca tgaaggcgat catgcgcgag
     1321 ccgcctgata ccgaggcgat cgcgcgcctg tcggcgacgc caggcaacaa ctcgaccttc
     1381 cgcaccacct gtgtcgccac catgctcgat gcgggacacg cgaccaacgc cctgccccag
     1441 cgcgcgcgcg ccacggtgaa ttgccgcatc ctgcctggag aaccggtcgc ggaagttcag
     1501 aagaccctgg cgcgcgtcat ggcgaacgac aagatcaaga tcatgcccac gcacgaggcg
     1561 acgctctcgc ctcccccgcc gctcacgccc gagatcgtcg ggccggtcga agccgtcagt
     1621 tcacagatgt ggcctggagt gccggtggta cccacgctgc tcgcgggagc gaccgacggc
     1681 cgcttcacca tcaacgccgg catcccgacc tacggggtga acggtatgtt ccgcgacccg
     1741 gacggcgggg gcgtacacgg tctcaacgag cgcatccgcg tgcgctcgct ctacgaaggg
     1801 cacgagttcc tttacagact ggtgaaactg tacgcgggtg gaagctagac cgtcgctcgc
     1861 aggtttcgac ctttcgattc atgacaaggc gcttgaagac tttcatccaa gcgactgtcc
     1921 cattgagatt cgaagtgaaa agacaagcgc cctgcggttt tcagcccttt gtcaggtgca
     1981 ggcttcggat tgagtgcaga cttcgcagca aggcgctacg tagggtttat cagttccaaa
     2041 cgccaatagc tgtaccggag gaaagccata agttgtaaag gtgcgcgggt atgacccagc
     2101 gcacagggtt cggaacgcgc tcgtcgtatg gagcgggttt cttttcaggc gcgcaatttt
     2161 tccgggcgcc taccgcgtag aggggcggtc gagcaatgtt ccccgcaggt aacaaccaag
     2221 cgcgaggcgc gtcaagccga tgctgggtcg gcgcgcactc gcgaaagagc gttttgaatc
     2281 cacttatagg aggc
//
LOCUS       DASPZD010000250         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_729226, whole genome shotgun sequence.
ACCESSION   DASPZD010000250 DASPZD010000000
VERSION     DASPZD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729226"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..809)
                     /locus_tag="VEM33_04845"
     CDS             complement(57..809)
                     /locus_tag="VEM33_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYS51182.1"
                     /translation="MTALRVVIIDDEEPARNRLRELLEDCREELPLSIAGEAANGMEG
                     LQIVAAAEVEVVLVDIHMPEMSGIEFARHLQVLERPPAVIFVTAHDRYAVEAFEVNAV
                     DYLTKPVRAVRLLTALRKAANGARLARGVLESIDPAPRRFLSVAERGRMTLVRVSDVI
                     YLRAELKYVTVRTRTGEHLVEESLTQLEQEFTGVFVRIHRNCLVARRWIRGVERGAES
                     EGEVGWSVVLEGLDERLPVSRRQWAQVKALVS"
     gene            complement(806..1819)
                     /locus_tag="VEM33_04850"
     CDS             complement(806..1819)
                     /locus_tag="VEM33_04850"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYS51183.1"
                     /translation="MQSIRQNDLPDALPDFRNLGVISRILLSVNALALLFALARSREI
                     GEFAAKVTEIAGVLEPILLVVVILLYAGSTLLERLAYVHGLLAVLAIVLAVTAALFPF
                     QSLLGGSGEWRELAWTLGQAALGTVAIAWYFHLRNRAFSPAISEARLQALQARIRPHF
                     LFNTLNAVLSLIRKDPKRAEGALEDLAELFRTVMVDNRQLIALGDELSLCRQYLNLEQ
                     LRLGERLRVDWRLDPAIEDALVPPMLLQPLVENAVYHGIEPGAAPGVIEVRIERRADR
                     LWVHLANPYHEDYQHRQGNHMALANIRERLQLHFDVEASLDTRIADGRYEIGIAMPYR
                     TTL"
     gene            1898..3307
                     /gene="argH"
                     /locus_tag="VEM33_04855"
     CDS             1898..3307
                     /gene="argH"
                     /locus_tag="VEM33_04855"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYS51184.1"
                     /translation="MAAKPKPGKKLWSGRFAEPVSELVERYTASVRFDRRLALHDIRA
                     SLAHARMLRAVGILTAADFKAIERGLSRISREIETGRFRWSLAAEDVHVNIERRLIAL
                     VGEAGKRLHTARSRNDQVATDLRLWLREAIDEIRGLLRAVQDGLLDQAEKHAATVMPG
                     FTHLQVAQPVTFGHHLLAYFEMLERDHERLADCRRRVNCLPLGAAALAGTSFPIDRAK
                     VARELGFEGVSGNSLDAVSDRDFAIEFCACAALAMIHLSRFSEELILWSNPRFGFVAL
                     PDRFCTGSSIMPQKKNPDVPELARGKTGRVAGDLVALLTLMKGQALAYNKDNQEDKEP
                     LFDAADTLRDTLAVFAELIPGIQVNTAAMQAAAKEGFATATDFADYLVRKGLAFRDAH
                     EAVARAVRFAEKSGLSLEQLPLEEMRRFSSRVEKDVYRVLTPEGSVASRSHIGGTAPA
                     QVRAAIKRARRLLKADPAP"
     gene            3318..>3870
                     /locus_tag="VEM33_04860"
     CDS             3318..>3870
                     /locus_tag="VEM33_04860"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HYS51185.1"
                     /translation="MGGPSGELLAALQTSVSDVAGSFRIERTTPVAGGCIHRCFVLEG
                     GGRRYFAKTNDRLALDSFAAEADGLAALSAAGARVPLPLCRGQAHEHAFLVLEHLELR
                     GNGDYAALGRSLAAVHSVHGETFGWHRDNYIGGTAQLNRRSASWSDFWREARLGPQLE
                     LAGKNRLGRDLVGKGERLVEALPR"
BASE COUNT          693 a         1260 c         1240 g          677 t
ORIGIN      
        1 cggctcctgc gcgatgcaat gacgattcgt tcggaagtcc gactcaactc tcgtccctag
       61 cttaccaggg ccttgacctg cgcccactgc ctgcggctca ccggcagtct ttcgtcgagc
      121 ccctcgagga ccacggacca tccaacttcg ccctcgctct cggcgccgcg ctcgacgccg
      181 cgtatccatc gccgcgcgac caggcaattg cgatgaatcc gcacgaacac gccggtgaat
      241 tcctgctcga gctgcgtaag cgactcctcg accaagtgct cgcccgtgcg ggtccgcacg
      301 gtgacgtatt tgagctcggc tctgaggtaa atcacgtccg atacgcgaac gagagtcatg
      361 cgtccgcgct cggcaacgct caagaagcgc cgcggggccg ggtcgatgct ctcgagcacg
      421 ccgcgcgcca accgtgcgcc gtttgccgcc ttcctcagag ccgtgagaag ccgcaccgcg
      481 cgaaccggct tggtcaggta gtccacggcg ttgacctcga acgcttcgac tgcgtaccga
      541 tcgtgcgcag tcacgaaaat cacggcgggc ggccgctcca gaacctgcag atgccttgca
      601 aattcgatgc cgctcatttc aggcatgtga atgtcgacca ggacgacctc cacttcggcc
      661 gcagcgacga tttgcaggcc ctccatgccg ttcgccgctt cgcccgcgat ggaaagcggc
      721 agttcttcgc ggcagtcctc cagaagctcg cgcaggcggt tgcgggccgg ttcttcgtcg
      781 tcgatgatga ccactcggag cgcggtcata gcgtggtgcg atagggcatc gcgatgccga
      841 tttcgtagcg gccgtcggcg atacgcgtgt ccaggctcgc ctcgacgtcg aaatggagct
      901 gcaggcgctc acggatgttg gcaagcgcca tgtgatttcc ctggcgatgc tggtagtcct
      961 catggtaggg attggccaaa tgcacccaga ggcggtcggc cctgcgctcg atgcgcacct
     1021 cgatgacgcc gggcgcggct cccggctcga ttccgtgata cacggcgttt tccaccaggg
     1081 gctgcaggag catcggcggc accagagcgt cctcgatggc aggatccagc cgccaatcga
     1141 cccggaggcg ctcacccagg cgcagctgct ccaggttgag atattggcgg cacaacgaga
     1201 gctcgtctcc gagagcgatc agctgacggt tgtcgaccat gacggtgcgg aacagttccg
     1261 cgagatcctc cagagcgccc tcggctcgct tcggatcctt gcgaatcagg gaaagcacgg
     1321 cgttcagagt gttgaaaagg aaatgcggcc ggatgcgggc ctgaagggcc tgcagccgcg
     1381 cctcggaaat cgccggcgaa aatgcgcggt tgcgaaggtg aaaataccag gcgatggcta
     1441 ctgtccccag cgcggcttgg ccgagggtcc acgcaagttc gcgccactct ccgcttccgc
     1501 cgagcaggga ttggaacggg aagagcgccg cggttaccgc aagcacgatc gcaaggactg
     1561 ccagcagccc atgaacgtac gcgagccgct cgagcagcgt ggatcccgca tagagcaaga
     1621 tcaccacgac gagcaatatg ggctcgagca cccccgcgat ctcggtgacc tttgccgcga
     1681 actcgccgat ctcccgtgag cgcgcgagag cgaaaagcag ggcgagcgcg ttgaccgaaa
     1741 gcaggatgcg cgatatcacg cccagattgc ggaagtccgg cagggcgtcg ggcaggtcgt
     1801 tttgccgtat actttgcact tttgtgcgga cgcaaaagtc gatgcttgcg gcgacgagcc
     1861 gcgcgcttca cgacttcggt gcattttaca ggcaaccatg gcagcgaagc ccaagcccgg
     1921 caagaagctc tggtccgggc ggttcgccga accggtatcc gagctcgtcg agcgctacac
     1981 cgcttcggtc cgttttgacc ggcgcctcgc gctccacgac atccgggctt cgcttgcgca
     2041 cgcgcgcatg ctccgcgccg ttgggattct gacggcggcg gatttcaagg cgatcgagcg
     2101 cggcctttca aggatctccc gcgagataga aacgggacgg tttcgctggt cgctcgccgc
     2161 cgaggacgtc cacgtcaaca tcgagcggcg gctgatcgct ctcgtcgggg aggcgggaaa
     2221 gcgcctgcac accgcccgtt cgcgcaacga tcaagtcgcg accgatctgc gtctgtggct
     2281 gcgcgaagcg atcgacgaga tcagaggcct gctgcgagct gtgcaggacg gactcctcga
     2341 tcaggccgag aagcatgccg ccaccgtgat gccgggcttc acccacctgc aggtcgctca
     2401 accggtcact ttcgggcatc acctgctcgc gtatttcgag atgctcgagc gcgaccacga
     2461 gcgccttgcc gactgccgga ggcgcgtgaa ctgcctcccg ctcggggcgg ctgcgctcgc
     2521 cgggacatcc tttccgatcg accgcgcgaa ggtcgcgcgc gagttgggtt tcgaaggggt
     2581 gagtggaaac tccctggacg cggtctccga ccgcgatttc gcaatcgaat tctgcgcttg
     2641 tgctgcgctt gcgatgatcc atctctcgcg cttttccgag gagctgattc tgtggagcaa
     2701 tccgcgcttc ggtttcgtcg cactgccgga tcgcttttgc accggctcgt ccatcatgcc
     2761 ccagaagaag aaccccgacg ttccggagct cgcgcgcggc aagaccggga gagtcgcagg
     2821 cgatctcgtc gcgctgctca ctttgatgaa gggacaagct cttgcttaca acaaggacaa
     2881 tcaggaggac aaggagccgt tgttcgacgc ggcggacacc ctgcgcgaca cactcgccgt
     2941 cttcgccgaa ttgatccccg gcatccaggt gaacacggct gcgatgcagg ctgccgccaa
     3001 ggagggcttc gccacggcga cagactttgc cgactacctg gtgaggaaag ggctcgcatt
     3061 tcgcgacgct cacgaagccg tcgcgcgcgc agtgcgtttc gccgaaaaat cggggctgag
     3121 cctggaacag ttgccgctcg aggagatgcg gcgcttttcc tcccgagtgg aaaaggacgt
     3181 ctaccgcgtg ctcacgccag aaggctcggt cgcgagccgc tcccacatcg gcggcaccgc
     3241 gcccgcgcaa gtgcgtgctg cgatcaagcg cgcgcgcaga ttgctgaagg ccgacccggc
     3301 accgtgatcc cgcagcgatg ggaggcccat cgggagagct tctcgccgca ctgcagacat
     3361 ccgtttcaga tgtcgcgggc tcgtttcgaa tcgagcgcac gacgcctgtc gcgggaggtt
     3421 gcatccaccg ttgcttcgta ctcgagggcg gcggtcgcag atacttcgcc aagacaaacg
     3481 accgcttggc actcgacagt ttcgccgcgg aggccgacgg cctcgctgcg ctttccgcgg
     3541 ccggcgcccg agttcccctc ccgctgtgcc gaggacaagc gcacgagcac gcgtttctcg
     3601 tgctcgagca cctggagctg cgcgggaacg gggattacgc tgccctgggg cgctcgctcg
     3661 ccgcggtgca ctcggttcac ggcgaaactt tcggctggca ccgggacaat tacatcggag
     3721 gaacggcgca actcaaccgg cgatcagctt cctggagcga tttctggcgc gaagcgcggc
     3781 tgggacccca gctcgagctc gcggggaaga accgcctcgg gagggatctc gtgggaaagg
     3841 gagagcggct tgtcgaagcc cttccccgac
//
LOCUS       DASPZD010000251         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1333433, whole genome shotgun sequence.
ACCESSION   DASPZD010000251 DASPZD010000000
VERSION     DASPZD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333433"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VEM33_04865"
     CDS             complement(<1..445)
                     /locus_tag="VEM33_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYS51186.1"
                     /translation="MVRPAQTANRLLLGLVLGAALGSAFAQGDAKRGEYLAKAGGCTG
                     CHTEEKKDAVPFAGGRALTSPFGAFYGPNITPHPQAGIGRWTEADFFRAMREGDRPDG
                     KNYFPAFPYPSFTKIVDRDLRDLWAFLRTLPPSARANQEHDLRFPF"
     gene            complement(518..985)
                     /locus_tag="VEM33_04870"
     CDS             complement(518..985)
                     /locus_tag="VEM33_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYS51187.1"
                     /translation="MKKRLMLAGFALALGAGYTLTAFSQVKPEIMVKQRQAAMTLQGK
                     YAGPLGAMAAGKAPYNADTVAFNAAMLDALSRMPWDGFAESTKGVTVKTAALPTIWSE
                     SAKFKAAQDNFQNAVQELVKVSRGGDEATVKAAIGTVGKACGACHQDFREKQN"
     gene            complement(1092..2024)
                     /gene="secF"
                     /locus_tag="VEM33_04875"
     CDS             complement(1092..2024)
                     /gene="secF"
                     /locus_tag="VEM33_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYS51188.1"
                     /translation="MEFFKIRNDIPFMRHALAFNVVSLLTFLVAVFFLATKGLNFSIE
                     FTGGTVMEVSYEHAAEVEKIRKALDTRGYHDYSVQNFGSSRDVLIRMPLKPGQNSADL
                     SKAVMEGLTTDDRTATLRRVEFVGPQVGAELAWNGALALLITSLGIVGYLAMRFEWKF
                     GLAAIIANLHDVVIILGFFALFQWEFSLPVLAAVLAVLGYSVNESVVIADRIRENFRK
                     MRKAGVTEIIDNAITRTISRTIITHGCTQTMVTSMLVFGGAALHYFALALTIGICFGI
                     YSSVLVMAPIVRWLGIRREDLVKPLKEKKEEAVI"
     gene            complement(2029..>3697)
                     /gene="secD"
                     /locus_tag="VEM33_04880"
     CDS             complement(2029..>3697)
                     /gene="secD"
                     /locus_tag="VEM33_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYS51189.1"
                     /translation="TGIALDFTSVKVRLADTDTQLKAKDLIERALNPDAANPPYTVAL
                     NLLPNSPRWLAFINARPMYLGLDLRGGVHFLLQVDMRAAIAKRAESLAGDMRSQLRDK
                     NVRHAGISREGESIVIRFRDAETRDKARTIIAEQIPDLKLDDASAGGELRLVATIRPE
                     AQKRTQELALKQNIQTLHNRINELGVAEPVIQQQGADRVVVQLPGIQDPTRAKELLGR
                     TATLEVRMVDEDNMNPAALAAAQAGQVPFGDEFYIERNGQPLLVRKQVVLTGDRLTDA
                     QPGFDNQTQEPAVHLTLDATGARIFKEYTRENVGKRMAILLIEKGRGEVVTAPVIRSE
                     IGGGRVQISGRMTTVEANDIALLLRAGALAAPMEIVEERTVGPSLGAENIKKGFDSTV
                     YGFAALSLFIIVYYSLFGVFSALALCANMLFLIALLSMLQATLTLPGIAAIALTLGMA
                     IDANVLINERVREELRGGATPQAAIHAGYERAFGTILDSNVTTLIAGLALLIFGSGPV
                     RGFAVVHCLGILTSIFSSVVVSRSMVNLAYGTRRKLERVAIGNTAWK"
BASE COUNT          611 a         1162 c         1248 g          676 t
ORIGIN      
        1 cgaaaggaaa acgcagatcg tgctcctggt ttgcacgggc gctcggcggc agggtacgca
       61 ggaaggccca caggtcgcgc aaatcgcggt cgacgatctt cgtaaaagag gggtagggaa
      121 aggcgggaaa atagttcttg ccgtcgggcc gatcgccttc gcgcatcgcg cggaagaaat
      181 cggcctcggt ccagcgcccg atccccgcct gcgggtgcgg cgtgatgttc gggccgtaga
      241 aggccccgaa gggcgacgtg agcgcgcggc cgccggcgaa gggaacggcg tccttcttct
      301 cttcggtgtg gcagccggtg cagccgccgg ccttggcgag atattcgccc cgcttcgcat
      361 cgccttgagc gaaggcgctg ccgagcgcgg cgccgagcac aagaccgagg agcagccgat
      421 tcgcggtttg ggcaggcctg accacgccgc caagcataac aaacgacccg gaaagagaaa
      481 gccccgccgc ggcggggctt tcggagtgcg gcgattccta gttctgcttc tcgcggaaat
      541 cctgatggca ggcgccgcag gccttgccga cggtgccgat cgccgccttg acggtcgcct
      601 cgtcgccgcc cctgctcacc ttgacgagct cctgcaccgc gttctggaag ttgtcctggg
      661 ccgccttgaa cttggccgac tcactccaga tcgtcggcag agcggcggtc ttgacggtca
      721 cgcccttggt gctttcggca aacccgtccc agggcatcct gctcagggcg tcgagcatgg
      781 ccgcgttgaa cgcgacggtg tccgcgttgt agggggcctt gcccgctgcc atggcaccga
      841 gcgggccggc gtacttgcct tgcagggtca tcgccgcctg gcgctgtttg accatgatct
      901 cgggcttgac ctgcgagaaa gcggtgagcg tgtatcccgc cccgagcgcc agggcgaatc
      961 cggccagcat aagccttttt ttcattagat ttctcctcag ttgtagggac atgcggacgt
     1021 cattgtagcg gcgttcgccg catgggtggc ataacagttt ccggcggcgg tttattccag
     1081 cgacccaatg cttagatgac cgcttcctct ttcttttcct tgagcggctt caccaggtcc
     1141 tctcgccgga tgcccagcca gcgcacgatc ggcgccatga ccaggaccga ggaatagatc
     1201 ccgaaacaga tgccgatcgt gagggcgagc gcgaagtagt gcagggccgc tcccccgaac
     1261 acgagcatcg aggtcaccat cgtctgggtg cagccgtggg tgatgatggt gcgcgagatc
     1321 gtccgggtga tggcgttgtc gatgatctct gtgacgcccg cctttctcat cttccggaag
     1381 ttttcccgga tccggtcggc gatcaccacc gactcgttca ccgaatagcc gagcacggcc
     1441 agcaccgcgg ccagcacggg gagcgagaat tcccactgga acagcgcgaa gaagcccagg
     1501 atgatcacca cgtcgtgcag gttggcgatg atcgccgcca gtccgaattt ccactcgaag
     1561 cgcatcgcga gatagccgac gatgccgagc gaggtgatga gcagggcgag cgcgccgttc
     1621 caggcgagct cggcgcccac ctgcggcccg acgaactcca cacgcctgag cgtcgcggtc
     1681 ctgtcgtccg tggtcagtcc ttccatcacg gccttggaca gatcggcgct gttctggccg
     1741 ggcttcaagg gcatgcggat cagcacgtcg cgcgaggacc cgaagttctg caccgagtaa
     1801 tcgtggtagc cgcgcgtgtc gagcgccttg cggatcttct cgacttccgc ggcgtgctcg
     1861 tagctcactt ccatcaccgt gccgccggtg aactcgatcg agaaattcag gcccttggtg
     1921 gcgaggaaga acaccgcgac aaggaaagtg aggagcgaca ccacgttgaa cgccagcgca
     1981 tggcgcatga aggggatgtc gttgcggatc ttgaagaatt ccatctgcct acttccaggc
     2041 cgtgttgccg atcgcaactc gctcgagctt cctgcgcgtc ccgtaggcga ggttgaccat
     2101 gctccggctc accacgaccg aggagaaaat cgaggtgagg atgcccaggc agtgcaccac
     2161 ggcaaagccg cgcaccggcc ccgagccgaa gatgagcagc gccagtcccg cgatcagcgt
     2221 ggtgacgttg gagtcgagga tggtcccgaa agcgcgctcg tagccggcgt ggatcgcggc
     2281 ctgcggcgtg gcgcccccgc gcagctcctc gcgcacgcgc tcgttgatga ggacgttggc
     2341 gtcgatcgcc atgccgaggg tgagcgcgat cgcggcgatg cccgggaggg tgagcgtcgc
     2401 ctggagcatc gaaagcagcg cgatcaggaa cagcatgttg gcgcacagcg cgagcgcgga
     2461 gaacacgccg aacagcgagt agtagacgat gatgaaaaga gacagcgcgg cgaagccgta
     2521 cacggtcgag tcgaacccct tcttgatgtt ttcggccccg agggagggac ccaccgtacg
     2581 ctcctcgacg atctccatgg gtgcggcgag cgcccctgcc cgcaggagca gcgcgatgtc
     2641 gttcgcctcg acggttgtca tcctgccgct gatctggacg cgcccgccgc cgatctcgct
     2701 gcggatcacg ggcgcggtga cgacttcgcc cctgcccttt tcgatgagga ggatcgccat
     2761 ccgcttgccg acgttctcgc gcgtgtattc cttgaagatt cgcgcgccgg tggcgtcgag
     2821 cgtgaggtga acggccggtt cctgggtctg gttgtcgaag cccggttgtg cgtcggtgag
     2881 ccggtcgccg gtcagcacga cctgcttgcg cacgaggagc ggctggccgt tgcgctcgat
     2941 gtagaactcg tcgccgaaag ggacttgccc ggcctgcgct gcagcgaggg ctgcgggatt
     3001 catgttgtcc tcgtccacca tgcgcacttc gagggtcgcg gtgcgcccga ggagttcttt
     3061 ggccctagtc gggtcttgga tacccggtaa ttgcacgacc acgcggtcgg cgccttgctg
     3121 ctggatcacg ggctcggcga ctcccagctc gttgatgcgg ttgtgcagcg tctggatgtt
     3181 ctgcttgagc gcgagctcct gcgtgcgctt ctgcgcctcg ggccggatcg tggcgacgag
     3241 gcgcagctcg ccgcccgcgc tggcgtcgtc gagcttcagg tccggaatct gctcggcaat
     3301 gatcgtgcgc gccttgtcgc gggtctctgc gtcgcggaag cggatcacga tgctctcgcc
     3361 ttcgcgcgag attccggcgt gacgcacgtt cttgtcgcgc agttgcgagc gcatgtcgcc
     3421 ggcgagactt tccgcccgct tggcgatcgc cgcgcgcatg tccacctgca gcaggaaatg
     3481 cacgcccccc cgcagatcca ggccgaggta catcggccgc gcgttgatga aggcgagcca
     3541 gcggggagaa ttcggcagga gattgagcgc gaccgtatac ggggggttcg cggcgtcggg
     3601 attgagcgcg cgctcgatga ggtccttcgc cttgagctgg gtgtccgtgt cggcgagccg
     3661 caccttgacg ctggtgaagt cgagcgcgat cccggtc
//
LOCUS       DASPZD010000252         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_677454, whole genome shotgun sequence.
ACCESSION   DASPZD010000252 DASPZD010000000
VERSION     DASPZD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677454"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VEM33_04885"
     CDS             <1..454
                     /locus_tag="VEM33_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51190.1"
                     /translation="LTGTKLLHVPYKGSAGALTDLLSGEVNVGFVPIHVAMAHVKAGR
                     LKALAVGSAKRHPNAPDVPSLQELGVKGANVDMWYAFMAPKGTPAPVVSKLDSELRAI
                     LSLPEIKSNFEKQGMDAASSSPDELNALMRRDHARWAAVIKKNNITAE"
     gene            487..1710
                     /locus_tag="VEM33_04890"
     CDS             487..1710
                     /locus_tag="VEM33_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYS51191.1"
                     /translation="MIRSTDRILITHAGALPRSDELRRMVLARAEGQPHDAAALAAHL
                     KSEVAEVVRKQIACGIDSVNDGELGKSNFTNYVRERIAGIEIRDYRPGVDSAPLDITA
                     RDLRQFPRYFGAGKGTLTGAAMRKRLSCCVSPLNYVGHEALKEELEGFRAALQGVKVA
                     EAFLPANTPGTVEHWLRNEHYRNGEEFVYAIAEALRDEYQAIVDAGFLLQIDDPDLPD
                     GWQMYPDMSVREYRKYATLRVDAINHALRGIPREKVRLHVCWGSFHGPHRNDIPLKDI
                     IDIIFRVRASSYSIEASNPVHEHEWRVFEEVKPPEGTVLIPGVVGHCCDFIEHPELVA
                     QRLLRYAKLLGRENVMGGTDCGLGPRVGSPEIAWAKLEALAQGARLASRRLWMRPNRP
                     SKKKRAPARKKKAKR"
     gene            complement(1707..2678)
                     /locus_tag="VEM33_04895"
     CDS             complement(1707..2678)
                     /locus_tag="VEM33_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51192.1"
                     /translation="MRLKQIGRVACLVLPALVAATAQAQGSFPSKPIRIIVGFAAGGG
                     NDIIVRVLAPKMSEGLGQPIIVENKPGAQSIIAAEYVAKSAPDGYTLFMGPSGAMTMN
                     PAIYSKLPYSPLKDFAQISMIGDFPLILVVNSSLPVNSVKDLIDYAKSRPSDVNYSAS
                     AAPFQLAAELFNQKTGTKFAHIPYKSSGDSVGAVMAGQVTMTIMDPPPAIGPLKGGKV
                     KGLAVTSAKRDPAWPELPTLAEAGGPDIEIRLFTGFHAPAGTPQAIVNRIQQEVARTV
                     RLPEIKERLDQMSIVPSGNTPEEFRQIIARDIAKWTAVAKAANIKAD"
     gene            2796..>3979
                     /locus_tag="VEM33_04900"
     CDS             2796..>3979
                     /locus_tag="VEM33_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HYS51193.1"
                     /translation="MGRLISVLAAVLTCAAIALSADLFRVAGLSLYTEQVLAGLLAIA
                     TPLVFLHVSAGGERGRAAAVPWYDAAAAIAAFAASAYLAIRYASLSELTSKQPWDGLL
                     AAGVLLVLFLEGLRRTSGSALFYTTLAFIALAMFGGTLPGEFVARSIPLPRLAYYLVW
                     DSSAILGIAIKIIATVVVVYVFFGQVLLKAGGSAFFTDIAMALMGKYRGGPAKIAIVG
                     SSLFGSISGNIVSNVMTVGTVTIPLMKRAGFRPHLAAAIEACASTGGQITPPVMGIAA
                     FIMAEFLQVPFYEVALAAVIPALLFYVALFIQVDLEAAKSKILPMPKEDIPRLGRVLA
                     EGWYFLLPLVALVYALFGLNYEPEMAGLVATAIVLALGVAFPFRGKRIGLRDLYVMLR
                     DAG"
BASE COUNT          647 a         1308 c         1321 g          703 t
ORIGIN      
        1 cctcaccggc acgaagctgc tgcacgtgcc ttacaagggc agcgccggcg cgctcaccga
       61 cctcctctcc ggggaggtca acgtcggatt cgtgccgatt cacgtcgcca tggctcacgt
      121 caaggccggc aggctgaagg cgctcgcagt gggcagcgcg aagcgccacc cgaacgcgcc
      181 ggacgtaccg agcctgcagg agttgggggt aaaaggcgca aacgtggaca tgtggtacgc
      241 gttcatggcg cccaaaggca cgcccgcgcc ggtcgtctcc aagctcgact ccgagctgcg
      301 cgccatcctt tctcttccgg aaatcaagtc caacttcgag aagcagggca tggacgcggc
      361 gtcttcctcc cccgacgagc tgaacgccct gatgcggcgc gatcacgcgc gctgggcagc
      421 ggtcatcaag aagaacaaca tcaccgccga atagcgcggt tccgggatcg aacccgaaga
      481 acggcgatga tacgcagcac cgacaggatc ctcatcacgc acgcgggagc gctgccgcgc
      541 tccgacgagt tgcgccggat ggtcctcgcc cgcgccgagg gacagccgca cgacgcggcg
      601 gcgctggccg cgcacctgaa gagcgaagtg gcggaggtcg tgcgcaagca gatcgcctgc
      661 ggcatagaca gcgtcaacga cggcgagctc ggaaagagca atttcaccaa ctacgtgcgc
      721 gagcgcatcg ccgggatcga gatccgcgat taccggcctg gggtggactc tgctccgctc
      781 gacatcaccg ctcgcgacct gcggcagttt cctcggtact tcggtgcggg caaaggcact
      841 ctcaccggcg ctgcgatgcg aaagcggctg agctgctgtg tctcaccgct gaactacgtc
      901 ggccacgagg cgctcaagga agagctcgag ggtttccgcg cggcgctgca gggcgtgaag
      961 gtcgccgagg ccttcctgcc cgcgaacacg ccggggacgg tcgagcattg gctccgaaac
     1021 gagcactaca ggaacggcga ggaattcgtc tacgcgatcg ccgaagcgct gcgggacgag
     1081 taccaggcga tcgtcgatgc ggggttcctg ctgcagatcg acgacccgga tcttccggac
     1141 ggctggcaga tgtatcccga catgagcgtg cgcgagtacc gcaagtacgc gacgctgcgc
     1201 gtggacgcga tcaaccacgc gctgcgcggc attcctcgag agaaagtgcg gctgcacgtc
     1261 tgctggggga gcttccacgg cccgcaccgc aacgacattc cgctgaaaga catcatcgac
     1321 atcatcttcc gcgtgcgcgc ctcgagctac tcgatcgagg cctcgaaccc ggtgcacgag
     1381 cacgaatggc gggtgttcga agaggtgaag ccgcccgagg gcacggtgct cattcccggc
     1441 gtcgtcggcc actgctgcga tttcatcgag caccccgagc tcgtcgccca gcggctgctc
     1501 cgctacgcga agctcctcgg ccgcgagaac gtcatgggcg gaaccgactg cggcctcggg
     1561 ccgcgcgtcg gcagccccga gatcgcgtgg gcgaagctcg aagcgctcgc gcagggcgcg
     1621 cgcctggcga gcaggcgatt gtggatgcgg ccgaaccgac cgtcgaagaa aaagcgcgcg
     1681 cccgcgcgaa agaagaaggc gaaacgctag tcggccttga tgttcgcggc tttcgccacc
     1741 gccgtccact tggcgatgtc cctcgcgatg atctggcgga attcttccgg cgtattgccc
     1801 gaagggacga tcgacatctg gtccaggcgc tccttgatct ccggcagcct gaccgtccgc
     1861 gccacttcct gctgaatgcg attcacgatc gcctgcggtg ttcccgccgg cgcgtggaac
     1921 ccggtaaaga ggcggatctc gatgtccggg ccgcccgcct cggcgagcgt cggcagctcg
     1981 ggccaggccg ggtcgcgctt cgcggaggtc accgcgaggc ccttgacctt gccgcccttc
     2041 agcggaccga tcgccggcgg cggatccatg atggtcatgg tcacctgtcc cgccatgacc
     2101 gcgcccaccg agtcgccgct gctcttgtag gggatatgcg cgaatttcgt ccccgttttc
     2161 tggttgaaga gctcggcggc gagctgaaac ggtgccgcgc tcgcggaata gttgacgtcg
     2221 ctcggccttg actttgcgta gtcgatcaga tccttcaccg agttcaccgg cagcgaggag
     2281 ttgacgacga ggatcagagg gaagtctccg atcatcgaga tctgcgcgaa atccttgagc
     2341 ggcgagtagg gcagtttcga gtagatcgcc ggattcatgg tcatcgcccc gctcgggccc
     2401 atgaacagcg tgtagccgtc gggggcggac ttggcgacgt attcggcggc gatgatgctc
     2461 tgcgcgccgg gcttgttctc gacgatgatc ggctggccga ggccttccga catcttgggc
     2521 gcgaggacgc gcacgatgat gtcgttgccg ccgccggccg cgaagccgac gatgatgcga
     2581 atcggcttgc tggggaagct gccctgtgcc tgcgccgtgg ccgcgaccaa tgccggcagc
     2641 acgaggcaag cgacacgtcc gatctgtttg agtctcatga gccctcctgt gaacgcgtag
     2701 agtgccacgc cgcagcagcc gcgatcaact ccattcgcga cggcgaggaa gcacagggat
     2761 ttgcacaggc cggccgctgc ggctagagtt gtccgatggg gaggctcatc agcgtcctgg
     2821 cggccgtgct cacctgcgcc gcgatcgcgc tgtccgcgga tctcttccgc gtggcgggac
     2881 tctccctcta caccgagcag gttctcgccg gcctgctcgc gatcgccacg cctctggtgt
     2941 tcctgcacgt gtcggcgggc ggcgaacgcg gccgcgcggc cgcggtgcct tggtacgatg
     3001 ctgcggcggc gatcgcggct ttcgccgcgt cggcttatct cgcgattcgc tatgcgagcc
     3061 tatccgagct cacctcgaag cagccctggg acggactcct tgccgcgggc gtgctgctcg
     3121 tgctcttcct cgaggggctg cgccgcacgt ccggttcggc gctcttctat accacgcttg
     3181 cgttcatcgc gctcgcgatg ttcggtggaa cgctgcccgg ggaattcgtc gcgcgctcga
     3241 taccgctgcc gcgcctggct tattacctgg tgtgggattc gagcgcgatt ctcggcatcg
     3301 caatcaagat catcgcgacc gtcgtcgtgg tatacgtttt tttcggccag gtgctgctca
     3361 aggcgggcgg ctccgccttc ttcaccgaca tcgccatggc gctgatgggg aagtaccgcg
     3421 gcggtccggc caagatcgcg atcgtgggat cgtcgctctt cgggtcgatc tcgggcaaca
     3481 tcgtctcgaa cgtcatgacg gtgggcactg tgacgatccc gctgatgaag cgcgcggggt
     3541 tccggcccca tctcgcggcg gcgatcgagg cctgcgcctc gacggggggc cagatcacgc
     3601 cgcccgtcat ggggatcgcg gccttcatca tggcggagtt cctccaggtg cctttttacg
     3661 aggtcgccct ggccgcggtc atacccgcgc tcctcttcta cgtcgcgctc ttcatccagg
     3721 tggacctcga ggcagcgaag agcaagatcc tgccgatgcc caaagaggac atcccgcggc
     3781 tcggccgggt tctcgcggag ggctggtatt tcctgctgcc gctcgtggcg ctggtctacg
     3841 ccctgttcgg actgaactac gaaccggaga tggcggggct ggtggcgacc gccatcgtgc
     3901 tcgctctcgg agttgccttt cccttccgcg gcaagcgcat cggcctccgc gacctctatg
     3961 tgatgctgcg cgacgcggg
//
LOCUS       DASPZD010000253         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1142309, whole genome shotgun sequence.
ACCESSION   DASPZD010000253 DASPZD010000000
VERSION     DASPZD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142309"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VEM33_04905"
     CDS             complement(<1..406)
                     /locus_tag="VEM33_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYS51194.1"
                     /translation="MAALAPDPVAFRALEHAGWQGAARHYDDAFGSLTRQAVDPLLDA
                     AEVRSGARTLDVASGPGYAAAAAAARGARVAALDFSPAMVELARSQSPEIEFREGDAE
                     ALPFPEASFDAVVMNFGMLHLAQPERAVAEAFR"
     gene            complement(497..895)
                     /locus_tag="VEM33_04910"
     CDS             complement(497..895)
                     /locus_tag="VEM33_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS51195.1"
                     /translation="MKKLTIAAALASAALGTGIGLLNAQQPGFTRTVLQDQDLSAQGR
                     HAVVARAEFIPGGQAGRHTHPGEELGYVVEGTLQLLVDGQPPKTLKAGEVFFVPAGAV
                     HDGKNIGKGKAVVLATYVVEKGKPVATAIK"
     gene            complement(932..1957)
                     /locus_tag="VEM33_04915"
     CDS             complement(932..1957)
                     /locus_tag="VEM33_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYS51196.1"
                     /translation="MDEQRRSFLKKTGAAGVVALAAGASAPEPSLAQGAGPGASPGSP
                     AAPKRMTFVTLRSGDAYALGVKTDLGILDVLKTAKFIRSPAPITIDDLIRRGDRGLTE
                     LVKVALARRTPGFFVAEDRAEFGPCVTNPEKILCVGLNYARHARETNNPIPKLPILFN
                     KFNNALNAHQGTVRVSAVPAERFDYESELVIVMGRKARNVSEAEALSYVFGYCIGHDF
                     TARDLQSRSSQWMIGKTCDGFGLLGPYLVTADQIPDPNNLKIEGTVNGEVRQSSNTSD
                     MVHNCAVIISYASTLMTLLPGDVIYTGTPEGVIAGYPKEKQVWLKPGDRLTTTIEKLG
                     TLRFALA"
     gene            complement(1985..2371)
                     /locus_tag="VEM33_04920"
     CDS             complement(1985..2371)
                     /locus_tag="VEM33_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51197.1"
                     /translation="MAIQGMNHFNVLTDKLAATRAFYVGVLGFTEGPRPPLDFGGAWL
                     YAGGQPILHVSEGKLPADPAGVLDHLAFSATDLKGTVAKLKANAIKFTLRRQVGVGTW
                     QIFCHDPCGAKVELDFSPQESAPADT"
     gene            complement(2380..3024)
                     /gene="pncA"
                     /locus_tag="VEM33_04925"
     CDS             complement(2380..3024)
                     /gene="pncA"
                     /locus_tag="VEM33_04925"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HYS51198.1"
                     /translation="MASAPQIKPGIKDALVVVDVQNDFCPGGRLAVQKGDEVVPLVNA
                     FAGRFENVVLTQDWHPPGHRSFATSHPGSKPFDSVRLVYGEQVLWPDHCVQGSDGAAL
                     HKDLCVPHAQLVLRKGHHRDVDSYSAFLEADRKTRTGLEGYLEERGIKRVFVCGLATD
                     FCVAWTALDARKLGFAALVVEDACRAIDMQGSLAAAWEKMRKAGVKRIRSEDIV"
BASE COUNT          486 a         1086 c         1068 g          557 t
ORIGIN      
        1 cgcggaaggc ctcggccacg gcgcgttccg gctgggccag atgcagcatg ccgaagttca
       61 tcaccaccgc gtcgaagctg gcttccggaa acggcagcgc ctcggcgtcg ccctcgcgga
      121 actctatttc cggactctgc gagcgcgcga gctccaccat cgcgggggaa aaatcgagcg
      181 ccgcgacacg cgcgccgcgg gccgcggcgg ccgccgccgc gtagcccggg ccgctcgcca
      241 cgtcgagcgt gcgcgcgccc gagcgcactt ccgccgcatc gagcagtgga tcgaccgcct
      301 gccgcgtgag gcttccgaac gcgtcgtcgt agtgacgggc ggcgccctgc cagcccgcgt
      361 gctcgagcgc gcggaacgcg acggggtcgg gcgcaagcgc cgccatcggg tcagccgcgg
      421 cgcgcgccgc gcgcgcgcgc cggggcgaac gaacccggcg tgcgcacggc gcacgctccc
      481 ctttcgcgcc tgggggtcac ttgatcgctg tcgcgaccgg cttgcctttc tcgacgacgt
      541 aagtcgcgag cacgaccgcc ttgcccttgc cgatgttctt gccgtcgtgg accgcgccgg
      601 cagggacaaa gaacacctcg cccgccttga gcgtcttcgg cggctggccg tccacgagga
      661 gctgcagcgt gccttccacg acgtagccga gctcctctcc cggatgcgtg tgacgtccgg
      721 cctgcccgcc gggaatgaat tccgcgcggg ccacgaccgc gtgccggccc tgggcgctga
      781 gatcctggtc ctgcaggacc gtgcgcgtga agccgggctg ctgcgcattg agcaagccga
      841 ttccggtccc tagcgctgcg gaggcgaggg ccgctgctat cgtcagtttc ttcatgcttg
      901 gttcctcctt ttgtaggtcg tcgaaagaaa atcaggcgag ggcgaagcgc agcgtcccca
      961 gcttctcgat ggtcgtggtc agccggtcgc cgggcttgag ccacacctgc ttctccttgg
     1021 gatagcccgc gatcacgccc tccggagtgc cggtgtagat gacatcgccg ggcaggagcg
     1081 tcatcagggt cgaggcgtag ctgatgatca ccgcgcagtt gtgcaccatg tcgctcgtgt
     1141 tcgaggactg gcgcacttct ccgttcaccg tgccttcgat cttgaggttg ttcggatcgg
     1201 gaatctggtc cgcggtcacg aggtagggtc cgagcagccc gaagccgtcg caggtcttgc
     1261 cgatcatcca ctggctgctg cgcgactgca gatcgcgcgc ggtgaagtcg tgaccgatgc
     1321 agtagccgaa cacgtacgag agcgcctcgg cttcgctgac gttccgggcc ttccttccca
     1381 tcacgatcac cagctcggat tcgtagtcga agcgctcggc gggcaccgcc gacacgcgca
     1441 ccgtgccctg atgggcgttg agcgcattgt tgaacttgtt gaaaagaatg gggagctttg
     1501 gaatcggatt gttggtttcg cgcgcgtgcc tggcgtagtt gaggccgaca cagaggattt
     1561 tttccgggtt ggtcacgcac ggtccgaact cggcgcggtc ctcggcgacg aaaaaccccg
     1621 gagtcctgcg cgcgagcgcc accttgacga gctcggtgag cccgcggtcg ccgcggcgga
     1681 tgagatcgtc gatcgtgatc ggcgcggggc tgcggatgaa tttcgccgtc ttgaggacgt
     1741 cgagaatccc gaggtcggtc ttcacgccca aagcgtaggc gtcgccgctg cgcagggtga
     1801 cgaaggtcat cctcttcggc gccgcgggcg agccggggct cgcgcccggc ccggcgccct
     1861 gcgcgaggga gggctccggc gccgacgcgc ctgcggcgag cgcgacgacg cccgccgcac
     1921 ccgttttttt caggaagctt ctgcgctgct catccatcgc tctttatcct ccatcgggtc
     1981 ctgatcacgt gtccgcgggt gcggactcct ggggcgagaa atcgagctcg accttggctc
     2041 cgcagggatc gtgacaaaaa atctgccacg ttccgacgcc gacctgccgg cgcagcgtga
     2101 actttatcgc gttcgccttg agcttcgcca ccgtgccctt caagtcggtg gcgctgaacg
     2161 cgagatggtc gagcaccccg gccgggtccg cgggcagctt tccctcggag acatgcagga
     2221 tgggctggcc acctgcgtac agccaggcgc cgccgaaatc gagcgggggc cgcggccctt
     2281 cggtgaaacc gagaaccccg acgtagaacg cccgcgtcgc ggcgagcttg tcggtgagca
     2341 cgttgaagtg attcattccc tggatcgcca tgtggccccc taaacgatgt cttcggaccg
     2401 gatgcgcttt acgccggctt tcctcatctt ttcccaggcc gcagcaagcg agccctgcat
     2461 gtcgatcgcg cggcacgcgt cctcgactac gagcgcggca aaaccgagct tgcgcgcgtc
     2521 gagcgccgtc cacgccacgc agaaatcggt cgcgagcccg cagacgaaca cgcgcttgat
     2581 gccgcgctcc tcgaggtagc cctcgaggcc ggtgcgggtc ttgcggtcgg cctcgagaaa
     2641 agccgagtag ctgtcgacgt ccctgtgatg gcctttgcgc aggacgagct gcgcgtgggg
     2701 aacgcagagg tccttgtgca gggcggcgcc gtcgctcccc tgcacgcagt gatcgggcca
     2761 gagcacctgc tcgccgtaaa ccagcctcac cgaatcgaag ggcttgctgc ccgggtgcga
     2821 ggttgcgaac gatcgatggc ccggcggatg ccagtcctgc gtcaggacga cgttctcgaa
     2881 gcgcccggcg aacgcattca cgagcggcac gacttcgtcg cccttctgca cggcgagccg
     2941 gccgccgggg caaaagtcgt tctgcacgtc gacgacgacc aaggcatcct tgatcccggg
     3001 cttgatctgc ggcgccgacg ccatctccgg actttccgct accggattcg cgcgacatcg
     3061 accaggcagt cgtagaacgt cgcgcccctg ccgatgtcgg tgagcgcctg cgaggtcagc
     3121 tcgttggcgt tgcggcggtc ggcggtgagc tttctccacc acaccgaaag cgccaccacg
     3181 actccctccc gcgcctt
//
LOCUS       DASPZD010000254         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1786133, whole genome shotgun sequence.
ACCESSION   DASPZD010000254 DASPZD010000000
VERSION     DASPZD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786133"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VEM33_04930"
     CDS             complement(<1..605)
                     /locus_tag="VEM33_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51199.1"
                     /translation="MEAARASALRRGVQLEILTIAWMLIEACVAVGAGIVARSVLLTA
                     FGIDSVVELLSGIVLFRLLTGRSGETAATRISAVLLILLCAYVLLFSIAGLIFRIEPE
                     SSLAGIAVSAIAIVAMPVLAHRKRRVNAVLQSPALRADIAESISCAYLAGMTLVGLVV
                     SMLTGLWWIQYLAALGLLAWLVPEAREALLESSPSGGSAADL"
     gene            636..1376
                     /locus_tag="VEM33_04935"
     CDS             636..1376
                     /locus_tag="VEM33_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYS51200.1"
                     /translation="MGPRADGYRNELRGLKNWEPYLRKHSGLPGPRANLELVAAVAEE
                     ADADRLWRLSASHDEFLALCGTAGLGRIALIEPDAVIKWLHELASDPRWRVREGVAMA
                     LQRLGSEDMPGLLSLMRLWAREGPYLQRAAVAGLCEPAILKRNEDAVAVLVILDGITK
                     SVALASAADRRDEGFEGLRKALGYGWSIAAAAAPRNAKPYLEKWLRSTDRDVAWIMRS
                     NMRKARMDGLREQLVSSPPVRTRGKHLA"
     gene            1357..1776
                     /locus_tag="VEM33_04940"
     CDS             1357..1776
                     /locus_tag="VEM33_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HYS51201.1"
                     /translation="MANISPEAMAALESGRLAHIVTLNPDNSPQMSCVWIGVEDDEIV
                     IGSLPRNRKVQNIERDARVVLSIETGRTGNHGLAEYLVVHGRARVTRGGAPELLQRLA
                     HVYMGPDVKFPPMPNPPPGYVIRIEPRRLSGNGPWSD"
     gene            complement(1778..2209)
                     /locus_tag="VEM33_04945"
     CDS             complement(1778..2209)
                     /locus_tag="VEM33_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HYS51202.1"
                     /translation="MSRNQQRPQIPNDMRAFNAKLIEEFRANQGQMSGQMAGRQLLLL
                     TTTGAKSGRQRTAVVGYRPYGDDLAIIASDNGAPNAPQWFANLEKDPVATVEVGSEKF
                     KVRTRVASPEERPEIAKLIEYLGPQQKLTEREIPIVILERV"
     gene            2322..>2687
                     /locus_tag="VEM33_04950"
     CDS             2322..>2687
                     /locus_tag="VEM33_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51203.1"
                     /translation="MVILAIALIVALTRRSSRLRPLPAESRDRFAHTWSNLESRFIED
                     PRAAVQEADRVAVLILSERGATMPDGRDVPKGLEHARAAASSDEGRQGTEGMRKAMVH
                     YKQIVDDAIGTAKETRDASR"
BASE COUNT          488 a          838 c          904 g          457 t
ORIGIN      
        1 aggtcggcgg cggagccgcc ggatggggat gattcgagca acgcttcgcg tgcttctggt
       61 acaagccacg caagcaaccc cagcgcagcg aggtactgga tccaccacaa gccggtgagc
      121 atgctcacga caaggccgac cagggtcatc ccggcgagat acgcgcagct gatcgactca
      181 gcgatgtcgg cccgcagcgc gggactttgc aacacggcgt tgacgcggcg tttgcggtgc
      241 gccagcaccg gcatggcaac gatcgcgatg gcgctgacag cgatgccggc gagcgaggat
      301 tccggctcga tccgaaaaat caacccagcg atcgaaaaca gaaggacgta agcgcacagc
      361 aggatcaaca aaacggcaga gatacgggtc gcggcggtct ctccggatcg gccagtcaaa
      421 aggcgaaaca ggacaatccc gctcaggagc tcgactaccg agtcgatgcc gaaagcggtc
      481 agcaggacgc tgcgcgcgac gatccccgcg ccgacggcga cgcatgcctc gatcagcatc
      541 caggcgatgg tcaggatctc cagctggacg ccgcgacgga gtgcgctcgc ccgtgccgct
      601 tccatgctcg aattgtcggt taggatcgcg ggcacatggg tccgcgcgcc gacggatatc
      661 gcaacgagct tcgcgggctc aagaactggg agccctacct gcggaagcat tcggggctac
      721 cggggccacg agccaacctc gagttggtcg cggcggtcgc cgaggaggcg gacgcggacc
      781 gcctatggcg gttgtcggcc tcccacgacg agtttctggc cctctgcggc acggccggct
      841 tgggccgcat cgccctgatc gagcccgacg cggtgatcaa gtggctgcac gagctcgctt
      901 cagacccgcg ttggcgggtg cgcgagggcg tggcgatggc gctccagcgt ctgggctcgg
      961 aggacatgcc cgggctgctt tcgctcatga ggctgtgggc acgcgagggc ccctaccttc
     1021 agcgcgccgc tgtcgcgggg ttgtgcgagc cggcgatcct caaaaggaat gaggatgcgg
     1081 tggccgtgct ggtgatcctg gacggaatca ccaagtcggt tgccttggct tcagctgccg
     1141 atcgccgcga cgagggattc gagggtttgc gcaaggcact gggttatgga tggagcatcg
     1201 ccgccgccgc agcgccgcgg aacgcgaagc cctacctgga gaagtggctc cgctcgaccg
     1261 acagggacgt cgcatggatc atgagaagca acatgaggaa ggcgcggatg gacgggctgc
     1321 gtgaacagct tgtctcttcc ccgccggtca ggacgcgtgg caaacatctc gcctgaggcg
     1381 atggcggcgc tcgagtccgg ccgcctcgcc cacatcgtga cgctgaatcc cgacaacagc
     1441 ccgcagatgt cgtgcgtctg gatcggcgtg gaggatgacg agattgtcat cggctcgctg
     1501 ccgcgcaaca ggaaggtgca gaacatcgag cgcgacgcgc gtgtggtgct ttcgatcgag
     1561 accgggcgca cgggcaacca tgggctggcc gagtacctgg tggtgcacgg tcgggctcgc
     1621 gtgacgcgag gcggtgcgcc agagctgctg cagcggcttg ctcacgtcta tatgggccct
     1681 gacgtcaagt tcccgccgat gcccaaccca ccgcccggct acgtgatcag gatcgaaccg
     1741 cgccgcttga gcggcaacgg cccgtggagc gattgagcta gacgcgctcc aggatcacga
     1801 tcgggatctc acgctcagtg agcttctgct gaggccccag gtattcgatc agcttggcga
     1861 tctccgggcg ctcctccggt gacgcgacgc gggtccggac cttgaatttc tcgctgccca
     1921 cctccacagt cgcgaccggg tccttctcca ggttggcgaa ccattgcgga gcgttcggcg
     1981 cgccgttgtc cgaggcgatg attgcgaggt cgtctccata cggccggtag ccgacgaccg
     2041 ccgttcgttg ccggcccgat ttggcccccg tcgtggtcag caggagaagc tgtctccccg
     2101 ccatctggcc actcatctga ccttggttgg cgcggaattc ctcgatcaac ttcgcattga
     2161 atgccctcat atcgttcgga atttgcgggc gctgttggtt cctcgacatg tcttaacagt
     2221 aaaacgcctt tgcgagtaag gacaccgggc ctcccgtttc cttccttagg aggtcaagag
     2281 aattgacaca gacactgatc gttgtactca tcgttgcggt cgtggtcatc ctcgcgatcg
     2341 ccctcatcgt cgcgttgacg cggcggtcat cccggcttcg tccgctgccg gctgagtcgc
     2401 gcgatcgttt tgctcacacg tggagcaacc tcgagagccg cttcatcgag gatccgcgcg
     2461 ccgcggtgca ggaagccgac cgtgtcgcgg tgctgatcct gagtgagcgc ggagccacga
     2521 tgcccgatgg gcgagacgtg ccgaagggcc tggaacacgc gcgcgcggcc gcctcgagcg
     2581 atgagggcag acagggtacc gaaggcatgc gcaaggcgat ggtgcactac aagcaaatcg
     2641 tcgatgacgc gatcggaaca gcgaaggaaa cgcgggacgc ttcccgc
//
LOCUS       DASPZD010000255         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_223644, whole genome shotgun sequence.
ACCESSION   DASPZD010000255 DASPZD010000000
VERSION     DASPZD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223644"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VEM33_04955"
     CDS             <1..491
                     /locus_tag="VEM33_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYS51204.1"
                     /translation="VAKYLGAKVIAAAGTDERVKAAVALGAGAGINYRTQNLTGEVMR
                     ITEKRGVDVVFENIGDPDLFPGAFAALARGGRLITAGGHGGGTVPLDVKQLYLNQNTI
                     IGSFGRIMPADVELGLRAAAEGRYRVLIDRILPLAEAAKAHRLVDTRAGIGKVILQPA
                     LS"
     gene            488..1633
                     /locus_tag="VEM33_04960"
     CDS             488..1633
                     /locus_tag="VEM33_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HYS51205.1"
                     /translation="MKRSSERFLTTHTGSLPRPEDLVRMMYAKEEGVPVDPRALGARI
                     RSAVEEIVQKQAAAGVDVVNDGEMSKPGYATYVKDRLDGFGGTGNTFVYQDLVEFPNL
                     AKRVFGDPGRSRRKTPACNAPIGVRDPKAARDDVDNLKAAASRVKTEETFMSAASPGV
                     IGLFFRNDHYPSQEAYLFAIADAMRHEYETVAKAGIVLQIDCPDLAMGRHIQYADLSL
                     AEFKKKARLHIEALNHATANIAPDRLRIHLCWGNYEGPHHCDVPLADIIDLVFSARPD
                     AISFEAANPRHAHEWKLFETVKLPEGKVLIPGVLESKTNFIEHPELIAQRIGRYAKLV
                     GRENVIAGSDCGYGTWVGQAAVDSDVVWAKMAAMAEGARIATKEFWR"
     gene            1636..1917
                     /locus_tag="VEM33_04965"
     CDS             1636..1917
                     /locus_tag="VEM33_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51206.1"
                     /translation="MPLKRFLGIVEREKKFLGEELTRTQKIMPLLMKPRNGERWTPED
                     KRELVEHLKRLSGMGPYVALLLLPGGLALLPLLAWWLDRRRDRRPPPPA"
     gene            complement(1931..2140)
                     /locus_tag="VEM33_04970"
     CDS             complement(1931..2140)
                     /locus_tag="VEM33_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51207.1"
                     /translation="MAGRALPVGAPYLRVFQLVGATAFIAYAAALWPTSIWYHRAWGT
                     TIRSTIDGLVYALLTAGTFGWLWPR"
     gene            complement(2141..2494)
                     /locus_tag="VEM33_04975"
     CDS             complement(2141..2494)
                     /locus_tag="VEM33_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51208.1"
                     /translation="MTGLSALWLPILLSSVIVFVASTVIHMALPWWHKSDFTKVPNED
                     RLRDALRPLAVAPGDYMVPRPSNMQEMRSPEFSEKMKKGPVMIFTMIPNGPPSMGKPL
                     VLWFLYCAVVGLFAA"
BASE COUNT          480 a          841 c          850 g          383 t
ORIGIN      
        1 aggttgcgaa atacctgggc gcgaaagtca tcgccgcggc aggcaccgac gagcgcgtca
       61 aggcggccgt cgcgctcggc gccggcgccg ggatcaacta ccgcacgcag aacctgaccg
      121 gggaggtgat gcgcatcacc gagaagcgcg gcgtcgacgt ggtgttcgag aacatcggcg
      181 accccgatct ctttcccgga gccttcgccg cgctcgcgcg gggcggccgc ctcatcaccg
      241 cgggcggcca cggcggcggc accgtgccgc tcgacgtgaa gcagctctat ctgaaccaga
      301 acacgatcat cggctcgttc gggcggatca tgcccgcgga cgtggagctc ggcctgcgtg
      361 cagcggccga aggccgctac cgcgtgctca tcgaccgcat cctgccgctc gccgaagcgg
      421 cgaaggcgca ccgcctcgtc gacacgcgcg ccgggatagg caaagtcatc ctgcagcccg
      481 ccttgtcgtg aagcgcagca gcgaacgctt cctcactacc cacaccggca gcctgccgcg
      541 gccggaggac ttggtgcgca tgatgtacgc caaggaggag ggcgtgccgg tcgatccgcg
      601 agcgctcggc gcgcgcatcc gctccgccgt ggaggagatc gtgcaaaagc aggccgcagc
      661 gggcgtcgac gtcgtcaacg acggcgagat gtcgaagccg ggctacgcca cctacgtcaa
      721 ggaccgcctc gacggattcg gcggcacggg caacaccttc gtataccagg acctcgtcga
      781 atttccgaac ctcgccaagc gcgtgttcgg cgatcccggc cgctcgcgcc gtaaaacccc
      841 ggcgtgcaat gcgccgatcg gcgtgcgcga cccgaaggcg gcccgggacg acgtcgacaa
      901 cttgaaagcg gccgcgtcca gagtgaagac cgaggaaacg ttcatgagcg cggcctctcc
      961 cggcgtaatc gggctcttct tcaggaacga ccactatccg agccaggagg cctacctttt
     1021 cgcgatcgcg gatgcgatgc gccacgagta cgagaccgtg gcgaaggccg gaatcgtcct
     1081 gcagatcgat tgcccggacc tcgccatggg tcggcacatc cagtacgcgg acctttcgct
     1141 cgccgagttc aagaagaagg cgcggctgca tatcgaggcg ctcaaccacg ctactgcgaa
     1201 catcgcgccc gaccggctgc ggatacacct gtgctggggc aactacgagg gcccgcatca
     1261 ctgcgacgtg ccgctcgccg atatcatcga tctcgtcttc agcgcgcggc cggacgcaat
     1321 ttccttcgag gccgccaacc cgcgccacgc gcacgagtgg aagttgttcg agaccgtcaa
     1381 gcttcccgaa ggcaaggtat tgatccccgg cgtgctcgaa tcgaagacca atttcatcga
     1441 gcacccggag ctgatcgccc agcgcatcgg ccggtacgcg aagctcgtcg gacgcgagaa
     1501 cgtcatcgcc ggaagcgatt gcgggtacgg aacctgggtc ggccaggcgg cggtggactc
     1561 ggacgtggtg tgggcgaaga tggcggcgat ggccgagggt gcgaggatcg ccacgaaaga
     1621 attctggcgc taggcatgcc ccttaagcgc ttcctcggga tagtggagcg tgaaaagaag
     1681 ttcctcgggg aggagctcac gcgcacgcag aaaatcatgc cgctgctgat gaagccgcgc
     1741 aacggcgagc gctggacgcc ggaggacaag cgcgagctcg tcgagcacct gaagcgcctc
     1801 tcgggaatgg gtccctacgt cgcgctcctc ctccttccgg gaggactggc gctgctgccg
     1861 ctgctcgcgt ggtggctgga ccggcgccgc gaccgccggc cgccgccgcc ggcttgaacc
     1921 cgggcgcgcg ctagcgcggc cagagccaac cgaaggtgcc cgcggtcagg agagcgtaga
     1981 ccaatccgtc gatagtcgac ctgatcgtgg tgccccacgc gcggtggtac cagatcgacg
     2041 tcggccagag cgcggcggcg tacgcgatga acgctgtggc accgaccagc tggaaaacgc
     2101 gcaggtacgg tgctccgacc ggcagggcgc gtccggccac ttaagccgcg aacagcccga
     2161 ccacggcgca gtagagaaac cacaggacga ggggcttccc catcgacggg gggccgttcg
     2221 gtatcatcgt gaatatcatc acgggacctt ttttcatctt ttcggaaaac tcaggcgagc
     2281 gcatctcctg catgttcgag ggacgcggga ccatgtagtc gcccggcgcc acggcgagcg
     2341 ggcgcagcgc gtccctcagc ctgtcctcgt tcggcacctt ggtgaaatcg ctcttgtgcc
     2401 accagggcaa ggccatgtgg atcacggtgc tcgcgacgaa gacgatcacc gacgacagca
     2461 ggatcgggag ccatagtgcg gacagtccgg tcatgacagc ctcctctggt tgaaccgatt
     2521 actcgcggta cgaaggatcg atcgagtcga gctt
//
LOCUS       DASPZD010000256         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_2574, whole genome shotgun sequence.
ACCESSION   DASPZD010000256 DASPZD010000000
VERSION     DASPZD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..251)
                     /locus_tag="VEM33_04980"
     CDS             complement(6..251)
                     /locus_tag="VEM33_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51209.1"
                     /translation="MPKPGKKAKQAFWERGYLAHGYWLGKERLGQVTLGPKKEWDGIY
                     RWRAANHSGEAATLDEAKRAVEQVVLVGASQLRLFDK"
     gene            366..1901
                     /gene="ilvA"
                     /locus_tag="VEM33_04985"
     CDS             366..1901
                     /gene="ilvA"
                     /locus_tag="VEM33_04985"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HYS51210.1"
                     /translation="MKNDYLERILRARVYDVAVETPLEVAPNLSVRLGNRVLLKREDM
                     QPVFSFKCRGAYNMMANLPSAALARGVIASSAGNHAQGVALAAQKLGCAAVIVMPSTT
                     PKIKVDAVKARGAEVHLHGDTYDEAHVEAKRLGRARRLGFVHPYEDPDVIAGQGTIGM
                     EILRQTAHPIHAVFVPIGGGGLVSGIASYIKRLRPAIKVVGVEPVDADAMARSLKAGR
                     RVVLDHVGLFADGVAVKQVGKETFRLCRELLDGVILVDTDQICAAIKDVFEDTRTILE
                     PAGALAIAGAKAYVEKSGVRGKKLVAVASGANTNFDRLRFVAERAEMGDRRETILAVT
                     IAERPGSFKKFCATLGARNITEFNYRIADPKDAHVFVGVEVHSRDESAKIVKVLEREG
                     LKTLDLSDDEMAKLHVRHMVGGHAPPAIRRDARRAADELLYRFEFPERPGALMRFLSG
                     MRTDWNISLFHYRNQGSDYGRVLVGMQVPRHEMREFRRFLAELGYPYVDESSNPAYRL
                     FLG"
     gene            complement(1904..2578)
                     /locus_tag="VEM33_04990"
     CDS             complement(1904..2578)
                     /locus_tag="VEM33_04990"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HYS51211.1"
                     /translation="MTPSELSAWRKNERTRLLRERETLTPETLAELRSRIDHHLERAF
                     PDLVHGILAFCWPYRNEYDARHLAATLRRQGATTALPAVVAPKAPLVFREWKPGVELA
                     VGPLGIPYPVGSPEVNPDHVLLPMLGWDADGYRLGYGGGFFDRTLAALARRPRVVGIA
                     YEQAFLKTIHPQPHDIPVDFVVTERGVYRREPKGLKFLDDPKGYSSPPCYAGEIAPGY
                     FGEPKT"
     gene            2644..>4124
                     /locus_tag="VEM33_04995"
     CDS             2644..>4124
                     /locus_tag="VEM33_04995"
                     /inference="COORDINATES: protein motif:HMM:NF026068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51212.1"
                     /translation="MIRALLRPACLAVALAAAAPACAAKLDAQDAAFLAARDAFEANH
                     RARLAALAPKLEGHLLEPYVEYWQLFFRLPGASSADVRDFLSRYQGTALAEQLRVDWL
                     KVLGRSGRWELFQAEYPALSGDDPEVTCYMLLARWKREDAVVLGDFRPFWSAPRELPE
                     GCLTLARSIHKAGQIGSREAWERFRVLVGAGLMGAAKRTMEFLPRGEAIDAKRLGTVI
                     RAPVKFLRNPHVDLAKVTDRELVIAALTLAADADVRTAAALWSGGLSDGFSVEDRRYV
                     WLMLAMNGALRHVPDALDWFGDPGALTDEQLGWRARIAMRTEKWREVRNSIDRMSPLA
                     KNEPVWIYWYGRAERKLGSPLEAEGYFERIAGEHSFYGRLAAEELGVSLRIPARAPLP
                     TESEIAEAAAIPGLARALALYRLDMRTEATKEWLWTIRGMDDRKLLAAAELARRNEAW
                     DRAIGTADRTVLAHNFSVRYLAPYREVLAENARSRDLEEPWVLG"
BASE COUNT          695 a         1352 c         1363 g          714 t
ORIGIN      
        1 tccttttact tgtcgaacaa acgcagctgg ctcgccccga cgaggacgac ctgttcgact
       61 gcgcgcttgg cttcgtccag cgtggcagct tcgccggaat ggttagccgc ccgccagcgg
      121 tagatcccgt cccattcctt cttcgggccg agcgtgacct gccccagccg ctcttttccg
      181 agccagtatc cgtgcgcgag gtagccgcgc tcccagaacg cctgtttcgc ctttttcccg
      241 ggcttcggca tcggctcctc ccgagccggc attttaccca cggccggggc gttcgtgtac
      301 agtatcgccc acccctcaaa ggggggttgc acttgacccc ccctttaggg ggtaagagaa
      361 acgcgatgaa aaacgactat ctggagcgga ttctccgggc gcgcgtgtac gacgtggcgg
      421 tggagacgcc tctggaagtc gcacccaatc tctcggtgcg cctcggcaac cgggtgctct
      481 tgaagcgtga ggacatgcag ccggtgttct ccttcaagtg ccgcggcgcc tacaacatga
      541 tggcgaatct tccctcggcg gcgcttgccc gcggagtcat cgcttcttcg gccggcaatc
      601 atgctcaagg cgtggccctt gcggcccaga agctcggctg cgcggcggtg atcgtgatgc
      661 cctccacaac ccccaagatc aaggtcgacg cggtcaaggc ccgcggagcg gaagtgcact
      721 tgcacggcga tacctacgac gaagcgcacg tcgaggcgaa gcgcctggga agggcacgca
      781 ggctcggctt cgttcatccc tacgaggatc cggacgtgat cgcgggccag ggcacgatcg
      841 gaatggaaat cctgcgtcag accgcgcacc cgatccacgc cgtattcgtg ccgatcggcg
      901 gcggcgggct ggtttccggc atcgcttcct acatcaagcg gctgcgcccg gcgatcaagg
      961 tcgtcggcgt cgaaccggtg gacgccgacg ccatggcgcg ctcgctcaag gccggacggc
     1021 gcgtcgtgct ggatcacgtg ggtcttttcg ccgacggggt cgcggtgaag caggtcggca
     1081 aagagacgtt tcgcctgtgc cgggaactcc tcgacggcgt gatcctcgtc gacaccgacc
     1141 agatctgcgc ggcgatcaag gacgtgttcg aggacacgcg cacgatcctc gagcccgccg
     1201 gcgcgctggc gatcgccggc gccaaggcct acgtcgaaaa atccggggtg cgcggaaaga
     1261 agctcgtcgc cgtcgcctcg ggcgcgaaca cgaatttcga ccggctgcgc tttgtcgccg
     1321 agcgcgccga gatgggcgat cggcgcgaga ccatcctcgc ggtcaccatc gccgagcgtc
     1381 cgggcagctt caagaagttc tgcgcaacgc tcggagcgcg taacatcacc gagttcaact
     1441 accgcatcgc cgatcccaag gatgcgcacg tattcgtcgg cgtcgaggtg cacagccgcg
     1501 acgagtccgc gaaaatcgtg aaagtcctcg agcgcgaggg tctgaagacg ctcgacctct
     1561 ccgacgacga gatggccaag ctccacgtgc gtcacatggt cggcggccat gcgccgccgg
     1621 cgatccggag ggacgcgcgc cgcgccgccg acgagttgct ctaccgcttc gagtttccgg
     1681 agcggcccgg ggcgctcatg cgctttttgt cgggcatgcg caccgactgg aacatcagcc
     1741 tgttccacta tcgcaatcag gggagcgact acggccgggt gctggtcggc atgcaggtgc
     1801 cgcgccacga gatgcgcgaa ttccggcgtt ttctcgccga gctcggctat ccgtacgtgg
     1861 acgaaagcag caatccggct taccggctgt ttctcggttg agactaggtc ttcggttctc
     1921 cgaagtatcc gggcgcgatc tctcccgcgt agcacggcgg cgaagagtag cctttcgggt
     1981 cgtccaggaa cttgagcccc ttcggttcgc ggcggtatac gccgcgctcg gtgacgacga
     2041 aatccacggg aatgtcgtgc ggctgaggat gtatggtctt caggaaggcc tgctcgtagg
     2101 cgatcccgac gacgcgcggc cgtctggcga gcgccgcgag ggtacggtcg aaaaagccgc
     2161 cgccgtagcc gagccgataa ccgtccgcat cccaccccag catgggtagc agcacgtgat
     2221 ccgggttgac ctcgggcgaa ccgacagggt acggaatgcc gagtggcccg acggccagtt
     2281 ccactccggg tttccattcg cgaaaaacga gcggggcctt gggggctacg acagccggca
     2341 gcgcggtcgt cgccccctgc cgccgcagcg tcgcagccag atggcgtgcg tcgtattcgt
     2401 tccggtacgg ccagcaaaac gccaatattc cgtgcaccag atccgggaac gcacgttcaa
     2461 ggtggtggtc gatccgcgag cgcagctccg cgagcgtctc cggggtgagc gtttcgcgtt
     2521 cccgcaaaag cctggtgcgc tcgttcttgc gccaggcgga aagttcggaa ggggtcatgt
     2581 acggaggccc ttaatctggc acgaaaaatg cgtgtagtat tctatcaaag gaggaagtgg
     2641 accttgatac gagcgctgct ccgtccggcc tgtcttgccg tcgccctcgc agcggcggcg
     2701 cccgcgtgcg cggcgaagct cgatgcgcag gacgcggcgt tcctcgccgc ccgcgatgcg
     2761 ttcgaagcga accaccgggc caggctcgca gcgctcgccc cgaagctcga aggccacctg
     2821 ctcgagccct atgtcgagta ctggcagctc ttcttcaggc ttcccggcgc atcgtccgca
     2881 gacgttcgcg acttcctttc ccgatatcaa ggtaccgcgc tcgcagagca gctgcgcgtc
     2941 gattggctca aggtgctcgg caggagcgga cgatgggagt tgttccaagc ggaatatccg
     3001 gcgctctccg gcgacgatcc cgaggtgacc tgctacatgc tgctcgcgcg ctggaagcgc
     3061 gaggatgccg tggtgctcgg cgatttcagg cccttctgga gcgctccgcg tgaacttccc
     3121 gaaggctgcc tcactctggc ccggtcgatc cacaaggccg gccaaatcgg ctcgcgcgaa
     3181 gcatgggagc ggtttcgcgt cctggtcggc gccgggctca tgggcgcggc caagcgcacc
     3241 atggagtttc tgccgcgcgg cgaagccatc gacgcgaaac gactcgggac cgtcatccgg
     3301 gcgccggtca agtttctcag gaatccgcac gtcgacctgg cgaaggtgac cgatcgagag
     3361 ctggtcatcg ccgccctcac gctggctgcc gacgcggacg tgcggaccgc cgcggcgctc
     3421 tggagcggcg gcctgagcga tggcttttcc gtcgaagacc gcaggtacgt ctggctgatg
     3481 ctggccatga acggcgcgct ccggcacgtg cctgacgcgc tcgactggtt cggtgatccg
     3541 ggagcgctca ccgacgagca gctcggctgg cgtgcgcgaa tcgcgatgag gaccgaaaag
     3601 tggcgcgagg tcagaaactc catcgaccgg atgtctccgc tcgccaagaa cgagccggtc
     3661 tggatctact ggtacggccg cgccgagcgc aagctcggca gcccgctgga agccgaaggt
     3721 tatttcgagc gcatcgccgg agagcacagt ttctacgggc gccttgccgc cgaggaactc
     3781 ggcgtgagcc tgcggattcc ggcgcgggct cctctgccca ctgaaagcga aatcgccgag
     3841 gctgcagcca ttcccgggct cgcgcgtgcg ctcgccctct atcgcctgga catgcggacc
     3901 gaggcgacga aggagtggct ctggaccatc cgtggcatgg acgatcgcaa gctgctcgcg
     3961 gcggccgagc tcgcccggcg caacgaagcc tgggacaggg ccatcggcac cgcggacaga
     4021 acggtactcg cccacaactt ttcggtccgc taccttgcgc cttatcgcga ggtgctcgcc
     4081 gagaacgcgc gctcgcgcga tctcgaggag ccctgggtgc tggg
//
LOCUS       DASPZD010000257         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_948490, whole genome shotgun sequence.
ACCESSION   DASPZD010000257 DASPZD010000000
VERSION     DASPZD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948490"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..2184)
                     /locus_tag="VEM33_05000"
     CDS             complement(37..2184)
                     /locus_tag="VEM33_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYS51213.1"
                     /translation="MSRRQLLVAALLIGLIAAFFALDLGQYLRLEYFRSQQSAIEEFR
                     RANPLIASFIFFGIYVVVTSLSLPFAAVMSLAIGAVFGLLWGTLIVSFASSVGATLAF
                     LSSRFLFREAVRNRFGGRLRAIDAGLEKEGAFYLFALRLVPAVPYIVVNLVMGVTPIR
                     TWTFYWVGQIGMLPATIVYVNAGTQLATVKSLSGILSPTLIGSLVLIGIFPLIAKHAV
                     EVFKARRRLARWPKPARFDRNLVVIGAGSAGLVAAYIAAAVKAKVTLIEKDRMGGDCL
                     NTGCVPSKALIKSARLVAQAKRPREFGFKAMQVDFDFADVMERVQRVVRTVEPHDSAE
                     RYTKLGVECLKGEAKITSPWTVEVKSETGRRALTTRAIVIAAGARPFVPPIPGLAEAG
                     CLTSDSVWSLRALPARLVVLGGGPIGCELAQCFARFGSRVTQLEMLPRILIGEDPEIS
                     EMVARQFREDRIDVRTGHKATRVLREGGEKLLSCEHDGKEVRIAFDELLCAVGRIANT
                     EGYGLEELGIELSRARTVSTNEYLQTLYPNIYACGDVAGPYQFTHTASHQAWYAAVNA
                     LFGGVKRFRADYSVIPWATFTDPEVARVGLNETEAKEKNVAYDVTNYPLEDLDRAIAD
                     GETAGVVKVLTVPGRDRILGATIAGEHAADLIVEFITAMKHGIGLNKILGTIHIYPTL
                     AEGNKYAAGSWKRAHAPLGLLKWAEKFHAWRRG"
     gene            complement(2198..>2645)
                     /locus_tag="VEM33_05005"
     CDS             complement(2198..>2645)
                     /locus_tag="VEM33_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51214.1"
                     /translation="QFSQEALRERPERAGALLAGSGVDVFACTHTCLPVIQEFDAPHG
                     KALIVNNGAAGMPNFRDTRFGLATRIAGTPSAQALYGARLGAAVVEAVPVRYDHEAWL
                     AEFDRAWPAGSPAALSYRKRIASGPGYEVEQAVRTGARTGAFSRAA"
BASE COUNT          466 a          882 c          871 g          426 t
ORIGIN      
        1 ccgcctcgtt cagcgccggg acgatgatcg aaatcttcaa ccgcgcctcc acgcgtggaa
       61 cttctccgcc cacttgagca gccccagcgg ggcgtgcgct ctcttccagc tgcccgccgc
      121 gtacttgttg ccttcggcaa gcgtgggata gatgtggatc gtgccgagga tcttgttcag
      181 tccgatgccg tgcttcatcg ccgtgatgaa ttcgacgatg agatcggccg cgtgttcccc
      241 ggcgatcgtt gcgccgagga tcctgtccct gccgggcacg gtgaggacct tcaccacccc
      301 tgccgtctcg ccgtcggcaa tcgcgcggtc gaggtcctcg agagggtaat tggtgacgtc
      361 gtaagcgacg ttcttctcct tggcctcggt ttcgttcagc cctacgcggg cgacctcggg
      421 atcggtaaac gtcgcccagg ggatcacgga gtagtcggcg cgaaatctct tcactccgcc
      481 gaagagcgcg ttcaccgccg cgtaccaggc ctggtgcgag gcggtatgcg taaactgata
      541 cgggccagcg acgtcgccgc aggcgtagat gttggggtaa agggtctgca ggtattcgtt
      601 cgtgctgacg gtgcgcgcac ggctcaattc gatgccgagc tcctccaggc cgtacccctc
      661 ggtattggcg atgcgcccga ccgcgcacag cagctcgtcg aacgcgatgc ggacctcctt
      721 gccgtcgtgc tcgcacgaga gcagcttctc gccgccctcc cggagcacgc gcgtcgcctt
      781 gtgaccggtg cggacgtcga tgcggtcctc gcggaactgc cgcgcgacca tttcggagat
      841 ctccgggtct tccccgatga ggatgcgcgg aagcatctcg agctgcgtca cgcgcgaacc
      901 gaacctcgcg aagcactgcg cgagctcgca gccgatcggc cctcccccga ggacgaccag
      961 ccgcgccggc agcgctctca agctccacac cgagtccgag gtcaggcagc cggcttcggc
     1021 gagccccggt atcggcggca cgaaagggcg cgcgcccgcg gcgatgacga tcgcccgggt
     1081 cgtgagcgcg cgcctgcccg tttcgctctt cacctccacc gtccaggggg acgtgatttt
     1141 cgcctcgccc ttgaggcact cgacgccgag cttggtatag cgctcggccg agtcgtgcgg
     1201 ttccacggtt ctcaccacgc gctgcacgcg ctccatgacg tctgcgaagt cgaagtcgac
     1261 ctgcatggct ttgaagccga attcgcgggg gcgcttcgcc tgcgcgacga gccgcgccga
     1321 cttgatcagg gccttggagg gcacgcagcc cgtgttgagg cagtccccgc ccatcctgtc
     1381 tttctcgatc agggtcactt tcgccttgac cgcggccgcg atgtaggccg cgaccaggcc
     1441 ggcggaaccc gcgccgatca cgacgaggtt gcggtcgaat cgagccggct tcggccatct
     1501 tgccagccgc cggcgcgcct tgaagacctc gacggcgtgc ttggcgatga gcggaaagat
     1561 gccgatcagc acgagcgagc cgatcaacgt cggggagaga atccccgaga gcgacttgac
     1621 ggtagccagc tgggtgcccg cgttgacgta aacgatcgtg gcgggaagca tgccgatctg
     1681 gcccacccag tagaaggtcc aggtcctgat gggcgtgacg cccatcacca gattcacgac
     1741 gatataaggc accgcgggca cgagccgcag ggcgaagaga tagaacgcgc cctccttctc
     1801 caggcccgcg tcgatcgcgc gaagacgacc accgaaacgg ttccggaccg cctcgcggaa
     1861 gagaaacctg gaggagagaa acgcaagcgt tgcgccgacg cttgaggcga acgatacgat
     1921 cagcgtgccc cacaggaggc cgaagaccgc gccgattgcg agagacatga ccgccgcgaa
     1981 cgggagcgaa agcgaggtga caacaacgta gattccgaaa aaaatgaaac tcgcgataag
     2041 cggattcgct cgccggaatt cctcgatcgc cgactgctgg gacctgaagt attcgagccg
     2101 gagatactgg ccgagatcga gcgcgaaaaa cgcggcgatc aggccgatga gaagggccgc
     2161 gacgagcagc tggcgacgcg acatggtccg cgccgcttca ggctgcgcgc gagaaagcgc
     2221 ccgtccgagc gcccgtccgc accgcctgtt ccacctcgta acccggaccg gaagcgatgc
     2281 gcttgcgata agagagcgcg gcgggtgagc ctgcgggcca ggcgcggtcg aattccgcca
     2341 gccacgcttc gtggtcatag cgcacgggca cggcttcgac gaccgccgct ccgagccgcg
     2401 cgccgtagag cgcctgcgcc gagggcgtgc ccgcgatgcg cgtcgcgagg ccgaaacgcg
     2461 tgtcgcggaa attcggcatt ccggcggcgc cgttgttcac gatgagcgcc ttcccgtgcg
     2521 gcgcgtcgaa ttcctgaatc accggcaggc aggtgtgcgt acacgcgaag acgtcgacgc
     2581 cggatccggc gagcagcgca cccgcgcgtt cgggacgctc gcgcagcgcc tcctgcgaga
     2641 actgc
//
LOCUS       DASPZD010000258         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_14080, whole genome shotgun sequence.
ACCESSION   DASPZD010000258 DASPZD010000000
VERSION     DASPZD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14080"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1134
                     /locus_tag="VEM33_05010"
     CDS             301..1134
                     /locus_tag="VEM33_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HYS51215.1"
                     /translation="MMVMTSVSLRLSLLLQLLALVPLLANAQAPSAGAAPSPIAPTGY
                     SNWDAQFLFGSRFREPGIADDVSKGILTIENAAGWSWGSSYFFVDVLRSDGADSHATE
                     VYSEWYPSASLSKLSGRDFAFGPVRDVSATLGINAGTKSTGAAPLVLLPGVTLDLKLP
                     GFAFFSLGTYFYIDKGRIGGASNGCRDTTYQVTPSWALPFRIGSVQLSFDGFIDFIGS
                     HGQCESQIISQPQLKVDLASFGAKPGVFQIGIEWQYWHNKFGIEGLNESFPQALFVWK
                     Y"
     gene            complement(1203..1613)
                     /locus_tag="VEM33_05015"
     CDS             complement(1203..1613)
                     /locus_tag="VEM33_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HYS51216.1"
                     /translation="MPIRNVMFIVAFTLAATSAGAQSNLEKLDKAYREGPCSNDLKSY
                     CARVTPGDARIADCLAQHFREIQPACRGLITSARNRFDAMAAACKGDAEKLCKGVAYR
                     EGRVLSCLKGREASLSAACAVEFKRIGSDHAATQ"
     gene            complement(1690..2706)
                     /locus_tag="VEM33_05020"
     CDS             complement(1690..2706)
                     /locus_tag="VEM33_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HYS51217.1"
                     /translation="MKFALGAWLLVLASSPAVAQVVLNASSSAPPAHALTAGMLVPFC
                     ADIESVTSARVKCNILPRAPVGAAQTLDGIKDGLMDLSFITHGYTPGRFALTEAAEFP
                     FMGDTAEITSVAFQRIHERALARTKEHGDLVLLSVFTHGPGQIYNTRRPVRAIADLEG
                     LKIRVGGGIGNDVARSLGAVPMLKSATESYELLSSGIADGVFFPKDSPMSFKLIPLIK
                     HVTYVPGGLYNVSFAWVANPEKWSRISAADRKLIEPLLGEALARRSGRAWDAADAGGE
                     KAVREAKIPVVVAGAQLIAEIRARAEPLEREWIEKKAKPKGVDGDAVLKALRAEIAML
                     QRAR"
     gene            complement(2711..>3336)
                     /locus_tag="VEM33_05025"
     CDS             complement(2711..>3336)
                     /locus_tag="VEM33_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HYS51218.1"
                     /translation="LSILAFFIVERGWTAASFAAAGIALLFLYDAAPVPLKSIGLGEP
                     AVFLVWGPLMIGGGYAMIAGEISPAAFWASIPYGLGVMSILVGKHIDQMDFDRGKRIR
                     TLPVLLGERTARALNVATVALIYAAAAALIVLGLLTPFAALIVVALPRAWRAVKLMSR
                     PRPPAAPAGYVGWPLWYHRACLVHNRLFGWAYIVGLAGGAAWPNLRF"
BASE COUNT          568 a         1117 c         1074 g          577 t
ORIGIN      
        1 cgtcgcatag gggcacgagc atgttcgccg tgatcggatg cgcgggcggc acccacgagc
       61 tcgcgttgag caccacttgt gcgccggccg ccgaagacag cgcgaccagc acgagcccgc
      121 ccaggatttt cttcatggct ccctccaaca tgaattatgt tgcatgcgcc tcggaacgac
      181 cgggtcaaga atagcaggag accgcggcta tttcttcagt ggatagagca gccccctcct
      241 aaggggccca tgaggctccg tccgcgcacc tcgaatcgga gcgagtctag tggtaaggtg
      301 atgatggtca tgacatcggt atcactacgg ctgtccctgc tattgcagct cctggccctt
      361 gtgccgctac tggcaaatgc gcaggctccg tcggccgggg cggcgccctc gcccatcgct
      421 cccaccggct attcgaactg ggatgcgcag ttcctctttg ggagtagatt ccgcgagccc
      481 ggtatcgcgg acgatgtatc caagggtatt ctcaccatcg agaacgccgc cggctggagc
      541 tggggcagca gctatttttt cgtggatgtc ctgcgatcgg atggtgccga cagccatgca
      601 accgaggtct actccgaatg gtatccctcc gccagcctga gcaaactcag cggcagggac
      661 ttcgccttcg gccctgtacg ggatgtttcc gcgaccctgg gcattaatgc cggcacgaag
      721 agcaccggcg ccgcgccgct ggtactgctg ccgggcgtga cgctggacct gaagcttccc
      781 ggattcgctt tcttctcgct cggcacgtac ttctacatcg acaaggggcg catcggcggg
      841 gcgagcaacg gctgccgcga cacgacctac caggttacgc cgtcgtgggc gctgccgttc
      901 cgcatcggca gcgtccagtt gtcattcgac ggcttcatcg atttcatcgg cagccacggc
      961 caatgcgaat cccagattat ttcccagccc cagctcaagg tcgacctggc gagcttcggc
     1021 gccaagccag gcgtcttcca gatcgggatc gaatggcagt actggcacaa caagttcggc
     1081 atcgagggcc tgaatgagag cttcccccag gccctgttcg tgtggaaata ctaaggaaac
     1141 tctgaattat tcctcggctc aatgcctacg cattagagca gaggaagatc ccgtgggccg
     1201 tcctattgcg tagccgcgtg gtcgctgcct atgcgcttga actccacggc gcacgccgcg
     1261 ctgaggctgg cctcccggcc cttgaggcag ctcaggacgc gtccttccct gtaggcaacg
     1321 cccttgcaga gcttctccgc gtcgcctttg cacgccgcgg ccatcgcgtc aaacctgttg
     1381 cgggcggacg tgatcaggcc ccggcatgcg ggctggatct cccggaaatg ctgcgccagg
     1441 cagtcggcga tccgggcgtc ccccggcgtg acgcgggcgc agtagctctt gaggtcgttc
     1501 gagcaggggc cttccctgta ggccttgtcg agcttctcca gattcgactg cgctccggcg
     1561 gacgttgcgg caagcgtgaa tgccacaatg aacatcacgt ttctgatcgg catggttccc
     1621 cctttcaagg gatagcagaa gggatagtaa aatcaattcg ccgctgaagc caaggtggct
     1681 cgccggcatc tatctggccc gctgcagcat cgcgatctcg gcgcgcagcg ccttgagcac
     1741 ggcgtccccg tccaccccct tcggcttcgc cttcttctcg atccactcgc gctccagcgg
     1801 ctcggccctc gccctgatct cggcgatcag ctgcgcgccg gcgacgacga ccgggatctt
     1861 cgcctcgcgc actgctttct cgcctccggc gtcggccgcg tcccaggccc ggccggagcg
     1921 gcgcgcgagc gcctcgccga gcagcggctc gatcagcttg cggtcggccg cggagatccg
     1981 gctccatttc tccggattcg cgacccacgc gaagctcacg ttgtacaggc cgccgggcac
     2041 ataggtcacg tgcttgatga gcgggatcag cttgaacgac atgggcgaat ccttcgggaa
     2101 gaacacgccg tcggcaatgc ccgaggagag cagctcgtag ctctcggtcg cgctcttgag
     2161 catcggcacc gcgcccaggc tccttgcgac gtcgttgcct atccctccgc cgacgcggat
     2221 cttcagtccc tccagatccg cgatcgccct gacgggtcgg cgcgtgttgt agatctgtcc
     2281 cgggccgtgg gtgaacaccg aaaggagcac gagatccccg tgctccttcg tcctcgccag
     2341 cgcgcgctcg tggatgcgct ggaacgcgac ggaggtgatc tcggcggtat cgcccatgaa
     2401 ggggaattcc gcggcctcgg tgagcgcgaa gcggccgggc gtatacccgt gggtgatgaa
     2461 cgacaggtcc atcaggccgt ccttgattcc gtcgagagtc tgcgccgcgc ccacgggcgc
     2521 cctgggcagg atattgcatt tgaccctggc gctggtcacg ctttctatgt cggcgcagaa
     2581 cggcacgagc atgcccgcgg tgagggcatg cgcgggcggg gccgaggagc tcgcgttgag
     2641 cacgacctgc gcgacggccg gcgacgacgc aaggaccagg agccaggcgc cgagcgcgaa
     2701 cttcacggtt ctagaaccgc agattcggcc aggccgcgcc gcccgcgagc cccacgatgt
     2761 acgcccaccc gaacagccgg ttgtgcacga ggcaggcgcg gtggtaccac agcggccagc
     2821 cgacgtagcc cgcgggagcc gccggcggac gcgggcggct catgagcttc accgcccgcc
     2881 aggcgcgcgg cagcgcgacg acgatcagcg cggcgaaggg cgtgagcagg ccgaggacga
     2941 tcaacgccgc ggcggcggcg tagatcaggg cgacggtcgc gacgttgagc gcccgcgccg
     3001 tccgctcgcc gagcagcacc ggcagcgtgc ggatgcgctt gccgcggtca aagtccatct
     3061 ggtcgatgtg cttgccgacg aggatcgaca tcacgcccag cccgtaggga atcgatgccc
     3121 agaatgccgc gggcgaaatt tctccggcga tcatcgcgta gccgccgccg atcatgagcg
     3181 gcccccacac caggaacacc gccggctcgc ccaggccgat gcttttcagg ggcaccggcg
     3241 ccgcgtcgta gaggaacagc agcgcgatcc ccgcggcggc gaaggacgcg gccgtccagc
     3301 cgcgctcgac gatgaagaaa gcgaggatcg aaaggc
//
LOCUS       DASPZD010000259         3913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1466609, whole genome shotgun sequence.
ACCESSION   DASPZD010000259 DASPZD010000000
VERSION     DASPZD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466609"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEM33_05030"
     CDS             <1..249
                     /locus_tag="VEM33_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51219.1"
                     /translation="GIPGYEVTAWFGIMLPKGAPKDIVLRINGEVNKALVQADLKERL
                     LQQGAIATAWTPEQFGSFIHDEVVKWAKVVKASGAKVE"
     gene            complement(276..1454)
                     /locus_tag="VEM33_05035"
     CDS             complement(276..1454)
                     /locus_tag="VEM33_05035"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYS51220.1"
                     /translation="MTEAVIVSTARTPLCKSWRGAFNITHGATLGAHAVRHAVSRAGI
                     DSAEVEDVIMGCAHPEGATGGNIARQIALRAGLPVTTAGITVNRFCSSGLQAIAMASQ
                     RIMAGEGGIYAAGGVESISCVQNEMNKHMGREDWLTENKPELYWPMLQTAETVAKRYD
                     ISRERQDEYGARSQQRAAAALAAGKFGDELVPMKVVMGIADKETARMTTKEVTVSADE
                     GIRADTTYEGVSKIRPAIEGGCIAAGNASQFSDGASACIVMDGKAAAARKLEPLGIFR
                     GFAVAGCEPDEMGIGPVFAVPKLLKRAGLKVEDVGLWELNEAFAVQVIYCRDRLGIPD
                     DRLNVNGGAIAVGHPYGTSGARLAGHALIEGRRRRAKHVVVTMCIGGGMGAAGLFEVV
                     "
     gene            complement(1473..3563)
                     /locus_tag="VEM33_05040"
     CDS             complement(1473..3563)
                     /locus_tag="VEM33_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYS51221.1"
                     /translation="MGATIYELRGTVALITMDNPPVNGLGFDLRHDIVEGLARAEADA
                     NVKAVVLIGSARAFSGGADIREFGSPRASAEPSLNTVIRMLENAGKPVVAAIAGACMG
                     GGLELALGCHYRVAVKGAQIALPEVKLGLIPGAGGTQRLPRLIGAEAALNMIVSGNSV
                     PSEQLKDSGLFDEFFEKDLLPGAIAFADKVASGKRPLKRVRDLKASLANADGFFQFAR
                     NTVRAAAKNFPAPLKCVDAVAAAVTMPYEEGARFERESFLQLMQTPQSRALRHAFLGE
                     RAAAKIPDVPEDTPLRKVEKMAVVGAGTMGVGITMNFLSAGIPVTLLEMKQEALDRGL
                     AAIRRNYEASARKGKLTAQQLQERMALLEPTLSYDDLKDADMVIEAVFEDIAVKEAVF
                     RRLDEVMKPGAVLASNTSTLDLNRIAEFTRRPMDVVGTHFFSPANVMKLLEVVRGRKT
                     AKDVLATVMGLAKKIRKTAVVSGVCDGFIGNRMVEQYIRQALFLLEEGATPSQVDRAL
                     ETWGMAMGPFRMSDLAGNDIGWAIRKRRYRERPNVKYSKIADRLCEQGRFGQKTGLGW
                     YRYEAGRRDALPDSTVEQMIVDYRREIGAQHRAIGEDEIVHRLIFALVNEGSAILQDG
                     ISSRASDIDMVYLTGYGFPLHRGGPMLYADEVGLYNVVDSMKRFAANPHGDPAFWKPA
                     PLLAKLASEGKRFN"
BASE COUNT          670 a         1318 c         1200 g          725 t
ORIGIN      
        1 gggatcccgg gctacgaagt gacggcgtgg ttcggcatca tgctccccaa aggcgcgccg
       61 aaagacatcg tcctgcgcat caacggcgaa gtgaacaagg cgctcgttca ggccgacctg
      121 aaagaaaggc tcttgcagca gggcgcgatc gcaaccgcct ggaccccgga acagttcggc
      181 tccttcatcc acgacgaggt cgtgaaatgg gccaaggtgg taaaggcttc cggcgccaaa
      241 gtcgaataga cgcgccccgc acacttcttc ccgacttaaa cgacctcgaa cagccctgcg
      301 gcccccatcc cgccgccgat gcacatcgtc accacgacgt gcttcgcgcg ccggcgcctg
      361 ccttctatga gcgcgtgacc cgcgaggcgc gcgccggaag tcccgtaggg atggccaacc
      421 gcgatggctc cgccgttgac gttcagcctg tcgtcgggga tgcccagacg atcgcggcaa
      481 tagatcacct gaacggcgaa agcctcgttc agctcccaga gtccgacgtc ctccactttc
      541 aatcccgcgc gcttcaggag cttcggcacg gcgaacacgg ggccgatccc catctcgtcg
      601 ggctcgcagc ccgccacggc gaacccgcga aagatgccca ggggctcgag cttgcgggcc
      661 gccgccgcct tcccgtccat cacgatacag gcggaggcgc cgtcggagaa ctgactcgca
      721 ttgccggcgg cgatgcaccc gccttcgatc gccgggcgga ttttcgagac tccctcgtag
      781 gtggtatcgg cccggatgcc ttcatcggcg gagacggtga cctccttcgt cgtcatgcgc
      841 gcggtctcct tgtcggcgat gcccatgacg actttcatgg gcacgagctc gtcgccgaac
      901 ttgcccgcgg ccagcgcggc agcggctctt tgctggctgc gcgcgccgta ctcgtcctgg
      961 cgctcccgcg aaatgtcgta gcgcttggcg acggtctccg cagtttgcag catgggccaa
     1021 tagagctcgg gtttgttctc ggtgagccaa tcttcccgac ccatgtgctt gttcatctcg
     1081 ttctggacgc aggagatgga ctcgaccccg ccggccgcgt agatgccgcc ctcccccgcc
     1141 atgatgcgct gcgatgccat ggcgatcgct tgcaggcccg acgaacagaa gcggttcacc
     1201 gtgatcccgg cggtcgtgac gggcaggccg gcgcgcaacg cgatctggcg ggcgatgttc
     1261 ccgccggtcg cgccttcggg gtgggcgcag cccatgatca cgtcctccac ttccgccgag
     1321 tcgatccctg cgcgggagac cgcgtgccgg acggcgtgcg cgcccagggt cgcgccgtgg
     1381 gtgatgttaa aggcgccccg ccacgatttg caaaggggcg tgcgcgcggt ggaaacgatt
     1441 acggcttcgg tcattgacgc tctccaggac tcctagttga acctctttcc ttcgctcgcc
     1501 agcttcgcaa gcaggggcgc gggcttccag aaagccggat ccccgtgggg attggccgcg
     1561 aagcgcttca tgctgtcgac gacgttgtac aggcccacct cgtcggcgta gagcatcggc
     1621 ccgccacggt gcaggggaaa accgtacccg gtcaggtaga ccatgtcgat gtccgaggcg
     1681 cgcgatgaaa tgccgtcctg caggatcgcc gacccttcgt tgaccagcgc aaagatcaag
     1741 cgatgcacga tttcgtcctc gccgatcgcg cggtgctgcg ctccgatctc cctgcggtag
     1801 tcgacgatca tctgctcgac cgtggagtcc ggcagcgcgt cgcgacggcc ggcttcgtaa
     1861 cgataccaac ccagtccggt cttctggccg aagcgaccct gttcgcacaa gcggtcggcg
     1921 atcttcgagt acttgacgtt gggccgctcg cggtagcgcc gcttgcgaat ggcccagccg
     1981 atgtcgttgc ccgccagatc cgacatgcgg aaagggccca tcgccatgcc ccaggtttcc
     2041 agcgcgcggt ccacttgact cggcgtggca ccttcctcca gcaggaacaa tgcctggcgg
     2101 atgtactgct cgaccatgcg gttgccgatg aaaccatcgc atacgcccga gaccaccgcg
     2161 gtcttcctga tttttttcgc gaggcccatc accgtggcga ggacatcctt ggcggtcttc
     2221 cggccgcgca cgacttcgag gagcttcatc acgttggcag gactgaagaa gtgcgtgccg
     2281 acaacgtcca tgggccgccg ggtgaactcc gcgatcctgt tcaaatccag cgtggaggtg
     2341 ttggacgcga gaacggcgcc gggcttcatc acctcgtcca atcgcctgaa caccgcttcc
     2401 ttgaccgcga tgtcttcgaa caccgcctcg atcaccatgt cggcgtcctt cagatcgtcg
     2461 taggagaggg tcggctcgag gagcgccatg cgctcctgga gctgctgcgc ggtgagcttg
     2521 cctttcctcg cgctcgcctc gtagttcctg cggatggccg caagccccct gtcgagcgcc
     2581 tcctgcttca tctccagcaa ggtgacggga atgccggcgc tgaggaaatt catcgtgatg
     2641 cccacgccca tggtccccgc accgacgacc gccattttct cgaccttgcg cagcggcgtg
     2701 tcctccggca catcggggat cttcgccgcc gcgcgctccc cgaggaaagc atgccgcagc
     2761 gcgcgcgatt gcggcgtctg catgagctgc aggaacgatt cgcgctcgaa ccgtgccccc
     2821 tcttcatacg gcatggtgac tgcggccgcg acggcatcga cgcatttgag cggcgcgggg
     2881 aaatttttcg ccgccgcgcg gacggtattg cgtgcgaact ggaagaatcc gtccgcgttg
     2941 gcgagacttg ctttcaggtc gcgcacgcgc ttcagagggc gtttcccgga agcaactttg
     3001 tccgcgaagg cgatcgcccc cggcagcaga tccttctcga agaactcgtc gaacagcccg
     3061 ctgtccttga gctgctccga aggcacggaa ttgccggaga cgatcatgtt gagcgccgct
     3121 tccgcgccga tgagccgggg caggcgctgc gtgccccccg cgccgggaat cagccccagt
     3181 ttcacttcgg gcaaggcgat ctgggcgccc ttcacggcga cgcggtaatg acaacccagg
     3241 gccagttcca gcccgccgcc catgcaggcg ccggcgatgg ccgcgacgac gggtttcccg
     3301 gcattttcca gcatgcggat caccgtgttg agactgggtt ccgcggaagc cctgggcgag
     3361 ccgaactccc ggatatccgc cccgccggag aacgcgcgag cgctgccgat cagcaccacg
     3421 gccttcacgt tcgcatcggc ttcggcgcgg gccagccctt ccacgatgtc gtgccgcaga
     3481 tcgaaaccca gcccgttgac gggcgggttg tccatcgtga tcagggcgac cgtgccgcgc
     3541 agttcgtaga ttgtggcgcc catgcggtct gcctcgtttc caccgtcacc acgattatcg
     3601 caccaggggc aattttcgat tttgctcgag gttcctgtag gtacttacct gacatgaccg
     3661 tcgggtcata cccgaccccg gcgatcaggt ttcccccgat ggacactctc gccgtagttc
     3721 catagtctca gtgcgttcca atctttttgt cattccgtca gggcagtccc cgaaaagaag
     3781 aagatatttt ttagggaagg gagaaccggt ttggaacaag cgcaactgga aacactgcta
     3841 cccgacgttc agcacctcat cgagctgatc atcgacaacg tcaaccagct caaggacacc
     3901 cagggactga agg
//
LOCUS       DASPZD010000260         3474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1141767, whole genome shotgun sequence.
ACCESSION   DASPZD010000260 DASPZD010000000
VERSION     DASPZD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141767"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VEM33_05045"
     CDS             <1..619
                     /locus_tag="VEM33_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYS51222.1"
                     /translation="FKGASVTGNPVRQEIAAIAAPESRYAGRSGPLRVLVVGGSLGAK
                     ALNDIVPRALSHVPGGRRPIVTHQSGAQHLDSLRAAYSAAGVPAKTSAFIEDMAAAYA
                     ETDLVVCRAGATTVAELAAAGVASVLVPYPHAVDDHQTANARFLADAGAAVLVPQSEL
                     SPERLAGVLAGFDRLRLGEMSRRARSLARPGAAAAVATACAELAA"
     gene            616..2013
                     /gene="murC"
                     /locus_tag="VEM33_05050"
     CDS             616..2013
                     /gene="murC"
                     /locus_tag="VEM33_05050"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HYS51223.1"
                     /translation="MKHKVKRIHFVGIGGSGMSGIAEVLLNLGYKVSGSDLAANAATE
                     RLAGLGAKVVLGHNADNIASADAVVVSSAVTADNVEILAAKAKRVPVVPRALMLAELM
                     RLKQGIAVAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLVSAASNARLGTGEFIVVE
                     ADESDASFLQLQPVAAVVTNIDADHMETYQHDFSRLKQAFVGFLQNLPFYGSAIVCID
                     DANVREILPSVSKPIVTYGMREDAMVRGTEIIPGDRMRFRALREGAATLDITLNLAGR
                     HNVQNALAAIAVATELGVPDAAIRKALAEFRGVGRRFQRYGEVPLPPNGKSAGSFTLV
                     DDYGHHPVEMQATLDAARGAFPGRRIVLAFQPHRYTRTRDLFEDFVKALSGVDALLLA
                     EVYPAGETPIVAADGRSLARAIRIAGKTEPVFVERIADMPDAIRKIVRPGDVVLTMGA
                     GSIGGVPVALARTGR"
     gene            2016..2936
                     /gene="murB"
                     /locus_tag="VEM33_05055"
     CDS             2016..2936
                     /gene="murB"
                     /locus_tag="VEM33_05055"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYS51224.1"
                     /translation="MTEPIDRSGVALRGSLRLNEPMAKHVSWRAGGAAERAYVPADRA
                     DLAAFLATLPRGERVCFLGLGSNLLVRDGGFHGTVILMHSPHGVIRVEGGLIYAEASA
                     ACPKVARLTATRGFEGAEFLAGVPGTVGGALAMNAGCYGSETWDVVAKVLTISRAGKL
                     SERKPADFEVGYRRVTLRGSELGENEWFLAAWFRFHTGDEKRARARIKELLAKRIAEQ
                     PLELPNAGSVFRNPPGKYAARLIEACGLKGHEIGGARVSEKHANFIVNPKGRARASDI
                     EALIEHVRASVKERFGIELVPEVRIIGEAA"
     gene            2963..>3474
                     /locus_tag="VEM33_05060"
     CDS             2963..>3474
                     /locus_tag="VEM33_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HYS51225.1"
                     /translation="MGGRSAERAVSLKSGSMVLGALKKKGVDAHAFDTKERGLDALVK
                     ERFDRVFIALHGRYGEDGTVQGALELLGIPYTGSGVLGSALAMDKWRTKLAWQGSGIP
                     TPHYELLTRETDLHGVATRLGLPLMIKPANEGSSIGMTKVRSARDLPEAYALAANYDR
                     VVIAETFVDGV"
BASE COUNT          565 a         1135 c         1207 g          567 t
ORIGIN      
        1 gttcaaaggc gcaagcgtta ccggcaaccc ggtgcggcag gagatcgcgg cgattgcagc
       61 cccggagagt cgttacgccg ggcgctcggg cccgcttcgc gtgctcgtcg tcggcgggag
      121 cctcggcgcc aaggcgctga acgacatcgt tcccagggcg ctgtcgcacg ttcccggcgg
      181 gcggcggccg atcgtgaccc atcagtcggg ggctcagcac ctcgattcgt tgcgggccgc
      241 ctactccgcg gccggcgtac ccgcgaagac ctccgccttc atcgaggaca tggctgcggc
      301 gtatgccgag acggacctcg tcgtatgtcg cgctggcgcg accacggtcg cggagctagc
      361 cgctgcgggc gtggcgagcg tcctggttcc ctatccgcac gcggtggacg accaccagac
      421 cgccaacgct cgctttctcg cggacgcggg cgccgcggtc ctggtgccgc agagcgagct
      481 cagtcccgag cggctcgcgg gggtgctcgc cgggttcgat cgcttgcgtt tgggcgaaat
      541 gtccaggcgc gcgcgctcgc tcgcccggcc cggggctgcg gcggcggtcg ccacggcctg
      601 cgcggagctt gccgcatgaa gcacaaggtc aagcggattc attttgtcgg catcggcggc
      661 agcggaatga gcggcatcgc ggaagtgctg ctcaatctgg gctacaaagt gagcggctcg
      721 gacctcgctg cgaatgcggc gaccgaacgg ctcgcgggtc tcggggcgaa ggtcgtgctc
      781 ggccacaacg ccgacaacat cgcgtcggcg gacgccgtgg tcgtgtcctc cgcggtgacc
      841 gctgacaacg tcgagatcct cgccgcgaag gcgaagcgcg tcccggtcgt gccgcgcgcc
      901 ctgatgctcg ccgagctcat gcgcttgaag cagggcatcg ccgtcgcggg gacacacggc
      961 aagaccacga cgaccagcct cgtcgcgagc gtactcgccg aaggcgggct cgatcccacg
     1021 ttcgtgatcg gcgggcggct ggtgagtgcc gcctccaacg cgcggctcgg gaccggcgag
     1081 ttcatcgtcg tcgaggccga cgagtccgat gcctcctttc tccagttgca acccgtcgcc
     1141 gcggtggtga ccaatatcga cgccgatcac atggagacct accagcacga tttttcgcgg
     1201 ctgaagcagg cgttcgtcgg tttcctgcag aacctcccct tctacggcag cgccatcgtc
     1261 tgcatcgacg acgccaacgt gcgcgagatc ctgccctcag tctcgaagcc gatcgtgacc
     1321 tacgggatgc gcgaggacgc gatggttcgg gggaccgaga tcattccggg agaccgcatg
     1381 cgcttccggg cgttgcggga aggcgccgcg accctggaca tcacgctcaa tcttgcggga
     1441 cggcacaacg tgcagaacgc gctcgccgcg atcgccgtgg ccaccgagct cggcgttccc
     1501 gatgcggcga tccgaaaggc gctcgccgag ttccgcggcg tcggcaggcg tttccagcgc
     1561 tacggtgaag ttccgcttcc tccaaacggt aaatcggcgg ggagcttcac tttggtcgac
     1621 gactatgggc atcacccggt cgagatgcag gcgacgctcg atgcggcgcg cggcgctttt
     1681 ccgggtcggc gcatcgtgct cgcctttcag ccccaccgtt atacgcgcac gcgcgatctg
     1741 ttcgaggact tcgtgaaggc gctttccggc gtggacgcgc tgctgctcgc ggaagtctat
     1801 ccggcgggcg agacgccgat cgtagccgcc gacgggcgct cgctcgcccg cgcgatccga
     1861 attgcgggca agaccgaacc tgtattcgtc gagaggatcg ccgacatgcc cgatgccatc
     1921 cgcaagatcg tgcgcccggg tgacgtcgtg ctgaccatgg gtgcgggttc gatcggcggc
     1981 gtgcctgtag cgctcgccag gaccggaaga tgaagatgac tgagccgatc gaccgatcgg
     2041 gagtcgcgct gcgcggttcc ttgcggctga acgagccgat ggcgaagcac gtcagctggc
     2101 gcgcgggtgg tgccgccgaa cgggcgtacg ttccggccga ccgcgccgac ctcgccgcat
     2161 tcctcgcgac tcttccccgg ggcgaacgag tctgcttcct gggactcgga agcaatctgc
     2221 tggtacgtga cggcggattt cacgggacgg tcatcctgat gcacagcccg cacggcgtga
     2281 ttcgcgtcga gggtggcttg atttacgcgg aagcgagcgc cgcctgtccc aaggtggcgc
     2341 ggctcaccgc gacacgcggc ttcgagggcg cggagttcct cgccggagtt cccggaacgg
     2401 tgggcggggc gctcgcgatg aacgccggat gctacgggtc ggaaacctgg gacgtcgtcg
     2461 cgaaggtgct gacgatatcg cgcgccggga aattgagcga gcgcaagccc gccgatttcg
     2521 aagtcggata ccggcgcgtc acacttcgcg gatcggaact gggcgagaac gaatggttcc
     2581 tcgcagcttg gttccgtttc catacgggtg atgagaagcg cgcccgagcc cgcatcaagg
     2641 aactgctcgc gaagcgtatc gcggagcagc ctctcgaact tccgaatgcc ggctccgtgt
     2701 tccgcaaccc gcctggcaag tacgccgcgc gcctgatcga ggcctgcggg ctcaagggcc
     2761 atgagatcgg tggcgctcga gtctcggaga aacacgcgaa tttcatcgtc aacccgaagg
     2821 ggagagcgcg tgcctcggac atcgaggccc tgatcgagca cgtgcgcgcc tccgtgaagg
     2881 aacgcttcgg catcgagctg gttcccgaag tgcgaatcat cggtgaggcg gcgtgagggg
     2941 cttcggaaaa atcgccgtgc tcatgggtgg gcgctccgcc gagcgcgccg tctcgcttaa
     3001 gagcggttcg atggtgctgg gcgccctgaa gaagaagggg gtggacgccc acgccttcga
     3061 cacaaaagag cggggactcg acgcgctagt caaagagcgg ttcgaccgcg tcttcatcgc
     3121 gctgcacggc cgctacggcg aggacggcac ggtccagggc gcgctcgagc tgctcggaat
     3181 cccgtacacc ggctcgggcg tgctggggag cgcgctcgcg atggacaagt ggcgcaccaa
     3241 gctcgcctgg caggggagcg ggattccgac gccgcactac gagcttctca cgcgcgaaac
     3301 cgatctccat ggcgtcgcga cgcggctggg gctgcccctg atgatcaagc ccgccaacga
     3361 aggctcctcg atcggaatga ccaaagtaag gagcgcccgc gatctcccgg aagcctacgc
     3421 gcttgccgcc aactacgacc gcgtcgtgat cgccgagacg ttcgtcgacg gcgt
//
LOCUS       DASPZD010000261         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_736682, whole genome shotgun sequence.
ACCESSION   DASPZD010000261 DASPZD010000000
VERSION     DASPZD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736682"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..875)
                     /locus_tag="VEM33_05065"
     CDS             complement(246..875)
                     /locus_tag="VEM33_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYS51226.1"
                     /translation="MRDPYDTYMNTLVPMVVEQTNRGERAYDIFSRLLKERIIFISGP
                     VEDVTSTLVVAQLLFLEADNPKKEIAMYINSPGGVVTSGLAIYDTMQFVRPAVSTLCI
                     GQAASMGSLLLAAGHKDMRFALPNSRIMLHQPSGGFQGQATDIMLHAQEILNLKKRLN
                     EIYVKHTGQPLKKIEEALERDYFLTGEMAVEWGVVDKVIDKRPEDVSKA"
     gene            complement(868..1050)
                     /locus_tag="VEM33_05070"
     CDS             complement(868..1050)
                     /locus_tag="VEM33_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HYS51227.1"
                     /translation="MQSTLRCSFCGKDADQVGRLVAGPSVYICDECIKTCVAVVEQHG
                     GFPPSRAAEQDGLNHA"
     gene            complement(1174..>2545)
                     /gene="tig"
                     /locus_tag="VEM33_05075"
     CDS             complement(1174..>2545)
                     /gene="tig"
                     /locus_tag="VEM33_05075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYS51228.1"
                     /translation="MEVTETLSDGLKREFQVQVPAADLEARVTERLGELKDRVNLRGF
                     RPGKVPVTHLRRIYGKAVMAETIEAVIRELNAQIVSERGLKLAMEPKVTIPNEASAVE
                     KVIGGQSDLAYTLALEILPKIELADFKGIKLERQVAEVTEAQVNDALGKIAEQNRPFA
                     VKAEGAKVEKGDRILIDFAGRIDGAPFEGGTGGDVGVNVGAGTFLPGFEDQLVGMTVG
                     ETRVVKVTFPDNYLNTQLAGKNAEFDVTAKSLEAPGQVTLDDAFAKSLGLESLDKLKE
                     AVKGRLQQEHAALSRQKLKRKLLDKLDEMHKFALPPTLAEDEFKNVWTTVENDLKAQG
                     RTFADEGTTEEKAREEYRGIAERRVRLGLVLAEIGEKNKITVTEEEITRAIVERARQV
                     PGREQEVWEYYRKTPAAVAAIRAPIFEEKVVDFLIELATVTERQVSREDLLKDEEDEN
                     SAAGRS"
BASE COUNT          435 a          842 c          724 g          544 t
ORIGIN      
        1 ccccgcgcgc gaaccaaccg ccttgccgcc caccataaca ggcgctcttt taagagtccg
       61 tatcaccaat gtcacacgcc tccagggttt ttgatcctcc aacctcgcca aatcacccta
      121 gcgaaccacg gtactgccgg cagaatcaag attttattaa tcaaccaaaa gtcaattcca
      181 ggctttccac cgtcgcgcag cccggcgcgg aaccaccggc ggtggaatac cgtttgggcc
      241 gtcgatcacg ccttggagac atcctcaggc cgcttgtcga tcaccttatc gaccaccccc
      301 cattcgaccg ccatctcacc ggtcaggaag tagtcgcgct cgagcgcctc ctcgatcttc
      361 ttcagcggct gcccggtgtg cttcacgtag atttcgttca ggcgcttctt caggttcagg
      421 atctcctggg catgcagcat gatgtccgtc gcctggccct gaaatccgcc ggacggctgg
      481 tgcagcatga tcctggaatt gggaagcgca aagcgcatgt ccttgtgtcc ggcggcgagc
      541 agcagcgagc ccatggacgc cgcctggccg atgcacaagg tcgagaccgc cggccgcacg
      601 aactgcatgg tgtcgtagat cgcaagcccg gaggtcacca cgccgcccgg cgagttgatg
      661 tacatcgcga tctctttctt cggattgtcc gcctcaagga acaagagctg agcgaccacg
      721 agcgtcgagg tgacgtcctc gactggcccg ctgatgaaga tgatccgctc cttgagaagc
      781 cgcgagaaga tatcgtaggc gcgctcgccg cgattggttt gctcgaccac catcggcacg
      841 agcgtattca tgtaggtgtc gtatggatca cgcatggttc aacccatcct gctcggcggc
      901 tcgcgacggc gggaagcccc cgtgctgctc caccaccgcc acgcatgttt tgatgcactc
      961 gtcacagata tatacggacg ggcccgcgac aagccggcca acctggtcgg cgtctttgcc
     1021 gcagaacgaa cagcgcagtg ttgattgcat ccgcagcaat cgggcgatga cggcaagctt
     1081 gcgtgccatg ctcctatatc cacgattcgc gccgacacaa caccaaggcg ggtgtttgac
     1141 cctgcttcgg cggtagccca acgcggcgct tggtcatgat cgacctgcgg cgctgttttc
     1201 gtcctcctcg tccttgagca ggtcctcgcg cgagacttgc ctctcggtga cagtggctag
     1261 ctcgatgagg aagtccacca ccttctcttc gaagatcggg gcccgtatgg ctgcgaccgc
     1321 ggcaggagtc ttgcggtagt attcccagac ctcctgctcg cgcccgggca cttggcgggc
     1381 gcgttcgaca atggcgcgcg tgatctcctc ctcggtcacg gtgatcttgt tcttctcgcc
     1441 gatttccgcg agcacgaggc caagccgcac ccgccgttcc gcaatgccgc gatattcctc
     1501 gcgcgccttc tcctcggtcg tgccctcgtc ggcaaaggtg cggccctgcg ccttgaggtc
     1561 gttctcgacc gtggtccaga cgttcttgaa ctcgtcctcg gcgagcgtcg gaggcagcgc
     1621 gaatttatgc atctcgtcca gcttatcaag gagcttgcgc ttgagcttct gccggctgag
     1681 cgccgcgtgc tcttgctgca gccgcccctt gaccgcctcc ttgagcttgt cgagagattc
     1741 caaccccagc gacttcgcga atgcatcgtc gagggtaacc tggcccggcg cttcgagcga
     1801 cttcgctgtg acgtcgaact cggcgttttt ccccgcaagt tgcgtgttca ggtaattgtc
     1861 gggaaatgtg accttcacca cgcgcgtttc gccaaccgtc atgccgacga gttgatcctc
     1921 gaagcccggg aggaacgttc cggccccgac attgacgccc acatcgccgc ccgtgccacc
     1981 ttcgaaaggc gcgccgtcga tcctgccggc aaagtcgatg aggatgcggt ctcccttttc
     2041 gactttcgcc ccttcggctt tgacggcaaa cggccgattc tgttcagcga tcttccccag
     2101 cgcatcattg acttgcgcct cggtgacctc cgccacctgc cgctcgagct tgataccttt
     2161 gaaatccgcg agctcgatct tcggcagtat ctccagcgca agcgtgtagg cgagatcgga
     2221 ttgtccgccg atcacctttt cgaccgcgct ggcttcgttc gggattgtga ccttgggctc
     2281 catggcgagc ttgagaccgc gctcgctgac gatctgggcg ttgagctcgc ggatcaccgc
     2341 ctcgatggtt tcggccatca ccgcctttcc gtaaatcctc ctcaggtgag tgacggggac
     2401 cttgccggga cggaagccgc gcaggttgac ccggtccttg agctcgccca gccgctcggt
     2461 gactcgcgcc tcgagatcgg ctgcgggcac ctgaacttgg aattcgcgct taaggccgtc
     2521 ggaaagggtc tcggtgactt ccatg
//
LOCUS       DASPZD010000262         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1193551, whole genome shotgun sequence.
ACCESSION   DASPZD010000262 DASPZD010000000
VERSION     DASPZD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193551"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VEM33_05080"
     CDS             <1..545
                     /locus_tag="VEM33_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HYS51229.1"
                     /translation="AAARSLAAEFGAACAGRGLRLVYGGSGRGLMGIAARAAAAAGGE
                     VLGIMPRHLVRPERAADNIGTLQIVDSLAERKQKMAEASDLFVAIPGGIGTLNELLEM
                     LTMNDLGLQDKPVILCASDGFWQPFIAMIERFRAYGVLRPSVERRLHAAASVGEAMRL
                     IEDHLSSAARGTQAAASRGV"
     gene            558..633
                     /locus_tag="VEM33_05085"
     tRNA            558..633
                     /locus_tag="VEM33_05085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:591..593,aa:Arg,seq:ccg)
     gene            674..1984
                     /gene="ugpB"
                     /locus_tag="VEM33_05090"
     CDS             674..1984
                     /gene="ugpB"
                     /locus_tag="VEM33_05090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HYS51230.1"
                     /translation="MRTKIRVGVLAGAVLLSGTVRAQTEIYWWHSMQGALNDKVNELA
                     NGFNASQKEYKVVAIFKGQYPESMTAAIAAFRAGNAPHILQVFEVGTATMMGAKGAIK
                     PVYELMKEAGEPFDAKNYLPAVAGYYTDSKGNMLSLPFNSSTPVFYVNKDAFKKAGLD
                     GTAPKTWKEFAAVAGKLKASGQQCVYSTGWPAWVHVENFSAWHNLPIGTRENGIAGTD
                     TEFRINSPQHVRHVEMLADFAKKGLFTYSGRKNEAEARFFSGECAMLTSSSGAQANIR
                     RNAKFDFSVNFLPYHDDIKGAPQNSIIGGASLWVMTGRKPAEYKGVTKFFSYLSSTGV
                     QVDWHKSTGYVPITNAAYEMTKKDGYYEKVPGADIAVRQLANKAPTPNSKGLRFGNYV
                     QGREVIEEEMEGVFAGKKDAKTAMEDAARRGNDILRKFQAANKN"
     gene            2022..2903
                     /gene="ugpA"
                     /locus_tag="VEM33_05095"
     CDS             2022..2903
                     /gene="ugpA"
                     /locus_tag="VEM33_05095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HYS51231.1"
                     /translation="MEKRVVFRSAWLPYALLTPQIAVTIVFFFWPAAQAFWFSFQLQD
                     AFGISTEFAGLRNFADLLRDERYLASFKSTAVFSLLVAASGIAISLALATMADRVLRG
                     ALAYKTLLIWPYAVAPAVAGVLWGFLFAPSIGIVTYVIRKAGYNWDWVLQGDQAMLLV
                     VMAATWKQISYNFLFFLAGLQSIPRSLVEAAAIDGAGPARRFWTIVFPLLSPTTFFLL
                     VVNIVYAFFDTFGVIDSTTQGGPAGATQILVYKVYYDGVKAGDLGGSSAQSVILMLIV
                     IALTVAQFRFIEKKVQY"
     gene            2915..3763
                     /gene="ugpE"
                     /locus_tag="VEM33_05100"
     CDS             2915..3763
                     /gene="ugpE"
                     /locus_tag="VEM33_05100"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HYS51232.1"
                     /translation="MVENRPWLTVMSHAVLIAGIAVIAFPLYVTFVASTLTLDQILQV
                     PMPLAPGDRLWENYSKALTAGASGAAAAPVGRMMLNSLVMAVAIALGKIAISLIASFA
                     IVYFRFPLRNFFFWMIFVTLMLPVEVRIIPTYKVVSDLGMLNSYVGLTLPLIASATAT
                     FLFRQFFLTIPEELAEASRIDGAGPMRFFFDVVLPLSKTSIAALFVIQFIYGWNQYLW
                     PLLVTTDESMYTVVIGIKRMILGGDALTEWNVVMATAMLALLPPAAVVVLMQKWFVKG
                     LVETEK"
BASE COUNT          677 a         1197 c         1205 g          712 t
ORIGIN      
        1 cggccgccgc gcgctccctc gccgcggaat tcggcgctgc ctgcgcgggg cgcggactgc
       61 gcctcgtata cggcggcagc ggccgggggc ttatggggat cgcagcgcgc gccgccgcgg
      121 ccgcgggtgg cgaagtgctc gggatcatgc cgcgccatct ggtgcggccc gagcgcgccg
      181 cggacaatat cggcacgctc cagatcgtgg actcgctcgc tgagcgcaag cagaaaatgg
      241 ccgaagcttc cgacctcttc gtcgcgattc ccggcggaat cggaacgctc aacgagctgc
      301 tggagatgct tacgatgaac gacctgggac tgcaggacaa gccggtgatc ctgtgcgcaa
      361 gcgacggctt ctggcagccg ttcatcgcga tgatcgagcg cttccgcgcc tacggcgtgc
      421 tgcggccgag cgtggagcgc aggctgcacg cggccgcttc agtcggggaa gccatgcgcc
      481 tcatcgaaga ccatctgtcg tctgcggccc gcgggacgca ggccgccgcg tcgcgcggtg
      541 tataatttcc gctccccgcg cccgtagctc aggggataga gtgatggcct ccgaagccat
      601 aggtcgcgcg ttcgattcgc gccgggcgcg ccagattcca ccgcgctcca ataccgtaaa
      661 gtagggaggc agcatgcgta ccaagattcg cgttggcgtc ctggctggcg ccgtcctgct
      721 gtccggaacg gtacgagccc agaccgaaat ctactggtgg cattcgatgc agggggcgct
      781 caacgacaag gtcaacgagc tcgccaacgg cttcaacgcc agtcagaagg aatacaaggt
      841 cgttgcgatc ttcaagggac aataccccga gtcgatgacc gcggcgatcg ccgcgtttcg
      901 tgctggcaac gcgccgcaca ttctgcaggt gttcgaggtc gggaccgcga ccatgatggg
      961 tgcgaagggc gcgatcaagc ctgtctacga gctgatgaag gaggcgggcg agccgttcga
     1021 cgcgaagaac tatctccccg cggtggcggg ttactacacc gacagcaagg gaaacatgct
     1081 gtcgctgccg ttcaacagct cgactccggt gttctacgtc aacaaggatg cgttcaaaaa
     1141 agccggcctc gatggcaccg caccgaagac ctggaaggaa ttcgcagccg tcgccggcaa
     1201 gctgaaggcc tccggacagc agtgcgttta ttccaccggc tggcccgcgt gggtgcatgt
     1261 cgagaacttc agcgcctggc acaacctgcc gatcggcacc cgggaaaacg ggatcgccgg
     1321 cactgacacg gaatttagga tcaattctcc gcagcacgtg aggcacgtcg agatgctggc
     1381 cgacttcgcc aagaagggcc tgttcaccta ttcggggcgc aagaacgaag ccgaggcgcg
     1441 ttttttcagc ggtgagtgcg ccatgcttac ctcgagttcg ggcgcgcagg cgaacattcg
     1501 ccgcaacgcg aagttcgact tctcggtcaa cttcctgccc tatcacgacg acatcaaggg
     1561 cgcgccgcag aattcgatca tcggcggcgc gagcctctgg gtcatgaccg gcaggaagcc
     1621 tgccgagtac aagggcgtaa cgaagttttt ctcgtatctg tcctccacag gcgtgcaggt
     1681 cgactggcac aaatcgacgg gttatgtgcc gatcaccaac gccgcctacg agatgaccaa
     1741 gaaggacggt tattacgaga aagtccccgg cgccgacatt gccgtgcgcc agctcgccaa
     1801 caaggcgccc acgcccaatt cgaagggcct gcgtttcggc aactacgtgc agggccgcga
     1861 ggtgatcgaa gaggagatgg aaggcgtatt cgccggcaag aaggatgcga agacggcgat
     1921 ggaagacgcg gcgcgccgcg gcaacgatat cctgcggaaa ttccaggcgg cgaacaaaaa
     1981 ctgacgaggg gaaacggggc ggggcccctt atcacccgag aatggaaaag cgcgtcgtat
     2041 tccgctcggc gtggttgcct tacgcgctgc tcacgccgca gatcgccgtc accatcgtat
     2101 tcttcttctg gccggcggcg caggccttct ggttctcctt ccagctgcag gatgccttcg
     2161 gcatcagcac cgagttcgcg gggctgcgca acttcgccga ccttctgagg gacgagcgtt
     2221 atctcgcgtc cttcaagagc acggccgtat tcagcctcct ggtcgccgcg agcggcatcg
     2281 ccatctcgct cgcgctcgcc accatggccg accgcgtgct gcgcggcgcc ctcgcctaca
     2341 agacgctcct catctggcct tacgccgtcg cgccggcggt ggccggcgtg ctctgggggt
     2401 tcctgtttgc gccttcgatc ggcatcgtca cctacgtgat caggaaagcg ggctacaact
     2461 gggattgggt gctgcagggc gaccaggcga tgctgctggt ggtgatggcg gcaacctgga
     2521 agcagatcag ctacaacttc ctgttctttc tcgccggact gcaatccatc ccgcgctcgc
     2581 tggtggaggc ggcggccatc gacggggcgg gccccgcacg ccgtttctgg accatcgtct
     2641 ttccgctgct gtcgccgacc acgttctttc tgctcgtcgt caacatcgtg tacgcgtttt
     2701 tcgacacgtt cggagtgatc gattccacga cccagggcgg gccggcgggc gcgacccaga
     2761 ttctcgtcta caaggtgtat tacgacggcg tcaaagcggg cgatctaggc ggctcgtcgg
     2821 cgcagtcggt catcctgatg ctgatcgtca tcgcgctcac ggttgcgcaa ttcaggttca
     2881 tcgagaaaaa agtgcagtac tgaatggtcg acgcatggta gagaacagac cctggctgac
     2941 ggtcatgtcg cacgccgtgc tgatcgcggg aatcgcggtc atcgcctttc cactgtatgt
     3001 caccttcgtc gcctcgacgc tcacgctgga tcagatcctg caggtgccga tgccgctcgc
     3061 gccgggggac cgcctttggg agaactattc gaaggcgctc accgctggag cgagcggcgc
     3121 ggcggccgcc cccgtgggcc ggatgatgct gaacagcctg gtgatggcgg tcgcgatcgc
     3181 gctcggcaag atcgccatct cgctcatcgc ctcgttcgcg atcgtctact tccgcttccc
     3241 gctccggaac ttcttcttct ggatgatctt cgtcaccctg atgctgcccg tcgaggtgcg
     3301 catcatcccc acctacaagg tggtctccga tctcggcatg ctgaattcct acgtcggcct
     3361 gacgctgccc ttgatcgcct cggcgacggc cactttcctg ttccggcagt tcttcctcac
     3421 gatcccggag gagctcgccg aggcctcgcg catcgacggc gccgggccga tgcgcttctt
     3481 tttcgacgtg gtgctgccgc tttcgaagac cagcatcgcg gcgctgttcg tgatccagtt
     3541 catctacggg tggaaccagt acctgtggcc tttgctcgtc acgacagacg agtcgatgta
     3601 caccgtggtg atcggcatca agcgcatgat cctcggcggc gatgcgctca ccgagtggaa
     3661 cgtcgtcatg gcgaccgcca tgctcgccct gctgccgccg gccgcggtcg tcgtgctgat
     3721 gcagaagtgg ttcgtcaagg gcctggtcga gacggagaaa taatggcgcg ggtatcgcta
     3781 cgagacgtga a
//
LOCUS       DASPZD010000263         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_22396, whole genome shotgun sequence.
ACCESSION   DASPZD010000263 DASPZD010000000
VERSION     DASPZD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22396"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..795)
                     /locus_tag="VEM33_05105"
     CDS             complement(10..795)
                     /locus_tag="VEM33_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYS51233.1"
                     /translation="MKPARARIALTVLSILVAFVAFAVWFMTAMPGTRHRGPLQPLAV
                     GDQQILANLKAHVAAVASEEHNAGHPEALERSARYIEATLSGLGYAVSRQEFETGGVK
                     VRNIEVKRAGTDAGKARLVVIGAHYDSAQGAVGADDNGSGVAALLELARLLESVKPAE
                     GLEMRLVFFVNEELPWFATEKMGSLVHAGGLAREGMEVVAMLSLETIGWYSDAQGSQR
                     YPFPFNLLYPSTGDFIGFVANPRSRSLLHRVIGSFRRHAAFPS"
     gene            complement(798..1232)
                     /locus_tag="VEM33_05110"
     CDS             complement(798..1232)
                     /locus_tag="VEM33_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYS51234.1"
                     /translation="MQVSEILKVKGNALFTIAPDGKLTEAVSIMAQHNMGSLVVIDKG
                     RMVGMLTFEEVLSALDKGRGALGDARVRDSMERDPVTASPAMEVNDLRRTMIDSGARY
                     LPVMDRDKLLGVISFRDVAKAVLEEQDFENRMLKGYIKNWPE"
     gene            1415..2248
                     /locus_tag="VEM33_05115"
     CDS             1415..2248
                     /locus_tag="VEM33_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51235.1"
                     /translation="MGRMPPAALVAARNLAHHAAQWPAKAAHANLSAAPDDSHSAFTW
                     DASHAALVSRILPAKGGGVRVGIRMARLELIVTSGDSVLDALQFDGKTDAAAGDWLDS
                     KLHALGLKPAGGVRLPYAPPAHPTGGRPHELGMLGRELGELSRWFGGPAEMLEEFAGK
                     LAGLRPGPGPLLCWPHHFDIATLVRLDSGAGEAARSVGVGVSPGDEFYAQPYVYVNPW
                     PRLDGGNLPALPPPGHWHTEGFFGAVATGEEILAMKDRGKALLAFITGAFEIGRAGLG
                     A"
     gene            2251..>2607
                     /locus_tag="VEM33_05120"
     CDS             2251..>2607
                     /locus_tag="VEM33_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51236.1"
                     /translation="MMRKRGVALLFALACAGHGAAVLAQAYPSRPPRLIVGFTPGGGV
                     DINARVLASKLSELLSQQVIVENRPGAGTNIANEYVAKSAPDGYTLLFNSPAVVINMS
                     LYRNPPYDALRDFAGVS"
BASE COUNT          415 a          885 c          868 g          439 t
ORIGIN      
        1 caccgcgccc tacgagggaa acgccgcgtg gcgcctgaac gagccgatga cccggtgcag
       61 gaggcttcgc gagcgcggat tggcgacgaa gccgatgaaa tcgccggtgg acgggtagag
      121 cagattgaag ggaaacgggt agcgctggct gccctgcgca tcgctgtacc agccgatggt
      181 ctcgagggac agcatcgcga cgacttccat cccctcgcgg gcaaggccgc ccgcgtgcac
      241 gaggctgccc attttttccg tcgcgaacca gggcaattcc tcgttgacga agaacaccag
      301 gcgcatctcc aggccctcgg cgggcttgac cgattcgagc aggcgcgcaa gctcgagcag
      361 ggccgccacg cccgagccgt tgtcatcggc cccgacggcg ccctgcgccg aatcgtaatg
      421 cgccccgatc acgacgagcc gtgccttccc cgcatccgtg ccggcgcgct tcacctcgat
      481 gtttctgacc ttcacgcccc ccgtttcgaa ttcctggcgg gagacggcgt agcccaggcc
      541 cgagagcgtc gcctcgatgt agcgcgcgga gcgctcgagc gcttcgggat gcccggcgtt
      601 gtgctcttcg ctcgccacgg cggcgacgtg cgccttgagg ttcgcgagga tctgctggtc
      661 tcctacggcg agcggctgca ggggaccccg gtggcgcgta ccgggcatcg ccgtcatgaa
      721 ccagacggcg aacgcgacga atgcaacgag gatcgagagg acggtgagcg cgatacgggc
      781 tctcgcgggt ttcaaggcta ctcgggccag ttcttgatgt agcccttcag catgcggttc
      841 tcgaagtcct gctcctcgag caccgccttg gcaacgtcgc ggaaggaaat caccccgagc
      901 agcttgtcgc ggtccatcac cgggaggtag cgcgcgccgg agtcgatcat ggtgcgccgc
      961 aggtcgttga cttccatggc ggggctcgcg gtcaccggat cgcgctccat gctgtcgcgg
     1021 accctcgcgt caccgagcgc gccccggccc ttgtccagag ccgacagaac ttcctcgaag
     1081 gtgagcatcc cgaccatgcg gcccttgtcg attaccacga gcgagcccat gttgtgctgc
     1141 gccatgatcg aaacggcctc ggtcagcttg ccatcgggcg cgatggtgaa gagcgcgttg
     1201 cctttgacct tgaggatttc gctcacctgc atgggtcccc ctctcgctgc gcatttccca
     1261 cgcgataata ctcccggaga cccgcgcgct ccaacaggaa cgaatcgacg aacgatgcca
     1321 tttcgaatcg tggctcccgg ctcaatctgg taaggttgtt ctcgctcggc gcgacggagg
     1381 tccgaaactt cccgtgcgat tcgactggtc gagaatgggc agaatgccgc ccgcggcgct
     1441 cgtcgcggcg cgcaatctcg cgcatcacgc ggctcaatgg cccgccaagg ccgcgcacgc
     1501 gaacctgagc gcggcccccg acgacagcca ctccgccttc acttgggacg cgtcgcacgc
     1561 ggcactggtt tcccggattc tgccggcgaa aggcggcggg gtccgggtcg gcattcggat
     1621 ggcgcggctc gagctgatcg tcacgagcgg cgacagcgtg ctcgacgcgc tccagttcga
     1681 cgggaagacc gacgcggccg ccggcgactg gctcgactcc aagctgcacg ctctcggcct
     1741 gaagccggca ggcggcgtga ggctcccgta cgcgccgccc gctcacccga ccggcggccg
     1801 gccccacgag ctcggcatgc tgggccggga actgggagaa ctttcgcgct ggttcggcgg
     1861 cccggccgaa atgctggaag aattcgcggg gaagctcgcg ggcctgcggc ccggtcccgg
     1921 accgctgttg tgctggccgc accatttcga catcgcgacg ctggtgcggc tcgactcggg
     1981 cgcaggcgag gcggcccgct cggtgggcgt cggggtttct ccgggggacg agttctacgc
     2041 ccagccgtac gtgtacgtga atccctggcc gcgccttgac gggggaaacc tgcccgcgct
     2101 gccgccgccc ggtcattggc acaccgaagg cttcttcggc gcggtggcga cgggcgagga
     2161 aatcctggcc atgaaggatc gcgggaaggc ccttctcgcg ttcatcacgg gagcgttcga
     2221 gatcggccgc gccggtctgg gggcctagct atgatgcgga aacgtggtgt cgcattgctg
     2281 ttcgcactcg cgtgcgcggg gcacggcgca gccgttctcg ctcaagccta tccgagccgg
     2341 ccgccgcggc ttatcgtcgg gttcactccg ggaggcggag tcgatatcaa cgctcgcgtg
     2401 ctcgcatcga aactttccga gctcctttcc caacaggtga tcgtcgagaa tcgtcccggc
     2461 gcgggcacca acatcgcgaa tgagtacgtg gccaaatcgg cgcccgacgg gtacacgctc
     2521 ctcttcaaca gcccggcggt cgtgatcaac atgtcgctgt accggaaccc gccctacgac
     2581 gcgctgcgcg acttcgccgg cgtatcg
//
LOCUS       DASPZD010000264         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_588081, whole genome shotgun sequence.
ACCESSION   DASPZD010000264 DASPZD010000000
VERSION     DASPZD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588081"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VEM33_05125"
     CDS             <1..574
                     /locus_tag="VEM33_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYS51237.1"
                     /translation="LRSSLRQDPDVILIGEMRDVETAQIGLRAAITGHMVLSTLHTRD
                     AASAPLRLVDMGVPRYMVAYSLQAVVAQRLLRVNCESCSKAHQPEPQEAQWLRTFGRD
                     PAAGRIMAGSGCQHCNGSGYSGRIGIYEMLEMDNELAALANSDDPGRFIAAAEQRMAG
                     QLLRDSAAQLALTGKTTVSEAMRVTAQLED"
     gene            583..1821
                     /locus_tag="VEM33_05130"
     CDS             583..1821
                     /locus_tag="VEM33_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYS51238.1"
                     /translation="MPFFSYTGRNARGELVRGVLENSDSGAVADQLLTSGVSPIDISP
                     ASGPKTISGDGWLRRLGSERVTLEDMLLFSRQMYTLLKSGVPILKSLAALQESATRVA
                     FANMLQDVRASLDSGRDLSTAMRRHPKVFGPFYISMVRVGELTGALEEVFLRLFNHLE
                     FEREIKNQVSQALRYPIFVIAVMGAALVIVNIFVIPAFARIFASFKAELPLMTRALLG
                     FSEFMVDYWPALLGVVAGAYVMFRVWVGTPAGLYSWDRMKLRIPIAGKIVLKATLARF
                     ARSLSIALKSGVPIVQALSVVEQVVDNAYVGQRIGQIRDGVERGESLLRTAVTTGVFT
                     PVVLQMISVGEESGELDDLLREVAEMYQREVEYELKTLSSQIEPILIVLLGVLVLILA
                     LGVFLPMWNLGQAALAGGNR"
     gene            1802..2368
                     /locus_tag="VEM33_05135"
     CDS             1802..2368
                     /locus_tag="VEM33_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYS51239.1"
                     /translation="MQAATVSVLRPGFGPRERRRAVPGFTLIELIIVVCIVALCAALL
                     LDRLRFYQEAAEKAAMEYTVATMKSALQLRVAAMLVRGEEGNIGKLARSNPMDWLMEP
                     PVGYRGEFRAAQPAVPQGSWYFDATRNEIVYVPNLDAHLERLADGSKRLRFRVRLDFE
                     PKDPDSERRRFAGMRIEPVTPYTWFYRP"
     gene            2411..2869
                     /locus_tag="VEM33_05140"
     CDS             2411..2869
                     /locus_tag="VEM33_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYS51240.1"
                     /translation="MEETNVTKETRGFTLIELIVVILILGILAAIAAPKFVDLTGQAR
                     KAALNGVRAAVSSASTLANGLQVAQGLTPDQAVSIEGTNVLMKNRYPSQATGGIDAAV
                     RFDNSTFETAAPTVTFRVKTAGTPASCAFQYTDATATAPPTIAPSTTTGC"
     gene            3018..3461
                     /locus_tag="VEM33_05145"
     CDS             3018..3461
                     /locus_tag="VEM33_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYS51241.1"
                     /translation="MTKRTRGFTLIELIVVILILGILAAIAAPKFVDLTGQARTAALN
                     GLRAAVSSASTLANALQVAQGLTPDQAVTIEGGPVLMKNRYPSQATGGIDAAVRFDSS
                     TFETAAPTVTFRVKTAGTPASCAFLYSDATATAPPTIAPSTTTGC"
BASE COUNT          669 a         1121 c         1198 g          692 t
ORIGIN      
        1 gctgcgctcg tccctgcgtc aggacccgga cgtgatcctg atcggggaga tgcgcgatgt
       61 cgagacggcg caaatcggtc tgcgggccgc catcacgggc cacatggtgc tgtcgacgct
      121 gcacacccgc gacgcggcga gcgctccgtt gcgcctcgtc gacatgggtg tgccgcgtta
      181 catggtcgcc tattcgctgc aggccgtcgt ggcgcagcgc ctccttcgcg tcaactgcga
      241 atcctgcagc aaggcgcacc aacccgagcc gcaggaagcg caatggctgc gcaccttcgg
      301 gcgcgacccg gccgccgggc ggatcatggc gggtagcggc tgccagcact gcaacggcag
      361 cggctattcg gggcgaatcg gcatctacga aatgctcgaa atggacaacg agctcgcggc
      421 gctcgccaac tcggacgacc ccggccgctt catcgcggca gccgagcagc ggatggcggg
      481 ccagctgttg agggacagcg cggctcagct cgcgttgacc ggaaagacta cggtttccga
      541 agccatgcgc gtcacggcgc agctcgagga ctgaggctgc gcgtgccgtt cttctcctac
      601 accggtcgta acgcgagggg agaactggtt cgcggcgtgc tcgagaattc ggattcgggc
      661 gcggtcgccg atcagctcct cacgagcggt gtatccccga tagacatcag tcccgcctct
      721 ggtccgaaga cgatttcggg ggacggatgg ctgcgccggc tgggcagcga gagggtgacg
      781 ctcgaggaca tgctgctctt cagccggcag atgtatacgc ttctcaagtc gggggttccc
      841 atactcaaat ccctggcggc gctccaggaa tccgcgaccc gggtcgcgtt tgcgaacatg
      901 ctgcaggacg tgcgcgcgag cctggacagc ggcagggacc tctcgaccgc gatgcgcagg
      961 cacccgaagg tgttcgggcc gttctacatc agcatggtgc gcgtgggcga gctgaccggc
     1021 gctctcgagg aagtcttcct caggctgttc aaccacctcg agttcgaaag ggagatcaag
     1081 aaccaggttt cccaggcact gcgctatccg attttcgtca tcgccgtgat gggcgcggcc
     1141 cttgtcatcg tcaatatctt cgtcattccc gcttttgcaa ggatctttgc gagtttcaag
     1201 gccgaattgc cgctgatgac gcgggcgctg ctcggttttt cggagttcat ggtggattac
     1261 tggccggcgc tgctgggcgt cgtcgccggc gcctatgtga tgttccgcgt ctgggtcggc
     1321 acccccgcgg gcctttacag ctgggaccgg atgaagcttc gcataccgat cgccggcaag
     1381 atcgtgctca aggcgacgct ggcgcgcttc gcacgctcgc tgtcgatcgc cctgaagagc
     1441 ggggtgccca tcgtacaggc cctgtcggtg gtggagcagg tcgtggacaa tgcatatgtg
     1501 gggcagcgaa tcgggcagat ccgcgacgga gtcgaacgcg gggaaagcct gctgcggacc
     1561 gcggtgacca ccggggtgtt cacccccgtg gtgctgcaga tgatctcggt gggcgaggag
     1621 tcgggagagc tggacgatct gctgcgcgaa gtggccgaaa tgtatcagcg ggaagtcgaa
     1681 tacgagctga agacgctctc gtctcagatc gagcccatcc tgatcgtgct gctaggcgtg
     1741 ctggtgctga tactcgcgct cggggtattc ctcccaatgt ggaatctggg gcaagctgct
     1801 cttgcaggcg gcaaccgtta gcgtcctcag gccgggtttc gggccgcgcg agcgccggcg
     1861 cgccgtcccc ggatttaccc tgatcgagct gatcatcgtc gtgtgcatcg ttgcgctctg
     1921 tgccgcgctg ctgctcgatc gcctgcgttt ctaccaggaa gcagcggaaa aagcggccat
     1981 ggaatacacg gttgccacga tgaaaagcgc gttgcagctg cgggtcgcag ccatgctggt
     2041 ccgcggcgag gaaggaaata tcggcaaact cgcccgttca aaccccatgg actggttgat
     2101 ggaaccgccg gtgggctacc gcggggagtt ccgcgcggcg caacccgcgg tgccgcaagg
     2161 aagctggtat tttgatgcga cccggaacga gatcgtgtac gttcccaatc tggacgctca
     2221 cctagagcgc ctggcggacg ggagcaagcg gcttcgattt cgggtgcggc ttgattttga
     2281 gccgaaagac ccggattccg agcgcaggcg gttcgccgga atgcggatcg aacccgtgac
     2341 gccttatact tggttctaca ggccgtaggc acgagaattg cgcgtgaagc gatcataaga
     2401 tctcaaattc ttggaggaaa caaacgtgac gaaagaaact cgtggtttca cgctgatcga
     2461 gctgatcgtg gtgatcctga tcctcggtat tctggcggct atcgcggcgc ccaagtttgt
     2521 cgacctgacc ggacaggccc ggaaagctgc gctgaacggc gtgcgcgctg cggtcagttc
     2581 cgcctcgacg ctcgccaatg ggctgcaggt cgctcagggc ctcactcccg atcaggccgt
     2641 cagcatcgag gggacaaacg tgctgatgaa aaaccgctac ccgtcccaag ccacaggcgg
     2701 gatcgacgca gccgtgcgct tcgataactc gaccttcgag accgccgccc cgaccgtgac
     2761 gtttcgggtc aagacggcgg gcactccggc gagctgtgcg ttccaatata ccgacgcgac
     2821 cgcgactgcc ccgccgacta tcgcgccatc cacaaccacc ggctgctgat cggtggcggg
     2881 tctttcgatg attcctgatc gagaacactt tattcgcaac gggcggaggg aatttaacgg
     2941 gtggaaagag tttgtgtata atcagaatga gcgtgagaaa ggacctgtaa gctcttaaat
     3001 ttttgaggga aacgaaaatg acgaaacgaa cccgtggctt cacgctgatc gagctgatcg
     3061 tggtgatcct gatcctcggc attctggcgg ctatcgcggc gcccaagttt gtcgacctga
     3121 ccggacaggc gcgcacagcc gcattgaacg gcttgcgcgc tgcggtcagt tccgcctcga
     3181 cgctcgccaa tgcgctgcag gtcgcccagg ggctcactcc cgatcaggcc gtcaccatcg
     3241 aggggggacc cgtgctgatg aaaaaccgct atccgtccca agccacaggc gggatcgacg
     3301 cagccgtgcg cttcgatagc tcgaccttcg agaccgccgc cccgaccgtg acgtttcggg
     3361 tcaagacggc gggcactccg gcgagctgtg cgttcctgta tagcgacgcg accgcgactg
     3421 ccccgccgac tatcgcgcca tccacaacca ccggctgctg acctgcgggc gcaactaacc
     3481 gattcccgat cgagtggaag ggcgggcagc cgcgaggctc cccgcccttt ttgtctggga
     3541 gttccccaga acttacgagg ccacgtcgat gcgtcgcata gaccggctga tttccgacgg
     3601 cggatttacg cttgtcgagc tgatcgtgat gctgctcgtg atcgccgtga tcgccgtgat
     3661 cgcggcgccc aagatgagca
//
LOCUS       DASPZD010000265         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_382522, whole genome shotgun sequence.
ACCESSION   DASPZD010000265 DASPZD010000000
VERSION     DASPZD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382522"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VEM33_05150"
     CDS             <1..1683
                     /locus_tag="VEM33_05150"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HYS51242.1"
                     /translation="ANRGSISVDAVLELDVASDFAAMLAIRGAAPGLGAPVPAPAVKT
                     VDGVRFSRDGRSTRVVTFPEGLQHQLRLAPGREFVLTVDVVPDADTAAVDFDTGLGAV
                     RDVYPRWAADCMSVRTDNPVLNDLLEQAIADVRMLCNTYDTGIYPTAGLPWFAVPFGR
                     DTLISSIQLLHVNPELARGVLRYLAKHQGRHVDESSEEQPGRILHEVRSGEVVERGLW
                     PHILYGTIDATPLFLCALTETEHWTYDHRLADELWPAAEAALEWCTTFGDPDGDGYIE
                     HYGGRARNQGWKDSDDSLTNNDGSEVARPAALCEVQAYLQRGLVGMSRRNPALKKDAA
                     RLKRDFNRDFWMPHEKFIAQALDGAKRPVEAITSNPGHCLWMRILDGPRAAHVTSRLT
                     SRDLFSGWGIRTLSDRAVNYDPCSYHNGSVWPFDTALAVAGMRQYGFAAEAERTARAL
                     IEASIGFPMHRPPELFCGDVRTLGEPPKEYWNTCTPQLWSAAAMFTCVASILGLDADP
                     HSKTLRIAPIETGLWNRIEVTGLHFAGERLDFSVDGTQVRPGPMPAGIRITS"
     gene            complement(1676..>2492)
                     /locus_tag="VEM33_05155"
     CDS             complement(1676..>2492)
                     /locus_tag="VEM33_05155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYS51243.1"
                     /translation="RRRAFDVIHDNSGMNGILVAAQARLQAPVVATLHGALSEAEGDF
                     LNAVDRQVHLVSISRAQQALVASVEWRGMVHNAINPEEYTPITKPEEKQDYLVELARI
                     SPDKGQDVAIEIAKRLRMPLVLAGKVDYDARRYFEEKIKPNLDGQVTWREDVRGKEKA
                     ELLARARAMLFPIQWEEPFGIAMVEAMVSGTPVIAMRRGAAPEVVEPGVTGFLAEDLD
                     GMVEAFGRIKEIDLKRCAEVAAGRFGPAQMADGYVNVYERAIDDALYSAPPLS"
BASE COUNT          419 a          844 c          776 g          453 t
ORIGIN      
        1 gccaaccgcg gctcgataag cgtcgacgct gtgctcgagc tggatgtggc gtccgacttc
       61 gccgccatgc tcgcgattcg cggcgcagcg cccgggctgg gagctcccgt tcccgcaccc
      121 gccgtcaaga ccgtcgacgg agtgcgcttc tcgcgtgacg gccgctcgac gcgggttgtc
      181 acttttcccg agggcctgca gcaccagctg cggctcgcac cgggccggga attcgtcctg
      241 acagttgacg tggtgcctga cgcagacaca gccgctgtcg atttcgacac cggccttggc
      301 gccgtccgag acgtataccc gcgctgggcg gccgattgca tgtcggtgcg caccgacaac
      361 ccggtcctca atgatttgct ggagcaggca atcgcggacg tccgcatgct gtgcaacacc
      421 tacgacacgg gcatctatcc aacggccggg ctgccgtggt tcgcggtgcc gttcggccgc
      481 gacacgctga tcagctcgat ccagctcctc cacgtcaacc ccgagctcgc gcgcggcgtc
      541 ctgcggtacc tcgccaagca ccagggcagg cacgtcgacg agagcagcga agaacagccg
      601 ggcaggatcc tccacgaggt gcgctccggc gaggtggtcg agcgcggcct gtggccgcac
      661 atcctctacg gcaccatcga cgccacgccg ctctttctct gtgccctgac tgaaaccgag
      721 cactggacgt acgaccatcg ccttgcggac gagctttggc ctgcggccga agccgcgctc
      781 gagtggtgca cgaccttcgg cgacccggat ggggacggtt acatcgagca ctacggaggc
      841 cgcgcgcgca accagggatg gaaggattcc gatgattcgc tcaccaacaa cgatggaagc
      901 gaggtggctc gtcccgccgc gttgtgcgag gtgcaggctt atctgcagcg cggtctcgtc
      961 ggtatgtccc gtcgtaaccc cgcgctgaag aaagatgcgg ccaggctgaa gcgcgatttc
     1021 aaccgtgact tctggatgcc gcatgaaaag ttcatagccc aggcgctcga cggcgccaag
     1081 cgccccgtcg aagccatcac ctcgaatccc gggcactgcc tctggatgag gatccttgat
     1141 gggcccaggg ctgcgcatgt gacgagtcgc ctcacgtccc gggacctctt ctcgggttgg
     1201 ggaatccgca cgctatcgga ccgtgcggtc aactacgacc catgcagcta ccacaacggc
     1261 tcggtgtggc cgttcgacac agcgcttgcc gtggccggga tgcggcagta cggattcgcg
     1321 gcggaggccg aacgcaccgc acgcgcgctg atcgaggcat caatcggctt cccgatgcac
     1381 cggccgccag agcttttctg tggtgacgtg cgtacactgg gcgaaccgcc caaggagtac
     1441 tggaacacct gcaccccgca gctctggtca gcggccgcga tgttcacgtg cgtggcgtcg
     1501 attctgggcc tggacgccga cccacacagc aagacgctcc gcattgcgcc aatcgaaacc
     1561 gggttgtgga accggatcga ggtgaccggc ctccactttg cgggtgagcg cctggatttt
     1621 tcagtcgacg gcacgcaggt gaggcccggc ccgatgccgg ccgggattcg aatcactagc
     1681 tgagaggcgg ggcgctgtac aacgcgtcgt cgatcgcgcg ctcgtagacg ttcacgtaac
     1741 cgtccgccat ctgtgcggga ccaaaacgcc cggctgcgac ttccgcgcat cgcttcaggt
     1801 cgatctcctt gatcctgccg aaggcttcga ccattccgtc cagatcctcg gcgagaaatc
     1861 ctgtgacgcc cggctcgacc acctcgggtg cggcacctcg gcgcatcgcg atcacagggg
     1921 tgccgctgac catggcttcg accatcgcaa tgccaaatgg ttcctcccat tggatcggga
     1981 agagcatcgc cctcgcgcga gccagcagct ccgccttctc cttgccccgc acgtcctccc
     2041 tccaggtcac ctgtccatcg aggttgggct tgatcttctc ttcgaagtag cgccgcgcgt
     2101 cgtaatcgac cttgcccgca agcacgagcg gcatcctcaa gcgcttggcg atctcgatcg
     2161 cgacatcctg tcctttatcc gggctgattc gcgccagctc gacgaggtag tcctgctttt
     2221 cttccggctt ggtgatcggc gtgtattcct cgggattgat cgcgttgtgg accatgccac
     2281 gccactcgac gctggcgacc agcgcctgct gcgctcggct gatcgacaca aggtgaacct
     2341 gtcgatcgac ggcgttgagg aaatcgccct cggcttccga tagcgcacca tgcagcgtcg
     2401 ctacgacagg agcctgcagg cgcgcctgcg ccgcgaccag gatgccgttc attccagagt
     2461 tgtcgtggat gacgtcgaac gcccgccggc gt
//
LOCUS       DASPZD010000266         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_253083, whole genome shotgun sequence.
ACCESSION   DASPZD010000266 DASPZD010000000
VERSION     DASPZD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253083"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VEM33_05160"
     CDS             <1..1025
                     /locus_tag="VEM33_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51244.1"
                     /translation="LVTAVIGRAARQPAREARMSGKLRRSITALALCAAQSVVWAQAW
                     PAKTVRFIVPFTPGSGTDIMARTVSDKLTAQLGQTVVVENRPGAGGTIGTALAAKAEP
                     DGYTILVHSSSYTVTPSTYQNLPYDTLRDLTGVVPLGLLPNVLVIAPSKSIRSVKELV
                     AMAKAKPGAMNSASVGIGSATHLNAERFRLGAGIDVVNIPFKGTPEALTEVMTGRVDY
                     YFCPVNAVLPFLKDAKVLALAVGSSKRSLALPELPTTLEAGVANSDYNFWVGMFAPAK
                     TPRDVLNRLHRETVKALHLPDVREKLARLGAEPMDYNLEQFNAYLREEIAANAALVKA
                     AGIKAE"
     gene            complement(1042..2199)
                     /locus_tag="VEM33_05165"
     CDS             complement(1042..2199)
                     /locus_tag="VEM33_05165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYS51245.1"
                     /translation="MAKKKLTKIGRYKILGELGRGAMGIVYEAEDPALDRTVALKTII
                     LSDDAEGRKEFHKRFFLEAKAAGKLTHPRIITVYDFGEEGDLAYMAMELLKGIELRAK
                     MLKGPISTVDAVDIAEQVADGLGYAHERGVVHRDIKPSNIMLLPRGQVKIMDFGIARV
                     RVSDHKTSTGVVLGTPRYMSPEQVAGSDVDHRSDIFSLGIVLYEMLTRASLFAGADTP
                     QILHNVVHVEHIPPSRLNREVPSMLDFVIARALKKDPAVRYQDVYELAADLRSCLAEL
                     GGRKHAEKDADGDQDITKTVKLDVDTAKTRLAPAARAIGSDTRLPLSRQFDSSAALGR
                     LSAPTKRDRELLARPPRPVGFFRRLKNDPGPRLLFIAVLAAALVGSAIAFA"
     gene            complement(2181..2909)
                     /locus_tag="VEM33_05170"
     CDS             complement(2181..2909)
                     /locus_tag="VEM33_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51246.1"
                     /translation="MKTPNAAATLASVVFLKIQEFARRPVQEQARLRAQLEAVVAVTT
                     AELAPASRIVLDAADGAAIVVLGDPKGALQLAGRALTATAAGLPLCIGVNHGAVQLAA
                     AGRGDEGLIGDGIAVAASVAEFASPSRLLMSRSFREAMADASPGLESGVFPAGVFTDA
                     GLRTHELFSPDGRVAERRRTRFLAFSVAAVIGLIGAGVAARVSAEGSQKFVDGAVVKI
                     RRAIVVYETYLGGVIERVRWQKRS"
     gene            3014..3529
                     /locus_tag="VEM33_05175"
     CDS             3014..3529
                     /locus_tag="VEM33_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HYS51247.1"
                     /translation="MAELKAVSLAEVSEGIRKSVRPVSILWQAPDTIAFVARGRAHRS
                     EFHADPSDEVMYMIKGEMNLHYLTPEGEEKVALVREGEIIYCPAGVRHSPRFSPDSFL
                     LVLERKRRSDERDRFFWYCEKCHAELYQAVRHVADYREDPVSRVYEEFYSQESRRTCG
                     KCGHVTPKPAG"
     gene            complement(3538..3876)
                     /locus_tag="VEM33_05180"
     CDS             complement(3538..3876)
                     /locus_tag="VEM33_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51248.1"
                     /translation="MRIDPFLEAAWRDLNRRSKILWRLFFGFIPAMALLSYLLNDTLL
                     QGAPFLAIALAWMSAIAWAGSRMASFACPRCSRAFFENWYFFKPLRQNCAHCSLARWA
                     KEMPPPATGS"
     gene            complement(3878..4090)
                     /locus_tag="VEM33_05185"
     CDS             complement(3878..4090)
                     /locus_tag="VEM33_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51249.1"
                     /translation="MDALYKGVMVGGGLNGAIGFGSHMERRDWESAKNSIERTYGRSS
                     RAHRLTLDTLSAAIQSRRMLTPRLAG"
     gene            complement(4296..>4542)
                     /locus_tag="VEM33_05190"
     CDS             complement(4296..>4542)
                     /locus_tag="VEM33_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS51250.1"
                     /translation="GLQKTPLQYLKDNLLVTTSGNWYEPAFLCTLLALGADNILFAVD
                     WPYEPNTAGMEFLKKLSISDADREKIAHRNAERILRM"
BASE COUNT          796 a         1459 c         1496 g          791 t
ORIGIN      
        1 atttggtgac agccgtgatc gggcgcgcgg cgcggcaacc cgcgcgggag gcaagaatgt
       61 ctggaaagct gcgtagatcg atcaccgcac tggcgctgtg cgccgcgcag tcggtagtat
      121 gggcgcaggc ctggcctgcg aaaacggtca ggttcatcgt gccgtttacg cccgggagcg
      181 gaaccgacat catggcgcgc acggtctcgg acaagctgac ggcgcagctt ggccaaacgg
      241 tcgtggtcga gaaccgtccc ggtgccggcg gcaccatcgg caccgctctg gcggcgaagg
      301 ccgagcccga cggctatacg atcctcgtgc attcgtcttc gtatacggtg acgccgtcga
      361 cctaccagaa tctgccttac gacacgctgc gcgatctcac gggagtcgtg ccgctgggcc
      421 ttttgcccaa cgttctcgtg atcgcgccgt cgaagagcat ccgctcggtg aaggagctcg
      481 tcgccatggc gaaagcaaag ccgggcgcga tgaactccgc gtcggtcggt atcggtagcg
      541 ccacacacct caacgccgag cgcttccgcc tgggcgcggg aatcgatgtc gtgaacatcc
      601 cgttcaaggg cacgcccgaa gcgctcaccg aagtgatgac cggtcgcgtc gattactatt
      661 tttgtcccgt gaacgcggtc cttccattcc tgaaggacgc gaaggtgctg gcgctcgccg
      721 tcgggagcag caagcgctcg cttgccctgc ccgagctgcc gaccaccctg gaggcgggtg
      781 tcgcgaactc ggactacaat ttctgggtcg gcatgttcgc gcctgcgaag acaccgcgcg
      841 acgtgctgaa ccgcctccac cgggaaaccg tcaaggcgct gcacttaccc gatgtgcgcg
      901 agaagctggc gcgcctcggc gccgagccga tggactacaa cctggagcag ttcaacgcct
      961 atctgcgcga ggagatcgcc gctaacgccg cgctggtgaa ggcggcgggc atcaaggcgg
     1021 aataaggacg cgcgagcggc gtcacgcgaa cgcgatcgcg ctgccgacga gcgccgcggc
     1081 gagcactgcg atgaacaaca ggcgcggacc gggatcgttt ttgaggcgcc ggaaaaaacc
     1141 gaccggccgc ggaggccggg cgaggagctc gcgatcgcgc ttcgtggggg cggaaagccg
     1201 tcccagcgcc gcggaagagt cgaactgccg cgaaaggggg aggcgcgtgt cggaaccgat
     1261 cgcgcgcgcc gcgggcgcga gccgcgtctt cgccgtgtcc acatcgagct tcaccgtctt
     1321 cgtgatgtcc tgatcgccgt cggcgtcctt ttccgcgtgc ttgcggccgc cgagctcggc
     1381 gaggcagctg cgcaggtcgg cggcgagctc gtagacgtcc tggtagcgta ccgcggggtc
     1441 tttcttgagc gctcttgcaa tgacgaaatc gagcatcgac ggcacctcgc gattcagacg
     1501 cgagggcggt atgtgctcga cgtggacgac gttgtgcagg atttgcggcg tgtccgcgcc
     1561 cgcgaacaac gaggctcggg tgagcatctc gtagagcacg atcccgagcg agaagatgtc
     1621 ggaccggtgg tcgacatccg atcccgccac ctgctcgggg gacatgtatc tgggcgtgcc
     1681 cagcacgaca cccgtgctcg tcttgtggtc ggaaacgcgc acgcgcgcga tcccgaagtc
     1741 catgattttc acctgcccgc gcggcagcag catgatgttg ctcggcttga tgtcgcggtg
     1801 gacgactccg cgctcgtgcg cgtagcccag gccgtcggcg acctgctccg cgatatcgac
     1861 cgcgtcgacg gtcgagatcg ggcccttcag catcttcgcg cggagctcga tccccttgag
     1921 gagctccatc gccatgtagg cgaggtcgcc ttcctccccg aaatcgtaga cggtgatgat
     1981 gcggggatgc gtgagctttc ccgcagcctt ggcttcgagg aaaaaccgct tgtggaattc
     2041 cttgcgccct tccgcgtcgt ccgaaaggat gatggtcttc agcgcgacgg tgcggtcgag
     2101 agccgggtct tcggcctcgt agacgattcc catcgcgccg cggcccagct cgccgaggat
     2161 cttgtagcgg ccgatcttgg tcagcttctt ttttgccatc ggactctttc gatcacgccg
     2221 ccgaggtaag tttcgtaaac gacgatcgcc cgccggatct tgacgacggc gccgtccacg
     2281 aatttctggg aaccttcggc cgacacccgt gccgcgacgc cggccccgat gagcccgatc
     2341 acggccgcga cgctgaaggc gaggaagcgt gtccgccttc gctcggccac ccgcccgtcc
     2401 gggctgaaca gctcgtgggt ccgaagaccg gcgtcggtga aaacccccgc cgggaaaacg
     2461 ccggattcga gcccgggcga agcgtcggcc atggcttcgc ggaaggaccg cgacatcagc
     2521 aggcgcgagg gcgaagcgaa ctcggcgacg ctcgccgcca ccgcgatgcc gtcgccgatc
     2581 aacccctcgt cgccccggcc ggcagccgcc agctgcaccg cgccgtgatt gacgccgatg
     2641 cacaggggca gcccggctgc cgttgcggtg agagcgcgcc cggccagttg cagggccccc
     2701 ttgggatcgc cgagaaccac gatggcggcg ccgtccgcgg cgtcgagcac gatgcggctc
     2761 gcgggcgcga gctccgcggt ggtcacggcc accacggctt cgagctgcgc acgcaggcgc
     2821 gcctgctcct ggaccggcct gcgggcgaac tcctggattt tcagaaagac gacgctagcg
     2881 agggtcgcag ccgcgttcgg tgttttcatt tgccaagaac cgacggtgcc gacgatctta
     2941 ccgtttacct acctcttgcg cttacaatct ccggtgacag cgtaacgatc tcgaactgga
     3001 gcaagagcgc aaggtggcag agctcaaggc cgtcagcctc gcggaggttt ccgagggcat
     3061 caggaagagc gtccggccgg tgagcatcct ctggcaggcg cccgacacca tcgccttcgt
     3121 cgcgcgcggg cgagcgcacc gcagcgagtt ccatgcggat ccgagcgacg aagtcatgta
     3181 catgatcaag ggtgagatga acctgcacta cctcacgccg gaaggcgagg agaaggttgc
     3241 gctcgtccgc gaaggcgaga tcatctactg cccggccggc gtacggcact cgccgcgctt
     3301 ctcgcccgac tcgttcctgc tcgtgctgga gcgcaagcgc cgctccgacg agagggaccg
     3361 cttcttctgg tattgcgaga agtgccacgc ggagctgtac caggcggtcc ggcacgtcgc
     3421 cgactaccgc gaggaccccg tgtcgcgcgt ctacgaggag ttctattcgc aggagtcgcg
     3481 ccgcacctgc gggaagtgcg gccacgttac gccgaagcct gcggggtagc gtgccagcta
     3541 ggatccggtc gccggcggcg gcatttcttt agcccagcga gccaggctgc aatgagcgca
     3601 gttctgcctg agcggcttga agaaatacca gttctcgaag aacgccctgc tgcagcgggg
     3661 gcatgcgaag ctcgccatcc ggcttccggc ccacgcgatc gcgctcatcc aggcgagcgc
     3721 aatagcgaga aacggtgcgc cctggagaag cgtgtcgttc agcagatagg aaagcagagc
     3781 cattgctgga atgaagccga agaacagccg ccagagaatc ttggacctgc ggttcaggtc
     3841 acgccatgcc gcctcgagga agggatcgat gcgcatgtca gcccgcgagc ctgggggtga
     3901 gcatccttct gctctgtatt gcggcactca aggtatccag ggtgagtcgg tgcgcccggc
     3961 tcgaacgtcc ataggtccgc tcgatgctgt tctttgcgct ttcccaatcc ctgcgctcca
     4021 tgtgcgagcc gaaaccgatc gctccgttca gaccccctcc gaccatgacg cccttgtaca
     4081 aggcatccat caggtgatcc agcgcttcgc gggaatgcgc tcgcgatacg cgaatcgccc
     4141 atggttgatc gggatgggaa atgacggtgg cctatctttg agccgaagtt ttgtctctca
     4201 cgcctcatta tgacctactc ggcaggcttc tctgtgcgcc accacacgga gaggacaaaa
     4261 cctttcgatg aaacatgagg aaggcggcgc cggcatcaca tccgcaggat gcgctcggcg
     4321 ttgcggtgcg cgatcttctc gcggtccgcg tcgctgatcg aaagcttctt caggaattcc
     4381 attcccgccg tgttcggctc gtagggccag tcgaccgcga agaggatgtt gtccgcgccg
     4441 agcgctagca gcgtgcacag gaacgcgggc tcgtaccagt tgccgctggt ggtgacgagc
     4501 agattgtcct tcagatactg cagcggcgtt ttttgaaggc cc
//
LOCUS       DASPZD010000267         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1296395, whole genome shotgun sequence.
ACCESSION   DASPZD010000267 DASPZD010000000
VERSION     DASPZD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296395"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..927)
                     /locus_tag="VEM33_05195"
     CDS             complement(28..927)
                     /locus_tag="VEM33_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYS51251.1"
                     /translation="MAARSIASLTVSFGLVSIPVKLFSATEASRAISFNLLHKGCGSR
                     LKQQYICIKEEVPVVREDMVKGYEFAKDQYVMFTPEELKAMEEAGTHMAEITEFVPIE
                     AVDPVYFDKAYYLAPDKGGAKPFALLASALRESKRCALGRWAARGKQYIVMIRPVEEG
                     LVMQQLLYAGEVRSIKDIEIPKTEVKAAELKLAKMLIEQQASDTFDPAVYTDEVSARI
                     EAAVQKKVEGQEITLAEAPEGGGAQVIDLMEALRASLEKKAAAPSKARESQPEARKPP
                     KRAQETPAAAVAPAARKRVKNKA"
     gene            complement(947..>2152)
                     /gene="ligD"
                     /locus_tag="VEM33_05200"
     CDS             complement(947..>2152)
                     /gene="ligD"
                     /locus_tag="VEM33_05200"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYS51252.1"
                     /translation="VKFQNWTDDGSLRAPVFLRFRDDIDAKSVRRAKGPKAASRAPRE
                     EGELEAILRQLDGEKTSLTLAVGTHQIRLTHLERVYWPADAALKQPALTKRDLLRYFA
                     QVSPFILPHLADRPLTMIRMPDGIHGQRFFQKHWEQERPDFVQTITVFSAHKDEQHAY
                     LLCNNLPTLLWLAQSGTLEFHVWHSRAKPGPDTAGKGTDYASSLAALESSVLNYPDYV
                     VFDIDPYIYSGKEAAGAEPELNTVAFEKGKEVAFWLRELLLSMSLEPIVKTSGKTGLH
                     VFVPIRRTIDFDAARHVSELVGRHLMRLHPKDITLEWSVPKRTGKIFMDYNMNVRGKT
                     LNVAYSPRGEPGAPVSMPLTWEELAGAHPLDFRITNAAQRLSETGDRWRDALSRKQSL
                     ESALKLVKA"
BASE COUNT          340 a          691 c          690 g          431 t
ORIGIN      
        1 ggatgcatcg aagcgctgct ttgcgtgcta agccttgttc ttgacacgct tgcgcgccgc
       61 cggcgccacg gcggcggcgg gagtctcctg cgcccgcttg ggcggcttgc gtgcttcagg
      121 ctgcgactcg cgcgccttgg acggtgctgc cgctttcttt tcgaggctgg cgcggagcgc
      181 ttccatgaga tcgatgacct gagcgccgcc gccttcgggc gcctcggcca gggtgatctc
      241 ctgaccctcg actttcttct gcaccgccgc ctcgatgcga gcgctcacct catcggtgta
      301 cacggcggga tcgaaggtgt cggacgcctg ctgctcgatc agcatcttcg ccagcttgag
      361 ctcggccgcc ttcacctcgg tcttcgggat ctcgatgtcc ttgatcgacc gcacttcgcc
      421 ggcatagagg agctgctgca tgacgaggcc ctcttcgacc ggacggatca ttacgatgta
      481 ctgcttgcca cgcgcggccc agcgcccgag cgcgcagcgc tttgactcgc gcaatgcgct
      541 cgcgagcagc gcgaaaggct tggcgccgcc cttgtccggc gcgagatagt acgccttgtc
      601 gaagtaaacc gggtcgaccg cctcgatcgg cacgaattcg gtgatctcgg ccatgtgggt
      661 gcccgcctcc tccatcgcct tcagctcctc gggcgtgaac atcacgtact ggtccttggc
      721 gaattcatag cctttgacca tgtcctcgcg cacgaccggc acttcctcct tgatgcagat
      781 gtactgctgc ttcaagcgcg acccgcagcc cttgtgcagc agattgaagg agattgcgcg
      841 gctggcctcg gtggccgaaa agagtttcac cggtatcgac accagcccga acgaaaccgt
      901 caacgatgct atcgatcgtg cagccatgtc ctgcctccct cgttgttcag gccttgacga
      961 gctttaacgc gctctcaagg ctctgcttgc ggctcagggc gtcgcgccat cgatcgcccg
     1021 tctctgacag gcgctgcgcc gcgttggtaa ttctgaaatc gagcgggtgc gcgcctgcga
     1081 gctcttccca ggtcaagggc atcgacaccg gcgccccggg ttcgcctcgc ggcgaatagg
     1141 cgacgttcag cgtcttgcca cgaacgttca tattgtaatc catgaagatt ttgcctgtcc
     1201 gcttgggcac gctccattcc agggtgatgt ccttggggtg caggcgcatc aggtgcctgc
     1261 ccaccagctc ggacacgtgc cgggccgcgt cgaaatcgat ggtgcgccgg atgggcacga
     1321 acacgtgcag gccggtctta cccgaggtct tgacgatggg ctcgagcgac atgctgagca
     1381 ggagctcgcg cagccagaac gccacctcct tgcccttctc gaaggcgacg gtgttgagct
     1441 cgggctcggc gcccgccgcc tccttgccgg agtagatata aggatcgata tcgaaaacga
     1501 cgtagtcggg atagttcagc accgagcttt ccagcgcggc cagcgagctc gcgtaatcgg
     1561 tgcccttgcc ggcggtgtcc gggccgggct tggcgcgcga atgccagaca tggaactcga
     1621 gcgttccgga ttgcgcgagc cacaggagcg tcgggaggtt gttgcacagc agataggcgt
     1681 gctgctcgtc cttgtgcgcc gaaaaaacgg tgatcgtctg gacgaagtcg ggccgctcct
     1741 gctcccagtg cttctggaaa aagcgctgcc catggatccc gtcgggcatg cggatcatgg
     1801 tgagcggacg atcggccaga tgcggcagga tgaagggcga cacctgcgcg aaatatcgca
     1861 gcaggtcgcg cttggtcagc gccggctgct tgagcgccgc gtcggccggc cagtagacgc
     1921 gctcgaggtg cgtgagccgg atctggtgcg tgccgacggc gagcgtgagc gatgtcttct
     1981 cgccgtcgag ttgacgcagg atggcctcga gctctccctc ctcccttggc gcacggctcg
     2041 ccgcctttgg acccttggcg cggcgcacgc ttttggcgtc aatgtcgtcg cggaagcgca
     2101 ggaacacggg cgcgcgcaga gagccgtcgt cggtccagtt ctggaacttc ac
//
LOCUS       DASPZD010000268         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_725539, whole genome shotgun sequence.
ACCESSION   DASPZD010000268 DASPZD010000000
VERSION     DASPZD010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725539"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VEM33_05205"
     CDS             complement(<1..1151)
                     /locus_tag="VEM33_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS51253.1"
                     /translation="MDLIADRGRRVAIEELNTTRLLAHARKQAQQRNLDDILIVDVDA
                     HHYENECLDEFLPFMENDVLRQLTLGGRGKGRGSIVPTNAVGFQEMGGRITRYPMRSS
                     EKTTPGKLRDVELGHRWMDALSVDYSCLFPTLMLAVGLHPDPEMEVQLCWAYNRWLTE
                     KALPEGGGRFYSMLTLPLSDPDAALRQIETFGARKYVTGFMITCVRSLAVHHNAHMKI
                     YRALEERGLVLSFHSAINPGEPVFRNLNRFASVHALGFPFYNILHMTNWVTNGLAERF
                     PKLPVIWIEGGIAWVPFLMQRLDHEYMLRSSEYPLLKKKPSDYMRGMYYASQPMEADN
                     LRALACTFEMINAETQLLYASDYPHWDFDLPSRIWDLPFLSEKAKHNILG"
     gene            complement(1299..1850)
                     /locus_tag="VEM33_05210"
     CDS             complement(1299..1850)
                     /locus_tag="VEM33_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYS51254.1"
                     /translation="MNLSPAGFPALVLNADFRPLSYYPLSLWSWQDAIKAVFLERVNI
                     VAEYDRAVRSPSLEMKLPSVVSLKTFVKPSTHPAFTRFNVFLRDKFVCQYCGSREDLT
                     FDHLLPRSRGGHTTWINVVAACSPCNLKKGNLTTHEAKMWPSQMPFQPTVNHLHRNGR
                     RFPPNYLHESWLDYLYWDSELQP"
BASE COUNT          401 a          615 c          748 g          398 t
ORIGIN      
        1 ccgaggatgt tgtgcttggc cttctccgac agaaacggca ggtcccagat tcggctgggc
       61 agatcgaagt cccaatgggg ataatccgac gcgtaaagga gctgggtttc cgcgttgatc
      121 atctcgaagg tgcacgcgag cgcgcgcagg ttgtcggcct ccatcggctg cgaggcgtag
      181 tacatgccgc gcatgtagtc gctcggcttc ttcttcaaca gcggatactc ggagctgcgc
      241 agcatgtatt cgtggtcgag gcgttgcatc aggaacggga cccacgcgat cccgccctcg
      301 atccagatca cgggcagctt ggggaagcgt tcggcgagcc cgttggtgac ccagttggtc
      361 atatgaagga tgttgtagaa cgggaagccg agcgcgtgca cggaggcgaa acggttgaga
      421 ttcctgaaca cgggctcgcc cggattgatg gcggaatgga aggagagcac gaggccgcgt
      481 tcttccaagg ctcggtagat cttcatatga gcgttgtgat gcaccgccag gctgcgcacg
      541 caggtaatca taaagccggt cacgtatttg cgcgccccga aggtctcgat ctgacgcagc
      601 gccgcgtccg ggtccgacag cggcaacgtc aacatcgagt agaagcggcc gccgccttcg
      661 ggcagcgctt tttcggtcag ccatcgattg taggcccagc acagctggac ctccatttcg
      721 ggatcggggt gcagcccgac cgcgagcatc aaggtcggga acaggcagga atagtcgacg
      781 ctcaacgcgt ccatccagcg atggccgagc tcgacgtcgc gcagcttgcc gggggtggtc
      841 ttctccgatg agcgcatggg gtagcgcgtg atgcgcccgc ccatctcctg aaagccaacc
      901 gcattggtgg gaacgatgct gccacggccc ttgccgcggc cgccgagcgt gagctggcgc
      961 aggacgtcgt tctccatgaa aggaagaaac tcgtccaagc actcgttctc gtagtggtgg
     1021 gcatcgacgt cgacgatcaa gatgtcgtcg aggttgcgct gttgcgcctg cttgcgggcg
     1081 tgggcgagca gccgggtggt gttgagctct tcgattgcga cgcgtcggcc gcgatcggcg
     1141 atgaggtcca tggcgcttct cccgacggtg gatgatcggc ccaagtgtat tcgcgtcgcg
     1201 gtcgccgcga aagcgccttg agcggtgttc gggcgcagag cgacgcggag cgttgagctt
     1261 ccgcgcacga cgataatcgc aacggtacgc gagcgcgctt acggctgcag ttcgctgtcc
     1321 cagtagagat agtcgagcca gctctcgtgc agatagttcg gcgggaagcg ccggccgttg
     1381 cggtgtaaat gattgacggt aggctggaac ggcatctgcg acggccacat cttggcctcg
     1441 tgtgtcgtca ggttgccctt cttgagatta cagggcgagc aggccgcgac cacgttgatc
     1501 caggtcgtgt gcccaccgcg cgagcgcggc aatagatgat cgaaggtgag atcttcgcgc
     1561 gagccgcaat actggcagac gaacttgtcg cgcaggaaaa cgttgaagcg ggtgaaggcc
     1621 ggatgggtcg agggcttcac gaaggtcttg agcgaaacca cgctggggag cttcatctcc
     1681 aagctcgggc tgcgcaccgc gcggtcgtac tcggcgacga tattcacccg ctcgagaaag
     1741 accgccttga tcgcgtcctg ccacgaccac agcgacagcg ggtagtagct gagcggacga
     1801 aaatccgcgt tgagcacgag ggcgggaaag ccggcggggg ataagttcac ggcgctcctt
     1861 gcctcctttc cgtgcgaacc gcagcggtgg ccacagtcta taggggagca tgacgggatt
     1921 gtgaagcctg atccgggggt tatgtggggc cggagggcgc ggccgaagtt gctccgacgc
     1981 aggcgagtta atctcgcgcg acgctgcttg gcactggccc tgcgcttgga gtcctaggcc
     2041 ccgcgcacgc gatcgatcac ggccgttgca tcggcgacgg cgtcgccgtg ccaggcgatg
     2101 tgctgatcgg gacgaatcag cacgagcttg cggcggtaga ggccacgggg cgattcgagc
     2161 cg
//
LOCUS       DASPZD010000269         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_969079, whole genome shotgun sequence.
ACCESSION   DASPZD010000269 DASPZD010000000
VERSION     DASPZD010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969079"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1493
                     /locus_tag="VEM33_05215"
     CDS             132..1493
                     /locus_tag="VEM33_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYS51255.1"
                     /translation="MDATTRRLVDFALAYRFEDLPASTVHAAKARLLDSAAVGLAAYA
                     APPVRILRKCAPRVEGPWAATLWGTRDKTTPDHAALVNGTMVRYLDLNDAYRTLDASH
                     PSDNLPGLVAAAQALGSSGRELILALFISYEIQCRFTDTVPFNAAGWDQPVVGAQAAA
                     LAVGRMMGLDAEQLAHALSLSVVPHLCTYQTRSGELSMWKGCAGPNGARHGVFAAQLA
                     REGLTGPYSAYEGIFGIWRQTLGKPGEFKLPAGRLAAPLGLNQTNIKKYPVRDSCQLP
                     IDTALELRKKLAGRQPKSLKVDTYKSAHLGAVADPELWAPKTRETADHSMPFSIAAAL
                     ADGEVTSDTFENHRFLDTDVLGLIARMKVEINADFSRQTPGLRHCRIEARLESDEAMV
                     AQCSLALADIEKGTPDEVLNAKFHTLTRRFLPEPARNELAAAIWRLETSERLDPLIDL
                     MRI"
     gene            1498..2178
                     /locus_tag="VEM33_05220"
     CDS             1498..2178
                     /locus_tag="VEM33_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HYS51256.1"
                     /translation="MTTRDVKSDAARLLNTVAQGGVGIFATDVGYAIIGNGEAAIARI
                     FACKQRNFDKACGMFSNWDMFDALAQVGQRERKIVDTVIHEHGLPLSVVTPYKIGHPF
                     FSRLTPLTRERSSRGDTIDMLLNAGALHDEIARLAWSRGVPLLGSSANQSLSGSKYKL
                     ADVEQAVRDEADLVIDYGDTKYSHPAGMGSSIIALPSLKPIRKGIKFDEICSLIAQCF
                     GTDPRAVT"
BASE COUNT          564 a         1138 c          994 g          507 t
ORIGIN      
        1 cgcgggatgc gcggcctgcc gctcggcgtg caactcgtcg gcggtccgga ggctttgctc
       61 ttcgcggccg cgagctggag cgcccggcgt ttgcatgaag ccacactagg agaacgcgca
      121 tgacccccac gatggacgct accactcgcc ggctcgtcga cttcgcgctc gcttaccgct
      181 tcgaggacct gcccgcttcc acggtccacg cggcgaaggc gcgcctgctc gattctgccg
      241 cggtcgggct ggccgcctac gccgcgcccc cggtgcgtat tcttcgcaag tgcgcgccgc
      301 gtgtcgaagg gccctgggca gccacgcttt ggggcacgcg cgacaagacg actccggacc
      361 atgccgcgct cgtgaacggc acgatggtgc ggtatctcga cctcaacgac gcgtatcgca
      421 cgctggacgc ctcgcacccg tccgacaacc tgccggggct ggtcgcggcg gcccaagcgc
      481 taggcagctc cggccgcgag ttgatcctcg cgcttttcat ctcctatgag atccagtgcc
      541 gcttcaccga cacggtgccg ttcaacgcgg cgggctggga ccagccggtg gtcggtgcgc
      601 aagccgccgc gctcgccgtc ggccgcatga tggggctcga cgcagagcag ctcgcgcacg
      661 cgctctcgct gtcggtcgta ccgcacctgt gcacctatca gacgcgctcg ggcgagctgt
      721 cgatgtggaa aggctgcgcc ggccccaacg gcgcccgcca cggtgtcttc gcggcgcaac
      781 tggcgcgcga ggggctcacc gggccctaca gcgcttacga gggaatattc gggatctgga
      841 ggcagacgct cggcaagccc ggcgagttca agctgccggc gggcaggctc gccgcgccgc
      901 tcggcctgaa ccagaccaac atcaagaaat acccggtgcg cgactcgtgc cagctgccga
      961 tcgacacggc gctcgagctg cgcaagaagc tggccggccg gcagccgaag agcctcaagg
     1021 tggacacgta caagtcggcc cacctgggcg cggtcgccga tccggagctc tgggcgccga
     1081 agacgcgcga gaccgccgat cattcgatgc cgttctcgat cgccgcggcg ctggccgatg
     1141 gcgaggtgac cagcgacacc ttcgagaacc atcgcttcct ggacaccgat gtcctgggcc
     1201 tgatcgccag aatgaaagtc gagatcaacg ccgatttctc gcgccagaca cccggtctgc
     1261 gccactgccg catcgaagcg cgcctcgaga gcgacgaggc aatggtcgcg caatgctcgc
     1321 tcgcgctggc cgacattgaa aaagggacgc ccgacgaggt cctgaacgcc aagttccaca
     1381 cgctcacccg gcgtttcctg cccgaacctg cgcgtaacga gcttgccgcc gccatatggc
     1441 ggctcgagac gtccgagcgc ctcgatccgc tcatcgacct gatgcggatc tgagcccatg
     1501 accacccgcg atgtgaagtc cgatgccgcc cgcctgctca acaccgttgc gcagggcggg
     1561 gtgggaatct tcgccaccga tgtcggctac gccatcatcg gcaacggcga agccgcgatc
     1621 gcgcgcattt tcgcctgcaa gcagcgcaac ttcgacaagg cctgcggcat gttcagcaac
     1681 tgggacatgt tcgatgctct cgcgcaggtc gggcaacgcg agcgcaagat cgtcgacacc
     1741 gtgatccacg agcacggcct gccgctctcg gtggtgacgc cgtacaagat cggccatccg
     1801 ttcttctcgc ggctcacgcc gctcacccgc gagcgctcct cgcgcggcga tacgatagac
     1861 atgctgctga acgccggcgc gctgcacgac gaaatcgcgc gcctcgcctg gtctcgcggc
     1921 gtcccgctgc tcggctcctc agcgaaccag tccctctcgg gtagcaagta caagctcgcc
     1981 gacgtggagc aggcagtgcg cgacgaagca gacctagtca tcgattacgg agacacgaag
     2041 tactcgcacc ccgccggcat gggaagctcg atcatcgccc tgccctcgct gaagccgatc
     2101 cgcaagggca tcaagttcga cgaaatctgc agcctgatcg cgcagtgctt cggcaccgat
     2161 ccgcgcgccg tgacgtaagc cgggggcccc cggccgccga acgcgcgccc gcacttcatg
     2221 cccttgcttg aaacccacgg gccgggaggc gacctcccac tcgccttccg catcggtgaa
     2281 ccgatcgccg acgcggagct ggttcgggaa gaccgagagc gtcggctccc aacgtgagcg
     2341 ccgtggcacc gccattagtt catccagccc gcccgccgga tcttcttttt ctcgcggagt
     2401 gagggtccga caggaaccct tgagctttgg tttgagatct gcttttcttc ggcagcccat
     2461 cgaaacgccg aggagggtat ccatgcccac accattgtcc gccgaagcca tggcgctcat
     2521 cgaccgccca aatttcgcgc acctcgccac cctgatggcg gacggctcac cccacatcgc
     2581 tcgggcgacc ggcggatccg cttccacgac ctgcgccaca cgtacaccag cctgctcatc
     2641 gcgcagggcg cccatcccaa gtacatccag gcgcagctcg gccacgcctc catccagacg
     2701 acgctcgatc gctacgggca tctgatgccg gacgttcacg cagcggaggc gaagaagctg
     2761 gaccagctcg atctcggtcg gccggcgaca tccgacgacg acagcgcgag cgttaacgcg
     2821 atgcgccggt agcaaaatgg gagcagaaac gacgaagggg ctggcgcggt gcccgccaac
     2881 cccttgatcc cactggttgc ggggctggat tcgaaccagc gacgtttggg ttatgagccc
     2941 gccctagcac gtaactccag ccaaagccac ccaactacaa ccgtcccctg cctcgcgttt
     3001 acgacacgac ggcttcggtg ttcttgggcg gtattggggc cagttcacgt ccagaacacg
     3061 cccaaggccg tggccgcgaa cacgggcgcc gatgatgcgc cagccgtcga ctttttcctc
     3121 atagatccag ccgtcagctg tggcgagcat cgggagacga gactggcgta cgacatcagc
     3181 gggcatcctc ccgcgacggt cga
//
LOCUS       DASPZD010000270         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1332551, whole genome shotgun sequence.
ACCESSION   DASPZD010000270 DASPZD010000000
VERSION     DASPZD010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332551"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VEM33_05225"
     CDS             complement(<1..1430)
                     /locus_tag="VEM33_05225"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HYS51257.1"
                     /translation="MLPERPIAILIAALGGEGGGVLADWIIAAATARGYPVQSTSIPG
                     VAQRTGATTYYVEIYPAEISELGARRPVMTLTPAPCHVDVMVASELLEAGRAMQNGFV
                     TRDRTTLIASTHRVYTVAEKMQMGDGRFDSEVVLKAARELAKRAILFDMQAQASASGT
                     VISAVMFGALAGSGTLPLSRADCEAAIRSGGKGAEASLRGFAGGFDAAAGSAPGKAAM
                     PAERKGLSASERVRQTFPEETRAMLEEGATRCAGFQDRGYARLYLDRLEPIVNLDDAA
                     GGYQLTKETGRFLALWMCYEDVIRVADLKTRRSRFDRVRGEVQAKPHEPVRIIEFLRP
                     GVDEFTAVLPRFLARRVKALAEVSGLARKLNIGMHVKTTSVSGFLALRFLAWLRPLRP
                     FTSRWHEEQMLIGRWLAAIATAAKRDVGLAFEIALCGRLIKGYGETHQRGKDSFLRIL
                     DTLVEGDAVPDGRARTDAIRKAREAAL"
     gene            complement(1433..>2131)
                     /locus_tag="VEM33_05230"
     CDS             complement(1433..>2131)
                     /locus_tag="VEM33_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYS51258.1"
                     /translation="VTIMGDGGFWHNGLTSGIANAVFNKSDGILVIMKNGYTSATGTQ
                     SIPSSLHQTEWKSGDMSIERALKGIGVEWIKPVYTYRVSEMAKTLREAMSSAYKGLKV
                     IIADAECQLERQRRIRPQIAAALKAGKRVVRTRFGVDEDVCSGDHSCIRLSGCPSLTV
                     KAAGDPLKVDPVAHVNNDCVGCGLCGEVAHAAILCPSFFRAEIVQNPNFLDRFAARLR
                     NTVIGWLQPDEERR"
BASE COUNT          328 a          725 c          704 g          374 t
ORIGIN      
        1 agcgccgcct cgcgcgcctt gcggatcgcg tcggtccggg cgcggccgtc cggcaccgcg
       61 tcgccctcca cgagtgtgtc gagaatcctc aggaagctgt ccttgccgcg ctgatgcgtt
      121 tcgccgtagc ccttgatgag cctgccgcac agcgcaattt cgaaggcgag accgacatcc
      181 cgtttcgcgg cggtagcgat cgcggccagc cagcggccga tcagcatctg ctcctcgtgc
      241 cagcgcgagg tgaaaggccg cagcggccgc agccaggcga ggaaacgcag ggcgagaaat
      301 ccgctgacac tggtcgtctt gacgtgcatg ccgatgttga gcttgcgggc gaggccgctc
      361 acctcggcca gggccttcac ccgccgtgcg agaaagcgcg ggagcactgc agtgaactcg
      421 tcgacaccgg gcctcaggaa ctcgatgatg cgcaccggct cgtgcggctt cgcctgaacc
      481 tccccgcgaa cgcggtcgaa gcggcttcta cgcgtcttca ggtcggcgac gcggatcacg
      541 tcttcatagc acatccacag tgcgagaaaa cgcccggttt ctttggtgag ctggtatccg
      601 cctgcagcgt cgtcgagatt cacgatcggc tcgagacgat ccaggtacag cctcgcgtac
      661 ccgcggtcct ggaacccggc gcagcgcgtg gcgccctctt cgagcatggc gcgagtttcc
      721 tccgggaagg tctgcctgac gcgttccgag gcgctcagac ctttcctttc tgcaggcatc
      781 gcggccttgc ccggcgccga cccggccgcg gcgtcgaatc cccccgcaaa gcctctgagg
      841 ctcgcttcgg cgcctttgcc gcccgaacgg atcgccgcct cgcaatcggc gcgcgacagc
      901 ggcagcgttc cggaaccggc caaagccccg aacatcactg cgctgatcac ggtgcccgaa
      961 gcgctcgcct gcgcctgcat gtcgaacagg atcgcgcgct tcgcgagctc ccgcgccgcc
     1021 ttgagcacga cctcgctgtc gaatcgcccg tcgcccatct gcatcttctc ggcgacggtg
     1081 tagacgcggt gagtcgaggc gatcagcgtg gtccggtcgc gcgtcacgaa gccgttttgc
     1141 atcgcgcgtc cggcctcgag cagctcggaa gcgaccatga cgtctacgtg gcagggcgcc
     1201 ggtgtgagcg tcatgaccgg ccgcctggcg ccgagctcgg agatttccgc cggatagatt
     1261 tcgacgtaat aggtggtggc accggtgcgc tgcgccacgc cgggaatcga ggtgctctgc
     1321 accggatagc cgcgcgcggt cgcggcggcg atgatccagt cggccagcac gccgccgcct
     1381 tcgccgccga gagcggcgat caggatcgcg atgggacgtt cgggcagcac gcttaccttc
     1441 tttcttcgtc cggctggagc cagcctatga cggtgttgcg cagtcgtgcg gcgaaccggt
     1501 ccaggaagtt cgggttctgc acgatctcgg cgcggaagaa cgaggggcac aggatcgccg
     1561 cgtgggcgac ctcgccgcac agcccgcatc cgacgcagtc gttgttcacg tgcgcgaccg
     1621 ggtcgacttt gagcggatcg ccagccgcct tgaccgtgag cgaggggcag ccggagaggc
     1681 ggatgcagga atggtccccc gagcacacgt cctcgtccac gccgaagcgc gtgcgcacca
     1741 cgcgcttgcc cgccttgagg gccgcggcga tctgcgggcg gatcctgcgc tggcgctcga
     1801 gctggcactc ggcatcggcg atgatgacct tcagcccctt gtaggcggag ctcatcgctt
     1861 cgcgcagcgt cttcgccatc tcgctgacgc ggtaggtgta caccggcttg atccactcga
     1921 cgccgattcc cttcagcgca cgctcgatgc tcatgtcgcc ggatttccat tcggtctggt
     1981 gcagcgagga agggatgctc tgcgtgccgg tcgccgaggt gtagccgttc ttcatgatga
     2041 ccaggatccc gtcgctcttg ttgaacaccg cattggcgat gcccgaggtg aggccgttgt
     2101 gccagaaacc gccgtcgccc atgatggtga c
//
LOCUS       DASPZD010000271         3424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1537247, whole genome shotgun sequence.
ACCESSION   DASPZD010000271 DASPZD010000000
VERSION     DASPZD010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537247"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VEM33_05235"
     CDS             <1..1041
                     /locus_tag="VEM33_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYS51259.1"
                     /translation="LKDARHVAVKLRNGGTGELEGRRIVINTGCRPAVPTIAGLDRVP
                     FLDSSSIMELTELPRHLIVLGGGYVGLEFCQMFRRFGARVTVVNRDPRLILREDPDVS
                     AEVEKILEEDGIEIINSTQVTLVEKIKAGVRVVLGAGKGSKKISGSHLLLALGRVPNS
                     DRLNLGAAGVRTDKLGYIEVNAKLETNVPGIYAIGDVKGGPAFTHISYDDFRILRENW
                     LNGGNATIEGRPVPNCMFIDPQLATVGLNETDAQKRGIDYRVAKLPMTGVARALEMSE
                     TRGFMKAIVDAKTQQILGCTVLGVEGGEIMSMMQIAMMGKLPYPAIKEAIFAHPTLAE
                     SLNNLFMTLDPR"
     gene            complement(1052..1276)
                     /locus_tag="VEM33_05240"
     CDS             complement(1052..1276)
                     /locus_tag="VEM33_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51260.1"
                     /translation="MRSHEVIALAAILAAGTGAWGVEAPGKPMKMDEPMHGEMKKKGM
                     KKGDVKKAAEKKGRGMKPMAEKESSGAAKK"
     gene            complement(1276..2019)
                     /locus_tag="VEM33_05245"
     CDS             complement(1276..2019)
                     /locus_tag="VEM33_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYS51261.1"
                     /translation="METSLADQPPRLIRAIERRLFLKQGLSLGALALLSGCELSDDEG
                     VQSLLMGMSRWNDRAQGWLFNPNKLAPEYPESAITKPFPFNAFYAESEAPLVDGKDYK
                     LELEGLIREKKPWTLRELYALPQTSQITRHICVEGWSAIGKWSGVRFSDFLARVGADT
                     TAKYIGFECADDYSTSIDMATALHPQTLLTFRFADQILPPKYGFPMKLRMPTKLGFKN
                     PKHITAMFVTNEYPGGYWEDQGYNWFSGS"
     gene            complement(2031..2651)
                     /locus_tag="VEM33_05250"
     CDS             complement(2031..2651)
                     /locus_tag="VEM33_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HYS51262.1"
                     /translation="MEAAQAQWVKVHPLIVRITHWINAFGILIMVASGWRIYNASPLF
                     PFRIPNELTLGGWLAGALQWHFAAMWLLVINGIVYVTYGIVSGHFRRKFFPLTPAAVF
                     RDVLGALRGKLAHEDLSVYNAAQRAAYLAIILCLVVLVFSGLAIWKPVQLQEITATFG
                     GYESARVVHFLAMALLVLIVAVHVVMVILVPRTFPTMITGHARRAS"
     gene            complement(2705..>3424)
                     /locus_tag="VEM33_05255"
     CDS             complement(2705..>3424)
                     /locus_tag="VEM33_05255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYS51263.1"
                     /translation="HLLERVAWLNRLPPVFSGVVLGNEVLDAMPAYVVRVQSGKVEEG
                     GVGVRNDRLGWSWRLATGGPLAAARTLELPEGYRTEIQHAAQGFMRSLSGVLEKGAAL
                     FVDYGFPQKEYYHPQRKDGTLMCHYRHHAHADPFFLPGLQDITTHVDFSAIAGAAREG
                     GLEVAGYASQAQFLVNCGITEVMSRTPAEDGARFLPLANQANRLMSPAEMGELFKVIA
                     FTRGLSQTLLGFGKGDRRSAL"
BASE COUNT          644 a         1077 c         1077 g          626 t
ORIGIN      
        1 cttaaagacg caaggcacgt cgccgtgaag ctaagaaatg gcggcacggg ggagcttgaa
       61 ggcaggcgca tcgtcatcaa caccggctgc cggcctgccg tgcccacgat cgccgggctg
      121 gaccgggtac ccttcctgga ttcctcgagc atcatggagc tcaccgagct gcccaggcat
      181 ctgatcgtgc tcggcggcgg ttacgtcggc ctggagttct gccagatgtt tcgcaggttc
      241 ggtgcgcggg tgacggtggt gaaccgcgat ccccggctga tactgcgcga agaccccgat
      301 gtgtcggccg aggtcgagaa aatcctcgag gaagatggaa tcgagattat caactcgacg
      361 caggtgacgc ttgtcgaaaa gatcaaagcg ggcgtgcgtg tggtgctcgg ggctggcaag
      421 ggatccaaga agatctcggg cagccacctg ctcctcgcgc tggggcgcgt gccgaattcc
      481 gatcgcctga atctcggcgc cgcgggcgtc cggaccgaca agctcgggta catcgaagtg
      541 aatgccaagc tcgagaccaa tgtcccgggc atctacgcga tcggcgacgt gaaaggcggg
      601 ccggccttca cgcacatctc ctacgacgat ttccgcatcc tgcgcgagaa ctggctgaac
      661 ggcggcaacg cgaccatcga agggcgaccg gtgcccaatt gcatgttcat cgatcctcag
      721 ctcgcgaccg tagggctgaa cgaaaccgac gcgcagaaac gcggtatcga ctaccgcgtg
      781 gcgaagctgc cgatgacggg cgtggcgcgc gcgctggaga tgagcgagac gcgcgggttc
      841 atgaaggcga tcgtggacgc aaaaacgcaa cagattctcg gctgcaccgt tctcggcgtc
      901 gaaggcgggg agatcatgtc gatgatgcag atcgcgatga tggggaagct tccctacccc
      961 gcgatcaagg aggcgatctt cgcgcaccct acgctcgccg aatcgctgaa caacctcttc
     1021 atgacgctcg acccgcgcta gcgcgactcg cctacttctt tgccgctccg gacgactcct
     1081 tttcggccat cggcttcatc cctcgccctt tcttctcggc ggctttcttc acgtcgccct
     1141 tcttcatgcc ttttttcttc atttcgccgt gcatgggctc gtccatcttc atgggcttcc
     1201 cgggcgcttc cacgccccag gcgccggtgc ctgccgcaag gatcgccgcc agcgcgatga
     1261 cttcatgcga tctcatcagc tgccgctgaa ccagttgtag ccctggtctt cccagtagcc
     1321 tcccggatat tcattggtga cgaacatcgc cgtgatgtgc ttcggattct tgaagccgag
     1381 cttggtcggc atgcgcagct tcatcgggaa gccgtacttc ggcggcagga tctgatcggc
     1441 gaaacggaaa gtgagcagcg tctgcgggtg caaggccgtc gccatgtcga tgctcgtcga
     1501 atagtcgtct gcgcactcga acccgatgta ttttgccgtg gtgtctgcgc cgacacgcgc
     1561 gaggaaatcg gaaaagcgca cgccgctcca cttgccgatc gcgctccagc cttcgacgca
     1621 gatatggcgc gtgatctggg aagtctgcgg cagggcgtag agctcgcgca gcgtccaggg
     1681 tttcttctcg cggatcaggc cctcgagctc cagcttgtag tccttgccgt ccaccagtgg
     1741 ggcctcgctc tcggcgtaga aagcgttgaa agggaaaggc ttggtgatcg cgctctcggg
     1801 atattccggc gcgagcttgt tcggattgaa gagccagccc tgagcgcggt cgttccagcg
     1861 cgacatcccc atgagcaggc tctgcacccc ttcgtcgtcg ctcagctcgc agcccgagag
     1921 cagcgcgagc gccccgagcg agaggccttg cttcaggaac agccgccgct cgatcgcgcg
     1981 gataagcctc ggtggctgat cggcgaggct cgtttccatc gtccgtttct tcatgacgcc
     2041 ctcctggcgt ggcccgtgat catcgtggga aaagtgcgcg gcacgagaat caccatcacg
     2101 acgtgcaccg ctacgatcag aaccagcaga gccatcgcga ggaagtgcac cacgcgcgcg
     2161 ctttcataac cgccgaaagt cgccgtgatc tcctgcagct gaaccggctt ccagatcgcc
     2221 agccccgaaa agacgagaac gacgaggcac aggatgatcg cgaggtaggc tgcgcgctgc
     2281 gcggcgttgt acacggaaag atcttcgtgc gcgagcttgc cgcgcagcgc ccccagcacg
     2341 tcgcgaaata ccgccgcggg agtcaggggg aaaaactttc tgcgaaagtg tccggagacg
     2401 atgccgtaag tcacgtatac gatgccgttg atcaccagca gccacatcgc ggcaaagtgc
     2461 cactgcagcg cgccggcgag ccagcctccg agcgtgagct cgttcgggat cctgaacggg
     2521 aaaagcggcg acgcgttgta gatgcgccag ccgctcgcaa ccatgatgag aatgccgaac
     2581 gcgttgatcc agtgtgtgat gcgcacgatc agcgggtgga ccttgaccca ttgcgcttgc
     2641 gcagcttcca tcggagtctc ctgtagcgtc cggccccttc tggtcggagg ggataggtta
     2701 caccttacag cgcacttcgc ctgtcgcctt tgccgaagcc gagcaaggtc tgcgagaggc
     2761 cccgcgtgaa agcgatcacc ttgaagagct cccccatttc cgccgggctc atcaggcggt
     2821 tcgcctggtt ggcaagggga aggaacctcg cgccgtcctc cgccggcgtg cgcgacatga
     2881 cctcggtaat gccgcaattg acgaggaact gcgcctggct cgcgtagccg gcgacttcga
     2941 gcccgccctc gcgcgcagct ccggcaatgg cgctgaaatc gacgtgggtg gtgatgtcct
     3001 gcaggccggg gaggaaaaac ggatcggcgt gcgcatggtg gcggtaatgg cacatgagcg
     3061 tcccatcctt gcgctgcgga tggtagtact ccttctgcgg aaagccgtaa tcgacgaaaa
     3121 gcgccgcgcc tttctccagc acgcccgaga gtgaacgcat gaagccctgc gcggcatgct
     3181 ggatctcggt gcgataaccc tcgggcagct cgagcgtgcg cgcggcagct agcggcccgc
     3241 cggtagcaag ccgccaggac cagccgagac gatcgtttcg cacaccgaca ccgccttcct
     3301 ccacctttcc gctttggacg cgcaccacgt aggcaggcat cgcatcgagc acttcattgc
     3361 cgagcacgac gccggagaac acgggaggca ggcggttgag ccaggctacg cgctcgagca
     3421 ggtg
//
LOCUS       DASPZD010000272         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_964041, whole genome shotgun sequence.
ACCESSION   DASPZD010000272 DASPZD010000000
VERSION     DASPZD010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964041"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VEM33_05260"
     CDS             complement(<1..1391)
                     /locus_tag="VEM33_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYS51264.1"
                     /translation="MPAPQSSPDPAESARVFAEVAERSGRIMGEFLQRQAGGQGVAYS
                     DEFGVTRAFMDLAARMLADPWKLAEMQTKMFWDHVALWQSGLMRMLGKEVPAVAEAAK
                     GDNRFRDAGWQDQFLFDYIKQSYLIAARHLHDTVSSVEGLPEETRKKVNFYTRQYIDA
                     LSPSNFALTNPQVLRETLHSGGQNLLKGLNNLLADIERGGIRMADEKAFKLGVNIGTT
                     PGKVVFQSELMQLIQYAPRTEAVFKRPLLVIPPWIIKYYILDLREKNSFVRWAAEQGH
                     TVFVISWVNPDGRYAGKTFENYMFEGPLAALDAIGQATGENEVNIIGYCLGGTLLGAT
                     LAWMAQKNDRRAASATFFASLLDFSIPGELGVFIDEAQVENLERRMQERGYLEGSEMA
                     ATFNTLRANDLVWSFVVNNYLLGKDPFPFDLLYWNSDSTRMPAKMHSFYLRNMYLANR
                     LKEPRGIALAGVP"
     gene            complement(1396..2427)
                     /locus_tag="VEM33_05265"
     CDS             complement(1396..2427)
                     /locus_tag="VEM33_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYS51265.1"
                     /translation="MTAESILEYPHAAPPVPGETLQVAPGVHWLRMPLPFALDHINLW
                     LLEDGEGWTFIDCGIGDDATRALWERIFAGRIAGRPVGRLVVTHHHPDHAGLASWLTE
                     RTGAELWMPQAEYFAAHAMREEGAGFGFDNVVAMFVRNGLSGEKLELMKQRRTNYRSR
                     VPGFPSAYRRLMEGQAHRIGGRDWKVVMGYGHAPEHAALYCEELGVLISGDMVLPRIS
                     TNVSVWANEPEGNPLALFLDSISRYAGLPATTLTLPSHGLPFRGLRERIAQLKEHHRL
                     RLAELHEACETPRAAADVLDTLFRRKLDTHQLFFAMGEAMAHLHLLHYDGRLKHSVGA
                     DGVVRYVQN"
     gene            2552..2959
                     /locus_tag="VEM33_05270"
     CDS             2552..2959
                     /locus_tag="VEM33_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYS51266.1"
                     /translation="MNEPAETYSIADLAREFDVTHRAIRFYEDEGLLSPARDGTRRVY
                     SKRDWVRLKLILRGKRLGFSLAEVHEMLELYDSAPDEGPQLEKFVAALAARREQLEHQ
                     REEIEEVLSEIRAFERQSRKLLNSRRRKHAARA"
     gene            3071..>3290
                     /locus_tag="VEM33_05275"
     CDS             3071..>3290
                     /locus_tag="VEM33_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYS51267.1"
                     /translation="MIEVPGLRFALGEDIDMLRATVRGFAQAEIAPRAAEIDRSNQFP
                     PDLWKKMGALGLLGVTVEDTYGGTMMGYA"
BASE COUNT          584 a         1043 c         1075 g          588 t
ORIGIN      
        1 atgggcacgc cggcgagcgc gatgccccgc ggctccttca gccggttcgc gaggtacatg
       61 ttcctcaggt agaagctgtg catcttcgcc ggcatgcgcg tggaatccga attccagtag
      121 aggagatcga acgggaaggg atccttcccg agcaggtagt tgttgacgac gaacgaccac
      181 accaggtcgt tcgcgcgcag cgtgttgaag gttgccgcca tctctgagcc ttcgaggtag
      241 ccgcgctcct gcatgcgccg ttccaggttc tcgacctgcg cttcgtcgat gaacaccccg
      301 agctcgcccg ggatggagaa atcgagcagc gacgcgaaga acgtcgccga ggccgcgcgt
      361 cggtcgttct tttgggccat ccacgcgagc gtggcgccga ggagggttcc tccgaggcag
      421 taaccgatga tgttgacctc gttctcgccg gtcgcctggc cgatcgcatc gagcgccgca
      481 agcggccctt cgaacatgta attctcgaac gtcttgccgg catagcgccc gtccgggttg
      541 acccaggaga tcacgaacac cgtgtggccc tgctcggcgg cccaacggac gaaggaattc
      601 ttctcgcgca gatcgaggat gtaatacttg atgatccacg gcgggatcac gagcagcggc
      661 cgtttgaaga ccgcttccgt cctcggggca tattggatga gctgcatgag ctcgctctgg
      721 aaaacgacct tgccgggggt cgtgccgata ttcacgccga gcttgaatgc tttctcgtcg
      781 gccatgcgga ttccgccgcg ctcgatgtcg gcgagcaggt tgttcaggcc cttgagcagg
      841 ttctgaccgc cggagtgcag cgtctcgcgc agcacctgcg ggttggtgag agcgaagttc
      901 gagggagaca gcgcgtcgat gtactggcgc gtgtagaaat tcaccttctt ccgcgtctct
      961 tcgggtaaac cctcgacgga ggaaaccgtg tcgtgaagat gtcgcgcggc gatcaggtag
     1021 gactgcttga tatagtcgaa gaggaactgg tcctgccagc cggcgtcgcg aaaacggttg
     1081 tcccccttgg ctgcctcggc gaccgccggt acttcctttc cgagcatgcg catcaggccc
     1141 gactgccaga gcgcgacgtg gtcccagaac atcttcgtct gcatctccgc gagcttccag
     1201 ggatcggcca gcatgcgtgc agcaagatcc atgaatgcgc gggtgactcc gaattcgtcg
     1261 ctgtaggcga cgccttggcc cccggcctga cgctgcagaa actcccccat gatgcggccg
     1321 gagcgctccg ccacctcggc gaacactctc gcgctctcgg cgggatcggg tgaggactgc
     1381 ggcgcgggca tggcgctagt tttgaacgta acgaaccacg ccgtcggcgc cgaccgaatg
     1441 cttcaagcgc ccgtcgtagt ggagcaaatg caggtgcgcc atggcctcgc ccatggcgaa
     1501 gaagagttga tgcgtgtcga gcttgcggcg aaacagcgta tcgagcacgt ccgccgcggc
     1561 cctgggcgtc tcgcaggctt catgcagctc ggcaaggcgc aggcggtgat gctcctttag
     1621 ctgcgcgatt cgctcgcgca ggcccctgaa cggcaggccg tgcgagggca gcgtgagggt
     1681 ggtcgccggc aatcctgcgt agcgggatat cgagtcgagg aaaagtgcga gcgggtttcc
     1741 ctccggctcg ttcgcccaaa cgctgacgtt ggtcgaaatc ctcgggagca ccatgtcgcc
     1801 ggagatgagg acgccgagct cctcgcagta gagcgctgcg tgttcgggcg catggccgta
     1861 tcccatgacc accttccaat cgcggccgcc gatgcgatgc gcctgccctt ccatcaagcg
     1921 ccggtatgcg gagggaaagc cgggcacccg gctgcgatag ttggtgcggc gctgcttcat
     1981 cagctcgagc ttttctccag atagtccgtt gcgcacgaac atcgccacga cgttgtcgaa
     2041 accgaaaccc gcgccctctt cgcgcatcgc atgcgcggcg aaatactcgg cctgcggcat
     2101 ccacaattcg gcgccggtgc gctcggtcag ccacgaggcg agacccgcgt ggtcgggatg
     2161 atggtgcgtc acgacgaggc gtccgaccgg gcgccctgcg atgcgtccgg cgaagatgcg
     2221 ctcccacagc gcacgcgtgg cgtcatcgcc gatgccgcag tcgatgaatg tccagccctc
     2281 cccgtcctcg agcagccaga ggttgatgtg gtcgagcgca aacggcagcg gcatgcgcag
     2341 ccaatgcacg ccgggcgcga cctgcagggt ttcgccgggc acaggcggcg cggcgtgcgg
     2401 atattccagg atggattcgg cggtcacgcg ctttggatgg cgcgaggagg tcggagtaga
     2461 atccggagct tcccgcggcg aaaggtgcga attctcgcag atcatggcgc cgcccgccac
     2521 aaaccggacg aaagcgctcc gcattctgcc gatgaacgag cccgccgaaa cctacagcat
     2581 cgccgacctc gcgcgcgagt tcgacgtgac tcatcgcgcc atccgcttct acgaggacga
     2641 aggactgctc agccctgcac gcgacggaac gcggcgcgtg tactccaagc gcgactgggt
     2701 gcgcctgaag ctgatcctgc gcgggaagcg gctcggcttc tccctcgccg aggtgcacga
     2761 gatgctggag ctctacgatt ccgcgccgga cgaggggcct cagctggaga aattcgtcgc
     2821 cgcgctcgcc gcacgccgcg agcagctcga gcaccagcgc gaagagatcg aggaggtgct
     2881 ctccgagatc cgagcgttcg agcgccagtc gcgcaagctc ttgaactccc ggagacgcaa
     2941 gcatgcggcg cgggcttgac gcagttgacg tttacgtaaa cgtaaagtac agtctttacc
     3001 ccaccctgtt taccccactc tggaggcttc tcgcgtggac cgagcccgga atctctccgg
     3061 cgggactccg atgatcgagg tgccgggcct gcgctttgcc ctcggcgagg acatcgacat
     3121 gctgcgcgcc acggtgcgcg gcttcgctca agccgagatc gccccgcgcg ccgccgagat
     3181 cgaccgctcc aaccagtttc ccccggattt gtggaagaag atgggcgcgc tcgggcttct
     3241 cggtgtcacc gtggaagaca cctatggcgg gaccatgatg ggttatgcag
//
LOCUS       DASPZD010000273         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1434419, whole genome shotgun sequence.
ACCESSION   DASPZD010000273 DASPZD010000000
VERSION     DASPZD010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434419"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VEM33_05280"
     CDS             complement(<1..181)
                     /locus_tag="VEM33_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYS51268.1"
                     /translation="MTSVIVDSGFLIALFDDTDPLHARCRAFLRDYRGRFLTTEAVLA
                     EAMALISTRQQFRCLE"
     gene            complement(162..410)
                     /locus_tag="VEM33_05285"
     CDS             complement(162..410)
                     /locus_tag="VEM33_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYS51269.1"
                     /translation="MLSVRLDPALAAALERHCAQTGATRSRVVQQSMAQYLVAQSGPT
                     LGDLAEALLPAPAKRRARAARPPRQQRFRDYVRDKRHR"
     gene            complement(485..1261)
                     /locus_tag="VEM33_05290"
     CDS             complement(485..1261)
                     /locus_tag="VEM33_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYS51270.1"
                     /translation="MTILRTLAPFLAAAFALAASPVRAEIKNQWVEYSQGGTKLKGYL
                     TYDDKVTGRRPAVLMIHAREGMTPKTQSLAEIWAKLGYVTFAADMFGYGEGVLPKDVP
                     EMQAQIAIYDKDRSLMRARTQAAFDVLVKNAMVDPSRIALIGYCFGGAVGIEFASTGA
                     SLVANVAIHGSFRDRAPGWAANAKGMFLILHGAEDEGYPLTTVNRLIEELRSAKLPFQ
                     YEVYSGTGHGFSTPKNKAEARANEQSIGTTARTLKELFGI"
     gene            complement(1258..>2561)
                     /locus_tag="VEM33_05295"
     CDS             complement(1258..>2561)
                     /locus_tag="VEM33_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS51271.1"
                     /translation="PLSPANLAANRLAHESASSFSKKTNLTRRRFLVSSCGAASTLLA
                     FNAANAAAGRSGGFFDLPKDAALDQRLARAQVGPAKDEFIFDVQGHFIDTPKGNAKSS
                     EVFLKDVFMDSDTDVMVLSFVPSRRDAEPVTIQAADAVRRVVEKLEGTHRLLLHGRVN
                     PNQPGDLEGMDELKEKWGVSAWKTYTQWGPEGKGFFLSDDIGIRFIEKARKLGVKNIA
                     VHKGLPFGNRSYEHSQCSDIGVVAERFPDVNFLVYHSGLVTGVAEQAYTPGRDGIDTL
                     VASLQKNNVSPGSNVYAELGSTWRFLMRDPEQAAHAIGKLVKHCGPDNVLWGTDSIWY
                     GSPQDQIQAFRAFQIAPVLREKHGYAEITPALRAKIFGRNAAKVYGLSAADIKKYSAS
                     DRIARERNAYLENPEPHFRTYGPKTRREFLRYLRLSAGRLS"
BASE COUNT          393 a          792 c          915 g          461 t
ORIGIN      
        1 attcaaggca gcggaactgc tggcgcgtgg aaattagcgc catcgcctcg gcgagcaccg
       61 cttccgtggt gaggaaccgt ccccggtaat cgcgcaggaa ggcgcggcag cgcgcgtgca
      121 gggggtcggt gtcgtcgaac agggcgatca ggaagcccga atcaacgatg acgcttgtca
      181 cgcacgtagt cccggaagcg ctgctggcgc ggcggacgtg ccgcgcgggc gcggcgtttc
      241 gcgggagccg gcagcagcgc ctcggcaagg tcgccgagcg tcgggccgga ctgcgcgacg
      301 aggtactgcg ccatgctctg ctgcacgacg cggctgcgcg tggcgccggt ctgcgcgcaa
      361 tgacgctcga gcgccgcggc gagtgccggg tcgaggcgga cggaaagcat cgtgctggcc
      421 tttgtcatac gtcggctgat agtgtattac aaagacgccg tccgggccat ggcgccctgc
      481 gatctcagat gccgaacagc tccttgagcg ttcgcgcggt cgtgccgatc gactgctcat
      541 tggcgcgcgc ctcggccttg ttcttcgggg tggaaaagcc gtggccggtg ccgctgtaga
      601 cctcgtactg gaaggggagc ttcgcgctgc gcagctcctc gatcaggcgg ttgacggtcg
      661 tgagcggata gccctcgtcc tcggcgccgt gcaggatcag gaacatgccc ttcgcgttcg
      721 ccgcccatcc cggcgcgcgg tcgcggaacg agccgtgtat cgcgacattc gcaacgagcg
      781 acgcgccggt cgacgcgaat tcgatgccta ccgcgccgcc gaagcagtag ccgatgagcg
      841 cgatcctgga ggggtcgacc atggcgttct tgacgaggac atcgaacgcc gcctgggtgc
      901 gcgcgcgcat cagcgagcgg tccttgtcgt agatcgcgat ctgcgcctgc atttccggaa
      961 catccttcgg cagcacgccc tcgccgtagc cgaacatgtc ggccgcgaac gtcacgtagc
     1021 cgagcttggc ccagatctcc gcgagcgact gggtcttcgg cgtcatgccc tcgcgcgcgt
     1081 ggatcatcag cacggcaggc cgccggccgg tcaccttgtc gtcgtaggtc aagtagcctt
     1141 tgagcttggt gccgccctgg ctgtattcga cccattgatt ctttatctcg gcccgtacgg
     1201 ggctcgcggc gagcgcgaag gcggcggcga gaaagggtgc aagcgtccgg agtatcgtca
     1261 tgagagcctc cctgcgctca accgcaggta ccggagaaac tcccgccgcg tctttggccc
     1321 gtaggtcctg aagtgaggct ccggattctc caggtaggcg ttgcgctcgc gggcgatgcg
     1381 gtcgctcgct gagtatttct tgatgtcggc ggcgctcaag ccgtagacct tggcggcgtt
     1441 gcggccgaag atcttggcgc ggagcgcggg ggtgatttcg gcgtagccgt gcttctcgcg
     1501 taaaaccgga gcgatctgaa aggcgcggaa ggcctggatc tggtcctggg gcgagccgta
     1561 ccagatggag tcggtgcccc agaggacgtt gtcggggccg cagtgcttga cgagcttgcc
     1621 gatcgcatgc gccgcctgct cggggtcgcg catcaggaag cgccaggtgc tgccgagctc
     1681 ggcgtacacg ttcgagcccg gcgatacgtt gtttttctgc agcgaggcga ccagggtgtc
     1741 gatgccgtcg cggccgggcg tgtaggcctg ctccgcgact ccggtgacga ggccggagtg
     1801 gtagacgagg aagttgacgt cgggaaaacg ctcggcgacg acgccgatgt cgctgcactg
     1861 gctgtgctcg taggagcggt tgccgaaggg caggcccttg tgcacggcga tgttcttcac
     1921 gccgagcttg cgcgccttct cgatgaagcg gatgccgata tcgtcgctga ggaagaagcc
     1981 tttgccctcc gggccccact gggtgtaggt cttccacgcg gagacgcccc atttctcctt
     2041 cagctcgtcc atgccttcca ggtctccggg ctggttcggg ttgacgcggc cgtgcagcag
     2101 cagccggtgc gtgccctcga gcttctcgac gacgcggcgc accgcgtcgg cggcctggat
     2161 ggtgacgggc tcggcgtcgc gccgcgacgg gacgaaggag agcaccatga cgtcggtgtc
     2221 gctgtccatg aagacgtcct tcaagaacac ctcggagctc ttcgcatttc ctttcggggt
     2281 gtcgataaag tggccctgca cgtcgaagat gaactcgtcc ttcgccggcc cgacctgcgc
     2341 gcgcgcgagc cgctggtcga gcgcggcgtc cttcggcaga tcgaagaagc cgccgctgcg
     2401 gccggccgcg gcgttcgccg cgttgaaggc gagcagcgtg ctggcggctc cgcaggagga
     2461 aaccaggaac ctgcggcggg tgagatttgt tttcttcgag aaggaggaag ccgattcatg
     2521 cgcgagccgg ttcgccgcga ggttggcggg cgagagcggc a
//
LOCUS       DASPZD010000274         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_415290, whole genome shotgun sequence.
ACCESSION   DASPZD010000274 DASPZD010000000
VERSION     DASPZD010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415290"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="ruvX"
                     /locus_tag="VEM33_05300"
     CDS             <1..346
                     /gene="ruvX"
                     /locus_tag="VEM33_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYS51272.1"
                     /translation="TTLTRRAGKRFPMAQLLDHITRNQVVGIIVGLPLDEAGAEGDAA
                     HAARSLADDIKRRVGEMEVSMWDERMTTARALAAVREMGGSTRGRKEDVDAMAAALLL
                     QHYLDARKGSAL"
     gene            343..1317
                     /gene="mltG"
                     /locus_tag="VEM33_05305"
     CDS             343..1317
                     /gene="mltG"
                     /locus_tag="VEM33_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYS51273.1"
                     /translation="MKRAALLLCLAVACTKPDPDDYQSVADRIMVIIPRGSTYPQALD
                     SLESRGVIKSRDWFSLYARMRGLPSNLKSGIYTFEKDESWSSVVSTLKSGRGLEVRFT
                     VTEGMMGFEVAERARSWLGVSRDSFMVAMRDTSLERELGIQRSPAGVDGYMYPTTYVV
                     PMRMRARDLVKLMTHEFIARWQPEWDVRLNELHMTRHEIVTLASIIEAEVRYRPDRPY
                     VSAVYHNRLKRGMALQADPTIIYAHGRRMRRVWEKQLQIRSPYNTYLNPGLPPGPITQ
                     PSDSSIVAALYPAPIGYLYFVAQPDGKHIFSATYNDHLAAIRRVRNRR"
     gene            complement(1350..1970)
                     /locus_tag="VEM33_05310"
     CDS             complement(1350..1970)
                     /locus_tag="VEM33_05310"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYS51274.1"
                     /translation="MALADQLRLMVITDPVLLKGRDAVAVLRQAVAGGATMVKVRWKD
                     GMPAEILELTRALVAALPVPVLVNDRVDIALAAGAAGAHLGWDDPPLEVLRPTLPAGF
                     LLGLSVGSPDEAARAPATADYWSVGPCFATPTKADAGAPLGPEGFAVLARLAPEGVPV
                     IGIGGITASNAGSITEAGATGVAVIGAVLTARDPESATQELSRALQ"
     gene            1969..2331
                     /gene="aroH"
                     /locus_tag="VEM33_05315"
     CDS             1969..2331
                     /gene="aroH"
                     /locus_tag="VEM33_05315"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HYS51275.1"
                     /translation="MTKILRGLRGATTAAANTSDAILEATEELLSALQEANGFAPEDV
                     ESAIFTSSPDLTAEYPARAARGLGWNDVPLLGTAEVAVPNGLKRCIRVLLHFYTAKPQ
                     QALKHIYLREAAKLRPDR"
     gene            2328..>2886
                     /locus_tag="VEM33_05320"
     CDS             2328..>2886
                     /locus_tag="VEM33_05320"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51276.1"
                     /translation="MTVKVTARGAERWKQGHPWIYRSDVADEPDKKPGIVPVTDRKGK
                     FLGQALYSPSSEIRLRLLTRGTEAIDAQWWTARIAAAASRRNGIQASAWRVVHAEGDG
                     LPSLIIDKYGPWIVAQLLSAGLETARADVLRGIAAALAPEGILLRNDAGIRRHEGLPA
                     EVLLAFGQVPEVVEVVEDDLKYLAAP"
BASE COUNT          524 a          948 c          961 g          453 t
ORIGIN      
        1 caccacgctc acccgccgcg ccgggaagcg attccccatg gcgcagctgc tcgaccacat
       61 cacgcgaaat caggtcgtcg gtattatcgt cggtctgccg ttagatgagg cgggggccga
      121 aggtgatgcc gctcacgccg cgcggagcct cgccgatgat atcaagaggc gcgtgggaga
      181 gatggaggtg agcatgtggg acgagcggat gacgacggcg cgggccctgg ccgcggtccg
      241 tgagatgggt ggctcgacgc gtggccggaa ggaagacgtc gacgcgatgg ctgcagcgtt
      301 gctgctgcag cactatctgg acgcacgcaa gggatcggcg ctgtgaagcg tgcggcgctg
      361 ttgttgtgcc tcgctgttgc ctgcaccaaa ccggatcccg acgactacca gtcggtcgcc
      421 gaccggatca tggtcatcat tccgcgcggg tccacctatc cccaggcgct cgattccctc
      481 gagtcccgcg gcgtgatcaa gagccgcgat tggttcagcc tctacgcgag aatgcggggg
      541 ctgccgtcca acctgaagag cggcatctac accttcgaga aggacgagag ttggagcagc
      601 gtggtcagca cgctgaagag tggccgcggg ctcgaagtgc gcttcaccgt caccgaaggc
      661 atgatgggat ttgaggtcgc cgagcgggcg cgctcctggc tgggcgtatc gcgcgactcc
      721 ttcatggtcg ccatgcgcga cacgtcattg gagcgcgaac tcggcattca gcgcagcccc
      781 gcgggcgtcg acggctacat gtatcccacg acgtacgtcg tgccgatgcg catgcgcgca
      841 cgcgacctcg tgaagctcat gacccatgaa ttcatcgcgc gctggcagcc cgagtgggat
      901 gtacggctca atgagctgca catgacgcga cacgaaatcg tcacgctcgc gtccatcatc
      961 gaagccgaag tgcgctaccg gcccgaccgg ccctacgtct cggccgtcta tcacaaccgg
     1021 ctcaagcgcg ggatggcgtt gcaggccgat ccgacgatca tctacgccca cggccgccgc
     1081 atgcggcggg tttgggagaa gcagttacag atccggtcgc cgtacaacac gtacctgaat
     1141 cccggactgc cgccgggccc catcacgcag cccagcgact cgagcatcgt cgcggcgctc
     1201 taccctgccc cgatcgggta tctctacttt gtcgcgcagc cggacggcaa gcacatcttt
     1261 tccgctacgt acaacgacca tttggcggcg attcggcggg tgaggaaccg ccggtagggg
     1321 gcggtggggg cggtaggagg cggtaggggt tactgaagtg ccctgctcag ctcctgcgtc
     1381 gcagattccg gatcacgggc agtcagaacc gctccaataa cagcgacgcc agtcgctcca
     1441 gcctcagtga tcgaacctgc attcgaggcc gtaatgcccc cgatcccaat aacgggcacg
     1501 ccttcaggcg ccaggcgcgc aagcactgca aagccttcgg ggcccagcgg tgcaccggca
     1561 tcggctttgg tgggcgtggc aaagcagggt cccacgctcc agtagtcggc ggtggccggg
     1621 gcacgcgccg cctcatccgg cgaccccacg gagaggccga gcagaaagcc ggccgggaga
     1681 gtggggcgca gaacctccag cgggggatca tcccagccga ggtgcgcgcc ggccgcgcct
     1741 gcggcgagtg cgatatccac gcggtcattg accaggacgg gaaccggcag ggccgccacc
     1801 agcgcgcggg tcaactccag gatttccgcc ggcatgccat ctttccaccg caccttcacc
     1861 atcgtcgcgc caccggcgac cgcctggcgc aggacggcca ccgcatcccg ccccttgagg
     1921 aggacgggat cggtgatcac catcaagcgc aactgatcgg caagtgccat gaccaagatc
     1981 ctgcgcggcc tgcgtggagc gaccacggcg gccgccaata cgagtgatgc gattctcgag
     2041 gcgacggagg agttgctctc cgcgcttcag gaagcgaacg ggttcgcgcc ggaagacgtg
     2101 gagagcgcga tcttcacgtc gtcccccgac ctcaccgccg agtatcccgc tcgcgcggcc
     2161 cgcgggctcg gctggaacga tgtgccgctc ctcgggaccg cggaagtcgc cgtgcccaac
     2221 ggcttgaaac gctgcatccg cgtgttgctg catttctaca cggccaagcc gcagcaggcg
     2281 ctgaaacata tctacctgcg ggaagcggcg aagctgcgtc cggaccgatg acggtcaagg
     2341 tcactgctcg cggggccgaa cggtggaagc aaggtcatcc gtggatctat cgcagcgacg
     2401 ttgctgacga gccggataag aaaccaggca tcgttcccgt cacagatcgc aaaggcaagt
     2461 tcctcggcca ggcgctctac tcaccgagct ccgagatccg gctccgcctc ctcacgcgcg
     2521 gcaccgaagc catcgacgca cagtggtgga ccgcgcgcat cgcggcagcc gcaagtcgcc
     2581 gcaacggaat ccaggcaagt gcgtggcgcg tcgtgcacgc cgaaggcgac ggcctcccct
     2641 cactcatcat cgacaagtac ggaccgtgga tcgtcgcgca gctgctctcg gcggggctgg
     2701 agaccgcgcg agcggacgtg ctcaggggaa tagcggcggc gctcgctccc gagggcatcc
     2761 tgctgcgcaa cgacgctggg atacggcgcc acgagggcct gcctgccgag gttctgttgg
     2821 ccttcggcca ggtgcccgag gttgtggagg ttgtcgagga cgatttgaag tacctggcgg
     2881 cgccgt
//
LOCUS       DASPZD010000275         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1198621, whole genome shotgun sequence.
ACCESSION   DASPZD010000275 DASPZD010000000
VERSION     DASPZD010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198621"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VEM33_05325"
     CDS             complement(<1..925)
                     /locus_tag="VEM33_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HYS51277.1"
                     /translation="MKKAIALLLILAVAGFVLFRDEFPLPDDSSSTARDPGVRGGAAG
                     AGAPIAGLTKTELAFFSAGTDEFAEEEEASDGLGPTMNLGSCGGCHLQPALGGSSPPV
                     NPQVEFAKAEGATNSVPSFIAPKGPVREARFVKNADGTPDGGVHDLFTIAGRADAPGC
                     ALAQPDFERELARRNVIFRIPTPVFGAGLIEQIPDKAILANQVNDAPRKKELRIRGRP
                     NIVLAGHAISGQANNNGNDGTVARFGWKAQNKSLLLFSGEAYNVEMGITNELFQTERD
                     ETPSCQFAVVPNTVTDTAGETAVDAVSPIEKF"
     gene            1050..1436
                     /locus_tag="VEM33_05330"
     CDS             1050..1436
                     /locus_tag="VEM33_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYS51278.1"
                     /translation="MVRNLLIVWLINALALLALPYLVPSVQVDSITTALVAAALLGLV
                     NTLIRPLIVLLTLPATVLTLGLFIFVINGLLFWFVASFVKGFSVEGFWSAFFGAIVYA
                     LVSWAASALVFGPPRIVVVRHDEHRP"
     gene            1568..2467
                     /locus_tag="VEM33_05335"
     CDS             1568..2467
                     /locus_tag="VEM33_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYS51279.1"
                     /translation="MDTTSPRNSENPSSLYDPAVALDFFQSAGKPQKIAKGTTIFSEN
                     RKGIPLLLMPNRIYLLVEGEVGVFAKDEPIAIIQAGEIFGEMASMGQMPRSASAVAKS
                     DCRVVGLDDKQFRAALGRNPGFALMLMSVMASRLRDTIASLGASPSLADAGWREASAL
                     DGQLLADLAQMVGPAARFRYEAGRIIMREGQLGVALFAVLEGRVAIRIGDSVVEKVGP
                     GGLFGEMSLVDRRPRLATAVAETDCMLLAMSRHMFLHLVKRSPKFGSALLKAVGERAA
                     FMASRRGERSSPSEVPAERLSAA"
BASE COUNT          373 a          851 c          832 g          487 t
ORIGIN      
        1 cgaacttttc gatcggactg acggcgtcga cagcggtctc gcccgcggtg tcggtgacgg
       61 tgttgggaac gacagcgaac tggcagctcg gggtttcatc gcgctcggtc tgaaacagct
      121 cgttggtgat ccccatctcg acgttgtacg cctcgccgga gaacagcagc agcgatttgt
      181 tctgggcctt ccatccgaag cgcgcgacgg tgccgtcgtt gccgttgttg ttcgcctgac
      241 cggagatcgc gtggccggcg agaacgatat tgggccggcc gcggatcctg agctccttct
      301 tcctgggcgc gtcattgacc tggttcgcga ggatcgcctt gtccgggatc tgttcgatca
      361 ggcctgcgcc gaacaccggg gtgggaatgc ggaagatcac gttgcggcgc gcgagctcgc
      421 gctcgaagtc cggctgcgcg agcgcgcatc cgggcgcatc cgcgcggccg gcgatcgtga
      481 acagatcgtg gacgccgccg tccggcgttc cgtcggcgtt cttcacgaac ctcgcctcgc
      541 gcaccggccc cttcggcgcg atgaacgacg gcacgctgtt ggtcgcgccc tccgctttgg
      601 cgaactcgac ctggggattc acgggcgggc tcgatccacc cagtgccggc tgcagatggc
      661 agccgccgca gctcccgagg ttcatcgtcg gtccgaggcc gtcgctcgcc tcttcttctt
      721 ctgcgaactc gtccgtgccg gcgctgaaga acgcgagctc ggtcttggtc agccccgcga
      781 tgggagcgcc cgcgcccgcg gctccgccgc gcaccccggg atcccgggcc gtcgaggacg
      841 aatcgtccgg gagcgggaat tcgtcgcgga agaggacgaa accggcgacc gcgaggatca
      901 ggagcagggc tatggctttt ttcatggtta ccccttcttt gccaaaaaca tggactcccc
      961 ttttgcgcat tcttcgccag gcggagcttt tgtgtcaacg tgcaatcgat gacaatgtca
     1021 tggctgcttg cgctatgctt ggcgttccga tggtgcgcaa cctcctcatc gtctggctga
     1081 tcaacgcgct cgcgctgctc gccctcccct acctcgtgcc ctcggtgcag gtggacagca
     1141 tcaccaccgc gctcgtcgcg gcggccctgc tcgggctcgt caacactttg atccggccgc
     1201 tcatcgtgct gctcacgctg cccgccaccg tcctcacgct gggattgttc atcttcgtga
     1261 tcaacggcct gctcttctgg ttcgtcgcgt ccttcgtcaa gggattctcg gtcgaggggt
     1321 tctggtcggc gttcttcggc gcgatcgtct acgcgctcgt ctcctgggcg gcgagcgccc
     1381 tcgtgttcgg cccgccgcgc atcgtcgtgg tgcggcacga cgagcaccgc ccctagcgca
     1441 aagccgcgaa ctccccgcga cgcggagcga agtatgaaat atgtccgctc cgcggcttca
     1501 tttcctcctc ccggcctcgg gccgcgggca taatcgcgcg ctggtccgat ccccgggagg
     1561 agagagtttg gatacgacga gtccgcgcaa ttccgaaaac ccttcttcgc tctacgatcc
     1621 cgccgtcgcc ctggatttct tccagtccgc gggcaagccg cagaaaatcg caaagggcac
     1681 gaccatcttc tccgagaacc gcaagggcat tccgctgttg cttatgccca accgcattta
     1741 cctcctggtg gaaggggaag tcggcgtttt cgcgaaagac gaacccatcg ccatcatcca
     1801 ggccggcgag attttcggcg agatggcctc gatggggcag atgccgcgca gcgcgtccgc
     1861 ggtggcgaag agcgactgcc gggtggtcgg cctggacgac aagcagtttc gcgcggcgct
     1921 cggcaggaat ccggggttcg cgctcatgct gatgagcgtc atggcaagcc gcctgcgcga
     1981 tacgatcgcg agcctgggcg cctccccttc gctggcggac gccgggtgga gggaagcttc
     2041 agcgctcgac gggcagctgc tcgccgatct cgcgcagatg gtcggccccg cggcgcgttt
     2101 ccgctacgag gccgggagga tcatcatgag ggaggggcag ctgggcgtgg cgctgttcgc
     2161 ggtgctcgaa ggccgcgtcg cgatccgaat cggcgattcg gtcgtcgaga aagtcggccc
     2221 gggcggcctg ttcggcgaaa tgtcgctcgt cgaccgcagg ccgcgcctcg ccaccgccgt
     2281 ggccgagacc gactgcatgc tgctcgcgat gagccgccac atgttcctgc acctggtgaa
     2341 acgcagcccg aaattcggct ccgcgctgct gaaggccgtg ggcgagcgcg cggccttcat
     2401 ggcctcgcgg cgcggcgagc gaagctcgcc ttccgaggtc cctgcagaac ggctctccgc
     2461 cgcctgaccc ggcgcgatca gcgccggtac atgtacccca ggtataccgc gccgaactgc
     2521 gaattgtcgg gcttggtgct cga
//
LOCUS       DASPZD010000276         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1785709, whole genome shotgun sequence.
ACCESSION   DASPZD010000276 DASPZD010000000
VERSION     DASPZD010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785709"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..307
                     /locus_tag="VEM33_05340"
     CDS             71..307
                     /locus_tag="VEM33_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HYS51280.1"
                     /translation="MTSPQRAKKAVNVSISADVLEAARSHEINLSATLESALEDQLRQ
                     RRRKEWLVTNGDSIEAYNRDVEKRGTFGDSLRSF"
     gene            307..624
                     /locus_tag="VEM33_05345"
     CDS             307..624
                     /locus_tag="VEM33_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HYS51281.1"
                     /translation="MPQFSVYRNKNAATRARFPFLLDIQSDLLDPLATRVVVPLATAG
                     PARSRSMQTLTPAIRVEGKNYLMITPQLAGVAARELGPVVADASAGHPAIIAALDFLI
                     SGI"
     gene            1047..2345
                     /locus_tag="VEM33_05350"
     CDS             1047..2345
                     /locus_tag="VEM33_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS51282.1"
                     /translation="MSPVQSQASVEASGETPARILVVEDERIVAHDLASALRELGYAV
                     PATVATGEEAVERARELQPDVVLMDIRLPGAIDGIRAASSIRQEHDIAVIYLTAHSDD
                     ETLRRAMQTEPVGYLVKPFSPPQLRCAIEIALHRREINARLHERQRWLATRMRCADDA
                     EAAEEAGPKVSYLNLVAEALAGWRLGESEREKRMAEHIADLEAANRELENFTCSVARD
                     LRGPLRGIDGLSQSLIEDHAANLGPQGLRTLRQVREAAGRMRQVIDNLLVLSIDDLLL
                     LSQVSKTEARRDTIDLSRIAMGVVAQLRESTPQREVKVVIERDVRADADASLLRIVLE
                     NLLGNAWKFTGEVEHARIEFGAFDRDGSKVFFVRDNGAGFDMKEAEKLFVAFQRLDSA
                     GQFEGNGLGLAIAQRAINRHGGRIWAEGAPARGATFYFAL"
     gene            2372..>3359
                     /locus_tag="VEM33_05355"
     CDS             2372..>3359
                     /locus_tag="VEM33_05355"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HYS51283.1"
                     /translation="MFKTIAYTAGVALAYYVTGRLALILAIPPGYATAVWPAAGVALV
                     AILLLGYRVWPGILLGHFFVNILTGYDTSSSGAALKSLALPLAIACGGTLQAVCGAAL
                     IRRYVGSTIVLQTEREIAKFLALGGPVSCLISATVGLVCLYAWGLVSGPDIPFSWFTW
                     WVGDTIGVTVMAPLLLAWLVLPRDRWRAGRAALTWSTAVALSLAVVLYVYISGREQQG
                     IRAEFERAADTLGREFVNDVEGHLEAVTALHDLYVSAGPLDRASFAAFAGPLQMRHPG
                     VQALEWAPVVPQAQRASLEEAVRREGYPAFAITDRGPDGRMVPAPTRAEYVPV"
BASE COUNT          574 a         1061 c         1099 g          625 t
ORIGIN      
        1 gccagcgagg tattggcgcg cacgtacaat gcgcatatcc gatgcgcatc acacgcgaat
       61 cccccaaagc atgacgtccc cacagcgcgc gaagaaggcg gtcaacgtgt cgatctcggc
      121 ggacgtgctg gaggctgccc gcagtcacga gatcaatttg tcggcgacgc tggagtccgc
      181 tctcgaagat cagctgcggc agcggcggcg caaggaatgg ctggtaacga acggtgacag
      241 catcgaggcc tacaaccgcg acgtcgaaaa acgcggcacg ttcggcgact ctctacgcag
      301 cttctgatgc cacagttctc cgtgtaccgg aacaagaatg cggcgacgcg ggcacggttc
      361 cccttcctcc tcgacatcca gtcggatctg ctcgatccac tggcgacacg cgtcgtcgtg
      421 ccgctcgcga ctgcgggccc agcccgttcg cgatcaatgc aaacgcttac gccagcgatc
      481 cgagtagaag ggaagaacta tctgatgatc actccccagc tcgctggcgt tgcagcccgc
      541 gagctcgggc cggtggtggc cgatgcctcg gccggccacc cggcgatcat cgcggcgctt
      601 gattttctga tttccgggat ctgagtacgt tccgacgcca atgcggtctc tgaggcttgc
      661 gcaaaccgcg ccgaaagcgc cagccgggat tcaacgccac agcaggattc ccgtcctgct
      721 ccccctcctg ctcctcctat tcctattcct tatcccaact tcaccaatac tttctaatta
      781 gcgacgaagg cggcgattgc aattcggaag ggtcggcgag tcaggtcctt ttcatttcac
      841 aaatgtcagc cggaacccgg attggacgac aggtaaggcg cttctccggc atcggcgtgt
      901 ggcaggacaa tccggccgtg ccggccatct gatcggcgca gtccgttggc gtcatggaca
      961 ctgggcactt ttcattgaag gaattcgacc gatacgatac agtccaccgg gcttccaatt
     1021 tggtgcggcc actggaggac tgcgcggtga gtccggttca gtctcaggcc tcggttgagg
     1081 cttcgggcga gacgcctgcc cggattttgg tcgtcgagga cgaaagaatc gtggcccacg
     1141 acctcgcgag cgccctgagg gagctcggct acgcggtccc ggcgaccgtc gcgaccggcg
     1201 aagaggcggt tgagcgcgcg cgcgagctgc agccggacgt ggttctgatg gacatacgac
     1261 tgcccggagc gatcgacggc atccgggccg cctcttcgat ccgccaggag cacgatatag
     1321 cggttattta cctgaccgcg cattccgacg acgaaacctt gcgccgggcg atgcagaccg
     1381 agccggtcgg ctacctggtg aagccttttt ccccgccgca gctgcggtgc gcgatcgaga
     1441 tcgccctgca tcggcgcgag atcaacgctc gcctgcacga gcgccagcgc tggctcgcga
     1501 ccaggatgcg ctgcgccgac gatgcggagg ctgcggagga agccgggccg aaggtgagct
     1561 acctgaacct ggttgccgaa gccctggcgg gatggcgtct cggcgagagt gagcgcgaaa
     1621 agcgcatggc cgaacatatc gcggatctgg aggccgcaaa ccgggaactg gagaatttca
     1681 cctgcagcgt cgcccgggac ttgcgcggcc cgctacgggg aatcgatggc ctgagccaat
     1741 cgctgatcga ggaccacgcg gccaacctgg gtccgcaagg gctcaggacg ctcaggcagg
     1801 ttcgcgaggc cgccgggcgg atgagacagg tgatcgacaa tcttttggtg ttgtcgatcg
     1861 acgacctgtt gctgctgtcg caggtgtcga agactgaagc gcgcagagac acgatcgatc
     1921 tgtcccggat cgctatgggc gtcgtggctc aattgcgcga aagcaccccg cagcgtgagg
     1981 tcaaggtcgt catcgaaaga gacgtccggg ccgacgccga cgcgtccttg ctgcggatcg
     2041 tgctcgaaaa tcttctgggc aacgcctgga aattcaccgg ggaggtcgag catgcacgga
     2101 tcgagttcgg cgccttcgac cgagatggct ccaaggtctt cttcgttcgg gacaacggag
     2161 cgggcttcga catgaaggaa gcggagaagc ttttcgtcgc cttccagcgt ctcgattcag
     2221 ccggtcagtt cgagggcaat ggccttggat tggccatcgc tcaacgtgcc atcaaccggc
     2281 acggcggtcg gatctgggcc gaaggggctc cggcccgagg tgcgacgttc tatttcgccc
     2341 tctagttttt cggctgtccg cccatccagg cgtgttcaag accatcgcct acactgcggg
     2401 cgttgcgctt gcctattacg tcaccggtcg cctcgccctg atcctggcca ttccgcccgg
     2461 ctacgcgacg gcggtctggc ccgcagccgg tgtcgcactg gtggcgatcc tgctgttggg
     2521 ctaccgggta tggcccggga tactgctcgg tcacttcttc gtcaacatcc tgacaggcta
     2581 cgatacctcg agctccgggg cggcgctcaa gtccctggct ttgcctctgg ccatcgcctg
     2641 cggcggaacg ctgcaggccg tctgcggcgc cgcgctgata cgacgctatg tcggatcgac
     2701 gatcgttctt caaaccgaga gggagatcgc gaagttcctc gcgctgggcg gccctgtcag
     2761 ttgtctgatc agcgcaaccg tcgggctggt ctgtctgtat gcgtggggcc tggtctccgg
     2821 tccggatatc ccgttcagct ggttcacgtg gtgggtcgga gacacgatcg gcgtgacggt
     2881 catggcgccg ctgctgctcg cctggctggt gctcccgcga gatcgctggc gcgcgggccg
     2941 cgcggcgctg acatggtcca ccgcggtggc gctgtcgctc gccgtcgtgc tgtatgtcta
     3001 tatcagcggt cgggagcaac agggaatccg cgccgagttc gaacgcgcgg cggacacgct
     3061 gggccgggag ttcgtcaacg acgtcgaggg acatctcgaa gcggtcaccg cgcttcacga
     3121 cctttacgtc agcgccggac cgctggaccg cgcgagcttc gccgctttcg ccgggcctct
     3181 tcagatgcgg cacccggggg ttcaggcgct cgaatgggcc ccggttgtgc ctcaggccca
     3241 gcgggccagc ctcgaggagg cggtgcgtcg cgaaggctac ccggcgttcg cgatcaccga
     3301 ccggggcccg gatggccgga tggttccggc gccgacccgc gccgaatacg tgccggtat
//
LOCUS       DASPZD010000277         8463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1605155, whole genome shotgun sequence.
ACCESSION   DASPZD010000277 DASPZD010000000
VERSION     DASPZD010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605155"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VEM33_05360"
     CDS             complement(<1..288)
                     /locus_tag="VEM33_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYS51284.1"
                     /translation="MTFEGKTCLITGAAGNLGRAVAAAFASAGASLILVDLDDKVLRS
                     AFGRDDERKLLVPADLLDAASVAQALAAVRFQRIDVLCNIAGGFRMGKPVHE"
     gene            complement(333..989)
                     /locus_tag="VEM33_05365"
     CDS             complement(333..989)
                     /locus_tag="VEM33_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51285.1"
                     /translation="MAKQRISYVPLEKMDAEMRKEMERCQREGTPRPESSAVRAHVPK
                     CFWAFANSWRDIFRNGVCDHSIKELCRLYVSRSVQCEYCGNQRSIKAASSGAVMEDHV
                     KDLLNFEKSGNYDERQKAALAYAEAITWHLNTDDAFWERLHGHFSEPELVELGCFIAL
                     TMGQQSWLRLLNIDHHQVLAGTSASMAPGFEDAKALAEAKASSEYWAKSAAEKSKSAA
                     "
     gene            complement(1130..2092)
                     /locus_tag="VEM33_05370"
     CDS             complement(1130..2092)
                     /locus_tag="VEM33_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51286.1"
                     /translation="MKKIIGLAVVGLAAAASQASAQGYPAKPVHVIISFTPGSSTDIV
                     GRVVAQKLAEMWGQPVLAENRAGAGGSIGTAFVAKAPADGYTLLVDSNAHTVTPSIYA
                     KLPYDVEKDFVDIVPLAVQPNVLVVNAGSPYKTLMDLVNAAKAKPGAINWGHAGIGSG
                     THLNTEKFVDAAKINVTQVPFKGTPEVIQAMLSQSVDCYWAPISAAIPHIKGGRVRPL
                     AVSTAKRNGQLPDVPTTGEAGVKGADAPLWFALWGPAGIPAGIVNKISADTRKALKDP
                     AVRDRLVGLGNDTMDMSPEEFKIFVQSELREYARVIKAAGIQPQ"
     gene            complement(2114..3046)
                     /locus_tag="VEM33_05375"
     CDS             complement(2114..3046)
                     /locus_tag="VEM33_05375"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYS51287.1"
                     /translation="MSSGKNARIGWIGAGRMGFEMARRLAKGGCDIAVWNRTRAKAEP
                     LTKDGAKIVEGLKDLAGCDIVFCMVSTYDDVKEVIAGPKGLLSGPTKPRMVVECSSIS
                     LEGSAELRKILAARGVELLSAPVSGNAKVIKAGKLTFVVSGPKSAYDAAAPYLDMMGQ
                     GSSYVGEGELSRIVKICHNVMLGVVTQCMAEITVLAQKAGVPRHAFLDFLNKSVMGST
                     FTRYKAPAFVNLDFHVTFTPYLLRKDMDLGLAAGRNFEVPMPLASLTRDLIQSMMGAG
                     MTEEDFSTLLLQQAKASGIELKPEDKAVGDGLSQ"
     gene            complement(3062..3301)
                     /locus_tag="VEM33_05380"
     CDS             complement(3062..3301)
                     /locus_tag="VEM33_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYS51288.1"
                     /translation="MDVIMPQLGETVTEGTVANWYKKVGDTVKADEPLFDVETDKVTT
                     EVPSITAGVVKAILVEAGKTVPVGTVLAVIEPSPK"
     gene            complement(3316..4299)
                     /locus_tag="VEM33_05385"
     CDS             complement(3316..4299)
                     /locus_tag="VEM33_05385"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYS51289.1"
                     /translation="MAQLSYRDAVAAAIAQEMRRDERVVLFGEDTAAAGGVFKTTVGL
                     FEEFGPRRVKDTPISELAILGAAMGAAMTGLRPVAEIMFSDFLACCWDYVANEIAKTR
                     YMSDGQISIPLVIRTANGAGSRFGAQHSQSVENWAMAIPGLKVVAPSTPADAKGLLAA
                     AIRDPDPVLFFEHKSLLANKADVPEGEHVEPLGKARVLRTGADATIIALAAMVPRAEK
                     AAEALAADGIKATVIDVRSLVPLDAKTILAESARTGCVFTVEENPRLCGWGAEIASII
                     QEEAFSSLKGPIIRITTPHVPLPSADQLEDAVVPTVARIVAEVKRALSRAA"
     gene            complement(4287..5309)
                     /locus_tag="VEM33_05390"
     CDS             complement(4287..5309)
                     /locus_tag="VEM33_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYS51290.1"
                     /translation="MSFTREAHPTHLSQEIPQATRLRLFQLQVEIRYCEKRAYDLFLQ
                     NLVKGTSHLALGQEAVAAGFAAAMNPDDYTFCTYRGHHHTLARGAPMLSVLGELMGRQ
                     CGLMKGKGGSMHLTSVAHGVMGSYAIVGAHLPIAAGAAWSAQYRGTKQVAVAFFGDGA
                     TNIGAFHEALNLAAVWKLPVVFCCENNMYMEYTPISAVTAVKHPAADRAASYGLERQV
                     IDGNDADIVYSAARDAFAKARSGGGPSLVECLTYRHSGHSRADPAKYRPKEELEEWLK
                     RDPVTLYRNRLLASGVAEGTLAKIESEAMAKLDQATEAAKASPTPAVETAMTDVWADG
                     GNAWRN"
     gene            complement(5327..6640)
                     /locus_tag="VEM33_05395"
     CDS             complement(5327..6640)
                     /locus_tag="VEM33_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYS51291.1"
                     /translation="MAKPKKTAEPKIIRPDDIDPHHNWTRPLHAPGHTQVDFEERINF
                     RRLHDYRLARVRAALAASGLGALLSFDQHNIRYTTSTVIGEWARDKLTRYSLLTGNGD
                     PYIWDFGSAAKHHRLYAPWLHVDHCKAGLLGLRGAVGSDITLFREAATEIKAILDAEG
                     VGDMPLGLDVVEPPMLFELQKLGIEVRDGQQVMLQAREVKNIDELTLLNMASAMVDGV
                     YQDIAEALKPGVKESEIVALATSRLYQMGSDCVEAINSISGERCSPHPHNFTDRLIRP
                     GDQAFFDVIQSFMGYRTCYYRTFNVGRATAPQRTAYRKAREWMDKAIDLLKPGVASDK
                     IARAFPEAKEIGFSSEMEAFGLNFCHGLGLGLHERPLISRLTSFKEPIELKAGMVFAV
                     ETYCPASDGISAARIEEEVILTPEGAKIISLYPAEELPIANPYRS"
     gene            complement(6710..7669)
                     /locus_tag="VEM33_05400"
     CDS             complement(6710..7669)
                     /locus_tag="VEM33_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51292.1"
                     /translation="MLKRICWTALAGASLASGIAAAQTFPSRTIHLVSGVTPGSASDT
                     TARIVAEKLQASLGQAVIVENKLGVGGLIGAAYVAKADPDGHVISIYTSAFTVAPLLS
                     PGPLDPKELTPVATLGTVPTVLVVSTAKSYKTVADLVAAAKAKAGAVVATTAGIGSST
                     HMNLERFRISAGIEVLHVPMKGAPDALTEVLTGRADFYFALPFQVGPQVKEGKLRALA
                     VGSPKRSALFPDVPTTVEAGYPNSDYNFWIGALVASRTPRDIVLRLNKEFNAAVNSSE
                     VREKFLKLGVDPMSMSLPDFEAMIKKEFESNAQLIKAAGIRAN"
     gene            complement(7692..>8463)
                     /locus_tag="VEM33_05405"
     CDS             complement(7692..>8463)
                     /locus_tag="VEM33_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYS51293.1"
                     /translation="ECYKAALPHVRPPGERVAIPFEGKTLYGVLRKPAGAGRLPILIM
                     APGLDSTKEELQAYAEPFLARGIAILAIDGPGQGEAEYEIPICGDYERAAHAVCDWIE
                     EQPELDAAKIAMWGVSLGGYYAPRATAYEKRIKACIALSGPFEWKEIWSALPVLTREA
                     FRVRSHCATEADALRRAATLSMKEAAAKIACPIFIVTGREDRLVPASHAERLAKGVSG
                     PVELLIVEDGGHNANNRPYRYRSRTADWLAGQLGLPRI"
BASE COUNT         1340 a         2832 c         2705 g         1586 t
ORIGIN      
        1 ctcgtgcacc ggcttcccca tgcggaagcc gcccgcgata ttgcaaagaa cgtcgatccg
       61 ttgaaaccga acggccgcaa gcgcctgcgc aacggacgct gcgtcgagca gatcggcggg
      121 caccaggagc ttgcgctcat cgtcgcgccc aaaggcggac cgcaggacct tgtcgtcgag
      181 atcgacgagg atcagggacg cgcccgccga cgcgaaagcc gcggcaaccg cgcggcccag
      241 attgccggca gcgccggtga tgagacaagt cttgccttcg aaggtcacaa acccgctcca
      301 cgtgcccggg agactggctc cggcccctga agtcaagcgg cggatttgga cttttctgcc
      361 gcggacttcg cccaatattc ggacgaagcc ttcgcctcgg caagcgcctt ggcgtcctcg
      421 aaccccggcg ccatcgacgc tgacgtcccg gcgagcacct ggtggtggtc gatgttgagc
      481 aacctcagcc agctctgctg ccccatcgtg agcgcgatga agcagcccag ttcgaccagc
      541 tcgggctcgc tgaaatgccc gtggaggcgt tcccagaacg catcatcggt gttcaggtgc
      601 caggtaattg cctcggcata cgcaagggcc gccttctggc gctcgtcgta attgccggac
      661 ttctcgaagt tcagcaggtc cttgacgtga tcctccatga cggcgccgct cgaggccgcc
      721 ttgatcgagc gctgattgcc gcagtattcg cattgaacgg agcgcgatac gtagagccgg
      781 cacaactctt tgatggagtg gtcacacacg ccgttcctga agatatcgcg ccaggagttg
      841 gcaaaggccc agaagcattt gggcacatgc gcgcgcaccg cggaactctc cggcctgggc
      901 gtgccctctc gctggcagcg ctccatctcc ttgcgcattt ccgcgtccat cttttccagc
      961 ggaacgtagc tgattctctg ctttgccatg gatgtcctcg ccggccgcat ccggccggtc
     1021 tctcaaattc cggtggttcg aagtctgcat cttcggagcc ggggttgcaa ggtcgacggg
     1081 atccatcggg cgggccgcgc ttaccgggct cgcgcaaacg gctgaggcat cactgcggct
     1141 ggatgccggc cgccttgatg acgcgggcgt attcccggag ttcgctctgc acgaatatct
     1201 tgaattcctc cggcgacatg tccattgtgt cattgcccag gccgacgagc cggtcccgca
     1261 ccgccgggtc cttgagcgcc ttgcgcgtgt cggccgagat cttgttcacg atccccgctg
     1321 ggatgcccgc cgggccccac agtgcgaacc acagcggcgc atcggcgcct ttaacacccg
     1381 cttcgccggt ggtcggtacg tccggcaact ggccgttgcg cttcgccgtg ctcaccgcga
     1441 gcggccgaac cctgccgccc ttgatgtgcg gtatcgccgc cgagatcggc gcccagtagc
     1501 aatccaccga ctggctcaac atcgcctgga tcacttccgg cgtgcccttg aacgggacct
     1561 gggtgacgtt gattttcgcc gcgtcgacga acttctccgt gttgaggtgc gtgccgctgc
     1621 cgatcccggc atggccccag ttgatcgcgc ccggtttcgc cttcgccgcg ttgacgaggt
     1681 ccatcagggt cttgtagggc gaaccggcgt ttaccacgag gacgttcggc tgcaccgcga
     1741 gcggcacgat atcgacgaag tccttctcca catcgtaggg cagcttcgcg tagatcgacg
     1801 gcgtcaccgt gtgcgcgttg gaatcgacga gcagcgtata accgtccgcc ggcgccttgg
     1861 cgacgaatgc cgtgccgatc gagccgccag cgccggcgcg gttttccgcg agcaccggct
     1921 ggccccacat ctccgcaagc ttctgcgcca ccacgcgccc cacgatgtcg gtcgagctcc
     1981 cgggcgtgaa ggaaatgatc acgtgcaccg gcttggcggg gtagccctgc gccgacgctt
     2041 gggacgcggc tgcagccagc cctactacgg caaggccaat tatttttttc atctgaaact
     2101 cctctgtgaa agattattgc gacagcccgt cgccgaccgc cttgtcttcc ggcttcagct
     2161 cgatgcccga ggccttcgcc tgctgcagca gcagggtcga gaagtcctcc tcggtcattc
     2221 cggcgcccat catcgactgg atcagatcgc gagtcaggga cgcgagcggc atcggcactt
     2281 cgaaattccg gccggcggcc aaccccaggt ccatgtcctt gcgcagcaga tatggcgtaa
     2341 acgtgacgtg gaagtcgaga ttgacgaatg ccggcgcttt gtagcgggtg aaggtcgagc
     2401 ccatcacgct cttgttcaag aaatcgagga aggcgtggcg cggcacgccc gccttctgcg
     2461 cgagcaccgt gatctccgcc atgcactgag tcaccacgcc cagcatcacg ttgtgacaga
     2521 tcttgacgat gcgcgaaagt tccccttcgc cgacgtagga cgagccctgc cccatcatgt
     2581 ccaggtaggg cgccgctgcg tcgtacgcgg acttggggcc cgagacgacg aaggtgagct
     2641 tgcccgcctt gatcaccttg gcattgcccg agaccggcgc ggagagcagc tcgacgcctc
     2701 tcgccgcgag gatcttgcgc agttcggcag agccctcgag cgatatcgag gagcactcga
     2761 ccaccatacg cggcttggtg ggaccggaaa gcaatccctt tggccccgcg atcacttcct
     2821 tgacgtcgtc gtaggtcgac accatgcaga acacgatgtc gcagccagcg aggtctttca
     2881 ggccttcgac gatcttcgcg ccgtccttgg tgagaggttc ggccttggcg cgcgtgcggt
     2941 tccacaccgc gatgtcgcac ccccccttgg cgaggcgccg cgccatctcg aaacccatgc
     3001 ggccggcgcc gatccagccg attcttgcgt tcttgccgga gctcaattca gtctccttcg
     3061 ttcatttcgg cgagggctcg atcacggcca gcaccgtccc gaccgggaca gtcttgcccg
     3121 cttcgaccag gatggctttc accactccag ccgtgatcga cggaacttcg gtcgtcacct
     3181 tgtcggtctc gacgtcgaag agcggttcgt ccgctttgac cgtatcgccc actttcttgt
     3241 accagttggc gacggtgcct tcggtcacgg tctcgccgag ctgcggcatg atcacgtcca
     3301 tcgctcttct ccgttctacg cggcgcggct caaagcccgc ttcacttcgg cgacgatgcg
     3361 cgcgaccgtg ggcaccaccg catcctccag ctggtccgcg gaaggcaagg gcacgtgcgg
     3421 cgtggtgatc ctgatgatcg gtcccttgag agagctgaag gcttcctcct gtatgatcga
     3481 ggcgatctcg gcgccccagc cgcacaggcg tggattctcc tcgacggtga acacgcagcc
     3541 ggtgcgcgcc gactcggcga gtatcgtctt ggcgtccagc ggaacgagag agcgcacgtc
     3601 gatcactgtc gccttgattc cgtctgccgc gagcgcttct gcggccttct ccgccctcgg
     3661 gaccatcgcg gcgagcgcga tgatcgtcgc gtcggcgccg gtgcgcagca cccttgcttt
     3721 cccgaggggc tcgacgtgct cgccctcggg cacgtcggcc ttgttggcga gcaggctctt
     3781 gtgctcgaag aacaggaccg ggtcgggatc acgaatcgcg gcggcgagca gtcccttcgc
     3841 gtcggccggc gtggaaggcg ccacgacttt caggcccggt atagccatcg cccagttctc
     3901 cacgctctgc gaatgctgcg cgccgaagcg cgagcccgcg ccgtttgcgg tccggatcac
     3961 gagcgggatg ctgatctgcc catcgctcat gtagcgggtc ttggcgatct cgttggcaac
     4021 atagtcccag cagcaggcga ggaagtccga gaacatgatc tccgccaccg gccgcaatcc
     4081 cgtcatcgcc gcgcccatgg ccgcgccgag gatggcgagc tccgagatcg gggtgtcctt
     4141 cacccgcctc gggccgaact cctcgaagag tccgaccgtg gtcttgaaca cgccgcctgc
     4201 ggcggcggtg tcttcgccga agagcacgac gcgctcgtcg cgcctcattt cctgcgcgat
     4261 cgcggcagcg actgcgtccc ggtagctcag ttgcgccatg cgttgccccc gtcggcccac
     4321 acgtcggtca tcgccgtctc caccgcaggc gtgggcgagg ccttcgccgc ttccgtcgcc
     4381 tgatcgagtt tcgccatcgc ttcggattcg attttcgcga gtgtgccttc cgcgacgccg
     4441 ctcgcgagca agcggttgcg gtagagcgtc accggatcgc gcttgagcca ctcctcgagc
     4501 tcctctttcg gacggtattt cgccgggtcg gcgcgcgagt gcccgctgtg ccggtaggtg
     4561 aggcattcga cgagggaggg gccgccgccc gagcgcgcct tggcgaaggc gtcgcgcgcg
     4621 gcgctgtata cgatatcggc gtcgttgccg tcgatgacct ggcgttccag accgtagctt
     4681 gcggcgcggt ctgcggccgg gtgtttcacc gcggtgactg cggagatcgg cgtgtactcc
     4741 atgtacatgt tgttctcgca gcagaagacg accgggagct tccacaccgc agcgaggttg
     4801 agggcctcgt ggaaagcgcc gatgttggtc gcgccgtcgc cgaagaaggc gaccgcgacc
     4861 tgcttcgttc cgcggtactg cgccgaccac gcggcgccgg cggcgatcgg caggtgcgcg
     4921 ccgacgatag cgtaggagcc catcaccccg tgcgcgacgc tcgtgagatg catcgagccg
     4981 cccttgccct tcatcaggcc gcactgccgc cccatcagct cgcccagcac cgagagcatc
     5041 ggagcacccc gcgcgagcgt gtggtgatgg ccgcgatagg tgcagaaggt gtagtcgtcc
     5101 gggttcatcg ccgcggcgaa gcccgccgcg accgcctcct ggccgagcgc gagatggctc
     5161 gtccccttca ccaggttctg caggaaaaga tcgtaggcgc gcttctcgca gtagcggatc
     5221 tcgacctgca gctggaacag gcgcagacgc gtcgcctggg ggatctcttg gctcagatgc
     5281 gtcggatgtg cctctcgggt gaagctcatg tcgggaactg gctcccttag cttcggtacg
     5341 gatttgcgat cggcagctct tcggccggat agagcgagat gatcttcgcg ccctcgggcg
     5401 tgagaatcac ttcttcctcg atgcgcgcag cggagatgcc gtcgctcgcg gggcagtagg
     5461 tttcgaccgc gaacaccatc cctgccttca gctcgatcgg ctccttgaaa gaagtgagcc
     5521 gcgagatcag cggccgctca tgcaggccga gccccagccc gtggcagaag ttgaggccaa
     5581 aggcctccat ctcgctcgaa aagccgatct ccttcgcctc cgggaaggcg cgcgcgatct
     5641 tgtcgctggc caccccgggc ttcaggaggt cgatcgcctt gtccatccac tcgcgcgcct
     5701 tccggtacgc agtgcgctgc ggagccgtcg cacggcctac attgaacgtc cggtagtaac
     5761 aagtgcgata gcccatgaac gactggatca cgtcgaagaa ggcctggtcg ccggggcgga
     5821 tgaggcggtc ggtgaagttg tgcgggtgcg gcgagcagcg ctcgcctgaa atggagttga
     5881 tcgcctcgac gcagtccgag cccatctggt agagccgcga ggtcgcgagc gcgacgatct
     5941 cggattcctt gacgccgggc ttgagcgcct cggcgatgtc ctggtagacg ccgtcgacca
     6001 tcgccgaggc catattgagg agggtcagct cgtcgatgtt cttcacttcg cgcgcctgga
     6061 gcatcacctg ctggccgtcg cgcacctcga tcccgagctt ctgcagctcg aacagcatcg
     6121 gcggctcgac cacgtccagg ccgagcggca tgtcgcccac gccttcggcg tcgaggatcg
     6181 ccttgatctc cgttgccgcc tctcgaaaca gcgtgatgtc gcttcccacc gccccgcgca
     6241 atcctaggag ccccgccttg cagtggtcga cgtgcagcca gggcgcgtag agccggtggt
     6301 gcttggctgc gctgccgaag tcccagatgt aggggtcgcc gttgccggtg aggagcgagt
     6361 agcgcgtcag cttgtcgcgc gcccattcgc cgatgacggt gctcgtcgta tagcggatgt
     6421 tgtgctgatc gaatgagagc agcgcgccca gtcccgaggc cgcgagcgcg gcgcgcacgc
     6481 gcgcgaggcg gtagtcgtgc agccgcctga agttgatgcg ctcctcgaaa tcgacctggg
     6541 tgtggcccgg cgcgtgcagc gggcgcgtcc agttgtggtg cggatcgatg tcgtccggac
     6601 ggatgatttt cggctcggcg gtttttttcg gcttggccat cgattttctt ccaggacgac
     6661 tgttttgcgg aacgcgaagt atgccacgcg gccgctcaag cggcccgctc taattggccc
     6721 ttatgcccgc cgccttgatc agctgggcgt tcgactcgaa ctccttcttg atcatcgcct
     6781 cgaaatcggg cagcgacatg ctcatgggat cgacgcccag cttgagaaat ttctcgcgca
     6841 cctcggacga gttcaccgcc gcgttgaact ccttgttcag gcgcagaacg atgtcgcgcg
     6901 gggtcctgga ggcgaccagc gcgcctatcc agaagttgta atcggagttc ggatacccgg
     6961 cctcgacggt cgtggggacg tcgggaaata gcgcgctcct cttgggcgag cccaccgcga
     7021 gcgcgcgcag cttcccctcc ttgacctgcg gccccacctg gaacggcagg gcaaaataga
     7081 aatcggcgcg accggtgagc acttcggtga gcgcatccgg cgcgcccttc atcggcacgt
     7141 gcagcacctc gatccccgcc gagatgcgaa aacgctccag gttcatgtgc gtggagctgc
     7201 cgatgccggc ggtcgtggcg acgaccgccc cggccttcgc cttggccgcg gcgaccaggt
     7261 ccgccacggt cttgtagctc ttggcggtcg agacgaccag caccgtcggg accgttccga
     7321 gagtcgcgac cggcgtgagc tccttcggat cgagcggtcc gggggaaagg agcggcgcca
     7381 cggtgaacgc ggaggtgtag atgctgatca cgtgtccgtc cggatcggcc ttggcgacat
     7441 aggcggcgcc gatcagcccg cccacgccca gcttgttctc gacgatcacc gcctggccga
     7501 ggctcgcctg caatttctcg gcgacgatgc gcgccgtcgt atccgaggcg ctgcccgggg
     7561 tgacgcctga aacgagatga atggtcctgc tgggaaacgt ctgcgcggct gcgatgcccg
     7621 atgcgagcga tgccccggca agcgcagtcc agcaaatgcg tttcaacatg gttttctcct
     7681 ctgttcggcc gtcagatcct gggcagcccc agctgcccgg caagccagtc ggccgtgcgg
     7741 cttcgatagc gataagggcg gttgttggca ttgtgcccgc cgtcctcgac gatcagcaac
     7801 tccaccggcc ccgagacgcc tttcgccagc cgctcggcgt gcgaggcggg caccagccgg
     7861 tcctcgcgcc cggtcacgat gaagatcggg caggcgattt tcgccgccgc ctctttcatc
     7921 gacagggtcg cggcgcggcg aagcgcatcg gcctcggtgg cgcaatggct gcgcacgcgg
     7981 aacgcctcgc gcgtcagcac cggcagcgcg ctccagattt ccttccactc gaacggcccc
     8041 gaaagcgcga tgcaggcttt gatgcgtttt tcgtaggcgg tggcgcgcgg cgcatagtag
     8101 ccgccgaggc tcacgcccca catggcgatc ttcgccgcgt cgagctcagg ctgctcttcg
     8161 atccagtcgc acaccgcatg cgcggcacgc tcgtagtcgc cgcagatcgg gatttcgtac
     8221 tcggcttcgc cctgcccggg cccgtcgatc gccagaatcg cgatacctcg cgcgagaaac
     8281 ggctccgcgt aggcctgcag ttcctccttg gtcgagtcca ggcccggtgc catgatgaga
     8341 atgggcaagc gccccgcacc ggccggtttt cgcagtaccc cgtacagcgt cttgccttcg
     8401 aagggaatcg cgacgcgctc gcccggcgga cgcacgtgcg gcagcgctgc cttgtagcac
     8461 tcg
//
LOCUS       DASPZD010000278         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_121972, whole genome shotgun sequence.
ACCESSION   DASPZD010000278 DASPZD010000000
VERSION     DASPZD010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121972"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..492
                     /gene="fabZ"
                     /locus_tag="VEM33_05410"
     CDS             58..492
                     /gene="fabZ"
                     /locus_tag="VEM33_05410"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HYS51294.1"
                     /translation="MDIREILEYLPHRYPMLLLDRVLECEPGKRLLALKNVSINEPFF
                     TGHFPHYPVMPGVMIIEALAQAAAVLSFRTMNTKADDKSVYYFAGIDDARFKRPVVPG
                     DQLLLEVAVERSARGLWKFAAQARVGDALAAEAKILCTLRPI"
     gene            489..1259
                     /gene="lpxA"
                     /locus_tag="VEM33_05415"
     CDS             489..1259
                     /gene="lpxA"
                     /locus_tag="VEM33_05415"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HYS51295.1"
                     /translation="MIHPTAIVHSGAKLAPGVSVGPYSIIGEHVEIGEGTSIGPHVLI
                     EGRTRIGAQNRIYGFSSLGGTPQDKKYAGEATALEIGDRNTIREYCTFSCGTAQDAGV
                     TRIGSDNWVMAYVHIAHDCRVGDHAVFANVATLAGHVQVGDYAVLGGFTGAHQFVSIG
                     AHSITGVATVLLQDLPPYVMAAGNTAKPFGINSEGLKRRGFAAAKITAIKRAYKTLYR
                     SGLALAEARRKIAGQAVKSPELQVLVDFLERSQRGIIR"
     gene            1265..2419
                     /gene="lpxB"
                     /locus_tag="VEM33_05420"
     CDS             1265..2419
                     /gene="lpxB"
                     /locus_tag="VEM33_05420"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HYS51296.1"
                     /translation="MDGKPIVVGMVAGEASGDFLGAHLIAALRARVPGLKFVGIGGPK
                     MEAEGLASWFPMEKLAVRGYVEVLRHFLELHAIRRKLRRRLLAAGPRLFIGIDAPDFN
                     LGLERRLKRAGIPAVHYVSPSLWAWRGGRMSAIARSVDKMLVLFPFEEPLYRAAGVPV
                     AYVGHPLADASSDEDESAAAKEQLRLPATRKVVALMPGSRQSEVRYMASSLIQTAKLI
                     AARQPAVHFLVPLVSRETRAIFEQAMYEAGAGELPLTVLFGHARDAIAACDVALVASG
                     TATLEAALARKPMVITYKMAQLTYGMMSKMGTLPWVGLPNIIAGEFLVPEILQDDATP
                     ENLAQAVLNSLNDPVVNRRLPERLDQIRRLLRRDTAARAAEALLPWLKSA"
     gene            2401..3090
                     /gene="rnhB"
                     /locus_tag="VEM33_05425"
     CDS             2401..3090
                     /gene="rnhB"
                     /locus_tag="VEM33_05425"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYS51297.1"
                     /translation="MAQVRLICGVDEAGRGPLAGPVCAAAVVFDPARRAPRGIADSKM
                     LSSHERERLTILIKDRALAWAIAWASVEEIDSLNILQASLLAMRRAVETLSVLPHEVL
                     FDGSHCPMLAVPARAIVDGDAKVRVIAAASILAKTARDAEMCRLHGRFPQYGFDVHKG
                     YPTPQHLLALRRYGVCEVYRRSFAPVREILDREGLGRGASSASSLRSPSRSAAGRNSG
                     RRAAVRRSRRA"
     gene            complement(3111..3554)
                     /locus_tag="VEM33_05430"
                     /pseudo
     CDS             complement(3111..3554)
                     /locus_tag="VEM33_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          604 a         1141 c         1216 g          593 t
ORIGIN      
        1 gccaagcgga tccgcgagct tgaaaagcga ctcccagtaa agagaaagag gtaagagatg
       61 gacatccgag agatcctgga atacttgcct caccgttatc ccatgcttct cctggaccgg
      121 gtgctcgaat gcgagccggg caagcggctt ctcgcgctca agaacgtctc cataaacgag
      181 cctttcttta ccgggcattt cccgcactat ccggtgatgc caggggtcat gatcatcgag
      241 gcgctcgccc aggcggcggc cgtattgtcg ttccgcacca tgaatacgaa agcggatgac
      301 aagtcggtct actacttcgc gggaatcgac gacgcacggt tcaagcgccc ggtggtcccg
      361 ggcgaccagc tcctgctcga ggtcgcggtg gaacgcagtg cgcggggact ctggaaattc
      421 gccgcgcagg cgcgcgtcgg cgatgcgctc gcggccgaag cgaagatctt gtgcacgctg
      481 cgcccgatat gattcacccg acggcaatcg ttcatagcgg agcgaagctc gccccgggag
      541 tatcggtggg accctattcg atcatcggcg agcacgtcga gataggggag gggacctcga
      601 tcgggccgca cgtgctgatc gagggaagaa ctcgcatagg ggctcagaat cggatttacg
      661 gattcagctc gctcggcgga accccccagg acaagaaata cgccggtgaa gcgaccgcgc
      721 tcgagatcgg cgaccgcaac accatccgcg aatactgcac cttcagctgc ggcaccgcgc
      781 aggatgcggg cgtcacgcgc atcggcagcg acaactgggt catggcctac gtgcatatcg
      841 cgcatgactg ccgggtcggc gaccacgcgg ttttcgccaa tgtggcgacg cttgccggtc
      901 acgtccaggt cggggactat gccgtgctgg gcgggtttac cggagcccat cagttcgtct
      961 caatcggcgc gcattcgatc accggggtcg cgacggtcct gttgcaggat ctgcctccgt
     1021 acgtcatggc cgccggcaat acggccaagc cgttcggaat caacagcgag gggctgaagc
     1081 ggcgtggctt cgcagcggcg aagatcacgg cgatcaagcg cgcctacaag accctctaca
     1141 ggtccggtct cgcgctcgcg gaagcgcggc gcaagattgc gggccaggcc gtgaagtcgc
     1201 ctgagctgca agtgctcgtc gatttcctcg agcgctcgca gcggggcatc atccgctagc
     1261 cgcggtggac ggcaagccca ttgtcgtagg catggtcgcg ggggaggctt ccggggattt
     1321 tctcggagct catctcatcg ctgcgttgcg cgcgcgcgtt cccgggctga agttcgtcgg
     1381 cataggcggc ccgaagatgg aggcggaggg cctggcttcc tggttcccca tggagaagct
     1441 cgcggtgcgc ggctacgtcg aggtgctgcg gcattttctg gaactccacg cgatccggcg
     1501 aaagctccgg cggcggctcc tcgcggcggg gccgagactt ttcatcggca tcgacgctcc
     1561 cgatttcaat ttggggctcg agcgcaggct gaagcgggcg ggaattccgg cggtccacta
     1621 cgtgagcccc tcgctttggg cctggcgcgg cgggcgcatg tccgcgatcg cgcggtcggt
     1681 ggacaagatg ctggtgctgt tcccgttcga agagcccctg taccgcgccg ccggagtgcc
     1741 ggtggcctat gtcggacacc ctctggcgga tgccagctcc gatgaggacg aatccgccgc
     1801 cgcaaaggag caattgcgcc tgcccgcgac gcggaaggtc gtggccctga tgccggggag
     1861 ccgccagtcg gaagtgcgct acatggcttc aagcctcata caaactgcca agttgatcgc
     1921 ggcgcggcag cccgcggtgc attttctggt gccgctcgtg agccgcgaaa cgcgtgccat
     1981 tttcgaacaa gcgatgtacg aagccggcgc gggggagctg ccgctcaccg tgctgttcgg
     2041 ccatgcgcgc gacgcgatcg cggcgtgcga cgtcgcgctg gtcgcgagcg ggactgcgac
     2101 gctggaggcg gcgctcgccc gaaagccgat ggtgatcacc tataagatgg cgcaactcac
     2161 ctacggcatg atgtcgaaga tgggaaccct gccgtgggtg ggccttccca acatcatcgc
     2221 cggagaattt ctcgtgcctg agattctcca ggatgacgcg acgcccgaga acctggccca
     2281 ggcggtgctg aactcgctca acgatccggt cgtcaaccgc aggcttccag agcgcctcga
     2341 ccagatacgg cggcttctgc gtagggatac cgcggcgcgt gccgcggaag cgctcctgcc
     2401 gtggctcaag tccgcctgat ctgcggcgtc gacgaagccg gccgcgggcc gctcgccggg
     2461 cccgtttgcg ccgccgccgt cgtattcgat ccggcgcggc gggctccgcg cggcatcgcc
     2521 gactccaaga tgttgagctc acacgagcgc gagcgcctca cgatcctgat caaagataga
     2581 gcgctcgcct gggccatcgc ctgggcgagc gtcgaggaga tcgacagcct gaacatcctg
     2641 caggcgagcc tgctcgccat gcgacgcgcg gtggagacgc tttcggtctt gccgcacgaa
     2701 gtgttgttcg acggctcgca ttgcccgatg cttgcagtgc cggcccgggc gatcgtcgat
     2761 ggcgacgcga aagtcagggt gattgcggcg gcgtccatcc tcgcgaagac cgcgcgcgat
     2821 gcggagatgt gtcgcctcca cggccgcttc ccgcagtacg gcttcgacgt gcacaagggg
     2881 tatccaacgc cgcagcacct tttggctttg cgcaggtacg gcgtatgcga agtctatcgc
     2941 cgcagcttcg ctccggtccg cgagatcctc gaccgcgagg gcctggggcg cggcgcgagc
     3001 tcggcgagca gccttcgcag tccttcccgc tccgcggcgg ggcgaaacag cggccggcgg
     3061 gccgccgtga ggcgctccag gcgcgcgtaa taggccgact ccagggccgc gcggcgtacc
     3121 tggcgcgcga gcgagcgctc gtcggcgagc cggtagtagc cggggtagcc gcggcccagc
     3181 atgccgacgt tcccggagac ccgggatgcg atcaccggaa cgcccgctgc ggccgcttcg
     3241 caaatgacgt tggcgccgtc ttccatgcgc gagctcacca ccagcgcgtg gctgcgcgag
     3301 agccagcgca tcgcctgcca atgcggcagg tttcccagcc agcgatagcg tggatcggca
     3361 cgctccagac gccgcgcggt gcgcgccatc tccggagaaa gtgcttcccc gagctgcacg
     3421 atctcgagct cgggcaaatc gcgcagatgg gcgagggcga gcgcggtgcg aaagggatcc
     3481 ttctcttccc gcaaatgtcc gatcaccgcg acgcgaaagc ggcgcgaggg cggtgggggg
     3541 gacgcgtcga cccg
//
LOCUS       DASPZD010000279         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_229534, whole genome shotgun sequence.
ACCESSION   DASPZD010000279 DASPZD010000000
VERSION     DASPZD010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229534"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VEM33_05435"
     CDS             <1..1238
                     /locus_tag="VEM33_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51298.1"
                     /translation="REELRQMMDGLLQDDSLRLELSRLSGFMQAMMPPSELSERYPFF
                     GEDPLSMGEAMQLMERLQQMDRLESQLERGSFRPDDVDRSLAQQLLGPEARQALDQLR
                     QVTDVLEKAGYVERKGRRLELTPRGMRRIGQSALRDIFDQLKKTRMGQHQLWRGGIGT
                     DASEELKAYEYGDPFLLEMKETLFNSIVREGPKVPVPIAPQDFIVHRTEHQTQASTVL
                     MIDMSRSMFLRGCFLAAKKVAIALDSLIRSQYPRDSLYVVGFSNYAVELKPHTLPQLA
                     LNDYVYGTNMQHGFQLGRSLLAKHRGNRQIIMITDGEPTAHIEDNGKAQFAYPPTYRT
                     IQQTLSEVKRCTRDRIVINTFMLERGPYLTEFINQMTKINKGRAFFVSPDRLGEYVLV
                     DYVSGKARRRSSARRARIA"
     gene            1275..2816
                     /gene="pckA"
                     /locus_tag="VEM33_05440"
     CDS             1275..2816
                     /gene="pckA"
                     /locus_tag="VEM33_05440"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR00224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HYS51299.1"
                     /translation="MASKTEVAVERRNLSPAALYEHAVRRDEAAIVSTGALTAETGKH
                     TGRSPKDKFFVKEPTSQDAIWWHPGNQPIPSDKFDGLLRRMEEFIATHEVYTQDVFAC
                     ADGRYRLRVRVITELAWHSLFARNLFIRPNADELLAFEPDFTVISLPSVKADTGRDGT
                     RSETFVLVNLGRRIVIIGGTGYAGEIKKSIFTALNYLLPAQNVFPMHCSANTNAAGDD
                     VALFFGLSGTGKTTLSAEPSRRLIGDDEHGWSDRGVFNFEGGCYAKTIRIHKESEPEI
                     YAATESFGTILENVVYDPRTRIPDYDSEEKTENTRAAYPIDLIPNAVLTGTGQHPHNV
                     MFLSADAYGVLPPVARLDEAQIRHYFLSGYTAKVAGTERGVTEPEPIFSTCFAAPFLV
                     LPPERYADMLIERVKRHHVDVWMLNTGWVGGPYGVGERMSIAHTRAIVRAILEGELRG
                     MSTHVDPVFGLQIPNRVPGVPREVLDTRDSWPSVEDYDRQAQKLRDMFDKNIGMIGKS
                     GSAAG"
     gene            2817..3167
                     /locus_tag="VEM33_05445"
     CDS             2817..3167
                     /locus_tag="VEM33_05445"
                     /inference="COORDINATES: protein motif:HMM:NF022151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HYS51300.1"
                     /translation="MKVAIVGSRHFSDLGRVTDYVKSLPARASIITGSASGVDAAATK
                     AAREKGVPVQVIPASFDELADPARSAARNQRLVDACDVLVAFWDRSSQGTRSTVDRAL
                     DSGKEVHVFIARVN"
     gene            3172..3429
                     /gene="moaD"
                     /locus_tag="VEM33_05450"
     CDS             3172..3429
                     /gene="moaD"
                     /locus_tag="VEM33_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HYS51301.1"
                     /translation="MAIAVGIRIRFFARLREQAGVETESIEVAPASTLADVYAALRGK
                     HAALPEQGAVRAALNQEFADWNTTVASGDEVAYIPPVSGGI"
     gene            3430..3858
                     /locus_tag="VEM33_05455"
     CDS             3430..3858
                     /locus_tag="VEM33_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HYS51302.1"
                     /translation="MRYDITTGPIDPRELEAAVTHSGAGAICTFSGIVRDNSRGQSVT
                     HLDYEAYAEMAVPQMRKIGQEIKDRWPEARVAMAHRTGHLEIGEVSVVVSVSCPHRAA
                     AFAACRWGIDELKQSVPIWKKEHAADGTHWIEGDEAKPVA"
BASE COUNT          769 a         1332 c         1302 g          667 t
ORIGIN      
        1 cccgtgagga gctgcgccag atgatggacg ggctcctgca ggacgactca ctccgcctcg
       61 agctgtcgcg cctgtccggc ttcatgcagg cgatgatgcc gccctccgaa ttgagcgagc
      121 gctatccgtt cttcggcgag gaccccctca gcatgggcga ggcgatgcag ctcatggagc
      181 ggctgcagca gatggatcgg ctcgagtcgc agctggagcg tggaagtttc cgcccggacg
      241 acgtcgaccg ctcccttgcg cagcagctcc tcggacccga ggcccgccaa gcgctggatc
      301 agctgcgcca ggtcaccgac gtcctggaaa aggccggcta tgtcgagcgc aagggccgaa
      361 ggttggagct cacgccgcgg ggaatgcgcc ggatcggcca atccgcgctg cgcgacatct
      421 tcgaccagct caagaaaacg cgcatgggcc agcaccagct atggcgcggc ggcatcggca
      481 ccgacgcctc cgaggaattg aaggcgtatg agtacggcga cccgttcctg ctcgagatga
      541 aggagacgct cttcaactcg atcgtgcgcg aagggccgaa ggtccccgtt cccatcgcgc
      601 cgcaggattt catcgtccac cgcactgagc accaaacgca ggcttcgacg gtgctgatga
      661 tcgacatgag ccgctcgatg tttcttcgcg gctgctttct cgccgccaag aaggtggcga
      721 tcgccctgga ttccctgatc cgcagccagt acccgcgcga ctctctgtat gtggtcggct
      781 tctcgaacta cgcggtggag ctcaagccgc atacgcttcc gcagctggcg ctcaatgact
      841 acgtgtacgg caccaacatg cagcacggct tccaactggg gcgttcgctg ctggcgaagc
      901 accgcggcaa ccgccagatc atcatgatca ccgacggcga gccgaccgct cacatcgagg
      961 acaacggaaa ggcgcagttc gcctatccgc cgacatatcg cacgatccag cagaccctga
     1021 gcgaggtcaa gcgctgcacg cgtgatcgca tcgtgatcaa caccttcatg ctcgagcgcg
     1081 gtccatacct caccgagttc atcaaccaga tgaccaagat caacaagggg cgggcgtttt
     1141 ttgtctcccc ggaccggctc ggcgaatacg ttctggtcga ctacgtgagc ggcaaggcgc
     1201 gacgccgctc gagcgcgcgg cgcgcccgca tcgcgtaagc ctttccccga cgccgttgct
     1261 tattctgaaa acagatggct tctaagaccg aagttgccgt cgagcgccgc aacctgagcc
     1321 cggcggccct ttacgagcac gcggtgcggc gagacgaagc cgcgatcgtg tccaccggcg
     1381 ccttgacggc tgagacaggc aagcacactg gacgctcgcc caaggacaag ttcttcgtca
     1441 aggagccgac gagccaggat gcgatctggt ggcacccggg caaccagccg atcccctccg
     1501 acaaattcga cggactgttg cgccggatgg aggagttcat cgcgacccac gaggtctaca
     1561 cccaggacgt gtttgcctgt gccgacggcc gctaccggtt gcgtgtgagg gtcatcaccg
     1621 agctggcttg gcacagcctg ttcgcgcgca acctgttcat ccgacccaac gccgacgagc
     1681 tcctcgcgtt cgagcccgac ttcactgtca tctcgctgcc ctccgtgaag gcagataccg
     1741 gacgcgacgg cactcgcagc gagaccttcg tgctggtcaa cctcggccgt cgaatcgtga
     1801 tcatcggcgg caccggatac gcgggagaga tcaagaagtc gatcttcacc gccctgaatt
     1861 acctgctgcc ggcgcagaac gtgttcccga tgcactgctc cgccaacacc aacgccgccg
     1921 gtgacgacgt cgcgctcttc ttcgggctga gcggcacggg taagacgacc ctgtcggcag
     1981 aaccttcgcg gcgcctcatc ggcgacgacg agcacggttg gagcgaccgc ggcgtcttca
     2041 acttcgaggg tggctgttac gccaagacaa tccggatcca caaggagtcc gagcctgaga
     2101 tctacgccgc gaccgaaagc ttcgggacga tcctcgagaa cgtggtctac gacccccgta
     2161 ctcggattcc cgattacgac tctgaggaga agacggagaa cacccgcgcg gcgtacccga
     2221 tcgatctgat tcccaacgct gtgctcacag gcaccggcca gcatccgcat aacgtcatgt
     2281 tcctttcagc cgacgcgtat ggcgtgctgc cgccggtggc gcgtctcgac gaggcccaga
     2341 tccgccacta ctttctctcc ggctacaccg ccaaggtcgc cggcacggaa cgcggagtga
     2401 ccgagccaga gccgatcttc agcacgtgct tcgcggcacc cttcctcgtc cttcctccgg
     2461 agcgctacgc cgacatgctg attgagcgag taaagcgaca ccacgtggac gtgtggatgc
     2521 tgaatacagg gtgggtcggc ggaccatatg gggtggggga gcggatgtcg atcgctcaca
     2581 ctcgggcgat cgtgcgcgcg attctcgaag gcgaattgcg tggaatgtcg actcacgtcg
     2641 acccggtttt cggcttgcag atccccaacc gcgtgcccgg cgtcccgcgc gaagtgctgg
     2701 acacgcgcga cagctggccc agcgtcgagg actacgaccg gcaggcgcag aagctgcgcg
     2761 acatgttcga caagaacatc ggcatgatcg gcaaaagcgg ctccgcggcc ggctgattga
     2821 aggtagcgat cgtcgggagc cgacacttct ccgatctggg gcgcgtgacc gattacgtca
     2881 agtcgctgcc cgcgcgggcc tcgatcatca cgggaagcgc cagcggcgtt gacgcggcgg
     2941 ccaccaaggc agcacgagag aagggcgttc cggtccaggt gataccggcc tcattcgacg
     3001 agcttgcgga tccggccagg tcggcggcgc gcaaccagcg cctcgtcgat gcatgcgatg
     3061 tgctggtcgc gttctgggac aggagctcgc aaggcacccg gagcacggtc gaccgcgccc
     3121 tcgactccgg caaggaagtc catgtgttca tcgcacgagt aaattgaagg cgtggcgata
     3181 gcggtcggga tcagaattcg ctttttcgcg cgattgcgcg agcaggcggg cgtggaaacg
     3241 gaaagcatcg aggtcgcgcc ggcgtcgacg ctggccgacg tttacgccgc gctccgcggc
     3301 aagcatgccg cgctgcccga acagggtgcc gtgcgagcgg ctttgaacca ggagtttgcg
     3361 gactggaata cgacggttgc cagcggcgat gaggtggcgt acatcccgcc ggtgagcggg
     3421 ggtatctgag tgcgttacga catcacgacc ggaccgatcg acccccggga gctggaagcg
     3481 gcggtgacgc actccggcgc cggtgcgatc tgcacgttca gcggcatcgt ccgcgacaac
     3541 tcacgcggcc agtccgtaac gcacctcgac tacgaggcct acgccgagat ggccgtgccg
     3601 cagatgcgca agatcggtca ggagatcaag gatcggtggc ccgaagcgcg ggtcgccatg
     3661 gcccaccgga ccggacacct cgagatcgga gaggtgtcgg ttgtggtctc ggtgtcatgc
     3721 ccgcatcggg ccgcggcctt cgccgcttgc aggtggggga tcgacgagct caaacagagc
     3781 gtgccgatct ggaagaagga gcacgcggcg gacggcacgc actggatcga aggagacgag
     3841 gcgaagcccg tcgcctagga cctgagcccc ctccggccct tcgggccgcc tccccagcaa
     3901 gtggggagga aagtgcctgg tcgcctagga ccttgccccc tccggccctt cgggccacct
     3961 ccccagcaag tggggaggaa actgggcgcc taggagctgg ccccctccgg cccttcgggc
     4021 cacctcccca acaagtgggg aggaaactcg tcgcttagga cctggccccc
//
LOCUS       DASPZD010000280         7371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_675196, whole genome shotgun sequence.
ACCESSION   DASPZD010000280 DASPZD010000000
VERSION     DASPZD010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675196"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEM33_05460"
     CDS             <1..448
                     /locus_tag="VEM33_05460"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYS51303.1"
                     /translation="SPGGTGFGSFLFRSFTDYIDLLACSVSSQAGAAALSVVKHPRLE
                     DHHVGCVLRVIPELLAVEGLDLSDIALFIPPQLSSEAIDELSAELKVDRGRFVHVSDG
                     GEDLFTSSVPYALEAVRCRGLAQPGEAGLIITTGAGIEVGCAIYYF"
     gene            485..1075
                     /locus_tag="VEM33_05465"
     CDS             485..1075
                     /locus_tag="VEM33_05465"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYS51304.1"
                     /translation="MRPLLTIYALHVFFWGVFVARLLFGFFSRATVAEGPRAEVPRAA
                     PEAGLLVSFHSLAIGLMYLALGFEQTRGGASMLFAPEPLVGAAIVLSATGLAAWALLW
                     FRSWRMSARLDRGHELATGGPFVLIRHPIYTSVNLVALSAFLWVPSWPVLAAVILVVI
                     GSDLRARAEERLLLEAFGDQYRAFMRGRRRFLPFVY"
     gene            1091..2215
                     /locus_tag="VEM33_05470"
     CDS             1091..2215
                     /locus_tag="VEM33_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS51305.1"
                     /translation="MRRIAIQSLLFDRGKLVASLLGVALATTLAFVQIGLYRGFQTNS
                     STIIQHVGGDFWLIPTGIDVLDNAQAMSPGPRNLLLSHPCVADARAVIYGFALIQKPS
                     GTRTTALIVAVEPRPGREVPWAVADGSVADLDQPLRISVDRTDLSKLELPPDAVGATF
                     EVSGRVVVVAAITDGIRSFTLSPYLFTSMTNGRRLLNLAESQAMYYSVDLADSGCAAE
                     LVGWIKGQRDVQLMETARWIEKTEDYWVGGSGAGMALAFTAVLGMIVGGVIIGQTLYS
                     MTKEHMLELATLKALGARSTELAGFVLWQVALLGSIGGAAGFAVALTLKRALAHAGLT
                     VVLSPETIALGSAATLAMCLLASITSLRAVFKVETAQVLR"
     gene            2212..2940
                     /locus_tag="VEM33_05475"
     CDS             2212..2940
                     /locus_tag="VEM33_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51306.1"
                     /translation="MTAGAVLVRTRNVSKTFRTGTIAAEALYEVSIELRAGELALLMG
                     PSGSGKTTLLYVLSGLLRPTGGEVELCGVPITRLPEENSAEVRRRHVGFIFQTYNLFP
                     ALTALDNVGEVLVMKGTPRAQARARAADILARVGLGDRLHYRPGELSGGQRQRVAIAR
                     ALAGDPTLVVGDEPTGALDRNTGRAIVRLLREQVSERRGVLLVTHDLRLRQFAHRIIE
                     IDDGRIVADYPVEETAEASGGDHA"
     gene            2933..4000
                     /locus_tag="VEM33_05480"
     CDS             2933..4000
                     /locus_tag="VEM33_05480"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYS51307.1"
                     /translation="MRKRLWTAVIAVAALGAAAAWWGAASRPGEQARAQAARPASEAR
                     VVAQAQIAAIDGIIEVRSLMEGKVLRVLVQPGDRVAANQLLAEIESGIPKAAVDQREA
                     DMKTASERLALAAEGVRPEDREALAAAAEAAKNEADLAQDRGQRQRRLFEQGFISQQA
                     LNEAERSALAAQARAREAEMRAKAGIAGGRPGEVRAAGEQLAAANAALNQGKIVLSRT
                     KIVAPIGGIIMTRSVNPGDIIGSNVTSPTLFKLVNPERVEVRLEVEELLASRVAIGLP
                     VTFVLPGGKVPVGQGKVTRIAPQVEKRTIGADDARVRADSMVRPAWSDFTPADKGDAF
                     PVNFRLEAWIQLFAGPQRSTD"
     gene            complement(3939..4988)
                     /locus_tag="VEM33_05485"
     CDS             complement(3939..4988)
                     /locus_tag="VEM33_05485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYS51308.1"
                     /translation="MFRIAGYSDTVGLWRHSKTLASSVRNHIVLDVLIALEKCGLLAE
                     LGTQHGVTPAALAGFDAVQLEAALEYLTEAEILEQVERGHFRVRKLREFPRLRSAVYA
                     AMAYNQPIRQLHRLIRGDDRYGVGISRDDQYDAMASEDITSRFYYGFTTRTLLGTNCA
                     SVLDLGCGTGAFPAYLAGHADLTEVIGVDVSDRAIAEGRRRGYESGPVRLLVGDAMAL
                     EITAAKLKTAPEIVSMMFVLHEFDDEGVGRILGSIHRAFPRAQILLTEMILRKTEEAL
                     ARNNTALPELRYVHQLSRQVIRSRDGWVHLFNRSNYRLSRRDEHQLSNTQCLVFSPSI
                     SAVRRRAESTPRGGS"
     gene            5371..6021
                     /locus_tag="VEM33_05490"
     CDS             5371..6021
                     /locus_tag="VEM33_05490"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HYS51309.1"
                     /translation="MRYRNSSRLSSTAAVAAVLGGLWVWPAISSAQLLPPLPVPTPIP
                     APVGTLLGATAVLGGTGTIVAGTSDVLQASDTTGGIASLLTGEALHAVTIGYPDQIDS
                     EASLAALALNVAGTSIGAAFVMSRATATASNAAGVSNIDGLSVNGAPIAVTGAPNQTI
                     GIPGGLLVLNEQQSLSDGTLVVNALHAIVSGVADVAVASAMAGLSGGSAKAVKASY"
     gene            6080..6646
                     /locus_tag="VEM33_05495"
     CDS             6080..6646
                     /locus_tag="VEM33_05495"
                     /inference="COORDINATES: protein motif:HMM:NF041523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-COAP-1 domain-containing protein"
                     /protein_id="HYS51310.1"
                     /translation="MRRTWTRYAFGVAALALALASGSANAWVCDFLTGGGFIVRDSGA
                     KANFGVGGGCKHGSPTWGHLEYIDHGMGLNVHWTSITAYMEIDSSADANGRPTGARFI
                     CGTARTNLFGDVDWVVAARDRGEPGVDDEFIIRLTQSGVILYTTEGDSDHSLGGSGPG
                     GGNIQLHKPNPSTTGSFGGSCPALFLEG"
     gene            6842..>7371
                     /locus_tag="VEM33_05500"
     CDS             6842..>7371
                     /locus_tag="VEM33_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51311.1"
                     /translation="MLKIGTRGRAAVLWAIFGLAWIPPQAFAQTNAGPARIEEADPSV
                     SYTVGWTQGDTSRTWSGGTAAVSTAPGAQATISFTGTSVSWIGGRTPGTGIARISLDG
                     IFLAEVDTYSKTEEIRVPMFRATGLPDARHTLTIEVTGQQNASATSNLIIVDAFDLPA
                     AAISRLQETDPSVTYTA"
BASE COUNT         1263 a         2390 c         2397 g         1321 t
ORIGIN      
        1 gagccccggt gggacgggat tcgggagctt cctcttcagg agcttcacag actacatcga
       61 cctgctcgcg tgctctgtca gcagccaggc gggcgcggcg gcgctctccg tcgtgaaaca
      121 tcccaggctc gaagaccacc atgtggggtg cgtgctgcgc gtcatccctg agcttctggc
      181 cgttgaaggg ctcgatcttt ccgacatcgc cctgtttatt ccgccgcagc tgtcgtcgga
      241 ggccatcgac gaactgagcg cggagctgaa ggtcgaccgc ggccgcttcg tccacgtgag
      301 cgacggcggg gaggaccttt tcacatcgtc cgtgccctac gcgctcgaag cggtgcggtg
      361 ccgagggctg gcgcagcccg gagaagccgg cctgatcatc actaccggcg ccgggatcga
      421 ggtcgggtgc gcaatctact atttctgacc cgcctgtcag acttcagatg aggaagctgc
      481 ttccatgcgg ccgttgctga ccatttacgc gctgcacgtg tttttctggg gcgttttcgt
      541 cgcgcgcctt ctttttggct ttttttcgag ggcaacggtc gccgagggac cgcgcgccga
      601 ggttccgcga gcggctccgg aggcgggcct tctggtaagc ttccacagcc tcgccatcgg
      661 tctcatgtac ctcgcccttg gctttgagca aaccagaggg ggcgcgtcca tgctctttgc
      721 tccggaaccc ctggtcggcg ccgcgattgt cctgtcggca accgggctgg cagcatgggc
      781 tctcctctgg ttccgttcgt ggaggatgag cgcccggctc gaccgcgggc acgagctcgc
      841 cactggcggg ccgttcgtcc tcatccgcca tccgatctac acgtcggtca atctggttgc
      901 gctcagcgcg ttcctctggg ttccaagctg gcccgtgctt gcggctgtta tcctggtagt
      961 aatcgggagc gacctgcgtg cacgcgcaga ggagaggctc ctcctcgagg cgtttggcga
     1021 tcaataccgc gccttcatgc gcggacgacg acggttcctg ccgttcgtgt actaaaatcg
     1081 cttcagagag atgcgacgga tcgccatcca gtccctgctt ttcgaccgcg gcaagctcgt
     1141 cgcttcgctg ctcggggtgg ccctcgccac gactctcgcc ttcgtgcaga tcgggctcta
     1201 ccgcggcttt cagaccaact cctcgacgat catccagcac gtcggcggcg acttctggtt
     1261 gatacccacc ggcatcgacg ttctcgacaa cgcgcaggcc atgtcgccgg gtccgcgcaa
     1321 tcttcttctc tcgcacccct gcgtggccga cgctcgcgcc gtgatctacg gctttgccct
     1381 gatccaaaaa ccgagcggca cgcgcacgac cgcgctgatc gtcgccgtcg agccccggcc
     1441 ggggcgggag gtgccatggg ccgtcgcgga cggatcggtc gcggacctcg atcagccgct
     1501 ccgcatcagc gtcgatcgca ccgatctctc caagctcgag ctgccgccgg atgcggtcgg
     1561 cgcgaccttc gaggtgagcg ggcgcgtggt cgtcgtcgcc gcgatcaccg acggcatccg
     1621 gtcgtttacg ctgagcccgt acctcttcac gtccatgacg aacgggcgaa ggctcctcaa
     1681 tctggccgag agccaggcga tgtactactc ggtcgacctc gccgattccg gctgcgccgc
     1741 cgagcttgtc ggctggatca agggccagag ggacgtgcag ctcatggaga cggcgcgctg
     1801 gatcgaaaag acggaggact actgggtggg cggctccggc gccggaatgg ccctcgcctt
     1861 caccgcggtg ctcggcatga tcgtcggcgg cgtgatcata ggccagaccc tttactcgat
     1921 gacgaaggag cacatgctcg agctcgccac gctcaaagcc ctcggcgcgc gctcgacgga
     1981 gcttgccggg ttcgtcctgt ggcaggttgc gcttctcgga tcgatcggcg gagcggccgg
     2041 cttcgcggtc gcgctcaccc tcaagcgggc gctcgcccac gccggcctca ccgtggtgct
     2101 gtccccggaa acgatcgcgc tcggcagcgc ggccacgctc gcgatgtgcc tgcttgcgag
     2161 cataaccagt cttcgcgccg tgttcaaagt cgagaccgcc caggtgctgc gatgacggcg
     2221 ggtgcggtcc tggtgcgcac gcggaacgtg tccaagacgt tccgcaccgg caccatcgcg
     2281 gcggaggcgc tctacgaagt atcgatcgag ctgcgcgccg gggagctcgc gcttctcatg
     2341 ggcccctccg gatcggggaa gacgacgctg ctctatgtcc tctcggggct gcttcgaccg
     2401 acgggcggcg aggtagagct ctgcggcgtg ccgatcacgc gtcttcccga ggaaaactcg
     2461 gcggaagtcc ggcgcaggca cgtgggcttc atcttccaga cgtacaacct tttccctgcg
     2521 ctcaccgcgc tcgacaacgt gggcgaggtg ttggtgatga aaggcacgcc gagggcgcag
     2581 gctcgcgcgc gcgctgcgga cattctcgcg cgggtgggtc tgggcgatcg cctccattac
     2641 aggccgggcg agctctcggg aggacagcgg cagcgggtcg cgatcgcgcg cgccctcgcg
     2701 ggcgatccga cgctcgtcgt cggcgacgag ccgaccggag cgctcgacag gaacacgggg
     2761 cgcgccatcg tgaggctgct gcgcgagcag gtgagcgagc gccgcggggt gctgctcgtc
     2821 acccacgacc tgcggctgcg gcagtttgcg caccgtatca tcgagatcga cgacggccgc
     2881 atcgtcgccg actatccggt cgaggagacc gcagaagcct ccgggggcga ccatgcgtaa
     2941 gcggttgtgg accgccgtta tcgctgtcgc cgcgcttggc gccgccgcag cgtggtgggg
     3001 cgccgcctcg cggcccggcg agcaagcgcg cgcgcaagcg gcacgtcccg cgtccgaggc
     3061 gcgcgtcgtc gcgcaggcgc agatcgccgc gatcgacggc atcatcgaag tgcggtccct
     3121 catggaaggg aaggtactgc gggtcctggt gcagcccggc gaccgggtgg cggcgaacca
     3181 gttgctcgcg gagatcgaaa gcggcatccc gaaggcggcc gtcgatcagc gggaagccga
     3241 tatgaagacc gcctccgaac gcctggccct ggcggccgaa ggcgtgcgtc cggaagatcg
     3301 cgaggccttg gcggccgccg cggaggccgc gaagaacgaa gccgacctcg cccaggatcg
     3361 cgggcagcgg cagcggcgcc tcttcgagca gggtttcatc agccagcaag cgctgaacga
     3421 agccgaacga agcgccctcg ccgcccaagc ccgcgcgcgc gaagccgaaa tgcgagccaa
     3481 ggccggcatc gccggcggcc gtccgggcga ggtgcgcgcc gccggcgagc agctcgccgc
     3541 cgcgaacgcg gcgttgaatc aaggcaaaat cgttctctcc cgcacgaaga tcgtcgctcc
     3601 catcggcggc atcatcatga cgcgcagcgt caatcccggc gacatcatcg ggtcgaacgt
     3661 gacttcgccg accttgttca agctcgtcaa ccccgagcgc gtcgaagtca ggctcgaggt
     3721 cgaggaattg ctcgcttcgc gcgtggcgat cggcctgccg gtcacgttcg tgctgccggg
     3781 cggcaaggtt cccgtcggcc agggcaaggt cacgcgcatc gcgccgcagg tcgagaagcg
     3841 cacgatcgga gcggacgacg cgcgcgtccg tgccgacagc atggtgcgcc cggcgtggag
     3901 cgatttcacc cccgccgaca agggcgacgc gttcccggtc aacttccgcc tcgaggcgtg
     3961 gattcagctc ttcgccggac cgcagagatc gacggactga agacgaggca ctgggtgttg
     4021 gagagttggt gttcgtcccg ccgactaagc cggtagttgc tccggttgaa caagtgtacc
     4081 cacccgtcac gggagcggat gacctgtcgt gaaagctgat gcacgtacct cagctcgggc
     4141 agtgcggtat tgttccgggc gagcgcctcc tcggtcttcc gaaggatcat ttcggtcagc
     4201 agaatttgag cccgtgggaa agcgcggtgt atggacccga ggatacgtcc gacgccttcg
     4261 tcgtcgaact catgcaggac aaacatcatt gacacgattt ccggggcggt cttcagcttt
     4321 gccgccgtga tctcgagggc cattgcatcc ccgacgagca ggcgtacagg tccggattca
     4381 tagcctcgcc ttcgtccctc ggcgatggcg cggtccgaca catccacgcc gatcacctct
     4441 gtcaggtccg cgtgccccgc gagatatgcc gggaaggcac cggtcccgca tccaagatcg
     4501 agcaccgagg cgcaattcgt ccccagcagc gttcgcgtgg tgaatccgta atagaagcgc
     4561 gacgtgatgt cttcggaagc catcgcgtcg tactggtcgt ccctggaaat tccgacgccg
     4621 tagcggtcat cgccgcggat cagccggtga agctggcgga tcggctggtt ataggccatc
     4681 gcggcgtaga ccgcagatcg cagccgggga aactcgcgga gcttgcgcac gcgaaaatgg
     4741 ccgcgctcga cctgctcgag gatctctgcc tctgtcaggt actccagcgc cgcttcaagc
     4801 tgcacggcgt cgaatccggc caaggcggcc ggcgtgacac cgtgttgcgt tcccagctcc
     4861 gccaggagac cgcatttttc cagggcgatc aagacgtcaa gcacgatgtg gttgcggacc
     4921 gaggatgcga gcgtcttgct atgtcgccac agtccgacag tatcactgta accggcgatg
     4981 cggaacatcg tccatacatc tcgcaagcgg ctcaatatgg tctttcctaa cagatggggc
     5041 cacttcccgg caacagagat acaacattaa tacaaattcg ctatttacca ctaagactcg
     5101 ctccgatccc cccgctgagg ggtacgcagg tggagcgaaa ttagcataag accgattgtg
     5161 tggttctaga caggttcaag ccggattgcg gcctgccgtc cggcttactt gaggcctcaa
     5221 ctcttgtaat aaagcgtggc caatctgttg catgtcggcc ctcttcgcaa cgaaggttgc
     5281 gcggatgata cgtgatcgga ttttttccac acaacaggaa gaagtatgaa tctttcaagg
     5341 cagcccgaaa ctgaaaacaa ggaggttact atgcgttaca gaaattcgtc ccgtttgagc
     5401 tcgaccgcag ccgtcgccgc agtcttgggc ggactgtggg tatggcctgc aatcagcagc
     5461 gcgcagcttc tcccccctct ccccgttccg actccgatcc ccgcgcccgt gggcaccctc
     5521 ctcggcgcga ccgcggtgct gggcggcacc ggaacgatcg tcgcggggac cagcgatgtt
     5581 ctccaggcgt cggatacgac aggaggcatc gcttccctgc tcacggggga agcccttcac
     5641 gccgtgacga tcggctatcc ggatcaaatt gactccgaag cctcgctcgc tgccctcgcg
     5701 ctgaacgtcg ccggaaccag catcggcgcc gccttcgtga tgtcgcgggc cactgcaaca
     5761 gcgagcaacg ccgccggcgt ctcgaatatc gacggcctct cggtcaacgg agcgccaatc
     5821 gcggtgaccg gagccccgaa tcagacgatc ggtatcccgg gaggcttgct ggtgctcaac
     5881 gagcagcaaa gcctatcgga cggcaccctc gtggtgaatg cgcttcacgc gatcgtctcc
     5941 ggcgttgcgg acgtggcagt cgcatccgcg atggccggtc tcagcggcgg aagcgcaaag
     6001 gcggtcaagg cgagctactg aaggctgagg tcttactggg aacgacctaa tcatcagaat
     6061 gcgaatggag gaaataacaa tgcgtagaac ttggacacgt tatgcgtttg gtgtcgcggc
     6121 gctcgcgctg gcgctcgcca gcggctccgc gaatgcctgg gtgtgcgact tcctgaccgg
     6181 cggcggcttc atcgtccgcg acagcggcgc caaggcgaac ttcggcgtcg gaggcgggtg
     6241 caagcacggc tcgccgacct gggggcatct ggagtacatc gaccacggca tgggcctgaa
     6301 cgttcactgg accagcatca cggcgtacat ggagatcgac tcgagtgccg acgccaacgg
     6361 caggccgacc ggggcccgct tcatttgcgg caccgcgagg acgaacctgt tcggtgacgt
     6421 cgattgggtc gtcgcagcca gggacagagg tgaaccgggc gtcgacgacg agttcatcat
     6481 ccggctgacg caatccggcg tcatcctcta tacgaccgaa ggtgattcgg atcacagctt
     6541 gggcggctcg ggccccggag gcggaaacat ccaactccat aaacccaacc cctcgaccac
     6601 gggatccttc gggggttcct gccccgcctt gttcctggag ggttagcggg tgcctgtcga
     6661 tctgcggggg agtctggacg acctgcatta ctcccctgca gcgatgcacg cagtgggtat
     6721 cggggccggg ggccggactt tatagttctt tcatagttcc ttcggcctgc ggcggatcac
     6781 tggctcttaa cagtcggggg tcatgatcag ggctactcaa gcggcgctga aggagcaaac
     6841 tgtgttgaag attgggacgc gcggccgcgc cgcagttcta tgggcgattt tcggcctggc
     6901 ttggatccct cctcaggctt tcgctcaaac caacgctggg ccggcgcgga tcgaggaggc
     6961 cgatccgtcc gtgagctaca cggtcggctg gacacagggc gataccagca gaacctggag
     7021 cggcgggacc gccgcggtct caacggcgcc gggcgcgcaa gcgacgataa gcttcactgg
     7081 gacttcagtc agctggatcg gcggccgcac gcccggcacc ggcatcgctc gcatatccct
     7141 ggatggaatc ttccttgccg aggtcgatac gtattcaaag accgaggaaa tccgcgtacc
     7201 catgttccgg gcgaccggcc tgcccgacgc gaggcatacg ctgaccatcg aagtgaccgg
     7261 ccagcaaaac gcgtccgcga cttccaacct tatcatcgta gacgccttcg acttgcccgc
     7321 cgcggcgatc tcgcgcttgc aagagacgga tccatccgtg acctacaccg c
//
LOCUS       DASPZD010000281         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1000001, whole genome shotgun sequence.
ACCESSION   DASPZD010000281 DASPZD010000000
VERSION     DASPZD010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000001"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VEM33_05505"
     CDS             complement(<1..502)
                     /locus_tag="VEM33_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYS51312.1"
                     /translation="MKFILHSLALLAAGFSCFAVAAEPPKIVMEEFMVPSKDPGIRLY
                     VRNKHPEGAKKFSAGKILLFVHGATYPAETSFDLQLNGQSWMDYIARRGYDVYLVDVR
                     GYGKSTRPPEMEQPAEQNAPIVRTETAVRDVGAAVDYIRQRRGVAKINLMGWSWGTSI
                     MGWYTAQ"
     gene            complement(563..1537)
                     /locus_tag="VEM33_05510"
     CDS             complement(563..1537)
                     /locus_tag="VEM33_05510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYS51313.1"
                     /translation="MKERFSIDRASFSGPVLVTGAGGCIGSWVLALLVRAGVPACALD
                     LSEDKRRPRLLIAESDLENIAWRTGDISESKTVAQVFETVRPCAVIHLAALQVPFCKA
                     DPVAGAKVNVVGTVNVFEAARRLGIARITYASSIAAYGAMDDGHGAMHTLYGAYKYCD
                     EQIAKVYSEDWKVASVGIRPGVVYGVGRDQGLTSKTTFAILAAAAGKPYEVPFSGGVS
                     WLYAGEVASAFIHAVSRERRGAPVFDMNGVYAPVEEGLRILRQLAPSAAITSSGQALA
                     FPMHLPDEPLRAQLGDYGNMPLAEGIRETYEAFQSLIRRGLVSMPESA"
     gene            1635..2696
                     /gene="ugpC"
                     /locus_tag="VEM33_05515"
     CDS             1635..2696
                     /gene="ugpC"
                     /locus_tag="VEM33_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HYS51314.1"
                     /translation="MASVTLSAVRKSFGSTEVVHGVDIGVEDGEFCVLVGPSGCGKST
                     LLRMIAGLEEISGGRIAIGERVVNGVAPKERDIAMVFQNYALYPHMSVYDNMAFSLKL
                     AGESREETRVRVEEAAMILGLMEYLRRYPRQLSGGQRQRVAMGRAIVRKPEVFLFDEP
                     LSNLDAKLRVAMRTEIKALHQRLKTTSVYVTHDQIEAMTMADKIVVMNAGRVEQIGSP
                     LELYDEPANQFVAGFIGSPAMNFLAGRLARNGAGFAVAVGGGVHLPIPVRAAIPEGRE
                     VVVGVRPEHFAVADDGVPAEVVVVEPTGADTQIFCKLAGVDVTAVVRERHAFQPGAAV
                     RLKPQLTYLFDPSSGARLA"
     gene            2706..4022
                     /locus_tag="VEM33_05520"
     CDS             2706..4022
                     /locus_tag="VEM33_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS51315.1"
                     /translation="MSNKRRDLLKAGAGLAASATLPMIWVKDAHAQWNSQPEKGAKLR
                     VLRWSRFVQGDIDQYMKNVQAFTAKYGIEVRVDSESWEDVRPKAAVAANTGAGPDIIL
                     GTYDDANLYPEKLVDVTDLCNYLGKKYGGWYPTCELYLKPDGKRWIGVPLGATGSMMV
                     YRESQVKAAGFQSFPKDTDGFLRMMKALKDKGTPGGFALGHATGDANTWCHWLVWTFG
                     GKLVDASNAVVINSPETLRALEYAKELYGTFIPGTLSWLDPNNNKVFLDGQISVTNNG
                     ISIYYASKNSKDPKINALQTDINHASYPSGPAGVSSACHLFMNQMIFKYTKYPKAAKE
                     FIRFMMEEEQYGAWLQAGIGYTAGPLVAYEKNPIWTVDPKHTAYRDACKQLRPAGYAG
                     KLGYASAGALADFIIVDMVAEAASGDKSPKEAMERAQKRAERYYKV"
BASE COUNT          797 a         1331 c         1362 g          679 t
ORIGIN      
        1 tctgcgcggt ataccagccc atgatgctcg tgccccaaga ccagcccatg aggttgatct
       61 tcgcgacgcc acggcgctgg cggatgtagt ccaccgccgc gccgacgtca cggaccgcgg
      121 tctcggtgcg cacgatgggc gcgttctgct ctgcgggctg ctccatttcc gggggacgcg
      181 tcgattttcc gtaaccgcgc acgtcgacga gatacacgtc gtagccgcgc cgggcgatgt
      241 aatccatcca cgactgcccg ttcagctgca ggtcgaacga ggtctcggcc ggataggtcg
      301 cgccgtgaac gaagagcaga atctttccgg cggagaattt cttcgctccc tccgggtgct
      361 tgttgcgcac gtagagccgg atgccggggt ccttcgacgg caccatgaat tcttccatga
      421 cgatcttcgg cggctcagcc gcgacggcga aacaagaaaa gcccgcagca agcaaagcga
      481 gcgaatgcag aatgaatttc atttttcccc ctgggatggt ggtcgcggaa ttctgcgcct
      541 tatttgcgcg ggccaagatg ccctacgccg actcgggcat gctcacgagc ccgcgccgga
      601 tgagcgactg gaacgcctcg taggtctcgc ggatgccttc ggcgagcggc atgtttccat
      661 agtctcccag ctgggcgcga agcggctcgt cgggaaggtg catgggaaat gcgagagcct
      721 gccccgaaga ggtgatcgcc gcagagggcg cgagctgcct gaggatgcga agcccctcct
      781 cgacgggcgc atacacgccg ttcatgtcga agacgggggc gcccctgcgc tcgcgcgaga
      841 cggcgtggat gaacgcgctc gcgacctcgc ccgcgtacag ccacgacacg ccaccggaaa
      901 aaggcacctc gtacggttta cccgccgcgg cggcgagaat cgcgaatgtc gttttcgagg
      961 tgaggccttg gtcacggccc acgccgtaga cgacccccgg cctgatgccg acgctcgcga
     1021 ccttccagtc ctcggaatac accttggcga tctgctcgtc gcagtacttg tacgctccgt
     1081 agagcgtgtg catggcgccg tggccgtcgt ccatcgcgcc gtaggccgcg atcgagctcg
     1141 cgtaggtgat gcgcgcgatc ccgagacgcc gcgcggcctc gaacacgttg accgtgccga
     1201 cgacgttcac tttggccccg gcgacggggt cggctttgca gaagggaacc tgcagagccg
     1261 cgagatggat caccgcgcaa ggacgcaccg tttcgaaaac ctgggcgacg gtcttgctct
     1321 ccgagatgtc gccggtgcgc cacgcgatgt tttccagatc gctctcggcg atcaagaggc
     1381 gcggtcgccg cttgtcctcc gacaagtcga gcgcgcaagc cggaacgccg gcgcgcacga
     1441 gcagcgcaag gacccagctt ccgatgcagc cgcccgcgcc ggtgacgagg acggggccgg
     1501 agaagctcgc gcgatcgatc gaaaaacgct ccttcattcg aatccccttg accgccacag
     1561 taggcgcggc taacctgccc tcgccctccg aaattttccc aaaaacgcga cgccccggca
     1621 aatcaagcac tcgcatggca tcggtgaccc tgagcgcggt gcgcaagagc ttcggctcga
     1681 ccgaagtcgt gcacggcgtt gacatcgggg tcgaggacgg cgaattctgc gtgctggtgg
     1741 gcccttcggg ttgcggcaag tctacgctct tgcggatgat cgcagggctg gaagagattt
     1801 caggcggccg gatcgcgatc ggcgagcgcg tcgtcaacgg cgtcgcgccg aaggagcggg
     1861 acatcgcgat ggtgttccag aactacgcgc tctacccgca catgagcgtg tacgacaaca
     1921 tggccttcag cttgaagctc gccggcgaat cgcgcgagga gacgcgcgtg cgggtcgagg
     1981 aggcggcgat gatcctcggg ctgatggagt acctgcggcg ctacccccgc cagctctcgg
     2041 gcggccagcg ccagcgcgtc gcgatggggc gcgcgatcgt aaggaagccc gaggtgttcc
     2101 tgttcgatga gccgctctcc aacctcgacg cgaagctcag ggtagcgatg cgcaccgaga
     2161 tcaaggcgct gcaccagaga ttgaagacga cgtcggtcta cgtgactcac gaccagatcg
     2221 aggcgatgac catggcggac aagatcgtgg tcatgaatgc gggccgcgtc gagcagatcg
     2281 gctcgcccct ggagctctac gacgaaccgg cgaaccagtt tgtcgcgggc ttcatcggat
     2341 cgccggcgat gaattttctc gccggccgct tggcgagaaa cggcgccggg ttcgctgtgg
     2401 cggtcggcgg cggcgtgcac ctgcccatcc cggtgcgcgc cgcaatcccc gagggccgcg
     2461 aagtggtggt cggcgtgcgg cccgagcatt tcgcggtagc cgacgacggc gtgcccgcgg
     2521 aagtggtggt ggtcgagccc accggcgccg acacgcagat cttctgcaag ctcgccggcg
     2581 tcgacgtcac ggcggtggtg cgcgagcgcc acgcctttca acccggcgcg gcggtgcgcc
     2641 tcaagccgca attgacctat cttttcgatc cgtcgagcgg cgctcgactc gcataaggag
     2701 gagccatgag caacaagcgc agggacttac tgaaagcagg agcaggcctc gccgcgagcg
     2761 cgacgctgcc gatgatctgg gtgaaggacg cgcacgccca gtggaacagc cagcctgaga
     2821 agggcgcgaa gctgcgggtg ctgcgctgga gccgcttcgt ccagggcgac atcgaccagt
     2881 acatgaagaa cgtgcaggcg tttaccgcca agtacggcat cgaagtgcgc gtcgacagcg
     2941 agagctggga agatgtgcgc ccgaaggcgg cggtcgcggc caacaccggc gccggaccgg
     3001 acatcattct cggcacctac gacgatgcca atctctatcc cgagaagctg gtggacgtca
     3061 ccgacctttg caactatctc ggcaagaagt acggcggctg gtacccgacg tgcgagctct
     3121 atctcaagcc cgacggcaag cgctggatcg gcgtgccgct cggcgcgacc ggcagcatga
     3181 tggtctaccg cgagagccag gtgaaagcag cgggattcca gagctttccg aaggacaccg
     3241 acggcttcct caggatgatg aaagcgctca aggacaaggg cacgcccggc ggcttcgcgc
     3301 tcggccacgc caccggcgat gccaacacct ggtgccactg gctggtgtgg accttcggcg
     3361 gcaaactggt cgacgcgagc aacgccgtcg tcatcaacag cccggaaacc ctcagggcac
     3421 tggaatacgc gaaggagctc tacggcacgt tcatcccggg cacgctgtcg tggctcgatc
     3481 cgaacaacaa caaggtcttc ctcgacgggc agatctcggt gaccaacaac ggcatctcga
     3541 tctattacgc gtcgaagaat tccaaggacc cgaagatcaa cgcgctgcag accgacatca
     3601 accacgcgag ctatccgtcg gggccggccg gcgtgtcgag cgcctgccac ctgttcatga
     3661 accagatgat cttcaagtac acgaagtatc cgaaggcggc caaggaattc atccgtttca
     3721 tgatggagga agagcaatac ggcgcatggc tgcaggcggg aatcggctat accgcgggcc
     3781 ctctcgtcgc ctacgagaaa aacccgattt ggacggtgga cccgaagcac acggcctacc
     3841 gcgacgcctg caagcagctg cgtcccgcgg ggtatgccgg caagctcggc tacgcgtctg
     3901 caggcgcgct cgccgacttc atcatcgtgg acatggtcgc cgaagccgcc tcgggcgaca
     3961 agtcgccgaa ggaagccatg gagcgcgcgc agaaacgcgc cgagcggtat tacaaggttt
     4021 gacttttaaa cctttcccct cctccatcct ggaggagggg tgtcgccgga ggcgacgggg
     4081 tggtggtgtg aacatgcatc aaaccacccc gcgcgcttcg cgcgcacccc tccttgtcca
     4141 aggaggggat gacactctga cccgtgcat
//
LOCUS       DASPZD010000282         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1763983, whole genome shotgun sequence.
ACCESSION   DASPZD010000282 DASPZD010000000
VERSION     DASPZD010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763983"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1565)
                     /gene="lysS"
                     /locus_tag="VEM33_05525"
     CDS             complement(75..1565)
                     /gene="lysS"
                     /locus_tag="VEM33_05525"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HYS51316.1"
                     /translation="MTEELNFVLQARREKLEALEAAGVAPFAYGFDRQHTAEQAHSML
                     AAGEHDGPEVSLAGRIVAWRSHGKTTFAHLADPTGKIQLYFKKDQLGEKPYSLLAHFD
                     LGDIIGVHGPLFRTKTGEITVRVDRVELLAKSLRPLPFGKEEEVNGQIVRHSGFTDPE
                     QRYRQRYADLAVHPEVRALFVARSRMTSAIRGFLDERDYLEVETPILQPLYGGAAARP
                     FTTVHNALDMPLYLRIADELYLKRLVVGGLERVYEIGHDFRNEGIDRTHNPEFTMLEF
                     YQAYADYSSMMEIVEQLLEKTAEAVRSVPELRANVPNFERPFKRVEWFPSLNSAAGTD
                     VSALDDAKLRALAARVGVEQPESMSHPKLLDEMFQALVESKLVKPTFVVDYPVELSPL
                     AKPKRGNPTLTERFELFANGKELANAFSELNDPLDQRRRFEAQAHLRAAGDLEAAGVD
                     EDYLRAMEYGMPPMGGVGIGMDRLLMYLSGASNIRDVILFPTMRPE"
     gene            complement(1562..2681)
                     /gene="prfB"
                     /locus_tag="VEM33_05530"
     CDS             complement(join(1562..2608,2610..2681))
                     /gene="prfB"
                     /locus_tag="VEM33_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139687991.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HYS51317.1"
                     /translation="MAESERSRGLKQSRDRLAELRRYLDIDGKRARLSVLDAEMADPA
                     FWNSQEKAQATLQEAKTLRGWIEPHDKLNDRLVSALELDELLQDSPEPAMERELDAEI
                     SQLRDEIGSFEVKSLLRGPDDFRDAQLEISAGAGGTEAQDWAQMLMRMYTRWAERRGF
                     EVSILDESEGEEAGIKGVVLEIRGQYAYGFLRPETGVHRLVRISPFDANARRHTSFAS
                     VFVYPVVNEEINIDIRDEDLRIDVYRASGAGGQHVNKTSSAVRITHIPTGTVVASQQE
                     RSQFKNKSTAMKMLKNKLYQAEIERREAHKAALDATKSDVSFGSQIRSYVFQPYTMVN
                     DHRTELKIPDVQKVMDGGIDPFIEAYLKQGGGSATAAA"
     gene            complement(2701..3081)
                     /locus_tag="VEM33_05535"
     CDS             complement(2701..3081)
                     /locus_tag="VEM33_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51318.1"
                     /translation="MPPFIPSTPASRPNPPRPPAPPATPPGASAAIGDGGARRPLNPF
                     LSKDPNQRAKRLARALVSDIITYHPAKHAEGLRDGTLKQLFREEIKKSFEEYIAQVGQ
                     QLAESTTYFQEALNEVLAAGKKIF"
BASE COUNT          616 a         1100 c         1066 g          704 t
ORIGIN      
        1 gctgatcagc gacagcagct ttgatccgcg acgactgcgg aggtaccgcc aggctatttg
       61 taattcgagc ttgctcattc cggcctcatc gtcggaaaca gaatcacatc ccggatgttc
      121 gacgcacccg acagatacat cagcaaccgg tccatcccaa tacccacacc acccattggc
      181 ggcattccgt attccattgc ccgcaagtag tcctcgtcga cgccggcggc ctccagatca
      241 cccgccgcgc gcaggtgcgc ctgggcctcg aaacgacgac gctggtcgag cggatcgttc
      301 agctcactga acgcgtttgc gagctctttg ccattcgcaa acagctcgaa ccgctcggtg
      361 agcgtcggat ttccccgctt cggtttcgca agcggcgaga gttccaccgg atagtccacg
      421 acaaacgtcg gcttgaccaa ttttgactcc acgagcgcct gaaacatttc atccaacagc
      481 ttcgggtgac tcatcgattc tggttgttct accccgacac gcgccgcaag tgcacgcagc
      541 ttggcatcgt caagtgcgga aacgtcggtc cccgccgctg agttgagcga cgggaaccat
      601 tcaacgcgct tgaacgggcg ctcgaagttc gggacgttgg cgcgcagctc cggcacggat
      661 cgcacggcct ccgccgtttt ctcgagcagc tgctcgacga tctccatcat gctcgagtaa
      721 tcagcgtacg cctggtagaa ctcgagcatc gtgaactcag ggttgtgcgt ccgatctatg
      781 ccctcatttc taaagtcgtg accgatttcg taaacgcgct cgagccctcc caccaccagt
      841 ctcttcaaat aaagctcgtc ggcaatgcgc aggtaaagcg gcatgtcgag cgcgttatga
      901 accgtcgtaa atggccgcgc cgccgcgcca ccgtagagcg gctgcagaat aggcgtttct
      961 acctccaggt aatcgcgctc gtcgaggaac ccgcgaatag cactcgtcat gcgcgaacgc
     1021 gccacaaaaa gcgcccgtac ttccgggtgc acggcgagat ccgcataacg ctgccggtat
     1081 ctctgttcgg gatcagtgaa ccccgagtgg cggacgatct ggccgttgac ctcttcttcc
     1141 ttcccgaagg gaagcggacg aagcgactta gccaacagct ccacgcgatc gacacgcacc
     1201 gtgatttccc ccgtcttcgt ccggaagagc gggccgtgga cgccgataat gtcaccgaga
     1261 tcgaagtgcg ccagaagaga gtacggcttc tcgccgagct gatccttctt gaaatagagc
     1321 tgaatcttgc cggtcggatc ggcgagatgc gcgaacgtcg tcttgccgtg cgaccgccag
     1381 gcgactatgc gtcccgcgag actgacctcc ggtccgtcgt gctcgccggc tgccagcatc
     1441 gagtgcgctt gctccgccgt gtgttgccgg tcaaaaccgt acgcgaacgg cgctacgccc
     1501 gccgcctcca gggcctcgag tttctcgcgt cgggcctgga gaacgaagtt gagttcttcg
     1561 gtcacgcggc cgccgtcgcg gagccgccgc cctgctttag atatgcctcg atgaacgggt
     1621 cgattccgcc gtccatcact ttctgaacgt caggaatctt caattcggta cgatggtcgt
     1681 tgaccatcgt atagggctgg aacacgtagc tcctgatctg gctcccgaag ctcacatcgg
     1741 acttcgttgc atccagtgcc gccttgtgcg cctcacgtcg ctcgatctcg gcctgataga
     1801 gcttgttctt cagcatcttc atcgccgtcg acttgttctt gaactgcgag cgctcctgct
     1861 gtgaggcgac aacggtgccg gtgggtatgt gcgtgattcg cacggcggag ctggtcttgt
     1921 tgacgtgttg tccgccggcg cccgatgcgc ggtaaacgtc gatcctcaga tcctcgtcac
     1981 ggatgtcgat gttgatctct tcgttcacaa ccggatacac gaataccgat gcgaagctgg
     2041 tatgccgccg cgcgttggca tcgaacggcg aaatccggac gagacgatga actcccgtct
     2101 cgggacgcag aaagccgtaa gcgtactgcc cgcgaatctc cagcacgacg cccttgatgc
     2161 cggcctcctc accctcactc tcgtcgagga tgctgacctc gaagccacgg cgctccgccc
     2221 accgggtgta catgcgcatc agcatctgcg cccagtcttg cgcctcggta ccaccggcac
     2281 cggcgctgat ttcgagttgc gcgtcgcgga aatcatccgg cccacgcaaa agcgacttca
     2341 cctcgaagga accgatctcg tcccgcagct gcgaaatctc cgcgtccagc tcgcgctcca
     2401 tcgccggctc ggggctgtcc tgcaacagct cgtcgagctc gagagcgctc accagtcggt
     2461 cattcagttt atcgtggggt tcgatccagc cgcgcagcgt tttcgcttcc tgcagcgtcg
     2521 cttgcgcctt ttcctggctg ttccagaagg cggggtcagc catttcggcg tcgagcacgc
     2581 tcaggcgagc gcgcttgccg tcgatgtcaa agatacctcc gcaactccgc cagtcggtcc
     2641 ctgctctgct tcaggccacg gctgcgttca ctttccgcca tttatctggg cctccggccc
     2701 ttagaagatt ttcttgcccg ctgccagcac ttcgttcagt gcttcctgaa aataagtggt
     2761 gctctctgca agctgctgac ccacctgcgc aatatactcc tcgaagctct tttttatctc
     2821 ctctctaaag agctgcttca gagtcccgtc acgaagccct tcggcgtgct tcgctggatg
     2881 gtaggtgatg atgtcggaga ccagcgctcg tgcgagcctc ttggcgcgct ggttgggatc
     2941 cttggagaga aacgggttga gcgggcgacg cgcgccgcca tcgccgatcg ctgcggatgc
     3001 gccgggcggc gtagcaggag gggccggagg gcgcggcgga tttgggcgac tcgccggagt
     3061 tgacggaatg aacggcggca ccgatccaac cggggcggaa gaggtcggag gcgtgaaggg
     3121 tgaaaccggt ggcggggccc cggcaccagg aggggtgaac ggcggtacag gcggtggtgc
     3181 agcgaattca ggcagactgg tggcgggcga tggtggcgtt gccgtcgccg taaccggtcc
     3241 gcggtcgagc ggctcctcgc gcaccggacg cggcgcagcc ggtatttcgg ctgtgcgcat
     3301 ggggggcgga ggcgcaggag gggtcgggcg ctgggtgggc ggggccgccg gtgtctgacg
     3361 tgcagaggta ccaaacggcg gtgagtccca gccactctgt gcaggaggag tcgagcgtgg
     3421 ccgctcgact ggggcgggcg gagtgttggt gcgctggccg ccgccggatg gagtggtctg
     3481 gctcga
//
LOCUS       DASPZD010000283         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1772022, whole genome shotgun sequence.
ACCESSION   DASPZD010000283 DASPZD010000000
VERSION     DASPZD010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772022"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /gene="ettA"
                     /locus_tag="VEM33_05540"
     CDS             complement(<1..1650)
                     /gene="ettA"
                     /locus_tag="VEM33_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HYS51319.1"
                     /translation="MAQYVYTLRRVGKLVPPKRQILKNISLNFFPGAKIGVLGLNGSG
                     KSTLLKIMAGVETNFEGEVTAMPNMKVGFLPQEPQLDAEKTVREAVEEGLGETLAAKK
                     RLEEIYAAYAEPDADFDKLAAEQAKLEAIVNAVHADSADLQMEVAADALRLPPWEQKI
                     APLSGGEKRRVALCRLLLSKPDILLLDEPTNHLDAESVEWLEQFLRRFPGTVVAVTHD
                     RYFLDNAAQWILELDRGYGIPWEGNYSSWLEQKEKRLEIEEKQEQARIKSMQKELEWV
                     RQNPKGRQAKSKARLQRFEELSSYEYQKRNETQEIFIPVADRLGDSVIEFKGVSKGYG
                     DRLLIDDLSFSAPPGAIVGIIGPNGAGKSTLFRMITGKEKADRGKIVIGPTVKMAHVD
                     QSREALEADKTVWEFISAGADILTVGKFEMPSRAYLGRFNIKGADQQKKLGTLSGGER
                     GRVHLAKTMLAGGNVLLLDEPSNDLDVETLRALEEALIEFAGCVLVISHDRWFLDRIA
                     THILAFEGESKVAFFAGNYQEYESDKIKRLGEEAAKPKRIRY"
     gene            complement(1694..2554)
                     /locus_tag="VEM33_05545"
     CDS             complement(1694..2554)
                     /locus_tag="VEM33_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYS51320.1"
                     /translation="MNLTALQIFKSVVEQGGVNKAAAKLHRVPSNVTTRIKQLEEQLG
                     AKLFLRIGRRLALSAEGKLLLPLADQLLRLSSEAEAAVRNEKPGGTLRIGALESTAAS
                     RLPPVLSRYHQLYPDVQIELVTGTSGALVSRVHKEEVEAAFVAEPFNANGLEIQTAFV
                     EELVLITPKSFGQVRSAKDIGNRTVIAFSAGCSYRQRLETWLGASKVLPDRVMEFQSY
                     HAIIACVAAGSGLAVVPRAVLELTRADRNVAMTALPARIAKTRTQLVWRREHHSAALD
                     AMKNLFAGKH"
BASE COUNT          450 a          882 c          864 g          552 t
ORIGIN      
        1 gtagcggatg cgcttgggtt tggccgcctc ttcgccgagg cgcttgatct tgtccgattc
       61 gtattcctgg tagttgcccg cgaagaacgc aaccttggac tcgccttcga aggcgaggat
      121 gtgagtggcg atgcggtcga ggaaccagcg gtcgtggctg atgacgagca cgcagccggc
      181 gaattcgatg agcgcttcct cgagcgcccg cagcgtttcc acgtccaggt cgttcgacgg
      241 ctcgtcgagc agcaggacgt tccctccggc gagcatcgtc ttcgcgaggt gaacccggcc
      301 gcgctcgccg ccggagagcg tgcccagctt cttctgctgg tcggcgccct tgatgttgaa
      361 ccggccgaga tacgcgcgcg aaggcatctc gaacttgccc acggtgagga tgtcggcgcc
      421 cgccgagatg aattcccata cggtcttgtc ggcctcgagc gcttcgcgcg actgatccac
      481 gtgggccatc ttcaccgtcg gcccgatgac gatcttgccc cggtccgcct tttcctttcc
      541 ggtgatcatg cggaacagcg tcgatttccc ggcgccgttc gggccgatga tgccgacgat
      601 cgcaccgggc ggcgcactga acgacaggtc gtcgataagc aggcgatcgc cgtagccctt
      661 ggaaacgccc ttgaactcga tcaccgaatc gccgaggcgg tccgcgaccg ggatgaaaat
      721 ctcctgggtc tcgttgcgct tctggtattc gtacgaggac agctcctcga agcgctggag
      781 gcgcgccttc gatttcgcct ggcggccctt ggggttctgc cgcacccatt cgagctcctt
      841 ctgcatcgac ttgatgcggg cctgctcctg cttctcttcg atctccaggc gcttctcctt
      901 ctgctcgagc caggaggagt agttgccctc ccagggaatg ccgtagccgc ggtcgagctc
      961 gaggatccac tgggccgcgt tgtcgaggaa gtagcgatcg tgcgtcaccg cgaccaccgt
     1021 gccgggaaac ctccgcagga actgctcgag ccactcgacg ctctccgcat cgaggtggtt
     1081 ggtgggctca tcaagcagca ggatgtcggg cttcgagagc agcaaccggc acaacgcgac
     1141 gcggcgcttc tcgccgccag agagcggcgc gattttctgc tcccacggcg gcaagcgcag
     1201 cgcgtcggcg gcgacctcca tctgcaggtc cgcgctgtcc gcgtgcacgg cgttgacgat
     1261 cgcctcgagc ttcgcctgct cggcggcgag cttgtcgaaa tcggcatcgg gctcggcgta
     1321 agccgcgtag atctcctcga ggcgcttctt tgcggcgagc gtttcgccga gaccttcctc
     1381 caccgcttcg cgcaccgttt tttccgcgtc gagctgcggc tcctgcggca ggaagccgac
     1441 tttcatgttc ggcatcgcgg tcacttcgcc ctcgaaattc gtctcgacgc cggccatgat
     1501 cttcagaagc gtggatttcc ccgagccgtt caggccgagc acgccgatct tcgcgccggg
     1561 aaagaaattg agcgagatgt tcttgaggat ctggcgcttg ggcggcacga gcttgccgac
     1621 ccggcgcagg gtgtatacgt attgagccat agggcgcgaa gtctacagca agtgccgctc
     1681 ccgatcgtcc gtgctagtgc ttccccgcaa acagattctt cattgcatcc agtgcggccg
     1741 aatggtgttc gcggcgccag acgagctgcg tccgcgtctt tgcgatcctc gcgggcaggg
     1801 cggtcatcgc cacgttccgg tccgcacgcg tcagctcgag gacggcgcgc ggaacgaccg
     1861 cgaggccgga cccggcggcc acgcaggcaa tgatcgcgtg atacgactgg aattccatga
     1921 cgcgatccgg caggaccttg cttgcaccga gccaggtctc gagccgctgc ctgtacgagc
     1981 agcccgccga aaatgcaatc acggtccgat ttccgatgtc tttcgccgaa cgcacctggc
     2041 cgaagctctt cggcgtgatg agaacgagct cttcgacgaa cgccgtttgg atttcgagac
     2101 cattggcgtt gaacggttcc gcgacgaaag cggcttctac ctcctccttg tgcacccggg
     2161 agaccagggc gccggaggtg cccgtgacca gctcgatctg gacgtccgga tagagttgat
     2221 ggtagcggga cagaacgggg ggcagccggc tcgctgcggt gctttccagc gccccgatcc
     2281 gcagggttcc cccgggtttc tcgttgcgca ccgcggcttc ggcctccgag gaaagtcgca
     2341 gcagttggtc ggcgaggggg aggagcagct tgccctcggc ggacaacgca agccggcgac
     2401 cgattctcag gaagagcttg gctccgagct gctcttcgag ctgtttgatc cgggtcgtaa
     2461 cgttcgaggg cacgcggtgc aacttcgccg cagccttgtt gacgccgcct tgctcgacca
     2521 cggatttgaa gatctggagc gcggtgagat tcatattctc aataagagaa cgtattattc
     2581 ataattattc acttttatct ctaggacagc caagttacac tgcttcggtg cggcacgcaa
     2641 cacccgacat cgaacacggc tcagtctccg gctcagcctg ggccagcacc gttgcggccg
     2701 tggccgcact cgccgtcgcc atgggcatcg ggcgcttcgc attcaccc
//
LOCUS       DASPZD010000284         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_749407, whole genome shotgun sequence.
ACCESSION   DASPZD010000284 DASPZD010000000
VERSION     DASPZD010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749407"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VEM33_05550"
     CDS             <1..908
                     /locus_tag="VEM33_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51321.1"
                     /translation="MVAFGGGGNWPVWAAQEKGLFAKNGIAVKLSFTPNSVEQIRNLM
                     NGTYDLGTTAYDNVVAYQEGQGETELATQPDLFAFMGGYSGSLRFVTQPDIKTYAALK
                     GKTVGVDAATTGFAFILYKLAALNGLGMSDYKVEKLGGTPARVQAMMEGRIAGTMISS
                     PSEILPESKGYTRLGDTTTTFGPYQASIGVARRSWAAKNGDQLIAFIRSYAAAMDWLQ
                     QPANKDEAVAIYVKYIPSAPRPAAQKAYEVMFGEKEGFQKKAKLDIEGCRTVLKLRSE
                     FAKPQKNLTDPAKYIDESFYSKAVQ"
     gene            929..1612
                     /locus_tag="VEM33_05555"
     CDS             929..1612
                     /locus_tag="VEM33_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYS51322.1"
                     /translation="MSHAKSTVSAALVVAATAGLAAGCQTTDPYTGEMKTSSATFGAL
                     IGAGTGAAIGMISGHDAKQRRQRALVGAGVGALAGGAVGGYMDKQESELRALMRDSGV
                     TVTRKGEDIVLNMPGNVTFRTGSADLNAQFFKVLDGVAQVARKYDKTIIEIAGHTDNV
                     GGTDFNRQLAQRRASTVARYLTSKGVSEPRIMTAAGGEDHPIASNSTEQGRAANRRVE
                     VTLAPLKEG"
     gene            complement(1625..>2116)
                     /locus_tag="VEM33_05560"
     CDS             complement(1625..>2116)
                     /locus_tag="VEM33_05560"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HYS51323.1"
                     /translation="WLILKTEGELQKKALAWARWGLLWVALGVALVSLATPLVSHTVQ
                     AKWFDFPRTLALMLLPLATLAAGWRVWAATGRLKRGEREFDWMPLAATVAIFTLAFAG
                     LAYSLFPYVVIDRMTIWEAASHPSALKFMLVGVAIVFPFLIAYTAFAYRVFRGKARAG
                     MYE"
BASE COUNT          437 a          699 c          690 g          290 t
ORIGIN      
        1 agatggtcgc cttcggcggc ggcggcaact ggccggtctg ggccgcgcag gagaagggcc
       61 tcttcgcgaa gaacggcatc gccgtgaagc tttccttcac gccgaattcc gtcgagcaga
      121 ttcgcaacct gatgaacgga acctacgacc tcggcaccac cgcgtatgac aacgtcgtcg
      181 cctaccagga aggacagggc gagaccgagc tcgcgacgca gcccgacctt ttcgccttta
      241 tgggcggcta ttcgggatcg ttgcgcttcg tgacccagcc ggacatcaag acgtatgcgg
      301 cgctcaaggg caagacggtc ggcgtggacg cggcgaccac ggggttcgcc ttcatcctct
      361 acaagctcgc ggcgttgaac ggccttggca tgtccgacta caaggtcgag aagctcggcg
      421 gcaccccggc gcgcgttcag gcgatgatgg aaggccggat cgccggaacc atgatcagct
      481 cgccgtcgga gatattgccc gagtcgaagg gctatacgcg gctgggcgac acgactacga
      541 ctttcggtcc ctaccaagcc tccatcgggg tcgcgcgccg ctcctgggcg gcgaagaacg
      601 gcgaccagct gatcgctttc atccgctcgt atgcggccgc gatggactgg cttcagcagc
      661 cggcgaacaa ggacgaggcc gttgccatct acgtgaagta catccccagc gccccgcgcc
      721 ccgcggcgca gaaggcctac gaagtgatgt tcggggagaa ggaaggcttc cagaagaagg
      781 cgaagctcga catcgaaggc tgccgcaccg tgctcaagct tcgcagcgag ttcgccaagc
      841 cccagaaaaa tctcaccgat cccgcgaagt acatcgacga gagcttctac agtaaagcag
      901 tgcagtagcg tcaaaggaga cccaagacat gtcccatgca aaatccacgg taagcgcagc
      961 cctggtggtg gcggctaccg ccggccttgc cgccggctgc cagaccaccg acccctacac
     1021 cggcgagatg aagacgtcga gcgccacgtt cggcgctctc atcggcgcgg gcaccggcgc
     1081 cgcgatcggg atgatttccg gccacgacgc caagcagcgc aggcagcggg ccctcgtcgg
     1141 cgccggcgtc ggcgcgctcg ccggcggcgc cgtcggcggc tacatggaca agcaggaaag
     1201 cgagctgcgg gcgctgatgc gcgacagcgg cgtgacggtc acgcgcaaag gcgaggacat
     1261 cgtactcaac atgcccggca acgtcacgtt ccgcaccggc agcgcggact tgaacgcgca
     1321 gttcttcaag gtgctcgacg gcgtcgcgca ggtggcgagg aaatacgaca agaccatcat
     1381 cgagatcgcc ggtcacaccg acaacgtagg gggcacggat ttcaacaggc agctcgcgca
     1441 gcgccgcgcg agcaccgtcg cccggtacct cacgagcaaa ggcgtgagcg agccgcgcat
     1501 catgaccgca gccggcggcg aggatcaccc gatcgcgagc aactcgaccg agcagggccg
     1561 cgccgcgaac cggcgcgtcg aagtgacgct cgcgcccctt aaggaaggct gagccggccg
     1621 ggatctactc gtacatcccc gcgcgcgcct tgccgcggaa cacgcggtag gcgaaggcgg
     1681 tgtaggcgat gaggaaggga aacacgatcg cgacgcccac gagcatgaac ttcagcgccg
     1741 acgggtgcga agccgcctcc cagatagtca tgcgatcgat tacgacatag ggaaacaggc
     1801 tataggcgag gccggcgaag gcgagagtga agatcgccac cgtcgccgcc aggggcatcc
     1861 agtcgaattc gcgctcgccc cgcttcaagc ggccggtggc cgcccagacc cgccagcctg
     1921 cggcgagggt cgcaagagga agcagcatca aggccagcgt gcgcggaaaa tcgaaccact
     1981 tcgcctgcac cgtgtggctc acgagcggcg tggcaaggct caccagcgcg accccgagcg
     2041 cgacccacag cagaccccag cgcgcccagg cgagcgcctt tttctgcagc tcgccttcgg
     2101 tcttcaatat gagcca
//
LOCUS       DASPZD010000285         3851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_349464, whole genome shotgun sequence.
ACCESSION   DASPZD010000285 DASPZD010000000
VERSION     DASPZD010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349464"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VEM33_05565"
     CDS             <1..444
                     /locus_tag="VEM33_05565"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HYS51324.1"
                     /translation="LNYTRNVSRWVGPDDARRIAADVALSLETVIAETYKARGYEIAA
                     APEPGVLRFSASIADLYVNAPDRYSPWTVKTFTRDAGQATLVLEARDAVTGTLLARVV
                     HHGIAREISRINTATDVTNRFWFDTLFRRWTADCIAEFEAGRNRS"
     gene            complement(449..1618)
                     /locus_tag="VEM33_05570"
     CDS             complement(449..1618)
                     /locus_tag="VEM33_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HYS51325.1"
                     /translation="MHHPSSDARAHGIEALRKGDARKARESFERIVAAGQADASAYLG
                     LAYACAGLKDHPAARAAVDRALALEPRNLPALIMKADYLAALGDARAASSFYRAAVGI
                     APPADQLPADLRSELARAEAMCGRYAAQFSAYLRERLVARGLVERRSTGRFRDSLDIL
                     AGGKTIYFQEPRQYFFPGLPQIQFYDRDDFPWLDKVEAATADIRGELIEILKQESAFK
                     PYVEGNPNLPQTDPQGMLNNPEWSAFYLWKNGAIVPENAARCPKTLSALADVPLARVP
                     GRSPSILYSLLRPGARIPPHTGEVNTRLICHLPLVVPDKCSFRVGNDTRVTVEGRAWV
                     FDDTIEHEAWNGSDRTRVILLFEIWRPELTLEERALVSAMFEAIDAYGGEPAQGI"
     gene            complement(1632..2150)
                     /locus_tag="VEM33_05575"
     CDS             complement(1632..2150)
                     /locus_tag="VEM33_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51326.1"
                     /translation="MQIRVLAFISIFLVAGAAHADAAGDASAEIAKCAGIADSAARLK
                     CFDAAAPRANGAPAAQPAREAPDPDGFGFSPPQAVTRVEDFGKPPPLPEITRITATVI
                     ELARTARGRSIFVLANGQVWRQLDGDDTDVQDPQPGKTMKVAIETGLGLLGNYNLTIE
                     GRNGFVRVYRVK"
     gene            2427..>3851
                     /locus_tag="VEM33_05580"
     CDS             2427..>3851
                     /locus_tag="VEM33_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYS51327.1"
                     /translation="MSNSKRARTLFVKKSSVVATEFALALMAAQIAYAQQPPDPAAPP
                     QQPPEQAAPQQQPAAPAQTAAQPVRTAEQVERVEITGSRLPQLNVEGPSPVTVVNAQE
                     IRMDGLSKTEDILLALPQANATQTSVQSNGATGTANVNLRNLGPTRNLVLVNGRRLPA
                     GTPQSGAFSAAADLNQIPAPLIQRVEVLTGGASAVYGSDAITGVLNFIMNDRFEGLQV
                     DLYHSFYNHKQHNPSGVQDLINDRAVGNGAEFFKVPGNVGSDGAVNGISITMGNNFAD
                     NKGNATVFFGYKNEDAVSQANRDFSACSLDDGDSFACAGSSTTYPGRFRTNSGAGAQF
                     TIDPTVPGGIRPYAGAPDQFNFAPYNFFRRPSEQINFNTFVHLDIHPKVRTYMELNYH
                     DNKSDAAIAPGGIFYGDPFITIHGNNPLLSPAQRAIIAANNNGNPFAGANDTATLLIG
                     RRDVEGPVRNDLLRNTSSRYVLGAK"
BASE COUNT          702 a         1294 c         1207 g          648 t
ORIGIN      
        1 ctgaattaca cgcgcaatgt ctcccgctgg gtcgggccgg atgacgcccg gcgcatcgcc
       61 gcggacgtgg ccttgagcct cgagaccgtc atcgccgaga cgtacaaggc gcggggctac
      121 gaaatcgccg cggcgcctga accgggcgtc ctgcgctttt ccgcgagcat cgccgacctc
      181 tacgtcaacg cgccggaccg ctattctccg tggaccgtga aaaccttcac gcgggacgcc
      241 ggccaggcga cgctcgtgct cgaggcgcgc gacgcggtca ccggcacgct cctcgcgcgc
      301 gtggtacacc atggcatcgc acgcgagatc agccgcatca acacggcgac cgacgtcacg
      361 aaccgcttct ggttcgacac gctgttcagg cggtggacgg cggactgcat cgcggaattc
      421 gaggccggca ggaaccggtc ctgagctcct agattccctg cgccggctct ccgccgtaag
      481 cgtcgatcgc ctcgaacatc gcgctcacca gagcccgctc ctcgagggtg agctcggggc
      541 gccagatctc gaacaatagg atcactcgcg tccggtcgct gccgttccag gcttcgtgct
      601 cgatcgtgtc gtcgaacacc caggccctgc cttcgaccgt cacccgcgtg tcgttgccca
      661 cgcgaaagct gcacttgtcc ggcacgacga gcggcagatg gcagatcagg cgcgtattga
      721 cctcgcccgt gtgcggcgga atccgcgcgc cggggcgcag cagggagtag aggatcgaag
      781 gcgagcggcc cggcacgcgc gcgaggggga catcggcgag agcgctcagg gtcttcgggc
      841 agcgcgctgc gttctccggc acgatcgcgc cgttcttcca cagatagaac gcgctccact
      901 ccgggttgtt cagcatcccc tggggatccg tctggggcag gttcggattg ccctcgacat
      961 agggcttgaa cgccgactcc tgcttcagga tctcgatcag ctccccgcga atatccgccg
     1021 tcgcggcctc gaccttgtcg agccagggaa agtcgtccct gtcatagaac tggatctggg
     1081 ggagccccgg gaagaagtat tggcggggct cctggaagta gatcgtcttc ccgcccgcca
     1141 gaatgtcgag cgaatccctg aagcgcccgg tggagcgccg ctcgacgagc ccgcgggcga
     1201 cgagccgctc ccggagatag gctgagaatt gcgcggcgta gcgcccgcac atggcctctg
     1261 ccctcgcgag ctcgctgcgc aggtcggccg gcaattggtc ggcgggcgga gcgataccaa
     1321 ccgcagcgcg gtagaacgac gacgcagcgc gggcatcgcc caaagcggcg aggtaatcgg
     1381 ccttcataat gagcgcgggc agattgcgcg gctcgagcgc gagcgcgcga tcgaccgcgg
     1441 cgcgcgccgc cgggtggtcc ttgaggccgg cgcaggcata ggcgaggccg aggtaggcgg
     1501 aggcatccgc ctggccggcc gcgacgatcc gttcgaagga ctcgcgcgcc ttgcgggcgt
     1561 cgcctttgcg cagggcctcg atgccgtgcg cgcgcgcgtc gctggagggg tggtgcattc
     1621 cgatcgccgc cctacttcac ccggtaaacc ctgacaaagc cgttgcggcc ctcgatcgtg
     1681 aggttgtagt tgccgaggag accgagaccc gtctcgatcg cgactttcat cgttttgccc
     1741 ggctgcgggt cctggacatc ggtgtcgtcg ccgtccagct gcctccatac ctggccgttg
     1801 gcgagaacga agatggatcg gccgcgcgcg gtcctcgcca gctcgatcac cgtagccgtg
     1861 atccgggtga tctcgggcag gggcgggggt tttccgaaat cctccaccct ggtgacggcc
     1921 tggggcgggg aaaatccgaa cccgtccggg tcgggcgcct ccctggccgg ctgcgcggcg
     1981 ggcgcaccgt ttgcgcgcgg cgcggcggca tcgaagcact tcaagcgtgc ggccgagtcc
     2041 gcgatgcccg cgcattttgc gatctcggcc gacgcgtctc ccgcggcgtc ggcgtgcgcc
     2101 gcgccggcga cgaggaagat cgaaatgaaa gctagtacgc ggatttgcat ggaaaactca
     2161 cgcttcgcgc acctcctcgc cgggcgggca gtgcggcgca gcatcatcgg ttcgggaccc
     2221 ctttgtacac atattacatg ttggcatccg ccttgcatgc atggcaaggg ggaacggagg
     2281 acgcgtccgt cgtccgcggc cttgcctcga ccggaccttc caggcaactt gtaaaattgc
     2341 gcaacattcg ggtcacatgg agttacaatc cgcccgtcgg cggatctgtc attgccactt
     2401 ggacaacaaa ctcagggaga aaccaaatga gcaacagcaa acgcgcgcgg acgttgtttg
     2461 taaaaaagtc gtccgttgtc gccacggaat tcgcgctcgc tctgatggcg gcgcaaatcg
     2521 cttacgcgca gcaacctcct gatccggccg ctccaccgca gcaacctcct gagcaggccg
     2581 ctccacagca gcaacccgcc gcgccggcgc aaaccgccgc gcagcccgtg agaaccgccg
     2641 agcaggtcga gagagtcgaa atcaccggct cgcgcctgcc gcaactaaac gtcgaaggcc
     2701 ccagcccggt caccgtcgtg aacgcgcagg aaatccgcat ggatggcctg tccaagaccg
     2761 aggacattct tcttgccctg ccgcaggcca atgcgacgca gacctccgtt caatcgaacg
     2821 gcgccaccgg taccgcgaac gtcaacctgc gaaacctcgg cccgacccgt aacctcgtgc
     2881 tggtcaacgg gcgtcgcttg ccagctggga cgccgcaaag cggcgccttc agcgcggcag
     2941 ccgacttgaa ccagattccc gccccgctga tccagcgcgt cgaggtgctc accggcggcg
     3001 cctccgcagt gtatggctct gatgccatca ccggcgtctt gaacttcatc atgaacgacc
     3061 ggttcgaggg gctgcaggtc gatctctatc acagcttcta taaccacaag cagcacaacc
     3121 cgagcggcgt tcaggacctg atcaacgacc gggctgtggg gaatggcgcc gaattcttca
     3181 aagtgcctgg caatgtcggt tcggatggcg cagtgaacgg catcagcatc acgatgggca
     3241 acaacttcgc ggacaacaag ggcaacgcca cggtgttctt cgggtacaag aacgaggacg
     3301 ctgtttccca ggccaaccgg gatttcagcg cctgttccct ggatgacgga gatagcttcg
     3361 cgtgcgcggg gtcctcgacc acctacccgg ggcgcttccg cacgaattca ggcgccggag
     3421 cgcaatttac gatcgacccg actgttcccg gcggtatccg cccgtacgcc ggagctccgg
     3481 accagttcaa cttcgcccca tacaactttt tccgccgtcc atcggaacaa atcaatttca
     3541 atacgtttgt ccacctggac atccatccga aggttcgcac gtacatggag ctcaattacc
     3601 acgacaacaa gagcgacgcg gcaattgcgc cgggcggcat tttttacggc gatcccttta
     3661 taacgatcca cggcaataac ccccttcttt cccccgcgca gcgcgcaatc attgccgcga
     3721 acaacaatgg caacccgttc gccggtgcca acgatacggc aacgcttctc atcggccggc
     3781 gcgacgtaga agggccggtc cgcaacgact tgctgcgcaa tacctcgtcc cgctacgtgc
     3841 tgggtgcaaa g
//
LOCUS       DASPZD010000286         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_4393, whole genome shotgun sequence.
ACCESSION   DASPZD010000286 DASPZD010000000
VERSION     DASPZD010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4393"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1110)
                     /locus_tag="VEM33_05585"
     CDS             complement(76..1110)
                     /locus_tag="VEM33_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS51328.1"
                     /translation="MRWKGSIVLLQVLCVASACAQSPEKNQAVYLYEGSDRAQRLAER
                     ARQEGALMVYTSLATSESVPLTQAFEKKYGVKVELWRSLSDQVVRRALNEAKARRHVV
                     DVIETNAPELEALARERVLARFFSPHIADIPSWGVPAHRMWIADRVDFFVVAFNTARV
                     RREELPATYEGFLDPKWKGRIGLEATDQEWLAGLAKHWGERRAMEFFRGLVAMKPDVR
                     KGHVLLSEMVSAGEVHVSLTNYASNADSMKRRDKPIDWKPVEPVIGRPQAIGLAANAP
                     HPNAALLFADFVLSPEGQRLFDSMGRFPSSRKVKSDLIAFPYLMLDPIALIDEDEKWL
                     KLWNELVTVK"
     gene            1267..>2466
                     /locus_tag="VEM33_05590"
     CDS             1267..>2466
                     /locus_tag="VEM33_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS51329.1"
                     /translation="MPHEKEQTHWHEPAGTRKAGFGEFKKQPTPYDAFMESEGIPVFR
                     GIGIRKVQDLPLAPWKRTGGRGHFIQLTGTETKWGCYVVEIPPAGALNPEKHMYEEIF
                     LVVEGRGTTEVWLEGDAKRHVFEWQKGSMFSIPMNAWHRLVNATSGGALLLAGTTAPN
                     VLNMINNVDAVFNNPFVFRDRFSGADDFFKPRDDIEPDPVRGLAMRRTNFIPDIVNCD
                     LPLDNRRSPGWRRVEPFMTNNCFYFWIGQHENGRYSKGHAHTSAAVLICVKGKGYTYT
                     WPERLGVTPWKDGRMDEVKRVDYEPVGMVSAAPGGARWYHQHFSVSKDPFRLTAWFGP
                     HNPGRDPGAPGEKHTDYTAIDIPEGGTSIPYWMEDPFIRREYEATLVSNGAQNRMNAE
                     FYDKEYKG"
BASE COUNT          462 a          819 c          771 g          414 t
ORIGIN      
        1 atcccgtggc gcaggcccag gaggaacacc atcagcgcaa ggccggcgag gtcgtgcggg
       61 aggaccgaag tcatcctatt tgaccgtgac gagctcgttc cagagcttga gccatttttc
      121 gtcctcgtcg atcagggcga tggggtcgag catcaggtag ggaaaggcga tcaggtcgct
      181 tttcaccttt cggctggacg gaaaccgccc catggaatcg aaaagccgct gcccttccgg
      241 ggacagcacg aaatcggcga agagcagcgc cgcgttcggg tggggcgcgt tcgccgcgag
      301 cccgatcgcc tgcggcctgc cgatcaccgg ctcgaccggc ttccagtcga tcggcttgtc
      361 cctgcgcttc atcgagtcgg cgttggaggc gtagttggtg aggctgacgt gcacttctcc
      421 cgcggaaacc atctcggaga gcagcacgtg ccccttgcgg acatcgggct tcatcgcgac
      481 caggccgcga aagaactcca tggcgcggcg ctcgccccag tgcttggcga gccccgcgag
      541 ccactcctgg tcggtcgcct ccagcccgat ccgccctttc cacttgggat cgaggaaacc
      601 ttcataggtc gccgggagct cctcgcgacg aacccttgcc gtattgaacg ccacgacgaa
      661 gaaatcgacg cggtccgcga tccacatccg gtgcgcgggc acgccccagg aaggaatgtc
      721 cgcgatatgg gggctgaaga acctcgccaa tacccgctcg cgcgcgagcg cctccagctc
      781 cggggcgttg gtttcgatga catcgacgac atggcggcgc gccttggctt cgttcagggc
      841 gcgccgcacg acctggtcgc tgagcgaacg ccacagctcg actttgacgc cgtatttctt
      901 ttcgaacgcc tgggtgagcg gcaccgattc ggaggtggca agcgaggtat agaccatcag
      961 cgcgccttcc tgccgggccc gctccgccag gcgctgcgcg cggtcgctgc cctcgtagag
     1021 gtagaccgcc tgatttttct ccggggattg cgcgcaggca gacgcgacgc aaagcacctg
     1081 aagcaggacg atcgagccct tccaccgcat cggttcctcc ctccacgcgc ccgtgagatc
     1141 ctgtggcgct tttcgcccgc ggcgccgatt gacgctcgcg gggattatat ggtcgaatga
     1201 gcgcgctttg caaaactccg gaactgtgtt ctgtatatca gaacacggca tcaggcgagg
     1261 acccccatgc cgcatgagaa agagcagacc cactggcacg agccggccgg cacccgcaag
     1321 gccggcttcg gcgagttcaa gaagcagccg accccgtacg atgccttcat ggagtccgag
     1381 gggattcccg tcttccgcgg catcggcatc cgcaaggtgc aggatctgcc cctcgcaccc
     1441 tggaagcgca cgggcggccg cggccatttc atccagctca ccggcaccga gaccaagtgg
     1501 ggatgttacg tcgtcgagat cccccccgcg ggtgcgctga atcccgaaaa gcacatgtac
     1561 gaggagatct tcctcgtcgt cgagggacgc ggaacgaccg aggtgtggct cgaaggcgac
     1621 gcgaagaggc acgtgttcga gtggcagaaa gggtcgatgt tctcgatccc gatgaacgcc
     1681 tggcaccgtc tggtcaacgc cacttccggc ggcgcgctgc tgctcgcggg caccaccgcg
     1741 ccgaacgtgc tcaacatgat caacaacgtc gacgcggtgt tcaacaatcc cttcgtgttc
     1801 cgcgaccgct tcagcggcgc ggacgatttt ttcaagccca gggacgacat cgagcccgac
     1861 ccggtgcggg gcctcgcgat gcggcgcacg aatttcattc ccgatatcgt gaactgcgat
     1921 cttcccctgg acaacaggcg ctcgcccggc tggcggcgcg tcgagccttt catgaccaac
     1981 aactgcttct atttctggat cggccagcac gagaacggcc gctactcgaa gggacacgcg
     2041 cacacctccg ccgccgtgct gatctgcgtc aagggcaagg gctacaccta tacctggccc
     2101 gagcggctcg gcgtgacgcc gtggaaggac ggccgcatgg acgaagtgaa gcgcgtcgat
     2161 tacgagccgg tgggcatggt gtcggcggcc cccggcggcg cgcgctggta ccaccagcat
     2221 ttcagcgtct ccaaggaccc gttccgcctc accgcctggt tcggcccgca caaccccggc
     2281 cgcgaccccg gcgcgccggg cgagaaacac accgactaca ccgcgatcga cattcccgaa
     2341 ggcggaactt ccattcccta ctggatggag gacccgttca ttcgccgaga gtacgaagcc
     2401 accctggtga gcaacggggc gcagaaccgg atgaacgccg aattctacga caaggaatac
     2461 aaaggt
//
LOCUS       DASPZD010000287         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1359303, whole genome shotgun sequence.
ACCESSION   DASPZD010000287 DASPZD010000000
VERSION     DASPZD010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359303"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VEM33_05595"
     CDS             <1..932
                     /locus_tag="VEM33_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS51330.1"
                     /translation="EALAGGFVAVLEMPFKKRLLFNAIHSATAVQGSQEGVISLSDYY
                     AARDANRRRYQILVAEDNAVNQRVLVGILERAGHRVRVVDDGEQALDVLENERFDVII
                     MDLNMPELGGLDAARAYRFMDPGAIQVPIIMLSADVTSEAMKECEDAGIDAFLPKPVE
                     ARRLLDTIASLIAKRATAQAVSGDGAEESQAVINSAALSELELISSENTFMPELLNGF
                     IQDGEALLRQMDAAVAAGQYETLRDLVHAMKGSAVTLGAEQLCRTCVGINSQTTSELQ
                     ASGPRVLKMIREQFQQARASLLDYLKRSQSAAR"
     gene            970..1233
                     /locus_tag="VEM33_05600"
     CDS             970..1233
                     /locus_tag="VEM33_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYS51331.1"
                     /translation="MADLSLHDRIIQRLLAASDAKLTAADVGEATFLRQDLDISSLIL
                     ITLASELEDELEIEIDDEQLGQIQTIGDLFKAIEGKKRQNRQV"
     gene            1263..2498
                     /locus_tag="VEM33_05605"
     CDS             1263..2498
                     /locus_tag="VEM33_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HYS51332.1"
                     /translation="MAITGVGLVSCLGHDYSQVVKALQNGASGLRGMPDWESLGLKSR
                     VAGAITGVEAKFERSPIPKKLRPVISEGSKYCCLAALDAVGDSGLSEADVQSGRTACI
                     VGTGIGSVESIYTGGRQVHSGEIAKVSPFAVLKSMASASSAAVANLLKMHGRSYSISS
                     ACSTSSHSIGTAYELIRSGIVDRAIAGGGEEVHPLIAAGFQALRFALASKFNERPEQA
                     SRPYDVARDGFVIGGGAGIVVLEALDQALSRRARVRAEIVGFGTSSDGFDMVMPEPSG
                     VQAAQCMLSAIRDGGMSPREVGAVNTHGTATIKGDIAEVEAMKQVFDGKPPPFSSTKS
                     MTGHPLGAAGALETIFCVGMLEHRFIAPSINIEQLGPEFAGMPIVTAPKFTELHTILS
                     NSFGFGGTNASLLLKAFRN"
     gene            complement(2676..>3341)
                     /locus_tag="VEM33_05610"
     CDS             complement(2676..>3341)
                     /locus_tag="VEM33_05610"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HYS51333.1"
                     /translation="GNLANLVQLIKSGDHDTLMHYAKLGVDNMYQRICGYGAPITTIS
                     LVQGEALGGGFETALSSNVIVAERRSRMGLPEILFNLFPGNGAYSLLARRVGMKRAEE
                     MILSGRVYTAEEMHAAGVVDVLAEDGTGEAAVRDYIRRNERRRNGMQAVFSCRQHFHP
                     VSYEELLNIANLWVSAALRLEEKDLRLMSRLARSQAKLVPGGAAARPDAVEMREEMQY
                     ATA"
BASE COUNT          644 a         1042 c         1060 g          595 t
ORIGIN      
        1 ccgaagccct cgcgggcggt ttcgttgcgg tgctggagat gccgttcaag aagcggctgc
       61 tgttcaacgc cattcattcc gcgactgcgg ttcaaggcag ccaggaaggc gtcatctccc
      121 tcagcgatta ctacgccgcc cgcgacgcca accggcgccg ctaccagatc ctggtggccg
      181 aagacaatgc cgtcaatcag cgcgtcctcg tcgggatact cgagcgcgcc gggcacagag
      241 tccgggtcgt ggacgacggg gagcaggcgc tcgacgttct ggagaacgag cggttcgatg
      301 tgatcatcat ggacctcaat atgccggagc tcggcgggct ggacgctgcg cgggcatacc
      361 gcttcatgga cccgggggcg atacaggttc cgatcatcat gctttcggcc gacgtgacct
      421 cggaggcgat gaaggagtgc gaagacgccg ggatcgacgc tttcttgccc aagccggtcg
      481 aggctcgccg cctcctcgac accatcgcga gcctgattgc gaagcgcgcg accgctcagg
      541 cggtgtccgg cgatggcgcc gaggaaagcc aggcggtcat caattcggcg gcgctctcgg
      601 agctcgagct catcagttcc gagaacacct tcatgccgga actcctcaat ggattcatac
      661 aggacggcga ggcgctgctg cggcagatgg atgccgccgt ggcggccggg cagtacgaaa
      721 cgctcaggga cctggtgcac gccatgaagg gctcggcggt cacgctcggc gccgagcagt
      781 tatgccggac gtgcgtggga atcaacagcc agacgacatc ggaactgcaa gcaagcggac
      841 cccgcgtctt gaagatgata cgggagcagt ttcagcaggc gcgcgcttcg ctcctcgatt
      901 atctcaagag aagccagagc gcggcccggt gagccaggga cgtgacatga acaagcggcg
      961 tgagaaacca tggctgattt atcgctccat gaccgaataa tccagcgtct actggcggca
     1021 tccgatgcaa aacttaccgc agccgacgtc ggcgaagcga cattccttcg acaggacctg
     1081 gacatcagtt cactgattct gatcacactg gcttccgagc tggaggatga gttggagatc
     1141 gaaatcgacg atgagcaact tggccagata cagacgatcg gcgatctttt caaggcgatc
     1201 gagggcaaga agcgacagaa tcgtcaggtg tgacgcggtc tgagttgatg gctcctcggc
     1261 gcgtggcaat cacgggagtc ggtctcgtat cgtgccttgg gcacgattac agccaggtgg
     1321 taaaggctct ccaaaacggg gcaagcggcc ttcgcggcat gcccgactgg gaaagcctgg
     1381 gcctgaagag ccgtgtggcc ggtgcgatca cgggcgtcga ggcgaagttc gagcgctcgc
     1441 cgatcccgaa aaaactgcgc cccgtgattt cagaaggatc caagtactgt tgcctcgccg
     1501 cgctcgatgc cgtcggggac tccggccttt ccgaggccga cgtgcaatcc ggcagaacag
     1561 catgcattgt cgggaccgga atcggaagcg tggaatccat ctacaccggg gggcggcagg
     1621 tccattccgg agagatcgcg aaggtcagtc cgtttgccgt gctgaagagc atggcgagtg
     1681 cgtccagcgc ggcggtggcc aacctcctca agatgcacgg acgcagttat tcgatcagtt
     1741 ccgcgtgttc cacctcttcg catagcatcg gcacggcata cgagctcatc cgctccggca
     1801 tcgtggaccg agccatcgcg ggcggtggag aagaagtgca ccccttgatc gccgccggtt
     1861 tccaggccct tcgcttcgcg ctcgcctcga aattcaacga gcgccctgaa caggcgtcgc
     1921 gcccgtatga cgtagcccgc gacgggttcg tgatcggcgg cggtgcggga atcgtcgtgc
     1981 tcgaggcctt ggaccaggcg ctgtcgcgtc gcgcccgcgt gcgcgccgag atcgtgggat
     2041 tcggcacgag ctcggatggt ttcgacatgg tcatgcccga acccagcggc gtgcaggcgg
     2101 cccagtgcat gctgagcgcc atccgggacg gagggatgag ccctcgggaa gtcggcgccg
     2161 tcaacaccca cggcaccgcg accatcaagg gcgacatcgc agaagtcgag gcaatgaagc
     2221 aggtattcga cggcaaaccg ccgccattct catcgacgaa atcgatgacc ggccatcccc
     2281 ttggcgccgc gggggcgctc gaaacgatct tttgcgtagg catgctcgag catcgcttca
     2341 tagccccttc catcaacatc gagcaactcg gtcccgaatt cgcgggaatg ccgatcgtaa
     2401 cggctccgaa gttcacagag ctgcacacca tactttccaa cagcttcgga ttcggtggga
     2461 cgaacgcgag ccttctgctg aaggcatttc gcaactagcg cgacgggttt cattcgagcg
     2521 cctttccatc aggcgaggga acaagactcg ccgcgcatga actccaccgg ggcgcgtcct
     2581 tacgcaccgg tcgggacgga tcggatcacg gccccggcag gtcatggctc aagcgcgcga
     2641 gcgcccgtgc ttcgggcgga catgcacagc cgcgctcagg ctgtcgcgta ctgcatttcc
     2701 tcccgcattt ccaccgcgtc cggccgcgcc gccgcaccgc ccgggacgag ctttgcctgc
     2761 gagcgcgcca ggcggctcat cagcctgaga tccttctctt ccaggcgcag agccgcgctc
     2821 acccacaggt tggcgatgtt gagcaactcc tcatagctca cggggtggaa atgttggcgg
     2881 cagctgaaca cggcctgcat gccattgcgg cgccgctcgt tgcgccggat gtagtcccgc
     2941 acagccgctt ccccggtccc gtcttccgcg agcacatcga cgacgcccgc cgcgtgcatt
     3001 tcctcggcgg tgtagacacg gccggagagt atcatctctt cggcacgttt cattcccacg
     3061 cgtctggcga gcaggctgta ggcgccgttg ccaggaaaca gattgaacaa gatctcgggc
     3121 aggcccatcc gcgagcgtcg ctctgcgaca atcacgttcg aagagagcgc tgtttcgaat
     3181 ccgccgccaa gcgcctcccc ctgcaccagc gaaatggtgg ttatgggagc tccgtagccg
     3241 cagatccgct ggtacatgtt gtcgacgcca agcttggcat agtgcatgag cgtgtcgtgg
     3301 tcgccggact tgattagctg caccaaattc gcgaggtttc c
//
LOCUS       DASPZD010000288         3904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_316422, whole genome shotgun sequence.
ACCESSION   DASPZD010000288 DASPZD010000000
VERSION     DASPZD010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316422"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VEM33_05615"
     CDS             complement(<1..510)
                     /locus_tag="VEM33_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYS51334.1"
                     /translation="MSSADKANPYELDLDKNPANYASLTPLGFIERAAYVYPKRTAVI
                     HGTRRATWDETYARCRRLASALARRGIGVGDTVAAMLANTPEMLECHFGIPMAGAVIN
                     TLNTRLDADAIAFMLGHGGAKVLIADREFSGTVARALAVLEEKPFVIDVDDPEYSGAG
                     ERIGQVEYER"
     gene            complement(546..1274)
                     /gene="dnaQ"
                     /locus_tag="VEM33_05620"
     CDS             complement(546..1274)
                     /gene="dnaQ"
                     /locus_tag="VEM33_05620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HYS51335.1"
                     /translation="MRSVVLDTETTGLNAGLGDRIIELGCIEVLSRRVTGNHFHRYIN
                     PERESEEGALKVHGIDHEFLQDKPKFREIAAEFLDYIQGAELVIHNAAFDVEFLNLEL
                     GRLGLPPIEAHCAGIVDSLRIARELHPGKKNSLDALCERYQVDHAHRTLHGALLDAAL
                     LAEVYLAMTRGQDSLAIELDRPRPALERAAATDGERPPLIVFRPSAEELAEHARLLAD
                     IEAENKAQCVWRRFETTVQMLDRV"
     gene            complement(1344..1988)
                     /locus_tag="VEM33_05625"
     CDS             complement(1344..1988)
                     /locus_tag="VEM33_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYS51336.1"
                     /translation="MRRTKRESEHTRRSILAAARKVFARQGVTRTTLEEIAAAAGVTR
                     GAIYWHFADKNELFFAMREQVAVPMIDQIDLALLRSDGSDPLAGVERFLLGILEALEN
                     DASARQTFQIMGFKCEYVGEFERELALQRLRCSELIFKLTRAYRRAQRAGRLRIGLRP
                     PMAAVETCSFLMGLTRLWLLDAKRSLVRRVARGLISAHVTSHRRGGKERTLRNL"
     gene            2080..3282
                     /locus_tag="VEM33_05630"
     CDS             2080..3282
                     /locus_tag="VEM33_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYS51337.1"
                     /translation="MTSVRARPAFRGRHGLLHSSLPAIAGLLAATLLSGCEQGGANTP
                     ATNPAAAGIPARVLQVAPQRLAIVLEAVGQVQGSKEVEVRARVSGILLKRLYNEGDFV
                     REGSALFQIDPVPYQIALAQANAQMAQERARQEQTRREAARLKTLADEKAISQKEFDD
                     AISAQKLSDATLQAAQANLRQAELNVSYTKVTAPVSGVAGRMARSEGSLVTAGQESSL
                     LTTMNQVDPIWIRFSLSESDLAKLPDKRLDRIKSAEVRLALPDGSRYPLAGRLNFAAT
                     QIDPRLATQELRATFGNPGVRLLPGQFVRVQVAAGERDNVFLVPQTAVIQTEKTFLVF
                     TLDKENKAESRPVQVGDWVGADWTILGGLNPGDRVILDNLLKVRPGALVSPQAPAEAK
                     AAAAPASK"
     gene            3293..>3904
                     /locus_tag="VEM33_05635"
     CDS             3293..>3904
                     /locus_tag="VEM33_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYS51338.1"
                     /translation="MSRFFIERPIFASVIAIVITLAGLVAFQVLPVAQYPEIAPPTVT
                     ITAALPGASAETLSRTVAAPIEEQLSGVEGLLYFQSTSSSNGTLTITCTFEVGTDVNN
                     ATIQVNNRVQIALPRLPDDVKRTGVVVQKRSVDILLFVAVTSTDPRFDTLYLSNFMTI
                     NALDVLKRVPGVADATIFGARDYSMRIWLRPDKMAQHGVTATDV"
BASE COUNT          667 a         1323 c         1245 g          669 t
ORIGIN      
        1 gcgctcgtac tccacctggc cgatgcgctc gccagcgccc gagtactcgg gatcgtcaac
       61 gtcgatcacg aacggtttct cctccaggac ggcgagggct cgcgcgacgg tgcccgaaaa
      121 ctcgcggtcg gcgatcagga ccttggcccc gccgtgcccg agcatgaagg cgatcgcgtc
      181 cgcgtcgagg cgcgtgttca gcgtgttgat gaccgcgccc gccatgggaa tgccgaagtg
      241 gcactcgagc atttcagggg tattggcgag catcgccgca accgtgtcgc cgacgcctat
      301 cccgcggcgc gcaagcgcgg aggcgaggcg ccggcaacgc gcataggtct cgtcccacgt
      361 ggcgcggcgc gtcccgtgga tgaccgcggt gcgctttgga tagacgtaag cggcccgctc
      421 gataaatccc agcggcgtca gggacgcgta gttggccggg ttcttgtcga gatcgagctc
      481 gtagggattc gccttgtccg cgctcgacat cgccgttcga tcttgccgtg ctccgctgct
      541 cgttttcata cccgatcgag catctgcacc gtggtctcga agcggcgcca cacacattgc
      601 gccttgttct cggcctcgat gtcggcgagc aggcgagcgt gctcggcgag ctcttccgcg
      661 ctcggccgga agacaatgag cggaggccgc tcgccgtcgg tcgccgcggc tctctccaga
      721 gcggggcgcg gccggtcgag ctcgatcgca aggctgtcct gcccgcgggt catggccagg
      781 tagacctccg cgaggagcgc cgcgtcgagc agcgcgccgt ggagggtgcg gtgagcgtgg
      841 tcgacctgat agcgctcgca tagggcgtcg agggagtttt tcttcccggg gtgcagctcg
      901 cgcgcgatcc tcagggaatc gacgatcccc gcgcagtgcg cctcgatcgg cgggagcccg
      961 agcctgccca gctcgagatt caggaattcg acgtcgaagg ccgcgttgtg gatcaccagc
     1021 tcggcgccct ggatgtagtc caggaactcg gccgcgatct ctcggaactt gggtttgtcc
     1081 tggaggaact cgtggtcgat gccgtgcacc ttgagcgcgc cttcctcgct ctcgcgctcg
     1141 ggattgatgt agcggtgaaa gtggtttccc gtgacgcgcc ggctcaagac ctcgatgcag
     1201 cccagttcga tgatccggtc gccgagaccc gcgttgaggc ccgtggtttc ggtatccagg
     1261 acgacacttc gcatcggctc tagcctctcc acgccgcaac ccgcagctct gcccggtgag
     1321 agtagcgtcc ccgcgaaaat aaatcaaagg tttcgcagag tcctctcctt gccgccgcgc
     1381 cggtggctgg taacgtgagc ggagatcagg ccgcgagcga ccctgcgcac gagcgagcgc
     1441 ttggcgtcga gaagccacag ccgcgtcagg cccatcaaaa aactgcaggt ttcaacggca
     1501 gccatcggcg ggcgcagtcc aatccgcaac cgtccggcac gctgcgcgcg ccggtaggcg
     1561 cgcgtgagct tgaagatgag ctcggagcaa cgcaagcgct gaagcgccag ctcgcgctcg
     1621 aactcgccga cgtattcgca tttgaagccc atgatctgga atgtctggcg tgccgacgcg
     1681 tcgttttcga gcgcttccag aatgccgagg aggaagcgct cgacccccgc cagaggatcg
     1741 gacccgtcgg agcgcagcag cgcaagatcg atctgatcga tcatgggcac ggccacctgc
     1801 tcgcgcatgg cgaagaacag ctcgttcttg tcggcgaaat gccagtagat cgccccgcgc
     1861 gtgacgccgg ccgcggcggc gatctcctcc agcgtcgtgc gggtcacgcc ctggcgggcg
     1921 aagaccttgc gcgctgcggc taggatgctc cgccgggtgt gctcggattc ccgttttgtt
     1981 ctacgcaagt cggaaacgca gcatctttgg catacattcg tgattgtata ttatactcgc
     2041 gcgctcgaat cgaattctca aacaagagac gatctccgca tgacctcagt tcgcgctcgg
     2101 ccagccttcc gcggccgcca cggcctgttg cattcatcgc tgccggcgat tgccggattg
     2161 cttgcggcga ccttgctctc aggatgcgag caaggcgggg ccaatactcc cgccaccaat
     2221 cccgccgcgg ccggcatccc ggccagggtc ctccaggtgg cgccgcagcg cctggccatc
     2281 gttctcgaag ccgtcggcca ggtgcaaggc tccaaggaag tcgaagtgcg cgcgcgcgtt
     2341 tccggaatcc tgctcaagcg cctctacaac gaaggggatt tcgtgcgcga gggcagcgcc
     2401 ctcttccaga tcgacccggt cccctaccag atcgccctcg cgcaggccaa cgcgcagatg
     2461 gcgcaggaac gcgcacgcca ggaacagacg cgccgcgagg ccgcgcggct caagacactc
     2521 gccgacgaga aggcgattag ccagaaggaa ttcgacgatg cgatctcggc gcaaaagctg
     2581 tcggacgcga cgctccaggc agcccaggcg aatctgcgcc aggccgagct caacgtgtcc
     2641 tatacgaaag tgaccgcacc ggtctcggga gtcgccgggc gcatggcccg gtccgaaggc
     2701 agcctggtga ccgcaggaca ggagtcgagc ctgctcacga cgatgaatca ggtggatccg
     2761 atctggattc gcttcagcct ttcggaatcg gacctagcga agcttccaga caagaggctc
     2821 gatcgcatca aatccgcgga ggtgcgcctc gccctccccg acggctcgcg ctatccgctg
     2881 gctggccggc tcaatttcgc cgcgacccag atcgacccca ggctcgccac gcaagagtta
     2941 cgcgcgacgt tcggcaatcc gggtgtgcgc ctcttgcccg gccagttcgt gcgggtccag
     3001 gtggcggcgg gcgagcgcga caacgtattt ctcgtccccc agaccgcggt catccagacc
     3061 gagaaaacgt ttctggtgtt cacgctcgac aaggaaaaca aggccgagtc ccgcccggtc
     3121 caggtgggcg actgggtcgg cgccgactgg acgatcctcg gcggcctcaa tcccggcgac
     3181 cgggtcattc tcgacaacct gctcaaggtg cggccgggcg ccttggtgag tccccaggcc
     3241 ccggctgaag ccaaggccgc ggcagctcca gcctcaaagt aagcaaggcg ccatgtcgcg
     3301 attcttcatc gagcgaccga tcttcgcgtc ggtcatcgcg atcgtcatca cgctcgccgg
     3361 gctggttgcc ttccaagttt tgcccgtcgc gcaatacccg gagatcgcgc ccccgaccgt
     3421 tacgatcacc gccgcgcttc ccggcgcgag cgccgagacg ctctcgcgca ccgtcgccgc
     3481 gccgatcgag gagcagctct ccggggtcga gggtctgctc tacttccagt ccacctcctc
     3541 ctcgaacggc acgctgacca tcacctgcac tttcgaggtc gggaccgacg tcaacaacgc
     3601 cacgatccag gtgaacaacc gcgtgcagat cgcgctgccg cggctacccg acgacgtgaa
     3661 gcgcaccggc gtggtggtgc agaagcgctc ggtcgacatc ctcctcttcg tcgccgtgac
     3721 atcgaccgat ccgcgcttcg acacgctgta cctctcgaat ttcatgacga tcaacgcgct
     3781 cgacgtcctg aagcgcgtcc cgggcgtggc cgacgcgacc atcttcggcg cgcgcgacta
     3841 ctcgatgcgg atctggctgc gcccggacaa gatggcgcag cacggcgtca ccgccaccga
     3901 cgtc
//
LOCUS       DASPZD010000289         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1224212, whole genome shotgun sequence.
ACCESSION   DASPZD010000289 DASPZD010000000
VERSION     DASPZD010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224212"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEM33_05640"
     CDS             <1..249
                     /locus_tag="VEM33_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS51339.1"
                     /translation="SAGKPLYDPAEIRVPTFLAHAEWDQDLPSYMLYAYFAKLTNAPY
                     KRYVEIGEGTHTVIMEKNRMQLFQAVQQFLDEDLKPGR"
     gene            260..961
                     /locus_tag="VEM33_05645"
     CDS             260..961
                     /locus_tag="VEM33_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYS51340.1"
                     /translation="MKQPETALIVGAGSGLSASLARLFSRERMRVAVAARDTAKLASL
                     VKETSAFPIACDATDLSQIEAMFAAVEAKWGVPDLVVYNAGYRVRGPFIDLDPKEVER
                     TITVTGYAGFLVAQGAAKRMLPRGSGAIFFTGASASVKGYAESAPFAMGKFALRGLAQ
                     SMARELAPKGIHVAHFVIDGGIARGAGDPRAGERGADGMLHPDEIAKNYLHLYRQHRS
                     AWTWEIELRPWVEKF"
     gene            985..1410
                     /locus_tag="VEM33_05650"
     CDS             985..1410
                     /locus_tag="VEM33_05650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYS51341.1"
                     /translation="MPLRPGFDVERFNRFGKEFLPGFVGLLITEVSEGLVKGELELRK
                     ALLAPNGYLHAGAVVSFADTLAGYGCISHLPANAQNFTTVELKANFLNTAQEGRLHGE
                     ARAVHLGRTTQVWDALVTHGEERSKLALFRCTQLVLYPR"
     gene            1634..>2166
                     /locus_tag="VEM33_05655"
     CDS             1634..>2166
                     /locus_tag="VEM33_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51342.1"
                     /translation="MKTKFLGCSQVVAAFATVLTLGGGTAQAVTCNVPADRATIQLAV
                     NDVTCDPIKVAPGVYAENVMIPRSLTLNGAQAGFAVAGRVFGDPLTESTVTGVFATPL
                     PDITIQARGVTVDGFSLTNPGQSTGILIKTAGDSAFITNNIIEDIGGAAFSDNTQAIY
                     LENGPDNVSILNNDMKN"
BASE COUNT          435 a          671 c          685 g          375 t
ORIGIN      
        1 agcgcgggca agccgctcta cgacccggcg gagattcgcg ttccgacttt cctcgcgcat
       61 gccgaatggg accaggacct gccgagctac atgctctacg cctacttcgc caagcttacc
      121 aacgccccgt acaagcgcta cgtcgagatc ggcgagggca cgcacacggt catcatggag
      181 aagaaccgga tgcagctttt ccaggccgtg cagcagttcc tcgacgagga cttgaagccg
      241 ggccgctagc gcgcgagcca tgaagcagcc cgaaaccgcg ctcatcgtcg gcgcgggcag
      301 cggcctctcc gcatcgctcg cgcgcttgtt ctcgagagag cgcatgcggg tcgcggtggc
      361 cgcgcgtgac acggccaagc tcgcttcgct cgtgaaggaa acgagcgcgt tcccgatcgc
      421 ctgcgatgcc acggacctct cgcagatcga ggcgatgttt gccgcggtgg aagcgaaatg
      481 gggcgtgccc gacctcgtcg tctacaacgc gggatatcgc gtgcgcgggc ccttcatcga
      541 cctcgacccg aaggaagtcg agcgcacgat cacggtgacg ggctacgcgg ggtttctcgt
      601 cgctcaaggc gctgcgaagc gcatgctgcc tcgcggctcg ggagcgatct tcttcaccgg
      661 ggcgtcggcg agcgtgaagg gctacgccga atcggcgcct ttcgcgatgg ggaagttcgc
      721 gctgcgcggc ctcgcgcaga gcatggcgcg ggagctcgcg ccgaagggaa tccacgtcgc
      781 gcacttcgtc atcgacggcg gtatcgcgag gggcgcgggc gacccgcgag cgggcgaacg
      841 cggcgcggac ggtatgctcc accccgacga aatcgcgaag aactacctgc acctgtaccg
      901 ccagcaccgc agcgcctgga cctgggagat cgagctgcga ccctgggtcg agaaattcta
      961 gagcgagatc cgtaaggaga aaccatgccg cttcgtcccg gattcgacgt cgaacgcttc
     1021 aatcggttcg gcaaggagtt cctgcccggc ttcgtcgggc ttctcatcac cgaagtgtcc
     1081 gagggcctag tgaaagggga gctcgagctg cgcaaggcgc ttctcgcgcc gaacggatat
     1141 ctgcacgccg gggcggtcgt cagcttcgcc gacacgctcg cgggttacgg atgcatttcg
     1201 cacctgccgg cgaacgcgca gaacttcacc acggtggaac tcaaggcgaa tttcctcaat
     1261 acggcgcagg aaggcaggct ccacggcgaa gcgcgcgctg tgcacctggg gcgcaccacg
     1321 caggtctggg acgcgctcgt cacccacggc gaagagcgga gcaagctcgc gctgtttcgc
     1381 tgcacgcagc tggttcttta cccgaggtag cagaacgcat atcgcactgc ctgcgcgatt
     1441 gaagttgtag tcaggccgcg atgcagaatc gacgattctt tacggaaaaa tcggggttca
     1501 gcgacagaca gtctaatgcc ctccctccta tagtactacg cggatgagcc gaattggcca
     1561 tgtcacgtag gccaagttga ttacggcgca aacgtcgcaa gttcagtaga ccaaaaacaa
     1621 agggaggatc gacatgaaga cgaaattcct aggatgctcg caggttgtgg cggctttcgc
     1681 gaccgtgttg acgcttggcg gaggcacggc acaggcggtt acgtgcaacg tcccggcgga
     1741 tcgcgcaacg attcaacttg cagtcaacga tgtcacctgc gacccgatca aagttgctcc
     1801 gggggtgtac gccgagaacg tgatgatccc caggtctctc actctcaacg gcgcgcaggc
     1861 gggcttcgcc gtcgccgggc gagtgtttgg cgacccgctc accgaatcaa ccgtgaccgg
     1921 cgtgttcgct accccgttgc cggatatcac tatccaggcg cggggtgtga ccgtcgacgg
     1981 tttttcgctg accaatcccg gccaaagcac gggaattctg atcaagacgg ccggcgatag
     2041 tgccttcatc acgaacaaca tcatcgagga catcggcggc gcggccttta gcgacaatac
     2101 ccaggccatt tatctcgaaa acggtccgga taatgtgtcg atcctgaaca atgacatgaa
     2161 gaacat
//
LOCUS       DASPZD010000290         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_683626, whole genome shotgun sequence.
ACCESSION   DASPZD010000290 DASPZD010000000
VERSION     DASPZD010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683626"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VEM33_05660"
     CDS             complement(<1..811)
                     /locus_tag="VEM33_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51343.1"
                     /translation="MNIPISIAMASNPRTWPIFDGRVKPDGITLIPSSVHPSELFWRQ
                     LRFADFDVSEMSFSSLIMARSKGDDRWVGLPIFTTRKFFHAEILVRRDSGIDRPGDLK
                     GRRVGVPEYQQTAALWTRGVLEHEFGVAPKDMQFWMERPPSHSHRGAIGFSAPPGVTI
                     NQIPPEKNIGSMMLSGELDAAIHYIVNPNLVDRSTADLWNHPAIKPLFPDPLAEGVRY
                     YRKTGIFPINHGMVIRKEIVEKHPWIVLNLYKAFERANEIANRERLEHAEYH"
     gene            916..1890
                     /locus_tag="VEM33_05665"
     CDS             916..1890
                     /locus_tag="VEM33_05665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HYS51344.1"
                     /translation="MADLSNWAFPPEMQPNAEELRFDLEAALDSMVLLRSEVPEDAFT
                     AGILGTERIGNGVVIRDDGLILTIGYLITEATTIWLNTNKGAAVAGHPLAYDQATGFG
                     LVQPLGKLAAPALSRGTAASCRVGDDVVVAGHGGRKHALKAKLIARREFAGYWEYVLD
                     DALFTSPAHPHWSGAALIGAGGRLLGIGSLMVQEELGGRTVQGNMIVPIDLLEPILED
                     LVKLGRAQRPARPWLGLYATEVKGHVVVSGLASGGPAERAGVQVGDIVIEVANGRVSG
                     LADLFRKIWSTGAAGVEIPMTLVREGSVSRVRVPSADRQDLLKKPSLH"
BASE COUNT          384 a          626 c          682 g          386 t
ORIGIN      
        1 ggtgatactc ggcgtgctcc aggcgttcgc ggttcgcgat ttcattggcg cgctcgaacg
       61 ccttgtagag attcaggacg atccaggggt gcttttcaac gatctccttc cggataacca
      121 ttccatggtt gatgggaaag atgccggtct tgcggtagta acggacacct tcggccagcg
      181 gatcgggaaa gagcggcttg atcgcgggat gattccacag gtccgcggta ctccggtcga
      241 cgaggttggg attcacgatg taatggatcg ccgcatcgag ttctcccgag agcatcatcg
      301 atccgatgtt tttctccggc gggatctggt tgatcgtcac cccgggcggc gccgagaagc
      361 cgatcgcacc gcggtggctg tgcgaaggag ggcgctccat ccagaactgc atgtccttcg
      421 gcgcaacgcc gaactcgtgc tcgagcacgc cccgcgtcca gagcgcggcg gtctgctggt
      481 attcaggaac gccgacgcgc cgtcccttca ggtctccggg ccgatcgatc ccggaatccc
      541 ttcgcaccag gatctcggca tgaaaaaact ttctcgtcgt aaaaatcgga aggccgaccc
      601 agcggtcgtc gcccttcgac cttgccatga tcagcgagga aaacgacatc tcggagacgt
      661 cgaaatcggc aaagcgcagc tgccgccaga agagttccga cggatgcacg gagctcggta
      721 tcagcgtgat cccgtccggc ttcaccctgc cgtcgaaaat cggccaggtc cttggattgg
      781 aagccatcgc gatgctgatg gggatgttca tggccggtct cgactgcgag agtgtaccgg
      841 gaaattatcg cgcgggagct gacacgaagc cgggctctgc catagaatga cgggcgagat
      901 ttcgcaggaa atcccatggc ggacctgagc aactgggcgt ttccgcccga gatgcagccc
      961 aacgcagagg agttgcgctt cgacctggag gcggcgctcg actcgatggt gcttttgcgc
     1021 tccgaggttc cggaggacgc ctttaccgcc gggatcctcg gtaccgagcg catcggcaac
     1081 ggcgtggtca ttcgcgacga tggactcata ctgaccatcg gctatctgat caccgaagcg
     1141 acgaccatct ggctgaacac gaacaagggt gcggcggtag cgggtcatcc gctcgcctac
     1201 gatcaggcga ccggcttcgg attggtgcag cccctgggca agctggccgc gccggcgctc
     1261 tcgcggggca ctgcagcttc gtgccgcgtc ggcgacgatg tcgtcgtcgc cggtcacggc
     1321 ggccgcaagc atgcgctcaa ggccaagctt atcgccagga gggaatttgc aggttactgg
     1381 gagtacgtgc tggatgatgc gctctttacc agcccggcgc acccccactg gagcggcgcg
     1441 gcgttgatcg gcgcaggcgg gcggctcctc gggataggct cgctgatggt tcaggaagaa
     1501 ttgggcggcc gcacggtgca ggggaacatg atcgtaccga tcgacttgct cgagccgatc
     1561 ctcgaggacc tggtgaagct cggccgcgca cagcggcccg cccgcccctg gctcggcctt
     1621 tatgcgacgg aagtcaaagg acatgtcgtg gtttcgggac tcgcgagcgg cgggcccgca
     1681 gaacgcgccg gtgtgcaggt gggcgacatc gtgatcgagg tcgcgaacgg acgggtcagc
     1741 gggcttgccg acctgttccg gaaaatctgg agcaccggcg ccgcaggggt cgaaatcccg
     1801 atgaccctgg tgcgcgaggg ctccgtatcc cgcgttcgcg tgccctcggc cgaccgtcag
     1861 gatcttctca agaagccctc cttgcactga actttagcaa cctcgatcct tcggaccggt
     1921 ccaacgatgg atgcgaggtc aacccggcca ggctcgcgtg caggcaatgg agcatcgtgc
     1981 gatggagaac cgacagcatg atctgatgcg ccagcagatg atcgccgaga tcgtggccga
     2041 tgcggtattc cttacggctc agctcggcaa gtccgcgt
//
LOCUS       DASPZD010000291         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1080851, whole genome shotgun sequence.
ACCESSION   DASPZD010000291 DASPZD010000000
VERSION     DASPZD010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080851"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VEM33_05670"
     CDS             complement(<1..1134)
                     /locus_tag="VEM33_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYS51345.1"
                     /translation="MSGASESCSRNRLLALLAAFALAWFCNLGYRHLVKPDEGRYAEI
                     PREMVASGDWLTPRLNGFKYLEKPPLQYWATAVSFSVLGQNEWAARLWTALTGFLGVL
                     LVFSAGNRLFGPPAGLLGAAVTASAAIYVAIGHMLTLDMAVSFFMSATVFAFALAQRD
                     AASEGERRRWMLVAWATTALAVLSKGLIGIALPAGAVALYVLVERDWKLLKRLHAFSG
                     ALLFLAIAAPWFAAVSAANPEFLRFFFIHEHFERFLTREHDRYEPVWYFIPVVLIGVL
                     PWTVALFPALGRAWRRSPQARFQAARFLFIWCAVVFAFFSASSSKLGSYILPIFPALG
                     LLIGSALASAGPRLLLAQAGVAAGLGVALAVAASQATRFAASDL"
     gene            complement(1143..1514)
                     /locus_tag="VEM33_05675"
     CDS             complement(1143..1514)
                     /locus_tag="VEM33_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HYS51346.1"
                     /translation="MKLHLDPATAKNAITGYGDGYVMVNRQRFERSLVVLPDRILLDW
                     PPNRFDDLAPEHLAELAGLDREIILLGTGAQLRFPRPEIIQPLMRSGVGVEVMDMQAA
                     CRTYNILLAEDRRVAAALLIG"
     gene            1612..2802
                     /gene="alaC"
                     /locus_tag="VEM33_05680"
     CDS             1612..2802
                     /gene="alaC"
                     /locus_tag="VEM33_05680"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HYS51347.1"
                     /translation="MRKFARIERLPPYVFNITNELKMAARRAGEDVIDMSMGNPDGPT
                     PAHIVAKLVEAAQRSDTHGYSVSKGIPRLRRAICGWYQRRYGVAFDPDSEAIVTIGSK
                     EGLAHLMLATLDRGDTVLVPNPSYPIHIYGAIIAGADIRSVRMTAEADFIVELERAIR
                     DLIPKPKMLVIGFPSNPTAKCVELDFFERVIALARAHGIFVVHDLAYADITFDGWKAP
                     SIMQVKGARDIAVEFFTMSKSYNMAGWRIGFMVGNKDLVNALARIKSYHDYGTFTPIQ
                     VASIVALEGPQDCVEEHRMKYQKRRDVMVRGLHEAGWMVENPKASMYLWAKIPEAYAK
                     LGSLEFAKKILKDAKVAVSPGVGFGDYGDDHVRIALIENEARSRQAIRGIREMLRRDG
                     LIKP"
BASE COUNT          576 a          929 c          967 g          419 t
ORIGIN      
        1 aagatcggac gcggcaaatc gcgttgcctg cgacgcggcc accgccagcg cgacgccgag
       61 cccggccgcg acgccggcct gcgcgagcag gaggcgcggg ccggcggagg caagcgcgct
      121 cccgataagc agccccagtg ccgggaaaat cggcaggatg taggagccga gcttcgagct
      181 cgacgcggaa aagaaggcga aaacgacggc gcaccagata aagaggaacc gcgcggcctg
      241 gaatcgcgcc tgcggcgaac gcctccacgc gcgccccagc gcgggaaaga gcgcgacggt
      301 ccagggcagg acgccgatca gcacgacggg aatgaaatac cagacgggct cgtagcgatc
      361 gtgctcccgg gtaaggaagc gctcgaaatg ctcgtggatg aagaagaacc gcaggaactc
      421 cgggttggcc gccgaaaccg cggcgaacca cggagcggcg atcgccagga acaggagcgc
      481 gccggagaag gcgtgcagcc gcttgagcag cttccagtcg cgctcgacca ggacgtagag
      541 cgcgactgcg cccgcgggaa gggcgatgcc gatcaggccc ttgctcagca ccgcaagcgc
      601 ggtggtggcc caggcgacga gcatccagcg gcggcgttct ccttcgcttg ccgcgtcgcg
      661 ctgcgcgagc gcgaatgcaa acaccgtggc cgacatgaaa aagctgaccg ccatgtccag
      721 ggtgagcata tgaccgatcg cgacgtaaat cgcggcgctg gcggtgaccg cggccccgag
      781 caggcccgca ggcggtccga acaatcgatt cccggccgag aatacgagca ggacgcccag
      841 gaagccggtg agcgccgtcc acagccgcgc cgcccattcg ttctgtccga gcacgctgaa
      901 cgacacggcg gtcgcccagt actgcagcgg cggtttctcg aggtacttga aaccgttcag
      961 gcgcggggtg agccagtcgc cgctcgcgac catctcgcgc ggaatctccg cgtagcggcc
     1021 ttcgtcgggc ttgacgaggt gccgataacc gagattgcag aaccaagcaa gagcgaatgc
     1081 ggcaaggagc gcgaggagtc gattgcggga gcagctctcc gaagccccgg acattccgcc
     1141 gcctatccga tcagcagggc cgcggcgacc ctgcggtctt cggcgaggag aatattgtag
     1201 gtgcggcagg cggcctgcat gtccatgact tccacgccga ctccggaccg catcaggggc
     1261 tgaatgatct cgggacgcgg aaagcgcagc tgcgccccgg ttccgaggag gatgatttcc
     1321 cggtccaggc cggcgagctc cgccaggtgc tcgggcgcga ggtcgtcgaa tcggttcggc
     1381 ggccagtcga ggaggatgcg atccggcagc accaccaggc ttcgctcgaa cctctggcgg
     1441 ttgaccatca cgtaaccatc gccatagccg gtgatggcgt tcttggcggt cgcgggatcg
     1501 aggtgcagct tcaagtttgc ccgtgctgta agtgtccggt aacattataa ctttttgcag
     1561 cgcagggcgg ctcggaaagc ccgccgcact ccctggaaaa tcccgcgcga catgagaaag
     1621 tttgcccgaa tcgagcgtct tcctccctac gtcttcaaca tcaccaacga gttgaagatg
     1681 gcggcgcgcc gcgcgggcga ggacgtgatc gacatgagca tgggcaaccc cgacgggccc
     1741 acgcccgcgc acatcgtcgc gaagctcgtc gaggcggcac agcgcagcga cacgcacggc
     1801 tactcggttt cgaagggaat cccgcggctg cgccgcgcga tctgcggctg gtaccagcgc
     1861 cgctacggag tcgcgttcga tccggattcg gaagcgatcg tcaccatcgg atcgaaggag
     1921 ggtctcgcgc acctgatgct cgccacgctc gaccgcggcg acacggtgct cgtccccaac
     1981 ccgagctacc cgatccacat ctacggcgcg atcatcgccg gcgccgacat ccgctcggtg
     2041 cgcatgactg cggaggcgga tttcatcgtc gagctcgagc gcgccatccg cgacctgatt
     2101 cccaagccca agatgctggt gatcgggttt ccgtcgaacc cgaccgcgaa atgcgtggag
     2161 ctggacttct tcgagcgcgt gatcgcgctc gccagggcgc acggcatctt cgtcgtccac
     2221 gacctcgcct acgccgacat cactttcgac gggtggaagg cgccttcgat catgcaggtg
     2281 aagggcgcgc gcgacatcgc agtggaattc ttcaccatgt cgaagagcta caacatggcg
     2341 ggctggcgaa tcggcttcat ggtcggcaac aaggacctcg tcaacgcgct cgcgcgcatc
     2401 aagagctatc acgattacgg caccttcacg ccgatccagg tcgcctcgat cgtggcgctc
     2461 gaaggcccgc aggactgcgt cgaggagcac cgcatgaaat accagaagcg ccgcgacgtc
     2521 atggtgcgcg gcctgcacga agccggctgg atggtcgaga acccgaaagc ctcgatgtat
     2581 ctctgggcga agattcccga agcctacgcg aagctcggct cgctcgaatt cgccaagaag
     2641 atattgaagg acgctaaggt cgcggtgtcg ccgggggtcg gcttcggcga ttacggcgac
     2701 gaccacgtgc gcatcgcgct gatcgagaac gaggcccggt cgcgccaggc gatccgcggc
     2761 atcagggaaa tgctccgcag ggacgggctg atcaagccat gaacatcgtc cacatcaccg
     2821 acgacgcggg cagggtcgtc gagcccgcat ggttgaagag ggcggaaagc gtgcaccgcc
     2881 agctccggac g
//
LOCUS       DASPZD010000292         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_638012, whole genome shotgun sequence.
ACCESSION   DASPZD010000292 DASPZD010000000
VERSION     DASPZD010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638012"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VEM33_05685"
     CDS             <1..613
                     /locus_tag="VEM33_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51348.1"
                     /translation="GANGAGKSSTLRALSGLAPAAPGSIRFDGRDLAGVPAFARARAG
                     LVMVPEGRGVFAQLSVEENLAMGAYARSDAGVASDRARVFELFPRLAERCRQTAGTLS
                     GGEQQMLAIGRALMSRPKLLALDEPSMGLAPIAAQKILEVIRDINSTGVTVLLVEQNA
                     RSALALAHRAYVMESGEITLSGEARALLADPRVREAYLGEAAG"
     gene            complement(621..1646)
                     /locus_tag="VEM33_05690"
     CDS             complement(621..1646)
                     /locus_tag="VEM33_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HYS51349.1"
                     /translation="MTHQVTIRNTGHRFPAQDGSTILQSALDAGLVLPYGCRDGACGS
                     CKGKLVDGRVDYGRYSEKALTAQERAQGYALFCQAKPLSDVAIEAREVRKAGDILVRK
                     LPARVQKLERAADDAMIVYLKLPANERLMFLPGQYIDILLKDGSRRSFSMANAPHDDE
                     FVQLHVRHVPGGAFTDHVFQTMKERDILRLEGPFGTFFLRDESAKPIVLVASGTGFAP
                     IKALIEAAFKKGVKRPMRLYWGARRPKDLYLNGLAERWAGGHPGFEYVPVISDALPED
                     KWSGRIGFVHRAVMEDLPDLSGHQVYACGVPIMVDSARRDFIQTCRLPENEFFADSFT
                     TAADKAH"
     gene            1671..>2057
                     /locus_tag="VEM33_05695"
     CDS             1671..>2057
                     /locus_tag="VEM33_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYS51350.1"
                     /translation="MENLLIIGCGDVARRALPELQRRYRVTALVRSLDPVLDSAGVGQ
                     IAGDLDDAASLAPLLGRAERIAHLAPPSAGGTLDTRMRSLLAALAPRGRGAMLPRRFV
                     YLSSSGVYGDCAGEWIDETRPPRPQTG"
BASE COUNT          322 a          683 c          705 g          347 t
ORIGIN      
        1 cggcgcgaac ggcgcgggca agtcctcgac gctgcgcgcc ctctccgggc ttgcgccggc
       61 agctccgggg tcgatccggt tcgacggccg tgatctcgcc ggagtccccg cgtttgcgcg
      121 cgcgcgggcc gggctcgtca tggtgcccga gggacgcggg gtgttcgcgc aactgagcgt
      181 cgaggaaaat ctcgcgatgg gagcttacgc ccggtccgac gccggggtcg cctccgaccg
      241 cgcgcgcgtt ttcgagctgt ttccgcggct cgcggagcgg tgccgccaga ccgccggcac
      301 gctgtccgga ggcgagcaac agatgctcgc catcggccgc gccctgatga gccggccgaa
      361 gctcctcgcg ctggatgaac cgagcatggg actcgccccg atcgcggcgc agaaaatcct
      421 ggaggtgatc cgcgacatca acagcaccgg cgtgacggtg ctgctggtgg agcagaacgc
      481 ccgaagcgcg cttgcgctgg cgcaccgcgc ctatgtcatg gaaagcggag agatcacgct
      541 ctcgggagaa gcgcgcgcgc tgctcgccga cccgcgcgtg cgcgaagcct atctcggcga
      601 ggcggcgggg tagtccggcg ctagtgcgcc ttgtcggcgg cggtggtgaa ggaatcggcg
      661 aagaattcgt tttccggcag ccggcacgtt tgtatgaaat cgcgccgtgc ggaatccacc
      721 atgatcggca cgccgcaagc ataaacctgg tggccggaaa ggtcgggcag gtcctccatc
      781 accgcgcggt gcacgaaccc aattcggccg ctccacttgt cttccggcag cgcatcggaa
      841 atcaccggca cgtactcaaa ccccggatgt ccgccggccc agcgctcggc caaaccgttc
      901 aggtaaagat ccttcgggcg gcgagcgccc cagtaaagcc tcatggggcg cttgacgcct
      961 tttttgaacg cggcttcgat cagcgccttg atcggcgcaa atccggtgcc cgaggcgacg
     1021 agcacgatgg gcttcgcgga ttcgtcgcgc aggaagaagg tgccgaacgg tccttccaag
     1081 cgcaggatat cgcgctcctt catggtctgg aacacgtggt cggtgaatgc gccgcccggc
     1141 acatggcgca cgtgcagctg aacgaactcg tcgtcgtgcg gcgcgttggc catggaaaag
     1201 cttcgccgcg agccgtcctt caggaggatg tcgatgtact ggccgggaag gaacatcagg
     1261 cgctcgttcg cggggagctt cagatagacg atcatggcgt cgtccgcggc gcgctccagc
     1321 ttctgcacgc gcgcgggaag tttcctcacg aggatatcgc ccgctttgcg cacttcgcgg
     1381 gcttcgatcg ccacgtccga caggggcttc gcctggcaga agagcgcgta accctgcgcg
     1441 cgctcctgcg cggtgagcgc cttttccgag taacggccgt agtcgaccct gccatcgacg
     1501 agcttcccct tgcagctgcc gcaggcgccg tcgcggcagc cgtaagggag cacgagacct
     1561 gcatcgagcg ccgactgcag gatcgtcgag ccgtcctgcg ccggaaaccg gtggccggtg
     1621 ttgcgtatcg tgacctgatg cgtcatcggt gcgcggggta gaatccgttc gtggaaaatc
     1681 tgctcatcat cggttgcggt gacgtcgcgc gccgcgcttt gccggagctg cagcgtcgct
     1741 atcgtgtgac ggctctggtg cgctcgctcg atccggtgct cgattccgcc ggggtcgggc
     1801 aaatcgcggg cgatctcgac gatgccgcct cgctcgcgcc gctactgggc cgcgccgaac
     1861 gcatcgcgca tctcgcgccg ccctccgcag gcggaacgct cgatactcgt atgcgcagcc
     1921 tgcttgcagc cctcgcgccg cgcggacgcg gcgccatgtt accacggcga ttcgtgtacc
     1981 tctcctcgag cggggtctat ggcgactgtg cgggcgaatg gatcgacgag acgcgcccgc
     2041 ccaggccgca gaccggg
//
LOCUS       DASPZD010000293         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_124464, whole genome shotgun sequence.
ACCESSION   DASPZD010000293 DASPZD010000000
VERSION     DASPZD010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124464"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VEM33_05700"
     CDS             <1..480
                     /locus_tag="VEM33_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYS51351.1"
                     /translation="VVRQESRFIIGAKSSAGATGLMQVMRPTAKWVAQRMRMKNFSSA
                     RLHEPDLNAALGTYYLKHVLNQFDGSPVLAAAAYNAGPTRARLWRGTAPVEGAVFVET
                     IPFGETRDYVKKVMTNTVYYAAVLGTEPTPLKARLGTVLPRRSSEGVAVIQNPPVVQ"
     gene            563..1513
                     /locus_tag="VEM33_05705"
     CDS             563..1513
                     /locus_tag="VEM33_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HYS51352.1"
                     /translation="MSRILVIGGSGFVGRHLVRQLVEDGHFVVVPTRRRERAKHLILL
                     PTVDVVEADVHHPETLAKLTAGCDAVVNLVGILHGRPGRPYGPEFARVHVELPKKIVE
                     ACNKKRVPRLLHMSALHAHADGPSQYLRSKGEGEAWVLAAQDTQAVTVFRPSVIFGPE
                     DQFLNTFAGLQRWLPLVLLPSPQVRFQPVYVEDVARCFSASLADRDSIGKRYDLCGPR
                     AYTLRELVKYAGIASGHPRPVIGLSDRLSFLSAWAMEWKPGRKLMSRDNYNSMKVDSV
                     SSAAFPFSIQPTPLEAVAPVYLRGIFPRSRYSWFRYKAGR"
     gene            1526..2185
                     /locus_tag="VEM33_05710"
     CDS             1526..2185
                     /locus_tag="VEM33_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYS51353.1"
                     /translation="MATRILVIGNRNYSSWSLRPWLFLKQNAIAFEEVRIPLYQASSK
                     ERLLRYSPSGKVPVLVEGDIRIWDSLAILEYLAETHPSARGWPADVVARATARSVCAE
                     MHSGFPALRQNLSMNVRKVFARRTWPAEVSEEIERIQSVWQSCRERYGTGGPFLFGHF
                     SAADAMYAPVVWRFIGYSVPLAPAARAYCDAMLALPPMQEWRAAAGTEAETLSQFEMA
                     P"
     gene            2199..>2807
                     /gene="cca"
                     /locus_tag="VEM33_05715"
     CDS             2199..>2807
                     /gene="cca"
                     /locus_tag="VEM33_05715"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052569.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HYS51354.1"
                     /translation="MKIYAVGGAVRDELLGLPVADRDHVVVGSTPEEMVRLGYKPVGK
                     DFPVFLHPRTHEEHALARTERKVARGYKGFRIHAAPDVTLEQDLARRDLTINAMARDE
                     AGRLIDPFGGAADLERGLLRHVSPAFVEDPVRILRVARFAARFGFAVAPETMALMRSM
                     VANGETDALVPERVWQEFSKGLMEGDAALMFPVLAEAGLLEKL"
BASE COUNT          450 a          951 c          916 g          490 t
ORIGIN      
        1 gtcgtgcgcc aggaaagccg tttcatcatc ggtgcaaaat cctccgccgg cgcgaccggc
       61 ctcatgcagg tgatgcgtcc gaccgcgaag tgggtggcgc agagaatgcg catgaagaac
      121 ttctcctcgg cgcggctgca cgaaccggac ctcaatgccg ctctcggcac gtattacctc
      181 aagcacgtcc tcaatcagtt cgatggcagc ccggttctgg cagcggccgc gtacaacgcc
      241 gggcccacgc gcgcgcgcct gtggcgcggc acagctccgg tcgaaggcgc ggtcttcgtc
      301 gagaccattc ccttcggcga gacgcgcgac tacgtgaaga aggtgatgac gaacacggtt
      361 tactacgccg ccgtcctcgg caccgagcca acccccttga aggcgcgcct gggcacggtc
      421 ctgcctcgcc gctcctccga gggcgtggct gtaattcaga acccgcccgt cgttcaatag
      481 ggttcgcggg accatcgaag cggtcgggcg cacctgcccg gcgcgcttcg aggttacact
      541 agcgctttcc cttggcagca gcatgagtcg cattcttgtc atcggcggca gcggctttgt
      601 cggccggcat ctcgtgcgcc agctcgtaga agacgggcac ttcgtcgtcg tgcccacgcg
      661 ccgacgcgag cgtgccaagc acctgattct cctgccgacg gtcgatgtgg tcgaggccga
      721 cgtgcatcac ccggagaccc tcgcgaagct caccgccggc tgcgacgcgg tcgtcaatct
      781 cgtcggcatc ctgcatggac gcccgggccg cccctatgga ccggagttcg cacgcgtgca
      841 cgtcgagctc ccgaaaaaaa tcgtcgaggc ttgcaacaag aaacgcgtgc cgcgcctgct
      901 gcacatgagc gcgctgcatg cccacgccga cggccccagc caatacctgc gttccaaggg
      961 cgaaggcgag gcctgggtgc tcgcggccca ggacacgcag gcggtgacgg tgttccggcc
     1021 ttcggtgata ttcgggcccg aggatcagtt tctcaacacc ttcgccgggc tccagcgctg
     1081 gctgccgctc gtgctccttc ccagcccgca ggtacgcttc cagcccgtct acgtcgagga
     1141 cgtggcgcgc tgtttcagcg cgagcctcgc cgaccgcgac agcatcggca agcgctatga
     1201 cctgtgcggg ccgcgtgctt ataccctgcg agagctcgtg aaatacgccg gtatcgcgag
     1261 cggccacccg cggcccgtca tcggcctgtc cgaccgtctt tccttcctca gcgcctgggc
     1321 catggaatgg aagccgggca ggaagctcat gtcgcgcgac aactacaatt cgatgaaggt
     1381 ggacagcgtt tcgagcgctg ccttcccctt ttccatccag cccacgcctc tcgaggcggt
     1441 cgcgcccgtc tacctccgcg gcatttttcc gcgctcgcgc tacagctggt tccgctacaa
     1501 ggcgggccgc taggcttccc tgatcatggc gacccggatc ctggtcatcg gcaacaggaa
     1561 ctactcctcc tggtctctgc ggccgtggct gttcctgaag cagaacgcaa tcgcgttcga
     1621 ggaagttcgc atccccctgt accaggcaag ctcgaaggag cgcctgctga ggtactcgcc
     1681 atccgggaag gttccagtgc tggtcgaggg ggacatccgc atctgggatt cgcttgcgat
     1741 cctcgaatat ctcgcggaaa cacatccgag cgcccggggg tggcccgccg acgtggtcgc
     1801 gcgggctacg gcgcggtccg tgtgcgccga aatgcactcg ggctttcccg cgctgcgcca
     1861 gaacctgtcg atgaacgtgc gcaaggtttt cgcgcggcgt acctggcccg cggaggtcag
     1921 cgaggagatc gaacgcatcc aatcggtctg gcagagctgc cgcgagcgct acggaacggg
     1981 cggcccgttc cttttcggtc atttctctgc agccgatgcc atgtacgcgc cggtcgtgtg
     2041 gcgcttcatc ggctactcgg tgcccctcgc gccggcggcg cgcgcctact gtgacgcaat
     2101 gctcgccttg ccgccgatgc aggaatggcg cgcggccgcg ggaaccgagg ccgaaacgct
     2161 gtcgcagttc gagatggcgc cctgaaaggc aggtcgcggt gaagatttac gccgtcggcg
     2221 gagcagtgcg cgacgagctc ctcgggctgc ccgtggcaga ccgcgaccac gtcgtggtcg
     2281 ggagcacgcc ggaggaaatg gtgcgactcg gctacaagcc cgtgggcaag gatttcccgg
     2341 tgttcctgca tccgcgaact cacgaggagc acgctctcgc gcgcaccgag cgcaaggtcg
     2401 cgcgcggcta caagggcttc cggatccacg ccgcgcccga cgtcacgctc gagcaggacc
     2461 tcgcgcggcg agacctgacc atcaacgcga tggcgcgcga cgaagccggc cggctcatcg
     2521 atccattcgg cggcgcggcc gatctcgaac gcggactgct ccggcacgtg agccccgcgt
     2581 tcgtcgagga cccggtgcgg atcctgcgag ttgcgcgctt tgctgcccgc ttcggttttg
     2641 cggtcgcgcc ggagaccatg gcgttgatgc gctcgatggt cgcaaacggc gagacggacg
     2701 cgctcgtgcc ggagcgcgtg tggcaggagt tctccaaggg cctgatggag ggtgatgcgg
     2761 ctctgatgtt cccggtgctc gccgaggcgg gactcctcga gaagctg
//
LOCUS       DASPZD010000294         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1448344, whole genome shotgun sequence.
ACCESSION   DASPZD010000294 DASPZD010000000
VERSION     DASPZD010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448344"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VEM33_05720"
     CDS             complement(<1..238)
                     /locus_tag="VEM33_05720"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HYS51355.1"
                     /translation="MNLKQLWTAALGELQVGLSRAQYDTWFKDTQVLSEEDDVFLIGV
                     PNAFAREWLESKFRPQVRAALQHLLGRTVDVRFVT"
     gene            459..596
                     /gene="rpmH"
                     /locus_tag="VEM33_05725"
     CDS             459..596
                     /gene="rpmH"
                     /locus_tag="VEM33_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYS51356.1"
                     /translation="MKRTYQPKTRRRARVHGFRSRMRTTGGRRVLAARRARGRKRLTV
                     V"
     gene            610..957
                     /gene="rnpA"
                     /locus_tag="VEM33_05730"
     CDS             610..957
                     /gene="rnpA"
                     /locus_tag="VEM33_05730"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYS51357.1"
                     /translation="MDAARLRRDADIAAVWSEGMKIQHSMFALRARPNGLDEIRVAVA
                     APRLLGRAVARNRSRRRLREAFRAAIGDLVSGPGYDVVIVARPLTAPAAFADLRKAAA
                     VALGSIAERVAAT"
     gene            954..1178
                     /gene="yidD"
                     /locus_tag="VEM33_05735"
     CDS             954..1178
                     /gene="yidD"
                     /locus_tag="VEM33_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYS51358.1"
                     /translation="MKTLVLGALRFYKAAVSPMLPPACRYMPTCSEYAMEAVERYGVI
                     RGGWLATKRLFSCHPWSRGGYDPVPERRAG"
     gene            1178..>2038
                     /locus_tag="VEM33_05740"
     CDS             1178..>2038
                     /locus_tag="VEM33_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HYS51359.1"
                     /translation="MPPIVTEIWNALLVHPLVNLLVLADYFVHDFGLAVVIVTVAIRL
                     ALFPVFRAQVRSSRAMQELAPAMNDIKKKHGNDRAKLQQEQMKLYKERNINPIGGCLP
                     MVVFFPVLFAMYAAFQQVGGLSGAHALTVEQLRDSVLWPFLQFTVADLPPDRTIDLTA
                     HWLPWVSNLAQPDLAFFGLIGPLPIISSLLQLVASIQALPRNPVQTDDPTQRTMQSMT
                     YYLPLITIVFFRTLASGVFIYYITTTIFQIIQQYFVMGWGQLPRFIPPLANIPTPADK
                     TMRAREQAAIA"
BASE COUNT          346 a          699 c          611 g          382 t
ORIGIN      
        1 aggtcacgaa gcgcacgtcg acggttcggc cgagaaggtg ctgaagtgcg gcacgtacct
       61 gaggtcggaa tttgctctcg agccactcgc gcgcgaacgc attcggcaca ccgatgagga
      121 aaacgtcgtc ctcttcggag aggacctgcg tgtccttgaa ccaggtgtcg tactgcgcgc
      181 gcgaaagtcc gacctggagc tcaccgaggg cggccgtcca aagctgcttg aggttcaacg
      241 gctccccttc tcttcgctcg tgcaatagac gctccccaca acactcatgc aataaccgcc
      301 caacgccaca ccgaagggaa acaccgatgt tccgaccgcc cgctctcgga agcccgcgaa
      361 ctatacccca aggggaagac gccccgtcta ctgctatgct catgtgcaca caccgtcgtt
      421 tttgatcgct ttgtttcaac gggagaaggg actcgacctt gaagcgcacc tatcagccga
      481 agacccgccg acgtgcgcgt gtgcatggat tccgatcgcg catgaggacc accggcggcc
      541 gtcgagtgct tgccgcgcgc cgcgcgcgcg gccgcaagcg cctcaccgtc gtctagcgcc
      601 gcgtcattcg tggacgccgc gcgtctgcgg cgggatgcgg acatcgccgc ggtgtggagc
      661 gaaggaatga agattcagca ttcgatgttc gcccttcgcg ctcgccccaa tggccttgat
      721 gagattcgcg tcgcggtcgc cgcaccgcga ttgcttggcc gcgcggtcgc tcgcaatcgt
      781 tcgcgtcgcc gactgcgtga agccttccgc gcggcgatcg gcgacctggt gagcggaccc
      841 ggctacgacg tcgtgatcgt ggcgcgaccc ttgaccgccc ccgccgcatt cgccgatctt
      901 cgcaaggccg cggcggtggc gcttggatcg atcgcggagc gcgtggcggc cacgtgaaga
      961 cgctcgttct cggcgccctt cgcttctaca aggccgcagt gagcccgatg ctgccgcctg
     1021 cctgccgtta catgcccacg tgctcggaat acgcgatgga agcggtcgag cggtatggcg
     1081 tcatccgtgg cggctggctc gcgacgaagc gcctcttctc gtgccatccc tggagccggg
     1141 gcggatacga cccggtgcct gagcggcggg cgggctgatg ccgccgatcg tcacagagat
     1201 atggaacgcc ctcctcgttc acccgctggt caacctgctc gtcctggccg actacttcgt
     1261 tcacgacttc gggctggctg tcgtgatcgt caccgtcgcg atccgcctcg cgctcttccc
     1321 cgtcttccgc gcgcaggtcc gctcctcacg cgcgatgcag gagctcgccc ccgcgatgaa
     1381 cgacatcaag aagaagcacg gcaacgaccg cgcgaagctc cagcaggagc agatgaagct
     1441 ctacaaggaa cggaacatca atcccatcgg aggctgcctt ccgatggtcg tctttttccc
     1501 ggtgctgttc gccatgtacg cggcgttcca gcaggtcggt ggtctcagcg gggcgcatgc
     1561 gctcacggtc gagcagctga gggacagcgt cctctggcca ttcctgcagt tcacggtcgc
     1621 cgacctaccg cctgatagga cgatcgacct caccgctcat tggctcccgt gggtctcgaa
     1681 cctcgcgcaa ccggatcttg ccttcttcgg gctcatcggc cccttgccga tcatctcttc
     1741 gctgttgcag ctggtcgcgt cgatccaggc gcttccgcgc aatccggtgc agaccgacga
     1801 cccgacgcag cgcacgatgc aatcgatgac ctactacctg ccgttgatca cgatcgtctt
     1861 cttcaggact ctggcatctg gagtcttcat ctactacatc acgaccacga tcttccagat
     1921 catccagcag tacttcgtca tgggatgggg ccagctgccg cgctttatcc cgccgctcgc
     1981 gaacataccg acacccgccg acaagacgat gcgggcgcgc gagcaggcgg cgatcgcc
//
LOCUS       DASPZD010000295         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1327564, whole genome shotgun sequence.
ACCESSION   DASPZD010000295 DASPZD010000000
VERSION     DASPZD010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327564"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VEM33_05745"
     CDS             complement(<1..666)
                     /locus_tag="VEM33_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51360.1"
                     /translation="MLKLICAIALAGAAAAVSAQDYPSHPVKLVVPFPAGAGPDQVAR
                     ILSQQLHESLGQPFIVENRSGALGALGAAEVARSAPDGYTILITTNTTQAANVALFKN
                     LQYDPAKDFAPIIRLITTSMMLMVRSDFPAQNLRAFVAHARSTPGGLTAGYGSAGTQV
                     SIAKLKSLGKFSTVDVPYKGVPLAVADTLSGHVSFTFADFAVGFAQIKGGKMKSLGVT
                     SLVR"
     gene            995..2047
                     /locus_tag="VEM33_05750"
     CDS             995..2047
                     /locus_tag="VEM33_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS51361.1"
                     /translation="MSRPAASLLIFVLASFVVPGTYARDRQAANREIYLYQGADRAQR
                     LLSGARKEGGLSLYTTMTPEDAGPLVAAFEERYGIKVRMWRGINQKLVQRALAEARAG
                     KSAVDVFEGSGHGMEILHREALLEKFWSPAFGDIPPEAFPRHAHYAPDNLLFFVMGYN
                     TRLVKPEDVPRSYEDLLQPRWSGNFGIEASDIIWFAAVAKAMGEEKGLAYFRKLAAME
                     PQIRSGHTLMTELVAAGDIPMALTLYNQAVDKLKERGAPIDWKPLPPAFGRADGIGVA
                     KQAPHPHAALLFADFVLSPEGQRFIMAASRVPVNRKVESSFDQRNFRIIELAPVVDAW
                     DTWERRWQALFLKGQK"
     gene            2050..2859
                     /locus_tag="VEM33_05755"
     CDS             2050..2859
                     /locus_tag="VEM33_05755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYS51362.1"
                     /translation="MAPDAQKLRFGGDCEGFLYLPRSESPAGAVVILHERYGLVKHTL
                     DLAQRLARDGYVALAPNLFSRWRGDEAALRSGKTRVVLPDAEVAAVIDAALDELKRHP
                     RVAPGRIALMGVCQSGRYPIVIGSRRRDLAALVVFYGGSQERDWQLSDLQPQPMQEMM
                     RSLSAPVLFVYGERDHTISLEDVRRVRDALESGRRSYRMKLFAGMPHGWLNDTMPGRY
                     RAKEAKEAWVLLTRFLADVFSGTWPGADEVRWEFESATSVDYDFSKNTRLE"
BASE COUNT          507 a          954 c         1035 g          502 t
ORIGIN      
        1 gcgtacgagc gaagtcacgc cgaggctttt catcttgccg cctttgatct gcgcgaagcc
       61 gaccgcgaag tccgcgaagg tgaacgagac atggcccgac agcgtgtcgg ctacggcgag
      121 cggtacaccc ttgtagggca catccacggt ggagaatttg ccgagggact tgagcttggc
      181 gatcgacacc tgcgtgccgg cggacccgta gcccgcagtc agcccgccgg gggtgctccg
      241 cgcatgggcg acgaatgcgc gaaggttctg cgccggaaaa tccgagcgca ccatcagcat
      301 catcgaggtc gtgatcaggc ggatgatcgg agcgaagtcc ttcgccggat cgtactggag
      361 gttcttgaac agcgcgacgt tggcggcctg cgtggtattc gtggtgatga ggatcgtgta
      421 accgtcgggc gcgctgcgcg ccacttcggc ggcgcccagg gcgccgagcg cgcccgagcg
      481 gttctcgacg atgaaaggct gccctaggga ctcatgcagc tgctgactca ggatgcgggc
      541 gacctggtcg ggcccggccc cggcggggaa gggcacgacg agcttcaccg gatggctggg
      601 gtaatcctgc gccgataccg ccgccgcggc gcctgcaaga gcgatcgcgc agatgagctt
      661 caacatgccg cctccgggaa aactcgcgaa ggcagtgtat ccggacgctt cggcatttcg
      721 caagctgcgc tgctgctcga agtccttgcg ccgtggacaa gccaatcgag gcgtatattc
      781 cggttctgct cccccccttg atcggaagaa aggtcgccat gctccgctgc cgcttcagca
      841 tctttgcatt gctggtggtc ccgctctgcg ttcttgctca accgaaagca ccctactcat
      901 cgtggcggtc gtgcgctgga tcgagcgcag ggcaatcgac gaattcgtga agcggctgct
      961 cgccgccgtt aggccctcgg gcaagtaact tcggatgtct cgccccgctg ccagcctgct
     1021 gatctttgtc ctcgcctcgt ttgtggtgcc ggggacgtac gcgcgggacc ggcaggcggc
     1081 caaccgagaa atctatctct atcagggcgc ggaccgcgcg cagaggctgc tctccggggc
     1141 gaggaaagaa ggcggcctca gcctctacac caccatgacg ccggaagacg cgggaccgct
     1201 cgtcgcggcg ttcgaggaaa gatacggcat caaggtgagg atgtggcgcg gcatcaacca
     1261 gaagctcgtg cagcgcgcgc tcgccgaggc gcgcgcgggc aaatcggcgg tcgacgtctt
     1321 cgagggcagc gggcacggca tggagatcct gcaccgcgaa gcgctgctcg agaagttctg
     1381 gagccccgcg ttcggcgaca ttccaccgga ggctttcccg cgccacgccc attacgcgcc
     1441 ggacaatctg ctcttcttcg tcatgggcta caacaccagg ctcgtcaagc cggaggacgt
     1501 tcccaggtcc tatgaagacc tgctgcagcc caggtggagc ggcaatttcg ggatcgaggc
     1561 gtccgacatc atctggttcg cggcggtggc gaaggcgatg ggcgaggaaa aaggcctcgc
     1621 ctatttccgg aaactggcgg cgatggagcc gcagatccgc agcggccaca cgcttatgac
     1681 cgagctggtg gccgcaggcg acattccgat ggcattgacg ctctacaatc aggcggtgga
     1741 caagctgaag gagcggggcg cgccgatcga ctggaagccg ctgccgcccg cgttcggccg
     1801 ggccgacggc atcggcgtgg cgaagcaggc gccgcacccg cacgcggcgc tgctcttcgc
     1861 ggatttcgtg ctgtcgcccg aagggcagcg cttcatcatg gccgctagcc gcgtgccggt
     1921 gaatcgcaag gtcgagagct cgttcgacca gcgcaatttc cgcatcatcg agctggcgcc
     1981 ggtcgtcgac gcgtgggaca cctgggaacg gcgctggcag gcgctcttcc tcaaggggca
     2041 gaaataggca tggccccgga cgcgcaaaag ctgcgattcg gcggggactg cgagggcttt
     2101 ctctatctgc cgcgaagcga atcgccggcg ggcgccgtcg tcatcctgca cgagcgctac
     2161 ggtctggtaa agcacacgct cgacctggcg cagcgcctcg cgcgcgacgg ttacgtggct
     2221 ctcgcgccga atctcttctc gcgctggagg ggcgacgagg ccgcgctgcg gagcggcaag
     2281 acgcgcgtgg tcctgcccga cgccgaggtc gcggcggtga tcgacgcggc gctcgatgag
     2341 ctcaagcgcc atccacgcgt cgcgcccggg cgcatcgcgc tcatgggcgt gtgccagagc
     2401 gggcgctatc cgatcgtgat cggcagccgg cggcgcgatc tcgcggcctt ggtcgtgttc
     2461 tacggcgggt cgcaggagcg cgattggcag ctgtccgatc tgcagccgca gccgatgcag
     2521 gagatgatgc gctcgctttc ggcgccggtg ctcttcgtct acggcgagcg cgatcacacg
     2581 atctcgctcg aggacgtgcg ccgcgtgcgc gacgcgctcg agtcgggccg ccgcagctac
     2641 cgcatgaagc tcttcgccgg catgccgcac ggctggctga acgacaccat gccggggcgc
     2701 taccgcgcga aagaggcgaa ggaagcctgg gtcctgctga cccgatttct cgccgacgtc
     2761 ttctcgggca cctggccggg cgcagacgag gtacgctggg aattcgagag cgcaacctcc
     2821 gtcgattacg atttttcgaa aaacacgagg ctggaatgat cgagcgcctg gacccggcgc
     2881 gcaccgcgct ccttttcttc gacttgctga acgggcacgt gaagaagagc gatgcggcga
     2941 cgaagtcgcg ctacgcgccg gtgatcgccg cggccgtgcg ggttctggac gcggcgcg
//
LOCUS       DASPZD010000296         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_732945, whole genome shotgun sequence.
ACCESSION   DASPZD010000296 DASPZD010000000
VERSION     DASPZD010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732945"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VEM33_05760"
     CDS             <1..808
                     /locus_tag="VEM33_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS51363.1"
                     /translation="RDFSMAGRGFAIGARYYRPLGTKREEALPAVVYFHGGGWTCGDL
                     DTHDSVCRGIAAHGYCAVVAVDYRMGPEHRFPAAVEDALTSLQWVAANASSLGVDPGR
                     LVVAGESAGGNLAAVAALALRDSALHLAMQVLVYPVTDQAADTDSLRRFAQGYLLTRE
                     LLRWYQGNYLRDERDRIDWRASPLRAQDHSRLPPAYVITAGFDPLQDEGKAYAERLVQ
                     AGVDVTHECFEGQVHGFLPMAGVIAAAHHAHYRIGQMLRMRFGALPIVRP"
     gene            835..>2785
                     /locus_tag="VEM33_05765"
     CDS             835..>2785
                     /locus_tag="VEM33_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYS51364.1"
                     /translation="MLAPDKKVSVERKVVRAACPHDCPDTCAMKITVENGVAVKVEGA
                     KDHPFTSGTLCTKVARYLERTYSKDRVLFPQKRIGPKGRGEFERISWDEAIDTIAEKF
                     RAVEAEDPQGILPYDYAGTMGYVQWHSMGRRFFNRLGASRLDRTICSSAGKAGMLITL
                     GGSFGMDPERFDEAKLILIWGSNPIVSNLHLWSRVQEAKRRGAKLVAIDPYRSLSAEK
                     CHEHLALLPGTDAALALGMMHVIIGENRCDADYVHKYTIGFEALARRVEEYPPRKVAE
                     ITGLPAEKIIALAREYAATRPAAIRLNYGLQRHAGGGMAVRNILCLPALTGAWRDPAG
                     GAVLSTGGFFGMNDAKLERPDLLELSRCPNPRTINMVAIGDALLGAKPAIRALYVYNS
                     NPVAVAPDSAKVVRGFSREDLFTVVHEQFLTDTCDYADIVLPATTQLEHHDVHTAYGH
                     LYVVANSPAIAPLGESLPNSEAFRRLAARMGFAEECFKDTDEDICRQALESSDPRMQG
                     MEWDSLKEKGWRRLNVPERFAPFAQGNFGTPSGKCEFYSETAKRLGMDPLPTYIPPRE
                     SAQSNAALAGRYPLAIISPLNRHFLNSSFANMPFAIAEAKEPGLEIHPRDAAARGIAN
                     GDAVRIFNDRGSLTARARVSDKAREG"
BASE COUNT          480 a          945 c          917 g          443 t
ORIGIN      
        1 gcgcgatttc tccatggccg gtcggggatt cgcgatcggc gcccggtact accggccgct
       61 gggcaccaag cgcgaagagg cgctgcccgc agtggtgtac ttccacggcg gcggctggac
      121 ctgcggggat ctcgatacac acgacagcgt ctgccgcggg attgccgctc acggttactg
      181 cgccgtcgtc gcggtcgatt accgcatggg accggagcac aggtttccgg ccgcggtcga
      241 ggatgccctg acctcgttgc agtgggtggc cgcgaacgca agttccctcg gggtcgaccc
      301 ggggcggctc gtggtcgccg gcgaaagcgc cggcggcaac ctcgcggcgg tcgcggcgct
      361 cgccttgcgc gattcggcgc tgcacctcgc catgcaggtg ctcgtctatc ccgtgaccga
      421 ccaggccgcc gacacggatt cgctgaggag gttcgcgcag ggctatttgc tgacgcgcga
      481 attgctgcgc tggtatcaag gcaactatct gcgcgacgag cgcgaccgca tcgactggcg
      541 cgcatctccc ttgcgcgctc aagaccattc gcgcctgccg cccgcctatg taatcaccgc
      601 cggattcgat ccgctgcagg acgaaggcaa agcctacgcc gagcgcctcg tgcaagccgg
      661 cgtcgacgtg acccatgagt gcttcgaagg ccaggtgcac gggtttctcc ccatggcggg
      721 ggtgatcgcc gcggcacacc acgcccatta ccgcatcggc cagatgctgc gcatgcgctt
      781 tggagcgctt ccgatcgtgc gcccctgatc ggcgtaaaat cgctgaatcc tcatatgctg
      841 gccccggaca agaaagtttc cgtcgagcgc aaggtggtgc gcgccgcctg cccgcacgac
      901 tgccccgaca cctgcgccat gaagattacc gtcgagaacg gcgtcgcggt gaaagtggag
      961 ggcgcgaaag accatccctt cacctcgggc accctctgca cgaaagtggc gcggtatctg
     1021 gagcgcacct acagcaagga ccgggtgctc tttccgcaga agagaatcgg tccgaagggc
     1081 aggggcgagt tcgagcgcat ttcctgggac gaggcgatcg acaccatcgc cgaaaagttc
     1141 cgcgcggtcg aggccgagga tccacagggc atcctgccct acgactacgc gggcaccatg
     1201 ggctacgtgc agtggcactc gatgggcagg cgcttcttca acaggctggg cgcctcgcgc
     1261 ctggaccgca ccatctgctc ctcggcgggc aaggcgggga tgctgatcac gctgggcggc
     1321 tcgttcggca tggacccgga gaggttcgac gaggcgaagc ttatcctcat ttggggctcg
     1381 aatcccattg tctcgaacct tcatctgtgg tcgcgcgtgc aggaggcgaa gcgtcgcggc
     1441 gcgaagctcg tcgcgatcga cccctaccgc agcctttcgg ccgagaaatg ccacgagcac
     1501 ctcgccctgc tgcccggcac cgacgccgcg ctcgcgctcg ggatgatgca cgtcattatc
     1561 ggcgagaacc gttgcgacgc ggactacgtg cacaaatata cgatcggttt cgaagcgctc
     1621 gcccggcgcg tggaggaata cccgccgcgc aaagtcgcgg agatcaccgg cctgcccgcc
     1681 gaaaaaataa tcgcgctcgc ccgggaatac gccgcgacgc gtcccgcggc gatccgtctc
     1741 aactacgggc tgcagcgcca cgcgggcggg ggcatggccg tgcgcaacat cctctgcctt
     1801 cccgcgctca cgggtgcgtg gcgcgatccc gcgggcgggg cggtgctgag cacaggcggc
     1861 ttcttcggca tgaacgacgc gaagctcgag cggcccgacc tgctcgagct ctccaggtgc
     1921 ccgaacccgc gaacgatcaa catggtggcg atcggggacg cgctgctcgg agcgaaaccg
     1981 gcgatccgcg cgctctacgt gtacaactcc aacccggtcg cggtcgcgcc cgattcggcg
     2041 aaggtggtga ggggtttttc ccgggaggac ctcttcaccg tggtgcacga gcaattcctc
     2101 accgacacct gcgactacgc cgacatcgtc ctgccggcga ccacgcagct cgaacaccac
     2161 gacgtgcaca cggcctacgg gcacctgtac gtcgtcgcga acagcccggc gatcgcgccg
     2221 ctgggcgagt ccctcccgaa cagcgaggcg ttcaggcgtc tggccgcgcg catgggtttc
     2281 gccgaggagt gcttcaagga caccgacgag gacatctgcc ggcaggcgct cgaatcgtcg
     2341 gacccgcgca tgcaggggat ggagtgggat tcgctcaagg agaagggctg gcggcgattg
     2401 aacgtgcccg agcgcttcgc tccgttcgcg cagggcaact tcggaacgcc ttcggggaag
     2461 tgcgagttct acagcgaaac cgcgaagcgc ctgggcatgg atccgcttcc gacttacatc
     2521 cctccgcgcg agtcggcgca gtcgaacgcg gcgctcgcgg gccggtaccc gctcgcgatc
     2581 atctcgccgc tgaaccggca cttcctcaat tcgtccttcg ccaacatgcc cttcgcgatc
     2641 gccgaagcca aagagcccgg cctcgaaatc catcccaggg acgctgccgc ccgggggata
     2701 gcgaacggcg acgcggtgcg catcttcaac gaccgcggct ccctcaccgc cagggcgcgc
     2761 gtcagcgaca aggcgcgcga aggag
//
LOCUS       DASPZD010000297         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1381752, whole genome shotgun sequence.
ACCESSION   DASPZD010000297 DASPZD010000000
VERSION     DASPZD010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381752"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VEM33_05770"
     CDS             complement(<1..1229)
                     /locus_tag="VEM33_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51365.1"
                     /translation="MAARWRRGRSRHVRSSQVRFNIGARRDDATPLNDRAGALVRVMA
                     ITDFRSLRRFTGVNVIAASPALRVAAIAAIHAAALLAVCLTEYGPFATTLALLTWAFL
                     NFFWLILLRRPAVAAALSLVMIVSMIVLSRFKYDILEMTLSFFDVLVVDADTVAFLLT
                     IFPDLRPALAAAAIVAVPLLILIWRLDPFQLRRRTSSFGAAVSLAGMAGLASMVPEEP
                     WEPFSGVNHISNFARSGVVAVSELMGHGFFEADAAAADRLKPLSGETCRPAGKPPHII
                     MVLDESSFDITAAPGIKVPPGYADHFRSFDGKARSLLVEATGGPSWYSEYNVLTGLSA
                     RSYGRFMFTVTRIAAGRVERGLPQALRRCGYRTFSLYPAYGAFLGARRFQTGTGVEHF
                     SDLKEMGATSDMQPDRF"
     gene            1429..1851
                     /locus_tag="VEM33_05775"
     CDS             1429..1851
                     /locus_tag="VEM33_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51366.1"
                     /translation="MIRKKAAKKKAPPRRAARTKAKSTAKVAVKPTPVSQRVQPEAVK
                     PPPSRPAQPTAAEQRIGVVTHYYSHLSVVAMQLEPGTTLRVGDVIHIRGHTTDFTQKV
                     ESLEVNHAAVTEVGPNDDFGLKVVEHAREHDIVYRVRS"
     gene            1951..>3179
                     /locus_tag="VEM33_05780"
     CDS             1951..>3179
                     /locus_tag="VEM33_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYS51367.1"
                     /translation="MLIGGKWVDSASGEVLEVEDPAHRRPIAEVPRGGAEDVARAVQA
                     AADAFPAWSRTIPRERGRLLARIADALEARLEELARTIALETGNALRTQARPEAKTTV
                     EIIRYFGGLGSELKGETIPFGEHVLSYTRREPLGVVGAIIPWNAPVLLAALKIGPALC
                     AGNTLVLKSAEDAPLGVLMLAEICQEFLPPGVLNVLTGLGEECGAPLANHPMVSKLSF
                     TGSTEVGKLIMRAASDRIVPVSLELGGKSPSIVFPDANEDWVIDGIIAAMRFTRQSQS
                     CTAGSRLFLHRDIFDNFLSALGQKVKALKLGDPLDETSDIGTIINEKQFRKVCGYVEE
                     GLKRREARLVIGGLPPKEGPLSEGYYAIPTIFANVANDWHLAREEIFGPVLVAIPWTD
                     EADAIRMANDSHYGLAA"
BASE COUNT          656 a          995 c         1034 g          494 t
ORIGIN      
        1 tagaaccggt cgggctgcat gtcgctcgtc gcgcccatct ccttcaggtc gctgaagtgc
       61 tcgacgccgg tgccggtctg gaacctgcgg gcgccgagga aggcgccgta ggccggatag
      121 aggctgaacg tccggtagcc gcagcggcgc agcgcctgcg gcaggccgcg ctcgacgcgc
      181 ccggccgcga tgcgcgtgac agtgaacatg aagcgcccgt aggagcgcgc cgaaagaccg
      241 gtcagcacgt tgtactcgga gtaccagctc ggcccgccgg tcgcctcgac gagcaaggat
      301 cgcgccttgc cgtcgaagga gcggaagtga tcggcatagc cgggcggaac cttgatgccg
      361 ggcgccgccg tgatgtcgaa gctcgattcg tcgagcacca tgatgatgtg cggcggcttg
      421 ccggccggac ggcaggtctc gcccgaaagg ggtttcaggc ggtccgcggc cgcggcgtcg
      481 gcttcgaaga agccgtggcc catcagctcg gacactgcca ccacaccgga gcgggcgaag
      541 ttggaaatgt ggttgacgcc ggagaacggc tcccagggct cttccggcac catgctggcg
      601 agccccgcca tgccggcaag cgagacggcc gcgccgaagc tcgacgtgcg tcgccgcagc
      661 tggaacggat cgagccgcca gatcaggatg agcagcggaa ccgcgacgat cgcggccgcc
      721 gccagcgccg ggcgcagatc gggaaatatg gtcaacagaa acgcgaccgt atcggcgtcg
      781 acgacgagca cgtcgaagaa gctcaaggtc atttccagaa tgtcgtactt gaagcgggag
      841 agcacgatca tgctcacgat catcaccagc gacagcgccg ccgccacggc cgggcggcga
      901 agcaggatga gccagaaaaa attgaggaag gcccaggtca gcagcgccag cgtcgtcgca
      961 aacggaccgt attcggtcag gcagacggcg agcagcgccg ccgcgtggat ggccgcgatg
     1021 gctgcgaccc ggagcgcagg ggatgccgcg atgacgttca cgcccgtaaa gcggcgaaga
     1081 gaacggaaat cggtgatcgc catgacccgt acgagcgcgc cggcccgatc gttcagcggg
     1141 gttgcatcgt cccggcgcgc accaatgtta aaccttactt ggctgctgcg cacgtgccgc
     1201 gagcggccac gccgccaccg cgccgccaag ccgcgccgcc aagcagtata acagccttct
     1261 ggcctgttga cttaccttct tattcggcaa cgattggacg aagactttcc aaatgcagtg
     1321 agcgtcaggg aggctgccat ggcgacaaaa actagagcca aggcgaagcg caagaaaacg
     1381 accgtcacca agacggcaag gcgcagaaag acggttagaa aaactaggat gatcagaaag
     1441 aaagcggcga agaagaaggc gccgccccgg cgagcggcac gcaccaaagc caagtcgacc
     1501 gcaaaggtcg cggtcaagcc gacgccagtc agccaacggg tacagcctga agcagtcaaa
     1561 ccgccgccga gccggcctgc acagcctact gcggcggagc agcgcatcgg cgtcgtgacc
     1621 cactattaca gccacctctc agtcgtggcc atgcagctcg agcccggtac tacgctgcgc
     1681 gttggcgatg taatccacat ccgcggacac accaccgatt tcacccagaa ggtcgagtcc
     1741 ctcgaggtta atcatgcggc agtgaccgag gtagggccaa acgacgattt cggactaaag
     1801 gtggtcgagc atgcgcgtga gcacgacatc gtgtacagag tgcgatcgta gcgccgctgg
     1861 ccggaccaag ctgccgaagg aacgacggag gcgatcatga cggctgttac gaaaattcgc
     1921 actgacgagg tcgcgtttag gaaagcgcgc atgttgatcg gtgggaagtg ggttgatagc
     1981 gcttcaggcg aggtgctcga ggtcgaggat ccggcgcatc ggcgcccgat cgccgaggtc
     2041 ccccgagggg gcgccgagga cgtcgcgcgc gcagtgcaag ccgcggccga tgcctttccc
     2101 gcctggtcgc gcacgatccc tcgcgagcgc gggcgccttc tcgcgcggat tgccgatgcg
     2161 ctcgaggcgc ggctcgagga gctggcgcgc acgattgcgc ttgagaccgg gaacgcgctt
     2221 cgcacccagg cacgccctga ggcaaaaacc acagtcgaga tcatccgcta cttcggcggc
     2281 ttgggcagcg aactcaaggg cgaaacgatc cccttcggcg agcatgttct gagctacacg
     2341 cggcgtgagc cgctcggcgt tgtcggcgcc atcatcccgt ggaacgcgcc agtcctactg
     2401 gctgctctga agatcgggcc cgcgctttgc gccggcaaca cgctcgtctt gaagtccgca
     2461 gaggatgcac cgctcggcgt cctgatgctc gctgaaatat gtcaggaatt cttgccgcct
     2521 ggcgtgctca acgtgttgac tgggctgggc gaggaatgtg gcgctccgct cgccaaccac
     2581 ccgatggtaa gcaagctctc cttcaccggc tcgacggagg tcggcaagct catcatgcgc
     2641 gctgcctcag accgcatcgt gccggtgtcg ctcgaacttg gcggcaagag tccctcgatc
     2701 gtcttccccg atgcaaacga ggattgggtt atcgatggaa ttattgcagc gatgcgcttc
     2761 acccggcaga gccagtcctg caccgcgggc tcccgcctct tcctacaccg cgacatcttc
     2821 gacaactttc ttagtgcgct cgggcaaaag gtcaaagcgc tcaagctcgg tgatccgctt
     2881 gatgagacaa gtgacatagg taccatcatc aacgagaagc aattcaggaa agtgtgtggc
     2941 tacgtcgagg aaggtcttaa gcgacgcgaa gcacgcctcg tgatcggcgg tctcccgccc
     3001 aaggaagggc cccttagcga aggctattac gcaatcccca ccatcttcgc gaacgttgct
     3061 aacgactggc acctggcgcg cgaggagatc tttggccccg tgcttgtcgc cattccttgg
     3121 accgacgaag cggacgcgat tcgcatggcg aatgacagcc attacgggct cgccgccta
//
LOCUS       DASPZD010000298         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1716454, whole genome shotgun sequence.
ACCESSION   DASPZD010000298 DASPZD010000000
VERSION     DASPZD010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716454"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VEM33_05785"
     CDS             <1..655
                     /locus_tag="VEM33_05785"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HYS51368.1"
                     /translation="ERDLMCGAHRPLADAVAKDYETRGVQLTAVYSNCSGKHTGMLGL
                     AKHHGWPTEFYARVEHPVQQRCLKAVADYTDVAAKEIGVAVDGCGVACFALPLRNMAL
                     AYTRIPTAILEPMVLHPELIAGEGRPCTEMMRAHPGRVVAKVGAEGVYSASLIRESLG
                     VTLKVEDGHSAASALAIASVLAELGLKPQPASLLQRPITNSRGETVGEMRVNGGLLK"
     gene            690..1331
                     /locus_tag="VEM33_05790"
     CDS             690..1331
                     /locus_tag="VEM33_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYS51369.1"
                     /translation="MSVEKPTGLDPQTLALVRIAAATATGDEARLRDRMIAARAVHVP
                     PHWVDELLLQSFLNVGYPLALVAFGVWRSVAGPVLDSEKGEPIAHPEWERWTTRGAEA
                     CAEVYGRTFHKLLLNLRALHPSIEPLVLVDAYGKILGRPGLDSKRRELCTLAAIAMQN
                     APRQLHAHLRGALNTGSSREDVDAVLAVVEMDLTTERALKLWEMWADVRGRNL"
     gene            1781..>2327
                     /locus_tag="VEM33_05795"
     CDS             1781..>2327
                     /locus_tag="VEM33_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYS51370.1"
                     /translation="MFIDRVVVQVVAGTGGSGASSFRREKFVPKGGPDGGDGGKGGSV
                     YVKADTNLATLLDYRYHTKWIAERGAHGKGKNMSGKSSDDVYLPVPPGTEVRDADTGE
                     VLGELIQPDQVLLVAKGGRGGRGNQHFATPTHQSPREWEPGEQGEERRLELVLKLIAD
                     VGLLGEPNAGKSTLLSVISAAR"
BASE COUNT          431 a          692 c          816 g          388 t
ORIGIN      
        1 cgagcgcgac ctgatgtgcg gagcgcaccg gccgctggcg gacgcggtcg ccaaggacta
       61 cgagacccgt ggcgtgcagc tcaccgccgt gtactcgaac tgctccggga agcacaccgg
      121 catgctgggc ctggcgaagc atcacggctg gccgaccgag ttctatgcgc gagtcgagca
      181 tccggtgcag cagcgctgtc tgaaagccgt agccgactac acggacgtcg ctgcgaaaga
      241 gattggggtc gccgtcgacg ggtgcggcgt cgcctgtttc gcgctgccgc tgcgcaacat
      301 ggcgctcgcc tataccagga taccaaccgc gatcctcgag cccatggtcc ttcaccccga
      361 gctgatcgcc ggtgaagggc ggccctgcac ggagatgatg cgtgcgcatc cgggacgagt
      421 ggtcgcgaag gtcggcgccg aaggcgtcta cagcgcatcc ctcattcgcg agagcctcgg
      481 cgtcacgctc aaggtcgagg acggccacag cgccgcgtcg gcgctggcga tcgcgtcggt
      541 gctcgcggag ctgggcctca agccgcaacc ggcgtcgctg ctgcaacgac ccatcacgaa
      601 ttctcgcggt gagacggtgg gtgagatgcg ggttaatggg gggcttctga aatgaaacga
      661 ttgcggaatg cggaatgcgg aatgttaagg tgagcgtcga gaagcccact ggcctcgatc
      721 cccagaccct ggcgctcgtg cggatcgccg ccgccaccgc aacgggcgac gaggccaggc
      781 tccgcgaccg gatgatcgcc gcgcgggccg tgcatgtgcc gccgcactgg gtcgacgagc
      841 ttttgctgca gagctttctc aacgtcggct atccgctcgc gttggtggcg ttcggagtgt
      901 ggcgcagtgt ggcggggccg gtgctggaca gcgagaaggg cgagccgatc gcgcatcccg
      961 agtgggagcg ttggaccacg cgcggcgccg aggcgtgcgc cgaggtgtac gggcgcacgt
     1021 tccacaagct gctgctgaat ctgcgggcgc tgcacccgtc gatcgagcct ttggtgctgg
     1081 tggacgcgta cgggaaaatc ctcgggcggc cggggctgga ttcgaagcga cgcgagctgt
     1141 gcacgttggc ggcgatagcg atgcagaacg cgccgaggca gctacatgct cacctgcgcg
     1201 gggccctaaa cactgggtcc agtcgcgaag acgtggatgc ggtccttgcg gtggtagaaa
     1261 tggatctaac cacggaacgt gccctcaagc tctgggaaat gtgggccgat gtgcgcggaa
     1321 ggaatctcta acgtgcggcg tgtcgcgtgc ggcgtagaaa ggcgaggtcg tgcccgcggg
     1381 tcagctgaag atcctcacca aagcgtttgc cttggcgatc gtgcgattgg tggatgaact
     1441 gcctaacagg cgagcgggct gggtcattgg ggatcaactc atgagatcag gtacctccgt
     1501 cggtgccaat taccgctccg catgccgagc gcgctcacgc cgagacttca tagcgaagat
     1561 ggggatcgtt gaggaagagg ccgacgaatc ggactattgg atggagctgg ccgtcgacgc
     1621 tggtcttctg gcgggtccac gagttgaaca attacgcgac gatgcgcagc acattgtagc
     1681 gatgactgtg ctttcaatcc ggcgagcgcg cgaagcccct cgttcaacgc cgcacaccac
     1741 acgccgcacg ctcaatcttc ccgggcgaaa ctgagttcag atgttcatcg atcgcgttgt
     1801 cgttcaagtt gtggcgggaa cgggaggctc cggagcgagc tccttccgtc gcgaaaagtt
     1861 tgtcccgaaa ggtgggcccg acggcggcga tgggggaaaa ggcggcagcg tgtacgtgaa
     1921 agcggacact aacctcgcga cgcttctcga ctacagatac cacacgaagt ggattgcaga
     1981 gcggggcgcg cacggtaagg gcaagaacat gtcggggaag tcgtcggatg acgtctacct
     2041 gcccgtgccg cccggcaccg aggttcgcga cgcggacacc ggcgaagtcc tcggcgagct
     2101 gatccaaccc gatcaagtcc tgctggttgc caagggcggt cgaggtggtc gagggaatca
     2161 acacttcgcg actcccacgc accaatcgcc gcgcgagtgg gagcccggag aacaaggaga
     2221 ggagcggcgt ctcgagctcg tcctcaagct gatcgccgac gtcggcctgc tcggcgagcc
     2281 caacgccggg aagagcacgc tgctgtcggt gatttcggcg gcccgtc
//
LOCUS       DASPZD010000299         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1686013, whole genome shotgun sequence.
ACCESSION   DASPZD010000299 DASPZD010000000
VERSION     DASPZD010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686013"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..>2364
                     /gene="hrpA"
                     /locus_tag="VEM33_05800"
     CDS             293..>2364
                     /gene="hrpA"
                     /locus_tag="VEM33_05800"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HYS51371.1"
                     /translation="MADQRIDELKALLPHCLLHDWVRLGSRLVRLLRDRHHPDKHEAL
                     LRRILDEVRASIALRETRRINAPRVSYPPDLPISVRKDEIVAAIRDRQVIIIAGETGS
                     GKTTQIPKMCLEAGLGVEAMIGCTQPRRVAALSISQRIAEELNVTWGREVGCKIRFDD
                     RSGAHTYIKLMTDGILLAETQGDPALAEYDALILDEAHERSLNIDFLLGYLKGLLVRR
                     QDLKLIVTSATIDTQAFSRHFGDAPIIEVSGRLYPVEVVYQPLDAASEERGDISFVDA
                     AVQATERILCETNFGDVLIFMPGERDIRETSDQLEGRFHGEAEVIPLFGRLSSGDQQR
                     VFASSPRRKIVIATNIAETSLTIPGIRYVIDAGLARISRYNPRTRTRRLPVEAVSQSS
                     ANQRKGRAGRVEAGVCIRLYSEEDFNARPPFTQPEIQRANLAEVILRMKAFSLGDIET
                     FPFVQAPTPASISHGFALLPELGALDDRRELTPLGRDLARLPIDPTIGRMLLQSQREH
                     ATHELLIIAAGLSIQDPRERPLDQKEAAAAAHKKFNDPKSDFLALLNIWNSVHDQWDQ
                     LRTQNARRKFCRQHFLSYLRMREWQDLYSQLHDALENLGTLKLNESNADYDAIHRSIL
                     AGLTGHVARREERNSYKAGGNRLLNVFPGSALFERGEPSKKHAPKPDRSAPKPRSSQP
                     QWVVAGEIV"
BASE COUNT          439 a          846 c          668 g          411 t
ORIGIN      
        1 gcaacctcgt gtattcggtg gataccaaaa aacgcatcac tcacgcccgg ttcacggcgg
       61 atggacagtc cctcatcatt tctggcgcca atggccagcc ccagcggaag gacggtcaat
      121 ggcccccgtg gggaagactg caaatctacc ggattgaggc ttagcattga ttctgggtgg
      181 atgcgagtgt tggggcgccg aatttggccg gtgtcgtcaa cgcgctgccg cagcaagaac
      241 accgttctgc atttgcttgg ttcccaacgc gagttttgtt cactgccttt ccatggctga
      301 tcaacgaatt gatgaactga aggcgctcct gccgcactgc ctcctgcacg actgggtgcg
      361 gctgggatcg cggctcgtcc ggcttttgcg cgaccgccat catcccgaca agcacgaggc
      421 gctgctccgc cgcatcctcg acgaggtgcg cgcctcgatt gccttgcgcg aaacccggcg
      481 cattaacgcc cctcgcgtca gctatccgcc cgacctgccg atctcggtcc gcaaggacga
      541 gattgtcgcg gccattcgcg accgccaggt gatcatcatc gcgggtgaaa ccggctcggg
      601 caaaacgacg cagattccca agatgtgcct cgaagccgga ctgggcgtcg aggccatgat
      661 cggctgcacc cagccgcgcc gcgtcgccgc gctctccatc tcccagcgca tcgcggagga
      721 attgaacgtc acctggggcc gcgaggtcgg ctgcaaaatc cgtttcgacg accgctcggg
      781 cgcgcacacc tacatcaagc tgatgaccga cggcatcctg ctcgccgaga cgcagggcga
      841 cccggcgctc gccgagtacg acgccctcat tctcgacgaa gcccacgagc gctcgctcaa
      901 catcgacttt ctcctcggtt acttgaaggg cctgctcgtg cgccgccagg atctcaagct
      961 catcgtcact tccgccacca tcgacacgca ggcgttctcg cgccacttcg gtgatgcgcc
     1021 gatcatcgag gtctccggac gcctgtatcc cgtcgaggtc gtttaccagc cgctcgacgc
     1081 cgcatcggaa gagcgcggcg acatcagctt cgtcgatgcc gccgtgcagg cgaccgagcg
     1141 catcctctgc gaaacgaatt tcggcgacgt gctcatcttc atgccgggcg aacgcgacat
     1201 ccgcgagacg agcgaccaac tcgaaggccg cttccacggc gaggcggaag tcatccctct
     1261 ctttggccgc ctcagttccg gcgatcaaca gcgcgtcttt gccagttcgc cccggcgcaa
     1321 aattgtcatc gccacgaaca tcgccgaaac ctcgctcacc attcccggca tccgctacgt
     1381 catcgacgcc ggcctcgccc gcatcagccg ctacaacccg cgcacccgca cccggcggct
     1441 gcccgtggaa gccgtctcgc agagcagcgc caaccagcgc aaaggccgcg ccggtcgtgt
     1501 cgaagccggc gtctgcatcc ggttgtattc cgaggaggat ttcaacgccc gcccgccgtt
     1561 cacccagccg gaaatccagc gcgccaatct cgctgaagtc atcctgcgca tgaaggcgtt
     1621 cagtcttggc gatatcgaga cctttccgtt tgtccaggca ccgacgcccg cctccatctc
     1681 gcacggtttc gcgctgctcc cggagctggg cgcgctcgac gatcggcgcg aactcacccc
     1741 cctcggccgc gacctggcgc gattacccat cgaccccacg attggccgga tgctgctcca
     1801 gtcgcagcgc gagcacgcca cgcacgaatt gctcatcatc gcggccggcc tgagcatcca
     1861 ggacccgaga gaacgcccgc tcgatcagaa ggaggccgct gccgccgcgc acaagaagtt
     1921 caacgacccc aagtccgact tcctcgcgct gctcaacatc tggaactccg tccacgacca
     1981 gtgggaccag ctccgcaccc agaacgcgcg ccggaagttt tgccggcaac acttcctttc
     2041 ctacctgcgg atgcgcgagt ggcaggatct ttactcgcaa ctgcacgacg cgctggaaaa
     2101 tctcggcact ctgaagctca acgaaagcaa cgccgactac gatgccatcc atcgttcgat
     2161 cctcgccggg ttgaccggcc acgtcgcccg gcgggaagag cgcaactcct acaaggctgg
     2221 ggggaaccga ctgctcaatg tcttccccgg ttcggcgctc ttcgagcgcg gcgaaccgtc
     2281 gaagaagcac gcgcccaagc ccgaccgctc tgcgcccaag ccgcgctcca gccagccgca
     2341 atgggttgtc gccggtgaaa tcgt
//
LOCUS       DASPZD010000300         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_850327, whole genome shotgun sequence.
ACCESSION   DASPZD010000300 DASPZD010000000
VERSION     DASPZD010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850327"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VEM33_05805"
     CDS             complement(<1..110)
                     /locus_tag="VEM33_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYS51372.1"
                     /translation="MTKPLEGLKVVELGQLIAGPFAGKFFSEFGAEVIKI"
     gene            complement(107..703)
                     /gene="recR"
                     /locus_tag="VEM33_05810"
     CDS             complement(107..703)
                     /gene="recR"
                     /locus_tag="VEM33_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HYS51373.1"
                     /translation="MKSPTALEELIEALRCLPGVGPKSAQRIAYHLLQYDKAGAAKLA
                     QSLNGALQSIRRCGKCNSFTDLELCALCGSPRRDPSQLCVVETPGDLLMMEQTLAYHG
                     LYFVLMGRLSPLDGIGPREIGLDVMQRRLAGGEVKEVIVATNFTNEGEATAHYISEML
                     KDRDLRVTRIARGVPVGGELEYVDTGTLAQAFVERRLL"
     gene            complement(712..1035)
                     /locus_tag="VEM33_05815"
     CDS             complement(712..1035)
                     /locus_tag="VEM33_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HYS51374.1"
                     /translation="MKADIAGLMRQAQQMQENMRKLQDELASVEVEGQSGAGMVKVTM
                     TCRHDVKRVDIDPSLLADDKDMLEDLVAAAVNDAARKVDAAVQEKMSALTGGLGLPPG
                     MKLPF"
     gene            complement(1048..>2010)
                     /locus_tag="VEM33_05820"
     CDS             complement(1048..>2010)
                     /locus_tag="VEM33_05820"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HYS51375.1"
                     /translation="ILAEEKLPGEGGALRLLAEGARGSMRDALSLLDQAIAYSAGNVT
                     EQAVRAMLGTIDDGFLYSILEALARGDPAAVLAIAEDMRSRSVGFDGALQDLGALLHR
                     VALAQGAPEAVDAEGGLGERLLALARALDPEDIQLYYQIAIHGRQDLPFAPDEFAGFT
                     MALLRMLAFSPGARGASESQRNGSAGGEPRGATPARERQPAAKTAFDGDWPKLANSLD
                     LGGIAKQLAQASELERFDGEALELCVPPAARHLAEKSYQEKLRSALQERFGRPVRLTV
                     TIRETTGNSARDRAAAAVSRDAFVRDLVENFDATIVESSIKPVR"
BASE COUNT          328 a          706 c          615 g          361 t
ORIGIN      
        1 tcgatcttga tgacttcggc gccgaactcc gagaagaatt tcccggcgaa aggtccggca
       61 atcagctgtc ccagctcgac caccttcaac ccttccaagg gctttgtcac agcagcctgc
      121 gctcgacaaa agcctgcgca agggtgccgg tgtcgacgta ctccagctcc ccgcccaccg
      181 gcacaccgcg tgcgatccgg gtgactcgca gatcgcggtc cttgagcatc tcgctgatgt
      241 aatgagcggt cgcctcgcct tcattcgtga aattcgtcgc aacgatgact tccttgacct
      301 cgccgccggc caggcgccgc tgcatcacgt cgagcccgat ctcccggggc ccgatgccgt
      361 cgagcggcga aaggcgcccc attagcacga aataaaggcc gtgatacgcc agagtctgct
      421 ccatcatcag cagatccccc ggcgtctcga ccacgcaaag ctgagacgga tcgcgccgcg
      481 gagagccgca caaggcgcac agctcgaggt ccgtgaagct gttgcacttc ccgcagcggc
      541 gaatgctctg caacgccccg ttcagggact gagcgagctt cgccgccccc gccttgtcgt
      601 attgcagcag atggtaggcg atgcgctgag cggacttggg gccgactccc gggagacagc
      661 gcaacgcctc gatcagttct tccagagcgg tgggcgactt catcgcgttc gtcagaacgg
      721 cagcttcatc cctggaggca gaccgagacc gcctgtcaaa gccgacattt tttcctggac
      781 cgccgcgtcg actttgcgcg cggcgtcgtt gaccgccgct gccacgaggt cctcgagcat
      841 gtccttgtcg tcggccagca aggacgggtc gatgtcgact cgcttgacat cgtgcctgca
      901 ggtcatcgtc accttgacca tgcccgcgcc cgactgcccc tccacttcca ccgaggcgag
      961 ctcgtcctgg agcttgcgca tgttctcctg catttgctgg gcctgcctca tcaatccggc
     1021 aatatccgcc ttcatcggtt gcctccgtca ccgcacgggc ttgatcgagg actcgacgat
     1081 ggtcgcatcg aaattctcga ccaggtcgcg tacgaatgcg tcccggctca ctgccgcagc
     1141 cgctcgatcg cgggcgctgt tgccggtggt ttctcttatc gtcacggtca ggcgcacggg
     1201 cctgccgaaa cgctcctgca gcgccgagcg cagcttttcc tggtagctct tttccgccaa
     1261 atgcctggcg gccggaggca cgcagagctc gagagcttcc ccgtcgaagc gctcgagctc
     1321 gcttgcctgg gcgagctgct tcgcgatgcc gcccagatcg agcgaattgg cgagcttggg
     1381 ccagtccccg tcgaaggccg tcttggcggc cggctgccgt tccctggccg gggttgcccc
     1441 acgaggctcg cctcccgccg agccgttccg ctgcgactcg ctggcgccgc gtgcacccgg
     1501 agaaaaggcg agcatacgca gcagcgccat cgtgaagccg gcgaactcgt ccggagcgaa
     1561 cggaagatcc tgccgtccgt gaatcgcgat ttgatagtag agctggatgt cctccggatc
     1621 gagcgcccgc gcgagcgcga gcagtcgctc gcccagccct ccttctgcgt cgaccgcctc
     1681 cggcgcgccc tgagcgagcg cgacgcggtg gaggagcgcg ccgagatcct ggagcgcccc
     1741 gtcgaacccg acgctgcggg atctcatgtc ctccgcgatc gccagcacgg cggccggatc
     1801 gccgcgcgcc agcgcttcga ggatcgaata caggaaaccg tcgtcgattg tgccgagcat
     1861 ggcgcgcacg gcctgctcgg tgacgttccc ggcgctgtac gcgatcgcct gatcgagaag
     1921 cgacaaggcg tcgcgcatgc tgccgcgtgc gccctcggcc agcagcctga gcgcaccgcc
     1981 ttctccggga agcttctcct ccgcgaggat
//
LOCUS       DASPZD010000301         3346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1468792, whole genome shotgun sequence.
ACCESSION   DASPZD010000301 DASPZD010000000
VERSION     DASPZD010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468792"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VEM33_05825"
     CDS             complement(<1..599)
                     /locus_tag="VEM33_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS51376.1"
                     /translation="MTDLFPAPVPVPPLAEALRPKSLDDFVGQQHLLGPGKPLRLAFQ
                     SGKPHSMILWGPPGVGKTTLARLMADAFDAQFIALSAVFSGVKDIKEAVRRAEEALAT
                     SGKGTILFVDEVHRFNKAQQDAFLPFVEQGLLTFVGATTENPSFEVIGALLSRTSMYV
                     LKSLSETELGELFDRAVREVLSGLAFDASARERVIGLADG"
     gene            complement(623..1258)
                     /gene="lolA"
                     /locus_tag="VEM33_05830"
     CDS             complement(623..1258)
                     /gene="lolA"
                     /locus_tag="VEM33_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HYS51377.1"
                     /translation="MALRSGSIAVFLGALLTCAASGAGASSLERFAEFIAKTRTARGQ
                     FEQKIFDRNLKLLQESRGELAFSRPGKFRWNYVKPYAQLIVGDGSRVWIYDEDLKQVT
                     VRKLDQALGSTPAALLAGNNEAMRAFRLSDRGAKDGLEWLEALPRDKDGGFERIRMGF
                     GSSGLEVMELVDSFDQTTVLKFGSLERNPKLDPGLFRFSPPKGADVIGDVK"
     gene            complement(1267..>3346)
                     /locus_tag="VEM33_05835"
     CDS             complement(1267..>3346)
                     /locus_tag="VEM33_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYS51378.1"
                     /translation="WLADMLLYLFGVSAWWWVMLLVVTATWTLRHLEGSSTRDRRSSF
                     IALAGFCLLLAGSSGVEALRLYSLKVALPLAPGGLVGEVVARVLQSGFGFTGATLILL
                     TLCAMTLSLFTGISWLAVMEKLGGVLEGSYLWSLEKWQGWLDRKAGAIAAGKREAMVG
                     EKRGLLIDEHAPIRIESPVVEIPKSSRVQRERQEPLFRELPDTPLPPIKLLDEAASDI
                     EVLAAETLEYTSRLIEKKLGDFGVQVRVLAAYPGPVITRYEVEPAIGVKGSQVVNLVK
                     DLARALSVYSIRVVESIPGKSCMGLEIPNPHRQIVRLSEIVSSRAYHELHAPLTLALG
                     KDIAGNPVVADLARMPHLLVAGTTGSGKSVALNAMILSILYKTEPREVRLILVDPKML
                     ELSAYQDIPHLLTPVVTDMRQAANALVWCMGEVERRYRLMTWIGVRNLSGYNHKVADG
                     EKSGNPLKDPDTLDSAEPQALEKLPYIVVIIDELADLMMVSGRKVEELIARLAQKARA
                     AGVHLILATQRPSVDVITGLIKANIPTRIAFQVSSKVDSRTILDQQGAESLIGQGDML
                     YLPPGSGMSQRVHGAYVADQEVHKVVAYLKSLGRPEYVGGLLEGDETDGGPELGLGEQ
                     GGEADPLYDQAVEIVMRTRRASISLVQRHLRIGYNRAARLIEQMEQAGLVSSMQSNGN
                     REVLVPAKVD"
BASE COUNT          628 a         1087 c         1035 g          596 t
ORIGIN      
        1 ccgtcggcaa gtccgatcac ccgctcgcgc gcgctcgcgt cgaacgcgag tcccgagagg
       61 acttcacgca cggcgcggtc gaacagctcc ccgagctcag tctccgagag actcttgagc
      121 acgtacatcg aggtgcgcga aagcagcgcg ccgatcactt caaaagaggg gttctcggtc
      181 gtggcgccta cgaaggtgag caacccctgc tccacgaacg gcaggaatgc gtcctgctgc
      241 gccttgttga agcggtgcac ttcatccacg aagaggatgg tgccctttcc cgacgtcgcc
      301 agcgcctcct cggcccgccg cacggcctct ttgatatctt taacgcccga aaacacggcg
      361 gaaagggcga tgaactgagc gtcgaacgcg tcggccatga ggcgcgcgag cgtcgtcttc
      421 ccgactcccg gcggtcccca caggatcatg gaatgcggct tgccggattg gaacgcaaga
      481 cgcaagggct tgcccggccc gagcagatgc tgctgcccta cgaaatcgtc gagcgatttg
      541 ggccgcaggg cttcggccag cggcggcacg ggcacaggag cgggaaacag atcggtcatc
      601 cgcttgccgg gcgaagaacc agctacttga cgtcgccgat cacgtcggcg cccttgggag
      661 gcgagaatcg gaagagtccg ggatccaatt tgggattgcg ctcgagtgac ccgaacttga
      721 gtacggtggt ctgatcgaaa ctgtcgacga gttccatgac ttcgaggccg gaagagccga
      781 agcccatgcg gatcctctcg aagccgccgt ccttgtcccg aggcagcgct tcgagccatt
      841 cgagaccgtc cttcgcgccc ctgtccgaga gcctgaacgc ccgcatggct tcgttatttc
      901 ccgcgagaag ggcggctggc gtggaaccga gcgcctggtc gagtttcctc acggtgacct
      961 gcttgagatc ttcgtcatag atccatactc tggagccgtc accgacaatg agctgggcat
     1021 aaggcttcac gtagttccag cgaaatttcc ccggccgcga aaacgcgagc tcgccgcgcg
     1081 actcttgcag cagcttgagg ttccgatcga agattttctg ctcgaattgc ccccttgcgg
     1141 tgcgggtctt ggcaatgaac tcggcgaaac gctccaggct cgatgctccc gcgcccgacg
     1201 ccgcacatgt gagaagcgct cccaggaaga ccgctatgga cccggaacgc aaggccatcg
     1261 cggcggctaa tccactttcg cgggtaccag cacctcgcga ttgccgttcg attgcatgga
     1321 agacacgagc cccgcctgct ccatctgctc gatgagacgt gccgcgcggt tgtagccgat
     1381 ccgcagatgg cgctgaacga gcgagatcga tgcgcgccgc gtgcgcatca cgatctccac
     1441 ggcctgatcg taaagcgggt cggcctctcc cccttgttcg ccgaggccca gctcgggtcc
     1501 gccgtcggtt tcgtcgcctt cgagaaggcc ccccacgtat tcggggcgtc cgagcgattt
     1561 gagataggcg acaaccttgt gcacttcctg gtcggcgacg taggcaccgt gcacacgctg
     1621 ggacattccg ctgccgggcg gcaagtagag catgtcgccc tggccgatca gcgactccgc
     1681 gccttgctgg tcgaggatgg tgcgcgaatc gaccttgctc gagacctgga aggcgatgcg
     1741 cgtcggaatg ttggccttga tcagccccgt aatcacgtcc acggatggac gctgcgtcgc
     1801 gaggatcagg tgcacgcccg cagcgcgcgc tttctgggcc aagcgtgcga tcagctcctc
     1861 caccttcctg cccgatacca tcatcagatc ggcaagctca tcgatgatga cgacgatgta
     1921 gggaagcttc tccagcgcct ggggttcagc cgagtcgagg gtgtccgggt ccttgagcgg
     1981 gttacccgac ttctcgccgt cggcgacctt gtggttgtag cccgatagat tcctcactcc
     2041 gatccatgtc atgaggcggt agcggcgttc cacttccccc atgcaccaga ccagagcgtt
     2101 cgcggcctgc cgcatgtcgg tgaccacggg cgtgagcaga tgcgggatat cctggtaagc
     2161 ggagagctca agcatcttcg ggtcgaccag gatgaggcgc acctcgcgcg gctcggtctt
     2221 gtacagaatc gacagaatca tcgcgttgag ggcgaccgat ttcccggagc cggtcgtgcc
     2281 tgcgaccagc aggtgcggca tccttgcgag atcggcaacc accgggttgc cggcgatgtc
     2341 cttgccgagc gcgagtgtga ggggagcgtg caattcgtga taggcccgcg agctcacgat
     2401 ctcggaaagg cgcacgatct ggcggtgcgg attggggatc tccaggccca tgcaggactt
     2461 gcccgggatc gactcgacga cgcgaatgct atagaccgac agcgcgcgcg caaggtcctt
     2521 caccaggttg acaacctgac tgcctttcac gcctatggcg ggctccacct cgtagcgcgt
     2581 gatcaccggc cccgggtacg cggcgagcac tctgacctga accccgaaat cgccgagttt
     2641 cttctcgatc aagcgcgagg tgtattccag ggtctcggcg gccaagacct cgatgtcgct
     2701 cgcggcttca tcgaggagct tgatcggggg cagcggagtg tcgggcagtt cgcggaagag
     2761 gggttcctgc ctttccctct ggactctgga ggattttgga atctcgacca cgggggactc
     2821 tatccggatc ggcgcgtgtt cgtcgatcag cagcccgcgc ttttccccca ccatcgcctc
     2881 gcgcttccct gcggcaatcg ccccggcctt gcgatccagc cacccctgcc atttttccag
     2941 ggaccagagg tacgaaccct cgagcactcc ccccagcttt tccatcacgg ccagccagga
     3001 aatgcccgtg aatagagaca gagtcatcgc gcaaagcgta agcaggatca gcgtggcgcc
     3061 cgtgaagccg aagcccgact gcagcaccct cgcaacgact tcgccgacca agcccccggg
     3121 ggcaagcggc agcgcgacct tcagcgagta aaggcgcaag gcttcgaccc cgctcgagcc
     3181 ggcaagcagc aggcaaaagc cggcgagcgc gatgaaggat gaccgccggt ccctggtcga
     3241 gctgccctcc agatgccgca gggtccaagt cgccgtcacc acgagcagca tgacccacca
     3301 ccaggctgaa acgccgaaca gatacaacag catatccgcc agccag
//
LOCUS       DASPZD010000302         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1123376, whole genome shotgun sequence.
ACCESSION   DASPZD010000302 DASPZD010000000
VERSION     DASPZD010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123376"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VEM33_05840"
     CDS             <1..338
                     /locus_tag="VEM33_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51379.1"
                     /translation="DQAGGVVWRWKDGDNYYVARANALENNVSLYYTTNGRRNTIKYV
                     DAPVPGNTWHTLRVEFSGKKMRVSLDGKQYIEMEDDHIAGSGAVGVWTKADSVTLFDD
                     FGYGGASAR"
     gene            430..1170
                     /locus_tag="VEM33_05845"
     CDS             430..1170
                     /locus_tag="VEM33_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYS51380.1"
                     /translation="MDGMVLGFFLLAAFLGAVTTGLAGFAMGLVVSGIWLHILTPVQT
                     VTLMVSYSVLLQGYGVWKLRRALAWRRVAPFIIGGVIGVPIGAMLLAHINPAHLRTGV
                     GLVLLLYSIYYLARPALRAVRGGAPADIGIGFLNGLLGGLTGLVGIVVVIWCQLRGWP
                     KDVQRAVFQPVIITTSAMTAGWLGASGAVTADTVKLFLLGLPMLLAGTWVGMKLYGRL
                     DEAGFRKVVLILLLVSGIFLVVPVSTFR"
     gene            complement(1178..1765)
                     /locus_tag="VEM33_05850"
     CDS             complement(1178..1765)
                     /locus_tag="VEM33_05850"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYS51381.1"
                     /translation="MPPVTQTLLIANVAVFLLQSATGDLLVGWFALWPPGYGFWIWQL
                     VTYGFLHGGIAHIFFNMLGLYMFGSDIERLFGSRYFLFYYFACLVAAALTQLVFTAIA
                     GGPPVPTLGASGGLYGLLLAFGMYFPRRMVMLIFPPIPMQARVFVIIFGALELVFGVT
                     GTAAGVAHFAHLGGMFGGWLAIQYRRRGFPFARRR"
     gene            complement(1813..2541)
                     /locus_tag="VEM33_05855"
     CDS             complement(1813..2541)
                     /locus_tag="VEM33_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51382.1"
                     /translation="MEFPNLSEPVTAAMIGALATVAGALVQLRISWRREMKERERGQP
                     ITKKTRRGPVIVVYALLAAAAVGGFALSQYFVSLREGDRDALRADLQAKLSEINATAM
                     RLEQARTNERRQIETEVQRADASRIGEEGAAASVVVGPCPQVVPGARLECTEQSAVRV
                     SVCARVPASATVKEVQLYVRAEDSKQPWQEARVQAGQDAGQARFAEKFTERPDGDAKQ
                     VCQKFANWGSEKSRIARILVKYAP"
     gene            complement(2688..3119)
                     /locus_tag="VEM33_05860"
     CDS             complement(2688..3119)
                     /locus_tag="VEM33_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51383.1"
                     /translation="MRAALPAMIVAVAIGLHAAPACAQSLDYEFFKARIEPIFLKKKP
                     GHTRCYVCHAESNNAFRLEKLPPGAKFWTEEQSRRNFETVSRLVVPGNFAASRLLFMP
                     LAPEAGGNAFHNGGRQFESKDDADWKTLEQWANGQKPAKSK"
     gene            complement(3129..>4215)
                     /locus_tag="VEM33_05865"
     CDS             complement(3129..>4215)
                     /locus_tag="VEM33_05865"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HYS51384.1"
                     /translation="GNIGGATMLTTVRSHCSSLGCLWILLSLAALPAYSGEVRVYATN
                     SAGDSVHVIDPATHKVVQVIEGIETPHGVDFAPDGTRVYISNEADSTLDVVDRKTGRV
                     VKKVPLSGHPNNIAVTKDGSRVVICIAEDKGGLDIVDTRSLTLAKTVPLNGRMHNVYV
                     TADDRYAVAGSVRHKFLSVLDLKTEQVAWDLKLDKGVRPMTLESAPDGSTRRIFVQLS
                     DLSGFAVVDFAQRKEVARVRFPDQPTGYGMAEGRSETPSHGMAVAPDNKSLWVTSVFA
                     NAVFVYSLPELKLLGHVALPELKLPGRAAIGALPNWATFTPDGNLLYVTNSAIRSVSA
                     IDTKAMKVVANIPVGEVPKRINTLVLR"
BASE COUNT          707 a         1309 c         1417 g          782 t
ORIGIN      
        1 aagatcaggc aggcggcgtg gtgtggcgct ggaaggacgg cgacaactac tatgtcgcgc
       61 gcgccaacgc gctcgagaac aacgtttcgc tctactacac gacgaacgga aggcgcaaca
      121 cgatcaaata cgtcgatgcg ccggtaccgg gcaacacctg gcatacgctg cgggtggaat
      181 tctccgggaa gaagatgcgc gtgagtctgg acggcaagca atacatcgag atggaggacg
      241 atcacatcgc gggcagcggc gccgtcggcg tgtggaccaa ggcggacagc gtgacgctgt
      301 tcgacgattt cggctatggc ggggcgtccg cgcgctgaaa ctcacgtgag attttagggg
      361 acacgcagcc tccccttcag atggcagcca tccatccaag cgctggtcac ttcattttgg
      421 gagcgaccca tggatggcat ggttctcggg ttttttctct tggcggcgtt tctcggtgcg
      481 gtgacgaccg gcctagccgg tttcgccatg ggcctcgtag tgtccggcat atggctgcac
      541 atcctcacgc cggtccagac cgtaaccctg atggtgagct acagcgtttt gctgcagggc
      601 tacggcgtct ggaagctgcg gcgtgcgctc gcttggcgca gagtcgcgcc gttcatcatc
      661 ggcggcgtga tcggcgtgcc gatcggcgcg atgctgcttg cgcacatcaa ccccgcccac
      721 ttgcgcaccg gagtgggcct agtgctgctg ctctacagca tctactactt ggcgcgcccg
      781 gcgctcaggg ccgtgcgggg cggagccccc gccgacatcg gaatcggatt tctcaacggg
      841 ctgctcggcg gactgaccgg cctcgtcggg atcgtcgtgg tcatctggtg ccaattgcgc
      901 ggctggccga aggacgtgca gcgcgcggtg tttcagccgg tcatcatcac gacgagcgca
      961 atgaccgcgg gctggctggg tgcgagcggc gcggtcaccg ccgatacggt gaagctgttt
     1021 ctgctcggcc tgccgatgct gctggcgggc acttgggtcg gaatgaagct ctacggacgg
     1081 ctcgacgaag cgggttttcg caaggtcgtg ctgattctgc tgctggtttc cggaatcttt
     1141 ctggttgtcc ccgtatcgac tttccggtga gcccacttca gcgccgccgc gcgaaaggaa
     1201 agccccggcg gcggtactgg atggcgagcc agcccccgaa catgccgccc aggtgcgcga
     1261 agtgcgccac gcccgccgcg gtgcccgtca ccccgaatac gagctcgagc gcaccgaaaa
     1321 tgatcacgaa gactcgcgcc tgcatcggga tcggcgggaa gatcagcatg accatgcgcc
     1381 gcgggaaata catcccgaag gccagcagca gtccgtatag gccaccggaa gcgcccaggg
     1441 tcgggacggg cgggccgcct gcgatcgcgg tgaacaccag ctgggtgagc gctgccgcga
     1501 ccaggcaggc aaaatagtaa aaaagaaagt aacgcgagcc gaagagccgc tcgatgtcgc
     1561 ttccgaacat gtacagcccg agcatgttga agaagatgtg cgcgatgccg ccgtgcagga
     1621 aaccgtaggt gacgagctgc catatccaga atccgtatcc gggcggccac agcgcgaacc
     1681 atcccacgag caggtcgccg gtcgcagact gaaggaggaa gaccgccacg ttggcgatca
     1741 ggagggtttg cgtgacggga ggcatgggca tggccgagaa gtgacttcgc tcggcgttcg
     1801 gtcaagctcc gctcaaggag cgtatttgac gagaatgcgc gcgatgcgcg atttttcact
     1861 gccccagttc gcgaatttct ggcagacctg cttggcgtcg ccgtcgggcc gctcggtgaa
     1921 tttttccgcg aagcgcgcct gtcccgcatc ctgcccggct tgtaccctgg cttcctgcca
     1981 gggctgcttg gaatcctcgg cgcgcacgta gagctgcact tccttgacgg tcgctgacgc
     2041 cggcactctt gcacagaccg atacgcgcac ggcgctctgc tcggtgcatt ccagcctcgc
     2101 cccggggacg acttgcgggc aagggccgac cacgacgctc gctgccgcgc cttcctcgcc
     2161 gatgcgcgaa gcgtccgcgc gctgaacttc ggtttcgatt tgccgacgct cgttggtccg
     2221 ggcctgttcc agccgcatcg cggttgcgtt gatctccgag agcttcgctt gcagatcggc
     2281 gcgcagggcg tcgcggtcgc cttcgcgcag cgacacgaag tattgcgata gcgcgaaccc
     2341 gcccaccgct gcggcggcca gcagggcgta gacgacgatc acgggcccgc gccgggtctt
     2401 cttcgtgatc ggctggccgc gctcgcgctc cttcatttcc ctgcgccagg aaatgcgcag
     2461 ctgtacgagc gcgcccgcca ccgtggcgag ggcgccgatc atggcggcag tgaccggttc
     2521 ggagaggttc ggaaattcca tgggcggatg atacgagatc gggtccggga tgaaaatggc
     2581 gcgtccggcg cgacgtgaaa tgcgtcacgc cgtgaagaag cgcaaggcgt cgcgtcttcc
     2641 gactttccgt tttacgccgt tccgctttct tcggaaggcc gcgcgaatta cttgctcttg
     2701 gccggcttct gcccgtttgc ccactgttcg agcgttttcc agtcggcgtc gtccttcgac
     2761 tcgaattgcc tgccgccgtt gtggaaggcg ttgccgcccg cctcgggcgc gagcggcatg
     2821 aacagaaggc ggctcgcggc gaagttgccg gggaccacca gtctggagac cgtttcgaag
     2881 ttgcggcggg attgctcctc cgtccagaac ttggctcccg gcgggagctt ttccaggcgg
     2941 aaagcgttgt tgctctcggc gtggcagacg tagcagcggg tgtgccccgg cttcttcttc
     3001 aggaagatcg gctcgatgcg cgccttgaag aactcgtaat cgagagactg cgcgcaggcc
     3061 ggagccgcgt gcaggccaat cgcgaccgcc acgatcatcg ccggaagagc cgctcgcatg
     3121 attcaggcct agcgcagcac gagcgtgttg atgcgcttcg gcacttctcc gaccgggatg
     3181 ttcgcgacca ccttcatcgc cttggtgtcg atcgcggata cagacctgat cgcggagttg
     3241 gtgacgtaga ggagattgcc gtcgggcgtg aaggtcgccc agttgggcag cgcgccgatc
     3301 gcagcgcgcc cgggaagctt gagctcgggc agcgcgacgt gtcccagcag cttcagctcc
     3361 ggaagcgagt agacgaagac ggcgttggcg aacacgctcg taacccagag cgacttgttg
     3421 tccggggcga ccgccatgcc gtgggagggg gtttcgctgc gtccctcggc catgccgtag
     3481 cccgtgggct ggtccggaaa ccggacccgc gccacttcct tgcgctgcgc aaaatccacc
     3541 accgcgaacc cgctcaggtc cgagagctgg acgaagatgc ggcgggtcga gccgtcggga
     3601 gccgattcga gggtcatcgg ccgcacgccc ttgtccagct tgaggtccca ggccacctgc
     3661 tcggtcttga ggtcgagcac ggagaggaac ttgtgccgca ccgagcccgc gacggcgtac
     3721 ctgtcgtcgg ccgtcacgta gacgttgtgc atgcgcccgt tgagcgggac ggtcttggcg
     3781 agcgtgaggg accgggtgtc gacgatgtcc aggccgccct tgtcttccgc gatgcagata
     3841 acgacccggg agccgtcctt ggtgaccgcg atgttgttgg gatggccgct caagggcact
     3901 ttcttgacga ccctgcctgt cttgcggtcg acgacgtcga gcgtgctgtc ggcctcgttg
     3961 cttatgtata cgcgcgtgcc gtcgggagcg aagtcgacgc cgtggggcgt ctcgatgccc
     4021 tcgatcacct ggacgacctt gtgggtcgcc gggtcgatga cgtgaacgct gtcgccggcg
     4081 ctgttggtgg cgtagacgcg gacctcgcct gagtacgccg gcaacgccgc cagcgagagc
     4141 agaatccaca ggcacccaag gctgctgcaa tggcttctga cggtcgtcaa catggtggct
     4201 cctccaatgt tgccg
//
LOCUS       DASPZD010000303         8876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1453389, whole genome shotgun sequence.
ACCESSION   DASPZD010000303 DASPZD010000000
VERSION     DASPZD010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453389"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..373)
                     /locus_tag="VEM33_05870"
     tRNA            complement(301..373)
                     /locus_tag="VEM33_05870"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Thr,seq:tgt)
     gene            complement(439..858)
                     /locus_tag="VEM33_05875"
     CDS             complement(439..858)
                     /locus_tag="VEM33_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HYS51385.1"
                     /translation="MAEPKSTKPYLLRAMYEWCVDNGYTPHISVVVDSGTRVPMEYVR
                     NGEIVLNVGPVAASRLRMGNELIECTARFSGVAKDLVIPVATVAAIYAGENGQGMSFE
                     LGKKGAAEQASGEEVAAPPDPNDPPKTGGKPALRRIK"
     gene            complement(981..1580)
                     /locus_tag="VEM33_05880"
     CDS             complement(981..1580)
                     /locus_tag="VEM33_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYS51386.1"
                     /translation="MMNLYSGTTCPFSQRCRIVLYEKGMDFQIIDVDMYNKPEDIAVM
                     NPYNRLPVLVERDLILYEANIINEYIDERFPHPQLMPADPVMRARARLFLFQFEVELF
                     SHIEALENGNQKQQDRARQHVADQLTQMAPVFTKQKYMLGDEFSMLDVAISPLLWRLD
                     YYGVTLPKAAAPLMKYAERLFSRPAFIEALTPSEKVMRK"
     gene            complement(1670..2476)
                     /locus_tag="VEM33_05885"
     CDS             complement(1670..2476)
                     /locus_tag="VEM33_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HYS51387.1"
                     /translation="MKALVVLLALAAQLALAAEESVRLDSAPIDPRDVASLQSGARSF
                     VNYCLNCHAAGMMRYHKLLDLGLTEQQVKENLLFTTDKIGELMNVAMTRKDAKEWLGT
                     APPDLSVIARARGPDWLYTYMRSFYRDPESATGWNNMVFERVAMPHVLWTLSGQQVQE
                     ERKFKNMEQAEAVGRQQKNAWKIDVLTPEQAGKDGERFILKTIRTETAGSLSQIDYDI
                     MVRDLVNFMTWMSEPNQITRKQVGYVVMLVLLVLLVLTYLLYKEFWKDVH"
     gene            complement(2473..3873)
                     /locus_tag="VEM33_05890"
     CDS             complement(2473..3873)
                     /locus_tag="VEM33_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HYS51388.1"
                     /translation="MAAAEKQAVTGAGPFNGLLTWIDQRFPLMQMWRSHLSEYYAPKN
                     FNFWYYFGSLALFVLVIQIVTGIFLTMNYKPDAAQAFASVEYIMREVPWGWWIRYMHS
                     TGASFFFICVYLHMFRAMLYGSYRKPRELLWIFGMLIFLCLMAEAFFGYLLPWGQMSY
                     WGAQVIVNLFDAIPVIGPDLAIWIRGDFVVSDATLNRFFAYHVIAVPLALLALVVAHL
                     LALHEVGSSNPDGVEIKNVKDAKTGRPLDGIPFHPYYTVKDTFGVVVFLIIFSIVVFF
                     APEAGGYFLEHNNFIPADPLKTPEHIAPVWYFTPYYSILRATTDEFVLWVLAPGVVAF
                     ALLIVWSVKSSATRITVGVTAIVLLLGLLTLDAKVWGVVLMGASVMIFFFMPWLDRSP
                     VRSIRYKGPITKTALTLFVIVFFVLGYLGTESVTEGRTLAAQVGTAVYFLFFFLMPWY
                     SKVDKCLPEPERVTWK"
     gene            complement(3878..4480)
                     /gene="petA"
                     /locus_tag="VEM33_05895"
     CDS             complement(3878..4480)
                     /gene="petA"
                     /locus_tag="VEM33_05895"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HYS51389.1"
                     /translation="MSDQSRVNRRRRSLVIASACAGGASAVATAVPFAASMLPSERAV
                     ALGAPVEADISRLNPGEKMTVEWRGQPVWILHRTKEMLEEIKRTDGKVADPKSERKKG
                     ELTPEYARNEYRSIKPEYLVVVGICSHLGCAPQDRLKPGPEAFESEWTGGFYCPCHGS
                     LFDLAGRVYKNKPAPDNLKVPPYTYLSDTRVLIGEDKKGA"
     gene            4788..5945
                     /locus_tag="VEM33_05900"
     CDS             4788..5945
                     /locus_tag="VEM33_05900"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HYS51390.1"
                     /translation="MRNLWLIFAQTATVAVSVLFVVTTLKPEWLGRSGSALQVVELRQ
                     ATSSSGLPARAASYSDAVRKAVPSVVNIFTSKEIKTPRHPFLDDPLFRHFFGDRLEEE
                     PQRAASLGSGVIVSSKGYILTNHHVVEAADEIEVALIDGKKLKAKAVGSDPETDIAVL
                     QVEGGPVPAITFGDADALRVGDVVLAIGNPFGFGQTVTMGIVSALGRNQLGINTFENF
                     IQTDAAINPGNSGGALIDSAGNLVGINTAIYSRSGGSLGIGFAIPASSAKQVMEQIIQ
                     TGGVTRGWIGVEAREITPEMGESFRMGATTGVLIEGVLRGGPAERAGLKPGDILVAIE
                     GKPVNDPNAMLNLVAALVPGKHASIKLRRDNKDVDVQVAVGKRPTQQRRRR"
     gene            complement(5942..6688)
                     /gene="tatC"
                     /locus_tag="VEM33_05905"
     CDS             complement(5942..6688)
                     /gene="tatC"
                     /locus_tag="VEM33_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYS51391.1"
                     /translation="MSTQDTFISHLVELRDRLLRAILAVIVVFLCLFAWARELYALLA
                     KPLIAALPVGGQMIATDVVGVFLVPVKVAFLVAFVIALPYVLYQVWAFVAPGLYVNEK
                     RLVLPLVVASSLLFLLGMSFAYFLVFPVVFKFMASIAPEGVAWMTDIDKYLSFVLTTF
                     MAFGVTFEVPVVVVVLVRVGVVSIDKLKEIRPYVIVGAFVVGAIFTPPDVISQVMLAV
                     PLWLLYELGIVCARIIGRAPAASEAPGGGD"
     gene            complement(6699..7130)
                     /gene="tatB"
                     /locus_tag="VEM33_05910"
     CDS             complement(6699..7130)
                     /gene="tatB"
                     /locus_tag="VEM33_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008160464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HYS51392.1"
                     /translation="MFDVGFSELMVIAVVALIVIGPERLPRVARTMGHLFGRLQRYVN
                     DVKADINREIELEELRKFKTTFEDAARGFEGTVNTTVDSVQSEVSKAETELNSLTNIE
                     NAAAPPSGAPQPAPADAAPQPGEAGREPQGGAPAPPEKTPV"
     gene            complement(7202..7432)
                     /gene="tatA"
                     /locus_tag="VEM33_05915"
     CDS             complement(7202..7432)
                     /gene="tatA"
                     /locus_tag="VEM33_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HYS51393.1"
                     /translation="MGTWSIWHWLVVLLIVLLVFGTKKLRNIGTDLGGAVRGFKDGMK
                     DSSVDKSEGGSSPQSQLGGRTIEGEIKDKAKT"
     gene            complement(7445..7801)
                     /locus_tag="VEM33_05920"
     CDS             complement(7445..7801)
                     /locus_tag="VEM33_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HYS51394.1"
                     /translation="MDAQACVFCKIVRGEIPSRKIYEDADIFAFHDIHPLAPVHFMII
                     PKKHIDSLADCDMSHQAVLGKLLAMAPRLAREQGSTDGFRVIINTGRVGRQEVYHLHV
                     HVIGGPEALGPMLKRN"
     gene            complement(7804..8148)
                     /locus_tag="VEM33_05925"
     CDS             complement(7804..8148)
                     /locus_tag="VEM33_05925"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HYS51395.1"
                     /translation="MSDILERLADVVESRKAGDPEKSYVSRLFARGPDAILKKVGEEA
                     TEAVMAAKDGDRLRIVGETADLWFHCLIMLAQYGLRPADVLAELRRREGISGIDEKAA
                     RADKRRAKAERK"
     gene            complement(8145..8534)
                     /gene="hisI"
                     /locus_tag="VEM33_05930"
     CDS             complement(8145..8534)
                     /gene="hisI"
                     /locus_tag="VEM33_05930"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HYS51396.1"
                     /translation="MAEEWLDRVKWDEQGLVPAIAQEVSTDKVLTLAWMNRESLAKTA
                     ATGEAHYWSRSRMRLWKKGEQSGNVQTVREIRLDCDEDVVLLRVEQGGGIACHTGRYS
                     CFFQKLDNGRWVTVEPVIKDPKEIYGR"
     gene            complement(8548..>8876)
                     /locus_tag="VEM33_05935"
     CDS             complement(8548..>8876)
                     /locus_tag="VEM33_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HYS51397.1"
                     /translation="GGRRPAGLDAVEWARKMQKLGAGEILLTSMDRDGTREGFDLALT
                     RAVAEAVDVPVIASGGVGKLDHLAEGVLEGRADAVLAASIFHFGEFTVREAKEYMAKR
                     GIEIRL"
BASE COUNT         1719 a         2822 c         2697 g         1638 t
ORIGIN      
        1 cggatcggcg gcgatcctgc ccagagcgga acgggcagga attccgggct gttttgcctc
       61 cgacgcaggt gcggcggcgc gctcggggtc ccgggaggca cccgatgccg gggcttttgc
      121 cgaaggcggt gcggccgggc cgcgaagatc gatcgcaacc caaaccgccg ccccgacgag
      181 caatgcaatc aagactgcga ttctcggcgg ccgacgcatc gtgcctcgat gtcctccatt
      241 gaggatttct gtccggacta ttctagtacg actgtattac aagaacacat tgtcgatcgg
      301 tgccgggtag aggagtcgaa cccccgacca actgattaca aatcagctgc tctaccaact
      361 gagctaaccc ggcatcgcga gacattatac gtctgcgacg atcccctacc ccgatgagct
      421 cccgcatacc gccgcgcctt acttgatcct cctcaacgcg ggtttcccgc cggttttcgg
      481 tgggtcattc gggtcgggcg gggctgcgac ttcttctccg ctcgcctgct ctgccgctcc
      541 ctttttcccg agctcgaacg acataccctg cccgttttct cccgcgtaga tcgccgcgac
      601 ggtcgccacg ggaatgacga ggtccttggc gaccccggaa aaacgcgcgg tgcattcgat
      661 gagttcgttt cccatccgca gccggcttgc cgcaacgggt ccgacgttga gaacgatctc
      721 gccgttgcgc acgtactcca tcggcacgcg agtcccggag tcgaccacca ccgaaatgtg
      781 gggcgtgtaa ccgttgtcca cgcaccattc gtacatcgcg cgcaacagat acggcttggt
      841 ggacttgggc tcggccatcg gggcgcagct gtcattctga gcgaagcgaa gaatctgctg
      901 ttgagactag cggcacaaag cagatccttc gtcgcgaagc tcctcaggat gatgcgcaag
      961 atgacggcgc tcctgcgccg tcatttgcgc attacctttt ccgagggcgt caatgcttcg
     1021 atgaacgcag ggcgggagaa caggcgctcc gcgtacttca tgagcggcgc ggccgccttg
     1081 ggcagggtta cgccgtagta gtccagacgc cacaagagcg ggctgatcgc cacatcgagc
     1141 atcgagaact cgtctcccag catgtacttc tgcttggtga acaccggcgc catctgcgtg
     1201 agctggtcgg cgacgtgctg gcgggcgcgg tcctgctgct tctggttgcc gttctcgagc
     1261 gcctcgatgt gggagaaaag ctccacttcg aactggaata gaaacagccg cgcccgcgcg
     1321 cgcatcaccg gatccgcggg catcagctgc ggatggggaa agcgttcgtc gatatactcg
     1381 ttgatgatgt tcgcttcgta gaggatcagg tcgcgctcga ccagcaccgg caggcggttg
     1441 taagggttca tcaccgcaat gtcttccggc ttgttgtaca tatccacgtc gatgatttga
     1501 aagtccatgc ccttttcgta cagcacgatg cggcagcgct gcgagaacgg gcaggtggtt
     1561 ccggagtaga gattcatcat gtttgcttgc cttgtcgccg aattcggatc gatgtgcaga
     1621 tgaaaaaggc gcaggcgccg agcgcgccgc gcctcctgga cggcttctct cagtgcacgt
     1681 ctttccagaa ctccttgtaa aggagatacg tgagaacgag cagcaccaaa agaaccagca
     1741 tcacgacata gccgacctgc ttgcgcgtga tctgattggg ctcggacatc caggtcatga
     1801 agttgaccag atcgcgaacc atgatgtcgt agtcgatctg ggacagcgat cccgccgttt
     1861 cggtcctgat cgtcttgagg atgaagcgct ccccgtcctt gccggcctgc tcaggcgtca
     1921 gcacatcgat tttccaggca ttcttctgct gcctgccgac cgcttccgcc tgctccatgt
     1981 tcttgaactt ccgctcctcc tgcacctgtt gtccggagag cgtccacagc acgtgcggca
     2041 tggcgacgcg ctcgaacacc atgttgttcc agccggtcgc cgactcgggg tcccggtaga
     2101 aactgcgcat gtacgtatac agccaatccg ggccgcgggc gcgggcgatg accgacaggt
     2161 ccgggggcgc cgtgccgagc cattcttttg cgtccttcct cgtcatggcg acgttcatca
     2221 gctccccgat cttgtcggtc gtaaagagca ggttctcctt gacctgctgc tcggtcaaac
     2281 ccagatcgag caacttgtga tagcgcatca tccccgccgc atgacagttc aggcagtagt
     2341 tgacgaagct gcgggcgccc gattgcaggc tcgcgacatc gcgcgggtcg atcggcgcgg
     2401 aatccagtct gaccgactcc tccgcggcga gcgcaagctg cgccgccagc gcgaggagaa
     2461 cgacgagcgc cttcatttcc aggtgaccct ctcgggctcg ggcaggcatt tgtccacctt
     2521 cgaataccac ggcatcagga agaagaacag gaaatacacc gctgtgccga cttgcgcggc
     2581 gagggtgcgc ccctcggtca cggattcggt gccgagatag ccgagcacga agaacacgat
     2641 cacgaagagg gtcagcgccg tcttggtgat gggccccttg tatcggatcg acctgaccgg
     2701 actccggtca agccacggca tgaaaaagaa gatcatgacc gaagcaccca tcagcacgac
     2761 gccccagact ttggcatcca gtgtcagaag tccgagcagc agcacaatcg ccgtcacccc
     2821 tacggtgatc cgggttgcgc tgctcttgac gctccaaacg atgaggagcg cgaatgcgac
     2881 aacgcccggt gcgagtaccc acaatacgaa ctcatcggtc gtggcgcgca ggatcgagta
     2941 gtacggcgtg aagtaccaca cgggcgcgat gtgctccggg gtcttcagcg gatcggccgg
     3001 gatgaaattg ttgtgctcga ggaaatagcc gccggcctcc ggcgcgaaga acacgacgat
     3061 cgagaaaatg atgaggaaga ccaccacgcc gaaggtgtcc ttcacggtgt aataggggtg
     3121 aaacggaatg ccgtcgagcg gcctgccggt cttggcatcc tttacgttct tgatctcgac
     3181 gccatcgggg ttgctcgaac cgacctcgtg cagcgcgagc aggtgcgcga caacgagcgc
     3241 gaggagcgcg agcggcaccg cgatcacgtg gtaggcgaaa aaccggttga gcgtcgcatc
     3301 cgacaccacg aaatcgccgc ggatccagat cgcgaggtcc ggtccgatca cgggaatcgc
     3361 gtcgaagagg ttcacgatca cctgcgcgcc ccagtaggac atctggcccc agggaagcag
     3421 gtagccgaag aaggcctcgg ccatcaggca gaggaatatc agcatgccaa agatccagag
     3481 aagctcgcgc ggcttgcggt aggaaccgta gagcatcgcg cggaacatgt gcagatacac
     3541 gcagatgaag aagaaggagg cacccgtcga gtgcatgtag cggatccacc agccccaggg
     3601 cacctcgcgc atgatgtact cgaccgaggc gaacgcctgg gcggcgtccg gcttgtaatt
     3661 catggtgagg aagattccgg tgacgatctg gatcaccagc acgaagagcg cgagcgaccc
     3721 gaagtagtac cagaaattga aattcttcgg tgcgtagtac tcggaaaggt gcgagcgcca
     3781 catctgcatc agcgggaagc gctgatcgat ccacgtgagc aggccgttga aaggtcccgc
     3841 gccggtcacc gcctgctttt ctgccgcagc catcgctcta tgcccccttc ttgtcctcgc
     3901 cgatcaggac ccgggtgtcc gagagatagg tgtagggcgg gaccttgaga ttgtccgggg
     3961 cgggcttgtt cttgtagacc cggcccgcga ggtcgaaaag cgatccgtgg caaggacaat
     4021 agaaccctcc ggtccactcg ctctcgaacg cctccggacc cggtttgagc cggtcctgcg
     4081 gagcgcagcc gaggtgcgag caaattccga ctacgaccag gtactccggc ttgatcgagc
     4141 ggtattcgtt acgggcgtat tcgggcgtca gctcgccttt cttgcgctcg ctcttggggt
     4201 cggcgacctt gccgtcggtc cgcttgatct cttcgagcat ttccttggtg cgatgaagga
     4261 tccagaccgg ctggccgcgc cactcgacgg tcattttttc cccgggattg agccggctga
     4321 tgtcggcttc gacgggcgcg ccgagcgcga cggcgcgttc cgaaggcaac atcgatgcgg
     4381 cgaagggaac ggccgtggcg accgcggatg caccgcccgc gcaagcactg gcgatgacca
     4441 agctgcgacg cctgcggttg actcgagatt gatcgctcat ggagaaactc tgattgcgaa
     4501 aggcatcgtc gtagagagcg aaattataca gaaagcatcg cgccagacca aattttttgc
     4561 cccaacttat tgattcttat tacgaattca cggactccgc cgattccgcc ggagcaagtg
     4621 cgcgcgcgac gctgtgggcg ccgtctgctg ctgcaaaggc ggcgcgcgca agcgttttgc
     4681 gcaataatcg cgatacaagg gagtccgggt tgcgcctcgc gtaccgcgca ggcgaacggt
     4741 cacggacgag cgtccaagat ctccgccgaa gcccgcccgc tcaatccatg cgcaatctct
     4801 ggctcatctt cgcccagacg gccaccgtcg cagtgagcgt gcttttcgtc gtcacgacgc
     4861 tgaagccgga gtggctcggt cgctccgggt ccgcactcca ggtggtcgaa ttgcgtcaag
     4921 ccacctcttc atctggcctc cccgcgcgcg ccgcctccta cagcgacgcg gtgagaaaag
     4981 cggtcccctc ggtcgtcaac attttcacca gcaaggaaat caagacgccg cgccatccgt
     5041 tcctcgacga ccccttgttc cggcatttct tcggggaccg cctcgaggag gaaccgcagc
     5101 gcgcggcaag cctcggctcg ggcgtgatcg tgagctccaa ggggtacatt ctcaccaatc
     5161 accacgtggt cgaggccgcc gacgagatcg aggtggcgct catcgacggc aaaaaactca
     5221 aggccaaagc cgtcggttcc gatccggaaa cggacatcgc cgtgctgcag gtcgaaggcg
     5281 ggccggtccc ggcgatcact ttcggcgacg ccgatgccct gcgcgtcggc gatgtcgtgc
     5341 ttgccatcgg caacccgttc ggcttcggac aaaccgtgac gatgggcatc gtatcggcgc
     5401 tcggccgcaa ccagctcggc atcaacactt tcgagaattt catccagacc gatgcggcga
     5461 tcaatccggg aaattccggc ggcgcgctca tcgactccgc cggcaacctc gtcggaatca
     5521 acaccgcgat ctactcgcgc tcgggaggat ccctcggcat cggctttgcg atccctgcat
     5581 cgagcgccaa gcaggtcatg gaacagatca tccagaccgg cggcgtcacg cgcgggtgga
     5641 tcggtgtcga ggcgcgggag atcactccgg aaatgggcga gtccttccgc atgggcgcaa
     5701 ccaccggggt cctgatcgag ggcgtgctgc ggggtggacc cgccgaacgg gcggggctga
     5761 agcccggcga tatcctggtc gcgatcgagg gcaaacccgt caatgatccg aacgcgatgc
     5821 tgaacctggt ggcggccctc gtcccgggga agcatgcttc gataaagctg cgccgcgaca
     5881 acaaggacgt cgatgtccag gtcgccgtcg gcaaacgccc gacccagcaa cgacggcggc
     5941 gctagtcgcc acccccgggc gcttcggacg cggcgggagc gcgtccgatg atgcgcgcgc
     6001 acacgatgcc aagctcgtag agcagccaca acggcaccgc aagcatcacc tgggagatga
     6061 cgtcgggggg cgtgaatatc gccccgacga cgaacgcgcc gacgatcacg taggggcgga
     6121 tttccttcag cttgtctatc gagaccacgc ccacccgcac gaggacgacg accaccaccg
     6181 gaacctcgaa ggtcaccccg aatgccatga aggtggtcag cacgaaggag agatatttgt
     6241 cgatgtcggt catccacgcg acgccttccg gggcgatgga tgccatgaac ttgaaaacga
     6301 ccggaaatac cagaaaatac gcaaaagaca ttcccagcag aaacaagagc gagctcgcga
     6361 ccaccagggg cagcaccagg cgcttctcgt tcacatacaa gcccggcgcg acgaacgccc
     6421 acacctggta gaggacatag gggagcgcga tcacgaacgc cacgagaaag gccaccttga
     6481 ccggaacgag aaacaccccg accacgtcgg ttgcgatcat ctggccgcca accgggagcg
     6541 cagcaatcaa cggtttggca agcaacgcgt agagctccct cgcccaggca aacaggcaca
     6601 ggaacacgac gatcacagca agaatcgcac gcaagagccg gtcgcgcagc tcgaccagat
     6661 gggagatgaa ggtgtcctgc gtgctcattc gcggcaaatc agacgggggt tttttccggt
     6721 ggcgcggggg cgcccccttg cggttctcgg ccggcttcgc ccggctgcgg agccgcatcc
     6781 gcgggcgctg gctgcggggc gcctgacggc ggtgcggcgg cgttctcgat attcgtcaag
     6841 ctattgagct ccgtctcggc tttcgacacc tcgctttgaa ccgagtcgac cgtcgtattg
     6901 accgttcctt cgaagccgcg cgcggcgtcc tcgaaagtgg ttttgaattt gcgcagctcc
     6961 tccagttcga tctcgcggtt gatgtcggcc ttgacgtcgt tgacgtagcg ttgcagccgg
     7021 ccgaacaagt ggcccatggt gcgcgccacg cgaggcaggc gctcgggccc gatgacgatg
     7081 agcgccacca cggcgatcac catcagttcg gagaatccga cgtcaaacac ggtgggtgaa
     7141 cgaatcgctc aaaaaagaag ccgggacagc tcccggcatc cggatccggc cgaagacccc
     7201 ttcaggtctt ggccttgtcc ttgatctcgc cctcgatggt gcgcccgccc agctgggatt
     7261 gcggcgagga tccgccttcg gacttgtcca ccgaggaatc cttcatgccg tccttgaatc
     7321 cgcgtaccgc accgcccagg tcggtgccga tattgcggag ttttttcgtg ccgaaaacca
     7381 gcaaaacgat cagcagcacg accagccagt gccaaatgct ccaggtaccc atggcattac
     7441 cctttcaatt gcgcttcagc atcgggccca aggcttccgg gccgccgatg acgtggacgt
     7501 gcaaatgata cacctcctgt cggcccacac gtccggtgtt gatgatgacg cgaaagccgt
     7561 cggtcgaccc ctgctcgcgc gcgaggcgcg gcgccatggc gaggagcttg cccaacacag
     7621 cctggtggct catgtcgcaa tcggccagcg aatcgatgtg tttcttgggg atgatcatga
     7681 aatgcaccgg ggcgagcgga tggatgtcat ggaaggcgaa aatgtcggcg tcctcgtaga
     7741 tttttcggct ggggatctct ccccggacga tcttgcagaa gacgcaagct tgcgcgtcca
     7801 tcgctatttc cgttccgcct tcgcgcgccg cttgtcggcg cgcgcggcct tttcgtctat
     7861 cccggaaatg ccttcgcggc gacgcagctc cgcgagcacg tcggcgggcc gcaagccgta
     7921 ctgtgcgagc atgatcaggc aatgaaacca gagatcggcc gtttcgccga cgatgcgcag
     7981 ccgatcgccg tccttcgcgg ccatcaccgc ctcggtcgcc tcctcgccga cttttttcag
     8041 gatcgcatcg ggtccgcgcg cgaacagccg cgacacgtaa gacttttccg ggtcgccggc
     8101 cttgcgcgat tcgaccacgt ccgccagccg ttcgagtatg tcgctcatcg tccgtaaatt
     8161 tccttcgggt ccttgatcac cggctccacc gtcacccagc gcccgttgtc gagcttctgg
     8221 aaaaagcagc tgtaccttcc ggtgtggcag gcgattccgc cgccctgctc caccctgagc
     8281 agcaccacgt cctcatcgca gtcgagccgg atctcgcgca ccgtctgtac gtttccggac
     8341 tgctcgcctt ttttccacag gcgcattcgc gagcgcgacc agtagtgcgc ttcgccggtc
     8401 gccgcggtct tggcgagcga ctcgcggttc atccaggcga gcgtcagcac cttgtccgtg
     8461 gacacctcct gggcgatcgc ggggacgagg ccctgttcgt cccactttac ccgatcgagc
     8521 cattcctctg ccattgcgtc cgccgattca aagcctgatt tcgattccgc gctttgccat
     8581 gtattccttc gcctcgcgca cggtgaattc gccgaaatga aaaatgcttg cagcgagaac
     8641 cgcgtcggcg cggccctcga gcacgccctc ggcgagatga tcgagcttgc cgacgccacc
     8701 gctggcgatg accggaacat cgaccgcctc ggccaccgcg cgcgtaagcg cgagatcgaa
     8761 gccttcgcgg gtaccgtcgc ggtccatgct cgtgagcagg atctcgcccg cgcccagctt
     8821 ttgcatttta cgcgcccatt cgacggcgtc caatcccgcc ggccggcgtc ccccgt
//
LOCUS       DASPZD010000304         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1311400, whole genome shotgun sequence.
ACCESSION   DASPZD010000304 DASPZD010000000
VERSION     DASPZD010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311400"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VEM33_05940"
     CDS             complement(<1..439)
                     /locus_tag="VEM33_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51398.1"
                     /translation="MVVALGCDLEKAPSGPESSKLSPRFETSVESSAASSFVCDIGGA
                     IWEGDPDATVLHDLAPSGVTRIAFNYNNRNNAARNPITGALTGPGIDMSCKLAEGTHA
                     QFVAIPYLGVPPLLAGLASGDWDAAFAFDQTLEPPGITAAIPHI"
     gene            608..2518
                     /locus_tag="VEM33_05945"
     CDS             608..2518
                     /locus_tag="VEM33_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYS51399.1"
                     /translation="MIELRMLGRVSLINPNAHDVQSLLAQPRRLALLAYLATPPPGMH
                     RRDSLLALFWPELDQAHARNALRQALHVLRRVLGTSAVTSHGDEEVGLDFEQVWCDVA
                     AFERAVADGQLPDAIELYRGGLLEGFFISNAPEFERWLETERDRLRSTAAGAARELAE
                     RAMENGDTAGAAEWARRGLRLAPLDEGLMQRLIEALDRLGDRAGAVRAYDDFAQRLGQ
                     ELEVRPSAETQALLATVRARVQAAPPAARPEARSPPPLAAVPSNRRWGRLAMSVGLPA
                     AAALVLLVGLSVRGRSHPPTPPSRNAQAYDLYLRANMLVNQRQNRENDSVAITLFERI
                     VALDPSFAAAQAGLARAYALRVSEFAPGDTAALQRAQVAAEKALRLDPNLAEAHQAQA
                     RLLWGPSNHFFHERAIQEDRRALSLDSNMDRAHQHLGNIYLHIGLLDEAIVELQKALA
                     ISPREDNALRRIAEARAYQGHYDDALRILSQVDPEANPPYWYYEMAVVLLHLRRSDSA
                     FALIGNYLQTHPEDRGGVVTSARAVWFAAAGDAQRAKQDIQTAVQKGKGYIHFHHAGY
                     HIALAYALLHRPDSAVQWLHRVAEGGMPCYPLFVSDPFLNNIRGDAGFVAFLREQKAQ
                     WERWQATLPPKG"
     gene            2585..>3007
                     /locus_tag="VEM33_05950"
     CDS             2585..>3007
                     /locus_tag="VEM33_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYS51400.1"
                     /translation="MTTASVRGAVLGTDGTPIEAAAVLITNLSTGERWQGLTSNAGRY
                     FVDGVAVGGPYVLEVRRVGFRSQRRTGVTFALGQRFVADWALEPAATELEPVTVEVAA
                     DLIGRTGRAGPVVVVSESALARLPTLSRDFADLAILSPH"
BASE COUNT          499 a          982 c         1003 g          523 t
ORIGIN      
        1 cgatgtgcgg aatggccgcc gtaatgccgg gcggttcaag agtctgatcg aaggcgaagg
       61 cagcgtccca gtcgccgctg gcgaggcctg ctagcagcgg cggcactcct agatagggga
      121 tcgcgacgaa ctgcgcgtgc gttccctctg ccagtttgca gctcatgtct atgccaggtc
      181 ccgtgagcgc gcccgtgatc gggttccgcg ccgcgttgtt ccggttgttg tagttgaagg
      241 cgatccgcgt gacaccgctc ggcgcgagat catgcaggac cgtcgcatcg gggtctcctt
      301 cccagatcgc gccgccaatg tcgcacacga aactagaagc ggcggacgac tcaaccgatg
      361 tctcgaacct cggtgagagc ttcgacgact ctggacccga gggtgctttc tccaaatcgc
      421 agccgagcgc gacgaccacc agagcgatcg ccgctgagta ccttctgcgt gccttcattg
      481 tagacctccc tgaggcgtga ttgcagcgct atcggttgac ggctaggttc tgcggcgcgg
      541 agccatccta aggcggtcgc gttacgttct tgtgctgcca tcgttacggc cacggtaacg
      601 ggtctcgatg attgagctgc ggatgctcgg gagggtgagt ctcatcaacc ctaacgcgca
      661 cgacgtgcag agcctccttg ctcaaccacg tcgcctcgcc ctgctcgcct acctcgctac
      721 gcctccgccg ggaatgcatc gccgtgatag cctgttggcg ctcttctggc cggagctcga
      781 tcaggcgcac gcgcgcaacg cgctgcgcca agccctccac gtactccgtc gtgtccttgg
      841 tacgagcgcc gtcactagcc acggcgacga agaggtggga ctcgacttcg agcaggtctg
      901 gtgcgacgtc gcggcgttcg agcgcgcggt cgcggacggg caactgcccg atgcaatcga
      961 gttgtatcgg ggaggtctgc tcgaaggctt cttcatctcg aacgcgccgg agttcgagcg
     1021 ttggctcgaa acggagcgtg accgactgcg gtcaacggcg gcgggagctg cgcgggagtt
     1081 ggccgagcga gcaatggaga acggggacac agcgggcgca gcggagtggg ccaggcgcgg
     1141 actgcgactc gcaccgctcg acgaaggcct catgcagcgg ctgatcgaag ccctcgaccg
     1201 actgggcgac cgcgcgggcg ccgtgcgagc ctacgacgat ttcgcccagc ggctcggtca
     1261 agaactcgaa gtgcggccct ccgccgagac ccaggcgttg ctggccacag tgcgggcgcg
     1321 cgtgcaggcg gcgccacccg ccgcccggcc cgaggcgcgt tcaccgcctc cgctggcggc
     1381 cgtcccttcg aaccgccggt gggggaggct cgccatgagc gtgggcctcc cggcggcagc
     1441 ggccctggtc ctgctggtcg ggttgagcgt cagaggccgg tcgcatcccc cgaccccacc
     1501 ctccaggaac gcccaggcgt acgacttgta cctgcgcgcc aacatgctcg tcaaccagcg
     1561 tcagaatcga gagaacgact cggtcgcgat cacgttgttc gagcgcatcg tggcactgga
     1621 cccgagtttt gccgcggcgc aagccgggct ggcgcgagcc tatgcgctgc gggtgagcga
     1681 attcgctccg ggggacacgg cggcgctgca acgagctcag gtggccgcgg agaaagcact
     1741 tcgcctcgac ccgaatctcg cggaggccca ccaagcccag gcccgcctgc tgtgggggcc
     1801 ttcaaaccac ttcttccacg aacgagcgat ccaggaagac cgccgcgcct tgagcctcga
     1861 ctcgaacatg gaccgggcgc atcagcatct agggaatatc tacctgcata tcggactgct
     1921 cgacgaagcg attgtcgagc ttcagaaggc gctcgcgatc tcgcctcgcg aagacaacgc
     1981 cttgcggcgc atcgcggagg cgcgcgcgta tcaagggcac tatgacgacg cactaagaat
     2041 cctgagccag gtagaccctg aagccaaccc gccatactgg tactatgaga tggcggtcgt
     2101 gcttctccat ttgcgtcgga gcgactcggc gttcgcgttg ataggaaact acctgcagac
     2161 ccatcccgag gaccgaggtg gtgtcgtgac cagcgcgcgc gccgtgtggt tcgctgcagc
     2221 aggcgacgct cagcgggcca agcaggacat tcagaccgcg gtccagaagg ggaagggata
     2281 tatccacttt catcatgccg gatatcacat cgcattggcg tacgcgctgc tccaccggcc
     2341 cgactcggcg gtgcagtggc ttcatcgagt ggcggagggc gggatgccct gctatccgct
     2401 gttcgtaagt gatccgttcc tgaacaacat ccggggcgat gctgggttcg tcgcgttttt
     2461 gcgggagcag aaggcgcagt gggagcggtg gcaggcgacg ctgccgccca aaggataggt
     2521 tccgacgaac cgcgcgttcc ttctcctcct cctgctcggt gtcgatccgc tcgcagcgca
     2581 aggcgtgacc acggcgtccg tccgcggcgc ggtactcgga accgatggca ctccgatcga
     2641 agcggcagcg gtcctgatca caaacctctc gaccggcgag cggtggcaag gcctcacgag
     2701 taacgccggg cgttacttcg tcgacggtgt cgcggtcggt ggcccgtatg tgctggaggt
     2761 tcgccgcgtg ggctttcggt cgcagcggcg gactggagtc acgttcgcgt tgggccagcg
     2821 attcgttgcc gactgggcgc tcgagcccgc cgcgaccgag ctcgaaccgg tgacagtcga
     2881 ggtcgccgcc gacctcatcg gccgcacggg tcgcgctggg cccgtcgtgg tcgtttcgga
     2941 gtcggcgctc gcacggctgc cgaccctctc gcgcgatttc gcggacctcg ccatcctcag
     3001 tccccac
//
LOCUS       DASPZD010000305         4739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1183470, whole genome shotgun sequence.
ACCESSION   DASPZD010000305 DASPZD010000000
VERSION     DASPZD010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183470"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VEM33_05955"
     CDS             complement(<1..293)
                     /locus_tag="VEM33_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HYS51401.1"
                     /translation="MLRWLKALLALWALSPVLAWAPGPPVVLLTINGAIGPATADYVH
                     RGIEQAGREGAQLVVLRMDTPGGLDTSMRAIIKDILASPVPVAAFVAPGGARAA"
     gene            445..1659
                     /locus_tag="VEM33_05960"
     CDS             445..1659
                     /locus_tag="VEM33_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51402.1"
                     /translation="MSKLPSLIAAVLLCSALPARSQELPEGKGKEMVQAQCTSCHAFR
                     PGGGYTPTGWNTVMRMMTNHGAPIPPDQSATITEYLIKNFPEKAKPAGAVIDGPLKIA
                     MKIWPVATPGSRPHDPLAARDGSLWYTGQMANVLGRVDPKSGKIREYPLKTVHSGPHG
                     LAEDANGNIWYTGNTGALIGKLDPKTGAVTEYKMPDPEAKDPHTLIFDRSGMLWFTVQ
                     NANRIGRLDPKSGEIKLLTPPTPKSRPYGMAFNSKGALFVVQFGANSVAAVDPATLAI
                     REYKLPDPGARPRRIAITSDDIVWYTDYARGYLGRLDPASGKVVEWQSPSGPKSEPYG
                     ISAISDILWYSESGTTPNTVVRFDPKTAKFQSWTIPGGGNIVRNTSVTTDGNFVLANS
                     LVNTVTLVSIPR"
     gene            complement(1670..3709)
                     /locus_tag="VEM33_05965"
     CDS             complement(1670..3709)
                     /locus_tag="VEM33_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYS51403.1"
                     /translation="MKKTKVALAFFSAFFAQHSPAQESETTPQVRIIGHYENAVGTSD
                     AASQGTITPQLIEARPMLRPGEVLEYVPGVIITQHSGDGKANQYFLRGFNLDHGTDFL
                     TTIDHMPVNMPTHAHGQGYSDINFLLPELVSRIAYKKGPYFAEEGDFSSAGAADIDYY
                     TKMPKGIASLGLGSNGYRRAMAADSFDADPGHLLYGFELFHNDGPWINPEDYRKLNGV
                     LRFSEGTNADGYTLSLMGYTGKWNATDQIPQRVVDDQTISRFGAIDPTNGGKTSRYSV
                     SLDRHKTLDNGAVFHMDVYAVKYRLNLFSNFTYFLNDPTNGDQFEQADDRTVVGLTPR
                     WVFNGKWDERQITHKLGLDARRDDINVALYDTTARDRRSPVRSDRVLQTGVGLYYENA
                     IQWTDTFRSIAGVREDFYGARVDNDLGGNSGTTNAHITSPKLSFIFGPFDKTEYFLNY
                     GQGFHSNDARGTTITTATKVPALVRTRGEEIGIRTEVISHLQSSLSLWRLALDSELVF
                     KGDTGTTEASRPSLRRGIEWSNRYIPHDWLLVDLDLSASRAQFTDNDPKGSYIPGAID
                     RVASLGVTVKDLGPWSGTVHARYVGPRPLIEDNSQRSQSSVIFSARASYKVDPKTSVN
                     FDVFNLFNRKSNDIEYYYASRLNNPIQPEPPSGVKDIHFHPAEPRTARLALVMHY"
     gene            3821..>4739
                     /locus_tag="VEM33_05970"
     CDS             3821..>4739
                     /locus_tag="VEM33_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HYS51404.1"
                     /translation="MKADAAPTAPPQRLPAALLKRFTADALLAVGLPAKDAATCAELM
                     VRADLQGADGHGIFRLPQYVRRIKGGAVNVKPKVKIAREAAGMALVDGDNGMGHVVMS
                     FAAKAAIAKAKTAGVAWVGVNRSNHAGPASLYASMPLEHDMIGLYLAVGNANHMAPWG
                     GIDLLLSTNPIAVAAPGNETSIALDMATTVAAYGKVKTAVQRGETLPEGWMIDHQGRP
                     LTDPKRADQGLLVPIGGYKGYGLALVFGLLAGTLNGAAMGSETVDLNADDVTPTNTGH
                     AIVAISIAAFGDVDEFKRRVDKLAREIRRS"
BASE COUNT          851 a         1502 c         1565 g          821 t
ORIGIN      
        1 gccgcgcgcg cgccgcccgg ggcgacgaag gcggcgaccg gcacgggcga ggcgagaatg
       61 tccttgatga tggcgcgcat cgaagtgtcc aggccgcccg gcgtgtccat cctgagcacc
      121 acgagctgcg cgccctcgcg cccggcttgc tcgatcccgc ggtgcacgta gtccgcggtc
      181 gccggcccga tggcgccgtt gatcgtgagc aggacaacgg gggggccggg cgcccacgcg
      241 agaacgggcg agagcgccca cagtgcaagc aaggccttaa gccagcgcag cattttccgg
      301 attggctagt tctgccgcac gatgagcggc gctccaagtg tagcccatac tggacttccc
      361 cctgcgttcg cagtaacttg cgcaaaacaa atagccagca aatcaccaac ctcgcttacg
      421 gctacgcgct gaagaactcg taacgtgtcg aaacttccat ccctgatcgc tgccgtcctc
      481 ttgtgctcgg cgttgccggc tcggagccag gagctgcccg aggggaaggg aaaagaaatg
      541 gtccaggcgc agtgcacgag ctgccacgcg tttcgccccg gcggcgggta cacgcccacg
      601 ggttggaaca cggtcatgcg catgatgacc aaccacggtg cgccgattcc gcccgaccag
      661 agcgctacga tcacggaata cctgatcaag aacttccccg agaaggcgaa gccggccggt
      721 gctgtgatcg acgggccgct gaagatcgcg atgaagatct ggccggtggc gacgcccggc
      781 tcgcggccgc acgatccgct ggcggctcgg gacggatcgc tctggtacac cggccagatg
      841 gccaatgtgc tgggccgcgt cgatccgaag agcggcaaga tcagggaata cccgttgaag
      901 acggtccatt ccggaccgca tggtctggcc gaggatgcga acgggaacat ctggtacacg
      961 ggaaacaccg gagccttgat cggcaagctc gatccgaaga cgggcgcggt caccgagtac
     1021 aagatgcccg acccggaagc gaaggacccg cacactctga tcttcgaccg gagcggcatg
     1081 ctgtggttta ccgttcaaaa cgccaaccgc atcgggcggc tcgacccgaa gtcgggcgag
     1141 atcaagctgc tcacgccgcc cacgccgaaa tcgcgtccct acggcatggc attcaattcg
     1201 aagggcgcct tgttcgtcgt gcagttcggc gccaacagcg tggcagcggt ggatccggcg
     1261 acgctcgcga tccgcgagta caagctgccc gatcccgggg cccggccgcg gcgcatcgcg
     1321 atcaccagcg acgatatcgt ctggtacacc gactacgcgc gcggttatct ggggcggctc
     1381 gatccggcga gcggcaaggt ggtcgaatgg caatctccga gcggccccaa gtccgagcct
     1441 tacggcatct cggcgatcag cgacatcctc tggtacagcg agtccggaac caccccgaac
     1501 acggtcgtgc gcttcgatcc caagacggcg aaattccaga gctggaccat cccgggcggc
     1561 ggcaacatcg tgcgcaatac ctcggtcacc acggacggca acttcgttct tgcgaacagc
     1621 ctggtcaata cggtgaccct ggtcagtatc ccgaggtaga cacgctccct cagtagtgca
     1681 tcaccagcgc caggcgcgcg gtgcgcggct ccgccgggtg gaagtggatg tctttcaccc
     1741 cggaaggggg ttcgggctga atgggattgt tcaggcgcga tgcatagtag tactcgatgt
     1801 cgttcgactt gcggttgaag agattgaaga cgtcgaaatt cacgctggtc ttcgggtcga
     1861 ccttatagga ggcgcgcgcg gagaaaatca ccgaagactg cgagcgctgg ctgttgtcct
     1921 cgatcagagg ccgcgggccg acgtagcgcg catgcacggt ccccgaccag gggccgaggt
     1981 ctttcaccgt cacgcccagt gatgctaccc ggtcgatcgc tcccggaata tagctgcctt
     2041 tgggatcgtt gtccgtaaac tgcgcgcgcg acgccgaaag gtcgaggtcg acgagcagcc
     2101 aatcgtgcgg gatatagcgg ttcgaccact cgatgccgcg gcgcaggctc gggcggctcg
     2161 cctcggtggt gccggtgtcg cctttgaaaa ccaactcgga gtccagggcg agccgccaca
     2221 acgagagcga ggattgcagg tgcgaaatca cttcggtgcg tatcccgatc tcctcccctc
     2281 gcgtccttac cagggccggc accttggtcg cagtggtgat cgtcgtgccg cgcgcgtcgt
     2341 tgctgtggaa gccctgtcca tagttcagga aatattctgt cttgtcgaag ggcccgaaaa
     2401 taaaactgag cttgggcgag gtgatatgcg cgttcgtcgt tcccgagttc ccgccgagat
     2461 cgttgtccac gcgggcgccg tagaaatcct cgcgcacgcc cgcgatgctg cgaaaagtgt
     2521 cggtccactg gatcgcgttc tcgtagtaca ggcccacccc ggtctggagg accctgtccg
     2581 agcgcaccgg gctgcgccgg tcgcgcgccg tagtgtcgta gagcgctacg ttgatgtcgt
     2641 cgcggcgcgc atcgagcccg agcttgtggg tgatctgacg ctcatcccac ttgccgttga
     2701 atacccacct cggcgtcagg ccgacgaccg tgcggtcgtc cgcctgctcg aactgatcgc
     2761 cgttcgtcgg atcgttgaga aagtaggtga agttggagaa cagattgagc cggtacttga
     2821 ccgcgtacac gtccatgtgg aacaccgcgc cgttgtccag cgtcttgtgc cggtcgagcg
     2881 acacgctgta gcgcgatgtt tttccgccgt tggtcgggtc gatcgcgccg aagcgtgaga
     2941 tcgtttggtc gtccaccacg cgctgcggaa tctggtcggt cgcgttccat ttgccggtgt
     3001 agcccatgag ggacagggtg tagccgtccg cgttcgtgcc ctcgctgaag cgcagcacgc
     3061 cgttcagctt gcggtagtcc tccgggttga tccacgggcc gtcgttgtgg aacagctcga
     3121 agccgtagag cagatggccc gggtcggcat cgaaggaatc cgccgccatc gcgcgccggt
     3181 agccgttgct gccgagcccg agcgaagcga ttcccttcgg catcttggtg tagtagtcga
     3241 tgtcggcggc gccggcggaa gaaaagtcgc cttcctcggc gaaatacggg cccttcttgt
     3301 acgcgatccg cgacaccagc tcggggagga ggaagttgat gtcggaatag ccctgcccgt
     3361 gcgcgtgggt cggcatgttc accggcatgt ggtctatcgt ggtgaggaaa tcggtgccgt
     3421 gatcgaggtt gaagccgcgc aggaaatact ggttcgcctt gccgtcgccg ctgtgctggg
     3481 tgatgatcac tccgggaacg tattcgagca cctcgcccgg ccgcagcatc ggccgcgcct
     3541 cgatcagctg cggggtgatg gtgccctggc tcgccgcgtc cgaggtgccg accgcgtttt
     3601 cgtagtgtcc gatgatgcgg acctgcggcg tggtttcact ctcctgcgcg gggctgtgct
     3661 gcgcgaaaaa cgcggaaaaa aacgcgagcg caactttcgt cttcttcaag attgctccca
     3721 tcggatgcag agcgaatcat accgggcgcc ctagcggcgt tgttgagcct tcgctcccgt
     3781 ggtagggttc gggcatgtct cgtcagccgg aacgcgcgca gtgaaagccg acgcagcccc
     3841 gaccgctcct ccgcaacgcc tgcctgcggc cctgctcaag cgcttcaccg ccgatgccct
     3901 gctcgcggtc ggactgcccg caaaggacgc cgccacctgc gccgagctga tggtgcgcgc
     3961 cgacctccag ggcgccgacg gacacggaat tttccgcctg ccgcagtacg tgcgccgcat
     4021 aaaggggggt gcggtcaacg tgaagccgaa ggtgaagatc gcgcgcgaag cggcgggcat
     4081 ggcgctcgtc gacggcgaca acggcatggg gcacgtggtg atgagcttcg ccgcgaaggc
     4141 cgcgatcgcg aaagcgaaga cggcgggcgt ggcctgggtc ggcgtcaatc gcagcaacca
     4201 cgcggggccc gcgtcgctct acgcgtcgat gccgctcgag cacgacatga tcgggctgta
     4261 tctcgccgtc ggcaacgcca accacatggc gccctggggc gggatcgatc tgctgctttc
     4321 gaccaatccg atcgcggtgg cggcaccggg aaacgaaacg agcatcgccc tcgacatggc
     4381 gactacggtc gccgcctacg ggaaggtgaa aaccgccgtg cagcgcgggg aaaccctgcc
     4441 tgagggctgg atgatcgatc accagggcag gccgctcacc gatccgaagc gcgccgatca
     4501 gggcctgctc gtcccaatcg gcggctacaa gggctacggg cttgcgctcg tgttcggcct
     4561 gctcgcgggc acgctgaacg gcgctgcgat ggggagcgaa accgtcgacc tcaacgccga
     4621 cgacgtcacg cccaccaaca ccggccacgc gatcgtcgcg atctcgatcg ccgccttcgg
     4681 cgatgtggac gagttcaagc gccgcgtcga caagctcgcg cgcgagatcc gccgttcga
//
LOCUS       DASPZD010000306         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1713840, whole genome shotgun sequence.
ACCESSION   DASPZD010000306 DASPZD010000000
VERSION     DASPZD010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713840"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2435
                     /locus_tag="VEM33_05975"
     CDS             <1..>2435
                     /locus_tag="VEM33_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYS51405.1"
                     /translation="AARTDPGFDSKAESALILEIAPHLDDFLAELFGVQAEFRALAAR
                     HNELAPLYSIKRQFVQRRAANKVKPEEAAKLDGPALEAQLRKNHLDGRFDELTFAKNV
                     THWLTHEAEHAVALDLALKYAAWALHTPAGRAHVHSGVLFKAPAKIDPHNLLVHAQRN
                     DSEGVVTYTIRPEHIRRRKGFALTDPGTDLVGALDQANYCIWCHTQGKDSCSKGLKEK
                     PSADAPHETVFKKSPFGVTLAGCPLEEKISEFHTLKAQGNALSALAVIAIDNPMVAAT
                     GHRICNDCMKACIYQKQEPVNIPQIETRTLKDVLELPWGFEIYSLLTRWNPLNFRRPL
                     PLPATGYKVLVAGMGPAGFTLAHYLMNEGHAVAGIDGLKIEPLPARFSGVDAHGSRVP
                     VEPIRDVQTLYEGLDDRVMAGFGGVAEYGITVRWDKNFLKLVRLLLERRAQFAMYGGV
                     RFGGTVTVEDAFELGFDHVALCMGAGKPTVLDIPNGLARGVRAASDFLMALQLTGAAK
                     PDSIANMQLRLPVVVVGGGLTAIDTATESLAYYPVQVEKFLMRYETLVAETGEDAVRQ
                     KWDDQEREIAEEFLAHARAIRGERALAAREGRAARVVELLRHWGGVTIAYRRRLIDSP
                     SYTLNHEEVEKALEEGITFCESLSPVAVDVDNYGAARGIRFARESGLGGESAEHWLPA
                     HTVFVAAGTQPNTVLAREDAVHFQLDGRYFQACDEDGRPAKPQRAISKPERADVLLSR
                     FPDGRFVSFFGDLHPSYFGNVVKALGSARQGYPVVSLALAKTPPQSRTPPAEFFASLA
                     GQLLATVHKVERL"
BASE COUNT          425 a          847 c          785 g          378 t
ORIGIN      
        1 ggccgcgcgc acggatcccg gtttcgattc gaaagccgaa tcggcgctga tcctcgagat
       61 cgcgccgcat ctggacgatt ttctcgccga gctgttcggc gtccaagccg agttccgcgc
      121 gctggcggcc cgccacaacg agctcgcccc gctctacagc atcaagcgcc agttcgtgca
      181 gcgccgcgca gcgaacaagg tgaagcccga ggaggcggcc aagctcgacg gaccggcgct
      241 cgaggcccag ctgaggaaga atcacctgga cggacgcttc gacgagctga cgttcgcgaa
      301 aaacgtcacc cactggctca cgcatgaagc cgagcacgcg gttgcgctcg acctcgcgct
      361 caaatacgcg gcatgggcgc tgcacacgcc ggcgggccgc gcgcacgtgc actctggcgt
      421 gctttttaag gcgccggcca agatcgatcc gcacaacctc ctcgtgcatg cgcaaaggaa
      481 cgacagcgag ggcgtcgtca cctacacgat ccgccccgag cacatccgca ggcgcaaggg
      541 cttcgccctc accgatccgg gcaccgacct cgtcggcgcg ctcgaccagg ccaactactg
      601 catctggtgc cacacgcagg gcaaggattc ctgctcgaag gggttgaagg agaaaccttc
      661 cgccgacgcg ccgcatgaaa cggtgttcaa gaaaagcccg ttcggcgtga ccctagccgg
      721 ctgtccgctg gaagaaaaga tctccgagtt ccacacgctc aaggcgcagg gcaacgcgct
      781 ctcggcgctc gccgtgatcg ccatcgacaa tcccatggtt gccgcgactg ggcaccgcat
      841 ctgcaacgac tgcatgaaag cctgcatcta ccagaagcag gagccggtga acataccgca
      901 gatcgagaca cgcacgctga aggacgtcct cgagctgcct tggggcttcg agatctactc
      961 gctgcttacg cgctggaacc cgctcaattt ccggcgcccc cttccccttc ccgcgaccgg
     1021 atacaaggtg cttgtcgccg gcatggggcc cgcaggcttc accctcgccc actacctcat
     1081 gaacgaggga cacgccgtgg ccggtatcga cggcctgaag atcgagccgc tgccggcccg
     1141 cttctccggg gtcgatgctc acgggagccg cgtccccgtc gagccgatcc gcgacgtgca
     1201 gacgctgtac gagggcctcg acgaccgcgt catggcggga ttcggaggcg tcgccgagta
     1261 cgggatcacg gtgcgctggg acaagaattt cctcaagctc gtgcgcctcc tgctcgagcg
     1321 gcgcgcgcag tttgcgatgt acggaggcgt gcgcttcggc ggcacggtca ccgtcgagga
     1381 cgcgttcgag ctcggcttcg accacgtggc gctttgcatg ggcgcgggaa agcccacggt
     1441 gctcgacatc ccgaacgggc tcgcgcgcgg cgtgcgcgcg gcctccgact tcctgatggc
     1501 gcttcagctc accggcgcgg ccaagccgga ctcgatcgcg aacatgcagc tgcgcctgcc
     1561 cgtcgtcgta gtcggcggcg gcttgaccgc gatcgacacc gcgaccgaat cgctcgctta
     1621 ctacccggtt caggtcgaga agttcctgat gcgctacgag acccttgtcg cggagacggg
     1681 cgaggatgcg gtgcgccaga agtgggacga ccaggagcgc gagatcgccg aggagttcct
     1741 cgcccacgcg cgcgcgatcc gcggggaaag ggcccttgct gcacgcgaag gccgcgccgc
     1801 gcgcgtcgtc gagctccttc ggcactgggg cggggtcacc atcgcgtacc ggcgccggct
     1861 gatcgacagc ccttcctata cgctcaatca cgaggaggtc gagaaagcgc tcgaggaagg
     1921 gatcacgttc tgcgaaagcc tgtcgccggt ggcggtggac gtggacaact acggcgccgc
     1981 acgcggcatc cgcttcgccc gggaaagcgg cctcggcggc gagtccgcgg agcactggct
     2041 tccggcgcac accgtgttcg tcgcggccgg gacccagccg aacacggtgc tcgcgcgcga
     2101 ggacgcggtg cacttccagc tcgacggaag atatttccag gcgtgcgacg aggacggaag
     2161 gcccgcgaag ccgcagcgcg cgatttccaa gcccgagcgc gccgacgtgc tgctttcgcg
     2221 ctttcccgac gggcgcttcg tgagcttttt cggcgacctg catccctcct acttcggcaa
     2281 cgtcgtcaag gcactcggct cggcgcgcca gggctacccg gtcgtgtcac tcgcactggc
     2341 aaagacaccg ccgcagtcac gcactccgcc cgccgagttc ttcgcttcgc tcgccggcca
     2401 gctgctcgcc accgtgcaca aggtcgagcg gctta
//
LOCUS       DASPZD010000307         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1238186, whole genome shotgun sequence.
ACCESSION   DASPZD010000307 DASPZD010000000
VERSION     DASPZD010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238186"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /locus_tag="VEM33_05980"
     CDS             complement(<1..1690)
                     /locus_tag="VEM33_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYS51406.1"
                     /translation="MKPYLSRRDFLKTGSALVISFSLAPEAFGQAKPAPLPGSLNNNR
                     MLNAWLRVDADGTVTIFTGKIELGQGIGTALTQIAADELDVDLKRINIVHGDTALTPN
                     EGQTAGSQSVENSGTALRFACAEARDILMNAAAAKLGVAATALSVSDGTCMAPGGKSV
                     AYWDLVADANLKREASAKVKPKAASEHKWVGKSILRRDIPKKMTGGAAYVQDVRLPGM
                     VFGRVVRPPSPGARLVSFDEAAVKHMPGVIAVVRDGDFLGVAAEREEQAIKAREALKK
                     SARWKESESLPPSGDGLYEHLMKSRSVDSVVSEKTSASAAAPAKTLNATYTRPFQVHG
                     SIGPSCAVAQWREGKLTVWTHSQGVFPLRGDLAKAFAIEPANIHCIHAESAGCYGHNG
                     ADDVALDAALIARATSGRPVKLQWMRDDEFQWEPYGSAMVMKLSGGLDAQGNVVSWTH
                     DVWSHPHNNRPGAAGGVNLLASWHLEKPSKAPFPVDPPQPAGGGDRNSIPLYDFPNQK
                     VVKHFLPEMPLRTSALRTLGAYSNVFALESFIDELALAAGADPVEFRMRHLKDPR"
     gene            complement(1687..2154)
                     /locus_tag="VEM33_05985"
     CDS             complement(1687..2154)
                     /locus_tag="VEM33_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYS51407.1"
                     /translation="MADFTLRVNGATRKVSSVAPDTPLLYVLRNDLELNGPKFGCGLA
                     QCGACTVLIDGKSARSCSVSVSAAAKGAITTLEGLGTMEKLHPLQRAFIEEQACQCGY
                     CSNGMIMAAKALLDGNPRPSEREIKQALNDHLCRCASHNRIVRAVQRAAKEMA"
     gene            complement(2267..2677)
                     /locus_tag="VEM33_05990"
     CDS             complement(2267..2677)
                     /locus_tag="VEM33_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYS51408.1"
                     /translation="MIDSLRAPYGALLLRVSMGVLFILHGLYLKAMVFGMAGAGKYFA
                     SMGLPDWFAWVVMIYETLGGLALILGVYTRWVAVFLGVHLLFAGYLGHVANGWVFSAK
                     GGGYEYPLFWAIACFALALVGDGAYALKTRRAAV"
BASE COUNT          489 a          978 c          966 g          514 t
ORIGIN      
        1 cgcgcggatc cttcaggtga cgcatgcgga actcgaccgg atcggcgccc gccgccaatg
       61 caagctcatc gataaaggat tcgagcgcga atacgttgga gtaggcgccg agcgtgcgca
      121 gggccgaagt gcgcagcggc atctccggca ggaagtgctt gaccaccttc tggttgggaa
      181 aatcgtagag cgggatcgag ttgcggtcgc cgcctccggc cggttgcggc ggatcgaccg
      241 ggaacggcgc cttggacggc ttctccagat gccagctcgc cagcagattc acgccgcccg
      301 ccgcgccggg ccggttgttg tgcggatggc tccacacgtc gtgtgtccag ctgacgacgt
      361 tgccctgggc atcgagtccg cccgagagct tcatcaccat cgccgagccg tacggctccc
      421 actggaactc gtcgtcgcgc atccactgca gcttgaccgg gcggccgctg gtcgcgcgcg
      481 cgatgagcgc cgcgtcgagc gcgacgtcgt cggcgccgtt gtggccgtag cagcctgcgc
      541 tttcggcgtg gatgcagtgg atgttcgccg gctcgatagc gaaggccttc gcgagatcgc
      601 cgcgaagcgg aaatacaccc tggctgtgcg tccacaccgt gagcttgcct tcgcgccatt
      661 gcgcaaccgc gcacgatggg ccgatcgagc cgtgaacctg gaacggccgc gtgtaggtcg
      721 cattgagcgt cttcgcgggc gctgcggcgg aagcggaggt cttttcactg accaccgagt
      781 ccacggatct cgacttcatg aggtgctcgt acaggccgtc gcccgagggc gggagcgatt
      841 cgctctcctt ccatcgcgcg gatttcttca gggcttcgcg cgccttgatc gcctgctcct
      901 cgcgctcggc agccaccccg aggaaatcgc cgtcgcgcac caccgcgatc acgccgggca
      961 tgtgcttcac cgcggcctcg tcaaaggaca cgagccgcgc gccgggcgaa ggcgggcgca
     1021 cgacgcgccc gaacaccatt ccgggcaggc gcacgtcctg gacataggcg gcgccgccgg
     1081 tcatcttctt cgggatgtcg cggcgcaaga tgctcttccc cacccacttg tgttccgacg
     1141 cagctttggg cttgacctta gccgacgcct cgcgcttcag gttcgcatcg gcgacgagat
     1201 cccagtaggc gaccgacttt ccgccgggcg ccatgcacgt gccgtcgctc acgctgaggg
     1261 cggtggccgc gacgccgagc ttcgcagccg cagcgttcat cagaatgtcg cgcgcctcgg
     1321 cgcaggcgaa gcgcagcgcg gtgccgctgt tctcgaccga ctggctgccg gcggtctggc
     1381 cttcgttcgg agtgagcgcg gtgtcgccgt ggacgatatt gatgcgcttc agatcgacgt
     1441 cgagctcgtc ggcggcgatc tgcgtgagcg ccgtgccgat gccctgccct agctcgatct
     1501 tgccggtgaa gatcgtgacc gtgccgtcgg cgtcgacgcg cagccaggca ttgagcatgc
     1561 ggttgttgtt gaggctcccg gggagcgggg cgggcttcgc ctggccgaac gcctcggggg
     1621 cgagcgagaa cgaaatgacc agcgcgctgc cggtcttgag gaaatcgcgg cgcgagagat
     1681 agggcttcat gccatctcct ttgccgcgcg ctgcacggca cgaacgatgc ggttgtgcga
     1741 ggcgcagcgg cacaggtggt cgttcaaggc ctgcttgatc tcgcgttcgc tcgggcgggg
     1801 attgccgtcg agcagcgcct tggcggccat gatcatgccg ttgctgcaat agccgcactg
     1861 gcaggcctgc tcctcgatga aagcccgctg cagcgggtgc agcttttcca tagtccccag
     1921 tccctcgagc gtggtgatcg cccccttcgc cgcagccgaa acgctcacgg agcacgagcg
     1981 cgcggatttg ccgtcgatca gcacggtgca ggcaccgcat tgggcgagcc cgcagccgaa
     2041 cttcgggccg ttcagctcca ggtcgttgcg caggacgtaa aggagcggcg tgtcgggcgc
     2101 gaccgaagat acctttcgcg tcgctccgtt cacccgcaat gtgaagtcgg ccatgatctc
     2161 ctccggtcgg ggttcttgtg tttgggcgga agacgaaagc gtacaccacc gaggcggcgg
     2221 agagccagcg ctggcgcgcc gccgcgtcgg cgttccgcgg ctgcgtctag acggcggccc
     2281 ttctcgtctt caacgcatac gcgccgtcgc ccacgagagc gagcgcaaag caggcgatcg
     2341 cccagaacaa cggatattcg tagccgccgc ccttggcgga gaacacccag ccgttggcaa
     2401 cgtggcccag atagcccgcg aagagcaggt gaaccccgag aaagaccgcg acccagcgcg
     2461 tatagacccc gaggatgagc gccagtccgc cgagcgtctc gtagatcatg acgacccagg
     2521 cgaaccagtc gggcagcccc atggacgcga aatactttcc ggctcccgcc atcccgaaca
     2581 ccatggcctt gaggtaaaga ccatgcagaa tgaacaggac gcccatgctg acgcgcagca
     2641 gcaacgctcc gtacggtgct ctaagtgaat cgatcatttt ttcccctttc tgtctttcgg
     2701 cacgcctttt tcgcgcatga ggatgcgctc attcggactt gccggcaacc ccggcagctc
     2761 gcacccccgc gccctcctcg gagcacgaga cttgatgcac ggccttggca tctgtacact
     2821 tcatctgtac atatgaggca cgcatgagac acgtcaaggt caccgagctg cggcaaaagc
     2881 tcccggagta catggcaaag gtccggcgcg gagagcgctt ccgcgtcacc tcccggggcc
     2941 gggtcat
//
LOCUS       DASPZD010000308         6198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1551892, whole genome shotgun sequence.
ACCESSION   DASPZD010000308 DASPZD010000000
VERSION     DASPZD010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551892"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VEM33_05995"
     CDS             complement(<1..925)
                     /locus_tag="VEM33_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYS51409.1"
                     /translation="MALLGNAAVAMWWNVAPEARSEFEDWHSHEHMPERLRIPGFLRG
                     TRWTSPSGEGDYFVIYELAEYETLTSKHYLDRLNNPTPWSTMMMPQHRNMTRSQCRVL
                     CSHGGGAARTLLTLRFSPVPGQANRLRERLSRDVLPALAARAGLIGAHLLQTQTPTGQ
                     AATAEQKIRGGDAVADWILLAGGYDSKAVAALLDNELEEQTLARHGACHERIAGIYRL
                     SFSLTDKDLPSTSAGCDSQPPSIGRFVSQPATRRLPKDADGKKGARGSRTMQEPLSRA
                     QLRVLGLLNRGLTNQEIAKELEIGVGTIRWHL"
     gene            complement(989..1873)
                     /locus_tag="VEM33_06000"
     CDS             complement(989..1873)
                     /locus_tag="VEM33_06000"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYS51410.1"
                     /translation="MEKRKFLVGLIGEGIQESLSPALHEEEARCQGLTLHYQLIDLAR
                     DGCSVADLPRLIDSAEAAGFDGLNITHPCKQAALPLLTGLSEEARAIGAVNTVVFREG
                     GRKGFNTDCSGFAVSFRQELGDTPRRRVLLLGAGGAGAAIAHAVMGLGVEQLFIVDRD
                     HERAEALARRVAGDHPDRVVASADDIAPAAKRADGLIHATPMGMAAYPGLPLDSSLLR
                     PEMWVAEIVYLPLETELLRAARKRGCRTLDGGGMAVWQAVGAFEHFTGIKPDAARMEA
                     HFLKMVSRAGSADTRIPG"
     gene            complement(1875..3143)
                     /locus_tag="VEM33_06005"
     CDS             complement(1875..3143)
                     /locus_tag="VEM33_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HYS51411.1"
                     /translation="MAIVALSLAGLPIGHAMIGGSVLYLFLAGLDMGTAAEQLLNGMY
                     SSFVLLAIPLFILAAEFMTIGSITERLLSFCNAFVGRYRGGLAQVNVVQSIVFSSMSG
                     SALADAASSGKLMQKMMTQDGKYPAAYAAALTAVSSVIAPIIPPSIPLVIYALVSDTS
                     VGYLFLAGVIPGLLLGIAQMLLIAFTARRRNFPVEKPVPLRDIPRISWEAMPALMMPV
                     VLLGCLYSGITTPTEAAALAAFYALLVSAFLYRGVTWGSVYKSLVTSARVTISIGMLI
                     AAAMAFNYVLTVENIPKTLAILLKGHELTPFTFLLLANLILLVLGCFLEGTTILLIIV
                     PVLLPTAHALGVDPVHFGVIAVVNIMIGLCTPPYGLLLFMMVKIAEVPLRDIVREVMP
                     FLGVMIAALALITFIPGLALFLPRIMGYQG"
     gene            complement(3173..3691)
                     /locus_tag="VEM33_06010"
     CDS             complement(3173..3691)
                     /locus_tag="VEM33_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HYS51412.1"
                     /translation="MNPFFGKAGAWLLRRAENFAAGLLAAMFVAFMLQIVFRYLFNFP
                     VGWTSELSVITWLWLVLWGAAFVVKESEEIRFDLLYAAAGRRTRRVMGIITGLSIVIL
                     YVLSLPATVKYVSFMKVEKTSYLKIRFDWLFAIYVIFAVAVIVRYLWILSHLLRGRDP
                     EATDSTKVSSGL"
     gene            complement(3750..4739)
                     /gene="dctP"
                     /locus_tag="VEM33_06015"
     CDS             complement(3750..4739)
                     /gene="dctP"
                     /locus_tag="VEM33_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HYS51413.1"
                     /translation="MDFKIDRRSALKASAALAAAYVLPAGAQAKPTIRFAAVFSDKDI
                     RAGMIQMFAKEVEGDFKVEPFYNGTLFKQGTELVALQRDNLEMGNIAPQDISKQIPAW
                     SILTSAYLFRDANHLNSFFASDLGAQMKKMVEDEVKVKILGPTFFGTRQVGLKPKKKI
                     NTPADMAGVKLRMPPGDAWQLLGRSIGANPTPMAYAEVYTGLQTGAIDGQDNPLPNVQ
                     NMKFYEVMSQIVLTSHLVGYDLLSVNLKTWQSMSPAKQKAFQAAADKAIAWSAAEHLK
                     REAELADGFRKQGLEVYAPNVNAFRAYAQKTYLGSDEAKDWPKGMLEKINALK"
     gene            complement(4752..5594)
                     /locus_tag="VEM33_06020"
     CDS             complement(4752..5594)
                     /locus_tag="VEM33_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYS51414.1"
                     /translation="MKHEFSLAHLTALSLSPPRLVEVAARAGYRYVGLRLNRVTPSEP
                     LYALADDRALMAETKARLADSGVAVLDIELARMGPENDSRSYLPLLEAGAELGAAHIL
                     TQLPDPELNRAAERFASLCDLARPLGLGIDLEFVSWTDTPDLETAAKVLRVVNRTNAG
                     IMVDTLHFARSGSSLDELKKLPREWFRYAQVCDAPAQAPKTRDEIIHAARFERLFPGE
                     GGLGVREILACLPPGIPFALEIPRASLSRLIGAEEYVRLALKAAKSYLDAPVEVIESS
                     PHTS"
     gene            complement(5591..>6198)
                     /locus_tag="VEM33_06025"
     CDS             complement(5591..>6198)
                     /locus_tag="VEM33_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYS51415.1"
                     /translation="VREALPRDAFIVTDVGWNKNGVGQQFPIYEPGTIFTPGGYATMG
                     FGAPAAMGAKLAHPERTVVALVGDGGFGQNPALLATAFEENIAVVWVIMNNYAYGTIA
                     GLEKAHFGTTFGTVFEKDGKPWSPDYAAIAKAYGIEGLKIGSAAEFRPALERALKSKR
                     PVVIDVVMQNEPVPTAGHWNIMDIYSPGRKVNHASTTVTAK"
BASE COUNT         1142 a         1941 c         2010 g         1105 t
ORIGIN      
        1 tcaagtgcca ccggatcgtc ccgaccccga tttcgagttc tttggcgatt tcctggttgg
       61 tcaaaccgcg gttcagcagg ccgagcactc gaagctgcgc cctgctcaga ggctcctgca
      121 tggttcgcga gccccgcgcc cccttcttgc cgtcggcgtc cttcggcagt cgccgcgtgg
      181 cgggctggga gacgaatctg ccgatcgacg gcggctggga gtcgcacccg gcgctcgtcg
      241 aagggagatc cttgtcggtc aatgagaacg acaaccggta aatgcctgca attcgctcat
      301 ggcacgctcc gtgtctcgca agcgtttgct cctcgagttc gttgtccaag agcgccgcca
      361 cggctttcga atcgtagccg ccggctagca atatccaatc ggccactgcg tctccgccgc
      421 ggatcttctg ctcggcggtc gctgcttggc ctgtcggcgt ttgagtttgc aacaagtgag
      481 ccccgatcag gccggcccgc gccgcaagcg ccggcaagac atctcgtgag agccgctctc
      541 tcaagcggtt cgcctgcccc gggaccggcg aaaaccgaag cgtcagcaag gtgcgcgccg
      601 caccaccacc gtggctgcat aggacccggc actggcttct ggtcatattg cggtgctgcg
      661 gcatcatcat ggtggaccac ggcgtcgggt tgttcagccg gtcaaggtag tgtttcgaag
      721 tgagggtctc gtactccgcc agctcgtaga tcacaaaata gtccccttcg ccggacggtg
      781 acgtccagcg cgtcccgcgc aagaaacccg ggatccgaag tctttccggc atatgctcgt
      841 gagagtgcca gtcctcgaac tccgaccgag cttcgggtgc gacattccac cacatcgcca
      901 ctgccgcgtt gccaagaagc gccatcggac cgctcctccg aactctcgct gtacctgccc
      961 tgtctatccg tggaccatca cacgatgatc agcccggaat ccgcgtatcg gcgctgccgg
     1021 cgcgagaaac cattttcagg aaatgcgcct ccatgcgcgc cgcatcgggc ttgatgcccg
     1081 tgaaatgctc gaaggccccc acggcctgcc agaccgccat cccgccgcca tcgagggttc
     1141 ggcagccgcg ctttcgggcg gcgcgcagaa gttcggtctc cagcgggagg tagacgatct
     1201 ccgcgaccca catttccggc cgcagcaagg acgaatccaa gggcagaccg ggataagctg
     1261 ccattcccat cggagtggcg tggatcaggc cgtccgcccg tttcgccgcc ggtgcaatgt
     1321 catcggcaga tgcgaccacc cggtcggggt ggtctcctgc gactctgcgc gccagcgctt
     1381 cggcgcgctc atggtcccgg tcgacgatga agagctgctc cacgcccaag cccatcacgg
     1441 cgtgagcgat ggccgcgccg gcccctcctg cgccgagcag cagcacgcgc cgcctcggcg
     1501 tatcgcccag ctcctgccgg aaggatacgg cgaagcccga gcaatcggtg ttgaagcctt
     1561 tgcgcccgcc ttcgcggaag accaccgtgt tgacggcgcc gatggcgcgt gcctcttcgg
     1621 agagcccggt cagcaggggc agcgcagcct gcttgcacgg atgcgtgatg ttgagaccgt
     1681 cgaagcccgc cgcttcggcc gagtcgatca gccgcggcag atcggcaacg ctgcacccgt
     1741 cccgggcgag atcgatcagc tgatagtgca gagtgagtcc ctggcaccgg gcttcctcct
     1801 cgtgcagcgc gggagagagg gattcctgaa tcccctcgcc gatcaggccg accaggaatt
     1861 tcctcttctc catctcagcc ctggtaaccc ataatccggg gcagaaaaag ggcgagcccc
     1921 ggtatgaagg taatcagcgc aagtgcggcg atcatgacgc cgagaaatgg catcacctcg
     1981 cgcacgatgt cccgcagggg cacctccgcg atcttgacca tcatgaaaag cagcaggccg
     2041 taaggcgggg tgcacaggcc aatcatgatg ttgacgaccg cgatcactcc gaaatgcacg
     2101 gggtcaacgc ccagcgcgtg cgcggtgggg aggagaaccg gaacgatgat cagcaggatc
     2161 gtggttcctt ccaggaagca gccgagaacc aaaaggatga ggttcgcgag caggaggaag
     2221 gtaaacggcg tgagctcgtg ccccttcagc aggatggcga gcgtcttggg tatgttctcg
     2281 accgtaagca cgtagttgaa agccatcgcg gcggcgatca gcatgccgat cgatatcgtg
     2341 acgcgcgcgc tggtcaccag cgatttgtag acgctgcccc aggtgacgcc gcgatagagg
     2401 aaagcggaaa ccagcagagc gtagaaggcg gcgagagcgg cggcttcagt gggcgtggtg
     2461 atcccggagt agaggcagcc gagaaggacc accggcatca tcagcgcggg catggcttcc
     2521 caactgatgc gcggaatgtc gcgcagcggg accggtttct cgaccgggaa atttctccgc
     2581 cgggccgtga acgcgatcag gagcatctgc gcgatgccga ggagcagacc cgggatgacg
     2641 ccggcaagga acaggtagcc gactgacgta tcggagacca gggcgtagat caccagcggg
     2701 atcgagggcg ggatgatggg cgcgatgacc gaggacaccg ccgtaagggc ggcggcatac
     2761 gccgccgggt atttgccgtc ctgcgtcatc attttctgca tcagcttgcc cgagctcgcg
     2821 gcgtccgcga gggcggagcc cgacatgctc gagaagacga tgctttgcac gacgttgacc
     2881 tgggcgaggc cgccgcggta gcgcccgacg aacgcgttgc agaagctcag cagccgttcc
     2941 gtgatgctgc cgatggtcat gaactcggcg gcgaggatga acagcggtat cgccagcagc
     3001 acgaagctcg aatacatgcc gttgagcagc tgctcggcgg cggtccccat atcgaggccg
     3061 gccaggaaaa ggtaaagaac cgatccgccg atcatcgcgt gcccgatcgg cagcccggcc
     3121 aggctgagag cgacgatcgc catgacgcag agcgagaagg ggctggtggg attcacaggc
     3181 cggagctgac cttggtcgaa tcggtggctt cgggatccct gccgcgcagc agatgcgaca
     3241 agatccagag gtagcggacg atcacggcga cggcgaagat cacgtagatc gcgaacagcc
     3301 agtcgaagcg gatcttgaga taggacgttt tttcaacctt catgaaactg acgtatttga
     3361 ccgttgccgg gagcgagagg acatacagga tcacgatcga aagcccggtg atgattccca
     3421 tgacgcgacg cgtccggcgg cccgccgccg catacaagag gtcgaagcgg atttcctcgc
     3481 tttccttcac gacaaaggcg gcgccccaga gcaccagcca cagccaggtg atgacgctca
     3541 gctcggaggt ccagccgacc gggaagttga acagataccg gaaaacgatc tgcaacatga
     3601 acgcgacgaa catggcggcg agcaggccgg cggcgaaatt ctccgcgcgg cgcagcagcc
     3661 atgctccggc tttgccgaag aacggattca ttgttttgct tcccccaaga cctgcaggcc
     3721 cgcgggtgcg accggcgggc atacgggagt tacttcaggg cgttgatctt ctcgagcatc
     3781 cctttcggcc agtccttcgc ctcgtccgag ccgaggtatg tcttctgcgc ataggcgcgg
     3841 aaggcattga cgttcggggc gtagacttcg agaccctgtt tcctgaagcc atcggccaac
     3901 tccgcttcgc gcttcaggtg ctcggccgcg ctccaggcga tcgccttgtc ggccgcagcc
     3961 tggaaggcct tctgcttggc gggcgacatg ctctgccagg ttttcaggtt gacgctgagc
     4021 aggtcgtatc cgacgaggtg cgaggtcagc acgatctgcg acatcacctc gtagaacttc
     4081 atgttctgca cgttgggcag cgggttgtcc tggccgtcga tcgctccggt ctgcagcccc
     4141 gtatagacct cggcgtaagc catgggcgtg ggattcgcgc caatcgagcg gccaaggagt
     4201 tgccacgcat cgccgggagg catcctgagc ttgacgccgg ccatgtcggc aggggtgttg
     4261 attttcttct tgggttttag gccgacctgc cgcgtgccga agaacgtcgg cccgaggatt
     4321 ttcaccttga cctcgtcctc gaccatcttc ttcatctgcg cgccgagatc gctggcaaag
     4381 aaggaattca gatggttggc gtcgcggaac aggtacgccg aagtcaggat cgaccatgcc
     4441 gggatctgct tggaaatatc ctgcggcgcg atattgccca tctccaggtt gtcgcgctgc
     4501 agggcgacca gctccgtgcc ctgcttgaac aaggtcccgt tgtagaaggg ctccactttg
     4561 aaatcgccct cgacctcttt cgcgaacatc tggatcatcc ccgcacgaat gtccttgtcg
     4621 gagaacaccg ctgcgaagcg gatggtgggc ttggcctgcg ctccggcagg cagcacgtag
     4681 gcagcagcaa gagcggcgct tgccttgagc gccgaacgac gatcgatctt gaaatccatg
     4741 tcaacctcct cctatgaagt atgtggactt gattctatga cttccactgg cgcatcgaga
     4801 tacgactttg cggccttgag cgccaagcgg acatattctt ccgcgccaat gagccgcgaa
     4861 agcgaagccc gggggatctc cagcgcgaaa ggaatgcccg gcgggaggca agcgaggatt
     4921 tcgcggacgc cgaggccgcc ttccccgggg aacagacgct cgaagcgcgc ggcgtggatg
     4981 atctcgtccc gggtcttcgg cgcttgcgcc ggcgcgtcgc agacctgcgc gtagcggaac
     5041 cactctcggg gcagcttctt gagctcatcc aggctggagc cggagcgggc gaaatgcagc
     5101 gtgtcgacca tgatcccggc gttggtccgg ttcactaccc gaagcacctt tgcggccgtc
     5161 tccaggtccg gggtgtccgt ccacgacacg aactcgagat cgatgcccag cccaaggggc
     5221 cgggcgagat cgcacagcga ggcgaagcgc tcggcggcgc ggttaagttc cggatcgggc
     5281 agctgggtca ggatgtgagc cgctccaagc tccgcgcccg cttcgagaag aggtaggtag
     5341 ctcctggagt cgttttccgg ccccatacgt gcgagctcga tgtccaggac ggcgacgccg
     5401 ctgtcggcca gccgcgcttt cgtttccgcc atcagcgcgc gatcgtccgc gagcgcatag
     5461 agcggctcgc tgggtgtgac acggttcagg cgcaatccca cgtaccggta gccggcacgg
     5521 gcggcgacct cgaccagccg cggcggagac agtgacaacg cggtcaggtg ggcgagggag
     5581 aactcgtgtt tcacttcgcc gtcaccgtcg tgctcgcgtg gttcactttt cttccggggg
     5641 aatagatatc catgatgttc cagtgccccg cggtcggaac cggctcgttc tgcatcacga
     5701 cgtcgatcac caccggccgt ttggacttga gcgcgcgctc gagggcgggc ctgaactcgg
     5761 ccgcggagcc gatcttcagg ccctcgatgc cgtaggcctt ggcgatggcg gcgtagtcgg
     5821 gggaccaggg tttgccgtcc ttttcgaaaa cggtgccgaa ggtcgtgccg aagtgcgcct
     5881 tctccagtcc ggcgatggtg ccgtaggcgt agttgttcat gatgacccag accaccgcga
     5941 tgttttcctc gaatgcggtg gcgagcagcg ccgggttctg gccgaagccg ccgtcgccca
     6001 ccagcgctac gacggtgcgc tcggggtgcg cgagctttgc gcccatcgcc gccggcgcgc
     6061 cgaaacccat cgtggcgtag ccgcccggcg tgaagatcgt gccgggctcg tagatcggga
     6121 actgctggcc gacgccgttc ttgttccagc cgacgtcggt gacgatgaac gcgtcccgcg
     6181 gcagggcttc gcggacgt
//
LOCUS       DASPZD010000309         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_986613, whole genome shotgun sequence.
ACCESSION   DASPZD010000309 DASPZD010000000
VERSION     DASPZD010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986613"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..2679)
                     /locus_tag="VEM33_06030"
     CDS             complement(19..2679)
                     /locus_tag="VEM33_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51416.1"
                     /translation="MSRIPRSVFFLLFTVSGFAGLIYESIWSHYLKLFLGHAAYAQTL
                     VLALFMGGMAIGSWLCSRWSGAWGNLLRGYAFAEGLTGLAALAFHPVFVAATDAAYAN
                     ILPALGGEIQATLFKWTLAGLLILPQSVLLGMTFPLMSAGLIRRYPTAPGESLAMLYF
                     TNSLGASIGVLVSGFLMIEKLGLPGTVQSAGALNLALAAAVWLLARGTEEPVRARPGA
                     GWDSGGAAPFRFFLAVALLTGAASFVFEIGWIRMLSLVLGSSTHSFELMLSAFILGLA
                     CGGYWLRRRIDSIADPVRFLGIVLVTMGLLALATLPLYGEMFGLMQAVMNALAKTETG
                     YTLFLFSSHGIALAIMFPATFCAGITLPLITYALLRGGNGEESIGAVYSANTLGSILG
                     VFFAAHVGMSLLGLKGLIAAGAAIDAGLGLVLLWRVAEGPRLRMGAAAFSLACFAAVL
                     AGVHLDAYKMASGVFRRGDLYSSSDATMLFHRDGKTTTVSLMDFGTDRSLRTNGKSDG
                     AINMDPDGPRVSDEITMTLTAAIPLAYRPDAASAAVIGIGTGLTTHTLLGSGALRSVE
                     TIEIEPAMAEASRRFSPRNTSAFADPRSHIIFDDAKTFFSTHNRKYDIIVSEPSNPWV
                     SGVASLFTGEFYKLARRHLNQDGILVQWFQMYEIDASLVASVLRALGESFPDYAVYAA
                     TGSDLLIVAGETRTLARPLADLTAMAGVARELRGVYVNSMWDIEVRRLGGKGSLAPLF
                     LSYGVPANSDFHPYLDLHAAKYRFLQRSADDLTGLLAYSIPVVAMLEGRKKSEPEQTS
                     IDGEEYLEALELTRRARYARDFLLLKTAPEPVAIPRSFQKDLELTRARLIECRNPERS
                     DIWFQSLYQLARTLNPMLSPNEARTLWERI"
     gene            2788..>3306
                     /locus_tag="VEM33_06035"
     CDS             2788..>3306
                     /locus_tag="VEM33_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYS51417.1"
                     /translation="MKFSAWFCIFGAAALTACETVQTTQGGLVGVERRQSMSSLISAF
                     QIDRSAVEAYGKVISEADKKNQLNRDSAQVERVRGVANRLIPSTAAFRADAPRWNWEI
                     NVITSPEVNAWCMPGGKIAVYSGLLTKLDPTDEELAAVMGHEIAHALREHGRERASEQ
                     VAQQLARAFAPEI"
BASE COUNT          626 a         1039 c         1127 g          514 t
ORIGIN      
        1 cgagctgcag ggtccgcgct agatacgctc ccagagcgtt cttgcctcat tcggagagag
       61 catcgggttc agcgtgcgcg ccagctggta gagcgattgg aaccagatgt ccgagcgctc
      121 cgggttgcgg cactcgatca gcctcgcgcg cgtcagctcg aggtcctttt ggaacgagcg
      181 cgggatggcg acgggttccg gcgccgtctt cagcagtaga aagtctctcg cgtagcgcgc
      241 gcggcgcgtg agctccaggg cctcgaggta ctcctcgccg tcgatactcg tttgctcggg
      301 ttcgctcttt tttcgtcctt cgagcatggc aacgaccggt atgctgtagg cgagcaggcc
      361 ggtcaggtcg tcggccgacc tttgcaggaa acggtatttt gccgcgtgca ggtcgaggta
      421 cggatggaaa tccgaattgg ccggcacgcc gtagctcagg aacagcggcg caagggaccc
      481 ctttccaccg agcctgcgga cctcgatgtc ccacatcgaa ttcacgtaga cgccgcgcag
      541 ctcccgggcg acaccggcca tcgcggtgag atcggcgagc gggcgcgcga gcgtccgggt
      601 ttcaccggcg acgatcagca ggtcgcttcc ggtggcggcg taaacggcgt agtccggaaa
      661 gctctccccg agcgcgcgca gcaccgaggc gacgagactc gcgtcgatct cgtacatctg
      721 gaaccattgc accaggattc catcctgatt gaggtggcgg cgcgcaagct tgtagaactc
      781 gcccgtgaac aggctcgcga cgccgctgac ccaggggttg gacggttccg agacgatgat
      841 gtcgtacttg cgattgtgag tcgagaagaa cgtcttcgcg tcgtcgaaga tgatgtggct
      901 gcgcggatcg gcgaaggcgc tggtattgcg cggagaaaag cgccgtgacg cctcggccat
      961 cgcgggctcg atctcgatcg tctcgaccga gcgcagggcg cccgatccga ggagcgtgtg
     1021 cgtcgtgaga cccgtcccga tcccgatgac cgccgcgctc gccgcatcgg gacggtaagc
     1081 cagtggaatc gccgccgtga gggtcatcgt gatttcgtcg gagacgcgcg ggccgtccgg
     1141 gtccatgttg atcgcgccgt ccgacttgcc gttggtgcgc aggctccggt cggtgccgaa
     1201 gtccatgagg ctgaccgtcg tcgtcttccc gtcgcggtgg aagagcatgg tggcgtcgct
     1261 cgacgagtag agatccccgc gacggaacac gcccgaagcc atcttgtacg cgtcgaggtg
     1321 cacgccggcg agcacggcgg cgaagcaggc aagcgagaac gcggcggcgc ccattcgcag
     1381 gcgcggccct tcggcgacgc gccacaggag aacgaggccg agccccgcgt cgatcgccgc
     1441 tccggccgcg atcagcccct tcaagccgag gagggacatg ccgacgtgcg ccgcgaaaaa
     1501 tacgccgaga atggagccca gcgtattggc ggaatacacc gcgccgatcg attcttctcc
     1561 gttgccgccc cggagaaggg cataggtgat gagcggcaac gtgatccccg cgcagaaggt
     1621 cgccgggaac atgatggcga gcgcgatgcc gtggctcgag aacaggaaca gcgtgtaccc
     1681 ggtttccgtc ttcgcgagcg cgttcatcac ggcctgcatc aacccgaaca tctcgccata
     1741 gagcggcagc gtggcgagcg cgagcaggcc catcgtcacc agcacgatgc cgaggaaccg
     1801 gaccgggtcg gcgatcgagt cgatgcgccg gcgcagccaa tagcctccgc aggcgagccc
     1861 caggatgaag gccgagagca tcaactcgaa agagtgcgtc gaggacccga ggacgagcga
     1921 gagcatgcgg atccagccga tctcgaagac gaacgaagcc gcgccggtga ggagggcgac
     1981 tgcgagaaag aatctgaaag gcgccgcgcc cccggaatcc caccctgctc cggggcgagc
     2041 cctcaccggc tcctcggtgc cgcgggcgag cagccacacg gcggcggcga gagcgagatt
     2101 caaggccccg gcgctctgca ccgtgccagg cagcccgagc ttctcgatca tgaggaaccc
     2161 gctcaccagc acgccgatcg aagccccgag gctgttggtg aaatagagca tcgcgagcga
     2221 ttcgccgggc gccgtcgggt agcgccggat cagcccggcg ctcatcaggg gaaaggtcat
     2281 gccgagcaaa accgattgcg gcaggatcag gagtccggcg agcgtccact tgaaaagcgt
     2341 cgcctgaatc tcgccgccga gtgcaggcag aatgttcgcg taggcggcgt ccgtcgcggc
     2401 cacgaagacc gggtggaacg cgagggccgc gagccccgtc aagccttccg cgaacgcgta
     2461 gccgcgaagg agatttcccc aggctcccga ccagcggctg cagagccacg acccgatcgc
     2521 catcccgccc atgaacagcg ccagcacgag cgtctgcgcg taggccgcat gcccgagaaa
     2581 aagtttcagg tagtggctcc agatcgattc gtagatgagc ccggcaaacc cggatacggt
     2641 gaacagcaga aaaaatacgc ttcggggaat gcggctcatg tgcggatcgt aggacgcatg
     2701 gtggcgggcg ctggcattat acgggccggg tgtcgcgggg cttgatcact ccgcgcaggc
     2761 gtggacaata catctctcgg agcgcagatg aaattttcgg cgtggttctg tattttcggg
     2821 gcggcggctc tcaccgcatg cgagacggtg cagacgaccc agggcggctt ggtcggggtc
     2881 gagcgccggc aatcgatgtc gtccctgatc tcggctttcc agatcgatcg aagcgccgtg
     2941 gaagcctatg gcaaagtcat ttccgaagcc gacaagaaaa accagctcaa ccgggattcc
     3001 gctcaggtcg aacgggtgcg cggcgtcgcc aaccgcctga tccccagcac ggcggcgttc
     3061 cgcgccgatg cgccacgctg gaactgggag atcaacgtga tcacctcgcc ggaagtcaac
     3121 gcgtggtgca tgcccggcgg caagatcgcc gtgtactccg gccttctgac gaaattggat
     3181 ccgaccgacg aggagctggc tgccgtgatg gggcacgaga tcgcccatgc cttgcgcgag
     3241 cacgggcgcg aacgcgcgag cgagcaggtg gcccagcagc tcgctcgcgc gttcgcgccc
     3301 gagatc
//
LOCUS       DASPZD010000310         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_915403, whole genome shotgun sequence.
ACCESSION   DASPZD010000310 DASPZD010000000
VERSION     DASPZD010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915403"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1150
                     /locus_tag="VEM33_06040"
     CDS             179..1150
                     /locus_tag="VEM33_06040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYS51418.1"
                     /translation="MPARKLNRQDLRDAAEKYKNWGKWGPNDEIGTLNHTRPEDIVAA
                     ARLVKKGKVISLALNFDNNGPQGAKSKYPSMGRINPVHTMLRTGTDAYSGVLDHRGIR
                     AADDMVVMPLQCGTQWDGLGHVFYENSMWNGYDCREVTSNGAQKCGIEKTKNRMVGRG
                     VFLDVPRAQGKRYLEDGYAITCADLDKTAERQGVKMKRGDFIVVRTGQMEAKLDAGSW
                     DGYPGGDAPGFAFETLEWVQRTELAALASDTWGCEVRPNETEAGINQPWHWITIPMMG
                     MTMGEIFYVKELAEDCAADKVYEFLFVAPAIPITGAVGSPTNPLAIK"
     gene            1159..2838
                     /locus_tag="VEM33_06045"
     CDS             1159..2838
                     /locus_tag="VEM33_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYS51419.1"
                     /translation="MAKMTGAQCLADMLAGYGVTHVFMVPAVLRKTFAEMERRTQIKR
                     IHTHGEKSAAYMADGYARASGRPGICMAQVIGALNLAAGLRDAHLAHSPVIAFTGGRD
                     PKTKFRRVYQEVDDVPAFEPVTKFNATVDDVARFPDMVRQAFRVAVSGTPGPVHLQFR
                     GNEGQVDAEEADMEPLVEQQFARVPPFRPEPEDGSVRAALRILQEAERPVIVAGGGVR
                     ASGAARDLVALAEKLSIPVATSLNGKDSIPGVHPLSVGVVGTYSRESANRVVGQADLV
                     CFIGTETGGMTTHFWAVPKIGVAAIQIDSDPEALGRNYPLKASVLGDAKVVLAKMLEH
                     ADAKTAAKRRPWVEKAQATVKEWYAKYKPALESEAVPIRPERMCGELTKNVPDDAIVV
                     VDTGHAGMWMGGMYDVRAPTQSYMRSAGHLGWAFPAGLGAKCGAPERPVVVFTGDAGL
                     WYHIGEIETAVRWKINSVTVVNNNASGNQSKRGFDRVYGGQQTERARELWTYRPVNFA
                     KVAEDIGALGIRVEKPAELGPAIQRALKADRPVIVDVATDIEALAPVAVTE"
     gene            3033..4319
                     /locus_tag="VEM33_06050"
     CDS             3033..4319
                     /locus_tag="VEM33_06050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYS51420.1"
                     /translation="MKLRSFLRTSLLGVAFFLTGPHPATAQDGAAIDAVLRAAIERKD
                     VPGVVALVVNRDRVLYQGGFGVADVGTGRELTADALFRIASMTKPITSLALMQLVEQG
                     KLGLDDPAEKYLPELAGLKVFESFDAATGEYKLRPATKRPTVRHFLTHTSGLAYPFTS
                     ATWRDFKPKPGETYPFGGPLLVEPGERWHYSTSTDVVGRLVEVISGQRLEDYFRQHIF
                     APLKMTDTSYNVPQEKAPRLVAAQQRAGERMDGAVVLQSPQPGLTIAAPIGGGGLAST
                     AGDYGRFMRMLLNGGALDGARVLKAQTVALMGQNHIGAIGVPALKSALPRSADFNFIA
                     DGRDKFGLGFLITTDQVPGKRSPGSLSWGGINNTYFWVDPSRGIGGVIMMQYLPFADP
                     KALALHEAFERAAYRLAAAQPTAARDERLSPVALAK"
BASE COUNT          839 a         1447 c         1521 g          690 t
ORIGIN      
        1 gttctgcgcg aggcagatac cggcgagcgc ccacgcggcg agggcgacgg agagttttct
       61 cctcatgaat ttcctccttc tgctttcggt catcttatca tcgcccatgc ggcgcgactc
      121 aggctgcgat aaagtagcgg ctcgcgtttt ctccccacga taggaaaaag gaatttccat
      181 gcccgcgcgc aagctcaacc gccaggatct ccgcgatgcc gcggagaaat acaagaactg
      241 gggcaagtgg ggtcccaatg atgaaatcgg cacgctgaac cacacgcggc ccgaggacat
      301 cgtcgccgcg gcgcgcctgg tgaaaaaggg gaaggtcatc tccctcgcgc tcaacttcga
      361 caacaacggc ccccagggcg cgaagagcaa gtacccctcg atgggccgga tcaacccggt
      421 tcacacgatg ctgcgcacgg gcaccgacgc ctattccggc gtgctcgacc accgcggcat
      481 ccgcgccgcg gacgacatgg tcgtcatgcc cctgcagtgc ggcacgcaat gggacggcct
      541 gggccatgtc ttctacgaga actccatgtg gaacggctac gactgccgcg aggtcacctc
      601 caacggcgcg cagaaatgcg gaatcgagaa gaccaagaac cgcatggtcg gacgcggcgt
      661 gttccttgac gtacctcgcg cgcaagggaa gaggtatctg gaggacggtt atgccatcac
      721 ctgcgccgat ctcgacaaga cggccgagag gcagggcgtg aagatgaagc gcggggactt
      781 catcgtcgtg cgcaccgggc agatggaagc gaagctcgac gccggcagct gggacggcta
      841 tcccggcgga gacgcgccgg gattcgcgtt cgagacgctc gagtgggtgc agcgcaccga
      901 gctcgcggcg ctcgcctccg acacctgggg ctgcgaggtg cggccgaacg agaccgaggc
      961 cggcatcaac cagccctggc actggatcac gatcccgatg atgggcatga ccatgggcga
     1021 gatcttctac gtgaaggaac tcgccgaaga ctgcgccgca gacaaggtgt acgagttcct
     1081 gttcgtcgcg ccggcgatcc cgatcacggg cgcggtggga tcgccgacca atccgctcgc
     1141 catcaagtag gggcgacgat ggcaaagatg accggcgccc agtgcctcgc cgacatgctc
     1201 gccggctacg gcgtcacgca tgtcttcatg gtccccgcgg tgctgcgcaa gaccttcgcc
     1261 gagatggagc gcaggaccca gatcaagcgc atccacaccc acggcgaaaa atccgccgcc
     1321 tacatggccg acggctacgc gcgcgcctcg ggcaggccgg ggatctgcat ggcccaggtg
     1381 atcggcgcgc tcaatctcgc cgcgggcctg cgcgacgcgc atctcgcgca ctcgccggtc
     1441 atcgctttca cgggaggccg cgatccgaaa acgaaattcc gcagggtcta ccaggaggtc
     1501 gacgacgtgc ccgccttcga gccggtgacc aagttcaacg cgacggtcga cgacgtcgcg
     1561 cgctttcccg acatggtgcg ccaggcgttc cgcgtcgcgg tgtcggggac gccggggccc
     1621 gtgcatctgc agtttcgcgg caacgagggg caggtcgacg ccgaggaagc ggacatggag
     1681 cctctcgtcg agcagcaatt cgcgcgcgtg ccgccgtttc gtcccgagcc ggaggacgga
     1741 agcgtgcgcg ctgcgctcag gatcctccag gaggccgagc ggcccgtcat cgtcgcaggg
     1801 gggggagtgc gcgcatcggg cgcggcaagg gacctcgtcg ccctcgcgga gaagctttcg
     1861 attccggtcg cgacctcgct caacggcaag gattccatcc ccggggtgca tccgctctcg
     1921 gtcggcgtcg tgggaacgta ttcgcgcgag agcgcgaacc gggtggtggg gcaggcggac
     1981 ctcgtgtgct tcatcggcac ggagaccgga gggatgacca cgcacttctg ggcggtgccg
     2041 aagatcggcg tcgccgcgat ccagatcgac agcgatccgg aagcgctcgg gcgcaactac
     2101 ccgctcaagg cgtccgtgct cggcgacgcg aaggtggtgc tcgcgaagat gctcgagcac
     2161 gccgacgcga agaccgcggc gaagcgcagg ccctgggtgg agaaggcgca ggcgaccgtc
     2221 aaggagtggt acgcgaagta caagccggcg ctcgagtcgg aggccgtgcc gatccgcccg
     2281 gagcgtatgt gcggcgagct cacgaagaac gttccggacg acgcgatcgt cgtcgtcgac
     2341 accgggcacg cgggcatgtg gatgggaggg atgtacgacg tgcgcgcgcc gactcagagc
     2401 tacatgagga gcgccgggca cctcggctgg gcgttcccgg cggggctcgg agcgaaatgc
     2461 ggagcgcccg agcggcccgt cgtcgtgttc accggcgacg ccggcctctg gtatcacatc
     2521 ggcgaaatcg aaacggcggt ccggtggaag atcaactcgg tcaccgtcgt gaacaacaac
     2581 gcgagcggaa atcagtccaa gcgcggcttc gaccgcgtct acggcggcca gcagacggaa
     2641 cgggcgaggg agctctggac ctaccgcccg gtgaatttcg cgaaggtcgc cgaggacatc
     2701 ggagcgctcg ggatccgcgt cgagaaacct gccgagctgg gaccggcgat ccagcgcgcg
     2761 ctcaaggcgg accgcccggt gatcgtcgac gtcgcgaccg acatcgaagc gctcgcgccg
     2821 gtagctgtaa cggagtaagg ctccatcatc gcatcgaacg cgcgcttggc ggcggcgcgg
     2881 tcgggatcgg tgacccgacg ccgcggtaaa gacgttggga aacgggtggg acattatgcg
     2941 ggtcgccgct tttggccgaa aggggctctt gacgctcgcc tgactacggc cgcacactgg
     3001 ccgtgcggtg caaaccgcaa aaaggggagg tcatgaaact gcgaagcttc ctgcgtacgt
     3061 cacttctggg cgttgcgttc ttcctcactg gcccccatcc cgcaacggca caagatggtg
     3121 cggcgatcga tgccgtactg cgcgcggcga tcgaacgcaa ggacgtgccc ggcgtggtcg
     3181 cgctggtggt gaatcgcgac cgggttctct accagggtgg cttcggcgtg gcggatgtcg
     3241 gcaccgggcg cgagctcact gcggacgcgc ttttccgcat cgcctcgatg accaagccga
     3301 tcacctcgct cgcgctcatg caactcgtag agcagggcaa gctcggcctc gatgatccgg
     3361 ccgaaaaata tctgcctgag cttgccggcc tgaaggtttt tgagtcgttc gacgcggcca
     3421 ccggcgaata caagctccgc ccggccacga agcgcccaac cgtcaggcac ttcctcaccc
     3481 atacctcggg cctcgcctat ccgttcacca gcgcaacatg gcgcgacttc aagccgaagc
     3541 caggcgaaac ttatccgttc ggaggcccgt tgctggtcga gccgggtgag cgctggcact
     3601 acagcacgag taccgacgtc gtggggcggt tggtcgaggt gatctccgga caaagactcg
     3661 aagattactt ccgccagcac atcttcgcgc cactcaagat gaccgatacg tcgtacaacg
     3721 tgccgcagga gaaggcgccg cgcctggtgg ctgcgcagca gcgcgccggg gaacgcatgg
     3781 atggtgcggt agtgctgcag tcgccgcagc cggggctgac catcgccgcg ccgatcggcg
     3841 gcggcggcct cgcctcgacc gccggcgatt acggacgctt catgcgcatg ttactcaatg
     3901 gcggcgcgct cgacggcgcc cgcgtcctca aggcccagac agttgccctt atgggccaga
     3961 accacatcgg cgcgattgga gtcccggcgc tcaaaagcgc gctgccgcgc agcgctgatt
     4021 tcaacttcat cgcggacggc cgcgacaagt tcggattggg cttcctgatc acgaccgacc
     4081 aggtgcccgg caagcgctcg ccgggcagcc tgagctgggg cggcatcaac aacacctatt
     4141 tctgggtcga cccgagccgc ggcatcggcg gagtcatcat gatgcagtac ctgccgttcg
     4201 ccgaccccaa ggcgctggct ctacacgaag cattcgagcg cgccgcatat cggcttgcgg
     4261 cggctcagcc aacggcggcg cgcgatgagc gcctctctcc tgtcgcctta gcgaagtgaa
     4321 agaagggtgc gggtgaagga gaccgcgccg atgatcatcg attcgcagat ccacgcctat
     4381 gaggcgaata cgccgaagcg gccgtggcgc acggtaccga actggcctcc cagcgccaac
     4441 ggcgacgagc aggtggcggc gtttgacaag ctcgggatag acggcgcgat cttcatt
//
LOCUS       DASPZD010000311         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1136117, whole genome shotgun sequence.
ACCESSION   DASPZD010000311 DASPZD010000000
VERSION     DASPZD010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136117"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1048
                     /locus_tag="VEM33_06055"
     CDS             179..1048
                     /locus_tag="VEM33_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HYS51421.1"
                     /translation="MLNSRVLCALLTLSPVQAALAWGPEGHRMVGEIAARYTGPKANA
                     EIVELLKNDRLADGQPSGRRTLGEVANWADEIKDTEWGKRRGSWHYDDVPLCEAGEYS
                     KYCKNGRCASAQLARQIEILGNERAKPGQRNEALKWVVHLMGDLHQPLHAANRGDRGG
                     NRVRVSFFGERDNPPYGTLTLHAVWDVSMVRRLISDRGGERAIVSPPITDGNRNAWEK
                     GSISDWTAESHRIARDTVYPLLPVAGSCSSRIVEVVAIDQAYYAKAAPVIEAQIRRAG
                     VRLARVLNETLGR"
     gene            1081..1527
                     /locus_tag="VEM33_06060"
     CDS             1081..1527
                     /locus_tag="VEM33_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51422.1"
                     /translation="MADSTLLEQYKALIGDVGNIGTRYATANGFYLSIVSALLGVVAF
                     TESGKPLAQVDLVLVLTVAAFAIVVCHVWIKTLAFYRGLFGVKLQLLGEMEKELAFPA
                     FSREHELMKERGVRPLIDNEIWVPRVLWVFFAVIAATALALIFVDG"
     gene            1702..2922
                     /locus_tag="VEM33_06065"
     CDS             1702..2922
                     /locus_tag="VEM33_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYS51423.1"
                     /translation="MKSDKRPQSGPAVPDGDALFGSLFAQTAVGVAVGSLDGRFLRVN
                     EALCRLLGYSERELVEKSFEEITHADDRQASRDFQQELVTTSQSRVYEKRYVHKSGST
                     VWVRITGTLVRDRSGSPLCSVALIHDITELKLTEEALKASESRFRSMVEMSSDWYWVQ
                     DENFRFVELPGVEKRGIDPEAFIGKARWELQGPGSLPEKSWQQHREKLERHEPFSDFV
                     YTVPDNAGEMRYLSVTGEPIFDAQGKFKGYHGVGKDVTEKARGLKDLEDSEERYRMLF
                     DIHPRPMWVVENKTLRFLAVNQAAVDHYGYSREEFLSMTAEQLRPPEDIAQLLKDFQD
                     PSRSYMQRVARHRKKSGEQINVEIVSFNLAFDGRPARLAVISDVTERLKAEQQSRELE
                     ARYQALIASRKDPG"
     gene            2931..>3130
                     /locus_tag="VEM33_06070"
     CDS             2931..>3130
                     /locus_tag="VEM33_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYS51424.1"
                     /translation="MSAKAKPPYRADHVGSLLRPPEIKKARAEIERGEMSAGELGAIE
                     DRCIREAVARQESIGLQAVTDG"
BASE COUNT          601 a          953 c         1056 g          520 t
ORIGIN      
        1 gaggtcaacc gcgcgatgaa gctgcccgac gtgagcgaca agctcgtgac cgccggcctc
       61 atcgtcgtcg ccgaatcgcc ggaatacttc ggcgaggtca tccgcggcga ttatgccaag
      121 tacggcaagc tcgtgcgcga catcggattt caaccgcagt gacgggtgtc tggatctctt
      181 gctgaattcg cgcgttcttt gcgcgctcct caccctttcg ccggtgcaag ccgctcttgc
      241 ctggggcccg gagggacatc gcatggtggg cgaaatcgcg gcccgctaca ccgggccgaa
      301 ggccaatgca gagatcgtgg aactcctgaa gaacgaccgc ctcgccgacg gccagccatc
      361 cggccggcgc actctcggcg aagtcgcgaa ctgggccgac gaaatcaaag acacggaatg
      421 gggaaagcgg cgcggttcct ggcattacga cgacgttccg ctgtgcgagg caggggagta
      481 ctcgaagtac tgcaagaacg gccgatgcgc ttccgcgcag ctcgcccgtc aaatcgagat
      541 cctcggcaac gagcgggcga agcccgggca gcgcaacgag gccctcaagt gggtggtgca
      601 tctgatgggc gacctccacc aaccgctgca cgccgccaac cggggcgacc gcggcgggaa
      661 ccgggtgcgg gtgtccttct tcggggaacg cgacaacccg ccctacggca ccctcacgct
      721 gcacgccgtc tgggacgtga gcatggtgcg caggctgatc tcggaccgcg gcggcgagcg
      781 cgccatcgtt tccccgccga tcacggacgg gaacaggaat gcgtgggaaa agggctcgat
      841 ttccgattgg acagccgaga gccaccggat cgcgagagac accgtctatc cgctgcttcc
      901 ggtggcgggc tcgtgctcga gcaggatcgt ggaggtggtc gcgatcgacc aggcctacta
      961 cgcgaaggcg gctcccgtga tcgaggccca gatcagaaga gccggcgttc ggctcgcgcg
     1021 ggtgctgaac gaaacgctgg gccggtgacg ctgcgaaact caatcgaggg gagacaaacg
     1081 atggcagatt cgacgctgtt ggaacagtac aaggctctga tcggcgatgt gggaaacatc
     1141 ggcacgcgct acgcgacggc gaacgggttc tacctctcca tcgtctcggc gctgctcggc
     1201 gtggtcgcgt ttaccgagtc ggggaagcct cttgcccagg tcgatctggt tctcgtgctg
     1261 accgtcgcgg ctttcgccat cgtcgtctgt cacgtgtgga tcaagaccct ggccttttac
     1321 cgagggcttt tcggcgtgaa gctgcaattg ctcggcgaga tggagaagga gctggcgttc
     1381 ccggctttct cgcgagagca cgagctcatg aaggaacgcg gcgtccggcc gctgatcgac
     1441 aacgagatat gggttccgcg cgtcctctgg gtgttcttcg cggtgatcgc cgcaacggcc
     1501 ctggcgttga ttttcgtgga cggctgagcg gtggactcag tactcgagcg ggattttcgc
     1561 gttccgaatc accttcgcgt aggtcgcaag atcgaggtgg aggggtgaga agccctgacg
     1621 gcacccgggc aggcgcggtt tcgttatata gttcatacgc acgagactct acattgctcg
     1681 gtatcgggga ggtccgaggc catgaaatcg gataaacgtc cacagagcgg gccagccgtt
     1741 ccagacggcg atgccttgtt cggctcgctc ttcgcgcaga ccgcggtcgg cgtcgcggtc
     1801 gggtcgctcg acggccgctt tctgcgcgtc aacgaagcgt tgtgccgcct gctcggctac
     1861 agcgagcgag agctggtgga aaaaagcttt gaagagatca cccacgccga cgatcggcag
     1921 gcgtccaggg acttccagca ggagctcgtc acgacgtccc agtcgcgcgt ctatgaaaag
     1981 cgctacgtgc acaagagcgg ctcgacggtc tgggtgcgga tcacggggac cctcgtgcgg
     2041 gaccgctcgg gctcgcccct gtgctcggtc gcgctgattc acgacatcac cgagctcaag
     2101 ctcaccgagg aagcgctgaa ggcgagtgaa tcgcggttcc gcagcatggt cgagatgagc
     2161 tcggactggt actgggtgca ggacgagaat tttcgcttcg tcgagctgcc cggcgtggag
     2221 aagcggggga tcgatcccga agcattcatc ggcaaggcgc gctgggagct tcagggtccc
     2281 gggtcgcttc cggaaaagtc ctggcagcag caccgcgaaa agctcgagcg tcacgagccg
     2341 ttcagcgact tcgtatacac ggttcccgac aacgccggcg aaatgcgcta tctgagcgtg
     2401 accggcgagc cgattttcga cgcgcagggc aaattcaagg ggtaccacgg cgtcggaaag
     2461 gacgtcaccg aaaaggcgcg cgggctgaag gacctggaag acagcgaaga gcgctaccgg
     2521 atgctgttcg acatccatcc ccgtccgatg tgggtcgtgg aaaacaagac cttgcgtttc
     2581 ctcgccgtca accaggcggc cgtcgaccac tacggctact cgagggagga gtttctgtcc
     2641 atgaccgccg agcagcttcg cccgccggag gacatcgcgc agctgctcaa ggactttcag
     2701 gatccgagcc gttcctacat gcagcgcgtg gcgcggcacc gcaagaagag cggggagcag
     2761 atcaacgtcg aaatcgtctc cttcaatctc gcgttcgacg gccggccggc gcgcctggcc
     2821 gtgatcagcg acgtcaccga gcgcctgaaa gcggagcagc agtcacgcga gctcgaggca
     2881 cggtaccagg cgctgatcgc gtcacgaaaa gaccccggct agcgccggcg atgagcgcga
     2941 aagcaaagcc gccctaccgg gcagatcacg tcggcagcct cctgcggccg cccgagatca
     3001 agaaagcgcg cgccgagatc gagcgcggcg aaatgagcgc tggggagctg ggcgcgatcg
     3061 aagaccgctg catccgcgag gcggtcgcgc gccaggaatc gatcgggttg caggccgtga
     3121 ccgacggcga
//
LOCUS       DASPZD010000312         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_202856, whole genome shotgun sequence.
ACCESSION   DASPZD010000312 DASPZD010000000
VERSION     DASPZD010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202856"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VEM33_06075"
     CDS             <1..365
                     /locus_tag="VEM33_06075"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYS51425.1"
                     /translation="LLGIVALDLRLKNLNRTWERILGYPRAMLLGKPLTRLIDQGEQA
                     PALMLVNTRLVAEAKPIEFSLRCSDGTYRCFEWERRPARGEEGMFITGRDITERKKME
                     TTANLARYMQAKKAERSA"
     gene            complement(376..>3105)
                     /gene="uvrA"
                     /locus_tag="VEM33_06080"
     CDS             complement(376..>3105)
                     /gene="uvrA"
                     /locus_tag="VEM33_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYS51426.1"
                     /translation="LGAAERGVPLQARISQSIRIHNAREHNLKSIDVEIPRNEFTVVT
                     GVSGSGKSTLAFDILFAEGQRRYLESLNAYARQFVQGAARPDVDAIFGIPPTVAIEQR
                     TSRGGSKSTVATLTEIYHFLRLLYVKLGRQYCPDCNVPIEPQSQDAIAARLLREYRGQ
                     RIGLLAPLVVNRKGYYTDLAKWARGKGYEFLRVDGEFLPTAKWPRLSRFQEHTLELPV
                     ADLRVAADNESEIRRALGQALEFGKGVVHVLAGLDRLEGAKAESRSARVEMDLPQRVF
                     STKRACPSCGRSFAELDPRLFSFNSKHGWCPGCYGTGLKLDKVEWDEERQKTGTEDHV
                     LDSWIEWLEVDESCPACEGKRLNREALAVRYQGRSISELAALPVGKLEALFRSLELSG
                     REAEIARDIVAELRSRLSFLDQVGLSYLSLDRSAPTLSGGEAQRIRLASQLGSNLRGV
                     CYILDEPTIGLHPRDNKILLDTLEQLQAKGNTLVVVEHDEDTIRRASHVIDLGPGAGK
                     QGGRVIAEGSADDLMRNADSLTGRFLRNPLAHPLHPLRQVVRSTPSIEIAGASLHNLK
                     NIDLRVPLARVSVITGVSGSGKSTLARDVLYTNLSRMVGERRQNKPAPLSGCRAIRGA
                     ENVGRVLEVDQTPIGKTPRSCPATYVGFWDAIRRLFAEANESRMRGWSASRFSFNTKG
                     GRCEACEGQGIKKIEMSFLPDVKVTCEVCGGARFNPETLTVRWKGKSIGEVLAMSVDE
                     AVDFFAAHASTHHALRLLQDVGLGYLTLGQQSPTLSGGEAQRIKLVTELSKVRTDAQG
                     NAASRPGRGGADNKTLYVLDEPTVGLHMADVEKLLRVLHRLVDAGNTVVVIEHNLDVI
                     AEADWIVDLGPEGGDAGGCIVAQGSPEALVAPRETGHTAKVLRNFLKDRRLAA"
BASE COUNT          510 a         1007 c         1053 g          535 t
ORIGIN      
        1 tcctgctcgg gatcgtcgcg ctcgatctga ggctcaagaa cctgaatcga acgtgggaaa
       61 ggatcctcgg gtacccccgc gcgatgctgt tgggaaaacc gctcacgagg ctcatcgatc
      121 agggcgaaca ggccccggcg ctgatgctcg tgaatacgcg cctggtcgcc gaagcgaaac
      181 cgatcgaatt ctcgctgagg tgcagcgacg gaacgtaccg gtgcttcgag tgggagcgcc
      241 gcccggcgcg cggagaagag ggcatgttca tcaccgggcg ggacatcacc gagcgcaaga
      301 aaatggaaac gacggccaac ctggcccggt acatgcaggc gaagaaagcg gagcgctcgg
      361 cctgacgccg cgtctctagg ccgccaagcg gcggtctttc agaaaattcc tcagtacctt
      421 cgcggtgtgc cccgtctccc gcggcgcgac caatgcttcg ggcgagccct gcgccacgat
      481 gcatccgccc gcgtcgccgc cctccgggcc gaggtcgacg atccagtcgg cctcggcgat
      541 gacatcgaga ttgtgctcga tcaccaccac ggtgttgccc gcgtccacga ggcggtgcag
      601 cacgcgaagg agcttctcca cgtcggccat gtgcaggccg accgtgggct cgtcgaggac
      661 atagagcgtc ttgttgtcgg cgccgccccg gccggggcgc gacgcggcat tgccttgcgc
      721 atcggtgcgc accttggaaa gttcggtgac gagcttgatg cgctgcgcct cgccgccgga
      781 gagcgtcgga ctctgctggc cgagggtcag gtagcccagg cctacgtcct gcagcaagcg
      841 cagcgcgtga tgggtcgagg catgcgcggc aaaaaagtcg accgcctcgt ccacgctcat
      901 cgccagcact tcgccgatcg attttccctt ccagcgcacc gtgagcgttt cggggttgaa
      961 gcgcgcacct ccgcagacct cgcaggtcac tttgacgtcc ggcaggaagc tcatctcgat
     1021 tttcttgatg ccctggcctt cgcaggcctc gcagcgcccg cccttggtgt tgaacgaaaa
     1081 gcggctcgcg ctccaaccgc gcatgcgcga ttcgttggcc tcggcgaaca ggcgccggat
     1141 cgcgtcccag aaaccgacgt aagtcgcggg gcaggagcgc ggcgtcttgc cgatcggcgt
     1201 ctggtcgact tcgagcacgc ggccgacgtt ctccgcgccg cggattgctc ggcaaccgga
     1261 cagcggcgca ggtttgttct gcctgcgctc acccaccatt ctcgaaaggt tcgtgtagag
     1321 cacgtcgcga gccagcgtcg atttgcccga gcccgagacg ccggtgatca cgctgactct
     1381 cgcgaggggc acgcgcaggt cgatgttttt caggttgtgc agcgacgcgc ccgcgatttc
     1441 gatagagggc gtgctgcgca ccacctgtcg caggggatgc agcggatgcg cgagcggatt
     1501 cctgagaaac cgccccgtga gggaatcggc gttccgcatc aagtcgtcgg cggagccttc
     1561 ggcgatgacg cggccgccct gcttaccggc gcccgggccg agatcgatca cgtgcgaggc
     1621 gcgccggatg gtgtcctcgt cgtgctcgac caccaccagc gtgttgcctt tcgcctgaag
     1681 ttgctccagc gtgtcgagca ggatcttgtt gtcgcgcggg tgcaacccga tagtgggctc
     1741 gtccaggatg tagcacacgc cgcgcaggtt ggatccgagc tgcgaggcga ggcggatgcg
     1801 ctgcgcctcg cctccggaga gcgtcggcgc cgagcggtcg agcgaaagat aggagaggcc
     1861 cacctgatcg aggaacgaga gacgcgaacg cagttccgcg acgatgtcgc gcgcgatctc
     1921 ggcctcgcgg cctgagagct ccaggcttcg gaacagcgcc tcgagcttgc ccacggggag
     1981 cgcggcgagc tcggagatcg agcgcccctg atagcgcacc gcgagcgcct cgcggttgag
     2041 acgcttgccc tcgcaggcgg ggcaactctc gtcgacctcc agccattcga tccaggagtc
     2101 gagcacatgg tcctcggtcc cggtcttctg gcgctcctcg tcccattcca ccttgtcgag
     2161 cttcaggccc gtgccgtagc agccgggaca ccagccgtgc ttggagttga acgagaaaag
     2221 ccgcgggtcg agctcggcga agctgcgccc gcacgagggg caggcgcgtt tcgtcgagaa
     2281 gacccgctgc gggaggtcca tttccactcg tgccgaccgg gattcggcct tggccccctc
     2341 cagacgatcg agtcccgcga gcacatgcac gacgcctttg ccgaattcca gggcctggcc
     2401 gagcgcccgg cggatctcgg actcgttgtc ggcggcgacg cgcaggtcgg cgaccgggag
     2461 ttccagcgtg tgttcctgga atcgcgacag ccgcggccat ttggccgtgg gcaggaattc
     2521 gccgtcgacg cgtaggaatt cgtagccctt tccgcgtgcc cacttggcga ggtcggtgta
     2581 gtagcccttc ctgttgacga cgagcggggc gagcaggccg atgcgctgtc ccctgtactc
     2641 gcgcaataga cgcgccgcga tggcgtcctg gctctgcggc tcgatcggca cattgcaatc
     2701 ggggcagtac tgcctgccga gcttgacgta gagcaagcgg aggaagtgat agatctcggt
     2761 gagcgtcgca acggtgctct tgctgccgcc gcggctggtg cgctgctcga tcgcgaccgt
     2821 cggcgggatg ccgaagatcg cgtcgacgtc gggccgcgcg gctccttgca cgaactggcg
     2881 cgcgtaggcg ttgagcgact ccagataccg ccgctggcct tcggcgaaca ggatgtcgaa
     2941 agcgagcgtg gattttccgg agcccgacac gccggtcacg acggtgaact cgttgcgcgg
     3001 gatctcgacg tcaatggatt tcaggttgtg ctcgcgcgcg ttgtggatcc gaatcgactg
     3061 gctgatacgg gcctgaagcg gcacgccgcg ctcggccgca ccaag
//
LOCUS       DASPZD010000313         5607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1239057, whole genome shotgun sequence.
ACCESSION   DASPZD010000313 DASPZD010000000
VERSION     DASPZD010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239057"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1871)
                     /locus_tag="VEM33_06085"
     CDS             complement(72..1871)
                     /locus_tag="VEM33_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS51427.1"
                     /translation="MLGPNSQLHSRAAALRLFAAMAFVVFAGCAATPPQQAAAGEEAQ
                     DESDADSPAGEAQALAPEPVDRASLPNQELTDNLLYEFLLAEIAGQRGNTTLSAQAYV
                     DLAKRTRDPRIARRATEVALYARMNNAAIESAKIWHETEPGSTRPLQALAGLLVAAGR
                     YDEALPNLRKLLAGSANDPANGFTQLTRTLANAQDKQAALKLVHSLAADYPKLAESHL
                     AVARAAVAAGDERLALEEARKAGQLRPEWEAPVLLEAQLLQKTSADQTASFLAAYLKK
                     YPAARETRLAYARVLVAQKRFGEARAEFQKLMAGAPDSTDVAFAVALLSLQLKDYDAA
                     EKYLRSLIESPYRDKDGVRLYLGQVAEERKNLPEALRWYAEIGEGEQYVQAQIRYAQV
                     LAKQGKLDEARGRLQEAAAKNSQQRVQLILAEAQLLRDANQPKAAFDLVGRALDRVPN
                     NPELLYDYAMLAEKIERVDILEASLRKLIEIRPEHAHAYNALGYSLADRNQRLPEARE
                     LIEKALQLAPEDSFIIDSMGWVLYRMGNLKDSLGYLRRAFAGRPDPEIAAHLGEVLWA
                     LGERAEAERVWGEATRETPDNETLVNTIKRFKR"
     gene            1943..2758
                     /gene="mutM"
                     /locus_tag="VEM33_06090"
     CDS             1943..2758
                     /gene="mutM"
                     /locus_tag="VEM33_06090"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HYS51428.1"
                     /translation="MPELPEVEITRRGIEPFVLGKTITGVAVRNPNLRQRVPRKLART
                     LVGQRVKRLERRGKYLLFECDTGSLIVHLGMSGSLRVTEPGTVPREHDHVDLVFGGIV
                     LRLRDPRRFGVVLWKDGDVFRHPLLARLGVEPLGEHFSPEFLYRATRGRDVGIKQLLM
                     NANVMVGVGNIYANESLFRAGIHPRARSGSLSRARCERLAAAVRDVLLAAIAAGGSSL
                     RDFVRSDGSPGYFQQHYYVYDRAALPCRVCGAAIRVSRTGQRSSFFCTTCQKK"
     gene            complement(2765..3253)
                     /gene="coaD"
                     /locus_tag="VEM33_06095"
     CDS             complement(2765..3253)
                     /gene="coaD"
                     /locus_tag="VEM33_06095"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYS51429.1"
                     /translation="MIKAVYPGTFDPITRGHEDLVRRAAALLGEVVVGIAASQTKRPF
                     FTLEERVDLARDVLKSYRNVKVEGFRGLLMDFVRGQKAQVVLRGLRAVSDFEYEFQMA
                     GMNRNLYPDVETIFLTPEEQYQFISASIVREIAALGGDVSKFVQPRVARALAAKVGKG
                     RK"
     gene            complement(3222..3803)
                     /gene="rsmD"
                     /locus_tag="VEM33_06100"
     CDS             complement(3222..3803)
                     /gene="rsmD"
                     /locus_tag="VEM33_06100"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HYS51430.1"
                     /translation="MRRTRPNTVRIIGGLWRSRLIEFPDAADLRPTPDRVRETLFNWL
                     GRDLTGMECLDLFAGSGALGFEALSRGAASVIMVEKDPAALRALRDNAHKLGATGLTV
                     VRGDALEFVRGARSRFDLVFVDPPYRLGMQVAALELLRGLLAEGGRVYVESDGAFEAP
                     RGWAVFRRARAGNVHFHLLAREADDQSGVSRNV"
     gene            complement(3790..5085)
                     /locus_tag="VEM33_06105"
     CDS             complement(3790..5085)
                     /locus_tag="VEM33_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYS51431.1"
                     /translation="MRRAPLWVLMTLALAAPAEAGVKIDHWTAQSGARVSFVESHALP
                     IVDVAVEFAAGSAYDSREQAGLARLTLAMLKAGSSRYSETEASRRIADAGAQLLQNFD
                     LDRAGFALRSLSSEAERKAATQTLADMLQAPLFPSEAFEREKASAIANAREAETLPGE
                     VAERRLYALMYPAHPYGFSETPDTLASIKREDVERHYRSSYGAARAVVTIVGDLDRDA
                     ARALAEELTSRLSKGEGSALQPVAAAAAGATLRVAHPSTQSHVLLGVAALTRADPDYF
                     PLFVGNYSLGGGGFTSRLLKEVREKRGYAYSAYSHFLPYEREGPFLVGLETRRDQARE
                     ALELARAVVGEFVKHGPTEAELSAAKKSLVGGFPLRIDTNRKILDQVAVIGFYRLPLA
                     WLDEFPARVEGVTLAQVRSAFARRIDPEKLSIVVVGAPD"
     gene            complement(5088..>5607)
                     /locus_tag="VEM33_06110"
     CDS             complement(5088..>5607)
                     /locus_tag="VEM33_06110"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HYS51432.1"
                     /translation="FAISLLAELLGGSDAARLHQVLVRERRIATSVSADYEMISRGPG
                     MLFVEAAAAQGRSGAELEAGVREVISRIAADGVTAEELNRARVQLLAQLVYKRDSFFA
                     QALELGELESSGLPFRAADRIPERLKAVTAEQIQAAAARYLLDDGLTVAVLDPQPLNP
                     RAASAAAPGTKR"
BASE COUNT          890 a         1885 c         1877 g          955 t
ORIGIN      
        1 gagggaagcg ttcctctgca acctccaatt tgggggatct aaagggggcg tgcccccttt
       61 tccaaaacct atcagcgctt gaagcgcttg atcgtattga cgagtgtttc gttgtccggg
      121 gtttcccggg tcgcttcgcc ccagacgcgc tcggcttcgg cgcgctcgcc cagcgcccag
      181 agcacttcgc ccaggtgagc ggcgatttcc gggtcgggcc gcccggcgaa cgcgcggcgc
      241 aggtagccga gcgagtcctt caggttgccc atccggtaca gcacccatcc catgctgtcg
      301 atgatgaacg aatcctcggg agcgagctgc agcgcctttt cgatcagctc acgcgcctcg
      361 ggcaggcgct gattgcggtc ggcaagggaa taaccgagcg cgttgtacgc gtgcgcatgc
      421 tcgggccgta tctcgatcag cttgcgcagg gaagcctcga ggatatccac tcgctcgatc
      481 ttctccgcga gcatggcgta gtcgtagagc agctcgggat tgttcggaac gcgatcaagc
      541 gcccgcccga ccagatcgaa cgcggccttc ggctggttgg cgtcgcgcag gagctgcgct
      601 tcggcgagga tcagctgcac ccgctgctgg ctgttcttcg cggccgcttc ctgcagccgc
      661 ccccgtgcct cgtcgagctt gccttgcttg gcgagcactt gcgcgtagcg gatctgcgcc
      721 tgcacatatt gctcgccttc gccgatctcg gcgtaccagc gcagcgcctc cggaagattc
      781 ttgcgctctt cggcgacctg gccgagatac aagcggaccc cgtccttgtc gcggtaggga
      841 ctctcgatca ggctcctgag atatttctcg gcagcgtcgt aatccttgag ctgcagggaa
      901 agcagcgcca cggcgaaagc cacgtcggtg ctgtcgggag cgccggccat gagcttctgg
      961 aattcggcgc gcgcctcgcc gaagcgcttt tgcgccacca gcacgcgcgc ataggcgagg
     1021 cgcgtctcgc gcgcggcagg atattttttc aagtaggcgg cgaggaacga tgcggtctga
     1081 tccgccgagg tcttctgcag cagctgcgct tcgagcagga caggcgcttc ccattccggc
     1141 cgcagctgcc cggccttgcg ggcttcctcg agtgcaagac gttcgtcgcc cgcggcgacc
     1201 gccgcccgcg cgacggcaag atgcgactcg gcgagcttgg gatagtctgc ggcgaggctg
     1261 tgcacgagct tcagagcggc ctgcttgtcc tgcgcattgg caagcgtgcg ggtcaactgc
     1321 gtgaatccgt tggcggggtc gttggcagag ccggccagga gcttcctgag attgggcagc
     1381 gcctcgtcgt agcggcccgc ggcgaccagc aatccggcga gcgcctgcag agggcgcgtc
     1441 gagccgggct cggtctcgtg ccagatcttt gcggactcga tcgcggcgtt gttcatccgg
     1501 gcgtagaggg cgacttcggt ggcacgccgc gcgatgcgcg gatcgcgcgt gcgcttcgca
     1561 agatcgacgt acgcctgcgc agagagcgtg gtgttgccgc gctggcccgc gatctctgcg
     1621 agcagaaact cgtagagcag gttgtcggtc agctcctgat tgggaagcga cgcgcggtct
     1681 acgggctcgg gtgcgagcgc ctgcgcttcg ccggcgggcg agtccgcgtc ggattcgtcc
     1741 tgcgcttcct ctcccgcggc ggcctgctgc ggcggagtcg cggcgcatcc cgcaaacacg
     1801 acgaacgcca tggcggcaaa cagccgcaaa gcggcggccc gactgtgcaa ctggctgttt
     1861 ggaccgagca tggaggtact tgctgtcgtg aagggctagt ttaggtatat ttcgaaaggc
     1921 cgacaaggac attgagtcgc ccgtgccaga gctgcccgaa gtcgagatca cccgccgcgg
     1981 catcgagcct ttcgtgctcg gcaagacgat cacgggcgtc gccgtgcgca atcccaacct
     2041 gcgtcaacgc gttccccgca aactcgcccg caccctcgtc ggccagcggg tgaaacgcct
     2101 ggagcggcgg ggaaagtacc tgctgttcga gtgcgacacc ggctcgctca tcgtgcacct
     2161 gggcatgtcg gggagcctgc gcgtgaccga gccgggaacc gtcccccgcg agcacgatca
     2221 tgtggacctc gtcttcggcg gcatcgtatt gcgcctgcgc gatccgcgac gcttcggcgt
     2281 ggtgctgtgg aaagacggcg acgtgttccg ccatcccctg ctcgcgcggc tcggcgtcga
     2341 gccgctcggc gagcatttct cgcccgagtt cctctatcgg gcgacgcgcg gccgcgacgt
     2401 cggcatcaaa cagctgttga tgaacgcgaa cgtcatggtg ggggtcggaa acatctacgc
     2461 gaacgaaagc ctgttcaggg cgggcatcca tccgcgcgcg cgctcaggaa gcctctcgcg
     2521 cgcacgctgc gagcggctgg ccgccgcggt gagggacgtt cttctggccg cgatagccgc
     2581 cggcggatcg agcctgcgcg acttcgtgcg ctccgacggc tccccgggat attttcagca
     2641 gcactactac gtctacgacc gcgccgccct tccgtgcagg gtctgcgggg ccgcgatccg
     2701 ggtgtcgcgg acgggccagc gctccagctt cttctgcacg acctgccaga agaagtgatg
     2761 cggcctactt ccgccccttg ccgacctttg ccgcgagagc gcgcgcgacg cgtggctgaa
     2821 cgaacttgct gacatcgccg ccgagagccg cgatctcgcg cacgatggac gcagagatga
     2881 actggtattg ctcctccggt gtcaggaata tcgtctcgac gtccggatag agattgcggt
     2941 tcattcccgc catctggaat tcgtactcga agtccgacac cgcccgcaag ccgcgcagaa
     3001 ccacctgggc cttctgtccg cgcacgaaat ccatgagcag cccccggaag ccctcgacct
     3061 tcacgttccg gtacgacttg agaacatcgc gcgcgaggtc cacgcgctcc tccagggtga
     3121 aaaacggacg cttcgtctgg ctcgcggcga tgcccaccac gacttcgccg aggagcgcag
     3181 cggcacgccg caccaggtct tcgtggcctc gcgtgatcgg atcaaacgtt ccgggataca
     3241 ccgctttgat catcggcctc cctcgccaaa agatgaaaat gcacatttcc cgcgcgcgcg
     3301 cgtctgaaaa cggcccagcc ccgaggcgct tcgaaagcgc cgtcgctttc cacgtacacc
     3361 cggccccctt cggcgagaag cccgcgcagc agttcgagcg ccgcgacctg catgcccaga
     3421 cggtacggcg gatcgacgaa taccagatcg aatcgcgagc gcgcgccgcg gacgaattct
     3481 agcgcatcgc cgcggacgac cgtcagaccg gttgcgccga gcttgtgcgc attgtcgcgc
     3541 aaggcgcgca gcgccgcggg gtccttctcg accatgatga ccgaggcggc accgcgcgag
     3601 agcgcctcga agccgagcgc gccgctgccc gcgaagaggt cgaggcactc cattccggtc
     3661 aggtcccgcc cgagccagtt gaacagggtt tcccgaacgc ggtccggggt cggtctcaag
     3721 tccgccgcat cggggaattc gatgagtcgg ctgcgccaca gaccgccgat gatccggacg
     3781 gtgttgggtc tagtccggcg caccgacgac gacgatcgag agcttttcgg gatcgatgcg
     3841 ccgcgcaaag gcgctcctca cctgcgcgag ggtcacccct tcgacgcgcg ccgggaactc
     3901 gtcgagccag gcgaggggca ggcggtagaa accgatcacg gcgacctgat cgaggatttt
     3961 gcggttggta tcgatgcgca ggggaaatcc gccgacgagg ctcttcttcg ccgcgctcag
     4021 ctcagcttcc gtcggaccgt gcttcacgaa ctcgccgacg accgcgcgcg ccagctcgag
     4081 cgcttcgcgc gcctggtcgc gccgcgtctc caatcccacc aggaacggtc cctcgcgctc
     4141 gtagggcagg aaatggctgt aggcgctgta ggcatagccg cgcttctcgc gcacttcctt
     4201 gaggagccgc gaggtgaatc cgccgcctcc cagagagtaa ttgccgacga aaaggggaaa
     4261 gtagtccggg tcggctcggg tgagcgccgc gacgccgagc agcacgtggc tctgggtgga
     4321 cgggtgtgcc acgcgcagcg tcgccccggc cgccgcggcc gcgacgggct gcagcgcgga
     4381 accttcgcct ttcgaaagac gcgaggtcag ctcctcggcg agcgccctcg cggcgtcgcg
     4441 gtccagatcg ccgacgatcg tgaccactgc gcgggcggcg ccgtagctgc tgcggtagtg
     4501 gcgctcgacg tcctcgcgct tgatcgaggc gagcgtgtcc ggggtctcgc tgaatccgta
     4561 gggatgcgcc ggatacatga gcgcatacag gcgccgctcc gcgacctcgc cgggcagcgt
     4621 ttcggcttca cgcgcgttcg cgatggcgct cgccttctcg cgctcgaagg cctcgctcgg
     4681 aaacagcggc gcctgcagca tgtcggccag agtttgcgtc gccgccttcc gctcggcctc
     4741 ggaggaaagg ctcctcagcg cgaagccggc gcgatccagg tcgaagttct gcagcagctg
     4801 cgcgcccgcg tcggcgatgc ggcggcttgc ctcggtttcg gagtaacgag acgagcccgc
     4861 cttgagcatg gcgagcgtga gccgcgcgag ccccgcctgc tcgcgcgaat cgtaggcgct
     4921 gcccgccgcg aactcgaccg cgacgtccac gatcggcagg gcatggcttt cgacgaagct
     4981 gacccgcgct cccgattgcg cggtccagtg atcgattttc actcccgcct cggccggcgc
     5041 ggcaagggcg agagtcatca ggacccagag gggcgcgcgg cgcacggtca gcgtttcgtt
     5101 ccaggagcgg cagcgctcgc cgcgcgcgga ttcaggggct gcgggtcgag cacggcgacc
     5161 gtcagtccgt cgtcgaggag gtagcgcgcc gcggcggcct ggatctgctc cgctgtgacc
     5221 gccttcaagc gctcggggat gcgatcagca gcgcggaagg ggagtccgct cgactccagt
     5281 tccccgagct cgagcgcctg cgcaaaaaac gagtcgcgct tgtagacgag ctgggcgagc
     5341 agctggaccc ttgcccggtt caactcctcg gcagtgacgc cgtcggccgc aattcgagag
     5401 atgacttcgc gcacgccggc ctcgagctcg gctccgctgc gaccctgcgc ggccgctgct
     5461 tccacgaaca gcattccagg gccgcgcgag atcatctcgt agtccgcgct cacggaagtg
     5521 gcgatgcgcc gctctcgcac caggacctgg tgcaggcgcg ccgcgtcgct tccgccgagc
     5581 agctctgcga gaagcgaaat ggcgaac
//
LOCUS       DASPZD010000314         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1720031, whole genome shotgun sequence.
ACCESSION   DASPZD010000314 DASPZD010000000
VERSION     DASPZD010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720031"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VEM33_06115"
     CDS             <1..690
                     /locus_tag="VEM33_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS51433.1"
                     /translation="ALLARVTAPPHKVSLLRRLQGASVGQRIDWIGTSMGGLIGMMLA
                     ARRDSPIRRLVLNDVGPMVPWNALARLKLTAMGVGRRFASLEEVERHLRDTCKHFGPL
                     EDRQWRRLAEHDSRRGENGAYTLALDPAVVNSMRRGGNAGVEFGNDFLFGIDLWPTWD
                     QVECPTLVLRGAESDLLLESTVRRMQVGGPRVEFVEFPGVGHAPWLMSEEQIAPVRDF
                     ILAPEPRKGAA"
     gene            687..1658
                     /locus_tag="VEM33_06120"
     CDS             687..1658
                     /locus_tag="VEM33_06120"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HYS51434.1"
                     /translation="MRICVVGAGAVGGMIGGWFAKAGHEVSLVARGAHLEAIRRRGLT
                     LISGGKPEVHPVRASSEPADFGVQDAAFICLKTHSIAAMLPRLKTLVGADTMVIPAIN
                     GLPWWYFFREGGRFDGQPVACLDPAGTLFEALAPRHILGCVVHAAAEVPEPGVVRHTA
                     GRLCLVGEPDRTRSARAERLVAAMNAAGFEARQAEDIRVEIWVKLIGNLSYNPVAALA
                     LARMNDIHGSEALLALIRAMMGEAMRVAEAYGVRIPMTIDERIDIARQLAGAKISMHQ
                     DVEKRRPLEIDAIVGAVVELARKAGIATPMIDAVYALIAERAKHLEG"
     gene            complement(1669..>2678)
                     /locus_tag="VEM33_06125"
     CDS             complement(1669..>2678)
                     /locus_tag="VEM33_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HYS51435.1"
                     /translation="NGERTVAAWLLVCCALVFAMVVVGGVTRLTHSGLSIVEWQPIVG
                     ALPPLDEAGWQEAFGKYKQTPEYRLVNPHMTLEGFKSIFWWEYVHRLLGRLIGAAFLL
                     PLLWFALRGKIARGLTWKFAVIFGLGALQGALGWYMVKSGLVDNPRVSQYRLTAHLGL
                     AFLIYAAILWVALDLLFPRTQPGAVSAGLRRFAAALAVLVFVMALSGGLVAGTRAGLA
                     YNTFPLMNGRLVPPEIFSLDPWYLNFFSNMTTVQFDHRLIAWLLAFLVAWFWLRVRRE
                     AAPRRAKLAADLLLGALALQIALGIATLLLAVPVPLAAAHQAGALLVFSAALFAAHLL
                     R"
BASE COUNT          482 a          879 c          926 g          391 t
ORIGIN      
        1 gccctgctcg cccgggtgac cgcgccgcct cacaaggtct cccttctgcg ccggctccag
       61 ggagcgagcg taggccagag aatcgactgg atcggaacct cgatgggcgg cctgatcggg
      121 atgatgcttg ccgcgaggcg cgattctccg atccggcgcc tggtgctgaa cgacgtcggg
      181 cccatggtgc cctggaatgc gctcgcgcgc ctgaagctca ccgccatggg ggtcggcagg
      241 cgattcgcga gcctggaaga ggtcgaacgc catttgcgcg atacctgcaa gcatttcgga
      301 ccgctcgaag accggcagtg gcgccgcctc gccgagcacg actcccggcg cggagagaat
      361 ggcgcctata cgctggctct cgaccctgcg gtcgtcaaca gcatgcgccg cggcgggaat
      421 gccggcgttg aattcggcaa cgattttctc ttcggcatcg acctgtggcc gacctgggac
      481 caggtcgaat gtccgacgct ggtgctgcgg ggagcggagt cggatctcct gctcgaatcg
      541 accgtcaggc ggatgcaggt gggcggtccg cgcgtcgaat tcgtggaatt ccccggggtg
      601 ggacacgcgc cgtggttgat gagcgaagag cagatcgctc cggttcgcga cttcatcctc
      661 gcgcccgaac cccggaaagg ggccgcatga ggatttgcgt cgtcggcgcc ggtgccgtgg
      721 gcgggatgat cggaggatgg tttgcgaagg ccgggcacga ggtgtcactc gtcgcgcgcg
      781 gtgctcatct cgaggcgata cgcaggcggg gactgacgct gatttccgga ggcaagccgg
      841 aagtccatcc ggtacgcgcg tcgtcggaac ccgccgattt cggcgtccag gacgcggcgt
      901 tcatctgcct caagacgcat tcgatagccg cgatgctgcc ccggctgaag acgctcgtgg
      961 gtgccgacac gatggtgatc ccggcgatca acggcctgcc ctggtggtat tttttcaggg
     1021 aaggcgggcg cttcgacggc cagcccgtcg cctgcctcga tcccgcgggc acgctcttcg
     1081 aggcgctagc cccgagacac atcctcggat gcgtggtgca cgccgccgcg gaggtccccg
     1141 agcccggggt cgtgcgccac accgccggcc ggctctgtct ggtgggcgag cccgatcgca
     1201 cgagatcggc gcgcgcggag cgcctcgtgg cggcgatgaa cgccgccggc ttcgaggcga
     1261 ggcaggccga ggacatccgg gtcgagatct gggtcaagct gatcgggaac ctctcctaca
     1321 atcccgtcgc cgcgctcgcg ctagcccgca tgaacgacat ccacggaagc gaagcgctgc
     1381 tcgcgctgat ccgcgcgatg atgggagagg cgatgcgcgt cgccgaggcc tacggcgtgc
     1441 gcataccgat gacgatcgac gagcgcatcg acatcgcaag gcagcttgcc ggcgccaaga
     1501 tctcgatgca tcaggatgtc gagaagcgcc ggccgctcga gatcgatgcc atcgtcggag
     1561 cggtggtgga gcttgcccgc aaggccggaa tcgcgacgcc gatgatcgat gccgtctacg
     1621 cgctcatcgc cgagcgggcg aagcacctcg agggataagg gtcgcgggtc agcgcagcaa
     1681 gtgcgcggcg aacagcgcgg cgctgaaaac cagcagcgcg cccgcctgat gcgccgcggc
     1741 gaggggaacc gggacggcga gcaacagggt cgcgattccc agggcgatct gcagtgcaag
     1801 cgcgccgagc agcagatcgg ccgccagctt cgcgcgccgc ggcgccgcct cgcggcggac
     1861 ccgcagccag aaccaggcca cgaggaacgc gagcagccag gcgatcagcc ggtgatcgaa
     1921 ctgcacggtc gtcatattgc tgaagaagtt caggtaccac gggtcgaggg aaaaaatttc
     1981 cggcgggacg agacggccgt tcatgagcgg gaacgtgttg tacgcgagtc ccgcccgggt
     2041 tcccgcgacc aggccgcccg agagcgccat cacgaagacc agcaccgcga gcgccgcggc
     2101 gaagcggcgc agccccgcag agaccgcgcc cggttgcgtg cggggaaaaa ggagatcaag
     2161 cgcaacccac agtatcgccg cgtagatcag gaaggcaagc ccgaggtgcg ccgtgaggcg
     2221 gtactgcgat acgcgcgggt tgtcgaccag gcccgacttc accatgtacc agcccagcgc
     2281 accctgcaac gcgcccagcc cgaatatcac cgcaaatttc cacgtcagcc cgcgggcgat
     2341 ttttcctcgc agcgcaaacc acaggagcgg cagcaggaat gccgcgccga tcagcctgcc
     2401 caggagccgg tggacatact cccaccaaaa aatgctcttg aatccctcga gagtcatgtg
     2461 cggattgacc agccgatact cgggggtctg cttgtacttc ccgaaggctt cctgccaccc
     2521 ggcctcgtcg agcggaggca gggcaccgac gatcggctgc cactcgacga tcgacagccc
     2581 cgagtgggtg agccgtgtga cgccgccgac caccaccatc gcgaacacca gcgcgcagca
     2641 gacgagcagc caggcggcga cggtgcgctc gccgtttc
//
LOCUS       DASPZD010000315         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_178567, whole genome shotgun sequence.
ACCESSION   DASPZD010000315 DASPZD010000000
VERSION     DASPZD010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178567"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..581
                     /gene="rplE"
                     /locus_tag="VEM33_06130"
     CDS             90..581
                     /gene="rplE"
                     /locus_tag="VEM33_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HYS51436.1"
                     /translation="MKQFGYKTSMQVPRIKKITLNMGVGETTTDKKILDNAVADLTKI
                     AGQRPVVTLAKKSIATFKVRKGFPVGCMVTLRGKRMYEFLDRLVTVAIPRIRDFRGIS
                     SRAFDGRGNYSLGVKEQIIFPEIEYDKIDVLRGLNISITTTAKTDDEARALLAAFHFP
                     FKT"
     gene            591..896
                     /gene="rpsN"
                     /locus_tag="VEM33_06135"
     CDS             591..896
                     /gene="rpsN"
                     /locus_tag="VEM33_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HYS51437.1"
                     /translation="MAKLALINRDKKRRKTVAKFAARRAALLAIINDARRSDEERQEA
                     REKQQQLPRNASPVRLRNRCRLTGRPRGVFRKFGLGRNKLREIALRGEIPGLIKASW"
     gene            910..1305
                     /gene="rpsH"
                     /locus_tag="VEM33_06140"
     CDS             910..1305
                     /gene="rpsH"
                     /locus_tag="VEM33_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HYS51438.1"
                     /translation="MSMTDPIADMLTRIRNAQMVGKTAVGVPSSKLKISIAQVLKDEG
                     YIDGFTVREKEGKPELEIGLKYYAGRPVIEKIERVSTPGLRIYKGRDELPRIMNGLGV
                     AIVSTSKGVMTDRKARATGVGGEVLCFVA"
     gene            1315..1848
                     /gene="rplF"
                     /locus_tag="VEM33_06145"
     CDS             1315..1848
                     /gene="rplF"
                     /locus_tag="VEM33_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HYS51439.1"
                     /translation="MSRIAKYPVTLPKGVEATLATEALSIKGPLGTVSQRLRRDVSIE
                     RDGDKLVFKALDESDEANAMSGTLRALAANMVLGVTKGFEKRLTLVGVGYRAQAQGDK
                     LNLTLGFSHPVVHPMPKGVKVETPSQTEIVIKGLDKQLVGQVAAEVRAYRSPEPYKGK
                     GVRYSNERVVLKETKKK"
     gene            1861..2211
                     /gene="rplR"
                     /locus_tag="VEM33_06150"
     CDS             1861..2211
                     /gene="rplR"
                     /locus_tag="VEM33_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HYS51440.1"
                     /translation="MDKKAARLRRASQTRRKIRELAKVRLAVHRTNTHIYAQLRSVDG
                     KVLTSASTTEKEVRTAVPNGGNKAAAVAVGKRIAEKAKGLGIEAVAFDRSGYKYHGRV
                     KALADAAREAGLKF"
     gene            2279..2800
                     /gene="rpsE"
                     /locus_tag="VEM33_06155"
     CDS             2279..2800
                     /gene="rpsE"
                     /locus_tag="VEM33_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HYS51441.1"
                     /translation="MARMQPRMQGGEQRSDGLKEKMVSVNRVTKVVKGGRILGFAALT
                     VVGDGDGAVGMGKGKAREVPVAVQKALEEARRKMFRVSLKNGTLHHAVIGKHGSSKVI
                     MLPASEGTGIIAGGPMRAVFEVMGVTNVLAKCIGSTNPYNVVRATLDGLKRMNTPAQI
                     AAKRGKSIEEITS"
     gene            2812..2994
                     /gene="rpmD"
                     /locus_tag="VEM33_06160"
     CDS             2812..2994
                     /gene="rpmD"
                     /locus_tag="VEM33_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HYS51442.1"
                     /translation="MADKKIRVTLVKSTNGCKREHRATVRGLGLRRLRQTVELKDTPQ
                     VRGMINSVTYLVRVEG"
     gene            2995..>3096
                     /gene="rplO"
                     /locus_tag="VEM33_06165"
     CDS             2995..>3096
                     /gene="rplO"
                     /locus_tag="VEM33_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197941.1"
                     /note="late assembly protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HYS51443.1"
                     /translation="MELNTLKPAPGSKHVRKRVARGIGSGTGKTGGRG"
BASE COUNT          686 a          938 c         1019 g          453 t
ORIGIN      
        1 agcccgcgaa gccggccgag aaggaagccg cgcccacgca gccgccgcgc ctgcaggtgc
       61 agtatcgcga gtcgatcgtg ccggcgctga tgaagcagtt cggctacaag acctcgatgc
      121 aggtgcctcg catcaagaag atcactctca acatgggcgt gggcgagacg acgacggaca
      181 agaaaatcct ggacaacgcg gtcgccgacc tcaccaagat cgccggacag agacctgtag
      241 ttaccctggc caagaaatcg atcgcgacgt tcaaggttcg caagggattt ccggtcgggt
      301 gcatggtgac cttgcgcggc aagcgcatgt acgagttcct agatcgtctg gttaccgtgg
      361 cgatcccgcg catccgtgac ttccgcggca tctcgagccg cgcattcgac ggccgcggca
      421 actacagcct gggcgtcaag gaacagatca tcttcccgga gatcgagtac gacaagatcg
      481 acgttctgcg ggggctcaac atcagcatca ccaccaccgc gaaaacggac gacgaggcgc
      541 gcgcgctgct tgccgccttt cactttccat tcaagacctg aaggatcgtc atggccaaac
      601 tcgccctgat caaccgcgac aagaaacgcc gcaagacggt ggccaagttc gccgcccgac
      661 gcgcggcgct gctcgcaatc atcaacgacg ccagacgctc cgacgaggag cggcaggagg
      721 cgcgcgagaa gcaacaacag ctgccgagga acgcctcgcc cgtgcgcctg cgaaaccgtt
      781 gcaggcttac cggccggccg cgcggagtgt ttcgcaagtt cgggctcggg cgcaacaagc
      841 tgcgcgaaat cgcgctgcgc ggcgaaatcc caggcctcat caaggcgagc tggtaggagg
      901 gcctcccgca tgagcatgac cgatcccatt gccgacatgc tgacccgcat ccgcaacgcc
      961 cagatggtcg gaaagactgc ggtcggcgtt ccctcctcca agctcaagat ctccatcgcg
     1021 caggtgctga aggacgaggg gtacatcgac ggcttcaccg ttcgtgagaa ggaaggaaaa
     1081 ccggagctgg agatcggcct caagtactac gcggggcggc ccgtgatcga gaaaatcgag
     1141 cgggtatcca cgccggggtt gcggatctac aaggggcgcg acgagctgcc tcgcatcatg
     1201 aacgggctcg gcgtcgcgat cgtttccacc tccaagggcg tcatgaccga tcgcaaggcg
     1261 cgcgccaccg gtgtcggcgg cgaagtgctc tgcttcgtgg cttaggaatc ggccatgtca
     1321 cggatcgcga aatatcctgt aaccctgccc aagggcgtcg aggcgacgct tgcaaccgaa
     1381 gccctctcca tcaaggggcc gctcgggacg gtgtcgcagc ggctgagaag ggacgtcagc
     1441 atcgagaggg acggcgacaa gctggtattc aaggctctcg acgagtcgga cgaggcgaat
     1501 gccatgtcgg gaaccctgcg ggcgctcgcc gccaatatgg tgctcggagt gacaaagggt
     1561 ttcgagaagc ggctgacgct ggtcggggtc ggctatcgcg cgcaggcgca aggggacaaa
     1621 ctgaacctga ccctgggttt ttcgcatccc gtggtccacc ccatgcccaa aggggtaaag
     1681 gtcgagactc cttctcaaac cgaaatcgtg atcaaggggt tggacaagca gctcgtggga
     1741 caagtcgccg ccgaggtgcg cgcataccgg tcgcccgagc cgtacaaggg caagggcgtg
     1801 cgctactcca acgagcgcgt ggtcctgaaa gaaaccaaga agaagtaggg aactgcgatg
     1861 atcgacaaaa aggcggcaag gctccggcgc gcttcgcaga cgcggcgaaa aatccgtgaa
     1921 ttggcgaagg tgcggctggc tgtgcatcga accaacaccc acatatacgc ccagctgcgc
     1981 tcggtggacg gcaaagtgct gacctccgcg tccaccacgg agaaggaagt gcgcaccgcg
     2041 gtcccgaacg gcggaaacaa agcggcggca gtcgcggtag gcaagcgcat cgccgagaaa
     2101 gcgaaaggtt tgggcatcga ggccgtcgcg ttcgaccgct ccggctacaa gtaccacggc
     2161 agggtcaaag cgctggcaga cgccgcgcga gaggccggcc tcaagttcta gattggcgac
     2221 gaacggaagg tcttcctccc gaggtcccga tttaggcgag cactcaaaag gaatcgcgat
     2281 ggcgagaatg caacccagaa tgcagggcgg cgaacagcgc tcggacggcc tgaaggagaa
     2341 gatggtgtcc gtgaaccgcg tcaccaaggt cgtgaaaggc ggacggatcc tcggtttcgc
     2401 cgcgctcacc gtcgtcggag acggcgacgg cgcagtgggc atgggcaagg gtaaggcacg
     2461 cgaggtgccc gtggcggtcc agaaggccct ggaagaagcg cgccgaaaga tgttccgggt
     2521 gtccctgaag aacggcacgc tgcaccacgc cgtgatcggg aagcacggct cctccaaggt
     2581 gatcatgctg ccggcttccg aagggacggg catcattgcc ggcggcccca tgcgcgcggt
     2641 gttcgaggtg atgggcgtga ccaacgtgct cgccaagtgc atcggctcga ccaaccccta
     2701 caacgtggtt cgcgcgacgc tcgacggctt aaagaggatg aatactcccg cgcagatcgc
     2761 ggccaagcgc ggcaagtcga tcgaggaaat caccagctga gaggatgacg catggccgac
     2821 aagaaaatcc gcgtgacgct cgtcaaaagc accaacggct gcaagaggga gcatcgcgcc
     2881 accgtgcgcg gcctcgggct gcggcgcctg cgtcaaaccg tggagctcaa ggacacgccg
     2941 caagtccgcg gcatgatcaa cagcgttact tatctggtgc gcgtcgaggg ataaatggaa
     3001 ctcaatacgc tgaaacccgc tcccggctcc aagcacgtgc gcaagcgcgt tgcgcgcggc
     3061 atcgggtcgg ggacgggcaa aacgggcggc cgcggg
//
LOCUS       DASPZD010000316         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1062467, whole genome shotgun sequence.
ACCESSION   DASPZD010000316 DASPZD010000000
VERSION     DASPZD010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062467"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VEM33_06170"
     CDS             complement(<1..699)
                     /locus_tag="VEM33_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51444.1"
                     /translation="MKKQSRTLLLAALAAAICPAAAQDWPSKPVKWILSQPAGASPDI
                     TARLVADRLSKMWGQQVIVDNRPGGQNVIGAQIAAKSPADGYNYFWATTAALVSNPFT
                     FKSLPYDPERDFVPVALIGKSPMVVAVSNSVTANSLAELIALDKKEPGKLAAANEGAK
                     TFGGMMSQALNIATGMRLLQVPYNGVAPALQDTVAGRTQVVLVSSGAMTPFIKRGELR
                     PIAVTSGRRVTGMEN"
     gene            complement(710..1906)
                     /locus_tag="VEM33_06175"
     CDS             complement(710..1906)
                     /locus_tag="VEM33_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYS51445.1"
                     /translation="MPANERINHPISTAELERRWAAVRAAMAEHKIDALLMQSNNDWM
                     GGYVKYFTDVPATNGYPVTVVFPRDERMTVIAQGPMGVVREFAPPGDPVRRGTARFMG
                     MPSYASCPYTAEYDAQLAEQALERYSAATIGLLGRAAMSFALGDYLKRALLRAKFVDA
                     SDIVDRIKAVKSPEELDRIRRTAALQDKAMEETFAAVKPGMRELEVAAVAEHVGHKYG
                     SEQGLFLAGSGPVGTAAVFANRYQQNRVLREGDQFTILIENNGPGGFYTELGRTCVLG
                     RASQEMKDEFAFVLEAQKFTLERMKPGAKCSEIFAEYNTFMRKNKRPEEKRLHSHGQG
                     YDMVERPLIRHDETMTILKNTNMVCHPTYVTERTYSWCCDNFLIGDTGVAEKLHRFPQ
                     KIVELG"
     gene            complement(1908..>2487)
                     /locus_tag="VEM33_06180"
     CDS             complement(1908..>2487)
                     /locus_tag="VEM33_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYS51446.1"
                     /translation="QAKRKWDRFPVKLLKGKTVGIFGVGTIAAELAPKCKALGMKVVG
                     VSSAKRKLEGFDAMFGREELAQAVKEFDYFVLLTPFTPETKNIVDAKIFAAMKPSSFF
                     VNLARGGVVDEDALLDALKEKRIAGAALDVFAREPLPEDSPFWDMDNVIVTQHQGGFF
                     DGYPGFALPVVEENMRKYLAGDLKAMINVVKR"
BASE COUNT          386 a          830 c          789 g          482 t
ORIGIN      
        1 gttctccatt cccgtcacgc gccggccgga agtcaccgcg atcgggcgca gctcgccgcg
       61 cttgatgaaa ggcgtcatcg cccccgagct caccagcacc acctgcgtcc tgcccgcgac
      121 cgtgtcctgc aatgccggag cgacgccgtt gtacggaacc tgcaggagcc gcattccggt
      181 ggcgatgtta agcgcctggc tcatcatccc gccgaaggtc ttcgcgcctt cgttggcggc
      241 cgcgagcttg cccggctcct tcttgtcgag cgcgatcagc tcggcgagcg aattcgccgt
      301 gaccgagttg ctgaccgcga cgaccattgg cgattttccg atgagcgcaa ccggaacgaa
      361 atcgcgctcc gggtcgtagg gaagcgactt gaacgtgaag ggattggaaa cgagcgcggc
      421 ggtcgtcgcc cagaaatagt tgtagccgtc ggccggggac ttggccgcga tctgcgcgcc
      481 gatcacgttc tgcccgccgg ggcggttgtc cacgatcacc tgctgccccc acatcttgga
      541 gagccggtcg gccacaaggc gcgccgtgat gtcgggcgaa gcccccgcgg gctgggaaag
      601 gatccatttc accggcttgc tcggccagtc ctgcgcggcg gcggggcaga tcgccgcggc
      661 gagcgcggcg agcagcagtg ttcgtgattg cttcttcatg atctcctctc tatcccagct
      721 ctacgatctt ctgcggaaac ctgtgcagct tctcggccac gcccgtgtcg ccgatcagga
      781 agttgtcgca gcaccagctg taggtgcgct cggtgacgta ggtcgggtgg cacaccatgt
      841 tcgtgttctt caggatcgtc atcgtctcgt cgtgacggat caggggccgc tcgaccatgt
      901 cgtagccctg cccgtgggag tgcaggcgct tttcctcggg gcgcttgttc ttgcgcatga
      961 aggtgttgta ctcggcgaag atctcgctgc acttcgcgcc cggcttcatc cgctcgaggg
     1021 tgaacttctg ggcctccagc acgaaggcga attcgtcttt catctcctgc gacgcccgcc
     1081 cgagcacgca ggtgcgcccc agctctgtgt agaagccgcc cggcccgttg ttctcgatca
     1141 ggatggtgaa ctggtcgcct tcgcgcagaa cgcggttctg ctggtagcgg ttggcgaaga
     1201 cggcggcggt gcccacgggc cccgagcccg cgaggaagag tccctgctcg ctgccgtact
     1261 tgtggccgac gtgctcggcg accgccgcga cctcgagctc gcgcattccc ggcttcactg
     1321 cggcgaaggt ctcctccatc gccttgtcct gcagcgcggc ggtgcggcgg atgcggtcca
     1381 gctcctccgg gctcttcacc gccttgatgc ggtcgacgat gtcggaggcg tcgacgaact
     1441 tcgcgcgcag gagcgcgcgc ttcagatagt cgcccagcgc gaaggacatc gccgcgcgcc
     1501 cgagaaggcc gatggtcgcg gcggaatacc tttccaacgc ctgctcggcg agctgcgcgt
     1561 cgtactcggc ggtatacgga caggaggcgt aggagggcat ccccatgaag cgcgccgtac
     1621 cccgtctcac cggatccccc ggcggagcga actcgcgcac cacccccatc ggcccctgcg
     1681 cgatcaccgt catgcgctcg tccctcggga agacgaccgt cacgggatag ccgttcgtcg
     1741 ccggcacgtc ggtgaagtac ttgacgtagc cgcccatcca gtcgttgttc gactgcatga
     1801 ggagcgcgtc gatcttgtgc tccgccatcg ccgcgcgcac cgcggcccag cgtcgctcga
     1861 gctccgcggt cgagatgggg tggttgatgc gttcgttcgc gggcatttca gcgttttacc
     1921 acattgatca tggccttcaa gtccccggcg aggtacttgc gcatgttttc ctcgaccacg
     1981 ggaagcgcga agcccgggta gccgtcgaag aacccgccct ggtgctgggt gacgatgacg
     2041 ttgtccatgt cccagaaggg cgaatcctcg ggcaggggct ccctggcgaa cacgtcgagc
     2101 gcggcgccgg cgatcctctt ttctttcagc gcgtcgagca gcgcgtcttc gtccaccacg
     2161 ccgccgcgcg cgagattgac gaagaaggag gagggcttca tcgccgcgaa gattttcgca
     2221 tcgacgatgt ttttcgtctc gggagtgaag ggcgtgagga gcacgaagta atcgaactcc
     2281 ttcaccgcct gcgcgagctc ctcgcggccg aacattgcat cgaacccttc aagcttgcgc
     2341 ttcgccgagg agacgcccac cactttcatc ccgagcgcct tgcacttcgg agcgagttcc
     2401 gccgcgatcg tgccgacccc gaaaattccc acggttttcc ccttgagaag ctttaccgga
     2461 aagcggtccc attttcgctt cgcctgc
//
LOCUS       DASPZD010000317         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_173000, whole genome shotgun sequence.
ACCESSION   DASPZD010000317 DASPZD010000000
VERSION     DASPZD010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173000"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VEM33_06185"
     CDS             complement(<1..1164)
                     /locus_tag="VEM33_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYS51447.1"
                     /translation="MRSKILTGAVGGLIVVLCPPTFGQGIPAEDPVKVLVGRLDLEKY
                     KATIKGLTEFGDRREGTDRNRAAADWIERQLKSFGCNTARIKYEYNAPQRQWRPQSRA
                     PIPVASGEIRAGSGGSRIRGIAVPTGVNDDPEAQPDLKLRQINSQPSAPGAREQVYCT
                     KIGSTRPDEMYIVGAHMDGIGWGEAADDNASGTALVMEVARVLNSPDVRTERSIRFAL
                     WNNEETGLNGARAYVAQRAALQGIEDPPGSGRYPEPKWVGMVQHDMVLFDHGMPRADG
                     TLGPEQRPEADVNVEYQSTAKFADQAMKLAFFFRDANEKYATDYPAAVGHHMTNTDST
                     PFMDLVPAISVRENERGMQIGAGWNPHWHQPTDRYSTYSDKDFRLGLNAAQTTL"
     gene            1407..1817
                     /gene="rpsF"
                     /locus_tag="VEM33_06190"
     CDS             1407..1817
                     /gene="rpsF"
                     /locus_tag="VEM33_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYS51448.1"
                     /translation="MRHYELCFIVHPDQSEQVPAMIERYRTTVTSKNGKIHRLEDWGR
                     RQLAFPLSKVHKAHYVLMNIECDAETLGELEHSFKFNDAVLRHLIIRMERAWTTPSPM
                     MKEEKAKSVLGPGEAPRGEAPRSEAAKPAEPASA"
     gene            1821..2129
                     /gene="priB"
                     /locus_tag="VEM33_06195"
     CDS             1821..2129
                     /gene="priB"
                     /locus_tag="VEM33_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HYS51449.1"
                     /translation="MAQANRVTVAGRLIELDALRHTPAGVPMLKFRLQHDSVQTEAGG
                     ERKVSFEIAAVAFEREAKLLAAAKLGSSVKVNGFLAARSRSSRTPLLHATQIEFAEGD
                     "
     gene            2132..2440
                     /gene="rpsR"
                     /locus_tag="VEM33_06200"
     CDS             2132..2440
                     /gene="rpsR"
                     /locus_tag="VEM33_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HYS51450.1"
                     /translation="MPPPRGRGGFGKGKGKGKGRDGKREQKGSKQLFKRRKFCRFTAE
                     KIEWIDYKDVDLLKDFIGENGKIIPARITGTRTHYQRQLSAAVKRARYLALMPYTDLH
                     "
     gene            2451..2909
                     /gene="rplI"
                     /locus_tag="VEM33_06205"
     CDS             2451..2909
                     /gene="rplI"
                     /locus_tag="VEM33_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HYS51451.1"
                     /translation="MQVILLEKVVNLGGLGDVVKVKDGYARNYLLPQGKAKRATPESL
                     AEFEKKRAELEKAQSETLAAAQEKGAKLEGLMVQITQKAGVDGHLFGSVTNFDIVEAL
                     KGQGFEVERSMIRLPQGPLKTVGDHSIAVALHTDVVVHIKISVLGETPQQ"
     gene            3018..>3433
                     /locus_tag="VEM33_06210"
     CDS             3018..>3433
                     /locus_tag="VEM33_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HYS51452.1"
                     /translation="MAQPIPQTSSDSQLLNLKVPPHSIDAEQSVLGGLLIDNGAFDKI
                     GDVVTDGDFYRDDHRRIFRHVSRLIERGKPADVVTVDEAIKVSEDKDKTGGITYLAAL
                     AGNTPSAHNVRRYAEIVRECAVLRKLIEVSTEIADSA"
BASE COUNT          672 a         1067 c         1080 g          614 t
ORIGIN      
        1 cagcgtcgtc tgcgcagcgt tcagcccgag ccgaaaatcc ttgtccgagt acgtggagta
       61 gcggtccgtg ggctggtgcc aatggggatt ccatccggcg ccaatctgca tgccccgctc
      121 gttttcacga acactgatgg ccggaacgag atccatgaaa ggcgtggagt cggtattggt
      181 catgtggtgg ccgaccgcgg cgggatagtc cgtggcgtat ttctcgttgg cgtcccggaa
      241 aaagaacgcc agcttcatcg cctgatccgc gaacttcgcg gtggactggt attccacgtt
      301 gacgtccgct tcgggccgct gctccgggcc gagtgtgccg tcggcccggg gcatgccgtg
      361 gtcgaacagc accatgtcgt gctggaccat gccgacccac ttcggctccg gatagcggcc
      421 tgatcccggg ggatcctcaa tcccctggag tgccgctcgc tgcgcgacat aggcgcgggc
      481 gccgttaagg cccgtttcct cgttgttcca gagagcgaac cggatcgagc gctcggtccg
      541 cacgtcggga ctgttcagca cgcgcgccac ttccatgacc agcgccgtgc ctgaagcgtt
      601 gtcatcagcg gcctcgcccc agcctattcc gtccatatgg gcgccgacga tgtacatctc
      661 gtcgggccgc gtcgagccga tcttcgtgca atacacctgt tcgcgcgcgc cgggggcgct
      721 tggctgggag ttgatctggc gcagcttcag atcgggttgc gcttcaggat cgtcgttcac
      781 gcccgtgggc accgcaatgc cccggattcg cgagcctccg gagccggcgc ggatttcccc
      841 gctggccacg ggaatcggcg cgcggctctg cggtcgccat tggcgctgtg gcgcgttgta
      901 ctcgtacttg atgcgagcgg tattgcagcc gaagctcttc agctgacgtt cgatccagtc
      961 ggcggctgca cgattgcgat ccgttccctc tcgtcgatcg ccgaattcgg tcaggccctt
     1021 gatcgtggcc ttgtacttct ccagatcgag ccgcccgacc aggaccttca cagggtcctc
     1081 ggccggaatg ccctggccga acgtgggcgg gcagagcacc acgatcaacc cgccgaccgc
     1141 accggtaagt atcttggagc gcatgacctg tccttattaa aatggtcagc aaaacagccg
     1201 gatatcataa cttcgttcgc ctatcgcgat gcgatactac tgcagagcgc ggcgcttgct
     1261 ccgcagcccc tgattcggtt agaattcaac cacttcgacg accgctccgg cggtcgtttg
     1321 ttttagaccg ccaaaccctg ccgcaccagt ttccgccgcg tcgcaacggt ggaccgagcg
     1381 ggcggcatta tccataggga gcttgcatgc gacactacga actgtgtttc atcgtccatc
     1441 ctgaccagag cgagcaggtg ccggcgatga tcgagcgcta ccgcaccacg gtgacctcga
     1501 agaacggcaa gatccaccgg ctcgaggact ggggccgccg tcaactcgct tttccgctct
     1561 ccaaggtgca caaggcgcac tacgtgctca tgaatatcga gtgcgacgcc gagacactcg
     1621 gtgagctgga gcactcgttc aagttcaacg acgcggtgct gcggcacctg atcataagaa
     1681 tggagcgcgc gtggacaacg ccttcgccga tgatgaagga agaaaaagcg aagtcggtcc
     1741 tcggcccggg cgaagcaccg cgcggtgaag cgccgcgcag tgaggcggcc aagcccgcgg
     1801 agccggcgag cgcctgagca ttggcgcaag cgaatcgggt cacggtcgca ggccgcctga
     1861 tcgagctgga tgcgctgcgg catacgcccg cgggcgtccc gatgctgaaa ttcaggctgc
     1921 agcacgattc tgtgcaaacg gaagcgggcg gcgagcgcaa ggtgagtttt gagatcgctg
     1981 cggtggcatt cgagcgcgag gcgaaactcc tcgctgccgc gaagctgggg agcagcgtga
     2041 aggtaaacgg gtttcttgcc gcaaggagcc gatccagccg gacgcctttg ctgcacgcga
     2101 cacagatcga attcgcagaa ggagactaga catgccccca cccagaggtc gaggcggctt
     2161 cggaaaaggc aaaggaaaag gcaagggccg cgacggcaag cgcgaacaga agggctcgaa
     2221 gcagctcttc aagcgccgca agttctgccg cttcaccgcc gagaagatcg agtggatcga
     2281 ctacaaggac gtcgatcttc tgaaggactt catcggcgag aacggcaaga tcatcccggc
     2341 gcggatcacc ggaacgcgca ctcactacca gcgccagctc tcggcagccg tcaagcgcgc
     2401 gcgctacctt gcgctgatgc cgtacaccga cctgcactga aggaaccgcc atgcaagtca
     2461 ttcttctgga gaaagtcgtc aacctcggcg gcttgggcga cgtggtcaag gtgaaggacg
     2521 gttacgcccg caactatctt ctgccgcaag gcaaggcgaa gcgggcgacg cccgaaagcc
     2581 tcgccgagtt cgagaaaaaa cgcgctgagc tcgaaaaagc ccaatcggaa acgctggcgg
     2641 cggctcagga aaaaggcgca aagctcgaag gtctgatggt ccagatcacc cagaaggccg
     2701 gcgtcgacgg gcacttgttc ggctcggtca cgaatttcga catcgtcgag gcgctcaagg
     2761 ggcaagggtt cgaggtggag cgctcgatga tccgcctgcc gcagggcccg ttgaaaacgg
     2821 taggagatca ctccatcgcg gtcgccctgc acaccgacgt cgtcgtccac atcaagatat
     2881 cggtgctggg agaaaccccg cagcagtaac gcgggcaagc cgttgatacg gaagggccgc
     2941 gtctggcggc tctttttgtt tgtgcgtttc ctcgcgggac gcggtaaagt ctccgccaca
     3001 ggttttcagg cgctttcatg gcccagccca taccgcaaac cagctccgat tcccagctgc
     3061 tgaatctcaa ggttccgccg cattcgatcg acgctgagca gtcggtgctg gggggcctcc
     3121 tgatcgacaa cggcgccttc gacaagattg gcgacgtcgt caccgacggc gatttctacc
     3181 gggacgatca ccgccgcatc ttccgccacg tttcccggct gatcgagcgt ggcaagcccg
     3241 ccgacgtagt tacggtcgac gaggcgatca aggtaagcga ggacaaggac aagaccggcg
     3301 gcatcacgta tcttgcagcg cttgcaggca atacgccctc ggcgcacaac gtgaggcgct
     3361 atgccgagat cgtgcgcgag tgcgcagtcc tgcgcaagct gatcgaggtc tccactgaga
     3421 tcgccgacag cgc
//
LOCUS       DASPZD010000318         7527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_995845, whole genome shotgun sequence.
ACCESSION   DASPZD010000318 DASPZD010000000
VERSION     DASPZD010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995845"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VEM33_06215"
     CDS             complement(<1..528)
                     /locus_tag="VEM33_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51453.1"
                     /translation="MHTWRLSLAVLVAFAWPAVALPAAAEEPPAAAEPAGIEPEFVRP
                     LVPIARGRNDSNPVWSPAGDLVALERSRGDNKEIAIVRVNGDIVQTIHQQAQVAPGQS
                     AFFFPQVVEQTSYNSGISWSPNGNRIVFMSNGGEGNYDLYLREPDGRIARITTHREKD
                     EHAHWSPARDQIAFIS"
     gene            complement(549..3116)
                     /locus_tag="VEM33_06220"
     CDS             complement(549..3116)
                     /locus_tag="VEM33_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HYS51454.1"
                     /translation="MATTHVRFLPVLLATALLGACGERIDIEVKASIDGQPAPQAKVI
                     VDREELGVTDARGLFTQRLRKKAGTEVEVTVSKEAPGYRIEPWKTAFLVKLPKGDQIV
                     TYRVEADLKATRYVTVRVSEGGTPVPEAKVTIGGKEEGVTDAKGEFVYSYRTQPAKGA
                     EIGVAKTGFSAYRATRQLEPGQVVEVALNRQTVVAIKAMTDEYGRVSGVPGLSVSIDG
                     KTVGKTDAQGSYTYDYRGEPGKKAQIALAAPGYVPATWRTSVRLEGQVNLQHYFYPTA
                     PRPIRIGIYRVVGNTPGVDLKEVASQAEQALATQLFKFPAFREVPAERLQAEVNQRKL
                     NIERITTKGWQDTPLRASVDMIVLGSVAKDDGGYLVEVKFHTANGKIIFSEIARARNA
                     GGINGAVRDIAGNVIERFPLEGTVVGKEDDRYRINIGRNWRIARGTEFTLTTPTFAEG
                     GKVAGYRETGRIEVTRGDDSSSLVEVATLKQGEKVQIGDRVVRRIAREGEEGDERTYF
                     RLTAKGGIGTDVNPLAGANVYLNGEWAGATGTNGQAEVPLRLGRNYTLTLYRHGYQQL
                     TDKIRVGKSGEAREFLLAANNVLFKVDSTPSSATVYIDDEQIGKTPITGGKQITLGFH
                     SVRITYGEDYRDFFQVMEFTQKEEDRTGERRIVLQKDFLKIGERARQQGDIDGAIKAY
                     ASTEREHPDYAEAHRRLGDIYLDEKEDYDRAIAEFEAVLDLPENQQLIYKQFAVTFTN
                     LGHAYCEKGNRLVDGDRTAASTYFAKAIKALTTAKQNTRFFPSAEYDEAVHDTYYYTA
                     LSYHKLYLMTKQPAVMNSASLAWREYFDFFPKKLEGNPAFVQAREGARRYRDQIQEQ"
     gene            complement(3174..3650)
                     /locus_tag="VEM33_06225"
     CDS             complement(3174..3650)
                     /locus_tag="VEM33_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYS51455.1"
                     /translation="MNSLTINWRKIMTTRALVVLIAPLLVLSGCAATIHGTVQLIDSR
                     QQPVTNESAKGAVVNMINTTAPVDQASSSATVDEKGEFESTKGSIKPGIYKVEASRIG
                     YETRTETVEIGSFFGKKVEFKLPKIPEAKRKSIKGSASDEDKIINPGEVNIQPPAM"
     gene            complement(3945..5396)
                     /locus_tag="VEM33_06230"
     CDS             complement(3945..5396)
                     /locus_tag="VEM33_06230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYS51456.1"
                     /translation="MHKILIVEDVEAVADLQVLKLIRAGMTVETRRVETPQDFTRQLE
                     LYAPHAILCNVSVPRFSGVTALAMAQSLRPGTPFIFVFKTADEEAAVETFTRDAGDHV
                     FKTSPSNLPWTFGRAMKQAEERKTADGQGLAIARMTRMYADQVRELDQLQHQHLVFRK
                     LSRYLKAGVTPAEVYAAVECFGPQLFLRTNGRLYLVHSPGKHLESVASWGNRSPGEQT
                     FTVQDCWALRQTQAHWIGVPRTELLCGHVTSDSKLVPSYLCVPVLAQGETLGLLHLRR
                     VNEESTSPESGDSIESCLNLAMAVAEETGLALANLKVRDTLHEQSIRDPLTGLYNRRF
                     LEEFLLRELARADRKKHALSVITLDIDHFKRINDTLGHGAGDVVLRRVGLLLQGYVRE
                     SDIACRVGGEEFSLLLPEASMQIAAQRAENIRGGVHDLRLKYDGHNLGAITISLGVAA
                     FPEHGTTLDALIRAADQALYDAKSRGRDRVASA"
     gene            5516..7468
                     /locus_tag="VEM33_06235"
     CDS             5516..7468
                     /locus_tag="VEM33_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYS51457.1"
                     /translation="MTEQPDTFPRLLLQQARLRPGRPAFREKDLGIWQTWTWAQVADE
                     VRILACGLAALGFERGDRLAVIGDNRPRLYWAMMAAQALGGVPVPLYQDAVAAEMAFV
                     LQDADIGLAVVEDQEQVDKLLEVLPQCPRLKHICFDDPRGLRHYRQTELTGLEDLRAG
                     GREFERRNPGFFADELDKGRPSDVAVILYTSGTTGHPKGVSQTHAAMIQTARVLVAFD
                     GFVDKDEILCYLPMAWVGDFLYSYAQMHVAGFCLNCPESPDTVMTDLREIGPTYYFGP
                     PRVYENILTQVMIRIEDAGWIKRRIFHGFMAVAKRVGMGILEGKPGIPAKDRLLYRLG
                     EILVYGPLKNVLGMSRIRVAYTGGEAIGPDLFDFYRSIGINLKQLYGMTETCVTVCMH
                     ASGDVKLETVGPPLPGVEVRIADSGEVLVRSPGLMKGYHKRPDATREAIDAEGFFHTG
                     DAGYFDAGGHLKIIDRAKDVGRLADGTLFAPKYLENKLKFFPYIKEAVCFGQGRDKVC
                     AFINIDMAAIGNWAERRNLPYTGYADLASQPAVLDLIRECVEKANRDLAQDSTLANSQ
                     IRRFFVLHKELEADDGELTRTRKVRRRHIADKYAVLVDALYSDRTSCFVETQMRFEDG
                     RSGTVSADLRIVEAKTLDAAAARKAA"
BASE COUNT         1232 a         2412 c         2425 g         1458 t
ORIGIN      
        1 ggagatgaac gcgatctggt cgcgcgccgg cgaccagtgc gcgtgctcgt ccttctccct
       61 gtgagtcgtg atgcgcgcga tgcggccgtc gggctcgcgc aggtacaggt cgtagttgcc
      121 ctcgccgccg ttgctcatga acacgatccg attgccgttc ggcgaccagc tgattccgga
      181 gttatagctc gtctgctcca cgacctgggg gaagaagaac gcgctctggc ccggcgcgac
      241 ctgggcctgc tgatgaatgg tctggacgat gtcgccgttg acgcgcacga tcgcgatttc
      301 cttgttgtcg ccgcgcgagc gctcgagcgc gaccaggtct ccggcgggcg accacaccgg
      361 gttcgagtcg ttgcgccccc gcgcgatcgg aacgagcggc cgcacgaatt ccggttcgat
      421 cccggccggc tccgctgcag ccggcggttc ttcggcggcc gcaggcaagg caactgcggg
      481 ccacgcgaac gcgacgagca cggcaagcga aaggcgccag gtgtgcatcg cgttcgggcc
      541 gggcgcgatt actgttcctg gatctgatcg cggtatcggc gcgcgccttc gcgagcctga
      601 acgaaggccg gattgccttc gagctttttc gggaagaagt cgaagtactc tcgccacgcg
      661 aggctcgcgc tgttcataac cgccggctgc ttggtcatca gatacagctt gtggtacgag
      721 agcgcggtgt agtagtaagt gtcgtgcacc gcctcgtcgt actccgcgct cgggaagaaa
      781 cgcgtgttct gcttggcggt cgtgagcgcc ttgatcgcct tggcgaagta cgtggacgct
      841 gcggtgcgat cgccgtccac caggcggttg cctttctcgc aatacgcgtg gccgaggttg
      901 gtgaaggtga cggcgaactg cttgtagatc agctgctggt tctcgggcag gtccaggacc
      961 gcctcgaact cggcgatcgc tcggtcgtag tcctctttct cgtcgaggta gatatcgccc
     1021 agccgccggt gcgcctcggc gtagtccgga tgctcgcgct cggtggaggc ataggccttg
     1081 atggcgccgt cgatgtcgcc ttgctgccgc gcgcgctcgc cgatcttgag gaagtccttc
     1141 tgcagcacga tgcggcgctc gccggtgcgg tcctcctcct tctgggtaaa ctccatcacc
     1201 tggaagaagt cgcggtagtc ctcgccgtag gtgatgcgca cgctgtgaaa gccgagagtg
     1261 atctgcttgc ctccggtgat cggcgtcttg ccgatctgct cgtcgtcgat gtagacggtc
     1321 gccgacgacg gcgtagagtc gaccttgaac aggacgttat tggccgcgag caggaactcg
     1381 cgcgcttcgc cgctcttgcc gactctgatc ttgtcggtga gctgctggta gccgtggcgg
     1441 tacagcgtga gcgtatagtt gcggccgagc cgcagcggga cttccgcctg gccgttcgtc
     1501 ccggtcgcgc cggcccactc gccgttcagg tagacgttcg cgcccgcaag cgggttgacg
     1561 tcggtgccga tgccgccttt cgcggtgagc cggaagtagg tgcgttcgtc gccttcctcg
     1621 ccctcgcgcg cgatgcgccg caccacgcgg tcgccgatct gcaccttttc gccctgcttg
     1681 agcgttgcga cctcgacaag cgagctcgag tcgtcgccgc gcgtgacttc gatcctgccg
     1741 gtttcgcggt aacccgccac cttgccccct tcggcgaacg tcggcgtggt cagagtgaac
     1801 tcggtaccgc gggcgatacg ccagttcctg ccgatgttga tgcggtagcg atcgtcctcc
     1861 ttgccgacca ccgtaccctc gagcgggaag cgttcgatca cgttgcccgc gatgtcgcgc
     1921 acggcgccgt tgatcccgcc ggcgttgcgg gcgcgcgcga tctccgagaa aatgatcttg
     1981 ccgttcgcgg tgtggaattt gacctcgacc aagtagccgc cgtcgtcctt ggcgacgctc
     2041 ccgagcacga tcatgtccac gctcgcgcgc agcggagtgt cctgccagcc cttggtcgtg
     2101 atgcgctcga tgttgagctt gcgctggttg acctcggcct gcagcctctc cgcgggcacc
     2161 tcgcggaacg ccgggaactt gaacagctgc gtcgccagcg cctgctcggc ctgggacgcg
     2221 acctccttca ggtccacgcc cggcgtgttg ccgacgacgc ggtagatacc gatgcggatc
     2281 ggcctgggcg ccgtcgggta gaagtagtgc tggaggttga cttggccttc gagccggacg
     2341 ctcgttctcc acgttgcggg aacgtaaccg ggcgccgcga gcgcgatctg ggccttcttg
     2401 cccggctcgc cgcggtagtc gtacgtgtag ctgccctgcg cgtcggtctt gccgacggtc
     2461 ttgccgtcta tgctcacgct caagcccggc acaccgctaa cgcgaccgta ctcgtcggtc
     2521 atcgccttga tcgccaccac ggtctggcgg ttgagcgcga cttcgactac ctggcccggc
     2581 tcgagctgac gtgtcgcgcg gtaggcgctg aagccggtct tggcgacgcc gatctcggcg
     2641 ccctttgccg gttgcgtccg ataggagtac acgaattcgc ccttggcatc ggtgacacct
     2701 tcctccttgc cgccgatcgt caccttggcc tcgggcaccg gcgtaccccc ttcgcttacg
     2761 cgcacggtga cgtagcgcgt cgccttgaga tcggcctcga cgcggtacgt gacgatctga
     2821 tcgcccttag gaagcttcac gaggaacgcc gttttccaag gctcgatgcg gtagccgggc
     2881 gcctccttgc tcaccgtcac ttcgacctcg gtgccggcct tcttgcgcag gcgttgcgtg
     2941 aacaggccgc gggcgtcggt gacgccgagc tcctcgcgat ccacgatgac cttcgcctgc
     3001 ggcgcgggct ggccgtcgat gctcgccttg acctcgatgt cgatgcgctc gccgcaggcg
     3061 ccgagcaagg cggtggcgag caaaacggga aggaagcgca cgtgagttgt ggccatggtg
     3121 ataccctcgg ttatggcgtc gtcaggtcta agggacgcgg tgcatgcgtc cgctcacatc
     3181 gcgggcggct ggatattgac ctcgccggga ttgatgatct tgtcttcgtc gctggccgag
     3241 cctttgatcg acttgcgctt ggcctccggg atcttcggca acttgaactc gaccttcttg
     3301 ccgaagaagg agccgatctc gaccgtctcg gtccgcgtct cgtagccgat gcggctcgcc
     3361 tccaccttgt agatgccggg cttgatgctg cccttggtcg actcgaattc gcccttctcg
     3421 tccaccgtcg cggagctcga agcctgatcg accggcgcag tcgtgttgat catgttgacc
     3481 acggctcctt tcgcgctctc gttcgtgacc ggctgctgac ggctgtcgat gagctgcacc
     3541 gtgccgtgaa tcgtggcggc acagccggac aacaccagca gcggggcgat taggacaacg
     3601 agcgctcttg tggtcatgat ctttctccag ttgatcgtca gcgaattcac gggactgcag
     3661 ccagtcctcg ttctcgcgat tcggtgttcg ctttgatttt caaattgtgg atccgggttc
     3721 ggcacaatca agccgtggcg gcgcgaatgt ccttagaaca ttcgcgataa ccccacctga
     3781 cccgtggtct ggcgctcagc ggcgcggaca cacgacgctt tgtgtgtccg ccacatctcc
     3841 ccccctcgcc cagccgagct acaggcgcgt gtggagcggc gattataacc gagattcggg
     3901 aaagtctaag ggaggagggg tcggcacgtc cggatgagcg ccgctcatgc cgaggccacc
     3961 ctgtcgcggc cgcgggactt ggcgtcatac agcgcctggt ccgcagcgcg aatgagtgcg
     4021 tccagagtcg tgccatgctc cggaaaagct gcgacgccca gcgaaatggt gatagcgccc
     4081 agattgtggc cgtcgtactt cagcctcaag tcgtgcacgc ccccgcggat attctcggcg
     4141 cgctgtgccg cgatttgcat cgatgcctcc gggaggagca gcgagaattc ttccccgccg
     4201 acgcggcaag cgatatcgct ctcgcgaacg tacccctgca gcaggagacc cacgcgccgc
     4261 aggacgacgt cacccgcgcc gtggcccaag gtgtcgttga tgcgcttgaa gtgatcgata
     4321 tccagagtga ttacggaaag agcgtgcttc ttgcgatccg cgcgcgccag ctcccgcagg
     4381 aggaattcct ccaggaagcg gcggttgtac agaccggtga gcggatcacg gatcgattgt
     4441 tcatgcaggg tatcgcgaac tttcaagttg gcgagcgcaa ggccggtttc ttctgccacc
     4501 gccatcgcga gattcaaaca ggactctatc gaatcgccgc tttccggcga cgtcgattcc
     4561 tcgttcacgc gccgcaaatg cagcaatccg agcgtctcgc cctgtgccag gaccggcacg
     4621 caaaggtagg atgggaccag cttcgaatcc gacgtcacgt gtccacagag cagctcggtt
     4681 cgcggaaccc cgatccagtg agcctgcgtc tgacgcaagg cccagcaatc ctggactgtg
     4741 aacgtctgct cgcccggcga ccggttgccc cagcttgcga cgctctccaa atgctttccg
     4801 ggagaatgaa cgagatacaa cctgccgttc gttctcagga acagctgagg accgaaacat
     4861 tcgacggctg cgtaaacttc ggccggcgtc acgccggcct tcagatagcg gctcagtttg
     4921 cgaaagacga ggtgttgatg ctgcagttgg tccagttcgc gaacctggtc ggcgtacatg
     4981 cgcgtcatgc gcgcaatcgc gagaccctgg ccgtccgcag tcttgcgttc ttcggcctgc
     5041 ttcattgcgc gtccgaacgt ccacggaaga ttgctggggc tggtcttgaa aacgtggtcg
     5101 cccgcgtcgc gcgtgaacgt ttcgacagcg gcttcttcgt cggccgtttt gaaaacgaag
     5161 ataaacggcg taccgggacg caggctttgc gccatcgcca aagcggtgac tccgctgaac
     5221 cgcggcacgg agacgttgca gagtattgca tggggagcgt agagctccag ctgccgcgtg
     5281 aaatcctggg gcgtctcgac gcgccgcgtc tcaaccgtca tgcccgcgcg gatcagcttg
     5341 agaacttgta gatcggcgac cgcttcgaca tcctcgacga tcaggatctt atgcattggc
     5401 gatcgtcaca ggatgaagcg ctgcccccca acgagttacc gcgcgagctt ctcggctccc
     5461 tggacacgag agaactctga gagtgtaatt gaaaaccggt ctaaagtgag cacccgtgac
     5521 ggagcagcct gacacctttc cgcgccttct gctgcagcag gcgaggctgc ggcccggcag
     5581 gccggcgttt cgcgagaagg acctgggcat ctggcagacc tggacctggg ctcaggtcgc
     5641 ggacgaggtc cgtattctgg cctgcggcct cgccgccctg ggattcgagc gtggcgaccg
     5701 cctcgcggtg atcggcgaca accggccgcg cctgtactgg gccatgatgg ccgcgcaggc
     5761 cctgggcggc gtgcccgttc cgctctacca ggacgccgtg gccgccgaaa tggccttcgt
     5821 tctccaggac gccgacatcg gccttgccgt cgtcgaggac caggagcagg tcgacaagct
     5881 gctggaagtg ctgccgcagt gcccgcggtt gaagcacatc tgcttcgacg acccgcgcgg
     5941 gctgcgccat taccgacaga cggagctcac gggcctcgag gacctcagag cgggcggcag
     6001 ggagttcgag cggcgcaatc cgggcttctt cgccgacgag ctcgataaag gccggccctc
     6061 cgatgtcgcg gtgattctct atacgtcggg caccacgggt caccccaaag gcgtgtccca
     6121 gacgcacgct gcgatgatcc aaacggcacg ggtgctcgtg gccttcgacg gcttcgtcga
     6181 caaggacgag atcctgtgct atctgcccat ggcctgggtc ggcgattttc tctattccta
     6241 cgcgcagatg cacgtcgcgg ggttctgtct caactgcccc gagtcccccg acaccgtgat
     6301 gaccgatctg cgcgagatcg gcccgaccta ttacttcggg ccgccgcgcg tgtacgagaa
     6361 catcctgacc caggtgatga tccgcatcga ggacgcgggc tggatcaagc gccgcatatt
     6421 ccacggcttc atggctgtcg ccaagcgcgt ggggatggga atactcgaag gcaagcccgg
     6481 gataccggcg aaggaccggc tgctgtatcg gctcggcgag atcctcgtat acgggccgct
     6541 caagaacgtg ctcggcatgt cgcgcattcg cgttgcctac accggcggcg aggcgatcgg
     6601 tccggacctg ttcgatttct accgctccat cggcatcaac ctgaagcagc tttacggaat
     6661 gaccgaaacc tgcgtgacgg tgtgcatgca cgcctcgggc gacgtcaagc tcgaaaccgt
     6721 gggcccgccc ttgcccgggg tcgaggtacg tatcgccgat tcgggcgaag tgctggtgcg
     6781 ctccccgggt ctgatgaagg gataccacaa gaggcccgac gcgacgcgcg aggccatcga
     6841 cgcggagggc ttcttccaca ccggtgacgc gggttacttc gatgcggggg ggcacttgaa
     6901 gatcatcgac cgcgcgaaag acgtgggcag gctcgccgac ggcaccttgt tcgcgccgaa
     6961 gtatctcgag aacaagctca agttcttccc ctacatcaag gaggcagtgt gcttcggcca
     7021 ggggcgcgac aaggtctgcg cgttcatcaa catcgacatg gccgcgatcg gcaactgggc
     7081 cgagcggcgc aacctgccgt ataccggcta cgcggacctc gcctcgcagc ccgcggtgct
     7141 cgatctcatc cgcgaatgcg tggagaaggc gaaccgcgat ctcgcgcagg actccacgct
     7201 cgccaactcg cagatccggc gcttcttcgt cctgcacaag gagctcgagg ccgacgacgg
     7261 cgagctcaca cgcacgcgca aggtgcggcg gcgccacatc gcagacaagt acgcggtgct
     7321 ggtcgacgcc ctctactccg acaggacgag ctgtttcgtc gagacgcaga tgcgtttcga
     7381 ggacggccgc agcggaacgg tgtccgccga cctgcggatc gtggaggcga aaaccttgga
     7441 cgccgccgcg gccaggaagg cggcctgatt tccctttcgc ctgacatgac ggcaggcagg
     7501 aaaatcgggg ggccgatcct cgagatc
//
LOCUS       DASPZD010000319         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_990483, whole genome shotgun sequence.
ACCESSION   DASPZD010000319 DASPZD010000000
VERSION     DASPZD010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990483"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VEM33_06240"
     CDS             <1..1167
                     /locus_tag="VEM33_06240"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HYS51458.1"
                     /translation="LIEVENGRISAIEERIPAPPDAVALKGWTVPGFANVHSHAFQRL
                     LRGEIESGGGDFWEWRAQMYRFTEWDPADYFKHARLLFREMLEAGITAVGEFHYLHGH
                     GNELGEALIDAAREEGIRITLIDACYLRGGMDGGPLEAEQLAFSDGDADSWARRMDEL
                     KETNGARIAAAIHSVRAVDPESMRVVATYARERRMPLHIHLAEQAAEVEACLAVEEVT
                     PTELLQREGILGPDLTAVHAIHLNERDIRLLGENEVSICACSTTERDLGDRVGPLRAL
                     QAAGCDLAVGSDSNAVVDMLEEARGLELDQRRATGRRVLHQPEDLLRASTVNGMRTLG
                     WESGELRTGMLADFVTLDSPRDLWREMSPSYLIYGFSGRDVTNVVVGGETVVSR"
     gene            1164..>2070
                     /locus_tag="VEM33_06245"
     CDS             1164..>2070
                     /locus_tag="VEM33_06245"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzing the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HYS51459.1"
                     /translation="MRRLRNIGRLVTGSAAGVVERAAVICDGERIAWCGRLGDEPREL
                     MESVTEDHDCANGLVTAGLVDAHTHPVYAGDRTREVAMRSAGASYEEVAKAGGGINAT
                     VKATREEPISALEQATKRRLRTWLAGGATTVEAKTGYHLHREGELEATRMLARLSKRK
                     DLPRIEVTFLAAHALPPDRWARTGAYAKQVAEWCTDAYTAGARFCDVFCDRGYFSVPQ
                     SRAILKAGLKANLIPRIHADELDRTGGARLAAELHAVSADHLLRANRGDAIALARAGV
                     VATLAPGTALSIGKLPPVKDLISAGA"
BASE COUNT          360 a          684 c          721 g          305 t
ORIGIN      
        1 cttatcgagg tcgaaaacgg tcggataagc gcgatcgaag aacgcatccc tgcacccccc
       61 gatgccgtcg cactcaaggg ctggacggtg ccggggttcg ccaacgtgca cagccacgcg
      121 ttccaacggc tgctgcgcgg cgagatcgag tccggcggcg gcgacttctg ggagtggcgc
      181 gcccagatgt atcgcttcac tgaatgggat ccggccgact acttcaaaca cgcgcggctc
      241 ctgttccggg aaatgctgga ggcgggaatc accgcggtgg gagagttcca ctacctccac
      301 ggccatggca acgaacttgg cgaggcgctc atcgacgcgg cgcgtgagga gggcatccgc
      361 atcacgctca tcgacgcctg ctacctgcgc ggcgggatgg acggcgggcc gctcgaggcc
      421 gaacagctcg ccttcagcga tggcgacgcg gacagctggg cgcgccgcat ggatgagctc
      481 aaggaaacga acggcgcccg cattgcggcc gccattcaca gcgttcgcgc cgtggatccg
      541 gagtcgatgc gcgtcgtcgc gacctatgcc cgcgagcggc gcatgcccct gcacatccac
      601 ctcgccgagc aggccgcgga ggtggaggca tgcctcgcag tcgaagaggt cacgcccacc
      661 gagttactgc agcgcgaggg catcctcggt cccgacctga cagctgtgca cgcaattcat
      721 ctgaacgagc gcgacatcag gctgctcggc gaaaacgagg tctcgatctg cgcctgctcc
      781 acaacggaac gcgacctcgg cgatcgcgtc ggtccgctgc gcgcgctcca agccgcgggc
      841 tgcgacctcg cggtgggcag cgattccaac gcggtggtcg acatgctgga ggaggcgcgg
      901 gggctcgagc tcgaccagag gcgcgccacc gggcgcaggg ttctgcatca gcctgaggac
      961 ctgctgcggg cgtccacggt caacggcatg cgcacccttg gctgggaatc cggagagctc
     1021 aggacgggaa tgctcgcgga cttcgtcacg ctggattcgc ccagagacct gtggcgcgag
     1081 atgagcccgt cgtacctcat ctatggcttc tccggccgcg acgtgacgaa cgtcgtggtc
     1141 ggcggcgaaa cggtggtaag ccgatgagac gcctgcgtaa catcgggcgc ctggtcaccg
     1201 gctccgccgc gggcgtggtc gagcgcgccg cggtgatctg cgacggggag cggatcgcct
     1261 ggtgcggcag gctcggcgac gaaccacgcg agctgatgga gtcggtgacc gaggatcacg
     1321 attgcgcgaa cggcctcgtc accgccggtc tcgtcgacgc acacacgcac cctgtatatg
     1381 caggtgaccg aacgcgcgag gttgcgatgc gctcggccgg cgcgtcttac gaagaggtcg
     1441 ccaaggcggg cggcggaatc aacgccacgg tgaaggcgac tcgtgaagag ccgatcagcg
     1501 cgctcgagca agcgaccaaa cgcaggctgc ggacctggct cgccggcggt gcgacaaccg
     1561 tggaagcgaa gaccgggtat cacctgcacc gcgaaggcga gctcgaggcc acgcgcatgc
     1621 tcgcgcgcct gagcaagcgc aaagacctgc cgcgcatcga ggtaacgttc ctcgccgccc
     1681 acgcgctgcc gccggaccgg tgggcgcgca cgggcgcata tgcgaagcag gtcgcggagt
     1741 ggtgcaccga cgcgtacacg gcgggcgcgc gtttctgcga cgtgttctgc gaccgcggct
     1801 atttcagcgt gcctcaatcg cgcgccatcc tcaaggcggg cctcaaggcc aacttgatac
     1861 cgcgcataca cgccgacgag ctcgatcgaa ctggaggcgc gcgtcttgcg gcggagctgc
     1921 acgcggtttc agcggatcac ttgctccggg caaatcgcgg cgacgcgatc gcgctggccc
     1981 gcgccggggt ggtggccact ctggctcccg gcaccgcgtt gtcgattggc aagcttccgc
     2041 ccgtcaagga cttgatctca gccggcgccg
//
LOCUS       DASPZD010000320         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1034723, whole genome shotgun sequence.
ACCESSION   DASPZD010000320 DASPZD010000000
VERSION     DASPZD010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034723"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..546)
                     /locus_tag="VEM33_06250"
     CDS             complement(61..546)
                     /locus_tag="VEM33_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51460.1"
                     /translation="MLFPEMDPDDVPQPPQKPRGLRRFTRPVAYVACALAGAVLVTAI
                     YGLIGSRPGALGNRAGADSSPGSPVNDTAVLDRRVDTLALALSAFDMRARMFDTRRMG
                     CPALARGLQQVEDGWLAYNMARKDLMAASDPVRDARDKSLYADVRAVEVRFERSSCAR
                     P"
     gene            832..1680
                     /locus_tag="VEM33_06255"
     CDS             832..1680
                     /locus_tag="VEM33_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS51461.1"
                     /translation="MHLASISRRRGDDDAFPFTVPAIRSLDRLQLDAWVTFFVGENGS
                     GKSTLLEGIAAAARLATVGSAEVLRDDTLEAQRRLGKALRLSWRKRTTRGFFLRAEDF
                     FGFTKRMARLRAELAIEMMEAAQQYQASGRSDAALGLKQGPLRASIAELEKRYGAELD
                     ANSHGESFLKLFQSRFVPGGLYLLDEPEAPLSPQSQLALLAMIGDMVSKDAQFIIATH
                     SPMLLAYPDAQIYSFDTKPVRAVAYDELDHVRITRDFLNAPQRYLDQIFDKKTPSTQR
                     GEGARG"
     gene            complement(1702..>2099)
                     /locus_tag="VEM33_06260"
     CDS             complement(1702..>2099)
                     /locus_tag="VEM33_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51462.1"
                     /translation="VSTTSQIGTLGAGPQLTLGTGALRFYGFATMGGSLFWSNASYNS
                     CGCSASDWLDGHTTWMRSAGAGVLLGITAGHTPIAIDIAARGVRHDRVTYVPAGGLTQ
                     NSDGSFSARQVTTPVDMRVYQIGVSIGIR"
BASE COUNT          387 a          708 c          674 g          330 t
ORIGIN      
        1 tgcgcggcca gcggtgcgac gaacgacgcg aggacgaaaa ccccgaagcc ggatcgcacg
       61 ctacggccgg gcacaggacg aacgttcgaa ccgcacttcc accgcgcgca cgtccgcata
      121 caggctcttg tcgcgagcgt cgcgcacggg atccgaagcc gccatcaggt ccttgcgtgc
      181 catgttgtag gccagccatc catcttccac ctgctggaga ccacgcgcca acgccgggca
      241 ccccatccgg cgcgtatcga acatgcgggc ccgcatgtca aacgcagaca acgcgagcgc
      301 cagcgtgtcg acccggcggt ccaacacagc cgtgtcgttc accggcgagc ccggtgagga
      361 atcggcgccg gcccgattgc cgagcgcacc cgggcgagat ccgatgagcc cgtagatcgc
      421 ggtcacgagt acagcaccgg caagggcgca tgcgacatag gcgacggggc gggtgaaacg
      481 ccggagacca cgcggttttt gcggcggctg cggcacatca tccggatcca tttcggggaa
      541 gagcaccgca cggacctccc ggccggtcgc acttccgggc ggggacgacg ggaaccggac
      601 ggcgggctcg aaagcctgcg cggcgaccgg tcgtactccg aggctcggac tcgactccga
      661 catggcgtcc actgttgcga accggccgca agtcccgtac cctgcaaatc gccggtccgc
      721 tacgggatgg ttggctcctg gccggcttat ccctctggga cctgtaacac ttctgctaca
      781 ctcggtcgag gccactcatt ccagcccggt atctttgaac ctgtgagcgc gatgcacctg
      841 gcctccatca gtcgccgccg cggcgacgac gacgcgtttc cgttcaccgt ccccgccatt
      901 cggtcattgg atcggctcca gctggacgcc tgggtgacgt tcttcgtcgg cgagaacgga
      961 tccggaaaat cgaccctgct cgagggcatc gcggccgccg cgcggctcgc gacggttggc
     1021 agcgccgagg tcctccgcga cgacacgctc gaggcgcagc gtaggctcgg caaggcgctg
     1081 aggctcagct ggaggaaacg cacaacgcgc ggattctttc tgcgcgcgga agacttcttc
     1141 gggttcacca agcgcatggc gcgcctgcgg gccgagctcg cgatcgagat gatggaagcg
     1201 gcccagcagt accaagcgag cgggcggtcg gacgccgcgc ttggtttgaa gcagggaccg
     1261 ctgcgcgcct ccatcgcaga gttggagaaa cgctacggcg ccgagctcga cgccaattcg
     1321 cacggcgaaa gctttctcaa gctgtttcag tcgcgcttcg tcccgggcgg gctctacctg
     1381 ctggacgagc ccgaagcccc gctgtcgccg cagagccagc tcgcgctgct cgcgatgatc
     1441 ggcgatatgg tgtcaaagga cgcccaattc atcatcgcca ctcactcacc gatgttgctc
     1501 gcataccccg acgcgcagat ctacagcttc gacaccaagc cggtgcgggc ggtcgcgtac
     1561 gacgagctcg accatgtgcg catcacacgg gactttttga atgcgccgca gcgctatttg
     1621 gaccagatct tcgataagaa gaccccctct acgcaacgcg gagagggggc cagggggtga
     1681 agccggggac ggcaggggag gttaccgaat cccgatcgac accccgatct gatacacacg
     1741 catgtccacc ggtgtcgtga cttggcgtgc gctgaagctt ccgtcagaat tctgcgtcag
     1801 tccgccggcc ggcacgtacg tcacgcgatc gtgccgcact ccgcgagcgg caatgtcgat
     1861 cgcgatcggt gtgtgaccgg ccgtaatgcc gaggagcacg ccggcccccg cggaccgcat
     1921 ccaggtcgtg tgaccgtcga gccaatcgct ggcggagcag ccacagctgt tgtagctggc
     1981 attgctccag aacagggatc cgcccatcgt cgcaaacccg tagaaccgca atgcgcccgt
     2041 gcccaacgtc agttgcggcc cggcgccgag agtgccgatc tgtgaagtag tagaaacgc
//
LOCUS       DASPZD010000321         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_184353, whole genome shotgun sequence.
ACCESSION   DASPZD010000321 DASPZD010000000
VERSION     DASPZD010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184353"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VEM33_06265"
     CDS             <1..278
                     /locus_tag="VEM33_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HYS51463.1"
                     /translation="LDRDSGLVADAFDERDPAVKAMLRLAIQACRKAGKYVGICGQGP
                     SDHLDFAQWLVQEGIESLSLNPDTVVDTWLQLSREADTTAKAKVTAS"
     gene            complement(291..752)
                     /gene="smpB"
                     /locus_tag="VEM33_06270"
     CDS             complement(291..752)
                     /gene="smpB"
                     /locus_tag="VEM33_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HYS51464.1"
                     /translation="MRKPPRVTIVENRKASHEYFIEERYEAGIALEGWEVKAIRAGRA
                     QLKEAYVIVSNEEIFLIGAHISPLEAASTHVQADPVRTRRLLLHAEEIRRLIGKVDRA
                     GYTLVPIDLHYLRGRVKINIGLAKGKKQYDKRAAEKEKDWKREQQRLLRSR"
     gene            775..1212
                     /locus_tag="VEM33_06275"
     CDS             775..1212
                     /locus_tag="VEM33_06275"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HYS51465.1"
                     /translation="MTAVDRSALVAHSAQKMYALVADVESYPQFLPWCDRAVVSVGEP
                     GRTVATLHVSFRGMKKQFTTENFNRPDARIDMKLVSGPFRSLEGSWAFTALSDNACKV
                     QLSLRYQFASTLLEKAAGPAFHDITDSFVDAFVRRADEKFGSA"
     gene            1209..1499
                     /locus_tag="VEM33_06280"
     CDS             1209..1499
                     /locus_tag="VEM33_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HYS51466.1"
                     /translation="MSPSKISVEIVYALPDRQVLRRIMLPDGSTVEDAIQVSGLRAAF
                     PEMDTTHVGIHGEPVPLTTVLRDRDRVEIYRPLRADPKEVRRTRAAKKRAKG"
     gene            complement(1504..2007)
                     /locus_tag="VEM33_06285"
     CDS             complement(1504..2007)
                     /locus_tag="VEM33_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYS51467.1"
                     /translation="MPRIALLVVAFLVASSAQAQVHKCVDAAGKIIYTQDPCPANTKS
                     GTMSRNVLSAPAASPAASPADLPADKAAAAAKSTAPKTAAEQEQAFRQRQQEQAKASK
                     QAQQKSAEAQAKELNCRNARERLAQYDIGGRLSRITAQGERYYMDDAQIEQEKSRARA
                     DVAQACN"
     gene            2139..>3104
                     /locus_tag="VEM33_06290"
     CDS             2139..>3104
                     /locus_tag="VEM33_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HYS51468.1"
                     /translation="MRPVQKALTFDDVLLVPAHSSVLPRNVSLKTRLTRNIQLNIPLV
                     SAAMDTVTESRLAIAIAQEGGIGIVHKNLTAKKQAVEVAKVKRFESGVLRDPVTVSPD
                     ITVRELLALTQQHRVSGFPVVEKGKVVGMVTNRDYRFETNLDQPVRAIMTPRDRLVYV
                     KEGSSPEEARALLHKHRLERVLVVRDGFELRGLITVKDILKSSEHPDASKDQQGKLRV
                     GAAVGIGEGTEERVAALVEAGVDVIVVDTAHGHSQGVLDRLKWIKRNAGTAEVIGGNI
                     ATADGARALIDQGADGVKVGIGPGSICTTRIVAGVGIPQVTAIQSV"
BASE COUNT          549 a          978 c         1023 g          554 t
ORIGIN      
        1 gcctggatcg cgactcgggt ctcgtggccg acgcgttcga cgagcgcgac ccggcggtga
       61 aagccatgct gcgcctggcg atccaggcct gccgcaaggc cgggaaatac gtcggtatct
      121 gcgggcaggg gccctccgac cacctcgact tcgcccagtg gctcgttcag gaaggcatcg
      181 agagcctctc gctcaacccc gacactgtgg tggacacctg gctgcagcta tcacgcgagg
      241 ccgacacgac ggccaaagcc aaggtgacgg caagctagta ctacggccgg tcaccgcgaa
      301 cgcagcaagc gctgctgctc gcgcttccag tccttttctt tttcggcggc gcgcttgtcg
      361 tactgtttct tgccttttgc aaggccgatg ttgatcttga cgcgcccgcg caagtagtgc
      421 agatcgatgg gcaccagggt gtatccggcg cggtcgacct tgccgatgag ccggcggatc
      481 tcctcggcgt gcagcagaag cctgcgcgtg cgcaccggat cggcctggac gtgggtggat
      541 gcagcttcga gcgggctgat gtgcgcaccg ataaggaaga tctcctcgtt gctcacgatc
      601 acgtaggctt ccttcagttg cgcgcgtccc gcgcggatgg ccttgacctc ccacccttcc
      661 agcgcgatac ccgcctcgta gcgctcctcg atgaaatatt catgcgacgc cttgcggttt
      721 tcgacgatcg tcacgcgggg aggtttcctt atatgattgc tcgattctac aacgatgaca
      781 gccgtcgacc gatcggcgct ggtagcccac agcgcacaaa aaatgtacgc gctggtcgcg
      841 gacgtggaat cctacccgca gttcctgccg tggtgcgacc gggcggtcgt ttccgtgggc
      901 gaaccgggcc ggaccgtcgc cacattgcac gtcagcttcc gcgggatgaa aaagcaattc
      961 acgactgaaa atttcaaccg gcccgatgca cgcatcgaca tgaaactggt gtcgggcccc
     1021 tttcgcagcc tggagggaag ctgggcgttc accgcgcttt ccgacaacgc ctgcaaagtg
     1081 caattgagcc tgcggtacca atttgccagc acgctgctcg aaaaggccgc cggcccggcg
     1141 tttcatgaca tcacggacag cttcgtcgac gcgttcgtgc gccgcgcgga cgagaaattc
     1201 ggcagcgcgt gagcccgtcc aagatctccg tcgaaatcgt ctacgcgctt cctgaccgcc
     1261 aagtgctgcg ccggatcatg cttccggacg gaagcacggt cgaggacgcg attcaggtga
     1321 gcggcctgcg cgcggcgttt ccggaaatgg acacgacgca cgtcggaatc catggagaac
     1381 cggttcccct caccacggtc ctgcgcgacc gggaccgcgt tgagatctac cggccgctgc
     1441 gggccgatcc gaaggaagtc aggcgaacgc gcgccgcgaa gaagcgcgcg aagggatgag
     1501 gctttagttg caggcctgcg ctacgtcggc gcgcgcgcgg cttttctcct gttcgatctg
     1561 cgcgtcgtcc atgtagtagc gctcgccctg cgcggtgatg cgggaaaggc gcccgccgat
     1621 atcgtattgc gcgaggcgct cccgggcgtt gcggcagttc agttccttgg cctgggcctc
     1681 cgcggatttc tgctgggctt gcttcgaggc ctttgcctgc tcctgctggc gctggcggaa
     1741 cgcctgttcc tgctccgccg cggtcttggg cgcagtgctc ttggccgcgg ccgccgcctt
     1801 gtcggcgggc aaatcggcgg gggaggcggc gggagaagca gcgggagccg agagaacgtt
     1861 gcgggacatc gtccccgatt tcgtattcgc ggggcagggg tcctgcgtat agatgatctt
     1921 gcccgcggca tcgacgcact tgtggacctg cgcctgcgcg ctcgatgcga cgagaaatgc
     1981 gacgacaagc agcgcgattc gaggcatgac gaattcccct tgacgggtga ggcgcaccat
     2041 agcgagtttt cgcgcacgcg tcaatcgagg gtgcaggcga aacccaagaa tttaccggga
     2101 gttgcgaccg gcgtataatc cgacttttgg gtgtggcaat gcgtccagtc cagaaggctc
     2161 tcacctttga cgacgtcctt ctcgtaccgg cccactccag cgtcctgccc cgaaatgtca
     2221 gcctcaagac ccggctgacg cgcaacatcc agctcaacat cccgctcgtt tcggcggcga
     2281 tggacaccgt caccgagtcg cgcctggcga tcgccatcgc gcaggaaggc ggcatcggca
     2341 tcgtccacaa gaacctcacc gccaaaaagc aggccgtgga agtcgccaag gtcaagcgct
     2401 tcgaatcggg cgtgcttcgc gatccggtga cggtgtcgcc ggacatcacg gtgcgcgagc
     2461 ttctcgcgct cacgcagcaa caccgggtat cgggattccc ggtggtggag aagggcaagg
     2521 tcgtgggcat ggtcaccaac cgcgactacc gcttcgagac caacctcgac cagccggtgc
     2581 gcgccatcat gaccccgcgc gatcggctgg tgtacgtcaa ggagggatcg agcccggaag
     2641 aagcgcgcgc gctcctgcac aagcatcgcc tcgagcgcgt gctggtcgtg cgcgacggct
     2701 tcgagctgcg cggcctgatc acggtgaagg acatacttaa gtcgagcgag cacccggatg
     2761 cttcgaagga ccagcagggc aagctgcggg tcggtgcggc ggtcggtatc ggggaaggca
     2821 cggaggagcg cgtcgccgcg ctggtcgaag ccggcgtgga cgtcatcgtg gtggacacgg
     2881 cgcacggtca ttcgcagggc gtgctcgacc gcttgaagtg gatcaagcgc aatgccggca
     2941 cggcggaagt gatcggcggc aatatcgcca ccgcggacgg cgctcgagcg ctcatcgatc
     3001 agggggcgga cggcgtgaaa gtcggcatcg gaccgggctc gatctgcacc acgcgcatcg
     3061 ttgccggggt cggaatcccg caggtcacgg cgatacagag cgtc
//
LOCUS       DASPZD010000322         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1531986, whole genome shotgun sequence.
ACCESSION   DASPZD010000322 DASPZD010000000
VERSION     DASPZD010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531986"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VEM33_06295"
     CDS             complement(<1..192)
                     /locus_tag="VEM33_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51469.1"
                     /translation="MMRVRVMKLKKTSLAAAAALALGSASAVHAEIGLKAGAWDLSFS
                     GNVNGFATWNSCDTSATVVA"
     gene            372..1481
                     /locus_tag="VEM33_06300"
     CDS             372..1481
                     /locus_tag="VEM33_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HYS51470.1"
                     /translation="MDVRAAVAHKAGAPLSIETVQLQGPKAGEVLVEIKATGICHTDE
                     FTRSGSDPEGLFPAILGHEGAGVVVEAGPGVASVKKGDHVIPLYTPECRQCEYCLSGK
                     TNLCQAIRVTQGQGVMPDGSSRFSLGGKKLFHYMGTSTFANYTVVPEIALAKIREDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVVQGARMAGANMIVGVD
                     TNPARKALAEKFGITHFVNPKEAGSELVAHLVNLTKGGADYSFECIGNVDVMRQALEC
                     CHKGWGVSVIIGVAGAGQEIKTRPFQLVTGRVWKGTAFGGAKGRRDVPKIVDWYMERK
                     INIDDLITHKLKLADINQGFDLMHEGKSIRSVVLY"
     gene            1478..2314
                     /gene="fghA"
                     /locus_tag="VEM33_06305"
     CDS             1478..2314
                     /gene="fghA"
                     /locus_tag="VEM33_06305"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HYS51471.1"
                     /translation="MITTVSRSKCFGGMQAVYSHEARETACVMRFGVFLPPQAEARKR
                     PVLYWLSGLTCTEENFIVKAGAQRVAAELGLAIVVPDTSPRGLKIPGESDNYDFGLGA
                     GFYVDATQAPWSRGYRMYSYVVKELPEVIESAFPVDPARTGIFGHSMGGHGALTIALK
                     NPQTYKSVSAFAPICSTMRCPWGEKALAGYLGADRALWREYDATALVETRGWKGPALL
                     VDQGTNDQFLESQLKPELLKEACRRSGVPLDLRLQAGYDHSYFFIASFIEDHLRFHAR
                     NL"
     gene            complement(2337..3047)
                     /locus_tag="VEM33_06310"
     CDS             complement(2337..3047)
                     /locus_tag="VEM33_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYS51472.1"
                     /translation="MRSKALRLAVTVALFSALLAGCAPLLRGPAPAPAAPPADFPEGY
                     YREALAHGKRVFRVDSRESLVVLEVRRSGSLARLGHDHVVASREVVGYVAPDEGRADL
                     YVALARLVVDEPALRAEAGFDTQPSESDIEGTRANMLEKVLEAEKFPFALIRVSGASA
                     KRGEVTLNVAITLHGSTRSLQVPAEIDADAEKMSVTGRFSFDQTDFGITPYSLLGGAI
                     AVKNRVELRFRIMSMSSL"
     gene            complement(3049..>3272)
                     /locus_tag="VEM33_06315"
     CDS             complement(3049..>3272)
                     /locus_tag="VEM33_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYS51473.1"
                     /translation="NRYVARGILLLKGVKQPVEVPFTWTASADGAVMEGELTVKRGAF
                     GIGTGEWAATNVIGADVKVKFKVKLRKAG"
BASE COUNT          616 a         1068 c         1029 g          559 t
ORIGIN      
        1 cgcgacgaca gtggccgaag tatcgcagct gttccaggtt gcaaagccgt tgacgttgcc
       61 cgagaacgaa aggtcccacg cgccggcctt caaaccgatc tccgcgtgca ccgcagaagc
      121 gctccccaga gcgagagcgg ccgccgctgc aagcgaagtc ttcttgagct tcataactct
      181 gactcgcatc atctgtacct cctccgttta gaatccaacc aagcaatgcc tggtttgcac
      241 aggacagcat gaacctcccg cggataagtt actcgcattg cggcccgaat gtatactcgt
      301 tgcacatcct gcggcaagcg ttccataatt gacgcaccgt tccttcacgt tcaattcaag
      361 gggagcaaac catggatgtt cgcgcggcag tcgcccataa agcgggggcg cctctaagca
      421 tcgagaccgt gcagttgcag gggccgaagg ccggcgaagt gctggtcgag atcaaggcga
      481 ccggcatttg ccacaccgac gaattcacgc gctcggggtc ggaccccgag ggtttgttcc
      541 cggcgattct gggccacgag ggcgcgggcg tggtggtcga ggcgggaccg ggagtcgcga
      601 gcgtgaaaaa aggcgatcac gtcattccgc tctacactcc cgaatgccgg cagtgcgaat
      661 attgcctctc cggcaagacc aacctgtgcc aggcgatacg cgtcacgcag gggcaaggtg
      721 tgatgccaga cggcagcagc cgcttctcgc tcggcggcaa gaagctcttc cactacatgg
      781 gcacttcgac tttcgccaac tacaccgtgg tgcccgaaat cgcgctggcg aagatccgcg
      841 aggacgcgcc gttcgacaag gtgtgctaca tcggctgcgg cgtgaccacc ggcatcggcg
      901 cggtgatcaa caccgccaag gtcgagccgg gcgcgaacgt cgtggtattc ggcctgggcg
      961 gcatcgggct gaacgtcgtg cagggcgcgc gcatggccgg cgccaacatg atcgtcggcg
     1021 tggataccaa tccggcgcgc aaggcgctcg ccgagaaatt cggcatcacg catttcgtca
     1081 acccgaagga agccggcagc gagctggtcg cgcacctggt gaacctgacc aagggcggcg
     1141 ccgactacag cttcgagtgc atcggcaacg tcgacgtgat gcgccaggca ctcgagtgct
     1201 gccacaaggg ctggggcgtg tcggtgatca tcggcgttgc gggcgcgggc caggagatca
     1261 agacccgccc cttccagctc gtcaccggcc gcgtatggaa gggcaccgcg ttcggcggcg
     1321 ccaagggccg gcgcgacgtg cccaagatag tcgactggta catggaacgt aagatcaaca
     1381 tcgacgacct gatcacgcac aagttgaagc tcgccgacat caaccagggc ttcgacctga
     1441 tgcacgaggg caaatcgatc cgctcggtgg tgctctactg atcaccaccg tctcccgcag
     1501 taaatgtttc ggcggcatgc aggccgtcta cagccacgag gcgcgcgaaa ccgcgtgcgt
     1561 catgcgcttc ggcgtttttc ttccgccgca ggcggaagcg cgcaagaggc cggtgctcta
     1621 ctggctctcg ggtctcacct gcacggaaga gaatttcatc gtcaaggcgg gcgcgcagcg
     1681 cgtcgccgcg gagctgggac tcgccatcgt cgtcccggat accagcccgc gcggcctgaa
     1741 gattccgggc gaaagcgaca actatgattt cggcctgggc gccggattct acgtggacgc
     1801 cacgcaggcg ccctggtcac gcggctaccg gatgtattcc tatgtcgtga aggaattacc
     1861 cgaggtgatc gaatccgcgt ttcccgtcga ccccgcgcgc actggcatct tcggccattc
     1921 catgggcggg cacggcgcgc tgaccatcgc gctcaagaat ccgcaaacat acaagtcggt
     1981 gtcggctttt gccccgattt gctcgactat gcgctgcccc tggggagaga aagcgctggc
     2041 cggatatctc ggagccgatc gcgccctctg gcgggaatac gacgcgaccg ccctcgtcga
     2101 gacccgcgga tggaaggggc cggcgctgtt ggtcgatcag ggcacgaacg accagttcct
     2161 ggagagccag ctcaagcccg agctgctgaa agaagcctgc aggcgaagtg gtgtccccct
     2221 ggatcttcgc ctgcaagcgg gttacgacca cagctacttt ttcatcgcca gcttcatcga
     2281 ggatcacctt cgattccatg cgcgcaatct gtaacgcagg agcgcctgat gagacttcac
     2341 agtgaggaca tcgacatgat gcggaaacgc agctcgacgc ggttcttcac cgcgatggcg
     2401 ccgccgagga gcgagtaggg cgtgatgccg aaatcggtct ggtcgaacga gaaccgcccg
     2461 gtcacgctca ttttttccgc gtcggcgtcg atctccgccg gcacctgcag cgagcgggtg
     2521 ctcccgtgca gagtgatcgc gacgttcagc gtgacctccc ctcgtttcgc gctcgctccg
     2581 ctcactcgga tgagcgcgaa cggaaacttc tccgcttcca gaaccttttc cagcatgttg
     2641 gcgcgcgttc cctcgatgtc ggactcgctc ggctgggtgt cgaaaccggc ctctgcgcgc
     2701 agcgccggct cgtccaccac caggcgcgcg agcgcgacgt acaggtcggc gcggccttcg
     2761 tccggcgcga cgtagccgac tacttcgcgg ctggcgacga cgtggtcgtg ccccaggcgc
     2821 gcgagcgaac ctgagcggcg cacttcgagc acgaccagcg actcgcgcga atcgacgcgg
     2881 aacacgcgct tcccgtgtgc cagagcctcc cggtaatagc cttcggggaa atccgccggc
     2941 ggagctgcgg gcgcgggcgc cggcccgcgc aggagcggcg cgcacccggc gagcagcgct
     3001 gagaagagcg ccaccgtgac agcaaggcga agagccttcg agcgcatctc agccggcctt
     3061 gcgcagcttc accttgaact tcactttcac atcggcgccg atgacattgg tcgccgccca
     3121 ctcgccggtg ccgatgccga atgccccgcg cttcaccgtc agctcgcctt ccatgacagc
     3181 gccatcggcg gaagccgtcc aggtgaacgg cacctcgacc ggctgcttca cgcctttcag
     3241 cagcaagatg ccgcgcgcca cgtagcgatt gc
//
LOCUS       DASPZD010000323         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_965927, whole genome shotgun sequence.
ACCESSION   DASPZD010000323 DASPZD010000000
VERSION     DASPZD010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965927"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VEM33_06320"
     CDS             <1..268
                     /locus_tag="VEM33_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51474.1"
                     /translation="AGTLSPSGRFVAGSYQSASAPTSNDIESLTTTGGGTVWPNAATT
                     LWAGATTVQQGERVFVVTREGNRINTGASGAGVVSILESHEVVP"
     gene            555..1292
                     /locus_tag="VEM33_06325"
     CDS             555..1292
                     /locus_tag="VEM33_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HYS51475.1"
                     /translation="MCTTLLEPADYQILLVESPKVPTDELKAAIRWRIKDLLEYHIDD
                     ATVDVFEVRAEGDAKGSAKAMYAVATPNEVVQKRIALFQGARVPLKVIDIPEMAQRNI
                     AALFESPDKATAMLAFSPWGGMLTISFRGELLLTRRLEVTSVQLGQREHGDHYRERVA
                     TEITRSLDIFDRQRLSVGVGELLLAPLPQDPELEQFLRGKLYVPVVPAALPQVMDFAD
                     AGEPTAEEQWEYFYLFGAALRVEEKAL"
     gene            1289..1936
                     /locus_tag="VEM33_06330"
     CDS             1289..1936
                     /locus_tag="VEM33_06330"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HYS51476.1"
                     /translation="MSQQINLFNPLFLEKKKYFSAVTMTQAIGLIVLGMAVFYVFAFW
                     QDRNLARQIGESGRAYEQQKQQFAKIAAELSPEKREAQLDQDLKSIEAAIALRRSLMG
                     ELGPGGRAGPAGYSEYLRAFARQTVQGLWLTSIQIAEGGGQLTMSGRALQADLVPVLI
                     GRLKQEPVLRGRPLEGLAITRSIWGKDNPRAIVDFTVTSQGFPEQRSPLAERAKP"
     gene            1939..2604
                     /gene="gspM"
                     /locus_tag="VEM33_06335"
     CDS             1939..2604
                     /gene="gspM"
                     /locus_tag="VEM33_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HYS51477.1"
                     /translation="MRFPVPLQRYADRLDAMSLRERVLIFLAVAVVIVAIADSALFDP
                     ISRRQKVNSQRIHQQEDEIRTMQGQVQAYAQGRTGDNAGAKRERLEKRKVELAALDRE
                     LAAKRNDLVPPERMAKMLYEIVKRTPDVELVSLRSLPATGLAQSLTAGLSTSGLALYR
                     HGIEITVSGSYLRMLTYVNQLERLPAKIMWGNMELQAAAYPVTTLKITLYTLSPEKTW
                     LLI"
BASE COUNT          527 a          804 c          858 g          480 t
ORIGIN      
        1 ggcgggcacg ctatcgcctt caggccggtt tgtcgcaggc tcctatcagt ccgcttcggc
       61 accgacgagc aatgatatcg aatcgctgac gacgaccggg ggcggaacgg tgtggccgaa
      121 cgccgcaacg acgctctggg ctggcgcgac gaccgtgcaa cagggtgagc gggtcttcgt
      181 cgtgacgcgg gaaggaaacc gaatcaacac cggcgcaagc ggtgcgggtg tggtgtctat
      241 tcttgagagt cacgaagttg tgccctagca ggactcgcca caagaaaagt cattgacaac
      301 aataccttat tcatataact tgaagttaag catgattcag ggagagggta ttgctgggtt
      361 ggttcagcaa atcggcgcag cgttcaggac ttctggccgt cgcaatcgag ccggaagcga
      421 tcaaattcgc ccatgtgcgc gccgcgaacc gcgaacggcc tcttgcgacg cgctggtgga
      481 gtgttccatg cccgccatcc gagcaggcgg attcgctgca gagggagacg aaggagcacg
      541 gactcgcccg tttcatgtgt actacgctcc tcgagcccgc ggactaccag attctgttgg
      601 tggaatcgcc gaaggttccc acggacgagc tcaaggccgc gattcgctgg cggatcaagg
      661 atctgctcga atatcacatc gacgacgcca cggtcgacgt attcgaagtg cgcgccgagg
      721 gcgacgccaa gggatcggca aaggccatgt acgcagtcgc cactcccaac gaggtcgtac
      781 agaagcgcat cgccctgttt cagggtgccc gcgttcccct caaagtcatc gatatccccg
      841 agatggcgca acgaaatatc gcggcgttgt tcgagtcgcc cgacaaggcg accgcgatgc
      901 tcgcgttttc cccttggggc ggaatgttga cgatctcgtt tcgcggcgag ctgcttctca
      961 cgcggcgcct cgaagtcacg tcggtgcagc tgggacaaag ggagcacgga gaccattatc
     1021 gcgagcgggt tgcgaccgag atcacccgct cgctcgacat ctttgatcgc cagcgcctga
     1081 gcgtcggggt gggagagctg ctgctcgccc cgctgccgca ggaccccgag ctcgagcagt
     1141 ttctgcgcgg caagctctac gtgccggtcg tgcccgccgc attgccgcaa gtcatggatt
     1201 ttgcggacgc cggcgagccc actgccgagg agcaatggga gtatttttac ctgttcggcg
     1261 cggccttgcg ggtcgaggag aaggcgctgt gagccagcag atcaatcttt tcaatccgct
     1321 tttcctcgag aagaagaagt atttttccgc ggtgaccatg acccaggcga tcggactgat
     1381 cgtgctgggt atggcggttt tctatgtctt cgccttctgg caggacagga acctcgcgcg
     1441 gcagatcggg gaatcggggc gcgcctacga acagcagaag cagcagttcg caaaaatcgc
     1501 tgcggagctc agcccggaga aacgcgaagc gcagctcgat caggacttga agagcattga
     1561 agccgcgatc gccttgcggc ggtcgctcat gggcgagctc ggaccaggcg gtcgggctgg
     1621 tcccgccgga tactcggaat acctccgcgc ctttgcgcgg cagaccgtgc aaggcctctg
     1681 gctcacgagc atccagatcg cggaaggtgg cggacagctg accatgagcg gccgggcgct
     1741 gcaggcggat ctcgttcccg tgctcatcgg gcgactcaag caggagcccg tgcttcgggg
     1801 gcgcccgctc gagggcctgg ccattacccg tagcatctgg ggaaaggaca atccccgcgc
     1861 gatcgtggat ttcaccgtga cctcgcaggg atttcccgag cagcgaagtc ccctcgcgga
     1921 gagagcgaag ccctaaccat gcgcttcccg gtgccgttgc agcgctacgc cgatcgcctc
     1981 gatgcgatga gcctgcgcga gcgcgtgctc atatttctcg cggtggccgt cgtgatcgtc
     2041 gcgatcgccg acagcgcgct cttcgacccc atctcgcgga ggcagaaggt caattcgcag
     2101 agaattcacc agcaggagga cgaaattcgc accatgcagg ggcaggtgca ggcgtatgcc
     2161 caggggcgaa cgggtgacaa cgcgggcgcg aagcgggagc ggctggaaaa gcgcaaagtc
     2221 gaactcgccg cgctcgatcg cgaactcgcc gcaaaacgaa acgacctggt tccacccgag
     2281 cgcatggcga agatgctcta cgaaatcgtg aagcgcactc ccgacgtcga gctcgtcagc
     2341 ctgcgctcgc tgcctgcgac cgggttggcc cagtcgctca cggcggggtt gagcacgagc
     2401 ggcctcgcgt tgtatcggca cggaatcgag atcacggtgt ccgggagcta tctgagaatg
     2461 ctcacctacg tgaatcagct cgagcgcctt cccgcgaaga tcatgtgggg caacatggag
     2521 ctccaggccg ctgcctaccc tgtgacaacg ctgaagatca ccctctatac gctgagcccg
     2581 gaaaaaacat ggctgcttat ctgaccctgg gtccagcgct cgtcatgctg atcggcgcgc
     2641 cgtgcgtctt cgcacaaggg ctgagcgat
//
LOCUS       DASPZD010000324         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1009211, whole genome shotgun sequence.
ACCESSION   DASPZD010000324 DASPZD010000000
VERSION     DASPZD010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009211"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..805)
                     /locus_tag="VEM33_06340"
     CDS             complement(107..805)
                     /locus_tag="VEM33_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HYS51478.1"
                     /translation="MSSVRRTALVAGILYLITFVSSIPAVILLDPVLSNPNYIISAGA
                     DTRVIGGALLDLVNALACIGTAVALFSIVKRQHEGFALGFVTSRMFEAAVIVIGVVSI
                     LSVVSLRQGDASGADAASLVTAGRSLVAVRNWTFALGPGLAPAINALLLGTLMYRSRL
                     VPRIIPMLGLIGAPMLLSSTLGTMFGINSAVSIWTGIATLPIFFWELSLGLWMTFKGF
                     NASAPILSRASEGS"
     gene            complement(967..1395)
                     /locus_tag="VEM33_06345"
     CDS             complement(967..1395)
                     /locus_tag="VEM33_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51479.1"
                     /translation="MGDDLAASNVNRSYSLAATSIAIFTFMLIFMYPRFASGEVNPLL
                     FQATLVVMGVATFSFVFASFYYYASSLGGRIDDAERALYARRGDRFWVLGYTLLFVDP
                     SLILFSVGLFAVGSAWFALWLVYVLFVIRYFPRVQTARKS"
     gene            1596..2090
                     /locus_tag="VEM33_06350"
     CDS             1596..2090
                     /locus_tag="VEM33_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HYS51480.1"
                     /translation="MSIVVPPRGTRGSEFPGFARALMRVMQGPFQMAFRRFGDRMRVQ
                     GRPLVELETVGAKSGSRRHAVVGSFPEIAAAVSSSWVVVASNSGSARHPAWFLNLAKK
                     PDQVWITTGKRRVKVRPETLEGVERERAWREITSLAPGYGRYVELTDRVIPIVRLRQE
                     TKEA"
BASE COUNT          479 a          645 c          731 g          368 t
ORIGIN      
        1 acttgggacg cccgtaggaa ctatgggcgc tcgatgagca cgaccacgcg ggccacgcct
       61 acgccgtccg tgatggcggt ttaggccgct cccggaccaa cttcggtcac gacccctctg
      121 atgcccggct gaggatcgga gcagatgcgt tgaagccctt gaaggtcatc cagaggccga
      181 gggacagctc ccagaagaag attgggagag tcgcaatccc tgtccaaatc gacacagccg
      241 agttgatccc gaacatcgtg cccagcgtcg acgacaggag catgggtgca ccgatgagtc
      301 cgagcatcgg aatgatgcgc ggaaccagtc gcgaccggta catcagggtt ccgagcagca
      361 gggcattgat agccggagcc agccctggtc cgagagcgaa cgtccagttg cggaccgcca
      421 caagtgaccg acccgctgtg acgaggctag ccgcatcggc gccggaggcg tcgccttgcc
      481 gcaggctcac gaccgagagg atgctgacga cgccgattac gatgacggct gcctcgaaca
      541 tacggctggt gacgaagccg agggcgaatc cctcgtgctg gcgcttgacg atcgaaaaca
      601 gcgcaactgc agtgccgatg cacgcgagcg cgttgacaag atcaaggagc gcgccgccga
      661 tcacgcgcgt gtcggcgccg gcgctgatga tgtagttggg gttggacaag accgggtcca
      721 gcaggatgac cgccgggatc gagctcacga aagtgatcag gtagaggatt cccgcaacga
      781 gtgcggttct ccgaactgag ctcacgacga tgctccttca aggtgggtcg cggcgggaca
      841 cagcgtcgtt ctgagtgagg catcgagcga atcaagcgtc attttggctc ggtcatgcta
      901 ggccgcgttt catcgcagga gttgtccgcg agctgcgggt gacgattcct gcgcgggctc
      961 agcgacctaa ctcttccgcg cggtctggac gcgggggaag tagcggatca cgaaaagcac
     1021 gtagaccagc cagagtgcga accatgcaga cccgacggca aagagcccga ctgagaagag
     1081 gatcaagctc ggatccacga acagcagggt gtatccaagc acccagaagc gatctccacg
     1141 acgggcgtac agagcccgct cggcatcgtc gatccggccg ccaagcgatg aggcgtagta
     1201 gtagaaggac gcgaacacga atgagaacgt cgcgacgccc atcacgacca aggtggcttg
     1261 aaacagcaag gggttgacct cgccactcgc gaacctcgga tacatgaaga tcagcatgaa
     1321 cgtgaagatc gcgatgctcg tcgctgcgag cgaatacgag cgattgacgt tagatgcggc
     1381 aagatcgtct cccatggaaa tgcctcctca gttcgtcacc ccgtgcaggg actaacaaac
     1441 atttcacatc cagcaggtga aagaaactgc gacgtgtttt ggcacaaccc cgcgaaggaa
     1501 taggaaactt agaggagcga cgacgcgtgc gggtttgcgc gccaaccgga ggtcttcgcg
     1561 cggcgccgtg gccgcggcta cgcgatcatt aggacgtgtc gatcgtcgtc cctccccgcg
     1621 gcacgcgggg ttccgaattc cccgggttcg cgcgcgcgct catgcgggtg atgcagggtc
     1681 cctttcaaat ggcattccgg cgatttggtg atcggatgcg cgtacagggc cgcccgctcg
     1741 tcgagctcga gacggtcggc gccaaatcgg ggtcacgacg ccacgctgtg gtgggttcgt
     1801 ttcctgagat cgcggccgcg gtatcttcca gctgggtcgt ggtggcctcc aactctggat
     1861 cggcgcgtca tcccgcctgg ttcttaaatc tggcgaagaa gccggatcag gtctggatca
     1921 cgaccggcaa gcgccgagtc aaggtcaggc ccgagacgct ggagggcgtg gagcgcgaac
     1981 gcgcatggcg ggagatcact tcgctcgcac ccgggtacgg tcgctacgtc gagctcaccg
     2041 atcgcgtgat cccgatcgtt cgtctgagac aggagacgaa agaggcgtga cgccttcagt
     2101 ggcgcgcctc gggtgccgat cgatatttca gttcgaagcg aggactcgat cggcagggcg
     2161 caacgcccaa gagacgagtg tgagcagcat gacgacgatc ggatagcaaa gattgatgag
     2221 atc
//
LOCUS       DASPZD010000325         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1176480, whole genome shotgun sequence.
ACCESSION   DASPZD010000325 DASPZD010000000
VERSION     DASPZD010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176480"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1524
                     /locus_tag="VEM33_06355"
     CDS             40..1524
                     /locus_tag="VEM33_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYS51481.1"
                     /translation="MNIAATGINFIHQAEVAGRLRGLLPADAVLHAEEDTRSYECDGL
                     SAYRQLPMVVALPSSEEEVRQVLALCHELKAPVVARGAGTGLSGGALPMGDGVLLSLA
                     KMRRILALDPVARTARVQPGVTNLAISDAAAPCGLFYAPDPSSQIACSIGGNVAENSG
                     GVHCLKYGLTLHNVLRVRAVTVEGEVVEFGGEALDSPGLDLLALMIGSEGMLAVATEI
                     TVKLLPRPETARVILASFDDVEKAGNAVAEVIAAGVVPSGLEMMDRPAARAVEEFVHA
                     GYDLEAAALLLCEADGTPEEVEEGIAGMTGVLRRAGATAIRVSSSEVERQKFWAGRKA
                     AFPAVGRISPDYYCMDGTIPRRKLGDMLKFIAEMEKKYGLRCPNVFHAGDGNLHPLIL
                     FDANDPDQLERTERFAGEVLEKCIEVGGTITGEHGVGVEKINQMCVQFARAEIERFRG
                     VKRAFDPEGLLNPGKNIPTLHRCAEFGRMRVKGGQLPHPDLPRF"
     gene            1525..2304
                     /locus_tag="VEM33_06360"
     CDS             1525..2304
                     /locus_tag="VEM33_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51482.1"
                     /translation="MRAGSLASALMLGAGLCWRPDALAIVDEIQVYTDDLDYRGESGL
                     ELHLNTTPSGRRTPDYAGDVPPYHGVRITPEFSWGLGNDLDWGLYLPTATDSDWHTHL
                     GGAKLRVKWLPVHGAEGQGGWYFGINNELSSLTKAFSDSRWNDEVRVIGGYRGAGWLI
                     GVNPILGWGLSPGFRGSPDVTLAWKAMHEVAQGFVLGAEYYDDIGTLANRLPREEQSR
                     TLYAVMEIERKTWSLNFGIGHGLTPATDNWTVKAIVGISFN"
     gene            2330..>2862
                     /gene="glcE"
                     /locus_tag="VEM33_06365"
     CDS             2330..>2862
                     /gene="glcE"
                     /locus_tag="VEM33_06365"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HYS51483.1"
                     /translation="MDPVLAKLSETVRAAGGARRALRLRGGGTKDFYGQALEGEVLDT
                     RSYNGIVAYEPSELVITARCGARLGEIESAMNEHRQMLAFEPPHFGEGATLGGMIAAG
                     LSGPRRQAAGALRDFVLGVKIMDGRGEVLAFGGQVMKNVAGYDVSRLMAGSLGTLGII
                     LEVSLKALPRPAAEATL"
BASE COUNT          470 a          928 c         1006 g          458 t
ORIGIN      
        1 gcttggcgtg ggcgaagaaa tacttgtaag ctggacggga tgaacatcgc ggcgactgga
       61 atcaacttca tccaccaggc ggaggtcgca gggcgcctgc gcgggctgtt gcccgccgat
      121 gccgtgcttc acgcggagga agacacacgc tcctacgaat gcgacggcct ctcggcctac
      181 cggcagctgc cgatggtcgt ggcgttgcct tcgagcgagg aggaggtgcg gcaggtgctc
      241 gccctctgcc acgagctcaa agcgccggtc gtcgcccggg gcgccggcac cggcctctcg
      301 ggcggcgcgc tccccatggg ggacggagtg ctgctgtcgc tagcgaaaat gcggcgcatc
      361 ctcgcgctcg acccggtggc gcgtacggcg cgcgtgcagc ccggggtcac caatctcgcg
      421 atctcggatg ctgcggcgcc ctgcggcctt ttctatgcgc ccgatccctc gtcgcagatc
      481 gcctgctcga tcggcggcaa cgtcgccgag aactcgggcg gcgtgcattg cctgaaatac
      541 ggcctcacgc tgcacaacgt cctgcgggtg cgcgcggtca cggtggaggg cgaggtggtc
      601 gagttcggcg gcgaagcgct cgactcgccc gggctcgacc tgcttgcgct catgatcggg
      661 tccgagggca tgctcgcggt cgcgaccgag atcacggtga agctgctgcc ccgtcccgag
      721 acggcgcggg tcatcctcgc ctcgttcgac gacgtggaga aggcgggcaa tgcggtggcc
      781 gaggtcatcg cggcgggcgt cgtgccttcc ggcctggaga tgatggaccg gccggccgcg
      841 cgcgcggtgg aagaattcgt gcacgccggt tacgatctcg aagcggccgc gctcctcttg
      901 tgcgaggcgg acggcacgcc cgaggaggtc gaggagggca ttgccggcat gacaggggta
      961 ctccggcgcg caggcgcgac cgccatccgc gtctcgagct ccgaggtgga gcgccagaag
     1021 ttctgggccg gtcgcaaggc ggcatttccc gcggtgggac gcatctcgcc cgactactac
     1081 tgtatggacg ggaccattcc gcgaaggaaa ttgggcgaca tgctgaaatt catcgcggag
     1141 atggagaaga agtacggcct tcgctgcccc aacgtgttcc atgccggcga cggcaacctg
     1201 catccgctca tcctcttcga tgcgaacgac cccgaccagc tcgagcgcac cgagcgcttc
     1261 gccggtgaag tgctggagaa gtgcatcgag gtcggcggca ccatcaccgg cgagcacggc
     1321 gtgggcgtgg agaagatcaa ccagatgtgc gtgcagttcg cgcgagcgga gatcgagcgc
     1381 ttccgggggg tgaaacgcgc cttcgacccc gagggcctcc tcaatcccgg caagaacatc
     1441 ccgaccctgc accgctgcgc cgagttcggc cgcatgcgcg tgaaaggcgg gcagctgccg
     1501 caccccgatc tgccgagatt ctaggtgcgc gccggttcgc tggcctcggc cctgatgctg
     1561 ggcgccggct tgtgctggcg gcccgatgcg ctcgcgatcg tcgacgagat ccaggtgtat
     1621 acggacgatc tcgattaccg cggtgaaagc ggcctggagc tccacctgaa tacgacgccg
     1681 agcggtagaa ggacgccgga ctacgccggc gacgtccccc cttaccacgg cgtccggatc
     1741 acgccggaat tctcatgggg cctcggcaac gacctcgact ggggcctgta tctcccgacc
     1801 gcgaccgatt ccgactggca cactcatctc ggcggggcga agctgcgcgt gaaatggctg
     1861 ccggtgcacg gcgcggaagg ccagggcggc tggtacttcg ggatcaacaa cgaactctcc
     1921 agcctcacga aggccttctc cgactcccgc tggaacgacg aggtgcgcgt catcggaggt
     1981 taccgcggcg ccgggtggct gatcggagtg aaccccatat tggggtgggg gctctcaccc
     2041 gggttccgcg gcagccccga cgtcaccctc gcctggaagg cgatgcacga ggttgctcaa
     2101 gggtttgtgc tcggcgcgga gtattacgac gatatcggca cgctcgcgaa ccggctgccg
     2161 cgcgaggagc agagccgcac gctgtacgcg gtgatggaaa tcgagcgcaa gacctggtcg
     2221 ctcaatttcg gcatcggcca cgggctgacg cctgccaccg ataactggac agtcaaggcg
     2281 atcgtcggaa tttccttcaa ttgagactgc ggtgatactg tccggcccta tggatccggt
     2341 gctcgcgaag ctgtccgaga cggtgcgcgc ggcaggcggc gcgaggcgcg cgctgcgcct
     2401 gcgcggcggc ggcaccaagg atttctacgg tcaggctctc gaaggagagg tgctcgacac
     2461 gcgcagctac aacggcatcg tcgcctacga gccctcggag ctcgtgatca ccgcgcgctg
     2521 cggcgcgagg ctcggcgaga tcgaatccgc aatgaacgaa caccgacaga tgctcgcttt
     2581 cgagccgccg catttcggcg aaggcgccac gctaggcggc atgatcgcgg cggggctttc
     2641 cggcccgcgc cgccaggcgg cgggcgcgct gcgcgatttc gtgctcggcg tgaagatcat
     2701 ggacgggcgc ggggaagtgc tcgccttcgg cgggcaggtc atgaaaaacg tcgcaggcta
     2761 cgacgtctcg cgcctgatgg cggggtcgct cggcaccctc ggaatcatcc tggaggtgtc
     2821 cctcaaggcg ctgccccgcc ccgcggccga ggcgacgctc ag
//
LOCUS       DASPZD010000326         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1378661, whole genome shotgun sequence.
ACCESSION   DASPZD010000326 DASPZD010000000
VERSION     DASPZD010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378661"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /gene="fusA"
                     /locus_tag="VEM33_06370"
     CDS             complement(<1..1634)
                     /gene="fusA"
                     /locus_tag="VEM33_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYS51484.1"
                     /translation="MSNSIERIRTIALVGQSGAGKTTLAEALLAKAGAIPAAGSVERG
                     TTVCDYTPLEKQLQHSLKLAVASLDARTNGEATRIHLLDTPGYPDFLGQSLPALAAVE
                     TAAIVINAQNGIEMMTGRFMHWAAKRGLDRLVIVNKIDADNVDLEDLLERIQQTFGKE
                     CLPLNLPAGDRKKVSDCFFAPSGEADFSSVDEAHRKLVDQVVEVDEKLMEAYLEKGEV
                     TPEELHAPLERALREGHLIPVVFASAKTGAGIGELMNIIVQLLPNPLEGNPPVYVKTA
                     ADGKASELGAEPDPKLHALAHVFKIEIDPYIGRLAVFRVHQGRLTPGGQLYIGEGRKP
                     IKAGHLFLLRGKTQLEVNEALPGDICAIAKIDEIQFDHVLHDSAEDAQVHARPLELPT
                     SVFGLAVQPKKRGDEQKVSDVLHKITAEDPCFRIEHNSQANETVMRGLGEMHLRTVLE
                     KMSSQYKLDLDTRPPSIPYRETIAVRAEGHSRHKKQTGGAGQFGEVYLRIEPVDRGKG
                     FEFADEIFGGTIPGQFVPAVEKGVRDLLEQGPLAGYP"
     gene            complement(1694..>2204)
                     /locus_tag="VEM33_06375"
     CDS             complement(1694..>2204)
                     /locus_tag="VEM33_06375"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HYS51485.1"
                     /translation="SQVQARGHAEETRRPAGEKSRHPMTFEPHVPEISAVIQLVVAPV
                     FLLTAIGTIIAALNIRLGRSVDRRRELEGMLAHMSEAELPSAREELQTIARRMRDVYF
                     SILFAVISALFVCLLISTAFLGAILKTDLSYTIGGLFVSAMLALVICLLLFLREIFLA
                     VSTPRYIPR"
BASE COUNT          348 a          697 c          755 g          404 t
ORIGIN      
        1 gggtagccgg cgagcgggcc ctgttcgagc agatcgcgga cgcccttttc gacggcgggg
       61 acgaattgcc cggggatcgt gccgccgaag atctcgtcgg cgaattcgaa gcccttgccg
      121 cggtcgacgg gctcgatccg gaggtagacc tcgccgaact gcccggcgcc gccggtctgc
      181 ttcttgtggc ggctgtggcc ctccgccctc accgcgatcg tctcgcggta cgggatcgac
      241 ggcgggcgcg tgtccaggtc gagcttgtac tgcgacgaca tcttctcgag cacggtcctc
      301 aggtgcatct cgccgagccc gcgcatcacc gtttcgttgg cctgcgagtt gtgctcgatg
      361 cggaagcacg ggtcctcggc ggtgatcttg tggagcacgt cggaaacctt ctgctcgtcg
      421 ccgcgcttct tcggctgcac cgccagcccg aacaccgagg tcggcagctc gagcggccgc
      481 gcgtgcacct gcgcgtcctc ggcggagtcg tgcagcacgt ggtcgaactg gatctcgtcg
      541 atcttggcga tggcgcagat gtcgcccggc agcgcctcgt tcacctcgag ctgtgtcttt
      601 ccgcgcagca ggaacaggtg cccggccttg atcggcttgc gtccttcgcc gatatagagc
      661 tggcccccgg gggtcaggcg cccctgatgc acgcggaaca ccgcgaggcg cccgatgtag
      721 gggtcgatct cgatcttgaa gacgtgggcg agggcgtgca gcttcgggtc gggctcggcc
      781 ccgagctcgc tcgctttccc gtcagccgcc gtcttgacat agaccggcgg gtttccctcc
      841 agcgggttgg gaagcagctg cacgatgatg ttcatcagct cgccgatgcc cgctccggtc
      901 ttcgccgagg cgaacaccac cgggatgaga tgcccctcgc gcagcgcccg ctcgagcggc
      961 gcgtgcagct cctcgggcgt cacctcgccc ttctcgagat aggcctccat cagcttctcg
     1021 tcgacctcga ccacctggtc gaccagcttg cggtgcgcct cgtccacgga cgagaagtcg
     1081 gcctcgcccg aaggagcgaa gaagcagtcg gagaccttct tgcggtcgcc ggcgggcagg
     1141 ttgagcggca ggcactcctt gccgaacgtc tgctggatgc gctcgagcag gtcctccagg
     1201 tcgacgttgt cggcgtcgat cttgttgacg atgaccagcc ggtcgaggcc gcgcttcgcc
     1261 gcccaatgca tgaagcgtcc ggtcatcatc tcgatgccgt tctgcgcgtt gatgacgatg
     1321 gcggcggttt ccaccgccgc cagggccggg agcgactggc cgagaaaatc ggggtagccg
     1381 ggggtgtcga gcaggtggat gcgggtggct tcgccattgg tcctggcgtc gaggctcgcg
     1441 accgcgagct tcagggagtg ctgcagctgc ttctcgagcg gggtgtaatc gcacacggta
     1501 gtgccgcgct cgacgctgcc ggccgcgggg atcgcccccg cttttgcgag cagcgcctcg
     1561 gccagcgtgg tctttcccgc tccggactgg ccgacgagcg caatggtcct gatccgttcg
     1621 attgaatttg acacgagcct gcctcctcgc gccgatttat agcagcatcg cgcggggctg
     1681 gggaagagcc ccgttagcgg gggatgtagc ggggcgtcga cacggcgaga aaaatctcgc
     1741 gtaaaaacaa gagcaggcag atgaccaggg ccagcatcgc ggacacgaac aggccgccga
     1801 tggtgtagga aaggtcggtc ttgaggatag cgcccaggaa cgcggtggag atcagcaggc
     1861 agacgaaaag cgccgagatc accgcgaaca gaatggagaa atagacgtcg cgcatgcgac
     1921 gtgcgatggt ctgcagctct tcacgcgccg aaggcagctc ggcttcgctc atgtgcgcca
     1981 gcattccttc gagctcgcgg cgccggtcga cggagcggcc caggcggatg ttgagcgcgg
     2041 caatgatggt cccgatcgcg gtcagcagga ataccggcgc aacgacgagc tggatcaccg
     2101 ccgagatttc aggaacgtgc ggttcgaaag tcattgggtg cctgcttttt tctccagcag
     2161 gtcgccgcgt ttcttcagcg tgtcctcgag cttggacttg ggaa
//
LOCUS       DASPZD010000327         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_491701, whole genome shotgun sequence.
ACCESSION   DASPZD010000327 DASPZD010000000
VERSION     DASPZD010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491701"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VEM33_06380"
     CDS             complement(<1..200)
                     /locus_tag="VEM33_06380"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYS51486.1"
                     /translation="MIRAFSRDHAALSLSDIARSARIPAATARRCLLTLEELGYVTRS
                     ARDFLLRPKVLELGAAYLESMN"
     gene            400..1212
                     /locus_tag="VEM33_06385"
     CDS             400..1212
                     /locus_tag="VEM33_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HYS51487.1"
                     /translation="MKIGRQEQRSSAISTSNEQTILVRGRDLTRELIGRITFTEHFWL
                     LVTGSLPTPAQLRVLDATLVAIAEHGLVPSVQVSRMTLAAAPEALQGAVAAGILGCGS
                     VVLGSSEAAGRFLAEISARGKSAASLEAAAREVVREYREAKQAIPGYGHPLHRGGDPR
                     AQRLLEVAVEANTSGHHAEIARVVERLLPEVIGRPLALNVSGAIPAVLLDAGYPLLAL
                     KGVPLLARTAGLVAHLLEEQQRAIGFVMSHAAAQAINYDGEAPPDFKPSRDG"
     gene            1239..2396
                     /locus_tag="VEM33_06390"
     CDS             1239..2396
                     /locus_tag="VEM33_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HYS51488.1"
                     /translation="MAGIRVVEQGTFITGPCAGMMLADLGADVIKVESPEGDPYRAYQ
                     GGQYSPHFQAYNRNKRSVALDMKQSADCELFRGLIREADVYIQNFRPGTAERLGAGVE
                     RLLQINPRLVYCSISGFGASGPYAERPSYDSVAQALSGFLSVVVDYRRPQFLGPALAD
                     AITGLYASQGILAALVQRGRSARGGHVEVSMLEAMAHFAVEPFAAFFALGQTPTSADR
                     PRLAQAYILRTADERLIAIHLSSLEKFWQGLVAALGAPELASDPRFNPRERRVAEYEA
                     LRLELDARFVREPLSHWIGRLQANDVPYAPVNRIDDVVADPQIRHLGIVVPVEAPHSA
                     KQAVRPALQFNGNRAKSVSAAPLLDQHGKQIRAALAGGPTWPPGETSALVS"
     gene            2420..3481
                     /locus_tag="VEM33_06395"
     CDS             2420..3481
                     /locus_tag="VEM33_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYS51489.1"
                     /translation="MLFPTTLVGSYPQPEWLIDRERLAARFPPRVRARELWRVPEAFL
                     AQAQDDATIMAIKAQEDAGLDIITDGEIRRESYSNRFATALDGIDLDNPGTALDRSGH
                     PNPVPRVVAPIRRRHAVGVRDLEFLRSHTRRDVKATVPGPFTMAQQAQIDYYGGSREH
                     AAMDYAAAVNEEIRDLFDAGADVVQIDEPYMQARPEEARAFGLRAVNRALEGISGVTA
                     VHICFGYAAIIHVRPSGYSFLPELAQCRCAQVSIETAQSSLDCAVLKALPGKKIILGV
                     IDLGTHAIETPEVVANRIRRALPFVDPANLLVAPDCGMKYLPREVAFGKLQAMVAGAQ
                     IVRLQLAAERTGPGGGAKS"
     gene            3506..>4222
                     /locus_tag="VEM33_06400"
     CDS             3506..>4222
                     /locus_tag="VEM33_06400"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYS51490.1"
                     /translation="MFAPLRRLLPLLAALPGVVLFTAPAWGQSQATALEEVVVTARKR
                     DETIQNVPVTENVFTQQAIEAAGIESPRDFVAMVPNMTLVEVQNVGNSFITIRGISQA
                     RNSEPSVAVLVDGVLETNPYQFDQELTDIREIEVLKGPQGALYGRNAIGGAILIHTVD
                     PSDHFAGAARVGVGNGVSEKAQVAVSGPIDSAGTLKYRASLNYYNTDGYRQNALLDRK
                     ADPYRDYSGRLRLLWKPGHQW"
BASE COUNT          715 a         1409 c         1399 g          699 t
ORIGIN      
        1 atattcatcg attccaggta tgccgcgccg agctcgagca ctttcgggcg cagcagaaaa
       61 tcacgcgcgc tgcgcgtgac gtagccgagc tcctccagcg ttagcaggca gcgtcgtgcc
      121 gtggcggccg gaatgcgggc actgcgcgcg atgtcactga ggctcaaggc cgcatgatcg
      181 cgcgaaaagg cgcggatcac ggcgagtccc ttagcgagtc cgcccatcgc ctcacgacga
      241 ggcggcgttt tcctaccctt tggcacgtac gcagtctatt tcgatatccg aaatgtcgga
      301 agggcgcgga gcagccttga cgagggtcca ggggcaagtc cactattgtt tcggataccg
      361 acgtcgattt cggtatgcga aatacacttg gggggccact tgaagattgg gcggcaggag
      421 caacgcagct cggcgatttc gacctcaaac gaacagacca tcctcgtccg ggggcgcgat
      481 ctgacgcgtg aactgatcgg caggatcacc ttcaccgagc acttctggct gctggtgacc
      541 ggttcgctgc ccacgcccgc tcagctgcgc gtgctcgatg caacgctggt ggcgatcgcc
      601 gaacacgggc tggtgccgag cgttcaagtc tcccgcatga ccctggcggc ggcgcccgag
      661 gctctgcagg gagcggttgc ggccgggatt cttgggtgcg ggtcggtggt cctcggctcc
      721 tctgaggccg cgggtcgttt ccttgcggag atcagtgccc gtgggaaaag cgctgcgtca
      781 ctcgaggcag ctgcccgcga ggtagtgcgc gagtaccgcg aggccaagca ggcgatccct
      841 ggctatggcc atccgctgca ccggggcggt gatccgcgcg cacagcgcct gctcgaggtg
      901 gccgtcgagg cgaatacctc ggggcaccac gcggagattg cccgcgtcgt cgagcggctc
      961 ctgccggaag tcatcggccg tccgctcgcg ctcaacgtct ccggagcgat tccggcggtg
     1021 ctcctggatg ccggttatcc gctgctggcc ctgaagggtg tgccgttgct tgcgcgcacc
     1081 gcggggctcg tcgcgcatct gctcgaggaa cagcagcgcg ccatcggctt cgtcatgtca
     1141 cacgccgcgg cgcaggcgat caactacgac ggtgaagccc ctcccgactt taagccgagc
     1201 agggacggtt gaggcaccgc acagctatgg aaccgacatt ggcgggcatt cgcgtcgtcg
     1261 agcagggcac gttcatcacc gggccctgcg ccgggatgat gttggcggac ctcggcgcgg
     1321 acgtcatcaa ggttgagagc cccgaggggg acccgtaccg cgcctaccag ggcggccagt
     1381 actcgccaca ctttcaggcc tacaaccgca acaagcgcag cgtcgcgctc gatatgaagc
     1441 agagcgcgga ctgcgaactc ttccggggcc tcatccgtga ggccgacgtg tacatccaga
     1501 acttccggcc cgggactgca gagcgcctcg gagccggtgt cgaacgactg ctgcaaatca
     1561 acccgcggct cgtgtactgc tccatcagcg gcttcggcgc gagcggcccg tacgccgagc
     1621 gcccgagcta tgactccgtc gcgcaggcgc tcagcgggtt tctcagcgtg gtagtcgact
     1681 accgccgacc acagttcctg ggtcccgcgc ttgccgatgc aatcacgggt ctttatgcct
     1741 cacagggcat cctcgcagcc ctggtgcaaa ggggccgcag cgcgcgcggc ggtcacgtcg
     1801 aagtatccat gctggaagcg atggcgcact tcgccgtcga acccttcgcc gcgttcttcg
     1861 cgctcggaca gactcctacc tccgccgacc ggccgcggct ggcgcaggcc tacatcctgc
     1921 gcacggcaga cgagcggctg attgcgattc acctgtcgtc gctcgagaag ttctggcagg
     1981 gactggtcgc ggcgctcggg gcaccggagc ttgcgagtga tccacgcttc aacccgcgcg
     2041 agcgccgcgt cgccgagtat gaggcgctgc gtctggaact cgacgcgcgt ttcgtgcgcg
     2101 agccgctgtc gcactggatc gggcgactgc aggccaatga cgtgccctac gcacccgtca
     2161 accgcatcga tgacgtggta gcggacccac agatccgcca tctcggcatc gtcgtgccgg
     2221 tcgaagcccc gcactcggca aagcaagccg tgcggccggc actgcagttc aacggcaacc
     2281 gggccaaatc ggtgagtgcc gcaccgcttc tggatcagca cggcaagcag atccgcgctg
     2341 cactggccgg cgggccgaca tggccccccg gcgagacgag cgcactggtc agctaggcgc
     2401 gcgcaaaggg gaaaccgcga tgctctttcc aactacgctc gtcggcagct atccgcagcc
     2461 ggagtggctg attgaccgcg agcgcctcgc cgctcgcttc ccgccgcggg tgcgggcgcg
     2521 cgaactgtgg cgtgtcccag aggcgttcct ggcgcaggcg caggatgacg cgaccatcat
     2581 ggccatcaag gcacaggagg acgccgggct cgacatcatc acggacgggg agatacggcg
     2641 cgagagctac tccaaccgct tcgcgaccgc cctcgatgga atcgatcttg acaatccggg
     2701 caccgcgctc gatcgcagtg gtcacccgaa ccccgtgccg cgtgttgtcg cgccgatccg
     2761 ccgccgccat gcggtcggcg tgcgcgacct cgagtttctg cgcagccaca cccggcgcga
     2821 cgtcaaggcg acggttccgg ggccgttcac gatggcccag caggcgcaga tcgactacta
     2881 cggcggaagc cgcgagcacg ccgccatgga ctatgcggcc gccgtgaacg aggagatccg
     2941 cgatctcttc gatgccggtg cggatgtcgt gcagatcgac gaaccctata tgcaggcgcg
     3001 cccggaggaa gcacgtgcgt tcggcctgcg tgcggtcaac cgcgcgcttg agggcatctc
     3061 cggggtcacg gccgtgcaca tctgcttcgg ctacgcggcc atcattcacg tgcgcccgtc
     3121 cggctattcg ttcctgcctg agctcgcgca gtgccgctgc gcgcaggtct cgatcgagac
     3181 cgcgcagtcg agcctcgact gcgccgtgct caaggcgttg cccgggaaga agatcatcct
     3241 cggcgtcatc gacctgggta cccacgcgat cgagacgccg gaggttgtgg cgaaccggat
     3301 ccgccgcgcg ctgcccttcg tcgatccggc gaatctcctg gtcgcacccg attgcggcat
     3361 gaaatacctg ccgcgcgagg tcgccttcgg gaagctgcag gcgatggtgg ccggcgcgca
     3421 gatcgtgcgg ctgcagcttg cggccgagcg gaccgggccg ggcggcggcg ccaaatccta
     3481 acaatcgata cagagggggg cttatatgtt cgcaccgttg cgcagattac ttccactcct
     3541 ggcggccttg ccgggcgtag tcctgttcac cgcccctgcc tgggggcaaa gccaggccac
     3601 ggcgctcgag gaagtggtgg tcaccgcacg taaacgcgat gagacgatcc agaacgtgcc
     3661 ggtgaccgaa aacgtcttca cccagcaggc gatcgaggcg gcgggaatcg agtctccccg
     3721 cgacttcgtc gcgatggtcc cgaacatgac cctggtcgag gtgcagaacg tcggcaactc
     3781 attcatcacc atccgcggca tctcccaggc ccgcaacagc gagccgtcgg tcgcggtgct
     3841 ggtcgacggc gtgctggaga ccaatcccta ccagttcgac caggagctga ccgacatccg
     3901 ggagatcgag gtgctgaagg gcccgcaggg cgcgctctac gggcgcaatg ccatcggcgg
     3961 tgcgatcctc attcacaccg tggatccttc cgatcacttt gccggcgccg cccgggtcgg
     4021 cgtgggcaac ggcgtgtccg agaaggcaca ggtggccgtc agcggcccga ttgactcggc
     4081 gggtacgctc aagtatcgcg cctcgctcaa ctactacaac actgacggct atcggcagaa
     4141 cgcgctcctg gaccgcaagg cggacccgta tcgggattac tccgggcggc tgcggctgct
     4201 gtggaaaccc ggccaccaat gg
//
LOCUS       DASPZD010000328         4104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_761712, whole genome shotgun sequence.
ACCESSION   DASPZD010000328 DASPZD010000000
VERSION     DASPZD010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761712"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..906
                     /locus_tag="VEM33_06405"
     CDS             190..906
                     /locus_tag="VEM33_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51491.1"
                     /translation="MRRLARLCSLSLLLSQGLVAHAEPYNLGSFRLAVIDDPWRIELQ
                     GDKPVTRQDFEQVVKIVGATRGWKVANSTEGRMELTNVIREHTMSVELTYSATGYGIR
                     YLQSINLLYEERGGGMLRLIHGNYNGWIKDLVSGINAGLGVPAQTTIGFAPIQKVDAV
                     PFLGERGRDAYKEFLSKPTPRAFAIAPNGAWASSSRTAPGSRQQLDVVGYAMESCNRR
                     GNGECKLYALDERVVWRSDR"
     gene            911..1462
                     /locus_tag="VEM33_06410"
     CDS             911..1462
                     /locus_tag="VEM33_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HYS51492.1"
                     /translation="MVSLIRRRLLAAAAALPVLPAFAQTRPLTCGELTSSQTEGPFFK
                     TNTPRRSSLVEPQSKAQRLIVTGQVLSAQCRPVANALLDFWHADEDGEYDNRGFRYRG
                     HQFTDAEGRYRLQTIVPAEYPGRARHLHVKVQAPGKRILTTQLYFRDEPGNRRDGLYR
                     PDLEMRMAAKGAGEGRFDFVVEV"
     gene            complement(1479..2687)
                     /locus_tag="VEM33_06415"
     CDS             complement(1479..2687)
                     /locus_tag="VEM33_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51493.1"
                     /translation="MTRITCSLLALAAVTVASIASAQEPIRIGVLNDQSSVYADFQGR
                     GSVLAAQMAVEDYGGKAAGRKVEVVFADHQNKPDVGSVVARKWLDQDGVDLIVDVPNS
                     AVAFGVSEIVRQKNKVLIGSGAGSSELTGSKCSPNTVHWTYDTWSYGHSLARAVVQRG
                     GKTWFFITADYAFGHDLEKQATDQLLKDGGKVLGGVRTPIATPDFSSFLLAAQSSGAQ
                     VVALAVAGGDTTNSMKQAAEFGLPARQSIVSLIFGINNVPALGLKASQGALTVNPFYW
                     DMNEGTRAWSQRFRKRAHNAAMPNDMQAGVYASVLHFLKAVDKAGGAGDGRTVVAAMK
                     AMTTDDPLFGKGRIREDGRKIHPMYLLQVKSPEESKGDWDYFKIVASIPAEQAFRPLN
                     EGNCPLVAGK"
     gene            complement(2701..>4104)
                     /locus_tag="VEM33_06420"
     CDS             complement(2701..>4104)
                     /locus_tag="VEM33_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYS51494.1"
                     /translation="VSPAYSLMSKDFAKLKAIFELLHPGLVWTADPAKFAPALAAVGA
                     RPTSIDELLPAKAGSRVDEAFAGIGPGTVAKILFTSGSTGIPKGVINTQRMLTVNQEQ
                     YAFVWPFLEDRPQVLVDWLPWNHTFGGNNNFGMMLRNGGTFHIDGGKPAPGLIDITAA
                     NLKEVSPTIYFNVPRGFDLLLPFLEKDASLRRSFFRDLDCMVYAGASLPQNLWERMQQ
                     LSVTELRRRLPLISAWGSTETAPMATGVHYAVDRAGIVGLPVPGCELKLLPAAGKLEA
                     RVKGPNVTPGYWGRDDLTKAAFDEEGYYRIGDALKFADPTKPELGLAFDGRIAEDFKL
                     STGTWVHVGATRVKLIAAGDPVIQDAVITGHERSEVGALVFLNAAAVKVKGLDDAGVC
                     EHLRAAMRRLAAEAGTGSSTHPVRALVMSEPLSIDANEITDKGYINQSAVLERRAALV
                     EKLHTDSPASEVILAGH"
BASE COUNT          713 a         1335 c         1332 g          724 t
ORIGIN      
        1 ctcctggtcg acgacgccga gatgatgaag cgcaagcgcg cgtggaaggc gcccgcgcgc
       61 aggttcgagc gcggctacgg cgcgctcttc tcgcagcaca tccgccaggc gaacgaaggc
      121 tgcgacttcg atttcctcga aggcaccgcg ccgacgccgg agccggagat tcactgaggg
      181 agggtgacta tgaggcgact ggctcgtctg tgttcccttt ctctgttgtt gtcgcagggc
      241 ctcgtcgcgc atgccgaacc ctacaacctc ggatccttcc gcctggcggt gatagacgat
      301 ccctggcgga tcgaacttca gggcgacaag ccggtgaccc ggcaagactt tgaacaggtc
      361 gtcaagatcg taggggcgac ccggggttgg aaggtagcca actcgaccga gggccgcatg
      421 gagctgacga acgtcatacg cgagcacacg atgtcggtcg agttgaccta cagcgcaacc
      481 ggctacggca ttcgatattt gcaaagtatc aatcttctat atgaagaacg cggcggcgga
      541 atgcttcggt tgattcatgg caactacaac ggctggatca aggatctcgt atcggggatc
      601 aatgcaggcc tcggagtgcc ggcgcaaacg acgatcggct ttgcgccgat ccagaaagtc
      661 gacgccgttc cctttctcgg ggagagggga cgcgacgcct acaaggaatt tctgtccaag
      721 ccgacgccgc gggcgtttgc gatcgcgcca aacggagcat gggcatccag ctcccggacc
      781 gctcccggga gcaggcagca gctcgacgtc gtcgggtacg cgatggaaag ctgcaatcgt
      841 cgtggaaacg gggagtgcaa gctctacgcc ctggacgagc gcgtggtgtg gcgctccgac
      901 cggtgaagca atggtctctc tgattcggcg ccggctcctt gccgccgcgg ccgcgctccc
      961 ggtccttccg gcctttgctc agacgcgccc gctcacctgc ggcgagctca cgtcttcgca
     1021 gactgagggc ccgtttttca aaaccaatac gccgcgccga agttccctgg tagagccgca
     1081 atccaaggcc cagagactca tcgtgaccgg gcaggtcttg tcggcccagt gcagaccggt
     1141 cgcgaacgcg ctgctggatt tctggcacgc agacgaggac ggcgagtacg acaaccgcgg
     1201 ctttcgctat cgcggccacc agttcaccga cgcggagggg cgttatcgcc tgcagaccat
     1261 cgttccggcg gagtatcccg ggcgcgcgcg ccatctacac gtcaaggtgc aggcgccagg
     1321 aaagcgcatt ctcacgacac aactctactt ccgggacgag cccggcaacc gccgcgacgg
     1381 cctttaccgc ccggacctcg agatgcgcat ggccgcaaag ggcgccggcg agggaaggtt
     1441 cgacttcgtc gtcgaggtct gaagtcgggg acgcgagatc atttccccgc gaccagcggg
     1501 caattgccct cgttcagggg gcggaacgcc tgctccgccg gaatgctggc cacgatcttg
     1561 aaatagtccc agtcgccttt cgactcctcg ggcgatttca cctgcaggag atacatcggg
     1621 tggattttgc ggccgtcctc tcggatccgg cctttgccga acagcggatc gtcggtggtc
     1681 atcgctttca tcgcggccac cacggtcctg ccgtcgcctg cgccgcccgc cttgtcgacc
     1741 gctttgagga aatgcagcac ggacgcatac accccggcct gcatgtcgtt cggcatggct
     1801 gcgttgtgcg cgcgcttgcg gaagcgctgc gaccacgcgc gcgtgccctc gttcatgtcc
     1861 cagtagaacg ggttcaccgt gagcgccccc tgtgaagcct tgagccccag cgcgggcacg
     1921 ttgttgatgc cgaaaatcag agagacgatg ctttgcctgg ccggcagccc gaactccgcc
     1981 gcctgcttca tcgaattggt ggtgtcgccg cccgcgaccg ccagcgcgac cacctgggcc
     2041 ccggaagatt gtgcagccag caggaacgac gagaaatccg gcgtcgcaat cggcgtgcgc
     2101 acgccgccca agaccttgcc tccgtccttc agcagctgat cggtcgcctg cttctccagg
     2161 tcgtggccga aggcgtagtc ggcggtgatg aagaaccagg ttttgccgcc gcgctggacc
     2221 acggcccttg caagcgaatg cccgtacgac caggtgtcgt aggtccagtg cacggtgttc
     2281 ggcgagcact tcgatccggt gagctccgag cttcccgccc cggagccgat cagcaccttg
     2341 ttcttctgtc gcacgatctc gctgacgccg aacgccaccg ctgaattcgg gacatccacg
     2401 ataaggtcga cgccgtcctg atcgagccac ttgcgcgcca cgaccgagcc gacgtcgggc
     2461 ttgttctggt ggtcggcgaa aaccacctcg accttgcgtc ccgcggcttt gccgccatag
     2521 tcttcgaccg ccatctgcgc ggcgagcacc gagccgcgcc cctgaaaatc cgcgtacacg
     2581 ctcgattggt cgttcaggac gccgatacgg atcggctcct gcgcactggc gatgctcgcg
     2641 acggtgaccg ccgcgagggc gagcaagcta caggtgattc gagtcatgga atgctccctt
     2701 ctagtgaccg gcgaggatga cctcggaggc aggcgagtcg gtgtgaagtt tttcgacgag
     2761 cgccgcgcgg cgctcgagca ccgcgctctg gttgatgtag cccttgtcgg tgatctcgtt
     2821 cgcgtcgatc gagagcggct cgctcatgac gagcgcgcgc accgggtggg tggagctgcc
     2881 cgtgccggct tcggcggcaa gcctcctcat cgccgcgcgc aggtgctcgc acaccccggc
     2941 gtcgtcgagc cccttcacct tgaccgccgc agcgttcagg aacacgagcg cgccgacctc
     3001 gctgcgctca tggccggtga tcactgcgtc ctggatcacg ggatcgcccg cggcgatcag
     3061 tttcacgcgc gttgcaccca cgtgcaccca ggtgccggtc gaaagcttga agtcctcggc
     3121 gatgcgcccg tcgaaagcga ggcccagctc gggctttgtc ggatcggcga atttcagcgc
     3181 gtcgccgatg cggtagtagc cctcctcgtc gaacgcggcc ttcgtgagat cgtcgcggcc
     3241 ccagtagccg ggcgtgacgt tcgggccttt cacgcgcgcc tcgagcttgc ccgcggcggg
     3301 caggagcttc agctcgcagc cgggcaccgg cagcccgacg atgcccgcgc gatcgaccgc
     3361 gtagtgcacg cccgtcgcca tcggcgcggt ttcggtggaa ccccacgcgg aaatgagcgg
     3421 gagccggcgc ctgagctcgg tgaccgagag ctgctgcatg cgctcccaca ggttctgagg
     3481 gagggacgct ccggcgtaca ccatgcagtc gaggtcgcgg aaaaaactgc gccggaggga
     3541 cgcgtccttc tcgagaaagg gcaagagcag gtcgaagccg cggggcacgt tgaagtagat
     3601 ggtcggcgag acctccttca agttcgctgc cgtgatgtcg atgaggccgg gagcgggctt
     3661 gccgccgtcg atatggaacg tgccgccgtt tctcagcatc atgccgaaat tgttgttgcc
     3721 gccgaaggtg tggttccacg gcagccagtc gaccagcacc tgcggccggt cctcgagaaa
     3781 gggccagacg aacgcgtact gctcctggtt gacggtaagc atgcgctggg tgttgatcac
     3841 gcccttgggg attccggtcg aaccggaagt gaaaaggatc ttcgcgacgg tcccaggtcc
     3901 gattccggcg aaggcctcgt ccaccctgga gcccgctttc gccggaagca gctcgtcgat
     3961 cgacgtcggc ctcgcgccga ccgcggcgag cgcaggcgcg aacttcgcgg gatcggcggt
     4021 ccacaccagg cccggatgca gcagctcgaa gatcgccttg agcttggcga aatccttcga
     4081 catcagcgag taagcgggcg agac
//
LOCUS       DASPZD010000329         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1761891, whole genome shotgun sequence.
ACCESSION   DASPZD010000329 DASPZD010000000
VERSION     DASPZD010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761891"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VEM33_06425"
     CDS             complement(<1..326)
                     /locus_tag="VEM33_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51495.1"
                     /translation="MITLDRSTLVRAGVASFAVAVLSGSAFANQHPHPHKGSLDYLDR
                     NSYAKNMRVLGVFQLGEERGHKLQMMAIGSRRLILQNGDVIDVSDPRTPKLVKHAAFR
                     GSQLQVA"
     gene            complement(351..1724)
                     /locus_tag="VEM33_06430"
     CDS             complement(351..1724)
                     /locus_tag="VEM33_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYS51496.1"
                     /translation="MNAPAGIRSVAERLADATASLSSGGIPAQVRLRCEDLLVDVGGL
                     CVAARASEYVRALVASVDGGGPCTAIGHASGFRPEDAAMIGGTAAHGEDFDDTFEGGP
                     VHSGAVVVPAVLAACERFQRDGQAALRGIASGVETMCRLSLVAPKLIHKAGFHPTSVL
                     GAMAATLGVSAALGLDRRQMVDALGTAGSMAGGIIEYLAEGAWTKRLHAGWAARVGLH
                     AARIGREGFLGPRTVFEGTHGFFFGFAHTREGDFDALTRGFGRQWLMEGLTFKPYATG
                     TMNQPYIDCALRLARKGFAAEDVAEVLCETAEGYVHRLWEPLSSKHRPANDYAAKFSA
                     PFNIAVAFVTGSAGFEAFTEKTVRDPRILALASKVRYVVDPDNPYPKAYTGHVKMTLR
                     DGRVFEDRQPHIRGGVHEPLTREEIERKFRSNATYGGWDTARAERFLQFARNAFDKPV
                     DLSGFRG"
     gene            complement(1734..>2187)
                     /locus_tag="VEM33_06435"
     CDS             complement(1734..>2187)
                     /locus_tag="VEM33_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51497.1"
                     /translation="TGTRWAYISFPGGGERIAALLGGHVNIMVIEPEEAGEHIRAGNM
                     RVLAQISEKRLAKFPEVPTLKEAGFDVPVVPQVRGVVAPPGIPKENVEFWQDFFRRLS
                     RTPSWHKYIEDNQFEDGYQNAADLAKFYDEFTDRMRDIMKDAGVKTVR"
BASE COUNT          366 a          719 c          702 g          400 t
ORIGIN      
        1 gcgacctgta gctgcgaacc tctgaaggcg gcgtgtttca cgagcttggg cgtgcgcggg
       61 tcgctgacat cgatcacatc gccgttctgc aggatcaagc ggcgcgaacc gatcgccatc
      121 atctgcagct tgtggccgcg ctcctccccg agctggaaca ctccgagcac gcgcatgttc
      181 ttggcgtagc tgttgcggtc gaggtaatcg aggcttccct tgtgagggtg cggatgttgg
      241 tttgcaaagg cgctgccgga cagtacggcg actgcgaagg aggcgacgcc cgcgcgaaca
      301 agggtactgc gatcgagagt gatcatgaaa ttcttcctgt gagacggaca ctagccacgg
      361 aaacccgaca agtcgacagg tttgtcgaaa gcgttccttg cgaattgcag aaaccgctct
      421 gcgcgcgccg tatcccagcc gccgtacgtt gcgttggagc ggaacttgcg ctcgatctct
      481 tcgcgcgtga gcggctcatg cacgccgccg cggatatgcg gctggcggtc ctcgaataca
      541 cgcccgtccc gcagtgtcat ttttacgtgg ccggtgtaag ccttcggata cggattgtcc
      601 ggatcgacga cgtaacgcac cttggaagcg agcgcgagaa tgcgcggatc gcgcacggtc
      661 ttttcggtga acgcctcgaa cccggcgctg ccggtgacaa acgcgacggc gatattgaag
      721 ggcgcgctga acttggcggc gtagtcgttt gccggccgat gtttggatga aagcggctcc
      781 catagccggt gcacgtaacc ttcggcggtt tcgcacagca cctcggcgac gtcttcggcg
      841 gcaaagcctt ttctcgccag ccgcagcgcg caatcgatat aaggctgatt catggttccg
      901 gtcgcatacg gcttgaaggt gaggccttcc atcagccatt gcctgccgaa accgcgcgtg
      961 agggcgtcga aatcgccttc ccgggtgtgg gcaaacccga agaaaaatcc atgcgtgcct
     1021 tcgaatacgg tacgtggccc gaggaatcct tcgcgcccga tgcgcgccgc gtgcagcccg
     1081 acgcgcgcgg cccatcccgc gtgcaggcgc ttggtccagg ctccctcggc gagatattcg
     1141 atgatcccgc ccgccatgct gcccgccgtg ccgagtgcat cgaccatttg cctgcgatcg
     1201 agcccgagcg cggccgatac gccgagcgtt gccgccatgg ctccgagcac gctcgtgggg
     1261 tgaaatcccg ccttgtggat cagcttcggc gcgacgaggc tcaaccggca catcgtttcc
     1321 acgcccgagg cgatcccgcg aagcgcggcc tgcccgtccc gctggaagcg ctcgcacgcg
     1381 gcgagcactg ccgggacgac gactgctccg ctgtgcaccg ggccgccctc gaaagtgtcg
     1441 tcgaagtctt cgccgtgggc ggccgtgccg ccgatcatcg cggcgtcctc ggggcgaaag
     1501 ccgctcgcgt gcccgatcgc ggtgcaggga ccgccgccgt cgacgctcgc gacgagagcc
     1561 cggacgtatt cgctcgcgcg cgccgccacg cacaggccgc cgacgtcgac gagcaggtcc
     1621 tcgcaacgca gcctgacctg ggccggaata ccccctgacg acagcgacgc ggtcgcgtcg
     1681 gcgagtcgtt ccgcgacgga tcggatgccg gcgggagcgt tcatccggtc gattcagcgc
     1741 accgtcttga cgccggcgtc cttcatgatg tcgcgcatcc ggtccgtgaa ctcgtcatag
     1801 aatttcgcga ggtccgcggc attctgatat ccgtcctcga actggttgtc ctcgatgtac
     1861 ttgtgccagg acggggtacg cgacaatctg cggaaaaaat cctgccagaa ctcgacgttt
     1921 tccttgggaa ttccgggcgg cgcgaccacg ccgcgaacct gcggcacgac cggcacgtcg
     1981 aacccggcct ccttgagcgt gggcacttcg gggaatttcg ccagccgctt ctcgctgatc
     2041 tgcgcgagca cgcgcatgtt cccggcgcgg atatgctcgc ccgcctcttc gggctcgatc
     2101 accatgatgt tgacgtggcc tccgagcaag gcggcgatgc gctcgccccc gcccggaaac
     2161 gatatgtagg cccagcgcgt gcccgtg
//
LOCUS       DASPZD010000330         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1272719, whole genome shotgun sequence.
ACCESSION   DASPZD010000330 DASPZD010000000
VERSION     DASPZD010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272719"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3593)
                     /gene="rpoC"
                     /locus_tag="VEM33_06440"
     CDS             complement(<1..>3593)
                     /gene="rpoC"
                     /locus_tag="VEM33_06440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYS51498.1"
                     /translation="KALLDLFKQVTQEEEFDAIKTGLASPEKIRSWSYGEVKKPETIN
                     YRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLAKVRRERMG
                     HIELASPVAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVTDAGMVNDEKIKRAK
                     LLSEEDYLAKIEEHGDDFSASMGAEGIRELLRAINLNEQIDTLRKELESTGSDTKIKK
                     LAKRLKVLEGFQKSGIKPDWMVMEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKKMALELFKPFIFNKLEMQGI
                     ATTIKAAKREVEAETPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNILSPANGDPIIVPSQDI
                     VLGLYYATRERVNARGEAMVFADVTEVARAYDSGKVELHAGVTVRIREAEFGPGGEKI
                     EKLVRHRTTVGRALLSEILPVGLPFELINKALKKKEISKLINASFRRCGLRETVIFAD
                     KLMQAGFTIATRAGISICVDDMLVPTQKHDLITAAEGEVKEIETQYTSGLVTVGERYN
                     KVVDIWGRAGDQVGKAMMDQLSSEDVTDRKGQKVKQESFNSIYMMADSGARGSAAQIR
                     QLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVTEEDCRTTNGVAMKALVEGGEVVEALRERILGRVTASDLLHPETQ
                     EVLYNAATLLNEDEVENIEALGIDEVRVRTPLTCDTRWGLCAACYGRDLARGSSVNVG
                     EAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAANQVEAKSNGTVRFTPQIRYVTNP
                     KGEKVVISRTGEVLVTDDNGRERERHKVPYGATLLAADSKPVKAGQTLASWDPHTRPI
                     ITEHGGIVRFEHIEEGVTVAKQIDEVTGLSTLVVIDPKRRAAAGKGLRPQVKLLNESS
                     EEIRIAGTDHPITITFQVGSLITVRDGQQVQVGEVLARVPQETSKTRDITGGLPRVAE
                     LFEARSPKDAGLLAEITGTVSFGKDTKGKQRLVITDLEGVSHEYLIPKDKHV"
BASE COUNT          546 a         1153 c         1162 g          732 t
ORIGIN      
        1 acgtgcttgt ccttcgggat caggtactcg tgcgagacgc cctccagatc ggtgatgacc
       61 aggcgctgct tgcccttggt gtccttgccg aaagacaccg ttcccgtgat ctcggcgaga
      121 agccctgcat ccttgggcga gcgcgcttcg aagagctccg ccacgcgagg caatccaccg
      181 gtgatgtcgc gggtcttgga ggtctcctgc ggaacgcgcg cgagcacctc acccacctgc
      241 acctgctggc cgtcccgaac cgtgatgagc gatccgacct ggaacgtgat ggtgatcgga
      301 tggtcggtgc ccgcgatgcg aatctcctcg ctcgattcgt tcagcaattt cacctgcgga
      361 cgcaggccct tgcccgccgc ggcgcggcgc ttgggatcga tcacgacgag cgtcgagagg
      421 ccggtcacct cgtcgatctg cttggcgacg gtgacgcctt cctcgatgtg ctcgaagcgc
      481 acgatgccgc cgtgctcggt gatgatcggg cgggtatgcg ggtcccacga ggcgagcgtc
      541 tgccccgcct tgaccggctt gctgtcggca gcgaggagcg tcgcgccgta gggcaccttg
      601 tggcgctcgc gctcgcggcc gttgtcgtcg gtgacgagca cttcgccggt ccgggagatc
      661 accaccttct cgcccttcgg gttggtgacg tagcgtatct gcggggtaaa gcgcacggtg
      721 ccgttcgact tcgcttccac ctggttcgcc gcggcggcgc gtgaggccgc cccgccgata
      781 tggaaggtgc gcatggtgag ctgagtcccc ggctcgccga tcgattgcgc cgcgatcacg
      841 ccgatcgcct cgccgacgtt gacggaggaa ccgcgcgcaa ggtcgcgtcc atagcaggcg
      901 gcacacaagc cccagcgtgt gtcgcaggtg agcggcgtgc gcacccgcac ctcgtcgatt
      961 ccgagcgcct cgatattctc gacctcgtct tcgttcagga gcgtggcggc gttgtagagc
     1021 acttcctggg tttcagggtg cagcaaatcg gaggccgtga cccggccgag aatgcgctct
     1081 ctcaacgcct cgaccacttc gcccccttcg accagcgcct tcattgcaac gccgttcgtg
     1141 gtgcggcagt cctcttccgt gaccacgaga tcctgggtca cgtccaccag gcgccgcgtg
     1201 agatagcccg agttcgccgt cttcagggcg gtgtcggcga ggcccttgcg cgcgccgtgc
     1261 gtcgagatga agtactgcag cacattcagc ccttcgcgga agttggccgt gatcggcgtc
     1321 tcgatgatcg agccgtcggg cttggccatc aggccgcgca tgccggcaag ctggcggatc
     1381 tgggccgcgg agccgcgggc gccggaatct gccatcatgt agatggagtt gaacgattcc
     1441 tgcttgacct tctgcccctt gcggtcggtg acgtcctccg aggaaagctg gtccatcatc
     1501 gctttcccga cctggtcgcc ggcgcggccc cagatatcca cgaccttgtt gtaacgctcg
     1561 cccactgtga cgaggcccga ggtgtactgg gtctcgatct ccttcacctc gccttcggcg
     1621 gcggtgatga ggtcgtgctt ctgtgtcggg accagcatgt cgtcgacgca gatcgaaata
     1681 ccggcgcggg tggcgatcgt aaatccggcc tgcatgagct tatcggcgaa aatcaccgtc
     1741 tcgcgcagcc cgcagcggcg gaacgacgcg ttgatgagct tggagatctc ctttttcttg
     1801 agcgccttgt tgatcaattc aaagggtagg cccacgggaa gaatttccga aagcagggcg
     1861 cggccgaccg tggtccggtg gcggacgagc ttttcgattt tttcaccgcc ggggccgaat
     1921 tcggcttcgc ggatgcgcac cgtgaccccc gcgtgcagct cgaccttccc ggagtcgtac
     1981 gcgcgcgcta cttcggtcac gtcggcgaac accatggctt cgccccgggc gttgacgcgc
     2041 tcgcgcgtcg cgtagtaaag ccccagcacg atgtcctgcg agggcacgat gatcgggtca
     2101 ccgttcgcgg gcgaaaggat gttgttcgag gcgagcatca gtgtgcgcgc ttccatctga
     2161 gcttcgagcg acagcggcac gtgcaccgcc atctggtcgc cgtcgaagtc ggcgttgaac
     2221 gccgcgcaca ccagcgggtg cagctggatc gccttgccct cgatcagcac gggctcgaag
     2281 gcctggatgc cgaggcggtg cagggtcggc gcgcggttga gcatcaccgg gtgctcgcgg
     2341 atcacttcct cgaggatgtc ccacacgacg ggggtctcgg cctcgacttc gcgcttcgcg
     2401 gccttgatgg tcgtggcgat gccctgcatc tcgagcttgt tgaagatgaa gggcttgaag
     2461 agctccagcg ccatcttctt gggcaggccg cattggtgca gcttcaactg cgggcccacc
     2521 acgatcacgg agcggcccga gtagtccacg cgcttgccga gcaggttctg gcggaagcgc
     2581 ccgcccttgc ccttgatcat gtcggcgagg gatttcaagg ggcgcttgtt ggcgcctgtc
     2641 atcgccttgc cgcggcggcc gttgtcgagc agcgagtcca ccgcttcctg gagcattctc
     2701 ttctcgttcc tgacgatgat ctcgggcgcc ttaagctcga ggaggcgctt gaggcggtta
     2761 ttgcggttga tgacgcggcg atacaggtcg ttcaggtcgg aggtcgcgaa acggccgccg
     2821 tccagcggta ccagcggcct caactccggg ggcagcaccg gcagcacttc catgaccatc
     2881 cagtccggct tgatgccgga cttctggaaa ccttcgagca ctttgaggcg cttcgccagc
     2941 tttttgatct tggtgtcgga gccggtgctc tcgagctcct tcctcagggt gtcgatctgc
     3001 tcgttgaggt tgatcgcccg caggagctcg cgaatcccct cggctcccat gctcgccgag
     3061 aaatcgtcgc cgtgctcctc gatcttggcg aggtagtcct cctcggagag gagcttggcc
     3121 cgcttgatct tctcgtcgtt gaccatgccc gcgtcggtga cgacgtaggc ctcgaagtag
     3181 agcacgcgct cgatgtcccg gagggtcatg tcgagcacga ggccgaggcg cgagggcagg
     3241 ctcttcagga accagatgtg cgcgaccggg ctggcgagct cgatgtgccc catgcgctcg
     3301 cgacgcacct tggcgagcgt gacctcaacg ccgcacttct cgcagatcac cccgcggtgc
     3361 ttcaggcgct tgtactttcc gcacaggcac tcgtagtcct tgatcggccc gaagatcttg
     3421 gcgcagaaca gaccatcccg ctccggtttg aacgtgcggt agttgatcgt ctcgggcttc
     3481 ttgacctcgc cgtacgacca cgagcggatc ttttccggcg atgccagccc ggtcttgatc
     3541 gcgtcgaact cttcttcctg ggtgacctgc ttgaacagat cgagcaatgc ttt
//
LOCUS       DASPZD010000331         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1701535, whole genome shotgun sequence.
ACCESSION   DASPZD010000331 DASPZD010000000
VERSION     DASPZD010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701535"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VEM33_06445"
     CDS             <1..96
                     /locus_tag="VEM33_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYS51499.1"
                     /translation="FGGTKQSGLGREGAHEGLMEFMETQYISVSW"
     gene            305..1306
                     /locus_tag="VEM33_06450"
     CDS             305..1306
                     /locus_tag="VEM33_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HYS51500.1"
                     /translation="MAAEKLDVLMVGPVMPLIEQGISRAFTLHKLWEAPDRDALIAKL
                     APRLRAIVAGGGKHEPMGPEFLGRFPKLEIISSFGVGYDHVDAKWAAAHGIVVTNTPD
                     VLTEEVADTALGLLLCTVRELPQAERYLRAGKWLEGDYPLTRATLRDRTIGMVGLGRI
                     GRAIARRLDAFRVPVVYHSRHPQPGVPYRHYPNLVDMARDVDVLMVVTPGGPTTKHLI
                     DANVLEALGPDGILINMSRGSVVDEPALIAALEAGKILSAGLDVFANEPRVPPELMAM
                     ENVVLFPHVGSASVYTRDAMGQLVVHNLLSWAAGKGPLTPVPESQGVRGRKSEVSNQ"
     gene            1427..2122
                     /locus_tag="VEM33_06455"
     CDS             1427..2122
                     /locus_tag="VEM33_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HYS51501.1"
                     /translation="MTRPQFTATLLIASIGSAGFASLAHAQGDAGTPMVGAWELSNAD
                     RDKTCTINFKLDAAGAGRALEFQKGCAEAFPETRDIVAWVIDKDDTLRLMNAKGKAIF
                     ELTEVENGLFEPLRPSENLVFLQTQAAAQGRERTADAMFGDWALMRAGGKPMCQISLL
                     DEAHDTESFDMKVKPGCDSLVTNFAPKSWRMDRGLLLVLSERSDPWRFEEEESGTWHR
                     IPQGRQPLLLVRQ"
     gene            2182..2409
                     /locus_tag="VEM33_06460"
     CDS             2182..2409
                     /locus_tag="VEM33_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51502.1"
                     /translation="MTSAARDLLEEIESWPKEDQDELVEIAREIKARRTGTYVMTDEE
                     RAAVREGLEQARRGEFVSDEEMEAFWKRHGV"
     gene            complement(2803..3108)
                     /gene="clpS"
                     /locus_tag="VEM33_06465"
     CDS             complement(2803..3108)
                     /gene="clpS"
                     /locus_tag="VEM33_06465"
                     /inference="COORDINATES: protein motif:HMM:NF009564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HYS51503.1"
                     /translation="MSDITSKPRTKVKLKTERPRLHKVILINDDYTPREFVVAVLKAE
                     FRMNEDQAYRVMITAHKRGVCVVGVFAKDVAESKATRATEAGRSKGYPLLFTTEPEE"
     gene            complement(3201..>3650)
                     /gene="radC"
                     /locus_tag="VEM33_06470"
     CDS             complement(3201..>3650)
                     /gene="radC"
                     /locus_tag="VEM33_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYS51504.1"
                     /translation="EEAITDLKIVQAAANELLRGGLREKPVLSSWSAVLDYCRTAMAY
                     AEREELRVLYLDKRNQLIADERQQVGTVDHTPVYPREVVKRALELSASALILVHNHPS
                     GDPSPSRADVEMTQAIVEAARALGISLHDHIIVGRQGHASFKALGLI"
BASE COUNT          657 a         1150 c         1190 g          653 t
ORIGIN      
        1 ttcggcggca ccaagcagtc gggcctgggg cgcgaaggcg cgcacgaagg cctgatggag
       61 ttcatggaga cgcagtacat ctccgtgagc tggtgatctc ttttacctcc ccggagggga
      121 ggtcgatcgg gggtaagccc gatcgggtgg ggagtcggac cccaccccgg tgtctcgctg
      181 cgctcgacac cgaccctccc cttcggggga gggaaaagga acagcgcctc ccattgactt
      241 gcgcgcgatc ctgctgagag tccgcgggtc cttttgccga gacgctgtgc gacggaggaa
      301 cgggatggcc gccgagaagc tcgatgtcct gatggttggg ccggtgatgc cgctgatcga
      361 gcagggcatc tcccgggcgt tcacgctgca taaattgtgg gaggcgcccg atcgcgacgc
      421 cctgatcgca aaacttgcgc cgcggctgcg tgcgattgtc gccggcggcg gcaagcacga
      481 gccaatgggc cccgagtttc tcggccgctt cccgaaactc gagatcatct cgagcttcgg
      541 ggtgggctac gatcatgtcg atgccaaatg ggctgcggcc cacggcatcg tggtcaccaa
      601 cacgcccgac gtgctcaccg aggaggtcgc cgacacggcg ctcggcctgc tgctgtgcac
      661 ggtccgcgag ctgccgcagg ccgagcgcta tttgcgcgcc ggcaagtggc tcgaaggcga
      721 ctacccgctg accagggcga cgctgcgcga tcgcacaatc ggcatggtcg ggctcggccg
      781 catcggcagg gcgatcgcgc gccggctcga tgcgttccgc gttcccgtcg tctatcacag
      841 ccgccatccg cagccggggg tgccgtaccg gcattacccc aatctcgtcg acatggcgcg
      901 cgacgtcgac gtgctgatgg tggtcacgcc gggcggtccc acgaccaagc atctgatcga
      961 cgccaacgtg ctcgaggcgc tcgggcccga cggcatcctg atcaacatgt cgcgcggctc
     1021 ggtggtggac gagccggcgc tgatcgccgc gctcgaggcg ggcaagattt tgtcggccgg
     1081 cctcgacgtg ttcgcgaacg agccgcgggt gccgcccgag ctgatggcga tggaaaacgt
     1141 ggtgctgttt ccccacgtcg gctccgcctc ggtctacacc cgcgacgcca tgggccagct
     1201 cgtggtccac aatctcttgt cctgggccgc cggcaagggc ccgctcacgc cggttccgga
     1261 gtcccagggc gtcagaggtc ggaagtcaga agtcagtaat cagtagggag ggggccggcg
     1321 tacgagttca tattttcgcc acttctgact actgacttct gatttctgat ccctggaacg
     1381 gccacatccg accactgact tctgatcacc gaaccctgga acggctatga ctcgtccgca
     1441 attcaccgcc acgcttttga tcgcgtcgat cgggagcgcc ggtttcgcct ccctcgccca
     1501 cgcgcagggc gatgccggta cgccgatggt cggcgcctgg gagctctcca atgccgatcg
     1561 cgacaagacc tgcaccatca atttcaagct cgatgcggcc ggggccggac gcgcgctcga
     1621 attccagaag ggctgcgccg aggccttccc cgagacgcgg gacatcgtcg cctgggtgat
     1681 cgacaaggat gacacgctgc gtctgatgaa cgccaaaggc aaagcgattt tcgagctcac
     1741 cgaagtcgag aacgggctgt tcgaaccgct gcgtccgagc gaaaatctgg tgttcctgca
     1801 gacgcaggcc gccgcacagg gccgtgagcg caccgccgac gccatgttcg gcgactgggc
     1861 cttgatgcgc gccggcggca agccgatgtg ccagatcagc ctgctcgacg aagcccacga
     1921 caccgagagc ttcgacatga aggtgaagcc cggctgcgac agcctggtga ccaatttcgc
     1981 gccgaaatcc tggcggatgg atcgcggatt gctgctggtg ctgtccgagc gcagcgaccc
     2041 gtggcggttc gaggaggagg aaagcggcac ctggcaccgc attccgcaag gccgccagcc
     2101 gctgctgctg gtgcggcagt aacgggggcg ctcagctgtg ctatactggc gagcgcaccg
     2161 gcaatcgcct aagtgagcgt catgacttct gccgcgagag accttctcga agaaatcgag
     2221 tcctggccca aggaggatca ggacgagctt gtggagatcg cgcgtgaaat caaggcacgt
     2281 cgcacgggca cctatgtgat gaccgatgaa gaacgcgcgg ccgtgcgcga agggctcgag
     2341 caggcgcgcc gcggcgagtt cgtgtccgac gaggagatgg aggcgttctg gaaacgccac
     2401 ggcgtatgag agttcgcttc agcgggcgcg cgcaaagaga catcgaagaa atataccggt
     2461 atctggtcga caaaagccca ctcggagcac aaaacgtact ccgttccatc cgggaagcgg
     2521 ccgaattcat cggtagaaac cctcatgcgg cgccgaaaac ggacgatccc gagatccgaa
     2581 tgagagtggt tcggcgacat cgatacaagg ttttcttccg gatcggttcc gatactgtcg
     2641 aaatcattca cgtgcgccat ccgtcgcggc gccctctgga aatagaacgt tagtcgtagg
     2701 atgggttgag cgcagcgaaa ccccttgtgg gcggccggac aggcgctggc agtccagagt
     2761 atttttggag agccgacggg gttcgctgcc tgctcaaccc atctattcct ccggctcggt
     2821 cgtgaacagc agcggataac ccttgctgcg gcctgcttcc gtcgctcgcg ttgccttgct
     2881 ctcggcgaca tccttcgcga acacgccgac cacgcagacg ccgcgcttgt gcgcggtgat
     2941 catcacccgg tacgcctgat cctcgttcat gcgaaactcg gctttcagca ccgcgacgac
     3001 gaactcgcga ggcgtataat cgtcgttgat caggatgacc ttgtgcaggc gcggtcgttc
     3061 cgtcttcagc ttaactttgg tgcgtggctt gctggtgatg tcgctcatcg accgctgctc
     3121 ccgaccaggt gtagctgcgg aaacagattc aacgcttttg caggtccgga gttgccggag
     3181 taagcccggt ccctcacaaa tcaaatcagt cccagcgcct tgaagctggc atgaccctgg
     3241 cggccgacga tgatgtggtc gtgcagcgaa atacctaatg cgcgggcggc ctcgacgatc
     3301 gcttgagtca tctccacgtc ggcgcgcgag gggctcggat cgccggaggg atggttgtgc
     3361 accaggatca gcgcgctcgc cgagagctcg agcgcgcgct tgaccacctc gcgcggatag
     3421 accggcgtgt ggtcgacggt gccgacctgc tggcgctcgt cggcgatgag ctggttgcgc
     3481 ttgtcgagat agagcacgcg cagctcctcc ctctcggcat acgccatggc ggtgcggcaa
     3541 taatcgagca ccgccgacca cgacgagagc accggctttt cccgcaggcc gccgcgcaac
     3601 agctcgttcg cggccgcctg gacgatcttc aggtcggtga tcgcctcctc
//
LOCUS       DASPZD010000332         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_376115, whole genome shotgun sequence.
ACCESSION   DASPZD010000332 DASPZD010000000
VERSION     DASPZD010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376115"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..2556
                     /locus_tag="VEM33_06475"
     CDS             280..2556
                     /locus_tag="VEM33_06475"
                     /inference="COORDINATES: protein motif:HMM:NF018620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HYS51505.1"
                     /translation="MRNELDEKYGSADAARYQKAASTDPASVESWQKAKQVFDRHCVL
                     CHACSDAPCQLSLASFEGIARGTNKTPVYDGGRLRAADPTRLFLDAHGPAEWRKRGFS
                     PVLNERASTPGAELDASVLFRALRLKQDAGAKPLPAKTAADAPQSCPAIEEFDRFAAA
                     SPQAGMPYGLPALSAAESGAIADWLNAGAPYANPPGPSPAALKQAARWEAFFNAEDTK
                     ARLMSRYLFEHLFFAHIYFGETGAQWFFQMVRSKTPPGQPLELIVTRRPYDDPGVARV
                     YYRLQPVQETFVIKTHIPYRLDDARMEKFRGWFIDNLVTVTALPSYEPGAASNPFATF
                     REIPIRSRYRFMLEEAHFTISGFIKGTVCRGQIALGVINDRFWVFFLNPNLPTLERDA
                     EFLAGQADYLRLPAESGSNAFLLDWLGYSRSETKFLQAKAEYYVKILKFLDTAPTLDL
                     LWDGDGVNPSAGLTLFRHFDQGSVVQGLVGAPPKTAWIIGYPLLERIHYLLVAGFDIY
                     GNVGHQLNTRIYMDFLRMEGEAHFIALLPKASRKSVRDHWYRGAESRAKEFIDGAATH
                     FPLETGIAFKTAAPAQELMLMGRERLQPALARKAPLARDKAGPEVQAAIVRLGNLEGR
                     RVSFMPQLAWLIVDESGSERHFTVLHNNAHSNISRLFDEDKRRLPDEDTLTVLEGLVG
                     AYPNVFYRVKAGELPAFVEGVERLASAADYARLAARFGVSASDPEFWKVSDRVIDAFA
                     RQSPVDAALPDYGRFELR"
     gene            complement(2578..>3471)
                     /locus_tag="VEM33_06480"
     CDS             complement(2578..>3471)
                     /locus_tag="VEM33_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HYS51506.1"
                     /translation="KKTHMRIKLSAVSAALLASLVLAAPALGQGKDQKLDGTLGKIKT
                     SGVLNIGYRESSPPFSFMGPDKRPVGYSIELCMHVASAIQKQLGLADLKLGWVPVTAQ
                     NRIDMVVQGKVDLECSTTTASLSRQERVDFSLMTFVDGGGLLTTAGANLRGPADLAGK
                     RIAVIPGTTTEKALAEYLKTEFVTVQTVRVKDHAEGFAALEQDQADAFASDRGVLIGL
                     AVTSKDPKRFLLPDLTFSYEPYALMMRRNDAAFRLAVNRALAGLYRSGGVGPIYDRWI
                     GVFSKPSPPLRAMYLLNGLPE"
BASE COUNT          590 a         1153 c         1128 g          600 t
ORIGIN      
        1 acctccggcc gatcgagcag ttggcgcagc agaaaacccg ccgcaacgat caggacgacg
       61 atggcagcaa aggaaaaagc ggcgtagcga acagccttgc tccgccgccg ccgattggaa
      121 gtcttgccca cggtgggcgg attctatcgt gcgggcgggc gccacactcg ggcgtcaaat
      181 cccttgacgt aatcgcgtgc cgggcacaag ctcgcgctcg acatgagggc cctgattctc
      241 gctatctcag tcgtgctact cgccagttgc gcagcgatcg tgcgcaacga gttggacgag
      301 aagtacggct ccgccgatgc ggcgcgctac cagaaggccg caagcaccga cccggcgtcg
      361 gtggaatcct ggcagaaagc gaagcaggtg ttcgaccggc actgcgtcct gtgccacgcc
      421 tgctccgatg ccccttgcca gctgagcctg gcttcgttcg aaggcattgc gcgcggcacg
      481 aacaagacgc ctgtatacga cggcggacgg ctgcgcgccg ccgatccgac acgcctgttt
      541 ctcgatgcgc acggaccggc ggagtggcgc aagcgcggct tttctccggt tctgaacgag
      601 cgcgcctcga cccccggggc ggaactcgac gcgagcgtcc ttttccgcgc gctgcgcctg
      661 aagcaggacg cgggagcaaa gccgctcccg gcgaagaccg ccgccgatgc gccgcaatcc
      721 tgtccggcga tagaggaatt cgatcgtttc gccgccgcct cgccgcaggc ggggatgccc
      781 tatggcctgc cggcgctgtc ggccgcggag tcaggcgcta tcgccgactg gctcaacgcc
      841 ggcgcgcctt acgcgaaccc gcccgggccg tcgccggcgg cgctgaaaca ggcggcgcgc
      901 tgggaggcgt tcttcaacgc cgaagacacg aaagcgcggc tcatgagccg ctatctcttc
      961 gagcacctgt tcttcgcgca catttatttc ggcgagaccg gtgcgcagtg gtttttccag
     1021 atggtgcggt cgaagacccc gcccggacag ccgctcgagc tcatcgttac gcgcaggccc
     1081 tacgacgatc ccggcgtggc gcgcgtctac tatcgcctgc agccggtgca ggaaacgttc
     1141 gtcatcaaga cccatatccc ctaccggctg gacgacgcgc gcatggagaa attccggggc
     1201 tggttcatcg acaacctcgt gacggtcacg gcgctgcctt cctacgaacc gggcgccgcg
     1261 tccaacccgt tcgcgacctt ccgcgagatc ccgatccgct cgcgctaccg cttcatgctg
     1321 gaggaggcgc atttcaccat ctccggattc atcaagggga cggtgtgccg cggccagatc
     1381 gcgctcggcg tcatcaacga ccgcttctgg gtgttctttc tcaatccgaa cctgcccacg
     1441 ctcgaacgcg atgccgaatt tctcgccggg caggccgact acctgcgcct gcccgccgag
     1501 agcggcagca acgcttttct gctcgactgg ctcggctaca gccgctccga aacgaaattc
     1561 ctccaggcga aggccgagta ctacgtgaag atcctgaagt tcctggatac ggctccgacc
     1621 ctggacctgt tgtgggacgg agacggcgtc aaccccagcg ccgggctcac cttgttccgt
     1681 catttcgacc agggctcggt ggtgcagggg ctggtgggag cgccgccgaa gaccgcgtgg
     1741 atcatcggct atccgctcct cgagcgcatc cactatctgc tcgtggccgg tttcgacatc
     1801 tacggcaacg tcgggcatca gctcaacacg cgcatctaca tggacttcct gcgcatggaa
     1861 ggcgaggcgc atttcatcgc gctgctgccc aaggcgagcc gcaagtccgt gcgcgaccac
     1921 tggtatcgcg gcgccgaatc cagggcgaag gagttcatcg acggcgccgc gacgcatttt
     1981 ccgctggaga ccgggatcgc attcaagacc gccgcgccgg cgcaggagct gatgctgatg
     2041 gggcgcgagc ggctgcagcc ggcgctggcc cgcaaggcgc cgctcgcgcg cgacaaggca
     2101 ggcccggagg tgcaggccgc gatcgtgcgc ctcggcaatc tggaaggacg gcgcgtgtcg
     2161 ttcatgccgc agctcgcctg gctcatcgtc gatgaaagcg gcagcgagcg ccacttcacg
     2221 gtgctgcaca acaacgccca cagcaacatc tcgcggctct tcgacgagga caagcggcgc
     2281 ctgcccgacg aggacacgct gaccgtcctc gagggcctgg tcggcgcgta tccgaatgtc
     2341 ttttatcgcg tgaaggcggg cgaattgccg gcgttcgtcg agggcgtcga gcgcctcgcg
     2401 agcgcggccg actacgcgcg ccttgctgcg cgattcggcg tgtcggcgtc cgatccggaa
     2461 ttctggaagg tgagcgaccg cgtgatcgac gccttcgcga ggcagtcgcc ggtcgatgcg
     2521 gcgctgccgg actacgggcg cttcgagttg cgctgaggta ctcgcgtctt aatcgcgcta
     2581 ctcgggaagc ccgttgagga ggtacatggc ccgaagcggc ggactcggtt tgctgaagac
     2641 gccgatccag cgatcgtaga tgggcccgac gccacccgag cggtagagcc cggcgagcgc
     2701 ccggttcacg gccagacgga acgctgcgtc gttgcggcgc atcatgagcg catagggctc
     2761 ataggagaac gtgaggtccg gcaagaggaa gcgcttcggg tccttcgagg ttaccgcgag
     2821 gcctatcagc acgccgcggt cggaggcgaa ggcatcggcc tggtcctgct cgagggccgc
     2881 gaatccctcc gcatggtctt tgacgcggac tgtttgaacg gtaacgaact ccgtcttcag
     2941 gtattcggcc agggccttct cggtcgtggt gccggggatg actgcgatgc gcttgcccgc
     3001 gaggtcggcc ggcccgcgca gattggcgcc cgccgtggtc agcagacctc caccgtccac
     3061 gaaggtcatc aggctgaagt ccacgcgctc ctggcgcgac aggctcgccg tggtcgtgct
     3121 gcattcgaga tccactttgc cctgcaccac catgtcgatg cggttttggg ccgtcaccgg
     3181 cacccagccc agcttgagat ccgccagccc gagctgtttc tggatggcgc tcgcgacgtg
     3241 catgcacagc tcgatcgagt agcccaccgg ccgtttgtcc ggacccatga aagagaacgg
     3301 cggcgaagac tcgcggtagc cgatgttcag gacgcccgag gtcttgatct ttccgagcgt
     3361 tccgtccagt ttctgatcct taccctggcc cagggccggg gcggccagga ccagcgacgc
     3421 cagcaacgcc gcgctaactg cactcagctt gatacgcatg tgagtttttt t
//
LOCUS       DASPZD010000333         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_186, whole genome shotgun sequence.
ACCESSION   DASPZD010000333 DASPZD010000000
VERSION     DASPZD010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="VEM33_06485"
     CDS             <1..1733
                     /locus_tag="VEM33_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYS51507.1"
                     /translation="PYNQVDKGIVAEMDSAARQAASNQPAAVREALLPPLKAELAKIP
                     GTPAEPRFDLVVNAAPAQQVFMSIVSGTRYSMVVNPEVGGRLSLNLKDVTVREALEAI
                     REVYGYEFRVDGTRIYVEAAGMRTRVFHVSYLSSLRTGRSDVRVSSGSTSGTIGGQPM
                     GGGAGAAPVAAGAQAGQSVNTIGLGQLNQVTNDVTRVSTFSQSDFWQELTTALRTLVG
                     TGAGRSIVVSPQSGVVVVRAMPQEIRSVESYLKAIRVSVERQVMLEAKIIDVTLNDAF
                     QAGVNWAGFSGTRFSGGVQGPGTTLGTTGPLVTSSPIGLAASPANRTVVSTTASAATA
                     ASAAAAGAAGLGVFGLALQTRNFAALLQFLESQGTTQVLSSPRVATLNNQKAVLKVGT
                     DDFFITNVKSTPVVNAATGVPVGATLEPTLSSFFSGVSLDITPQIDEDGNINLHIHPL
                     VSRVQNGTLAFDFGTGIGITNIQTAKSTINETDTMVRVQNGNIVALGGLMQLEVENGR
                     SGIPGVQDVPVVGSLFGNRARNTVKKELVILIKPTIIKSEADWEQDITATGDRLRGMG
                     EGSARTPGSQ"
     gene            1730..2620
                     /locus_tag="VEM33_06490"
     CDS             1730..2620
                     /locus_tag="VEM33_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS51508.1"
                     /translation="MIYLSHFGLREPPFGITPDTSFFYACASSQEALNMLLVAVANGE
                     GFLKITGEVGTGKTLLCRKFLATLDDSWISAYVPNPNLEPKTLLLALAEEMRVKLDSG
                     LDQHHLLKALTLALLDFARQKRRVVVCLDETQAMPLESLETLRLLTNLETEKRKLVQV
                     ILFGQPELDERLAHESIRQLRQRITFQHHLGTLTREETEYYLAHRLAIAGYSGEGVFA
                     PGAVKRIYRASRGVPRLVNILANKAMMLAYGEGSRRVTAQHARAAAADTPASFTLSRA
                     WLWLALACVAFSSIGWMILR"
     gene            2617..3780
                     /locus_tag="VEM33_06495"
     CDS             2617..3780
                     /locus_tag="VEM33_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS51509.1"
                     /translation="MSLINKMLRDLDKRHAAQDGATAPAGALSRHMRPVSGRTFASDF
                     FWRTMAVTMLFAVGWVAWLVWQLTPPHPIVTDLAYESARARTASPADVASPRSDSAPA
                     REAPAPQPPPAAPIPAAGPAPAQATPPQRSDKVNVDMLRLATELTTPIPQRSSKASPS
                     RSGSRARVGAAKAAAAQPALAGDLGAAPGKIDRRSNTSSHSRAESEFRRAVNLVNQGR
                     IAEGMDGLRRTLEIDPRHEAARQTLVALLLETKHVGEAAASLREGLALNSGNTGFAML
                     LARIMVESNDIPAALIVLQRHAAPVDRNPDFHAFAAALYQRLDRHKEAIEQYQTALGL
                     VPSAGVWWLGLGISFQSVGRPKDALEAFTRAKSAGNLGPDLVAFVDQRLRQLQ"
     gene            3792..>4081
                     /locus_tag="VEM33_06500"
     CDS             3792..>4081
                     /locus_tag="VEM33_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYS51510.1"
                     /translation="MQDRYREVYDSFKWQVPARFNIGAACCNRHSSDRSRFALYWEDE
                     GGAALAYTFWDLQQQANRLSNALAGLGVVRGDRVGIILPQRPETAIAHIACY"
BASE COUNT          731 a         1357 c         1323 g          670 t
ORIGIN      
        1 ccccatacaa ccaggtcgac aaggggatcg tcgcagagat ggacagcgcg gcccggcagg
       61 ccgcatcgaa ccagcctgcg gcggtgcgcg aagcgctgct gccccccctc aaagcggagc
      121 tggcgaagat ccccgggacg ccggccgagc ctcgtttcga cctcgtggtc aacgccgcac
      181 ccgcgcagca ggtgttcatg tcgatcgttt ccggcactcg ctacagcatg gtggtgaacc
      241 cggaggtggg cggaaggctg tctctgaacc tgaaggacgt cacggtgcgc gaggcgctcg
      301 aggcgatcag ggaggtttac ggatacgaat tccgtgtcga cggcacgcgc atttacgtcg
      361 aggcggccgg aatgcggacg cgggtgttcc acgtcagcta cctctccagc ctacgcacgg
      421 gccgcagcga tgtgcgggtg agctcaggct cgacttccgg aaccatcggg ggccagccaa
      481 tgggaggcgg ggcgggagcc gcgccggttg cggcaggtgc ccaggccgga cagagcgtga
      541 acaccatcgg ccttggccag ctcaatcagg tcaccaatga cgtgacacgc gtctcgactt
      601 tctcgcaaag cgacttctgg caggagctca ccaccgcgct gcgcaccctg gtcggaaccg
      661 gcgcggggcg cagcatcgtc gtcagcccgc agtccggcgt agtggtggtt cgtgcgatgc
      721 cgcaggagat ccgcagtgtc gagagctacc tgaaggcaat ccgcgtttct gtcgagcgcc
      781 aggtgatgct cgaggcgaag atcatcgacg tgacgctcaa cgacgccttc caggcgggcg
      841 tgaactgggc gggattctcg ggcacgagat tttcaggcgg agtccagggc ccgggcacga
      901 ctcttggaac gacaggccct ctggtgactt cctcccccat cggacttgca gcgagtcccg
      961 ccaaccgcac ggtcgtatcc acgactgcgt ccgcggcgac tgcggcttcg gctgcggcgg
     1021 ccggggcggc ggggctgggc gttttcgggc tcgcgctgca gacgaggaat tttgcggctc
     1081 tgctccagtt cctcgaatcg cagggcacga cccaggtgct gtcgagccct cgcgtggcaa
     1141 ccctcaacaa tcaaaaagcg gtattgaaag tcgggaccga cgatttcttc ataaccaatg
     1201 tcaagagcac acccgtcgtg aacgccgcca ccggcgtccc ggtgggagcg acgctggagc
     1261 cgacgctcag ctcgtttttc tcgggagtgt cgctcgacat caccccgcag atcgacgaag
     1321 acggcaacat caatctgcat attcaccccc tggtgagcag ggtccagaac gggaccctgg
     1381 cgttcgattt cggaacggga atcggaatta cgaacataca gacagccaag agcacgatca
     1441 atgaaaccga caccatggtc cgcgtgcaga acggaaacat cgtcgccctc ggcggcctga
     1501 tgcagttgga agtggagaac ggccgctccg gaattcccgg agtccaggac gtaccggtgg
     1561 tcggcagttt gttcggtaac cgcgcacgaa acacggtgaa aaaggaactc gtaattctga
     1621 tcaagcctac gataatcaag agcgaagcgg actgggagca ggacatcacc gcgaccggcg
     1681 accggttgcg cggcatgggg gagggctccg ccaggacgcc ggggtcccag tgatctacct
     1741 ctcccatttc ggtctgcgcg aacccccgtt cgggatcact cccgacacga gctttttcta
     1801 cgcgtgcgcg agcagccagg aggcgctgaa catgctgctc gtcgccgtcg cgaacggcga
     1861 gggtttcctg aagatcaccg gcgaggtggg caccggcaag acgctgcttt gccgaaaatt
     1921 cctcgcgacg ctcgatgaca gctggatcag cgcgtacgtt cccaacccga acctcgagcc
     1981 caagacgctg ctgctcgcgc tcgcggagga aatgcgggtc aagctggaca gcggcctcga
     2041 tcagcatcat ttgctcaagg cgctgaccct cgcgctcctc gatttcgcgc gccagaaaag
     2101 gcgcgtcgtg gtctgcttgg acgagaccca ggcaatgccg ctcgaatcgc tcgagacgct
     2161 gcggctgctc accaacctcg agaccgaaaa gcgcaagctc gtccaggtga tcctgttcgg
     2221 ccagcccgag ctcgacgaga ggcttgcgca tgaatcgatc cggcagctgc gccagcgcat
     2281 caccttccag catcatcttg gaactctgac gcgcgaggag acggagtact acctcgcgca
     2341 ccgtctcgcc atcgccggct attcgggcga aggcgtgttt gcccccgggg cggtcaaacg
     2401 gatctatcgt gccagccgcg gggtcccgcg gctcgtcaat attctcgcca acaaggcgat
     2461 gatgctcgcc tacggcgaag gctcgcgaag agtgaccgcg caacacgcgc gtgccgcagc
     2521 ggccgataca ccggcctcgt tcaccctgtc gcgcgcctgg ctctggcttg cgctcgcctg
     2581 cgtggcgttt tcgagcatcg gctggatgat cctgcgatga gcctcatcaa caagatgctg
     2641 cgggatctcg acaagcgcca tgcggcccag gacggagcga cggcgcctgc gggcgccctg
     2701 tcgcggcaca tgcgtccggt gtccgggcgc acgttcgcct ctgatttctt ctggcgaacc
     2761 atggcggtga cgatgctctt tgccgtgggc tgggtcgcgt ggctcgtctg gcagctgaca
     2821 ccgccgcacc cgatagtcac ggacctcgca tacgagtccg cgcgcgcgag gaccgcgtcg
     2881 cccgccgatg tcgcttcgcc gcgttccgat tccgctcccg cgcgcgaagc gcccgccccg
     2941 cagccgcccc cggcagcgcc gattcccgca gccggtcctg cgcccgcgca agcgactccg
     3001 ccgcaacgct cggacaaagt aaacgtcgac atgctgcggc tggcgaccga gctgacgacg
     3061 ccgattccac agcgaagctc gaaggcatcg ccctcccgat ccggctcgag agctcgcgtt
     3121 ggcgcggcga aggccgccgc ggcgcaaccg gctctcgccg gcgacctcgg cgccgcgccg
     3181 ggcaagatcg accggcgctc gaatacctcc tcgcacagtc gggcggaaag cgaattccgc
     3241 cgcgcggtca acctcgtgaa ccagggccgg atcgccgaag ggatggacgg tttgcgccgc
     3301 accctggaaa tcgatccgcg acacgaagct gcgcgccaga ccctggtcgc gctcctgctc
     3361 gagaccaaac acgtgggcga ggccgccgct tccctccggg agggcctcgc gctcaacagc
     3421 gggaacaccg gtttcgccat gctgctcgcg cgcatcatgg tcgagagcaa cgatatcccc
     3481 gcggcgctca tcgttctgca aaggcacgcc gcgccggtgg accgtaaccc ggactttcac
     3541 gcgtttgccg cggcgctcta ccagcggctc gatcgtcaca aggaggcgat cgagcagtat
     3601 cagacggcgt tgggcctggt gccgtcggcg ggcgtttggt ggctgggcct ggggatctcg
     3661 ttccagtccg tcgggcggcc caaggatgcg ctcgaggcct tcacgcgcgc caaatcggcc
     3721 gggaacctgg gccccgatct cgtcgccttc gtcgatcagc ggctcaggca actccagtag
     3781 ttgcgtccgg aatgcaggac cgctatcgcg aggtctacga tagtttcaaa tggcaggtgc
     3841 cggctcgatt caacatcggc gcggcgtgct gcaaccggca ctcatccgac cgctcccgct
     3901 tcgccctgta ttgggaggat gagggcgggg cggccctcgc atacaccttc tgggatctgc
     3961 agcagcaggc gaaccgcctg tcgaatgcgc tcgccggcct cggagtggtg cgcggcgatc
     4021 gggtcgggat cattctgccg cagcgcccgg aaacggcgat cgcgcacatc gcctgctacc
     4081 a
//
LOCUS       DASPZD010000334         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_386741, whole genome shotgun sequence.
ACCESSION   DASPZD010000334 DASPZD010000000
VERSION     DASPZD010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386741"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VEM33_06505"
     CDS             complement(<1..381)
                     /locus_tag="VEM33_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYS51511.1"
                     /translation="MPRKTSKTHSADATDWPAAIHSALKSQRVRQVSYVPDAGHKRLI
                     ELCHADKGMTAVPLTTEEEGVALAAGAWLGGERAALLMQSSGVGNCANALTLAATCGF
                     PLLKLVTMRGETGERNPWQVPMGQA"
     gene            479..1153
                     /locus_tag="VEM33_06510"
     CDS             479..1153
                     /locus_tag="VEM33_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYS51512.1"
                     /translation="MKLYYHPVSTTSRPVWLFIAESDIKCELRVVDLMKGEHYQPEYV
                     AINPNRMVPVLEDGDFRLTESSAILKYLAEKTGHTAYPKELHSRAKVNEMMDWINTQV
                     CREFAYGLVYPQIFPNHKRRSDEAHAGTIAWGKEKARAWLKILDENLLGTKKKYLCGE
                     QITIADYFGGPFVALGEIVRCNYSAYPNVNRWLDRMKTLKNWGKVHEVFRGFAASLKD
                     KAFEAV"
     gene            1504..>2226
                     /locus_tag="VEM33_06515"
     CDS             1504..>2226
                     /locus_tag="VEM33_06515"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HYS51513.1"
                     /translation="MATYAIGDVQGSFEELRALLGAFGFDRAKDRLWFVGDLVNRGPA
                     SLATLRFVRDLGDGAVTVLGNHDLHLLALAQGSVKAREDDTLGDVIAAPDRDGLLDWL
                     RHRPMVHVAAGYALVHAGLLPQWDVATAKALAGEVEVELRGPRHKEFLAQLYGSLPDR
                     WNDELRGIDRLRVIVNAMTRLRFCTPEGVMEFRTKGETAEAPPGYLPWFEVPGRKSAD
                     HTLVCGHWSALGLRMAPKLLALD"
BASE COUNT          392 a          683 c          722 g          429 t
ORIGIN      
        1 cgcctgaccc atgggaacct gccagggatt gcgctcgccc gtctcgccgc gcatggtgac
       61 cagcttcagc agcggaaagc cgcaagtcgc ggcgagggtg agcgcgttcg cgcaattgcc
      121 caccccgctc gactgcatga gaagcgccgc gcgctctcct ccgagccacg cgcccgccgc
      181 gagagcgacg ccctcctcct cggtcgtgag cggcacggcc gtcatgccct tgtctgcgtg
      241 gcagagctcg atcaggcgct tgtgacctgc atcggggaca taagacacct gccgcacgcg
      301 ctgcgacttg agcgcgctgt ggatcgccgc gggccagtcg gtggcgtctg cagagtgcgt
      361 tttcgatgtt ttccgcggca ttgttcgcct gatccagggg atgctacccg catcctgcgg
      421 cttaggtatg attacgggct tccccgcaac gccccaccgt ttgcaaggag aaaccggcat
      481 gaaactctac tatcaccccg tatccacgac cagccgcccc gtgtggctgt tcatcgccga
      541 gagcgacatc aagtgcgaat tgcgcgtggt cgacctcatg aagggcgagc actaccagcc
      601 ggagtatgtc gcgatcaacc cgaaccgcat ggtacccgtg ctggaggacg gggatttccg
      661 cttgaccgag agttcggcga ttctcaagta cctcgcggag aaaacgggcc atactgccta
      721 tccgaaagag ctgcactcac gggcgaaggt gaacgagatg atggactgga tcaatacgca
      781 ggtctgtcgc gaattcgcct acgggctggt ctacccgcag atcttcccga accacaagag
      841 gcgcagcgac gaggcgcacg cggggacgat cgcctggggc aaggaaaagg cccgggcctg
      901 gctgaaaatc ctcgatgaaa acctgctcgg gacaaagaag aagtatctat gcggcgagca
      961 gatcaccatc gcagactatt ttggcggtcc gttcgtcgcc ttgggcgaga tcgtgcgctg
     1021 caactactcg gcgtacccga acgtcaaccg ctggctggac cggatgaaaa cactgaaaaa
     1081 ctggggcaag gtgcacgagg tcttccgcgg attcgccgcc tcgctcaagg acaaggcctt
     1141 cgaggccgtt tgaggcgttg gctaccagcg ctggcggtgc cggtaccagg gttgattctt
     1201 gggttttttc ttgtccggct tcgcttccgg ggtttccggg gcgccctctt gatcgggagg
     1261 cggggctacg gaaggcggcg gcgacgcgcg cttccgggcg ctctccttcg atgcggaggg
     1321 cttcttcgga gctgacccgg agggtttttc cggagcccga tgcaacatct ttttcagact
     1381 gccaaggatc gatttggaaa gcttctcagc catgaccgat tcaacaagaa gttgagcggc
     1441 gccattctat tacatcggtt cgaggggcgg tcttgacggc tccttcgcgg agcgctccct
     1501 gccatggcta cctacgcgat cggcgatgtt cagggtagtt tcgaggagct gcgggcgctg
     1561 cttggcgcat tcgggttcga tcgtgcgaaa gatcgtttgt ggttcgtggg cgatctcgtg
     1621 aaccggggcc cggcctcgct cgccacactg cggttcgtgc gtgatctggg tgatggcgcg
     1681 gtgaccgtgc tcggcaacca tgatctgcat ctgctggcgc tcgctcaggg ctccgtcaag
     1741 gctcgcgaag acgatactct tggagacgtg atcgccgcgc ccgaccgcga cggcttgctc
     1801 gactggctgc gacaccggcc gatggtgcac gtggcggcag gctacgctct ggtccacgcg
     1861 gggctcctgc cgcaatggga cgtcgccacg gcgaaagcac tcgccggaga agtcgaggtg
     1921 gaactgcgcg gaccccggca caaggaattt ctcgcgcaac tttacggctc gctcccggat
     1981 cgctggaacg acgagctgcg cgggatcgac cggctgcggg ttatcgtcaa cgcgatgacc
     2041 cgtctgcgtt tttgcactcc tgaaggcgta atggagttcc gcacgaaagg cgagacggcc
     2101 gaggctcccc ccggctacct tccgtggttc gaggtgccgg gcaggaaaag cgctgatcac
     2161 accttggtgt gcggccactg gtcggcgctt ggcttgcgca tggcgcccaa gctcctcgcc
     2221 ctcgat
//
LOCUS       DASPZD010000335         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1246118, whole genome shotgun sequence.
ACCESSION   DASPZD010000335 DASPZD010000000
VERSION     DASPZD010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246118"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VEM33_06520"
     CDS             complement(<1..476)
                     /locus_tag="VEM33_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS51514.1"
                     /translation="MSEVLACRGLRKTFREGREAVHVLQGVSLKVGEREIVAIVGASG
                     SGKSTLLHLLGGLDDPTSGEVDVLGRPTHSLSDAERGQVRNRSLGFVYQFHHLLMEFT
                     ALENVAMPLLIRRTQPAEAREAAAAMLKEVGLAERLDHTPAELSGGERQRAALARAL"
     gene            complement(469..1710)
                     /locus_tag="VEM33_06525"
     CDS             complement(469..1710)
                     /locus_tag="VEM33_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HYS51515.1"
                     /translation="MQYELLVGLRYTRAERRNHFISFISLTSMLGIALGVAALIVVLS
                     VMNGFQKEVRARILSVVSHVQITGVDNRLADWQTVVRESAEHPQVAAAAPFVNAQGML
                     VFGAGVRGAVVRGIVPELEQKVAEIGLHMMAGKLESLVPGEFGIVLGSELARALGAKA
                     QDKVTLIAPQGLVTPAGILPRLKQFTVVGIFEVGMFEYDSGLALIHLEDAQKLYGMGD
                     RASGVRLKLHDLFQSREVTRDLMARLRGDLYISDWTRSHANYFRAVQIEKTMMFIILL
                     LIVAVAAFNIVSTLVMAVTDKQPDIAILRTLGASPGGIMKIFIVQGALIGAIGTLIGV
                     AGGIALALNIDVVVPFLERLLNVQFLSREVYYITDLPSDLQMSDVVSIALVSLALSLL
                     ATLYPSWRAARVNPAEALRYE"
     gene            1751..2740
                     /locus_tag="VEM33_06530"
     CDS             1751..2740
                     /locus_tag="VEM33_06530"
                     /inference="COORDINATES: protein motif:HMM:NF021646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HYS51516.1"
                     /translation="MHCELYIPDFFLRDGLQRAESAAAAETLIARGRRRRTALVSREA
                     WLFGRFGVPRQRDWPVAPYTLLADGGEPGPHYWMRADPVHLQVGRDSLGLADNTAFEV
                     SRAESEALADALNRHFGQTMLVYPLRPARWYVRLEKAPDMQTTPAAAARGAAIDEKLP
                     AGPDAMRFNALMNEAQMLLHEHPVNAEREARGEPALNSIWFWGGGVIDAERARPFSTV
                     FSDDPLARGLALAAGIPALALPKDAGSALAAVGDEGRVLAVADAPREAQLRGRSAALE
                     RDWFLPLLAALKSGRIGMLTLHLCSADSLLEVETVRSDLRHFWRLRRPLSAYA"
BASE COUNT          476 a          971 c          937 g          456 t
ORIGIN      
        1 agcgcgcgag cgagcgccgc acgctggcgc tccccccctg aaagctccgc gggggtgtga
       61 tcgaggcgct cggcgaggcc gacttccttc agcatcgctg cggcggcttc gcgcgcttcg
      121 gcgggctgcg tgcgccggat cagaagcggc atcgccacgt tctcgagcgc ggtgaactcc
      181 atcagcaggt gatggaactg gtagacgaat cctagcgaac ggttgcgtac ctgaccccgt
      241 tccgcatcgg aaagactgtg cgtgggccgg ccgagcacgt cgacctctcc cgaagtcggg
      301 tcgtcgaggc ccccgagcag gtgtaagagc gtgctcttgc cggagccgga ggcgccgacg
      361 atggcgacta tttcccgctc tccgactttc agcgatacgc cttgaagcac gtgcacggct
      421 tcccttccct cgcggaaagt cttgcgcagg ccgcggcagg cgagcacctc actcatagcg
      481 cagcgcctcg gccggattca cgcgggcggc gcgccagctc ggatagagcg tcgcgagaag
      541 cgagagcgcg agcgacacca aggcaatgga aaccacgtcg ctcatctgga gatcggaagg
      601 caggtcggtg atgtagtaca cctcacgcga caggaactgg acgttcagga ggcgctcgag
      661 gaacggcacc accacgtcga tgttgagggc gagcgcgatg ccgccggcca cgccgatcag
      721 cgtgccgatc gcgccgatca gcgcgccctg cacgatgaag atcttcatga tcccgccggg
      781 gctcgcgccc agcgttcgca gtatggcgat gtcgggttgc ttgtcggtga ccgccatcac
      841 cagcgtcgat acgatgttga acgccgcgac cgcgacgatg aggagcagga tgatgaacat
      901 catggtcttc tcgatctgca ccgcgcggaa gtagttggcg tggctgcgag tccagtccga
      961 gatgtacagg tcgccgcgca ggcgcgccat caggtcgcgc gtgacttccc gcgactggaa
     1021 cagatcgtgc agcttgagcc gaacgcccga agcgcgatcc cccatgccgt agagcttctg
     1081 cgcgtcctcg agatgaatga gggcgaggcc cgagtcgtat tcgaacatgc ccacttcgaa
     1141 gatgcccacc acggtgaatt gcttgagccg cggaagaatg cccgccgggg tgaccagccc
     1201 ctgcggggcg atcagcgtca ccttgtcttg ggccttcgcg ccgagagcgc gcgcgagctc
     1261 ggacccgagc acgattccga attcacccgg cacgagcgac tcgagcttcc ccgccatcat
     1321 atgcaggccg atttcggcga ctttctgctc cagctccggc acgatgccgc gcaccaccgc
     1381 cccccggacg ccggcaccga acacgagcat tccttgcgcg ttcacgaaag gcgccgccgc
     1441 ggccacctgc ggatgctcgg ccgattcccg caccaccgtc tgccaatccg cgagccggtt
     1501 gtccaccccg gtgatctgca cgtgcgagac cacgctcaag atgcgcgcgc gaacctcttt
     1561 ctggaacccg ttcatcaccg agagcaccac gatcagcgcg gcgacgccga gcgcgatgcc
     1621 gagcatcgaa gtgagcgaga tgaaggagat gaagtggttg cggcgctcgg cgcgcgtgta
     1681 gcgtagcccg acgaggagtt cgtattgcac gcgcgaagtg tgccataacc ggtgataaac
     1741 tcgaaatcga atgcactgtg aactgtatat tcccgatttc ttcctccgcg acggacttca
     1801 acgcgcagag agcgcggcgg ccgcggagac gctgatcgcg agaggacgcc gcaggcgcac
     1861 agcgctcgtt tcgcgcgaag cctggctgtt cgggcgtttc ggcgtgccgc ggcagcgcga
     1921 ctggcccgtc gccccctaca ccctgctcgc cgacggcggg gagcccggac cgcactactg
     1981 gatgcgcgcc gatcccgtgc atctgcaggt gggccgcgac agcctcggcc tcgccgacaa
     2041 caccgccttc gaggtttcgc gcgccgagtc cgaagcactc gccgatgcgc tcaaccgtca
     2101 cttcggccag acgatgctcg tctacccctt gcggccggcg cgctggtacg tgcggctcga
     2161 gaaagcgccg gacatgcaaa ccacgccggc cgccgcggcg cgcggcgccg cgatcgacga
     2221 gaagcttcca gccgggcccg atgcgatgcg ctttaacgcg ctcatgaacg aggctcagat
     2281 gctgctgcac gagcaccccg tcaacgccga gcgcgaggcg cgcggggagc cggcgttgaa
     2341 cagcatctgg ttctggggcg gaggcgttat cgacgccgag cgcgctcgac ctttctcgac
     2401 ggttttcagc gacgatccgc ttgcgcgcgg cctcgcgctc gctgcgggaa tccccgcgct
     2461 cgcgttgccg aaggacgccg gctccgcgct cgccgccgtg ggggacgaag gccgcgtgct
     2521 tgccgttgcg gacgccccgc gggaggcgca gcttcggggg cgcagcgcag cgctcgagcg
     2581 cgactggttc cttccgctgc tcgccgcgct gaagtccggc cggatcggaa tgctcaccct
     2641 gcatctttgc agcgcggatt cgctgctcga ggtcgagacc gtacgctcgg acctgcgcca
     2701 tttctggcgg ctgcgcaggc cgctttcggc ctatgcatag ctgatgtccc agatcatcac
     2761 ccgcccggtt cccctgcgcg tgcacgaccg gctcgtgcgc gaaggcctgc acccggtact
     2821 ggcgcgcgtc tgcgcggcgc
//
LOCUS       DASPZD010000336         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1099271, whole genome shotgun sequence.
ACCESSION   DASPZD010000336 DASPZD010000000
VERSION     DASPZD010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099271"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..568
                     /gene="folK"
                     /locus_tag="VEM33_06535"
     CDS             92..568
                     /gene="folK"
                     /locus_tag="VEM33_06535"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYS51517.1"
                     /translation="MKAFIGLGANLGDPEAQVHRAFAALAELPGTRLLAASSLYRSAP
                     VGVVAQLDFVNAVAEVETALTARALLEALLAEERRFGRTRGFPNAPRTLDLDLLLYGD
                     RVIAEPGLVVPHPRMHERAFVLAPLAEIAPEIAIPGMGRAAALLAACTDQRVEKIG"
     gene            572..919
                     /locus_tag="VEM33_06540"
     CDS             572..919
                     /locus_tag="VEM33_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HYS51518.1"
                     /translation="MNSTVLAQTVGLLALSNVFMTFAWYAHLKELSSKPWLIAALASW
                     GVALFEYLLQVPANRIGFSQFSLAQLKIIQEAIALLVFVPFALFYMNQPLKLDYLWAS
                     LCIMGAVYFIFRG"
     gene            939..1928
                     /locus_tag="VEM33_06545"
     CDS             939..1928
                     /locus_tag="VEM33_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HYS51519.1"
                     /translation="MKLPALLVFLLLLPGPASGAKPGALPGLFTETFLDFREDVAQAA
                     RGGRRLMILFEQDGCPYCKELILTNFSQKTIADKARKNFLAVSLNIWGDREVIWMDGK
                     KFSEKGLAAFLKIQFTPTLVFFDERGAIVARLNGYYPPHRFEAVLDWVARRRERKIAL
                     AEYLRTAVREPAGEQLHDEPFFLKPPYDLRRRGAKPLAVFFETPSCAACDELHREGFR
                     RAAIAESLKRFDVARFALGERTPVTTPAGDRLPAEDWARSLGVSYTPSVVFFGADGRE
                     AFRIEAYLRPFHLWGSLEYVASGAYLREPNFQRFLQARADELRGRGEAVDLWR"
     gene            1996..2637
                     /locus_tag="VEM33_06550"
     CDS             1996..2637
                     /locus_tag="VEM33_06550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HYS51520.1"
                     /translation="MPLSKYHYVVVEGPIGVGKTSLARRLAGRLNSTLVLERPEQNPF
                     LPRFYEDMSRFALPTQLFFLFQRIGQLRELAQMDLFTRLTVCDFLLEKDPLFARLTLS
                     GEELHLYQQIYDSLKPQAPKPDLVIYLQAQPEILYERVRRRGVEFERPITGEYLALLA
                     DSYNRFFYHFDGSPVLIVNSEKLNFVSQDRDLELLIERVEAMRGNREFFNWGD"
     gene            2639..3433
                     /gene="panB"
                     /locus_tag="VEM33_06555"
     CDS             2639..3433
                     /gene="panB"
                     /locus_tag="VEM33_06555"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYS51521.1"
                     /translation="MTRMTIKALQKLRDDGVKIAVLTCYDASFAAMLDAAGLDSLLIG
                     DSLGMVLQGHDTTLPVTLADMTYHTRCVARGSKRSFLAADMPFGSYQESPQQAFRSAA
                     ELMAAGAQMVKLEGGAEFADTIRFLTQRGVPVCGHLGLTPQSVHQFGGYHVQGKGEDA
                     EQKLIEDAVELEAAGAGLIVLEAIPAKLGEQVTQRLRIPTIGIGAGPGCSGQVLVLHD
                     MLDVFPGKKARFVRNFMKGATGIADAAERYVRAVRDGSFPAEEHSF"
     gene            3442..4269
                     /gene="panC"
                     /locus_tag="VEM33_06560"
     CDS             3442..4269
                     /gene="panC"
                     /locus_tag="VEM33_06560"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYS51522.1"
                     /translation="MDIVTSVRDLRERLQREPDNVFVPTMGNLHAGHIQLMRLAKHRA
                     ACTVVSIFVNRIQFGPKEDFERYPRTLHADAEMMKEVGVDVVFAPTEVEIYPEPQTFV
                     VEPSDLQHILEGAFRPGHFRGVATVVLKLFNMVSPHTAIFGKKDYQQYVVLRDMVKQL
                     ALPVEIIPAETVRAEDGLALSSRNAYLTPAERTEAARLYRILQEVRAALLAGRRDYLK
                     LEAEALAKFNEKWAADYVAIRRQSDLQPPSRSDRNLVVLGAAKLGRPRLIDNVEVSL"
     gene            complement(4280..4516)
                     /locus_tag="VEM33_06565"
     CDS             complement(4280..4516)
                     /locus_tag="VEM33_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51523.1"
                     /translation="MFAIVYKSDGVPICVQMPGVSPDPVVVWNSESAARAFIESKGGG
                     KEFQPVSVNEQAMEKMAKALGCPIEKLMLEPYPS"
BASE COUNT          793 a         1607 c         1477 g          811 t
ORIGIN      
        1 gcgcctccgg accccgcgcc cgcttgaatc ggcgaattcc ccttcgtccg gccaaaaccc
       61 gatgacatcc gacgccccgc ggacggcgcc catgaaagcc ttcataggac tgggagcgaa
      121 tctcggcgac cccgaagccc aggtgcaccg cgcctttgcg gcgctcgccg agctgcccgg
      181 gacgcggctc ctcgccgcgt cttcgctcta ccgcagcgcg ccggtggggg tggtcgcgca
      241 gctcgatttc gtcaacgccg tcgccgaggt cgagaccgcg ctcaccgcgc gggccctgct
      301 ggaagctctg ctcgccgagg agaggcgctt cggccgcacg cgcggatttc ccaacgcgcc
      361 gcgcacgctc gacctcgatc tgctgctcta cggggatcgc gtgatcgccg agccggggct
      421 cgtcgttccc cacccgcgca tgcacgagcg tgccttcgtg ctcgcgcccc tcgccgagat
      481 cgcgcccgag atcgcgatcc cgggcatggg ccgcgccgca gcgctcctcg cggcgtgcac
      541 cgatcagaga gtagagaaga ttggataacc gatgaactcc accgtccttg cacagaccgt
      601 cggactgctc gcgctctcga atgtattcat gaccttcgct tggtacgcgc atctgaagga
      661 gctctcctcc aagccctggc tcatcgcggc gctcgcgtcc tggggcgtgg cgctgttcga
      721 gtacctgctc caggttcccg cgaatcgcat cggcttcagc caattcagcc tcgcgcaact
      781 gaagatcatc caggaagcga tcgcgctcct cgtgttcgtc ccgttcgcgc tcttctacat
      841 gaaccagcct ctcaagctcg actacctctg ggcgtcgttg tgtatcatgg gcgcggtgta
      901 tttcatcttc cgcggctgac ccgccgcggc acgcaacgat gaagctgccg gcgctgctcg
      961 ttttcctcct tctccttccg ggtccagcat cgggcgccaa gccgggcgcc ctgccgggct
     1021 tgttcaccga gactttcctc gatttccggg aagacgtggc gcaggccgct cgcgggggcc
     1081 ggcggctgat gattctcttc gagcaggacg gctgccccta ctgcaaggag ctgatcctga
     1141 ccaacttcag ccagaagacg atcgccgaca aggcgcgaaa gaatttcctc gcggtttcgc
     1201 tcaacatctg gggcgaccgc gaggtgatct ggatggacgg aaagaaattc tcggagaagg
     1261 ggctcgcggc tttcctgaaa atccagttca cgccgacgct cgtctttttc gacgagcgcg
     1321 gcgcgatcgt ggcgcggctg aacggctact atccgccgca ccgcttcgag gccgtgctcg
     1381 actgggtcgc ccgccgcagg gaaaggaaaa tcgcgctcgc cgagtacctg cgcaccgcgg
     1441 tccgcgagcc tgcgggggag cagctgcacg acgaaccgtt cttcctcaag ccgccctacg
     1501 atctgcgaag gcgcggcgcg aagccgctcg ccgtcttctt cgagacgccg tcctgcgctg
     1561 cctgcgacga gctgcaccgc gagggctttc gccgcgcggc aatcgccgag tcgctgaagc
     1621 gcttcgacgt ggcgcgcttc gcgctcgggg agcgcactcc ggtgacgact ccggcgggag
     1681 acaggctccc ggccgaggac tgggcgcgtt cgctcggcgt ttcctacacg ccttcggtgg
     1741 ttttcttcgg cgcggacggc agggaggcgt tccgcatcga agcctacctg cgcccgtttc
     1801 acctctgggg ctcgctcgaa tacgtcgcga gcggggccta tctgcgcgaa ccgaacttcc
     1861 agcgcttcct ccaggcgcgc gccgacgagc tgcgcgggcg cggcgaagcc gtcgatctgt
     1921 ggcggtagcg ctgcgcgcga gccctctctc gcggcgcgtg tatgatgggc gcccctttct
     1981 gcgagcccca gcctcgtgcc gctctcgaag taccactacg tcgtcgtcga aggaccgatc
     2041 ggcgtcggca agacgagcct cgccaggcgc ctggccggcc ggctgaactc gaccctggtg
     2101 ctggagcgcc cggagcagaa cccgttcctg ccgcgcttct acgaggacat gtcccgcttc
     2161 gcgctgccga cgcagctgtt cttccttttc cagcggatag ggcagctgcg cgagctcgcg
     2221 cagatggatc tcttcacccg gctcaccgtc tgcgactttc tcctcgagaa ggaccccctg
     2281 ttcgcgaggc tgacgctctc aggtgaggag ctgcacctgt atcagcagat atacgactcg
     2341 ctcaagccac aagcgccgaa acccgatctc gtcatctacc tccaggcgca gccggagatc
     2401 ttgtacgagc gggtgcgccg gcgcggggta gaattcgagc ggcccatcac cggcgagtat
     2461 ctcgccctgc tcgcggacag ctacaaccgt tttttctatc atttcgacgg gagcccagtg
     2521 ctgatcgtga attcggaaaa gctaaacttc gtgagccagg atcgcgacct cgagctgctc
     2581 atcgagcgcg tcgaagcgat gcgcggcaac cgcgagttct tcaactgggg agactaggat
     2641 gacacgaatg accatcaagg cgctgcagaa gctgcgcgac gatggcgtga agatagccgt
     2701 gctcacctgt tacgacgcga gcttcgcggc gatgctggac gcagccggcc tagacagcct
     2761 cctgatcggc gactcgctgg gcatggtgct gcagggacac gacacgacgc tgccggtgac
     2821 gctcgccgac atgacctacc acacgcgctg cgtcgcgcgc ggcagcaaac gcagtttcct
     2881 cgccgccgac atgcccttcg gcagctacca ggaatcgccg cagcaggcgt tccgcagcgc
     2941 ggccgagctg atggccgcgg gcgcgcagat ggtcaagctg gaaggcggcg cggagttcgc
     3001 cgacacgata cgcttcctca cccagcgcgg cgtcccggtg tgcggccacc tcgggctcac
     3061 gccgcaatcg gtgcaccagt tcggcggcta ccacgtgcaa ggcaaaggcg aagacgccga
     3121 gcagaagctg atcgaggacg ccgtggagct cgaggcggcc ggcgcgggcc tcatcgtgct
     3181 cgaggcgata ccggccaagc tcggggagca ggtcacgcag cgccttcgca tcccgaccat
     3241 cggcatcggc gccggcccgg gctgctccgg ccaggtgctc gtcctgcacg acatgctcga
     3301 cgtctttccc ggaaagaagg cgcgcttcgt caggaatttc atgaagggag ctaccggcat
     3361 cgcggacgcg gccgagcgct acgtgcgcgc cgtccgagac ggcagcttcc ccgccgaaga
     3421 gcacagtttc taggacgagc ggtggacatc gtcacttcgg tgagggatct gcgcgagcgg
     3481 ctgcagcgcg agcccgacaa cgtcttcgtc ccgaccatgg gcaatctcca cgccggccac
     3541 atccagctga tgcgcctcgc gaagcatcgc gcggcgtgca ccgtcgtcag catattcgtc
     3601 aaccgcatcc agttcgggcc gaaggaggat ttcgagcgct acccgcgcac cctgcatgcc
     3661 gatgcggaaa tgatgaaaga ggtgggcgtg gacgtcgtgt tcgcgccgac cgaggtggag
     3721 atctacccgg agccgcaaac cttcgtggtc gagccttccg acctgcagca catcctcgaa
     3781 ggcgcgttcc ggcccgggca cttccgcggc gttgccaccg tggtgctcaa gctgttcaac
     3841 atggtctccc cgcacaccgc gattttcggc aagaaggact accagcagta cgtggtgctg
     3901 cgcgacatgg tgaagcagct cgctctcccc gtggagatca ttccggcgga aaccgtgcgc
     3961 gccgaggacg ggctcgcgct cagctcgagg aatgcctacc tgacgccggc agagcggacc
     4021 gaagccgcgc ggctgtaccg gatcctgcag gaagtccgcg ccgcgctgct cgccgggcgg
     4081 cgcgactact tgaagctcga agcggaggca ttggcgaagt tcaatgagaa gtgggcggcg
     4141 gactacgtcg cgatcaggag acaatccgac ctgcaaccgc cgtcacgctc cgaccgcaac
     4201 ctcgtcgtcc tcggcgccgc gaagctcggg aggccccgac tgatcgacaa cgtcgaggta
     4261 tcgctctagg acggattatc taggacggat aaggctccaa catgagtttc tcgatggggc
     4321 aaccgagtgc cttggccatt ttttccatcg cctgctcgtt caccgacacc ggctggaact
     4381 ccttgccgcc gcccttgctc tcgatgaacg ccctggccgc gctctccgag ttccagacta
     4441 caaccgggtc gggcgagacc ccgggcatct gaacgcagat cggaacgccg tccgacttgt
     4501 acacgatcgc aaacatggcc aagcctcctg ttgcgggtga aaccaccggg gggcgtccgc
     4561 ccgggccaag tactttaacc catgtcctgc ggagcatcca gtgacgaaat cctcgatcga
     4621 ccgaatgaac tatgcagctc gcagcgcata atcgcgccgt cgccgcctgg ctgctcgcct
     4681 gctgcgcg
//
LOCUS       DASPZD010000337         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_162252, whole genome shotgun sequence.
ACCESSION   DASPZD010000337 DASPZD010000000
VERSION     DASPZD010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162252"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..426
                     /locus_tag="VEM33_06570"
     CDS             148..426
                     /locus_tag="VEM33_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51524.1"
                     /translation="MEIAQNHAAGAAAKLAKLCPPNNAPPVPQARRIAAAPAPPHGSA
                     HRLRSGFQLPGAIGHCSAKPMDMAGASSIIHANEHRSQERKGKNPTFP"
     gene            complement(695..>3223)
                     /locus_tag="VEM33_06575"
     CDS             complement(695..>3223)
                     /locus_tag="VEM33_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYS51525.1"
                     /translation="IAILLREEMQRRDSPFKVQIFATDIDNHALDIARHGIYPQGAVR
                     DLPEEKLNLYFTKQVNTYRIAKEIREMCIFSLHNLIKDTPFSRLDLISCRNLLIYFDP
                     ALQQRVLALFHFGLRQRGYLFLGPAENITPQPKLFSRVDSRARLFKARVVEPGRVPPE
                     LSLTVPRPDHVPQARSATPTAQEAATRRAQRVIEAYAPAAVLVDEHYEVLHFSGRTGK
                     YLQPSPGAASLNLFNIVDPGLRPDLRAAISRAMSNGKRIVRENLRIPADGGTLLMNIV
                     VEPLPAGDSEQRLCVVLFQEAAPATPEPRRDKRPDSKKNEIIQHLEAELVSTRERLQA
                     TIEELETSNEEMKSSNEEFQSVTEELQSSNEELETSKEELQSINEELETVNAELNSKV
                     ESLDRALSDQKNLLENTEIATIFLDSKLRIKSFTSPVTEIFRLIESDLGRPVTDISTR
                     LAYEHLPRDIARVLRTLSRVEHEVATIDGSASYIMRILPYRTVANVIDGVVVTFLDIT
                     ERKRHEEALAHLAAIVADSADAIIGLKPDGSIASWNAGAERIYGFTAEEAVGRPLSMT
                     MPPESEHELRQILERLRRSTPVSAFETGRITKDGRRIHVSYTSTPVRNQAGKLLAAAV
                     IERDITRRKHADEQQKMLLAELNHRVKNALATVLSIASRTRKVTDSLDEYYRAFEGRL
                     RALASTHDLLAKNIWAGAYLRQILLAELEPYKGGKETILISGRNLFVSSRAAVVFGMI
                     FHELATNAAKYGALSDTGGNVQVRWNMDGDKDAESFVLEWQERGGPTAGAAGKQGFGI
                     KFIERSVAYELQGSAELRFDPGGLHATIRAPLTEVMGEAGAPDS"
BASE COUNT          525 a         1044 c         1072 g          582 t
ORIGIN      
        1 gtgaccggct gccattccac gatcgcaagc ccggactcgg tcacccgcgt cgccccgcca
       61 acgagcacca tgacaaaaat cagtccggcg acggcgagca gccagatccg gaccatgcgc
      121 agatgccgag tttcggaaat cgggtccatg gagatcgcgc agaaccatgc ggcgggagcg
      181 gcggcgaaat tagccaagct ttgcccaccg aacaatgcgc cgcctgtacc gcaagcgcgg
      241 cggatcgccg ccgcgccggc gccgccccat ggctcggcgc accgcctccg ctccggcttc
      301 cagcttcctg gcgcgatcgg tcattgctcg gctaagccaa tggacatggc cggcgcatcg
      361 agcataattc acgccaatga gcacagaagc caagagcgaa aaggaaaaaa tccgacgttt
      421 ccgtgatcgg gcggaggaat gccgcgccca tgccgccgaa atgaaggacc cggacgctcg
      481 cgcagggctt atccaagttg cggaatccta tgagagaatg gccggcgcca tcgaggcgaa
      541 gcttcccgag gatactccgc ccaagtcgta gcgcgcaggg caggctgacc gggccgggtc
      601 ccggtcgcgc atgcgcgatg gggtgcccgg gaagcccagg cggaccggcc cagagacggc
      661 gggctgcgct tggcgtggcc cggcgcgcgc gcccttacga atccggcgct cccgcctcac
      721 ccatgacctc ggtcagcggc gcgcggatcg tcgcgtggag gccgcccgga tcgaagcgca
      781 gctcggcact accctgcagc tcgtacgcga cgctgcgctc gatgaacttg atgccgaagc
      841 cctgcttgcc cgcggcgccc gcggtagggc cgccccgctc ctgccattcg agcacgaaac
      901 tctcggcgtc cttgtcacca tccatattcc agcgcacctg cacgtttccg ccggtgtcgg
      961 agagcgcgcc gtatttcgcc gcattggtcg ccagctcgtg aaagatcatg ccgaatacga
     1021 ccgcggcgcg cgaggaaacg aacagattgc ggcccgagat caggatcgtc tccttccctc
     1081 ccttgtacgg ctcgagctcc gccagcagga tctgccgcag gtacgcgccg gcccagatgt
     1141 tcttcgccag caggtcgtgg gtgctcgcca gcgcgcgcag ccgcccctcg aaggcgcgat
     1201 aatattcgtc gagcgaatcc gtgaccttgc gggtgcgcga ggcgatcgac agcacggttg
     1261 cgagcgcatt cttgacccga tgattgagct cggcgagcag catcttctgc tgctcgtcgg
     1321 cgtgcttgcg ccgggtgatg tcgcgctcga tcaccgccgc ggcgagcaac ttgccggcct
     1381 ggttgcggac cggcgtcgag gtgtaggaga cgtggattcg tcgtccgtcc ttggtgatcc
     1441 gcccggtctc gaaggcgctg accggggtcg agcgccgcag gcgctcaagg atctggcgca
     1501 gctcgtgctc gctttcgggc ggcatcgtca tggaaagcgg ccggccgacc gcctcctccg
     1561 cggtaaagcc gtagatgcgc tcggctccgg cgttccagct ggcgatgctg ccgtccggct
     1621 tgagtccgat gatggcgtcg gcggaatctg ccacgatcgc ggccaggtgc gccagcgcct
     1681 cctcgtggcg cttgcgctcc gttatgtcca ggaacgtgac gaccacgccg tcgatcacgt
     1741 tggctacggt ccggtacggc aggatgcgca tgatgtagct ggcgctgccg tcgatcgtgg
     1801 ctacttcgtg ctcgacccgg gacagcgtgc gcagcacgcg ggcgatgtcg cgcggcagat
     1861 gctcgtaggc gagccgggtc gagatgtcgg tgaccggccg gccgaggtcg ctctcgatca
     1921 ggcggaagat ctcggtgacc ggcgaggtga agctcttgat gcgcagcttg ctgtcgagaa
     1981 agatcgtggc gatctcggtg ttctccagca ggttcttctg atcgctcagc gcgcggtcga
     2041 ggctctcgac cttggagttg agctcggcgt tcaccgtctc caattcctcg ttgatcgact
     2101 gcagctcctc cttggaagtc tcgagctcct cgttcgagct ttgcagctcc tcggtgacgg
     2161 attggaactc ctcgttcgag gatttcatct cctcgttcga ggtctcgagc tcctcgatgg
     2221 ttgcctgcag ccgctcccgg gtcgacacta actctgcctc gagatgctgg atgatttcgt
     2281 tcttcttcga atccgggcgt ttgtcgcgtc gcggttcggg tgtggccggg gccgcttcct
     2341 ggaacagcac cacgcacagg cgctgctcgc tgtcgccggc gggcagcggt tccaccacga
     2401 tgttcatcag cagcgtgccg ccatcggccg gaatccgcag gttctcgcga acgatcctct
     2461 tgccgttgct catggcccgg ctgatcgcgg cgcgcagatc cggccgcagg ccgggatcga
     2521 cgatgttgaa caaattgagg cttgcggcgc cggggctcgg ctgcagatat ttgccggtcc
     2581 gcccggagaa atgcaggact tcgtaatgtt cgtcgacgag caccgccgcg ggcgcatagg
     2641 cctcgatcac ccgctgcgcg cgccgggtcg ccgcctcctg ggccgttggc gtagccgacc
     2701 tggcctgcgg gacgtggtcg ggccgcggta ccgtgagcga cagctccggc ggaacgcgcc
     2761 cgggctccac gacgcgcgct ttgaacaggc gcgcgcggga atccaccctg gagaacagct
     2821 tgggctgcgg cgtgatgttc tcggcgggcc ccaggaacag atatccgcgc tggcgcaagc
     2881 cgaaatggaa cagcgcaagc acccgctgct gcagggccgg gtcgaaatag atcaggagat
     2941 tgcggcagga gatcaggtcg agcctcgaga acggtgtgtc cttgatgaga ttgtgcagcg
     3001 agaagatgca catctcgcgg atctccttgg cgatgcggta cgtgttcacc tgcttggtga
     3061 aatacaggtt cagcttctcc tccggcaggt cgcgcacggc cccctgcgga tagatgccgt
     3121 ggcgcgcgat atccagggcg tgattgtcga tgtcggtggc gaagatctgc accttgaacg
     3181 gcgaatcccg gcgctgcatt tcctcgcgca ggaggatggc gat
//
LOCUS       DASPZD010000338         3868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_10857, whole genome shotgun sequence.
ACCESSION   DASPZD010000338 DASPZD010000000
VERSION     DASPZD010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10857"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VEM33_06580"
     CDS             <1..750
                     /locus_tag="VEM33_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HYS51526.1"
                     /translation="EEIERTLKCGPGYIGPVSSVPLSLYADRTVAAMSDFVCGANEAG
                     FHLTGVNWGRDLPEPGHTADIRKVVAGDPSPDGKGILEIVRGIEVGHIFQLGTKYSQA
                     MKATFLDEAGNSRFLEMGCYGIGITRIVGAAIEQNHDEHGIVFPEPIAPFHVALVPIG
                     YGKNASVREAADSIYRALTDAGFEVLLDDRDERPGVMFADMELIGIPHRIVVGERGLK
                     DGLIEYQGRVDKETKKFPLGDPLAPLRTGSC"
     gene            744..1370
                     /locus_tag="VEM33_06585"
     CDS             744..1370
                     /locus_tag="VEM33_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYS51527.1"
                     /translation="MLKGAGLALIGIAFPGLALAGAQIYEPLAREVRAGLQASIADKP
                     APRHAFADSESAVAWLDEMSHRLEARMTNFQSRVEFLRTVHFEANRAKLDPQLVLAVI
                     QVESNFRKYAVSRSGARGYMQVMPFWVGLVGRPGDNLFSLRNNLRYGCVILKYYLDRE
                     KGDLFRALSRYNGSFGRPEYPNMVIGAWRSVWRYEGKLAAAPASPDAG"
     gene            complement(1383..2090)
                     /gene="pyrF"
                     /locus_tag="VEM33_06590"
     CDS             complement(1383..2090)
                     /gene="pyrF"
                     /locus_tag="VEM33_06590"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYS51528.1"
                     /translation="MKTIPRAERLIAALDVAGVEEAKALVQRLGDSVSFYKVGLELFM
                     AEGFFGFIDWLARTGKKVFVDLKFFDIPETVRAAVRGLGNRGVTFATVHGNQAMLEAA
                     AQAKGDVKILAVTVLTSLDQGDLDDLGFQCDVQTLVLSRARRALEAGCDGVVSSGLEA
                     PVLRRELGARLLIITPGIRPVQNRLIGDQKRTVDVARAFANGADYIVVGRPIREAPDP
                     RAAAQAIQATIATEFPG"
     gene            complement(2130..3344)
                     /locus_tag="VEM33_06595"
     CDS             complement(2130..3344)
                     /locus_tag="VEM33_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HYS51529.1"
                     /translation="MPFPLRQLGRFLAHVFKRFNEDRCLQIASSLTFTTLLALVPLVT
                     LTLALMAAFPIFSGLGEHIHAFLLANMLPETAGKVVTGYIEQFSGQAGRLTALGSAVL
                     AVTAFLMMFTIERAFNTIWRVSRPRPLAQRILIYWATLTLGPVLIGASLSITSYLVGV
                     SLGFARQSPFAGAAILGLVPFVLTCAAFTLLYYFVPNRAVKPRHALIGGLVAALVFEV
                     MKRSFALYIAKIPTYTLVYGAFAVIPVFLLWVYFSWVVIVIGALIAALAPDFTVLRET
                     ARRPSGAGFGDVLEILLILARAQQGPELLELRKIAQDASLTVDQTERILERLVARGWV
                     ARSSGERYAMVVDKERLLVADVYREFVLDGEGPGERGRSEPLKRLLGQFAARVDETLN
                     LPLGRLLERDRG"
     gene            complement(3392..>3868)
                     /locus_tag="VEM33_06600"
     CDS             complement(3392..>3868)
                     /locus_tag="VEM33_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HYS51530.1"
                     /translation="AYNKAFQRFKDLADKKADIFDEDLQALVSDGATAAAAERWQLVR
                     MSQASGTGERPHAKLVLAEAGKERQTDAEGDGPVDATFKAIERVAKSGAELLLYSVNA
                     VTQGTESQGEVTVRLAKGGRIVNGVGADTDIIVASAKAYVNALNKLQGAQRINPQG"
BASE COUNT          741 a         1238 c         1214 g          675 t
ORIGIN      
        1 gaggagatcg aacggacatt gaagtgcggc cccggctaca tcggaccggt gagcagcgtg
       61 ccgttgagcc tctacgccga tcgcacggtg gcggcaatga gcgatttcgt gtgcggtgca
      121 aacgaggcgg gattccactt aacaggcgtc aactggggac gtgaccttcc ggagcccggt
      181 catacagcag acatccgcaa ggttgttgcc ggcgatccca gccccgacgg gaaggggatt
      241 ctggaaatcg tccgggggat cgaggtcgga catatcttcc agctggggac gaagtactcg
      301 caagcaatga aggcgacgtt cctcgacgag gcgggcaaca gccgcttcct cgagatgggg
      361 tgctatggaa tcggcatcac ccgcatcgtg ggcgccgcga tcgagcagaa ccacgacgag
      421 cacgggatcg tgttcccgga gccgatcgcg ccgttccatg tcgccctggt tcccattggc
      481 tacggcaaga acgcctccgt ccgcgaagcc gccgactcga tctatcgcgc gctcaccgat
      541 gccggtttcg aggtgctgct cgacgatcgc gacgagcggc cgggcgtgat gttcgcagac
      601 atggagctca tcggcattcc ccaccgcatc gttgtcggcg agcgcggtct caaggacggc
      661 ttgatcgaat atcaaggccg ggtcgacaag gagacgaaga agttcccgct cggcgatccg
      721 cttgccccgc tgcgcacggg ctcatgctga aaggcgccgg cctggcgctg atcgggatcg
      781 cgttcccggg gcttgctctc gcaggagccc agatctatga accgctcgcc cgcgaggtgc
      841 gggcgggcct gcaggcctcg atagcggaca agccggcgcc gcgccatgcc tttgccgact
      901 cggagtcggc agtcgcctgg ctcgacgaaa tgtcgcatcg cctggaagcg agaatgacga
      961 acttccagtc gcgcgtggaa ttcctgcgca cggtgcattt cgaagcgaat cgcgcgaagc
     1021 tcgacccgca gctcgtgctg gcggtgatcc aggtcgagag caattttcgc aaatacgccg
     1081 tgtcacgctc gggtgcccga ggctacatgc aggtcatgcc cttctgggtc ggattggtcg
     1141 gccgtccggg cgacaacctg ttcagtctgc gcaacaacct tcgctacggc tgcgtcatcc
     1201 tgaagtacta cctcgaccgt gaaaaagggg atctctttcg cgccctgagt cgatataacg
     1261 gatccttcgg caggcccgag tatcccaaca tggtgatcgg cgcctggcgc agcgtgtggc
     1321 gctacgaagg caagcttgcc gcggctcccg cctcgcctga cgccggctag cccgtccgga
     1381 tatcagccgg gaaactcggt cgcaatcgtc gcctgaatcg cttgcgccgc ggcacgagga
     1441 tcgggcgcct cgcgaatcgg gcggccgacg acgatgtagt cggcgccgtt cgcgaaagcc
     1501 cgcgccacgt cgaccgtgcg cttctggtcg cctatgagcc gattctgcac cggtcgtatt
     1561 cccggcgtga tgatcagcag ccgcgcaccc agctcgcgcc tcagcaccgg cgcctccagc
     1621 cccgaggaaa cgaccccatc gcagccggcc tcgagcgccc gtcgcgcccg cgagagcacc
     1681 agagtctgta catcgcattg aaaaccgagg tcgtcgagat cgccctgatc gaggctggta
     1741 agaaccgtga ccgcgaggat ttttacgtcc cccttggcct gtgccgccgc ctcgagcatc
     1801 gcctggttgc catgtacggt cgcgaaggtc acgccccgat tgccaagccc gcgcacagcg
     1861 gcccgaaccg tctcagggat gtcgaaaaac ttgaggtcga caaacacctt cttccccgtt
     1921 cgcgcaagcc aatcgatgaa gccgaagaat ccttccgcca tgaacaattc caggccgacc
     1981 ttgtagaagc tcacgctgtc tccaaggcgc tgtacaaggg ctttcgcctc ttcgacgcct
     2041 gcgacgtcca aggctgctat caggcgctcg gcgcgcggga ttgtcttcat cggcgatgtc
     2101 atggcgttcg gagcgaggat tctaacggat caacccctgt ccctttcaag caggcgcccg
     2161 aggggcaggt tcaaggtttc gtccacccgc gcggcaaact gccccaggag tcgcttcagg
     2221 ggttcgctgc gccctcgctc tcctggccct tcgccatcca gaacgaactc gcgatacacg
     2281 tcggccacga ggaggcgttc cttgtcgacg accattgcgt agcgctcgcc ggaggacctc
     2341 gccacccagc cacgcgcaac taggcgttcg agtatgcgct cggtctgatc caccgtgagc
     2401 gatgcgtcct gggcgatttt cctcaattcg agaagctccg gaccttgctg ggcgcgcgcc
     2461 agaatcagaa gaatctccag cacgtcgccg aagccggctc ccgacgggcg ccgggctgtt
     2521 tcgcgcagca cggtgaaatc cggcgccagc gcggcaatca acgcaccgat cacgatcacc
     2581 acccaagaaa aatagaccca gaggagaaag accgggatca ctgcgaatgc gccgtagacc
     2641 agcgtatagg tggggatttt cgcgatgtag agggcgaagc ttcgcttcat gacctcgaac
     2701 accaaggccg cgaccaggcc tccgatcagg gcgtggcgcg gcttgaccgc gcggttcggc
     2761 acgaaatagt agagcagcgt gaacgctgca caggtcagaa cgaatggaac gagtccgaga
     2821 atcgccgcac ctgcaaaagg actctgccgc gcgaatccga gggatacacc gacgaggtac
     2881 gaagtgatcg aaaggctcgc cccgatgaga accggcccga gggtgagcgt tgcccaatag
     2941 atgaggatgc gctgcgccaa gggacgcggg cgtgaaaccc gccagatcgt gttgaacgcg
     3001 cgctctatcg tgaacatcat gaggaaggca gtgacggcga gcaccgcgga accaagcgcg
     3061 gtcaaccgcc cggcctgacc cgagaattgt tcaatgtatc cggtgacgac ctttcctgcc
     3121 gtctcgggca acatgttcgc gagcaggaac gcatggatgt gctcccccag ccccgaaaaa
     3181 atcgggaacg ccgccatgag cgcaagcgtg agggtgacaa ggggcacgag cgcaagcaag
     3241 gtcgtgaaag tgagactcga tgcgatttgt aaacagcggt cctcgttgaa ccgcttgaac
     3301 acgtgcgcaa ggaagcggcc cagctgtcgc agcggaaagg gcatgggatc cctgaatcgg
     3361 agagtgaacg tctcctatga tataggggcc ctcacccctg cgggttgatt cgctgggcgc
     3421 cctgtagctt gttgagtgca ttgacgtagg ccttggccga agcgacgatg atatcggtgt
     3481 cggcacctac gccgttcact atcctgccgc ctttcgcgag gcgcacggtc acttcgccct
     3541 gcgactcggt cccctgcgtt acggcattca ctgagtacag cagcagctcg gcgccgctct
     3601 tggccacgcg ttcgatcgcc ttgaaagtcg catccaccgg gccgtcgccc tcggcgtcgg
     3661 tctggcgctc cttgccggcc tcggcgagca cgagcttcgc gtgcggacgc tcgccggtgc
     3721 cgctcgcctg gctcatgcgc accaactgcc atcgctccgc cgcggcggcg gtcgcaccgt
     3781 cggagacgag cgcctgcagg tcctcgtcga aaatgtcggc cttcttgtcg gcgaggtcct
     3841 tgaagcgctg gaacgccttg ttgtaggc
//
LOCUS       DASPZD010000339         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_514999, whole genome shotgun sequence.
ACCESSION   DASPZD010000339 DASPZD010000000
VERSION     DASPZD010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514999"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3129)
                     /locus_tag="VEM33_06605"
     CDS             complement(<1..>3129)
                     /locus_tag="VEM33_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HYS51531.1"
                     /translation="GAIRPWQSPSFNECQDDLEKYAKKRGVPLDVPFRDLSAKERVWV
                     LDGEPEWVSWRKSWPGTWYGVARFFRWLETKAYKMHVRVLLSRYRAYTPCSACKGARL
                     KPEALLWRVGTKEDADRVLDPARRFRPAGTDFGDEVLRALPGLTVHDLVLLPAERTRA
                     FFEGLHLPAPLDEATDLLLTEIRARLRYVQEVGLGYITLDRQSRTLSGGEVQRINLTT
                     ALGTSLVNTLFVLDEPSIGLHPRDMGRVIGVMQRLRDAGNSLVVVEHDPQIMFAADRI
                     MDMGPGPGERGGEIVFFGTPDELKQSKTLTAEYLSGRKRAPKPGTDHGFPENRGLSPV
                     LELLGAAEHNLKAIDVGIPLQRLVCITGVSGSGKSTLVQDVLYAALRKAKGKPTETPG
                     TFRELRGHGLIEDVVMVDQTPIGRTTRSNPASYVGAWNAIRNIYAKEPFAKERRYTPG
                     TFSFNSGNGRCPTCGGNGFEHVEMQFLSDVYLRCPDCNGRRFRDEVLEVVVEGKSIAD
                     VLELTVSEALAFFRDHPEVQRALKPLADVGLEYLRVGQPVPTLSGGEAQRLKLAGHLV
                     ESASDSAGGERGRLFLFDEPTTGLHFDDVAKLLSAFRQLIAAGHSLFVIEHNLDVIRA
                     SDWLIDLGPEGGDAGGAIVCTGTPEEVAKHASSHTGKALREYDAAFAKPAALRAAEPG
                     VPLQVRVSQSIRIHNAREHNLKSIDVEIPRNEFTVVTGVSGSGKSTLAFDILFAEGQR
                     RYLESLNAYARQFVQGAARPDVDAIFGIPPTVAIEQRTSRGGSKSTVATLTEIYHFLR
                     LLYVKLGLQYCPDCQVPIEPQSEDAIAARLLREYKGQRIGLLAPLVVNRKGYYTDLAK
                     WARGKGYEFLRVDGEFLPTGKWPRLSRFQEHTLEIPVADLRISADNETEIRRALALAL
                     EFGKGVVHVLAGLDRLEEAKANSRSTRVEMDLPTRIFSTKRACPSCGRSFAELDPRLF
                     SFNSKHGWCPECYGTGLKLDKVEWDEEREKTGTEDHVLDSWIEWLEVDESCPACDGKR
                     LNGEALAVRYQER"
BASE COUNT          501 a         1014 c         1069 g          545 t
ORIGIN      
        1 cgctcctggt aacgtaccgc gagcgcctcg ccgttgaggc gcttgccgtc gcaggcaggg
       61 cagctttcgt ccacctccag ccactcgatc caggaatcga gcacgtgatc ctcggtaccg
      121 gtcttctcgc gttcctcgtc ccattcgacc ttgtcgagtt tcaggccggt accgtagcac
      181 tccgggcacc agccgtgttt ggagttgaac gagaacagcc gcggatcgag ctcggcgaag
      241 ctgcggccgc acgaggggca ggcgcgtttc gtggagaaga tgcgggtcgg caggtccatt
      301 tccactcgcg tggaccggga attcgccttg gcctcttcca gacggtcgag ccccgcgagc
      361 acatgcacta cgcctttgcc gaattccaga gcgagggcga gcgcgcgacg gatttcggtc
      421 tcgttgtccg cagagatgcg cagatcggcg accgggatct ccagcgtgtg ctcctggaag
      481 cgcgacagcc gtggccattt gccggtcggg aggaactcgc cgtcgacgcg caggaattcg
      541 tagcccttgc cgcgtgccca cttggcgagg tcggtgtaat agcccttgcg gttgacgacc
      601 agtggcgcga gcaggccgat gcgttgcccc ttgtattcgc gcaggaggcg cgccgcgatc
      661 gcgtcttcgc tctgcggctc gatcggcacc tggcagtcgg ggcagtactg gagcccgagc
      721 ttgacgtaga gcaaccgcag gaagtgatag atctcggtca gcgtcgcgac ggtgctcttg
      781 cttccgccgc ggctagtgcg ctgctcgatc gcgaccgtgg gcggaatgcc gaagatcgca
      841 tcgacgtcgg gacgcgccgc tccctggacg aactggcgtg cgtaggcgtt gagcgactcg
      901 agatagcgcc gctggccttc ggcgaaaagg atgtcgaaag cgagcgtgga ctttcccgag
      961 cccgagacgc cggtcaccac ggtgaactcg ttgcgcggga tttcgacgtc gatggatttc
     1021 aggttgtgct cgcgagcatt gtgaatcctg atcgactggc tgacgcggac ctgcagcggc
     1081 acaccgggct ccgccgcgcg aagggcggcg ggctttgcga acgcggcatc gtattcgcgc
     1141 agcgccttgc ccgtgtgcga gctggcgtgc ttcgcgacct cctccggcgt gccggtgcag
     1201 acgatcgcgc cgccggcgtc gccgccctca gggccgaggt cgatgagcca gtcggaggcg
     1261 cggatgacat ccagattgtg ctcgatcacg aacagcgagt gtcccgcggc gatcagctgg
     1321 cggaaggcgg agagcagttt cgccacgtcg tcgaagtgaa ggccggtggt cggctcgtcg
     1381 aagagaaaca acctgccccg ttccccgccc gcggagtcgc tcgcggattc gaccaggtgt
     1441 ccggcgagct tcagccgctg cgcttcgccg ccggagagcg tcggcaccgg ctggccgacg
     1501 cgcaggtatt cgagccctac gtccgccagc ggcttcaatg cgcgctgcac ctcgggatgg
     1561 tcgcggaaga acgcgagcgc ctcggacacg gtcagctcga gcacgtcggc gatcgacttc
     1621 ccttccacta cgacctcgag cacttcatcg cggaagcgcc tgccgttgca gtcggggcag
     1681 cgcaggtaga cgtcggagag gaactgcatc tccacatgct cgaagccgtt gccgccgcag
     1741 gtcgggcagc gcccgttgcc ggagttgaag ctgaaggtgc ccggggtgta tctgcgttcc
     1801 ttcgcgaacg gctccttcgc gtagatgttg cggatggcgt tccaggcgcc cacgtagctc
     1861 gccgggttcg agcgcgtcgt gcgcccgatc ggggtctggt ccaccatcac cacgtcctcg
     1921 atcagaccgt ggccgcgcag ctcgcggaac gtgcccggcg tttcagtcgg cttgcccttc
     1981 gccttgcgaa gcgctgcgta gagcacgtcc tgcaccagcg tggatttgcc cgagcccgag
     2041 acgccggtga tgcacaccag gcgctgcagc ggaatgccga cgtcgatggc cttcaggttg
     2101 tgctcggccg cgccgagcag ctccaaaacc ggggacagac cacggttttc cggaaaaccg
     2161 tggtctgtcc ccggttttgg agcgcgcttc ctgcccgaga gatattccgc cgtgagcgtt
     2221 ttcgactgct tcagctcgtc aggcgtgccg aagaacacga tttctccccc gcgctcgccg
     2281 gggccgggac ccatgtccat gatgcggtcg gcggcgaaca tgatctgcgg atcgtgttcg
     2341 acgaccacga gcgagtttcc ggcgtcgcgc aggcgctgca tcacgccgat gacgcgaccc
     2401 atgtcgcgcg ggtgcagccc gatcgagggc tcgtccagca cgaacagcgt gttgacgagc
     2461 gaggtgccga gcgcggtggt gaggttgatg cgctgaacct cgccgcccga gagcgtgcgc
     2521 gattggcggt cgagcgtgat ataacccagg ccgacttcct ggacgtagcg cagccgcgcg
     2581 cggatctcgg tgagaaggag gtccgtcgcc tcgtccaagg gcgctggaag gtgcaggcct
     2641 tcgaagaagg cgcgcgtgcg ctcggcgggc agaagcacga ggtcgtgcac ggtgaggccc
     2701 gggagggcac gcaatacctc gtcgccgaag tccgtgcctg ccggcctgaa cctgcgcgcc
     2761 ggatcgagca cgcgatcggc gtcctccttg gtcccgacgc gccacagcag cgcctcgggt
     2821 ttcagccgcg cccccttgca ggccgagcag ggcgtgtagg cgcggtagcg cgacaggaga
     2881 acgcgcacgt gcatcttgta cgccttggtc tcgagccagc ggaaaaagcg cgccacgccg
     2941 taccaggtgc ccggccagga ctttctccag ctcacccatt cgggctcgcc gtcgagcacc
     3001 cagacacgct ctttcgcgga aagatcgcgg aagggaacgt cgagcggcac gccgcgcttc
     3061 ttcgcgtact tctcgagatc gtcctggcat tcgttgaaag agggcgactg ccagggccgg
     3121 atcgcgccc
//
LOCUS       DASPZD010000340         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_11683, whole genome shotgun sequence.
ACCESSION   DASPZD010000340 DASPZD010000000
VERSION     DASPZD010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11683"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..2013
                     /locus_tag="VEM33_06610"
     CDS             211..2013
                     /locus_tag="VEM33_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYS51532.1"
                     /translation="MEPTNIAAPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYGDAY
                     MLNWKSNVFPGILGRTCDRPCEPACRRGRVENEPVAICRLKRVAADYKGDIKARLPRP
                     PQHKNGKRIALVGGGPASLTVARDLAPLGYEVTVFDQDPRAGGMIWSQIPRFRLPVEV
                     IDEEVGYILDLGVEFKGGVKIDSMKKLIEQSYDAIFVGSGAPRGRDLDIPGRREAAKN
                     IHIGIDWLSSVSFGHTDKIGRRVIVLGGGNTAMDCCRSSKRLGGEDVKVIVRSGFEEM
                     KASPWEKEDAQHEGIPILNYLVPKTFEHENGKLTGMTFEKVKALRDEKGRRSLVPTGE
                     PDQRFECDDVLVAVGQENAFPWIERDAGIQFDQHGMPVVDKVTMQSTQAKVFFGGDSA
                     FGPKNIIWAVAHGHDAAISIDKLLNGEDLKERPSPIVNLSSQKMGIHEWSYDNAISPD
                     ERYKVPWKDKAITLKNIKVEVELGFDPMTGFKEAQRCLNCDVQTVFADKLCIECDACV
                     DICPMDCITFTQNGEEADLRTRLTAPARNPTQDIYVSGPLKTGRIMAKDEDVCLHCGL
                     CAERCPTGAWDMQKFLLDLTHAGHGCRTPQKKAA"
BASE COUNT          415 a          655 c          659 g          334 t
ORIGIN      
        1 taccgcgctg caaaaacatt ggccacgcct gttaatttcg cgtcaggcgc ttcaccacaa
       61 ttctgccatg ccagttgcgg cagtagagcc gatcgtctat gcggattacg ttacagacgg
      121 atcggctgac cgaaaagacc gaccggagga aatggctcgc ttccgcagcc gggcggggtg
      181 tggcctgcgt gtttgcagca cgaggagtga atggaaccca ccaacattgc ggccccggac
      241 tatttccaca aggtcgtcga ttgccagtgg gcctgtccgg cgcacacccc cgttccggaa
      301 tacatccgat tgatcgcggc cgggcgttat ggcgacgcct acatgctcaa ttggaagtcc
      361 aacgtgtttc ccggcattct cggccgcacc tgcgaccggc cgtgcgagcc cgcctgccgg
      421 cgcggccgcg tcgagaacga gccggttgcg atctgccggc tcaagcgcgt cgccgcggac
      481 tacaaaggcg acatcaaggc gcgcctgccg cgcccgcccc agcacaagaa cggcaagcgg
      541 atcgcgctgg tgggcggcgg ccccgcctcg ctcaccgtcg cgcgcgatct cgcgccgctc
      601 ggctatgaag tcaccgtctt cgaccaggat ccgcgcgccg gcggcatgat ctggtcgcag
      661 atcccgcgct tccgcttgcc ggtggaagtg atcgacgagg aggtcggcta catcctcgat
      721 ctgggcgtcg agttcaaggg cggtgtgaaa atcgacagca tgaaaaagct gatcgagcag
      781 agctatgacg cgatcttcgt cggctctggc gcgccgcgcg ggcgcgatct cgacattccc
      841 gggcgcaggg aagcggcgaa gaacatccat atcggcatcg actggctttc ttccgtttcc
      901 ttcggccaca ccgacaagat cggcaggcgc gtgatcgtgc tgggcggcgg caacaccgcc
      961 atggactgct gccggtcctc gaagcgcctc ggcggcgagg acgtgaaggt gatcgtgcgc
     1021 tcggggttcg aggagatgaa ggcttcgccc tgggagaagg aggacgcgca gcacgaaggc
     1081 attcccatcc tgaattacct cgtacccaag accttcgagc acgagaacgg aaaactgacg
     1141 ggcatgacct tcgagaaggt caaggcgctg cgtgacgaaa aaggccggcg cagcctcgtc
     1201 cctaccgggg aaccggacca gcgcttcgag tgcgatgatg tcctggtcgc ggtcgggcag
     1261 gagaacgcct ttccctggat cgagcgcgac gccggaatcc agttcgacca gcacggcatg
     1321 ccggtcgtgg acaaggtcac catgcagtcg actcaagcca aggtcttctt cggcggcgac
     1381 tcggcgttcg gccccaagaa catcatctgg gcggtagccc acgggcacga cgccgcgatt
     1441 tcgatcgaca agctcctgaa cggggaggac ttgaaggaac gcccgtcgcc gatcgtaaac
     1501 ctctccagcc agaagatggg cattcacgag tggagctacg acaacgcgat ctcgcccgac
     1561 gagcgctaca aggttccctg gaaggacaag gcgatcacgc tgaagaacat caaggtcgag
     1621 gtcgagctcg gcttcgaccc gatgaccggc ttcaaggaag cgcagaggtg cttgaactgc
     1681 gacgtgcaga cggtgttcgc cgacaagctg tgcatcgagt gcgacgcctg cgtggacatc
     1741 tgcccgatgg actgcatcac cttcacccag aacggcgagg aagcggacct gcgcacgcgc
     1801 ctcaccgcgc cggcgaggaa tcccacgcag gacatctacg tctcggggcc gctgaagacg
     1861 ggcaggatca tggcgaagga cgaggacgtg tgcctgcact gcggcctgtg cgccgagcgc
     1921 tgcccgaccg gcgcctggga catgcagaag ttcctcctcg acctgacgca cgccgggcac
     1981 ggctgccgca cgccccagaa gaaagccgcc tgaatccgat ggtaaagcgc atcgaatccg
     2041 tcaacgcctt cgtcgtcaag ttc
//
LOCUS       DASPZD010000341         5233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_432684, whole genome shotgun sequence.
ACCESSION   DASPZD010000341 DASPZD010000000
VERSION     DASPZD010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432684"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VEM33_06615"
     CDS             complement(<1..1193)
                     /locus_tag="VEM33_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS51533.1"
                     /translation="MLLHLSIRDFAIVDRLELEFRSGFTALTGETGAGKSILIDALSL
                     TLGDRAGSEQVRSGAERADVTADFAIDSLPGIGDWLREQALEGDPSRLLLRRVVDASG
                     RSRAFINGHAVTINQMREVGDRLVDIHGQHAHQSLLRPEAQRLVLDSHAGLASLAEET
                     GGAHREWQRLCRARLDHQNNAAARDAEREQLGWQLEELSRLALAPGEWEAIQSEHGRL
                     AHAATLMEGVRDAIDSLSESENAALGVLSGVLSRLRPLTEFDGSLRDTIAVLESGEAQ
                     LREAAYALRHYADRIELDPKRLHEVEQRLEAVHNSARKFRTRPEALPELLASLRVRMK
                     DLEMTADLEALAAQEQAARLRYDELAARLSAERSKAATKLTREVNAAMKELAMSGGRF
                     EVELRS"
     gene            complement(1200..2069)
                     /locus_tag="VEM33_06620"
     CDS             complement(1200..2069)
                     /locus_tag="VEM33_06620"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HYS51534.1"
                     /translation="MSAFKTIALIGRYHSPDVATALVTMGEYLRKQGRAVLVEKETAA
                     TNRVNGFPVADYDEIGARADLVVVQGGDGSMLNAARNLAAHHVPLVGVNQGRLGFMTD
                     IASPHMLDAMADILAGRHAIEERTMLAAEVRRDGVVTLATLALNDAVVNKGSVGRLIE
                     FVVNIDGEFVYDLRADGLIVATPTGSTAYALSSDGPILQPSVPGFALVPICPHTLSNR
                     PIMVSDRTVIEVFLKRSIDARLHFDGQLQCDLQEGDRVLIRRAEHTVKFVHPPGYSYF
                     AMLRQKLHWSEVL"
     gene            2170..3186
                     /gene="hrcA"
                     /locus_tag="VEM33_06625"
     CDS             2170..3186
                     /gene="hrcA"
                     /locus_tag="VEM33_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HYS51535.1"
                     /translation="MDRRAQILLKTLVERYIAEGEPVGSRALSRYSGLDLSPATIRNV
                     IADLEELGFVASPHTSAGRIPTPRGYRFFVDTLLTVKQLDRVEISQLEDNLHPDHPQG
                     LISAASQLLSELTHFAGVVVASKRKAPAFRHIEFLALSDKRVLLIIVTPDGDVQNRIL
                     FTEKAYTPPELTMAANFLNQNYAGLTFADIRRRLRDELKQLREDMTQLMTAALEASSE
                     ALSESAENYVISGERNLLDSHELASNMSRLRELFELFEKKTLLVQILELSTHAQGVQI
                     YIGGESGVAPLDECSVVTAPYETDGQIIGTVGVIGPTRMAYERVIPIVDITAKLLSSA
                     LSSH"
     gene            3261..4349
                     /gene="hemH"
                     /locus_tag="VEM33_06630"
     CDS             3261..4349
                     /gene="hemH"
                     /locus_tag="VEM33_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HYS51536.1"
                     /translation="MHYLPEPKQEYGAIAKTAVLLVNLGTPDAPTPQAVRRYLKQFLS
                     DPRVIEIPRAFWLPILHAFVLNSRPRKSARKYASIWSPEGSPLKVHTERQARLLRGYF
                     VSQARMPLSVDFAMRYGEPSIQGTLARLKSEGCERVLVLPMYPQYAASTTASTLDELG
                     GFLRRVRNVPEVRLVKHFNDHPSYIAALANLVREHWRQAGRPDTLLMSFHGLPRYTLA
                     RGDPYHFECQKTARLLAEGLELAESQWQIAFQSRFGRAEWIQPYTASTLADYGRRGMR
                     RVDVICPGFVADCLETLEEIGIEGRKIFLGAGGREFNLLPCLNERDDWIRALADIARQ
                     HLSGWVMESGNPRLEKNRAEDPSRADSS"
     gene            4440..5006
                     /gene="grpE"
                     /locus_tag="VEM33_06635"
     CDS             4440..5006
                     /gene="grpE"
                     /locus_tag="VEM33_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYS51537.1"
                     /translation="MPEPDQPSPQVSSGDASVDPDAAIAKPADAAPAPDTEELLRQAE
                     SKAQEHHDAWLRAKAEAENIRKRSAIELANAYKYAIEAVGADLLPVKDSLEATLAADS
                     AALETLRSGVELTLKQLNAVFAKAALKEINPIGEKFDPHRHQAISMLPSEKEANTVIN
                     VLQKGYSLHDRVIRPALVTVAKPKDDRA"
     gene            5072..>5233
                     /locus_tag="VEM33_06640"
     CDS             5072..>5233
                     /locus_tag="VEM33_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYS51538.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGKPKVIENSEGARTTPSIVAYME
                     DGEILVGAPA"
BASE COUNT         1000 a         1702 c         1634 g          897 t
ORIGIN      
        1 gagcgcagct ccacctcgaa cctcccgccg gacatcgcga gctccttcat cgccgcgttc
       61 acctcgcggg tgagtttcgt cgcggccttg ctgcgttcgg ccgaaagccg cgccgcaagc
      121 tcgtcgtagc gcaagcgtgc agcctgctcc tgcgcggcca gggcttcgag atccgccgtc
      181 atttccagat ccttcatccg cacccgaagg gaagccagaa gctcaggcaa cgcctccggc
      241 cgtgtgcgaa acttgcgcgc cgaattgtgc accgcttcga ggcgctgctc gacctcgtgg
      301 aggcgcttcg gatcgagctc gatgcgatcg gcgtaatgtc ttagcgcata ggcggcttcg
      361 cgcagctgcg cttcgcccga ttcgagcacg gcaatggtgt cgcgcagcga tccgtcgaat
      421 tcggtgagcg ggcgcagccg tgaaagcact ccggacagca cgccgagcgc cgcgttctcc
      481 gactcggaaa gcgagtcgat cgcgtcccgc acgccctcca tgagggtcgc cgcgtgggcg
      541 agccgtccgt gctccgactg gatcgcctcc cattcgccgg gcgcaagtgc cagccgcgac
      601 agctcctcga gctgccaacc gagctgctcg cgctcggcat cgcgcgccgc cgcgttgttc
      661 tggtggtcga gtctcgctcg gcaaagcctt tgccattcgc gatgagcgcc accggtttcc
      721 tcggcgagcg aagcaagccc ggcatgcgag tcgagtacca ggcgctgggc ctcgggcctg
      781 agaagcgact ggtgcgcatg ctgaccgtgg atgtcgacga gcctgtctcc cacttcgcgc
      841 atctggttga tcgtcacggc gtgaccgttg atgaatgctc gcgagcgccc gcttgcgtcc
      901 acgacacggc gcaacagcaa ccggctcgga tcgccctcga gcgcttgctc gcgcagccaa
      961 tccccgatcc cgggcaggga atcgattgcg aaatccgcgg tcacgtcggc gcgttcggct
     1021 ccggaacgca cctgttcgct ccccgcgcga tcgccgagcg tcaaggacaa cgcgtcgatc
     1081 aggatggatt ttccggcacc ggtttcgccg gtgagtgcgg tgaaaccgga ccggaactcg
     1141 agttccaggc gatcgacgat cgcgaagtcc cggatcgaaa gatgcagaag cattcgcagc
     1201 tagagcactt cgctccagtg cagcttctgc cgcaacatcg cgaagtagct gtaacccggg
     1261 gggtggacga acttgacggt atgctccgcg cgccggataa ggacgcggtc accctcctga
     1321 aggtcgcatt gcagttgccc gtcgaaatga agccgcgcgt cgatcgagcg cttcaggaag
     1381 acttcgatca cggtgcggtc gctgaccatg atcggcctgt tggacagcgt gtgcgggcag
     1441 atgggcacca gcgcgaaccc gggcacgctc ggctgcagga tcggcccgtc agatgaaagc
     1501 gcgtaagcgg tcgacccggt gggggtcgcc acgatcaggc cgtcggcccg caggtcatag
     1561 acgaactcgc cgtcgatgtt caccacgaat tcgatcagcc gaccgacaga tcccttgttc
     1621 acgaccgcgt cgttgagagc gagggtcgcg agcgtgacca cgccatcgcg gcgcacttcg
     1681 gccgcgagca tggttcgctc ctcgatcgcg tgccggccgg cgagaatgtc agccatcgcg
     1741 tcgagcatgt gaggggaagc gatgtcggtc atgaatccga gacgtccctg gttgacgccc
     1801 acgagcggta cgtggtgggc cgccaggttt cgcgccgcgt tgagcatgct gccgtcgccc
     1861 ccctgcacca cgaccaggtc ggcgcgggcg ccgatttcat cgtagtccgc tacaggaaaa
     1921 ccgttgacac ggttggtggc cgcggtttcc ttctccacca gcactgcacg cccctgcttg
     1981 cgcaagtact cgcccatcgt cacgagcgcc gtcgcgacat cgggggagtg atagcggccg
     2041 atcaaggcaa tggttttgaa agcggacata ctgtcattaa atcatgaacg tgtggggtga
     2101 ccaaagggtg gcctgcaatg ccacgaacaa aacgcaaatc gggccgattc tgtagaatcg
     2161 cgggctatgc tggaccgtcg cgcgcaaatc ctgctcaaga ccctggtgga gcgctatatc
     2221 gccgaaggcg agccggtggg ctcgcgcgcg ctgtcgcgct actccggcct ggatctgagc
     2281 ccggccacga tccggaacgt catcgccgac ttggaggaac tcggcttcgt cgcgagcccc
     2341 cacacctccg cgggacggat tcccaccccg cgcggctacc gtttcttcgt cgacaccctg
     2401 ctcacggtca agcagctcga ccgcgtcgag atcagccagc tcgaggacaa tcttcatccg
     2461 gaccacccgc agggtctcat cagcgcggct tcgcagcttc tgtccgagct cacccacttc
     2521 gcgggcgtcg tggtagccag caaacgcaag gcaccggcct tccgtcacat cgagtttctg
     2581 gcactctctg acaagcgcgt cctgctgatc atcgtaacac cggacggtga cgtgcaaaac
     2641 cggatattgt tcacggaaaa ggcctacacg ccgcccgagc tcaccatggc ggcaaatttc
     2701 ctcaaccaga actacgccgg cctcacgttc gccgacatcc gccgccgcct gcgcgacgag
     2761 ctgaagcaac ttcgggagga tatgacgcag ctcatgaccg ccgcgctcga ggcgagcagc
     2821 gaggccttgt ccgagtcggc ggagaactat gtcatatccg gcgagaggaa tttgctcgac
     2881 tcgcacgagc ttgcgtcgaa catgtcccgg ctgcgcgaat tgttcgagct cttcgagaag
     2941 aagacgctgc tcgtgcagat tctcgagctg agcacgcacg cgcagggcgt gcagatctac
     3001 atcggcggag agtccggcgt cgcgccgctc gacgaatgca gcgtcgtcac ggctccgtac
     3061 gaaaccgatg ggcagataat cggtacggtc ggggtgatcg gaccgacgcg catggcgtat
     3121 gagcgcgtga taccgattgt cgacatcacc gcgaagctat tgtcgagcgc gctgtcgagc
     3181 cactagatcg ggagtcgaga gtccgggagc gaagcaggat cagcggctct tgttccagac
     3241 tctgactccc cgctctcggc atgcactatc tccccgagcc gaaacaggag tacggcgcca
     3301 tcgccaagac ggcggtgctg ctcgtcaatc tcggcacgcc cgatgcgccg acaccgcagg
     3361 cggtacgccg atacctgaag cagtttctgt ccgacccgag ggtgatagaa attccgcgcg
     3421 cgttttggtt gccgatcctg cacgccttcg tgctcaattc gaggccgagg aaatcggccc
     3481 ggaagtacgc gagcatctgg tccccggagg gctccccact gaaggtgcat accgagaggc
     3541 aggcgcgatt gctgcgcggc tatttcgttt cgcaggcccg catgccgctc tcggtcgatt
     3601 tcgccatgcg ctacggcgag ccctcgatcc agggcacgct ggctcgcctg aagtcggagg
     3661 gttgcgaacg cgtcctcgtg ctgccgatgt atccccaata cgcggccagc acgaccgcga
     3721 gcacgctgga tgagttagga gggtttctgc ggcgagtccg caacgtaccg gaagtccggc
     3781 tggtgaagca cttcaacgac cacccttcat acatcgccgc actggcgaat ctcgtgcgcg
     3841 agcactggcg tcaggcggga cgtccggaca cgctgctcat gagcttccac ggcttgccac
     3901 gttacaccct cgcccgaggg gacccctacc atttcgaatg tcagaagacc gcgcgcctcc
     3961 tcgccgaggg gctcgagctt gccgagagcc agtggcaaat cgccttccag tcccgctttg
     4021 gacgggcgga atggatacag ccttataccg cctccaccct ggccgactac gggcggcggg
     4081 gcatgcgccg cgtggacgtg atctgtccgg ggttcgtcgc agattgcctg gagacgctcg
     4141 aagaaatcgg catcgagggc cgaaagatct tcctcggtgc gggcggcagg gaattcaacc
     4201 tcttgccctg cctcaacgag cgcgacgact ggattcgagc cttggccgac attgcacgcc
     4261 agcatctttc cggctgggtg atggaatccg ggaatccgcg gctcgaaaag aaccgcgccg
     4321 aagacccgag ccgggccgac agctcttgaa atctccccgg agaaccccag ttagaatcgt
     4381 caactgaagg ggcacgcctg tgccccttgc cacatcgagg cacatattgc ggagacctta
     4441 tgcctgaacc ggaccagccg agcccgcagg tttcgtcggg cgatgcctca gtcgatccgg
     4501 acgcggcgat cgccaaaccg gcggatgccg cgcccgcacc cgataccgag gagctgctgc
     4561 gacaagcgga gtcgaaggcc caggaacacc acgacgcttg gctccgagcc aaggcggagg
     4621 cggaaaatat ccgcaagcgg tcggcgatcg aactcgcgaa cgcctacaag tacgcgatcg
     4681 aagccgtcgg cgcggacctt cttcccgtga aggacagtct ggaagcgacc ctcgcggccg
     4741 actccgctgc gctcgaaacg ctgaggagcg gagtcgagct tacgctgaag cagctgaacg
     4801 ccgtgttcgc gaaggccgcc ctcaaggaga tcaatcccat cggggagaag ttcgaccccc
     4861 accggcacca ggcgatcagc atgctcccct cggagaagga ggcgaacacg gtcatcaacg
     4921 tgctgcaaaa aggctacagc ctgcacgatc gggtgatccg gccggcattg gtcaccgtcg
     4981 ccaagcccaa ggacgatcga gcttgaagtt gggggattga tccccatatc ggcgttaagg
     5041 ttatttgcca gaaaattcaa aaggaaaggc catgggaaag atcattggaa tcgacctcgg
     5101 gacgaccaac tcctgcgtcg cgatcatgga gggcggcaag cccaaggtga tcgagaactc
     5161 ggaaggcgcc cgcaccacgc cctccatcgt cgcctacatg gaggacggtg aaatcctcgt
     5221 cggcgcaccg gcc
//
LOCUS       DASPZD010000342         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_360824, whole genome shotgun sequence.
ACCESSION   DASPZD010000342 DASPZD010000000
VERSION     DASPZD010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360824"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VEM33_06645"
     CDS             <1..1129
                     /locus_tag="VEM33_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51539.1"
                     /translation="TIQKQILELIAALQKRLRMSVLFITHDLALVGEIADYVVVMREG
                     EIREQGRVEQIFEAPRDAYTRALLLCRPQLTRRPRRLPVIDDFMKPGPLAGGSVEERS
                     RGLRGDEEIILEVRHLGKSFYSREGLFGKREFKAVKDVSFKVARGKTLGIVGESGSGK
                     TTVALTLLRLRGESSGEALFDGKDLLSIPQKEMMPYKRRIQIVFQNPYASLNPRFTIG
                     QLLMEPLQVHRIASSEGERRDRVYKLLEEVGLPEVSFSKYPHEFSGGQRQRIAVARCL
                     TMKPDVLICDEAVSALDVSIQAQVLNLLQDLQDEHHMSYIFISHDLAVVKYMADQVMV
                     MNEGEVVEVANSDDIYRNPQHAYTKKLLSSIPQGWRGARAR"
     gene            complement(1130..1597)
                     /locus_tag="VEM33_06650"
     CDS             complement(1130..1597)
                     /locus_tag="VEM33_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYS51540.1"
                     /translation="MRKIPLILLGALLAALAVAAPLPYDESADAKAAVRQALAAAKES
                     RIPVFVIFGANWCEDCRALDRAIQSGKNAELLAREFKVVKVDIGNFDRNLDLVAAYGN
                     PIKKGIPAAVILSPGNEVLYATRAGELADARRMSESGIYDFLRKVTQTAKAKR"
     gene            complement(1614..2000)
                     /locus_tag="VEM33_06655"
     CDS             complement(1614..2000)
                     /locus_tag="VEM33_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51541.1"
                     /translation="MAKHAILAHSVVFLAACLVASCTTTPEETIPEAEEPPLTLVKWD
                     DLPLELKKMSVPPYSAISYCAVRASSQGSDAEAVKADLAECLSADLLATAGTFLVCHL
                     AANNLQGKAYAERRRKCLIQSGAKPG"
     gene            complement(2038..2397)
                     /locus_tag="VEM33_06660"
     CDS             complement(2038..2397)
                     /locus_tag="VEM33_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51542.1"
                     /translation="MNPQLKGVEKLEGTYLFDIAASAKALRLNRFLHGFTVPGNRALF
                     KEDPEAAFDKAGLTAEERRMVRELDWAALMRYGASFFCLEKLGRVKGVSNPEMVAGFR
                     GESLEDFLKTRNVPGAR"
     gene            complement(2394..3257)
                     /locus_tag="VEM33_06665"
     CDS             complement(2394..3257)
                     /locus_tag="VEM33_06665"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HYS51543.1"
                     /translation="MAKILGGIGTSHVPTIGGAYDRNKQNDPDWAPLFRGYQPVKAWL
                     AQRKPDVLVFFYNDHATTFFFDHYPTFALGVGAEYAIADEGLGPRAVPPLKGHAGLAR
                     HMADALVNDEFDISVFQDLPIDHGVHSPLTMFWPPSPGWPGKIVPIEINVLQHPIPTP
                     ARCWKLGQAVRRAVLSYPEDLKVVIVGTGGLSHQMNGERAGFNNEKWDRKFLDLIARD
                     PKKLVAMRHADYIRLGGTEGAEEIMWLAMRGALSPRARKIHQNYYLPMTTAMAVALFE
                     EPRAKAGFKER"
BASE COUNT          620 a         1065 c         1179 g          667 t
ORIGIN      
        1 gaccatccag aagcagatcc tggagctgat cgccgcgctg cagaagcgcc tgcgcatgtc
       61 ggtgctgttc atcacccacg atctcgccct ggtgggcgag atcgccgact acgtggtggt
      121 gatgcgcgag ggcgagatca gggagcaggg gagggtcgag cagatattcg aggcgccgcg
      181 ggacgcgtac acgcgggcgc tgctgctttg ccggccgcag ctcactcggc gtcccaggag
      241 gctgcccgtg atcgacgatt tcatgaagcc cgggccgctc gccgggggaa gcgtggagga
      301 acgcagtcga gggctgcgcg gcgacgaaga gatcattctc gaggtgcgcc acttgggcaa
      361 gagcttctat tcgcgcgagg gattgttcgg aaagcgggaa ttcaaggcgg tcaaagacgt
      421 ttctttcaag gtcgcccggg ggaaaaccct gggcatagtc ggcgaatcgg gttcgggcaa
      481 gacgacggtc gcgctgacgc tgttgcgctt gcgcggcgaa tcgagcggcg aggccttgtt
      541 cgacggcaag gacctgctgt ccatcccgca gaaagagatg atgccgtaca aacgccgcat
      601 ccagatcgtc ttccagaacc cctatgcttc gctcaatccg cgtttcacga tcggccagct
      661 gctcatggag ccactccagg tgcaccgcat tgcgtccagc gaaggcgagc gccgcgacag
      721 ggtgtacaaa ttgctggagg aagtggggct ccccgaggtt tcgttctcca agtatccgca
      781 cgaattctcc ggaggccaga gacagcggat cgccgtcgcc cgctgcctca ccatgaagcc
      841 ggacgtgctc atctgcgacg aggcggtatc ggcgctcgat gtctcgatcc aggcgcaggt
      901 cctgaatctg ctgcaggacc tgcaggacga gcaccacatg agctacatct tcatctcgca
      961 cgacctcgcg gtggtcaaat acatggccga tcaggtgatg gtcatgaacg agggcgaggt
     1021 cgtcgaggtc gccaattcgg acgacatcta tcgcaacccg cagcacgcct acacgaagaa
     1081 gctgctgtcc tcgattcctc agggctggcg cggcgcgcga gcgcgctagc taccgcttcg
     1141 ccttcgccgt ttgcgtgacc ttcctgagga agtcgtaaat gccgctttcg ctcatgcgcc
     1201 gggcatcggc gagctcgccg gcacgggtgg cgtagaggac ctcgttgcct ggagagagaa
     1261 tcacggccgc gggaattccc ttcttgatgg gattgccgta tgcggcaacg aggtcgaggt
     1321 ttcggtcgaa gttcccgatg tcgaccttga ctaccttgaa ctcccgggcg agcagctccg
     1381 cattcttgcc gctctggatg gcgcggtcca gggcgcgaca gtcttcgcac cagttcgcgc
     1441 cgaagatgac gaaaacgggg atgcgactct cctttgcggc ggcgagcgcc tgtcgaacgg
     1501 ccgccttggc gtcggccgat tcgtcgtagg gaagcggcgc ggctacggcg agcgcggcga
     1561 gcagcgcgcc gagcaggatg agggggattt ttcgcatgac aggctcctaa aaatcagccg
     1621 ggtttcgccc cgctttgaat gaggcacttt ctgcgccgct cagcatacgc ctttccttgc
     1681 agattattgg cggcaaggtg gcagacgagg aaagtgccgg ctgtcgccag caggtcggcg
     1741 ctcaagcact cggccagatc cgccttcacc gcttccgcgt ccgaaccttg cgagctggcg
     1801 cgcaccgcgc agtaggagat ggcggaatag ggcggcaccg acatcttctt cagctcgagg
     1861 ggcaggtcgt cccacttcac gagagtcagc ggcggctcct cggcctccgg tattgtttct
     1921 tccggcgtcg tcgtgcaaga agcgactagg caagcggcga ggaagacgac tgaatgggca
     1981 aggattgcgt gcttggccat gctgggcgcc agtttacgct ttgagattgc cgccggtcta
     2041 gcgggcgccg ggaacgtttc gagtcttgag gaaatcctcc agcgattctc cgcggaatcc
     2101 cgcgaccatc tccggattgg atacgccttt gacgcgcccg agcttctcga ggcagaaaaa
     2161 gctcgctccg tagcgcataa gcgcggccca gtcgagctcg cgcaccatgc ggcgttcctc
     2221 ggcggtgagg ccggccttgt cgaacgccgc ttcggggtct tccttgaata gcgcgcggtt
     2281 gccgggcacg gtgaaaccgt ggaggaaacg gttgagccgc agcgccttgg cgctcgcggc
     2341 gatgtcgaag aggtaggtgc cttcgagctt ttcaacgccc ttgagctggg gattcatctc
     2401 tctttgaagc cggcttttgc tcgcggctcc tcgaacagcg ccaccgccat cgcggtggtc
     2461 atcggcaggt agtagttctg gtggattttc ctcgcgcgcg gcgagagcgc gccgcgcatc
     2521 gcgagccaca tgatctcctc cgccccttcg gtgccgccca gcctgatgta gtcggcgtgg
     2581 cgcatcgcca cgagtttctt cgggtcgcgc gcgatcaggt cgaggaattt cctgtcccat
     2641 ttctcgttgt tgaagccggc gcgctccccg ttcatctggt gcgaaagtcc tccggtgccg
     2701 acgatgacca ccttcaggtc ctcggggtag gagagcacgg cgcggcgcac cgcctggccg
     2761 agcttccaac agcgcgcggg ggtggggatc gggtgctgga gcacgttgat ctcgatcggc
     2821 acgatcttcc cgggccagcc gggcgagggc ggccagaaca tggtgagggg cgagtgcacg
     2881 ccgtggtcga tcggcaggtc ctggaaaacg gagatgtcga actcgtcgtt caccagcgcg
     2941 tcggccatgt ggcgcgcgag gccggcgtgg cctttcaaag gcggaaccgc gcgcggtccc
     3001 aggccctcgt cggcgatcgc gtactcggcg cccacgccca acgcgaaagt cgggtagtga
     3061 tcgaagaaaa acgtggtcgc gtgatcgttg tagaagaaca cgagcacgtc gggcttcctc
     3121 tgcgcaagcc aggccttgac cggctgatag cccctgaaga gcggagccca gtcggggtcg
     3181 ttctgcttgt tgcggtcgta ggcgccgccg atcgtgggga cgtgcgaggt gccgatgccg
     3241 ccgaggatct ttgccatgga tcaaattccg ggggaatgat gagccgggta cattgtcacg
     3301 ttccgcgcac gaattatcgc ctgtccgggc tggcatagcg ctttcctgac agtaggtgtc
     3361 gggtttcccc gatggcggca cgcacccgcg aggcatatcg ttgatcggac tcgcctactt
     3421 ccaaaaaaaa ggaactccaa tgccgaaatt tgctggtgtc atcggggccg ggaccctggc
     3481 ccttttcgtc gcgagcgggg cctcggcgca agccgaacgc ctgaccctgg g
//
LOCUS       DASPZD010000343         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1221945, whole genome shotgun sequence.
ACCESSION   DASPZD010000343 DASPZD010000000
VERSION     DASPZD010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221945"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VEM33_06670"
     CDS             complement(<1..1214)
                     /locus_tag="VEM33_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HYS51544.1"
                     /translation="MQVTRRGSIGFALALGLAAASGASQAALSCANLASVTPEASNIT
                     SATMVTAPTTIGGAAVSVPFCRVQGTARPSLDSEIKFEVWLPPTAADWTGRMKVNGTG
                     GYAGATPYARLAQDIGDGFITAGSNMGHDGGEAASWTMGHPEKVKDWGLRAHYYVATA
                     AKTLSVAFYDRPVAHSYFEGCSNGGRQAMMMAQNYPELFDGIVAGAPSMFYPDILFWL
                     LWTGKQLTPVFGQPAALSTTKRNLITDRVLQACDANDGLVDGQITNPRACSFDIDRLG
                     PSGENVLTAQELATVKAMYAGTTNEITGQQRYSGAKFGSEANFDPNFADNGGYGPFIG
                     HFVYSLLSPPYEWRRDINWSTVYDQAKAVLSPITAAPSPDIRAFTRRGGKIVHYHGWN
                     DSVVPPDGSIAYL"
     gene            1390..2034
                     /locus_tag="VEM33_06675"
     CDS             1390..2034
                     /locus_tag="VEM33_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HYS51545.1"
                     /translation="MRVGILGSGLMGAKLGTIFARAGHEVVFSYARSERKLKRLAREA
                     GGKARAGTPREAAQDADAVLLAVHWLRFDDVLKQAGDLSGRIVVTCSMPMNEDNTELV
                     VAHTSSGAEELAKRIPKARVVAAFGTVPSEVFFDVYEARRKAKRPSLVYCGDDRRGKE
                     AAAGLIRDVGFDPVDAGPLRIARYTEPFTMLIAQLAYEGAGGPELAYRFERFKK"
     gene            2043..2585
                     /locus_tag="VEM33_06680"
     CDS             2043..2585
                     /locus_tag="VEM33_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51546.1"
                     /translation="MPELAEWESFYVIVGGGAAALIGLQFVVLALIAEKPQLNAPEAG
                     AAFATPTIVHFSTVLLIAAVLRVPWQGVAAASTLWGLVGLSGAAYTVLVARRVRRQRS
                     YRPDLEDWLFHSLFPLAAYAVLALSSLAAHAHAHESLIAVGAATLLLLFIGIHNAWDA
                     VSYFVFVRMRNAKDVESPKE"
BASE COUNT          440 a          865 c          897 g          503 t
ORIGIN      
        1 aggtaggcga tcgatccatc cggcggcact accgagtcgt tccagccatg gtagtggacg
       61 atcttgccgc cgcgtctcgt aaacgcccgg atgtccgggc tgggtgccgc ggtgatgggc
      121 gagagcaccg ccttcgcctg atcgtagacc gtcgaccagt tgatgtcgcg gcgccattcg
      181 tagggcggtg acaacagcga atacacgaag tggccgatga acgggccgta gcctccgttg
      241 tcggcgaagt tcggatcgaa gttcgcctcc gagccgaact tggcaccgct gtagcgctgc
      301 tggccagtga tctcgttcgt cgtgccggcg tacatcgctt tgacggttgc gagctcctgc
      361 gcggtgagga cgttttcgcc ggaagggccg agcctgtcga tgtcgaagct gcaggcgcgc
      421 gggttcgtga tctgtccgtc gacgagaccg tcgttcgcgt cgcaggcctg gagcacgcga
      481 tccgtgatca agttacgttt cgtggtgctc agggcggccg gctgtccaaa gacgggcgtc
      541 agctgcttgc ccgtccagag cagccagaac aggatgtcgg ggtagaacat cgagggcgct
      601 ccggcaacga tgccgtcgaa gagctcagga tagttctgcg ccatcatcat cgcctggcgc
      661 ccgccgttcg agcagccttc gaagtaggaa tgggcgaccg gcctgtcgta gaaagcgacg
      721 ctcagcgtct tcgccgcagt ggccacgtag tagtgcgccc gcaggcccca gtccttcact
      781 ttttccgggt ggcccatcgt ccagctcgcc gcctcgccgc cgtcatgtcc catgttgctg
      841 ccggcggtaa tgaagccgtc gccgatgtcc tgtgccaagc gcgcgtaagg cgtggcgccg
      901 gcatagccgc cggtgccgtt caccttcatg cgtccggtcc agtcagcggc ggtgggtggc
      961 agccacacct cgaacttgat ctccgagtcg agcgacggcc tggcagtacc ctgcacacgg
     1021 cagaacggca cgctcacggc ggcgccgccg atcgtcgtcg gcgcagtcac catcgtcgcc
     1081 gacgtgatgt tggatgcctc cggagtcacc gatgctaaat tggcgcagct caaggcggcc
     1141 tggcttgccc cggacgcggc cgccagcccg agcgcgagcg cgaacccgat gctgcccctc
     1201 ctcgtcactt gcatgattcc ccctatgatt ggttgaatgg gcccactccg ccggcgcccc
     1261 gcgtgcaaga tagcagtttc atcgctgccg atcgatttga gatactgatc aatcgatgtg
     1321 catctctcat gaatacgagg ctacggccat ttgccggttt ggattgactt ctacccttag
     1381 gagataacca tgcgagtcgg cattctcggt tcaggcttga tgggcgccaa gctcggaaca
     1441 atcttcgctc gagcgggaca cgaagtggtc ttcagctatg cccgcagcga gcggaagctc
     1501 aagcggcttg cacgcgaggc cgggggcaag gcgcgggccg gcacaccgcg cgaggcggcg
     1561 caggacgcgg atgcggtatt gctggccgtg cactggctgc ggttcgacga tgttctgaaa
     1621 caggcgggcg acctgtcggg aaggatcgtc gtgacctgct cgatgccgat gaacgaggac
     1681 aataccgagc tcgtcgtggc ccatacctcg tcgggcgcgg aggagcttgc gaaaaggatt
     1741 cccaaagctc gcgtcgtggc ggcattcggg accgtcccga gcgaagtgtt cttcgacgtc
     1801 tatgaagcga ggcgcaaggc gaagcggccg agcctcgtgt actgcggcga cgaccggcgc
     1861 ggcaaggaag cggcagccgg cctgatccgc gacgtcggct tcgatccggt ggacgccggg
     1921 cctttgcgga tcgcccgcta taccgagccg ttcacgatgc tcatcgcaca gctcgcttac
     1981 gaaggcgcgg gaggcccgga actggcgtat cgtttcgagc ggttcaaaaa ataggagacg
     2041 acatgcccga gcttgccgaa tgggaaagct tctacgtcat cgtcggcggg ggcgcagctg
     2101 ccctgatcgg cctgcagttc gtcgtcctgg cgctaatcgc agaaaaaccg caactgaacg
     2161 cgccggaggc cggcgccgcg ttcgctacgc cgacgatcgt ccatttcagc accgtgttgc
     2221 tcatcgcggc ggttctgcgc gttccctggc aaggcgtcgc cgccgcgtct acgctctggg
     2281 ggctcgtggg cttgagcggc gcggcgtaca ccgtcctcgt ggcgcggcgc gtccggcggc
     2341 agaggtcgta ccggcccgat ctcgaggact ggctgtttca ttccctgttc ccgttggccg
     2401 cgtacgcggt gctcgcgctc tcgtcgctcg ccgcgcacgc tcacgcccac gaatccttga
     2461 tcgccgtcgg cgcggcgacc ctgctgctgc tcttcatcgg catccacaac gcctgggacg
     2521 ccgtttccta cttcgtgttc gtgaggatgc ggaacgcgaa ggacgtggaa tcgccgaagg
     2581 agtgaattca gccgagcgct tcgcgcgcgg cgcgcacgcc gcttctcagg gcggcggcga
     2641 cggtgccggc ttcttccctc tcggtggcct cgccggcgaa gaacaccgtc ccgccgagcg
     2701 gcacc
//
LOCUS       DASPZD010000344         8572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1363671, whole genome shotgun sequence.
ACCESSION   DASPZD010000344 DASPZD010000000
VERSION     DASPZD010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363671"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VEM33_06685"
     CDS             complement(<1..181)
                     /locus_tag="VEM33_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYS51547.1"
                     /translation="MKRNQAPLPGKVAALLRESRLFVLVAGALYLSLVLTTFNRADPG
                     WSHSVAAGEIRNLGGR"
     gene            complement(198..872)
                     /locus_tag="VEM33_06690"
     CDS             complement(198..872)
                     /locus_tag="VEM33_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYS51548.1"
                     /translation="MTAPASVSMLLLRNVPLFSTLPEHQLALLTGVVSRKSFPRGASI
                     IAAGDVTESLYVVISGRMKVMMSDDEGREVILAILGPNEFFGEMGLIDDHPRSASVVA
                     LEACEVLSLSKRDFKKSLADNFEMAMTVMRGLVKRLREADQKIGSLALMDVYGRVARL
                     LLEMAETVNGQKVVTRKLAKQDIAKMIGASREMVSRVMKDLQSGGYIEVRPGSIFLHE
                     TILTID"
     gene            1072..2019
                     /gene="trxB"
                     /locus_tag="VEM33_06695"
     CDS             1072..2019
                     /gene="trxB"
                     /locus_tag="VEM33_06695"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HYS51549.1"
                     /translation="MSKKTRHSRLLILGSGPAGYTAAVYAARANLKPVVITGLAPGGQ
                     LMTTTDVDNWPADVKGAQGPELMERFQKHAERFETEVVFDHIHTVNLRERPLRLQGDA
                     GEYTCDALIVATGASARYLGLESEKKFMGKGVSACATCDGFFYKGQDVAVVGGGNTAV
                     EEALYLSNITRHVTVIHRRDKFRAEKILQKKLFDRGNVTMRWNHVLDEVLGDAKGVAG
                     IRIKDVQTGATQQIDLQGLFIAVGHTPNTGIFEGQLDMKGGYIVTRGGSEGNASATSI
                     EGVFAAGDVQDHVYRQAVTSAGSGCIAALDAEKYLDGLR"
     gene            2062..2613
                     /locus_tag="VEM33_06700"
     CDS             2062..2613
                     /locus_tag="VEM33_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HYS51550.1"
                     /translation="MRKRTSSGLSEHEAALFRDAVRGATPLESSARVEPKAKAPPPVP
                     VQSLLDGHDALVESLAGPISWDQSMETGNELSYLRSGLARDVLRKLRRGHWVVQDVVD
                     LHGLNRQEARSSLAEFLAACLKRGLRCVRVVHGKGLRSPGREPVLKGKVQQWLAQRNE
                     VLAFCEAPKNQGGGGALLLLLKA"
     gene            complement(2680..3018)
                     /locus_tag="VEM33_06705"
     CDS             complement(2680..3018)
                     /locus_tag="VEM33_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYS51551.1"
                     /translation="MKKIEAVVKPFKLDEVREALAEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVTEKMLESAIEAIVKAARTGKIGDGKIFVSTVEQVVRI
                     RTGETNEAAV"
     gene            complement(3103..4794)
                     /locus_tag="VEM33_06710"
     CDS             complement(3103..4794)
                     /locus_tag="VEM33_06710"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HYS51552.1"
                     /translation="MASIVKIAIAQINCVVGDLAGNANKILGYCERARAAGADVILTP
                     ELALCGYPPEDLLLRGGFYRDCNAALERLAREVSGITLVVGHPHEDRGKRHNAASVVK
                     DRRVVETYYKHVLPNYTVFDEERYFVPGDFPCVFQVGETRFGVNICEDTWGPQGPLTT
                     KPDAVRIGINICADAWEAKAPQAAREAGAQVLLVLNASPYHMRKQESRYDVMRERVIE
                     TGMALVYANMVGGQDELVFDGASFALDRRGEVVCQLPVFEEALEFVTVADGDPQRGGI
                     AEQASLESEVYKALCLGVRDYVGKNGFPGALVGLSGGVDSALTLCIACDALGPERVRA
                     VMMPSQYTSRMSLEDSRELAKNLRVRYDEIPIGPAFDAFLGLLAKEFAGLPADATEEN
                     LQARIRGTLLMALSNKFGAIVLTTGNKSEMAVGYATLYGDMAGGFAVIKDILKTLVYR
                     LCRHRNGMDRVIPERVLTRAPSAELKANQTDQDSLPPYDVLDAIISAYMENDLSPAEM
                     VERGFRTEDVRRVVEMIHRSEYKRRQAPIGIRITHRGFGKDWRYPITSRYREPYE"
     gene            4890..6020
                     /locus_tag="VEM33_06715"
     CDS             4890..6020
                     /locus_tag="VEM33_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYS51553.1"
                     /translation="MKEDTPSVKVINTLRAVEPRAWNALAAGHPFLRHEFLHALHETG
                     CACESTGWSPCYLTLWQGASLVGAMPLYSKSHSYGEYVFDWAWADAYHRHGLSYYPKL
                     LAAIPFSPVTGPRLLARDAETRALLVRAALRLSLEVSSLHVLFPAGPEAREMEAAGMM
                     LRRSVQFHWENQGYADFDEFLSGLASAKRKKIRQERRRVQEAGVRVRRLVGAEIRDEN
                     WSFFTRCYNNTYRAHHSTPYLNLEFFRRLGETLPEHLLMVLAELDGRPIASALNIFSP
                     EVLYGRYWGSVTHVPLLHFEVCYYQALDFCIERGIRIFEGGAQGEHKLARGFMPNPTW
                     SAHWLRHPEFSDAVEKFLARESQGIERYVDELNERSPFRAKN"
     gene            complement(6027..7430)
                     /locus_tag="VEM33_06720"
     CDS             complement(6027..7430)
                     /locus_tag="VEM33_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYS51554.1"
                     /translation="MPSFADLNLLPELLKAVAEQGYVEPTPIQAQAIPLVLQGRDVLG
                     GAQTGTGKTAGFALPLLQRLARHANTSASPARHPVRALILTPTRELAAQVEESVRTYG
                     KYVGLRSTVVFGGIDMKPQTAALRQGVEFLVATPGRLLDHVQQKTVNLQQVEAFVLDE
                     ADRMLDMGFIPDVRRIMALLPKQRQNLLFSATLSQDIRTLADSFLHDPVLVQVARLNT
                     AAELVQHVAHPVRRERKRELLAYLVQSRDLRQVLVFTSTRIGANRLAYQLNRDGIHTS
                     AIHGDKSQPERLQALADFKSGKVRVLVATDVAARGLDIEDLPHVVNFELPGVPEDYVH
                     RIGRTGRAGASGEAISLVCAEEHERLDAIEKTIRLSIARKIVPGFEPDLSVAPSMMSR
                     PTGRRDPAARERRPRQAGRERDPLTRAPQTQKTLDPIFSSPYKPALATSAATGPAPGR
                     QAPRQVAALLCGPPKNK"
     gene            complement(7501..8559)
                     /gene="queG"
                     /locus_tag="VEM33_06725"
     CDS             complement(7501..8559)
                     /gene="queG"
                     /locus_tag="VEM33_06725"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HYS51555.1"
                     /translation="MRPADLAMLAATIKSWGRELGFQAVGIADTDLSAVEPRLAAWLA
                     SGFAGELDYMRKHGAKRTRPASLVPGTLRIISARMDYRPDAADSQGILEDGSKAFVAR
                     YALGRDYHKVLRARLRRLAEKIAGEIGEFGYRVFTDSAPVMEVELARKAGLGWRGKHT
                     LLIAREAGSFFFLGDIYTDLPLPLDAPVDEHCGSCTKCLDICPTHAIVAPYVVDARRC
                     ISYLTIELKGSIPLELRPLIGNRVFGCDDCQLVCPWNRYAQLAPEADFQVRNGLDCAE
                     LVDLFAWTEEEFRQRMSGSAILRIGYERWLRNLAVGLGNAPKSSRAVAALRSRVRESS
                     ALVREHVAWALERQGAGP"
BASE COUNT         1567 a         2767 c         2671 g         1567 t
ORIGIN      
        1 cccgcccgcc gaggttgcgg atctcgccgg ccgctacgct gtgggaccac cccgggtctg
       61 cccggttaaa cgtggtgagg acgagggaga gatacaaggc gcccgcgacc aggacgaaca
      121 gcctcgactc gcgcagcagc gcggcgacct tgccgggaag gggcgcttgg ttccgtttca
      181 aagcctagcc cgacccctca atcgatcgtc agtatggtct cgtgcaggaa aatcgaaccg
      241 gggcgcacct cgatgtagcc gcccgactgg aggtccttca tcacgcggct caccatctcg
      301 cgcgaggctc cgatcatctt cgcgatgtcc tgcttggcga gctttcttgt gacgactttc
      361 tggccgttca cggtttccgc catttcgagc agcaagcgcg ccactctgcc gtacacgtcc
      421 atcagggcga ggctgccgat cttctggtcg gcttcgcgaa gccgtttgac cagtccccgc
      481 atgaccgtca tggccatctc gaaattgtcg gcgaggcttt tcttgaaatc gcgcttcgag
      541 agactgagca cctcgcaagc ctccagggca accacgcttg ccgagcgcgg atgatcatcg
      601 atcaggccca tctccccgaa aaactcgttg ggtccgagga tcgcaaggat cacttcgcgc
      661 ccctcgtcat cgctcatcat gaccttcatg cgacccgata tcaccacgta aagggactcg
      721 gttacatccc ctgcggcaat gatggaagct ccgcggggga agctcttgcg gctcaccaca
      781 ccggtgagga gcgccagctg atgctcgggc aacgtcgaaa acagcgggac gttgcgcaga
      841 agcagcatcg atacgctagc gggggccgtc atcgcctcta cctcccaggg aacctggttc
      901 tacccgcata tcttagcaga ctcgcgaaga ccgcttcatg ctgcgagtcc ggtcgaaatc
      961 gcagcaattc agcaggccgc agccggcgga gcagcgattc agcggggtta taatttggaa
     1021 ccgcacgacg attgaattga attcgcgcat cttagtcccc accattccct catgtcgaag
     1081 aaaaccaggc actcgcggtt gttgatactg gggtcgggcc cggcgggcta caccgccgcc
     1141 gtctacgccg cgcgcgcaaa cctgaaaccc gtcgtcatca ccggcctcgc tcccggcgga
     1201 cagctgatga cgacgaccga cgtggacaac tggcccgctg acgtgaaggg agcgcaaggt
     1261 ccggagctga tggaacgttt ccagaagcac gccgaacgtt tcgaaactga agtcgtgttc
     1321 gatcatatcc acactgtaaa tctccgggag cgacctctcc ggctgcaggg cgacgcgggt
     1381 gaatacactt gcgatgcgct catcgtcgcc accggagcct ccgcccgcta tctcgggctc
     1441 gaatccgaga agaagttcat gggaaagggc gtctccgcct gtgcgacctg cgacggattc
     1501 ttctacaagg gtcaggacgt cgccgtcgtc ggcggcggga acaccgcggt ggaagaagcg
     1561 ctctacctct ccaacattac gcgtcacgtc accgtgatcc accgacggga caaattccgg
     1621 gccgagaaga tccttcagaa gaagctcttc gacagaggta acgtcacgat gcgctggaac
     1681 catgttttgg acgaggtcct cggcgatgca aagggggtag ccggcatccg catcaaggac
     1741 gtccagaccg gcgcgacgca gcagatcgat ctgcaggggc tgtttatcgc agtgggccac
     1801 accccgaata ccggcatttt cgagggacag ctcgacatga aaggcggata catcgtcacc
     1861 cgcggcggga gcgagggcaa cgccagcgca accagcatcg agggcgtgtt cgctgcgggc
     1921 gacgtgcagg accacgtcta ccggcaggcc gtgacgagcg cgggcagcgg ttgcatcgca
     1981 gccctcgatg cagaaaaata tctggacggc ctgcgctaaa gtcccgcaac aaggctcgga
     2041 tgcagtgccg aggacgaaat catgcgcaag cgcacgagta gcgggctttc cgaacacgaa
     2101 gccgccctgt ttcgcgacgc agtgcgcggt gcaacgcccc tcgagtcttc ggcccgggtc
     2161 gagcccaagg ccaaggcgcc gccgcccgtg ccggttcaat cgttgctcga cgggcatgac
     2221 gcgctcgtcg agagtctcgc cggtcccatt tcctgggatc aatcgatgga gaccgggaac
     2281 gagctctcct atctgcggag cggtctagcc cgcgatgtgc tgcgcaagct gagacgaggg
     2341 cattgggtcg tgcaggacgt cgtcgatttg catggcctga accggcagga ggcccggtcg
     2401 tcgctggcgg agtttctcgc cgcgtgcctc aagcggggat tgcgctgcgt tcgcgtggtc
     2461 cacggaaagg gtctgcgctc gcccggacga gagccggtgt tgaagggaaa agtgcagcag
     2521 tggttggcac agcgaaacga agtcctcgcc ttctgcgagg ccccgaaaaa tcaaggtggg
     2581 ggcggtgcgt tgctgctgct gctgaaagcg taggctctag gcaagtcgag caggctttct
     2641 tccctggatc cttcgacccg ctgagttccc acgccgcggc tagacggccg cctcgttggt
     2701 ttcgcccgtc cggatacgca ccacctgctc cacagtgctc acgaaaatct tcccgtcgcc
     2761 gatcttgccg gtacgcgccg ccttgacgat ggcctcgatc gcgctctcga gcatcttttc
     2821 cgtcaccacg acctcgatct tgaccttcgg caggaagtcc acgacatatt cggcgccgcg
     2881 atagagttca gtgtggccct tctggcgacc gaagcctttc acctccgtca ccgtcagccc
     2941 agtgactccg acttcggcga gggcctcgcg aacttcgtcg agcttgaaag gcttcactac
     3001 ggcttcgatt ttcttcatgg acgtgctccc gcgttgactt ctgcatgcca gtataacaaa
     3061 cgggagtcga agcttgcctg atgaggctct cgccatcagc ggctactcgt acggctcccg
     3121 gtagcgcgat gtgatcggat agcgccaatc cttgccgaaa ccgcggtggg tgatccggat
     3181 cccgatcggt gcctggcggc gcttgtactc gctgcggtgg atcatctcga caacacgcct
     3241 cacgtcttcg gtcctgaatc cgcgctccac catttcggcc gggctcaagt cgttttccat
     3301 ataggccgag atgatggcat ccaagacatc atagggagga aggctgtcct ggtcggtttg
     3361 attggccttg agctcggcag aaggcgcgcg ggtcagcacg cgttccggaa tgacgcggtc
     3421 catgccgttc cgatgcctgc acaggcggta caccagcgtc ttgagaatgt ccttgatcac
     3481 cgcaaagcca ccggccatgt ccccgtagag ggtcgcgtag ccgaccgcca tctccgactt
     3541 gttgccggtc gtcagcacga tcgctccgaa tttgttcgac aaggccatca gcaaggtgcc
     3601 acggatccgc gcctggagat tctcctctgt tgcgtccgcg ggaaggcccg caaactcctt
     3661 tgccagcaaa ccgagaaagg cgtcgaacgc cgggccgatg gggatctcgt cgtagcgcac
     3721 cctgagattc ttggcgagtt cgcgcgagtc ttcgaggctc attcgggagg tatactgcga
     3781 aggcatcatg accgcgcgta cccgctccgg cccgagcgcg tcgcaagcga tgcatagcgt
     3841 caacgccgag tcgaccccgc cggaaaggcc gaccagcgcc cctggaaatc cattcttccc
     3901 gacgtagtcg cgtaccccga ggcaaagcgc cttgtagacc tccgactcga gtgaagcctg
     3961 ctctgcgatt ccgcctcgct gcggatcgcc atcggccacc gtcacgaatt ccagcgcctc
     4021 ctcgaacacc gggagctggc ataccacctc ccctcgccgg tcgagcgcaa acgaggcgcc
     4081 atcgaacacc agctcgtcct gccctcccac catgttcgcg taaacgagcg ccatgcccgt
     4141 ctcgatcacg cgttcccgca tcacgtcgta gcgggactct tgcttgcgca tgtgatacgg
     4201 cgaggcgttg agcacgagca gaacctgcgc gcccgcctcg cgtgccgcct gtggcgcctt
     4261 cgcctcccag gcatccgcgc agatgttgat tccgatgcgg acggcatccg gcttggtcgt
     4321 cagcggcccc tgcggccccc acgtgtcttc gcagatgttc acgccgaagc gggtctctcc
     4381 aacttgaaag acgcacggaa agtcgccggg cacgaagtag cgttcctcgt cgaacacggt
     4441 gtagttcggc aggacgtgct tgtaataggt ctccacgacc cgccggtctt tcacgacgga
     4501 tgcggcgttg tggcgtttcc cccggtcttc gtgcgggtgc cctacgacga gcgtgattcc
     4561 gctcacttcg cgcgccaggc gctcgagcgc ggcgttgcag tcgcgataga aacctccgcg
     4621 cagcagaagg tcttcgggcg ggtacccgca cagggcgagc tccggggtca gtatcacgtc
     4681 cgcgcctgcg gcgcgcgcac gttcgcagta tccgaggatc ttgttggcgt ttcccgcaag
     4741 gtcgccgacg acacagttga tctgggcaat cgcgatcttg acgatgctag ccatgcggca
     4801 agtttaccat cggcggatga gcccccgcgc ccgacggttc aatcgattct ataatcggca
     4861 gccgtgagcg ccacttcgga tcgggtcgat tgaaggaaga cacgccctcg gtcaaggtta
     4921 tcaatacgct ccgcgccgtc gagccgcgag catggaatgc gctcgcggca ggacacccct
     4981 tcttgcggca cgaatttctg catgcgctcc atgagacggg gtgtgcctgc gagagcacgg
     5041 gctggtcgcc ttgctatctg acgctgtggc agggcgcatc gctcgtcggt gccatgcccc
     5101 tctactccaa gtcgcattcc tacggcgagt acgtcttcga ctgggcatgg gccgacgcct
     5161 atcaccgtca tggtctctcg tactatccca aactgctcgc ggcgatcccg ttttcgccgg
     5221 tcaccggccc ccgtctgctc gcgcgcgacg cggagacccg ggccctcctc gttcgtgccg
     5281 cattgcggct ctcgctggag gtttcgtccc tgcatgtgct gtttccggcc gggcccgaag
     5341 cacgcgagat ggaggccgcg ggaatgatgc tgcgccgctc ggtccagttt cattgggaaa
     5401 accaaggcta cgcggatttc gacgagtttc tctccggtct cgcttccgcg aaacgcaaga
     5461 agatccgaca ggagagacga cgggtgcagg aagcgggcgt gcgagtgcga cggctggtcg
     5521 gagccgaaat ccgcgacgag aactggagct ttttcacgcg ctgctacaac aacacgtatc
     5581 gggcgcatca ctcgacgccg tatctgaacc tggagttctt ccggcgcctc ggcgagacgc
     5641 ttcccgagca cctcctcatg gtgctcgccg agctcgatgg aaggccgatc gcgagcgccc
     5701 tcaatatctt ttcgccggaa gtgctctacg ggcgttactg gggctcggtc acgcatgtcc
     5761 cgctgctgca tttcgaggtc tgctattacc aagccctgga tttctgcata gaacgcggga
     5821 tccggatctt cgaaggaggc gcgcaaggcg aacacaagct cgcgcgaggc ttcatgccga
     5881 accccacgtg gtccgcgcac tggctgcgcc atccggaatt ctcggacgcg gtggagaaat
     5941 ttcttgccag ggaatcgcag ggaatcgagc gttacgtgga cgagctcaac gaacgaagcc
     6001 cgttccgggc aaaaaactag aagcgcctac ttgttcttgg gcgggccgca gagcagcgcg
     6061 gcgacttggc gcggtgcctg ccggccaggc gccggcccgg tcgcagccga tgtcgcaaga
     6121 gccggtttgt agggagagct gaaaatcggg tcgagagtct tctgggtctg aggtgcccgg
     6181 gtcaatgggt cgcgctctcg accggcttgg cgcgggcggc gctcccgcgc cgccgggtca
     6241 cgtcggccgg ttggcctgct catcatcgag ggcgcgacac tcaggtcggg ctcgaatccc
     6301 ggaacgatct tcctcgcaat gctgaggcgg atggtcttct cgatcgcatc gagccgttcg
     6361 tgctcctcgg cacaaaccag cgagatcgcc tcgccgctcg ccccagcccg gccggtgcga
     6421 ccgatgcgat ggacgtagtc ttccggcaca ccggggagct cgaaattcac gacgtggggc
     6481 aagtcttcga tatcaagccc gcgcgcggcg acgtcggtcg ccaccagcac gcgaaccttt
     6541 cccgatttga aatcggcgag ggcctgaagg cgctcaggct ggctcttgtc gccgtggatg
     6601 gcggaggtat gaatgccgtc gcggttgagc tggtatgcga ggcggttggc gccgatgcgg
     6661 gtcgaagtga agaccagcac ctggcgcaaa tcgcgcgact gcaccagata cgcgagcagc
     6721 tcgcgcttgc gttcgcgccg gaccgggtgc gccacgtgct gcaccaactc ggctgcggtg
     6781 ttgagccgcg ccacctgaac cagcaccgga tcgtgcagga atgagtcggc gagcgtgcga
     6841 atgtcttgcg acagcgtggc cgaaaacaac aggttctggc gctgcttggg cagaagcgcc
     6901 atgatgcgac gcacgtccgg gatgaatccc atgtcgagca tgcggtcggc ttcgtcgagc
     6961 acaaacgctt cgacttgctg cagattcacc gttttctgct gaacgtggtc gagcagccgg
     7021 ccgggagtcg ccaccagaaa ttccacgccc tgccgcagcg ccgccgtctg cggtttcatg
     7081 tcgatccccc cgaacaccac ggtgctgcgc agaccgacgt actttccata cgtgcgcaca
     7141 ctctcctcca cctgggccgc cagctcccgc gtcggggtaa gaatgagcgc gcgcaccgga
     7201 tggcgcgcgg gagatgcgct ggtattggcg tgccgcgcga gccgctgcag gagcggcaag
     7261 gcgaatccgg cggtcttgcc ggtgccggtc tgggcacccc ctaggacgtc tcggccctgc
     7321 agcaccaagg gaatcgcctg cgcctggatc ggcgtaggct cgacgtagcc ctgctccgcg
     7381 accgctttca gcagctcggg cagcagattc agatcagcaa aactgggcaa acgagaactc
     7441 taatgggggt ggacctgcgg acaaggcgga tgacaagcgc gcccattata ccaaacggca
     7501 tcaggggccc gctccctgac gttccagcgc ccacgcgacg tgctctcgaa cgagggcaga
     7561 cgattcccgc acccgggacc gcagggcggc gaccgcacgc gagcttttcg gggcgttgcc
     7621 gagtcccacg gcgaggttgc gcagccagcg ttcatagccg attctgagga tggcgctgcc
     7681 actcatccgc tgccggaatt cctcctcggt ccacgcgaaa aggtcgacca gctcggcgca
     7741 atcgagcccg ttgcgcacct ggaaatccgc ctccggcgcg agttgcgcgt agcgattcca
     7801 cgggcaaacg agctggcaat cgtcgcagcc gaagacacgg ttgccgatga gggggcgcag
     7861 ctcgagcgga atgctccctt tcaactcgat cgtgagatac gaaatgcacc gccgggcgtc
     7921 gacgacgtag ggagcgacga tcgcgtgcgt ggggcagata tccaggcact tggtgcagct
     7981 gccgcaatgc tcgtccaccg gagcgtccaa tggaaggggc agatccgtgt agatgtcccc
     8041 gagaaagaag aacgaacctg cttcgcgcgc gatcagcagc gtgtgcttgc cccgccagcc
     8101 gaggcccgct ttgcgcgcaa gctccacttc catcaccggc gccgaatcgg tgaatacgcg
     8161 gtaaccgaat tcaccgattt cgcccgcaat cttttccgcg agccgccgca gccgcgcccg
     8221 cagcaccttg tgatagtcgc gcccgagcgc gtaacgcgcg acgaaggcct tgctcccgtc
     8281 ctcgagaatc ccctggctgt ccgccgcatc gggtcgatag tccatgcgcg cggaaatgat
     8341 gcgcaaggtt ccgggaacga gcgacgccgg gcgcgtcctt ttcgcgccgt gctttcgcat
     8401 ataatcgagt tcgccggcga atcccgatgc gagccatgca gcgaggcggg gctcgaccgc
     8461 cgagaggtcg gtgtcggcga ttccgaccgc ctggaagccc agttcccgtc cccaggattt
     8521 gatcgtcgcg gcgagcatcg caagatcggc gggccgcatg cgtcgcgccc gg
//
LOCUS       DASPZD010000345         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_268442, whole genome shotgun sequence.
ACCESSION   DASPZD010000345 DASPZD010000000
VERSION     DASPZD010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268442"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VEM33_06730"
     CDS             <1..919
                     /locus_tag="VEM33_06730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYS51556.1"
                     /translation="PDGRLKLADFGIARIDRSNLTQVGSIMGTPAYMSPEQYAGQQVD
                     RRSDIFSCGVVLYELLTGVKPFEGPTETVGYKICHEPHRNPSEINPQGVPEVFDAILG
                     KALAKKPEDRYAKAREFAAALAKAFESRGGAAVLTEATILPTIIHQDRRDTTSPPPGW
                     EPGQLHALEELLAPYVGPMARVLVKRSAKTTTDGPTLVRLLAASVSERDGKAFAAAAL
                     EKVFAIGQPEGAAERSSPGIWNKAVEAADVDKAAGQLAPYVGPIAKVMVKKAAAQTRD
                     LKAFYERLADNLSDPDERAEFLKNSGYGE"
     gene            complement(926..1996)
                     /locus_tag="VEM33_06735"
     CDS             complement(926..1996)
                     /locus_tag="VEM33_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS51557.1"
                     /translation="MSTSKNAPGGRPGPSCACCPPRRDFLGKLAALGASAMLPGELMA
                     QAGAKAHRIDVHHHLMYPGYLEEVGGRRAGSTFKWSPAMSIEDMDKSGIAVSVLSLIQ
                     PGAVTNEVEKGRKIARLSNEYGAQLARDHKGRFASFATLPLLDTEGALKEIGYALDTL
                     KAEGIGLMTSYQDKYLGDASFAPIWEELNRRKAVIYTHPLSPDCCRNVKTEVPPAVVE
                     YATDTTRTIASLVFSGTAARYPDMRWIFSHSGGTMPFLLSRFTRHELDMKEKAKECVP
                     KGVMYELKKFHYDTAQGNHAGALAALMKIVTPSQVLYGTDFPFRDGAEEIEGLAAFRF
                     NPGDLRAIERENALKLLPTLKA"
     gene            2076..>2269
                     /locus_tag="VEM33_06740"
     CDS             2076..>2269
                     /locus_tag="VEM33_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51558.1"
                     /translation="MNSLQYAWLAGLLLAASAWAQDPAPGYPGRPIRVIVPFPAGGAA
                     DALPRIVGEKLAARWGQPVVV"
BASE COUNT          384 a          727 c          750 g          408 t
ORIGIN      
        1 gcctgacggc cgtctcaagc ttgcagactt cgggattgcc cggatcgacc gctccaacct
       61 cacccaggtc gggtccatca tgggcacgcc tgcgtacatg tcaccggagc aatatgcagg
      121 gcagcaggtc gatcggcgca gcgacatctt ttcctgcgga gtcgtcctct acgagctgct
      181 caccggcgtg aagccgttcg aaggcccgac cgagacggtc gggtacaaga tatgccacga
      241 accccaccgc aatccttcgg agatcaatcc ccagggcgtt cccgaggtct tcgatgcaat
      301 cctcggtaag gcactcgcga agaagccgga agaccgctac gccaaggccc gcgaattcgc
      361 ggcggccctc gccaaggcct ttgagagccg cggcggtgcg gcggtcctga ccgaggcaac
      421 gatcctcccc acgatcattc accaggaccg gcgggacacg acttccccgc cgccgggatg
      481 ggagccgggc cagctgcatg ccctggagga gctgctcgcg ccctatgtcg gcccgatggc
      541 cagagtgctc gtcaagagat cggcgaagac caccaccgat ggccccaccc tcgtgcgcct
      601 tctcgccgcg agcgtgtcgg agcgggacgg aaaggcgttc gccgcggcgg cgctcgagaa
      661 ggttttcgcg atcgggcaac ccgaaggggc ggcggagcgg tcctctcctg gtatttggaa
      721 caaggcggtc gaggccgcag acgtcgacaa ggcggcaggg cagctcgcac cttacgtggg
      781 cccgatcgcc aaggtgatgg taaaaaaggc cgccgcgcag acgcgcgacc tgaaggcgtt
      841 ctatgagcgg ctcgcggaca acctgtccga cccggacgag cgggccgagt ttctcaaaaa
      901 ctcgggctac ggagaatagt ttgctctagg ccttcagcgt cggcaggagc ttcaaggcgt
      961 tttctcgctc gatcgcccgc aagtccccag gattgaagcg gaacgcggcg agcccttcga
     1021 tttcctcggc tccgtcgcgg aacggaaagt cggtgccgta aagcacctgc gacggcgtca
     1081 cgatcttcat cagcgccgcg agcgcgcccg cgtggttccc ttgagccgtg tcgtagtgga
     1141 acttcttcag ctcgtacatc acgccctttg gcacgcactc ctttgctttt tccttcatgt
     1201 ctaactcgtg gcgcgtgaag cggctgagga ggaagggcat cgtgccgccg ctgtgcgaga
     1261 agatccagcg catgtcgggg tagcgcgccg ccgtgccgct gaagacgagg ctcgcgatgg
     1321 tgcgcgtcgt gtccgtcgcg tattcgacga ccgctggcgg cacctcggtt ttgacgttgc
     1381 ggcagcagtc tggcgaaagc ggatgcgtgt agattaccgc cttcctccgg ttgagctcct
     1441 cccagatcgg cgcgaaggag gcatccccaa ggtatttgtc ctggtagctc gtcatcaggc
     1501 cgatgccctc cgccttgagc gtatccaggg cgtagccgat ttccttcagt gctccttcgg
     1561 tgtcgagaag cggcagggtc gcgaaactcg cgaagcggcc cttgtggtcg cgcgcgagct
     1621 gtgcgccgta ttcgttcgag aggcgcgcga tcttgcggcc tttctcgacc tcgttggtca
     1681 cggcgcccgg ctggatgagc gagagcaccg acacggcgat gccgctcttg tccatgtctt
     1741 cgatcgacat ggcgggcgac cacttaaagg tagagcctgc tcgccgcccg ccgacttcct
     1801 cgagataccc cgggtacatg aggtgatggt gaacgtcgat acggtgcgcc ttcgcgcccg
     1861 cctgcgccat cagctcgccc gggagcatcg cggaggcgcc gagcgcggcg agcttgccga
     1921 ggaaatcgcg ccgcggcgga cagcaggcgc aggacgggcc cggacggccg cctggagcgt
     1981 ttttagaagt ggacatcgta gacttcctcc tgtgagagct tcgcgaaaag tatcctatca
     2041 tggcaatcgg ggcgatttgg cggcggaggg acgcgatgaa ttcgttgcaa tacgcgtggc
     2101 tcgcaggttt gctccttgcc gcttccgcat gggcgcagga tccggcgccc ggatacccgg
     2161 gccgcccgat ccgggtcatc gttccattcc ccgcgggcgg ggcggccgac gcgctgccgc
     2221 gcatcgtcgg tgaaaagctc gcggcgcgct gggggcagcc cgttgtcgt
//
LOCUS       DASPZD010000346         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_689852, whole genome shotgun sequence.
ACCESSION   DASPZD010000346 DASPZD010000000
VERSION     DASPZD010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689852"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1381
                     /locus_tag="VEM33_06745"
     CDS             101..1381
                     /locus_tag="VEM33_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYS51559.1"
                     /translation="MFFSLFGRKKTESKKAPITRQPGKPSPEKSATPVASSQAGEDSL
                     DFSAYVPPPPLQVPPASAPAPQSAPQDAEAAPAEPPLDVSALVSSLAGQPDPVPSIVA
                     PIIEETATLFATGQAEQALARLSNAVREGGLGDSAQQGWLMLFDLYQHRGMKLEFEAL
                     ALEFVVKFERSPPAWIESDERRDPSLDTGGIAYFALGGKLTDASAPELEKLRIAASGH
                     QTLRVDCASLEGVDGPGCRLFRETLLALRGAGKEVLLTGEARLVQFLEEACQPGRKET
                     DSTVWALLLDVYRILDLKDPFEEAAVNYAVTFEVSPPSWERQPRAEATGSAVPRPVEA
                     ADQALVLSGELSGSGEALAKQLQDWAAANKMLVIDMSRATRVDLATAGVMLDVFSKLQ
                     GTGTTIQIRGANELIRTLFGVTGVSKVARIIARK"
     gene            1425..1961
                     /gene="hslV"
                     /locus_tag="VEM33_06750"
     CDS             1425..1961
                     /gene="hslV"
                     /locus_tag="VEM33_06750"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HYS51560.1"
                     /translation="MEQFRGTTIMSARRDGKVALGGDGQVTLGQVVVKASARKVRRLY
                     HDSILAGFAGGTADAFTLFERFEAKLEKHQGNLLRSAVELAKDWRTDRMLRRLEAMLA
                     VADRESSLIITGMGDVIEPELGLIAIGSGGPYAQAAARALLENTQLDAEAVVRKALSI
                     AADLCIYTNQQVVVETLD"
     gene            2023..3378
                     /gene="hslU"
                     /locus_tag="VEM33_06755"
     CDS             2023..3378
                     /gene="hslU"
                     /locus_tag="VEM33_06755"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HYS51561.1"
                     /translation="MSSMTPQDIVHELDKHIVGQTRAKRAVAIALRNRWRRQQVAEPL
                     RQEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGRDVDTIVRD
                     LVEISIKDTREAATKKVRRRAEDSAEERVLDVLLPSPRRTGAFAADGAELPASTGEEE
                     SATRQKLRKRLREGELDDKEVDIEVSAPQAHMEIFAPPGMEELTQQIQGMFQNLGGGR
                     RKLRRMKIREAMRMLTDEEAARLVNEEDLRAKALANVEENGIVFLDEIDKIASRGDTV
                     GVDVSRQGVQRDLLPIVEGTTVSTKYGMVKTDHMLFIASGAFHLSKPSDLIPELQGRF
                     PIRVELDSLSVEDFERILTATDACLTRQYQALLATEEVEVSFVADGIRRLAEIAWSVN
                     EKTENIGARRLYTVMEKLLEEISFEAGKRGKESFRIDAAFVDAKLKELAQSEDLARYV
                     L"
BASE COUNT          651 a         1084 c         1135 g          565 t
ORIGIN      
        1 catcgcaggg ttgccgcgtt gcacggtatc gagcaaagtg atacaacgaa accgtcattg
       61 gcatcgccag gcagattctc cgggaggcgg gtagttcctc gtgttctttt cactattcgg
      121 caggaagaag accgaaagta aaaaagcgcc gatcacccgg caaccgggca agccttcgcc
      181 ggagaaatcc gcgacacccg tcgcgagttc gcaagcgggc gaggactcgc tcgatttcag
      241 tgcctacgtt cctccgccgc cgctccaggt tccgccggct tcggcacccg caccgcaatc
      301 ggccccgcaa gatgcagagg ccgcgcctgc ggagccgcct ctggatgtgt ccgcgctggt
      361 ttcgtccctc gccggccagc cggatcctgt gcccagcatc gtcgcgccga ttatcgaaga
      421 gacggcgacc cttttcgcaa ccggacaggc cgagcaggcg ctggcaaggc tttcaaacgc
      481 ggtgcgtgaa ggcggactcg gggattcggc gcagcagggt tggctcatgc ttttcgacct
      541 gtaccagcat cggggcatga agctcgagtt cgaggcgctc gctctggaat tcgtcgtgaa
      601 attcgagcgc tcgccgcccg cgtggatcga gtcggacgag cgccgcgatc cctcgctcga
      661 cacgggaggc atcgcatact tcgcgctcgg cggcaagctg accgatgcga gcgcgcccga
      721 gctggaaaaa ctgcggatcg cagcctcggg ccaccaaacg ctgcgcgtgg attgcgccag
      781 cctggaaggc gtcgacggcc cgggctgccg gctgtttcgc gagacgctcc ttgccctgcg
      841 cggcgcgggc aaggaagtgc tgctcaccgg agaggcgcgg ctcgtccaat ttctggagga
      901 ggcctgccag ccggggagga aggaaaccga cagcaccgtc tgggcgttgc tgctggacgt
      961 ctaccggatc ctcgacctca aggacccgtt cgaagaggcc gcggtcaact atgccgtcac
     1021 cttcgaggtt tcgccgcctt cctgggaacg ccagcctcga gcggaggcaa cggggtccgc
     1081 ggtcccccgg ccggtcgagg ctgcggatca ggccttggtg ctctcggggg agctgagcgg
     1141 gtcgggcgaa gcactcgcca agcagttgca agactgggcc gcagcgaaca agatgctcgt
     1201 catcgacatg tcgcgagcga cgcgcgttga ccttgccacg gccggcgtta tgctggacgt
     1261 attctcgaag ctccagggga ccggcaccac gatccagatt cgcggtgcga acgagctcat
     1321 ccgtaccttg ttcggggtca ccggggtgag caaggtcgcc cgcattattg cgcgcaaata
     1381 gcttgatcga cggcgccctg gccccatctt ccctgaatcc gaacatggaa caattccgcg
     1441 gaaccaccat tatgtcggct cgccgcgatg ggaaagtcgc cttgggtggg gacggccagg
     1501 tcacgctcgg ccaagtcgtg gtgaaagcga gcgcgcgcaa ggtgaggcgc ctttatcacg
     1561 acagcatcct cgccggcttc gccggaggca cggccgacgc gtttacgctg ttcgagcgct
     1621 tcgaggcgaa gctcgaaaag caccagggaa atctgcttcg ctcggccgtg gagctcgcca
     1681 aggactggcg caccgaccgg atgctgcggc gcctggaggc gatgctcgcc gtggccgacc
     1741 gcgaatcctc gctgatcatc accgggatgg gcgacgtcat cgagcccgag ctgggattga
     1801 tcgcgatcgg atcgggaggc ccctacgcgc aggcggccgc gcgcgcgctc ctcgaaaaca
     1861 cgcagctcga cgcggaggct gtcgttcgca aggctctttc gatcgccgcg gacctgtgca
     1921 tctacaccaa tcagcaggtc gtcgtcgaaa cgctggacta gagcgccagc ggcccggggt
     1981 ctcgtagaat ccctggacct ccggctcccg gcttcccgcc ccatgtcttc catgactcca
     2041 caggatatcg tccacgagct ggacaagcac atcgtcggcc agactcgcgc gaaacgcgcc
     2101 gtggcgatcg ccctcagaaa ccgctggcgg cgccagcagg tggccgagcc actgcggcag
     2161 gagatcacgc ccaagaacat ccttatgatc ggaccgacgg gagtgggcaa gaccgagatc
     2221 gcgcgtcgcc tcgcgcggct cgccgacgca cctttcatca aggtcgaagc gacgaagttc
     2281 accgaagtcg gctacgtcgg ccgcgatgtc gatacgatcg tccgcgatct cgtcgaaatc
     2341 tcgatcaagg acactcgcga agcggcgacg aagaaggtgc gacgcagagc cgaggactcg
     2401 gccgaagagc gggtgctcga cgtcctgctg ccttcgcccc gccgcacggg cgctttcgcg
     2461 gcggacggcg ccgaacttcc ggcgagcacc ggcgaggaag agtccgcgac gcggcagaaa
     2521 ctccgcaaga gactgcgcga gggcgagctc gacgacaagg aagtcgatat cgaagtgtcc
     2581 gcgccgcagg cgcacatgga gatattcgcc ccgcccggga tggaggagct cacccagcag
     2641 atccagggca tgtttcagaa tctcggcggc ggcaggagaa aactgcgccg catgaagatt
     2701 cgcgaagcga tgaggatgct caccgacgag gaggcggctc gccttgtcaa cgaggaggac
     2761 ctgcgcgcca aggctctcgc caacgtcgag gagaacggca tcgtgtttct ggacgagatc
     2821 gacaagatcg cgagccgcgg ggacacggtc ggcgtcgacg tttcgcggca gggtgtgcag
     2881 cgcgacctgc ttcccatcgt ggagggcacc acggtctcca ccaagtacgg aatggtgaaa
     2941 accgaccaca tgctgttcat cgcgagcgga gcgtttcacc tttccaaacc ttcggacctg
     3001 attccggagc tgcaggggcg cttccccatt cgcgtcgagc tggattcgct ttcggtggag
     3061 gatttcgaga gaatcctcac ggcgaccgac gcctgcctga cccgccagta ccaggcgctg
     3121 ctcgccaccg aggaggtcga ggtctctttc gtagcggacg ggatccggcg tcttgccgag
     3181 atcgcctggt cggtgaacga aaagaccgag aacatcggcg cacggcgtct gtatacggta
     3241 atggagaagc tgctcgagga gatttcgttc gaggcgggca aacgcggcaa ggagagcttc
     3301 aggatcgacg ccgccttcgt cgatgcgaaa ctcaaggagc ttgcgcagag cgaggacctc
     3361 gcgcgctacg tgctgtgacg gttttgaagg tgcgtacgat tacgggagcg ctcgtgctgc
     3421 tgcttgccgc cgcag
//
LOCUS       DASPZD010000347         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_373855, whole genome shotgun sequence.
ACCESSION   DASPZD010000347 DASPZD010000000
VERSION     DASPZD010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373855"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3437)
                     /locus_tag="VEM33_06760"
     CDS             complement(<1..>3437)
                     /locus_tag="VEM33_06760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYS51562.1"
                     /translation="WAFVRGDSAEAVTSIIGKTRWELGEEPVNGTWEEHRKLLDARQP
                     FSDFQVRLVDENGAEHFFSSTGVPTFDSAGLFTGYRGLARRVTERKRAEQLLWLEHSV
                     ARSVTEADSVAAALKAVIRTVCEAEDWECGRFFRVDDEAGALRFAEAWSRPGPQFELY
                     IERSRRVAYIPGVGLAGKVWQSGEPLWVADVTRDARVWQQAIAVEAGLHGAFVFPVNS
                     GGKTIGILAFNSSEIREPDEHLMQAIHVIGAQIGQFVQRKQAEEVLRESEERFRNLTE
                     LSSDWYWEQDENFRFTMVSDNLHRRLGPLTQSVIGKTRWELPTLNMTEADWAAHRALL
                     QAHQPFRDLQWHRLDNAGKLQIASISGKPIFDSEGRFRGYRGIGRDITESKRSGEALL
                     RFRASMDMSDDMILLVDRKTMRFVDVNETVCRNLGYSREAFLAMTPEDILPVTRKELE
                     AAYDEMIATGSTQGMRSEYRCKDGSKLPFESRRRVMRSGDSWIIVVTARDIRERIAQE
                     EALRKSNERFDMAVRATNDVIWDWDLVTDEIWWNENFAKVFGYPREVADRSIKSWYEG
                     IHPDDQGRVIAGVHRVIGAGGEYWSDEYRFRRHDGSFVHVLDRGQVILDGAGRAVRMI
                     GAKADISSRKQAEERIHNQALRQRLIAEFGQQAFASSDVEDVLARAVELVTVALKADY
                     CSVLEYDESAKRLLIKAASGWPTETMERRTVAIRPGGRLEFVLSRREPLITEDLAKDE
                     RFSDSPLVRMGVRSGIQVPIFGTAGTFGILSAHARTALYFGVDDASFLQSVANILAVA
                     IERRSAEERLERLAQFDSLTGLPNRHLFYDRLLKTVAHARRSGQPMAVLFIDLDRFKL
                     VNDTQGHSAGDKLLKEAADRLAQCVRSGDTVGRFGGDEFGAIVSDLAKPGDAGVVAQK
                     VLDALTRPFKLDSHDTYVSASIGITLFPADGDNPEALVMNADTAMYRAKEQGRNTYQY
                     FTREMNERALARVQMEAALRQAIDQKEFLLHYQPKVALESRLICGFEALLRWRHPEKG
                     LVLPGEFVSVLEDTGLIVPVGERVLREVCAQIQAWQRSGLAVLPVTVNLSARQFQQKD
                     FESNVRHTLREAGIDPSLVQFELTESLLMSDPQGAARTLRGLKDSGVKISVDDFGTG"
BASE COUNT          545 a         1177 c         1085 g          630 t
ORIGIN      
        1 tagcccgtgc cgaaatcgtc caccgagatc ttcacgcccg agtccttcag accgcgcagc
       61 gtgcgcgcgg cgccttgagg atcgctcatc agcagagact cggtcagctc gaactgcacg
      121 agcgacggat cgatgcccgc ctcgcgcagg gtgtgccgca cgtttgactc gaaatccttc
      181 tgctggaact ggcgcgcgga gaggttcacc gttacgggca acaccgcgag cccgctccgc
      241 tgccatgcct ggatctgggc gcacacctcg cgcaacaccc tttcgcccac cggcacgatc
      301 aggcccgtgt cctcgagaac cgaaacgaat tcccccggca ggaccaggcc cttctcggga
      361 tgccgccagc gcagcagcgc ctcgaaaccg catatgaggc ggctctcgag ggccaccttg
      421 ggctggtagt ggaggaggaa ttccttttga tcgatcgcct ggcgcagcgc cgcctccatc
      481 tgcacgcgcg cgagcgcccg ctcgttcatc tcgcgggtga agtactggta ggtgttgcgg
      541 ccctgttcct tggcgcgata catggcggtg tcggcgttca tgaccagggc ttcggggttg
      601 tcgccgtcgg ccgggaacag ggtgatgccg atgctcgcgc tgacataggt gtcgtgcgaa
      661 tcgagcttga aaggccgggt gagggcgtcc aggacctttt gcgcgacgac gcccgcgtcc
      721 cccggtttcg ccaagtccga tacgatcgcg ccgaactcgt cgccgccgaa ccggcccacc
      781 gtgtcgccgc tgcgcacgca ctgcgcaaga cggtcggctg cttccttgag cagcttgtcc
      841 cctgcgctgt gcccctgggt gtcgttcacc agcttgaagc ggtccaggtc gatgaagagc
      901 accgccatgg gttgcccgct gcgtcgtgcg tgggccacgg ttttcagcag gcggtcgtaa
      961 aagaggtgcc ggttgggcag gccggtaaga gagtcgaact gcgcgaggcg ctcgaggcgc
     1021 tcctcagcgc tcctgcgctc gatcgcgacc gccagtatgt tcgccacgct ctgcaggaag
     1081 ctcgcgtcgt cgacgccgaa ataaagggcg gtgcgcgcgt gcgcgctgag tattccgaac
     1141 gttccggccg ttccgaatat ggggacctgg atgccgctgc gcacgcccat ccgcacgagg
     1201 ggtgaatccg aaaaccgctc gtccttcgcc aggtcttcgg tgatgagagg ctcgcggcgc
     1261 gacagcacga actcgaggcg gccccccggg cggatcgcca ccgtccggcg ctccatcgtc
     1321 tcggttggcc agcccgatgc cgccttgatc aggagtcgtt tggcgctttc atcgtactcg
     1381 agcacgctgc agtaatcggc cttgagcgcc accgtgacca gctccacggc acgggccagc
     1441 acgtcttcga cgtcggagct cgcgaacgcc tgctggccga actcggcgat gagccgctgc
     1501 cgcagcgcct ggttgtggat tctctcctcg gcctgcttgc gcgagctgat gtcggccttc
     1561 gcgccgatca tgcgcaccgc ccgtcctgcg ccgtccagaa tcacctggcc gcggtcgagc
     1621 acgtggacga aactgccgtc gtgccgccgg aaccggtact cgtcgctcca gtattccccg
     1681 cccgcgccga tcacgcgatg cactccggcg atgacgcgcc cctggtcgtc ggggtgaata
     1741 ccctcgtacc aggacttgat cgagcgatcc gccacttcgc gcggatagcc gaataccttg
     1801 gcgaagttct cgttccacca gatctcgtcg gtgaccaggt cccagtccca gatcacgtcg
     1861 ttcgtggccc gcaccgccat gtcgaagcgc tcgttgctct tgcgcagcgc ctcttcctgg
     1921 gcgatgcgct cgcggatgtc gcgggccgtg acgacgatga tccagctgtc cccggagcgc
     1981 atcacgcgcc gcctcgattc gaacggcagc ttcgagccgt ccttgcagcg atactcgctc
     2041 cgcatgccct gcgtggagcc ggtcgcgatc atctcgtcgt aggcggcctc cagctccttc
     2101 cgggtgacgg gcaggatgtc ttccggggtc atcgcgagaa acgcctcgcg cgagtacccg
     2161 agattccggc aaacggtttc gttcacatcg acgaagcgca tcgtcttgcg atcgacgagc
     2221 aggatcatgt cgtccgacat gtccatggag gcgcgaaagc gcagcaacgc ttccccgctt
     2281 cgcttgctct cggtgatgtc ccggcctatc ccgcggtagc cgcggaaacg cccctcggaa
     2341 tcgaagatcg gcttgccgct tatgctggcg atctggagct tgcccgcgtt gtcgaggcgg
     2401 tgccattgca ggtcgcggaa aggctggtgc gcttgcagca gcgcgcggtg cgccgcccag
     2461 tcggcctcgg tcatgttgag ggtgggcaat tcccatcggg tctttccgat gaccgattga
     2521 gtcaggggcc cgaggcgccg gtggagattg tcggaaacca tggtgaaacg gaagttctcg
     2581 tcctgctccc agtaccagtc cgatgagagc tcggtcaggt tgcggaagcg ctcttcgctt
     2641 tcgcgcagca cttcctccgc ctgcttgcgc tgcacgaact gcccgatctg cgcgccgatc
     2701 acgtggatcg cctgcatcag gtgctcgtcc ggttcgcgga tctcgctgct gttgaaagcg
     2761 agaatgccga tcgtctttcc tccggaattc accggaaaca cgaatgcgcc gtgcaatccc
     2821 gcttcgacgg cgatcgcctg ctgccacacg cgcgcgtccc gggtgacatc cgcgacccac
     2881 aagggctcgc ccgattgcca gacctttccg gccagcccga cgcccgggat gtacgccacc
     2941 ctgcgcgagc gctcgatata cagctcgaac tgcggcccgg gcctgctcca ggcttcggca
     3001 aagcgcagag cgccggcttc gtcgtcgacg cggaagaacc gcccgcactc ccagtcctcg
     3061 gcttcgcaaa ccgtgcggat cacggccttg agcgccgcgg cgacgctgtc ggcctcggta
     3121 accgagcgcg ccaccgagtg ctccagccaa agcagctgct ccgcgcgctt gcgctcggtg
     3181 acgcggcgag cgaggccgcg gtatccggtg aaaagtcccg cactgtcgaa cgtcggaaca
     3241 ccggtgctcg agaaaaagtg ctccgcgccg ttctcgtcga ccagccgcac ctggaaatcg
     3301 ctgaagggct ggcgcgcatc gagcagcttg cggtgttcct cccacgtgcc gttgacgggc
     3361 tcctcgccga gctcccagcg cgtcttcccg attatcgaag tgactgcttc ggcggaatca
     3421 ccgcgcacga aggccca
//
LOCUS       DASPZD010000348         4668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1002000, whole genome shotgun sequence.
ACCESSION   DASPZD010000348 DASPZD010000000
VERSION     DASPZD010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002000"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1442)
                     /locus_tag="VEM33_06765"
     CDS             complement(120..1442)
                     /locus_tag="VEM33_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYS51563.1"
                     /translation="MPQNQALAPSPTRFTTANIQPDLAFPFPNANTSGANDQTIRSVV
                     KPDLWGNTMRVRFSNVFGNQPLTLNSTTIALQEYAGNVVHGTVRPVTFGRSLSVTIPA
                     GQEMWSDAVHLSWVKHADDPLIQGRNLAISYSIQGDSGHMTYHSGANTTSYITGPGTG
                     DHTYDADGFAYEYTTASWFFLATLDVMASADTVVICAFGDSITDGTHTTFNGNDRWAN
                     VLSRRLHDAYGNKVSIVNEAIGGNRVINPVVANAASGPAAVDRLDRDVLGLSGLTHVI
                     WLEGINDLGAGYGAAANPVENPVIHTPQAIIAGYQDVVRRLHREGVKVYGATVVSALG
                     LNNPAQGWDLIHFPSFLATVDNGAAVEAGRLILNQFIRTPGNFDGVVDFDAATRDPDP
                     AALGNMKAKYLPNSQFTQLPWDYLHPNHAGYNAMGLAVDIAPFAPAHH"
     gene            complement(1879..2664)
                     /locus_tag="VEM33_06770"
     CDS             complement(1879..2664)
                     /locus_tag="VEM33_06770"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HYS51564.1"
                     /translation="MKSKRALATIALMLAFAARAAEPACSTHSGPHTTALVELYTSEG
                     CDTCPPADHWLSSLFGQGFRPDQVVPLALHVDYWDYIGWKDPFAKGEFSVRQRKLAQM
                     KRPVIVYTPQVLLQGQDFRRWSGGEFAEQVMRINSRPARARIALAIRAVAPGAIHADL
                     SAMLIDPAEKKNAAVYLAAYENKLASDVAAGENRGKRLEHDFVVREWIGPIGFGEGLK
                     IDERRALPLLPGTNAKNLGVAAFVQNRSTSEVLQALMLPVCES"
     gene            2745..3914
                     /locus_tag="VEM33_06775"
     CDS             2745..3914
                     /locus_tag="VEM33_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS51565.1"
                     /translation="MDSSVTRIFAPFYSSAFSWNFALGMTQLLIPLYARDLGYSGVAI
                     GSLIALPIIVQMIFNLIGGAWTDRLGGMNISLVAFAATAAAGVMFAASSSFAGLFAAQ
                     IMMIVARAVYWPASWTLVSQLPGERSGLMGTLNAITSSGQIAGTVGAGMIIAGWGFGA
                     GFWTVAAMGVLSFASGLVFRYAPPAREKAPLAMLATYRMLLGRRSIHYGIMCAYISAL
                     PFSLSVSFYPILLIEQGFGSEAAGWLVGMRALGAIAAGVVLARFVKHADESSVPLASA
                     LAIAASVGLVALFTNPAIIAFFLFGVGLGSGVMTIYFQILVSTLSSSETRGSAMALAG
                     MGWSISHISTPLVMGVLKDLYGIHAAFYALGAFAFLFSFALPPIHRWSLGGGKPR"
     gene            3980..4174
                     /locus_tag="VEM33_06780"
     CDS             3980..4174
                     /locus_tag="VEM33_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51566.1"
                     /translation="MNPIYKWMGIVLAVGLALMVVEYRFAKKKKEGFTPTDRQRILGI
                     FWIAIFLSLLVGGLMLISGD"
     gene            4179..>4668
                     /locus_tag="VEM33_06785"
     CDS             4179..>4668
                     /locus_tag="VEM33_06785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HYS51567.1"
                     /translation="MRVPLGVLDQSPVISGHSPAAAIAETVSLARLAEELGYHRYWLA
                     EHHAIAALADPSPEILVSRVACATSRIRVGTGGVLLPYYSPLKIAEQFRMLEALFPGR
                     IDLGIGRAPGGDPRTALALSDGEYPGAEHMPQQVQDLIGFLDRTLPAGHPYAKVKAQP
                     AGD"
BASE COUNT          749 a         1492 c         1525 g          902 t
ORIGIN      
        1 tcgagccggt tgcgccagta tccggggcgg cggtgcagat gcgcgaggtc gtccattggt
       61 tgctgtcgaa gcatggacga cctcgatccg ctctgcgctc gtcactcctc gcggtgcgat
      121 cagtggtggg cgggcgcgaa cggcgcaatg tcgaccgcca accccatcgc gttgtacccc
      181 gcatggttcg gatggagata gtcccacggc aactgcgtga actggctgtt gggcagatat
      241 ttcgccttca tgttacccag cgcggcggga tccggatcgc gtgtcgcggc gtcgaaatcc
      301 acgaccccgt cgaagttccc cggcgtgcga atgaattgat tgagaatcag gcgacctgcc
      361 tcgacggcgg cgccgttgtc caccgtggcc aggaagctcg ggaaatggat caaatcccaa
      421 ccctgcgccg gattgttcaa gcccagtgca gagaccacgg tggcgccata aaccttgacg
      481 ccctcgcgat gaagccgcct gacgacatcc tgatatccgg cgatgatcgc ctgcggcgtg
      541 tggatgacag ggttctcgac gggattcgcc gcagcgccgt acccagcgcc gaggtcgttg
      601 atgccctcga gccagatgac atgcgtcagc ccggacagcc cgagcacgtc tcggtcgagc
      661 cggtcgacgg cggcggggcc tgaggcggcg ttggcaacga ccggattgat gacccggttg
      721 ccgccgatcg cttcgttgac gatgctgacc ttgtttccat acgcgtcgtg cagccggcgc
      781 gacagcacgt tggcccagcg gtcgttgccg ttgaaggtcg tgtgcgtccc gtcggtgatc
      841 gagtcgccga aggcgcagat gacgaccgtg tcggccgagg ccatgacgtc gagcgtggcg
      901 aggaagaacc agctggcagt ggtgtactcg taggcgaacc cgtcagcatc gtaggtgtga
      961 tcgccggtgc ccggccccgt gatgtacgag gtcgtgttgg cgcccgagtg ataggtcatg
     1021 tggccgctgt cgccctggat ggagtagctg atcgccagat tgcgcccctg gatcagcggg
     1081 tcgtcggcgt gcttgaccca ggataggtga acggcgtcgc tccacatctc ctggccggcc
     1141 gggatcgtca ccgagagaga ccggccgaac gtcacgggcc tgacggtgcc atggacgaca
     1201 ttgcccgcgt actcctgcag tgcgatggtg gtcgagttga gggtcagcgg ttggttcccg
     1261 aagacattgg agaaccggac tcgcatcgtg ttaccccaca ggtcgggctt gacgacggag
     1321 cgaatggtct gatcgttcgc cccgcttgtg ttcgcgttgg gaaaggggaa cgcgaggtca
     1381 ggttggatgt tggcggtggt gaaccgcgtc ggagagggcg ccagcgcctg gttctgcggc
     1441 accggggcga cgtacacgaa gtatgccgcg ggagacgtgg cccagctggc cacccagtgc
     1501 tgggggcctc gatcatcatt cctgtcggcg agggccgccg gggcgatgcc cagactgagc
     1561 aggacgagca gtgccgccgc gacagaaaaa tgtcgacgcg cgcatctccc gagcgggaag
     1621 cgaactggac gactggtatt catcatgatg ccctcctttc aagaaacgga tagtagaatc
     1681 aattcgccgc tgaggtggag gtggtgaggg tcgaggatag tagacaattc gccgctgaag
     1741 ccatggcgcc tcgcatcgcc ccaccttgcg agtctagggg taaattgctg agcagtcaat
     1801 tacagccgca gcagcaaggc atggcctgtc gcacaccccg gtaggttcag gcgttgtcct
     1861 cgttcagcag cttagggctt agctctcgca aaccggcagc atcagcgcct ggagcacttc
     1921 gctcgtcgag cggttctgca cgaaagccgc aacgcccaag ttcttggcgt tcgtgcccgg
     1981 gagcagcggc agtgcgcgtc tctcatcgat cttcaggccc tcgccgaagc cgatcgggcc
     2041 gatccactcg cgcaccacga aatcgtgctc gagccgcttg ccccggttct cgccggcggc
     2101 cacgtcgctc gcgagcttgt tctcgtacgc ggccaggtac accgcggcgt tttttttctc
     2161 ggccggatcg atgagcatcg cggagaggtc ggcgtggatg gccccggggg ccaccgcgcg
     2221 gattgcgagc gcgatcctcg cgcgcgccgg gcgggaattg atgcgcatca cctgttctgc
     2281 gaattcgccg cccgaccagc ggcggaagtc ctgcccctgc agcagcacct gcggagtgta
     2341 gacgatcacc gggcgcttca tctgcgcgag cttgcgctgc cgcacggaaa attcgccctt
     2401 ggcgaacggg tccttccagc cgatgtagtc ccagtaatcg acgtgcagcg cgagcggcac
     2461 cacctggtcg ggtctgaatc cctgaccgaa gagcgaagag agccagtggt ccgctggagg
     2521 acaggtgtcg cagccttccg aagtatacag ctcgaccagc gcggtcgtgt gcggcccgct
     2581 gtgggtcgag catgccggtt cggcggcgcg cgcggcgaag gcgagcatga gcgcgatggt
     2641 tgcgagggca cgcttcgatt tcatggtgtc ttccgtcggc aggaaaggct catcccttac
     2701 agcggcgcat ggtacacgca actgcggact agtaccattt gcgtatggat tcttccgtaa
     2761 cccgcatctt cgcgcctttc tactcgagcg cgttcagctg gaactttgcg ctcgggatga
     2821 cgcaactgtt gatcccgctt tacgcgcggg acctgggtta ttccggtgtc gcgatcggca
     2881 gcctgatcgc gcttcccatc atcgtgcaga tgatcttcaa ccttatcggc ggagcgtgga
     2941 ccgaccgcct cggcggtatg aacatttccc tggtcgcgtt cgcggccacc gccgcggccg
     3001 gagtcatgtt cgccgcatcg tcgagcttcg cgggcctgtt cgccgcacag atcatgatga
     3061 tcgtggcacg cgccgtctat tggcccgcga gctggacgct cgtcagccag cttcccggcg
     3121 agcgcagcgg cttgatgggg acgctgaacg caatcacgag ctccggccag atcgcgggca
     3181 cggtcggcgc cgggatgatc atcgcaggct ggggcttcgg cgcgggtttt tggaccgtgg
     3241 cggcgatggg cgttctctct tttgcctcgg gcctcgtgtt ccgctacgcg cccccggccc
     3301 gggagaaggc cccgctcgcc atgcttgcga cctaccgaat gctgctcggc cggcgctcga
     3361 tccattacgg catcatgtgc gcctacatct cggctctgcc gttctccttg agcgtctcgt
     3421 tttatccgat cttgctgatc gagcaggggt tcgggtcgga agcggcaggc tggctcgtcg
     3481 gaatgcgtgc cctgggtgcg attgcggcgg gcgtcgtcct cgcgcgtttc gtgaagcacg
     3541 cggacgagtc ctccgtcccg ctcgcttcgg ccctggccat cgcggcctcg gtcggcctcg
     3601 tcgccctttt cacgaacccc gcgatcattg cgttcttcct cttcggcgtg ggactcggct
     3661 cgggcgtgat gacgatttat ttccagatcc tcgtgagcac cttgtcatcg agcgaaacgc
     3721 gcggatcggc gatggcgctc gccgggatgg ggtggagcat ctcgcacatc tcgactcctc
     3781 tcgtcatggg cgttctcaag gacctgtacg gcattcatgc ggccttctac gctctgggcg
     3841 cctttgcatt cctgttttcg ttcgcccttc ctccgatcca ccgctggtcg ctcggcggcg
     3901 gaaagccgcg ctgagcgcaa cgccttcccc gccctcgaag tccggtaaac tgagggcttc
     3961 gcctccgtgg agcccgaccg tgaacccgat ctacaagtgg atggggatcg tcctcgccgt
     4021 cggactggcc ctcatggtgg tcgagtatcg cttcgcgaaa aagaaaaagg aaggcttcac
     4081 cccgaccgac aggcagcgca tcctcggcat tttctggatc gcgatcttcc tcagcctcct
     4141 ggtaggcggc ctcatgttga tatcgggcga ctgaagggat gagggtgcct ctgggggtgc
     4201 tcgatcaatc ccccgtcatc agcggacaca gtcccgccgc agcgatcgcc gaaaccgttt
     4261 cgctcgcccg gctcgccgaa gagctgggct accaccgcta ctggctcgcc gagcaccacg
     4321 ccatcgcggc gctcgccgat cccagccccg agattctcgt ctcgcgcgtc gcctgcgcca
     4381 cctcgcgcat ccgcgtcggc acgggcggcg tgctcctgcc ctactacagc ccgctgaaga
     4441 tcgccgagca gttccgcatg ctcgaagccc tgtttccggg gcgcatcgat ctcggcatcg
     4501 gccgcgcgcc gggcggggac ccgcgcaccg cgctcgcctt gtccgacggc gaataccccg
     4561 gcgcggagca catgccgcag caagtccagg atctgatcgg ttttctcgac cgcacgctgc
     4621 ccgcgggtca tccatacgcc aaggtcaagg cacagccggc cggagata
//
LOCUS       DASPZD010000349         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1117136, whole genome shotgun sequence.
ACCESSION   DASPZD010000349 DASPZD010000000
VERSION     DASPZD010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117136"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VEM33_06790"
     CDS             complement(<1..855)
                     /locus_tag="VEM33_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51568.1"
                     /translation="MALTRRRFIQAAGLGASALGLPAIVRGQSGPIRVGLMTVKTGPL
                     ASGGIDMERALVQYLKERNTSMAGRKVDLFVGDSGGVPAQARTKIQELVERDRIQVMI
                     GPLDTASALAADDYIRQAQLLTLSVAAAEDMTQRKPNPWFTRGTSTSSQSAQPLADYC
                     AKTLKYKRMATIADDIAYGHEMCAGFQRVFEDAGGKIVQKLFSPLTVPDYGTYIAQLK
                     TNVDGIFLGFAGSNGFRFFRQFNEYGLRGKIAVVGGMTAFDEAVLRNMGDEALGIVTS
                     CWYSAEIEN"
     gene            complement(884..1546)
                     /gene="maiA"
                     /locus_tag="VEM33_06795"
     CDS             complement(884..1546)
                     /gene="maiA"
                     /locus_tag="VEM33_06795"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HYS51569.1"
                     /translation="MPMKLYGFWRSLATYRVRAALALKGIEVEQISINLLQGKQHAED
                     YKAINPQSVVPALVIDGGPPLFQSMAILEYLEETHPKPALLPKDPRGRARVRGLAQIA
                     VSDGHPLVVPRIRGYLEREMHQDEPARNKWLAHWTLEALKALEGHLGKESETARFCHG
                     DAPTIADICLVSQVFGAKFFNVETAGLPTAMRIFGECMKIDAFDRSQPAKQPDAPKNP
                     AH"
     gene            complement(1560..3272)
                     /locus_tag="VEM33_06800"
     CDS             complement(1560..3272)
                     /locus_tag="VEM33_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HYS51570.1"
                     /translation="MRIDWDVLIPMDDGLVLRADVYRPEGEGRFPVILSYGPYAKGLA
                     FQDGYPSAWQRMVAEHPDVACGSTNKYQNWEVVDPEKWVPEGYACVRVDSRGAGRSPG
                     YIDHFSPRETKDFYLCIEWAGVQPWASGKVGLNGISYYGINQWHVASLRPPHLAAMCI
                     WEGAADWYRDMTHHGGILSTFWANWYDMQVKTVQYGLGERGPRSRVTGELVCGDETLT
                     DRELQRNRCDFGDDILAHPLDDDYHKGRSPDWDKISVPFLSAANWGGQGLHPRGNFEG
                     FVRAASKEKWLEVHGIEHWTHFYTDYGVGLQKRFFAHHLKGEKNGWDRQARVQLLVRH
                     IDRFVERHENEWPIARTRWTKFHLDPAAHALVSESPGSAASVSYDASGDGMTFISAPL
                     GHETEITGPLAARLFVSSSTTDADLFLVFRVFSPDMREVVFMGAIDPHTPVAQGWLRA
                     SHRKLDPKLSTEYRPYHTHDELQPLKPGEVVRLDVEIWPTSIVVPAGHRLALTARGKD
                     YEWQKSTGARLSNFKNELRGCGPFLHDDPRDRPAAVFGGKVTIHAGPGRESYVVVPVV
                     PPKR"
BASE COUNT          562 a         1104 c         1089 g          614 t
ORIGIN      
        1 gttctcgatc tccgccgagt accagcagga cgtgacgatg cccaaagcct cgtcgcccat
       61 gttgcgcagc accgcttcgt cgaaggcggt catgccgccg acgacggcga tcttcccgcg
      121 gaggccgtac tcgttgaact gcctgaagaa gcggaagccg ttcgagcccg cgaagccgag
      181 gaagatgccg tccacgttgg tcttgagctg ggcgatgtag gttccgtagt cgggaacggt
      241 gagcggcgag aacagcttct gcacgatctt gccgcccgcg tcctcgaaca cgcgctggaa
      301 gccggcgcac atctcgtgtc cataggcgat gtcgtcggcg atcgtcgcca tgcgcttgta
      361 cttgagcgtc ttggcgcaat agtcggcgag cggttgggcg ctctgcgacg aagtcgaggt
      421 gccgcgggtg aaccagggat tgggcttgcg ctgcgtcatg tcctcggccg cggcgaccga
      481 cagcgtcaag agctgcgcct gccggatgta gtcgtcggcg gcgagagcgg aggcggtgtc
      541 gagcgggccg atcatgacct ggatgcggtc gcgctcgacg agctcctgga tcttggtgcg
      601 cgcctgcgcc ggcacccctc ccgagtcgcc gacgaagagg tccactttgc ggccggccat
      661 gctggtgttg cgctccttga ggtactggac gagcgcgcgc tccatgtcga tgccgcccga
      721 ggcaagcggc ccggtcttca ccgtcatgag gccgacgcgg atcggcccgc tttgccctcg
      781 cacgatcgcc ggcaagccga gcgcggaggc gccgagcccg gccgcctgga tgaaacgcct
      841 gcgggtgagt gccatgggga tccctccttt ggatcgtcgc gattcagtga gccgggttct
      901 tgggggcgtc gggctgcttc gccggctggg aacggtcgaa agcgtcgatt ttcatgcatt
      961 cgccgaaaat gcgcatcgcc gtcggcaggc cggcagtctc gacattgaag aacttcgcac
     1021 cgaagacctg ggacaccagg cagatgtcgg cgatcgtcgg cgcatcgccg tggcagaatc
     1081 tggccgtttc gctttccttg cccaggtgcc cttcgagggc cttgagcgcc tcgagcgtcc
     1141 aatgcgcgag ccacttgttg cgcgccggct cgtcctggtg catttcgcgc tcgaggtagc
     1201 cgcgaatccg cggaacgacc agcggatgcc cgtcgctcac ggcgatctgc gcaagccccc
     1261 gcacgcgcgc ccggcccctg ggatccttag gcagcagcgc cggcttggga tgcgtctcct
     1321 ccagatactc gaggatcgcc atcgattgaa acagcggcgg cccgccgtcg atcacgagcg
     1381 cgggcaccac gctctgcggg ttgatcgcct tgtaatcctc ggcgtgctgc tttccctgca
     1441 gcagattgat cgagatctgc tcgacctcga ttcccttgag cgcgagagcg gcgcgcacgc
     1501 ggtaggtggc gagagagcgc cagaacccgt agagtttcat cggcatcgca tgccccctct
     1561 cagcgtttcg gcgggacgac cggaaccacg acataggatt cgcgtcccgg tccggcgtgg
     1621 atcgtgacct tgccgccgaa gaccgcggcc ggacggtcgc gcggatcatc atgcaggaac
     1681 ggcccgcagc cgcgcaactc gtttttgaag ttcgagagcc tggcacccgt ggatttctgc
     1741 cactcgtagt cttttccccg cgccgtcagc gccagccggt gcccagccgg gaccacgatc
     1801 gaggtcggcc agatctcgac atcgagccgc accacctcgc cgggtttcag aggctggagc
     1861 tcgtcgtgcg tatggtaagg ccggtactcg gtggagagtt tcggatcgag cttgcggtgc
     1921 gaggcgcgca gccatccctg tgcaacgggg gtatgaggat ctatggcgcc catgaacacc
     1981 acttcgcgca tgtccgggga gaacacgcgg aagaccagga acagatcggc atcggtcgtc
     2041 gacgaggaca cgaacagcct cgcggcaagc ggccccgtga tctcggtctc gtgtccgagc
     2101 ggcgctgaaa tgaaggtcat gccgtcgccg gacgcgtcat agctgacgct cgccgcgctc
     2161 ccgggcgatt ccgaaacgag cgcgtgggcg gccgggtcga ggtggaactt cgtccagcgc
     2221 gtgcgcgcga tcggccattc gttttcatgc cgctcgacga agcgatcgat gtgccgcacc
     2281 agaagctgta cccgggcttg cctgtcccag ccgttcttct cgcccttcag gtggtgcgcg
     2341 aaaaaacgct tctgcagccc cacgccgtag tcggtataga agtgggtcca gtgctcgatg
     2401 ccatgcacct ccagccattt ctccttcgaa gctgcgcgca caaagccttc gaaattgccg
     2461 cgcgggtgca ggccctgccc gccccagttc gcggcagaaa gaaagggcac ggagatcttg
     2521 tcccagtccg gagaacgccc cttgtggtag tcgtcgtcga gcggatgagc gaggatgtcg
     2581 tcgccgaagt cgcagcggtt gcgctgcaat tcgcggtcgg tcagcgtttc atctccgcac
     2641 acgagctcgc ccgtgacgcg gctgcgcggg ccgcgctcgc ccagcccgta ctgcaccgtc
     2701 ttcacctgca tgtcgtacca gttggcccag aacgtcgata gaatcccgcc gtgatgcgtc
     2761 atgtcgcgat accagtcggc cgcgccctcc cagatgcaca ttgccgcaag atggggcggc
     2821 cgcagcgagg cgacgtgcca ctggttgatg ccgtaatagg aaatcccatt caggccaacc
     2881 ttgccgctcg cccagggctg cacgcccgcc cactcgatgc aaaggtagaa atccttcgtt
     2941 tcgcgcggtg aaaaatggtc gatgtagccc ggcgagcggc ccgcgccgcg ggaatcgacg
     3001 cgcacgcagg catatccttc cgggacccac ttctcgggat cgacaacctc ccagttctgg
     3061 tatttgttgg ttgacccgca ggcgacgtcg gggtgctcgg ccaccatgcg ctgccacgcg
     3121 ctcggatagc cgtcctgaaa cgcgaggcct ttggcgtagg gaccgtagct gagaatgacc
     3181 ggaaaccgcc cttcaccctc cgggcggtac acatccgccc ggagcacgag cccgtcatcc
     3241 atcgggatca ggacgtccca atcgatacgc atcccgtcgc gagcctcgct ccttgtcgac
     3301 gccatgtctg atccgcgatt tcggcttcgc ccgagctgtt atagttatcg cgccaatttg
     3361 accccacgc
//
LOCUS       DASPZD010000350         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1654135, whole genome shotgun sequence.
ACCESSION   DASPZD010000350 DASPZD010000000
VERSION     DASPZD010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654135"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VEM33_06805"
     CDS             complement(<1..448)
                     /locus_tag="VEM33_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYS51571.1"
                     /translation="MNRPISLILAALALALAVPAAAQEGTLKKIKDSGSITIGHRDAS
                     IPFSYYDDKQQPVGYAMELCLRIADAVKAELKLPKLDVKFQLVTSANRIPLMANGTID
                     LECGSTTNNLARQEQVWFTITHFVTANRWVAKKSANIRSLADLKGKT"
     gene            617..2062
                     /gene="aspA"
                     /locus_tag="VEM33_06810"
     CDS             617..2062
                     /gene="aspA"
                     /locus_tag="VEM33_06810"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HYS51572.1"
                     /translation="MSQAATRIEHDLLGDRAVPAEAYYGIHTLRALENFPITGTAISI
                     YPDLVRALACVKQAAALANNALGLLADDKTGVIVRACEEIREGELLDQYLVDVIQGGA
                     GTSTNMNANEVIANRALELLGRKRGEYQHLHPVEDVNMSQSTNDVYPTAVKVALHFAI
                     DRLGAAMQELRRAFDAKAREFADVLKMGRTQLQDAVPMTLGQELSTYAVMLQEDEERL
                     REAALLITEINLGATAIGTGINAHPAYAALVCQRLADITGIRLVTAPNLVEATQDAGA
                     YVQLSGVLKRIAVKLSKVCNDLRLLSSGPRAGLGEINLPPVQAGSSIMPGKVNPVIPE
                     VVNQIAFEVIGNDVTVSFAAEAGQLQLNAFEPVIAHSLFKSLMHLRNGCDTLRERCVT
                     GITANRERMAQTVRESIGLVTALNPYIGYSAASEVAREALATGKTVYDLVLEKKLLSK
                     EQLDEILRPEILTRPGRPVLPEPSTGKKAKL"
     gene            complement(2064..4354)
                     /gene="uvrA"
                     /locus_tag="VEM33_06815"
                     /pseudo
     CDS             complement(2064..4354)
                     /gene="uvrA"
                     /locus_tag="VEM33_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657953.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
BASE COUNT          731 a         1384 c         1484 g          755 t
ORIGIN      
        1 tggtcttgcc cttcaggtcg gcgagcgagc ggatgttcgc cgatttcttc gccacccagc
       61 ggttggcggt gacgaagtgc gtgatcgtga accagacctg ttcctggcgc gcgagattgt
      121 tggtggtgga gccgcactcc agatcgatcg tgccgttggc catcagcggg atgcgattcg
      181 ccgaagtcac gagttggaac ttgacgtcga gcttgggcag cttgagctcg gccttcactg
      241 cgtcggcgat gcgcaggcag agttccatgg cataacccac gggctgctgc ttgtcgtcgt
      301 agtaggagaa cgggatcgag gcgtcgcggt gtccgatggt gatcgagccg ctgtccttga
      361 tttttttcag agtgccttcc tgcgccgcgg cggggactgc gagggccaga gccagggcgg
      421 caagaatcag cgagatcggt cggttcatgt gggtctcctt gtgggccgtt cgagtcggcg
      481 cgtgtatggg tgggggaggc tgcacgattc tacgggagtc cccggttcct tgtccaaacg
      541 gcagggccga cggcgcaacg gcaaaacgcc tccggcggac tagaattcgg tctctgcctc
      601 cagaaggagt tgggacatga gccaggcggc gacgcgcatc gagcacgatc tcctcggcga
      661 ccgggcggtc cccgccgaag cctattacgg gatccacacg cttcgcgcac tggagaattt
      721 cccgatcacc gggactgcca tctccattta tccggacctc gtgcgcgcgc tcgcctgcgt
      781 caagcaggcg gcggcgctcg cgaacaatgc cctcggactg cttgcggacg acaagaccgg
      841 cgtgatcgtg cgggcctgcg aggagatccg cgaaggcgag ctcctcgatc aatacctcgt
      901 ggacgtcatc cagggcgggg cggggacttc gaccaacatg aacgcgaacg aggtcatcgc
      961 caaccgcgcg ctcgagctgc tcggccgcaa gcgcggcgag taccagcatc tgcacccggt
     1021 cgaagacgtc aacatgagcc agagcaccaa cgacgtctac ccgacggcgg tcaaggtggc
     1081 gctgcatttc gcgatcgacc gcctcggcgc ggccatgcag gagctgcgcc gcgccttcga
     1141 cgcgaaggcg cgggagttcg ccgatgtcct caaaatgggg cgaacgcagc tccaggacgc
     1201 ggtgccgatg acgctcggcc aggaattgtc cacgtatgcc gtaatgctgc aggaggacga
     1261 ggagcgcctg agggaggcgg cgctgctgat caccgagatc aatctggggg cgaccgccat
     1321 cggcaccgga atcaacgcgc accccgccta cgccgcgctg gtgtgccagc ggcttgccga
     1381 catcacgggc atccggctcg tgacggctcc gaatctcgtc gaagcgacgc aggacgcggg
     1441 cgcctacgtg cagctctcgg gcgtgctgaa acggatcgcg gtgaagctct ccaaggtctg
     1501 caacgacctg cggctgctgt cctcgggtcc ccgcgccggc ttgggcgaga tcaacctgcc
     1561 gccggtgcag gcgggatcgt cgatcatgcc cggcaaagtc aatccggtca ttccggaggt
     1621 cgtgaaccag atcgcgttcg aggtcatagg caacgacgtg accgtgagct tcgccgcgga
     1681 agccgggcag ctgcagctga acgccttcga gcccgtcatc gcccacagcc tgttcaagag
     1741 cctgatgcac ctgcgcaacg ggtgcgacac gctgcgcgag cgctgcgtga ccggaatcac
     1801 cgcgaaccgg gagcgcatgg cgcaaacggt ccgggagtcg atcggtctgg tcaccgcgct
     1861 caatccctat atcggctatt ccgccgcgag cgaggtggcg cgcgaggccc ttgccaccgg
     1921 caagacggta tacgacctgg ttctggagaa gaagctgctc tcgaaggaac agctcgacga
     1981 gatcctgcgg cccgagatcc tgacgcggcc ggggcgcccg gtgctccccg agccgtcaac
     2041 agggaagaag gcaaagctct agcctacgcc gcgcgcctct cccgtttttc cagcacgttg
     2101 cgcaggtagc ggccggtgaa gctcgcgtcc agggcggcca cgtcctcagg cgtcccctgg
     2161 gcgaggatgc gcccgccgcc ttcgccgcct tcgggcccga ggtcgatgat ccagtcggcg
     2221 gtcttgatca cgtccagatt gtgctcgatg acgaccacgg tgttgcccgc gtcgcgcagg
     2281 cggtgcagca ccgagagcag cagatcgata tccttgaagt gcaggcccgt ggtcggctcg
     2341 tccaggatgt agagcgtgcg tccggtgtcg cgcttggaga gctcgagcga gagcttcacc
     2401 cgctgcgcct cgccaccgga aagggtggtc gccggctgtc cgagctggat atagccgagg
     2461 cccacttcga gcagggtctg aagcttgcgc tccactgcgg ggaccgcgtt gaagaactcg
     2521 tacgcggatt ccaccgtcat ctgcaggacg tcgtggacgt tcttcccctt gaactgcact
     2581 tcgagggtct cgcggttgta gcgcttgccg tggcaggtgt cgcacggcac gtagatgtcc
     2641 gggaggaaat gcatttcgac cttgatgacg ccgtcgccct ggcaggcctc gcagcgcccg
     2701 cctttcacgt tgaaggagaa gcgcccggca ccgtagccgc gctcgcgcgc catagggacg
     2761 cccgcgaaaa gctcgcggat cggcgtgaag agcgcggtgt aggtcgccgg gttggagcgc
     2821 ggggtgcggc cgatcggacc ctggtcgacg ctcaccgcct tgtcgaagaa ttccagcccc
     2881 ttgatcgaat cgtgcgcggc gggctcgtgc accgagccgt agagctcgcg cgccaccgcg
     2941 tgatagagcg tgtcgttgat gagcgtcgat ttgcccgaac ccgagacgcc cgtcacgcac
     3001 acgaacaggc ccaccggcag ggcgagatcg atattcttga gattgttccc gcgcgccccg
     3061 cgcagccgaa acagccgcgt cttgtcgaaa cggcggcggg tctggggcag cgcgatctcg
     3121 agcgcgccgg agagatactg gccggtgagg gaggcggggt tcctgcggat ctgctcgggc
     3181 gtgccctccg cgaccaccag cccgccgtgc tctcccgctc ccggtcccat gtcgagcaca
     3241 tgatcggccg agttgatcgc ctcttcgtcg tgctcgacca cgatgacggt gttgccgagg
     3301 tcgcgcaggt gcttgagcgt ggcaaggagg cgcgcgttgt cgcgctggtg cagtccgatc
     3361 gagggctcat cgagcacgta catcaccccg gtgagtcccg agccgatctg gctggcaagc
     3421 cggatcctct gcgcctcgcc gcccgagagc gtgtccgcgg cgcggtcgag cgacagatag
     3481 tcgaggccga cgttgttgag gaagttgagc cgcgcaacga tctcgttgac gattttctcg
     3541 gcgatggcct gctttgcgcc cgagagcttg agccggcgga aaaattcgag cgcctgcttc
     3601 agggggaatc cgctcacctc gtaaatggcg cgctcgccga tcctgacgtg gcgcgcctcg
     3661 aggcgaagcc gcgttccctc gcacgagggg caggccttgg tgttccggta tttcgcgagc
     3721 tcctcgcgca ccacgagcga gtccgtttcc ttgtagcgac gctcgaggct gggcaggatg
     3781 ccctcgaagg cgtgctcctt cacgacggtg cggccgcgct cgctcaggta ggagaacggg
     3841 attttctccc gcgtgccgtg gagcaccagc gcctggaccg gctcgggcag gttttcgaac
     3901 ggggcctcga cgtcgaaccc gaaatgcgcg gcgagactcg tgagcatctg gaatagaact
     3961 ggttgcgccg gtcccagccc ttgatcgcgc cgctcgccag gctcaagctc gcgtgcgcga
     4021 ccacgcgctt gggatcgaag aaagtgatcg tgcccaggcc gtcgcattcg gggcaggccc
     4081 ccatcggatt gttgaaggag aacaggcgcg gctcgagctc cgacagcgag tagtcgcaca
     4141 cggggcaggc gtagcgcgcc gagaacaggt gctcccggcc cgaatcgagc tcgagcacca
     4201 cggcgcggcc gtcggcgtgc ctgagcgccg tttcgaacga ttccgccagg cgctgtttcg
     4261 cgtcggggcg caccttgagg cggtcgacga ccacgtccac cgaatgcttg acgttcttcg
     4321 tgagcctggg caccgagtcg atttcgtgaa cctt
//
LOCUS       DASPZD010000351         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1377120, whole genome shotgun sequence.
ACCESSION   DASPZD010000351 DASPZD010000000
VERSION     DASPZD010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377120"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VEM33_06820"
     CDS             <1..906
                     /locus_tag="VEM33_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HYS51573.1"
                     /translation="LGGTRAAGRLDVEGTVSGFDQNKAEGGKPFAPRAFTIVGKLRHG
                     QPIEVENFSFVKANTERTAKQAIPSPTMLLRGGRAAVSYEAYPDLEELYADIAEVYRE
                     ELRQLGEAGCRYVQLDDTNFAFLCDPKLADTFRRDGYEPDEMPERFANLINSAIAERP
                     AGMVAAIHLCRGNSMGQWAARGGYEPVAEVLLNKVDVDAYFLEYDDERSGGFEPLRFF
                     PGHSRKRVVLGLLSTKVPDLESKDALKRRIEAAAKIVPLENLCLSPQCGFASTFRGNP
                     ISEEVQRRKLERVVEVAIEVWGTAR"
     gene            1114..2163
                     /locus_tag="VEM33_06825"
     CDS             1114..2163
                     /locus_tag="VEM33_06825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYS51574.1"
                     /translation="MAVEHTGVAAQPRRLTSGAEESARLSALRSYCVLETGREARFDD
                     LTSLASTICETPVSLVSLVDTNRLFFKSAHGVDLREVPYPDFFCGHAIRQRELFVVPD
                     ANEDARFAKHPLVVESPRVRFYAGAPLITPQDYRLGTLCVIDFVPRQLQPKQLEMLRV
                     LGRQVMCQLELNLQAMRDPLTGLYNRRQLEEGLHREVLRARRVGGTVGVMAIDVDHFK
                     RVNDTLGHEVGDRALRGIADELASCVREEDIACRAGGEEFVIILPGAGKTALRSRAEA
                     VRRTIEQAQILAGAGTLKLTVSIGLASFPAYGDTGPAVLRAADVALYKAKAAGRNRVT
                     MCTPGVARSSPAKVA"
     gene            2256..>2452
                     /locus_tag="VEM33_06830"
     CDS             2256..>2452
                     /locus_tag="VEM33_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51575.1"
                     /translation="MSAGFKGRREDLRLVTGQGKYTSDWNLPGQLHASFLRSDRAHAE
                     IVSVDTASVLASSGVVAVFTGA"
BASE COUNT          443 a          762 c          813 g          434 t
ORIGIN      
        1 ctcggcggga cgagggctgc aggccgcctc gacgtcgaag gcacggtcag cggtttcgac
       61 cagaacaagg ccgagggggg caagcccttt gcgccgcgcg ccttcacgat cgtgggcaag
      121 ctgcgccacg gacagccgat cgaggtcgaa aacttcagct tcgtgaaagc gaacaccgaa
      181 cgcaccgcca agcaggcgat tccgtcgccc accatgctgc tgcgcggcgg ccgggcggca
      241 gtgagctacg aggcctaccc ggacctcgag gagctctacg ccgatatcgc cgaggtctac
      301 cgggaagagc tcaggcagct cggcgaagcc gggtgccgct atgtgcagct cgacgacacc
      361 aacttcgcct ttctctgcga cccgaaactc gccgacactt tccgccggga cgggtacgag
      421 cctgatgaaa tgcccgagcg cttcgcgaat ctgatcaatt cggccatcgc ggagcggccc
      481 gccgggatgg tggccgccat ccatctttgc cgcggcaatt caatgggcca gtgggcggcc
      541 cgcggcggct acgaacccgt cgccgaggtc ctgctcaata aggtcgatgt ggatgcatac
      601 ttcctcgaat acgacgacga gcgctcgggc ggcttcgagc cgctgcgctt ttttccggga
      661 cactcgcgca agagagtcgt gctcgggctg ctgagcacga aagtgcccga tctcgaatcg
      721 aaggacgcac tgaaacggcg catcgaggct gcagcgaaga tcgtgccgct ggagaatctg
      781 tgcctcagcc cccagtgcgg cttcgcgagc acgttccgag ggaatccgat ttcggaagaa
      841 gtgcagcggc gcaaattgga gcgcgtagtg gaggtggcga tcgaggtgtg gggtaccgcg
      901 cgctgaggat tcgaaaggct tagggcgcaa tatgttaccg ggaggggctc aggaacaaca
      961 ttcacttgat gcgcaaaacg agcgtcttcg atcggaaaaa tttcacgcca cggctctctt
     1021 tttccaagat gcgctcgaac ctcgtcgcgc gccttgttcc ggcgcctcgc tcgtagtaac
     1081 tttacgttcc ggggaggtca cgagaaatct cagatggcgg tcgaacacac aggcgtcgcg
     1141 gcgcagccgc gacgtcttac ttccggcgcg gaggagagcg cgcgtctttc ggctctgcgc
     1201 agctattgcg tgctcgaaac cggccgcgag gcccggttcg acgatctgac cagcctcgcg
     1261 tcgaccatct gcgaaacgcc ggtatcgctg gtgagcctgg tggacacgaa ccgcctgttc
     1321 ttcaaatcgg cgcatggcgt cgaccttcgc gaggtacctt atccggactt tttctgcggc
     1381 catgccattc gccagcgcga actcttcgtc gtccccgacg caaacgagga tgcgcgcttc
     1441 gcaaagcatc cgctggtggt cgaatcaccg cgcgtgcgtt tctacgccgg ggcacctctc
     1501 atcacgccgc aggactacag gctggggacg ctgtgcgtga tcgatttcgt tccccggcag
     1561 ctgcagccga agcagctcga aatgctgcgc gtccttggac ggcaggtgat gtgccagctt
     1621 gagctgaact tgcaggcgat gcgcgacccg ctcaccggcc tgtacaaccg ccgccagctc
     1681 gaggaaggcc tgcaccgcga agtcctgcgc gcgcgccgcg tcgggggaac ggtcggcgtc
     1741 atggcgatcg acgtggacca tttcaagcgg gtcaacgaca ccttgggcca cgaagtcggc
     1801 gaccgcgcct tgagggggat cgcggacgaa ttggcctcct gcgtgcgcga ggaagacatc
     1861 gcctgccggg ccggcgggga ggaattcgtc atcatccttc ccggggcggg gaaaacggca
     1921 ttgcgctcgc gtgcggaagc cgtgcgccgg accatcgagc aggcgcagat cctcgcaggg
     1981 gcggggacgc tcaaactgac ggtctcgata gggcttgcga gctttcccgc ctacggcgat
     2041 acgggaccgg ctgtgttgcg agctgcggat gtggcgctct acaaggccaa agcggccggt
     2101 cgaaaccggg tcaccatgtg cacgccgggc gtcgcgcgta gttcgccggc caaagtcgcc
     2161 tgacaatcta gtttcgaatt ccgcagattg atgcgtcgcg ggtcactgcc ccgcggctgc
     2221 tgagtgcatt cttgaatcag tccggaggca gaccgatgag tgcgggtttc aagggccggc
     2281 gcgaggatct gaggctggtc acggggcagg ggaaatatac gtccgactgg aatcttcccg
     2341 gacagcttca cgcctcgttc ctgcgctccg accgggcgca cgctgaaatc gtttccgtcg
     2401 acacggcgtc cgtgctggcg tcctccgggg tggtcgcggt gttcacgggc gc
//
LOCUS       DASPZD010000352         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1715326, whole genome shotgun sequence.
ACCESSION   DASPZD010000352 DASPZD010000000
VERSION     DASPZD010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715326"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VEM33_06835"
     CDS             <1..1144
                     /locus_tag="VEM33_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYS51576.1"
                     /translation="WEIPGAIRFNAMIPEGERSDLRRVAVGHEDVAFLQYTGGTTGVS
                     KGAVLLHRNILANLEQAAAWLRPFVDEDEPQAIVTALPLYHILSLTANCLLMMKAGGC
                     NILITNPRDIPGFVKELRKHKFTMFTGVNTLFNALMNHPDFAKIDFSGLKVALGGGMP
                     VQKAVADRWKKMTGCTLIEAYGLSETSPAVTINPLDLPDYNGSIGLPISSTEVVLRGD
                     DGKDAPSGSPGEIWVRGPQVMKGYWKSPAETAKVLGPDGFLATGDIGEMDEKGYVRIV
                     DRKKDMLLVSGFNVYPNEIEAVVAMLPGVLECAVIGVPDEHSGEVPKLFVVKRDASLT
                     EADIRQHCEANLTGYKRPKYIEFRKELPKTNVGKILRRALREEAKA"
     gene            complement(1150..1539)
                     /locus_tag="VEM33_06840"
     CDS             complement(1150..1539)
                     /locus_tag="VEM33_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS51577.1"
                     /translation="MAKIVIIEDDLTFLELLRVHLAGAGHEVLTAEDAVLGLRAVIDN
                     APDLILLDLSVPYLDGFEMIEALHSDPATRNIPVIVLTGRGDDETYTQARKLGAAQLL
                     TKPVARDLLMKAIDGQLDSNKQRSGKT"
     gene            complement(1647..2330)
                     /locus_tag="VEM33_06845"
     CDS             complement(1647..2330)
                     /locus_tag="VEM33_06845"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HYS51578.1"
                     /translation="MALTRTDLENDLLRATFRKFNPEVRVLSDEEQEASLGEVLDRHE
                     PGTDVWLFGYGSLVWNPIVHYEERRVARLHGFHRSFCLWSHVNRGSRQKPGLVLGLDS
                     GGSCRGVAYRIAGRRAEEELRLLWRREMVLGAYAPRWAKIDSGGETLQAIAFFVNRSH
                     PNYAGRLPLETVIKALVSARGQLGTPAEYLLETVHGLIEHGVRDPYLIELRKRVLAMH
                     PELAASHAR"
     gene            complement(2332..>2899)
                     /locus_tag="VEM33_06850"
     CDS             complement(2332..>2899)
                     /locus_tag="VEM33_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HYS51579.1"
                     /translation="ERASAVESKLGVGTALTESVARYYFKLMACKDEYEVARLYTDPA
                     FARKIEGMFEGGYKLKFHLAPPIFNKPDPRTGEARKSEFGPWMMAAFRVLAKLKRLRG
                     TPFDVFGASAERKAERRLIGEYEATVAELLEHLDGENRKLAVEIASVPEHIRGFGHVK
                     RRHLDEAKRKEVALLTQFRAHWSTARAA"
BASE COUNT          512 a          939 c          947 g          501 t
ORIGIN      
        1 ctgggagatc ccgggcgcaa tccgcttcaa cgccatgatc ccagaagggg agcggtccga
       61 tctcaggcgg gtcgccgtcg gtcacgaaga cgtcgcgttc cttcagtaca cgggaggcac
      121 gaccggtgtg tccaagggcg ctgtgctcct gcaccgcaac atcctcgcca acctcgagca
      181 ggccgcggcg tggttgagac cgtttgtcga cgaagacgag ccgcaggcga tcgtcactgc
      241 cttgccgctc tatcacatcc tctcactcac cgccaactgc ctgctcatga tgaaggcggg
      301 cggatgcaac attctcatca ccaatccacg cgacattccc ggattcgtga aggaattgcg
      361 gaagcacaag ttcaccatgt tcaccggggt aaacacgctc ttcaacgcgc tcatgaacca
      421 tcccgacttc gcgaagatcg atttctccgg cctgaaggtc gccctcggcg gcggcatgcc
      481 ggtgcagaag gcggtggcgg accgctggaa gaaaatgacc ggctgcacgc tcatcgaggc
      541 ctacggcctg tcggagactt cccccgcggt gaccatcaat ccgctggacc tcccggacta
      601 caacgggtcg atcggactgc cgatctcatc gacggaggtg gtgctgcgcg gagacgacgg
      661 gaaggacgcc ccctccggct cgcccggcga gatctgggtg cgcggccccc aggtgatgaa
      721 aggctattgg aaaagcccgg ccgagaccgc aaaggtgctc ggccccgacg ggttcctcgc
      781 caccggagac atcggcgaga tggacgagaa aggctacgtg aggatcgtcg accgcaagaa
      841 ggacatgctc ctcgtgtccg ggttcaacgt gtatccgaac gagatcgagg cggtggtcgc
      901 gatgctgccg ggcgtgctcg aatgcgcggt catcggcgtg cccgacgagc actccgggga
      961 agttcccaag ctcttcgtcg tgaaaagaga cgcatcgctc acggaagccg acatccggca
     1021 gcactgcgag gccaacctca ccggctacaa gcgacccaag tacatcgaat tcaggaagga
     1081 attgcccaag acgaacgtgg ggaagatcct gcgccgcgcg ctgcgcgagg aggcgaaagc
     1141 gtagggctct tatgttttcc cggagcgctg tttgttggaa tcgagctgcc cgtcgatcgc
     1201 cttcatcagg aggtcgcgcg cgaccggttt ggtcagcagc tgcgcggcgc cgagcttgcg
     1261 cgcctgcgtg taggtctcgt cgtcgccgcg tccggtgaga acgatcaccg ggatgttccg
     1321 ggtcgccggg tcgctatgca gcgcctcgat catctcgaat ccgtccaaat agggcaccga
     1381 gaggtcgagg aggatcaggt cgggtgcgtt gtcgatgacc gcgcgcaaac cgagcactgc
     1441 gtcctcggcc gtcagcacct cgtgccccgc gcccgcaagg tgcacgcgca agagttccag
     1501 gaacgtgagg tcgtcttcga tgattacgat cttggccaac ttgctcccct cgcgggcggc
     1561 gagcctcagc gttccacgct agtctgggcg cggacgggta ttctggcaac tacgcccatc
     1621 ggcctacgct gccagcgcgc agtaggctaa cgcgcgtgcg aggcggcgag ctcggggtgc
     1681 atggcgagca cgcgcttgcg aagctcgatc aggtacggat cgcgcacgcc gtgttcgatc
     1741 aatccgtgca cggtctcgag caggtattca gccggagtgc ccagttggcc acgcgcgctc
     1801 accagggcct tgatgacggt ctcgagcggg agcctcccgg cgtagttggg gtgagagcgg
     1861 ttgacgaaaa aagctattgc ctgcagcgtt tccccgcccg agtcgatctt cgcccagcgc
     1921 ggcgcgtagg cgccgagcac catttcgcgc cgccacagga ggcgcagctc ctcctccgcg
     1981 cgccggccgg cgatgcggta cgcgacgccg cggcagctgc cgccggaatc caggccgagg
     2041 accagaccgg gtttctgccg cgagccgcgg ttgacgtgcg accacaggca gaagctgcgg
     2101 tgaaagccgt gcagccgcgc tacgcgccgc tcttcgtaat ggacgatcgg gttccagacg
     2161 agcgagccgt agccgaacag ccatacgtcc gtaccgggct cgtgcctgtc tagaacctcc
     2221 cccagggagg cttcctgctc ctcgtcggag agcacccgca cctccggatt gaacttgcga
     2281 aacgtcgccc gcagcaggtc gttctcgagg tcggtgcggg tgagggccac gctacgcggc
     2341 tctggcggtg gaccagtggg cgcggaattg agtgagcagc gcgacttcct tcctcttcgc
     2401 ctcgtccaga tggcgccgct tgacgtggcc gaagccgcgg atatgctcgg gtacgctcgc
     2461 gatctccacg gcgagcttcc ggttctcgcc gtccaggtgc tcgagcagct cggcgacggt
     2521 cgcctcgtac tcgccgatca ggcgccgctc ggccttgcgc tccgcgctcg ccccgaagac
     2581 atcgaacggc gtgccgcgca ggcgcttcaa tttcgcgaga acgcggaacg ccgccatcat
     2641 ccagggtccg aactccgact tcctcgcctc gccggtgcgc ggatcgggct tgttgaagat
     2701 cggcggagca aggtggaact tgagcttgta gccgccttcg aacatgcctt cgatctttct
     2761 cgcgaatgcc gggtcggtgt agaggcgcgc gacctcgtat tcatccttgc aggccatgag
     2821 cttgaagtag tagcgggcga ccgattcggt gagcgcggtg ccgacgccga gcttcgactc
     2881 cacggcgctc gcgcgctcg
//
LOCUS       DASPZD010000353         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_458535, whole genome shotgun sequence.
ACCESSION   DASPZD010000353 DASPZD010000000
VERSION     DASPZD010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458535"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..138
                     /locus_tag="VEM33_06855"
     CDS             7..138
                     /locus_tag="VEM33_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HYS51580.1"
                     /translation="MHGEPVPVDTILRDRDRVEIYRPLQVDPKDVRRVRAGKKRAKG"
     gene            complement(142..627)
                     /locus_tag="VEM33_06860"
     CDS             complement(142..627)
                     /locus_tag="VEM33_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYS51581.1"
                     /translation="MNRAALFAVAFLAVSSAQGQVHKCVDAAGKVIYTQDPCPANTKP
                     GSISRSVIQAPAAAPTDSAAKGEGAKSSAPKTAAEQEQAFRKRQQDQAKAAKESDQKS
                     TEVQRKEENCRNARERLAQYDIGGRMTRINPQGERYYLEDAQIEQDKARARADVAQSC
                     Q"
     gene            760..2220
                     /gene="guaB"
                     /locus_tag="VEM33_06865"
     CDS             760..2220
                     /gene="guaB"
                     /locus_tag="VEM33_06865"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HYS51582.1"
                     /translation="MRSVQKALTFDDVLLVPAHSSVLPRNVSLKTRLTRNIQLNIPLV
                     SAAMDTVTESPLAIAMAQEGGIGIIHKNLTAKKQAAEVAKVKRYESGVLRDPVTVTPD
                     ITVRELLALTQQHRVSGFPVVEKGKVVGMVTNRDYRFETNLDQPVRAIMTPRDRLVYV
                     KEGASPEEARELLHKHRLERVLVVRDGFELRGLITVKDILKSSEHPNAAKDQQGKLRV
                     GAAVGVGEGTEERLAALVEAGVDVIVVDTAHGHSQGVLERLRWIKRNAGSAEVIGGNI
                     ATADGAKALVDHGADAVKVGIGPGSICTTRIVAGVGIPQVTAIQNVALALEKAGVPVI
                     ADGGVRFSGDVAKAIAAGAHSVMLGSLFAGTEESPGEIELFQGRSYKSYRGMGSLSAM
                     QQGAADRYFQDSSVHADKLVPEGVEGRVPYKGSVMRVIEQLMGGLRASMGYCGCEGID
                     AMRERAQFVEITAAGMRESHVHDVQIVKEAPNYQVE"
     gene            2220..>2573
                     /locus_tag="VEM33_06870"
     CDS             2220..>2573
                     /locus_tag="VEM33_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYS51583.1"
                     /translation="MHDKILILDFGAQYSQLIARRVREAQVYCELHPFDVDEKFIRRL
                     SPRGIILSGGPASVYENATPKAPDAVFKLGVPVLGICYGMQAMAAQLGGQVENSRVRE
                     FGYAEVRARGHSRLLE"
BASE COUNT          448 a          829 c          861 g          435 t
ORIGIN      
        1 gtcggaatcc atggagaacc ggttcccgtc gacacgatct tgcgggaccg ggaccgcgtc
       61 gaaatctacc gtccgctgca ggtcgatccg aaggacgtca ggcgggtgcg cgccgggaag
      121 aagcgcgcga agggatgagg ctcactggca ggactgggcg acgtcggcgc gcgcgcgcgc
      181 cttgtcctgc tcgatctgcg cgtcctcgag atagtaacgt tcgccttgcg ggttgatgcg
      241 cgtcatacgc ccgccgatgt cgtattgggc gaggcgctcc ctggcgttgc ggcaattctc
      301 ttccttgcgc tgcacctcgg tggatttctg atcggattcc ttcgcggcct tcgcctgatc
      361 ctgctggcgc ttgcggaacg cctgctcctg ctcggcggca gtcttgggcg cggagctctt
      421 cgccccttcg cctttcgccg cgctgtcggt gggcgcagcg gcgggcgcct gaatgacgct
      481 gcgcgaaatc gaccccggct tcgtgttcgc cggacagggg tcctgcgtat agatgacttt
      541 gcctgcagcg tcgacacact tgtgcacttg cccctgcgcg ctcgatacgg cgaggaatgc
      601 gacggcaaac agcgcagctc gattcatgac ggtttccctt ccgcgggtga ggcgcaacat
      661 atccagtttt aacgcaccag tcaatcgaag ggtgcacgcg aagcccgaga atttacgggc
      721 agatacgagc agcgtataat ccgacttttg ggtgtggcaa tgcgttcagt ccagaaggct
      781 ctcacctttg acgacgtcct tctcgtaccg gcccactcca gcgtcctgcc ccgcaacgtc
      841 agcctcaaga cccgcctgac gcgcaacatt cagctcaaca tcccgctcgt ctcggcggcg
      901 atggacacgg tgaccgagtc gccgctcgcg atcgccatgg cgcaggaagg cggcatcggc
      961 atcatccaca agaatctcac cgccaagaaa caggccgcgg aggtcgccaa ggtcaagcgc
     1021 tacgaatcgg gcgtcctccg cgatccggtg acggtgactc ccgatatcac ggtgcgagag
     1081 ctcctcgcgc tcacgcagca gcatcgagtg tcgggatttc ccgtcgtaga gaagggcaag
     1141 gtggtcggca tggtcacgaa ccgcgactac cgcttcgaga ccaacctcga ccagccggtg
     1201 cgcgccatca tgaccccgcg cgatcggctg gtatacgtca aggaaggcgc aagccccgag
     1261 gaagcgcgcg agctcctgca caagcaccgg ctcgagcgcg tgctggtggt gcgggacggc
     1321 ttcgagctgc gcggcctgat caccgtgaag gacatcctca aatcgagcga gcacccgaat
     1381 gcggcgaagg accagcaggg caagctgcgg gtcggcgcgg cggtcggagt cggcgaaggg
     1441 acggaagagc gcctcgccgc gctggtcgag gccggggtgg acgtcatcgt cgtcgacacc
     1501 gcgcacgggc attcgcaggg cgtgctcgag cggctgcgct ggatcaagcg caatgccggc
     1561 tccgccgagg tgatcggcgg caacatcgcc accgccgacg gcgcgaaggc gctcgtcgat
     1621 cacggggccg acgccgtgaa ggtcggcatc gggccgggat cgatctgcac gacgcgcatc
     1681 gtcgccggcg tcggcatccc gcaggtcacc gccatacaga acgtcgctct agcgctcgag
     1741 aaggcgggcg tcccggtcat cgcagacggc ggggttcgct tctcgggcga tgtcgccaag
     1801 gcgatcgcgg cgggcgctca tagcgtgatg ctggggagcc tcttcgccgg cacggaagaa
     1861 tcgcccggag agatcgaatt gttccagggc cgctcctaca agtcctaccg cggcatgggt
     1921 tcgctctccg cgatgcaaca gggagcggcc gaccgctatt tccaggactc gtcggtgcac
     1981 gccgacaagc tcgttcccga gggcgtggaa ggccgggtgc cttacaaggg ctcggtgatg
     2041 cgggtgatcg agcagttgat ggggggcctg cgcgcgagca tgggctactg cggctgcgag
     2101 ggcatcgacg ccatgcgcga gcgcgcccag ttcgtcgaga tcaccgccgc gggaatgcgc
     2161 gagtcgcacg tgcacgacgt ccagatcgtc aaggaagcac cgaactacca ggtagagtag
     2221 tgcacgacaa gatcctcatc ctcgacttcg gcgcgcagta ctcgcagctg atcgcgcgcc
     2281 gcgtgcggga agcgcaggtc tactgcgagc tgcatccctt cgacgtcgac gagaaattta
     2341 tccgccgcct gtccccgcgg gggatcattc tctccggcgg ccccgcctcg gtatatgaaa
     2401 atgccacgcc caaggcgccc gacgcggtct tcaagctcgg tgtcccggtg ctggggatct
     2461 gctacggcat gcaggccatg gcggctcagc tcggcggcca agtcgagaat tcccgggtgc
     2521 gcgagttcgg atacgccgaa gtccgcgcgc gcggccattc cagactgctc gag
//
LOCUS       DASPZD010000354         3944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1356871, whole genome shotgun sequence.
ACCESSION   DASPZD010000354 DASPZD010000000
VERSION     DASPZD010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356871"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2638
                     /gene="aceE"
                     /locus_tag="VEM33_06875"
     CDS             <1..2638
                     /gene="aceE"
                     /locus_tag="VEM33_06875"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYS51584.1"
                     /translation="ETQEWLDALESVLEREGPERAHYLLEKLVDKARRSGAYIPFSLN
                     TAYLNTIPVHMEERSPGDHALEERIRSFCRWNAMVMVAKTNKSDDELGGHIASFASVG
                     TLFGIGFNHFWHAPHEGHGGDLVYFQGHSSPGIYARAFLEGRLTETQLLNFRREVDGQ
                     GISSYPHPWLMPDFWQFPTVSMGLGPIQGIYMARFLKYLQARGLANTENRKVWVFCGD
                     GEMDEPESLGAIGLAAREKLDNLVFIVNCNLQRLDGPVRGNGKIIQELEADFRGAGWN
                     AIKLIWGSYWDPLFARDAEGRLLRVMEETVDGEYQNYKANDGAFVRKHFFGKDPKLLE
                     MVSRMTDEDIWRLNRGGHDPHKIYAAYAAAVRHKDQPTVVLAKTIKGYGLGKQGQAKN
                     PTHQLKKMDLATIREMRDRFNVPIPDDKLEELPLYKPKDDDPIMVYLQERRKALGGYL
                     PQRRRKADDVPAVPALSAFDQQLKGSEGREISTTMAFVRMLTALVRDKSIGRHIVPIV
                     PDEARTFGMEGMFRQLAIYSSEGQKYEPVDREQVMYYREDKAGQILEEGINEPGAFSS
                     WIAAATSYSHSNLVMVPFYIFYSMFGFQRVGDLAWAAGDQRARGFLLGATAGRTTLNG
                     EGLQHEDGHSHVLSSVIPNCISYDPTFAYELAVIIQDGLHRMVTQQEDVFYYVTVMNE
                     NYEHPAMPAGAEQAILKGMYLFREGSKKKKTRVQLLGSGTILREVIAAGELLDADWGV
                     AADVWSTPSFTELRRDGLAVERRNMLHPEAKPEIPYVTQCLEKHEGPVIAATDYIKTF
                     ADQIRSFLPGERVYRALGTDGYGRSDSRAKLRHFFEVNRYFVTVAALKALADQGEVKP
                     KVVSDAIKKYGIDPEKPDPVTV"
     gene            2703..>3944
                     /gene="aceF"
                     /locus_tag="VEM33_06880"
     CDS             2703..>3944
                     /gene="aceF"
                     /locus_tag="VEM33_06880"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HYS51585.1"
                     /translation="MGTPREVFVPDIGDFKNVDVVEVLVKPGDTVKPEQSLISVESDK
                     ATMEIPSPAAGVVKEMRVKTGDKVSQGSPILVLEETETAPAAPARSAPKSQEAPTAKP
                     GPVLAAVPAPVPAAPAVGRTEPVSASAQLPGTIPHASPSVRKFARELGVDLVRVKGSG
                     PKGRITQADVQEFVKGVMRGAPAVAAVSARGGLVLDLPEWPQVDFAKFGPVEAKPLSR
                     IRKLSGGFLHRNWVSIPHVTQHDEADITELEAFRKENAQVAEKGGFKLTMLAFLVKAS
                     VNALKEFPDFNASLDKTGENLIIKKYYHIGVAVDTPDGLVVPVIRDADRKGVFDLAKE
                     LAEISKAAREKKLKPDSMRGGTFSISSLGGIGGTSFTPIINAPEVAILGVARSQMRPV
                     HKDGAFIPRLILPFSLAYDHRV"
BASE COUNT          781 a         1209 c         1261 g          693 t
ORIGIN      
        1 ggaaacgcag gagtggctcg atgcgctcga gtccgttctc gaacgcgagg gtcctgagcg
       61 cgcccactac ctgctggaga agctcgtcga caaggcacgc cgttcgggag cctacattcc
      121 cttctcgctc aatacggcct acctcaacac catcccggtg cacatggagg agcgctcgcc
      181 gggggaccat gccctggagg agcgcatccg ctcgttctgc cggtggaatg cgatggtgat
      241 ggtggcaaag accaacaagt cggacgacga gctgggcggt cacatcgcga gcttcgcctc
      301 ggtcggaact ctgttcggca tcggtttcaa tcacttctgg cacgcgccgc acgaagggca
      361 cgggggggat ctcgtctact tccagggaca ctcgtcgccc ggtatctacg cgcgcgcatt
      421 tttggagggc cggctcaccg aaacgcagct cctgaatttt cgtcgcgagg tggatggcca
      481 aggcatctct tcctatcccc acccgtggct catgcccgat ttctggcagt tcccgaccgt
      541 ctcgatgggg ctcgggccta ttcagggcat ctacatggcg aggttcctga aatatctgca
      601 ggcgcgcggg ctggcaaaca cggagaaccg gaaggtgtgg gtgttctgcg gcgacggcga
      661 gatggatgag ccggaatcgc tcggcgcgat cggcctggcg gctcgtgaaa agctcgacaa
      721 cttggttttc atcgtgaact gcaatctgca gcgcctcgac ggtccggtgc gtggcaacgg
      781 caagatcatc caggagctcg aagccgattt tcgcggggcg ggttggaatg ccatcaagct
      841 catctggggc tcgtattggg atcctctgtt cgctcgcgat gccgaaggcc gactgcttcg
      901 tgtcatggag gagacggtcg acggtgagta tcagaactac aaggccaacg acggtgcctt
      961 cgttcgcaag cacttcttcg gcaaggaccc gaagctgctc gagatggtgt cgcgcatgac
     1021 cgacgaggac atctggcgcc tgaaccgggg cgggcacgac cctcataaga tctacgcggc
     1081 ctatgcagcg gcggtgaggc acaaggacca gcccacggtg gtcctcgcca agaccatcaa
     1141 gggctacggc ctgggcaagc agggtcaggc gaagaatccg acccaccagc tgaagaagat
     1201 ggacctcgcg accatccgcg agatgcgcga ccgcttcaac gtgccgattc ccgacgacaa
     1261 actcgaggaa ttgccgctct acaaacccaa ggacgatgat ccgatcatgg tgtacctgca
     1321 ggagcgcaga aaggcgcttg gcggctacct cccgcagcgc cgccgcaagg cggacgacgt
     1381 tcccgcggtg ccggcccttt cggcgttcga tcaacagctg aaaggctcgg aagggcgcga
     1441 gatctcgacc accatggcgt tcgtgcgcat gctgaccgcg ctcgtgcgtg acaagagcat
     1501 cggcaggcac atcgtaccga tcgttccgga cgaagcgcgc acattcggca tggaagggat
     1561 gttccggcag ctcgcgatct actcctcgga aggacagaag tacgagccgg tcgatcgcga
     1621 gcaggtcatg tactaccgcg aagacaaggc ggggcagatt ctcgaagaag gcatcaacga
     1681 gcccggcgca ttctcatcct ggatcgcggc ggcgacttca tacagccatt cgaacctcgt
     1741 gatggtgccg ttctacattt tctattcgat gttcggcttc caacgcgtcg gcgatctcgc
     1801 gtgggcggcg ggcgaccagc gggcgcgggg ttttctcctg ggtgccacgg cgggccgcac
     1861 gactctgaac ggcgaggggc tgcagcacga ggacggacac agccatgtgc tctcttcggt
     1921 catccccaat tgcatctcgt atgatccgac cttcgcctat gagctcgcgg tcatcatcca
     1981 ggacggcctg catcggatgg tcacgcagca ggaggacgtg ttctattacg tcacggtgat
     2041 gaacgaaaac tacgagcatc ccgcgatgcc ggcgggcgcg gagcaagcga ttttgaaggg
     2101 catgtacctc tttcgggaag gctcgaagaa gaaaaaaacg cgcgtgcagc tgctgggctc
     2161 tggaacgatc ttgcgcgaag tcattgctgc gggcgagctg ctcgacgccg actggggcgt
     2221 ggctgcggat gtgtggagca ctcccagctt tacggagctg cgccgcgacg ggcttgcggt
     2281 cgagcgccgg aacatgctgc atccggaggc gaagcccgag attccgtacg tgacccagtg
     2341 cctcgagaag cacgagggtc ccgtgatcgc ggccaccgac tacatcaaga cttttgccga
     2401 ccagattcga tcgttcctcc cgggagaacg cgtctaccga gccctcggca ccgatggata
     2461 cggccgctcg gattcccgcg ccaagcttcg tcacttcttc gaggtgaatc gttacttcgt
     2521 gacggtcgcc gcgctcaagg cgctggccga tcagggcgaa gtcaaaccca aggtcgtctc
     2581 cgatgcaatc aagaaatacg ggatcgatcc ggaaaaaccc gatccagtga ccgtctgatc
     2641 aagcgtgggc tcgccccttc ggcggcggcc agccccggca tccaatcgcg aaccgagcaa
     2701 gcatgggaac tcctagggaa gtcttcgtac ccgacatcgg cgacttcaag aacgtcgacg
     2761 tcgtggaagt cttggtcaag ccgggcgata cggtaaagcc ggagcagtcg ctcatcagcg
     2821 tggaatcgga caaggcgacg atggaaattc cctcgccggc cgcgggcgtg gtaaaggaaa
     2881 tgcgggtcaa gaccggcgac aaggtctcac aaggcagtcc gatcctcgtc cttgaagaaa
     2941 cagaaaccgc ccctgcggcg cccgcaagat cggccccgaa gtcccaggag gcgcctacgg
     3001 caaagcctgg cccggtgcta gccgccgtcc cggcaccggt tcccgcagcg ccggcggtcg
     3061 gccggaccga accggtctcc gcatccgcgc aactccctgg aacgatcccg cacgcaagcc
     3121 cctcggtgcg caagttcgcg cgcgagctgg gcgtcgacct cgttcgagtc aagggctccg
     3181 gcccgaaagg acgcatcacg caggcggatg tccaggagtt cgtgaagggt gtcatgaggg
     3241 gcgcgcccgc ggtcgccgcg gtgagcgccc gcggcgggct cgttctggat ctgccggagt
     3301 ggccgcaagt ggatttcgcg aagttcggcc cggtcgaggc gaaaccgctg tcgcgcatca
     3361 ggaagctgtc gggcgggttc ctgcatcgca actgggtttc catcccgcac gtcacccagc
     3421 acgacgaagc cgacatcacc gagctcgagg cttttcgcaa ggagaacgcg caggtcgcgg
     3481 aaaaaggagg gttcaagctc accatgctcg cgtttctggt gaaggcttcg gtgaacgcgc
     3541 tgaaggagtt tccggacttc aacgcttcgc tcgacaagac cggtgagaac ctgattatca
     3601 agaagtacta tcacatcggc gtcgcggtcg acacgcccga tggcttggtg gtgcccgtga
     3661 tccgcgacgc ggaccgcaag ggcgtcttcg atctggctaa ggagctggcg gagatttcca
     3721 aagccgcgcg cgagaaaaaa ctcaagcccg acagcatgcg cggcggtacc ttcagcatct
     3781 ccagcctcgg cggtatcggg ggcacatcgt tcacacccat catcaacgcg ccggaggtgg
     3841 cgattctcgg cgtggcgagg tcgcagatgc ggccggtcca caaggacggc gccttcatcc
     3901 cgcggctcat tttgccgttt tcgctggcgt acgaccatcg cgtc
//
LOCUS       DASPZD010000355         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_675915, whole genome shotgun sequence.
ACCESSION   DASPZD010000355 DASPZD010000000
VERSION     DASPZD010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675915"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VEM33_06885"
     CDS             <1..372
                     /locus_tag="VEM33_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51586.1"
                     /translation="MRGKGNLGELMQDMMAGVEAPTVDEAGVLLAYLQKHGQRPLDPK
                     KYPAVNLPEGQSFKLACQQCHVLPDPQRHKASEWPAVVARMEKNMEWMNRVVANEPDP
                     REIQLKVEEINAFLVKYARKG"
     gene            complement(374..991)
                     /locus_tag="VEM33_06890"
     CDS             complement(374..991)
                     /locus_tag="VEM33_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HYS51587.1"
                     /translation="MIELYTWSTPNGRKVSVMLEECGLPYNLHKINIGAGDQFAPDYV
                     AINPNAKIPSIVDPDGPDGKPMRMMESGAILIYLSAKTGKFLPRSDRGKYLALQWLMF
                     QMGHIGPMFGQVHHFLRSAKEQVPYAIERYTKEKDRLYGVLDRRLGEAEYLAGEYSIA
                     DIATYPWVARYEWHKTDLNAFPNVKRWFDAIGTRPAVKRGMAVPS"
     gene            1052..1246
                     /locus_tag="VEM33_06895"
     CDS             1052..1246
                     /locus_tag="VEM33_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HYS51588.1"
                     /translation="MLKWVLAVAIALVVLTLLSPWLSKYGLGRLPGDLTVRYKGRLYY
                     LPFASTIVLSLALTLLARLL"
     gene            complement(1180..2631)
                     /locus_tag="VEM33_06900"
     CDS             complement(1180..2631)
                     /locus_tag="VEM33_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HYS51589.1"
                     /translation="MEPQAETDMLWGGLRQNILAGTRLALFLPVRALDFRISIGQYVA
                     LAFASFAFWLAGGMLRAGFPGTVDFAALTAGLAEIPLVLGTCLLAARVFREEQLAVAF
                     AVLLAATDPVFEVAGAAVQAAAHLVAVELDADAVYWAFVAWGFAVIPRTLYVLTGWRG
                     RSSVLAFGLLAGLLAILVAAFPRSELWIAARDDRAQAARPRLTQEEVFHRQGFILDEQ
                     LAALSPERPGVVDLYFVGVAGDSGQDTFYLELVSVKELLEERFDAAGRSIALINNTAT
                     LKNYPIATASNLRATLAHLGSVINTDEDIVLLHIATHGDNDYRLALDLPPLELAQLTP
                     SALARMLADSGIKWKVIVISACFSGGYIEPLKDDNTLIITAADAFHSSFGCDYESDYT
                     WFSQAFYDEALRETFSFADAFEAAKETVDDRERAEGYPPSNPQMFAGAAMRKKLAALE
                     KRLAARATTEPPQRRRASSVRASESTIVLAKGR"
BASE COUNT          511 a          871 c          913 g          482 t
ORIGIN      
        1 atgcggggca agggcaatct gggcgagctg atgcaggaca tgatggcggg cgtggaggcg
       61 ccgaccgtgg acgaggccgg agtcctgctc gcctatcttc agaagcacgg tcagcggccg
      121 ctcgacccga agaagtatcc cgcggtaaat ctcccagaag gccagtcgtt caagctcgcc
      181 tgccagcagt gccacgtgct tcccgacccg caacgccaca aggcgtccga atggccggcc
      241 gtcgtagctc gcatggagaa gaacatggag tggatgaacc gcgtggtcgc caatgagccc
      301 gatccgcgcg agattcagct caaggtcgag gaaatcaacg ccttcctcgt caagtacgcg
      361 agaaagggct gattcaggaa ggcacggcca ttccgcgttt caccgcgggc ctcgtaccga
      421 tcgcgtcgaa ccagcgtttt acgttgggaa aggcgttcaa gtccgtcttg tgccactcgt
      481 agcgcgcgac ccagggatag gtggcgatgt cggcgatcga gtactcgccg gcgaggtatt
      541 ccgcctcgcc caggcgccgg tcgagaactc cgtagagccg gtccttttcc ttggtgtaac
      601 gctcgattgc gtagggcacc tgctctttcg ccgagcgcag gaaatggtgc acctggccga
      661 acatcggccc gatgtggccc atctggaaca tcagccattg cagcgccagg tatttgccgc
      721 ggtcgctccg cggcaggaat tttccggttt tcgccgagag gtagatgagg atcgcgcccg
      781 attccatcat ccgcatgggc ttcccgtcgg gtccgtccgg atcgacgata gagggaatct
      841 tggcgttggg gttgatcgca acgtaatcgg gagcgaactg atcgcccgcc ccgatgttga
      901 tcttgtggag gttgtaggga aggccgcatt cttccagcat cacggagacc ttgcgcccgt
      961 tgggcgtgct ccaggtgtaa agctcgatca tcgtttcctc gacgagtccg gaagccgcgc
     1021 agtgtatcgt gaagcccggt aaagtaaagc catgctgaag tgggtcttgg cggtcgcaat
     1081 cgcgctcgtg gtgctcacgc tgctttcgcc gtggctgtcg aaatacgggc tcggccggct
     1141 gccgggcgac ctcaccgtgc gctacaaagg gcggctctac tacctgccct tcgccagcac
     1201 gatcgtgctt tcgctcgccc tgacgctgct ggcgcgcctc ctctgaggcg gctccgtcgt
     1261 cgccctcgcg gcgaggcgct tctcgagcgc agcgagcttc ttgcgcatcg cggcgcccgc
     1321 gaacatctgc gggttggagg gcggatagcc ctcggcccgt tcccggtcgt ccacggtttc
     1381 tttcgcggcc tcgaacgcat cggcaaacga gaaggtctcg cgcagcgcct cgtcgtagaa
     1441 cgcctggctg aaccaggtgt agtcgctctc gtaatcgcag ccgaaggagg agtgaaaggc
     1501 gtccgcggcg gtgatgatga gggtgttgtc gtccttgagc ggctcgatgt accccccgga
     1561 gaaacacgcc gagatcacga tcaccttcca cttgatgccg ctgtccgcga gcatgcgcgc
     1621 gagggcactc ggggtgagct gcgcgagctc gagcggcggg agatccaggg cgagcctgta
     1681 atcattgtct ccgtgagtgg cgatgtgcag aagcacgatg tcctcgtcgg tattgatcac
     1741 gctgccgaga tgcgcaagcg tggcgcgcag gttgctcgcg gtggcgatgg gataattctt
     1801 cagcgtcgcg gtattgttga tgagcgcgat cgacctgccc gcggcgtcga aacgctcctc
     1861 gagcagttcc ttgaccgaaa ccagctccaa gtagaaggtg tcctgcccgc tgtcgcctgc
     1921 gactccgacg aaatacagat ccacgacgcc aggccgctcg ggactgagcg cggcaagctg
     1981 ctcatcgagg atgaatccct gccggtggaa cacttcctcc tgagtcagcc gcggacgcgc
     2041 ggcttgggct cggtcgtccc gggccgcgat ccagagctcg ctgcgcggga aagcggcgac
     2101 gaggatcgcg aggagccccg caagaaggcc gaacgccagc accgaggaac ggccgcgcca
     2161 gccagtgagg acgtaaaggg tgcgcgggat cacggcgaag ccccaggcga cgaacgccca
     2221 gtacacggca tccgcgtcga gctcgaccgc aaccaggtgc gccgccgcct ggaccgcggc
     2281 tcccgcgact tcgaaaacgg ggtctgttgc ggcgagcaat accgcgaacg ctactgcgag
     2341 ctgttcttcc cgaaagactc tcgcggcaag aagacaggtg ccgagcacaa gcgggatctc
     2401 agcgagcccc gcggtgaggg cggcgaaatc gacggtgccc ggaaaacccg cgcgcagcat
     2461 accgcccgcg agccagaacg caaagctcgc gaacgccaga gcgacgtact gcccgatcga
     2521 gatgcggaag tcgagcgcgc gcaccggcag gaaaagcgca aggcgcgtgc ccgcgaggat
     2581 attctgcctg aggccgcccc acagcatatc ggtttcggcc tgaggttcca tctcagatta
     2641 gtccttcgat tgcggcaatc cgcgcgcggc gcagctttgc ggcgatatcg gatttcgcgc
     2701 tccgggcagc gattttcgcc gcatcgaccc ccgcagccgc agcgagcgcc cgccgcagga
     2761 ccacgcgcgg catgtag
//
LOCUS       DASPZD010000356         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1157527, whole genome shotgun sequence.
ACCESSION   DASPZD010000356 DASPZD010000000
VERSION     DASPZD010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157527"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VEM33_06905"
     CDS             <1..701
                     /locus_tag="VEM33_06905"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYS51590.1"
                     /translation="QAPLAALLAPAYLAARAKLIDPLHSMGRARAGNPAGVSANHGVS
                     EPLELPATSHVSIVDAEGNAVALTASVESAFGNRQMVRGFFLNNELTDFSWAPEQDGK
                     PVANRVEPKKRPRSSMAPTMVFDEKGRLYMVIGSPGGHSIINYVALTLVNVLDWNMDI
                     QKAIAAPRMGSRNGPTELERGTKLERLAPALERMGHSVRIRPETSGLHGIVRTATGWA
                     GGADPRREGEALGD"
     gene            complement(731..1738)
                     /locus_tag="VEM33_06910"
     CDS             complement(731..1738)
                     /locus_tag="VEM33_06910"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HYS51591.1"
                     /translation="MRHFISTQDWTVEELQTLLDEAAGLRSSPMQPLLQGRTLALLFL
                     NPSLRTRASFQIGAAQLGATAIVLEPGNGVWGIEFDSGKVMDGKAEEHIVEVAEVLSR
                     YCDLIAMRAFPGFKNWQYDREDPLIKALARHSRVPVINMETIIHPCQELALAMTLQQR
                     LGRLKGRKLCLTWTWNPRPLSTAVANSVTMIATKYGMNVSLLCPSPDYVLDERFMKVA
                     HENAASSGGRLAVTHEISEGYADADVVYANSWGSLPYYGDPEKEWELSRAYGRFIVDE
                     RKMALTNNAIFSHCLPVRRNVEASDGVLDSPGNVAIDEAENRLHVQKALMLKLLGVAG
                     R"
     gene            complement(1800..2306)
                     /locus_tag="VEM33_06915"
     CDS             complement(1800..2306)
                     /locus_tag="VEM33_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYS51592.1"
                     /translation="MTIKVGDKLPDGKLSESTEYDAAAGCPINPKDLSVAEAAKGKKI
                     AIFAVPGAYTPTCSAKHVPSYVKNYGQLKAKGVDEIWCVATNDAFVMAAWGRDQKAGG
                     KVRMLGDGSGEWTKKLGLSLDLTARNMGVRSQRYSMLVENGVVKKLNIEAPGKYEVSG
                     AETMLAQI"
BASE COUNT          410 a          795 c          745 g          470 t
ORIGIN      
        1 tgcaagcgcc gctcgcggcg ctgctggcac cggcttacct cgctgcgcgc gcgaagctca
       61 tcgacccgct gcattccatg gggcgggcgc gcgcgggcaa tcccgccggt gtttcggcga
      121 accatggagt ctcagagccg ctcgagctcc ccgcgacctc gcacgtctcc atcgtcgacg
      181 ccgagggcaa cgccgtggcg ctgaccgcga gcgtcgagtc cgcgttcggc aaccgccaga
      241 tggtgcgcgg gttcttcctc aacaacgagc tgaccgattt ctcctgggcg ccggagcaag
      301 acggtaagcc cgtcgccaac cgcgtcgagc cgaagaagcg cccgcgcagc tcgatggcgc
      361 cgaccatggt gttcgacgaa aaaggaagac tctacatggt gatcggctcg cccggcggcc
      421 actcgatcat caactacgtg gccctgacgc tcgtcaacgt gctcgactgg aacatggaca
      481 tccagaaggc catagccgcc ccgcgcatgg gcagccgcaa cggcccgacg gaactggagc
      541 gcggaacgaa gctcgagcgg ctcgcgcctg ccctcgagcg catgggccac agcgtgcgca
      601 tccggccgga aacgagcggc ctgcacggca tcgtgcgcac ggcgacgggc tgggcgggcg
      661 gcgccgatcc gaggagagag ggcgaggcgc tgggggattg aagtttttct gagagcgaag
      721 cgacgtcgtt tcagcgtcct gcaacgccca ggagcttcag catcagagcc ttctgtacgt
      781 gaaggcgatt ctcagcttca tcgatcgcca cgttacccgg actgtccagg acgccatcgc
      841 ttgcttcgac attgcggcgc acgggcaggc agtggctgaa tatcgcattg ttggtcaacg
      901 ccatcttgcg ctcgtcgacg ataaagcgtc cgtacgccct cgacagttcc cactcctttt
      961 cggggtcacc gtaatacggc agcgatcccc agctgttcgc atagaccacg tccgcatccg
     1021 catagccctc gctgatctcg tgggtaactg ccaagcgacc tccgctggac gccgcgtttt
     1081 catgcgccac cttcatgaag cgctcgtcga gcacgtagtc ggggcttgga caaagcaggc
     1141 tcacgttcat cccgtacttc gtggcgatca tcgttacgga gttggccacc gcggtgctca
     1201 agggccgcgg gttccacgtc catgtcaaac acaacttcct tcccttcagc cggcccagcc
     1261 tctgctggag cgtcatggcc agcgccaatt cctggcacgg gtggatgatc gtctccatgt
     1321 tgatgacggg aacccgcgaa tgccgggcga gcgccttgat gagcgggtct tcgcggtcat
     1381 actgccaatt cttgaaaccg gggaacgcgc gcatcgcgat gaggtcgcag tagcgtgaca
     1441 gcacctcggc gacttcgacg atgtgctctt cggccttgcc gtccatgacc tttcccgaat
     1501 cgaactcgat cccccacacg ccgttgccgg gttccaggac gatcgccgtg gcgcccagct
     1561 gagctgcgcc gatctgaaac gaggccctcg tgcgcagcga aggattcaga aacagcagag
     1621 cgagggtcct gccttgcaga aggggctgca tgggactgga tctgaggccg gccgcttcat
     1681 cgaggagcgt ctgcaattcc tcgactgtcc aatcctgagt actgatgaag tgtcgcattg
     1741 cggcctcatc cgaaaccggc tacgagatcc gcggcgcgag tgcacccgct gcggccgttc
     1801 tagatctgcg ccagcatcgt ctccgcgccg ctcacttcgt atttcccggg tgcttcgatg
     1861 ttcaacttct tcaccacgcc gttctcgacc agcatcgagt agcgctgcga gcgcacgccc
     1921 atgttgcgcg ctgtgaggtc gagcgagagg ccgagcttct tggtccattc gccgctgccg
     1981 tcgccgagca tgcgcacctt gccgcccgcc ttctggtcgc ggccccaggc ggccatcacg
     2041 aaggcgtcgt tggtcgccac gcaccagatc tcgtccacgc ctttcgcctt gagctggccg
     2101 tagttcttca cgtagctcgg gacgtgcttc gccgagcagg tcggcgtata cgcgcccggc
     2161 accgcgaaga tcgcgatctt cttgcctttg gccgcttcgg cgacgctcaa gtccttgggg
     2221 ttgatcgggc atcccgcggc ggcgtcgtat tcggtggatt cggacagctt gccgtcggga
     2281 agtttgtcgc ctactttgat ggtcatttcg gtctccgcag tttcgtgttt tttggagcgg
     2341 ctaggatcgc gcagttgagc ggcaaagtca aaatgcttgc ccgttccacg gcggctggct
     2401 atattcccgg ccatgcagac
//
LOCUS       DASPZD010000357         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_993977, whole genome shotgun sequence.
ACCESSION   DASPZD010000357 DASPZD010000000
VERSION     DASPZD010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993977"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VEM33_06920"
     CDS             <1..722
                     /locus_tag="VEM33_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS51593.1"
                     /translation="AYEEPFGKGGKFKGIQVVPMEDYVAVVRELNRLGWRVATHAVGD
                     AAIDQVLDAYAAADRDRSIRGRRWSVEHFFIGRPDHYPRVKALELVISAQDHLYRAGP
                     SLVKYWGRERAEQVTPVRSFLDHGILVAGGTDSPVIPYNPFWAMYHFISRETISDGVY
                     GASQRITREEALRVFTINNAKLTFEENLKGSIERGKLADLVVLPADILMIPEKQIETL
                     KPVATMVGGRFVYSDPAARLE"
     gene            810..1178
                     /locus_tag="VEM33_06925"
     CDS             810..1178
                     /locus_tag="VEM33_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51594.1"
                     /translation="MKNAVVWSEIAVSDLKRATAFYEKVLDGKLKAENFGPYEIALFP
                     HAADGIGGCLMKGEGYEPSAKGTVTYLAAEPGIDAALKRATAAGAKVLMPKMALPGNQ
                     GYIAHVSDCEGNRIGLHAMS"
     gene            1186..1866
                     /locus_tag="VEM33_06930"
     CDS             1186..1866
                     /locus_tag="VEM33_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HYS51595.1"
                     /translation="MRRADRLFQIVQFLRGRRLTTAAFLAGRLGVSLRTVYRDVRDLS
                     LSGVPVEGEAGVGYRLRAGFDVPPLMFTLNEIEALAVGARMVEAWGGPVLADSARTAI
                     EKIVAALPADRRIAVEKTRLYAPGFHVDGRAMRQLESVREAIGSRRVLRLDYRDSGGK
                     ASERDVWPLALYYWGRTWTIGAWCERRTDFRNFRVDRIAGLETLERRYPDEAGKRLAD
                     FIRAMEAR"
     gene            complement(1806..>2212)
                     /locus_tag="VEM33_06935"
     CDS             complement(1806..>2212)
                     /locus_tag="VEM33_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51596.1"
                     /translation="APRAGDRFPWLRLRFQANGPVEDMFQKLDDTRYNLIVFGQPAAP
                     GEAPGLGDLLSTHVIPDDAHNARELARVRIPGQAFYLLRPDGHVGLAGARLAPDAVTR
                     YLSDGGIRTAGSLHRASIARMKSASRLPASSG"
BASE COUNT          369 a          739 c          765 g          339 t
ORIGIN      
        1 ccgcctacga ggaacctttc ggcaagggtg gcaagttcaa ggggattcag gtcgtgccga
       61 tggaggacta cgtcgccgtc gtgcgcgagt tgaaccgcct cggctggcgg gtcgccacgc
      121 acgcggtggg cgacgccgcg atcgaccagg tgctggacgc ctacgccgct gcagaccgcg
      181 accgctcgat acgcggcagg cgctggtcgg tcgagcactt cttcatcggc cggcccgacc
      241 attatccgcg cgtaaaggcg ctcgagctcg tcatctcggc gcaggaccac ctgtaccgcg
      301 ccggcccgag cctggtcaag tactggggcc gcgagcgcgc cgagcaggtg acgccggtgc
      361 gcagcttcct cgatcacgga atcctcgtcg ccggcggcac cgattcgccg gtgattccct
      421 acaacccgtt ctgggcgatg taccacttca tctcgcgcga gacgatttcg gacggcgtgt
      481 acggcgccag ccagcgaatc accagggaag aggcgctgcg cgtcttcacg atcaacaatg
      541 ccaagctcac cttcgaagag aacctgaagg gctcgatcga gcgcggcaag ctcgccgacc
      601 tggtcgtcct tccggccgac atcctcatga ttcccgagaa gcagatcgag accctgaagc
      661 cggtcgcgac catggtgggc gggcgctttg tgtacagcga cccggcggcg cggctcgaat
      721 agccgacggc tgctgacaca acgctgtcag caggggggcc tcaagctttc aggcgtgcga
      781 atcccgcact tcactcgaag gagactgaaa tgaagaacgc agtggtgtgg agcgaaatcg
      841 cggtatccga cctcaagcgc gcgaccgctt tctacgagaa ggtgctcgac ggcaagctga
      901 aggccgaaaa tttcggcccc tacgaaatcg ctctgttccc gcacgccgcg gacggaatcg
      961 gcggctgcct gatgaagggc gaaggttacg aaccctctgc gaagggcacg gtgacctatc
     1021 tcgcggccga gccggggatc gacgcggcgc tcaagcgcgc taccgccgcc ggcgcgaagg
     1081 tgctcatgcc gaagatggcg ctgcccggca accaaggcta catcgcccac gtgtcggact
     1141 gcgagggcaa ccgcatcggg ctgcacgcga tgagctagcg aggccatgcg gcgcgccgat
     1201 cgcctcttcc agatcgtcca gttcctgcgc ggccggcgtc tcacgacggc ggcgtttctg
     1261 gcgggccggc tcggcgtgtc gctgcgcacg gtctatcgcg atgtgcgcga cctgtcgctc
     1321 tccggcgtcc cggtcgaggg cgaggcgggc gtcggctacc ggctgcgcgc gggcttcgac
     1381 gttccgcccc tcatgttcac tctgaacgag atcgaggcgc tcgcggtggg cgcgcgcatg
     1441 gtcgaggcct ggggcgggcc ggtgctcgcc gactcggcgc gcaccgcgat cgagaagatc
     1501 gtcgccgcgc tgcccgcgga ccgccgcatc gcggtggaga agacgcgcct ctacgcgccg
     1561 gggttccacg tcgacggccg ggcaatgcgg cagctggaat cggtgcgcga ggcgatcggc
     1621 tcgcgccgcg tgctgcgcct cgattaccgc gactcgggcg gaaaggctag cgagcgcgac
     1681 gtctggccgc tcgcgctcta ctactggggc cgcacctgga cgatcggcgc ttggtgcgag
     1741 cggcgaacag acttccgcaa tttccgcgtg gaccggatcg cgggcctcga aacgctcgag
     1801 cgccgctatc cggacgaggc gggcaagcgg ctcgccgatt tcatccgcgc gatggaagcg
     1861 cggtgaagtg agccggctgt ccgaatgccg ccatcggaca ggtaccgggt gacggcatcc
     1921 ggcgccaggc gagccccggc gagcccgacg tggccgtccg gacgcagcaa atagaaagcc
     1981 tgccccggga tgcgcacgcg tgcgagctcc cgagcattgt gcgcatcgtc gggaatcacg
     2041 tgagtgctga gcaggtcgcc gagtcccggc gcttcgcccg gcgctgcggg ctgcccgaag
     2101 acgatcaggt tgtagcgcgt gtcgtcgagc ttctggaaca tgtcctcgac cggaccgttc
     2161 gcctggaacc tgagccgcaa ccacggaaaa cgatcgcccg cgcgcggcgc cc
//
LOCUS       DASPZD010000358         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_436959, whole genome shotgun sequence.
ACCESSION   DASPZD010000358 DASPZD010000000
VERSION     DASPZD010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436959"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VEM33_06940"
     CDS             complement(<1..617)
                     /locus_tag="VEM33_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYS51597.1"
                     /translation="MKAPIAAILAAFAAGLGLTGAAWAAEPCDIPAYLLFGNYELKHV
                     AEAVQKEKKLTIAVVGTGSSILAGPDGPRSAYPARLEAVLKQKLPSVAVKVVSLVRTR
                     MTTEDLARGMEKMLVDEKPDLVIWQTGTLDAIRRIDPDEFRAALEEGVETLHKGGADV
                     ILMNMQYSPRTDIMVPLGPYADNMRVVAQQHEIPLFDRLAIMRHWS"
     gene            complement(614..1783)
                     /locus_tag="VEM33_06945"
     CDS             complement(614..1783)
                     /locus_tag="VEM33_06945"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HYS51598.1"
                     /translation="MHALVVTTGRDLRLDLFRGIALWLIFLDHIPSNLVAWGTIRNYG
                     FSDATEIFVFVSGYTAAFVYGREMRERGFLIAGAHILRRAWQIYVAHVFLFAIYLAEI
                     SYVATSFENPLYAEEMNILDFLKQPEVTIVEALLLKFKPANMDILPLYIVLLLLFPPT
                     LWLLQFRSSLALGASVLLYVLTWTFNWNLPSYPSGHWYFNPFAWQLLFVFGAWCALGG
                     AQRLAAVLQSRVTLAIAVAYLLFAFVITLTWYLPYMSQYVPHWLEELIYPIDKVNLDV
                     LRFAHFLALAVVTVRFLPREWPGLKSRWAWPPIVCGQHSLEIFCLGVFLSFAAHFAMV
                     EVSGAIWMQFLLSALGIVVMIAAAGLLSWYKRAEGRSPGSRPKKTPDADLAGGEA"
     gene            1939..2466
                     /gene="apaG"
                     /locus_tag="VEM33_06950"
     CDS             1939..2466
                     /gene="apaG"
                     /locus_tag="VEM33_06950"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HYS51599.1"
                     /translation="MDAPKAAYRAVTRQIEVTATPRFLPERSSPENGYFFWAYTITLT
                     NRGAETVQLKTRHWRITDALGRLQEVRGAGVIGEEPVLKPGENFEYTSGVPLPTPSGF
                     MTGTYGMVTAAGEQFDIDIPAFSLDTPQRANDQLTRSHEVGRRHSAAPALMPTACAPP
                     RRRARTRKSSCRTRR"
     gene            complement(2393..>2933)
                     /locus_tag="VEM33_06955"
     CDS             complement(2393..>2933)
                     /locus_tag="VEM33_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HYS51600.1"
                     /translation="EHAAHEYFLRSEQIPTRVRLAVAEELRSGADGARHCWRAGGLLL
                     QFLPQSPERARRADLDPGDVPEGIEVKAAPEDDAWVEGRALVASVEDVELLDPNTSAE
                     RLLYRLFNERGVRVFRSRDLQAQCSCSRERVERMLRSFSQDDRDHMVEKGKITVTCEF
                     CNSTYVFEPDDVAERTPSA"
BASE COUNT          603 a          924 c          904 g          502 t
ORIGIN      
        1 gaccaatgcc gcataatggc aagccggtca aacagcggga tctcgtgctg ctgtgccacc
       61 acccgcatgt tgtccgcgta tgggccgagc ggaaccatga tgtcggtccg ggggctgtat
      121 tgcatattca tcaagataac gtcggcgccg cctttgtgca gcgtttcgac cccttcctcg
      181 agcgccgccc tgaactcatc ggggtcgatc ctgcggatcg cgtcgagagt acccgtttgc
      241 catatgacca ggtcgggctt ctcgtcgacg agcatttttt ccattccccg ggccaggtcc
      301 tcggtggtca tgcgcgttct gacgaggctg accaccttta ccgcgaccga aggaagcttc
      361 tgcttgagca cggcctcgag ccgcgccgga taggccgaac gcgggccgtc cggtccggca
      421 agaatggagg agccggttcc aaccacggcg atcgtgagtt ttttttcctt ctgcaccgcc
      481 tcggccacgt gcttcaactc atagttgcca aacagcaaat aggccgggat gtcgcagggc
      541 tccgcggccc acgcggcacc ggtgaggcca agcccggccg caaaggcggc caaaattgct
      601 gcaatgggcg ctttcatgcc tcgcctcctg cgaggtcggc gtccggcgtt ttcttgggac
      661 gcgatccggg actacgtcct tctgccctct tataccagct tagcaggccg gcagccgcaa
      721 tcatgacaac gatgcccaac gcgctgagca ggaattgcat ccagatggcg cccgacacct
      781 ccaccatggc gaaatgcgcg gcaaaggaca ggaacacgcc caggcagaag atttccagcg
      841 aatgctggcc gcagacaatc ggcggccaag cccagcgcga tttgagcccc ggccactcgc
      901 gcggcaggaa tctgaccgtc accaccgcca gcgcgaggaa atgcgcaaag cgcaggacgt
      961 cgagattgac cttgtcgatc gggtagatca actcctctag ccaatgcgga acgtactggc
     1021 tcatgtatgg cagataccag gtcagcgtga tgacgaacgc gaacagcagg taggccaccg
     1081 cgatcgccaa cgtcacgcgc gattgcagga cggccgcaag ccgttgcgct ccccccaatg
     1141 cgcaccaggc gccgaatacg aacagcaatt gccaggcgaa tggattgaag taccagtgcc
     1201 cgctgggata gctcggcagg ttccagttga aggtccatgt aagcacgtag agcagcaccg
     1261 aggcgcccag cgcgagcgag gatcgaaact gcagcaacca cagcgtcggt ggaaacaaca
     1321 gcagcagaac gatgtaaagc ggcaggatgt ccatattggc aggcttgaac ttgagcagca
     1381 gcgcctcgac gatcgtcacc tccggctgct tgagaaaatc gagaatgttc atctcttcgg
     1441 catagagggg attctcgaaa ctagtggcga cgtaggaaat ttcggcgaga taaatagcga
     1501 ataggaacac gtgggcgacg tagatttgcc aggctcgtcg caggatgtgg gcgcctgcga
     1561 tgagaaagcc gcgttcgcgc atttccctgc catagacgaa ggcggcggtg taaccggaga
     1621 cgaagacgaa aatttcagtc gcgtcgctga agccgtaatt acggatggtg ccccaagcga
     1681 ccaggttcga ggggatatgg tcgaggaaga tcagccagag cgcgattccg cggaacaaat
     1741 cgagccgcag atcgcgtccg gtggtgacca cgagggcatg cattccaggc gatctttcgc
     1801 gcaataaggg acacagctaa atctaatagg cctttgcccg gcttgggagt cacgtcatcg
     1861 cgacggttgc aggccgggcg ttgaagctgt tcactacggc cgcacatgcg acgaagcgcc
     1921 cggggtagag ggctgacaat ggatgccccc aaagcagcgt atcgggccgt gacccggcag
     1981 atcgaggtca ctgccacgcc ccgcttcctg cccgagcgat cctcgcccga gaacggctat
     2041 ttcttctggg cctataccat cacgctgacc aatcgcgggg ctgagaccgt gcaactgaag
     2101 acgcgtcatt ggcgcatcac cgacgcgctc ggtcggctgc aggaggtgcg cggggcaggc
     2161 gtcatcggcg aggagccggt cctcaagccc ggcgaaaatt tcgaatatac gagcggcgtg
     2221 ccgctgccca cgccctcggg tttcatgacc ggaacttacg gcatggtgac cgcggccggg
     2281 gagcaattcg acatcgacat tccggctttc tcgctcgaca ccccgcagcg agcgaacgat
     2341 caattgacgc gctcccacga ggtcggccgc cggcattctg ccgcgcccgc cctcatgccg
     2401 acggcgtgcg ctccgccacg tcgtcgggct cgaacacgta agtcgagttg cagaactcgc
     2461 aggtgacggt gatcttaccc ttctccacca tgtggtcgcg gtcatcctgg gaaaagctgc
     2521 gcagcatccg ttcgacccgc tcgcgcgaac acgagcactg cgcttgcaag tcgcggctgc
     2581 ggaagacccg cacgccgcgt tcgttgaaca gccggtagag caggcgctcg gcggacgtgt
     2641 tgggatcgag cagttcgaca tcctcgacgc tcgccaccag cgcgcggcct tcgacccagg
     2701 cgtcgtcctc gggcgcggcc ttaacctcaa tcccctccgg cacgtcgcct gggtcgagat
     2761 ctgcgcgccg cgcccgctcg ggcgactgcg gcaagaactg caacagcagc ccgccggccc
     2821 gccagcaatg gcgcgcaccg tctgcgcccg agcgcagctc ctcggcgaca gccaagcgca
     2881 ctcgcgtggg gatctgttcc gagcgaagaa aatactcgtg cgccgcgtgt tcg
//
LOCUS       DASPZD010000359         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_643612, whole genome shotgun sequence.
ACCESSION   DASPZD010000359 DASPZD010000000
VERSION     DASPZD010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643612"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VEM33_06960"
     CDS             complement(<1..339)
                     /locus_tag="VEM33_06960"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51601.1"
                     /translation="MSRIAAGLLALGMSLPMAGAFAQYPAKPIRVIVPWPAGGTVDGI
                     ARVIGPSLSSGLGRAIVVENKAGAGGSIGQAEAAKAAPDGHTALLVFDTHAVNHLLYK
                     NLGYDPFKSFE"
     gene            complement(336..1733)
                     /locus_tag="VEM33_06965"
     CDS             complement(336..1733)
                     /locus_tag="VEM33_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYS51602.1"
                     /translation="MSEISNPYTRGVAEFVSGLRYDAIPREVRERVKLLVLDSLGCAL
                     YGAHLQWCRILQRTLEKLDGSRGCRVWGTAQRLSAPHAALANGTQVQGFELDDVHREG
                     VLHVGAVVLPALVAVAELRGISGREFLTAAVAGYEVGPRVGLCMGPEHIGQGWHSGAT
                     LGVFAAGAGAARGLKLDADRTVHALGIAGTQAAGLMAAQYGAMVKRMHAGRSSQSGLY
                     GALLAEAGFTGIADVFESEYGGFCTTFSRSQDRFRLAELTAGFGEVWQTMGVALKFYS
                     CVGSNHTTLDALREMRREKAFDAKEVKKIVVHASQVTVDHAGWRYRPESLASAQMNLP
                     FCAATLLLEGDCFVGQFSERVVADPKRMALAEKIEVRPDPEISAKGAKSRHKVRVEVI
                     LDDGSRLERAVEVPRGSEPNFASEAQVVAKFERLAARALKRSRAAQLRDAVLGMEKLR
                     DAAQLAQLMTVGRRK"
     gene            complement(1730..2710)
                     /locus_tag="VEM33_06970"
     CDS             complement(1730..2710)
                     /locus_tag="VEM33_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51603.1"
                     /translation="MRSENSRDVLVIVAGLAASLLAAAALAQYPAKPIRLIVPFAAGG
                     GNDNVARLVGKRLSDSLGQPLVIDNRPGAGGALGAELAAKSAPDGYTLFLGGVGSHAV
                     NPNLNDSLPYDPIRDFAPVALLASAPLVLVVHPSVPAESFKAFVALARARPGQLNYAS
                     NGNGSSSHLAAVMFDSMTGVDMVHVPYKGLSPALTDLLSGRVQLMFSSVVAILPHVKS
                     EKLRGLAVTGNRRLTSMPDLPTIAESGLPGYEASSWYGVLAPAGTPREIVARLNAELV
                     KALEHPEVRTSLLAEGAEPIGGTPERFASHIRSEKERLGKLIREAKIRLE"
     gene            2725..3705
                     /locus_tag="VEM33_06975"
     CDS             2725..3705
                     /locus_tag="VEM33_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51604.1"
                     /translation="MAMFQAFTDKTMRCAFLSLLAALVLTDMASAQSWPSRPVRMIVP
                     QAAGGMADIVARVISSPLSRELGQQVIVDNRAGDGTLGAQAAARASADGYTYLFATAP
                     VLAINPYMAKILPYSPEDDFASVAMVGLSPFALIASPELNVNSLAQLIALAKANRDKI
                     VFASPGLRTLPGMLGEMLKIRAGISLLNVPYNSVPDTIAGRTHLAIQGIPAIAAVVQR
                     GQLRALAVTSHRRLPELPEVPTFSETLPGLEFNGWFAVVAPTGTPREAIQRMNLEFNR
                     ILLDGEMEQRLRALGLYTEGTGAPEEIDSFLKGERAFWSRVVRELKIEPE"
     gene            3747..5372
                     /locus_tag="VEM33_06980"
     CDS             3747..5372
                     /locus_tag="VEM33_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYS51605.1"
                     /translation="MGSVVWLKQSLADQASFGDAERNALARIFYVIAIVVFAALVLGT
                     FNNLFFDWPRFPFAIPAAAVILGALIAAARLGHLRVASILLPPVMLATVAHLLWTRDG
                     LHDNGVLSLAGVLVIGGVLLTRRAMIIFTIATLTVLLGVGYAEIAGLLQNRLSEFTDV
                     RHLVGVCLVLVVIAITARVIAESLFMSLRQAQEKSAALEESQGRLARMIEASPEAITI
                     GSLEDGKFIEANPACEQLTGYTREELIGRSPVELGFWPDPEERGRFISDLRRDQVVHG
                     RELRLRRKGGELRTILASAAIIDLKGRRFILFQAVDITERKSAEKALSEHEVMLRELS
                     AHHDSVREGERAHIAREIHDELGQALTALRMDLSVLGIKFGETDPQIREQVQELKGRV
                     DAIVQVVRDVATALRPAALDLGIVPGLEWLVEEFQKRNGIHCTVSVAESDIALGEDRS
                     IVLFRVVQESLTNVSRHSGARNVEILLDHDAERIRLDVEDDGVGFDVEAARSKKTFGL
                     LGIQERVIMLRGELSIASAPGRGTRVSVSMPIA"
     gene            5376..>5813
                     /locus_tag="VEM33_06985"
     CDS             5376..>5813
                     /locus_tag="VEM33_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYS51606.1"
                     /translation="MKTIRILVADDHAVVRRGLRHILDTTPDLAIAGEAAGGAEVMEK
                     LQAGEFDVLLMDVTMPATNPVELIARIKAQHPRVAVLIHSMHAESPVASRMLKAGASG
                     YITKDSEPEQLLAALRKVAAGGRYLGADLAEQLAFGNGAGKPLH"
BASE COUNT          954 a         1969 c         1940 g          950 t
ORIGIN      
        1 ctcgaaggac ttgaacgggt cgtagccgag gttcttgtag agcaggtgat tgacggcgtg
       61 cgtgtcgaac acgaggagcg cggtgtggcc gtcgggagcc gcctttgcgg cctccgcctg
      121 cccgatcgag ccgcccgccc ccgccttgtt ctcgacgacg atggcgcggc ccaggccgga
      181 ggacagactc ggtccgatga cgcgcgcgat gccgtccacc gtaccgcccg ccggccaggg
      241 aacgatcacg cggatcggct tcgccggata ttgagcgaac gcccccgcca tcggcagaga
      301 cattcccagc gcaagaagtc cggctgcgat tcggctcatt tcctcctccc gacggtcatc
      361 agctgcgcga gctgcgccgc gtcgcgcagc ttctccattc cgagcaccgc atcgcgcaat
      421 tgcgccgccc ggctccgctt gagcgcgcgg gcggccagcc tctcgaactt ggcgacgacc
      481 tgcgcctcgc tcgcgaaatt gggctcgctg ccgcgcggca cttcgaccgc gcgctccagg
      541 cggctgccgt cgtcgagaat cacctccact cgcaccttgt ggcggctttt cgcgcctttc
      601 gcgctgattt cagggtcggg ccggacttcg attttctcgg cgagggccat gcgcttcggg
      661 tccgcgacga ccctctcgct gaactgaccg acgaagcaat cgccctcgag aaggagcgtc
      721 gccgcgcaga aagggagatt catctgcgcc gaggcgaggc tttccggccg gtatctccag
      781 ccggcgtgat ccacggtcac ctgcgaagcg tgcacgacga ttttcttcac ttccttcgca
      841 tcgaacgctt tctcccgccg catctcgcgc agcgcgtcga gcgtggtgtg gttgctgccg
      901 acgcaggaat agaacttcag cgcgacgccc atcgtctgcc agacttcgcc gaagccggcg
      961 gtcagctcgg ccagtcggaa ccgatcctgg gagcgcgaaa aggtggtgca gaagccgccg
     1021 tactcgctct cgaacacgtc ggcgattccg gtgaagccgg cttccgcgag cagcgcgccg
     1081 tagagcccgc tctgcgagga gcgcccggcg tgcatgcgct tcaccattgc gccgtactgc
     1141 gccgccatca gccccgcggc ctgcgtgcct gcgatgccga gcgcgtgcac cgtcctgtcc
     1201 gcgtcgagct tcaagccgcg cgcggccccc gcgcccgccg cgaaaacgcc gagcgtcgcg
     1261 cccgagtgcc agccctggcc gatgtgctcg ggccccatgc acagccccac gcgcgggccg
     1321 acctcgtagc cggcgacggc cgcggtcaag aactcgcgtc cgcttattcc gcggagctcc
     1381 gcaacggcca cgagcgccgg cagcacgacc gcgccgacgt gcagcacgcc ttcacggtgc
     1441 acgtcgtcca gctcgaaccc ctggacctgc gtgccgtttg cgagcgccgc gtgcggcgcc
     1501 gacaggcgtt gcgccgtccc ccatacgcgg cacccgcggg agccgtccag cttctcgagg
     1561 gtgcgctgca ggatcctgca ccattgaagg tgcgcgccgt agagcgcgca gccgagcgaa
     1621 tcgaggacga ggagcttcac gcgctcgcgc acttcgcgcg gaatcgcgtc gtagcggagc
     1681 ccggaaacga attcggcgac gccgcgcgtg taggggttgg agatctcgct cattccaggc
     1741 ggatcttcgc ttcccggatc aatttgccga ggcgctcttt ttccgagcgg atgtgggagg
     1801 cgaaccgctc cggggtgcct ccgatcggtt ctgcgccctc cgcaagcagg ctcgtcctta
     1861 cttcggggtg ttcgagcgcc ttgaccagct cggcgttcag tcgcgcgacg atttcccgcg
     1921 gcgttcccgc cggcgcgagc acgccgtacc aggagctcgc ttcgtagccg ggcaggccgg
     1981 attccgcgat cgtcggaaga tccggcatcg aagtcagacg ccggttcccc gtcaccgcga
     2041 ggcctcgcaa tttttccgac ttcacgtggg gcaggatcgc aaccacgctg ctgaacatca
     2101 gctgcacccg gccgctcagc agatcggtga gcgccggcga aagtcccttg tagggcacgt
     2161 ggaccatgtc cacgcccgtc atggaatcga acatcaccgc ggcgaggtgg gacgagctgc
     2221 cgttgccgtt cgaggcgtag ttgagctgcc ccggccttgc gcgcgcgagc gcgacgaagg
     2281 ccttgaatga ctcggccgga acggagggat ggacgacgag cactagcggc gccgatgcga
     2341 gcagcgccac gggcgcgaag tcgcggatcg ggtcgtaggg aagactgtcg ttcagattcg
     2401 ggttgacggc gtggctcccc acgccgccga ggaacagcgt gtagccgtcg ggcgccgact
     2461 tcgccgcgag ctccgcgccc agcgcgcctc ccgccccggg ccggttgtcg atgacgagcg
     2521 gctggccaag gctgtcggac aggcgcttgc cgaccagacg cgcgacgttg tcgttcccgc
     2581 cgccggccgc aaacggaacg atcaggcgga tcggcttcgc cggatactga gcgagcgcag
     2641 ccgctgcaag gagcgaggct gcgagccctg cgacgatgac gagaacgtcc cgggaattct
     2701 ccgaccgcac ctcggaatgc tagcatggcg atgttccagg ccttcaccga caagaccatg
     2761 cgctgcgcat tcctttcgct gctcgccgct ctcgttctga cggatatggc gagcgcgcaa
     2821 agctggccct cgcggccggt gcggatgatc gtgccgcagg ccgcgggcgg gatggccgac
     2881 atcgtcgcgc gcgtcatttc cagcccgctg tcgcgcgagc tgggccagca ggtcatcgtc
     2941 gacaaccgcg cgggcgacgg aaccctaggc gcgcaggctg cggcgcgcgc gtccgccgac
     3001 ggctacacgt atttattcgc cacggccccg gtgctcgcca tcaacccgta catggcgaag
     3061 atccttccct acagcccgga ggacgatttc gcgagcgtcg cgatggtggg cctctccccg
     3121 ttcgcgctta tcgccagccc ggagctcaat gtgaattcgc tggcgcagct gatcgcgctc
     3181 gcaaaggcga accgcgacaa gatcgtcttc gcgagtcccg gcttgagaac gctccccgga
     3241 atgctcggcg aaatgctcaa gatccgcgca ggcatcagcc tgctgaacgt tccgtacaat
     3301 agcgtgccgg acaccatcgc cggccgcacc catctcgcca tccaggggat tcccgcgatc
     3361 gccgcggtcg tccagcgcgg gcagcttcgc gccctggcgg tgacctcgca tcgccgcctt
     3421 ccggagctgc cggaggtgcc gacgttttcc gagaccttgc cggggctcga attcaacggc
     3481 tggttcgcgg tggttgcgcc gaccggaacg ccgcgcgagg cgatccagcg catgaacctg
     3541 gaattcaacc gcatcctcct ggacggagag atggagcaac ggctccgggc gctcggcctg
     3601 tacaccgaag gcaccggcgc gcccgaggag atcgactcgt tcctgaaggg cgagcgcgcg
     3661 ttctggagcc gggtcgtgcg ggagctgaag atcgaaccgg agtagcgcga aagccccggg
     3721 ccgagcggcg aaacaccttg agggggatgg gatcggtcgt gtggctcaag cagtccctcg
     3781 ctgatcaggc gtctttcggc gacgccgagc ggaacgcgct tgcgcggatc ttctacgtca
     3841 tcgccatcgt ggtgttcgcg gcgctggtcc tagggacatt caataacctt ttcttcgact
     3901 ggccccgctt cccgttcgcc atacccgccg ccgcggtgat ccttggcgcg ttgatcgccg
     3961 ccgcgcgcct gggccacctt cgggtcgcca gcatattgct gccgccggtc atgctggcga
     4021 cggtggcgca ccttctgtgg acccgcgacg gcttgcacga caacggcgtc ctttcactcg
     4081 ccggggtgct cgtcatcggc ggcgtcctgc tgacgcggcg cgccatgata attttcacca
     4141 tagccacgct gacggtattg ctcggcgtcg gctacgccga gatcgccgga ttgctgcaga
     4201 accgtctgag cgaattcacc gacgtccggc atctggtcgg agtttgcctc gttctcgtcg
     4261 tcatcgcgat cacggcgcgc gtgatcgcgg aaagcctgtt catgagtctg cgccaggctc
     4321 aggaaaaaag cgccgcgctc gaagaatccc aggggcgact tgccaggatg atcgaggcaa
     4381 gcccggaagc gatcacgatc ggaagcctcg aggatggcaa gttcatcgag gccaacccgg
     4441 cgtgcgagca actgaccggc tacactcgcg aggagctgat cggccgcagc cccgtggagc
     4501 tgggcttctg gccggatccg gaagagcggg ggcgcttcat ttccgacctt cggcgggacc
     4561 aggtggtgca cgggcgggaa ctgcgcctgc ggcgcaaggg cggggaactg cggaccatac
     4621 tcgcttcggc ggcgatcatc gatctcaaag gccggaggtt catcctgttt caagcggtgg
     4681 acatcactga acggaagagc gcggagaagg ccctcagcga gcacgaggtg atgctgcggg
     4741 agctgtccgc ccatcacgac tcggtgcgcg aaggagagcg cgcccatatc gcgcgcgaaa
     4801 tccacgacga gctgggccag gccctgacgg cgctcaggat ggatctgtcc gttctcggca
     4861 ttaagttcgg cgaaaccgat ccccagatca gggagcaggt gcaggaactg aaggggcggg
     4921 tcgatgcgat cgtccaagtc gtgagggacg tcgccaccgc tctgcggccc gccgcgctcg
     4981 acctcggaat cgtccccgga ttggagtggc tcgtcgagga gttccagaag cgaaacggaa
     5041 tccattgcac ggtcagcgtc gcggaaagcg acatcgctct gggcgaggac cgcagcatcg
     5101 tgctgttccg cgtcgtgcag gagtcgctga cgaacgtctc tcggcactcc ggggcgcgca
     5161 acgtggagat cctgctcgat cacgacgccg agcgcatccg gctggacgtc gaggacgacg
     5221 gagtcgggtt cgacgtggaa gcggcgcgca gcaagaaaac cttcggcctc ctgggcatcc
     5281 aggagcgggt gatcatgctg cgcggggagc tgagcatcgc cagcgccccc gggcgcggca
     5341 cccgcgtctc cgtgtccatg ccgatcgctt agcccgtgaa aacgatcagg atactcgtgg
     5401 ccgacgacca tgccgtcgtg cggcggggct tgaggcacat actggacacg acgcccgatc
     5461 tcgccatcgc gggcgaggcg gcgggcggcg cagaggtcat ggagaagctg caggctggtg
     5521 agttcgacgt cctgctgatg gacgtcacga tgcccgccac caatcccgtc gagctgatcg
     5581 cgcgcatcaa ggcgcagcat cctcgcgtcg cggtgctcat ccacagcatg cacgcggaga
     5641 gccccgtcgc ttcgcggatg ctcaaagcgg gtgcttccgg ctacatcacc aaggacagcg
     5701 agcccgagca gctgctcgcg gccctccgaa aggtcgccgc gggaggaagg tacctcggcg
     5761 cggacctcgc cgaacagctc gccttcggca acggggccgg caagccgctg cac
//
LOCUS       DASPZD010000360         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1716564, whole genome shotgun sequence.
ACCESSION   DASPZD010000360 DASPZD010000000
VERSION     DASPZD010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716564"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VEM33_06990"
     CDS             <1..1023
                     /locus_tag="VEM33_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51607.1"
                     /translation="PNHWLLGWTIGVWVDEQDHVWVIHRGAGGLHDNEKGLELKPPIS
                     ECCRTAPPILVFDPAGNLVRSWGGPGPGYEWPEGAHGVHVDYKGNVWLGGNGKGDAHV
                     LKFTKEGKFLMQLGRYGKGAGSNDLENFGRVAKIWVDPKTNEAYIADGYANKRVAVVD
                     ADTGKMKRYWGAYGNKPDDAPMGNYDPKAPPAKQFRNPVHCVERSNDGLIYVCDRQAD
                     RVQVFQPDGKFVKEGFYAKETLASGSAWDIAFSKDRDQRFIFLADGQNEKVRIILRET
                     LEEISNFGDGGRQPGQFYGVHSIASDSKGNLYTTETYEGKRVQKFVYKGIGAVRAGEQ
                     GVLWPR"
     gene            1032..1961
                     /locus_tag="VEM33_06995"
     CDS             1032..1961
                     /locus_tag="VEM33_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51608.1"
                     /translation="MRCSLAGAVLLLASATALAQAFPSKPIRIVVPFPPGGGADTLAR
                     IMEPKLASIWAQPAIVENRPGASGHIGADLVAKSAPDGHTLLMSSTASLTEKNVDQFA
                     PVSLVSASPYVVTANPKVAAADIRGLIALAKASPGKLSFGSSGTGAASHLSAELFKSM
                     AGVDLLHVPYKGTGQALTDLLAGHVNLMFAPAQTVMPHVQSGKLKALGVTGARRSQTL
                     PDLPTVAESGLLGYEAVGWFGLLAPAATPKATVAKLSADANRVLAMRDVREKMLVLGA
                     EPAGNTPEEFARFIRGDQAKWSRLMKEAGITPE"
     gene            complement(1948..2424)
                     /locus_tag="VEM33_07000"
     CDS             complement(1948..2424)
                     /locus_tag="VEM33_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51609.1"
                     /translation="MQYRIGMERGFLRAELLDRETAEETRRFFRAVVFESVRHGCSRV
                     LVHVRSSKTLFTVERFGVLETFKKLASDPAHRIALLGDTVELGMSHDYVVLLARQQGI
                     TLRNFQNESQAIEWVRDRRQPEDRRHRSEQTMASVERRQSQRRKPPGDGSPDPTPA"
     gene            2706..>3818
                     /locus_tag="VEM33_07005"
     CDS             2706..>3818
                     /locus_tag="VEM33_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYS51610.1"
                     /translation="MNVVERFAGFAENYRRQPVALPVLHQAKRVVIDWYAALFPGSVV
                     PPATLLERSLAEEIGRGASRLADGRLAGVRAAALINGAAAHSVEVDDIYRDGIYHPGA
                     PTIAAALAVAQSRGATGESFLRAVIVGYEVSTRIAAAMGRAHYKYWHNTGTLGCFGAA
                     SAAAELLRLDARRFAHALATVATFSAGLQQAFRADSMSKPMHAGRAAEAGVTAALAAA
                     EGVTGSLDIMEGEVGYGRAMGEGPDWERALATLDEDFHITRMTFKNHACCGHAFAAID
                     GALALRARMGVAAGEIARVRVGSYRATLEVAGIADPATAAEARFSTPYLVATALTYGS
                     VRLAAFEPARLRDPATRSLMRRVEVVLDPELDAAFPA"
BASE COUNT          647 a         1250 c         1283 g          638 t
ORIGIN      
        1 ccgaatcatt ggctgctcgg ttggaccatc ggagtgtggg tcgacgagca ggaccacgtc
       61 tgggtcattc atcgcggcgc gggagggctg cacgacaacg aaaaggggct cgagctgaaa
      121 cctcccatct ccgagtgctg ccgcacggcc cctcccatac tggtgttcga cccggccggc
      181 aacctggtca gatcctgggg cggcccggga cccggctacg agtggccgga aggggcgcac
      241 ggcgtccacg tcgactacaa gggcaacgtg tggctcggcg gcaacggcaa gggcgacgcc
      301 cacgtcctca agttcaccaa ggaagggaag ttcctgatgc agctgggccg ctacggcaag
      361 ggcgcgggca gcaacgatct cgagaacttc gggcgcgtgg ccaagatctg ggtcgatccc
      421 aagaccaacg aagcgtacat cgccgacggc tacgccaaca aacgcgtcgc cgtggtcgac
      481 gccgataccg gcaagatgaa acgctactgg ggcgcgtacg gcaacaagcc cgacgacgct
      541 cccatgggca actacgaccc gaaggctccg ccggcgaagc agttccgcaa cccggtgcac
      601 tgcgtcgaac gctcgaacga cggtctgatc tacgtgtgcg accgccaggc cgatcgcgtg
      661 caggtattcc agccggacgg aaagttcgtg aaggaaggtt tctacgccaa agagacgctc
      721 gcctcgggct cggcctggga catcgcgttc tcgaaggacc gcgaccagcg gttcatcttc
      781 ctcgccgacg gccagaacga gaaggtgcgc atcatactgc gtgagacgct ggaggagatc
      841 tcgaacttcg gcgacggcgg gcgccagccc ggacagttct atggcgtgca cagcatcgct
      901 tccgattcca agggtaacct gtacacgacc gagacctacg aaggcaagcg cgtgcagaaa
      961 ttcgtctata aaggcatcgg cgcggtccgg gcaggcgagc agggcgtgct ctggcctcgc
     1021 tagaggagga gatgcgctgc tcgctcgccg gcgcggttct gcttctcgcg tcggcgacgg
     1081 ctctcgcgca ggcttttcct tcgaagccga tacgcatcgt ggttccgttc cctccgggcg
     1141 gcggtgcgga cacgctggcg cgcatcatgg agccgaagct cgcttcgatc tgggcgcagc
     1201 ccgcgatcgt cgagaatcgc ccgggcgcga gcggccatat cggcgcggat ctcgttgcca
     1261 agtccgcgcc cgacggccat acgctcctca tgagctcgac ggcatcgctt accgagaaga
     1321 acgtcgacca gttcgctccg gtttcgctgg tttcagcgtc gccctatgtc gtcaccgcca
     1381 atcccaaggt ggcggccgcc gacattcgcg ggctgatcgc gctcgcgaaa gcgagcccgg
     1441 ggaagctcag cttcgggtcc tcgggcacgg gcgccgcctc gcacctttcg gcggagctgt
     1501 tcaagtccat ggcgggcgta gacctgctgc acgtgcctta caagggcacc gggcaggcgc
     1561 tcaccgacct cctcgccggg cacgtgaacc tgatgttcgc gcccgcgcag acggtgatgc
     1621 ctcacgtgca gtcgggcaag ctgaaggcgc tcggcgtcac cggggcgagg cgctcgcaga
     1681 ccttgcccga tcttccgacc gtcgccgagt ccgggctgct gggctacgaa gccgtgggct
     1741 ggttcggcct gctcgcgccg gcggcgacgc cgaaagcgac ggtggcgaag ctctccgccg
     1801 atgcgaatcg cgtgctcgcg atgagggatg tgcgcgagaa aatgctggtc ctgggagccg
     1861 agcccgcggg caacaccccc gaggaattcg cgcgcttcat acgcggcgac caggcgaagt
     1921 ggtccaggct gatgaaagag gcggggatta cgccggagta gggtcgggtg agccgtcccc
     1981 cgggggcttg cggcgctggc tctggcgccg ctcaaccgac gccatggttt gttccgatcg
     2041 atgacggcga tcctccggct ggcgccggtc tctcacccat tcgatggctt gcgactcatt
     2101 ctgaaagttg cgaagagtga ttccctgctg acgcgcgagc aacacgacgt aatcgtgaga
     2161 catgcccagc tcgacggtat caccgaggag cgcgatcctg tgcgccggat ccgatgcgag
     2221 cttcttgaag gtctcgagga ccccgaatct ttcgaccgtg aacagcgttt tcgacgagcg
     2281 cacatgcacg aggacgcgtg agcagccgtg cctcacgctt tcgaagacca ctgcgcgaaa
     2341 aaaccttcgc gtttcttcag cggtttcccg atcgagcaat tccgccctga gaaaaccgcg
     2401 ttccatcccg atcctgtatt gcatagtctc tggggcgccc gtgcgactcc cctcatctcc
     2461 atccatcccc cccttgggcc gctgggcttc ggcccgtcac ttcaaacggc agcgcgtgaa
     2521 gtagtattat tcgttgacgc cgtccattgc ccgctcgaag gcggacgcgt tagtcgggaa
     2581 tccgacagat atagatacga tccgagttga attttaattc acgacctgcg ttgcaaaaac
     2641 gcgcgggaac cttggtctga atggaccatc ttctttcggc cgcatcgcgc tcaggaaaga
     2701 ctagagtgaa cgtcgtcgag cgtttcgccg ggttcgccga gaactatcgc aggcaaccgg
     2761 tggcgctgcc ggtgttgcac caggcgaagc gcgtcgtgat cgactggtac gcggcgctct
     2821 ttccggggtc ggtggttccg cccgcgaccc tgctcgaacg ctcgctcgcg gaagagatcg
     2881 gccggggcgc ttcgcgcctc gccgatggca ggctcgccgg ggtgcgtgcc gcggcgctca
     2941 tcaacggcgc ggcggcgcac agcgtcgagg tcgacgacat ctatcgcgac gggatctacc
     3001 acccgggggc gccgaccatc gccgcggcgc tcgcggtggc gcagtcgcga ggcgccacgg
     3061 gcgagtcctt cctgcgcgcg gtgatcgttg gttatgaggt gtcgacccgg atcgccgccg
     3121 ccatgggccg tgcgcactac aagtactggc acaacaccgg caccctaggc tgtttcggcg
     3181 cggcgagtgc cgccgccgag ctgctccgcc tcgacgcaag gcgcttcgcc catgcgctcg
     3241 ctacggtcgc cactttctcc gccggcctgc agcaggcgtt ccgcgcggat tcaatgtcca
     3301 agcctatgca cgcggggcgc gcagccgaag ccggcgtcac cgcggccctc gccgcagccg
     3361 aaggcgtcac cggatcgctc gacatcatgg aaggcgaggt gggctatggc cgggcgatgg
     3421 gtgagggacc ggattgggag cgcgcgctcg ccacgctgga cgaggacttc cacatcaccc
     3481 ggatgacgtt caagaaccac gcttgctgtg gtcacgcctt cgccgcgatc gacggcgcgc
     3541 tggccctgag ggcgcgcatg ggcgtggccg ccggggagat cgcgcgggtg cgcgtcggga
     3601 gctaccgcgc gactctggag gtggcgggga tcgcggaccc tgcgacggcc gccgaggcgc
     3661 ggttcagcac gccgtatctc gtcgcgactg cgctcacgta cggcagcgtg cggctcgccg
     3721 ctttcgagcc ggcgcggctc cgggacccgg cgacgcgctc gctgatgcgg cgcgtggaag
     3781 tagtcctcga cccggagctg gacgcggcgt ttccggcg
//
LOCUS       DASPZD010000361         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_491875, whole genome shotgun sequence.
ACCESSION   DASPZD010000361 DASPZD010000000
VERSION     DASPZD010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491875"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..715
                     /locus_tag="VEM33_07010"
     CDS             170..715
                     /locus_tag="VEM33_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HYS51611.1"
                     /translation="MPVGRNDPCPCGSGKKYKRCCGAVVRIKPVSATHAARQCGTCTA
                     CCDGWVKGTVFGHEMYPGQPCHFRGESCCSIYERRPVDPCRNFVCGWLQEDSPFPDEF
                     KPDRLGVLIIPVRWRTKTAYILRSAGRDPDEPLLAWMREFSLKTDRPFFYERSGERFG
                     FGPREFQIEMLAKLELGERLW"
     gene            784..2046
                     /locus_tag="VEM33_07015"
     CDS             784..2046
                     /locus_tag="VEM33_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HYS51612.1"
                     /translation="MQGNSVLSTLRRVSGALFIGLALTGTALAQQPLKIGFGMSLTGP
                     LAGNGKAALIAMEIWRDDVNAKDGILGRKVEFVYYDDQTSPATVPSIYAKLLDVDKVD
                     LVVSGYGTNVIAPAMPIIMQRNLTFMTLFGLNVNSKFNYDRYFQIMPAGPNPAVGWSQ
                     GFFDAAMSMNPKPKTVALVGADAEYPAMALEGAREHAKKTGLKIVYDKTYPPNTVDYS
                     PIVRAIQAANPEVVFVASYPPHSAGMVRAANEVGLKTRIFGGGLVGPQFAALKTQLGP
                     LLNGVVNYEFYVPDLGSKFPFLDQFLAKYQARAIVEKVDPLGFYLPPYAYAMMQVLEQ
                     AITATKGLDQAKLAEYIHKNTFDTYVGKVGFAKNGEWAAPRVMMTQYHGITGNDVEQF
                     KKPGRLTVLSPKEYKTGDLKAPYHENKK"
     gene            2050..>2484
                     /locus_tag="VEM33_07020"
     CDS             2050..>2484
                     /locus_tag="VEM33_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51613.1"
                     /translation="MNGAGGETRRRSFQRMEVSWALLANAVIAGLLLGGFYAAMSVGI
                     SISFGMLDVVNIAHPAFIILGSYIAYIVNDRFGYDPIAVSVVASPLFFLLGIVLYRIY
                     YVCFERRGQESLRGLAFFFGILFITEVALVLIFGVDYRMVSTR"
BASE COUNT          482 a          797 c          769 g          436 t
ORIGIN      
        1 ggcgagaacg ccgaccatga aggaattcgc gccgagatgc agggcgtcca gcgacaccag
       61 caccttgctg ccgcgatggg cgatgctcga aagcaccgac aataggatga cgaaggagac
      121 cgacatgacg ctcgtgcgcc aagtctagcg catgcgctag actcttccga tgcccgtcgg
      181 acggaacgat ccctgcccct gcggcagcgg aaaaaaatac aagaggtgct gcggcgcggt
      241 ggtacggatc aaaccggtct ccgcgacgca cgcagcgcgg cagtgcggca cctgcaccgc
      301 atgctgcgac ggctgggtca agggcacggt cttcggccac gaaatgtacc cgggacagcc
      361 ctgccacttc cgcggagaaa gctgctgcag catctacgag cgccggccgg tcgacccgtg
      421 ccgcaacttc gtctgcgggt ggctgcagga ggacagtccc tttccggacg aattcaagcc
      481 ggaccgcctg ggcgtgctga tcattccggt tcgatggaga acgaagaccg cctatatcct
      541 gcgctcggcg gggcgcgacc cggacgagcc gctgctcgca tggatgcgcg aattcagcct
      601 gaagaccgat cgcccgttct tctacgagcg ctcgggcgag cgcttcggtt tcgggccgag
      661 ggaattccag atcgagatgc ttgccaagct cgagctgggc gaacgcctgt ggtagagggt
      721 atcgcttgac aaatagcagg gcgctttcat aatcgccgga caggctaaac cgaggaggga
      781 accatgcaag gtaatagtgt tctgagcact ttaaggcgcg tgtcgggcgc cctgttcatc
      841 gggcttgcgc tcaccggcac ggcgctcgcg cagcagccgc tcaagatcgg cttcggcatg
      901 agcctcaccg ggccgctcgc gggcaacggc aaggcggcgc tgatcgcgat ggagatctgg
      961 cgcgacgacg tcaacgcgaa ggacggaatc ctcgggcgca aggtcgagtt cgtctactac
     1021 gacgatcaga ccagccccgc caccgtcccg agcatctatg ccaagctcct cgacgtggac
     1081 aaggtcgacc tcgtggtgtc gggctacggc accaacgtca tcgcgcccgc gatgccgatc
     1141 ataatgcagc gcaatctcac cttcatgacg ctgttcggcc tgaacgtcaa ctccaagttc
     1201 aactacgacc gctacttcca gatcatgccg gcgggtccga acccggcggt cggctggtcg
     1261 cagggcttct tcgacgcggc gatgagcatg aaccccaagc cgaagaccgt cgcgctggtg
     1321 ggcgcggacg cggaataccc ggcgatggcg ctcgagggcg cgcgcgagca cgccaagaag
     1381 acgggcctca agatcgtcta cgacaagacc tatccgccga acacggtgga ctactcgccg
     1441 atcgtgcgcg cgatccaggc ggccaacccc gaggtcgtct tcgtcgcttc ctatccgccg
     1501 cattcggcgg gcatggtgcg cgccgccaac gaagtgggcc tcaagacgcg gatattcggc
     1561 ggcggcctcg tgggaccgca gttcgccgcg ctcaagacgc agctcggacc tctcctgaac
     1621 ggcgtggtga actacgagtt ctacgtgccc gacctgggga gcaagtttcc attcctcgac
     1681 cagttcctcg ccaagtacca ggcccgcgcg atcgtcgaga aagtggatcc gctcggcttc
     1741 tacctgccgc cctacgccta cgcgatgatg caggtgctcg agcaggcgat cacggcgacc
     1801 aagggactcg accaggcgaa gctcgccgag tacatccaca agaacacgtt cgacacctat
     1861 gtcggcaagg tcggattcgc gaagaacggc gaatgggccg cgcctcgcgt gatgatgacc
     1921 cagtaccacg gcatcaccgg caacgacgtc gagcaattca agaaacccgg gcgtctcacc
     1981 gtgctttccc cgaaggaata caagaccggg gacctgaagg cgccttatca cgaaaataag
     2041 aaatagacat tgaacggcgc cggcggggaa acccgccggc gcagttttca gcgcatggaa
     2101 gtctcgtggg cgttgctcgc caacgccgtc attgccggcc tcctgctcgg cgggttctac
     2161 gccgcgatga gcgtcggcat ctcgatctcg ttcgggatgc tcgacgtggt caacatcgcg
     2221 catccggcct tcatcatcct cggctcttac atcgcctata tcgtcaacga ccgcttcgga
     2281 tatgatccca tcgccgtttc ggtggtggcg tcgcccctgt tcttcctcct ggggatcgtt
     2341 ctgtaccgga tctattacgt ctgtttcgag cgccgcggac aggagtcgct gcgcggtctc
     2401 gcgttcttct tcggcatcct gttcatcacc gaggtcgcgc tcgtcctgat cttcggcgtg
     2461 gattaccgca tggtgagcac gcgc
//
LOCUS       DASPZD010000362         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1639922, whole genome shotgun sequence.
ACCESSION   DASPZD010000362 DASPZD010000000
VERSION     DASPZD010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639922"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..673
                     /locus_tag="VEM33_07025"
     CDS             110..673
                     /locus_tag="VEM33_07025"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HYS51614.1"
                     /translation="MSRDKEPFLSRWSRRKLDSAKDPAAPLPVVREPKTPPAAVPAAA
                     RPPAETELPPVDTLKGLASEYKDFLRPGVDEKLRQSALKKLFHDPHFNVMDGLDTYID
                     DYSKPDPIPDEMLKALKQANRMIFPEEAAEKDREIDEAARKAVADAEAAPAAETPPPQ
                     VALDDERVAPEPESQPAEDSKPPAKPA"
     gene            735..>2407
                     /locus_tag="VEM33_07030"
     CDS             735..>2407
                     /locus_tag="VEM33_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYS51615.1"
                     /translation="MALDDKTLKLCNCNRTMALDAKALASALKLKQPIEIHTELCRKQ
                     VASFQGALKDEACVIACTQEAPLFSELADEAGSTSDIKFVNIREHAGWSKEGAKATPK
                     IAALLALADLPEPEPVTGVSYKSGGQLLIVGPAETAIPWAEKLAGGLDVNVLITSQRG
                     GELPAERRYAVWSGKVKSVKGYLGAFEVAWEQDNAIDLEVCTRCNACIYACPEAAIDF
                     SYQIDMDKCKSHRECVKACGAIHAIDFERAERARSERFDMVLDLAREPALKTAQLPQG
                     YLAPGSDPLEQALAAAQLAQLVGEFEKPRFFAYNEKICAHSRSSKIGCNQCIDVCSTA
                     AISPDGDRVKVDPHLCMGCGGCATVCPSGAMTYAYPRAPDFGARLKRLLSVYAQAGGR
                     DACLLLHNAVESRALIAKLARRGRGLPARVIPLEIHHAASIGMDALLAALAYGAGQVV
                     VLATPKEAAEYGDAVRKQMGFAQTIANALGYGGAHFRLLATDEHAALEKEIWALSPAV
                     SVSKPATYNLPPEKRATLDFALDHLVKQAPEPQEEIGLGSGAPYGQVLV"
BASE COUNT          448 a          784 c          814 g          361 t
ORIGIN      
        1 tcgaatggat ttcctggctc gccgagtacg tccagaagca ctacaagccc gaggtgaaga
       61 agaaacgcag ccgcccgcct tcgttcaaag gcgccaagcg agacgccgcg tgagcaggga
      121 caaggagccg ttcctgtcgc gctggtcgcg gcgcaagctc gattcggcga aggacccggc
      181 tgcgccgctg cctgtcgtgc gcgaacccaa gacgcccccg gccgctgtac ccgccgcggc
      241 gcgaccgcca gcagagacgg aattgccgcc ggtcgatacg ctgaaggggc tcgcctccga
      301 gtacaaggat ttcttgcgcc ctggagtaga cgagaagcta cgccagtccg cgctgaaaaa
      361 gctttttcac gatccgcatt tcaacgtgat ggacgggctc gacacgtaca tcgacgatta
      421 ttcgaagccc gacccgattc cagacgagat gctgaaggcg ctgaagcagg cgaaccggat
      481 gatcttcccc gaggaagcgg cggaaaagga cagggagatc gatgaagccg cgaggaaggc
      541 cgtcgcggac gcagaagccg cgccagcggc ggagacgccg ccaccgcaag ttgcgctgga
      601 cgacgagcgc gtggcgcccg agccggagtc tcaaccggcg gaggattcga agcctccggc
      661 aaaacccgcc tgatcgcgcg gtcgggtgcg cgttccctgc taatatggtc gggctttcat
      721 atgaagtctg attcatggcg ctcgacgaca agactctcaa gctctgcaac tgcaaccgga
      781 ccatggcgct ggacgccaag gcgctcgcat cggcgctcaa gctcaagcag ccgatcgaga
      841 tccacacgga gctgtgccgc aagcaggtcg cgagcttcca aggggcgctg aaggacgagg
      901 cctgcgtgat cgcgtgcacg caggaagcgc cgctgttctc ggagcttgcg gacgaggcgg
      961 gctccacctc ggacatcaag tttgtcaaca tccgcgaaca cgcaggctgg tcgaaggagg
     1021 gcgcaaaggc gacgcccaag atcgcggcgc tgcttgcgct tgccgatctg cccgagcccg
     1081 aaccggtgac cggcgtgtcc tacaagtccg gcggccagct gctgatcgtc ggcccggcgg
     1141 agacggcgat tccctgggcg gaaaagctcg ccggagggct cgacgtcaac gtcctgatca
     1201 ccagtcagcg agggggagag ctcccggcgg agcggcgtta cgcggtatgg tcgggcaagg
     1261 tgaaatcggt gaaaggctat ctgggcgcgt tcgaggtcgc ctgggagcag gacaacgcga
     1321 tcgatctgga agtctgcacg cgctgcaacg cctgcatcta cgcctgccct gaagcagcga
     1381 tcgatttctc ctaccagatt gacatggaca agtgcaagtc gcaccgcgag tgcgtgaagg
     1441 cctgcggggc gatccacgcc atcgacttcg agcgcgccga gcgcgcgcgc agcgagcgct
     1501 tcgacatggt gctcgatctc gcgcgcgagc cggcgctcaa gaccgcgcaa cttccccaag
     1561 ggtatctggc cccgggcagc gacccgctcg agcaggcgct cgcggcggca cagctcgccc
     1621 agctggtcgg ggagttcgag aagccgcgct ttttcgccta caacgagaag atctgtgcgc
     1681 acagccgctc ctcgaagatc ggctgcaacc agtgcatcga cgtgtgctcg actgccgcca
     1741 tttcgccgga cggcgaccgc gtcaaggtcg acccgcacct gtgcatgggc tgcggcggct
     1801 gcgcgaccgt gtgcccctcg ggcgcgatga cctacgcgta tccgcgcgcg ccggacttcg
     1861 gagcgcggct gaagcggctg ctttcggttt atgcgcaggc gggcggcagg gacgcctgcc
     1921 tcctgctgca taacgcggtt gagagccgcg cgctgatcgc caagctcgcg cgccgcgggc
     1981 gcggcctgcc cgcgcgcgtg attccgctcg agatccatca cgccgcttcg atcggcatgg
     2041 acgcgctgct cgccgcgctc gcgtacggcg cgggccaggt ggtggtgctc gcgacgccga
     2101 aggaagcggc cgaatacggc gacgcggtga gaaagcagat gggttttgcc cagaccattg
     2161 ccaacgcgct cggctacggc ggcgcgcact tccggcttct ggcgaccgac gagcacgcgg
     2221 cgctggaaaa ggagatctgg gcgctctcgc ccgcggtctc ggtttcaaag ccggccacat
     2281 acaacctccc gccggagaag cgcgccacgc tcgacttcgc cctcgatcac ctcgtgaagc
     2341 aggcgcccga gccgcaggag gagatcgggc tcggctcagg cgccccttac gggcaggtgc
     2401 tcgtgaa
//
LOCUS       DASPZD010000363         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_167333, whole genome shotgun sequence.
ACCESSION   DASPZD010000363 DASPZD010000000
VERSION     DASPZD010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167333"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VEM33_07035"
     CDS             complement(<1..190)
                     /locus_tag="VEM33_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYS51616.1"
                     /translation="MSSHEGRPDFKSPPAGCDCHFHVFGPVGRYPYNQADLRYAPPVA
                     PVEDFLALSKALGLERFVF"
     gene            complement(207..1217)
                     /locus_tag="VEM33_07040"
     CDS             complement(207..1217)
                     /locus_tag="VEM33_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51617.1"
                     /translation="MAGKLKLSMMIGDYEIVRALKDGAVKPKGIELLVAAYPGTRKIH
                     DSVASGEACDINEFNGGAYVVQKYGGRDDFTALPVFPHRRFRHGFITINRSKGIERPT
                     DLVDRRVACRTLGAAANYWMRGHLEQDYKVPHRAITWVIESEDDASLQAPADLRIERA
                     PRGKDVEAMLLAGEVDAMIAPNVTRMIGAGDPRVAHLFPNYREIEADYHRRTGLFPIM
                     HVTTIASKIVARHPWVVESLVLAFEEAKQLAYKRLADPRNVPLAWYRTYWEEERARLG
                     PDPWEYGLSELNKRNYGTLVGWVHEQVLSGARPKLEDLFPREAFQLRLPLPRMHEIDY
                     KF"
     gene            complement(1220..2236)
                     /locus_tag="VEM33_07045"
     CDS             complement(1220..2236)
                     /locus_tag="VEM33_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51618.1"
                     /translation="MGQSRPRFGREGGVRIAVKKRCCSAIAAWACLAVPLAAQPQGYP
                     SRPIRSIMTVAGGADIVARLVAQGLSGALGQPVVVETQSGAGGAIGAESVARAAPDGY
                     TIMLTSASALVMSRFLSKSARLDPLRDFTPITKAFETVALVVTSPALAVGSMSELIEY
                     AKRHPGRLSYGTSGIGTTHHLAGESIRLLTGIDWVHVPYKGGPPVITDLIAGEIQVGF
                     SILATAAPFVASGKIKILAVNGAKRYHVIGNVPTVTEQLPGFEPPPTWGGYFGPAGMP
                     RTIVARLHDEIVRILNSREVRAKAEGIGFPVATSTPEELTATIQRDIEYTARIVKAVG
                     IKPE"
     gene            complement(2194..3171)
                     /locus_tag="VEM33_07050"
     CDS             complement(2194..3171)
                     /locus_tag="VEM33_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51619.1"
                     /translation="MTRRCAALSLTATLAILAPHGAAAADAYPAKPVRFVVAFPPGGG
                     TDIIARSIAHKLAERIAQQVVVDNRPGAGGNIGTDMVAKSAPDGYTLLMGSAGPLAIN
                     ASLFGKMPFDPIKDLAPVTLAASTPNVLVVHPALRAATLKELIALAKARPGEINFASS
                     GHGTPAHLAGELFNLMAGVKMVHVPYKGAAPALADLLGGQVQIMFSTMPPALPHVRDG
                     KLRALAVTSAKRSPAAPELPTVDETALPGFEANTWHGVVVPAGTPGAIVARLNREIVA
                     ILHLPDVVERFSSQGAEALGSTPQEFAAYIKSETLKWAKVVRDSGAKAE"
     gene            complement(3168..3311)
                     /locus_tag="VEM33_07055"
     CDS             complement(3168..3311)
                     /locus_tag="VEM33_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51620.1"
                     /translation="MTRKEIGEILLHSAIYCGYPAALGGFRTAREYFAKHGDKKAAGK
                     RRK"
BASE COUNT          508 a         1094 c         1129 g          584 t
ORIGIN      
        1 cgaagacgaa gcgctccagg ccgagggctt tcgagagcgc gaggaaatcc tcgacgggcg
       61 ctaccggagg cgcataacgc aggtcggctt ggttataggg ataccgtcca accgggccga
      121 acacgtgaaa gtggcagtcg cagccggccg gaggcgattt gaaatcggga cgtccctcgt
      181 gcgacgacat gagttccccg tcgtcttcag aacttgtagt cgatttcgtg catgcgcggc
      241 agggggagcc ggagctgaaa ggcctccctg ggaaacagat cctcgagctt ggggcgcgcg
      301 ccgctcagca cttgctcgtg gacccatccg accagcgtgc cgtaattcct cttgttcagc
      361 tcggagagcc cgtattccca gggatcgggg ccgagccgcg cccgctcctc ttcccaatag
      421 gtgcggtacc aggcaagagg cacattgcgc ggatcggcga ggcgcttgta ggcgagctgc
      481 ttggcctcct cgaaggcgag cacgaggctt tccaccaccc agggatgccg cgcgacgatc
      541 ttcgacgcga tggtcgtcac gtgcatgatg ggaaagagcc cggtgcgccg gtggtagtcc
      601 gcctcgatct cccggtagtt cggaaagagg tgtgcgacgc gcgggtcgcc tgcgccgatc
      661 atgcgcgtca cgttcggcgc gatcatcgcg tccacctcgc ccgcgagcag catcgcctcg
      721 acgtccttgc cgcgcggtgc tcgctcgatt ctcaggtccg ctggagcctg cagcgatgcg
      781 tcgtcctcgc tttcgatcac ccaggtgatg gcgcggtgcg gcaccttgta gtcctgctcg
      841 aggtgcccgc gcatccagta attcgccgcc gcgccgagcg tgcggcaggc gacgcgccgg
      901 tcgacgaggt cggtcggcct ttcgatcccc ttcgagcggt tgatggtgat gaagccgtgg
      961 cggaagcgcc ggtgcggaaa aaccgggagc gcggtgaagt cgtcgcgccc gccgtacttc
     1021 tgaacgacgt aggcgccgcc gttgaactcg ttgatgtcgc aggcctcgcc gctcgcgaca
     1081 ctgtcgtgga tcttgcgcgt gccgggatag gccgcgacga ggagctcgat gcccttcggc
     1141 ttgaccgcgc cgtccttcag ggcgcggacg atctcgtagt cgccgatcat catcgagagc
     1201 ttcagctttc ccgccatggc tattcaggct tgatcccgac tgccttgacg atccttgccg
     1261 tgtattcgat atcgcgctgg atggtggcag tgagctcttc gggcgtgctc gtcgcaaccg
     1321 ggaagccgat gccctcggct ttcgcgcgta cctcgcgcga gttcaatata cgcacgattt
     1381 cgtcgtgcag gcgggcgacg atcgttcgcg gcattccggc cggcccgaag tagccgcccc
     1441 aggtcggggg cggctcgaag ccgggcagct gctcggtcac ggtcggcacg ttgccgatga
     1501 cgtggtagcg cttcgcaccg tttaccgcga ggatcttgat cttgccggaa gcgacgaaag
     1561 gggccgcggt ggcgagtatg ctgaatccca cctggatctc gcccgcgatg aggtcggtga
     1621 tcacgggcgg cccgcctttg tagggcacgt gcacccagtc gatgccggtg aggaggcgga
     1681 tcgattcgcc cgcgaggtga tgcgtcgtgc cgattcccga ggtgccatag gagagtcttc
     1741 ccggatggcg cttcgcgtac tcgatcaact cgctcatcga cccgaccgca agcgcggggc
     1801 tggtcacgac gagggccacc gtctcgaagg ccttggtgat cggcgtgaaa tccctgaggg
     1861 gatcgaggcg cgcgctcttc gacaggaacc ggctcatcac cagcgcgctc gccgaggtca
     1921 gcatgatggt gtagccgtcg ggcgcggcgc gcgccacgct ttccgcgccg atcgcgccgc
     1981 ccgcgccgct ctgggtctcg accacgacgg gctggccgag cgcgccgctc aggccttgcg
     2041 cgaccaggcg cgcgacgatg tccgcgcccc cggcgacggt catgatcgag cggatcggtc
     2101 gcgacggata gccttgaggc tgcgccgcaa gcggcacggc caggcaggcc catgcggcga
     2161 tcgccgaaca gcagcgcttc ttcactgcga tcctcactcc gccttcgcgc ccgaatcgcg
     2221 gacgactttg gcccacttca gcgtttccga cttgatgtag gcggcgaact cctggggcgt
     2281 actgccgagg gcttcggcgc cctggctcga aaaccgttcg accacgtcgg gcaaatgcag
     2341 gatcgccacg atctcgcggt tcagccgcgc gacgatcgcg cccggcgtgc ccgcgggcac
     2401 caccacgccg tgccaggtgt tcgcctcgaa tcccggcagg gcggtttcat cgaccgtcgg
     2461 cagttccggc gcggcaggcg agcgcttcgc gctcgtcaca gcgagagcgc ggagcttccc
     2521 gtccctcacg tgcgggagcg cgggcggcat ggtcgaaaac atgatctgca cctggccgcc
     2581 gagtaggtcg gcgagcgccg gagccgcccc cttgtacggc acgtgaacca tcttgactcc
     2641 cgccatcaag ttgaacagct cccctgccag gtgcgcgggc gttccgtgcc cggaagaggc
     2701 aaagttgatt tcgcccggtc tcgccttggc gagcgcgatc agctctttga gggtcgccgc
     2761 ccgaagcgcc ggatgaacga cgagcacgtt gggtgtcgac gccgcgagcg tcaccggcgc
     2821 gagatccttg atcggatcga agggcatctt gccgaagagg ctcgcgttga ttgcgagcgg
     2881 gcccgcagat cccatcagca gcgtgtagcc gtcgggcgcg ctctttgcga ccatgtcggt
     2941 gccgatgttg ccgcccgcgc ccggacggtt gtcgaccacg acctgctgcg cgatgcgctc
     3001 ggcgagctta tgcgcgatcg agcgggcgat gatgtcggtg ccgccgcccg gcgggaaggc
     3061 gacgacgaag cgcaccggct tggcggggta ggcgtccgcc gctgcggcgc cgtgcggtgc
     3121 cagtatcgca agggtcgccg tgagcgacag agcggcgcag cggcgcgtca cttccttctc
     3181 ttccccgccg ccttcttgtc gccgtgcttg gcgaaatatt cgcgcgcggt acggaatccg
     3241 cccagcgccg ccggataccc gcagtagatc gcggagtgca gcaggatctc gccgatctcc
     3301 ttcctcgtca ccccg
//
LOCUS       DASPZD010000364         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1082356, whole genome shotgun sequence.
ACCESSION   DASPZD010000364 DASPZD010000000
VERSION     DASPZD010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082356"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /gene="ygiD"
                     /locus_tag="VEM33_07060"
     CDS             complement(<1..560)
                     /gene="ygiD"
                     /locus_tag="VEM33_07060"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HYS51621.1"
                     /translation="MAKAHSRMPVVFFGHGSPMNTLERNKYTESWRAFGESIPRPKAI
                     LCVSAHWYTEGTAVTAMEKPKTIHDFYGFPQALFDVQYPAPGDPQLASRVRELLEPGE
                     VRLDESWGLDHGTWSVLKHAYPGAEIPVVQLSIDGTQPPQFHYEIGKRLAPLRDEGVL
                     VAGSGNVVHNLRLMKRAGGAPFDWAVR"
     gene            complement(568..1263)
                     /locus_tag="VEM33_07065"
     CDS             complement(568..1263)
                     /locus_tag="VEM33_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYS51622.1"
                     /translation="MIEIRKSEDRGLADHGWLRSYHTFSFADYYDPENMGFGHLKVIN
                     EDRIQPGTGFGTHGHRDMEIISYVLEGELAHKDNMGNGSTIVPGDVQRMTAGKGVLHS
                     EFNHAQDVTHFLQIWIEPSVTGIPPSYEQKHFDAASKRGRLRLVASPDGREGSVTIHQ
                     DAFLYAALLDGADRATHKLQPGRRAYLHVARGKLTANGQPIEAGDALKATNTAEITLE
                     KGEGAEVLLFDLA"
     gene            complement(1310..2431)
                     /locus_tag="VEM33_07070"
     CDS             complement(1310..2431)
                     /locus_tag="VEM33_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HYS51623.1"
                     /translation="MRTLLALLLLFAATFAHAHKPSDSYLAIRAEGANLSGQWDIALR
                     DLDFAIGLDDSGDGDITWGEVKAHQADIAAYALARLKLRSGDVDCPAHATEFLVDDHS
                     DGAYSVMRFAAACPREVTTLEITYSLFADLDPQHRGLLRLEHGVATRTAIFGPERATQ
                     SFELAKISALTQFVDYAREGVWHIWIGFDHILFLLSLLLPAVLVLEGGRWRAVARFPP
                     AFWDVFKIVTSFTVAHSITLSLAALGVISLPSRLVESAIATSVVLAALNNVFPVVHGR
                     RWMVAFAFGLIHGFGFASVLRDLGLPQGALLIALVGFNLGVEVGQLVIVSAFLPVAYV
                     LRASWFYQRLVFVGGSIAIAAVAGIWLVERAFNMKIISP"
     gene            complement(2428..>3241)
                     /locus_tag="VEM33_07075"
     CDS             complement(2428..>3241)
                     /locus_tag="VEM33_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51624.1"
                     /translation="TDLEAATREDPRNARAWSWRAAIHMVQADYAKAREDCLALQKVE
                     SELYAVGCTAYLDGTTGKAAAAYRALSAAFAKNADAPPEVKVWVLTRLAELSLRQGDL
                     KQAEEHFRAAYFEPINDQFLLAAYADFLLDQGRPDEVIPLLVDWVKSDILLLRLALAE
                     QALKAPKAREHIDSLRSRFDAAALRGDKLHQQEEARFNLYLLGNKEKALALAQENWKL
                     QREPRDARILLEAALAMKDPKAAQEALDWLERSGHEEPGLRRTAEKLKALKR"
BASE COUNT          573 a         1031 c         1040 g          597 t
ORIGIN      
        1 cgcaccgccc agtcgaacgg cgccccccct gccctcttca tgaggcgcag gttgtgcacg
       61 acgttgccgc tgccggcaac gagcactccc tcatctcgca gcggcgcaag ccgcttgccg
      121 atttcgtagt gaaattgcgg cggctgggtg ccatcgatgc tcagttggac gacgggaatc
      181 tctgcgcccg ggtacgcgtg cttcagaacg gaccaggtgc catggtcgag tccccatgac
      241 tcgtccagcc gcacttcccc gggctcgagc agctcgcgca ctctggatgc gagctggggg
      301 tcgcccggcg cagggtactg gacgtcgaac agggcttgcg ggaagccgta gaaatcgtgg
      361 atcgttttcg gcttttccat tgcggtcacc gcggttccct ccgtgtacca gtgcgccgaa
      421 acgcacagga tcgctttggg ccgcgggatg gactcgccga atgctcgcca ggactcggtg
      481 tacttgttgc gctcgagtgt gttcatcggg ctgccgtgcc cgaagaacac caccggcatt
      541 ctggagtggg ctttcgccat ctcgcgccta tgccaggtcg aacaacagca cttcggcgcc
      601 ttcgcccttt tcgagggtga tttcagccgt gttggtggct ttgagagcgt cgcccgcctc
      661 gatgggctgt ccgttcgcgg taagcttgcc gcgcgccacg tgcaaatagg cgcgccgacc
      721 gggttgaagc ttgtgggtcg cgcggtccgc gccatcgaga agcgcggcgt acagaaaggc
      781 gtcctggtgg atcgtcacgg atccctcgcg cccgtcgggc gaggctacca gccgcagccg
      841 cccgcgcttg gacgccgcgt cgaaatgctt ctgctcgtag ctcggcggaa tgccggtgac
      901 gctgggctcg atccagatct ggagaaagtg ggtcacgtcc tgggcgtgat tgaactcgct
      961 gtgcagcacg cccttacccg cggtcatgcg ctggacgtcc cccggcacga tggtcgagcc
     1021 gtttcccatg ttgtccttgt gcgcgagctc gccctcgagc acgtagctga tgatctccat
     1081 gtcgcggtgg ccgtgagtcc cgaaacccgt acccggctga attcggtcct cgttgatcac
     1141 cttgagatgg ccgaatccca tgttctcggg atcgtagtag tcggcgaagg aaaaggtgtg
     1201 atacgagcgc aaccagccat gatcggccag gccgcggtcc tcgcttttgc ggatttcgat
     1261 cattgcgggc ttccgtgtcg atcgaatatg gggttgcgcg cgcaattcat caagggctga
     1321 tgatcttcat gttgaacgca cgctcgacga gccagattcc ggcgaccgcg gcaatagcga
     1381 tggacccccc gacgaagacc aggcgctggt agaaccagct cgccctcagc acgtacgcga
     1441 ccggcaggaa cgcgctcacg atcacgagct gacctacctc cacgccgaga ttgaagccga
     1501 cgagcgcgat gagcagcgcg ccctgcggca gtccgaggtc tctgagaacg ctggcgaaac
     1561 cgaagccgtg gatcagcccg aaggcgaaag cgaccatcca gcgccggcca tgcacgacgg
     1621 gaaagacgtt gttcagcgcg gcgagcacga ccgaggtcgc aatcgccgat tccaccagcc
     1681 gcgagggcag cgaaatcacc cccagcgcag cgaggctcag cgtgatcgaa tgcgccacgg
     1741 tgaacgaagt gacgatcttg aagacatccc agaacgccgg aggaaaccgc gcgaccgccc
     1801 gccagcgtcc cccttcgagc acgaggacgg cgggcaggag cagcgacagc aggaacagga
     1861 tatggtcgaa gccgatccag atgtgccata cgccttcacg ggcgtaatcg acgaactggg
     1921 tgagcgccga gatcttcgcg agctcgaagc tttgggtcgc ccgttccggg ccgaaaatgg
     1981 cggtgcgcgt ggccacgccg tgttccagcc tcaacaatcc cctgtgctgc ggatcgaggt
     2041 cggcgaagag gctgtaggtg atttcgagcg tcgtcacctc ccggggacag gcggccgcaa
     2101 agcgcatcac ggaatatgcg ccgtcgctgt ggtcgtcgac gagaaattcg gtcgcatggg
     2161 cggggcagtc gacgtcccca gagcgcagct tcaatctcgc cagggcatag gccgcgatat
     2221 ccgcctgatg cgccttcacc tcgccccagg tgatatcgcc gtctccgctg tcgtccaggc
     2281 cgatcgcaaa atcgagatca cgcaaggcga tgtcccattg cccgctcagg ttcgcacctt
     2341 ctgcacggat cgcgaggtaa ctgtcgctcg gcttgtgcgc gtgcgcgaac gtggctgcaa
     2401 aaagaagcag caatgcgagg agcgttctca tcgcttgagc gccttcagct tctccgccgt
     2461 gcggcgcaaa cccggctcct catgccccga gcgctcgagc cagtcgagcg cctcctgcgc
     2521 ggccttcggg tctttcatgg cgagcgccgc ctcgagcagg atgcgtgcat cgcgcggctc
     2581 gcgctgcagc ttccaattct cctgtgccag cgcgagggcc ttttccttgt tgccgagaag
     2641 atagaggttg aagcgcgctt cctcctgctg gtgcaatttg tcgccgcgca aggccgcggc
     2701 gtcgaaccgg gatctgagcg aatcgatgtg ttctcgcgcc ttcggtgcct tgagcgcctg
     2761 ttcggcaaga gccaggcgaa ggagcaggat gtccgacttc acccagtcga caagcaacgg
     2821 aatgacttcg tcggggcgcc cttggtcgag cagaaaatct gcgtacgccg cgagcaagaa
     2881 ctggtcgttg attggctcaa agtacgctgc gcggaaatgc tcctcggcct gcttcaggtc
     2941 gccctgtcgc aacgacagct cggcaaggcg cgtcaacacc cagaccttga cttcgggcgg
     3001 agcgtcggcg ttcttggcaa aggctgcgga aagggcgcga tatgccgcgg cggccttgcc
     3061 cgtcgtgccg tcgaggtaag ccgtgcagcc gacggcgtag agctcggatt cgaccttctg
     3121 cagggccagg caatcttccc gagcctttgc gtaatccgcc tgcaccatgt ggatcgcggc
     3181 gcgccaggac caggcgcgcg cgtttctggg gtcttcgcgc gttgcagctt ccaagtccgt
     3241 c
//
LOCUS       DASPZD010000365         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_634143, whole genome shotgun sequence.
ACCESSION   DASPZD010000365 DASPZD010000000
VERSION     DASPZD010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634143"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /gene="dnaJ"
                     /locus_tag="VEM33_07080"
     CDS             complement(<1..1112)
                     /gene="dnaJ"
                     /locus_tag="VEM33_07080"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HYS51625.1"
                     /translation="MSKRDFYDILAVAKNASEEEIKKAYRKLAMKHHPDRNPDDHHAE
                     GRFKEAKEAYEVLSDSQKRAAYDRFGHAGVDPNVGAAAGAGMGGFAEAFGDIFGDIFG
                     SATRGGRAGGAFRGADLRYNMEISLEQAARGFNTDIRVPAWDHCETCGGSGARPGTKP
                     KVCGTCGGSGAVRMTQGFFSIQQTCPTCHGSGRVIPDPCTTCHGDGRVKRNKVLEVSI
                     PPGIDEGQRIRLGGKGEPGMNGGPAGDLYVEIRIKPHDVFQRDGDDLHCEVPVSVATA
                     TLGGELEVATLSEKVSIAIPEGTQSGKTFRLRGKGIKGVRSSYPGDLFCHVRVEIPVR
                     LTEKQKKLLRDLENSLKEGGAKHSPQARSFMERMKG"
     gene            complement(1202..3136)
                     /gene="dnaK"
                     /locus_tag="VEM33_07085"
     CDS             complement(1202..3136)
                     /gene="dnaK"
                     /locus_tag="VEM33_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HYS51626.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGGQPKVIENSEGARTTPSVVAYTE
                     DGEILVGAPAKRQAVTNAKNTIFAVKRLIGRRFEEKEVQKDISLMPYKIVKADNGDAW
                     VEVRGKKIAPQQVSAEILRKMKKTAEDYLGEDVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKKDERGDRKIAVYDLGGGTFDISIIEIADVDGEKQF
                     EVLSTNGDTFLGGEDFDQRIIDYVIGEFKKEQGVDLGKDVLALQRLKEAAEKAKIELS
                     SSQQTEISLPYITADQSGPKHLSMKLTRAKLEALVEDLIQKTVEPCRIAMKDAGVKMS
                     DINDVILVGGQTRMPKVQETVRELFGREPRKDVNADEAVAVGAAIQGAVLSGERKDVL
                     LLDVTPLSLGIETLGGVMTKMIQKNTTIPTKFAQVFSTAEDSQPAVTIKVFQGEREMA
                     SGNKLLGEFNLEGIPPSPRGLPQIEVTFDIDANGILHVSAKDKATGKENKIAIKANSG
                     LTEEEIQRMVRDAEANKAEDHRRFELAQARNVGDATVHQIRKALSEHGGKLDAAEKGG
                     IEAAVKELEDALKGDDKESIESRTKALMAASQKLGEKMYADTQAQAAESTQSASQSAE
                     PEKKPADDVVDADYREVKRG"
     gene            complement(3261..>3374)
                     /gene="grpE"
                     /locus_tag="VEM33_07090"
     CDS             complement(3261..>3374)
                     /gene="grpE"
                     /locus_tag="VEM33_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYS51627.1"
                     /translation="PNTVVTVYQKGYLINDRVLRPALVAVAKTPDAPDEAS"
BASE COUNT          473 a         1173 c         1027 g          701 t
ORIGIN      
        1 aagcccttca tccgctccat gaaggagcgt gcctggggcg agtgcttggc gccgccttcc
       61 ttcagcgagt tctccagatc gcgcagcagt ttcttctgct tctcggtcag ccgcaccggg
      121 atctcgaccc ggacatggca aaacaggtcg cccggatagc tcgagcgcac gcccttgatt
      181 cccttgccgc gcagacggaa cgtcttgccg ctctgggttc cctccggaat cgcgatgctg
      241 actttctcgc tcagcgttgc cacctccagc tcgccgccga gcgtcgccgt cgccaccgag
      301 accggcactt cgcaatgcag gtcgtcgcca tcgcgctgga acacgtcgtg gggcttgatc
      361 cggatctcca catacagatc gccggccggg cccccgttca tgcccggctc gcccttgccg
      421 ccgaggcgaa tgcgctgccc ctcgtcgata ccgggcggta tgctgacctc gagcaccttg
      481 ttgcgcttca cgcgcccgtc gccgtggcag gtggtgcacg ggtcgggaat cacgcgcccg
      541 ctgccgtgac aggtcgggca ggtctgctgg atcgagaaaa agccctgcgt catccggacc
      601 gcgccggagc cgccgcaggt gccgcagacc ttcggcttgg ttcccggccg cgcgccactg
      661 cctccgcagg tctcgcagtg atcccaggcc ggaacgcgga tatcggtgtt gaagccgcgg
      721 gccgcctgtt ccagcgagat ttccatgttg tagcgcagat ccgcgccgcg gaacgcgccg
      781 ccggcacgcc cgccccgggt cgcgcttccg aagatatcgc cgaagatgtc gccgaaggct
      841 tcggcaaagc cgcccatgcc ggcgccggcc gccgcaccca cgttcggatc cacgccggcg
      901 tgtccgaagc ggtcataggc agcgcgcttc tgggaatccg acaggacctc gtaggcctcc
      961 ttggcctcct tgaatctgcc ctcggcgtgg tggtcgtccg gattgcggtc cgggtgatgc
     1021 ttcatcgcca gcttgcgata cgccttcttg atctcttcct cggaggcgtt cttggccacg
     1081 gccaggatgt cgtagaaatc tcgtttggac ataagccgtc agactgcgac aaacaacggt
     1141 ccaccgcgcg gatcgatcga acccgcgggg cggaccgccc acaaaccctg cgcggcgatg
     1201 ttcagccccg cttcacttcc cgataatcgg cgtccaccac atcgtcggcc ggcttcttct
     1261 cgggctcggc cgattgcgag gcggactggg tcgactctgc agcctgcgcc tgcgtgtccg
     1321 catacatctt ctcgcccagc ttctgggaag cggccatcag cgccttggtc ctggactcga
     1381 tcgactcctt gtcgtcgcct ttcagggcgt cctcgagttc cttgaccgcg gcctcgattc
     1441 cgcccttctc ggccgcgtcc agcttgccgc cgtgctcgct caaggccttg cggatctggt
     1501 gcaccgtggc gtcgcccacg ttgcgagcct gtgccagctc gaagcggcga tggtcctcgg
     1561 ccttgttggc ctcggcatcg cgcaccatcc gctggatctc ctcctcggtc agacccgagt
     1621 tggccttgat cgcgatcttg ttttccttgc cggtcgcctt gtccttggcg ctcacgtgca
     1681 ggatcccgtt ggcgtcgatg tcgaacgtga cctcgatctg cggcagcccg cgtggagacg
     1741 gcggaatgcc ctccaggttg aactcgccca gcagcttgtt gccggaagcc atctcgcgtt
     1801 cgccctggaa caccttgatc gtgaccgccg gctggctgtc ctccgcggtc gagaagacct
     1861 gcgcgaactt ggtcgggatc gtggtgttct tctggatcat cttggtcatc acgccgccga
     1921 gcgtttcgat gcccagcgac agcggcgtca cgtccagcag cagcacgtcc ttgcgctcgc
     1981 ccgacagcac ggcgccctga atggcggcgc ccaccgcaac cgcctcgtcc gcattgacgt
     2041 ccttgcgcgg ctcgcgcccg aacagctcgc gcaccgtttc ctgcaccttg ggcatccggg
     2101 tctgtccgcc caccaggatc acgtcgttga tgtcggacat cttcacgccc gcatccttca
     2161 tcgcgatccg gcacggctcg accgtcttct ggatcagatc ctcgaccagc gcctccagct
     2221 tggcgcgcgt gagcttcatg ctcaggtgct tgggcccgct ctggtcggcc gtgatgtacg
     2281 gcagactgat ctcggtctgc tgcgacgagg acagctcgat cttggccttc tctgcggcct
     2341 ccttcaggcg ctgcagggcc agcacgtcct tgcccaggtc gacgccctgc tccttcttga
     2401 actcgccgat cacgtagtcg atgatccgct ggtcgaagtc ctcgccgccc aggaacgtgt
     2461 cgccgttggt cgacagcacc tcgaactgct tctcgccgtc cacgtcggcg atctcgatga
     2521 tcgagatgtc gaacgtgccg ccgcccaggt catagacggc gatcttgcgg tcgccccgct
     2581 cgtccttctt gtccaggccg aaagccagcg cggcggcggt cggctcgttg atgatccgct
     2641 tgacctccag gcccgcaata cgcccggcgt ccttggtcgc ctggcgctgc gagtcgttga
     2701 agtacgccgg aaccgtgatg accgcttcgg tcacgtcctc gccgagatag tcctcggcgg
     2761 tcttcttcat cttgcggagg atttccgcgg agacctgctg gggcgcaatc ttcttgccgc
     2821 gcacctcgac ccacgcgtcg ccgttgtcgg ccttgacgat cttgtacggc atcagggaga
     2881 tgtccttctg gacttccttc tcctcgaagc ggcggccgat cagccgcttg accgcgaaga
     2941 tcgtgttctt cgcgttggtg acggcttgcc gcttggccgg cgcgcctacc aggatttcgc
     3001 cgtcctccgt ataggcgacg accgagggcg tcgtgcgtgc gccctccgaa ttctcgatca
     3061 ctttcggctg gccgccttcc atgatcgcga cgcagctatt ggtggtgccg aggtcgattc
     3121 cgatgatctt ggccatgatg tgaaacctcc gtgttcttgg ggaatgcgta gctgtgcgct
     3181 aaatggggtt gggcagaaat tgaatcaacc ggtcgggcgg cgctgcggcg agccaggaat
     3241 gcgggccgct ctcgcccatg tcaggaggct tcgtccggcg cgtccggcgt cttcgccacg
     3301 gcgaccagcg ccggacgcag cacccggtcg ttgatcagat agcctttctg gtagacggtc
     3361 accacggtgt tagg
//
LOCUS       DASPZD010000366         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1274923, whole genome shotgun sequence.
ACCESSION   DASPZD010000366 DASPZD010000000
VERSION     DASPZD010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274923"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VEM33_07095"
     CDS             <1..226
                     /locus_tag="VEM33_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS51628.1"
                     /translation="FDRDTSPGAEPEDMLKLDIPALVIPGRDASHATSAARYLEECLP
                     RSEYWDVLPEGQTEQTAPARLLEFLQKVTL"
     gene            complement(233..847)
                     /locus_tag="VEM33_07100"
     CDS             complement(233..847)
                     /locus_tag="VEM33_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYS51629.1"
                     /translation="MKRLFIVTMGLLVIGFGFFGCSYMPWSDSGWTTLIDGSSGLDNF
                     NRVGDANWREEGGAIVADKGKGGYLVSKNSYKDFQIRAEFWADHTTNSGVFLRVTDPN
                     KITATDAYEVNIYDQRPEPEYGTGAIVNFAKVAPMPKAGGKWNTYEITAKGSQLTVVL
                     NGVQTVNIQNGKMAQGPFALQFGNGPKDAPGGAIKWRKVQIKTL"
     gene            complement(886..1797)
                     /locus_tag="VEM33_07105"
     CDS             complement(886..1797)
                     /locus_tag="VEM33_07105"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51630.1"
                     /translation="MTVKERTKPQVATMNAVHDLPVLVARDKGFFKDEGLDLEFVTTP
                     GMAQVTTSHFVKFDSVFDRPLDSVYNEGGIDQYRMCEWGIMKRAVEANTQGLRGRKIV
                     ALGASMSKFAIVVARGSKYYEPEMLKNQPIAVTPNNGSDFTTLKMMEGFLAPEHVKRT
                     NAGSMLKRLEAVRDGKVAAASLMEPWISVAQKWGLRVLIESHSTRSEAAGDDLDGPTL
                     KKMFNAQARAVELIEKDPTPFIHYFIRETGGLLEPHEFQAWRLLHAAPQPYTRERFDD
                     TYNWTVKWNMTVPGATFENTVDNRAWN"
     gene            complement(1902..2198)
                     /locus_tag="VEM33_07110"
     CDS             complement(1902..2198)
                     /locus_tag="VEM33_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51631.1"
                     /translation="MFASVRKAPERLQAVDRVEQWTRERFGLPKEAAVSVAEVACTLP
                     GCAPLETVVMFWIAEQRYQFKLFKPVAEVVAGDLPYAWLKDALAVEEGAGWECC"
BASE COUNT          390 a          728 c          697 g          457 t
ORIGIN      
        1 cttcgaccgc gacacctctc ccggcgccga gcccgaagac atgctcaaac tcgacatccc
       61 ggcgctcgtc ataccgggac gcgatgcgtc tcacgcgacc tcggcagcga ggtatctcga
      121 agaatgcctt ccgcgctcgg aatactggga cgttctgccg gagggacaaa cagagcaaac
      181 cgccccagcg cggctgctgg aattcctgca gaaagtaacg ctgtagatca gactacaacg
      241 tcttgatctg caccttgcgc catttgatgg cgccgccggg ggcgtccttg gggccgttgc
      301 caaactgcag ggcgaacggt ccctgcgcca ttttgccgtt ctgaatattg acggtctgga
      361 caccgttcag cacgacggtg agctgcgagc ccttggccgt gatctcgtag gtgttccact
      421 tgccgccagc cttgggcatc ggcgctacct tcgcgaaatt cacgattgcg cccgtgccat
      481 attcaggttc ggggcgctgg tcgtagatat tcacctcgta ggcgtcggta gcggtgatct
      541 tgttgggatc ggtaacgcgg aggaaaacgc cgctgttcgt ggtgtgatcc gcccagaact
      601 ccgccctgat ctggaagtct ttgtaggagt tcttcgatac aagatagcca cccttgccct
      661 tgtcggcgac gatggcgccg ccctcctctc tccagttggc gtcgccgacg cggttgaagt
      721 tgtccaggcc ggaggagccg tcgataagcg tggtccagcc cgaatcggac cacggcatgt
      781 atgaacagcc gaaaaagccg aatccgatga cgagcagccc catcgtgacg ataaacaggc
      841 gtttcatcgc gacctcctcc ttgagttgat gacgattgtt tcccgctagt tccaggcgcg
      901 gttatccacc gtgttctcga aagtcgcgcc gggcaccgtc atgttccact tcaccgtcca
      961 gttgtaggtg tcgtcgaagc gctcgcgcgt gtagggctgc ggcgcggcgt gcagcaggcg
     1021 ccaggcctgg aattcatggg gttcgagcag gccgccggtc tcgcggatga aatagtggat
     1081 gaagggcgtc gggtcttttt cgatgagctc gaccgccctc gcctgcgcgt tgaacatctt
     1141 cttgagcgtc gggccgtcga ggtcgtcgcc cgccgcctcg ctgcgcgtcg agtgcgactc
     1201 gatcaggacg cgcagacccc atttctgcgc cacgctgatc cagggctcca tcaggctcgc
     1261 cgcagcgacc ttgccatcgc gcaccgcttc gagccgcttg agcatcgacc ccgcgttggt
     1321 cctcttgacg tgctctggcg caaggaatcc ctccatcatc ttcagcgtgg tgaagtccga
     1381 gccgttgttc ggggtcaccg cgatcggctg gttcttcagc atctcgggct cgtaatactt
     1441 cgagccgcgc gcgacgacga tggcgaactt cgacatggag gcgccgagag ccacgatctt
     1501 gcgcccgcgc agaccctgcg tgttggcttc gacggcgcgc ttcatgatgc cccattcgca
     1561 catgcggtac tggtcgatgc cgccttcgtt gtacaccgag tcgagcgggc ggtcgaagac
     1621 ggaatcgaac ttgacgaagt gggaggtcgt gacctgtgcc atgcccggcg tagtcacgaa
     1681 ctcgaggtcc aggccctcgt ccttgaagaa gcccttgtcg cgggccacga ggacgggcag
     1741 gtcgtgcacc gcgttcatcg tcgctacctg gggcttggtt ctttccttga cggtcatgat
     1801 gcgttctcct atgtggaccg agactccctc ggtctcgtgt cgcgatgcgt cgatggcgat
     1861 gatccctcta catccgctcg gctgtcaacg gcgagagggc ctcagcagca ctcccagccc
     1921 gccccctcct cgaccgcgag cgcgtccttc agccaggcgt agggaagatc gccggcgacg
     1981 acctccgcca ccggcttgaa cagcttgaac tggtagcgct gttccgcgat ccagaacatg
     2041 actacggtct cgagcggcgc acagccgggg agcgtacagg cgacctccgc aacggagacc
     2101 gccgcctctt tcggaaggcc gaagcgctcg cgcgtccatt gctccacgcg gtcgacggcc
     2161 tggaggcgtt cgggagcttt cctgacggag gcgaacacgg cgtcagccta tcagaagcgg
     2221 cgttgcatga gaaaatgcgc cgatggcgca ccccgaccgc ttcctcctcg ca
//
LOCUS       DASPZD010000367         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_875568, whole genome shotgun sequence.
ACCESSION   DASPZD010000367 DASPZD010000000
VERSION     DASPZD010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875568"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VEM33_07115"
     CDS             <1..343
                     /locus_tag="VEM33_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS51632.1"
                     /translation="MSDVSPSESGLASGVVNTSFMMGGALGLAVLASLAAARTDRLLS
                     FGADSLAALNGGYHIAFAIGAVFAAAAALLSAGLLRSGQQAGAHGRDKAAPGSAGEMQ
                     AAAGPAGKSTG"
     gene            371..1045
                     /locus_tag="VEM33_07120"
     CDS             371..1045
                     /locus_tag="VEM33_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYS51633.1"
                     /translation="MTLQTETWTHELGRRVRTLLVLKMLANLSGIAAFFYAYFWIMRH
                     PLSAATVIPVIWIDDLVPFSPQSFFLYASLWVYVALGSLFTKDGRELAAWAAACFSMI
                     IVGLGIFMALPTKVPDFAVDWSQYPSLEFLKAVDASGNALPSLHAAFAVFTGVVLHGQ
                     LTAVRAPRPVLACNVLWCLGIVYSAMATRQHLALDIIAGAVLAGVVSIAYVTAGRAPH
                     TAQEES"
     gene            1042..1578
                     /locus_tag="VEM33_07125"
     CDS             1042..1578
                     /locus_tag="VEM33_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYS51634.1"
                     /translation="MIKKIGIAVVVLLGALLIYAATQPDTFRIQRAASIKAPPERIFA
                     VLNDFLRWESWSPWEKKDPAMKRTFSAVTSGKGAVYAWEGNKDVGQGRMEIAESLPPS
                     KVAIKLDFVKPFETHNIVEFTLEPQGDSTNVTWVMQGPMLYISKLITVFVSMDSMVGK
                     DFEAGFANLKTVTENQPR"
     gene            1598..1765
                     /locus_tag="VEM33_07130"
     CDS             1598..1765
                     /locus_tag="VEM33_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51635.1"
                     /translation="MQKIRYGLPWQVNPAILGELLSGPDVKKAKRVMEAMLKMKKIDI
                     AALKAAAEAAS"
     gene            1762..>2036
                     /locus_tag="VEM33_07135"
     CDS             1762..>2036
                     /locus_tag="VEM33_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS51636.1"
                     /translation="MKEPRYGWVMVALGAFMTCIAIGAMFSLAVFLRPITESTGWSTT
                     AVSSAMTLNFLAMGAAGFGWGALSDRIGPRPVVLAGAVLLGSGLFLA"
BASE COUNT          356 a          645 c          648 g          387 t
ORIGIN      
        1 catgagcgac gtgagcccga gcgaatccgg gctcgcctcc ggagtcgtca acacctcgtt
       61 catgatgggc ggagcgctcg gcctcgcggt cctcgccagc ctcgccgccg cgcgcacgga
      121 caggctgctc tcgttcggag cggattcgct cgccgcgctc aacggcgggt accatatcgc
      181 cttcgccatc ggcgccgtct tcgcggccgc ggcggccctg ctcagtgcgg ggctgcttcg
      241 ctcggggcag caggcgggcg cgcatggacg cgataaggcg gctcccggct ccgccggaga
      301 aatgcaagcc gcggcgggac ccgccggtaa atccaccggc tgaccgcggg gtcgtgaaga
      361 caaggccacg atgacgcttc aaacggagac ttggacgcac gagctgggca ggcgggtccg
      421 cactctgctg gtgctcaaga tgctcgcgaa cttgagcggg atcgcggcct tcttctacgc
      481 gtacttctgg atcatgcgcc atccgctttc cgcggcgacg gtcattcccg tgatttggat
      541 cgacgatctg gttccctttt cccctcagtc cttcttcctg tacgcgtccc tgtgggttta
      601 tgtcgccttg ggatctttgt tcaccaagga cggacgggag ctggctgcct gggcagcggc
      661 atgcttttcc atgatcatcg tcggcctggg catattcatg gctctgccaa cgaaggtccc
      721 cgatttcgcg gtcgactggt cccagtaccc gtcgctcgaa tttctcaagg cggtcgacgc
      781 ttcagggaat gccttgcctt cgctgcacgc cgccttcgct gtgttcacgg gggtcgtgct
      841 ccacggacaa ttgacggcgg tccgcgctcc gcggcccgtg ctcgcgtgca atgtgctgtg
      901 gtgtctcggc atcgtctact ccgcaatggc cactcgacaa catcttgcac tggatatcat
      961 cgcaggtgca gttctggcag gggtggtgtc gatcgcctac gtgaccgcag gccgtgcacc
     1021 ccatacagca caggaggaat catgatcaag aaaataggca ttgcagtcgt cgttctgctc
     1081 ggcgctctgc tcatctatgc cgcgacgcag cccgacacgt tccggatcca gcgcgcggcg
     1141 agcatcaagg cgccgccgga gaggatcttt gccgtgctca acgattttct gcgctgggaa
     1201 tcctggtcgc cctgggagaa gaaagacccc gcgatgaaaa ggaccttcag cgccgtaacc
     1261 agcggcaagg gcgccgtgta cgcgtgggaa ggcaacaagg acgtcggcca agggcgcatg
     1321 gagatcgcgg agtccttgcc gccatcgaag gtcgcgatca agctggactt cgtcaagccg
     1381 ttcgaaacgc acaacatcgt cgaattcacg ctcgaaccac agggcgactc cacaaatgtc
     1441 acctgggtca tgcagggccc catgctctat atttcgaagc tcattaccgt attcgtcagc
     1501 atggacagca tggtcggcaa ggacttcgag gccgggtttg ccaacctgaa gactgttacc
     1561 gagaaccaac cccgttagac aggagggagc aagagccatg caaaagatca ggtacggcct
     1621 gccgtggcag gtgaatcccg ccatcctcgg cgagctgctg agcggtcccg acgttaaaaa
     1681 ggcgaagcgc gtcatggagg cgatgctcaa gatgaagaag atcgacatcg cggccctgaa
     1741 ggccgcggcg gaggcggcgt catgaaagag ccacgttacg gatgggtgat ggtggcgctc
     1801 ggcgccttca tgacctgcat cgccatcggc gcgatgttct cgctcgcggt gttcctgcgg
     1861 ccgatcaccg agtcgaccgg ctggtcgacg acagccgtgt cgagcgccat gacgctcaac
     1921 ttcctcgcga tgggcgcggc cggcttcggg tggggcgcgc tcagcgaccg catcggcccg
     1981 cgacccgtgg tcctcgcggg cgcggtcctc ctgggctcag gcctcttcct cgcgag
//
LOCUS       DASPZD010000368         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1166035, whole genome shotgun sequence.
ACCESSION   DASPZD010000368 DASPZD010000000
VERSION     DASPZD010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166035"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..636)
                     /locus_tag="VEM33_07140"
     CDS             complement(160..636)
                     /locus_tag="VEM33_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HYS51637.1"
                     /translation="MALDLTILVGTMTGTAQLVAQELELTLDDGEIRVRTAPMDGLDA
                     GVFSGGGLFLICSSTYGQGDVPDNAKNLYESLQTARPDLSNVQYGVIALGDRTYAETF
                     CNGGKRFDAILSELGARRIGEIMLHDASAGTMPEEVAVTWVAGWISLCRKEARLTA"
     gene            723..1634
                     /locus_tag="VEM33_07145"
     CDS             723..1634
                     /locus_tag="VEM33_07145"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYS51638.1"
                     /translation="MSTTSIHVKKHSQQSVHTKESSASERDYLAALGERVREARAKRG
                     MSRKLLAGDSGVSERYLAQLEGGQGNVSILLLRQIAAALNIALAELLAEDPDEVVELT
                     LTTQFLKRLPRQTLAAVRSQLVRDYGSARDERMKRIALIGLRGAGKSTLGAKLARALG
                     APFVELDREIEREAGTGLSEIFLLYGQAGYRRYERRCLERVLEKNERAVIATGGSIVS
                     EPGTYELLLSACFTVWLKAEPEEHMARVIAQGDTRPMAGNDQAMEDLRRILDGRAVLY
                     GQADATVDTARKTPEQSLSALRKAVAP"
     gene            1651..>2375
                     /locus_tag="VEM33_07150"
     CDS             1651..>2375
                     /locus_tag="VEM33_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYS51639.1"
                     /translation="MSAVPETKPSPPLVTYDAQPDRYLHWRLSFDGPVATLAMDVNED
                     KALKPGYKLKLNSYDLGVDIELADALNRIRFEHPEAKCVVITSARERMFCSGANIYML
                     GQSTHAWKVNFCKFTNETRNGIEDSSRHEGLKSLAAVNGTIAGGGYELALACDEILMI
                     DDRSSTVSLPELPLLGVLPGTGGLTRVTDKRKVRRDLADVFCTTSEGVRADRAKQWKL
                     VDQTAKPQAFKDAVRERAAALSAL"
BASE COUNT          443 a          769 c          760 g          403 t
ORIGIN      
        1 aggaacgcaa gcttgctcgc gcggcctgca gagagattgc gctcgatcaa atcgtacgcg
       61 gcgttgtagt cccggggaat atggatttta ggcgggctga ccgagtggtc ggcggcgctg
      121 agcgacggca cgatcttctc actccccttt tcgatcgcct caggcggtga gtcgggcttc
      181 cttgcggcac agcgagatcc agccggccac ccatgtcacc gcgacttctt cgggcatagt
      241 gcccgcgctg gcgtcgtgca gcatgatctc gccgatgcgc ctggctccca gctccgagag
      301 aatcgcgtcg aaacgcttgc cgccgttgca gaaggtttcg gcgtaagtcc ggtcacccag
      361 cgcgatcact ccgtactgca cgttggagag gtcgggacgc gccgtctgaa gcgactcgta
      421 caagttcttc gcgttgtcgg gcacgtcgcc ctggccgtag gtggacgagc agatcaggaa
      481 caggccgccg ccggaaaaca cgcctgcgtc gaggccgtcc ataggcgcgg tccgcacgcg
      541 tatttcgcca tcgtcgaggg tgagctcgag ctcctgtgcg accagctgcg cggtgccggt
      601 catcgtcccc acgaggatgg tcaggtcgag cgccatcggc ttttttctcg ttcccgcttg
      661 cgtttcagta tcgagtgcat catactgcat gccgcgcgca tagcaagcat tatattgcat
      721 aaatgagcac aacgagtatt cacgttaaga aacactcgca acaatctgtt catacgaaag
      781 aaagcagtgc aagcgagcgt gactatctcg ccgctctggg tgagcgggtg cgggaagccc
      841 gcgcaaagcg aggcatgtca cgcaagctgc ttgccggtga ttcgggagtg tcggagcgct
      901 acctcgccca gctcgaaggc ggacagggca acgtttcgat ccttctgctg cggcagattg
      961 cagcagctct caatatcgcg ctcgccgagt tgcttgccga ggaccccgac gaggtcgtgg
     1021 aactcactct cacgacccaa ttcctgaagc gcctgccgcg gcagacactg gccgcggtgc
     1081 gctcgcagct tgtccgcgac tacggcagcg cgcgcgacga gcgcatgaag cggatcgcgc
     1141 tcatcgggct gcgcggcgcg ggcaaatcca cgctgggtgc gaagctcgcc cgcgcgctgg
     1201 gcgccccctt tgtcgagctc gaccgcgaga tcgagcgcga ggcaggcacc ggtctctcgg
     1261 agatttttct gctctacggg caagccggat accggcggta cgagcggcgc tgcctcgaga
     1321 gggtgctcga aaagaacgag cgcgccgtga tcgccacggg cggaagcatc gtttccgaac
     1381 ccggaaccta cgaactcctg ctctccgcct gcttcacggt gtggctcaag gccgagcccg
     1441 aggagcatat ggcgcgcgtg atcgcgcagg gtgacacccg ccccatggcc gggaacgatc
     1501 aggcgatgga ggatctgcgg cgtatactcg acgggcgggc cgtgctctac ggacaggccg
     1561 acgccacggt ggacacggcc cgaaagacgc cggagcagag tctctcggcg ttgcgcaagg
     1621 cagtcgcccc ctagcaccag gagccactcc atgagtgccg tgcctgaaac caaaccctcc
     1681 cctccccttg tcacctatga cgcgcagccg gaccgctatc ttcactggcg gctttccttc
     1741 gacgggcccg tcgcgacgct cgcgatggac gtgaacgagg acaaggctct caagccggga
     1801 tacaagctca agctcaactc ctacgacctc ggagtggaca tcgagcttgc ggatgcgttg
     1861 aaccgcatcc gcttcgagca ccccgaggcg aagtgcgtgg tgatcaccag cgcgcgcgag
     1921 cgcatgttct gctcgggcgc caacatctac atgctcgggc aatccaccca cgcgtggaag
     1981 gtgaatttct gcaagttcac caacgaaacg cgcaacggca tcgaggactc gagccgccac
     2041 gagggcttga agtccctcgc ggcggtcaac ggcacgatcg cgggcggcgg ctacgagctc
     2101 gcgctcgcct gcgacgagat cctcatgatc gacgatcgct cctcgacggt gagcctgccc
     2161 gagctccccc tgctgggcgt gctccccgga acgggcggcc tcacgcgcgt caccgacaag
     2221 cgcaaagtcc ggcgcgacct cgccgacgtg ttctgcacga cgagcgaagg cgtgcgcgct
     2281 gaccgcgcca agcaatggaa gctggtggac cagacggcga agccgcaggc gttcaaggat
     2341 gcggtgagag aacgtgccgc ggcgctcagc gcact
//
LOCUS       DASPZD010000369         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_635304, whole genome shotgun sequence.
ACCESSION   DASPZD010000369 DASPZD010000000
VERSION     DASPZD010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635304"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VEM33_07155"
     CDS             <1..557
                     /locus_tag="VEM33_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HYS51640.1"
                     /translation="NGAALKALREALEDGERFVFLWGPGGSGKTHLLRAFSEAAAAAK
                     LEAAAVDDVARLDEAEQIALFDLCNRLRGSGGALAVSGGAPPAQLGLRADLRSRLASG
                     IVLQLRPLSDADKAAALHAHAAARGIALDGELIRYLLTHFDRDMGTQIAALDAIDRYS
                     LQRKRPITLPLLREALGLLDAAKR"
     gene            576..1238
                     /locus_tag="VEM33_07160"
     CDS             576..1238
                     /locus_tag="VEM33_07160"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYS51641.1"
                     /translation="MELALFDLDNTLLSGDSDYEWAQFLVERGVLERAEYEAKNDRFF
                     RQYKEGTLDIRESLEFQLAPLARYPREKLDEWHGEFMRTRIEPMIRAKGMELVRRHLR
                     DNHLCAIVTSTNAFIAAPIARAFGVDHLLATELEVEGGRFTGKPSGTPCFRQGKVSCL
                     AEWLGGRGRTLASFSTSWFYSDSHNDLPLLERVTHPVAVDPDEILRREASARGWQIIS
                     LE"
     gene            1235..>2034
                     /locus_tag="VEM33_07165"
     CDS             1235..>2034
                     /locus_tag="VEM33_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYS51642.1"
                     /translation="MKIVPVLVVALVPASLALAIAFGSVDLGPAQLADALLGRGDEIA
                     REIVWGVRAPRALAGFACGGLLALAGALLQVLLRNPLADPAILGVSGGAASGALAAML
                     LGAGAAGLNLAALAGASIAAGAVFAFSLGRSGWNPYRVLLTGVALASAFGALVGLMLS
                     LAPSSQVQGMLFWLLGDLSPAADPSVALIVLAACAALAFSQSAALDVLILGEDKARSL
                     GVAVARLQAIAFGCATLATVAAVLLGGSIGFVGLVVPHLLRLSGVHRHA"
BASE COUNT          264 a          741 c          701 g          328 t
ORIGIN      
        1 aaaacggcgc ggcgctgaaa gcgctgcgcg aggcgctcga ggacggcgag cgcttcgtct
       61 ttctctgggg cccgggcggg agcggcaaga cccaccttct gcgcgctttt tccgaggcgg
      121 cggcggccgc gaaactcgaa gccgccgcgg tcgacgacgt cgcccgtctc gacgaagcgg
      181 agcagatcgc tctgttcgat ctgtgcaacc ggctgcgcgg ttcgggtgga gcgctcgccg
      241 tgagcggcgg ggcgccgccc gcgcagctcg gcctgcgagc ggatctgcgc tcgcgcctcg
      301 cgtcgggaat tgtgctccag ctgcgcccct tgagcgacgc ggacaaggcc gccgccctcc
      361 acgcgcacgc cgccgcgcgc ggcatcgcgc tcgacggcga gctgatccgc tacctcctca
      421 cgcacttcga ccgcgacatg ggcacgcaga tcgcggcgct cgacgccatc gacaggtatt
      481 cgctgcagcg caagcgcccg atcacgctcc cgctgctcag ggaagcgctc ggcttgctcg
      541 acgccgccaa gcgttgacac gagaaggaca gggacttgga actggcgctc ttcgatctcg
      601 acaacacgct gctctcgggg gacagcgact acgagtgggc gcagttcctg gtcgagcgcg
      661 gcgtgctcga gcgcgccgaa tacgaggcga agaacgaccg tttcttccgc cagtacaagg
      721 agggcacgct cgacatccgc gagtccctcg aattccagct cgccccgctc gcgcgctacc
      781 cccgcgagaa gctggacgaa tggcacggcg aattcatgcg gacccggata gagcccatga
      841 tccgcgcgaa ggggatggag ctcgtgcggc ggcacctccg cgacaaccat ctctgcgcga
      901 tcgtcacctc caccaacgcc ttcatcgccg cgccgatcgc gcgcgcattc ggcgtggacc
      961 acctgctcgc gaccgagctc gaagtcgagg gcggaaggtt cacgggcaag ccctcgggga
     1021 cgccctgctt ccggcaaggg aaggtcagct gtctcgcgga atggctgggc gggcgcggcc
     1081 ggacgctcgc gtcgttttcc acgagctggt tctacagcga ctcgcacaac gacctgccgc
     1141 tgctcgagcg cgtgacacac cccgtcgcgg tggaccccga cgagatcctg cgccgggaag
     1201 ccagcgcgcg cggctggcag atcatcagcc tggaatgaag atcgtcccgg ttctcgtcgt
     1261 cgcgctcgtt cccgcgagcc tcgcgctcgc gatcgcgttc ggcagcgtcg atctcggccc
     1321 ggcgcagctc gccgacgcgc tccttggccg cggcgacgag atcgcgcgcg aaatcgtctg
     1381 gggcgtgcgc gcgcccagag cgctcgccgg gttcgcttgc ggcgggctcc ttgcgctcgc
     1441 gggcgcgctg ctccaggtgc tcctcagaaa tccgcttgcc gatcccgcca ttctgggcgt
     1501 ctccggcggt gcggcgtccg gcgcgcttgc ggcgatgctg ctcggcgccg gcgcggccgg
     1561 cctgaacctc gcggcgctcg ccggcgcctc gatcgcggcc ggcgcggtct tcgcattctc
     1621 cctgggacgc tcgggatgga atccgtaccg ggtgctgctc accggcgtcg cgctcgcctc
     1681 cgcattcggc gcgctcgtcg ggctgatgct ctcgctcgca ccttcctcgc aggtgcaggg
     1741 aatgctcttc tggctgctcg gcgacctctc gcccgcggcc gacccgagcg tcgcgctcat
     1801 cgtgctcgcg gcgtgcgcgg cgctcgcctt ctcgcagtcg gccgcgctcg acgtgctgat
     1861 tctcggcgaa gacaaggcgc gctcgctcgg cgtcgcggtg gcgcggctgc aggccatcgc
     1921 cttcggctgc gcgacgcttg ccaccgtcgc cgcggtcctg ctcggcggct cgatcggttt
     1981 cgtggggctg gtggtgccgc acctgctgcg gctctcgggc gtgcaccgcc atgc
//
LOCUS       DASPZD010000370         5868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_635425, whole genome shotgun sequence.
ACCESSION   DASPZD010000370 DASPZD010000000
VERSION     DASPZD010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635425"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..657
                     /locus_tag="VEM33_07170"
     CDS             79..657
                     /locus_tag="VEM33_07170"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYS51643.1"
                     /translation="MTPTRLCAALVFTLAVAPGLVRPAAAEAIQTRIERQGEYITVSA
                     SALMQVDARIAWEVLSDYDNLAQFIPDLKSSRVVSRDGNRVRVEQKGEIGFFFYWQPV
                     DVTLEVLEQPPHRIDARRIAGNIRELETRYELGTSAAGVRFDYSGRFIPDFSVPPLIG
                     MPIVRRIVERRFRAMVEEIVRRDALERGRPKD"
     gene            complement(695..1822)
                     /locus_tag="VEM33_07175"
     CDS             complement(695..1822)
                     /locus_tag="VEM33_07175"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYS51644.1"
                     /translation="MKKLLIASAVSAAFAAPTVALAQAARAPTLSQVLDASGISVSGY
                     LDAGYSYANRNVETGFSNRVFDSQNNSFALHQFGLTVAKQPTRGFGGVVNITAGTDAQ
                     FIHSFPEGASTNTFDLTQAYGQYASGSLTLMAGKFTTLAGTEVIASPSNMNFSRSMLF
                     GAVPFTHTGVRAGYAVSNTVTLYGGLNNGWDQLTDANRGKTLELGASIVPIRPLTINV
                     SGYFGKETATPPGTPGGATEDQRSLLDVVATWTINNNMSAGGEILYVSQDGVVGGTAK
                     YNGLAGYFTYNLNREWRIAARAELFDDKNNFHFGPNNQLPNTDVKYNELTGTLAYLPN
                     SSVELRGELRFDQANKAVFINSDGSTSKSLQTLGLQGIYKF"
     gene            2053..2922
                     /locus_tag="VEM33_07180"
     CDS             2053..2922
                     /locus_tag="VEM33_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYS51645.1"
                     /translation="MDFFQLPCCGGLMGAARGAAMMEAISTLPVGVFRLKAHNSNVNL
                     HVSTYNIHKGFSQFNRRMMIHELRDHLQLLGADIIFLQEVQGLHSRHAARFEDWPDEP
                     QYEFLADQVWPEFAYGRNAVYDHGHHGNAILSRYPIVRAENQDVSHHALERRGMLHCE
                     IELPEMPQRLHCICVHLALHERGRRHQVGAIIERLHREVPDDAPVVVAGDFNDWRNRA
                     GKRLEEDLGLEEACRDHRGKPVRTFPSAFPMLRLDRIYVRGFRVRHTHVHHGPMWRRL
                     SDHAALSAHLQLE"
     gene            2933..4093
                     /gene="clsB"
                     /locus_tag="VEM33_07185"
     CDS             2933..4093
                     /gene="clsB"
                     /locus_tag="VEM33_07185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HYS51646.1"
                     /translation="MAVEFLPGNRLTLLRSGVEYFPALEAAIREAQREIFLETYIYAG
                     DATGERITRALCGAARRGAAVHVLVDGFGSRDMPEALARELRDAGARLLVFRPEVWRF
                     PFRRDRLRRMHRKIAVADGRVAFVGGINIIDDMDTPRQKPPRYDYAVRIEGPLLGPIQ
                     DSAVRLWNRIALARLQHAWRVSHPVPPHVARAGSQRAALAVRDNLRHRRDIEEAYLAA
                     IGSAQQEIIIASAYFFPGRRFRRALVGAAKRGVRVVLLLQGRVEYVLLYYASRALYGT
                     LLEAGVEIYEYQKSFMHAKVAVIDGHWATVGSSNIDPFSLLLAREANVVVEDRRFAAE
                     LRSRLRADIEHGARVVAKTRWFRQPLWKRVPNWIGYGLARFAIGMFGFGGRF"
     gene            4176..>5868
                     /gene="fusA"
                     /locus_tag="VEM33_07190"
     CDS             4176..>5868
                     /gene="fusA"
                     /locus_tag="VEM33_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYS51647.1"
                     /translation="MPKYSTESIRTVALVGHTGAGKTTLAEALLAKAGAIATAGSVEK
                     GNTVCDFDPLEKEYRHSLNSALVNFAWKNVHIHLIDTPGSPDFMGQAIGALAAVETAV
                     IVVNAETGIQLATDRMLKWAAKRNLCRMILINRIDAENADLPAMLARIQQAVGRECLP
                     INLPAKGGKEVVDCFFSPDGEADFSSVKDAHSALVDQVVEVDDALMEKYLEQGEVSPE
                     QLHAPFEKALREGHLIPVCFASAKNGAGVAALLDVFASLAPNATEGNAPPFLKGEGAE
                     AQEFYAVPGAKAHVLAHVFKVIMDPFVGKVGIFRVHQGTITPNTQLFVGDGRRPFKVG
                     HLFTIQGKDYVETDALVPGDIGAVTKVEEIEFDRVLHDSHDEDHIHLRPLEFPVPMHG
                     IAVETKKKGDEQRLFEVLHKLEMEDPCFRLERHATTNETVIRGLGDLHMRAKLDKMVQ
                     QYKMELVTKPPKIPYRETITRLAEGHCRHKKQTGGAGQFGEVFLRVEPLPRGKGYEFV
                     DKVKGGVIPNVFIPAVEKGVKMALDSGVIAGYPVHDIRVTVYDGKHHAVDSKEVAF"
BASE COUNT          992 a         1917 c         1966 g          993 t
ORIGIN      
        1 aggctgggcg tcagcgcccg gtaagtccgc gaggcttgcc gcgcaacgcg ccgctgcggc
       61 atgctgtcga gcatgtccgt gactccgacg cgcctctgcg ccgctctcgt cttcacgctc
      121 gccgtcgcgc cgggccttgt gcgccccgcc gccgccgagg caatccagac ccggatcgag
      181 cggcaaggag aatacatcac ggtcagcgca tcggcgctga tgcaggtcga tgcgcgaatc
      241 gcctgggagg tgctctcgga ttacgacaat ctcgcccagt tcatcccgga cctaaaaagc
      301 tcgcgcgtcg tgtcgcgcga cggcaaccgc gtccgggtgg agcagaaggg cgaaatcggg
      361 ttttttttct actggcaacc cgtggacgtg acgctcgaag ttctggagca gccgccgcac
      421 cggatcgacg cgcgccgcat cgccggaaac atccgcgagc tggaaacgcg ctacgagctc
      481 gggacttcgg ctgcgggggt gcgcttcgac tacagcgggc gcttcatccc ggacttttcc
      541 gtgcctccgc tgatcggcat gccgatcgtg cgcaggatcg tcgagcgccg cttccgcgcc
      601 atggtcgagg agatcgtgcg gcgcgacgcg ctcgagcgcg gccggccgaa ggactagtgg
      661 tcccccggcc ggtttcgcgc ttgcgctgag cggatcagaa cttgtagatg ccctggagcc
      721 cgagcgtctg caaggacttg gaggtcgagc cgtccgagtt gatgaagacg gctttgttcg
      781 cctggtcaaa gcgaagctcc ccgcgcagct cgacgctgct gttcggcagg tacgccaggg
      841 tgccggtcaa ctcgttgtac ttgacatcgg tattgggcaa ctggttattg gggccgaagt
      901 ggaagttgtt cttgtcgtcg aacagctccg cgcgcgccgc gatacgccac tcccggttga
      961 ggttgtaggt gaagtagccc gcgaggccgt tgtacttcgc cgtcccgccc acgactccgt
     1021 cctggctgac gtacaggatt tccccgccgg cgctcatgtt gttgttgatg gtccaggtcg
     1081 cgacgacgtc gaggaggctg cgctggtcct cggtggcccc gccgggcgtt cccggcggcg
     1141 tcgccgtttc cttgccgaag taaccggaga cgttgatggt cagcggcctg atgggaacga
     1201 tcgacgcccc gagctcgagc gtcttgcccc tgttcgcgtc ggtgagctgg tcccagccgt
     1261 tgttgaggcc gccgtagagg gtgaccgtgt tgctcacggc atagcccgcg cgcacgccgg
     1321 tgtgcgtgaa cggcacggcg ccgaagagca tcgagcgcga gaagttcatg ttgctcgggc
     1381 tcgcgatcac ctccgttccg gcgagggtcg tgaacttgcc cgccatcagg gtcagcgagc
     1441 cgctcgcgta ctgtccgtag gcctgggtga ggtcgaaagt gttcgtggac gctccctcag
     1501 ggaaggagtg gatgaactgg gcgtccgtcc ccgccgtgat attgaccacg ccgccgaagc
     1561 ctcgcgtggg ctgcttggcc acggtcaggc cgaactggtg cagcgcgaag gagttgttct
     1621 gcgaatcgaa cacacggttg gaaaaaccgg tttcgacgtt cctgttggcg taggaatagc
     1681 cggcatcgag gtacccgctc acgctgatgc cggaagcatc gagcacctgc gagagcgtgg
     1741 gagccctggc cgcctgggcc agcgccaccg tgggggccgc gaacgcggcc gagactgccg
     1801 atgcgatgag aagcttcttc atagagcgtc ctttcgtggg tggggaaaat gcttccgctg
     1861 ccttttattt cggcttcaaa cctgccaggc tgcttgatgc cgggtcgcat cggacaatcg
     1921 cccgaaacgc cctcccccta cttgcctgat cccgaccaac gtttacctgg tttcgctcgt
     1981 gccgcactcc tcaagaatgt gaaaaattgc cggagctgca accccggaaa tccgcgcaag
     2041 gatagaccaa aaatggattt tttccaactc ccctgttgcg gcggcctcat gggggcggcg
     2101 cgcggggctg ctatgatgga agcgatctcg acccttccgg tcggcgtgtt ccgcctcaaa
     2161 gcgcacaatt ccaacgtgaa tttacacgtc tccacctaca acatccacaa gggcttttcg
     2221 cagttcaacc gccgcatgat gatccacgag ctgcgcgacc acctgcagct cctcggcgcg
     2281 gacatcatct tcctgcagga agtgcagggg ctgcacagcc gccacgcggc gcgcttcgag
     2341 gactggccgg acgagccgca atacgagttt ctcgccgacc aggtgtggcc ggaattcgcc
     2401 tacgggcgca acgccgtcta cgaccacggc caccacggca acgcgatcct cagccgctac
     2461 ccgatagtgc gagccgaaaa ccaggacgtc tcgcaccatg ccctggagcg ccgcggcatg
     2521 cttcactgcg agatcgagct gccggagatg ccgcagcgcc tgcactgcat ctgcgtgcac
     2581 ctcgcgctgc acgagcgcgg gcgtcgccac caggtcggcg cgatcatcga gcgcctccat
     2641 cgcgaagtgc ccgatgacgc gccggtcgtc gtcgcgggcg acttcaacga ctggcgcaat
     2701 cgggccggca agcgcctcga agaggacctg ggcctggagg aggcctgccg cgaccaccgc
     2761 ggcaagccgg tgcgcacctt cccgagcgcc ttcccgatgc tgcgtctgga ccgcatctac
     2821 gtgcgcggat tccgcgtgcg ccacacgcac gtgcaccacg ggccgatgtg gcggcgcctg
     2881 tccgaccacg ccgccctttc cgcgcacctg cagctcgagt aagtcgccag gcatggcggt
     2941 cgagttcctg cccggaaacc ggctgacgct gctgcgttcg ggcgtggagt atttccccgc
     3001 gctcgaagcc gcgatccgcg aggcgcagcg cgagatcttc ctcgagacct acatttacgc
     3061 cggcgacgcc accggggaac gcatcacccg ggccctgtgc ggggccgcgc ggcgcggggc
     3121 ggcggtgcac gtgctcgtgg atggcttcgg ctcgagggac atgccggaag cgctggcgcg
     3181 cgagctgcgc gacgcgggcg cgcgcctgct cgtgttccgc ccggaggtct ggcgcttccc
     3241 cttccggcgg gaccggttgc ggcgcatgca ccggaagatc gcggtggcgg acggacgcgt
     3301 cgctttcgtc ggcggcatca acatcatcga cgacatggac acgccgcggc agaaaccgcc
     3361 gcgctacgac tacgccgtga ggatcgaggg gccgctgctc gggccgatcc aggactcggc
     3421 tgtgcgcctg tggaaccgca tcgcgctggc gaggctccag cacgcctggc gcgtcagcca
     3481 cccggtcccg ccgcacgtgg cgcgcgcggg cagccagcgc gcggccctcg ccgtgcgcga
     3541 caacctgcgc caccgccgcg acatcgagga ggcctatctc gcggcgatcg ggtcggcgca
     3601 gcaggagatc atcatcgcga gcgcctactt ctttcccggc aggcgctttc gccgcgcgct
     3661 cgtcggcgcc gccaagcggg gcgtgcgcgt agtgctgctg cttcaggggc gcgtcgagta
     3721 cgtgctgctc tattacgcct cgcgagccct ctacggaacg ctgctcgagg caggcgtcga
     3781 gatctacgag taccagaaga gcttcatgca cgccaaggtc gcggtgatcg acggccattg
     3841 ggcgacggtg ggctcctcca acatcgatcc gttcagcctg ctgctcgcgc gcgaggcgaa
     3901 cgtggtggtc gaggaccgcc gctttgcagc cgagcttcgt tcgcggctgc gcgccgacat
     3961 cgagcacggc gcccgcgtgg tggcgaagac ccgctggttc aggcagcccc tgtggaagcg
     4021 cgtcccgaac tggatcggct acgggctggc gcgcttcgcg atcggcatgt tcggctttgg
     4081 cgggcggttt tgacgggccc gctttgacga cctatttgcg gcgcgggtag agtattaggg
     4141 cgcagccgtt cggctcatcc gggaggaaag tccgcatgcc caagtattcg acggaatcca
     4201 ttcgtaccgt agcgctggtg ggccacaccg gcgcgggcaa gaccacgctc gcggaggctc
     4261 tgctcgccaa ggccggagcg atcgccacgg ccggcagcgt cgagaaaggc aacacggtct
     4321 gcgatttcga tccgctggaa aaggagtaca ggcactcgct caactcggcg ctcgtcaact
     4381 tcgcgtggaa gaacgtgcat atccacctga tcgatacgcc cggttcgccc gactttatgg
     4441 ggcaagcgat cggcgcgctc gccgcggtgg agaccgcggt gatcgtggtg aatgccgaga
     4501 ccggaatcca gctcgccacg gaccgcatgc tcaagtgggc ggcgaagcgc aacctgtgcc
     4561 gcatgatctt gatcaaccgc atcgacgcgg aaaacgccga tcttcccgcg atgctcgcca
     4621 ggatccagca ggcggtcggg cgcgaatgcc tgccgatcaa cctccccgcg aaaggcggga
     4681 aggaggtggt ggactgcttc ttcagtcccg acggggaggc ggatttttct tccgtcaagg
     4741 acgcgcactc cgcgctcgtc gaccaggtcg tcgaagtcga cgatgcgctg atggagaagt
     4801 acctcgagca gggcgaggtg agtcccgagc agttgcacgc ccccttcgaa aaagcgctgc
     4861 gcgaagggca cctgatcccg gtgtgcttcg cttcggcgaa gaacggcgcg ggcgtcgccg
     4921 cgctgctcga cgtcttcgcg agcctcgcgc cgaacgcgac cgagggcaac gcgccgccgt
     4981 tcctgaaggg cgaaggcgcg gaggcgcagg agttctacgc cgtgcccggg gccaaggcgc
     5041 acgtgctcgc gcacgtgttc aaggtgatca tggatccctt cgtcggcaag gtggggatct
     5101 tccgcgtgca ccagggaacg atcaccccga acacgcagct cttcgtcggc gacggccggc
     5161 gccccttcaa ggtcgggcac ctgtttacga tacagggcaa ggattacgtc gagaccgacg
     5221 cgctcgtgcc gggggatatc ggcgcggtga ccaaggtcga ggagatcgag ttcgaccgcg
     5281 tgctgcacga ttcgcacgac gaggaccaca tccacctgcg tccgctcgaa tttcccgtgc
     5341 cgatgcacgg catcgcggtc gagaccaaga agaagggcga cgagcagcgc ctgttcgagg
     5401 tgctccacaa gctggagatg gaggatccgt gcttcaggct ggagcgccac gcgaccacca
     5461 acgagacggt gatccggggc ctgggcgacc tgcacatgcg cgccaagctc gacaagatgg
     5521 tgcagcagta caagatggag ctcgtcacca agccgcccaa gatcccgtac cgcgagacca
     5581 tcacgcgcct cgccgagggc cattgccgcc acaagaagca gaccggcggc gcggggcagt
     5641 tcggcgaagt gttcctgcgc gtcgagccgc ttccccgcgg caaaggctac gagttcgtcg
     5701 acaaggtgaa aggcggggtc atcccgaacg tcttcatccc ggcggtggag aagggcgtga
     5761 agatggccct ggattccggc gtcatcgcgg gctatccggt gcacgacatc cgcgtgactg
     5821 tatacgacgg caagcaccac gcggtcgact cgaaagaagt cgcgttcg
//
LOCUS       DASPZD010000371         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1682287, whole genome shotgun sequence.
ACCESSION   DASPZD010000371 DASPZD010000000
VERSION     DASPZD010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682287"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /gene="waaC"
                     /locus_tag="VEM33_07195"
     CDS             complement(<1..817)
                     /gene="waaC"
                     /locus_tag="VEM33_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HYS51648.1"
                     /translation="MKRVLLIKLTSLGDLIHALPALSDAQDARPGLEFDWVIDENFQE
                     IAGWHPAVKTTITTNHREWRGSLAKAETHGSVSKTIEQIRSNEYDLVIDGQGNFKTAL
                     LSLFAKGPRAGFDSHSVREWIAHLAYQRRFAASKNAHAIERLRRLFASALDYAMPASP
                     PDFRIRRERFVEPKVELPADYLVFIHNASWKTKLWPERHWADLIGKCTQAGYRVLLPW
                     GNQQEEARAKRLATGPGVQVLPRLNLSEIGYVIARAKACVCMDTGLSHLAAALD"
     gene            complement(795..1442)
                     /locus_tag="VEM33_07200"
     CDS             complement(795..1442)
                     /locus_tag="VEM33_07200"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HYS51649.1"
                     /translation="MWKVLNPEALRKAPLVAQPFPYLIVDNLIRPEVLPEVVESFPRI
                     PKRGSFPPEAVSYSGRFATLIDEMHSPDLRDLVGDRLGMDLDGRPPMLTIRGRTGKKD
                     GQIHTDSKSKLVTILLYLNSGWSAPEGRLRLLYNDHDLSRYAAEISPDAGRCLIFKVT
                     PNCWHGHEAFEGERRTIQLNYVTSEEARERDLKRHRFSAYLKGLFSRKDEKSSAH"
     gene            1647..>2135
                     /locus_tag="VEM33_07205"
     CDS             1647..>2135
                     /locus_tag="VEM33_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HYS51650.1"
                     /translation="MVNEGKVMEEAKAKALQAALAQIEKQFGKGSIMKMDSAASQDVQ
                     VVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLSVIAQAQKMGGTAAFIDAE
                     NALDPQYAAKLGVKIEELLISQPDTGEQALEIADMLVRSGSVDVIVIDSVAALTPKAE
                     IEG"
BASE COUNT          395 a          624 c          713 g          403 t
ORIGIN      
        1 cgtccagcgc cgccgcgaga tgggagaggc cggtgtccat gcatacgcag gctttggctc
       61 gcgcgatcac gtagccgatt tcggagaggt tgagcctggg caggacctgg actcccggtc
      121 cggtagcgag gcgcttcgct ctcgcctctt cctgctggtt gccccaaggc agcagcacgc
      181 gatatccggc ctgcgtgcac ttcccgatga gatcggccca gtgccgctcg ggccagagct
      241 tggtcttcca gctcgcgttg tggatgaaga cgagatagtc ggcgggaagt tcgactttcg
      301 gctcgacgaa ccgctctctc cggatgcgga aatccggagg agaggcgggc atcgcatagt
      361 cgagcgcgga agcgaacagc cgccgcaggc gctcgatggc gtgcgcgttc ttggacgcag
      421 cgaaccgtct ctggtaggcg agatgagcga tccattcgcg caccgaatgg ctgtcgaagc
      481 cggcgcgcgg tcctttggcg aacagagaga ggagagccgt cttgaaattg ccctggccgt
      541 cgatgaccaa gtcgtattcg ttcgacctga tctgctcgat cgtcttcgaa acggatccgt
      601 gcgtctccgc cttggccaat gagccgcgcc attctcggtg attggtcgtg atggtggttt
      661 tcaccgccgg atgccagccg gcgatttcct ggaaattctc gtcgatgacc cagtcgaact
      721 cgaggcccgg acgtgcgtcc tgcgcgtcgc tcagggcggg gagcgcgtgg atcaggtcgc
      781 ccagcgacgt cagcttaatg agcagaactc ttttcatctt tcctcgaaaa gagccctttc
      841 agataggcgc tgaaccggtg gcgcttgagg tcgcgctcgc gagcttcctc ggaggtgacg
      901 tagttgagct ggatggtgcg ccgctcgccc tcgaaagcct cgtgcccgtg ccagcagttg
      961 ggcgtgacct tgaagatcag gcagcgtccg gcgtcgggcg atatctcggc ggcatagcgg
     1021 gagaggtcgt ggtcgttgta gaggagccgc aggcgcccct cgggcgcgct ccagccggaa
     1081 ttcaggtaga ggagaatcgt gacgagcttc gacttcgaat cggtgtggat ctggccgtcc
     1141 ttctttccgg tgcggccgcg gatggtgagc atcggcggcc gcccatccaa atccatgccg
     1201 agacggtcgc cgaccaggtc gcgcaagtcg gggctgtgca tctcgtcgat cagcgtcgcg
     1261 aaccggcccg aataggaaac cgcctccggc gggaagcttc cgcgcttggg aatgcgcggg
     1321 aagctctcga cgacctccgg aaggacctcg ggccggatca ggttgtctac gatcagatag
     1381 ggaaagggct gcgcgaccaa gggagccttc ctgagcgctt cggggttcag cactttccac
     1441 ataagcgcga ttctattctc cttcggcggt ggcgtctaag tccttgattc ggcatgaggg
     1501 cttgcatttt ctccaaaact gtataaaaat ccaggtcaac cggcttctcg ccgtgcgcgt
     1561 tgggtgcgct gaatggggcg aagatgacgg cggcgggcgc ccgcttcaac gcacaagctg
     1621 caaagcgaat ataggagata atcaaaatgg tcaacgaagg gaaagtcatg gaagaagcca
     1681 aggccaaggc gctgcaggcc gctctcgccc agatcgaaaa acagttcggc aagggttcca
     1741 tcatgaagat ggactctgcg gcctcgcagg acgtgcaggt ggtgtcgacc ggatccctgg
     1801 gcctggacat cgcgctcggc gtgggcggat tgccgcgcgg ccgcgtagtc gagatctacg
     1861 ggcccgagtc ctcgggaaag acgaccctca ccctttcagt catcgcccag gcgcagaaaa
     1921 tgggcggcac cgcggccttt atcgacgcgg aaaacgcgct cgatccgcag tacgcagcga
     1981 agctgggcgt caagatcgag gagctgctga tttcccagcc cgacaccggc gagcaggcat
     2041 tggagattgc cgacatgctg gtgcgctcgg ggtcggtcga cgtgatcgtc atcgattcgg
     2101 tcgccgcgct cacgccgaag gccgagatcg agggc
//
LOCUS       DASPZD010000372         4725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1123117, whole genome shotgun sequence.
ACCESSION   DASPZD010000372 DASPZD010000000
VERSION     DASPZD010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123117"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VEM33_07210"
     CDS             complement(<1..744)
                     /locus_tag="VEM33_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51651.1"
                     /translation="MSAGAELPVLKKSALFGRLALGRAAGVTAITPNKRLSQALMLEF
                     DAFQIGKALSVWEAPDILPFGAFVQRLYEDGLYADLAAELPMLLTPAQEEEVWKQVVG
                     GSGLLAVEGAAAKCRDAWNLANLWRIRPGAGNQDTEAFARWLTHYKKKTENDLDTPRL
                     PDALQRFLPELKRPKLVVAYAFDILPPQTKEFLDALGTEIAFCRPDPRSATVSRAAFD
                     SAKHEIEAAARWARARLEAGATRIGVVIPS"
     gene            complement(770..1093)
                     /gene="fdxA"
                     /locus_tag="VEM33_07215"
     CDS             complement(770..1093)
                     /gene="fdxA"
                     /locus_tag="VEM33_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HYS51652.1"
                     /translation="MAYVVTESCIKCKYTDCVDVCPVDCFREGPNMLVIDPEECIDCT
                     LCVPECPVEAIFAEDDVPPAQRDWIPINAELSKAWKPIVERKPAPADADEWAKVKEKK
                     QLLER"
     gene            1337..1663
                     /gene="trxA"
                     /locus_tag="VEM33_07220"
     CDS             1337..1663
                     /gene="trxA"
                     /locus_tag="VEM33_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HYS51653.1"
                     /translation="MNEFIHHVSDATFEPEVLKSDVPVLVDYWAEWCGPCKAIAPVLD
                     EVAKEYSGKLKVAKVNVDENQEIPKKYGIRGIPTLMLFKNGNVEAQKVGSLSKSQLTA
                     FLDSNI"
     gene            1819..3078
                     /gene="rho"
                     /locus_tag="VEM33_07225"
     CDS             1819..3078
                     /gene="rho"
                     /locus_tag="VEM33_07225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HYS51654.1"
                     /translation="MHLSELKTQHVSQLLEMATSNEIDGANRMRKQELIFALLKNRAK
                     KGESIFGEGTLEVLPDGFGFLRSPDTSYLASTDDIYVSPSQIRRFNLHTGDSIEGEIR
                     TPKDGERYFALVKVDKVNADAPEASKHKILFENLTPLHPDKTLKLERDMKGEENITSR
                     IIDIIAPIGKGQRGLIVSPPKAGKTVLMQQLAHAITSNHPDVVLIVLLIDERPEEVTE
                     MTRTVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTILATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMYEKRIFPAINVNRSGTRREELLIEKEILQKVWVLRKLLYPMDD
                     LEAAEFLVDKIRATKNNGDFFDSMRRG"
     gene            3152..3391
                     /gene="rpmE"
                     /locus_tag="VEM33_07230"
     CDS             3152..3391
                     /gene="rpmE"
                     /locus_tag="VEM33_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HYS51655.1"
                     /translation="MKPKIHPEYKEITVTCSCGNTFTTRSTLGKALHVEVCSSCHPFY
                     TGKQKIVDTAGRVEKFRQKYTKKKAAAKPAKQASA"
     gene            3479..>4725
                     /locus_tag="VEM33_07235"
     CDS             3479..>4725
                     /locus_tag="VEM33_07235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYS51656.1"
                     /translation="MTTLPRVQVLFATLLLAYLLPGLVGHDPWKTEDAIGIGVVHQML
                     EHGRWVVPYLAGEPYLEDGPFHYWVAALTAKLFGLVLSPHDGARLASGVMMAAMLAFV
                     HLAGRELYGRTEGIGAVLALLGCLGLLAHAHETLGDISMLAGQALVWYGIALAPRKPL
                     PAGWLLGAGLVVAALSKGVPALVAPITVALAAPLVSSAWRRREYLMSLIPALLTSLAL
                     CGGWLALAESRSGGLALEWWQANAAQFAVPPAAVLGYWGKTLAWAMWPAWPLALWTLW
                     EGRRRAIVPGTRMLIAAAAVSFAVLVFLKDPGEVYALPLLVPLALLAGAGVPSLRRGA
                     DNALAWFGALSAGLLGALMWLGWFAMMTGYPPTIARNFAKLEPGHVPQFSWTVLAAAL
                     AFTLGWLWLILRGERSVFRGVSF"
BASE COUNT          862 a         1527 c         1485 g          851 t
ORIGIN      
        1 ggaaggaatg acaaccccga tccgcgtcgc gccggcttcc aggcgcgccc tcgcccaccg
       61 cgcggcggcc tcgatctcgt gcttcgcgga atcgaaagcc gctcgcgaaa cggtcgcgga
      121 ccggggatcc ggcctgcaga aagcgatctc cgttccgagc gcgtcgagga actccttcgt
      181 ctgcggcggc aggatgtcga acgcgtaggc gacgacgagc ttcggccgct tcagctccgg
      241 caggaaccgc tgcagcgcat ccggcagccg cggggtatcg aggtcgttct cggttttctt
      301 cttatagtga gtgagccacc gcgcgaaagc ctcggtgtcc tggttcccgg cgccggggcg
      361 gatgcgccac aggttggcga ggttccaggc gtcgcggcac ttagcggccg cgccttcgac
      421 ggcaaggagg ccgctcccgc cgactacctg cttccagact tcctcctcct gcgccggcgt
      481 gagaagcatc ggcagctcgg ccgcgaggtc cgcatagagg ccgtcctcgt agaggcgctg
      541 cacgaatgca ccgaacggca ggatatccgg cgcttcccag accgaaagtg ctttcccgat
      601 ctggaaagca tcgaactcga gcatcagcgc ctgcgacagg cgcttgttgg gggtgatggc
      661 ggtgacgccg gcggcgcggc ccagcgcaag gcgcccgaac agggcgctct tcttcagtac
      721 cggcagctcg gcgcccgcgc tcatgggcgc ctactttagc gcgctttgct cagcgctcca
      781 gaagttgctt cttttccttc accttggccc attcatcggc gtccgccggc gcgggcttgc
      841 gctcgacgat gggcttccag gccttggaga gctcggcatt gatgggaatc cagtcgcgct
      901 gcgcgggcgg gacgtcatcc tcggcaaaga tggcctcgac ggggcactcc gggacgcaca
      961 aggtgcagtc gatgcactcc tccgggtcga tcaccagcat gttggggccc tcgcgaaagc
     1021 agtcgaccgg gcacacgtcg acgcagtcgg tgtacttgca cttgatgcag ctctcggtga
     1081 cgacgtatgc catggtgagg acgagcccgc ccggcccgaa cgattcccgc gcatttcgcg
     1141 aagacagcgg gtatgttatc acgcacggtc ccgggttccg ccgttttgtc ctgaaaaaat
     1201 agccccttac gccaaacggg tttgtgcttt tcctcgccgt gaaattccgc tagcatagcc
     1261 ttgaacgtac gggttcgcgc ccatacatat cgaacgtgca gcgtccgctg caaattcccg
     1321 tttcgcagag gaagcaatga acgaattcat ccaccacgta agcgatgcga ctttcgagcc
     1381 cgaagtcctg aagtccgacg tccccgtgct ggtggactac tgggcggaat ggtgcggtcc
     1441 gtgcaaggcg atcgccccgg tgctcgacga ggtcgccaag gagtactcgg gcaagctcaa
     1501 ggtcgccaag gtcaacgtgg acgagaacca ggaaatcccg aaaaagtacg gtatccgagg
     1561 gataccgacg ctcatgcttt tcaagaacgg caacgtggag gcgcagaagg tggggtccct
     1621 gtccaaatcg cagctcaccg catttcttga cagcaacatc taaagactct attcttcacg
     1681 caaaaggcgg gcggcaagct tagccgccct ccaaccggcg cctgcaaagg gctgtatcca
     1741 ggcgccaatc ctccccgttt cggttccctg tttttccgtt cgaacggtct gaggtcgttc
     1801 atccggcttg ggctctttat gcacttatcc gaactcaaga cacagcacgt aagccagctg
     1861 ctcgaaatgg cgacgtcgaa cgagatcgac ggcgccaacc gcatgcgcaa gcaggagctg
     1921 atctttgcgc tcctgaaaaa ccgcgccaag aagggtgagt ccatattcgg cgaaggcacg
     1981 ctggaggtgc tgcccgacgg cttcggcttc ctgcgctcgc ccgatacgtc ctacctggct
     2041 tccaccgacg acatctacgt cagcccctcg cagatccggc gattcaacct gcacacgggc
     2101 gattcgatcg agggcgagat ccgcacgccc aaagacggcg agcgctactt cgcgctggtg
     2161 aaagtcgaca aggtcaacgc cgacgcgccg gaggcctcga agcacaagat cctcttcgag
     2221 aacctcaccc cgctgcaccc ggacaagacc ctgaagctcg aacgcgacat gaagggcgag
     2281 gaaaacatca ccagccgcat catcgacatc atcgccccga tcggcaaggg gcagcgcggc
     2341 ctgatcgtct ccccgcccaa ggcgggcaag acagtgctga tgcagcagct cgcccacgcg
     2401 atcacgtcga accacccgga cgttgtgctg atcgtcctct tgatcgacga gcggcccgag
     2461 gaagtgaccg agatgacgcg cacggtcaag ggcgaggtcg tcgcctcgac gttcgacgag
     2521 cccgcctcgc gccatgtgca ggtcgccgaa atggtgatcg aaaaagcgaa gcgcctggtc
     2581 gagcacaaga aggatgtcgt gatcctgctc gactcgatca cgcggcttgc acgcgcttac
     2641 aacaccgtgg tgcccgcctc cggcaaagtg ctcacgggcg gcgtcgatgc gaatgcgctg
     2701 cagcgcccga agcgcttctt tggtgcggcg agaaacatcg aggaaggcgg ctcgctcacc
     2761 atcctcgcca ccgccctgat cgacacgggc tcgcgcatgg acgacgtgat ctacgaagag
     2821 ttcaagggca cgggcaacat ggaaattcac ctcgaccggc gcatgtacga gaagcgcatc
     2881 tttccggcga tcaacgtcaa ccgctcaggc acccgccgcg aagagctcct gatcgagaag
     2941 gagatcctgc agaaggtgtg ggtgctgagg aagctcctct accccatgga cgacctcgag
     3001 gctgcggagt ttctggtcga caagatccgc gcgaccaaga acaacggaga cttcttcgat
     3061 tcgatgcgca ggggttgaaa aacttgtccg ggcgcctgat ttacaggata atttcgcccc
     3121 actcaacgct ctgcgcagca aggacaacac catgaaaccc aaaatccatc ccgaatacaa
     3181 ggaaatcacc gtaacctgca gttgcgggaa cacgttcacg acccgctcca cgctgggcaa
     3241 ggccctgcac gtggaagtgt gctcgtcctg tcacccgttc tacacgggca agcaaaagat
     3301 cgtcgacacg gccggccggg tggagaaatt ccgccagaaa tacaccaaga agaaggcggc
     3361 ggcgaagccc gccaagcaag cctccgcgta ggcgtgtcgc gccgcgcgct ctgcgaaagg
     3421 cagcttcggc tgcctttttt ctttcgggtg atcggtcgag cgggctaagg taaacgcaat
     3481 gactacgctt ccccgggtgc aggttctgtt cgcgacgctc ctgctcgcgt acctgctgcc
     3541 cggcttggtc gggcacgatc cctggaaaac cgaagacgcc atcggcattg gtgtcgtgca
     3601 ccagatgctc gagcacgggc gatgggtcgt gccgtatctg gctggcgaac cgtacctgga
     3661 ggatggaccg ttccattact gggtcgcggc gctgaccgcg aagctgttcg gactggtcct
     3721 ctccccccac gacggggcac gcctggcaag cggtgtgatg atggcggcga tgctcgcctt
     3781 cgttcacctc gcgggccgag agctctatgg cagaaccgag gggataggcg cggtgctcgc
     3841 gctgctcggc tgtctcggcc tgctcgctca cgcccatgaa acgctcggcg acatcagcat
     3901 gctcgcggga caggccctcg tctggtacgg catcgcgctc gcgccgcgca aaccgctccc
     3961 tgcgggttgg ctcctcggcg cggggctcgt cgtcgcagcg ctgagcaaag gagtccccgc
     4021 gctagtggct ccaatcaccg tcgcgctcgc cgcgcctctc gtgtcttcgg cgtggcgccg
     4081 gcgcgagtac ctgatgagct tgattccggc cctgctgacg agtctcgcgc tgtgcggcgg
     4141 ctggctcgcg ctcgcggaaa gccgctccgg cgggctcgcc ctcgagtggt ggcaggccaa
     4201 cgcagcacaa ttcgccgtcc ctcctgcagc agttctcggc tactggggaa agacgctcgc
     4261 ctgggcgatg tggcctgcgt ggccgctcgc gctatggacc ctgtgggagg gacgccgccg
     4321 cgcaatcgtg ccgggaacgc gcatgctgat cgcggccgcc gccgtcagct ttgccgtgct
     4381 cgtgtttctg aaggatccgg gcgaggtcta cgctctgccg ctgctcgtgc cgcttgcgct
     4441 tcttgccggt gccggggtgc cgagcttgcg ccgcggcgcc gacaacgcgc tcgcgtggtt
     4501 cggcgccctg agcgccgggc tgctcggcgc gctcatgtgg ttgggctggt ttgcgatgat
     4561 gacgggatac ccgccgacca tcgcgcgtaa tttcgccaag ctcgagcccg gccatgtgcc
     4621 gcagttttcc tggacggtgc tcgccgccgc gttggcgttt acgctgggct ggctctggtt
     4681 gatcctgagg ggcgagcgct cggtattccg cggtgtgagc ttctg
//
LOCUS       DASPZD010000373         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1481077, whole genome shotgun sequence.
ACCESSION   DASPZD010000373 DASPZD010000000
VERSION     DASPZD010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481077"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1321)
                     /locus_tag="VEM33_07240"
     CDS             complement(47..1321)
                     /locus_tag="VEM33_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYS51657.1"
                     /translation="MQKPTSQRRLWTPSLTAFGGRRSRRKFYYEAFIPPPIADLELPL
                     QNDVASVVTEAEVAVRGLNESSPRLGSLEVLARQLLRAEAVASSRIEGLEMSHRRLAR
                     AAAAPEHADARARAVLGNVRAMERAIEIGSTARTITVARLLELHRELFRDTPDAARAG
                     KLRETQNWLGGRENSPLGAEFIPPPEDRIDGLLRDLCEFVNRDDIPAVAQAGVAHAQF
                     ETIHPFFDGNGRIGRSLIHVVFRRRGLAPRYVPPVSLILATDQKAYVNGLTVFRSFSN
                     KGLNDWVATFAQATRTAGVQAARFAEDLAELQERWRARAGVKRRGSAVDQLITKLPAE
                     PVLDITRAAALTGSVYEAARLAVERLVGARVLYPVGSRRRDRLFEARDLFDLVDDLER
                     RLATPPGSKRTVRRAPHRRPPASALARPRAEA"
     gene            1422..>2061
                     /locus_tag="VEM33_07245"
     CDS             1422..>2061
                     /locus_tag="VEM33_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HYS51658.1"
                     /translation="MKIWLLAVALLLSACTTPARPSATPSVTPSPTAAATASPAPTPT
                     PTAECAVSVLATMTEEQRVGQLFLLGLANDQLGAAEQNAIRTYHFGSVWFVEQSAAGA
                     PAIRAVADAVQALATQDTTAGVRFFVAANQEGGIIQSLKGPGFSTIPSALDQSAIDPA
                     TLQTSAALWGRELASAGVNLNFAPVLDVVPPGTDAQNEPIGALRRGYGHDPAT"
BASE COUNT          370 a          674 c          673 g          344 t
ORIGIN      
        1 gtcagagcgg tggaatgtgc agcctgcacg cccaaaccga catttgtcat gcttcagcac
       61 gcgggcgcgc cagagccgac gcagggggcc gcctgtgtgg ggcacgccgt acggtccgct
      121 tgctgcctgg aggggtcgcg agacgtcgct cgaggtcatc gacgaggtcg aataggtccc
      181 gggcttcgaa taggcggtcg cgcctgcgcg accccaccgg atagaggaca cgggccccga
      241 caagccgctc gacagcgagc cgggcagcct catacacgga cccggtcagc gccgcggctc
      301 gcgttatgtc caacacgggc tctgccggta gcttcgtgat cagctggtcg acggccgaac
      361 cccgccgctt gacgcccgca cgagctcgcc agcgttcctg gagctcggcg aggtcctctg
      421 caaaccttgc tgcctggact ccggccgtcc gggtcgcctg cgcaaatgtc gcgacccagt
      481 cgttgagccc cttgttggag aacgatcgaa acaccgtgag tccgttgaca taggccttct
      541 ggtctgtagc caagatcaga cttactggag gaacgtatct cggcgcaaga cctctacgtc
      601 ggaagacaac gtgaatcaaa gaacgcccga tccggccatt gccgtcaaag aaggggtgga
      661 tagtctcgaa ttgggcgtga gccacgcctg cttgcgcgac cgccggtatg tcgtcgcgat
      721 tcacaaactc gcacaggtca cgaaggaggc catcgatccg gtcctctggc ggaggtatga
      781 actcggcacc gagaggggaa ttctcacggc cccctaacca gttctgtgtt tcgcggagtt
      841 ttccggcgcg cgccgcgtcc ggggtatccc ggaatagctc tcggtgcagt tcgaggaggc
      901 gtgcgacagt gattgtccgc gcggtcgagc caatctcgat ggcgcgttcc atggcccgga
      961 cgttgccaag gaccgcgcgc gcgcgggcgt ccgcatgttc gggggctgcg gcagcgcgcg
     1021 ctaaccgcct gtgagacatc tcgagccctt caatccgcga tgaagcgaca gcctcggccc
     1081 gaagtaactg gcgggcgaga acctcaagag acccgagccg tggcgagctc tcgttcaagc
     1141 cgcgcacggc aacttccgcc tcggttacaa ccgaggcgac atcgttttgc agcggtagct
     1201 cgagatcggc gattggcggg ggaatgaacg cctcgtagta aaacttcctt cggctgcgcc
     1261 ggccgccgaa agcggtaagg cttggtgtcc aaaggcgccg ttgactcgtg ggtttttgca
     1321 tcgccggtca tttcctaact ttgtctagat gacgcgttac acaatgttag cacaataact
     1381 ttgtgtagcg aacgacgtac acaaagatca agatcgaggc gatgaaaatc tggctcctcg
     1441 ctgtcgcgct cctcctgtcg gcgtgcacga cgccggcgag gccgagtgcc accccgagcg
     1501 tgacaccaag tccgaccgct gccgcgacgg caagccctgc gccgactccg actcccaccg
     1561 ccgagtgcgc tgtgagcgtg ctcgcgacca tgaccgagga gcagcgcgtc gggcagctct
     1621 tcctgctcgg gctcgcgaac gatcagctcg gcgcggccga gcagaacgcg atccgcacgt
     1681 atcacttcgg gtcggtctgg ttcgtcgagc agagcgcggc tggcgcgccc gcgatccgcg
     1741 cggtcgccga cgcggtgcag gcgctggcga cgcaggacac gaccgctggc gtccgcttct
     1801 tcgtggcggc gaatcaggaa ggcggcatca tccagtcgct caaaggtccg ggcttcagca
     1861 cgatcccgag cgccctggac cagagcgcga tcgaccccgc gacgttgcaa acaagcgcgg
     1921 cgctctgggg acgcgagctc gcgagcgcgg gcgtcaacct caacttcgcg ccggtcctcg
     1981 acgtggtccc gcccgggacc gacgcgcaga acgagccgat cggcgcgctc cgtcgcggct
     2041 acgggcacga ccccgcgacg g
//
LOCUS       DASPZD010000374         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1029565, whole genome shotgun sequence.
ACCESSION   DASPZD010000374 DASPZD010000000
VERSION     DASPZD010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029565"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="mreC"
                     /locus_tag="VEM33_07250"
     CDS             complement(<1..671)
                     /gene="mreC"
                     /locus_tag="VEM33_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYS51659.1"
                     /translation="MDYQPPPFFSRGPAPLVRLGFFVCLAVLLMVLDARFRYAESLRQ
                     VIALLTYPLQRVALAPGELFGAAAGFFTTQVSLKQENEQLKAKQLQAANELLTVQALR
                     SENAQLRRLLEARERLPRESTLAEILYQGRDPFSRKVIIDKGRQQGIQPGQAVIDDLG
                     VVGQVTRVYPLLSEVTLITDKEQRTPVQVVRNGLRAIIYGGGGRGTLDLSYTAANADV
                     LADDVL"
     gene            complement(771..1814)
                     /locus_tag="VEM33_07255"
     CDS             complement(771..1814)
                     /locus_tag="VEM33_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYS51660.1"
                     /translation="MFGFLGGYFSDDMAIDLGTANTLIYVRGKGIVLDEPSVVAIRQE
                     GGPHSKKTIQAVGKEAKMMLGKTPGNIVAIRPMKDGVIADFTVTEQMLKQFIKKVHQS
                     RLFSPSPRIIICVPCGSTQVERRAIREAAMEAGASQVFLIEEPMAAAIGADLPVAEAT
                     GSMVVDVGGGTTEVGVISLGGMVYSGSVRVGGDKFDEAIVNYIRRNYGMLIGDTTAEN
                     VKKEIGSAFPGSEVREMEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVGAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLMEETGLPVIVADDPLTCVVRGSGKAL
                     ERMDKLGSIFASE"
     gene            1894..2181
                     /gene="gatC"
                     /locus_tag="VEM33_07260"
     CDS             1894..2181
                     /gene="gatC"
                     /locus_tag="VEM33_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYS51661.1"
                     /translation="MPLSSKEVRRIAELARIGVSDAEAAAVQAQLNDIFELIGRMQKV
                     DTEGVAPMSHGQDLKLRLRDDAVTEGDQRELFQSVAPQVEAGLYLVPKVIE"
BASE COUNT          418 a          748 c          724 g          439 t
ORIGIN      
        1 agcacgtcat cggctagaac gtcggcgttc gcggccgtat agctcaagtc gagcgtacct
       61 ctgccgccgc cgccgtagat gatggcgcgc aggccgttcc tcacgacctg tacgggagtt
      121 ctctgctcct tgtcggtgat cagggtcacc tccgagagca gcggatatac gcgcgtcacc
      181 tgcccgacta cgccgaggtc gtcgatgacc gcctgtcccg gctggatgcc ctgctggcgg
      241 cccttgtcga tgatcacctt gcgcgagaag ggatcgcgcc cctgatagag gatctcggcg
      301 agggtggact cgcgcggcag gcgctcgcgt gcctcgagca agcgcctcag ctgggcgttt
      361 tcggagcgca gcgcctggac ggtcagcagc tcgttagccg cctgcagctg cttcgccttg
      421 agctgctcgt tctcctgctt gagcgacacc tgtgtcgtga agaatccggc ggcggcgccg
      481 aacagctccc ccggagcgag cgcgacgcgc tgcagcggat acgtgagcag ggcgatgacc
      541 tgacgcaggg actcggcgta tcgaaaacgc gcgtccagca ccatcaggag gaccgcgaga
      601 cacacaaaaa accccaggcg gacgagcggc gcgggaccgc ggctgaagaa cggcggcggc
      661 tggtaatcca tgggtctaag ggcttgaagg gcctgggcga tgcgaacccg ttacgaacat
      721 cgcgtgcaac actcgctttt ccccagtctc gggcctcgag tttccggccg ctattcgctt
      781 gcgaatatgc tccccagctt gtccattctt tccagcgcct tgcccgagcc gcgcacgacg
      841 caggtgagcg gatcatcggc gacgatcacg ggcagccccg tctcttccat gaggaggcga
      901 tcgaggtcgc gcaagagcgc accgccgccg gtgagcacca tgcctttttc ggcgatgtcg
      961 gcgcccagct cgggcggggt ctgctccagc gccgacttca ccgcacccac gatctggttg
     1021 agcggctcgg tgagagcctc caggatctcg ttcgaggaaa tggtgaagct cctcgggatg
     1081 ccctccgcga ggttgcggcc cttcacttcc atctcgcgca cttcggatcc ggggaaggcc
     1141 gagccgatct ccttcttgac gttctccgcg gtggtgtctc cgatcagcat tccgtagttg
     1201 cggcggatgt agttgacgat cgcctcgtcg aacttgtcgc ccccgacgcg caccgagccc
     1261 gagtagacca tgcctccgag cgagatcacg ccgacttcgg tcgtgcctcc gcctacatcg
     1321 acgaccatcg agcccgtggc ctcggccacc ggcaggtccg cgccgatcgc ggcagccatc
     1381 ggttcctcga tcaggaacac ctggcttgcg ccggcctcca tggcagcctc gcggatcgcg
     1441 cgtcgctcga cctgcgtcga gccgcagggc acgcagatga tgatgcgcgg tgaaggcgag
     1501 aacaggcgcg actggtgcac tttcttgatg aactgcttca acatctgctc ggtgacggtg
     1561 aaatcggcga tcacgccgtc cttcatcggc cggatcgcga cgatgttgcc cggcgtcttg
     1621 ccgagcatca ttttcgcttc cttgccgacc gcctgaatgg ttttcttgga gtgggggccg
     1681 ccttcctggc gaatcgccac gaccgaaggc tcgtcgagca cgatgccttt gccccgcacg
     1741 taaatcagcg tattggccgt acccaggtca atcgccatgt cgtctgaaaa atatccaccc
     1801 aaaaagccga acatcctgct gatttcctta ggaaaggggg ttctcgcttg cgctataata
     1861 cagcaaataa ttttccgctt taagcgattt ttcatgccgc tgtcctcaaa agaggtgcgc
     1921 cgcatcgcgg aactggcccg gatcggcgtg agcgacgccg aggctgcggc ggttcaagcc
     1981 caactcaacg acattttcga gctgatcggg aggatgcaga aggtcgacac cgagggcgtc
     2041 gcgccgatgt cccacggcca ggacctcaaa ttgcgcctgc gcgatgacgc cgtgaccgaa
     2101 ggcgaccagc gggaactttt ccagtcggtc gcgccgcagg tcgaagccgg tctgtatctc
     2161 gttccgaaag tcattgagta aaacctggat gggtgaaggc gtgaaggggg acgggtgctg
     2221 aacgcctcgc tcaaggaact tgccgccgcg ctcgcgcaga aggaaatctc gagcgcagag
     2281 ctgaccaagc tcttcctcga tcgcacctcg agactgaacg gtgcgctca
//
LOCUS       DASPZD010000375         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1321289, whole genome shotgun sequence.
ACCESSION   DASPZD010000375 DASPZD010000000
VERSION     DASPZD010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321289"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VEM33_07265"
     CDS             <1..239
                     /locus_tag="VEM33_07265"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525705.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HYS51662.1"
                     /translation="AEPDQVLKTALDAAQQLAAGPTFAHGMTKKMLREEWAVGLDEAI
                     GMEAKAQAICMQTRDFRRAYEAFVAKEKPVFEGD"
     gene            245..1387
                     /locus_tag="VEM33_07270"
     CDS             245..1387
                     /locus_tag="VEM33_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYS51663.1"
                     /translation="MDRFAHLDWPFFEPRHAELARMAEHWAAASLSYAHDADEDAVCR
                     RLVKDLGAAGFLAHCAPEDSRFDVRSLAVLREVFAFHAGLADFALTMQGLGSGPVVLA
                     GTEQQKRRYVGRAARGEAIAAFALSEPDAGSDVAALATRARRDGAHYVLNGVKTWISN
                     GGIADFYIVFARTGDEAGTKGISAFVVDADAPGLSIEERIEIVAPHPMATLRFSACKV
                     PAANLLGAEGEGFKLAMRTLDIFRTGVAAAALGFARRALAEALLRARERKMFGQALAD
                     FQLTQTKLADMATGIDAAALLAYRAAWMRDVKQKRVTREAAMAKMQATETAQEVVDAA
                     VQICGGLGVMRGHPVERLYREVRALRIYEGATEVQKLIVARELLKE"
     gene            1410..>2205
                     /locus_tag="VEM33_07275"
     CDS             1410..>2205
                     /locus_tag="VEM33_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HYS51664.1"
                     /translation="MGSTAHTDTFARDNLPPRAQWPELIFDLPELRYPGRLNCATELL
                     DRAPERGWARRVALRFAEGELSYAQLLEQSNRIARVLAEDMGVATGNRVLLRGANSPW
                     MAACWFAVMKAGGIAVATMPLLRAKELTEIILKARVTHALCDRRLDAELKAALPACPI
                     LKTAAYWGDDAPDSLETLAARKPGSFTNVDTAAEDVALIAFTSGTTGKPKGIVHSTAG
                     YILQTSMTMKWVFDLREEDTYWCTADIGWVTGHSYIVYGPLAAGATS"
BASE COUNT          354 a          730 c          789 g          332 t
ORIGIN      
        1 tcgccgagcc cgaccaggtg ctgaaaaccg cgctcgacgc cgcgcagcag ctcgccgccg
       61 gcccgacgtt cgcccacggc atgaccaaga aaatgctgcg cgaggaatgg gcggtgggtc
      121 tcgatgaggc gatcgggatg gaggccaaag cgcaggcgat ctgcatgcag acgcgagact
      181 tccgccgggc gtacgaggcc ttcgtcgcca aagagaagcc ggtcttcgaa ggcgattgag
      241 gctcatggac cggttcgcgc atctggattg gccgttcttc gagcctcggc acgcggagct
      301 cgcgcgcatg gcagagcact gggccgcggc gagtctttcc tacgcgcacg acgcggacga
      361 ggatgccgtc tgccgccgac tggtgaagga cctgggcgcg gcggggttcc tcgcgcactg
      421 cgcgcccgag gattcgcgct tcgacgtgcg ctcgctggcg gtgctgcgcg aggtgttcgc
      481 cttccacgcc ggcctcgccg atttcgccct caccatgcag gggctgggca gcggtcccgt
      541 ggtgctcgcc ggcaccgagc agcagaagcg ccgctacgtc gggagggccg cgagaggcga
      601 ggcgatcgca gctttcgcgc tgtccgagcc cgacgcgggc tcggacgtcg cggcgctcgc
      661 cacccgggcc cggcgcgacg gcgcgcacta tgtgctcaac ggggtgaaga cctggatctc
      721 caacggcggc atcgccgatt tctacattgt ctttgcccgc accggggacg aggccgggac
      781 gaagggcatc tcggccttcg tcgtcgacgc cgacgcgccg gggctctcca tcgaggagcg
      841 catcgagatc gtcgcaccgc atccgatggc gacgctgcgt ttttccgcgt gcaaggtccc
      901 ggcggcgaac ctgctgggag ccgagggcga gggcttcaag ctcgccatgc ggaccctgga
      961 catcttccgc acgggcgtcg cggccgccgc gcttggcttc gcgcggcgcg ctctcgccga
     1021 ggcgctgctg cgggcgcgcg agagaaaaat gttcggtcag gcgctcgccg acttccagct
     1081 gacgcagacg aagctcgccg acatggcgac cggcatcgat gccgccgcgc tgctcgcgta
     1141 ccgcgctgcc tggatgcgcg acgtgaagca gaaacgcgtg acccgcgaag ccgccatggc
     1201 gaagatgcag gcgaccgaga ccgcccagga agtcgtcgac gccgcggtgc agatctgcgg
     1261 agggctgggc gtcatgcgcg gtcatccggt ggagaggctg tatcgcgagg tgcgcgcgct
     1321 gcgcatctac gaaggcgcga ccgaggtgca gaagttgatc gtcgcccgcg agctgctgaa
     1381 ggaatagcgt gggggcggcg gaccggacca tgggcagcac cgcgcacacc gacaccttcg
     1441 cgcgcgacaa tctgccgccg cgcgcgcaat ggccggagct gatcttcgat ctgccggagc
     1501 tgcgctatcc cggccgtctg aattgcgcga ccgagctgct cgatcgggcg ccggagcgcg
     1561 gctgggcgcg gcgcgtcgcg ctgcgctttg cggagggtga gctcagctat gcacagctgc
     1621 tcgagcagtc caaccgcatc gcccgcgtcc tcgccgagga catgggcgtc gcaacgggca
     1681 atcgcgttct gcttcgcggc gcgaacagcc cgtggatggc cgcttgctgg tttgcggtga
     1741 tgaaggcggg tggaatcgcg gtggcgacca tgccgcttct gcgggcgaag gagctgaccg
     1801 agatcatcct caaggcgagg gtcacgcatg cgctgtgcga caggcgcctg gacgccgagc
     1861 tgaaggcggc cctgcctgcg tgcccgattc tgaaaaccgc ggcgtactgg ggcgacgacg
     1921 cgcccgactc gctcgaaacg ctcgccgcgc ggaagccggg cagcttcacg aacgtggaca
     1981 cggctgcgga ggacgtggct ttgatcgcat tcacctccgg caccacgggc aagcccaaag
     2041 gcatcgtgca ctcgaccgcc ggctatatcc tgcagacctc gatgaccatg aaatgggtct
     2101 tcgacctgcg cgaagaggac acctactggt gcaccgccga tatcggatgg gtcaccggcc
     2161 acagctacat cgtgtacgga ccgctggccg cgggggccac ttcgc
//
LOCUS       DASPZD010000376         5771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1118159, whole genome shotgun sequence.
ACCESSION   DASPZD010000376 DASPZD010000000
VERSION     DASPZD010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118159"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1073)
                     /locus_tag="VEM33_07280"
     CDS             complement(129..1073)
                     /locus_tag="VEM33_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HYS51665.1"
                     /translation="MKLRRIGLLALALACLSQARAAPFAYVPNERSGTVSVIDTASDQ
                     VVATIETGGKPRGIAATPDGRLLYVSDQPGGSLVVVDLAAREVSDRIAVGKSPEGVGI
                     SRDGAWIAVAVEENNSVSLIRTAAPREATSVRVGGQNPEHVVFSPEGKWLYVSAEDGA
                     AVDVIDVAKRSAVKSIKVGERPRGIGFLPDGSRAYVAAENSDRVYVIDCARAEVIAVI
                     AAGKRANGIAVRPDGKVVFVSNGGDASVSAIDVASGKITATVPVGQRPWNMALTPDGK
                     KLYVANGRSNSVSVIDAGAMTRLRDIPVGEAPWGVVVR"
     gene            1225..3960
                     /locus_tag="VEM33_07285"
     CDS             1225..3960
                     /locus_tag="VEM33_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51666.1"
                     /translation="MDPSLRWRTLDTAHFSVHFADHYRSQAQFVAEVAESVYPRVTGW
                     LNWKPESRTQIVVLDSLDFSNGYASPYPFNFIGIILSPPDEGELLQNREWLELVLTHE
                     FTHIVHLDQARGPALALRGIFGRYLVLPLPFTLFFPSGFPNLLEPNWMIEGLAVYSES
                     DRSKGYGRLGQSQFEGMMRAESARGLRSLREVNAEGRGFPLNRDYLYGSYFFLFLAER
                     YGPDAIPKYVEIYSGRWFPFRVHSTPRFVTGKAMDELWVEYHDWLNARFAVKPREPGK
                     EPDGGGEFLVRDWSITSPLRTSRGDRWYVQGNGYVLPKLMRQTLGGEVEPVRDIEPGT
                     RLAAFPGGELLLSQPEICSNYNYYYDLYRLGPDGALSRVTECGRFRFAAPLDDGRIVA
                     IRVVNGEAEVVLLNSRGGPERSLYRAAPGESLTGLAAKGDTVVVTSLRNERWSLVRIA
                     EGKSSVLVSDRAVKHSPRFGDSADEIYFVADYGNVNNVWSWRRGDRSLARWTEARNGV
                     GEISAPVGGELLVTTIEADGDVLRARSLPDAPLEVRRAAAEAGPSAAPPEGAPPPGGD
                     RAYSAWSSLRPRYWLPIGYAADGAVALGIATDGQDALGLHRYTFAPLYEITQHQALGS
                     ATYVYDNRHGLLLNRFMTVKTLDSGRNIEAYAINETAQWVSLWRQLSLGTRIYWGLGA
                     ALDREILHDLAAGTSTPRDERVLGLVAGVDTRREQFLSEGPSQGQQLRLFAETSNRLR
                     GAYAGNFYRADWRAYLPVYKSVVSLRWNETYSQPEAEPVQLGGSFSEETFALPVLNQR
                     EFPLRGYLSGEAVLTGHRSRLGTIEWRTPISDVDRHLMVPPLGLNRISVSVFFDAGAA
                     WESGAPQRYFKSAGVELLSEVRLGYQLGAQLRAGVAKGFESPGRTVAYLHVGRSF"
     gene            complement(4020..>5771)
                     /locus_tag="VEM33_07290"
     CDS             complement(4020..>5771)
                     /locus_tag="VEM33_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51667.1"
                     /translation="GDGQMGEGRQGSRNQTGVTRVRAAVLARLLGGCCAVIACSLGPA
                     AAQEPATEPKAPLPAEPTPPSTQKIPSFAELEAAGAVIGEVRIATRDIFDLADEKENG
                     ILYRAANALHIQTRAGVVRRQLLFKPGERVSVRLIEETERLMRSSRIFYDVSIVPVAY
                     RDGVVDIEVKTRDTWTLEPGWSASRAGGVNKTGFTLRDTNALGTGVLIAATRSTDADR
                     TETKYTVSQPHAFDGWTAIDYSHSQLSDGQSNAISIVRPFYALDTRWAAGFSASMDTR
                     IDSFFNDAVKQFQDRHRRDTAETFGGWSEGLVEGWTHRYSAGLSYLKETYKGDPDLLP
                     PPNPFPQDQTLVAPFFRYELVQDNYEKVKNRDLIERPEYFAMGTQASVQLGRSLTGLG
                     STQNVWLYSASANDGFRLPSNWVLLGSTSVSGQTGYGPLDRYLASGSMRFYGHPDNRT
                     LTFVSLAADILRDPNSSQLVLGGDTGLRGYPRNYQTGDRRVVFNVEERVYTDWYPFRL
                     FRVGGAVYYDLGRAWSGPGESPSSARWLNDVGFGLRILSARSSFGNVLHVDLAFPLNR
                     DPNIKSFQFLVQTKLSL"
BASE COUNT          935 a         1892 c         1939 g         1005 t
ORIGIN      
        1 cttctcgagc gcggcgccga tgcgagcgcg cgcgacaacc gcggcaagac cgcgctcgcc
       61 atggcgatcg agggcggaca cggggaaacc gaacaactgc tccgcaaggc cggcgccgcg
      121 caatagctct agcggactac cacgccccag ggcgcttcac ccaccgggat gtcgcgcaac
      181 cgggtcatcg ccccggcgtc gatcaccgac acggagttgg agcggccgtt tgcgacgtag
      241 agttttttcc cgtccggggt caacgccatg ttccagggac gctggccaac gggaacggtc
      301 gcggtgattt ttccggaggc gacgtcgatc gccgagaccg aggcatcgcc gccgttcgag
      361 acgaacacga ccttgccgtc ggggcgcacc gcgatgccgt tggcgcgttt gcccgcggcg
      421 atgacggcga tcacctcggc gcgagcgcag tcgatcacgt agacgcggtc ggaattctcc
      481 gcggccacgt aggcgcggct gccgtcggga agaaagccga tgccgcgcgg gcgctccccg
      541 accttgatcg acttgaccgc gctgcgcttc gcgacgtcga tcacgtcgac cgccgcgccg
      601 tcttcggcgc tgacgtacag ccatttgccc tcgggactga acaccacgtg ctcggggttc
      661 tgcccgccga ctctcacgct cgtcgcctcg cgcggcgccg cggtgcggat cagcgaaacg
      721 ctgttgttct cctcgaccgc caccgcgatc cacgcgccgt cgcgcgagat cccgacgccc
      781 tcgggcgatt ttcctaccgc gatccgatcc gagacctcgc gagccgcgag gtccacgacc
      841 acgagcgagc cgcccggctg gtcgctcacg tacagcagcc ggccgtcggg agtcgcggcg
      901 atgccgcgcg gcttgccgcc cgtctcgatg gtcgcgacga cctggtcgct cgccgtgtcg
      961 atcaccgaca ccgtgcccga tctttcgttg ggaacatagg cgaaaggcgc tgcccgcgcc
     1021 tgcgacaggc acgcgagcgc gagggcgagg aggccgattc gtcgaagttt catgtggaat
     1081 ccgaggcgct ggggatcggt cgcaattgta gtgcaatccg gtatcatccg gcggcatcgg
     1141 atatttgcga gtgacgtgaa acctctattg cacgccgggg cagcggtcct gctcatatgg
     1201 ggactcagag ccgaggcgca gttcgtggat ccttcgctcc gctggcgcac gctcgacacc
     1261 gcgcacttct cggtgcattt cgccgatcac taccggtctc aggcgcagtt cgtggcggag
     1321 gtcgccgaat ccgtctatcc gcgcgtcacc ggctggctga actggaaacc ggagtcgcgt
     1381 acgcagatcg tggtgctcga ttcgctcgat ttttcgaacg ggtacgcttc gccgtacccg
     1441 ttcaacttca tcggcatcat cctgtcgcct cctgacgaag gcgagctgct gcagaaccgc
     1501 gagtggctgg agctcgtgct cacgcacgag ttcacccaca tcgttcacct cgaccaggcg
     1561 cggggcccgg cgctcgcctt gcggggcatc ttcggccgct atctcgtgct gccactccct
     1621 ttcacgctgt tctttccgag cggatttcca aacctgctcg aacccaactg gatgatcgaa
     1681 ggcctcgccg tgtattccga gtccgaccgg agcaaggggt acgggcggct cgggcaaagc
     1741 cagttcgagg ggatgatgcg cgccgaaagc gcgcgtggac tgcgctcgct tcgcgaagtg
     1801 aacgccgaag gacgcggctt tccgctcaac cgcgactatc tctacggcag ctatttcttc
     1861 cttttcctcg cagagcgcta cggcccggac gcgatcccca agtatgtgga gatctacagc
     1921 ggccgctggt ttccgttccg cgtgcacagc accccgcggt tcgtcacggg caaagccatg
     1981 gacgagctgt gggtcgaata ccacgactgg ctgaacgcgc gctttgccgt gaagcccagg
     2041 gagcccggca aggagcccga cgggggcgga gagttcctcg tgcgcgactg gtcgattacc
     2101 agtccccttc gcacgtcccg gggagatcgc tggtacgtgc agggaaacgg ctacgtactt
     2161 cccaagctga tgcgtcagac cctgggcggc gaggtcgaac ccgttcgcga catcgagccg
     2221 ggcacgcggc tcgcggcgtt tcccggcggg gagctgctgc tctcccagcc cgagatctgc
     2281 agcaactaca actactacta cgacttgtat cggctcggcc cggacggcgc cttgagccgc
     2341 gtcaccgagt gcgggaggtt ccgttttgcc gccccgctcg acgatggccg catcgtcgcg
     2401 atccgcgtgg tgaacggcga ggcggaagtc gttctgctga attcgcgggg cgggcccgag
     2461 cgctcgcttt accgcgcagc gcctggcgag tcgctcaccg ggctcgccgc caagggcgac
     2521 accgtcgtcg tgacaagcct gcgcaacgaa cgctggtcgc tcgtgcgaat cgccgagggc
     2581 aagtcctcag tgctcgtctc cgaccgtgcg gtcaagcact cgccgcgctt cggcgattct
     2641 gcggacgaga tctatttcgt cgccgactac ggcaacgtga acaacgtctg gtcgtggcgg
     2701 cgcggcgacc gcagtctcgc gcgctggacc gaagcgcgga acggcgtcgg ggaaatcagc
     2761 gcgccggtcg gaggcgaact gctcgtgacg acgatcgagg ccgacggcga cgtgctgcgc
     2821 gcgcgaagcc tcccggatgc gccgctcgaa gttcgccggg cggcggcgga ggccgggcct
     2881 tcggccgcgc cgccggaggg cgcaccgccc ccgggcgggg atcgggctta ctccgcgtgg
     2941 tcttctctcc ggccgcgcta ttggctgccg atcggctacg ccgcggacgg cgccgttgcg
     3001 ctcggcatcg cgaccgacgg ccaggatgcg ctcggactgc accggtacac gtttgcgcct
     3061 ctgtatgaaa tcacccagca ccaggcgctg gggagcgcga cctacgtgta cgacaaccgg
     3121 cacggcctgc tgctcaatcg gttcatgacc gtcaagacgc tcgattccgg cagaaacatt
     3181 gaagcctacg ccatcaacga gacggcgcag tgggtgtccc tgtggcgcca gctttccttg
     3241 ggcacccgca tctactgggg actgggcgcc gcgctcgacc gcgaaatcct gcacgatctc
     3301 gctgcgggga cgtccactcc gcgcgacgag cgcgtgctgg ggctcgtcgc cggagtcgat
     3361 acgcgccgcg agcagtttct ctcggagggc ccgagtcagg ggcagcagct gcggcttttc
     3421 gcggagactt caaatcgtct gcgcggcgcc tatgccggca atttctatcg ggcggactgg
     3481 cgcgcgtatc tgcccgtgta caagtcggtg gtttcgctgc gctggaatga gacctactcc
     3541 cagcccgaag ccgagccggt ccagctgggc ggcagctttt ccgaggaaac cttcgcgctg
     3601 cccgtgctca atcagcgcga gtttcctctg cgcggctacc tcagcggcga agcggtcttg
     3661 acagggcatc gttcgcgcct cggcacgatc gagtggcgca cgccgatatc ggacgtcgac
     3721 cgccacctca tggtgccgcc gctgggcctg aaccggattt cggtgagcgt cttcttcgat
     3781 gccggagccg cttgggaaag cggcgccccg cagcgttact tcaagagcgc gggcgtggag
     3841 ctgttgtcgg aagtgcgcct gggctatcag ctcggcgcgc agctccgggc gggcgtcgcc
     3901 aaggggttcg aatcacccgg aaggacggtt gcgtatttgc acgtcgggcg ctcgttctaa
     3961 agcgcccgcg atgcgtaggc ggttcatcgc atcgggcggc gtaccggcgc cggaacgcct
     4021 caaagcgaga gcttggtctg gaccaggaac tggaacgatt tgatgttcgg atcgcggttc
     4081 agggggaagg caagatcgac gtgcagcacg ttcccgaagg aagagcgcgc gctcaggatg
     4141 cgcaggccga agccgacgtc gttcagccag cgtgcgctcg agggactttc cccggggccg
     4201 ctccacgccc ggcccaagtc gtaatacacc gcgcccccca cgcggaacag gcggaatggg
     4261 taccagtccg tatagacgcg ttcctcgaca ttgaacacca cgcggcggtc gccggtctgg
     4321 tagttccgcg gatatccgcg caggccggtg tcgcccccga gcaccagctg gctggaattg
     4381 gggtccctga ggatgtcggc ggcgagagag acgaacgtca gggtgcggtt gtccgggtgg
     4441 ccatagaatc gcatcgagcc gctcgccagg tagcggtcga gcggcccgta accggtctgc
     4501 ccggagaccg aggtcgagcc cagcaagacc cagttcgacg gaagccgaaa gccgtcgttc
     4561 gcgctggccg agtagagcca gacgttttgg gtcgagccga ggccggtcaa cgagcggccc
     4621 agctgaacgc tcgcctgggt tcccatcgcg aagtactccg gacgctcgat caggtcccgg
     4681 ttcttgacct tctcgtagtt gtcctgcacg agctcgtagc gaaaaaaggg agcgacgagc
     4741 gtctgatcct gaggaaatgg attgggagga gggagaaggt ccgggtcgcc cttataggtt
     4801 tccttcaggt agctcaagcc cgcggaatag cggtgtgtcc agccctcgac caagccctcg
     4861 gaccagccgc cgaatgtctc ggccgtgtcc cgtctgtggc gatcctggaa ctgcttcacc
     4921 gcgtcgttga agaaggagtc gatgcgggtg tccatggaag ccgagaaccc ggccgcccag
     4981 cgcgtgtcca gggcgtagaa cggccggacg atcgatatcg cgttgctctg gccgtcactc
     5041 agctgggagt gcgagtagtc gatcgcggtc caaccgtcaa aagcgtgggg ctgcgagacc
     5101 gtgtacttgg tttcggtgcg atcggcatcg gtggagcgtg tggcagcgat gagaacgccg
     5161 gtgccgaggg cgttggtgtc ccgcagcgtg aacccggtct tgttgacgcc cccagcgcgg
     5221 ctcgcgctcc atccgggctc cagcgtccag gtatcgcgcg tcttgacttc gatatcgacc
     5281 acgccgtcgc ggtaggcgac cggcacgatg ctcacatcgt agaaaatccg gctcgagcgc
     5341 atcaggcgct ccgtctcctc gatgaggcgg accgagacgc gctcgcctgg cttgaacaac
     5401 aactggcgcc tgacgacccc ggcgcgcgtt tgaatgtgta aggcgttcgc ggcgcggtag
     5461 aggatgccgt tttccttctc gtcagcgagg tcgaagatgt cgcgggtggc gatccgcacc
     5521 tcgccgatga ccgctccggc agcctcgagc tcggcgaatg acgggatttt ctgcgtggaa
     5581 ggcggcgtag gctccgcggg gagcggggct ttcggttccg tagcgggttc ctgggccgcc
     5641 gccggcccga gggagcaggc gatcaccgcg cagcagccgc cgagcaaacg ggcgaggacg
     5701 gccgcccgga cccgcgttac tccggtttga ttccggcttc cttgacgacc ttcgcccatt
     5761 tgaccgtctc c
//
LOCUS       DASPZD010000377         4982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_180778, whole genome shotgun sequence.
ACCESSION   DASPZD010000377 DASPZD010000000
VERSION     DASPZD010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180778"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VEM33_07295"
     CDS             <1..918
                     /locus_tag="VEM33_07295"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYS51668.1"
                     /translation="ELQKHLRDGSFVITAELSPPVSTDPAEFIDHALALRGLVTAINV
                     TDGAGSKAHMSSLAAAHFLVRSGVEPVLQITCRDRNRIALQAELLGAAALGVRNILAL
                     RGDDPTLGDQPDAKPVFDLDTTALLATAQRMRSEHRLPSGTEIKGAVRFVLGAAELPV
                     DPPAGWRPDGLLAKMEAGAEFIQTQFCMDPGAVRRYAARLLELGVAQKLPILIGIAPI
                     PSARSARWMKEKLYGAMIPGGLVERLERAADPKAEGRRICVELLQQLAETPGVAGAHV
                     MAPTFHSAIGPVIEASRVAGTRRAGARRL"
     gene            complement(929..1612)
                     /locus_tag="VEM33_07300"
     CDS             complement(929..1612)
                     /locus_tag="VEM33_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HYS51669.1"
                     /translation="MPLERARQKVVSVFAYRCVYLFAALLVLIVMVPFFEESERGRIA
                     LNAVNILILFTAAVAVSHSRVCFALSVALGASCLAFQVASFMLPEAQLTLLLLSRAFG
                     AAFYFLTVSYLLTYVLNREVLTLDKLYGAASAFLMLGILWGYFYAFVLSFYPGALTSG
                     GVPIKVPRISETLYFSFTVLTSTGFGDITPVHPVARMLCVLEQVTGVLFIAILIARLA
                     GTYPPVGRS"
     gene            1747..2661
                     /locus_tag="VEM33_07305"
     CDS             1747..2661
                     /locus_tag="VEM33_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51670.1"
                     /translation="MSVRNLQHIALAVPDPAVGRKFYTDFGMEGKEHDKRVVMRCHGR
                     DQDQVVLVEGKKKHMHHVGFGTKPEDLASLKKRLEQNATKLLDAPKETPYDGVWFRDP
                     DGLLVNVRVAEAAKWRTAAEWKINNPGHLNRAGVPGHPKRDVQVRPTRLGHTLRFTPQ
                     MEKMVDFYTRVVGLQLSDRAQDIVAFMRGHGGSDHHIFGYIKSDRPGFHHASFEVGNV
                     DEIGMGACRLLDKGYKDGWGFGRHVIGSNFFHYIRDPWDSMAEYFCDIDYIPADSNWK
                     ATNYPAEDSLYVWGPQPPEAFGMNFEAG"
     gene            complement(2669..3547)
                     /locus_tag="VEM33_07310"
     CDS             complement(2669..3547)
                     /locus_tag="VEM33_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51671.1"
                     /translation="MSKFVISPHFRLQEWVAEEKGYFKTEGLDYEFRDNYGPGDVGKK
                     HATPNKIGAYQSIEQGRACDVSGACHWTVNVAASTGHGKLYADAYSVAPCGVFVPPDS
                     DIVHPSQLKGVPISVGYQSGSHYSTIQGLEQYLALDDIKLSFADGLLFSRLEKLIDRK
                     VPAASLFSGPYYFLEQLGFRKVIDTTFMMATSITGSPDPEDLRKYFRALRRAQRDIDL
                     RPELYTHYYRKEFPARFLDQMDTRRWGPGERIVFEPYTKEVFEESFKWIAERKIFSEG
                     DMGPGNYEDSVISLAA"
     gene            3695..4363
                     /locus_tag="VEM33_07315"
     CDS             3695..4363
                     /locus_tag="VEM33_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HYS51672.1"
                     /translation="MEFLVGGAPRRIEITTLIIAGWTGRDKTAVEHHIAELAAIGVKP
                     PRVVPCFYRVGANLLTTAGEIEVVGEDSSGEVEFVLVSAPDAMYVGVGSDHTDRKVEA
                     YGVTVSKQMCPKLIGGALWRFAEVEEHWDRLLLRSHVTRKGRRQLYQEGAVSKVLSPR
                     DLLARLADSGGKLSPGTAMFCGTLSVQGEIGGGERFEIELHDPLNNRSLRHGYATRSL
                     TIAD"
     gene            4375..4764
                     /locus_tag="VEM33_07320"
     CDS             4375..4764
                     /locus_tag="VEM33_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HYS51673.1"
                     /translation="MAIQKEHKEFYKVDLDRGWEKLPGYPDGIRQQVLAGFLYEKGKR
                     GFRTRHLRFAAGVHTTEPFVHDYWEEVYLVSGDLIVGNDRNGKGGTRFGPNTYACRPP
                     GTLHGPFKSETGCLLLEMHYYDEAAQR"
     gene            4783..>4982
                     /locus_tag="VEM33_07325"
     CDS             4783..>4982
                     /locus_tag="VEM33_07325"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756770.1"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYS51674.1"
                     /translation="MAEVVLRPIAALAQELAEGRTTSRKLVEAALARIADAGGEGGRV
                     FTKVHAQGALLAADASDHLRMRG"
BASE COUNT          965 a         1568 c         1637 g          812 t
ORIGIN      
        1 gagctgcaaa agcacctgcg ggacggaagc ttcgtcatca ccgccgagct ttccccgcct
       61 gtgtccaccg accccgccga attcatcgat catgcgcttg cgttgcgggg gctcgtcacc
      121 gcgatcaatg tgaccgacgg cgcgggatcg aaagcccaca tgtcaagcct cgcggcggcg
      181 cacttcctgg tgcgaagcgg tgtcgagccc gtcctgcaga tcacctgccg tgaccgcaac
      241 cgcatagcgc tgcaggccga gcttctcggc gctgccgcgc tcggcgtacg taatattctc
      301 gcgctgcgcg gcgacgaccc gacgctcggg gaccagccgg acgcgaagcc cgttttcgac
      361 ctggatacga cggccttgct cgcgaccgcg caacggatgc gctcggagca caggcttccc
      421 tcgggcaccg aaatcaaggg cgccgtgagg ttcgtgctgg gcgcggcgga gctgcccgtg
      481 gatcccccgg ccggctggcg tcccgacggc ctgctcgcga agatggaagc cggcgcagag
      541 ttcatccaga cgcaattctg catggatccc ggcgcggtgc ggcgctacgc cgcgcgattg
      601 ctcgaactgg gcgtggcgca aaagctgccg atcctgatcg gcatcgcccc gattccttcg
      661 gcgcgctcgg cgcgctggat gaaagagaag ctctacgggg cgatgatccc gggcggtctc
      721 gttgagcggc tcgagcgcgc ggccgacccg aaagccgaag gcagaagaat ctgcgtcgag
      781 cttctgcagc agctcgcgga gacaccgggt gtggcgggcg cacacgtgat ggccccgacc
      841 ttccattccg cgatcggccc cgtgatcgag gcctcacgcg tcgccggaac gagacgcgcg
      901 ggcgcgaggc gcctctagcc cggccgactc agctcctacc gacaggcgga taggttcccg
      961 ctaggcgcgc gatcagtatg gcgatgaaga gcacgcccgt gacctgctcc agcacgcaca
     1021 gcatgcgcgc aaccgggtgt accggggtga tgtcgccgaa gcccgtgcta gtaagcacgg
     1081 tgaagctgaa gtagagggtc tcgctgatcc tcggtacctt gatgggtacg ccgcccgagg
     1141 tcagcgcgcc ggggtagaac gagagcacga aggcatagaa gtacccccag agtatgccga
     1201 gcatgagaaa ggcggacgcc gccccgtaaa gcttgtcgag ggtgagcacc tcccggttca
     1261 gcacgtaggt aagcaggtag ctcaccgtca ggaaatagaa cgcggccccg aacgcgcgcg
     1321 agagcaggag cagcgtcagc tgcgcttccg gcagcatgaa agacgcgacc tggaacgcga
     1381 gacacgatgc gcctagcgcc acggaaaggg cgaagcagac gcgcgagtgc gacaccgcca
     1441 ccgccgccgt gaacaggatc aagatgttga cggcattcag cgcgatccgg ccgcgttccg
     1501 attcctcgaa gaacggcacc atgacgatca gcaccagcag cgccgcgaac aggtagacgc
     1561 agcggtaggc aaataccgac accacttttt gtcgcgcgcg ttcgagcggc attccagatc
     1621 tcctcaacgc cggcgccgga gggcattata ttcgcgggcg gaggaaaacc agtgggacgc
     1681 cgcccaatcg tcttcgccgg cgtttggatt cataattacg aaagtccgaa aaggaggaaa
     1741 ccgtcaatga gcgtccgcaa cctgcagcat atcgcgctcg ccgttcccga tcccgcggtc
     1801 ggcaggaagt tctacaccga tttcgggatg gagggaaaag agcacgacaa acgcgtcgtc
     1861 atgcgctgcc acggccggga ccaggatcag gtcgtgctgg tcgaaggcaa gaagaagcac
     1921 atgcaccacg tcggcttcgg caccaagccg gaggatctgg cgagcctgaa gaagcgactc
     1981 gagcagaacg ccaccaagct cctcgacgcg cccaaggaaa ccccctacga cggcgtctgg
     2041 ttccgcgacc ccgatggact gctcgtaaac gtacgcgtcg cggaagccgc gaagtggcga
     2101 accgccgcgg agtggaagat caacaacccg ggccacctga accgcgccgg cgtgcccggc
     2161 cacccgaagc gcgacgtcca ggtgcgcccg acgcgcctgg gccacacgct gcgcttcacg
     2221 ccccagatgg agaagatggt ggacttctat acgcgcgtcg tgggcctcca gctctcggac
     2281 cgcgcgcagg acatcgtcgc cttcatgcgc ggccacggag ggagcgatca tcacatcttc
     2341 ggctacatca agtcggaccg gccgggcttc caccacgcga gcttcgaggt cggcaacgtc
     2401 gacgagatcg gcatgggcgc gtgcaggctg ctcgacaagg gctacaagga cggctggggg
     2461 ttcggccgcc acgtgatcgg ctcgaacttc ttccactaca tccgcgatcc ctgggacagc
     2521 atggcggagt acttctgcga catcgactac atcccggcgg attcgaactg gaaggcgacc
     2581 aactacccgg cggaagactc tctctacgtt tggggtcccc agcccccgga ggcctttggc
     2641 atgaatttcg aagcgggcta gaagcttctt aggccgcgag cgagatgact gaatcctcgt
     2701 agttgcccgg ccccatgtcg ccttcggaga agatcttgcg ctcggcgatc cacttgaagg
     2761 actcttcgaa cacttccttc gtgtaaggct cgaagacaat gcgctcgccg ggcccccaac
     2821 gacgcgtgtc catctggtcg aggaagcgcg ccgggaattc ttttctgtag tagtgcgtgt
     2881 agagctccgg gcgcaggtcg atgtcgcgct gcgcccgccg cagggcgcgg aagtacttgc
     2941 gcaggtcctc aggatcgggg ctgccggtga tcgatgtcgc catcatgaaa gtcgtgtcga
     3001 tcaccttgcg gaagccgagc tgctcgagga agtagtacgg cccgctgaag agggacgcgg
     3061 cgggcacctt gcggtcgatc agcttctcca ggcgggaaaa gagcaatccg tcggcgaacg
     3121 agagcttgat gtcgtcgagc gcgaggtatt gctcgagtcc ctggatcgtc gagtagtggc
     3181 tgccggactg gtagccgacc gagatcggca cgcccttcag ctggctcgga tggacgatgt
     3241 cggagtcggg cgggacgaac acgccgcagg gcgcgaccga gtaggcgtcg gcgtagagct
     3301 tgccgtggcc ggtcgaggcg gcgacgttca cggtccagtg acaggcgccg ctcacgtcgc
     3361 aggcccggcc ctgctcgatg ctctggtagg cgccgatctt gttcggcgtc gcgtgctttt
     3421 tccccacgtc gccggggccg tagttgtcgc gaaattcgta gtcgaggcct tcggtcttga
     3481 agtagccctt ttcctcggcc acccattcct gcagcctgaa gtgcggcgaa atcacgaact
     3541 ttgacatcga cggctcctcg gaggtctgtc gggtgaaaaa tctactccga cgaacccagg
     3601 attgcaaagg aaacttgctg tgggatgggt cccattcgct tgcggaacct cgaggccctg
     3661 taaaatagcg gtcgcaaagg gagtgcgaca tcgcatggaa ttcctggttg gcggcgctcc
     3721 gcgccgcatc gagatcacca ctctcatcat cgccggatgg accgggcgcg acaaaaccgc
     3781 ggtggaacac catatcgccg aactggcggc gatcggggtg aagcccccgc gcgtcgtgcc
     3841 ttgcttctac cgcgtcggcg caaacctgct caccacggcg ggcgaaattg aagtcgtcgg
     3901 ggaagactcg agcggagaag tcgagttcgt gctggtttct gctcccgacg cgatgtacgt
     3961 cggggtcggc tccgatcata ccgaccggaa agtcgaagcc tacggagtca ccgtgtccaa
     4021 gcagatgtgt ccgaagctga tcggaggggc gctttggcgc ttcgccgaag tcgaggagca
     4081 ttgggaccgg ctcctgctgc gctcgcacgt gacgcgcaag ggcaggcgac agctctacca
     4141 ggaaggagcg gtgagcaagg tgctttcgcc gcgcgatctg ctcgcgcgcc tggccgattc
     4201 gggaggcaag ctttcgccgg ggaccgcgat gttctgcggt actctttcgg tgcagggcga
     4261 gatcggcgga ggcgagcgct tcgagatcga gctgcacgac ccgctgaaca atcgcagcct
     4321 caggcacggg tacgcaaccc gcagcctcac gatcgcggac tagcaggaga cgacatggca
     4381 atccagaaag agcacaagga gttctacaaa gtcgacctcg acaggggttg ggaaaaactg
     4441 ccaggttatc cggacgggat ccggcagcag gttcttgcgg gatttctcta tgagaaaggc
     4501 aagcgaggtt tccgcacgcg tcatctgcgc ttcgccgccg gcgtgcatac gaccgagccc
     4561 ttcgtgcacg actactggga agaggtgtat ctcgtctcgg gcgatctgat cgtcggcaac
     4621 gacaggaacg gcaaaggcgg cacccgtttc ggtcccaata cgtatgcttg ccgcccgccg
     4681 ggaacgctcc acgggccgtt caagtcggaa acgggttgcc ttctgctcga gatgcattac
     4741 tacgacgagg ccgctcagcg ctgaccgccg aggcaaagcc cgatggccga agtcgtcctc
     4801 cgcccgatcg ccgcgctcgc gcaggagctt gccgaaggca ggaccacgag ccgcaagctc
     4861 gtcgaggcgg cgctcgcgcg catcgccgac gcgggcggcg agggcgggcg cgttttcacc
     4921 aaagtgcacg ctcagggcgc gctactcgcc gccgatgcct ccgaccacct gcgaatgcgc
     4981 gg
//
LOCUS       DASPZD010000378         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1244726, whole genome shotgun sequence.
ACCESSION   DASPZD010000378 DASPZD010000000
VERSION     DASPZD010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244726"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VEM33_07330"
     CDS             complement(<1..334)
                     /locus_tag="VEM33_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HYS51675.1"
                     /translation="MARTGIGKTALSLAVAGAFAGGVSQAHAAAFALIEQSASGLGNS
                     YAGAAAAAEDASTIFYNPAGMSLLPPGMQISAGAALINLSAKFSDSGSQPSGTALNGV
                     NPAAGRPLG"
     gene            complement(465..1685)
                     /gene="tyrS"
                     /locus_tag="VEM33_07335"
     CDS             complement(465..1685)
                     /gene="tyrS"
                     /locus_tag="VEM33_07335"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HYS51676.1"
                     /translation="MSDIDRQLRVIKRGCDELIVEAELREKLKSGRPLRVKLGLDPTA
                     PDLHLGHTVVINKLRQFQDLGHHVQFLIGDFTGMIGDPSGKNATRPPLSREQILRNAK
                     TYQDQVFKILDANKTEVLFNSRWSDELGAAGLIGLAGKWTVARMLERDDFGKRYAAHQ
                     PIAIHEFLYPLMQGYDSVAMKSDIELGGTDQKFNLLVGRELQKHYGQPQQCILTMPLL
                     EGIRGVDKMSKSLVNYVGISESPEAIFGKLMSISDTLMWRYLELLSFESLETLAKWKK
                     EVSGGRNPRDIKVLLAREIVTRFHSAGAAERAQADFEARFKHGATPEGIPEITVRVHG
                     KSTPGKVPEMAITKVLKESQLVPSASEALRMIEQGGVRIDGEKVTDKGLSVKRGETVV
                     LQVGKRKFARVTLA"
     gene            1844..3049
                     /locus_tag="VEM33_07340"
     CDS             1844..3049
                     /locus_tag="VEM33_07340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYS51677.1"
                     /translation="MAAFGTVARVPEPVPVRTVVETLALSATPVGDSGTIIYFQEDRF
                     RRNDTLATLITRLGADGEEAATLLRSSRAARPFHLLLPGTPVQAKVDEVGRLRSISFL
                     TDEDTLLSADRLGDGFGRFERQADVARGIEMKSGEIETSLFAATDAAGISDSIAVQLA
                     DIFGGDVDFHRDLRRGDRFGVVFETFHHAGKTIRFGRVLAAEFYRQRKTYRAVWFQDP
                     WGRGGYYTPDGENLRKVFLRSPLEFSRITSGFGLRRHPIFHQWRAHQGVDYGAPVGTR
                     VKSTGDGVVSFAGRRNGYGNLIVLRHYGGYSTYYAHLRNFAPRLRAGSRVVQGEIIGY
                     VGQTGWASGPHLHYEFHLRDQNRNPLTMAFPAAQPIPAIERAAFRRTADPAIARLDLL
                     KNGDLALLE"
     gene            3094..>3730
                     /locus_tag="VEM33_07345"
     CDS             3094..>3730
                     /locus_tag="VEM33_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HYS51678.1"
                     /translation="MSGTSLDGVDSVLVDLQSRPLLLGAGFLPYPKTLKGTLLALHER
                     SGDELHRAALAGIELSRLYAKAAERLLEKHGVNAQAVSAIGCHGQTVRHRPGSGYSLQ
                     LGNPAWLAERTGITVVADFRSRDIAAGGQGAPLVPAFHAAVFRDSSHHRVIVNLGGIA
                     NLTDLPREGPVTGFDTGPGNVLLDAWMRERFGHDFDRGGALAARGRPVASLL"
BASE COUNT          658 a         1187 c         1139 g          746 t
ORIGIN      
        1 cccccaatgg tcggccggcc gcaggattta ctccgtttag ggctgtccct gacggttggg
       61 aacccgaatc gctgaatttc gcggaaaggt tgatcaatgc ggcgccagcg gagatttgca
      121 tccctggtgg caacaaactc atgccggcgg ggttgtagaa tatggtgctc gcatcctctg
      181 cggccgccgc ggctcctgca tacgagttac ccagcccgct tgcgctctgc tcgatcaggg
      241 cgaacgcggc agcgtgcgct tgcgaaacgc ccccggcgaa agccccagcc accgcgagcg
      301 aaagggccgt ttttccgatt ccggtacgtg ccatgagaaa tctcctcttg atgcgttatt
      361 ggagccgaat tagcaactgt cttggccgcc gacaagatag cacaacgtca tagtctcgca
      421 aatggggcgc tgcgaaggcg agtccccccg cgcgcatacc ggcgtcaggc gagggtaacc
      481 cgcgcgaact tgcgtttgcc gacctgcaac acgaccgttt ccccccgctt cacggaaagc
      541 cccttgtcgg tgaccttctc gccgtcgatg cgcacgccgc cctgctcgat catgcgcagg
      601 gcctccgacg cgctggggac cagctgcgac tccttgagta ccttcgtgat cgccatctcg
      661 ggaaccttgc ccggtgtcga tttaccgtgg accctgacag taatttcagg aatgccctcg
      721 ggcgtcgcgc cgtgcttgaa acgcgcctcg aaatcagcct gggcgcgctc cgccgccccg
      781 gcgctgtgaa aacgagtgac gatctcgcgc gcgaggagca ccttgatgtc gcgcgggttg
      841 cgaccgcccg atacctcctt cttccacttc gcaagtgttt ccagggattc gaaggagagc
      901 agttccaagt aacgccacat cagcgtgtcg gagatcgaca tcaactttcc gaagatcgct
      961 tccggagatt cgctgatacc aacgtagttg acgagtgact tcgacatctt gtcgacgccg
     1021 cgtatccctt cgagaagcgg catcgtcagg atgcattgct gtggctgacc gtagtgcttc
     1081 tgcagctcgc gccctaccag cagattgaac ttctggtcgg tccccccgag ctcgatgtcg
     1141 gatttcattg ccactgaatc gtagccctgc atgagcgggt agagaaactc gtggatggcg
     1201 atgggctggt gggccgcata acgtttgccg aaatcgtcgc gctcgagcat gcgcgcgacc
     1261 gtccatttcc cggccaaccc tataagaccg gctgccccca gctcgtcgga ccacctggaa
     1321 ttgaacagga cttcagtctt gttggcgtcc aggatcttga aaacctgatc ttgataggtt
     1381 ttggcgttcc gcaggatctg ctcccgcgaa agcggcggtc gcgtcgcgtt cttgccgctc
     1441 gggtcaccga tcatccccgt gaaatctccg atgaggaact ggacgtgatg gcccaagtcc
     1501 tgaaactggc gcagcttgtt gatgaccacc gtgtgcccga gatgcaggtc aggcgcagtc
     1561 gggtccagtc ctagcttcac gcgcagtggc cgcccggatt tgagcttttc ccgcaactcg
     1621 gcctcgacga tcagttcatc gcagccgcgc ttgatgactc tcagttggcg atcgatgtcg
     1681 ctcatgggtg ctcagatggc tgaccgggcg gttatttgtt agactttttt gattcaatcc
     1741 gctgccgatc atgcacaacg acgaggctgg aattgtaacg cgctccggcg gaaggcgcct
     1801 ccgacggctg gcggccgccg ttgccgcgcc gctactcggc gtcgtggccg cgttcggcac
     1861 cgtcgcgcgc gtcccggagc cggttcccgt ccggaccgtc gtcgagacgc tcgccctgtc
     1921 cgcgaccccg gtcggcgact cgggaacgat catctacttt caggaagatc gcttcagacg
     1981 caatgacacg ctggctactc tgattacacg gctcggggcc gatggggaag aagcggcgac
     2041 gctcctgcgg tcttcgcgtg cggcgcggcc gttccacctt ctgcttcccg gaacgcccgt
     2101 ccaggcgaaa gtcgacgagg tcggcaggct gcgctcgata tcgttcctca ccgacgagga
     2161 cacgctcctc tctgcggatc gcctcggcga cggattcggc agattcgagc ggcaagccga
     2221 tgtagcgcgg ggaatagaga tgaaatcggg cgagatcgag acctccctct tcgcggcgac
     2281 cgatgccgcg gggatttcgg acagcattgc ggtccaactt gcggacatct ttggcggcga
     2341 cgtggatttt catcgcgatc tgcgccgtgg ggaccgtttt ggggtcgttt tcgaaacctt
     2401 tcaccacgcc ggcaaaacga tccgattcgg gcgggtgctc gcggccgaat tctaccgtca
     2461 gaggaaaaca taccgcgccg tatggtttca ggatccctgg ggaaggggcg gctattacac
     2521 cccggacggc gagaacctgc gcaaggtatt cctgcgctcg cctctcgaat tctcgcgtat
     2581 cacctccggg ttcggcctgc gccggcatcc gattttccac cagtggcgcg cgcaccaggg
     2641 agttgactat ggagcccccg tcggaacgag ggtgaagtcg accggcgacg gtgtcgtgtc
     2701 gttcgcggga cgccgcaacg ggtacggaaa cctcatcgtg ctcaggcact acgggggata
     2761 cagcacgtac tacgcgcacc tgagaaattt cgcgccgcgg ttgagggcgg gcagccgcgt
     2821 cgtccaaggc gaaatcatcg gctacgttgg gcaaaccggc tgggcgagcg gcccccacct
     2881 ccattacgaa tttcacctgc gcgaccagaa ccgcaatccg ctgaccatgg ctttcccggc
     2941 ggcgcagccg atccccgcga tcgaacgggc cgcgttcagg cgtaccgccg atccggccat
     3001 cgcgcgactc gatcttctga aaaacggcga cctagcgctg ctcgagtgag cggttccacg
     3061 agatcggcat cttccgagat ctacatcggt ttgatgtcgg gcacgagcct cgacggcgtc
     3121 gattccgtcc tcgtcgacct gcagagccga ccccttcttt tgggtgccgg gttcctgccg
     3181 tatccgaaga ccttgaaagg caccttgctc gccctgcacg agaggtccgg cgacgagctg
     3241 catcgtgcag cccttgcagg gatcgaactc tcgaggcttt acgctaaggc ggccgaacgc
     3301 ctccttgaga aacacggcgt caacgcgcaa gccgtatccg ccatcggttg tcacgggcag
     3361 acagtgcgtc accgcccggg atccgggtac agcctccagc ttggtaatcc tgcgtggctt
     3421 gcggagcgca cgggcattac ggtggtcgcc gatttcagga gccgcgacat cgctgcgggc
     3481 gggcagggtg caccgctcgt acctgcgttt catgcggctg ttttccgaga ctcgagccac
     3541 caccgcgtca tcgtcaatct cggcggaata gccaatttga ccgatcttcc ccgcgaggga
     3601 cccgtaacag gcttcgacac gggacccggc aacgttctgc tcgacgcctg gatgcgtgag
     3661 cgattcggcc acgatttcga tcgcggcggg gcgcttgccg ctcggggacg gccggttgca
     3721 tccttgctga
//
LOCUS       DASPZD010000379         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1118621, whole genome shotgun sequence.
ACCESSION   DASPZD010000379 DASPZD010000000
VERSION     DASPZD010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118621"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /locus_tag="VEM33_07350"
     CDS             complement(<1..2273)
                     /locus_tag="VEM33_07350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYS51679.1"
                     /translation="MKKSRSLGPAEVDHRKSLSLLRATLESTADGVLVVDNLGKISTY
                     NQQFAEMWRLPPQVLSSGKDEDALNFVLEQLKDPGQFRDKVNALYSHPGQESFDTIEF
                     KDGRIFERYSRPQLVEGQTIGRVWSFRDVTEHRRAEEKLRESEERFRLIAENVGDLVA
                     MVDTEGRRIYNSPSYRTVFKDDEIQPDTDSFREIHPEDRERVRAIFRETVETGVGRRT
                     EFRFVLRDGGIRYIESEGRVIRGAAGKVTKVVVVSRDISERKRAEQRERMEHAVTRVL
                     AESETLAEAIPKIVQTICETLNWDCGARWSMDERKKAVACVETWSIPEKPFTDFIAEV
                     REMTFQPTHRGLVRQVWMSGTPHWIPDVSRDEGFQRAQLAAKAGLHGAFAFPVLAGNV
                     TLAVLEFFSREIRNPDPSLLQMVRVIGSQIGQFMARKQAEENLLYVATHDTLTGLPNR
                     YMFNQRFAHALNNAQRYRKTMALLFLDLDRFKFINDTLGHPFGDRLLQEAAARLRMCL
                     RESDTIARFGGDEFVALIEDFSEPGDVISVAQKILHALRWPFMLQGETCHLSASIGIS
                     LYPNDGADFANLLKNADIATYRAKEQGKNNYLFYSGEMNDHLSARIAKETRLQGALER
                     NEFILHYEPKVEINTGRITGMEALIRWQHPDLGLLPPVEFIGHAEDSGLIVPIGAWVL
                     RIGCAYNQTLQSRVATPLTVSVNLSARQFEDKHLLREIERALSESSLKPNCLELEITE
                     SMVMRDTQSSKKILDGI"
     gene            2414..3292
                     /locus_tag="VEM33_07355"
     CDS             2414..3292
                     /locus_tag="VEM33_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51680.1"
                     /translation="MKLRSIELEVPDRESAVRFFRDTWGLLEAGERNGTSYLRGTEGI
                     PYVVSVARAGEPAVGAVTFSGSKPELARIRKRAAEAGARLGPSRDFDEPGGGSGFFVE
                     GPEGHVFRLVTERKRVKRLRPDHDRPLQVTHVVLNARDREACTRFAVEVLGFRVSDRT
                     GFMNFVRCDRVHHAIAYAQSDNSMLNHIAFEMTDIDAVMRGIGRLKDAGIETFWGPGR
                     HGPGNNVFAYFVAPFGAAVEYTSEIQRVGSSYRTGKPEDWKWPPNRNDHWGVSARNAA
                     AISQAERQFRFRPPAA"
     gene            3311..3835
                     /locus_tag="VEM33_07360"
     CDS             3311..3835
                     /locus_tag="VEM33_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS51681.1"
                     /translation="MVQPTRRVVTGHDAKEKAVVLIDAAAPNAKLRKATGLVSTLLWV
                     TEESPADNSGSADAAEREIGVAPPPRGSIFRVVDFPPAADFGAVDNAAMLREMRIEAG
                     KGDARHASMHRTKSIDYAVVISGEIDMLLDDSEVHLKAGDVLVQRGTNHGWVNRGREN
                     CRVAFVLIDAEEPA"
BASE COUNT          650 a         1237 c         1292 g          735 t
ORIGIN      
        1 ttgatcccgt cgagtatttt cttggagctc tgcgtgtcgc gcatcaccat gctctcggtg
       61 atctcgagct cgaggcagtt cggtttgagc gagctctcgg agagcgcccg ctcgatctcc
      121 cgaagcaggt gcttgtcctc gaactgccgc gccgacaggt ttaccgacac cgtcagcggg
      181 gtcgcaacgc ggctctgtag ggtctggttg tacgcgcacc cgatcctgag cacccaggcc
      241 ccgatcggga cgatcagccc cgagtcctcc gcgtgcccga tgaactccac cggaggaagc
      301 agtcccaggt cggggtgttg ccagcggatc agcgcctcca tgcccgtgat ccggccggta
      361 ttgatctcga ccttgggttc gtagtggagg atgaactcgt tccgctccag ggcgccctgg
      421 agccgggtct ccttggcaat cctggcggac aggtgatcgt tcatttcccc cgaatagaac
      481 aggtaattgt tcttgccctg ctccttggcg cggtaggtgg cgatgtcggc gttcttcaac
      541 agattcgcga agtccgcgcc gtcgttcggg tacaggctga ttccgatgct cgcgctcagg
      601 tggcaggtct cgccctggag catgaaaggc cagcgcaggg catggaggat tttctgggcc
      661 acgctgatca cgtcgccggg ctcgctgaag tcctcgatga gcgcgacgaa ttcgtcgccg
      721 ccgaaacggg cgatggtatc gctttccctc aggcacattc tcagacgcgc cgcagcctcc
      781 tgcagcaggc ggtcgccgaa cgggtggccg agcgtatcgt tgatgaactt gaagcgatcg
      841 agatcgagga agagcagggc catcgtcttg cggtagcgct gcgcgttgtt gagcgcgtgg
      901 gcgaagcgct ggttgaacat gtagcggttg ggcaatcccg tcagggtgtc gtgcgtcgcg
      961 acgtagagga ggttctcctc ggcctgcttg cgggccatga actggccgat ctggctgccg
     1021 atcacgcgca ccatctgcag gagcgacggg tcgggattgc ggatctcgcg gctgaagaat
     1081 tccagcacgg cgagggtgac gttgcccgcg aggaccggga aggcgaacgc gccgtgcagc
     1141 cccgccttgg cggcgagctg cgcccgctgg aagccctcgt cgcggctcac gtccggtatc
     1201 cagtgcggcg tgccgctcat ccacacctgc ctcaccagcc cgcgatgggt cggctggaac
     1261 gtcatctcgc gcacttcggc gataaaatcg gtgaagggct tctcggggat gctccaggtc
     1321 tcgacgcagg cgacggcttt tttccgttcg tccatgctcc agcgcgcccc gcagtcccag
     1381 ttgagcgtct cgcagatggt ctggacgatt ttcgggatag cctcggcgag cgtttccgat
     1441 tcggcgagca cgcgcgtcac ggcgtgctcc atcctctcgc gctgctcggc gcgcttgcgc
     1501 tcggagatat cgcgcgagac gacgaccacc ttcgttacct tgccggccgc gccccggatg
     1561 actcggcctt ccgactcgat gtagcggatg ccgccgtccc tgagcacgaa gcggaactcg
     1621 gtccgccgcc ccacgccagt ttcgaccgtc tcgcggaaga tcgccctgac gcgctcgcga
     1681 tcctcggggt ggatctccct gaatgaatcg gtgtccggct ggatctcgtc gtccttgaaa
     1741 acggtgcggt aggatgggct gttgtagatg cgccgcccct cggtatcgac catggcgacc
     1801 agatcgccga cgttctcggc gatgaggcga aagcgctctt cgctctcgcg cagcttttcc
     1861 tcggcgcgcc ggtgctcggt cacgtcgcgg aagctccaca ccctgccgat ggtctgcccc
     1921 tcgacgagct ggggcctgga atagcgctcg aagatgcggc cgtccttgaa ctcgatggtg
     1981 tcgaaactct cctggccggg atgcgaatag agcgcgttca ccttgtcgcg gaactggccg
     2041 gggtctttca gctgctcgag gacgaaattg agggcgtcct cgtccttccc ggaggacagg
     2101 acctgcggcg ggaggcgcca catctcagcg aactgctggt tgtaggtgct gatcttgccg
     2161 agattgtcga cgaccaggac gccgtcggcc gtggactcga gcgtcgcacg gaggagcgag
     2221 agcgatttcc ggtggtcgac ctcggccggc ccgaggctcc tgctcttttt catttgcctc
     2281 cctttttcta gtcgccgggt ttgggattgt atccggattt ggtgcccggg caagtacttc
     2341 ccgaagcgaa gggttacaat tgttcgcaca cggctgtcga ttcgaacacc gcgcaccggt
     2401 ccggagcgaa aacatgaagc tgcgcagcat cgaactcgaa gtgcccgacc gcgaatcggc
     2461 tgtccggttc ttccgcgaca cctggggcct gctggaggcc ggcgagcgga acgggacgtc
     2521 ctatcttcgg ggcaccgaag gtattcctta tgtcgtctcg gttgcgcggg cgggcgagcc
     2581 cgccgtgggc gcagtcactt tctccggctc gaagcccgag ctagcgagaa tccgcaagcg
     2641 cgccgcagag gcgggggcgc ggctcggtcc ttcccgcgac ttcgacgaac ctggcggcgg
     2701 gtcgggattt ttcgtcgaag gtcccgaagg ccacgtgttc cggctcgtca ccgagagaaa
     2761 acgcgtgaaa aggctgcgcc cggaccacga caggccgctg caggtgacgc acgtcgtcct
     2821 caacgcgcgg gaccgggaag cctgcacgcg cttcgcggtg gaggtcctcg gttttcgcgt
     2881 ttcggatcgg actggcttta tgaatttcgt gcgctgcgac cgcgtccatc acgcaatcgc
     2941 ctacgcgcag tcggacaatt ccatgctcaa ccacatcgct ttcgaaatga ccgacatcga
     3001 tgctgtcatg cgcggcatcg gaaggctgaa agacgccgga atcgagacat tctggggccc
     3061 cggacgtcac gggcccggca acaatgtctt cgcgtatttc gtcgcgccgt tcggcgcagc
     3121 ggtggagtac acctccgaaa tccagcgcgt cggcagctcg tacaggaccg gaaagcccga
     3181 agactggaaa tggcccccca accgcaacga tcactggggc gtgtcggcgc gaaacgccgc
     3241 cgcgatatcg caggccgagc ggcaattccg cttccggccg cccgccgcct gagcctcttc
     3301 cgctgaaggc atggtgcagc cgacgaggcg cgtggtcacc ggccacgatg cgaaggaaaa
     3361 ggcggtcgtc ctgatcgacg ccgccgcgcc gaatgcgaag ctcagaaaag cgacaggcct
     3421 cgtctctacg ctcctgtggg tgactgagga atcgccggcc gacaattccg gaagcgcgga
     3481 cgctgccgag cgcgagatcg gcgtcgctcc tccgccccgc ggctcgatct tccgcgtcgt
     3541 cgactttccg cccgccgcgg atttcggtgc cgtcgacaac gcggccatgc tgcgcgaaat
     3601 gaggatagag gccggcaagg gcgatgcgcg ccacgcgagc atgcaccgca cgaagagcat
     3661 cgactacgcc gtcgtgatct cgggcgagat cgacatgctt ctcgacgatt ccgaagtgca
     3721 tctgaaagcg ggagacgtgc tggtgcagcg cgggacgaac cacggctggg tgaatcgcgg
     3781 gcgggaaaac tgccgcgtcg ccttcgtgct gatagatgcg gaggagccgg cgtagcgctc
     3841 aggtgccgcc agtcgcgggc aagagcagat acgcgcacca tgcggggctt cgcggtcacg
     3901 gcagcggctt cgcg
//
LOCUS       DASPZD010000380         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1260674, whole genome shotgun sequence.
ACCESSION   DASPZD010000380 DASPZD010000000
VERSION     DASPZD010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260674"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VEM33_07365"
     CDS             <1..507
                     /locus_tag="VEM33_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51682.1"
                     /translation="VTDSPGLFASGDGGHPHCITIGNDDLVYVCDRADDRIQVFTKTG
                     ALQRIIPVVPGTGVTLGIGGASGLGTAGSAWDLAFSNDDRQAFMFEADGGNEILHIMD
                     RAMGTILSGFGAPGLQPGQFTFLHSVALGSKGNLYTGETINGRRIQKFTRVDCNNGRG
                     RGNDDCDD"
     gene            715..1977
                     /locus_tag="VEM33_07370"
     CDS             715..1977
                     /locus_tag="VEM33_07370"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HYS51683.1"
                     /translation="MHAHEFKLDSVINAFVRIEPGQAELVVRAPLYLFKSVKFPVTGA
                     EIDVDNSAPAVERALAAIQKDIMLFENDRALVASHAMGRLSLPSDRSFETYEQAANHV
                     GEPLERGTSIYIDQGYVDARITYPIGSPGSEFALRTTAGPELGDALKLALRYMPLGED
                     SRAMVVTGRSGTVALNPNWVNAATGFTGLGIAHILTGYDHLLFLLCLVIPLRGWRQIL
                     SVITVFTVAHSFTLLGSAFNLAPSGKWFPPFVETAIAASIVYMALENIMGVNLERRVL
                     ITGLFGLVHGFGFSYGLQENFQFAGTHLLVSLFAFNVGIELGQIMVLAVMLPALAVVR
                     RYVLPGRVGMIILSTIMADTGWHWMIDRADVLWKTPWPRPSAAGLAILALWVAGILLA
                     AGGLSFIAKRLRFTPGDGLPSPQRGVAD"
     gene            complement(1994..2944)
                     /locus_tag="VEM33_07375"
     CDS             complement(1994..2944)
                     /locus_tag="VEM33_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51684.1"
                     /translation="MARSAGVVAILLSLSALAQYPSKPVRLVVTFPPGGITDFTARAL
                     GPRLSEALGQPVVVENKTGSGGNIGTDFVAKAPPDGHTLLIAAPPNAINVSLYRSLPF
                     DTKKDLAPVALVGSGANVLVVSAKTPVGSVRGLLEHARRNPGKLNFASNGNGTSIHLS
                     GELLKYYGKFDAVHVPFRGAPAAMAALLAGDVDFMFDALSISAPQIKAGKLKLLAVTS
                     RERSPMFPDAPTLVESGFPEFEVSGWTGVMTTGGTPAAVVARLETELRRILSDRELIA
                     AFEKPGMTVSFAGAREFARFLDEEIARWAIAVKHSGAQAD"
     gene            complement(2971..>3476)
                     /locus_tag="VEM33_07380"
     CDS             complement(2971..>3476)
                     /locus_tag="VEM33_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51685.1"
                     /translation="YRKACADLGRPAPAPDRFSYLGIVAIAQDRKEAMRRAEVMVGYL
                     RSSGRVAEPFGTPPGYFSVKDAARMIKARGKRTTTTREGKAVDTHHGAIEDLVKAGIM
                     FVGDPDDVYRQLAEFVDNVGGLGHFLMMGHAGHMTHAEAVDQITLVAKEVLPRFKKPR
                     AASKQAA"
BASE COUNT          578 a         1121 c         1146 g          631 t
ORIGIN      
        1 gtgaccgact cgcccggctt gtttgcctcg ggcgacggcg gccatccgca ttgcatcacc
       61 ataggcaatg acgacctggt gtatgtgtgc gatcgcgccg acgaccgcat ccaggtcttc
      121 accaagaccg gagcgctgca acggatcatt ccggtcgtcc cgggtaccgg cgtgacgctg
      181 ggaatcggcg gcgcatcggg gctcggcacc gcgggctcgg cgtgggacct cgcgttctca
      241 aacgacgaca ggcaggcgtt catgttcgag gccgacggag ggaacgagat cctgcacatc
      301 atggaccgcg cgatgggcac gatcctttcc gggtttggcg cgcctggact ccagccgggc
      361 cagttcacgt tcctgcacag cgtcgcgctc ggctcgaagg gcaacctcta caccggcgag
      421 acgatcaatg gccggcgcat ccagaagttc acgcgagtcg actgcaacaa cggcaggggt
      481 agaggcaacg acgattgcga cgactgatgt gaggcgcggc cggaagcgac agcccctggc
      541 tgctccgctt gcaggttcgc atacccgcgt cacccggcat catcaccggt tgacgcgggt
      601 tcgttttggc aagccgccgg ctcaatatct gcagcgagaa gtgagatgaa agttcgcggt
      661 ttacgactcg cggcatggag tgtccttgcc tgtgctgctg gctacacgca gatcgtgcac
      721 gcgcacgaat tcaagcttga ctcggtcatc aacgcattcg tcaggatcga accgggccag
      781 gccgaactcg tcgtccgcgc gccgctgtat ctgttcaagt cggtcaagtt tccggtcaca
      841 ggcgccgaaa tcgacgtcga caactcggcg ccggcggtcg agcgagcgct cgccgcaatc
      901 cagaaggaca tcatgctctt cgaaaatgat cgggcgctgg tcgcatcgca cgcgatgggg
      961 cgattgtcgc tgccgtccga tcgttccttc gaaacctacg agcaagccgc gaaccacgtg
     1021 ggcgagccgc tcgaacgcgg cacgagcatt tacatcgacc agggttacgt ggacgcccgc
     1081 atcacctatc cgatcggctc gccgggatcg gagttcgctc tccgcacgac cgccggtccg
     1141 gagctcgggg atgccctcaa gctcgcgcta cgctacatgc cgctcgggga agacagccga
     1201 gcgatggttg ttaccggccg ctcgggaacg gtcgcgctca acccgaactg ggtgaacgcc
     1261 gcgacgggct tcaccggcct cggcatcgcg cacatcctca ccggctacga tcatctgctt
     1321 ttcctgcttt gcctggtgat tccactgcgt ggctggcggc agatcctgtc ggtgatcacg
     1381 gttttcacgg tcgcgcattc gttcacgctg ctgggctccg ccttcaatct cgcgccatcg
     1441 ggcaaatggt tcccaccttt cgtcgagacg gcaatcgccg catcgatcgt ctacatggcg
     1501 ctcgagaaca tcatgggcgt taacctcgag cggcgcgtcc tgatcacggg cctgttcgga
     1561 ctggtccacg gcttcggctt ctcgtatggc ctccaggaga atttccagtt cgccggaacc
     1621 cacctgcttg tttcactttt cgcgttcaac gtaggcatcg aactgggcca gatcatggtc
     1681 cttgccgtca tgctgccggc gcttgccgtg gtgcggcgat acgtgttgcc cggccgggtc
     1741 gggatgatca tcctctcgac gatcatggcg gacaccggtt ggcactggat gatcgaccgc
     1801 gcggacgtat tgtggaaaac gccgtggccc cggccgagcg cggccggcct tgcaattctg
     1861 gccctctggg tggccggcat actgcttgcg gctggcgggc tcagcttcat cgccaagcgg
     1921 ctgcggttca caccgggcga cgggctgccg tcgccgcaga ggggcgtggc ggactagact
     1981 ttcatagtcc gactcaatcg gcctgcgcgc cggagtgctt caccgcgatc gcccagcggg
     2041 cgatttcctc gtcgaggaag cgcgcgaatt cgcgcgctcc ggcgaagctc accgtcatgc
     2101 cgggcttctc gaacgcggca atcagctcgc ggtcggagag gatcctgcgc agctcggttt
     2161 ccaggcgcgc gacgacggct gcaggcgtgc ctcccgtggt catgactccg gtccagccgc
     2221 tcacctcgaa ctcgggaaat cccgactcga ccagggtcgg cgcatcggga aacatcgggc
     2281 tgcgctcgcg cgaggtgacc gcgagcagct tcagcttgcc ggccttgatc tgcggcgcgg
     2341 agatggagag cgcatcgaac atgaaatcca cgtcgccggc gaggagcgcg gccatcgccg
     2401 cgggcgcccc gcggaaggga acgtgcaccg cgtcgaactt cccgtagtac ttcagcagct
     2461 cgcccgagag gtggatgctc gtgccgttgc cgttcgaggc gaagttcagc ttgcccgggt
     2521 tgcggcgcgc gtgctcgagg agcccgcgca cgctgccgac gggggtcttc gcggagacca
     2581 cgagcacgtt cgcgccgctg cccacgagcg cgaccggggc gaggtccttc ttcgtgtcga
     2641 aaggcagcga ccggtacagc gacacgttga tcgcgttggg cggcgccgcg atgaggagcg
     2701 tatgtccgtc gggcggcgct ttggctacga agtcggtgcc gatgttgccg cccgagccgg
     2761 tcttgttctc gacgacgacc ggctgtccca aggcttccga taggcgcggg cccagagcgc
     2821 gcgcggtgaa atcggtgatt ccgcccggag gaaacgtgac gacgagccgg accggcttcg
     2881 aaggatattg agcgagcgcg gacagcgaaa gaaggatcgc gaccaccccg gcgctgcgcg
     2941 ccacccctcc tttagaagga ggggaaaaaa tcatgccgcc tgcttcgacg cggcgcgcgg
     3001 cttcttaaat cgcggcagga cttcttttgc gaccagggtg atctggtcga ccgcttcggc
     3061 gtgcgtcatg tgtccggcgt ggcccatcat gaggaaatgg ccgaggccgc cgacgttgtc
     3121 gacgaattct gccaattgcc ggtagacgtc atccggatct ccaacgaaca tgattcccgc
     3181 cttgacgagg tcctcgatcg cgccgtggtg cgtgtcgacg gccttgccct cgcgcgtggt
     3241 ggtcgtgcgc ttgccgcgcg ccttgatcat gcgcgccgcg tccttcaccg agaaatatcc
     3301 gggcggcgtg ccgaagggct cggcgacgcg gcccgaagag cgcaggtaac cgaccatgac
     3361 ttccgcgcgg cgcatcgcct ctttgcgatc ctgcgcgatc gcgacgatgc ccaggtagga
     3421 aaagcggtcc ggcgcgggcg cgggccggcc gaggtcggcg caggccttgc ggtaat
//
LOCUS       DASPZD010000381         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1174457, whole genome shotgun sequence.
ACCESSION   DASPZD010000381 DASPZD010000000
VERSION     DASPZD010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174457"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(791..>2412)
                     /locus_tag="VEM33_07385"
     CDS             complement(791..>2412)
                     /locus_tag="VEM33_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51686.1"
                     /translation="AAKDISNQTGGAMNTHGTIAVLLVFLLLGIPVFGRAQSKDQLGK
                     VDLQNSCRPAVQESFQRAVAMLHSFRYAETEKAFREVLAQDPSCAIATWGVAAILMAN
                     PLASVGPPPQWAERAQAAIDQGRKIGARTQRERDYIEAVAAYYEDWANRPERTRQLNR
                     ARAFEALAARYPTDDEAQIFYALYLAATQSLADQIYKTYLTAAETLEKQFAKHPDHPG
                     IAHYLIHSYDAPPIATQGLPAARRYASIAPAAPHALHMPSHIFTRVGAWAESAATNER
                     AAVAAKQDRDADQQVHAMDYMTYAYLQLARDGDARRVIEETAAVTGFASHAGPYGQAA
                     IPARYAIERGDWKQAVTLEPRTSKFPYTAALTHFARALGAARSGDAVAAEKDVQELAR
                     IRDTLKAAKNEYWATEVEVSRLGAAAWTALALGKREEALGLMRSAADIEDKNEKHIVT
                     PARIVPARELLGEMLLELKRPADALKEFEASHVREPQRFRGYVGAAQAAAQSGDKAKA
                     KRYFARLVELAGQGTARPELAQARAFLAANP"
BASE COUNT          352 a          815 c          818 g          427 t
ORIGIN      
        1 cggccggagg gcgagcaacg cgagcccccg gtccgctgta ctgccgagtt cgacgggccg
       61 ccgtcatctc cgctccttcg ggcccaaaat gccacgaact cgcggaagat ccccgcgaca
      121 tcctcgcgcg gtgctctcgt aaggtagcgc gaaacaaaga acttgggtgc ttccgcgatc
      181 tgcatcgggt acggacatcg cgcgatgtct ctcgcgtctt gcggaaagtc acggcaggcc
      241 gcggctacag cgttcctggc gacgtagccc atgacccaga ccgctgatgc gtcctgaatg
      301 aacggagcaa ggtgcgtggc tgcgttggcg cgcggtgatg agtacgtccg agccaatcgc
      361 ctctgcgtcg ctacttcgcc gtgcgcgagg tggcagcgga ttccatggtc gaagagaaag
      421 ccggctacac ggaaggcgtt gagcgtaggt gcttctatga tgccagcgcc atgtagtcca
      481 cggagcaaga tggttcgcac ggcgaccccg ctgtcttcgt cgtagaagta ggcggtgtcc
      541 gctgatccag gattctcgcc gacaatgaga cagcgaagag tagtcggcag ccattccgtc
      601 gcgacccgga actgatccca cgcgtgagcg ggttcatcgc agcagggcat cacccgagat
      661 gcgtcctcct cgccgtcgaa cgccgcggtc agcagccggg gcgagcaacg cgagccccgg
      721 tcttgctgca ccgtcatgtt gggcggcttg cttcgctcac gaagcgcgta taccccagcg
      781 tggagcgctg tcacgggttc gcggccagga acgcgcgcgc ctgcgccagc tccggtcgtg
      841 ctgtgccctg gccggcgagc tccaccagcc gcgcgaagta gcgcttggcc ttcgccttgt
      901 cgccactctg cgcggccgcc tgcgcggcgc cgacgtagcc gcggaagcgc tggggctcgc
      961 gcacgtgcga cgcctcgaac tccttgagtg catccgcagg acgcttcagc tcgagcagca
     1021 tctcgccgag caactcgcgt gcgggcacga tccgcgccgg cgtcacgatg tgcttctcgt
     1081 tcttgtcctc gatgtccgcc gccgagcgca tgaggccgag cgcttcctcg cgcttgccca
     1141 gcgccagcgc cgtccaggcc gccgcgccga gccggctgac ctcgacttcc gtcgcccagt
     1201 actcgttctt cgcggccttc agggtatcgc ggatccgtgc gagctcctgc acgtccttct
     1261 ccgccgcgac ggcgtcgccg ctccgcgcgg cgccgagcgc ccgcgcgaag tgcgtgagcg
     1321 cggccgtata agggaatttg cttgtgcgcg gctccagcgt gactgcctgc ttccagtcgc
     1381 cacgctcgat cgcgtagcgc gccggaatcg ccgcctgccc gtagggcccg gcgtggctgg
     1441 cgaacccggt gacagcggcg gtctcctcta tcacccggcg cgcgtcgccg tcgcgcgcga
     1501 gttgtaggta cgcgtaggtc atatagtcca tcgcgtgcac ctgctggtcg gcatccctgt
     1561 cctgcttcgc ggccacggcg gcgcgctcgt tcgtggccgc cgactccgcc caggcgccga
     1621 cgcgggtgaa gatgtgcgac ggcatgtgca gcgcgtgcgg tgccgcgggg gcgatgctgg
     1681 cgtagcggcg cgcggcgggc aacccctgcg ttgcgatcgg cggcgcgtcg tagctgtgga
     1741 tcaggtagtg cgcgatgccc ggatgatccg gatgcttcgc gaactgcttc tccagcgtct
     1801 ccgcagccgt gagatacgtc ttgtagatct ggtcggccag cgactgcgtg gcggcgaggt
     1861 agagggcgta gaagatctgc gcctcgtcgt cggtgggata gcgcgcggcc agcgcctcga
     1921 aggcgcgcgc gcgattgagc tgacgcgtgc gctccggacg gttcgcccag tcctcgtagt
     1981 aggcggcgac ggcctcgatg tagtcgcgct cgcgctgggt cctggcgccg atcttccgac
     2041 cctggtcgat cgccgcttgc gcccgttccg cccactgcgg tggcggtccg acgctggcga
     2101 gcgggttcgc catcaggatg gcagcgacgc cccacgtcgc gatcgcgcag gacggatcct
     2161 gggcgagcac ctcacggaag gccttctcag tctcggcgta ccggaacgag tgcagcatcg
     2221 cgacggcgcg ctggaagctc tcttgcactg cggggcgaca ggaattttgg aggtcgacct
     2281 tgccaagctg atccttggac tgcgcccgcc cgaagaccgg aatgccgagc agcaggaaaa
     2341 ccagcaacac tgcgatggtc ccgtgcgtat tcatcgcgcc tcctgtctga ttcgaaatgt
     2401 cctttgctgc gt
//
LOCUS       DASPZD010000382         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1617158, whole genome shotgun sequence.
ACCESSION   DASPZD010000382 DASPZD010000000
VERSION     DASPZD010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617158"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VEM33_07390"
     CDS             complement(<1..545)
                     /locus_tag="VEM33_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYS51687.1"
                     /translation="MAPRNGTEPRSVGAVRPQTLSFTEPLRFRSGAVLDSYDLVYETY
                     GTLNAAKSNAVLACHALNAAHHVAGYYADDPDNLGWWDNMIGPGKPVDTDKFFVVGVN
                     NVGGCFGSTGPKSIDPGSGKPYGSSFPVVTVEDWVDAQARLADRLGIASFAAVMGGSL
                     GAMQALQWTISYPDRIRNAIVV"
     gene            complement(695..1150)
                     /locus_tag="VEM33_07395"
     CDS             complement(695..1150)
                     /locus_tag="VEM33_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51688.1"
                     /translation="MLREVLIVEQNNPALKRRWFESDYFDLFIWQDSGGAFVRFQLCY
                     DVELNERALVWSDTEGFYHDGVDHGDGTEGGLGQAPILLRDGKFDSGTVVPRFEREAA
                     EMPADVRDFVLAKMRVHLIALYRQKMGRKRVRRERWQQRRPRFKASPRN"
     gene            complement(1184..>2245)
                     /gene="ptsP"
                     /locus_tag="VEM33_07400"
     CDS             complement(1184..>2245)
                     /gene="ptsP"
                     /locus_tag="VEM33_07400"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HYS51689.1"
                     /translation="LVIVDGREGVLIVNPGARVLEEYRRLRSEIEAERQKLKRLRTAR
                     SATMDGVTVDLQANIELPQDVKEANEAGAAGIGLFRSEFLFLNRDDLPSEDEQFEAYR
                     EVADAMHGRPVTIRTLDLGADKSINGADRGGPNPALGLRAIRFCLAEPQMFLTQLRAI
                     LRASRHGKIRLLIPMLAHAHEVEQTLAAVRQAKETLDDHKIRYDQRIQIGGMIEVPAA
                     ALSLKYFISKLDFLSIGTNDLIQYTLAIDRTDDTVAHLYDPLHPAVLQLIAQTIRSAK
                     RARTPVAVCGEMAGDAAMTRLLLGFGLREFSMHPAQLLAIKQQVLRTRVREVEKPAAR
                     VYRAHDTGRARALLAKLNA"
BASE COUNT          357 a          735 c          738 g          415 t
ORIGIN      
        1 acgacgatgg cattgcggat gcgatccgga tagctgatcg tccattgcag cgcctgcatc
       61 gcgccgaggc tccctcccat cacggcggca aaggaagcta tgcccagccg gtcggcgagc
      121 ctcgcctgcg cgtccaccca gtcctcgacc gtgacgacgg gaaacgagga accgtagggc
      181 ttcccggatc cgggatcgat gctcttcggg ccggtcgagc cgaagcagcc gccgacgttg
      241 ttgacgccga cgacgaagaa tttgtcggtg tccaccggct tgccgggccc gatcatgttg
      301 tcccaccagc cgaggttgtc cgggtcatcg gcgtagtaac cggccacgtg gtgcgccgcg
      361 ttgagagcgt gacaggcgag cacggcgttc gatttcgccg cgttcagcgt cccgtaggtc
      421 tcgtagacga gatcgtacga atccagaacc gctccgctgc gaaagcggag cggttcggtg
      481 aagctcagcg tttgcggcct gaccgcgccc accgatcgcg gctctgtccc gtttcttggc
      541 gccatgaaat aaaaaacccg tttagcctgc ctgctaaacg ggtcgccctc gctttagcaa
      601 tatttgtttg tcgcccgccg atcggtgggc ggcgcctgca agctgatgat caaatcggcg
      661 cgaaccagga acttacctca gcgcggcggc ggcgtcaatt ccttggagac gccttgaacc
      721 ggggcctgcg ctgctgccag cgctcccgcc gcacgcgctt gcggcccatt ttctgccggt
      781 acagtgcgat caggtgaacg cgcatcttgg cgaggacaaa atcgcgcacg tcggcgggca
      841 tctcggccgc ctcgcgctcg aagcgcggca cgacggtccc cgaatcgaat tttccgtcgc
      901 gcagcaggat cggcgcctgc ccgagcccgc cttccgtgcc gtcgccgtga tccacgccgt
      961 cgtggtagaa gccctcggta tcgctccata ccagggcgcg ctcgttcagc tcgacgtcgt
     1021 agcacagctg gaatctgacg aaggccccgc ccgaatcctg ccagatgaac aggtcgaaat
     1081 agtcgctctc gaaccagcgg cgcttgagcg cggggttgtt ctgctcgacg ataaggactt
     1141 ctcgcagcat cggtctcggt gcggagggtc gcgggcggca gcttcaggcg ttgagcttcg
     1201 cgagcaaggc gcgcgccctt ccggtgtcgt gcgcgcggta gacgcgcgcg gccggcttct
     1261 cgacttcccg gacgcgtgtc cgcaggacct gctgcttgat cgcgagcagc tgcgcgggat
     1321 gcatcgagaa ctcgcgcagc ccgaagccga gcagcagacg cgtcatggcc gcatcgcccg
     1381 ccatttcccc gcacaccgcc accggggtcc tggcgcgctt cgcgctgcgg atggtctgcg
     1441 cgatgagctg cagcacggcc gggtgcagcg gatcgtacag gtgcgccacg gtgtcgtcgg
     1501 tgcgatcgat cgcgagcgtg tactggatca ggtcgttggt cccgatcgaa agaaaatcca
     1561 gtttgctgat gaaatatttc aaggacagcg ccgcggcggg cacctcgatc atcccgccga
     1621 tctgtatgcg ctggtcgtac cggatcttgt ggtcgtcgag cgtttccttc gcctgccgca
     1681 cggcggcgag tgtctgctcg acctcgtgcg catgggcgag catcggaatg agcaggcgta
     1741 tctttccgtg gcgggaggcg cgcaggatcg ctcggagctg ggtcaggaac atctgcggct
     1801 cggcgagaca gaagcgtatc gcgcgaaggc cgagcgcggg attgggcccg ccccggtcgg
     1861 ccccgttgat gctcttgtcg gccccgaggt cgagggtcct gatcgtcacc ggccggccgt
     1921 gcatcgcgtc ggcgacctcc cggtaggcct cgaactgctc gtcttccgag ggcagatcgt
     1981 cgcggttgag gaacaggaat tcgctgcgga agaggccgat tcctgcggct cccgcctcgt
     2041 tggcctcctt cacgtcctgc ggcagctcga tattggcctg aaggtcgacc gtgacgccgt
     2101 ccatcgtggc cgagcgggca gtgcgcaagc gcttcagttt ctgccgctcg gcctcgattt
     2161 cgctccgcag ccgccgatat tcctcgagca cccgcgcgcc gggattgacg atcagcacgc
     2221 cttcgcggcc gtcgacgatc acgag
//
LOCUS       DASPZD010000383         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_978301, whole genome shotgun sequence.
ACCESSION   DASPZD010000383 DASPZD010000000
VERSION     DASPZD010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978301"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VEM33_07405"
     CDS             complement(<1..684)
                     /locus_tag="VEM33_07405"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HYS51690.1"
                     /translation="MRLTYLLGIFCIALCAAALARAEPAGKVLSADGDALVLRGSQIL
                     RLSRGAAVESGDQIHTGSDGKVLVVFTDSGLVWIRANSDFVVDEYSYAQGGREAAFFS
                     LLKGGARSVTGLIGRRIRSNFRLRIPVATIGIRGTDFSVVMCQRDCRDSDGSIAAKGL
                     YGEVIQGRIAVTPLDDRLLEREFGAGEFFHLADERSSPTPLFLPPPFLRSAQDEQARF
                     ADRTGIVIAP"
     gene            929..1393
                     /locus_tag="VEM33_07410"
     CDS             929..1393
                     /locus_tag="VEM33_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS51691.1"
                     /translation="MKNTVISHAASGCATALLASGVTYVTLVGWPDAMSLQAQAQTPA
                     NPGTRQTELFKTMMNDVLGRVVTLRRTERDPGNGSGPHRHPGSHTFGYVLEGTYEVKV
                     DDGPLQRLGPGGTFYEPPGALHAVSRNGSTVNPVKYLVIQISDPTKPQTVPE"
     gene            1423..2148
                     /locus_tag="VEM33_07415"
     CDS             1423..2148
                     /locus_tag="VEM33_07415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYS51692.1"
                     /translation="MKISVVVPAFNEERLLTASLTSIRAAMEGFARLGWESELIVCDN
                     NSTDRTAEIAKSAGAQVVFEPVNQIGRARNAGAARARGDWIFFVDADSNPGVELFLEA
                     ADSLRSGRCLAGGSTVRCEGPHPGVALATGIWNAVSRINKWAAGSFIFCEAAAFRETG
                     GFSEELYAGEEIDLFRRLKRLARRDRRTIVILHRHPLRTSDRKLRLYGWRNFFWFMVK
                     TIVSRGRTLRSRQDCFAWYDGRR"
     gene            complement(2179..2442)
                     /locus_tag="VEM33_07420"
     CDS             complement(2179..2442)
                     /locus_tag="VEM33_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51693.1"
                     /translation="MQEILKYLKDRGERLDSEIATDTGISLENVRLHVSALSARGEVM
                     LCQSIRFNNGKRTEGLLCRIAGYIPPAAPGRKSKAHLKAQTQT"
     gene            complement(2594..2743)
                     /locus_tag="VEM33_07425"
     CDS             complement(2594..2743)
                     /locus_tag="VEM33_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51694.1"
                     /translation="MTHVTHSKMLETRRRRERAKKDFAGTAKRAKNLAKRNAKKARAG
                     AAKPV"
BASE COUNT          517 a          937 c          970 g          540 t
ORIGIN      
        1 gggcgcgatc acgattcccg tccggtccgc gaaacgcgcc tgctcgtcct gcgcgctgcg
       61 caggaacggg ggcggcagga aaagcggcgt ggggctcgag cgctcgtccg cgaggtggaa
      121 gaattcgccc gcgccgaatt cgcgctcgag caagcggtcg tcgaggggtg tcaccgcgat
      181 cctgccctgg atgacctcgc cgtagagtcc cttggccgcg atgctgccgt cggaatccct
      241 gcagtcgcgc tggcacatga cgacgctgaa atccgtgccg cggattccga tcgtcgcgac
      301 cggaatacgg agcctgaaat tgctgcgtat gcggcggccg atcaggccgg tgacggagcg
      361 cgcgccgccc ttcaacaggc tgaagaaggc cgcctcgcgg ccgccttgcg cgtaggagta
      421 ctcgtcgacg acgaaatccg aattggcgcg tatccagacc agacccgagt ccgtgaagac
      481 gacgagcacc ttgccgtcgc tgccggtatg gatctgatcg cccgactcga cggccgcgcc
      541 gcgggagagc cgcaggatct ggctgccgcg gagcacgagc gcgtcgccgt ccgcgctcag
      601 cacttttccg gcgggttctg cgcgcgcgag cgcagcggcg caaagggcga tgcagaatat
      661 tcccagaagg tacgtcaagc gcatgcgcgc acttcccgaa gtcgtcatat ttccctcgcc
      721 atcaaagata gaaacatcac ttcgtcgttc gctgtcgccg cttttttgta ccgaatagtg
      781 tgcccgcgcg gaaagtcagc atcaaattat agcgaaggac aatgtgatat tttccctgat
      841 cagaaaggag cacgctgcgt cggaattcca tgacttcctg tagcggttgc acgacatcgg
      901 atatcatttt cgcggcaagg aggagaccat gaaaaacacc gtcatttccc atgcagcgtc
      961 tggctgcgca acggcattgc tcgcttccgg cgtcacctat gtgacgctcg tcggttggcc
     1021 cgacgcgatg tcgctgcagg cgcaggcgca gacgccggcc aatcccggaa cccgacaaac
     1081 ggagctcttc aagacaatga tgaacgacgt gctgggtcgc gtggtgaccc tgcgtcgaac
     1141 cgagcgtgat cccggaaacg gcagcggccc gcaccgtcac cccggcagcc acacgttcgg
     1201 ctacgtactg gaaggaacct acgaagtgaa agtcgacgac ggcccgctgc agaggctcgg
     1261 gccgggcggg actttctacg aaccgcccgg cgcgctgcac gccgtttcgc gcaacggcag
     1321 cacggtgaac ccggtcaagt atctggtcat ccagatttcc gatccgacca aaccgcagac
     1381 cgtgcccgaa tgaggagatg aataaaaaac ggcgcccaag aaatgaagat ctccgtcgtc
     1441 gttcccgcgt tcaacgaaga gcgattgctt accgcatcgc tcaccagcat ccgcgcagcg
     1501 atggaaggat tcgcgcggct cggctgggag tccgagctga tcgtgtgcga caacaattcg
     1561 accgaccgca cggcggagat cgcgaagagc gcgggggcgc aggtcgtgtt cgagccggtg
     1621 aaccagatcg gccgggcgcg caatgcgggc gccgcccgcg cgcgcggaga ctggatcttc
     1681 ttcgtggacg ccgactccaa tcccggcgtg gagcttttcc tggaagcggc ggattcgctc
     1741 aggtccgggc gctgtctcgc cggcgggagc accgtccgct gcgagggccc gcatcccggc
     1801 gttgcgctcg cgaccggaat ctggaacgcg gtgagccgga tcaacaagtg ggcggcgggg
     1861 tcgttcatct tctgcgaggc ggcggcgttc cgcgagacgg gcggcttcag cgaggagctg
     1921 tacgcgggcg aagagatcga cctgttccgg cggctgaagc gcctggcccg ccgcgaccgg
     1981 cgaaccatcg tgattcttca ccgccatccg cttcgcacca gcgaccgcaa gctgcggctc
     2041 tacggctggc gcaatttctt ctggttcatg gtgaagacca tcgtgtcgcg cgggcgcacg
     2101 ctgcgaagcc ggcaggactg cttcgcctgg tacgacggcc ggcgctgacg ggctcgggac
     2161 gggcgccccg ggctcttgct aggtctgcgt ctgcgccttc aggtgcgcct tggatttcct
     2221 gccgggagcg gccggcggaa tatagcccgc gatcctgcag agcaggcctt cggtcctctt
     2281 gccgttgttg aagcggatgg actggcacag catgacctcg ccgcgcgcgg agagggcgga
     2341 cacgtgcagc cgcacgtttt cgagcgagat tccggtatcc gtcgcgatct ccgagtccag
     2401 ccgctcgccg cggtctttca ggtatttcag gatctcctgc aaggcgctac tccttgtttt
     2461 ctatgccggg gatgtgcctg gccaccccga aagcggaccg acattctacg ctaaagcggg
     2521 cgatcttgcc cgggtttcca gctttccccg actaggcctt gatcgggcag gggggctaag
     2581 gcgtatcgct cgactagacg ggcttcgcgg cgcccgcgcg cgccttcttc gcgttgcgct
     2641 tcgcgagatt cttcgcgcgc ttcgccgtcc cggcaaaatc tttcttcgct ctttcccttc
     2701 tgcgccgggt ctccagcatc ttggagtggg tcacgtgggt caaactggcc tccgatcggt
     2761 tgcgcctttg aatggcgcga cagtttgacg ggtttcggtg cgcttgggta gcgcgccgtg
     2821 ctccatcagg gcagctggga gctcgagaac aggcgcagct tgggcgcggg cgcggaaaaa
     2881 taataccagg tatcgtcgat agctatctgg cccgcgggcc cttgcacaag gcccttcggg
     2941 ctgtactggt ccgaggtcgc gtac
//
LOCUS       DASPZD010000384         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1004776, whole genome shotgun sequence.
ACCESSION   DASPZD010000384 DASPZD010000000
VERSION     DASPZD010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004776"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VEM33_07430"
     CDS             <1..509
                     /locus_tag="VEM33_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51695.1"
                     /translation="VHDDTARMVQRLDDQAQEVDKHVIELRGMSASLNPSLVYAAEEY
                     LLTVRQILRNQAASHRYRIQVSASEQALRDHMRTANRRSGNWIKDAVRAKDRMEKEYF
                     DYRISVDAFGRLLESYSATRKKLAQQVGAGLLVEEAVAANARKRVLATSKRVADDVER
                     ARQLADVR"
     gene            532..1290
                     /locus_tag="VEM33_07435"
     CDS             532..1290
                     /locus_tag="VEM33_07435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYS51696.1"
                     /translation="MDSSGKVAIVTGAGSGIGRSVALAFLKDGYRVALAGRRKDKLDE
                     TAKESGAGERALVVQTDVTDAKSVKALFAATKEKFGRLDALFNNAGIGTPGSIMLEDL
                     TLEQWQAVVNTNLTGMFLCTQEAFRIMKGQTPRGGRIINNGSISAHAPRPNSAPYTST
                     KHAVTGLTKSTALDGRKYDIACGQIDIGNARTPLASRMADGVPQANGEIRPEPLMDVD
                     HVGAAVLHMASLPLDTNVQFMTIMATKMPFIGRG"
     gene            1302..2009
                     /locus_tag="VEM33_07440"
     CDS             1302..2009
                     /locus_tag="VEM33_07440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYS51697.1"
                     /translation="MNSTDPRGITLSPGLVSTSSDEVERVLSALMARREPLAALLARS
                     ADSRWKLRSVDPMRQYIVVEPLSGGSAIPALLERKQVTFVAEFGGMYIEFPASDPALV
                     TEEGTRAIRMGFPKVTVSRQQRAHPRAPVPLDLPVHCAIPAGAGVALEGQIMDISEGG
                     LGLLVCGAYLVPAPGTLIRGWQIERPGNGSVSVDLEVRHCRPLALADGASAQRWGCQF
                     VNPSSEVKELMRLFASE"
     gene            2033..>2539
                     /locus_tag="VEM33_07445"
     CDS             2033..>2539
                     /locus_tag="VEM33_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYS51698.1"
                     /translation="MSEKTIRREDARLLTGGGRYTADWNLPGQLHAFFLRSDRAHAEI
                     VSIDAEAATARKGVHVVLSGADYAASGWKSLPGGVSYEGVGGQKQKKPFWPALAQKKV
                     HYVGQPVVLVVAESAALAQDAAEEIAAEYRDLPAAVGFEGATRPGAPQLHEDIPGNLA
                     FEYETGDAA"
BASE COUNT          464 a          844 c          856 g          375 t
ORIGIN      
        1 ccgtgcatga cgatacggcc cggatggtac agaggctcga cgaccaggcg caggaagtgg
       61 acaagcacgt tatcgagctg cgcggcatga gcgcttcgct caacccatcg ctggtgtacg
      121 ccgcggagga gtacctgctc acggtaaggc agatcctgcg caaccaggcg gcgagccacc
      181 gctatcgaat ccaggtttcg gcgagcgagc aggccctgcg cgatcacatg cgcacggcga
      241 accggcgctc gggaaactgg atcaaggacg cggtgcgcgc caaggatcgc atggagaaag
      301 agtatttcga ttaccggatc tcggtcgatg ccttcggccg gctgctcgag tcctattccg
      361 caacccggaa gaagcttgcg cagcaggtcg gcgcgggcct gctcgtcgag gaagccgtcg
      421 cggccaacgc ccgcaagcgc gtgctcgcca cctcgaagcg tgtcgccgac gacgtggaac
      481 gggcgaggca gctcgccgac gtacgctgaa gctcaacagg gaaggacgaa tatggattcc
      541 tccggaaaag tggccatcgt cacgggcgcg ggcagcggca tcgggcgctc ggtcgccctc
      601 gccttcctca aggacggcta tcgcgtcgcg ctcgccggga ggcgcaagga caagctcgac
      661 gagaccgcga aggagtccgg cgcgggcgag cgcgcgctcg tcgtgcaaac cgatgtcacc
      721 gacgcaaaat cggtgaaggc cttgtttgct gcgacgaagg aaaagttcgg gcgcctcgac
      781 gcgctcttca acaacgccgg gatcgggacc cccggcagca tcatgctcga ggacctgacg
      841 ctcgagcagt ggcaggccgt ggtgaacacc aatctcaccg ggatgttcct gtgcacgcag
      901 gaggcgttca ggatcatgaa gggccagacc ccgcgcggcg ggcgcatcat caacaacggc
      961 tcgatctcgg cgcacgcgcc gcggccgaac tcggcaccct acacctccac caagcacgcg
     1021 gtcacgggcc tcaccaagtc gaccgcgctc gacggcagga agtacgacat cgcctgcggc
     1081 cagatcgaca tcggcaacgc gcgaactccg ctcgcctcgc gaatggccga cggcgtgccg
     1141 caggcgaacg gcgagatcag gccggagccg ctgatggacg tggaccacgt cggggcggcg
     1201 gtgctgcaca tggcgagcct gccgctcgat accaacgtgc agttcatgac catcatggcg
     1261 accaagatgc cgttcatagg tcgcggttga aacgatccgc aatgaactcc accgacccgc
     1321 gcggaatcac tctttccccg ggcctcgtgt ccacctcgag cgacgaggtc gagcgcgtgc
     1381 tgagcgcgct gatggcgcga cgcgagccgc tcgcggcgct cctcgcccgc agcgccgact
     1441 cgcggtggaa gctgcgctcg gtcgacccga tgaggcagta catcgtcgtc gagcccttat
     1501 cgggcgggag tgctatcccg gcgctgctcg agcgcaaaca ggtgaccttc gtcgccgagt
     1561 tcggcgggat gtacatcgag ttccccgcct cggatccggc gctcgtcacc gaggaaggga
     1621 cgcgcgcgat ccgcatgggc ttccccaagg tcaccgtgag ccgccagcag cgtgcgcatc
     1681 cgcgcgcgcc ggtgccgctg gatctgcccg tgcactgcgc gattcccgcc ggcgcagggg
     1741 tggcgctgga aggacagatc atggacatca gcgaaggcgg cctggggctc ctcgtttgcg
     1801 gcgcctatct cgtcccggcg ccgggcacgc tcatccgtgg ctggcaaatc gagcgtccgg
     1861 gaaacggcag cgtgagcgtg gatctcgaag tgcggcattg ccggcccctg gcgctcgccg
     1921 acggcgcgag cgcgcagcgc tggggctgcc agttcgtcaa tccctccagt gaagtgaaag
     1981 agctgatgcg gctgtttgcg agcgagtaaa gcgcgcttcg cgcgttcttc ccatgagtga
     2041 aaagaccata cgacgcgaag acgcgcgcct gctgaccgga ggcggccgtt acaccgccga
     2101 ctggaacctt cccggacagc ttcacgcctt ttttctccgc tccgatcgcg cgcacgccga
     2161 gatcgtctcg atcgacgccg aggccgcgac cgcgcgcaaa ggcgtccacg tcgttctcag
     2221 cggcgccgat tacgcggctt cgggatggaa atcgcttccc ggcggcgtga gttatgaagg
     2281 cgtgggaggc cagaagcaga aaaaaccctt ctggcccgcg ctcgcgcaga agaaggtgca
     2341 ctacgtcggc cagcccgtcg tcctcgtggt cgcggaaagc gcggcgctcg cgcaggacgc
     2401 ggccgaggaa atcgcggccg agtatcgcga tcttcccgcc gcggtcggtt tcgagggggc
     2461 gacgcgcccg ggcgcaccgc agctgcacga ggatatcccc ggcaacctcg ccttcgaata
     2521 cgagacgggc gacgccgcc
//
LOCUS       DASPZD010000385         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1187286, whole genome shotgun sequence.
ACCESSION   DASPZD010000385 DASPZD010000000
VERSION     DASPZD010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187286"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VEM33_07450"
     CDS             <1..578
                     /locus_tag="VEM33_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HYS51699.1"
                     /translation="GEDFRFGAKRVGDLALLKELGAFHGYEVETMPTVMHAGSRVSSS
                     AVRAALAAGDLVAAETLLARTYSISGRVVHGRKLGRELGFATANVQLKHNRPPLTGIY
                     AAKVHGIGAVARPAVASLGVRPTITASGRAVLEVHLFDFSGNLYEAHVRVEFLHKIRD
                     EEKYPDLETLKTQIARDCEAAKSFLMDHRNA"
     gene            571..>2288
                     /locus_tag="VEM33_07455"
     CDS             571..>2288
                     /locus_tag="VEM33_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYS51700.1"
                     /translation="MPENKTDYKKTLNLPDTPFPMRGDLAKREPHRVEEWQEKRIYER
                     IREASRGRPKFVLHDGPPYANGDIHIGQAVNKILKDVVVKSRNMAGFDAVYVPGWDCH
                     GMPIEVQIEKTYGKNIPVEKTLSLARAYAAEQIERQKKDFQRLGVLGDWNNAYTTMAY
                     KTEADEIRTLGKILEKGYVYRGLKPVNWCFDCGSALAEAEVEYIDRIDLAVDVGFPFA
                     EPGKIADAFGLKKLPTENGWIVVWTTTPWTIPANQALNVHPDFSYDLVETERGLLILA
                     ADLVETCLARYGLKGRTLASCKGGKLELIGFRHPFYDRIAPVYLGEYVTLEQGTGIVH
                     SAPAYGVDDFVSCRRYGMKDEQILTPVMGDGKYASSLPFFGGLDIWTANSRIVEKIRE
                     AGALFHSEKHPHSYMHCWRHRTPIIYRATTQWFAGMDEVPGYNGVKPAETLRAAALRG
                     VEATRFYPSWGQARLHGMIANRPDWTLSRQRQWGVPMPFFIHKETGALHPRTLELLEA
                     VAKKVELGGIEAWQRIDAKDLLGADAERYEKVKDTLDVWFDSGSTHQTVLKGSHAKEL
                     KFPADL"
BASE COUNT          463 a          715 c          732 g          378 t
ORIGIN      
        1 tcggcgagga ctttcgcttc ggagcgaagc gcgtcggcga tctcgctttg ctgaaagaat
       61 tgggcgcgtt tcacgggtat gaggtcgaaa ccatgccgac ggtgatgcac gccggttcgc
      121 gggtatccag ttcggcggta cgcgccgccc tcgccgcggg cgacctcgtc gcggccgaaa
      181 cgctgctcgc gcggacctac agcatcagcg ggcgcgtggt ccacggacga aagctcgggc
      241 gcgagctggg attcgccacg gccaatgtgc aactcaagca caatcgccct ccgctcaccg
      301 gcatctacgc ggcgaaggtg cacggcatcg gggcggtcgc ccgccccgcg gtcgcgagcc
      361 tcggcgtgcg gcccacgatc acggcgagcg ggcgcgcggt actggaggtg cacttgttcg
      421 atttttcagg gaatctctac gaagcgcacg tgcgcgtcga gttcctgcac aagatccgcg
      481 acgaggagaa atatcccgat ctcgagacct tgaagaccca gattgcgcgc gactgtgaag
      541 ccgccaagag cttcctcatg gatcaccgca atgcctgaga acaagaccga ctacaagaaa
      601 acgctgaacc ttccggacac gccctttccc atgcgcggcg acctggcgaa gcgcgagccg
      661 caccgggtcg aggagtggca ggagaagaga atttacgaaa ggatccgcga ggcaagccgc
      721 ggtcggccga agttcgtgct gcacgacggg ccgccgtacg cgaacggcga catccacatc
      781 ggccaagccg tcaacaagat cctcaaggac gtcgtcgtga agtcgcgcaa catggcgggc
      841 ttcgacgcgg tctacgttcc cgggtgggac tgccacggca tgccgatcga ggtgcagatc
      901 gagaagactt acggcaagaa cattcccgtg gaaaagactc tgagcctcgc gcgcgcgtat
      961 gcggccgagc agatcgagcg acaaaaaaag gacttccagc gcctgggcgt cctgggcgac
     1021 tggaacaacg cctacacgac gatggcgtac aagaccgaag cggacgagat caggacgctc
     1081 ggcaagatcc tcgagaaggg ctacgtctat cgcgggctga aacccgtgaa ctggtgtttc
     1141 gactgcggct ccgcgctcgc cgaggccgaa gtcgagtata tagaccgaat cgatctcgcc
     1201 gtcgacgtcg ggtttccttt tgccgaaccg gggaaaatag cggatgcctt cgggttgaaa
     1261 aagctgccta cggagaacgg ctggatcgtc gtctggacga cgactccctg gacgattccc
     1321 gcgaaccagg cgctcaacgt ccatccggat ttttcctacg acctcgtcga gaccgaacgc
     1381 ggcctgctga tcctcgccgc cgacctggtg gagacctgtc tcgcgcgcta cgggctgaaa
     1441 ggcaggacgc tcgcctcctg caagggcggg aagctcgagc tgatcggatt ccgccatccc
     1501 ttctacgacc gcatcgcacc cgtttacctc ggcgagtacg tgacgctgga gcagggtacg
     1561 ggcatcgtcc acagcgctcc ggcttacggc gtcgatgact tcgtttcctg ccgccgctac
     1621 ggcatgaagg acgagcagat tctcaccccg gtgatgggcg acggcaaata cgcttcgtcg
     1681 ctgccgtttt tcggcggact ggacatctgg acggcgaatt ccaggatcgt cgagaagatc
     1741 cgcgaagcgg gcgcgctgtt tcattcggaa aagcacccgc acagctacat gcactgctgg
     1801 cgccacagga ccccgatcat ctaccgcgcc accacccagt ggttcgcggg catggacgag
     1861 gtgcccggat acaatggcgt gaaacccgcg gaaaccctgc gggccgccgc gctgcgaggc
     1921 gtggaggcga cgcgcttcta cccttcctgg ggccaggcgc gcctgcacgg catgatcgcc
     1981 aatcgccccg actggacgct gtcgcgtcag cgccagtggg gcgttccgat gcctttcttc
     2041 atccacaagg agaccggcgc cctgcacccg cgaaccctgg aactgctgga agccgtcgcg
     2101 aaaaaggtgg agctgggagg tatcgaagcc tggcagcgga tcgatgcgaa ggatctgctc
     2161 ggtgccgacg ccgaacggta cgaaaaggtg aaggacacgc tcgacgtctg gttcgattct
     2221 ggctcgacgc accagaccgt gctgaaaggc tcgcatgcga aggagttgaa gtttcccgcg
     2281 gacctcta
//
LOCUS       DASPZD010000386         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1788404, whole genome shotgun sequence.
ACCESSION   DASPZD010000386 DASPZD010000000
VERSION     DASPZD010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788404"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VEM33_07460"
     CDS             <1..723
                     /locus_tag="VEM33_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYS51701.1"
                     /translation="DDIESVRQAASRDKSVVAVLVEPIQGEGGINVSSMQYLRDLREL
                     CDRQGWLLMLDEVQCGLGRTGKWFVYQHAGIQPDVVLLAKGLASGVPIGACLARGAAA
                     AVFKPGTHGSTFGGNPLACAAALATLAAIEEAGLMKNAERVGGVLLAGLREALAGCPG
                     VAEVRGHGLMIGVELDRPCGDLVGQALEAGLLINVTMDKVIRLLPPLVMNEGEARHVL
                     SILVPLVRSFLAQPAPAMPARA"
     gene            720..1673
                     /gene="argF"
                     /locus_tag="VEM33_07465"
     CDS             720..1673
                     /gene="argF"
                     /locus_tag="VEM33_07465"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYS51702.1"
                     /translation="MKKGQARLRHYLQFKDFSRREYEHLFARTRWIKDKFKRYERYWP
                     LEDRTLAMIFEKPSTRTRLSFEAGMHQLGGAAIYLYTRDSQLGRGEPVEDAGQVISRM
                     CDLVMIRTFQQEILSRFAASSRVPVINGLTNEYHPCQVLADIYTYVEHRGSIRGKTVA
                     WIGDSNNVCNTWLQAATIFDFNVHVSTPPGYEVEPERAGIYHDANYESFADPMDAARG
                     ADLVTTDVWTSMGFESEKEERGRDFENWQVDADMMREAKKGALFMHCLPAHRGEEVAG
                     EVIDGPQSVVWEEAENRLHTQKALMEFLLLGKVKKGRRGKK"
     gene            1670..2896
                     /locus_tag="VEM33_07470"
     CDS             1670..2896
                     /locus_tag="VEM33_07470"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HYS51703.1"
                     /translation="MKGKVKKVVLAYSGGLDTSVVLKWLQDTYGCEVVTFTADIGQGE
                     ELEPARKKARKLGVKEIFVDDLREEFARDFVFPMFRANTIYEGEYLLGTSIARPLIAK
                     RQIEIARKTGADAVAHGATGKGNDQVRFELGYYALEPNIRVIAPWREWNLTSREKLLE
                     YAEKHGIPIEGRKKSGSPYSMDANLLHISYEGRILEDPSREPEEEMWRWTVSPEKAPQ
                     RGEVAELQYERGDIVAVNGKKLSPARVLAELNRLGGKHGVGRLDLVENRYVGMKSRGC
                     YETPGGTIMLKAHRAIESICLDREVAHLKDDLMPRYASLIYNGYWWSPERVALQTLID
                     HTQQHVNGSVRVKLYKGNVDVVGRESRTDSLFDTTIATFEDDRGAYDQADAAGFIRLN
                     ALRMRIAANRRQGKRR"
     gene            complement(2903..>3016)
                     /locus_tag="VEM33_07475"
     CDS             complement(2903..>3016)
                     /locus_tag="VEM33_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYS51704.1"
                     /translation="AGDVIACGSSVGVGAMTPGSTVSIEIEGIGTLTNKFE"
BASE COUNT          564 a          970 c         1016 g          466 t
ORIGIN      
        1 gacgacatcg aatcggtgcg ccaggccgcg tcccgcgaca agagcgtcgt cgcggtgctg
       61 gtcgagccga tccagggcga aggcggaatc aatgtctcct cgatgcagta cttgcgcgat
      121 ctgcgcgagc tctgcgaccg ccagggatgg ttgctcatgc tggacgaagt ccagtgcggt
      181 ctgggccgca cgggcaagtg gttcgtctat cagcacgccg ggatccagcc cgacgtcgtg
      241 ctgctcgcca agggcctcgc ctcgggcgtt cccatcggag cctgcctcgc acgcggcgcc
      301 gcggcagcgg tgttcaagcc gggaactcac gggtccacgt tcggcggcaa tccgctcgcc
      361 tgcgcggcgg ccctcgctac gctcgccgcc atcgaggaag caggtctcat gaagaacgcc
      421 gagcgcgtcg gcggagttct cctcgccggg ctccgcgagg cgctcgcggg atgtccgggc
      481 gtggccgagg tacgcggtca cggccttatg atcggcgtcg agctcgaccg gccctgtgga
      541 gatctggtcg ggcaggctct ggaggcgggc ctgctcatca acgtcaccat ggacaaagtg
      601 atccggctgc tgccgccgct cgtgatgaac gaaggcgaag cccgccatgt gctttccatc
      661 ctcgttccgc tggtgcgctc gttcctcgcg cagcccgccc ccgcgatgcc cgcgcgcgca
      721 tgaaaaaagg gcaggcccgg ctgcggcact acctgcagtt caaggacttc tcgcgccggg
      781 agtacgagca cctgttcgcg cgcacgcgct ggatcaagga caagttcaag cgctacgagc
      841 gctactggcc tctcgaagac cgtaccctcg ccatgatctt cgagaagccc tcgacgcgca
      901 cgcgcctctc gttcgaggcg ggcatgcacc agctcggcgg cgcggcgatc tacctgtaca
      961 cgcgggattc gcagcttggg cggggggagc cggtcgagga cgccgggcag gtgatctcgc
     1021 ggatgtgcga tctggtgatg atccgcacct tccagcagga gatactgtcg cgctttgccg
     1081 cgagctcgcg cgtcccggtg atcaacggcc tcaccaacga ataccacccc tgccaggtgc
     1141 tcgccgacat ctacacctac gtcgagcacc gcgggtcgat ccggggcaag acggtggcct
     1201 ggatcggaga ctcgaacaac gtgtgcaaca cctggcttca ggcggcgacg atcttcgatt
     1261 tcaacgtgca cgtctcgacc ccgccgggct acgaggtgga gcccgagcgc gccgggatct
     1321 accacgacgc gaactacgag agcttcgccg acccgatgga cgccgcgcgc ggcgcggacc
     1381 tcgtcaccac cgacgtgtgg accagcatgg gcttcgagtc ggagaaggag gagcgcgggc
     1441 gcgatttcga gaactggcag gtggacgcgg acatgatgcg cgaggcgaag aagggcgcgc
     1501 tgttcatgca ctgcctgccc gcgcaccgcg gcgaggaagt cgccggcgaa gtgatcgacg
     1561 ggccgcagag cgtggtctgg gaggaggcgg agaaccgact gcacacgcag aaagcgctga
     1621 tggaatttct gctgctcggc aaggtcaaga aagggagaag ggggaaaaag tgaagggcaa
     1681 ggtgaagaag gtcgtcctcg cctactcggg cgggctcgat acctcggtcg tcctcaaatg
     1741 gctgcaggac acctatggct gcgaggtcgt caccttcacc gccgacatcg gacaggggga
     1801 ggagctcgag cctgcgcgga aaaaggcgcg gaagctgggc gtgaaggaga tattcgtgga
     1861 cgacctgcgc gaggagttcg cgcgcgactt cgtgtttccc atgttccggg cgaacacgat
     1921 ctacgagggt gaataccttc tgggaacctc gatcgcccgc ccgcttatcg ccaagcgcca
     1981 gatcgagatc gcgcgcaaga ccggggcgga cgcggttgcg cacggcgcga ccggcaaggg
     2041 caacgaccag gtacgcttcg agctcggcta ctacgcgctc gagccgaata tccgcgtgat
     2101 cgccccctgg cgcgagtgga acctgacttc gcgcgaaaaa ctgctcgaat acgcggagaa
     2161 gcacggcatc ccgatcgaag gcaggaaaaa aagcgggtct ccctactcga tggacgcgaa
     2221 tctgctgcac atttcgtacg agggccgcat cctcgaggac ccctcgcgcg agcccgagga
     2281 ggagatgtgg cgctggaccg tgtcgcccga aaaggctccc cagcgcggcg aggtcgccga
     2341 actgcaatac gagaggggcg atatcgtcgc agtgaacggc aagaagctca gccccgcaag
     2401 agtgctcgcg gagctgaatc gcctgggcgg aaagcacggc gtcgggcggc tggacctggt
     2461 cgagaaccgc tacgtcggca tgaaatcgcg cggctgctac gagacgccgg gcggcacgat
     2521 catgctgaaa gcgcaccgtg cgatcgagtc gatctgcctg gaccgcgagg tcgcccatct
     2581 caaggacgat ctcatgccgc gttacgcctc gctcatctac aacggctact ggtggagccc
     2641 ggagcgcgtc gcgctgcaga cgctcatcga ccacacgcag cagcacgtca acggcagcgt
     2701 gcgcgtcaag ctctacaagg gcaacgtcga tgtcgttggt cgcgagtcga ggaccgactc
     2761 gctgttcgac acgacgatcg ccacgttcga ggacgaccgc ggcgcctacg accaggccga
     2821 cgccgccggc ttcatccgcc tgaacgcact gcgcatgcgc atcgccgcga accggcggca
     2881 agggaaaaga cgctgagcgt tcctattcga atttgttggt gagcgtgccg atgccctcga
     2941 tctcgatgct caccgtgctg ccgggcgtca tcgccccgac gcccaccgag ctgccgcagg
     3001 cgatcacgtc gcccgc
//
LOCUS       DASPZD010000387         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_37037, whole genome shotgun sequence.
ACCESSION   DASPZD010000387 DASPZD010000000
VERSION     DASPZD010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37037"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1389)
                     /locus_tag="VEM33_07480"
     CDS             complement(82..1389)
                     /locus_tag="VEM33_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYS51705.1"
                     /translation="MNVKRGQRAVLAALLALLAADAAAALTAAEQQIVAAVKERSGAA
                     LQFLERTVNVNSGTLNPEGVREVGRMFRAEFDQLGFTTRWVDLPPAVQRAGHLVAARE
                     GKQGKRLLLIGHLDTVFEKDSPVQRWERKGDRIRGPGVSDMKGGDVIIVEALRALAKL
                     HALDNTSITVVFTGDEEKPGSPLEVTRAELVSAAKRSDLALGFEGIVKDREGRDTATI
                     GRRASSGWMLTVTGKPAHSSGVFSESSGFGAIYEGARILNAFREQVAEPDLTFNPGAV
                     VGGTEVSYDPAVNKGSAFGKTNVIAAGFRAQGDLRYLTYEQRDRAHARMRAIVAKSLP
                     GAKAEITFSESYPPMSPTPGNLRLLEGYSQASVDAGLGVIEALPPGQRGAGDVQFVAP
                     YVACLDGIGPSGEGSHTNDEDLEPGSIERATIRAALLIYRLTR"
     gene            complement(1464..2546)
                     /gene="serC"
                     /locus_tag="VEM33_07485"
     CDS             complement(1464..2546)
                     /gene="serC"
                     /locus_tag="VEM33_07485"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HYS51706.1"
                     /translation="MTRVFNFSAGPAVLPEEVLEKAASEMLDWHGSGMSVMEMSHRGK
                     EFIAIAEQAEKDFRELLKVPKNYKVLFLQGGATLQFGMVPMNLLGGKKSADYIYTGEW
                     SKKAIKEANRFCEINIAASSESKNFSFAPEQSTWQLDPKSAYVHYTSNETIGGVEFHW
                     IPNTGKVPLVADASSHILSRPLDVSKFGLIYAGAQKNIGPAGLTLVIVREDLLGQAVP
                     HTPSMLDYKVHADSGSMSNTPATYAWYIAGLVFQWLKAQGGLAAMERKNIAKAKLLYD
                     FLDSSAFFKNPVRKSDRSRMNIPFTLADSKLDGEFLKGAEARGMMQLKGHRAVGGMRA
                     SIYNAMPVEGVKRLVEYMKEFEAKRG"
BASE COUNT          450 a          880 c          849 g          521 t
ORIGIN      
        1 tatttcgcga acatgccctt cggcgttatg actgcggcgc gctggagaat cgattatcgt
       61 atccgtcgac cgccacgctc atcagcgcgt caggcgatag atgagcaagg ccgcgcgaat
      121 cgtcgcgcgc tcgatcgacc cgggctccag gtcctcgtcg ttggtgtgcg acccctcgcc
      181 gctcgggcca atgccgtcga gacacgcgac gtaaggagcg acgaactgga cgtcgcccgc
      241 gccacgctgt cccggtggca gcgcttcgat gacaccgagc cccgcatcca cgctggcttg
      301 cgaataccct tccagcaggc gcaggttccc gggcgtgggc gacatcggcg gatacgactc
      361 gctgaacgtg atttccgctt tcgcgccggg caggctcttc gccacgatcg cgcgcatgcg
      421 ggcgtgtgcc cggtcgcgct gctcgtaagt gagatagcgc aggtcgccct gggcgcggaa
      481 acccgccgca atcacattgg tcttgccgaa ggcgctgcct ttgttgacgg ccgggtcgta
      541 ggacacctcc gtgccgccca ccacggcgcc gggattgaag gtgagatccg gttcggccac
      601 ttgctcgcga aaggcgttca gaatgcgcgc gccttcatag atcgcaccga agcccgaaga
      661 ttcgctgaac acgccagaag aatgtgccgg cttgccggtg acggtgagca tccagccgga
      721 ggaggcgcgc cggccgatgg tcgcggtatc cctgccctct cggtccttca cgatgccttc
      781 gaagcccagc gcgagatcgc ttctcttggc cgcgctgacg agctcggcgc gcgtgacctc
      841 caaaggcgaa cccggcttct cttcgtcacc ggtgaacacg acggtgatgc tcgtgttgtc
      901 cagcgcgtgc aatttggcga gtgcccgcag cgcttcgacg atgatcacat cgccgccttt
      961 catgtcggat acgcccgggc cgcgaatgcg atcgcccttg cgctcccaac gctggacggg
     1021 actgtccttt tcgaacacgg tgtcgaggtg gccgatcaaa agaagccgct tgccctgctt
     1081 gccttcacgc gccgccacga gatggcccgc ccgctgcacg gcgggcggca agtcgaccca
     1141 gcgggtggtg aagccgagct ggtcgaattc ggcgcggaac atgcgcccca cctcgcgcac
     1201 gccttcggga ttcaaggtgc cgctgttgac gttcaccgtg cgctcgagaa actgcaaggc
     1261 ggcaccggag cgttccttca ccgcagcgac gatctgctgc tcggcggcag tgagcgcggc
     1321 tgctgcgtcg gccgcaagca atgccagaag ggcggcgagc accgctcgct gcccgcgctt
     1381 cacattcatg tctcctcctg gtttttctaa agtaaggaac ttggtgatga gaatagccgc
     1441 agccgctgcg gcgcttgaag cggttacccc cgcttagcct cgaactcctt catgtactcg
     1501 accagccgct tcacgccctc gaccggcatg gcgttgtaga tcgaggcgcg catgccgccg
     1561 acggcacggt ggcctttcaa ctgcatcatg ccgcgggctt cggcgccttt cagaaactcg
     1621 ccgtcgagct tggagtccgc gagcgtgaac gggatgttca tgcgcgagcg gtcggacttc
     1681 cgcaccgggt tcttgaagaa cgcgctcgag tcgaggaaat cgtagaggag tttcgccttg
     1741 gcgatgtttt tcctttccat cgccgcgagg ccgccttgcg ccttcagcca ctggaacacg
     1801 aggccggcga tgtaccaggc gtaggtcgcc ggggtgttcg acatcgagcc gctgtcggcg
     1861 tgcaccttgt agtcgagcat cgacggcgtg tgcggaacgg cttgcccgag caggtcttcg
     1921 cgcacgatga cgagggtcaa tccggccggt ccgatgttct tctgcgctcc cgcgtagatc
     1981 aggccgaact tggagacatc gagcggccgc gacaggatat gcgaggacgc gtccgcgacg
     2041 agcggcacct tgcccgtatt cgggatccag tggaactcca ccccgcctat ggtctcgttc
     2101 gaggtgtagt gcacgtaggc ggacttcggg tcgagctgcc acgtcgactg ctcgggcgcg
     2161 aagctgaagt tcttgctctc ggagctcgcc gcgatattga tctcgcagaa gcggttcgcc
     2221 tccttgatcg ccttcttcga ccactcgccc gtatagatat agtcggccga cttctttccc
     2281 ccgaggaggt tcatcggcac catgccgaac tgcagcgtcg ccccgccctg gaggaagagc
     2341 accttgtagt tcttggggac cttgagcagc tcgcggaagt ccttctccgc ttgctccgcg
     2401 atcgcgatga actccttgcc gcggtggctc atctccatca ccgacatgcc cgatccgtgc
     2461 cagtcgagca tctcggaggc ggccttctcg agcacctcct ccggcaggac cgctgggccg
     2521 gcgctgaagt tgaagacgcg ggtcacgggc agccgaactc gacgatcgtc ataagaacgt
     2581 ccagcgcacg ccgagcgaaa agctgctgct gttgtattcg acggtgtgcc cctcgatctt
     2641 ggcgtcctgg gcgctctggt atcggtattc aacgaacagg cgcaacggtc cgttgaacag
//
LOCUS       DASPZD010000388         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1083672, whole genome shotgun sequence.
ACCESSION   DASPZD010000388 DASPZD010000000
VERSION     DASPZD010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083672"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VEM33_07490"
     CDS             complement(<1..298)
                     /locus_tag="VEM33_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51707.1"
                     /translation="MNRHFAAIFAALAVFAASAAHAQQPVKIGLIMPYSGQFADAATQ
                     MDNAIKLYMKEHGETVAGRKLEIIRRDTGGVAPDVAKRHAQELVVRDRVDILAGF"
     gene            complement(399..929)
                     /locus_tag="VEM33_07495"
     CDS             complement(399..929)
                     /locus_tag="VEM33_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HYS51708.1"
                     /translation="MAARKKESAARAPFKLVSPDIRPNGPIAMEQVFNGFGCSGENVS
                     PALGWSGAPEATKSFALLVHDPDAPTGGAGWWHWLVINIPASATELRKDAGKADGSNL
                     PSGCAHIDTDFGGAGWGGPCPPAGDKAHRYNFTLYALKVDGLDASGASASLAGYMINA
                     NAIGKAKLTGKFGRKK"
     gene            1038..2093
                     /locus_tag="VEM33_07500"
     CDS             1038..2093
                     /locus_tag="VEM33_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS51709.1"
                     /translation="MPKMTRAALFPLVAACSLVPGALRAQPADVAALALYGGSDREQR
                     LVKGARAQREINLYSSLVLEDLNALAAAFDKKYGVKLKFWRAGSEKVVQRIVTEARAG
                     RFDFDVVETNGPELESLHREMLLQKAGSPHFPDLMPEAIRPHREWVGTRLNMFVHAYN
                     TNLVRKEELPKSYEDFLDPKWKGRLGIEAEDVDWFAMVIKEIGEDKGLTLFRAIVARN
                     GLSVRRGHTLLAGLVASGEVPFALTVYNHNAEKLKQRGAPVDWYAIQPAIARVNGVAL
                     SRKPAHPHAAVLFYDFMLGEGQEILVQGNYVATNRKADPGAARASRLRFVDPAAMLDE
                     SAKWEKLYAEIITRQSK"
     gene            2117..>2318
                     /locus_tag="VEM33_07505"
     CDS             2117..>2318
                     /locus_tag="VEM33_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HYS51710.1"
                     /translation="MLVLERIVSVIGPVFLIVAIGYGYGRRNRPDLKSFNHVSLHVLG
                     PLLVFTSLAGEDFQLEGNAAFVA"
BASE COUNT          386 a          756 c          760 g          416 t
ORIGIN      
        1 cgaacccggc caggatgtcg actcggtcgc gcaccaccag ctcctgcgcg tggcgcttgg
       61 caacgtccgg cgcgacgccg cccgtgtccc tgcggatgat ctcgagcttc ctgcctgcga
      121 ccgtttcgcc gtgctccttc atgtagagct tgatggcgtt gtccatctgc gtcgctgcgt
      181 ctgcgaactg gccggaatag ggcatgatca gcccgatctt gacgggctgt tgcgcatgcg
      241 ccgcagacgc ggcgaaaacc gcgagcgccg cgaagatcgc ggcgaaatgt cgattcatgg
      301 tgtgtcctcc tcggcagcta ccatagcaag gcgggcacgg cgccgcaagt tgggacgaat
      361 cccacggtgc gtccgggctc acccggccca ccgatcttct actttttccg tccaaatttc
      421 ccggtgagct tcgccttgcc gatcgcgttg gcattgatca tgtatccggc gagggaggcg
      481 ctcgcgccgc tcgcgtcgag cccgtccacc ttcagcgcgt acagcgtgaa gttgtagcgg
      541 tgcgctttgt cgccagccgg cgggcagggg ccgccccagc ccgcgccgcc gaaatcggtg
      601 tcgatgtgcg cgcagccgga aggcaggttg gaaccgtcgg ccttgcccgc gtcctttctc
      661 agctcggtcg cgctcgcagg aatgttgatg acgagccagt gccaccagcc cgctccgcct
      721 gtcggggcat ccgggtcgtg caccagcagc gcgaagctct tcgtggcctc aggcgccccc
      781 gaccagccga gcgcgggcga gacgttctcg cccgagcagc cgaagccgtt gaacacctgc
      841 tccatggcga tcgggccgtt cggccggatg tcggggctta cgagcttgaa cggagcgcgc
      901 gcggcgctct ctttttttct tgcagccatg gtacctccca gtgagatggc ggagcgacga
      961 ttgtatccaa gcggcgcccc gggcgcattt ataatgccgc gaagcttaca ttttgatgaa
     1021 ttcggcgcgg aggatgaatg cctaagatga cgcgcgctgc gctcttcccg ctcgtcgcgg
     1081 cttgctcgct cgtgcccggg gccttgcgcg cacagcccgc cgacgtcgcg gcgctggcgc
     1141 tgtacggtgg aagcgatcgc gaacagcgcc tcgtcaaagg cgcgcgcgca cagcgcgaga
     1201 tcaacctcta ctcgtcgctc gtgctcgagg atctcaatgc gctcgccgcg gcgttcgaca
     1261 agaagtacgg ggtgaagctg aagttctggc gcgccggctc cgaaaaggtg gtgcagcgca
     1321 tagtcaccga ggcgcgggcc ggccgcttcg acttcgacgt cgtcgagacc aacggtcccg
     1381 agctcgagtc gcttcaccgc gagatgctgc tgcagaaggc gggatcgccg cacttcccgg
     1441 acctgatgcc cgaggcgatc aggccgcacc gcgaatgggt cgggacgcgc ctcaacatgt
     1501 tcgtccacgc ctacaacacg aacctggtga ggaaggagga gctgcccaag tcctacgagg
     1561 actttctcga ccctaagtgg aaagggcgcc tggggatcga ggccgaggac gtcgactggt
     1621 tcgcgatggt gatcaaggaa atcggcgagg acaagggcct gacgctgttc cgcgccatcg
     1681 tcgcgcggaa cgggctctcg gtgcgccgcg ggcatacgct gctcgccggc ttggtcgcct
     1741 cgggcgaggt ccccttcgcg ctcaccgtct acaaccacaa cgcggagaag ctgaagcaga
     1801 ggggcgcgcc cgtggattgg tatgcgattc agccggcgat cgcgcgggtc aacggcgtcg
     1861 cgctctccag gaaaccggcg cacccgcatg cggcagtgct gttctacgat ttcatgctcg
     1921 gcgagggaca ggagatcctg gtccagggga attacgtggc gaccaaccgc aaggcggatc
     1981 ccggcgccgc aagggcctcg cgcctgagat tcgtcgatcc tgcggcgatg ctggacgaga
     2041 gcgcgaagtg ggaaaaactc tacgccgaga tcataacgcg gcagtcgaaa tgagcgcgaa
     2101 gagctgacga ccgcccgtgc tcgtcctcga acgcatcgtt tcggtcatag ggcccgtctt
     2161 cctgatcgtc gcgatcggat acggctacgg gcgccgcaac cggcccgacc tgaagtcgtt
     2221 caatcacgtg tcgctccacg tgctcggccc cctactcgtt ttcacttctc ttgcggggga
     2281 ggacttccag ctcgaaggta acgccgcttt cgtcgccg
//
LOCUS       DASPZD010000389         3832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1258005, whole genome shotgun sequence.
ACCESSION   DASPZD010000389 DASPZD010000000
VERSION     DASPZD010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258005"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..629)
                     /locus_tag="VEM33_07510"
     CDS             complement(282..629)
                     /locus_tag="VEM33_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51711.1"
                     /translation="MTSRTETAPDSAKLESMREEIRLHLEDLRRALAAEILSYPTPIP
                     RCDAQFNHLYEQQARLARDLDRIGAFAEKNPGREDSIELIGRFIASAPYTDDAGERAL
                     RSRLKTELSTAGK"
     gene            complement(638..1612)
                     /locus_tag="VEM33_07515"
     CDS             complement(638..1612)
                     /locus_tag="VEM33_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51712.1"
                     /translation="MKVRSTALALAAVLAGIPAAAAQSYPSRPVTLVVPFAAGGPVDT
                     IARIVGAPMGKTLGQTVVVESVVGAAGTLGVGRVARAAPDGYTLSIGHWGTHVVNGAV
                     YPLSYDLLNDLEPVAMIASNPLMIVANASLPARDLKELIAWLKANPGKASAGTAGSGS
                     ATHIGGIYFQNATGTRFELVPYKGSGPAMLDLVGGRIELMIDQASNSIPQVRGGKIKA
                     YAITAKTRLAAAPEIPTVDEAGLPGLHVSVWYGIWAPRGTPRDVVAKLNAAVVAALAD
                     AAVRARLADLGQEIPPREQQTPEALAAFHKAEIGKWWPMVKAAGIRPE"
     gene            1694..1918
                     /locus_tag="VEM33_07520"
     CDS             1694..1918
                     /locus_tag="VEM33_07520"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HYS51713.1"
                     /translation="MARIVKRNRDHPYEVKIGGEAQYICACGLSGNLPFCDGSHKLTK
                     GETPGKLHWYHESGKRHDAKDAYPEIRSDE"
     gene            complement(1964..2203)
                     /locus_tag="VEM33_07525"
     CDS             complement(1964..2203)
                     /locus_tag="VEM33_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51714.1"
                     /translation="MDKRKAWREQEQQLVERWNAAASRLRDAQAALSSPSSENGDAAP
                     HEERVLKAKAARAELEAVRKQVARLKVEFSAGKRY"
     gene            complement(2342..2548)
                     /locus_tag="VEM33_07530"
     CDS             complement(2342..2548)
                     /locus_tag="VEM33_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYS51715.1"
                     /translation="MNTGTVKWFNDSKGFGFITPDGGGKDLFAHFSAIQGSGFKSLKE
                     NQKVSFDVTAGPKGDQAVNIKPIA"
     gene            complement(2724..3794)
                     /locus_tag="VEM33_07535"
     CDS             complement(2724..3794)
                     /locus_tag="VEM33_07535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYS51716.1"
                     /translation="MEGPSPVETGDDPLAPYRGRILYGLASAAAVCLLPFSVNAFVQG
                     NPGLGAGILAAFLVLAIDAFAIYLKKSPPIPLILLLVPIAAGTAVSLKVQGFFGALWA
                     YPTVLLFTFALSRRMANVGSILLLLMISALVYHYIGLAYTIRFFVTLTLTIVLANIIL
                     SIILDLHRRLLDQALVDPLTGVFNRRHMERCLSDAIERLRRNSAPTSLLLIDVDRFKS
                     INDQYGHATGDSVLKGIVSLIQKRSRKLDLLFRIGGEEFMLLLPDTQEAAAAVVAEEL
                     RASTAESRLLEEYPVTLSIGVAELRPGESPDSWIKHADDALYAAKKAGRNRVVCAGRP
                     APLPDPALRDAEPETRSGAERA"
BASE COUNT          688 a         1231 c         1233 g          680 t
ORIGIN      
        1 ccgaggaacg aggaccgcat gaacactacg accgccttgc ccggcgcggg agcctttacc
       61 gccgttcccg ccggcatttc ttgcatgtac tttatggtgc cgcacccgga aacaaggagc
      121 gcgagcgctg caataccgag caatctggac cacaagcccg tctgctgcgt catgatcttt
      181 ccttcctttg aaaaaaatat tcgctccttt tttgccgcag gcggcgggag cttccgagca
      241 ttctagccag cacgacacgc gagtgtaagg ccagtgcaat tttattttcc ggccgtggaa
      301 agctcggtct tcagacggga ccgtagcgcg cgctccccgg cgtcgtccgt gtaaggcgca
      361 gaggcaatga atcggccgat cagctcgatg gagtcttcgc gccccggatt cttttccgcg
      421 aacgcaccga tgcggtccag atcgcgggcg agcctcgcct gctgctcgta gaggtgattg
      481 aattgcgcat cgcaccgcgg gatcggcgtc ggatagctca ggatctccgc ggcgagcgcg
      541 cgcctgagat cctcgaggtg gagcctgatt tcctcccgca tcgactcgag cttcgccgag
      601 tccggcgcgg tttccgtcct agaggtcact gaggacttca ttccggcctg atgcccgcgg
      661 ccttcaccat cggccaccac ttcccgatct cggccttgtg aaacgcggcc agggcctccg
      721 gggtctgctg ctcgcgaggc ggaatctcct ggccgagatc tgcgaggcgc gcccgcacgg
      781 ccgcatcggc gagcgcagcg acgacggctg cgttcagctt cgccacgacg tcgcggggcg
      841 tgcctcgcgg cgcccagatc ccgtaccaca ccgaaacatg cagccccggc aggcccgctt
      901 cgtccacggt gggtatttca ggcgccgccg cgaggcgtgt cttcgcggtg atcgcataag
      961 ctttgatctt gccgccgcgc acctgcggaa tggagttcga agcctggtcg atcatcagtt
     1021 cgatgcggcc gccgacaaga tccagcatag ccggccccga gcccttgtac ggcacgagct
     1081 cgaaccgggt gccggtcgcg ttctggaagt agattccgcc gatatgcgtg gctgaaccgg
     1141 acccggcggt tcccgccgag gccttgcccg gattggcttt gagccacgcg atcagttcct
     1201 tcaggtccct ggccggcaac gaagcgttcg cgacgatcat cagaggattg ctcgcgatca
     1261 tcgccaccgg ctccaggtcg ttgagcaggt cgtacgagag cgggtagacc gcgccgttga
     1321 cgacgtgcgt gccccagtgg ccgatgctga gcgtatagcc gtctggggcg gcgcgtgcga
     1381 cgcggccgac gccaagggtg ccggccgctc cgacgacgct ttccaccacg acggtctggc
     1441 cgagcgtctt ccccatggga gcgccgacga tgcgggcgat cgtgtccacc ggcccgcccg
     1501 cggcaaacgg caccaccagc gtgaccggcc gcgacgggta gctctgggct gcggccgccg
     1561 ggatgcccgc gagaaccgcg gcgagggcga gggccgtcga tcgaactttc atttccctcc
     1621 cctgcgggga acgccgagca cggagtttaa gtctattatg aacaccgtca aacgggacag
     1681 ttgaggagag ctcatggcac gaatcgtaaa gcgcaaccgc gaccatccct atgaagtcaa
     1741 gataggcggc gaggcccaat acatctgcgc ctgcgggctc tcgggcaacc tacccttctg
     1801 cgacggttcg cacaagctca ccaagggcga gacgccgggg aagcttcact ggtatcacga
     1861 aagcgggaag cgtcacgacg cgaaggacgc ctatcccgag atccgcagcg acgagtagcg
     1921 cctggggagc acggagccgc cgcaaagaga tgcttgcgcc tttctagtag cgctttccgg
     1981 cgctgaactc caccttgagg cgcgccacct gctttcgcac cgcctcgagc tcggcccggg
     2041 ccgccttggc cttcagcacc ctttcttcgt gcggcgctgc atcgccgttc tccgaagacg
     2101 gggacgacaa ggctgcctga gcgtcgcgca gccggctcgc cgccgcgttc caccgctcca
     2161 cgagttgctg ttcctgttcc cgccacgctt ttcgcttgtc catggagcgg caggataatg
     2221 gaagcgcccc cgttctgtcg aggaatgggc gttcgctgcg agcaccttcg acccgccctt
     2281 ttcggagccc ctatgaaaaa agcccggtca gccgggcttt tttctgggcc tgcaacgctt
     2341 gttacgcgat gggcttgata ttgacggcct ggtcgccttt cgggcctgcg gtcacatcga
     2401 aggacacctt ctggttctcc ttcagggact tgaagccgct gccctggatc gccgagaagt
     2461 gcgcgaacag atccttgccg ccgccgtccg gggtgatgaa cccgaaaccc ttggagtcgt
     2521 tgaaccactt gaccgtacct gtattcattt gcacatttcc taaaaaattg taagagggct
     2581 tacgcccgga acgcgggaac ttcaagggga gagaagaagc gacgacctga tgagatgagc
     2641 gtgcctaagc ggctgcagct ggaccagacg atctcgactt ggagaatcct gcgcgctcct
     2701 tatacacgcg caccccggac gcgtcaagcg cgctccgcac cggaccgggt ctcaggttcc
     2761 gcgtcgcgga gcgccgggtc cggcaggggc gctggccgcc cggcgcaaac cactcgattg
     2821 cggcccgcct tcttcgccgc gtacagggcg tcatcggcgt gctttatcca ggaatccggt
     2881 gattcgccgg gccgaagctc ggcgacgccg atgctcagcg tcaccgggta ctcttcgagc
     2941 aggcgcgact ccgcggtcga agcgcgaagc tcctcggcga cgacggcggc tgcggcttcc
     3001 tgcgtgtcgg gcagcagcag catgaactcc tcgccgccga tgcgaaagag caggtcgagc
     3061 ttgcgcgagc gtttctggat cagcgagacg atgcccttca gcacgctatc gcccgtcgcg
     3121 tggccgtatt gatcgttgat gctcttgaag cgatcgacgt cgatcaggag gagcgaagtc
     3181 ggggccgagt tgcgccgcag ccgctcgatg gcgtcgctca ggcagcgctc catatggcgg
     3241 cgattgaaca cgccggtcag cgggtccacg agggcctggt cgaggagcct gcggtgcagg
     3301 tcgagaatga tgctcaggat gatgttggcc agcacgatcg tgagggtcag cgtcacgaaa
     3361 aaccggatgg tgtacgcaag gccgatgtag tgatacacga gcgcgctgat catcagcagc
     3421 agaaggatgc tcccaacgtt ggccatgcgc cgggacaggg cgaaagtgaa gagcagcacg
     3481 gtggggtagg cccacagcgc gccgaaaaaa ccctggacct tgagggaaac cgccgtgccc
     3541 gccgcgatgg ggacgagcag caggatcagc ggaatcggcg gagatttctt caggtagatg
     3601 gcgaaagcgt cgatggcaag aacgaggaac gctgccagaa ttcccgctcc cagcccggga
     3661 ttcccctgca cgaaggcgtt gacggagaac ggaagtaggc agaccgccgc cgcgctcgcg
     3721 aggccgtaca ggatccgccc gcgatagggg gcgaggggat catctccggt ttcgactggg
     3781 cttgggcctt ccatcgcgcc tccccggggt cgcggtcacc tacgagtctc tc
//
LOCUS       DASPZD010000390         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_38243, whole genome shotgun sequence.
ACCESSION   DASPZD010000390 DASPZD010000000
VERSION     DASPZD010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38243"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VEM33_07540"
     CDS             <1..594
                     /locus_tag="VEM33_07540"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYS51717.1"
                     /translation="CALRDEDSFRCWSLGSAEESEAVLIRRFFDGIEKYTPQLVSWNG
                     GGFDLPVLHYRSLVHGVTAPRYWEQGDEDKDFRYNNYIARYHMRHVDLMDVLSLYQNR
                     GQAPLDEMARLLGFPGKLGLDGSKVWEAYQAGEIESIRNYCAIDAANTYLVFQRFQLI
                     RGQCDEEQYRKELQLVRETFAKSGEEHWREFVGRWSR"
     gene            608..1903
                     /gene="rlmD"
                     /locus_tag="VEM33_07545"
     CDS             608..1903
                     /gene="rlmD"
                     /locus_tag="VEM33_07545"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF009639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HYS51718.1"
                     /translation="MPFARIESLDREGRGVAHVDGKTIFVDGAITGEDVEYSAYRKKP
                     AWEAAQIVRIEQEGAARVSPRCAFFGTCGGCSMQHIEERAQVAAKQRVLEDALWHLGR
                     VRPETVLRPPHGLAWGYRNRARMSVRWVEKKGGSLVGFRERRSSYVTEMTSCEVLPPK
                     VSALIRPLRTLIDTLDMQGRIPQIEVAVGDALTALVLRVLEAVEVEDRERLKRFAEAH
                     GVQLWLQPEGPDSTRPFWPPAAPGLYYTLPEYGLRIAFGPTDFTQVNHAVNRLLVGRA
                     VSLLDPRPGERIADFFCGLGNFTLPIASRGAQVLGVEGSAGLLARAQENARANGLAER
                     CRFVLADLFDARACAGLPPCDKALLDPPREGAIELVKSFAAKPPSRIVYVACDPATLA
                     RDSGVLVHTQGYRLAAAGVVNMFPHTSHVESIALFERDQ"
     gene            complement(1946..2590)
                     /locus_tag="VEM33_07550"
     CDS             complement(1946..2590)
                     /locus_tag="VEM33_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HYS51719.1"
                     /translation="MQQHRVLRNTYWLLALSLIPTVIGAAVGTNLDFGFMRTSPILSF
                     FAVLAIFYGWIFAIERNRNSSLGVGLLLGFTLFMGLLLGPLLQSVLGLRNGIQLVMMA
                     FGGTAAVFAVMAGIATTTKRNLSGMANFLAVGAVVIMLAVVANVFFQSMTAYLVILAG
                     FVLLSSLLMLWQINAIVRGGETNYVSATLTLYVSIYNLFSSLLQLLGIFGGSRD"
BASE COUNT          494 a          856 c          899 g          434 t
ORIGIN      
        1 tgcgcgctgc gcgacgagga ttcgttccgc tgctggtccc tgggcagcgc cgaagagagc
       61 gaggccgtgc tcatccggcg ctttttcgac ggcatagaga aatacacccc ccagctcgta
      121 tcgtggaacg gcggcggctt cgaccttccg gtgctgcact accgcagcct cgttcacggt
      181 gtgaccgcgc cgcgctactg ggagcagggt gacgaggaca aggacttcag gtacaacaac
      241 tacatcgccc gttaccatat gcggcacgtg gacctgatgg acgtcctgtc gctctaccag
      301 aaccggggcc aggctccgct ggacgaaatg gcgcgcctgc tgggtttccc cggcaagctc
      361 ggcctggacg gctcgaaggt ttgggaagcc taccaggccg gcgagatcga gagcatccgc
      421 aactactgcg cgatagacgc cgcgaacacg tacctcgtgt tccagcgctt tcagctgatc
      481 cgcggccagt gcgacgaaga gcaataccgc aaggagctgc agctggtgcg tgaaacgttc
      541 gccaaatcgg gcgaagagca ctggcgcgag ttcgtcgggc gctggagccg gtagctcagg
      601 cagcggcatg ccgttcgccc gcatcgaatc cctggaccgg gaagggcgcg gcgtcgccca
      661 cgtcgacggc aagacgatat tcgtcgacgg cgcgatcacc ggcgaagacg tcgagtactc
      721 cgcttaccgc aagaagcccg cgtgggaagc cgcgcagatc gtgcgcatcg agcaggaggg
      781 cgcggcgcga gtttccccgc ggtgcgcttt cttcgggacc tgcggcggat gctcgatgca
      841 gcacatcgaa gaacgggcgc aggtggcggc caagcagcgc gtgctcgaag acgcgctctg
      901 gcacctcggt cgggtccgtc ccgagacggt gctgcggccg ccgcacgggc tcgcgtgggg
      961 ataccgcaac cgcgcgcgca tgtcggtgcg ctgggtggag aagaagggcg ggtccctggt
     1021 cggatttcgc gaacgcaggt cgagctacgt caccgagatg acgagctgcg aggtgctgcc
     1081 gccgaaagtg tcggccctga tccggccgct gcgcacgctc atcgacaccc tcgacatgca
     1141 ggggcgcatt ccacagatcg aagtcgccgt cggcgatgcg ctgacggcgc tcgtgctgcg
     1201 cgtgctcgaa gccgtcgaag tggaggaccg cgaacgcctc aagcgcttcg ccgaggccca
     1261 cggggtgcag ctgtggctgc agcccgaagg gccggactct acgcgcccgt tctggcctcc
     1321 ggcggcgccg ggcctgtact acacgctgcc cgaatacggg cttcgcatcg cgttcggccc
     1381 gaccgatttc acccaggtca accacgcggt gaaccggctg ctcgtgggcc gggccgtgtc
     1441 gctcctcgac ccgcggccgg gcgagagaat cgccgatttt ttctgcgggc tgggcaattt
     1501 cacgctgccg atcgcaagcc gcggcgccca ggtgctcggg gtggaaggca gcgcaggcct
     1561 gcttgcccgc gcgcaagaga acgcccgggc caacggcctc gcagagcgct gccggtttgt
     1621 gcttgcagat cttttcgacg cgcgagcgtg cgccggactg cccccgtgcg acaaggcgct
     1681 tctcgatccg cccagggaag gggcgatcga gctggtgaaa tcattcgccg cgaagccgcc
     1741 gtcccggatt gtgtacgtcg cctgcgatcc ggcgactctc gcccgcgaca gcggcgtgct
     1801 cgttcacacg caggggtacc ggctcgccgc ggccggcgtg gtcaacatgt tcccgcacac
     1861 ctcgcacgtg gagtcgatcg cgctgttcga gcgcgatcaa taaaaaaggg cgctggagcg
     1921 ccccttttcg gtccggatcg gactcttagt cgcggctgcc gccgaagatg ccgagcagtt
     1981 gcagcaagct cgagaacagg ttgtagatcg agacgtacag cgtcagagtg gccgagacat
     2041 agttcgtctc gccgccgcgc acgatcgcgt tgatctgcca cagcatcaga agcgacgaga
     2101 gcagcacgaa gcccgccagg atcacgagat acgcggtcat actctggaag aagacgttgg
     2161 cgaccaccgc gagcatgatg acgactgcgc ccaccgcgag gaaattcgcc atgccgctca
     2221 agttgcgctt ggtcgtggtc gcgattccgg ccatcacggc gaacaccgcg gcggtgccgc
     2281 cgaaggccat catcacgagc tggatgccgt tcctcaatcc aagcacggac tggagcaatg
     2341 gtcccagcag caggcccatg aagagagtga atccgaggag caggccgacg ccgagcgagc
     2401 tgttgcggtt gcgctcgatc gcgaaaatcc atccgtagaa gatcgccagc accgcgaaaa
     2461 acgacaggat cgggctcgtg cgcatgaacc cgaagtcgag attcgttccg accgcggcgc
     2521 cgatgaccgt gggaatgagc gacagtgcga gaagccaata ggtgttgcgc agcacccggt
     2581 gctgctgcac cgcatagtct cgcgccggta acggactacc ggtccgaatc ggttgaagtt
     2641 caggttgcat tttcagcctc cgtgaaaacg ttgatgcgtc ctt
//
LOCUS       DASPZD010000391         9759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_519167, whole genome shotgun sequence.
ACCESSION   DASPZD010000391 DASPZD010000000
VERSION     DASPZD010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519167"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /gene="rapZ"
                     /locus_tag="VEM33_07555"
     CDS             complement(<1..651)
                     /gene="rapZ"
                     /locus_tag="VEM33_07555"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HYS51720.1"
                     /translation="MQLVLISGLSGSGKSIALNALEDSGYYCVDNLPGPLLQQSVELL
                     RRAGHNHVAMSIDARMGDSLELVPEYVAALKAQGVDLRLLFLDAKNDTLIRRFSETRR
                     RHPLATGMRTLEEALTREREVLAPVGEIGYRLDTTDLLPNTLRNWIKEWLDIRAETTI
                     LLFESFSYKQGVPLDADLVFDVRCLPNPHYDPRLRPLTGLDADVVAFLEREPKVGTM"
     gene            complement(717..1640)
                     /gene="hprK"
                     /locus_tag="VEM33_07560"
     CDS             complement(717..1640)
                     /gene="hprK"
                     /locus_tag="VEM33_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HYS51721.1"
                     /translation="MLQISVAQLYDDNRDKLGFAWIGGKAGGATKLWRDSTGVAALVG
                     HLNLIHPNRIQVLGNHEIAHLAAFEEADLFQTLGNLFAVHPVAIVVADGAPADVRMLK
                     AAEASGTAVFATDMPGAQVIDRLRRYLGKALAETTQCHGVCMDVLGLGVLITGDSGAG
                     KSELALELISRGHGLVADDVVDVSRIAVTTLECRCPPLLKDFLEVRGLGVLNIRTIFG
                     ETAVRPKMNLRLIVHLDRPGTNESERLPLHEQAEDILGVTVRKVVIPVAAGRNLAVLL
                     EAAVRNYILQLRGIHSTAEFMQRQQAEMKKD"
     gene            complement(1609..2094)
                     /gene="ptsN"
                     /locus_tag="VEM33_07565"
     CDS             complement(1609..2094)
                     /gene="ptsN"
                     /locus_tag="VEM33_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HYS51722.1"
                     /translation="MNLVAKLLPPSNILLDLDVTSKKRMFEQAGLLFENNQGVARSLV
                     FDSLFARERLGSTGLGQGLAIPHGRIKGLKEAVGAFVRLAQPVPFDAPDGKPVSLVFL
                     LLVPEQATEQHLRVLSELAQMLSDRELREKLSTDTDAGTLHQHITAWQPHVADQRRAA
                     V"
     gene            complement(2247..2573)
                     /gene="raiA"
                     /locus_tag="VEM33_07570"
     CDS             complement(2247..2573)
                     /gene="raiA"
                     /locus_tag="VEM33_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYS51723.1"
                     /translation="MNLTISGRHLAVTSSIRSYIESKLSRIRRHFDHVIDVNVILALD
                     HLARKAEVSCHVAGKEIFVKSGDPDLYAAIDAVADKLDRQIMKYKDRLHAHPHDALKH
                     HSPSES"
     gene            complement(2592..4034)
                     /locus_tag="VEM33_07575"
     CDS             complement(2592..4034)
                     /locus_tag="VEM33_07575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HYS51724.1"
                     /translation="MKQTLQLKLSQHLTLTPQLQQSIRLLQLSTMELNQELEKFLAEN
                     PLLEREDTDSEIPAPPAHMNGEQATAIQAPAEEASTENPSQADIFSADPDWGGEGGSS
                     SGARDDADDGDFFQVPAESPNLREHLHRQLSLTQLEPRDRNLVAFLIEALDEGGYLTQ
                     SIEELAQLVPEEVQIEAEELAIALKHLQNFDPTGVGARNCAECLELQLLALPESTPSR
                     ATALAIAHEHLELLAARDFSKLRKALQCGDDELRQAQRLIQSLNPRPGAAFSTTETRY
                     IVPDVVVKKIKNVWVASLNPDALPKLRINRMYADILQNNRGQSGGQLSHQLQEARWLI
                     KNVQQRFETILRVSQAIVDRQRHFFEHGEVGMRPLVLREIANTLGLHESTVSRVTTQK
                     FMFTPRGIFELKYFFGSHVATETGGACSATAIRALLKQLVAAEDARKPLSDSKISEIL
                     SQQGIVVARRTIAKYRESMQIPSVSMRKSL"
     gene            complement(4123..4845)
                     /gene="lptB"
                     /locus_tag="VEM33_07580"
     CDS             complement(4123..4845)
                     /gene="lptB"
                     /locus_tag="VEM33_07580"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HYS51725.1"
                     /translation="MSQLRVHGLKKHYGSRVVVKDVSLEVASGEVVGLLGPNGAGKTT
                     CFYMIVGLIAVDDGAIHLDGREISHFAMHRRARMGLSYLPQEASVFRKLTVEENVRAV
                     LELQELPEEEIESRLSGLLRDLNIAHLRQSTALSLSGGERRRVEIARALATRPTFILL
                     DEPFAGVDPIAVLDIQKIIRFLKERGIGILITDHNVRETLGICDHAYIINDGLVLAFG
                     KPDEIVYNENVRRVYLGENFRL"
     gene            complement(4877..5395)
                     /gene="lptA"
                     /locus_tag="VEM33_07585"
     CDS             complement(4877..5395)
                     /gene="lptA"
                     /locus_tag="VEM33_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HYS51726.1"
                     /translation="MTSESALAEKADHDKPITIESDSMISDGTKKVSTFEGKVVLVQG
                     SLIIRADRIVVRQDGDGFYYGVASGKPATFRHKREGYGEYIDGEAFRIEYDNKLERVE
                     FYNAARLRRDSGDDIRGDFISYDARTERFTVKSTGEASGTEREGRVRATIMPKKPAEP
                     ETPPDSGTSRKD"
     gene            complement(5439..6017)
                     /gene="lptC"
                     /locus_tag="VEM33_07590"
     CDS             complement(5439..6017)
                     /gene="lptC"
                     /locus_tag="VEM33_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HYS51727.1"
                     /translation="MKTRVTPLLPLVLMAFLGLLTLWLQYAVVEGSSGDAKSARHDPD
                     SIVENFTVQRLDATGKLRYTFSAPKMMHFADDGSGEVLNPRFVEIAADGGNFIATANR
                     GTINRQGEEAFLYGNVLFLREATPARSEFRARTEFLHVLAEQGIARTDHTVSIAEGSS
                     MLTGVGMIVNEKNQQFMLQSQVKGIFDVPSRK"
     gene            complement(6014..6541)
                     /locus_tag="VEM33_07595"
     CDS             complement(6014..6541)
                     /locus_tag="VEM33_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit delta"
                     /protein_id="HYS51728.1"
                     /translation="MRDDVLERMRRLRLMIFDVDGVLTDGTLYFSETGAELKAFNAQD
                     GHGLKMLKESGVEVAILSARRSRAVEVRAAELGITLVEQGAGNKGAAFENLIGRLRTS
                     AAAAGYMGDDWADLPVLARCGFAASVPEAPQLVRERVHHVTRAPGGRGAVREVCELIM
                     RAQDTLERLIARQLA"
     gene            complement(6541..7533)
                     /locus_tag="VEM33_07600"
     CDS             complement(6541..7533)
                     /locus_tag="VEM33_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HYS51729.1"
                     /translation="MNDRSKKAISTSALELARKVLTIEADAVSGLISRLDERFVDAVG
                     LILACRGRVVVSGVGKSGHIARKIASTLASTGTPAFFVHAAEAGHGDLGMITRDDVVL
                     ALSNSGQSDELLTIIPRIKRQGAKLIALTGNPDSALAREADVHLDAGVAQEACPLNLA
                     PTASTTAALALGDALAVALLDARGFDEQDFARAHPGGALGRKLLTRVSEVMRTGKDIP
                     AVSDTDSFSTMLAEISGKGMGMTAILGHGKKLVGIFTDGDLRRTLESKPDIRGLKITD
                     VMKRDPRTIAPHKLAAEAVELMDRHRISQLLVVAETGELAGALSLHDLFRAKVI"
     gene            7649..9619
                     /locus_tag="VEM33_07605"
     CDS             7649..9619
                     /locus_tag="VEM33_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HYS51730.1"
                     /translation="MGDTLELVLILLASAVFVVVLFRRLHLPPLLGYLLVGVAVGPFA
                     LAWIPDTAEGRHLAEFGVVFLMFSIGLEFSLPKLFQLRRAVFGLGLAQVLLTLGAVLA
                     VAIFAGAPWQAALAVGGAIAMSSTAMVLRILAERIQLETPHGREIVGVLLFQDLAVVP
                     LLILIPAIAEGSGELAQRLGFALVKAAVVLVVLLFVGQRLMRGWFHIVARRHSHELFI
                     LNVLLITLGLAWLTDRAGLSLALGAFLAGMLIAETEYRHQVEEDIRPFREVLLGLFFV
                     TMGMRLDPGTVAANFSLTLALTALLVVLKFILVAGLARLFGAGPGAAVRTGLALAQVG
                     EFGLVLMVLAQSVGLLDRDFSQVVVAAMLLSMLAAPFLIQASDKLALRWSSAEWMLQS
                     LALHRVAAQSLATERHVLVCGYGRTGQRLAHMLEQEHIDVIALDTDPERVRGAEAAGE
                     KVVFGDASRYETLVAAGVARASALVITFADTQLALRILHHVRALNPALPVIVRTLDDA
                     DMDRLIAAGASEVVPETFESSLMLASHALVLLGVPLRRVVRRIREVREHRYSLMRGFF
                     HGGTDEAEDPDEAREPRLHSVTLATGASASGRSLGEIALDKLGVRVTALRRRELRMIS
                     PDPQTALEEGDVIVLLGTPEQLAAAEMRLLEG"
BASE COUNT         1613 a         3012 c         3205 g         1929 t
ORIGIN      
        1 catcgtgccg accttgggct ccctctcgag aaacgcgacc acgtccgcgt cgagaccggt
       61 gagcggccgc agccgcgggt cgtaatgcgg attgggcagg cagcgcacgt cgaataccag
      121 gtccgcgtcg agcggaacgc cttgcttgta gctgaaggac tcgaacagga gaatcgtcgt
      181 ttccgcgcgg atgtcgagcc attccttgat ccagttgcgc agcgtattcg gcagcaaatc
      241 ggtcgtgtcg aggcgatagc cgatttcgcc cacgggagcg agaacctcgc gctcgcgtgt
      301 cagggcctcc tcgagcgtgc gcatcccggt cgcgagggga tggcgcctgc gtgtttcgga
      361 gaagcgccgg atcagcgtat cgttttttgc atcgaggaag agcaggcgca gatccacgcc
      421 ctgagctttc agcgctgcca cgtattccgg gacgagctcg agcgaatcgc ccatgcgcgc
      481 gtcgatgctc atcgcaacat gattgtggcc cgcgcggcgc agcaattcca ccgattgctg
      541 gagcagcggc cccgggagat tgtcgacgca gtagtaaccg ctgtcctcca gggcattgag
      601 ggcgatgctc ttcccggaac ccgacagccc gctgatcagg acgagttgca tttcgacacc
      661 ttagcggaat ggcggaagcc gcgcaaaacc tggcccggcc agctcactcg tccgcttcaa
      721 tctttcttca tctccgcctg ctgcctctgc atgaattcgg cggtcgagtg gatgccgcga
      781 agctgcagaa tgtagttgcg cacggcggcc tcgagcagca ctgcgaggtt gcggcctgcc
      841 gcaaccggaa tgacgacttt ccggacggtg acgcccagga tgtcttcggc ctgttcgtgc
      901 agcggaagtc gctcagactc gttcgttccc gggcgatcga gatgcacgat cagccgcaag
      961 ttcatcttcg ggcgcaccgc agtctcgccg aatatcgtgc ggatgttgag cacgcccagg
     1021 ccgcgcactt ccagaaaatc cttcaatagc ggcgggcaac ggcattcgag cgtcgttacc
     1081 gcgatgcgcg agacgtcgac gacgtcgtcc gcaacgagcc cgtggccgcg gctgatcagt
     1141 tccagcgcga gctcgctttt cccggcgccc gagtcaccgg tgatgagcac tccgagcccg
     1201 agcacgtcca tgcatactcc gtggcactgc gtggtctccg cgagcgcctt gccgagatag
     1261 cggcgcagcc ggtcgatgac ttgcgcaccg ggcatatcgg tggcgaacac cgccgtgccc
     1321 gaggcctcgg ccgccttcag catgcgcaca tcggcgggcg ccccgtcggc gacgacgatc
     1381 gctaccgggt ggaccgcgaa cagattgccg agcgtctgga agagatcggc ctcctcgaat
     1441 gcggcgagat gggcgatctc gtggttgccg aggacctgga tgcggttggg atggatgagg
     1501 ttgaggtgcc ctacgagcgc tgcaacgccg gtgctgtcgc gccaaagctt ggtcgcgccg
     1561 cccgcctttc cgccgatcca ggcgaacccg agcttgtcgc gattgtcgtc atacagctgc
     1621 gcgacgctga tctgcaacat gaggttgcca ggccgtgatg tgctggtgaa gcgttcccgc
     1681 atcggtgtcg gtcgagagtt tctcacgcag ctcgcggtcg ctcagcatct gtgcgagctc
     1741 ggacaggacg cgcaggtgct gctcggtcgc ctgttccggc accaacagca ggaacaccag
     1801 actgacgggc ttgccgtcgg gcgcgtcgaa cggaaccggc tgcgccaggc gcacgaaagc
     1861 cccgaccgct tccttcagac ccttgatcct gccgtgcgga atggcgagtc cctgccccag
     1921 gccggtcgag ccgagacgct cgcgggcgaa caggctgtcg aacaccaggc tcctggccac
     1981 cccctggttg ttctcgaaaa gcaacccggc ctgctcgaac atgcgtttct tgctcgtcac
     2041 atccaggtcc aggaggatgt tcgaaggggg cagcagcttg gcaactaggt tcatctgtga
     2101 gattcgtcca gtccaagcct tgcgtaggca gaaatcgtgc ccggcggccc ggaaaggcgt
     2161 ggaattctac accatcggct cagggggctg ttgagcgcgc ttccccctga aaatgacgca
     2221 gggagaggca gtccgcgatg aggagtctaa gattccgagg gggaatggtg cttgagcgca
     2281 tcgtgcggat gcgcatgcag acggtccttg tacttcatga tctggcgatc gagtttgtcc
     2341 gctacggcgt cgatcgcggc gtacagatcc gggtcgccgc tcttgacgaa gatttccttt
     2401 ccggcgacgt gacagctgac ttccgccttt cgtgccagat ggtccaaggc gaggatcacg
     2461 ttgacgtcga tgacgtgatc aaagtgccgc cggatcctgg agagcttgct ctcgatgtaa
     2521 ctgcggatcg aagatgtgac cgccaagtga cgcccgctga tcgtgagatt catgcgaagc
     2581 ggaccctcgc gtcagaggga ctttctcatg ctcacggagg gaatctgcat cgactcccgg
     2641 tacttggcga tggtgcgacg cgcgaccacg atgccctgct ggctgaggat ctcggagatc
     2701 ttgctgtccg aaagcggctt tcgcgcgtcc tctgcggcaa ccagctgctt gagcagcgcc
     2761 cggatagcgg tcgccgaaca ggcgcctccg gtctcggtgg cgacgtggct cccgaagaag
     2821 tatttgagct cgaaaatgcc gcgcggcgtg aacatgaact tctgggtcgt gacgcgcgag
     2881 acggtcgatt cgtgcaggcc gagcgtgttt gcgatttcgc gcagcacgag cggccgcatc
     2941 ccgacctcgc cgtgctcgaa aaaatggcgc tggcggtcga cgatcgcctg cgacacgcgc
     3001 aggatcgtct cgaatcgctg ctgcacgttc ttgatcagcc agcgcgcctc ctggagttga
     3061 tgcgagagct ggccgccgga ctgaccgcga ttgttctgaa ggatatcggc atacatgcga
     3121 ttgatgcgca gcttcggcag cgcgtcgggg ttgaggctcg ccacccagac gttcttgatc
     3181 ttcttgacta cgacgtcggg tacgatatag cgcgtttcgg tggtcgagaa cgcggcgccg
     3241 ggacgcgggt tgaggctctg gatcaggcgt tgggcctggc gcagttcgtc gtctccgcat
     3301 tgcagcgcct tcctgagctt cgaaaagtcg cgggccgcga ggagctcgag gtgctcgtgc
     3361 gcgatggcga gggccgtggc gcggctcggc gtggactcgg gcagggctag cagctgcaac
     3421 tcgagacatt ccgcgcaatt gcgggcaccc acgcccgtcg ggtcgaagtt ctgcaggtgc
     3481 ttgagggcga tggccagctc ctccgcctcg atctgcacct cctcgggcac gagctgcgcg
     3541 agctcctcga tcgactgcgt gagatagccg ccttcgtcca gcgcttctat gaggaacgcg
     3601 accaggttgc ggtcgcgcgg ttcgagctgg gtgagggaga gttgccggtg cagatgctca
     3661 cgcaggttgg gagactcggc ggggacttgg aaaaaatcgc catcgtcggc atcgtcgcgc
     3721 gcacccgagc tcgagccgcc ttctcccccc caatcggggt cggccgagaa aatgtccgcc
     3781 tgcgacggat tctcggtgct cgcctcctcg gcgggcgcct gaatggcggt cgcctgctcg
     3841 ccgttcatgt gcgcaggggg tgccggaatc tcgctgtcgg tgtcttcgcg ctcgaggagc
     3901 ggattttccg cgagaaactt ctcgagttcc tgattcaatt ccatcgtcga gagctgcagc
     3961 aggcgtatcg attgctgcag ctgcggcgtc agcgtcaggt gctgcgagag cttgagctgt
     4021 agagtctgtt tcatcgaaat gggacgcggg cacctcgcca ggaggatcgt agttcaggcg
     4081 ggccaaattg ggaaaccggg gggtggttcg cggcgttgtc ccctacagcc tgaaattctc
     4141 gccgagataa acgcgtctaa cgttttcatt ataaacgatc tcgtcgggtt tgccgaaggc
     4201 gagcaccaag ccgtcgttga tgatgtaggc gtggtcgcaa atccccagcg tttcccgcac
     4261 gttatggtcc gtgatgagaa tgccgatgcc gcgttccttc agaaagcgga tgattttctg
     4321 aatgtcgagc accgcgatcg ggtcgacgcc cgcgaagggt tcatcgagca gtatgaaggt
     4381 cggacgcgtc gcgagcgccc gggcgatctc gactcggcgg cgctcgccgc ccgacaggga
     4441 cagagcggta gattgccgca gatgcgcgat gttgagatcg cgcaggagcc cggaaaggcg
     4501 ggactcgatc tcctcctcgg gaagttcctg cagttccagc accgcgcgga cgttctcttc
     4561 gacggtcagc ttgcggaaca cggaggcttc ctggggcagg tacgacagtc ccatgcgggc
     4621 gcggcgatgc atcgcgaagt ggctgatctc tctgccgtcg agatggatcg cgccgtcgtc
     4681 caccgcgatc agcccaacga tcatgtaaaa acaggtcgtc ttcccggcgc cgttcgggcc
     4741 cagcagcccc acgacctcac cgctcgccac ctcgagcgac acgtccttga ccacgactcg
     4801 cgagccgtag tgctttttga gtccgtggac ccgcagctgg ctcatcgagt tcgccccgtt
     4861 cggatgtacg gggaaattag tccttccggc tcgtgccgga gtctggcggg gtttcgggct
     4921 ccgccggctt tttcggcatg atggttgcgc gcacgcgtcc ttcgcgttcg gtcccggacg
     4981 cctcgccggt cgatttcacg gtaaaacgct cggtccgggc gtcgtaggaa atgaaatccc
     5041 ctcgaatgtc gtccccgctg tcgcggcgca gccgggcggc gttatagaac tcgacgcgtt
     5101 cgagcttgtt gtcgtattcg atcctgaacg cttctccgtc gatgtattct ccgtagccct
     5161 cccgcttgtg gcggaacgtc gccggcttcc cgctcgcgac gccgtagtag aagccgtcgc
     5221 cgtcctgccg cacgacgatc cggtccgcgc ggatgatgag cgagccctgg accagaacga
     5281 cttttccctc gaaagtggaa actttcttgg ttccgtcgga aatcatgctg tcggattcga
     5341 tggtgatcgg tttgtcgtga tcggctttct cggcaagcgc cgattctgat gtcaaggccg
     5401 acattgccgc aagacccagg gccgcggctg cgcgcgccct atttcctgga cggcacatca
     5461 aagatgcctt tcacttgcga ctgcagcatg aactgttgat tcttttcgtt cacgatcatc
     5521 ccgacgccgg tcaacatcga gctcccctcc gcaatcgaaa ccgtgtgatc tgtacgcgcg
     5581 atcccctgct cggcgagtac gtggaggaat tccgttcgag cgcgaaactc ggatcgcgcc
     5641 ggcgttgctt cgcgcaagaa aagcacattg ccgtacagga aagcctcctc gccttggcga
     5701 ttgatggtgc cgcgatttgc ggtcgcgatg aaattgccgc cgtcagcggc aatttcaacg
     5761 aagcgcggat taaggacttc gcccgaaccg tcgtcggcaa aatgcatcat cttcggcgcg
     5821 gagaacgtgt accgcagctt gcccgtggcg tccagccttt gaacggtgaa gttttcgacg
     5881 atggaatcgg gatcgtgcct ggcggacttt gcgtcgccgc tggaaccctc caccacggcg
     5941 tattgcagcc aaagggtcag caacccgaga aacgccatca gaacgagcgg gagcagaggc
     6001 gtaacgcgtg ttttcatgcg agctggcggg cgatgagccg ctctagcgtg tcttgggccc
     6061 gcatgatcag ctcgcagacc tcgcggacgg cgcctctgcc gccgggagcg cgcgtaacgt
     6121 ggtggactct ctctctcacg agctgcggcg cttcgggcac gctagcggca aaaccgcaac
     6181 gcgcgagcac cggcagatcc gcccaatcgt cgcccatgta tccggcggcg gcggccgagg
     6241 tgcgcaagcg gccgatcagg ttctcgaaag cggcgccctt gtttccggca ccttgttcca
     6301 cgagcgtgat gccaagctcg gcggcgcgga cctcgacggc gcgcgagcga cgcgcggaaa
     6361 gaatggcgac ctcgaccccg ctctccttca gcatcttgag gccatggccg tcctgagcgt
     6421 tgaacgcctt caactcggct ccagtctcgc tgaagtacag ggtcccgtcg gtgagcacgc
     6481 cgtcgacatc gaagatcatc aggcgcagcc gccgcattcg ctccagcacg tcgtcgcgca
     6541 tcaaatgacc ttggcgcgaa acaggtcgtg caggctgagg gcgccggcca gctcgccggt
     6601 ttccgcgacc accagcagtt gactgatcct gtgacggtcc atcaactcga cggcctccgc
     6661 ggcgagcttg tgcggggcga tcgtgcgcgg atcgcgtttc atgacatcgg taatcttgag
     6721 tccgcggatg tcgggtttgc tctccagggt ccggcgcaga tcgccgtcgg tgaatattcc
     6781 gacgagcttt ttcccgtggc cgaggatcgc cgtcatgccc atgcctttcc cggaaatctc
     6841 ggccagcatg gtggagaagg aatccgtgtc ggatacggcc ggaatgtctt tgcctgtccg
     6901 catgacctcg gatacgcgcg tgagaagctt tctgccgagc gcgccgccgg gatgcgcgcg
     6961 agcgaagtcc tgctcgtcga aaccgcgagc gtcgagcaaa gcgaccgcca gggcgtcgcc
     7021 gagcgcgagc gccgcggtgg tgcttgccgt cggcgcaaga ttcagagggc aggcctcctg
     7081 cgcgacgccc gcgtcaagat gcacatcggc ttcccgggcg agcgccgagt cgggattgcc
     7141 ggtcagcgcg atcagcttcg cgccctggcg ttttatccgg gggatgatcg tgagcagctc
     7201 gtccgactgt ccggaattcg agagcgccag caccacgtca tcgcgcgtga tcatgccgag
     7261 atcgccgtgt cccgcttcgg cggcgtgaac gaaaaaagcc ggtgtccccg tgcttgccag
     7321 cgtggatgcg atcttgcgag cgatgtgccc cgacttgccg acgccgctta cgacgactcg
     7381 cccgcggcac gcgaggatca gcccgaccgc atcgacaaaa cgctcatcga gtcgcgatat
     7441 caggccgctc accgcatcgg cctcgatcgt gagcaccttg cgcgcgagct caagcgcgga
     7501 agtcgaaatt gctttcttcg agcgatcgtt catcgagaga aaccgtgctg gcctgcgccg
     7561 ggagcagcct ttatcattag gctggagagt ataccagacc gattccgcac gttttcccac
     7621 gtgaacccga aaaaacaagg acttccggat gggcgacacg cttgaattgg tgctgatcct
     7681 gctggcgagc gcggtgttcg tcgtggtgct ctttcggcgg ctgcatttgc ctccgctgct
     7741 cggatatctc ctggtgggtg tcgcggtggg cccctttgcg ctggcgtgga ttcccgatac
     7801 cgccgaggga cggcatcttg cggaattcgg cgtcgtgttc ctgatgttct cgatcgggct
     7861 ggagttcagt ctgcccaagc tgtttcagct gcgccgcgcc gtgttcgggc tcgggcttgc
     7921 ccaggtcctg ctcacgctcg gtgccgtgct cgctgtcgcc atatttgcgg gggccccatg
     7981 gcaagccgcg ctcgcggttg ggggcgcgat cgccatgtcc tcgacggcga tggtcctgcg
     8041 cattctcgcc gagcgcatac agctggaaac ccctcacgga cgcgagatcg tcggcgtgct
     8101 gcttttccag gatctcgcgg tcgttccgtt gctgatcttg attcctgcga ttgcggaggg
     8161 gagcggcgag cttgcgcagc gcctggggtt cgcgctcgtc aaagccgcgg tggtgctcgt
     8221 cgtgctgctc tttgtcgggc agagactcat gcgcggctgg tttcacatcg tggcgcgccg
     8281 ccattcgcat gagctgttca ttctcaacgt cctgctcatc acgctggggc ttgcttggct
     8341 cacggaccgg gcgggactgt ctttggccct gggcgcgttt ctcgccggca tgctcatagc
     8401 cgaaaccgaa taccgccatc aggtcgagga ggacatcagg ccgtttcggg aggtcctgct
     8461 gggtctgttc ttcgtgacca tgggcatgag actcgacccg gggacggttg cggcgaattt
     8521 ttccctgacg ctggcgctga cggctctgct ggtggtgttg aagttcatcc tggttgccgg
     8581 cctcgcgcgc ctgttcggag ctggccccgg agcggccgtg cgcacggggc tggcgctcgc
     8641 ccaggtgggc gagttcggtc tggtcctcat ggtcctggcg cagtcggttg gattgctcga
     8701 tcgcgacttt tcccaggtgg tcgttgcggc gatgctgctc tccatgctcg ccgctccgtt
     8761 cctgattcag gcaagcgaca agctcgccct gcgctggtct agcgctgaat ggatgctgca
     8821 atcgctcgcg ctgcatcgcg tcgccgcgca gagtctcgcc accgagcgcc acgtcctcgt
     8881 atgcggctac ggccgcacgg gccagcgcct ggcgcacatg ctggagcagg agcacatcga
     8941 tgtcatcgcc ctcgacaccg atccggagcg cgtgcgcgga gcggaagccg cgggcgagaa
     9001 ggtcgttttc ggcgacgcgt cacggtacga aacgctcgtg gcggcaggtg tggcgcgagc
     9061 gagcgcgctc gtcattacct tcgcggatac gcagcttgcg ctgcgtatcc tgcatcacgt
     9121 gcgcgcgctc aatcctgctt tgccggtgat cgtccgtact ctggacgacg ccgacatgga
     9181 ccggcttatc gccgcggggg cgtcggaagt ggtccccgaa acgttcgagt cgagcttgat
     9241 gctcgcctcg cacgctttgg tgctgctggg agtgccgttg aggcgggtgg tgcgccgcat
     9301 tcgcgaagtg cgcgagcatc gatacagcct gatgcgcggg ttttttcatg gaggtaccga
     9361 cgaagccgaa gaccccgatg aggcgcgcga accccgcctg cattcggtga cgctcgcgac
     9421 gggcgcgagt gcctccggtc gcagcctggg cgagatcgcg ctggataagc tcggcgtgcg
     9481 cgtgacagcg ctgcggcggc gggagctgcg gatgatttcg ccggatccgc aaacggcatt
     9541 ggaggagggc gacgtgatcg tgctgctcgg cacgcctgag caactcgccg cggcggagat
     9601 gcgcctgctt gagggctagc cagtgcgggc ggaatccatc cgtcgggagc gaggcgagcg
     9661 cccgttactt tctcgagtcg gaagcggccc tggcgatggc gagcagaggc tccattgcgt
     9721 gtgggtcggt gagacgcgcc tgttcgagga tcaggagcg
//
LOCUS       DASPZD010000392         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1248060, whole genome shotgun sequence.
ACCESSION   DASPZD010000392 DASPZD010000000
VERSION     DASPZD010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248060"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /gene="lipB"
                     /locus_tag="VEM33_07610"
     CDS             complement(<1..403)
                     /gene="lipB"
                     /locus_tag="VEM33_07610"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HYS51731.1"
                     /translation="MTLVEEILVKQLGRVDYLPTLEAMRVFTARRNEATADEIWLLEH
                     PPVYTLGQGAERPSVDNGIPLAQADRGGEITYHGPGQVVLYALVDLARRGIKVRQFVA
                     MLENSVIELLAGRGERKLGAPGVYVGGAKIAA"
     gene            complement(400..672)
                     /locus_tag="VEM33_07615"
     CDS             complement(400..672)
                     /locus_tag="VEM33_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HYS51732.1"
                     /translation="MPVVADGSALAFPCDFPIKVMGRKEPRFAQTVMEIVLKHAPDFD
                     AATMEMRPSRQGRYLSLTCIVRATSREQLDALYRELCDHPSVVMVL"
     gene            complement(686..1555)
                     /locus_tag="VEM33_07620"
     CDS             complement(686..1555)
                     /locus_tag="VEM33_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HYS51733.1"
                     /translation="MVFLNGRILPIEDAKVSVLDRGFIFGDGVYELVPVYSRVPFRLE
                     EHLVRLARSLAQVRIRSPYSRAQWREFIYRLIDAQPFDDQGVYFQVTRGVAKRDHAFP
                     KNAEPTVFMMSNPLVNPPREQVEKGASAVSAPDNRWLRCDIKSISLIGNVLLRQLSAE
                     ADGAETILFRDGKLTEASASNVFIVRRGVIESPPKGNLILPGITYDVIAELARANGMP
                     LEFREVREPEVRGADELWVTSSSKEVLAIVMLDGKRIGDGQPGPVFRRMYQLYQEFKL
                     KVMRAGKREAVSV"
     gene            complement(1566..>2368)
                     /locus_tag="VEM33_07625"
     CDS             complement(1566..>2368)
                     /locus_tag="VEM33_07625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYS51734.1"
                     /translation="KGMEVQSGNDACIALAEAVAGSEDVFAQMMNREAARLGMKNTHF
                     MNSTGLPDAQHYSTAQDLYLLAAALIRDFPAEYAQYYSQKEFRYNNITQPNRNRLLWL
                     DPTVDGVKTGHTEAAGFCLIASSRRGGRRLLSVLLGSTSEATRAQESQKLLNWGFQFF
                     DSVKLYAGGDAVKSLEVWKGAGNAVKAGFKGDLMITVPKGEAGKLKAELLSQQPLVAP
                     VARGQRVGTLRVTHDGRPVGEYPVVALEPVAVAGIFGRAWDTLRLWFK"
BASE COUNT          401 a          820 c          738 g          409 t
ORIGIN      
        1 gcgccgcgat cttcgcgccg cccacgtaga cgcccggtgc tccaagcttg cgttcgccgc
       61 ggccggcgag aagctcgatc accgagtttt cgagcatcgc aacaaactgc cttaccttga
      121 tgccgcgccg cgccaggtcc accagcgcgt agaggaccac ctggcccggc ccatggtagg
      181 tgatctcgcc gccgcgatcg gcctgggcga gcggaatgcc gttatcgacg ctcggccttt
      241 cggcgccctg tcccagggtg tagaccggcg gatgttcgag cagccagatc tcgtccgcag
      301 tggcctcgtt tctgcgcgcg gtgaacacgc gcatcgcttc gagcgtcggc agatagtcga
      361 cccgccccag ctgcttgacc agaatttcct cgaccagcgt catagcacca tcactaccga
      421 ggggtgatcg cacaactccc ggtacagcgc gtcgagctgc tcgcgcgagg tggcgcgcac
      481 gatgcaggtg aggctgaggt agcgtccctg ccggctcgga cgcatctcca tcgtcgccgc
      541 gtcgaaatcc ggtgcgtgct tgagaacgat ctccatcacg gtctgcgcga agcgcggctc
      601 cttgcgtccc atcaccttga tcgggaagtc gcaaggaaaa gcgagcgctg agccatccgc
      661 taccaccggc atgagtcgcc gcccttcaga ccgacactgc ctcgcgtttg cccgcgcgca
      721 tcaccttcag cttgaactcc tggtagagct ggtacatccg ccggaaaacg gggccgggct
      781 ggccgtcgcc gatcctcttg ccgtccagca tgacgatcgc cagcacttcc ttcgacgagg
      841 acgtgaccca cagctcatcg gcgccgcgca cctccggctc gcggacttcc ctgaattcga
      901 gcggcatgcc gttcgcgcgt gcgagctcgg cgatcacgtc gtaggtgatt cccggcagga
      961 tcaggtttcc cttcggcggc gactcgatga cgcctcgccg gacgatgaag acgttcgaag
     1021 ccgaagcctc ggtcagcttg ccgtcgcgaa acaggatcgt ctcggcgccg tccgcctcgg
     1081 cggaaagctg gcgcagcagc acgttgccga tgagcgaaat cgacttgatg tcgcagcgca
     1141 gccagcgatt gtccggggcg ctcaccgcgg aggccccctt ctccacctgc tcccggggcg
     1201 ggttgacgag cggattcgac atcatgaaca ccgtcggctc cgcattcttc ggaaaggcgt
     1261 gatcgcgctt ggccacgccg cgcgtcacct ggaagtacac gccctggtcg tcgaacggct
     1321 gcgcatcgat caggcggtag atgaactcgc gccattgcgc ccgcgagtac gggctgcgga
     1381 tgcgcacttg cgcaaggctg cgcgcgaggc ggaccagatg ctcctcgagc cggaacggca
     1441 cccgcgagta caccggcacc agctcgtaga ccccgtcgcc gaagatgaac ccccggtcga
     1501 gcaccgaaac cttcgcgtcc tcgatcggga gaatcctgcc gttcagaaaa accatgtgcg
     1561 cctcgctact tgaaccacag cctgagtgta tcccaggccc gcccgaaaat cccggcgaca
     1621 gccaccggct cgagagcgac cacggggtat tcgccgaccg gtctgccatc gtgcgtgacg
     1681 cgcagcgtgc cgacgcgctg gccccgtgcc accggcgcga cgagcggctg ctgcgacagc
     1741 agctcggcct tcagcttgcc cgcctcgcct ttcggcacgg tgatcatcag gtcgcccttg
     1801 aagcctgcct tgaccgcgtt tcccgccccc ttccagacct cgagcgattt cacggcgtca
     1861 ccgccggcgt agagcttcac gctgtcgaag aactggaagc cccaattgag cagcttctgc
     1921 gactcctgcg cgcgcgtcgc ttccgacgtc gagccgagca ggaccgagag caggcgcctg
     1981 ccgccgcgcc gcgacgacgc gatgaggcag aagccggccg cctcggtatg gccggtcttg
     2041 acgccgtcca ccgtcggatc gagccacagc agccggttgc ggttcggctg cgtaatgttg
     2101 ttgtagcgga attccttctg cgaatagtac tgggcgtact cggccgggaa gtcgcggatc
     2161 aacgccgcag cgagcaggta caagtcctgc gccgtgctgt aatgctgcgc gtcgggcaag
     2221 ccggtggaat tcatgaagtg ggtattcttc atgccgagac gcgccgcctc gcggttcatc
     2281 atctgcgcga agacgtcctc ggagccggcg accgcttcgg cgagagcgat gcacgcgtcg
     2341 ttgccggact ggacctccat gcccttga
//
LOCUS       DASPZD010000393         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_38534, whole genome shotgun sequence.
ACCESSION   DASPZD010000393 DASPZD010000000
VERSION     DASPZD010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38534"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..881)
                     /locus_tag="VEM33_07630"
     CDS             complement(12..881)
                     /locus_tag="VEM33_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HYS51735.1"
                     /translation="MLTRIAVAVAWMLHRLPLAVLAPIGSALGSLLYILGRERRKVCL
                     TNLSRCLPEMTERERVALAKAHFRAFGRSVLERSILWWGSRERVMRLIRIVGLERVSA
                     LKGQPLILLAPHFVGLDAGCTRLTCEIDMAGIYAKQKNAFFDALLLSGRTRFGHQLAI
                     SRQEGVRPALAALKRGIPFYYLPDQDYGPRDAIFVPFFAASAATITGLSRMTRLTGAR
                     VLPCVTRMLPGGAGYELSCYPIWENFPSGDAAADARRMNAFIEERVREMPEQYFWTHK
                     RFKTRPPGEAQWY"
     gene            complement(895..1734)
                     /locus_tag="VEM33_07635"
     CDS             complement(895..1734)
                     /locus_tag="VEM33_07635"
                     /inference="COORDINATES: protein motif:HMM:NF006487.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYS51736.1"
                     /translation="MLFLLRLAARLPLPLLHALGAALGWTVYICSPRYRRDFKRNLAL
                     AGLSDFRRAAVPEAGKSVMEVPAVWLRPLGRVAGLVVEVNGWEHMEQGAKRGKGVIVV
                     TPHIGCWEMVGQYVASRIPITVMYRTPKIRVLEPLMRIGRSRGPAMKSITADLRGVRA
                     MLKALKRGEAIGLLPDQVPGIGEGEWVEFFGRPAYTMTLVGRIAEQTGAPVLLCCAER
                     LPRGRGYRFFAEPVLAPRPPESAVRALNRSLERLIRRHPEQYLWGYNRYKVPAGVLPP
                     DAR"
     gene            1821..>2380
                     /locus_tag="VEM33_07640"
     CDS             1821..>2380
                     /locus_tag="VEM33_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HYS51737.1"
                     /translation="MSSYLFTSESVSEGHPDKVADQISDAVLDAILAQDKTSRVAAET
                     LVKDNMVVLAGEITTGARVNFSEVVRKTVKRIGYNDPTIGFDTHTCTVIVAYGEQSQN
                     IAQGVDEGKGLDLEQGAGDQGLMFGYACDETPQLMPMAIHLAHRLVERQSEIRRDGRL
                     SWLRPDAKSQVTIKYVDGKPDSIDTVV"
BASE COUNT          455 a          786 c          762 g          377 t
ORIGIN      
        1 atggcgaagg atcaatacca ttgggcctcc cccggcggtc gggtcttgaa gcgcttgtga
       61 gtccagaagt actgctccgg catctcgcgc acgcgctcct cgatgaaggc gttcatgcgg
      121 cgcgcgtcgg cggcggcgtc gccgctcggg aaattttccc agatcggata acacgacagc
      181 tcgtatccgg cgccgcccgg cagcatgcgc gtcacgcagg gcagcacgcg ggcgccggtg
      241 agccgcgtca tgcgcgaaag cccggtgatc gtcgcggcgg aggccgcgaa gaacggcacg
      301 aaaatcgcat cgcgcggccc gtagtcctga tcgggcagat aatagaacgg aatcccccgc
      361 ttcaacgcgg caagcgccgg gcgcacgcct tcctggcggg aaatcgcgag ctggtgcccg
      421 aagcgcgttc gccccgagag gagaagggcg tcgaagaaag catttttctg ttttgcgtag
      481 atccccgcca tgtcgatttc gcaggtgagg cgggtgcagc ccgcatccag gccgacaaag
      541 tgcggtgcga gcaggatcag cggctgcccc ttgagcgcgc tcacccgttc gagtccgacg
      601 attcttatga gccgcatcac gcgctcgcgt gatccccacc acagaatcga gcgctcgagg
      661 acgctgcggc cgaaggcgcg gaagtgcgct ttggcgagcg cgacacgctc cctttccgtc
      721 atctccggca agcagcggga aagattggtc agacagacct tgcgccgctc gcgtccaagg
      781 atgtacagga gcgaacccag agcggagccg atcggagcga gcacggcaag gggcaagcga
      841 tgcagcatcc aggcgacggc aacggcaatg cgggtcagca aggcgggctc ccctctaccg
      901 ggcatccggc ggcagcactc ccgccggcac cttgtagcgg ttgtagcccc acaggtactg
      961 ctcgggatgg cggcgaatca gccgctccag cgagcggttg agggcccgta ccgccgattc
     1021 gggcggccgc ggcgcgagca ccggttccgc gaagaaacga tagccgcgcc ccctgggcag
     1081 gcgctcggcg caacacagca gcaccggagc cccggtctgc tcggcgatcc ggccgacgag
     1141 cgtcatggtg tacgcgggcc tgccgaagaa ctcgacccat tcgccttcac cgatacccgg
     1201 cacctgatcg ggcagcagcc cgatcgcttc tccgcgtttg agtgccttca acatcgcgcg
     1261 caccccccgc agatcggcgg taatgctttt catcgcgggg ccgcggctgc ggccgatccg
     1321 cataagcggc tcgagcaccc ggattttggg cgtgcggtac atcaccgtga tcggtatgcg
     1381 gctcgcgacg tactgtccca ccatttccca gcagccgatg tggggcgtca cgacgatgac
     1441 gccctttccg cgtttcgcgc cttgctccat atgctcccag ccgttcacct cgaccacgag
     1501 gccggccacc cgccccagcg gacgcagcca gaccgccggc acttccatga cgctcttgcc
     1561 cgcctcgggg acggcggcgc gccgaaaatc gctcagccct gctagagcga gattgcgctt
     1621 gaaatccctg cggtaacgcg gtgagcagat atagaccgtc catcccagcg ccgcgcccag
     1681 agcgtgtaac agcggcaggg ggagacgcgc cgccagccgc agcaggaata gcatgtgccg
     1741 aaagtgggat ggtgtgggtt gggtgggcga aatcgctgtg ctagactggc aaaccttttt
     1801 tcgcagggaa aaccgccagc atgagcagct atctattcac gtccgaatcg gtgtctgaag
     1861 gacatccgga caaggtcgcc gaccagattt ccgatgcggt tctggacgcc attctagccc
     1921 aggacaaaac ctcccgagtc gccgccgaga ccctggtcaa ggacaatatg gtggtgctcg
     1981 ccggcgaaat caccaccggc gcgcgggtca atttctccga agtggtgcgc aagaccgtca
     2041 agcgcatcgg ctacaacgat ccgaccatcg gcttcgatac ccacacctgt acggtgatcg
     2101 tggcctacgg cgaacagtcg caaaacattg cccagggcgt ggacgagggc aaaggtctgg
     2161 acctcgagca aggcgcggga gaccagggcc tcatgttcgg ctatgcatgc gacgagaccc
     2221 cgcaactgat gccgatggcc attcatctcg cgcaccgcct ggtcgagcgc cagtcggaga
     2281 tccgccgcga cggccgcctg tcctggctac gcccggacgc caagtcccag gtcaccatca
     2341 agtatgtcga tggcaagccc gattcgatcg acacggtggt
//
LOCUS       DASPZD010000394         5398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1648022, whole genome shotgun sequence.
ACCESSION   DASPZD010000394 DASPZD010000000
VERSION     DASPZD010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648022"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VEM33_07645"
     CDS             complement(<1..1208)
                     /locus_tag="VEM33_07645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYS51738.1"
                     /translation="MIKYLSMAGGDPEQAARLRRFMLASAAYAVCAPLVWLASKFNLV
                     AQKPAWILVAMMVVVNVVLYSVFRTGLNRKFSDPNLTWLQVFVGNVMVMYAVYSFDQG
                     RAVVLNLSLVVLTFGVFYFTTREFVKAALQILAGYAAVINLLMFFKPETVNVYHEWFQ
                     WAGLAAVLPLFAVIGGRISGLRQRLGASNEELRSALGNVRQIATHDQLTGLPNRVLFN
                     EELQRALARAERHARPVGLFFMDLDRFKNINDTLGHQFGDRVLQEAAKRLASCVREGD
                     IIARLGGDEFVLLVEELGDPAALTEIARKLLASVSELRKIDGQELNVSLSIGICAYPA
                     DGRDAKTLLSGADIAMYRAKDRGRNGFCFYSAELQSHTPEKLALEAGLRHGLERGEFR
                     VYYQPKIDMTT"
     gene            1467..2618
                     /locus_tag="VEM33_07650"
     CDS             1467..2618
                     /locus_tag="VEM33_07650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYS51739.1"
                     /translation="MRHIPPMRLGYFTMPLHPPHRDPAQTLQEDREAVVLADRLGFHD
                     AFVGEHLTDRCENVTNSFIFLATLIGETKTIKLATGTSNLSQSHPVVIAAQAAMFDHL
                     AKGRFIFGVSPGALSSDAEALGILAEDRNRMFAEAIDVILAIWERDPPYDIDLPGNRY
                     KVTTAKSHVPEIGRGVMYKPYQKPRPEIVGTVVAPRSKGVIAMGERDFHPLSANFLLP
                     HWLPSHWENYAEGKRRAGKTPDPAEWRIARTIFVSDDDRVAERYAREDPRSPYRFYWK
                     QLREKMLRAGRQVIFKLRENEDDGAVTLERLLAELVLCGTVDKVVDQLLALRERSGDF
                     GEIVYAGMDWVDPALARRSMELMANEVMPRVNKAVGGAAKEPPREAVRI"
     gene            2705..3472
                     /locus_tag="VEM33_07655"
     CDS             2705..3472
                     /locus_tag="VEM33_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYS51740.1"
                     /translation="MEDESTIQKNIADKLIAALKTGGFVLYAQKILPLSGDAPPFQEI
                     LVRFKEEEEKLLPPGTFFPMLQEYRLLPYVDRWVVSRLASWVQESRARTTPGSPVPAN
                     GVNLSEDTLREPKFADFVAKNIESAKLPEGFFTFELGWDTALLHADQLKNIQVQLAPM
                     GCRFTFAGFDGSEGSFSFLKVLKPDFVKLTYGVVKDVGRGLAAAEKVEGINQKCHAMG
                     IKTIAEFVESHEVLDALRLIEVDFAQGLIIGGPQQLT"
     gene            complement(3496..4008)
                     /locus_tag="VEM33_07660"
     CDS             complement(3496..4008)
                     /locus_tag="VEM33_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51741.1"
                     /translation="MTRIGATLVVAALATAASAPAAAADVAGVFSQGRTHLAVVGGSG
                     YAFDKSYLVLGVGASYYLADGLNIGLYAESWTGSDPSMYKLTPSVQYVFHQVPRVSPY
                     VGVFYRRTYIDNLPNLDSAGGRAGVYIAAGGNAHVSGGVVYESYLDCNKAVYKSCSDT
                     YPEIGITFAF"
     gene            complement(4008..4952)
                     /locus_tag="VEM33_07665"
     CDS             complement(4008..4952)
                     /locus_tag="VEM33_07665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HYS51742.1"
                     /translation="MKRTAVAIAFLAAPTLAFAQWNVPSESQRCPSKWGAGDERGSGN
                     HMKNPEVVLRGARLIKTGEVIELGHVLGPGMAFFGTRIFNLHTKRTFMNTGRNTRGSN
                     EEVVTSEIGQVGTQFDGFAHQSHGDSHYNCFKTSEIATRSGFTKLGVQNAPTFFARGV
                     MLDVAALKGVEMLGDTYEITVADLQQALDRQKLKLLPGDAIIIHTGWGKLYGKDNARF
                     VKSTPGVGVAAAEWLAKQDPLLVGSDNWPVEVAPNPDKDLSLPVHQIFLVVNGIHILE
                     NLKLDELAAKRVYEFAFTMQPLKIHGASGSSVAPAAIR"
     gene            complement(4962..>5398)
                     /locus_tag="VEM33_07670"
     CDS             complement(4962..>5398)
                     /locus_tag="VEM33_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HYS51743.1"
                     /translation="WKDKAAWDAHMAAAGTKEFREKLNALRNAPADDRFHNALSVGPM
                     EVAPGSVYGVTHVDVIPPQKDNAIVALKVLGEANRAAAGNVRFEIVQQTNRPNHFTVF
                     EIWRSREAFDANGMSAHQREFRDKLAGMAGALYDERLYETLN"
BASE COUNT          947 a         1724 c         1773 g          954 t
ORIGIN      
        1 gtcgtcatgt cgatcttggg ctggtagtac acgcggaact cgccccgctc caggccgtgc
       61 cgcaggccgg cctccagcgc gagcttctcc ggcgtgtgcg attgcagctc ggcggagtag
      121 aaacagaatc cgttgcggcc ccgatccttg gcgcggtaca tggcgatgtc cgctcctgag
      181 agcagcgtct tcgcgtcgcg gccgtcggcc ggataggcgc agatcccgat gctcagcgag
      241 acgttgagct cctgcccgtc gatcttgcgg agctccgaca cggaagcgag cagcttccgc
      301 gcgatctcgg tcagcgcggc cggatcgccc agctcctcga ccagcagcac gaactcgtct
      361 ccgccgagac gcgcgatgat gtcgccctcg cgcacgcaac ttgcgaggcg cttggcggct
      421 tcctgcagca cccggtcgcc gaactggtgg ccgagcgtgt cgttgatgtt cttgaagcgg
      481 tcgaggtcca tgaagaagag cccgaccggc ctcgcgtgac gctccgccct ggcgagcgcg
      541 cgctggagct cctcgttgaa caacacccgg ttgggcaggc ccgtgagctg gtcgtgcgtg
      601 gcgatctgac gcacgttgcc gagggccgag cgcagctcct cgttgctcgc gcccaggcgc
      661 tggcgcagac cgctgatcct gccgccgatg accgcgaaca gcggcagcac ggcggcgagc
      721 cccgcccact ggaaccactc gtgatagacg tttaccgtct ccggcttgaa aaacatgagc
      781 agattgatca ccgccgcgta gccggcgaga atctgcagcg cggccttgac gaactcgcga
      841 gtggtgaagt agaacacgcc gaaagtcagc acgaccaggc tcaggttgag cacgaccgcg
      901 cgcccctggt cgaagctgta gacggcgtac atcaccatga cgttgccgac gaagacctgc
      961 agccaggtca ggttcgggtc gctgaatttc cggttgaggc cggtgcgaaa cacagagtag
     1021 aggacgacgt tcaccacgac catcatggcg acgaggatcc aggcgggctt ctgcgcgacc
     1081 aggttgaatt tgctggcgag ccacaccagg ggggcgcaga ctgcgtaggc ggcagaagcg
     1141 agcatgaagc gtcgcagccg cgccgcctgc tcgggatccc ctcccgccat ggacaagtac
     1201 ttgatcacgg tcgattcatc gagtcgtatc cccgtcctga cttgagcgga gcgaggctcg
     1261 ccccggcccg gaccgcggga gaatgcctgt actgccggcg ccggaaccgc ccctgccggc
     1321 tccgggtatc ccgaatatcg cgtgtccctg tgtctgaggg ggggcatggg tctcctggta
     1381 tcccgaagcc cgcattgtcg aaggctcgcg cgattctaac gtacccgggg aaaggagagg
     1441 cgatcgcgtt tgactgccgc gccgcgatgc ggcacattcc gcccatgcga ctgggctact
     1501 tcacgatgcc gctgcatccg ccgcaccgcg atccggcgca gacgctgcag gaggaccgtg
     1561 aggcggtcgt cctcgcggac aggcttggat tccacgacgc cttcgtcggt gagcacctca
     1621 ccgaccgctg cgagaacgtc accaacagct tcattttcct cgcgacgctg atcggcgaga
     1681 ccaagacgat caagctcgcg accgggacgt ccaacctctc gcaatcgcac ccagtggtga
     1741 tcgccgctca ggcggcgatg ttcgaccacc tcgcgaaggg acgcttcata ttcggcgtca
     1801 gcccgggggc gctctcctcg gacgccgagg cgctcggcat tctggccgag gaccgcaacc
     1861 gcatgttcgc cgaggcgatc gacgtgatcc tcgccatctg ggagcgcgat ccgccttacg
     1921 acatcgatct gccgggaaac cgctacaagg tcacgacggc aaagagccac gtcccggaaa
     1981 tcggccgcgg cgtgatgtac aagccctacc agaagccgcg tccggagatc gtcggcacgg
     2041 tggttgcgcc ccgttccaag ggtgtgatcg ccatgggcga gcgcgatttt catccgctgt
     2101 cggcgaattt tcttcttccg cattggctgc ccagccactg ggagaactac gccgaaggca
     2161 agcgccgcgc cggaaagaca cccgatcccg cggagtggcg cattgcgcgc acgatcttcg
     2221 tctccgacga cgaccgggtc gccgagcgct acgcgcgcga ggatccccgc agcccctacc
     2281 gcttctactg gaagcagctg cgcgagaaga tgctgcgcgc cgggcgtcag gtcattttca
     2341 agctgcgcga gaacgaggac gacggcgcgg tgacgctcga gcggctgctg gcggagctcg
     2401 ttctctgcgg gacggtcgac aaggtcgtgg accagctgct cgcgctgcgc gagcgctcgg
     2461 gcgacttcgg cgaaatcgtt tatgccggga tggactgggt ggacccggca ctcgcgcgcc
     2521 gctcgatgga gttgatggcg aacgaggtga tgccgcgggt caacaaggcg gtcggcggcg
     2581 cggcgaagga gccgccgcgc gaggcggtgc gaatctgagc gatcccgcag gaacaattct
     2641 tgcgtctttc cgtccttggg gaaaacccct gaatcgggcc cgacggcgca atatgacagt
     2701 gcagatggaa gacgagagca cgatacagaa gaacatcgcc gacaagctga ttgcggcgtt
     2761 gaagacgggc ggtttcgtgc tctacgccca gaaaatcctg ccgctctccg gagacgcacc
     2821 gccgttccag gagattctcg tccgcttcaa ggaggaagag gagaagctgc tcccgccggg
     2881 aacgttcttt cccatgctgc aggagtaccg gctgctgccg tacgtcgatc gctgggtcgt
     2941 gagccgcctt gcgagctggg tgcaggaatc ccgcgcccgg acgacgcccg gctcgccggt
     3001 gcccgcgaac ggcgtcaacc tttcggagga cacgctgcgc gagcccaaat tcgcggactt
     3061 cgtcgccaag aacatcgaga gcgcgaagct tcccgaaggg ttcttcactt tcgagctcgg
     3121 ctgggacacc gccctgctgc acgccgacca gctgaagaat atccaggtgc agctggctcc
     3181 gatgggctgt cgtttcactt tcgccggttt cgacggcagc gagggctcgt tcagcttcct
     3241 caaggtgctc aagcccgatt tcgtgaagct cacctacggc gtcgtgaaag acgtcgggcg
     3301 tggcctcgcg gccgccgaga aggtcgaagg catcaaccag aagtgtcacg ccatggggat
     3361 caagaccatc gcggaattcg tcgagagcca tgaagtgctc gacgccctgc gcctgatcga
     3421 ggtggatttc gcccaggggc tcatcatcgg cgggccgcaa cagctcacct agcgccgcag
     3481 gacccgcggc gcacctcaga acgcgaaggt aataccgatt tccgggtagg tgtcagagca
     3541 acttttgtag acggccttgt tgcaatcgag gtaggactcg tacacgaccc cgccactcac
     3601 gtgggcgttg ccccctgcgg cgatgtagac accggcgcgg ccgccggccg aatcgaggtt
     3661 tggcaggttg tcgatgtagg tgcggcgata gaacacaccg acgtagggcg atacacgcgg
     3721 tacctggtgg aacacgtatt gcaccgacgg cgtgagcttg tacatgctgg gatcgctgcc
     3781 ggtccaggat tcggcgtaca gaccgatgtt gagaccgtcc gccagataat agctggcacc
     3841 tacacccaga acgaggtagc tcttatcgaa ggcgtaaccg ctgcctccga ccaccgccag
     3901 atgggtgcgc ccctggctga atacgccggc gacatcggcg gccgcggccg gcgcactggc
     3961 cgccgtggcg agggctgcga cgacgagggt ggcgccgatc ctggtcatca gcggatcgcg
     4021 gccggagcca ccgacgaacc ggacgcgcca tggattttca gcggctgcat ggtgaacgcg
     4081 aactcgtaga cacgcttcgc cgccagctcg tcgagcttga ggttctccag gatgtggatg
     4141 ccgtttacca ccaggaagat ctggtgcacc ggcaggctca ggtccttgtc ggggttgggc
     4201 gccacttcca ccggccagtt gtcagagccg acgagcagcg gatcctgctt cgcgagccat
     4261 tctgccgccg cgacccccac gcccggggtc gatttcacga agcgcgcgtt gtccttcccg
     4321 tagagctttc cccagccggt gtggatgatg atcgcatcgc ccggcaggag cttcagcttc
     4381 tgccgatcga gcgcctgctg caggtcggcc acggtgattt cgtaggtgtc gccgagcatc
     4441 tccacgcctt tgagcgccgc gacgtcgagc atcactcccc gtgcgaagaa agtcggcgcg
     4501 ttctgcacgc cgagcttggt gaagcccgag cgcgtcgcga tctcgcttgt cttgaagcag
     4561 ttgtagtgag aatcgccgtg cgactgatgc gcgaagccgt caaactgggt tccgacctgg
     4621 ccgatttcac tggtcacgac ttcctcgttg ctgccgcgcg tgttccttcc ggtattcatg
     4681 aaagtgcgct tggtgtgcag attgaagata cgcgtgccga agaacgccat gccgggtccg
     4741 agcacgtgcc cgagctcgat cacctcgccg gtcttgatga gccgcgcgcc gcgcagcacg
     4801 acctcgggat tcttcatgtg attgccagag ccgcgctcgt cgcccgcgcc ccatttcgac
     4861 gggcagcgct ggctttcgga gggtacgttc cattgcgcaa atgcgagcgt gggagcggca
     4921 aggaaggcga ttgcgacggc ggtgcgtttc atgtgagtcc cctagttgag agtttcgtac
     4981 aaacgttcgt cgtagagcgc gccagccatc ccggcgagct tgtcgcggaa ctcgcgctgg
     5041 tgcgcggaca tgccgttcgc gtcgaaggcc tcgcgcgagc gccagatctc gaacacggtg
     5101 aagtggttcg ggcggttggt ctgctgcacg atctcgaagc gcacgttgcc ggccgcggcg
     5161 cgatttgcct cccccagcac cttgagcgcg acgatggcgt tgtctttctg cggcgggatc
     5221 acgtcgacgt gggtcacgcc gtagaccgag ccgggggcga cttccatcgg accgacggag
     5281 agcgcgttgt ggaagcggtc gtcggccggc gcgttgcgca gtgcgttgag cttttcgcga
     5341 aactctttcg tgcccgcggc ggccatatgc gcgtcccagg cggccttgtc tttccagg
//
LOCUS       DASPZD010000395         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_530673, whole genome shotgun sequence.
ACCESSION   DASPZD010000395 DASPZD010000000
VERSION     DASPZD010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530673"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VEM33_07675"
     CDS             <1..435
                     /locus_tag="VEM33_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HYS51744.1"
                     /translation="DYNAIMLKALADRLAEAFTELLHERVRKEYWRYAADEKLSSTEL
                     ISEKYRGIRPAPGYPACPDHTEKAALFELLSAPEAGITLTESYAMLPAASVSGFYLSH
                     PDSRYFAVDKIGEDQAADYARRKALDLEQTERWLAPNLAYEP"
     gene            436..1296
                     /gene="metF"
                     /locus_tag="VEM33_07680"
     CDS             436..1296
                     /gene="metF"
                     /locus_tag="VEM33_07680"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HYS51745.1"
                     /translation="MQSQKKHLRAFSFEFFPPKTPEGMDKLRATWKQLAQLKPRFFSC
                     TYGAGGSTRDRTLETVLEIQAAGHRAAPHISCIGATHADVAATLELYKSKGIAHIVAL
                     RGDLPSGAASAGELRYASELVAFIRERTGDHFHVEVACYPEFHPQARSPHEDLRNFKR
                     KVDAGADAAITQYFYNIDSYFRFVDDCEALGIDLPIVPGIMPIGNFSQLARFSDACGA
                     EIPRWLRLKMQSLGDDVASIRSLGLDVVTDLCDRLLSAGAPGLHFYTMNQAGLTSTIW
                     ERLRIEPGAA"
     gene            complement(1297..2109)
                     /locus_tag="VEM33_07685"
     CDS             complement(1297..2109)
                     /locus_tag="VEM33_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HYS51746.1"
                     /translation="MERKGNSRSRTRLSSVANSLRLIKAFSEDEYEIGISDLAKRLGL
                     AKSTVHRLASTLLEQGILEQNSGDGKYHLGLALFELGAMVRRKMDFTMEARPFLRTLM
                     EKTGETVHLAILDRDSILYIITHESKQALRMGSKVGTRAPVHSTAVGKALLAFLPEDE
                     LERIIARGLPASTPRTIVDARALRRELATVRTRSYAVDDEESEIGLRSIAAPIRIYSG
                     NVVAAISIAGPVHRMARKALLGWVRELVDAAEAVSQRLGWQPSRAEALPRRA"
BASE COUNT          372 a          759 c          678 g          366 t
ORIGIN      
        1 gattacaacg ccatcatgct caaggctctc gccgaccggc tcgccgaagc ttttacggag
       61 ctcctgcacg agcgcgttcg gaaggagtac tggcgctacg ccgcggacga gaaactttcg
      121 agcaccgagc tgatctccga aaaataccgc ggcatccgcc ccgcgcccgg ctacccggcg
      181 tgtcccgacc acacggagaa ggcggcgctg ttcgagctgc tctccgctcc ggaggccgga
      241 atcacgctca cggaatcgta tgccatgctt cccgccgcga gcgtgagcgg cttttatctc
      301 tcccacccgg actcccggta tttcgcggtg gacaagatcg gcgaggatca ggctgccgac
      361 tacgcccggc gcaaggcgct cgacctggag cagaccgagc gctggcttgc ccccaacctc
      421 gcctacgaac cctgaatgca atcccagaaa aagcacctgc gcgccttcag cttcgagttc
      481 ttcccgccga agaccccgga agggatggac aagctgcgcg cgacctggaa gcagctcgcg
      541 cagttgaagc cgcgcttctt ctcctgcact tacggcgcgg ggggctccac gcgcgaccgc
      601 acgctcgaga ccgtcctcga aatccaggcc gccggacacc gcgctgcgcc ccacatctcc
      661 tgcatcggcg caacgcacgc cgacgtcgcc gccaccctcg agctgtacaa gtccaaaggc
      721 atcgctcaca tcgtcgccct gcgcggggac ctgccttcgg gcgcagccag cgcgggagag
      781 cttcgctacg cgagcgagct ggtggctttc atccgcgagc gaaccggcga tcacttccac
      841 gtggaagtcg cctgctatcc ggagttccat ccgcaggcgc gcagcccgca cgaggacctg
      901 cggaatttca agcgcaaggt cgacgcgggg gcggacgctg cgatcacgca gtatttctac
      961 aacatcgatt cctatttccg cttcgtcgac gactgcgaag cgctgggcat cgacctgccc
     1021 atcgtccccg gaatcatgcc gatcgggaat ttctcgcagc ttgcccgctt ctcggacgcc
     1081 tgcggcgccg aaattccgcg ctggctgcgc ctgaagatgc agagtctcgg cgacgacgtc
     1141 gcctcgattc gaagcctggg cctggacgtg gtgaccgact tgtgcgaccg gctgctctcc
     1201 gccggagcgc ccggactgca cttctacacc atgaaccagg cggggctcac ctcgacgatc
     1261 tgggagcggc tgaggattga gcccggcgcg gcgtgatcag gcgcgcctgg gcagagcctc
     1321 cgcgcgcgac ggctgccagc cgagccgctg cgaaaccgcc tcggccgcgt ccaccagctc
     1381 gcgcacccag cccagcagcg cctttctcgc catgcggtga accggaccgg cgatgctgat
     1441 cgccgcaacc acgttgcccg agtagattcg tatcggggcg gcgatggagc gcaatccgat
     1501 ctcgctctcc tcgtcgtcga cggcgtagct gcgcgtccgc acggtggcca attcgcggcg
     1561 cagagccctg gcatcgacga tcgtcctggg cgtgctcgcc gggagcccgc gggcgatgat
     1621 gcgctcgagc tcgtcctccg gaaggaacgc gagcaaggcc ttgcctacgg cggtggagtg
     1681 cacgggcgcg cgggtgccga ctttcgaacc catgcgcagc gcctgcttgc tctcgtgcgt
     1741 gatgatgtac aggatcgaat cgcgatcgag aatcgcgaga tgcacggtct cgccggtctt
     1801 ctccatgagc gtgcgcagga aaggcctcgc ttccatcgtg aagtccatct tccggcgcac
     1861 catcgccccc agctcgaaca gagcgagccc gaggtgatat ttgccgtcgc cggagttctg
     1921 ctcgaggatc ccctgctcga gcagcgtcga ggcgaggcgg tgaacggtgc tcttcgcgag
     1981 cccgagccgc ttggcgagat cgctgatgcc gatctcgtac tcgtcttctg aaaacgcctt
     2041 gatgagccgc aaggaattcg ccaccgacga cagccgggtg cgggatctgg aattgccttt
     2101 tcgctccatc gccggggttc tcaggcggcc gcccgacgcg ccagccgcat tgcgatggcg
     2161 aaactcgcga ccgcg
//
LOCUS       DASPZD010000396         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_761922, whole genome shotgun sequence.
ACCESSION   DASPZD010000396 DASPZD010000000
VERSION     DASPZD010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761922"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /gene="hflC"
                     /locus_tag="VEM33_07690"
     CDS             <1..785
                     /gene="hflC"
                     /locus_tag="VEM33_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HYS51747.1"
                     /translation="IVFQLGEIKDLIDEPGLYFKFPLIQNVRYFDKRILTLDTADTER
                     FITSEKKNVLVDSFVKWRIQDMRQYYISVQGDEQRAQLQLSQTVKAALQDEFSKRTVH
                     DVISGERDKIMQVVREKVDEDMKKIGMEIVDVRLKRVDLPQEVSESVYRRMDAERKSV
                     AAELRSQGFSAAEKIRAEADREREIIIAGAYRDAQRVKGEGDAKAASVYARAFNENPE
                     FYAFYRSLDAYRASFKNKSDVIVLDPNSEFFKYFRNSGKGGK"
     gene            786..971
                     /locus_tag="VEM33_07695"
     CDS             786..971
                     /locus_tag="VEM33_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HYS51748.1"
                     /translation="MGTTFIMAFALMLVIEGLLPFLLPTLWRNTFRRITEFTDGQIRF
                     FGLSSMLVGLLILFFIR"
     gene            996..2150
                     /locus_tag="VEM33_07700"
     CDS             996..2150
                     /locus_tag="VEM33_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HYS51749.1"
                     /translation="MRNWLLPEKFEDILPPQARRIDRMRARLLELFRVHGYELVIPPM
                     LEYLESLLTGTGHDLDLRTFKLVDQLSGRMMGVRADIAPQVARIDAHLLNRKGVARLC
                     YAGSVLLTRPRGLDSTREPLQVGAEIYGHPGIESDLEIQQLLAESLAACGISGARLDI
                     GHVAVFRAICRHGGVGAELESELYEALEGKDLPALRAVTRSMPEATSKALLALPELYG
                     DGTVLERARRVLPKYPEIDAALGDLEVLSRSGSIPVSVDLADLRGYQYHSGVVFAAYA
                     PGVANAAALGGRYDEVGKAFGRARPATGFSMDLRDLARTAPHEAEAAAIRAPYSSDAG
                     LATAIRKLRIAGEVVVVGLPGHAAEDAQPDCDRELVKQAGEWTVRKLGKS"
     gene            2155..>2440
                     /locus_tag="VEM33_07705"
     CDS             2155..>2440
                     /locus_tag="VEM33_07705"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HYS51750.1"
                     /translation="MAKNVVVIGTQWGDEGKGKVVDWLTDHAQGVVRFQGGHNAGHTL
                     VIGGKKTVLHLVPSGILRNGVTCYIGNGVVLSPQALIDEIDQLERAGADVA"
BASE COUNT          465 a          769 c          797 g          409 t
ORIGIN      
        1 ccatcgtgtt ccagctcggt gagatcaagg atctgatcga cgagccgggc ctgtatttca
       61 agttcccgct gattcaaaac gtgcgctatt tcgacaagcg aatcctgacg ctggatacgg
      121 ccgacaccga gcgcttcatc acctcggaga agaaaaacgt cctggtcgac tccttcgtca
      181 agtggcgcat ccaggacatg cggcagtact acatcagcgt ccaaggggac gaacagcggg
      241 cccagctaca gctgagccag acggtaaagg ccgcgttgca ggacgaattc agcaagcgca
      301 ccgttcacga cgtgatttcc ggtgaacgcg acaagatcat gcaggtcgtt cgagaaaaag
      361 tcgacgaaga catgaagaag atcggcatgg agattgtcga cgtgcgcctg aagcgagtcg
      421 accttccgca ggaagtcagc gagtcggtct accgaaggat ggacgccgag cggaaaagcg
      481 tcgcagccga gcttcgctct cagggcttct ccgcggcgga gaaaatccgc gccgaagcgg
      541 accgcgagcg cgagatcatc attgccgggg cttaccgcga cgctcagcgc gtcaaaggcg
      601 agggcgacgc caaggcggcc tccgtctacg cgcgcgcgtt caacgagaat cccgagttct
      661 acgccttcta ccgcagcctc gacgcctacc gggcgagctt caagaacaag agcgatgtga
      721 tcgtgctcga ccccaattcc gagttcttca agtacttcag aaactctgga aagggcggca
      781 agtgaatggg cacgacgttt atcatggcat ttgccctcat gctggtcatc gagggcctgc
      841 tgccgttcct tctgccgacg ctgtggcgca ataccttccg ccgcatcacg gagttcactg
      901 acgggcagat ccggtttttc gggctctcct cgatgctggt cggcctgctg atcctgtttt
      961 tcatccgcta ggcgcgcttg ccccgaagga acctgatgcg caactggctc cttccggaaa
     1021 aattcgagga catccttccg ccgcaggcgc ggcgcatcga ccggatgcgg gcaaggctcc
     1081 tcgagctgtt ccgcgtgcac gggtacgagc tcgtgattcc ccccatgctc gagtacctcg
     1141 aatcgctgct gacgggcacc gggcacgatc tggatcttcg caccttcaag ctggtggatc
     1201 agctctccgg gaggatgatg ggggtgcgcg ccgacatcgc gccccaggtc gcgcggatcg
     1261 atgcgcacct tctgaacagg aagggcgtcg cgcggctgtg ctacgccggc agcgtactgc
     1321 tcacccggcc gcgtggcctc gactcgaccc gcgagcccct gcaggtcggc gcggaaatct
     1381 acggtcaccc cggaatcgag agcgatctcg agatccagca gcttctcgcc gagtcgctgg
     1441 cggcatgcgg catttccggg gcgcgactcg atatcggcca cgtcgccgtg ttccgcgcga
     1501 tttgccggca cggcggcgtc ggtgccgagc tcgagtcgga actgtacgaa gcgctggagg
     1561 gcaaggatct gccggcgctg cgcgcggtga ctcgatccat gccggaagcg acaagcaagg
     1621 cattgctcgc ccttccggag ctctatggcg acggcaccgt gctcgagcgc gcccggcgcg
     1681 tgctgcccaa gtatcccgaa atcgacgcgg cgctcgggga cctcgaggtt ctctcccgaa
     1741 gcggcagcat ccccgtgtcg gtcgatctgg cggaccttcg cggataccag taccacagcg
     1801 gagtggtgtt cgcggcctat gcgccgggcg tggccaacgc ggccgccctc ggcggacgat
     1861 acgacgaggt cggcaaggca ttcggccgcg cgcgcccggc aacaggattc agcatggacc
     1921 tgcgcgatct cgcgcgcact gcgccgcacg aagcggaagc ggccgcgatt cgagcgccgt
     1981 actcgagcga tgcggggctc gccaccgcga tccgcaagct gcgaatcgcc ggggaggtcg
     2041 tagtggtcgg gcttccggga cacgccgcgg aagacgctca gcccgactgc gaccgggagc
     2101 tcgtaaagca ggccggcgag tggaccgtgc gcaaactggg caagagctga gacggtggcc
     2161 aagaacgtcg tagtcatcgg aacccagtgg ggcgacgagg gcaagggcaa ggtggtggac
     2221 tggctcaccg accatgccca gggagtggtg cgcttccagg gcggacacaa cgccgggcac
     2281 acgctggtga tcggcggcaa gaagaccgtg ctgcacctcg ttccctcggg catcttgagg
     2341 aatggcgtga cctgctacat cggcaacggc gtcgtgctct ccccacaggc gctgatcgat
     2401 gaaatcgacc agctcgagcg tgccggcgcg gacgtcgccg
//
LOCUS       DASPZD010000397         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_640962, whole genome shotgun sequence.
ACCESSION   DASPZD010000397 DASPZD010000000
VERSION     DASPZD010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640962"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VEM33_07710"
     CDS             <1..606
                     /locus_tag="VEM33_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51751.1"
                     /translation="RYLSAYEVETRGVYIQDVTFPEELVSVLTRREIANQEKATYEQQ
                     KDAQTIRIDLEKARGTADMQAALAQAQVSVDINIAKANARKAEADGEAKYVELTGAAE
                     GSRRRAIGLGDAAATEALGLARAKGYDAQIRSLGSGPTAVVAVANAVAEGKLTIVPEV
                     LVTGGGGAVEGLAAALMRSLNGNGHAKRDDAKDKPPVSHRK"
     gene            1083..1877
                     /locus_tag="VEM33_07715"
     CDS             1083..1877
                     /locus_tag="VEM33_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51752.1"
                     /translation="MSRALLPDDLEAALDQLSQLPRKPAARTVLLERFGALTANRRRD
                     ADGLLRWLLLRALRDLVTGADVPLLLEATRTFEHVPPSFDEVAHGIRAEGLHRLLELD
                     GDLARWRAIEMLADGQDNPVTGEPARTALRVLGSAGELTLLYGIALDNPYGLPPAARA
                     ESLLWLDGLPDDRLRPVIDRFLEKDEPNLLLPVIELGIERRVGWLEDVLINALLATSH
                     VDAFRYAILQAIARHRLDLVERIRGRLDSKEQAEKIAVLRELRAGT"
     gene            2071..>2673
                     /locus_tag="VEM33_07720"
     CDS             2071..>2673
                     /locus_tag="VEM33_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51753.1"
                     /translation="MTDDDLPPERLASVFAHFVQQVASSAVAQASPLLDKIREQLGTD
                     PAELPVTLEQIDTFEHPNLQLAIDAYLRQDGHSADLFGVSMDNKRFMSVGLSELVIRE
                     GGIHRPPLLEGPVDYVNYHLAGDEVLACVQFGLYLVRAGDARLVTLVTGPSEMGDPRR
                     QKLRLEVLASRPADAVAFIADIRKLMDQVNVYRGHAITLSA"
BASE COUNT          537 a          830 c          842 g          464 t
ORIGIN      
        1 cgctatctct ccgcctacga ggtagagacg cgcggcgtgt acatccagga cgtgaccttc
       61 cccgaggagc tcgtcagcgt gctcacgcgc cgcgagatcg ccaaccaaga gaaggcgacc
      121 tacgagcagc agaaggacgc gcagacgatc aggatcgacc tcgagaaggc gcgaggtacg
      181 gcggacatgc aggccgcgct cgcccaggct caggtttcgg tcgacatcaa catcgccaag
      241 gccaacgcgc gcaaggcgga ggccgacggc gaggccaagt acgtcgagtt gaccggcgct
      301 gcagaagggt cacgccggcg ggccatcggg ctcggagatg cggctgcaac cgaagcgctc
      361 ggcctggcgc gcgcgaaggg ctacgacgcc cagattcgaa gtctgggttc cggaccgacg
      421 gctgtcgtgg cggtcgccaa cgcggtagca gaaggcaagt tgaccatcgt gcccgaagtg
      481 ctggtcactg gcggcggcgg agccgtggag ggactggccg cggcgctgat gcgctcattg
      541 aacggcaacg ggcatgccaa gagagatgac gccaaggaca aaccgccggt gtcccaccgc
      601 aagtaaacgg ataggcaatc gccatcagcg agccggaggg cacgggccct ccggtctcgt
      661 ttttttgata tcccgcacga acgctcaccg gcgaaaaatc aggccgtagg acggctcggc
      721 gaagcgatcc aagcagtatc gccgacaagg tccgcgaccc aacattcgag atggacgggc
      781 gacgattccg tcacggctga ggaccatttg gctcgcgcgc gcacgcgcgt catctctatg
      841 tatgagccag agatcgaggt cacgaggttg tacgcaccga gttgagctgc tcggttgcgc
      901 tcgataggac tttggtatca actgccttgt cgaatcagac caaaaggccc tatctcgcat
      961 tcggcgttcc cagaagcgct tgagctcttc aaagcgcggt gccggcgtca cgctagattg
     1021 cgaacacgga gcggaacaca gacacgaaag acgaagccga actacaacga gcccttgcgc
     1081 gagtgagtcg ggcgctgttg cctgacgatt tggaggctgc cctggatcag ctctcgcaat
     1141 tgcctcgtaa gcctgcggcc agaaccgttt tactggagcg tttcggtgcg ctgaccgcta
     1201 accggcggcg cgacgccgat ggcctccttc gctggttgct gcttcgcgcg cttcgtgacc
     1261 tcgtgacggg cgccgacgtg ccactcctgt tggaagcgac gcgaacgttc gagcacgtgc
     1321 cgccttcgtt cgacgaggtc gcacacggga ttcgggccga ggggctccat cgcctgctcg
     1381 aactggatgg tgaccttgcg cgctggcgag ccatcgagat gctcgcagac ggccaggaca
     1441 atccagtgac cggcgaaccg gcccggacag cgctgcgcgt tcttggctcc gccggagagt
     1501 tgacgctcct atacgggatc gcgctcgaca atccctatgg cctgcccccg gccgcccggg
     1561 ccgagtcact actttggctt gacggactgc cggacgaccg gctccggccg gtcatcgatc
     1621 gattcctcga gaaggacgag ccgaatctgc tccttccagt gatcgaactc gggatcgaac
     1681 gtcgagtcgg ctggctggag gacgttctga taaacgcact cctcgctacc tcgcacgtag
     1741 atgcgttccg ctacgcaatc ctccaggcga ttgctcgtca ccgcctagat ctggtcgagc
     1801 gaatccgtgg ccggcttgac tcaaaagaac aggccgaaaa gatcgccgta ctacgggagc
     1861 ttcgggctgg gacgtagggc ttgattcgct taggcggaca cataacgcaa tcacccacag
     1921 ggatcgggca agaacatcca ccagcatctc ggcgaggtct gacgctctgc cggggttaac
     1981 ggctaccgcg atccgtggca cccgactccg acaacctggc gctggagcag agcgggagtt
     2041 gcgcttacac aagcgttgga taatcggtcc atgactgacg atgaccttcc gcctgagcga
     2101 ttggcgtccg tcttcgccca ctttgtgcag caagtcgcgt cctcagccgt cgcacaggcc
     2161 agccccctcc tggacaagat ccgggagcag ctcggaaccg atccagccga gctcccagtg
     2221 acgcttgagc agatcgacac gttcgagcat cccaacctgc aattggcaat cgacgcctat
     2281 ctgcgacaag acggccattc ggccgacctg ttcggggtca gcatggacaa caagaggttc
     2341 atgtcagtcg gattgtcaga gttggtaatc cgcgagggcg gtatccatcg gcccccactc
     2401 ctcgaaggcc ctgtggatta cgtcaactac cacctggcag gcgatgaggt cctggcctgc
     2461 gttcaattcg gcctctacct ggtgagggct ggagatgcca gattagtgac gctggtgaca
     2521 ggcccgagcg agatgggcga ccctcgccgc caaaagctgc gattggaagt cttggcatcg
     2581 cggccagccg atgcggtggc attcatagcc gacattcgaa agctcatgga tcaggtcaat
     2641 gtgtatcgcg gccacgccat cacgctgtcg gcc
//
LOCUS       DASPZD010000398         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_159288, whole genome shotgun sequence.
ACCESSION   DASPZD010000398 DASPZD010000000
VERSION     DASPZD010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159288"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VEM33_07725"
     CDS             <1..225
                     /locus_tag="VEM33_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51754.1"
                     /translation="SWVGAIAPSGTPRAAIEKLNGALAKVLSSPDVKSRFDTQSAEIV
                     ASTPEQFGELIKSETVKWGRIIREKNIRVD"
     gene            complement(246..1064)
                     /locus_tag="VEM33_07730"
     CDS             complement(246..1064)
                     /locus_tag="VEM33_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51755.1"
                     /translation="MRSATENLVEISGVEFSYGARKLLKGLSLRIPRGKLVAILGASG
                     SGKSTLLRLIGGQATPDRGTVKVAGQVVHELDTHALYELRRKMGMMFQAGGLFTDMSV
                     FENIAFPMREHTELPESVLRRIVLMKLHAVGLRGTQALTPSELSGGMARRVALARAIA
                     MDPMLAMYDEPFGGLDPISINVIANLIRQLNDSLGATSIVVSYDVSEAVKLVDYVYVI
                     ADGVVAGEGTPEEMLASKDPYVHQFMHAQPDGPIPFHYPGRPIGEDLGLRGAAR"
     gene            complement(1073..2347)
                     /locus_tag="VEM33_07735"
     CDS             complement(1073..2347)
                     /locus_tag="VEM33_07735"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYS51756.1"
                     /translation="MNAHEIWLAAGLRTPFARVDGPLARLDAVELSLPVVRAMTGSQS
                     AAKPDLVVWGTVIPNLGYSNIAREVQIEAGLDQTIPAFSTVLACSTSMVAAFEAAGML
                     GHGGKELALVGGVESMSRVQIGLSQNFSDWLRRLSQARSIVQRLALFKNLRPRDIRLH
                     ILAVANRATGKSMGEHCEEMARTWSIARDSQDKIALASHEKALAGQKSGFFDDLIVPA
                     EGMSRDGFPRADTSLERLAKLQPAFDRKSGKGTITAGNASPLTDGAAGLWVATGAGLK
                     RLPASVPRARLVDWEIAAVNVFTEGLLMAPAYAIPRMLARHSLAYGDIALWEIHEAFA
                     AQVLCNVAALESASFRREKAGVEVNPGRFPWERLNPHGGSVALGHPFGATGARILSQA
                     VKELAAMPGGSRAVVSICADGGLGTVALLQAQ"
     gene            2386..3030
                     /gene="msrB"
                     /locus_tag="VEM33_07740"
     CDS             2386..3030
                     /gene="msrB"
                     /locus_tag="VEM33_07740"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYS51757.1"
                     /translation="MQTPAGIPGPEGGLYCGLAEVMKMDRRYWLRGLTALSLAGFGAA
                     AWGQKPAKKGAVKVEDLQKNWKALLAEGADVATSADPLKLPDAEWKKRLAPPAYDVLR
                     HEGTERAGTSPLDREKRPGVFVCAGCSLPLFTSAMKFDSGTGWPSFFTSIPGVFGTTT
                     DRKLFMERTEYHCVRCGGHHGHIFDDGPDPTGLRYCNNGVALRFIPKGPAQKPA"
BASE COUNT          566 a         1061 c         1055 g          524 t
ORIGIN      
        1 tcctgggtgg gagccatcgc cccctcgggc acgccgcgcg cggccatcga aaaactcaac
       61 ggcgcgctcg cgaaagtcct gtcttcgccc gacgtgaaat cgaggttcga cacgcaaagc
      121 gccgagatcg tcgcgagcac accggagcag ttcggagagc tgatcaagtc cgagacggtg
      181 aagtggggca ggatcatccg cgagaagaac atccgggtcg actgacgccg cagccccgca
      241 ttgcattatc gggccgcgcc tcgcaatccg agatcctcgc cgatcggccg cccgggataa
      301 tggaagggga tcggcccgtc gggttgcgcg tgcatgaact ggtgaacgta gggatccttg
      361 gaagcgagca tctcctcggg cgttccctcg cccgcgacca cgccgtcggc gatcacgtac
      421 acgtagtcga cgagcttcac cgcctcgctc acgtcgtagc tcaccacgat ggacgtcgcg
      481 cccagcgaat cgttcagctg ccggatgagg ttcgcgatca cgttgatcga aatgggatcg
      541 agccccccga agggctcgtc gtacatcgcg agcatcggat ccatcgcgat cgcacgcgcg
      601 agcgccacgc gccgcgccat ccccccggag agctcgctcg gcgtcagcgc ctgcgtgccg
      661 cgcaatccga ccgcgtgcag cttcatcagc acgatcctgc gcagcacgct ctcggggagc
      721 tcggtgtgct cgcgcatcgg gaaagcgatg ttctcgaaca ccgacatgtc ggtgaaaagc
      781 cctcctgcct ggaacatcat ccccatcttg cggcgcagct cgtacagggc gtgcgtgtcg
      841 agctcatgaa ccacctggcc ggcaaccttg accgtgccgc ggtccggcgt cgcctggccg
      901 ccgatcaggc ggagcagcgt cgacttcccc gagccgctgg caccgaggat cgcgacgagc
      961 ttgccgcgcg ggatcctgag gctcaggccc ttcaggagct tcctggcgcc gtaagagaat
     1021 tcgacgccgg agatctcgac gagattttca gtggcggatc tcatttctgc cgtcattgtg
     1081 cctggagcag agccacggtg cccagtcctc cgtccgcgca gatgctcacc accgcgcggc
     1141 ttcctccggg catggcggcg agctccttca ccgcctgcga aaggatgcgc gcgccggtcg
     1201 cgccgaatgg gtggccgagc gccaccgagc cgccgtgggg attgagccgc tcccagggaa
     1261 atctgccggg gttcacctcg acgcccgctt tctcgcgccg gaagctcgcg ctctccagcg
     1321 cggcgacgtt gcatagcact tgcgctgcga acgcctcgtg gatctcccac agcgcgatgt
     1381 cgccgtaggc gaggctgtgg cgcgcgagca tgcgcgggat cgcatacgcg ggtgccatga
     1441 gcaatccctc ggtgaagacg ttgactgccg cgatctccca atcgacgagg cgcgcgcgcg
     1501 gtacggaggc gggcaggcgc ttgagtcccg cgccggtggc cacccacagg cccgccgcgc
     1561 cgtcggtgag cggcgaggcg tttccggcgg tgatcgtgcc cttgccgctc tttctgtcga
     1621 aggccggctg cagcttagcc aggcgctcga ggctcgtgtc agcgcgcgga aaaccgtcgc
     1681 gcgacatgcc ttcggcagga acgatcaggt cgtcgaaaaa tccggatttc tgaccagcca
     1741 gcgccttctc gtggctggcc agcgcgatct tgtcctggga atcgcgcgca atgctccagg
     1801 tcctggccat ttcctcgcag tgctcgccca tgctcttgcc ggtggcgcgg ttggccacgg
     1861 caagaatgtg caggcggatg tcgcgcggcc ggaggttctt gaacagcgca aggcgctgca
     1921 ctatcgagcg cgcctgcgag aggcgcctca gccagtcgga gaaattctgc gaaagcccga
     1981 tctgcacgcg gctcatgctc tccacgcccc cgacgagggc gagctccttg ccgccgtggc
     2041 cgagcattcc cgccgcctcg aaagccgcga ccatgctcgt cgagcaggcg agcaccgtgg
     2101 aaaacgccgg gatggtttga tccaggcccg cctcgatctg cacctcgcgc gcgatgttcg
     2161 agtagccgag gttgggaatc accgttcccc agacgacgag gtccggcttc gcggcggact
     2221 gcgatccggt catcgcccgg acgacaggca gcgaaagctc gaccgcatcg aggcgcgcga
     2281 gcgggccgtc cacgcgggcg aacggtgtgc gcaggccggc cgcgagccag atctcgtgag
     2341 cgttcatgat gcctcattgt aaggccggcc cggttcttac gactcatgca gacgccggcg
     2401 ggaattcccg gcccggaagg cggtctatac tgcgggcttg cggaggtgat gaaaatggat
     2461 cgtcgctact ggttgagagg cttgacggca ctgtctctgg cgggtttcgg tgccgcagca
     2521 tggggacaga agcccgcgaa aaaaggagcc gtgaaagtgg aagacctgca aaagaactgg
     2581 aaagccctgt tggccgaagg ggcggacgtc gccacgagcg ccgatccgct caagctcccg
     2641 gacgccgaat ggaagaagcg gctggcgccc ccggcctacg acgtgctgcg ccatgaaggc
     2701 accgaacggg ccgggacgag cccgctcgac cgtgaaaagc gcccgggggt gttcgtgtgc
     2761 gcgggctgca gcctcccgct tttcacctcg gcgatgaaat tcgacagcgg caccggatgg
     2821 ccgagttttt tcacttcgat acccggcgta ttcgggacca cgaccgaccg caagctcttc
     2881 atggagcgca ccgaatacca ctgcgtgcgc tgcggcgggc atcacggcca catcttcgac
     2941 gatgggcccg acccgaccgg cctgcgctac tgcaacaacg gcgtcgcgct gcgattcata
     3001 cccaaggggc cggcacagaa acccgcctaa aacgccttcg cgatagggtt cctatcgctt
     3061 ccgtgcctct acatgtcgac ccggagggtg cggtgcgcct tccatgcgcc gtagatcaac
     3121 agaagggtaa ggagtaccgc tgtgattgcg aacaccgagt aagccatgtc cgacggggac
     3181 aaggatatgc cggtcgcggg gacggg
//
LOCUS       DASPZD010000399         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1366074, whole genome shotgun sequence.
ACCESSION   DASPZD010000399 DASPZD010000000
VERSION     DASPZD010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366074"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VEM33_07745"
     CDS             <1..544
                     /locus_tag="VEM33_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51758.1"
                     /translation="GTPPVAKSAGPSSYSYGEAERFHTFQPSAEGDAREQGLETWETL
                     GESDDGFRLRRGTQGMRLAHRQLVALRPSGARQFILCHVDWLIEGPDQTLTIVARALK
                     GEAKACAVRSSDRGAPQRSDALMLPVAPGLSPALVLPTGWYRRAGELELELGSATKRV
                     TLSGVLERGFDYERVRVSTS"
     gene            620..988
                     /locus_tag="VEM33_07750"
     CDS             620..988
                     /locus_tag="VEM33_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HYS51759.1"
                     /translation="MTTADRPTPTEIRLHQKSRVLEVSFSDGSRFELPYEFLRVYSPS
                     AEVRGHGPGQEVLQTGKKDVDILRLEPVGSYAVQPHFSDGHGTGIYSWDYLYDLGSNR
                     QSLWRTYLTRLEAAGAHREP"
     gene            1033..1764
                     /gene="ubiE"
                     /locus_tag="VEM33_07755"
     CDS             1033..1764
                     /gene="ubiE"
                     /locus_tag="VEM33_07755"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HYS51760.1"
                     /translation="MSQTQFGYQRVDEAEKASRVAGVFSSVASRYDLMNDLMSGGVHR
                     LWKAFAVALAGVREGERVLDVAAGSGDLALDFAKRTGASGEVWLTDINRAMLERGRDR
                     LLDRGRPVPAVQCDAEGLPFPSNYFDCVSVAFGLRNMTRKDAALDEMRRVLRPGGRLL
                     VLEFSRVWRPLAKAYDWYSFKVLPWLGKTVTGDEAAYRYLAESIRMHPDQESLKGMLE
                     RAGLENVEYFNLSAGVVALHRGYKF"
     gene            1781..2626
                     /locus_tag="VEM33_07760"
     CDS             1781..2626
                     /locus_tag="VEM33_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HYS51761.1"
                     /translation="MKKIIISLFTLFLGAGIFVDDVEAARLGGGRNLGAQRQVTAAPK
                     QATPPAQQQQPATAPQSSGPGRWFAPLAGLAAGLGLGWLFAQGGFGAAIGALLLALFA
                     GVAVFALMRLLSKRRPQRAQMQYAGFGNETVAAPPPSQLPGDAGAQPNYRSQFVSNIP
                     AGFDVEAFLSEARRNFLRLQQANDRGDLARLRQVTTEDMFNTLKNDVAGHSGAQQTDV
                     VTLNAALLEVVTEGELHWASVRFSGSIREEASAPTEAFEEIWHMRKPLNGSSGWLLAG
                     IQQPS"
     gene            2663..3250
                     /locus_tag="VEM33_07765"
     CDS             2663..3250
                     /locus_tag="VEM33_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol-binding protein"
                     /protein_id="HYS51762.1"
                     /translation="MIERSALAALNHLLEQQPWAAERLRAFAGQAVEFRCPPFPDLRL
                     RITGNGLLERAQAETAGSLVVKLNPGTLPLLLARDETARKHVEIEGSADLAGTVDYLF
                     SHLIWDFEEDLSRVFGDVVAHRLASGGKAVAAWQRDAALRLAESLAEYWTEEQPLVAR
                     PADVGSFCRNVDTLRDDVARLEKRIEQLSDPRNER"
     gene            3326..>4359
                     /locus_tag="VEM33_07770"
     CDS             3326..>4359
                     /locus_tag="VEM33_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HYS51763.1"
                     /translation="MFRLAKILWITLRFGLDEFALASGSRLARALNALLFFRSLSEPR
                     GARLRLALEALGPIFVKFGQVLSTRRDLLPADLADELAKLQDRVPPFPGELAVAEVER
                     ALGKPIAKIFEKFELQPVASASVAQVHLAVLPGGTEAAVKILRPGMMPVIAKDLALMD
                     AGAGLVERFWSDGKRMRPREVVAEFARHLAEELDLVREAARASQLRRNFPDSPLLAVP
                     EVYWDFCTGTVMTMQRMHGTPVSRVDELRQQGVDIPKLARTGVEIFFTQVFRDGFFHA
                     DMHPGNILVTPEGRYVALDFGIMGTLSEVDKQYLAQNFLGFFRRDYARVARAHLEAGW
                     VPADTRMDEF"
BASE COUNT          719 a         1438 c         1445 g          757 t
ORIGIN      
        1 aggcaccccc cctgtagcga agagcgcggg tccttcgagc tattcctacg gggaagccga
       61 gcgatttcat acgttccagc cgagcgcgga aggggatgcc cgcgagcagg gtctggagac
      121 ctgggaaacg ctcggagaat cggacgacgg gttcaggctt cgtcgcggga cccagggcat
      181 gcgtctcgcc caccgacagc tcgtggcgct gcggccaagc ggcgcgcgcc agttcatcct
      241 ttgccatgtc gactggctca tcgaaggacc ggatcaaacg ctcaccatag tcgcccgagc
      301 gctgaaaggg gaagcaaagg cctgcgccgt gcgctcgtcc gaccggggcg ccccgcagcg
      361 gtccgatgcg ctgatgctgc cggtcgcccc gggcctgtcg cctgcgctgg tgctgcccac
      421 gggttggtac cggcgcgccg gcgagctcga actcgagctc ggcagcgcca caaagcgcgt
      481 cacgctctcc ggagtcctcg agcgcggttt cgactacgag cgggtccgcg tttcaacgtc
      541 ctgaataggc gcaagtctcg tctctccgga gcacgcacaa gcaggagttc ttcgcgcggc
      601 cggtcgtcta aaatgcctta tgacgaccgc ggacagaccg acacccaccg aaatcaggct
      661 gcaccagaaa tcccgggtgc tcgaggtttc gttctccgac ggcagtcgct tcgagctgcc
      721 ctacgaattc ctgcgcgtgt actccccgtc cgccgaagtg cgcggccacg gaccgggaca
      781 ggaagtcctg cagaccggca agaaggacgt tgacatcctg aggctggagc cggtgggcag
      841 ctacgcggtg cagccgcatt tttccgacgg gcacggcacc ggcatttact cctgggacta
      901 cctctacgat ctgggctcga atcgtcaatc tctctggcgg acctatctca cgcggctcga
      961 ggcagccggg gcgcaccgtg agccctgagc ctccggcatg aacacgtctt gacggcgtgc
     1021 tagcatgctc gcgtgagtca gacccagttc ggataccagc gcgtcgacga ggccgaaaag
     1081 gcttcgcgcg tcgccggcgt tttttcctcg gtcgcatccc ggtacgatct gatgaacgat
     1141 ttgatgtcgg gcggcgtgca ccggctgtgg aaggctttcg cggtggcgct cgccggcgtg
     1201 cgcgagggcg agagggtgct cgacgtggct gccggatcgg gcgatctggc actcgatttc
     1261 gcgaaacgta ccggggcgag cggagaggtg tggctcaccg acatcaaccg ggcgatgctg
     1321 gaacgggggc gcgatcgcct gctcgaccgc ggccgcccgg tcccggcggt gcagtgcgac
     1381 gcggaggggc tgccgtttcc ttcgaactac ttcgactgtg tcagcgtcgc cttcggtctg
     1441 cgcaacatga cgcgaaagga cgcggcgctc gacgaaatgc gccgcgtgct gaggcccggg
     1501 gggagactgc tcgtcctcga gttctcgcgc gtctggcggc cgctcgcaaa ggcctacgat
     1561 tggtactcgt tcaaggtgct tccctggctg ggcaagacag tcacgggcga cgaggccgcc
     1621 taccgttatc tcgccgaatc gatacgcatg caccccgacc aggagagcct gaagggcatg
     1681 ctggaacggg cggggctgga gaatgtcgaa tatttcaacc tgagcgcggg tgtcgtggcc
     1741 ctgcatcggg gctacaaatt ctgaatcgcg gaaagagacc atgaaaaaaa ttatcatttc
     1801 actgttcacg ctgttcttgg gtgcaggcat cttcgtggat gacgtcgagg ccgcaaggct
     1861 cggcggcggc cgaaatctcg gggcccagcg ccaagtcacg gcggccccga aacaggccac
     1921 tccgcctgcg caacagcagc aaccggcgac ggcgccgcaa tcgtctgggc cggggcgctg
     1981 gtttgcgcct ctcgcgggac tcgccgcggg ccttggcctg ggctggctgt tcgcgcaggg
     2041 aggcttcggc gccgcaatcg gcgcgctcct cctggctttg ttcgccggtg tcgcagtgtt
     2101 cgccttgatg cgtctcctgt cgaagcggcg cccgcagcga gcgcagatgc agtacgccgg
     2161 cttcggcaat gaaaccgtgg ctgcgccgcc gccctcgcag cttcccggag acgcgggcgc
     2221 gcagccgaat tatcgcagcc agttcgtttc caacattccg gcgggcttcg acgtcgaggc
     2281 ctttttgagt gaggcacggc gcaacttcct gcgcctgcag caggcgaacg accgcggcga
     2341 cctcgcgcgg ctgcgccagg tcaccacgga ggacatgttc aacaccctga agaacgacgt
     2401 cgccgggcat tcgggcgcgc aacagaccga cgtcgtcacc ctcaacgcgg cgctcttgga
     2461 ggtcgtcacc gagggcgagc tgcattgggc gagcgtgcga ttttccggtt cgattcgcga
     2521 agaggcgagc gcaccgaccg aggcattcga ggagatctgg cacatgcgca agccattgaa
     2581 cggttcctca ggctggctgc tcgccgggat acaacagccc agctgatccc cgctcgccac
     2641 ggggcccgac ccggataccc ccatgatcga gcgcagcgcc ctcgcagccc tgaaccatct
     2701 tctggagcag cagccgtggg cggccgagcg cctgagagcc tttgcgggcc aagccgtgga
     2761 atttcgctgc ccgccttttc cagacctgcg cctgaggatc accggcaacg gccttctcga
     2821 gcgggcgcag gcagaaaccg cgggctcgct cgtcgtgaag ctcaacccgg gcaccctgcc
     2881 gctcctgctt gcacgcgacg aaaccgcgcg aaagcacgtt gaaatcgaag gctcggcgga
     2941 tctggcgggc acggtcgact atcttttcag ccacctcatc tgggatttcg aggaagatct
     3001 ctccagggtg ttcggcgacg tcgttgccca ccgcctcgcc tccggcggca aggccgtcgc
     3061 ggcgtggcag cgcgacgccg cattgcgcct cgccgaaagc ctcgccgaat actggaccga
     3121 ggaacagccc ctggtcgcgc ggcccgccga cgtggggagc ttttgccgca acgtcgacac
     3181 gctgcgcgac gacgtggcgc ggctggagaa acgcatcgag caactgtcgg atccccgcaa
     3241 cgagcgctga gggcgcggcc ccttttcggg tcgctacggc caattctggt aaattggccg
     3301 cttcgccccg cggcctcgcc ctgccttgtt tcgcctcgcc aagattctct ggataaccct
     3361 gcgcttcggg ctggacgagt tcgcgctcgc ctcagggagc aggctagcgc gcgccctgaa
     3421 cgcgctcctt ttctttcgct cgctctccga gcctcgcggc gcgcggctgc gcttggcgct
     3481 cgaagcgctg ggccccatct tcgtcaagtt cggccaggtg ctctcgacac gccgcgacct
     3541 tttgcccgcc gacctcgccg acgagctggc gaagctccag gacagggtgc ccccttttcc
     3601 cggcgagctc gcggtcgctg aggtcgagcg cgccctcggc aagccgatcg cgaaaatctt
     3661 cgagaaattc gagctgcaac ctgtcgcgag cgcgtcggtc gcccaagtgc atctcgccgt
     3721 gctccccggg ggcacggagg cggcggtcaa gatcctgcgg cccgggatga tgccggtgat
     3781 cgcaaaggac ctggcgctca tggatgccgg tgcgggcctc gtcgagcgct tctggtccga
     3841 cggcaagcgc atgaggccgc gcgaagtggt cgccgaattc gcgcgccatt tggccgagga
     3901 gctcgacctc gtgcgtgaag ccgcgcgtgc aagccagctg cgccgcaatt tccccgactc
     3961 gccgctgctc gccgtgccgg aggtgtactg ggacttctgc acgggcactg tcatgacgat
     4021 gcagaggatg cacggcaccc cggtgagccg ggtagacgag ctgcgccaac agggcgtgga
     4081 cattcctaag ctcgcgcgca cgggcgtcga gatctttttc actcaagtgt tccgagacgg
     4141 cttttttcac gcggacatgc atccgggaaa catcctggtg acgcccgagg gccgctacgt
     4201 cgcgctcgac ttcggcatca tggggactct cagcgaggtg gacaagcagt atctcgcgca
     4261 gaacttcctc ggttttttcc ggcgcgatta cgcccgcgtg gcgcgagcgc atctcgaggc
     4321 gggctgggtg ccggccgata cgcgaatgga cgagttcga
//
LOCUS       DASPZD010000400         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1486170, whole genome shotgun sequence.
ACCESSION   DASPZD010000400 DASPZD010000000
VERSION     DASPZD010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486170"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VEM33_07775"
     CDS             <1..270
                     /locus_tag="VEM33_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51764.1"
                     /translation="DCIVRFLTKGSPRKAAEIAAFYADAAPVARRLDWAQSDSLERLG
                     IEADFIDAGLLLEGIGPVREAMDAALDQLNEIRAELWTAAVAARP"
     gene            458..1252
                     /locus_tag="VEM33_07780"
     CDS             458..1252
                     /locus_tag="VEM33_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYS51765.1"
                     /translation="MPASPDVVARYLAAHATTHVVATLARRLVAIRRAHALRGLPDPV
                     RSELVRLTFRGIRRAHGRPQRRAAALATEEMAAIVASLGDSVRDARDRALLLIGFAGA
                     FRRSELICIDCKSIERRAAGIVITIPKSKTDQEGQGRQVAIPHGRNDICPVRALDQWL
                     ELSGITQGPVFRPLARRGHVLPGQLSGDSIASIVKQRVKAIGLDPARYSGRSLRAGFV
                     TSAATAGAPTWRIKAQTGHASDALVGRYIRLSDPFAATAGSWIAAF"
     gene            1792..>2133
                     /locus_tag="VEM33_07785"
     CDS             1792..>2133
                     /locus_tag="VEM33_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51766.1"
                     /translation="MDIETARAAVAAGDAKYGRVFQELCDWQYRAFERLRDDGAISKK
                     GFDRMVAVPHGRIVVDGARGGPWIGARTLTDGEVIDSGAVACFVFHESINTQPLMMGI
                     VFYPFVSPGVSD"
BASE COUNT          373 a          668 c          677 g          415 t
ORIGIN      
        1 gactgcatcg ttcggttcct gacgaagggc tcgccgcgga aggctgccga gatcgcggcg
       61 ttctacgccg acgccgctcc ggtggcgcga cggctcgatt gggcgcaatc ggattcgttg
      121 gagaggctgg ggatcgaggc ggatttcatc gatgcggggc tcctgctcga ggggattggc
      181 ccagttcgcg aggccatgga tgccgcgctg gaccagctca acgagatccg ggccgagctc
      241 tggaccgcgg ccgtcgccgc gcgcccctga cgctcatggc cattaccccc gataatcttc
      301 ccttatcaga accaaccccg cgcgccgcag tcaccgccac cccagccacc ccgattggga
      361 tcgcctgcac cgatccgagg gtccttcatt tcatcgaggc agccacagcg ccgaacaccc
      421 ggcgcgccta cgacagcgat ctcaagcatt tcctcgcttg ccggcgagcc cggatgtcgt
      481 cgctcgctat ctggcagccc acgccacgac ccatgtcgtg gccaccctcg cccgccgcct
      541 ggtcgcaatc cgccgcgcgc acgccctccg tggcttgcct gacccggtcc gatccgagct
      601 cgtccggctg accttccgcg gcatccggcg cgcccacgga cgcccgcagc ggcgcgcagc
      661 agccctcgca acggaagaga tggccgccat cgtggcttcg ctcggcgact ccgtccggga
      721 tgcccgggat cgcgccctgc tgctcatcgg gttcgccggg gcgttccggc gctcggaact
      781 catctgcatc gactgcaagt cgatcgagcg gagggctgct ggcatagtca tcaccattcc
      841 gaagagcaag accgaccagg aaggccaggg tcggcaggtc gctattcccc acggccgaaa
      901 cgatatctgt ccggtacggg cgctcgacca atggctcgag ctgtccggga ttacccaagg
      961 gccggtgttc cgaccgcttg cgcgacgcgg gcatgtgctg cccggtcaac tttcgggcga
     1021 ctctatagcg tccatcgtga agcagcgggt taaggcgatc ggcctcgatc cggcacggta
     1081 ctccggacgc tcgctgcgcg ccgggttcgt caccagcgcc gcgacggcgg gcgcgccgac
     1141 ctggaggatc aaggcgcaga ccggtcacgc ctcggatgcg ctcgtcggca ggtacattcg
     1201 tttgagcgat ccctttgccg ccactgcagg gtcgtggatt gcggccttct gattgtgtgg
     1261 taatcaaaaa aggatgcaca tgggcgattg ggaagtcatt cttcagcttg gggccgaagg
     1321 tggctcggtt acgctatatg ggatggaaac cgagcgtggg tggctctttt accgtgacgt
     1381 gagcgactgg tctccagagc tgataggcga agagcggatt caacataagt cacctgtcgt
     1441 gaactcttgg gaggccgctg ttgcccttct cgaccggtac ccgtggcaaa gactttgtcc
     1501 tttgatgatt cacccggaat tttcggagcg agttcggtct gcggcacgag atcgacttca
     1561 aagggatcaa gctcctgccc ggtactttga tatgtggtgc aggatgatca gcaccggaaa
     1621 taattccgtt taacgcccgg actcaagtac ccagcctgtg ttattcaacc ggtagctggg
     1681 gtaatcgaac tgagagcgcg tccgcgattt cggggcgtgc ccacagcttt ggcgatgccg
     1741 tccagcgcct ccaggtctgg acctatgact ggattgagag gactcctgcc gatggacatc
     1801 gaaacggcta gagctgcggt tgctgcaggg gatgcgaaat acgggagggt gttccaggag
     1861 ctgtgtgatt ggcaataccg cgctttcgaa aggcttcggg acgacggcgc catctcaaaa
     1921 aagggctttg ataggatggt ggccgtccca cacggccgca tcgttgtgga tggcgccagg
     1981 ggtggcccat ggatcggggc gcgtaccctg acggacgggg aagtgatcga cagcggagcg
     2041 gttgcatgct ttgtatttca cgaatccatc aacacccaac cgctcatgat gggcatcgtg
     2101 ttctatccct ttgtttcccc cggcgtctcc gac
//
LOCUS       DASPZD010000401         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_646946, whole genome shotgun sequence.
ACCESSION   DASPZD010000401 DASPZD010000000
VERSION     DASPZD010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646946"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..619)
                     /locus_tag="VEM33_07790"
     CDS             complement(206..619)
                     /locus_tag="VEM33_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51767.1"
                     /translation="MSDSDDLLGKADAFLKRYHPSGAAAQGEVPVLTEVVAEPAAAAA
                     RPSSGTPAQVGSIGSELPELEQRLKQSILDAIGPHIAKFVEEPLRARLDAHLQRTLTA
                     LSAQVKTDIETLIRDAVARAVELEIARARGQSRGG"
     gene            complement(616..1008)
                     /locus_tag="VEM33_07795"
     CDS             complement(616..1008)
                     /locus_tag="VEM33_07795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HYS51768.1"
                     /translation="MTRIDFYSNAEPKLQIACQLVVKAIQQQSRVVVYAPDENTARAF
                     DKLLWAHRAPSPEALVVIARRDSEMAHYQVILNLHTDSPPSFSRFERLVELVGAGDDD
                     RKLARSRFRFYRDRGYEINHYDLSKSSA"
     gene            complement(1021..>2357)
                     /locus_tag="VEM33_07800"
     CDS             complement(1021..>2357)
                     /locus_tag="VEM33_07800"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYS51769.1"
                     /translation="MDGKMGKTRLLYRVRGVAAERVLLVGLGQEKEFGEKQYRECARA
                     ALAAILETGARDACLYLAELQVPGRDAAWKARQLVLASAEVSYRFDGMKSKKAEERSL
                     ADVAISAVSKRDAAALERGVREGRAIAAGMTLTRDLGNLPANVCTPTYLAETSVKLGR
                     EWKLAVEVLEQKDMEKLGMGSLLSVTKGSHQPPKFVILRYSGAGKKDRPVVLVGKGIT
                     FDTGGISIKPSADMDEMKFDMCGAASVLGTLRAAAELGLKLNVIGLIPTCENMPGGAA
                     TKPGDIVTSMSGQTIEILNTDAEGRLILADALSYAERFEPQAVVDVATLTGACIIALG
                     HVCSGLFANEEGLAGELQAAGADSWDRVWRLPLWEDYQEQLKSNFADFANIGGRAAGS
                     VTAACFLSRFTRKYDWAHLDIAGTAWKSGKEKGATGRPVPLLMTFLIERAEK"
BASE COUNT          410 a          764 c          731 g          452 t
ORIGIN      
        1 actggatgca gtagggcggc ttttccgagg tcgcggaatg tctgaaatag ccgcgcgact
       61 cccaaaggga ataccagcgt cgctcgacct cgtgcgggtc aaagctcttg gcgagttcca
      121 tggcggtcga gttcaaaaag agagaacgcg ccgagcggcg cccccgcggg agcccggctt
      181 cgacgcgctc cggtggatta tagcgtcagc cgccccgcga ctgtccgcgt gcccgtgcga
      241 tttcgagctc caccgcacgg gcgaccgcgt cgcgaatcag cgtctcgatg tcggtcttga
      301 cttgggcgct gagggcggtc agcgtgcgct gcaagtgggc gtcgagccgt gcccgcaagg
      361 gttcctcgac gaacttcgcg atgtgcgggc cgatcgcgtc gaggatgctt tgcttcagcc
      421 tttgctccag ctccggcaac tcgctcccga tcgaaccgac ttgggccggc gtgcccgatg
      481 agggtcgcgc cgccgccgcg gccggttcgg cgacgacttc cgtaagaacc ggaacctcgc
      541 cctgagctgc cgcgccggaa ggatggtagc gcttcagaaa cgcatcggct tttcccaaca
      601 ggtcgtcgga atcgctcatg cgctcgactt ggagagatcg tagtggttga tttcgtagcc
      661 gcggtcccga tagaaacgaa agcggcttcg cgcgagctta cgatcgtcgt cgcctgcccc
      721 gacgagttcg accagccgtt caaagcggct gaacgacggc ggtgaatcgg tgtgcaaatt
      781 gaggatgacc tgataatgcg ccatctcgga gtcccggcgt gctatcacga cgagcgcctc
      841 gggagagggc gcgcggtgag cccaaagcag cttgtcgaag gcgcgggccg tgttctcatc
      901 aggggcatag acgaccacgc gtgactgctg ctggatcgcc ttgacgacaa gctggcaggc
      961 gatctgcagc ttgggttcgg cgtttgagta gaagtcgatg cgagtcatgg cgactcggtc
     1021 ttatttttcc gcgcgctcga tgaggaacgt catgagcagc gggacagggc ggccggtcgc
     1081 tcccttttcc ttgcctgatt tccacgccgt cccggcaatg tcgaggtggg cccagtcgta
     1141 cttgcgcgtg aatctcgaaa gaaaacaggc cgcggtaacg ctgcccgcag cgcgtccacc
     1201 gatgttcgca aaatctgcga aattgctttt cagctgttcc tgatagtcct cccacagcgg
     1261 caggcgccag acgcggtccc acgaatccgc tcccgccgct tgcagctcgc ccgcgagacc
     1321 ctcctcgttc gcgaacaacc ccgagcagac gtggcccagg gcgatgatgc aggcgcccgt
     1381 aagcgtggcg acgtccacga cggcctgggg ctcgaagcgc tcggcgtagc tcagtgcgtc
     1441 ggcaagaatg aggcgtccct ctgcgtcggt gttgaggatc tcgatggtct ggccggacat
     1501 gctcgtgacg atgtcgcccg gcttggtcgc cgcccctccc ggcatgtttt cgcaggtggg
     1561 gatcagaccg atcacgttca gtttcagccc gagctccgcc gcggcgcgca aggtccccag
     1621 cacgctcgcc gccccgcaca tgtcgaactt catctcatcc atgtcggcag aaggtttgat
     1681 ggaaatgccg ccggtatcga atgtaatgcc ctttcccacc agcaccacgg ggcgatcctt
     1741 cttgccggct ccggagtagc gcaggatcac gaacttgggc ggctgatgcg atcccttcgt
     1801 taccgagagc agcgaaccca tcccgagttt ttccatgtcc ttttgctcga gcacttccac
     1861 cgccagcttc cattcgcgcc cgagcttgac cgaagtctcc gccagatacg tcggcgtaca
     1921 gacgttcgca ggcagattgc ccagatcccg cgtgagcgtc atgccggcgg cgatggcgcg
     1981 cccttcgcgt acgccgcgct ccagtgcagc ggcgtcgcgc ttggaaacag ccgaaatggc
     2041 gacatctgca agcgaccgtt cttccgcttt tttgctcttc atgccatcga agcggtagga
     2101 aacctcagca gaggcgagga cgagctgacg cgccttccat gcagcatcgc ggccgggtac
     2161 ctgcaattcg gcgagataca ggcaagcatc gcgtgccccc gtttccagaa tggcggcaag
     2221 tgccgcgcgc gcgcattcgc gatactgctt ctcgccgaat tccttctcct gccccagccc
     2281 cacgagcagc acacgctctg ccgcgacgcc gcgtacgcgg tacaacagcc gggtctttcc
     2341 catcttgccg tccatgt
//
LOCUS       DASPZD010000402         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_29430, whole genome shotgun sequence.
ACCESSION   DASPZD010000402 DASPZD010000000
VERSION     DASPZD010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29430"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VEM33_07805"
     CDS             complement(<1..2078)
                     /locus_tag="VEM33_07805"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HYS51770.1"
                     /translation="MVSLFIAILALPMLSGKWLASPDGDQYSSGYAVKEWAANEYHRT
                     GHFPLWNPMIMGGLPHIDVVTHGDVLYPTSFLREVMPPYQAMNIAFVLHYILAGFLTY
                     LFLRRLGASWIGAVTGGVAYQLSGILISYVHPGHDGKLFVSTMLPLAFLALLAALRDK
                     RAWGYPLLAVAVALCLLSPHVQTTYYLLIAAALFALYLTFGEPSPDPLRSRLIRLGIV
                     FVAVVVGFGIAAPQILPFIQYIPHSPRAGGYSVGFAGSTSYGIPWNHIPEFIIAGFTG
                     FDFETYFGSNPLKLHSEYLGLPVVALAILGFGDQRRRLVWWVGGIGLLFLLIALGAST
                     PFYHLWWAVMPYVKKTRAPGMVFFIVAFCTAIFAAFGVSRIERGEAAAARHTRIWLIA
                     AGVIALFGLIGVWGQLAQSIAPPERLAKAIARGPFIRVSAVLGALALGAIGLLVWGRL
                     KGRVPVLLFALGLPLVVGADLWRNGKLFWQYAPAPDKGLYRTDPLVARLQAEPKPFRV
                     LDLGPFVQGIGVYPQNVLMAFDVAQVVGYQGSELRYYDELLGGRPGAAQPQYLLTSTR
                     LWDLLAVRYVVVPDTLQIPGYHKVLGPIANAAGNRGYLYEADTTPAYVRVVPAAVKLS
                     ADSLVPPTLADPRLPGYDRVVLIPTSAPINPAPLRAIPPASASHGKVTNWGPGRMSIT
                     LDPPPRDSSYV"
     gene            complement(2155..>2487)
                     /locus_tag="VEM33_07810"
     CDS             complement(2155..>2487)
                     /locus_tag="VEM33_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51771.1"
                     /translation="VFALLLSLQWLAYGAAFRLLAIGLLGSQGGAGQASWGFYITAFT
                     ESYLAGVIAVFAPAGLLVREAALISVLTPVLGGPDAVILAVAARIWHTALELVSGALV
                     LAIPVPRP"
BASE COUNT          504 a          788 c          835 g          360 t
ORIGIN      
        1 acgtagctgg agtcgcgcgg tggcgggtcc agggtgatcg acatccgccc cggtccccag
       61 ttggtcacct tgccgtggga cgccgaggca ggcggtatcg cgcggagcgg cgccgggttg
      121 attggggcgc tcgtcggaat cagtaccacc cgatcgtatc cgggtagtcg cggatcggcg
      181 agcgtcggcg gcacgagcga atccgcagag agcttcaccg ccgccggcac gactcgcacg
      241 taggctggcg ttgtgtcggc ctcgtacaag tagccgcggt tgccggccgc gttcgcgatc
      301 gggccgagca ccttgtggta tccgggaatc tgtaaggtat ccgggaccac cacgtaccga
      361 actgcgagca agtcccacag tcgcgtggac gtgagcagat attgcggctg ggctgcgccc
      421 ggccggcccc ccagcagctc atcgtaatac cgcagctcgc tgccttgata acccacgacc
      481 tgggcgacgt caaatgccat caggacgttc tgcgggtaca cgccgatgcc ttgcacaaag
      541 ggcccgaggt cgagcacgcg gaacggcttc ggttccgctt gcaggcgcgc aacgagcgga
      601 tcggtgcggt acaggccttt gtccggggcc ggcgcgtact gccagaagag cttgccattg
      661 cgccagagat ccgctccgac gaccagcggc aggcccagcg caaacaggag caccggtacg
      721 cggcccttga gccgtcccca cacgagtagg ccgatcgctc ccagtgcgag cgcgcccagg
      781 accgccgaga ctctgatgaa cggtccgcgc gcgatggctt tggcgagtcg ctcaggtggc
      841 gcgatactct gcgcgagctg accccacacg ccgatgaggc cgaagagcgc gatcaccccg
      901 gctgcgatca gccagatgcg ggtgtgtcgc gccgcggccg cttctccccg ttcgatgcgc
      961 gagacgccga acgcggcaaa aatcgccgta cagaaggcca cgatgaagaa gaccatgccc
     1021 ggcgctcgtg tcttcttgac gtagggcatc accgcccacc acagatggta gaacggcgtg
     1081 gacgcaccca gcgcgatgag gagaaacagc aagccgatgc cgccgaccca ccagacgagc
     1141 cggcggcgct gatcgccgaa gcccagaatg gcgagggcca cgaccgggag tcccaggtac
     1201 tcggagtgca gcttgagcgg attcgatccg aagtacgtct cgaagtcaaa accggtaaag
     1261 cccgcgatga tgaactcagg gatgtggttc cacggaatcc cgtaggatgt ggatcccgca
     1321 aatccgacgg agtagccccc tgccctgggc gaatggggga tgtattgaat gaagggcagg
     1381 atctgcggcg ccgcgatgcc gaagcccacg accactgcga caaacacgat cccgagtctg
     1441 atcaggcgac tgcgcaatgg gtcgggactc ggctctccga acgtcaagta cagcgcgaac
     1501 agcgccgcgg cgatcaagag gtaataggtc gtctgtacgt gcgggctcag caaacagaga
     1561 gcgacggcga cggcgagcaa cggatagccc caggcgcgtt tgtcccgcag tgccgccagc
     1621 agcgcgagga acgccagcgg tagcatcgtc gagacaaaga gcttgccgtc gtgtcccggg
     1681 tggacgtacg agatcaggat gccggacagc tggtacgcga ctcctcccgt caccgcgccg
     1741 atccacgacg cgcccaaccg ccgcagaaac aggtacgtca gaaaacctgc gaggatgtag
     1801 tgcaggacga acgcgatgtt catcgcctgg tacggcggca tcacctcgcg caagaacgag
     1861 gtgggataca gtacgtcgcc gtgcgtgacg acgtcgatgt gcggcagtcc acccatgatc
     1921 atcggattcc agagcgggaa gtgcccggtc cggtggtact cgttcgcggc ccattccttg
     1981 accgcgtagc cgctcgagta ttgatcgccg tccggcgacg cgagccattt ccccgagagc
     2041 atcggcaacg cgagaatggc gatgaagagc gataccacac ccaacgccgc gagatgcggg
     2101 cgtcgaggtg cgtaaggacc gattgctggc gtgggcgatg ctgtcatggc agggttatgg
     2161 cctgggcaca gggatggcga ggaccagcgc gcccgacacc agctcgagtg ccgtgtgcca
     2221 gatccgcgcc gcgacggcga gaatgacggc atcggggccg ccgagcaccg gcgtcaggac
     2281 gctgatgagt gcggcttcgc gcaccagcaa cccggccggg gcgaagacgg cgatcactcc
     2341 agcgagatag ctttccgtga aagcggtgat gtagaacccc cacgaggcct gtccggcgcc
     2401 tccctgactg cccagcagcc ctatcgccag aagccgaaac gcggcgccat aggccagcca
     2461 ttggagcgag agcagcaggg caaaaac
//
LOCUS       DASPZD010000403         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1328401, whole genome shotgun sequence.
ACCESSION   DASPZD010000403 DASPZD010000000
VERSION     DASPZD010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328401"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..2516
                     /locus_tag="VEM33_07815"
     CDS             150..2516
                     /locus_tag="VEM33_07815"
                     /inference="COORDINATES: protein motif:HMM:NF006101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HYS51772.1"
                     /translation="MKISIIGGGPGGLYFALLAKKRWPRWEIAVHERNRADDTFGFGV
                     VFSDATLGTFHDYDPPSYESIRRSFAYWDDVEIVYKGRALRCAGNGFCGCSRVTLLKL
                     LQARCREVGVDLRFQTEASVEDFPDSDLIVAADGINSRTREAYSEHFKPSVDLRPNKF
                     VWLGSTRPLDAFKYFFKETEHGILLAHCYQYEKDASTWIIETNADTWERSGFDRMDEA
                     QMLRALELLFADELGGHRLIANRSQWRNFPNIRNSTWVMDKVVLVGDAKATAHFSIGS
                     GTKLAMEDAIALFDSLRTEGGVTGALTLYDSQRREDVEKTQHAADVSLAWFENMRRYW
                     TMDPPQFAFGVISRSKQITYENVRLRDPEFLEGVTRWFADTVLPPGFEVHLRNPPPPM
                     FTPFALRGMRLENRVVVSPMDQYMAVDGIPNDWHLVHLGSRAVGGAGLVFVEMTCTSP
                     EARISPGCTGLWNEVQRDAWKRIVDFCHANSGAKLCMQIGHSGRKGSTQLGWERTDYP
                     LESGNWELVAPSPLPYHSESQTPREMTRADMDRIRDDFVRSARYADAAGFDMLELHMA
                     HGYLLSSLISPLTNVRKDEYGGSIRNRLRYPMEVFRAVRAVWAQEKPMSIRISATDWA
                     PGGLSEDDLLELARAFKEAGADLIDVSTGQVVPHQKPVYGRMYQTPFADKIRNEIGLA
                     TMAVGNITTADQVNTIVVSGRADLVALARPHLADPYFTLHAAAWYQHPGQFWPKPYLP
                     GRDQAYRLAVRDREEWTAGRQATKPPSHEVKEEQYQQQSGGGPGKKAA"
BASE COUNT          487 a          899 c          874 g          448 t
ORIGIN      
        1 gcgatcggtc cggcgtaccg cgagctgatg ggaaagcact acccggcgat ggcggtcgtc
       61 caggtgagcg gcctcgtcga ggccctcgcg cgcctggaga tcgaggcgac ggcggtgatt
      121 ccggactcat tccaggctga ggccaaggcg tgaagatcag cattatcggc ggcgggccgg
      181 gcgggctgta cttcgcgctg ctcgcgaaga agcgatggcc gcggtgggag atcgcggtcc
      241 acgagcgcaa ccgcgccgac gataccttcg gcttcggcgt ggtgttctcc gacgcgacgc
      301 tcgggacgtt ccacgactac gatccgcctt cgtacgaatc gatccgccgc agcttcgcct
      361 actgggacga cgtcgagatc gtctacaagg gccgcgccct gcgctgcgcg ggaaacggct
      421 tctgcggctg ctcgcgcgtc acgcttctga agctcctgca ggcgcgttgc cgcgaggtgg
      481 gcgtcgattt gcgcttccag acggaggcga gtgtcgaaga cttcccggac agcgacctca
      541 tcgtcgccgc ggacggcatc aattcgcgca cgcgcgaggc ttacagcgag cacttcaagc
      601 cctcggtcga cctgcggccg aacaagttcg tgtggctggg ctcgaccagg ccgctcgatg
      661 ctttcaagta tttcttcaag gagaccgagc acggcattct gcttgcgcac tgctatcagt
      721 acgaaaagga cgcgagcacc tggatcatcg agaccaacgc agacacctgg gagcgctcgg
      781 gcttcgacag gatggatgaa gcgcagatgt tgcgcgcgct ggagctcctc ttcgccgacg
      841 agctcggcgg ccacaggctc atcgccaacc gttcccagtg gcgcaatttt cccaacatcc
      901 gcaattccac ctgggtcatg gacaaggtgg tgctggtggg cgacgccaag gccaccgcgc
      961 acttctccat cggctcgggc accaagctcg cgatggagga cgcgatcgcg ctgttcgact
     1021 cgctgcggac cgagggcggc gtcacgggcg cgctgacgct ctacgattcg cagcgacgcg
     1081 aggacgtcga gaaaacgcag cacgcggcgg acgtgtcgct cgcgtggttc gagaacatgc
     1141 gccgctactg gaccatggat ccgccccagt tcgcgttcgg cgtcatttcg cgttccaagc
     1201 agatcaccta cgagaacgtg cgactgcgcg atccggagtt tctcgaaggc gtcacgcgct
     1261 ggttcgcgga cacggtcctg ccgcccgggt tcgaggtcca tttgcgcaac ccgccgccgc
     1321 cgatgttcac acccttcgcc ctgcgcggca tgcgcctcga gaaccgcgtc gtcgtctcgc
     1381 ccatggacca gtacatggcg gtggacggga taccgaacga ctggcacttg gtgcatctcg
     1441 gcagtcgcgc agtcggcggc gcggggctcg tgttcgtcga gatgacctgc acttcgcccg
     1501 aggcgcgcat ttcgccggga tgcacgggcc tgtggaacga agtgcaacgc gacgcctgga
     1561 agcgcatcgt cgatttctgc catgcgaact ccggcgcaaa gctgtgcatg cagattggcc
     1621 actccggacg caaaggctcg acgcagctcg gctgggagcg cacggactac ccgctggaat
     1681 ccggcaactg ggaactggtc gcgccttcgc ctcttcccta ccattcggaa tctcaaaccc
     1741 cgcgcgagat gacccgcgcc gacatggacc ggatccggga cgatttcgtg cgctccgccc
     1801 ggtacgccga cgcggccggc ttcgacatgc tcgagctgca tatggcgcac ggttacctgc
     1861 tctcgtcctt aatctccccg ctcacgaacg tgcgcaagga cgaatacgga gggtcgatcc
     1921 ggaaccggct gcgctatccg atggaagtgt tccgtgcggt gcgcgcggtc tgggcgcagg
     1981 agaaacccat gtcgatccgc atttccgcca ccgactgggc gccggggggc ttgtcggaag
     2041 acgacctgct cgagctcgcg cgcgcgttca aggaagccgg cgccgatctg atcgacgtct
     2101 ccaccggcca ggtcgtaccg caccagaagc cggtatacgg gcgcatgtac cagacgccgt
     2161 tcgccgacaa gatccgcaac gaaatcgggc tcgccacgat ggcggtcggc aacatcacca
     2221 ccgccgacca ggtgaacacc atcgtcgttt cagggcgcgc cgacctggtc gcgctcgcgc
     2281 gcccgcacct cgccgatccc tacttcacgc tgcacgccgc cgcgtggtac cagcacccgg
     2341 ggcagttctg gcccaagcct tatctgccgg gacgcgacca ggcttaccgg ctcgcggtcc
     2401 gcgaccggga ggaatggact gcgggacgcc aggcgacgaa accgcccagc cacgaagtga
     2461 aggaagaaca ataccagcaa caaagcggcg gcggacccgg caagaaggcc gcttgaggtc
     2521 ttgccgggtg cagtgccgct cggttgactg ctaatcgccc tgcggaacca gcgcgaaggc
     2581 gcgcagcgcg gagcccgtgc cgccgagaat cttcaaaggc ggaaccacga ccacgaattc
     2641 atacgccttc gcctgcgcca actcctcgag gttcaggttc tcgataatgt agatcccgga
     2701 gtcggcga
//
LOCUS       DASPZD010000404         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_312766, whole genome shotgun sequence.
ACCESSION   DASPZD010000404 DASPZD010000000
VERSION     DASPZD010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312766"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VEM33_07820"
     CDS             complement(<1..238)
                     /locus_tag="VEM33_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYS51773.1"
                     /translation="MQSLTLTRPDDAHLHLRDGAALASVLPHTARAFARAVVMPNLRP
                     PITDTALAAAYRGRVLKALPKGSGFEPLMTLYLTD"
     gene            complement(243..1076)
                     /gene="rsmI"
                     /locus_tag="VEM33_07825"
     CDS             complement(243..1076)
                     /gene="rsmI"
                     /locus_tag="VEM33_07825"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYS51774.1"
                     /translation="MATPIGNLEDISPRALHSLAHADAIAAEDTRVTGRLLEHHRLQG
                     KLIAVHGHNEQRAADSIVEQLARGRIVALVTDAGTPAISDPGATVVKKARAAGFRVIP
                     IPGPNAAVTALSAAGIADGAFLFAGFLPAKPGLRRKALDRLGALPYTLVFYEAPHRVV
                     ECVEDMSAVLGPERVLVIARELTKLFEQIHRCVLGEAAAWLREDTDRQRGEFVLIAEG
                     AAARSESAKLDWERVLTTLLAELPLAHAVKLACKLTGAKKNAVYARALQLARAANARR
                     S"
     gene            1236..2180
                     /locus_tag="VEM33_07830"
     CDS             1236..2180
                     /locus_tag="VEM33_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HYS51775.1"
                     /translation="MLPLNAPEFSRAAEAVRLGCQAALVFAENRQSLQVARTDAQPES
                     ILAEYQAAVQRGAAVIVGPLTRSGVTALAQAGRVSVVTLALNVADGDAPLPPRLYTFG
                     LSAETEARAVARTAFARGLREGAVVQASNLLAKRLSRAFADEWFSLGGRISDVREFGA
                     RADLVDLRRGLADSQAQFIFLAAEADQARTVRPYLNNQLQVFATSQVNDGKNDPLVNA
                     DLNGIRFVDMPWLVQPDHAAVMVYPRLEGLSTELQRFYALGIDSCRIAAELIGGQQTI
                     NLDGVTGRISYRGGNTLQREPIQAVFRDGMGVSLENAR"
     gene            2188..2526
                     /locus_tag="VEM33_07835"
     CDS             2188..2526
                     /locus_tag="VEM33_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYS51776.1"
                     /translation="MKAGDRAEQIAAGYLQRKGLALIETHYRCRWGEIDLILRDLDTV
                     VFTEVRLRRSKGFGGAAYSVDARKQARIIATARHYLAGKKEAACRFDVVLLDRLEPPR
                     VEWIRDAFSA"
     gene            2620..>3170
                     /locus_tag="VEM33_07840"
     CDS             2620..>3170
                     /locus_tag="VEM33_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HYS51777.1"
                     /translation="MKAVTARPAEDSVDLLARIRRQFDDSAQLKLKAHDVLAEPIVRA
                     TEIMVKCLLVNGKILACGNGGSAADAQHFSAELLNRFERERPGLAAIALTTDTSTLTA
                     IANDYEYEQVFSKQLSALGQAGDVLLGISTSGNSKNVARAVSAAHEREMRVVALTGRG
                     GGEIGKLLASEDVHICVPHSQTAR"
BASE COUNT          535 a         1057 c         1046 g          532 t
ORIGIN      
        1 cgtcggtcaa atagagggtc atgaggggct cgaaaccgga gcccttcgga agcgccttca
       61 gcacgcggcc gcggtaggcg gcggcgagcg cggtgtccgt aatcgggggc cggagattcg
      121 gcatcacgac ggcgcgcgca aatgcgcgcg ccgtgtgggg gagcacgctc gcgagcgcgg
      181 cgccgtcgcg aaggtgcaag tgagcgtcgt cgggccgggt gagagtcagg gattgcacgg
      241 aatcaggatc gccgggcgtt cgcagcgcga gccagttgca aggcgcgcgc gtacacggcg
      301 ttcttcttcg ctcctgtgag cttgcacgcg agcttcaccg catgcgcaag cggcagctcc
      361 gcgaggagcg tggtgagcac gcgttcccaa tcgagcttcg cgctttcgct tcgggccgcg
      421 gcgccctccg cgatcaggac gaactcgccg cgctggcggt cggtgtcctc gcgcagccag
      481 gccgccgctt cgcccaagac gcagcgatga atctgctcga agagcttcgt cagctctcgc
      541 gcgatcacga ggacgcgttc gggacccagc accgccgaca tgtcctcgac gcattcgacg
      601 acgcggtgag gcgcctcgta gaacacgagc gtgtacggca aggcgccaag acgatcaagc
      661 gccttgcgcc tcaggcccgg ttttgcagga agaaaacctg cgaatagaaa cgcgccgtcc
      721 gcgatgcccg cggccgagag cgcggtgacg gcggcgttcg gccccggaat cgggatcacc
      781 ctgaatccgg cggcgcgcgc ttttttgacg accgtcgcgc ccggatcgga tatcgccggc
      841 gtgcccgcgt ccgtgaccag cgcaacgatc ctgcctcggg cgagctgctc gactatggaa
      901 tcggcggcac gctgttcgtt atggccgtgc accgctatca gctttccctg caggcgatgg
      961 tgctcgagca ggcgccccgt cacgcgcgtg tcctccgctg cgatggcgtc ggcatgagcg
     1021 agcgaatgaa gggcgcgcgg gctgatatcc tcgaggtttc caatgggcgt ggccaccaca
     1081 tataattgac ctgccccact cgggctccca ctatgcttcc ccgtcctcgc gcccaagtct
     1141 gcttcgctct gttggcgttg ttgagtccgg cattgtgcgc ggccgtcgac gatccggcaa
     1201 gcagaccccc ggacgctcgg cctttcatag gcctgttgct tcccctcaat gcgccggagt
     1261 tctcacgagc ggccgaggcg gtgcgcctgg gctgccaggc cgcgctcgtc ttcgcggaga
     1321 accgccagtc cctgcaggtg gcgcgcaccg atgcccaacc cgagagcatc ctcgccgaat
     1381 accaggccgc ggtgcagcga ggcgccgccg tgatagtcgg cccgctgacg cgctccgggg
     1441 tcacggcgct cgctcaagcg ggccgcgtca gcgtggtcac gctcgccctg aacgtcgccg
     1501 acggcgacgc gccgctccca ccgcgactct acactttcgg actttccgcc gagaccgaag
     1561 cacgcgcggt cgcgcgcacg gctttcgcgc gcgggctgcg cgagggcgca gtcgtgcagg
     1621 cgtcgaattt gctcgccaag cgcctgagcc gggcgttcgc ggacgaatgg ttctcgctcg
     1681 gcggaaggat cagcgacgtg cgggaattcg gtgcgcgagc ggacttggtc gacttgcgtc
     1741 ggggtctggc ggactcgcag gcacaattca tttttctcgc cgcggaagcc gaccaggcac
     1801 gcaccgtgcg accctatctg aacaaccagc tccaggtttt tgcgacctcc caggtcaacg
     1861 acggcaagaa cgatccgctc gtcaacgcgg atctgaacgg gattcgcttc gtcgacatgc
     1921 cttggctcgt gcaaccggac cacgctgcgg tcatggtcta tccgcgcctg gagggccttt
     1981 ccaccgagct tcaacgtttt tacgcgctcg gcatcgactc gtgccgcatc gcggcggagc
     2041 tgatcggggg gcaacaaacc atcaacctgg acggcgtgac tggcaggatc tcctaccgcg
     2101 ggggcaatac gcttcagcgc gaacccatcc aggcggtgtt ccgcgacggc atgggtgtgt
     2161 cgcttgagaa cgcgagatga aggcgagatg aaggccggcg atcgtgccga gcagatcgcg
     2221 gcgggctatt tgcagcgcaa gggcctggcc ttgatcgaaa cccattaccg ctgccgctgg
     2281 ggcgagatcg atctcatcct gcgggatctg gataccgtcg ttttcaccga agtcaggctg
     2341 cggcgctcca agggctttgg gggcgccgct tacagcgtcg atgcgcgcaa acaggcgcgg
     2401 atcatcgcca cggcgcgcca ctatcttgcg ggcaagaagg aagcagcttg ccggttcgac
     2461 gtcgtgctcc tcgatcgcct cgaaccgccg cgggtcgaat ggatacgcga cgcattctcc
     2521 gcctgagccc ctgcctggct acgcttcacg cacgcccgcg gtgctgcccc agccggcccg
     2581 gcgcgtccgc gaggaaggtt acggtagaat aagggtcgta tgaaagccgt caccgcccgc
     2641 ccggccgagg acagcgtgga ccttctggcg cgcatccgcc gccagttcga cgacagcgct
     2701 caactgaaac tgaaagcaca cgacgtgctt gccgaaccga tcgtccgcgc cacggaaatc
     2761 atggtgaaat gtctgctcgt caacggcaag atcctcgcgt gcggtaacgg cggctcagcc
     2821 gccgacgccc agcatttttc cgctgagctg ctcaaccgct tcgagcgcga gcgtcccggg
     2881 ctcgccgcga ttgcgctgac gaccgacacc tccaccttga ccgcgatcgc aaacgactac
     2941 gaatacgagc aggtattctc caagcagctg agcgcgttgg gtcaggcggg tgacgtgctc
     3001 ctcggcattt ccacgagcgg caattcaaaa aacgttgctc gcgcggtctc ggcggcgcac
     3061 gaacgcgaga tgcgcgtggt cgccctgacc ggccgcggcg gaggcgagat cggaaagctg
     3121 ctcgcgtcgg aagacgtgca tatctgcgta ccccactcgc aaaccgcccg
//
LOCUS       DASPZD010000405         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_763721, whole genome shotgun sequence.
ACCESSION   DASPZD010000405 DASPZD010000000
VERSION     DASPZD010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763721"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VEM33_07845"
     CDS             <1..706
                     /locus_tag="VEM33_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYS51778.1"
                     /translation="LVVFFHDQPLTPAQFMAFARRMGKPIEYPLVKGIAGFPEVIEVK
                     KLEHERHNFGGLWHSDTAYLENPPMGSMLLAREVPPYGGDTEFANQYGAYETLSEGMR
                     KLLDGLVAINASTKADATRTREDRMKEQDHETRQFLAEHPVVRTHPETGRRALYVNVG
                     HTVRFKDMTEEESAPLLEFLYRHQVRPELTCRFRWRVGSLAFWDNRCTQHNPLNDYHG
                     HRRVMHRITLAGDKPC"
     gene            complement(811..1563)
                     /locus_tag="VEM33_07850"
     CDS             complement(811..1563)
                     /locus_tag="VEM33_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HYS51779.1"
                     /translation="MIKTEIRERIATIELARLDKKNALTAAMYAAMTDTLATAESDSG
                     VRAIVIHGTRDCFTAGNDLKDFLEGPAGASQALRFVSALSKVCKPVVAAVGGPAVGIG
                     TTLLLHCDLVYAAPGARFQLPFVPLGLVPEAASSLLLPRVAGYQRAAAWLLLGQVFTA
                     SEALAAGIVTEIVPEETLIERARAAAAALAALPPESVRLTKQLMKQPVAADVAKQMAE
                     EARLFAERLQSAEAKEAMAAFIEKRKPDFSRF"
     gene            complement(1560..1928)
                     /locus_tag="VEM33_07855"
     CDS             complement(1560..1928)
                     /locus_tag="VEM33_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51780.1"
                     /translation="MKVFVDDRRLARDIVRHEVKTAHQMGWSTIENGELLALAAKEFD
                     VFVTVDRNLSFQQHLPAFEIAVIVLRAVSNRLADLQPLIPELLVSIPKAKRGAVTYVG
                     DGLQRGSTLNSQATQPSLPR"
     gene            complement(1925..2137)
                     /locus_tag="VEM33_07860"
     CDS             complement(1925..2137)
                     /locus_tag="VEM33_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYS51781.1"
                     /translation="MGKPVISCDPDVMGGTPVFSGTRVPVQTLLDYLESGESIDDFLA
                     GFPSVNREQVIDFLEQAKDRLVDTAS"
BASE COUNT          419 a          717 c          708 g          379 t
ORIGIN      
        1 cttggtggtg ttctttcacg accagccgct cacccccgcc cagttcatgg cctttgcacg
       61 gcgcatgggc aagccgatcg aatacccgct cgtcaagggc atagccggat tcccggaggt
      121 catcgaagtg aaaaagctcg agcacgagcg gcataacttc ggcggcttgt ggcattccga
      181 caccgcctac ctcgagaatc cgccgatggg ctcgatgctg ctcgcgcgcg aggtcccgcc
      241 ttacggcggg gacaccgagt tcgccaatca gtacggggcc tatgagactc tgtccgaggg
      301 catgcgcaag ctgctcgacg gcctggtggc catcaacgct tcaaccaaag ccgatgccac
      361 gcgtacgcgt gaggaccgca tgaaggaaca ggaccacgag accaggcagt tcctcgccga
      421 gcaccctgtc gtccgtaccc atccggaaac cgggcgcagg gcgctctacg tgaacgtcgg
      481 ccacaccgtg cgcttcaagg acatgaccga ggaagaaagc gcgccgctgc tggagttcct
      541 gtaccggcac caggtgcgac ccgagctcac ctgccggttc cgctggcgtg tcggctcgct
      601 cgcgttctgg gacaaccgct gcacgcagca caaccccttg aacgactatc acgggcatcg
      661 gagggtgatg catcgcatca ccctcgcggg cgacaagccg tgctgatcca gcgcgaaatc
      721 atcaaatgtg gacctcaagc cttgcgggat tcaagccgcc gctcgatcaa gaggacggcg
      781 agaattccgc gtgacacgac cgcgtcctcg ctagaaccgc gagaagtcgg gtttgcgctt
      841 ttcgatgaac gcggccatcg cttccttcgc ttccgccgac tgcagccgtt cggcgaacag
      901 gcgtgcttcc tcggccatct gctttgcgac gtccgccgcg accggctgct tcatcaattg
      961 cttggtcaag cgcacggact cgggcggcag cgcagcgagc gcggctgccg cggcgcgcgc
     1021 gcgctcgatc agggtttcct ccggcacgat ctcggtgacg atgccggcgg ccagggcttc
     1081 gctcgcggtg aacacctgcc cgaggagcag ccacgcggcg gcgcgctggt aacccgcgac
     1141 cctcggcagc agcaggctcg aagccgcctc gggcaccagg ccgagcggaa cgaaaggcag
     1201 ctgaaagcgg gcgcccggcg ccgcgtacac cagatcacag tgcagaagca gagtggtgcc
     1261 gattcccacc gccggccctc cgaccgcggc gaccacgggc ttgcagactt tcgacagcgc
     1321 cgagacaaag cgcagcgcct gcgaagcgcc agccggacct tcgagaaagt ccttgaggtc
     1381 gttgccggcg gtgaagcagt cacgggtacc gtggatcacg atcgcgcgga cccctgaatc
     1441 ggactccgcg gtggcgagcg tatcggtcat cgcggcatac atcgcggccg tgagcgcatt
     1501 cttcttgtcg aggcgcgcca gctcgatcgt cgcgatgcgc tcccggattt cggtcttgat
     1561 catcggggaa gagagggttg tgtcgcctgc gaattcagcg tcgacccccg ttggagtccg
     1621 tctcctacgt aggtgacggc acctcgtttt gccttgggaa tcgacaccag caattcggga
     1681 atcagcggct gaagatcggc gaggcgatta gaaactgcgc gcaacacgat gaccgcgatc
     1741 tcgaaggcag ggaggtgctg ctggaaggag aggtttcggt cgacggtgac gaacacgtcg
     1801 aactcttttg cggccagcgc aagcagttcg ccgttctcga tggtcgacca tcccatctgg
     1861 tgcgccgtct tgacttcgtg gcggacgatg tcgcgcgcga gccgccgatc gtcgacaaat
     1921 accttcacga ggcggtgtcg accaggcggt cctttgcttg ctcgaggaaa tcgatgacct
     1981 gctctcgatt gacggaggga aatcccgcca gaaaatcgtc gatggactct ccggactcca
     2041 ggtagtcgag gagcgtctgg accggcacgc gggtgccgga aaagaccggg gtgcccccca
     2101 ttacgtcggg gtcgcaggaa atgactggct tgcccatggc gtgagactac tcccgactcc
     2161 gagacccgcg caacattagg aaactcaagc cttgcgggat tccagccgcc gctcgcggtt
     2221 gcg
//
LOCUS       DASPZD010000406         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1607084, whole genome shotgun sequence.
ACCESSION   DASPZD010000406 DASPZD010000000
VERSION     DASPZD010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607084"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VEM33_07865"
     CDS             <1..389
                     /locus_tag="VEM33_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYS51782.1"
                     /translation="ERTDLYSLGVILYEMLTGKVPWSGDSAQQVMHAKLEEDPTPPRA
                     LRPEIPPALEEVVLHALERRPERRPESALELREALAHLDSVVITNRAARLRRARRLPR
                     WARVLLRVGGAAAAYGLLLWALSHVG"
     gene            430..558
                     /locus_tag="VEM33_07870"
     CDS             430..558
                     /locus_tag="VEM33_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51783.1"
                     /translation="MPALQARPARLDAAARLGGGIVLAPLAGVALGWPGKTTSPPW"
     gene            complement(658..1137)
                     /locus_tag="VEM33_07875"
     CDS             complement(658..1137)
                     /locus_tag="VEM33_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51784.1"
                     /translation="MSHSSGRFNWRMPGASVTNVPLTAAAGRVHTSPKMAWALQNSPS
                     MITDTRCWRRLTPETITELPETAAVFEVANLVRTVQFIGRASGNLRARMATYMSEDLK
                     LRPCTGGYFFRYEPTASEEEALDRRLASYLDSHGGLLPAGNAGPQPGLRLAARRVAA"
     gene            1149..2804
                     /gene="scpA"
                     /locus_tag="VEM33_07880"
                     /pseudo
     CDS             1149..2804
                     /gene="scpA"
                     /locus_tag="VEM33_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            2808..3209
                     /locus_tag="VEM33_07885"
     CDS             2808..3209
                     /locus_tag="VEM33_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HYS51785.1"
                     /translation="MPEKKIRVLIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEMIASAAVQEDVDVIGLSILSGAHNTICPQLMNLLREKGMTDVIVLVGGIIPEADIS
                     VLKKSGIAEIFLPGTSTQDIVDFIHKRVSPA"
     gene            complement(3206..4279)
                     /gene="rsgA"
                     /locus_tag="VEM33_07890"
     CDS             complement(3206..4279)
                     /gene="rsgA"
                     /locus_tag="VEM33_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HYS51786.1"
                     /translation="MSILRWGWNGYFEAAWKNAERENAVPARVIAQGRGIWRVACDFG
                     ECPADASGKLRLAAEEGADWPAVGDWVAAELRDQGRAALIHGVLPRRSQFVRKMAGKK
                     IAEQVIAANVDTSLLVSALDGDFNPRRVERYLAQCWESGAKPVIVLNKADACGKPQEK
                     AAEVEKVAMGVPVCLVSARTGQGFDDLQKVFAHGQTLALLGSSGVGKSTIVNRLMGRA
                     IQEVHEVRESDSRGRHTTTSRQIFVLPGGALLVDTPGLRELQLWEVDEGISQAFADID
                     ALAAQCRFTDCGHATEPGCAVQADIQAGTLDLERLENWRKLQREQEFLRRKIDPEARH
                     NEKQRIKLLMRGVRQMYQDRKKS"
BASE COUNT          803 a         1557 c         1353 g          767 t
ORIGIN      
        1 acgagcgcac cgacctctac agcctggggg tcatcctcta cgagatgctg accgggaagg
       61 tcccctggtc gggcgacagt gcgcagcagg tcatgcacgc caagctggag gaggacccca
      121 cgccgccgcg cgcgctccgg cccgagatcc cgccggccct ggaggaggtc gtgctgcacg
      181 cgctcgagcg gcgccccgag cggcgccccg agagcgccct cgagctgcgc gaggcgctcg
      241 cgcacctgga cagcgtggtg atcacgaacc gcgccgcgcg cctgcgccgc gcgcgtcggc
      301 tcccgcgctg ggcgcgcgtg ctcctccgcg tcgggggcgc cgcggcggcg tacggactcc
      361 tcctctgggc cctgtcccac gtcggttaga gctcgcggag tcggagctgc ccgccgcgca
      421 ggcagacgcg tgcccgccct gcaggcgcga cccgcacggc tcgacgcggc agcgcgcctc
      481 ggcggcggca tcgtccttgc tcctctcgcc ggcgtggctc tcggctggcc cggcaagacg
      541 accagtcccc cctggtagcc ggcgtgcggc ggactcggcg gatgcgctcg ggttcgccgc
      601 cgtcagccgg ggctcacgcg tacagatctg ggcgcgaagc tccggaaaac tgacggctca
      661 ggcggcgacg cggcgcgcgg cgaggcggag gcccggctgg ggcccggcgt tgcccgccgg
      721 cagcaagccg ccgtggctgt ccaggtacga ggcgaggcgc cggtcgagcg cctcctcctc
      781 ggacgcggtc ggctcgtagc ggaagaagta gccccccgtg cacggccgga gcttcagatc
      841 ctcgctcatg taggtggcca tgcgggcgcg caggttgccc gaggcgcggc cgatgaactg
      901 cacggtgcgc acgaggttcg ccacttcgaa caccgcggcc gtctcgggca gctcggtgat
      961 ggtttccggc gtcagacgcc gccagcaacg ggtgtcggtg atcatggagg gtgaattttg
     1021 caacgcccat gccatcttcg ggctcgtgtg gacgcggccg gcagccgcgg tgagcggcac
     1081 gttcgtgacg ctagcgccgg gcatgcgcca attgaaccgg ccgctggaat ggctcatgtt
     1141 acgaggccat ggcggaccgc accgaccctg caggcccggc gccctgggtc gagctggcgc
     1201 gcaaggagcg caggggcgcc gatcccgacg gcctcgtgtg gcatacgccc gagggcatcg
     1261 acgtgaagcc cctctggacg cgggccgacg tcgagcacct cgacttcatc gacacgctcc
     1321 ccggcgtctt cccattcgtc cgcggcccgc gcgccaccat gtacgccggg cagccgtgga
     1381 ccatccgcta gtacgccggc ttctccaccg ccgaggagtc caacgccttc taccgcgcca
     1441 acctggccgc cgggcagatg ggcctctcgg tcgccttcga cctcgccacc catcgcggct
     1501 acgacagcga ccatccccgg gtggtcggcg acgtcggcaa ggccggcgtg gcgatcgact
     1561 cggtcgagga catgaagatc ctgttcgacg gcgtgccgct cgaccgcatg tcggtgtcga
     1621 tgacgatgaa cggcgccgtg ctccccgtgc tggcgagctt catcgtggcc ggcgaggagc
     1681 agggcgtgcc gcgcgccaag ctcaccggga ccatccagaa cgacatcctg aaggagttca
     1741 tggtccgcaa cacctacatc tacccacccg cgccctcgat gcggatcgtg gccgacatca
     1801 tcgccttcac ggcgcgcgag atgccgaggt tcaactcgat ctcgatctcc ggctaccaca
     1861 tgcaggaggc gggggcgacc tgcgacctcg agctcgcctt caccatcgcc gacgggctcg
     1921 agtacgtgcg cgccgcgctc tcgcgcggcc tcgacatcga cgagttcgcc ggccggctgt
     1981 cgttcttctt cgcgatcggg atgaacttct tcatggaggt ggcgaagctg cgcgcggcgc
     2041 gcctcctgtg ggcgacgctc gtgcagaagc acttcgcccc gaagcagccg gcctcgctca
     2101 tgctgcgcac gcactgccag acctcgggcg cgagtctcac ccagcaggat ccgcacaaca
     2161 acatcgtccg caccacgatc gaggccatgg cggccgtgtt cggcggcacg cagagcctgc
     2221 acaccaactc cttcgacgag gccctcggcc tgccgaccga gcgggcggcc aagctggccc
     2281 tgcgcaccca gcaggtgatc ggctacgagt ccggcatcgt cgacacggtc gaccccctgg
     2341 ccggctcgta cttcgtggag acgctcacca acgaagtcgc gcgcggcgca tgggattaca
     2401 tcgaaagaat tgacgccatg ggcgggatgg ttgccgccat cgagcgcggc tatccgcaac
     2461 gcgaaattgc cgaagcttcg tatcgttacc agatggctgt ggacaagaaa gagaaaatca
     2521 ttgtcggtgt gaacggtttt gtgtctgaag aaaagccgct cgaaaccctg cagatcgacg
     2581 aaaccgtagc gcatcgccaa gccgcgcggc tcagcaagct gcgcgccgag cgttctcaag
     2641 ctgaagtgga ccgcaggctc acctctctcc gcaacgccgc caaaggaaaa gaaaatctca
     2701 tgccgttcat cttcgacgca gtgaagtcat acgccaccct tggcgaaatc tgcgacgccc
     2761 tgcgcgacgt cttcggcacc tatgaagaag tcgccatcac ctaactcatg cccgagaaga
     2821 aaatacgagt attgattgcc aaaccggggt tggatggcca cgaccgcggc gcgaaggtca
     2881 ttgcgcgcgc gctgcgcgac gccggcatgg aagtcatcta caccggcctg cgccagacgc
     2941 cggagatgat cgcctctgca gcggtccaag aagatgtcga cgtgatcggc ctctctatcc
     3001 tttctggagc gcataatacg atttgcccac agctcatgaa ccttcttcgc gagaaaggaa
     3061 tgaccgacgt cattgttctc gtcggcggaa tcatccccga agccgacatc tccgtcttaa
     3121 agaagtccgg cattgctgaa atttttcttc ccggcacttc cacgcaagat atcgtggact
     3181 tcatccacaa acgcgtttcg cccgcttaac tcttctttct gtcttgatac atttgccgta
     3241 cgccgcgcat aagtagtttg atccgctgct tttcattatg ccgggcttcg gggtcaattt
     3301 tccggcgcag gaattcctgc tcgcgctgga gtttgcgcca attctccagc cgctcaagat
     3361 ccagtgtgcc tgcttggatg tcagcttgca cggcgcagcc cggctccgtg gcatggccac
     3421 aatccgtgaa ccggcactgc gcagcaagcg cgtcgatgtc cgcgaaagcc tgcgagattc
     3481 cttcgtccac ctcccaaagc tgcagctccc gcagccccgg cgtgtccacg agcaacgccc
     3541 cgcccggcaa aacgaatatt tgccttgacg tggtcgtgtg tcttccgcgg ctgtccgact
     3601 cgcgcacctc atgcacttcc tgaatggcgc gccccatcag gcgattgacg atcgtggact
     3661 tccctactcc cgaggatccc agcagagcaa gcgtttgccc gtgcgcgaaa accttctgta
     3721 aatcatcgaa tccttgcccg gttctcgcac tcaccaaaca aacgggaacg cccatggcca
     3781 ccttttccac ctccgctgct ttttcttgcg gcttgccgca ggcatcggcc ttgttcaaga
     3841 caatcaccgg cttcgcgccg gattcccagc actgcgccaa atacctttcg acgcgccgcg
     3901 gattgaaatc accatcgaga gcggacacca acagcgatgt gtccacatta gcggcaatca
     3961 cttgttccgc aatcttcttt ccggccatct tgcgaacaaa ctggctgcgg cgcggaagga
     4021 ctccgtgaat cagcgctgcc cggccctgat cccgaagctc cgccgcgacc cagtcgccca
     4081 cagcaggcca atccgcgcct tcctctgccg cgaggcgcag cttgccggac gcatcggccg
     4141 gacactctcc gaaatcacac gcaacccgcc aaatcccgcg tccttgagcg atcactcgcg
     4201 ccgggactgc gttctctcgt tccgcatttt tccaggctgc ttcgaaatag ccattccatc
     4261 cccatcgtag aatcgacact gccgtatcct cccgcggcaa tgacacaaca cacggccgca
     4321 gctagcgccc aagcaaaaca actcaaaggg aatgaattgg aaacgcagga gggagtgatt
     4381 ccgtcagttc gaacgatccg tggactcagt tcacaaagtt agtccaaggg agccgcggag
     4441 acacacccac ccgcaggaac cttggtatgc attcgcacag
//
LOCUS       DASPZD010000407         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1004184, whole genome shotgun sequence.
ACCESSION   DASPZD010000407 DASPZD010000000
VERSION     DASPZD010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004184"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..>2196
                     /gene="bglX"
                     /locus_tag="VEM33_07895"
     CDS             129..>2196
                     /gene="bglX"
                     /locus_tag="VEM33_07895"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HYS51787.1"
                     /translation="MPRVSYILICTTLVAVPASAQNLDSLLARMTLEEKLGQLNLLSS
                     GGRASPEQMQLVRAGKLGGLFNVMGAENTTPVQRIAVTESRLKIPLLFGLDVIHGYRA
                     IFPIPLAEAGSFDPEAAEATARAAGQEAAAFGINWTYAPMVDIARDPRWGRIAEGSGE
                     DPHLGSLMAAARVRGFQQNIFATAKHFAGYGAAEAGREYNETEISERTMREVYLPPFK
                     AAVDAGVASLMSAFNDISGVPASGNAWLTDTVLRREWGYTGFVVSDWTSVAELINHSV
                     AGSQAEAGRRALTAGVDMDMQSSIYVDSLAALVRSNRIPMAVVDSAVLRILRAKTRLG
                     LFRDPYRARTAPLTSELRALARRVAGESIVLLKNERSLLPLPKTGTIAVIGPLADNKE
                     EPLGPWHTQGRAEDVVSVLQGIKTKVPTVLYAQGSGIDDTSKAGFAAAVAAARQAKVA
                     VLVLGERGDMSGEAASRASLGLPGVQQQLLEAVAATGTPVVLILMNGRPLILEWASDH
                     VPAIVETWFLGIEAGNATADVLFGDVNPSARLPVTFPRVLGQVPLYYNHRNTGRPPDV
                     NNKFSSKYIDVPVTPRYPFGFGLSYTTFAYSNLKLSAARARVSDTITATVTVTNSGSR
                     EGTEVVQLYVRDEVASVSRPVRELKAFRRVTLKPGESRSVDLRVALRDLWFIGLDMKP
                     VVEPGTF"
BASE COUNT          382 a          722 c          720 g          372 t
ORIGIN      
        1 catctcccgt gagatacgag tgagtcacac aaacttgcca gcacaatccc cgggggcgct
       61 agggcttcgc ttgtcccgat ttgcgccgta acttctcctg actttccctc gccccgagag
      121 cgattcccat gccgcgtgtc agctacatcc tgatttgcac gaccctggtc gccgtccccg
      181 cctccgccca gaatctcgat tcgctcctcg cccgcatgac gctcgaagaa aaactcggcc
      241 aattgaatct cctgtcgtcc ggcgggcgcg cctcgcccga gcaaatgcag ctggtgcgcg
      301 cgggtaagct cggcgggctg ttcaacgtca tgggcgccga gaacacgacg ccggtgcagc
      361 ggatcgcggt gaccgagtcg cggctcaaga tcccgctgct ctttggcctc gacgtgatcc
      421 acggctatcg cgcgatcttc ccgatcccac tcgcggaagc aggctcgttc gaccccgagg
      481 ccgccgaggc gacggcgcgc gcggcgggtc aggaagccgc cgcgttcgga atcaactgga
      541 cctacgcacc gatggtggac atcgcgcgcg acccccgctg gggtcgcatc gcagagggct
      601 cgggcgagga tccgcacctc gggtcgctca tggccgccgc ccgcgtgcgc ggcttccaac
      661 agaacatctt cgcgacggcc aagcacttcg ccggttacgg cgcagcggag gccggacgtg
      721 aatacaacga aacggaaatc tccgagcgca ccatgcgcga ggtctacctg ccgccgttca
      781 aggccgcggt cgacgcgggc gtcgcaagtc tgatgtcggc attcaacgac atctccggcg
      841 tcccggcctc gggcaacgcc tggctcaccg atacggtgct gcggcgcgaa tggggctaca
      901 ccggcttcgt ggtgagcgac tggacgtcgg tcgccgagct gatcaatcac agcgtcgcgg
      961 gctcgcaagc cgaagcaggg cggcgtgcgc tgacggccgg cgtggacatg gacatgcaga
     1021 gcagcatcta cgtcgattcg ctcgccgcgc tggtgcgcag caatcgcatt cccatggcag
     1081 tggtcgattc ggcggtgctg cgcattctgc gcgcaaagac tcgcctcggg ctcttccgcg
     1141 acccctatag ggctcgaact gcgccactca cctctgaact gcgggcattg gcgaggagag
     1201 tcgctggaga gtccatcgtt ctgctcaaga acgagcgatc gctgctgccg ctcccaaaaa
     1261 ccggcaccat cgccgtcatc ggaccgctcg ccgacaacaa ggaagagccg ctcggaccct
     1321 ggcacaccca gggccgcgcc gaggatgtcg tctcggtgtt gcaggggatc aagacaaagg
     1381 tgccgacagt cttgtacgcg cagggatcgg gaatcgacga cacgtcaaag gcgggattcg
     1441 cggctgcggt cgccgccgct cgtcaggcca aagtcgcggt cctcgtgctc ggcgagcggg
     1501 gcgacatgag tggcgaagcg gcgagccgcg cctcgctcgg cttgcccggc gtgcagcagc
     1561 agctgctcga ggcggtcgcg gcgacgggca cgccggtcgt gctcatcctg atgaacggtc
     1621 ggccgttgat cctcgagtgg gcatcggacc acgtgccggc gatcgtcgag acgtggttct
     1681 tgggcatcga ggcggggaac gcgacggcag atgtgctgtt cggcgacgtg aatccgagcg
     1741 cgcgcttgcc ggtcaccttc ccgcgagtgc tgggccaggt gccgctgtac tacaatcacc
     1801 gcaacaccgg ccgcccgccc gatgtcaaca ataaattctc gtcgaaatac atcgacgtgc
     1861 cggtgacgcc gcgctatccg ttcggattcg gattgtcgta cacgacgttc gcgtacagca
     1921 atctcaagct cagcgccgcg cgtgcgcggg tgagcgacac gatcaccgcg actgtgaccg
     1981 tcacgaacag cgggagccgg gaaggtacgg aggtcgtgca gctctacgtg cgggacgagg
     2041 tagcgagcgt gtcacggcca gtccgtgagc tgaaagcctt ccggcgcgtg acgctaaaac
     2101 ctggcgagtc gcggtctgtc gatttgcggg tggcactgcg cgatctctgg ttcattgggc
     2161 tcgacatgaa gcctgtcgtc gagccgggaa cgttcc
//
LOCUS       DASPZD010000408         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_407901, whole genome shotgun sequence.
ACCESSION   DASPZD010000408 DASPZD010000000
VERSION     DASPZD010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407901"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..286)
                     /gene="grxC"
                     /locus_tag="VEM33_07900"
     CDS             complement(44..286)
                     /gene="grxC"
                     /locus_tag="VEM33_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HYS51788.1"
                     /translation="MYSTAVCPFCLRAEMLLGSKGVTEIEKIRVDLDPRQREEMMNRT
                     GRRTVPQIYIGDTHVGGFEELAALEHAGKLDSLLAD"
     gene            complement(302..709)
                     /locus_tag="VEM33_07905"
     CDS             complement(302..709)
                     /locus_tag="VEM33_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYS51789.1"
                     /translation="MKFVTDNIFLVGVAFVSGAMLVWPAVRRGASGASVSTLEATLLI
                     NQQNALVLDVRDAAEYEKGHLLNARNIPLGELEPRAGELEKHKAKPVIVVCDDGNRSG
                     KAATALRKQGFEQVFTLSGGIRAWRQAGLPLEK"
     gene            834..1583
                     /gene="gpmA"
                     /locus_tag="VEM33_07910"
     CDS             834..1583
                     /gene="gpmA"
                     /locus_tag="VEM33_07910"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HYS51790.1"
                     /translation="MHKVVLLRHGESVWNMENRFTGWTDVDLTQRGVEEARRAGLMLK
                     EAGFAFDCAYTSVLKRAIKTLWLALETMDLMWIPVSHSWRLNERHYGALQGLNKAEMT
                     AKFGEEQVLLWRRSYDLPPPALTRDDPRHPRGDPRYAGLAESELPLTECLKDTVERFL
                     PYWKDAIAPAVRQGKRVIIVAHGNSLRALIKYLDGVSDQKIVGLNVPTGRPLVYELDA
                     GLKPVRSYYLGDQAEIEAAMRAVADQAKVKP"
     gene            1580..>2457
                     /locus_tag="VEM33_07915"
     CDS             1580..>2457
                     /locus_tag="VEM33_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51791.1"
                     /translation="MNPPRRALKGIAFPALLRGMRCLFCAVLPLIPASALAQSEAEKL
                     DALRTRIEGLRAKIAEAEESRTEARDQLRDSERSISEASRALRALGGRRAAAQRDLDT
                     LLRRSQVLETEIASRREGLGRLLTLRYLNGEQSGLKLLLSGGEPGRIARELHYYGYVS
                     RAQAEFIGELRSSLARLKELEARTRERNIEIAAIESARRTERAALIKQQEEHRKVLAR
                     ISSQLRTQRREVQQLERDESRLSRLVEELGRVIAATPLLRNERVPEPDGSEGSLSGLK
                     RGLRLPIRGVLANRFGA"
BASE COUNT          441 a          810 c          782 g          424 t
ORIGIN      
        1 tcgctcatca ggactccgat agaattgaaa aggcgcgcgg aggctagtcc gcgagcagcg
       61 agtcgagctt tcctgcgtgt tccagcgccg cgagctcttc gaatccaccc acgtgcgtat
      121 caccgatgta gatttgcggc accgtgcgcc tgccggtcct gttcatcatt tcctcgcgtt
      181 ggcgcggatc gagatcgacc cgtattttct cgatctcggt tacgcccttc gaacccagca
      241 gcatctccgc gcgcaggcaa aacgggcaga cggcggtcga gtacatccga atcttggcca
      301 cttacttctc cagaggcagg cccgcttggc gccaggcccg gatcccgccg ctcagcgtga
      361 aaacctgctc gaagccctgc ttgcgcagcg ctgtcgcggc cttgcccgag cggttgccgt
      421 cgtcgcaaac gacgatcacc ggcttcgcct tgtgcttctc gagctctcct gcgcgcggct
      481 cgagctcccc gagggggatg ttgcgagcgt tcagcaggtg ccctttctcg tactcggccg
      541 catcacgcac gtccagcacg agcgcgttct gctggttgat cagcaaggtc gcctcaagcg
      601 tcgaaaccga ggcgccggag gctccgcggc gcaccgcggg ccaaaccagc atcgcaccgc
      661 tcacgaacgc caccccgacg agaaatatgt tgtcggtgac gaatttcaga atgttgactt
      721 cggccacgtg tcccggggtt ggcgctgcgc gctctgtgct cgaatttcca tgggcgctca
      781 ttataaaatg ctttcttgcg gcgtgaacgg gcgccgcgtc gagaatgccc actatgcaca
      841 aagtcgttct gctgcgccac ggtgaatcgg tctggaacat ggagaaccgc ttcaccggct
      901 ggaccgacgt ggacctcacg caacgtggcg tggaggaagc aaggcgggcc ggcctgatgc
      961 tcaaggaggc gggcttcgcc ttcgattgcg cctacacttc ggtgttgaag cgcgccatca
     1021 agaccctgtg gctcgcgctg gagacgatgg acctgatgtg gatcccggtg agtcattcgt
     1081 ggcggctgaa cgaacgccac tatggcgcgc tccaggggct gaacaaggcc gaaatgaccg
     1141 cgaagttcgg cgaggaacag gtccttctct ggcgtcgcag ctatgacctg ccccctcccg
     1201 cgctcacgcg tgacgacccc cgtcatccgc ggggtgaccc gcgctacgcc ggtctcgccg
     1261 aatccgagct gccgctcacc gagtgcctga aggacacggt ggagcgtttc ctgccctatt
     1321 ggaaagacgc gatcgcgccg gcggtgcgcc agggcaagcg ggtcatcatt gtcgcgcatg
     1381 gcaacagcct gcgtgccctc atcaaatatc tcgacggcgt cagcgaccag aaaatcgtcg
     1441 gactcaacgt gcccaccggc cggccgctcg tctacgagct cgatgcgggc ctcaagccgg
     1501 tgcgcagcta ttatctcggc gaccaggccg agatcgaggc ggcgatgcgt gccgttgccg
     1561 atcaagccaa ggtgaagcct tgaatccacc gcggagggcg ctcaagggaa tcgccttccc
     1621 tgcgcttctc cgcggcatgc gctgcttgtt ttgcgcagtg ctgccgctca tcccggcctc
     1681 ggcgctcgcc cagagcgagg cggaaaagct cgacgctctc cgaacccgca tcgaagggct
     1741 gcgcgcgaaa atcgctgaag ccgaggaaag ccgcaccgaa gcgcgcgacc agctgcgcga
     1801 ctccgagcgc tccatatccg aagcgagccg cgcgcttcgc gctttggggg gcagacgcgc
     1861 agcggcgcag cgcgacctcg atacgctgct gcggcgtagc caggtgctcg agacggaaat
     1921 agcctcgcgc cgggaaggcc tcgggaggct gctcaccctg cgttatctca atggagagca
     1981 aagcgggctg aaacttctgc tctcgggcgg ggagcccggc cgcatcgcgc gcgagctgca
     2041 ttactacggc tatgtttccc gcgcacaagc cgagttcatc ggcgagctgc gctccagcct
     2101 cgcccggctg aaagagctcg aggcgcggac ccgggaaagg aacatcgaaa tcgcggccat
     2161 cgaatcggcg cggcgcaccg agcgcgccgc gctgatcaaa cagcaggagg agcaccgcaa
     2221 agtgctcgcg cgcatctcca gccagttgcg cacgcagcgc cgcgaggtgc agcaactcga
     2281 gcgtgacgag tcgcggttgt cgcggctcgt cgaggagctc ggcagggtga tcgcggccac
     2341 gccgctcctt cgcaacgaac gagttcccga gcctgacggt tcggaagggt ccttatcggg
     2401 gctcaagcgc ggactacggc tgccgatcag gggtgtactc gcaaaccgct tcggcgc
//
LOCUS       DASPZD010000409         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1368372, whole genome shotgun sequence.
ACCESSION   DASPZD010000409 DASPZD010000000
VERSION     DASPZD010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368372"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VEM33_07920"
     CDS             <1..308
                     /locus_tag="VEM33_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYS51792.1"
                     /translation="STHTHRETIGNETLNFVDGVPFVIANLPTSVDLLKMDCEGCEYP
                     MFKDERFLDHLTPAEIVVEYHQGETRLVEILKRRAYTLEIVPCGKTFGYIYAKRAPA"
     gene            complement(284..1330)
                     /locus_tag="VEM33_07925"
     CDS             complement(284..1330)
                     /locus_tag="VEM33_07925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYS51793.1"
                     /translation="MSAGSPSFLFINVARIGDTLLATPAMRAIATAHPGCRITALAHP
                     KRAEVLRGLPFVARVGGITKNTAPWRGLLGGRRYDYALVYGFDEALVAYALRVAGSVV
                     AFRQRNEALNRRLYRCVDEAPAGTENAVARCLRLTTALGIPAAGRRLAYRVTQHEAAA
                     AHARLAAEVSAEASPLIGMNVATFPTKVFRRWPVAQFGELAEGIIAERPRAHVLIFGG
                     REEVEDARRLKDQLRDRAVLYAGRLSLRESAAMMSLTDLYVGLDTGPTHLMSAFDIPL
                     VALYHCRIPSRQIAPLDHPCLYALDHPRQGRDCAESTPMSEITVDAVLAQVRRALAEH
                     PPRRPSSQAGARFA"
     gene            complement(1359..2102)
                     /locus_tag="VEM33_07930"
     CDS             complement(1359..2102)
                     /locus_tag="VEM33_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51794.1"
                     /translation="MSIDPARINIAATEALSAFQSRVAGIPYERKGILYSEMFFLYLC
                     AQAAKPSRIFESGRARGQSTLVFSVIFPDLDVVSIESDPNSPDVQVAQERLRGRDNVS
                     LRFGDATRILPAEVRKGDIVLIDGPKGFRGLRLALRLLATGRVTQVFIHDCGLGTEER
                     AFLDAHLRGALYSDSIEFARVARVLDAACWADMPPDRRFDGERPPHAYGYGLACIPFD
                     PAVSYRMLFARAVVTGFKSRIKRGAGDHG"
     gene            2180..2947
                     /locus_tag="VEM33_07935"
     CDS             2180..2947
                     /locus_tag="VEM33_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYS51795.1"
                     /translation="MLSRHTLKWLLRSRSLQLTRDRDPSRYVPGAVSSFRWRGRPLHY
                     RPGSSDPEVIYKILLRRGTKAEYHIPEELNPSCVWDIGANIGAASLYFSDRFPQAEIH
                     CFEPIPENYAMVVRNIEGLDRIRPHSFALGAKGGTFEIRPSDSALNLGGFSFHDAGTA
                     KKRGQQVNVRTPRAIIEDGTAPPPELIKIDVEGAEYDILAAFEPRVLERVRWITGELH
                     GNRSFELLEFLSRWFDIETRKTLGKRLFNFLARNRSV"
     gene            2992..>3558
                     /locus_tag="VEM33_07940"
     CDS             2992..>3558
                     /locus_tag="VEM33_07940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYS51796.1"
                     /translation="MNRNLSSATGNEAERPRILVIRRDNIGDLVCTTPLIFALRGRFP
                     RAHIAALVNTYNEAVLERNPAVDAIYAYEKGKHRGKGRSVLSVHLDRLRLIMKLRKER
                     FNYAILATPGFEPRSMRFARLIGARYVLGYVETGRTQSRPDIALPYDRGATGHEVERV
                     FGLLKAFGVNGPPPSARVFPDVEARRSIE"
BASE COUNT          678 a         1129 c         1142 g          609 t
ORIGIN      
        1 tttccacgca tacgcacagg gaaactattg gcaacgaaac actgaatttc gtcgacggag
       61 tgcccttcgt gatcgccaac cttccgactt cggtcgatct gctcaagatg gactgcgaag
      121 gctgcgagta tccgatgttc aaggacgaac gcttcctcga tcacctgacg cctgccgaga
      181 ttgtcgtgga gtatcaccaa ggcgagacgc gtctggtcga gatcctgaag cgccgcgcct
      241 acacgctcga gatcgtgccc tgcgggaaaa cgttcggcta catctacgcg aagcgagcgc
      301 cggcctgaga gctcgggcgc ctgggcggat gctcggcgag cgcgcgccgc acctgcgcca
      361 gcactgcatc gacggtgatt tcggacatcg gcgtcgattc cgcgcaatcc cggccctgcc
      421 ggggatggtc gagcgcgtac aggcagggat ggtccagggg cgcaatctgg cggctcggga
      481 tccggcaatg gtagagcgcc accagcggaa tgtcgaaggc gctcatgaga tgagtcggac
      541 cggtatccaa gcctacatag agatcggtca ggctcatcat cgcagcgctc tcgcgcaggc
      601 tcaggcgccc ggcgtagagc acggcccgat cgcggagctg atccttcagg cggcgcgcat
      661 cttcgacctc ttcgcgtccg ccgaaaatca gtacatgcgc gcgcggccgt tccgcgatga
      721 tgccctcggc caattcgccg aactgcgcca ccggccagcg gcggaaaact ttcgtcggga
      781 acgtcgcgac gttcatgccg atcaggggcg acgcttccgc agaaacctcg gctgcgaggc
      841 gcgcgtgagc ggcggcggcc tcatgctgtg tcactcgata ggcgagccgt cgcccggcag
      901 ccgggatccc gagggccgtc gtcagccgca ggcagcgcgc aaccgcgttc tcggtccccg
      961 cgggcgcctc gtccacgcag cgataaagcc gccggttgag tgcttcgttg cgctgccgga
     1021 aggcgacgac ggacccggcc acgcgcaggg cgtaggcgac gagcgcctcg tcgaagccgt
     1081 acacgagggc gtaatcgtag cgccggcccc cgagcagtcc gcgccagggc gcggtgttct
     1141 tggtgatgcc tcccacgcgc gctacgaacg gcagcccgcg cagcacctcc gcccgcttgg
     1201 gatgggcgag cgcggtgatc cggcagccgg ggtgggccgt ggcgatcgcc cgcatggctg
     1261 gcgtcgcgag cagcgtgtcg ccgatgcgcg ccacgttgat gaaaagaaac gaggggctgc
     1321 cggcagacat tcaatcgccg gcttcgcaat gcacggcctc acccatgatc cccggctccg
     1381 cgcttgatac gggacttgaa tccggtgacc acagcccgcg cgaacagcat tcgataggag
     1441 accgccgggt cgaatggaat gcatgccagg ccgtaaccgt atgcgtgtgg cggccgctcg
     1501 ccgtcgaagc ggcgatccgg tggcatgtcc gcccagcagg cggcatcgag cacgcgcgcg
     1561 acgcgcgcga attcgatcga atcgctgtac aacgcgccgc gaagatgggc gtcaagaaaa
     1621 gcgcgctcct cggttcccaa tccgcagtcg tgaatgaaaa cctgcgtcac ccttccggtc
     1681 gcgaggaggc gcagggccag gcgcagaccg cgaaaacctt tcggcccgtc gatgagaaca
     1741 atgtcccctt ttcgcacttc ggcgggaaga atccgggttg cgtcgccgaa ccgcagcgat
     1801 acgttatcgc gcccgcgcaa gcgctcctgc gcaacttgaa cgtcgggaga attcggatcg
     1861 gactcgatgc tcacaacgtc cagatctgga aaaataacgg agaacactaa ggtgctctgg
     1921 ccgcgtgcgc gtccggactc gaaaatgcgg ctgggtttgg ccgcttgcgc acacaaatag
     1981 agaaagaaca tctccgaata gagtatgcct ttccgctcgt acggaatgcc ggcgacgcga
     2041 ctttggaacg ccgacaacgc ctccgtggcg gcaatattga tgcgggcggg atcgatgctc
     2101 attgaggccg cggagcgtag gcccgaatgt taccgtaagc gtgcagatcc gcggtaacat
     2161 ccggcagaag gagcatgtcg tgctcagtag gcacactcta aaatggttgc tgcgctcgcg
     2221 atcgctgcag cttacacgcg accgcgatcc gtcacgttac gtcccgggcg ccgtatccag
     2281 cttccgctgg cgcggccgcc cgttgcatta ccggcccggc agcagcgatc cagaggtgat
     2341 ttacaagatc ctgttgaggc ggggaaccaa ggcggagtac cacatcccgg aagagcttaa
     2401 ccccagctgt gtgtgggata tcggtgccaa catcggcgcg gcatcgcttt atttctccga
     2461 ccgctttcct caggccgaga ttcattgctt cgagccgatt ccggaaaact acgcgatggt
     2521 cgtacgcaac atcgaggggc tcgaccgaat tcggccgcac agcttcgccc tgggagcgaa
     2581 gggaggaaca ttcgagattc gaccctcgga ttcggccctc aatctcggcg gattttcgtt
     2641 ccacgacgcc gggacggcca agaaacgggg gcaacaggtg aacgtgcgca caccgcgggc
     2701 gattatcgag gacggcacgg cgccgccacc ggagctcatc aagatcgacg tcgagggggc
     2761 cgagtacgat atcctcgcag ccttcgagcc gcgggtgctc gagcgcgtgc gctggatcac
     2821 gggtgagctt cacggcaacc ggtctttcga attgctggag ttcctctcgc ggtggttcga
     2881 tatagaaacg aggaagacgc tgggcaagcg actgttcaat ttccttgcga gaaaccgtag
     2941 cgtctgagct gctggtcgag ccgcgacgcg gacggcactc gtcgaacgct catgaatcgt
     3001 aacctatcct ccgcgacagg aaacgaggcg gagcgtccgc gcattctggt gattcgccgc
     3061 gataacatcg gcgatctcgt ttgcacgacc ccgctcatat tcgcgctgcg cgggcgcttt
     3121 ccgcgcgccc atatcgcggc gctggtcaac acttacaacg aggcggttct cgagcggaat
     3181 cccgctgtcg acgccatcta cgcctacgaa aagggaaagc accgcgggaa ggggcgctcc
     3241 gtcctttcgg tccacctcga tcggctgcgc ctgatcatga aactgcgaaa ggagcgcttc
     3301 aactacgcga tcctcgcgac cccgggattc gagccccgtt ccatgcgatt cgcccggctc
     3361 atcggggcgc ggtacgtgct cggttacgtc gagaccggca ggactcaatc caggccggac
     3421 atcgcgctgc cttacgaccg cggcgccacc gggcacgagg tggagcgcgt gttcggcttg
     3481 ctcaaggctt tcggcgtcaa cggtccgccg ccttccgctc gggtgtttcc cgatgtagag
     3541 gcgcgtcgca gcatagag
//
LOCUS       DASPZD010000410         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1125455, whole genome shotgun sequence.
ACCESSION   DASPZD010000410 DASPZD010000000
VERSION     DASPZD010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125455"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VEM33_07945"
     CDS             complement(<1..375)
                     /locus_tag="VEM33_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYS51797.1"
                     /translation="MLSGSIVAIVTPMQDDGRLDFERFKSLIDFHIEQGSDGIVVVGT
                     TGESPTVNFDEHKELIRLAVAHARGRIPIVAGTGGNSTVEAIELTQSAKNAGADACLS
                     VVPYYNKPTQEGMYRHFRTIAEK"
     gene            complement(403..987)
                     /locus_tag="VEM33_07950"
     CDS             complement(403..987)
                     /locus_tag="VEM33_07950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYS51798.1"
                     /translation="MSSCAHGPEAPSAWVCRWAALIRPGGRVLDVACGQGRHLRYLQS
                     LGFAVVGVDRDAQALSAVGELNGAEVCVADIEAGPWPFAPGGFDGVVVTNYLHRPLFP
                     DLIGALRPGGVLIYETFALGNERYGRPSNPAFLLRPAELLHSVEPLAVVAFEQGLVSV
                     PKQAVIQRVCAVREGRGAMTLEALPRQPVTRRLR"
     gene            1034..2254
                     /locus_tag="VEM33_07955"
     CDS             1034..2254
                     /locus_tag="VEM33_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYS51799.1"
                     /translation="MAPSSRPVTFPGAFGTSLAARLDAPAGPPLAYAIFAHCFTCSKE
                     SKAAAAISAALAEKEFAVLRFDFTGLGGSEGDFANTNFSSNVDDLVAAADFLRREHRA
                     PTLLVGHSLGGTAVLAAASRIPEAGAVATIGSPFDPDHVLGLIKDSVEKIDAEGDARV
                     GIAGRDFRIKKQFLDDIRAQKIRAAVERLGKALMVMHSPRDAIVNIDNAARIFTAAKH
                     PKSYVSLDPADHLLLRREDALYAGEVLAVWASRYVAPSPEAAPALPAGTVLVRETREG
                     KFTNQVFAGRHVIHADEPLEAGGLDTGLSPYDLLCASLGACTAMTLRSYADMKGIALE
                     RVSVELKHEKIHAADCAECETREGKIDRIDRLITLEGDLGPQQRAKLLEIADKCPVHR
                     TLHSEVLIKTQLAA"
     gene            complement(2251..2793)
                     /locus_tag="VEM33_07960"
     CDS             complement(2251..2793)
                     /locus_tag="VEM33_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HYS51800.1"
                     /translation="MLDFWFGAPDSRERGRPRKSWFQKSEPFDAEIRRRFLAAWERAA
                     RGELGPWQTTPLASIALVVTLDQFPRNMFRGTARAFASDPLALAAARSLIAAGFDRLL
                     SPVERLFACLPFTHAEDLAAQRRSLALSHGLDPEEVRSAKRHYEIIARFGRFPHRNAV
                     LGRSSTPEETEFLKQPGSSF"
BASE COUNT          505 a          960 c          950 g          443 t
ORIGIN      
        1 tttctccgcg atcgtgcgga aatgccggta cataccctcc tgcgtgggct tgttgtaata
       61 gggcacgacc gagagacagg cgtcggcgcc ggcgttcttc gccgactgcg tcagctcgat
      121 cgcctcgacg gtggaattgc cgccggtgcc ggccacgatt gggatgcgcc cgcgcgcgtg
      181 ggcgaccgcg agccggatca gttccttgtg ctcgtcgaag ttgacggtgg gcgattcccc
      241 ggtagtgccg acgacgacga tgccgtcgct gccctgctcg atgtggaaat cgatgagcga
      301 cttgaagcgc tcgaaatcga gccgcccgtc gtcctgcatt ggcgtaacga tcgcgactat
      361 cgacccggaa agcatggggc ctcctgattt aagcgcttca ttctaacgca atcgccttgt
      421 caccggctgc cgcggcaggg cttcgagcgt catcgcgccc ctgccttccc gcacggcgca
      481 gacgcgctgg atcaccgcct gcttcggcac ggaaaccagc ccctgttcga acgcgactac
      541 ggccaagggt tccacgctgt gcaagagctc ggcaggccgc agcaggaatg ccggattgct
      601 cggccgcccg tagcgctcgt tgcccaaggc gaaagtctcg tagatcagca ccccgccggg
      661 ccgcagcgcg ccgatcagat cgggaaagag cggccgatgc agatagttgg tcacgacgac
      721 gccgtcgaat ccgccgggcg cgaagggcca gggtcccgct tcgatgtccg cgacgcagac
      781 ctccgccccg ttcagctcgc ccacggccga aagcgcctgc gcgtcgcgat cgactccgac
      841 caccgcgaag cccagcgact ggaggtagcg caggtgacgg ccctgaccgc aggcgacgtc
      901 gagcacgcgc ccgcctggcc ggatcaacgc ggcccagcga catacccagg cagacggcgc
      961 ttcgggcccg tgcgcacacg acgacatatc gcgtctatta tccccgtgac gcaacagcta
     1021 cgcaggagca catatggcac ccagcagtcg acccgtgact tttcccggcg cattcggtac
     1081 gtcgctcgcc gcgcggctgg acgcgcccgc cgggccgccg ctcgcctacg ccatcttcgc
     1141 ccactgcttc acctgctcca aggagagcaa ggccgcggcc gcgatcagcg ccgcgctcgc
     1201 cgagaaggag tttgccgtgc tgcgcttcga tttcaccgga ctgggcggga gcgagggcga
     1261 tttcgcgaac acgaatttct cctcgaacgt cgacgatctc gtcgccgcgg cggacttcct
     1321 caggcgcgag caccgcgccc caacgctcct ggtcggacac agcctcggcg gcacggcggt
     1381 gctcgctgcg gcttcgcgga tccccgaggc gggcgcggtc gcgaccatcg gatcgccatt
     1441 cgacccggac cacgtcctgg gcttgatcaa ggactcggtc gaaaaaatcg acgcggaggg
     1501 cgacgcgcgg gtcggtatcg ccggccgtga tttccggatc aagaagcagt ttctcgacga
     1561 catccgggcg caaaaaatcc gcgccgcggt ggagcgtctc ggcaaggcgc tgatggtgat
     1621 gcattctccg cgcgacgcca tcgtaaacat cgacaatgcg gccaggatct tcaccgcggc
     1681 caaacatccg aagagctacg tatcgctcga tccggccgac cacctcctct tgcgccgcga
     1741 agacgcgctc tacgcgggcg aggtgctcgc cgtctgggct tcgcgttacg tcgccccgtc
     1801 gcccgaggcg gcgccggcgc tgccggcagg cacggtgctc gtgcgcgaga ctcgcgaggg
     1861 caagttcacc aaccaggtct tcgccggccg gcacgtaatc cacgcggacg agccgctcga
     1921 ggcgggaggc ctggatacgg gcttgtcgcc ctacgatctg ctgtgcgcgt ccctgggagc
     1981 gtgcacggcg atgacgctgc ggtcgtacgc cgacatgaag ggcatcgcgc tcgagcgcgt
     2041 cagcgtggag ctcaagcacg agaagatcca cgcggccgac tgcgcggaat gcgagacccg
     2101 cgaggggaag atcgaccgaa tcgacaggtt gatcacgctc gagggtgatc tcggcccgca
     2161 gcagcgcgcg aagctcttgg agatcgccga caagtgcccg gtccatcgga ccttgcactc
     2221 cgaagtcctg atcaagaccc agctcgcggc ctaaaacgaa gacccgggct gcttcaggaa
     2281 ttcggtttcc tcgggcgtcg acgagcggcc gagaacggcg ttgcggtgag gaaagcgccc
     2341 gaagcgcgcg atgatctcgt agtgccgttt cgcgcttcgc acctcctccg ggtcgaggcc
     2401 gtgggacaag gcgagagagc ggcgttgtgc ggcgagatcc tccgcgtgtg tgaagggcag
     2461 gcaggcgaac aagcgttcga ccggggacag cagccggtcg aatcccgcgg cgatcaggct
     2521 cctcgccgcg gcgagcgcca ggggatcgct cgcgaaggcg cgcgccgtgc cgcggaacat
     2581 gttgcgcgga aactgatcga gcgtcacgac gagggcgatc gatgcgagcg gcgtcgtttg
     2641 ccagggtccg agctcgccgc gcgccgcccg ttcccaggcg gcgagaaagc ggcggcggat
     2701 ttcagcgtcg aacggttccg acttctggaa ccacgatttg cgcggccgac cgcgctcacg
     2761 cgaatccggc gcgccgaacc agaaatcgag cacctcgatg aagtcgagaa tcagttgcag
     2821 agccgcaagt tggcgaaagc gatcagcatc gccggatt
//
LOCUS       DASPZD010000411         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_528408, whole genome shotgun sequence.
ACCESSION   DASPZD010000411 DASPZD010000000
VERSION     DASPZD010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528408"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VEM33_07965"
     CDS             <1..285
                     /locus_tag="VEM33_07965"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HYS51801.1"
                     /translation="DYSYSNIRDVFDQQTLVGAPVATIPDVNTKLTRLNVFGRYAIDK
                     ASGVRLDYIYDRYSTNDWLWSTWTYTDGTTLSENQKQTVNFFAASYYFKF"
     gene            336..614
                     /locus_tag="VEM33_07970"
     CDS             336..614
                     /locus_tag="VEM33_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYS51802.1"
                     /translation="MKAIRFVVAVGCALAAGSVFAQSGADVVKAKGCLNCHAVDQKKV
                     GPSFKDIAAKKGDQAALVAKLKDGKGHPKVAASDAELKAAVGYVMSTK"
     gene            624..1175
                     /locus_tag="VEM33_07975"
     CDS             624..1175
                     /locus_tag="VEM33_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HYS51803.1"
                     /translation="MADMQPGGGWWSRLFSPSARYSVFTLLVAGIVLGIAGLLSFDYV
                     LHATSTNEFCSSCHQNDAAKEWRESKHYNNRVGFVAGCSDCHLPREFVPKMVRKVKAA
                     KEVWGHFTGVINTPEKYEAHRKEMAENEWARMKGNGAKECRNCHSPAQSTDKDKAEMH
                     KGALSGGAICIDCHKGVAHKAPG"
     gene            complement(1172..2116)
                     /locus_tag="VEM33_07980"
     CDS             complement(1172..2116)
                     /locus_tag="VEM33_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYS51804.1"
                     /translation="MRLLTFRLRGVEAPRIGARVSRQVLDLAAAAGVAGDAPPPSRMR
                     DLLAAGKPAMKQVRKLFEQAQADRDGFGAALLDEREIRFLPPIPDADKFLCVGKNYRT
                     HLEELKRNDLIAETPQEPTAFVKLNSCLAGHNAKVARPDGVVALDYEPELVFVIGKRA
                     LAAKKKDALDYVAGVTILNDLTDRDAQKREVASGSRFWTGKNIPGFGPLGPEIVTMDE
                     IPDPYDLWMTCSVNGEQRMRVNTRDQIWKLPDILEHFSRHIPIEPGDMFSTGAPGGVA
                     VGKANASELFLKPSDVVECSIEGVATLRTYIVSPGAAR"
     gene            complement(2150..2617)
                     /locus_tag="VEM33_07985"
     CDS             complement(2150..2617)
                     /locus_tag="VEM33_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51805.1"
                     /translation="MPLSHIEHYLVLSDEIDATRDWYRDVLGMQEGWHPDFGFPVYWM
                     YLAGVDVVHIGRSAKHAGENQKAYLGRLAQDTGAGTGAIDHIAFRAKGLKETMAHLRR
                     HKVEFKERRANGQALYQLFMYDPNGIKVELNFDAAEAQGIEPEITAEKLAARK"
     gene            complement(2628..>2838)
                     /locus_tag="VEM33_07990"
     CDS             complement(2628..>2838)
                     /locus_tag="VEM33_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51806.1"
                     /translation="LQKLLETRPAKLVVLILDITLIDVFLFFAGVALLDLSQSFSIFS
                     LWSAMLFSFLLVYMVMVSHKDIKKY"
BASE COUNT          510 a          888 c          947 g          493 t
ORIGIN      
        1 gattacagct attcgaacat ccgggatgtg ttcgaccagc agaccctggt cggcgctccg
       61 gtcgcgacga tcccggacgt gaacaccaag ctgacccggc tcaacgtgtt cgggaggtac
      121 gccatcgaca aggcgtcggg cgtccgcctc gactacatct acgaccgcta cagcaccaat
      181 gactggctct ggtcgacctg gacgtacacc gacggcacga ccctgagcga gaaccagaag
      241 cagacggtga acttcttcgc ggcgtcgtac tattttaagt tctaggaatg cggtaggatt
      301 tgcagcgctt tcccaacttt cgaacgaggc actcaatgaa ggcaatacga ttcgtcgttg
      361 cagtcggctg cgccctggcg gccgggtcgg tgttcgccca gtcaggcgcg gacgtggtca
      421 aggccaaggg ctgtctgaac tgccatgcgg tggaccagaa gaaggtcggt ccctcgttca
      481 aggacatcgc cgcgaagaag ggcgaccaag ccgccctcgt cgccaagctc aaggacggca
      541 agggtcaccc gaaggtcgcg gcctcggacg ccgagctcaa ggcggcggtc gggtacgtga
      601 tgtccacgaa gtagccagcc agcatggccg atatgcagcc aggcggcggc tggtggtcgc
      661 gtttgttctc gccgagcgcg cgctattcgg tcttcacgct gctcgtcgcg ggcatcgttc
      721 tgggcatcgc aggcctcctg tccttcgact acgtcctgca cgccaccagc acgaacgagt
      781 tctgcagcag ctgccatcag aacgacgcgg cgaaggagtg gcgcgaatcg aagcactaca
      841 acaaccgcgt cggcttcgtc gccggctgct ccgactgcca cctgccgcgc gagttcgtgc
      901 ccaagatggt gcgcaaggtc aaggcggcga aggaggtctg gggccacttc accggtgtca
      961 tcaatacgcc ggagaaatac gaggcgcacc gcaaggagat ggccgagaac gagtgggcgc
     1021 gcatgaaagg caatggcgcc aaggagtgcc gcaactgcca cagccccgcg caaagcaccg
     1081 acaaggacaa ggcggagatg cacaagggcg cgctgtcggg cggggcgatc tgcatcgact
     1141 gccacaaggg cgtcgcgcac aaagcgcccg gctagcgcgc cgcgccgggt gagacgatgt
     1201 aggtgcgcag ggtcgcgacg ccttcgatcg agcattcgac cacgtcgctc ggcttgagga
     1261 agagctccga ggcgttggcc ttgcccacgg cgacgccgcc gggcgccccg gtcgagaaca
     1321 tgtcgccggg ctcgatcggg atgtgccgtg agaagtgctc gaggatgtcg ggaagcttcc
     1381 agatctggtc gcgcgtgttg acgcgcatgc gctgctcgcc gttcaccgag caagtcatcc
     1441 acagatcgta gggatcgggg atctcgtcca tcgtgacgat ctccggccca aggggcccga
     1501 agcccgggat gttcttcccg gtccagaagc gcgagcccga ggcgacttcg cgcttctgag
     1561 cgtcgcggtc ggtgaggtcg ttcaggatgg tgacccctgc gacgtagtcg agcgcgtcct
     1621 tcttcttcgc tgccaaggcg cgcttgccga tgacgaatac cagctcgggc tcgtagtcga
     1681 gcgcgacgac cccgtcgggc cgagcgacct tggcgttgtg gcccgcgagg caggagttga
     1741 gcttgacgaa ggcggtgggc tcctgcggcg tctcggcgat caggtcgttc cgtttcagct
     1801 cttcgaggtg cgtgcggtag ttcttgccga cgcacaggaa cttgtcggcg tcggggatgg
     1861 gcggcaggaa gcggatctcg cgctcgtcga gcagcgcagc gccgaaaccg tcgcggtcgg
     1921 cctgcgcctg ctcgaacagt ttgcgcacct gcttcatcgc cggcttgccg gcggcgagca
     1981 ggtccctcat gcgcgaaggc ggcggtgcgt cgccggcgac tcccgccgcg gcggcgagat
     2041 cgagcacctg gcgggaaacc cgcgcgccga tccgtggggc ttccaccccg cgcagcctga
     2101 acgtaaggag tcgcatgccg tactcccgat gcggcggaat ctctcggagc tatttccgag
     2161 ccgccagctt ctcagcggtt atttcgggct cgatcccctg cgcttcggcg gcgtcgaagt
     2221 tcagctcgac cttgatgccg ttggggtcgt acatgaacag ctggtagagc gcctggccgt
     2281 tcgcgcggcg ctccttgaat tcgaccttgt ggcggcgcag gtgcgccatg gtctccttca
     2341 gccccttcgc gcgaaacgcg atgtggtcga tcgcgcccgt gcctgcaccg gtgtcctgcg
     2401 cgaggcgccc caggtaggcc ttctggttct cgcccgcgtg cttcgcgctc ctgccgatat
     2461 ggaccacgtc cacgcccgcg aggtacatcc agtagacggg aaacccgaag tccgggtgcc
     2521 agccctcctg catgccgagc acgtcgcggt accagtcgcg cgtcgcgtcg atctcgtcgg
     2581 aaaggacgag gtagtgctcg atgtgggaga ggggcatgcc gggcccgcta gtatttcttg
     2641 atgtccttgt gcgagaccat caccatgtag acgagcagga acgagaacag catcgcgctc
     2701 cacagcgaga agatcgagaa gctttggctc aagtcgagca gcgccacgcc cgcaaaaaag
     2761 aggaatacgt cgatcagcgt gatgtcgaga atcagcacca cgagcttcgc cgggcgggtt
     2821 tcgagcagtt tctgcaag
//
LOCUS       DASPZD010000412         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_448809, whole genome shotgun sequence.
ACCESSION   DASPZD010000412 DASPZD010000000
VERSION     DASPZD010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448809"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VEM33_07995"
     CDS             <1..1080
                     /locus_tag="VEM33_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51807.1"
                     /translation="AGIILRALGVSLSKLGDGHTAATATASPFRVGQSFVLHLAALDL
                     YVGVLPFAAMAIMVRLAFKNDALRELRVICALTISLSCWLAAVAAKYLVADYPGSFPR
                     IYDRYMFYVVPLLLIVFLGWIREGLPRPRRTAIIAAVGSAALPALIPYAHVLSGGEWG
                     VSSSSVALLPWANIRFILGTPLAVYPVLVLGGSYLAWVFARSENRDWLLFLVAANFVV
                     LNVFALSGNSAVADQALRQGVGSTRDRSWIDEAVGSNARVDALWAGVDRAGISARYGI
                     WESDFFNQSVRRFYYLREGLHRDYPGDHLVVSHRNLYLPDGKEFRARYVLTDVKTPLQ
                     ATKVAVNRAAGMLLYRVDGPVRLLR"
     gene            complement(1108..1809)
                     /locus_tag="VEM33_08000"
     CDS             complement(1108..1809)
                     /locus_tag="VEM33_08000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYS51808.1"
                     /translation="MVPAPPAGFETELPGGGRIFLHHRADIGLVVLMSGNFECAETTC
                     ARELAREGTVAIDVGANVGMFTIPLALAVGDRGRVLAIEPWPENVAMLNYNLRLNALR
                     NVVVRDIAASAECGEVLLHLGSDPAFHSTTAVVKGREAGENLRVPSQTLDAIWEDSGT
                     PEVSFLKIDTEGGEFEILEGASRLLRTWRPAILLEAKGERRAKELDQRLLPQGYVRTR
                     RRGFAPGNFLYAASE"
     gene            complement(2699..>2886)
                     /locus_tag="VEM33_08005"
     CDS             complement(2699..>2886)
                     /locus_tag="VEM33_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51809.1"
                     /translation="AIDELVSAGDLEELGSTKTQLDRELSQLASQQQVDKELEQMKAE
                     AGSGGEKKELGEAPPSN"
BASE COUNT          514 a          907 c          875 g          590 t
ORIGIN      
        1 gccggcatca tacttcgggc acttggcgtg tcgctcagca agttggggga cggtcacact
       61 gcggcaactg caaccgccag ccctttccga gtcgggcagt cattcgtgct ccacctggct
      121 gccctagatc tctacgtagg ggtgcttcct tttgcggcga tggcgatcat ggtccgtttg
      181 gcgttcaaga acgacgcgct gcgagagcta cgcgtcatct gtgctctcac gatcagcctc
      241 tcatgctggc ttgctgcggt agcagccaaa tacctggttg ccgactatcc cggctcgttc
      301 ccgcggatct atgaccgcta catgttctac gtggtgcctc tcctccttat cgtctttctc
      361 ggctggatcc gcgagggcct accacgtcct cggaggacgg cgattatcgc cgcggttggt
      421 tcggcggcct tgcccgccct cattccttac gcacacgttc tcagcggcgg ggagtggggc
      481 gtttcctcga gcagtgtggc gctgctcccg tgggcaaata tccgcttcat actcggcacg
      541 cctctcgctg tgtaccccgt gctcgtcctc ggaggctcgt acctcgcgtg ggtctttgct
      601 cggtcggaaa accgggactg gctcctcttt ctcgtcgccg cgaacttcgt cgttctcaat
      661 gtgtttgcgc tgtctgggaa ttcggccgtc gcggaccagg ctctgagaca gggcgtgggc
      721 tcaactcgcg accgttcctg gatcgatgaa gcagttggtt cgaacgcacg cgtggacgct
      781 ctttgggcgg gcgtcgaccg agctggaata tcagcccgat acgggatctg ggaaagcgac
      841 ttctttaacc aaagtgtgcg ccgtttctac tatctccggg aaggtctcca tcgtgactat
      901 ccaggcgacc acctagtcgt cagccatcgg aatttgtacc tccctgatgg aaaggaattc
      961 cgggcccgat acgtcttgac ggatgtgaag actccgctac aggctacaaa ggtcgccgtc
     1021 aaccgagccg cggggatgct cctgtatcgc gtcgatggtc ccgtccgctt gctgcgctga
     1081 aacagcgatc ggggatggcc ccttggccta ttcagaagcg gcgtaaagga agttgccggg
     1141 ggcgaagccg cggcggcgcg tacgaacgta tccctgagga aggagccgct ggtcgagctc
     1201 cttcgcgcgg cgctcaccct ttgcttcgag caatattgcc ggccgccagg tgcgtagtag
     1261 gcgactggcg ccctccagga tttcgaactc accaccctcc gtgtcgatct tcaagaagga
     1321 gacctccggt gtccctgagt cctcccagat cgcgtccagg gtctgagaag gaacccgcaa
     1381 gttttcgcct gcctcccggc ccttgacaac tgcggtcgtg gaatggaaag caggatcaga
     1441 gcctaggtga aggagtactt cgccgcattc tgcagacgcg gcgatatccc gcacaactac
     1501 gttccggaga gcgttgagcc gtaggttgta gttcagcatg gcgacgttct ccggccatgg
     1561 ctcgatcgca agaactcggc cacgatcgcc aacagccaac gcgaggggaa tcgtgaacat
     1621 ccccacgttt gccccgacat cgatcgcgac cgttccctcg cgcgcaagct cacgcgcgca
     1681 ggtcgtctcg gcgcattcga agtttccact catcaacacg acgagcccga tgtcggcacg
     1741 gtgatgcagg aagattcgcc cgcctccggg cagctcagtt tcgaaaccgg ctggaggggc
     1801 cggcaccatt cgcttgatca gtttctcgag gacgagcttc ttgccgagct gcctttgcga
     1861 atgtgaccaa taccaacgaa ctggccacgc taggtgccaa gcggtacgaa gcccaaggtt
     1921 ccgccgattc gccatcgatt gaccccccgg gcgcctgggg acgcgctcag cggctgtcgg
     1981 ggagaccgaa accatctgga ccgtcagcgc tgtcgggacg cgcccgagac ttcgggggcc
     2041 gatttgccga aggtcatagc tggcgcgtgg gagctgcgaa gatctcgccg aggcccacga
     2101 catcgagtag ccgcgccaag aatcctcggc cacggcacgc ctcgcagcga gtacctgcgg
     2161 tccaagccga gccgcaagcg aggccagtgt cgtagcactc gcggcaggaa acatcactca
     2221 gttgaggtga accgaattgc gcgtgctgtt gcgcctgctc taactggggc ttaaggacag
     2281 cgaccacacg tgaatcgtgg tcccgctatc tcgttaactc catggccacg gggatggtcc
     2341 tggcacgacc taagtcctga ggagcggctg tcagaagtga tggcgattac cgccgcgatc
     2401 cggacgcgcg tcatcagccc cgctgagctg atctcagtct ccaacttgcc gaaacggtct
     2461 cagccgtcag aggcggggat gccgtatgtg cagcttgata ccggtctttg gtgccggcta
     2521 gacgctgcgg cggccgcgga cgaagaagag cgcggcgcca atgatgattg cgacgacgat
     2581 caggatgaca tagaacatga gagtcccttt ccctaggaag gcgtcttgtc ctcctaggtc
     2641 tacggcaagc ggaggacagc gtccttcccc tcagggctag ccagacccac accaaccgct
     2701 agttcgaggg cggcgcctct cccagttcct ttttctcgcc gcctgagccg gcctccgcct
     2761 tcatctgctc cagttccttg tcgacctgct gctgggaagc gagctgcgag agctcccgat
     2821 cgagctgcgt cttggtagag ccgagctctt cgaggtcgcc cgcggagacg agctcgtcga
     2881 tcgctg
//
LOCUS       DASPZD010000413         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1251774, whole genome shotgun sequence.
ACCESSION   DASPZD010000413 DASPZD010000000
VERSION     DASPZD010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251774"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VEM33_08010"
     CDS             <1..425
                     /locus_tag="VEM33_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYS51810.1"
                     /translation="PVHETPENVWELMLDLNAKSVINTARAVVPKMLAARRGKIVNIG
                     AVAGLAGKANMGAYSAAKSAVIRLTESMSAELRDKGINVNCVLPTVIDTPQNRADMPG
                     ADPRRWVAPEALADVILFLASDAARAIHGAAIPVTGLV"
     gene            439..1644
                     /gene="pcaF"
                     /locus_tag="VEM33_08015"
     CDS             439..1644
                     /gene="pcaF"
                     /locus_tag="VEM33_08015"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HYS51811.1"
                     /translation="MDEVFICDAVRTPFGRYGGALSAIRTDDLATLPIKALLERNPSV
                     DWAALDDVIYGCVNQAGEDNRNVARMALLLAGLPQTVPGATVNRLCGSSLDAVGTAAR
                     AIKCGETQFMIAGGVESMSRAPFVMPKADSAFSRAAKIEDTTIGWRFVNPLMKSKYGI
                     DSMPETAENVAEEFKVGRADQDAFALRSQQRWATAQAKGFYGSEIVPVSVPGKKGTVT
                     AVDRDEHPRPDATLEALAKLKGVVKPEGTVTAGNASGVNDGACAMILASEAAAKKHKL
                     GLKARVLGMATAGVAPRIMGVGPAPATRKVLERTGLNLAQMDVIELNEAFAAQALAVT
                     RDLRLPDDAAHVNPNGGAIAIGHPLGASGARLVTTAMYQLHATKGRYALCTMCIGVGQ
                     GIALVIERV"
     gene            complement(1650..2435)
                     /locus_tag="VEM33_08020"
     CDS             complement(1650..2435)
                     /locus_tag="VEM33_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYS51812.1"
                     /translation="MALFCDFENVALGVRDAKYAKFDINKVLERLLLKGSIVLKKAYC
                     DWERYKEFKSDMHEASFELIEIPHVRMSGKNSADIRMVVDALDLCYTKSHVDTFVIIS
                     GDSDFSPLVSKLRENDKTVIGVGVKNSSSDLLVANCDEFIFYDDLVREKPGRRPPRKN
                     PQVKPAVSGEAVPKDNEDKTEEALDLVMQTVEALIAERGAEEKIWGSMVKQALKRRNP
                     GFNESYYGFSSFSKLLDEAESRKMLQLERDEKSGGYIVKSVAH"
     gene            2581..3123
                     /locus_tag="VEM33_08025"
     CDS             2581..3123
                     /locus_tag="VEM33_08025"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HYS51813.1"
                     /translation="MHTEVHVHGTVLLKSGVSQTDIELALRHWFDYVDVDGLADARSA
                     HQDEPGIVFDRRRRSLDICWTGYVGRNFQHSLEGAFESLSPYSEEAAAVEVSYYQEDG
                     QDEHSWVFVGPTPESIRDMQRRRMIDDLSALLARHFGDAEIGEVTALVNQMFARRASD
                     APQDRAQVRSGGVSGKKHLH"
BASE COUNT          570 a          978 c         1016 g          582 t
ORIGIN      
        1 acccggtgca cgagactcct gagaacgtct gggaactcat gctcgatctc aacgcgaaat
       61 cagtgatcaa caccgcgcgt gcagtcgtgc cgaaaatgct cgccgcgcgg cgcggaaaaa
      121 tcgtcaacat cggagccgtc gcgggtctcg ccggcaaagc gaacatggga gcctacagcg
      181 cggcgaagag cgcggtgatc cggctgaccg agagcatgtc ggcggaattg cgggacaagg
      241 gtatcaacgt caactgcgtc ttgcccaccg ttatcgacac gccgcagaac cgcgccgata
      301 tgcccggggc cgatccgcgg cgttgggtgg cgccggaggc tctcgccgac gtgatcctgt
      361 ttctggcctc ggacgccgcg cgtgccattc atggggcggc gattcccgtg accggcctcg
      421 tctgatagat tccgagacat ggacgaagtt ttcatctgcg acgccgttcg cacgcccttc
      481 gggcgctacg ggggcgcgct ctctgcgatc cgtaccgacg acctcgcaac ccttccgatc
      541 aaggcgctct tggagcgcaa cccatcggtc gactgggccg cactcgacga cgtgatctac
      601 ggatgcgtca accaggcggg ggaagacaac cgcaacgtgg cgcgcatggc cctgcttctc
      661 gccggattgc cgcagacggt gccgggtgcc accgtgaacc ggttgtgcgg ctcgagcctg
      721 gatgccgtgg gcacggcggc gcgcgcgatc aagtgcgggg aaacccagtt catgatcgcc
      781 ggcggcgtgg aaagcatgtc gcgggcaccg ttcgtgatgc cgaaggctga tagcgcgttc
      841 tcgcgggcgg cgaaaatcga ggatacgacc atcggctggc gtttcgtcaa cccgctcatg
      901 aaatcgaagt acggtatcga ctcgatgccg gagaccgcgg aaaacgtcgc cgaggaattc
      961 aaagtgggac gcgccgacca ggacgcgttt gcgctgcgaa gccagcagcg ctgggcgacg
     1021 gcgcaggcga agggctttta cggcagtgag atcgttccgg tttccgtccc gggcaagaaa
     1081 ggcacggtaa cggcggtcga ccgtgacgaa cacccgcgcc cggacgcgac tctcgaggcg
     1141 cttgcgaagt tgaagggcgt ggtaaagccc gaaggcacgg tcaccgcggg caatgcttcg
     1201 ggcgtgaacg acggtgcctg cgccatgatc ctcgcctcgg aggcggcagc gaagaagcac
     1261 aagctcggcc tcaaggcacg tgtcctcggc atggcgacgg caggcgtcgc tccacgcatc
     1321 atgggcgtgg gtccggcgcc ggccacccga aaagtcctgg agaggacggg gctcaacctc
     1381 gcgcagatgg acgtgatcga gctgaacgag gcgtttgccg cacaagcgct cgcggtgaca
     1441 cgcgacttga ggctccctga cgacgccgcg catgtgaacc ccaacggcgg tgcaatcgcg
     1501 atcggccacc cgctgggcgc atctggcgcg cgcctcgtca ccacggcgat gtaccaattg
     1561 cacgcgacca agggccgcta tgcgctgtgc accatgtgca tcggcgtagg gcaggggatc
     1621 gctctggtca tcgagcgggt ttgaacttgc taatgcgcga cgctcttgac gatgtagccg
     1681 cccgactttt cgtcgcgctc gagctgcagc atcttgcgcg actcggcctc gtcgagcagc
     1741 tttgagaacg aggagaagcc gtagtaggac tcgttgaagc cggggttgcg gcgcttcagc
     1801 gcctgcttca ccatcgagcc ccagatcttc tcttcggcgc cgcgctcggc gatcagcgct
     1861 tcgacggttt gcatcaccag gtccagcgcc tcctcggtct tgtcctcgtt gtccttcggc
     1921 acggcctccc cgctcactgc gggcttcacc tgtgggtttt tcctcggcgg ccggcgcccc
     1981 ggcttttcgc gcaccaggtc gtcgtagaaa atgaactcgt cgcagttcgc caccagcaga
     2041 tcggagctgg agttcttcac tccgaccccg atcacggtct tgtcgttttc gcgcagctta
     2101 gagacgagcg gcgagaaatc ggaatcgccg ctgatgatga cgaatgtgtc gacgtgcgac
     2161 ttggtgtagc aaagatccag cgcatccacc accatgcgga tatcggccga attctttccc
     2221 gacatgcgca cgtgcgggat ctcgatgagt tcgaacgacg cttcgtgcat gtcggatttg
     2281 aactccttgt agcgttccca atcgcaatag gccttcttca gcacgatgct ccccttcagg
     2341 agcaggcgct cgaggacctt gttgatgtcg aactttgcgt actttgcgtc gcgcaccccg
     2401 agcgcgacgt tctcgaagtc gcagaaaagc gccatgttta ctgtgtcgct cgatgcgctc
     2461 atttcgggtc cttggacatg gcggaggcgg tattatccca ttcccccgtc cagactatgg
     2521 ccggaaaggg ttgaagtccc ggctttctcg gactagcatc ggtgtttcgg gggcaggcaa
     2581 atgcacactg aagttcatgt tcacggcacg gtgttgttga agagcggcgt cagccagact
     2641 gacatcgagc ttgcgctcag acactggttc gattacgtcg acgtggatgg cctcgccgat
     2701 gcgcgcagcg cccatcagga cgagcccggc atcgtgttcg acaggcgtcg gcgctcgctc
     2761 gacatctgtt ggaccgggta cgtcgggcgc aatttccagc attcgctcga gggggcgttc
     2821 gaatcgctgt cgccctactc cgaggaggcg gcggcggtcg aggttagcta ctaccaggag
     2881 gacggccaag acgagcacag ctgggtgttc gtcgggccca cgcccgagtc gatccgcgac
     2941 atgcagcggc ggcgcatgat cgacgatctt tcggccctac tcgcgcgtca tttcggcgac
     3001 gccgaaatcg gcgaggtgac cgcgttagtc aatcagatgt tcgcccgccg cgcctccgac
     3061 gcgccgcagg accgggctca ggtccgttcc ggcggtgttt ccggaaaaaa gcacctgcac
     3121 tagaagtctc cgacggaagt gggccc
//
LOCUS       DASPZD010000414         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_538869, whole genome shotgun sequence.
ACCESSION   DASPZD010000414 DASPZD010000000
VERSION     DASPZD010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538869"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VEM33_08030"
     CDS             <1..771
                     /locus_tag="VEM33_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS51814.1"
                     /translation="SAYAYTGGKAFDAKLPAVVFIHGGEQDHSVWALQSRYLAHHGHA
                     VLAVDLPGHGRSGGAPLASIEDMAGWIVASLDAAKVGTAALVGHSMGSLAALECAARH
                     PQRVNRVALLGTAFPMRVSPDLLQATKHRESDAHGMINAWSHSAYAHYPSNPGPGSWV
                     IGANLRLMQRQKAGVLHADFTACDGYKGGFERAAQVKCPALFLLGARDAMTPARAGRD
                     LARTVPNSTVVTLDSAGHNLMGEKPDEILDALVEFLRA"
     gene            791..1555
                     /locus_tag="VEM33_08035"
     CDS             791..1555
                     /locus_tag="VEM33_08035"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HYS51815.1"
                     /translation="MTARCTALALLPVVPVAALAQSLLPEWIGAGVRTRPAYDGSAAQ
                     RTELIPTVRYYGTPWFARTTQGIFEGGARMELARDFNVGAQLAYEGGRIASESDFLGR
                     NNVPDINPSASIGLHLEWDRNLGPMPVTLLARGRRFIDGDRGAQADLRFTTIVFGGGA
                     VTAAVFIQGTWANSKSNQSFYGITLAQGTGLPAYSAGSGLLYASGGLLWGVDLSREWI
                     VVGGLEARRLHGDAARSPLAERTSNQYASASLAYRF"
     gene            1887..>2440
                     /locus_tag="VEM33_08040"
     CDS             1887..>2440
                     /locus_tag="VEM33_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51816.1"
                     /translation="MTQPPSTPVAVTANNATQVSATALKPAVGGGALATAGGLETTAA
                     PQARVVTRALLAVARDSKTQLSASQGVVGAAAQTTCSFGGTKTADISADGTSGTFTFN
                     ACSDIAGETISGSISVTGVSATSDGSHFSGNFSEDITFTVTGFAPTRIVGSFSLDETC
                     SNFSTRDCTSRFTGTSLGAQQGAET"
BASE COUNT          415 a          823 c          804 g          398 t
ORIGIN      
        1 tccgcctacg cctacaccgg cgggaaggct ttcgatgcca agctcccggc ggtggtgttc
       61 attcacggcg gcgaacagga ccacagcgtt tgggccctgc agtcgcgcta tctcgcgcac
      121 cacggtcacg ccgtgctcgc ggtcgatctg ccggggcacg gcaggagcgg cggcgcgccg
      181 cttgcgagca tcgaggacat ggcgggctgg atcgtcgcct ccctcgacgc ggccaaggtc
      241 ggcaccgcgg ccctggtcgg ccacagcatg ggctcgctcg ccgcgcttga gtgcgctgca
      301 cggcatccgc agcgcgtcaa ccgcgtcgcc ctgctcggaa ccgcctttcc gatgcgcgtg
      361 tcgcccgatc tcctccaggc gacgaagcac cgggaatccg acgcccacgg catgatcaac
      421 gcctggtccc actccgccta cgcgcactac ccgagcaatc cggggccggg atcctgggtg
      481 atcggcgcga acctgcggct gatgcagcgg cagaaagcgg gggtgctgca cgccgacttc
      541 accgcctgcg acggatacaa gggcggattc gagcgcgctg cgcaggtgaa atgccccgcg
      601 ctgttcctgc tcggcgcgcg cgatgcaatg accccggccc gcgcgggacg ggacctcgct
      661 cggacggttc cgaattcgac cgtggtgacg ctggactccg ccggccacaa cctgatgggc
      721 gaaaagccgg acgaaattct cgatgcgctc gtcgaatttc tccgcgccta ggctgcgata
      781 cactctacgc atgactgcgc gatgcacggc gctcgctctt ctgccggtcg ttccggttgc
      841 ggccctcgct cagagcctct tgccggaatg gatcggggcc ggcgtgcgca cgcgccccgc
      901 gtacgacggc tcggccgcgc agcgaaccga gttgattccg acggtgagat attacggaac
      961 gccctggttc gcgcgcacca cccaggggat cttcgaaggc ggcgccagga tggagctcgc
     1021 gcgggacttc aatgtcgggg ctcaactcgc ctacgaaggc gggcgcatcg cgagcgaatc
     1081 ggatttcctc ggcaggaaca acgtccctga catcaacccg agcgcctcga tcggcctgca
     1141 cctcgagtgg gaccggaacc tcggcccgat gccagtcact ctgctcgcgc gcgggcgccg
     1201 cttcatcgac ggcgatcgcg gcgcgcaggc cgatctgcgc ttcactacga tcgtttttgg
     1261 gggcggcgcg gtcaccgccg ccgtattcat ccaggggacc tgggccaatt ccaaatcgaa
     1321 ccagtccttc tacggcatca cgctcgcgca gggcacgggc ctgcccgcct attcggccgg
     1381 aagcggcctg ctgtatgcaa gcggcggatt gctctggggc gtcgatctca gccgggaatg
     1441 gatcgtcgtc ggcggtctgg aggcgcgacg gctgcacggc gacgccgcgc gcagcccgct
     1501 tgcggagcgc acgtcgaacc aatacgcgag cgcaagcctg gcgtaccggt tctgaggcgg
     1561 acggcccagc ctcttggggt aggctcggta aaatcgacaa caattctcga ttaatcaaga
     1621 tcctcgggaa ttcgccctgg cgcccgggcc cgacggcatt gaatgcgcca ttcaacctat
     1681 attagacttg tcaacccaga gccgagatga agaagcggac gccttctcct cggcctaatt
     1741 atagggtccg cagggagagt tccatgcttg gcaaattcaa cgcggcaaaa gggtctactc
     1801 cgtatcggca ggtagcggcg ctgctcttcg cttttcttgg ggggtgtggc ggagggggcg
     1861 gcggcggtgg cggcgggccg accggattga cgcaaccccc cagcactccg gtcgccgtca
     1921 cagcgaacaa cgcgacgcaa gtcagcgcaa cggctctcaa acccgccgtc ggcggcgggg
     1981 cgctggctac agccggcggg ttggagacga ccgcggcacc gcaagcgcgc gtagtaacgc
     2041 gcgcgctcct tgcggtcgct cgcgactcga agacacagct ctccgcttcg cagggcgtcg
     2101 tgggcgcggc ggcccagacg acatgtagct ttggtggcac gaaaacggca gacatcagcg
     2161 cggacggcac ctccgggaca ttcactttta acgcctgctc cgacatcgcc ggcgagacga
     2221 tcagcggctc gatttcggtg acgggggtct ccgccacgtc ggacggaagc catttctccg
     2281 ggaacttcag cgaggacatc acgttcaccg tgaccggatt cgcgccgacg cggatcgtcg
     2341 gcagcttctc gctcgacgag acctgcagca atttctcgac tcgcgattgc acgagcagat
     2401 ttacggggac cagcctcggc gcccagcagg gcgcggaaac
//
LOCUS       DASPZD010000415         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_239329, whole genome shotgun sequence.
ACCESSION   DASPZD010000415 DASPZD010000000
VERSION     DASPZD010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239329"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VEM33_08045"
     CDS             complement(<1..400)
                     /locus_tag="VEM33_08045"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51817.1"
                     /translation="MNPTFSPAEQFTVAAQAFLRNFLASLGVAPDSEFARSLQSIGVD
                     STKLAALQARTFQMHAALWQSTLAREAGHDATPVAPSDRADRRFSSAEWQRVPWFDYL
                     RQSYLINSRFLSDWIEALEADPRAKERLRFI"
     gene            663..1415
                     /locus_tag="VEM33_08050"
     CDS             663..1415
                     /locus_tag="VEM33_08050"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYS51818.1"
                     /translation="MGHKPAVRLAQILANRSKTALEEAVLLTSEQLGFRYFMFCGHFS
                     RPRGATHEIRFDNFPVHWHRYCADRGRDLLPGPLRRLGLQEVTPLPWRKIATRYGSAF
                     AKAGEFGLATGVSCSVHGPRGQWSLTSFALARGGSAAERHILATLPNCQLVACAIHYA
                     AARLARRSVDRLGAPRELDSARNRLLSDRESQCLILSARGKTTSEIGQALQISERTVA
                     FHLSNLRRKLGATNSRHAVARAFSLKLIAAGS"
     gene            complement(1625..>2073)
                     /locus_tag="VEM33_08055"
     CDS             complement(1625..>2073)
                     /locus_tag="VEM33_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS51819.1"
                     /translation="PLGWHVVLHLDAEDIESHAELFHRIRVPFVIDHMGRIQAAYGLD
                     QRPFRMLLDLMTKNELAWVKVSGPERVSAGGKPFHDAMPFAAALVESAPERVLWGSDF
                     PHPNVKTMPNDGELVDFFAKVVPDEKLRKRILVDNPARLYWTAARN"
BASE COUNT          392 a          655 c          652 g          374 t
ORIGIN      
        1 cgataaagcg caggcgctct tttgcgcgcg gatcggcttc gagcgcttcg atccaatccg
       61 atagaaagcg cgaattgatg aggtagctct ggcgcaggta gtcgaaccag ggaacccgct
      121 gccattccgc ggaggaaaag cgccggtcgg cgcgatcgga cggcgcgacg ggcgtggcgt
      181 cgtggccggc ctcacgggca agcgtcgatt gccacagcgc cgcgtgcatc tggaatgtgc
      241 gcgcttgaag ggccgcgagc ttggtggagt caacgcctat cgactgcagc gagcgggcga
      301 attcggaatc gggcgcgaca cccagcgacg cgaggaaatt cctcagaaac gcctgggcgg
      361 cgaccgtgaa ttgctcggcc ggagagaacg ttgggttcac cacgcagtct ttcgctgaaa
      421 accttggaat gtccgagaag gcgacgggat attgcgccgc acaatggacg aagtcaaggc
      481 aggaccactg atacttcagc taccgagcta acggcctatc cgaactctga aatcagcgct
      541 cgcagcgcgt gcgcatgtac ggtgcgggtg ccggaaaggg aagctgcttg cgtagcgccg
      601 gcgaaagggt agagtatcag ccagaataag gggactgacc ccgcagggga gagggacaaa
      661 gcatggggca caagccggcc gtgcgtcttg cgcagattct tgccaaccgc tcgaagacgg
      721 ccctcgagga agcggtgctc ctcaccagcg agcaactcgg cttccggtac ttcatgttct
      781 gcggccactt ctcgcggccg cgcggcgcca cgcacgaaat ccggttcgat aattttcccg
      841 ttcactggca ccgatactgc gccgatcgtg gccgggatct cctgcccggt ccgctgcgcc
      901 gtctcggcct gcaggaggtc acccctttgc cctggagaaa aatcgcgacg cggtatggca
      961 gtgccttcgc caaggcgggc gaattcggtc tggcaacggg ggtttcctgc tccgtccacg
     1021 gacctcgggg acaatggagc ctcacaagct tcgcgctcgc tcgcggcggc tccgcagcgg
     1081 aacgccatat cctcgcgacg ctcccgaatt gccaactcgt cgcgtgcgcc attcactacg
     1141 cggccgcgcg cctcgcacgg cggagtgtcg accggcttgg ggcgccacga gagctcgaca
     1201 gtgctcggaa ccggcttctc agcgaccgcg agagccaatg tttgatcctc tcggcgcgcg
     1261 gcaagacgac ttctgaaatc ggccaggcgt tgcagatttc cgagcgcacg gtcgcgttcc
     1321 atctctcaaa cctgaggcgc aagctcggcg ccaccaactc ccgtcacgct gtcgcgaggg
     1381 ccttctcgct caagctgatc gctgcgggtt catagctgaa cgtctgcgcg atcgcgcaga
     1441 cgatccgaat cgcctgcctg aaagtgatct tcaacgacgg gttgaccacg gtaaggcagg
     1501 tcgaactcga ggattgaacg ttccgcgcgc ggccgaaaaa cctcggccgc gcatcagctt
     1561 atccgcccgt ctaggaagcg gcggtgaaac gggggaacct ccctcccccg ttttttttcg
     1621 cacgtcagtt ccgcgcggcc gtccaataga ggcgcgccgg gttgtccacc aggatccgtt
     1681 ttctgagctt ctcgtctggt acgactttcg cgaaaaagtc gaccagctcg ccgtcgttcg
     1741 gcatggtctt cacgttggga tggggaaagt cgcttcccca cagcacccgc tccggcgccg
     1801 actcgaccag cgccgcggcg aacggcatcg cgtcgtgaaa tggctttccg cctgcggaca
     1861 ctcgctccgg tccgctcact ttcacccagg ctagctcgtt tttcgtcatc agatcgagca
     1921 gcatgcgaaa aggccgctga tcgagcccgt acgcagcctg tatgcgtccc atgtggtcga
     1981 tcacgaaagg tacgcgaatt cggtggaaca attcggcatg cgactcgatg tcctccgcgt
     2041 cgaggtgcag gaccacgtgc cagccgaggg gct
//
LOCUS       DASPZD010000416         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_843144, whole genome shotgun sequence.
ACCESSION   DASPZD010000416 DASPZD010000000
VERSION     DASPZD010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843144"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VEM33_08060"
     CDS             complement(<1..430)
                     /locus_tag="VEM33_08060"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYS51820.1"
                     /translation="MKAFLQSPLAAIAAALVAGVLTVAAFAPLGVFPLSFLTLAGLLL
                     IWLRAATPQVAFWAGFAFGAGLFGAGASWVYVSLHDFGMMPAPLAAIGTIAYCAILAL
                     YPGAAGWCLARLARAPLGSVLIAFPAAWTLFEWLRGWVFTG"
     gene            complement(427..1257)
                     /locus_tag="VEM33_08065"
     CDS             complement(427..1257)
                     /locus_tag="VEM33_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HYS51821.1"
                     /translation="MDESNKPSLLERLSALLMREPEDREQLLKLLHSAFERNLLDGDA
                     LSMIEGALQVSEMQVRDIMVPRAQMDMIDVNETPDKFIPHVIATAHSRFPVIDKNRDD
                     VLGVLLAKDLLRSYAGEEEFKVRDMLRPAVFVPESKRLNVLLREFRANRNHMAIVVDE
                     YGGVAGLVTIEDVLEQIVGDIEDEYDFDDSEDNIVREQGGHYRVKAQTEIADFNRAFG
                     AKFSDTDFDTVGGLVVNRFGRLPKRGEAITIDGLRIQVLRADSRRLHVLQVHRLPGKA
                     "
     gene            complement(1310..1843)
                     /gene="ybeY"
                     /locus_tag="VEM33_08070"
     CDS             complement(1310..1843)
                     /gene="ybeY"
                     /locus_tag="VEM33_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HYS51822.1"
                     /translation="MPTSVTGTSNVMRAGGSSGTARPARSRRSPKPRLTLHLQVVSRA
                     APVPASAQIRRWARAALKGSAEITVRIVGAAEARTLNRRYRGRDYAANVLSFPYALRH
                     GLVQGDIVLCGPVIAREALTQGKAVEAHLAHLTVHALLHLQGYDHLRRRDAARMEARE
                     KKLLAKLGYPDPYCAPG"
     gene            complement(1815..2765)
                     /locus_tag="VEM33_08075"
     CDS             complement(1815..2765)
                     /locus_tag="VEM33_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYS51823.1"
                     /translation="MRRRPLKTGPLELVLTPVDNQRLANLCGTLDENLRQIETALDVT
                     IARRGERFTVRGAHAAQAADVLQKFYDDAKRGLTLDDIQLGLIEVRSRGRAKEETPLL
                     TRRADLHGRTPRQRQYIQNILEHDITFAIGPAGTGKTYLAVACAVDALERDAVKRIVL
                     VRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLLGFDKAGKLFDRGAIELAPLAY
                     MRGRTLNHSFVILDEAQNTTPEQMKMFLTRIGFGSKAVVTGDITQIDLARGQKSGMVA
                     VQKILAQVRGIAFTAFKSEDVVRHPLVQRIVDAYERHGDE"
     gene            complement(2755..>2900)
                     /locus_tag="VEM33_08080"
     CDS             complement(2755..>2900)
                     /locus_tag="VEM33_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HYS51824.1"
                     /translation="AGELSGRAGDNRVVNFRGPRPLVGQFVEVAITAAMAHTLRGEVR
                     VAA"
BASE COUNT          493 a          948 c          979 g          480 t
ORIGIN      
        1 cccccgtgaa gacccagccg cgcagccact cgaacagcgt ccacgccgca gggaacgcga
       61 tcaagacgga gccaagcgga gcgcgcgcta gccgcgcgag gcaccagcca gcggcgcccg
      121 gatacagcgc gaggatcgcg cagtaggcga tcgtgccgat cgcagcgagc ggtgccggca
      181 tcatcccgaa atcgtgcagg ctgacgtaga cccaggaagc accggcgccg aagagtcctg
      241 cgccgaaggc gaagcccgcc cagaacgcga cctgtggcgt tgccgcgcgc agccagatga
      301 gcagcaggcc tgcgagagtc aagaacgaca gcgggaatac cccgagtgga gcgaatgcgg
      361 cgacggtcaa gacgcccgcg accagagccg cggcaatcgc tgcgagcggc gattggagaa
      421 aagccttcaa gccttgcctg gcagccggtg cacctgaagg acgtgcagcc tgcggctgtc
      481 ggcgcgcagc acctggatgc gcaggccgtc gatggtgatc gcctcgccgc gcttgggaag
      541 gcgcccgaag cggttgacca cgagcccgcc gacggtgtcg aaatcggtgt cggagaactt
      601 cgccccgaag gcccgattga aatcggctat ttccgtctgc gccttgacgc ggtagtgtcc
      661 cccttgctcg cgcacgatgt tgtcttcgga atcgtcgaag tcgtattcgt cctcgatgtc
      721 gccgacgatc tgctccagca cgtcctcgat ggtcaccagc ccggccactc cgccgtactc
      781 gtcgaccaca atcgccatgt ggttgcggtt cgcgcggaat tcgcgcagca gcacgttcag
      841 ccgcttggac tcgggcacga agacggcggg tctgagcatg tcgcgcacct tgaactcttc
      901 ctcccccgcg taggagcgca gcaggtcctt ggcgagcagc acgccgagga cgtcgtcgcg
      961 attcttgtcg atgaccggga atcgcgagtg cgccgtggcg atcacgtgcg gaatgaactt
     1021 gtcgggggtc tcgttgacgt cgatcatgtc catttgtgcg cgcggcacca tgatgtcgcg
     1081 cacctgcatc tcggagacct gcagcgcgcc ctcgatcatc gacagggcgt cgccgtctag
     1141 gagatttctc tcgaaggcgg agtgcagcag cttgagcagc tgctcacggt cctccggctc
     1201 gcgcatgagc agcgcggaaa gccgttcgag cagcgaaggt ttgttcgatt cgtccatgtt
     1261 tgccgcgggg cctggcgcca cgagctcgct tgcccaagcc tgggagtcct cacccgggag
     1321 cgcagtaggg atcaggataa ccgagtttgg caagaagctt cttttcccgg gcttccatgc
     1381 gcgcagcgtc acgccgccta aggtgatcgt agccctgcaa gtgcagaagc gcgtggaccg
     1441 tcaggtgcgc aagatgcgct tccaccgcct tgccttgcgt gagcgcttct cgagcgatca
     1501 cggggccgca aaggacgatg tcgccctgca ccaagccgtg ccgcagcgca tagggaaaac
     1561 tcagcacgtt ggcggcatag tcgcggcccc gatagcgacg attgagggtg cgagcttcgg
     1621 cggctccgac tatcctcacg gtgatctcgg ccgagccttt gagcgccgcg cgcgcccatc
     1681 gacgaatctg cgccgaagca ggcacaggcg cggcgcgcga aacgacctga agatggagcg
     1741 tgaggcgcgg ctttggggag cgcctcgatc gtgcgggccg ggctgtgccc gacgagccgc
     1801 cggccctcat gacgttactc gtccccgtga cgctcgtagg catccacgat gcgctgcacc
     1861 agaggatggc gaacgacgtc ctcgctcttg aacgcggtga acgcgatacc ccgcacctga
     1921 gcgagtattt tctggaccgc caccatgccg ctcttctgcc cacgcgccag gtcgatctgc
     1981 gtgatgtcgc cggtcaccac cgccttcgag ccgaagccga tgcgcgtgag gaacattttc
     2041 atctgctcgg gggtggtgtt ctgggcctcg tcgagaatga cgaacgagtg gttcagcgtg
     2101 cggccgcgca tgtaggcgag cggagcgagt tcgattgcac cgcggtcgaa gagcttcccg
     2161 gccttgtcga aacctagaag gtcgtagagc gcgtcgtaga gcggccgcag gtacggatcg
     2221 accttctgag caaggtcgcc gggcaggaac cccagacgct cgcctgcctc gactgcgggc
     2281 cgcaccagca cgatgcgctt gacggcgtcg cgctccagcg cgtccaccgc gcacgccacc
     2341 gcgaggtagg tcttgccggt gcccgcggga ccgatcgcga acgtgatgtc gtgctcgagg
     2401 atgttctgga tgtactgccg ctgccggggc gtgcggccgt gcaggtccgc ccgccgcgtg
     2461 agcagcggcg tctcctcctt cgcccggccg cgggagcgga cctcgatcag gccgagctgg
     2521 atgtcgtcca gggtgaggcc gcgcttggcg tcgtcgtaga atttctgcag cacgtctgcg
     2581 gcctgggcgg cgtgcgctcc gcggaccgtg aatcgctcgc cgcggcgcgc gatcgtgacg
     2641 tccagcgcgg tctcgatttg cctcaggttc tcgtccagcg tcccgcagag attggcgaga
     2701 cgctggttgt cgaccggagt gaggacgagt tccagggggc cggtcttcaa aggcctacgc
     2761 cgcaacgcgg acctcgccgc gcagcgtatg cgccatcgcc gcggtgatgg ccacctcgac
     2821 gaactggccc acgagcggcc ggggaccgcg aaagttgacg acgcggttgt ctcccgcgcg
     2881 gcccgagagc tccccggcat
//
LOCUS       DASPZD010000417         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_477697, whole genome shotgun sequence.
ACCESSION   DASPZD010000417 DASPZD010000000
VERSION     DASPZD010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477697"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="VEM33_08085"
     CDS             complement(<1..1411)
                     /locus_tag="VEM33_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HYS51825.1"
                     /translation="MSIVCGTAMAQETSRITSVILYPGSATVERSARVVPGMTRLEIS
                     GLPANFDPQTVRVESDAGVRIGEVSTKDQSRTAASNARESAIEERIQALKDRQAVLEV
                     DARSAQMTADFISRLGAPAGEKPAPLPSARSLAETIEVVRRGGSDAFGRIQKVQVQKR
                     EIDKQIRALERDLARLKSGANDVRTIAVGVSAERPGEVRVSYQVNGAGWRPAYRAGLD
                     SGTSKVLLERLGAISQTTGEDWTNVRLKLSTGQPRLSPQGTEPRPWKLSLYSPHADRL
                     SEISSGIVASRAPAALMRAKADELRQETPVELQTTFATEFEVPGAVSLPSDGRKLTVS
                     LAKLSLPAKMRLRVVPRLDAAAVVTAEAERPEGVWLAGDIQLYRDGNYVGATSWNPQS
                     SAALVLPFGRDSLVRVKVDRAKDRNGSGGIIAQRNQREVSDLYTLTSHHKTPVELLVL
                     ESSPVSTNDQIKVEAKFDPK"
     gene            complement(1533..>2327)
                     /locus_tag="VEM33_08090"
     CDS             complement(1533..>2327)
                     /locus_tag="VEM33_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51826.1"
                     /translation="KQQVIVDNRPGAGGTLAAGMVARAAPDGATLLSVSSAHAIAPIL
                     YSNLTYDTFRDLSGITLSAASKYVLVASPSLGIKSVKELIAAAKAKPGTFNFSSAGMG
                     SGTHFAGEIFKAMAAIDVVHVPFKGIPEALTETLTGRVQFFMAPIANAVAHVREGKLT
                     GLGVSSAERDPLLPEVPTVAEAGVPGYESILWFGLLTSSAVPKPIVTKLNREIVRILS
                     DPDARQRWTPIGLQPQPTTPEGFDKLIRDDVATFAKIARGANIKAE"
BASE COUNT          364 a          778 c          757 g          428 t
ORIGIN      
        1 gtttcggatc gaatttcgcc tcgaccttga tctggtcgtt ggtggagacg ggcgaggatt
       61 ccagcaccag cagttcgacc ggtgtcttgt gatggctcgt gagtgtgtag agatcgctca
      121 cctcgcgctg gttgcgctga gcgatgattc cacccgagcc gttcctgtcc ttcgcgcgat
      181 cgaccttcac gcgcaccagg ctgtcgcggc cgaacggcag cacgagcgcc gccgaggact
      241 gcggattcca gctggtcgcg ccgacgtagt tgccgtcgcg gtagagctgg atgtctccgg
      301 cgagccacac gccttcgggg cgctcggcct ccgcggtgac gaccgccgca gcatccagcc
      361 tcggcaccac gcgcaggcgc attttcgcgg ggagcgaaag cttcgcgagc gacacggtca
      421 gcttgcggcc gtccgagggc aggctcaccg cgccgggaac ttcgaactcg gtggcgaagg
      481 tggtctgcaa ttcgacgggc gtttcctggc gcagctcgtc ggctttcgcg cgcatcagcg
      541 cggcgggcgc tctggacgca acgatgccag atgaaatctc cgacaggcga tccgcgtgcg
      601 ggctgtaaag cgacaacttc cacggacgcg gctcggtccc ttgaggcgag agccgcggct
      661 ggccggtgga gagtttcagc ctcacgttcg tccagtcctc tcccgttgtc tgcgagatgg
      721 cgcccagacg ctcgagcagg actttcgacg tccccgagtc caggccggcg cggtacgccg
      781 gccgccatcc ggcgccgttc acctggtagg acactctcac ctcgcccggc cgctcggcgg
      841 agacgccgac tgcgatcgtg cgcacgtcgt tggcgccgct cttcagccgc gcgagatcgc
      901 gctcgagcgc gcggatctgc ttgtcgatct cgcgcttctg cacctgcacc ttctggatcc
      961 tgccgaaggc gtcgctgcct ccgcgccgca ccacctcgat cgtttcggcg agcgatctcg
     1021 cgctcggaag aggcgcgggc ttctcccctg ccggtgcgcc caggcgcgaa atgaaatcgg
     1081 ccgtcatctg ggcggacctg gcatcgacct cgagaaccgc ctgccggtcc ttgagcgcct
     1141 ggattctctc ctcgatcgcg gactcgcgcg cgttcgaggc tgcggtgcgg ctctggtcct
     1201 tcgtggagac ctcaccgata cgcacgcctg cgtccgattc cacgcgcacc gtctgcggat
     1261 cgaaattcgc cggcagcccg gagatttcca ggcgcgtcat gcccggcacg acccgcgcgc
     1321 tgcgctccac cgtcgcgctg ccggggtaga gaatcacgga ggtgattcgg gatgtttcct
     1381 gcgccattgc cgtgccgcag acaattgaca ttgcgagcag cccagtccca atactcgtcc
     1441 ttcgatgaag cagcattgtt tacccctctc atccggcgtc acgccgtttc aatgtcggta
     1501 aacgcgccgg cggcactgac ggggttaaaa cgctactcgg cttttatatt cgcccctctc
     1561 gcaatcttcg caaacgtcgc gacatcgtcc cgaattagct tgtcgaaccc ttccggcgtg
     1621 gtcggttgcg gctgcaggcc gatcggcgtc cagcgctgtc tcgcgtcggg gtcggagagg
     1681 atgcgcacga tttcgcggtt gagcttcgtc acgatcggtt tcgggacagc ggatgaggta
     1741 aggaggccga accacaggat cgattcgtag cccggaacgc cggcctccgc gacggtcggc
     1801 acttcgggca gcagcgggtc gcgctccgcc gatgagacgc ccaggcccgt gagcttgcct
     1861 tccctcacgt gagccacagc attcgcaatg ggcgccataa agaactgcac gcgcccggtc
     1921 agcgtctcgg tgagcgcctc ggggatgccc ttgaagggaa cgtgcacgac gtcgatcgcc
     1981 gccatcgcct tgaaaatctc tccggcgaag tgcgtgccgc tgcccatccc ggcggaggag
     2041 aagttgaagg tacccggctt cgctttcgcc gccgcgatca gctccttcac cgacttgatg
     2101 ccgagcgaag gcgacgcgac gagcacgtac ttcgaggctg cggagagcgt gatgcccgaa
     2161 agatccctga acgtgtcgta ggtgagattc gaatagagga tgggcgcgat ggcgtgcgcg
     2221 gaggagaccg agagcagcgt cgcgccgtcg ggcgcggcgc gcgccaccat tcccgcagcg
     2281 agcgtgccgc ccgcgcccgg gcgattgtcg acgatcacct gctgctt
//
LOCUS       DASPZD010000418         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1391945, whole genome shotgun sequence.
ACCESSION   DASPZD010000418 DASPZD010000000
VERSION     DASPZD010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391945"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VEM33_08095"
     CDS             complement(<1..590)
                     /locus_tag="VEM33_08095"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzing the hydrolysis of
                     4-imidazolone-5-propionate to N-formimidoyl-L-glutamate,
                     the third step in the histidine degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HYS51827.1"
                     /translation="MRRLSNIGRLFTGTPTGVIEGAAVICDGEEIAWCGKSGDEPRDL
                     MESVTDENDCQGGLVTAGLIDAHTHPVYAGDRSAEVAMRSAGATYAEIAKVGGGIVAT
                     VKATREEAISALEQATARRLWNWLEGGATTVEAKTGYHLQREGELEATRLLARLAQRA
                     DLPRIEITFLAAHALPPDRWARMSSYAKEVGEWCEDA"
     gene            complement(587..1816)
                     /locus_tag="VEM33_08100"
     CDS             complement(587..1816)
                     /locus_tag="VEM33_08100"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HYS51828.1"
                     /translation="MNGSGAWHAEQAWLGRRAENVLIQVEDGRIKAIKGDTLAPPDAV
                     VLKGWTVPGLANVHSHAFQRLLRGEIESGAGDFWEWRDRMYRFTEWEPADYFNHARLV
                     FREMLEAGITAVGEFHYLHGHGNELGEALIDAAREEGIRITLIDACYLRAGLDGRPLE
                     AEQLSFSDGDADSWARRVDELSDADGVRIGAAIHSVRAVDAESMRVVATYARERKAPL
                     HIHLAEQPAEVEECLEVERCTPAELLEREGILGPDLTAIHAIHVNEGDVAILGQNQVS
                     ICACPTTERDLGDRVGPLRALASAGCPLNLGSDSNAVIDILEDARALELDQRRALGRR
                     VLHQPADLLRAATADGMRSLGWDAGELKVGMLADFITLDQPRGQWRELNPAFLMYGFS
                     GHDVTNVVVGGNTVVSR"
     gene            complement(1813..>2116)
                     /locus_tag="VEM33_08105"
     CDS             complement(1813..>2116)
                     /locus_tag="VEM33_08105"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353723.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYS51829.1"
                     /translation="ESMRDGSDAIADWPILNALLNTSSGASWVSVHHGGGVGIGRSIH
                     AGMVVVADGSEVAAKKLERVLTNDPGTGVMRHADAGYERAVEVARDRGVRLPMLKD"
BASE COUNT          327 a          754 c          671 g          364 t
ORIGIN      
        1 gcgtcttcgc accactcacc cacctctttc gcgtacgagc tcatgcgcgc ccagcgatca
       61 ggcggcagcg cgtgcgccgc gagaaacgtg atttcaatgc gcgggaggtc cgcgcgctga
      121 gcaagacgcg cgagaagccg ggtggcctcc agctcgcctt cgcgctggag gtgatagcca
      181 gtcttcgcct cgaccgtggt ggcgccacct tcgagccagt tccacagcct gcgcgcggtc
      241 gcttgctcga gggcgctgat cgcctcctcg cgcgtcgcct tcacagtcgc gacgattcct
      301 ccgcccacct tcgctatctc ggcgtaggtc gcgcccgcgg aacgcattgc gacctccgcc
      361 gaccgatcgc cggcgtagac ggggtgagtg tgtgcgtcga tgagaccggc cgtcaccagc
      421 ccgccctggc agtcgttctc gtcggtgacc gattccatga ggtcgcgcgg ctcatcgccg
      481 gactttccgc accaggcaat ctcctcaccg tcgcagatga ccgcggcgcc ctcgatcacg
      541 ccggtgggcg tgccggtgaa caggcggccg atgttgctga ggcgcctcat cgcgaaacga
      601 ccgtgttgcc gccgaccact acgttggtga cgtcgtgacc cgagaacccg tacatgagaa
      661 aggcggggtt gagctctcgc cactgaccac gtggttggtc gagtgtgatg aagtccgcga
      721 gcattccgac cttcaactcg ccggcgtccc agcccagcga ccgcatgcca tcagccgtgg
      781 ccgcgcgaag caagtccgcg ggctgatgaa gcacccggcg gccaagggcg cggcgctggt
      841 cgagctccaa cgcgcgcgcg tcttcgagga tgtcgatcac cgcgttggaa tcgctgccca
      901 ggttcagggg gcagccggcc gacgccagag cccgtagcgg cccgaccctg tctcccaggt
      961 cacgctctgt ggtcgggcat gcgcagatcg aaacctggtt ctggccaaga atggcgacgt
     1021 cgccctcgtt gacgtggatg gcgtggatgg cagtgagatc tgggcccagg atgccttctc
     1081 gttcaaggag ctcggcgggc gtgcatctct cgacttccag gcactcttcg acctccgcgg
     1141 gctgctcggc gaggtggatg tgcagcggcg ccttgcgctc ccgcgcgtaa gtggccacga
     1201 cgcgcatcga ctccgcgtcc accgcgcgga cgctgtggat ggccgccccg atacgcacgc
     1261 cgtccgcatc gctcagctcg tcgacccgtc tcgcccagct gtccgcgtcg ccgtctgaaa
     1321 acgataactg ctccgcttcc agcggccggc catcgagacc tgcgcgcagg tagcaggcgt
     1381 cgatcagagt gatccggatg ccttcctccc gagccgcgtc gatgagcgcc tcgcccagct
     1441 cgttgccgtg gccgtgcagg tagtggaact cgcccaccgc ggtgatgccc gcctcgagca
     1501 tctcgcggaa aaccagacgc gcgtggttga agtagtcggc gggctcccac tcggtgaagc
     1561 gatacatccg atcgcgccac tcccagaagt cgcccgcccc ggactcgatc tcgccccgaa
     1621 gaaggcgttg aaacgcgtga ctgtgcacgt tcgccagccc tggaaccgtc catcccttga
     1681 gcacgaccgc gtccggcggc gccaacgtgt cgcccttgat tgccttgatg cgtccgtctt
     1741 cgacctggat caagacgttc tcggcgcgac ggccgagcca cgcctgctcg gcgtgccacg
     1801 cgccagatcc gttcaatcct tgagcatggg gaggcgaacg ccgcggtcgc gtgccacctc
     1861 gaccgcgcgt tcatagcccg catcggcgtg ccgcatgaca cccgtgcccg ggtcgttcgt
     1921 cagcacgcgc tcgagcttct tcgcggcgac ctcgcttcca tccgcgacca cgaccatgcc
     1981 ggcatggatc gagcgaccta tgccgacccc accgccgtgg tgcacgctga cccacgacgc
     2041 gccgctcgat gtgttgagaa gtgcgttcag gatgggccag tccgcgatgg catccgagcc
     2101 gtcacgcatg gactcc
//
LOCUS       DASPZD010000419         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_650750, whole genome shotgun sequence.
ACCESSION   DASPZD010000419 DASPZD010000000
VERSION     DASPZD010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650750"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VEM33_08110"
     CDS             <1..661
                     /locus_tag="VEM33_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYS51830.1"
                     /translation="AKLLRVLENGEFQRVGETQQRFSRSRVIAATNRDLRQEVRKGNF
                     RADLYHRLSVFTIEVPSLREMEDDCTVLLEHFRKFYAEQAKLAPFALDDSAQKAWRDY
                     HFPGNVRELRNVVIRLTTKYAGQKLSIAELEPEFDLEAQADGGPGLPSDPGTLIEQAQ
                     RHLQRERSFNLDQMLKAWEQGYIEAAMKLTRGNVSQAAKMLGINRTTLYSRMESAQKQ
                     Q"
     gene            691..2106
                     /locus_tag="VEM33_08115"
     CDS             691..2106
                     /locus_tag="VEM33_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYS51831.1"
                     /translation="MYLDHFGLKEPPFRITPDTDFFFQGANRGATLEALLYAITHDEG
                     IVKVSGEVGSGKTMLCRVLMERLPATVETIYLANPSLSRDEILFAIGDELKLQLEKGP
                     RILRALQEHLIRLFGEGRRVVVLIDEAHAMPDETLEEIRLLSNLESNRHKLLQIVLFG
                     QPELEEHLDTAGMRQLRERITQSFHLEPLVRSDIENYIDFRMRAAGYRGPKVFNPRAI
                     RLIASASQGLTRRVNILSDKALLAAYAAGTHAVTQAEAKRAVRDTEFYRTRTSMQRIW
                     IGATALAAGLALGWGTHTLLSPARPSIVSVPPPAATSTAAEAPAGPAKAAMPLVSGLS
                     PSASTDASALKPSAEISTLAQSPRPERGELARRRFAATQEWLKSAPGDRYAIQLAIVN
                     SKELGQLEDFLLRTSKILPVDELFVYSVKIDGQQHYRVAYGSYASRDQALVAIKGLPP
                     VISAYHPYYRSVERMRSQNRQ"
     gene            2367..>2994
                     /locus_tag="VEM33_08120"
     CDS             2367..>2994
                     /locus_tag="VEM33_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYS51832.1"
                     /translation="MPAPIQISTILPKPKPTQRPETYSVVVNGVRAQELLFALARDAK
                     LNIDVHPGITGAVTLNAIEQTLPQLLSRISRQVDMRWELDGPNLIVMPDSPYLHTYRI
                     DYVNMERTSTGTVGVSSQIGTGGGTAGGGGAGAAGGTGGGNTSSTTVLNTSSNKFWAT
                     LEKNIKDILHETDKVLPTGAPQPTLVQALTGAPGSAPPAPTAPNVSFRE"
BASE COUNT          595 a          958 c          931 g          510 t
ORIGIN      
        1 agccaagctt ctgcgcgtgc tcgagaacgg cgagttccag cgagtcggcg agacgcagca
       61 gcgtttctcc cgttcccggg tcatcgccgc caccaatcgc gacctgcgcc aggaagtcag
      121 aaaggggaac ttccgcgccg acctttatca ccggctgtcg gtgttcacga tcgaggtgcc
      181 gtcgctgcgc gagatggagg acgactgcac ggtgctgctc gaacacttcc gcaagttcta
      241 tgcagagcaa gcgaagctcg cgccgttcgc actggacgat tcagcgcaaa aggcctggcg
      301 cgactatcac tttcccggca acgtgcgcga gctgcgcaac gtcgtcatcc ggctgaccac
      361 caagtacgcg gggcagaagc tgtctattgc ggaactcgaa cccgaattcg atttggaagc
      421 gcaggccgac gggggaccgg gattgccgtc ggatccggga acgctcatcg agcaggctca
      481 gcgacatctg cagcgcgagc gcagcttcaa tctcgaccag atgctcaagg cgtgggaaca
      541 gggctacatc gaggccgcga tgaagctcac gcgaggcaac gtcagtcagg cggccaagat
      601 gctcggaatc aaccgcacga cgctgtactc acgcatggaa tcggcgcaaa agcagcaata
      661 ggcccagggg cccgctccgg gagggcatcg atgtacctag accacttcgg gttgaaagag
      721 ccgccctttc gcatcactcc cgacaccgat ttcttctttc agggcgccaa ccggggggcg
      781 accctcgaag ccctgctcta cgccattacc catgacgagg gcatcgtcaa ggtctcaggc
      841 gaggtcggca gcggcaagac gatgttgtgc cgcgtactga tggagcggct gcctgccacg
      901 gtcgaaacca tttatctcgc caacccgtcg ctgtcgcgag acgaaatcct attcgcgatc
      961 ggcgacgagc tgaagctcca gctggaaaaa ggaccgcgca ttcttcgggc actgcaggag
     1021 catctcatcc ggctcttcgg cgagggccgc cgggtggtgg tactgatcga cgaagcccac
     1081 gcgatgcccg acgaaacgct cgaggagatc cggctgttgt cgaatctcga atcgaaccgc
     1141 cacaagctgc tgcagatcgt gctcttcggg cagcccgagc tggaggagca cctggacacc
     1201 gccggcatgc gccagctgag ggagcgcatc acgcagagct ttcacctgga gcccctcgtg
     1261 cgcagcgaca tcgagaacta catcgacttt cgcatgcgcg ctgcgggcta ccgtggcccc
     1321 aaggtcttca atccgagagc gatcaggctc atcgccagcg cctcgcaggg tctcacgcgc
     1381 cgcgtcaaca tcctttcgga caaggccttg ctcgcggcgt acgcggcggg cacccatgcc
     1441 gtcactcagg ctgaagccaa aagggccgtg cgcgacaccg agttttatcg aacgcggacc
     1501 agcatgcaaa ggatctggat cggtgcgact gcgctcgccg ccggccttgc gctcggatgg
     1561 ggaacacaca cgctcctttc gcccgctcgg ccaagcattg tgtccgtccc cccgcccgcc
     1621 gcgacgagca ccgcggcgga ggcgcccgcc gggcccgcga aagcggcgat gccccttgtt
     1681 tcgggcttgt ccccgtcggc gagcacggat gcgtcggcgc tcaaaccaag cgcggaaatc
     1741 tcgacgctcg cgcagtctcc gcgtcccgag cggggtgaac tcgcccgaag gcgctttgcc
     1801 gcaacgcaag agtggctgaa gagcgcgcct ggggaccgct acgcaatcca gttggctatc
     1861 gtcaattcga aagaactagg gcagctggaa gacttcttgc ttcgaacatc caagatcctg
     1921 ccggtcgacg agctattcgt gtacagcgtc aagatcgacg gccagcagca ttaccgtgtt
     1981 gcctacggat cctacgcgag ccgggatcag gcgctggtcg ccataaaagg actgccgccg
     2041 gtgatttccg cgtaccatcc gtattaccgg agcgtggagc gaatgcgaag ccagaatcgc
     2101 cagtaataat cgcggccggt tcgggcaaca gcggaagctc tgatgtgcgt ggcaagagtt
     2161 ctttggtttg ttaaatcagt ttgttacatg gtactgttat actggtccgg gaaattctac
     2221 ccggcaggtt ggagcgggga tcggaggcca tcttgcgacg catctggctg gcgggtgttt
     2281 tgttcgcgct cggggcgtgc gctcacgcac cgattcagcc atcggacacg caccttcggg
     2341 ccgaggggcc ggcgccttcc ggaaccgtgc ccgctcccat acagatctcg acgatcctgc
     2401 ccaaacccaa gccgacccag cgacccgaga cttacagcgt ggtggtcaac ggggtcaggg
     2461 cgcaggagct gctgttcgcc ctcgcgcgcg atgccaagct gaacatagac gttcacccgg
     2521 gaatcaccgg ggcggtcacg ctcaacgcga tcgagcagac tctgccacag ctcctgtcgc
     2581 gtatttccag gcaggtcgat atgcgctggg aactggacgg ccctaatctg atcgtgatgc
     2641 cggattcacc gtatctgcat acgtaccgga tcgattacgt caacatggag cggacctcca
     2701 cggggacggt cggcgtctct tcgcagatcg gtaccggcgg cgggaccgca ggcgggggag
     2761 gggccggagc cgcgggcggg acgggtggcg gcaacacctc cagcaccacg gtactcaata
     2821 cttccagcaa caagttctgg gccacgctgg aaaagaacat caaagacatc ctgcacgaga
     2881 ccgacaaggt gttgcccacc ggcgctcccc agccgaccct tgttcaggcg ttgacgggcg
     2941 cgcccggatc ggctcccccg gcgcccaccg cgcccaatgt cagcttccgg gaag
//
LOCUS       DASPZD010000420         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1039167, whole genome shotgun sequence.
ACCESSION   DASPZD010000420 DASPZD010000000
VERSION     DASPZD010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039167"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VEM33_08125"
     CDS             complement(<1..667)
                     /locus_tag="VEM33_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYS51833.1"
                     /translation="MNELQRYLVEEAVEDYEEGRLTRREALKAIAGITGAALAAQMLE
                     ARAQVAPAGKAPASSAPLVAPNDPSIVAGIAEFPGEGVRLDGYLARPAGTERHAIVLV
                     CHENRGLTRHIEDVARRVAKAGYVGLAVDLLSREGGTAKHEFDAIPGILGKAAPARHV
                     QDFKSGLAYARSLPWARADRVGMTGFCFGGAVTWRVAAGVPDLRAAVSFYGVPVQEPD
                     VPGI"
     gene            complement(772..1359)
                     /locus_tag="VEM33_08130"
     CDS             complement(772..1359)
                     /locus_tag="VEM33_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYS51834.1"
                     /translation="MPDLEDQLAISALMQDWALARDTGDWGRLRATAHPGAAMTTTWF
                     DGRFEDFVESCEASFGKGSRSQHFLGGTSAEIKGARAIVQTRMSINVRSRLDGVEVDA
                     VCSGRFFDQVEKRDGVWRISRRSVIYEKDRIDPVDPSASISLDAGLLARFPEGYRHLA
                     YLQTKNGARVNPNLPTARGEALEKLIAEARAWLGS"
     gene            complement(1364..2302)
                     /locus_tag="VEM33_08135"
     CDS             complement(1364..2302)
                     /locus_tag="VEM33_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51835.1"
                     /translation="MAAFSAFSAASAQDYPSRPVRIVVPSAPGGGFDLVGRVLAQKLG
                     EQTGQAFVVENRPGAGTLVGTQLVARSAPDGYNLVVGGLSNIALNMGLYREPGYDSLA
                     DFTCAGLVVSHSYTLVARKDLPLASLAEVIAYARANPGKLNIGTSGPGTGQYIGAAII
                     AALTGVDIVKVPYKGAQPVYQDLLAGRVDLFYDNTTTTRPYVESGQVKALAVSSRERA
                     PTMPQLPTLVETGVVDLEMETWFGLFAPARTARGVIERLRAEVDRATRSAEVRSRLEM
                     GSGRSLRIAPIETEAFVRAEVAKWTRLLRRAGIEPE"
BASE COUNT          385 a          859 c          793 g          396 t
ORIGIN      
        1 cgattcccgg cacgtccggt tcctgcactg gaacgccgta aaaagacacc gcggcccgca
       61 ggtccggcac gccggccgcg acgcgccagg tcaccgcgcc gccgaagcag aacccggtca
      121 ttccgacccg gtctgcgcgc gcccaaggca gcgatctcgc atacgcgagg ccgctcttga
      181 aatcctgcac gtggcgggcg ggagccgcct tgccgaggat cccgggaatc gcgtcgaact
      241 cgtgcttcgc ggttccgccc tcgcgcgaaa gcagatccac ggcgagcccg acgtagccgg
      301 cctttgcgac gcgccgcgcg acgtcctcga tgtggcgcgt gagcccgcgg ttctcgtggc
      361 agacgagcac gatggcgtgg cgctccgttc ccgccggccg cgcgaggtat ccgtcgagcc
      421 gcacgccttc tccgggaaat tcggcgatcc cggctacgat cgagggatcg ttgggcgcga
      481 ccagcggcgc cgagctcgcg ggggccttgc cggccggcgc gacttgcgcg cgcgcctcga
      541 gcatctgcgc cgcgagcgcg gcgccggtga tgccggcgat cgccttcagc gcctcgcggc
      601 gcgtgaggcg cccctcctcg tagtcctcga cggcttcctc caccaaatag cgctgcagct
      661 cgttcatgtc catctctcct tgtagaaaaa ccggggacag accaccattt tcggtgtttc
      721 cattccctac ccatttgcgc taaagaaaat agtggtctgt ccccggtttc attacgatcc
      781 cagccaagct ctcgcctcgg cgataagctt ctcgagcgcc tcgccgcgcg cggtcgggag
      841 gttcggattc acgcgggcgc cgttcttcgt ctgcagatag gcgagatgcc ggtagccctc
      901 cggaaagcgc gccagaagcc ccgcgtcgag cgagatgctg gcgcttgggt cgaccgggtc
      961 gatgcggtcc ttctcgtaga tcaccgagcg cctcgaaatc cgccacacgc cgtcgcgctt
     1021 ctccacctgg tcgaagaagc ggccgctgca cactgcgtcg acttcgacgc cgtcgagccg
     1081 cgagcgcacg ttgatcgaca ttcgggtctg cacgatcgcg cgggcaccct tgatttccgc
     1141 cgaggtgccg cccagaaaat gctgcgagcg cgatcccttg ccgaatgaag cctcgcagga
     1201 ctcgacgaaa tcctcgaatc tcccgtcgaa ccaggtcgtg gtcatcgcgg ccccggggtg
     1261 cgcggtcgcg cgcagcctgc cccagtctcc ggtgtcgcgc gccagcgccc agtcctgcat
     1321 gagggcggag atcgcgagct ggtcctccag atccggcatg cgctcactcg ggctcgattc
     1381 ccgccctgcg aagcagccgc gtccatttgg ccacctccgc gcgcacgaac gcctcggtct
     1441 cgatgggcgc gatgcgcagg ctcctgccgc tgcccatctc caggcgcgaa cgcacttcgg
     1501 ccgagcgcgt tgcgcggtcc acctcggcgc gcaggcgctc gatcaccccg cgcgcggtgc
     1561 gcgccggcgc aaacaggccg aaccatgtct ccatttccag gtcgaccacc ccggtttcga
     1621 cgagcgtggg aagctgcggc atcgtcggcg cgcgctcgcg gctcgacacc gcgagcgcct
     1681 tcacctgacc ggactcgacg tagggacggg tggtggtcgt attgtcgtag aacaggtcga
     1741 cgcggcccgc gagcaggtct tggtacacgg gctgcgcgcc cttgtaaggc accttgacga
     1801 tgtcgacgcc ggtgagcgcg gcgatgatgg ccgcgccgat gtactgcccg gttccgggac
     1861 cgctggtgcc gatgttgagc ttgccgggat tcgctcgcgc atacgcgatc acctccgcga
     1921 ggctcgcgag cggcaggtcc ttgcgcgcga ccagggtgta agagtggctg acaacgaggc
     1981 ctgcgcaagt gaagtcggcg agcgaatcgt agccgggctc cctgtaaagt cccatgttga
     2041 gcgcgatgtt cgaaagcccc ccgacgacga ggttgtagcc gtcgggcgcg gagcgcgcga
     2101 cgagctgcgt gccgacgagc gtgccggcgc cggggcggtt ctcgaccacg aaggcctgcc
     2161 ccgtctgctc gcccagcttc tgcgccagca cacgccccac cagatcgaac ccgccgcccg
     2221 gagcggaggg aacgacgatg cgcaccgggc gcgacgggta gtcctgggcc gacgccgcgg
     2281 agaacgccga gaaggcggcc aaaacacaag ccgcgcggca caagcgtgaa ctcccaggat
     2341 tagacatggc tttcatatta gaataccgcg gcgtttttcg cacagtctcc cgatccggac
     2401 ccatcattcg caacgctatt cgcttgccgt gcg
//
LOCUS       DASPZD010000421         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_283279, whole genome shotgun sequence.
ACCESSION   DASPZD010000421 DASPZD010000000
VERSION     DASPZD010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283279"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VEM33_08140"
     CDS             complement(<1..243)
                     /locus_tag="VEM33_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYS51836.1"
                     /translation="MSIAVAVPETSRGSYREMWLITLGHSLTHWYPATFYLILPIIGK
                     ELGLSYSQIGLIMTCQYIAGAVANIPGGVLVDTVGRK"
     gene            complement(270..1295)
                     /locus_tag="VEM33_08145"
     CDS             complement(270..1295)
                     /locus_tag="VEM33_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS51837.1"
                     /translation="MRSFAVFAACVLLACSAGAQTLFDYGGADRTKRIVAAAKKEGSL
                     TMYTTFAEKDQPTLIKPFEAKYGVKVNIWRAGTDKVLQRTLAEAAARKYDVDVIHFGS
                     PEMEALSREKILQAVNSPVHKDLQPGSVPAHREWAATLLSVWVQVYNTNLIKKSDLPR
                     TYADLLDPRWKGKLGIEAKNQDWFASVVDVMGGGEKGLKFFRDLVARNGISPRTGHTL
                     LNNMVIAGEVPLALTVYNYMPEQAKKKGAPIDWFALEPAVARSNAIGVARRAPHPNAA
                     LLFYEYMLGEGQQYLVKMDYVPSNVQAPSPLRNVKILQTDPIRSLDETDKWTKLFEDV
                     VLKGGGN"
     gene            complement(1318..2412)
                     /locus_tag="VEM33_08150"
     CDS             complement(1318..2412)
                     /locus_tag="VEM33_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51838.1"
                     /translation="MTLLIDNETVARVLTPAAVRGALESAYRDLASGEAVCRPRIDIR
                     IPTRDPAKFYQWGSMEGGSTGGYFAIRMKSDVVYEREYEGARTREKYCSRPGRYCGLV
                     FLTDVETGEPLAIINDGTLQHLRVAADSAIGTDIMAREDCRALGLLGSGGMARSHVEA
                     LLEVRRIERLKVFSPTRANRERFAAEIAKRHDIECEALESPREVYRGADILAACTDSA
                     VPVIRGEWLEAGMHVVSIGGRPDDAALSRFDRTLRLGTAPAPVGRPELATADEYLGYV
                     ARPQDPRWGTNRVGRRAVTGRGGDVSFADVASGKVRGRTARDQITYSERGNIQGAQFF
                     AVAAAAYEAARREGLGRELPTDWFLQDIRD"
     gene            complement(2409..>2956)
                     /locus_tag="VEM33_08155"
     CDS             complement(2409..>2956)
                     /locus_tag="VEM33_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51839.1"
                     /translation="ASYGDLLHPRWKGKLGIEASDEDWFAETVRNLGEETGLRLFREL
                     AAKNGLSVRRGHSLLAQMVASGEVPFALTVYNFTAEQLKQQGAPLEWFMLPPAIAHGN
                     GFAILKRAPHPHAALLYYEFMIGDDGQRILAERKSVPTSRKIRTALDRTSLKIIDPVV
                     LVDQGERWAKLYEEIIVKGSR"
BASE COUNT          462 a          989 c          957 g          548 t
ORIGIN      
        1 cttgcgtccg acggtgtcga ccagcacccc gccggggatg ttcgcgacgg cgcctgcaat
       61 atattggcag gtcatgataa ggccgatctg gctgtaggaa aggcccaatt ccttcccgat
      121 gatgggcagg atcagataga aggtcgccgg ataccagtgc gtgagcgaat ggccgagggt
      181 gatcagccac atctcgcggt acgagccgcg ggaggtttcg gggacggcaa cggcgatgct
      241 catttcgctt ttcctcgccc gcgatattat cagttcccgc cgcccttcag aacgacgtcc
      301 tcgaagagct tcgtccattt gtccgtttcg tcgagcgagc ggatcggatc ggtctgcagg
      361 atcttcacgt tcctgagcgg agaaggcgcc tggacgttcg agggcacgta atccattttc
      421 accaggtatt gctgcccctc gccgagcatg tattcgtaga agaggagggc tgcgttcgga
      481 tgcggcgcac ggcgcgcgac gccgatcgcg ttggagcggg ccaccgcggg ctcgagcgca
      541 aaccagtcga tcggcgcgcc ctttttcttc gcctgctcgg gcatgtagtt gtatacggtg
      601 agcgcgagcg gcacttcgcc ggcgatgacc atgttgttga gcagcgtgtg cccggtgcgc
      661 ggcgagatgc cgttgcgcgc cacgaggtcg cggaagaact tgagcccctt ctcgcctccg
      721 cccatcacgt cgaccaccga ggcgaaccaa tcctggtttt tcgcctcgat gccgagcttg
      781 cccttccacc tggggtcgag caggtccgca taggtccggg gcaggtccga cttcttgatg
      841 agattcgtgt tgtagacctg cacccatacc gaaagcagcg tggcggccca ctcgcggtgc
      901 gccggtaccg agcccggctg cagatccttg tgcaccgggg aattcacggc ctgcaggatc
      961 ttctcgcgcg aaagcgcctc catctcgggc gagccgaagt ggatcacgtc cacgtcgtac
     1021 ttccttgccg ccgcctcggc gagcgttcgc tgcagcacct tgtcggtgcc cgcgcgccag
     1081 atgttgacct tgacgccgta cttcgcttcg aagggcttga tcagcgtcgg ctggtccttc
     1141 tcggcgaagg tggtgtacat cgtgagcgag ccctccttct tcgccgccgc gacaattctc
     1201 ttcgtgcggt cggcgccgcc gtagtcgaag agcgtctggg cgccggcgct gcaggcgagg
     1261 agcacgcagg cggcgaaaac cgcgaaggat ctcattgcat ttttcatggt ctctctctca
     1321 atcgcgtatg tcctgcagaa accagtccgt cggcagctcg cgcccgaggc cttcgcggcg
     1381 cgccgcctcg taggccgcag cggcgaccgc gaaaaactgg gctccctgga tattgccgcg
     1441 ctccgagtag gtgatctggt cgcgcgcggt gcggccgcgc accttgccgc tcgccacgtc
     1501 ggcaaagctg acgtccccgc ccctgcccgt caccgcgcgc cggcccacac ggttcgtgcc
     1561 ccagcgggga tcctgcgggc gcgcgacgta tcccagatac tcgtcggccg tggcgagctc
     1621 gggccttccc accggcgcgg gcgcagtccc cagccgcagc gtccggtcga agcgggaaag
     1681 cgccgcgtcg tcgggccgcc cgccgatcga tacgacgtgc atccccgcct cgagccactc
     1741 tccgcggatc acgggaaccg cggaatcggt gcaggccgcg agaatgtccg cgccccggta
     1801 tacctcgcgc ggcgattcga gggcttcgca ctcgatatcg tggcgcttcg cgatctcggc
     1861 ggcaaagcgc tcgcggttcg cccgcgtcgg gctgaacacc ttcaggcgct cgattctcct
     1921 cacctcgagc agggcttcga cgtgcgagcg ggccatgccg cccgagccga gcaacccgag
     1981 cgcgcggcaa tcctcccgcg ccatgatgtc ggtcccgatg gcgctgtccg cggcgacgcg
     2041 caggtgctgc agggtgccgt cgttgatgat ggcgagcggc tcgcccgttt cgacgtcggt
     2101 caggaacacc aggccgcagt agcggcccgg ccgcgagcag tacttctcgc gcgtgcgggc
     2161 gccttcgtac tcgcgctcgt acacgacgtc ggatttcatc cggatggcga aatagccgcc
     2221 ggtggaaccg ccctccatgc tgccccactg gtagaacttc gcgggatcgc gcgtcgggat
     2281 gcggatgtcg atgcgcggcc ggcagaccgc ttcgcccgag gcgaggtcgc ggtaggccga
     2341 ctccagcgcg ccgcgcaccg ccgcgggcgt gagcacgcgt gctacggtct cgttgtcgat
     2401 gagcagagtc acctgcttcc cttcacgatg atttcctcgt agagcttcgc ccagcgctcg
     2461 ccctgatcga cgagaaccac cgggtcgatg attttgagcg aggtccgatc cagcgcggtg
     2521 cggatcttcc tgctcgtcgg aacggacttg cgctccgcca ggatccgctg gccgtcatcg
     2581 ccgatcatga attcgtagta gagcaaggcc gcatgcgggt gcggcgcgcg cttgaggatt
     2641 gcaaacccgt tgccgtgcgc gatcgcgggc ggcagcatga accactcgag cggagcgcct
     2701 tgctgcttca actgctcggc ggtgaagttg tagacggtga gcgcgaacgg cacttcgccc
     2761 gaggccacca tctgcgcgag aaggctgtgc cctctgcgca cagacaggcc gttcttcgcc
     2821 gccagctcgc gaaacagtct caaccccgtc tcttccccca ggttccggac agtttcggca
     2881 aaccaatcct cgtcggaggc ctcgatgccg agctttcctt tccagcgcgg atgcaggagg
     2941 tcgccataag acgctg
//
LOCUS       DASPZD010000422         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_152990, whole genome shotgun sequence.
ACCESSION   DASPZD010000422 DASPZD010000000
VERSION     DASPZD010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152990"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VEM33_08160"
     CDS             <1..820
                     /locus_tag="VEM33_08160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS51840.1"
                     /translation="DMDRMGIDIQAVSPAPQQTYYWTEPGLGAEVSRMVNDRLAEIVA
                     KWPDRFVGLGTVPLQNVELAVIELERCVKQLGLRGVEINPNVAGRELTDPSLNLDRFF
                     AKARELDIVIFMHPIGFTQGDRLMDHYLNNLIGNPLDTTVGTSHLIFGGVMERHPGLK
                     IVLPHAGGFLGHYWARMDHGWRARPDCRTVIKKPPTSYLKKFFFDTITFDPEMLGNLI
                     DKFGAAQVLLGTDYPFDMGEEDPVGLINSVPRLPAAEKERIMGGNAARLLKIRR"
     gene            852..1916
                     /locus_tag="VEM33_08165"
     CDS             852..1916
                     /locus_tag="VEM33_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYS51841.1"
                     /translation="MEQIGVAIIGTGWCGGIRAETCAAHPLTKSLHLAEILPERLAEV
                     AKATGAKSAVADYRELLKIPEVEAVYISATPETTHFPIARDSLAAGKHVFLEKPIAME
                     LSEADELIALARKNKLKFTIGYSQRFNPKFAYVRKSIRNGTIGKPVSALVSRHITRGL
                     GKKISGRVKLSPAAMECTHDLDFVLWCLEPAKPVRVYSQANYGAMQASSGAPIADTQW
                     ITVTMDSGLSFVVGGGWSLPPGYPNFSTTWIEMVGTEGALLVDDSHRDVVLNTMEKGM
                     QLPMSTMPGERVDHTYAGPMAAETIHFLEAVAYDRPVMVTPEHARMVMEIYLAADISA
                     EKNESVSLPLKAAKSVRAAA"
     gene            2027..3163
                     /locus_tag="VEM33_08170"
     CDS             2027..3163
                     /locus_tag="VEM33_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYS51842.1"
                     /translation="MPKKNPKRIGLAIIGGGRVGLFRGEIAARHPQVDFIGLAEIKPD
                     RAKLVGEKIRADFVTDDYEKLLARPEVTAAIIATDEHLHVAPILAAVERKLPLLIEKP
                     LATGLADSERVLAAIQKSGVDAVVGYTQRFRRRWLVAKEKVRTGQLGDVSLVTSRAFM
                     NRLVALDNYERTDDPSGISPMVISGTHALDIVMWMMEAKRPVEVYARSVDKALGPLCG
                     GIDATAGVVSFEDGSLYHASISWALPKVWPGAVYSLEVGIVGTEGVLTIDDTHRDIVL
                     ATTIAQGEGYAPDAGRRVDFLGSYPPGDMALGELRGPMREETEQWLNRLAVGVTTQHA
                     TAAEAHNRLMLTKAFDLSARLKRAVPLPITAMDEKPRMGVRAAV"
     gene            3186..>3550
                     /locus_tag="VEM33_08175"
     CDS             3186..>3550
                     /locus_tag="VEM33_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51843.1"
                     /translation="MKRKLALWAALALALCPALSPAQSAYPNRSVKIIVPFAPGGASD
                     FVARIMQPRLSELLGQPIVIENRGGAAGTIGMEVAARSAPDGYTLVLGNVGSTAINPG
                     VFTNLSINNLRDFIPVTQVV"
BASE COUNT          689 a         1129 c         1141 g          591 t
ORIGIN      
        1 ggacatggac cgcatgggca tcgacatcca ggcggtgtcc ccggcgccgc agcagaccta
       61 ctactggacc gagccggggc tgggggcgga ggtctcgcgc atggtgaacg accgcctcgc
      121 ggagatcgtg gcgaagtggc cggaccgctt cgtcggcctg ggcaccgtgc cgctgcagaa
      181 cgtcgagctg gcggtgatcg agctcgagcg ctgcgtcaag cagctcggcc tgcgcggggt
      241 cgagatcaat ccgaacgtcg ccgggcgcga gctgaccgat ccctcgctca acctcgatcg
      301 gttcttcgcc aaagcgcgcg agctcgacat cgtgatcttc atgcatccga tcgggttcac
      361 gcagggcgat cggctgatgg atcactatct caacaatctt atcggcaatc ccctggatac
      421 gacggtcggc accagccatc ttattttcgg gggagtgatg gaaaggcacc cgggacttaa
      481 gatcgttctc cctcacgcgg gcggctttct cgggcactac tgggcgcgca tggaccacgg
      541 ctggcgcgcg cggcccgatt gccgcacggt gatcaagaaa ccgccgacga gctatctcaa
      601 gaaattcttc ttcgacacga tcacgttcga tcccgagatg ctgggcaacc tgatcgacaa
      661 attcggcgcg gcgcaggtgc tcctgggcac cgactacccc ttcgacatgg gcgaggagga
      721 cccggtcggc ctgatcaaca gcgtgccgcg cctccccgcg gccgagaagg aaaggataat
      781 gggaggcaac gctgcccgat tgctcaagat caggcgataa tacgcaatcg cagcagcacg
      841 ctggaggtcc catggagcag ataggcgtag cgatcatcgg caccggctgg tgcggcggca
      901 tccgtgcgga aacgtgcgcc gcccatccgc tgacgaagag cctgcacctc gcggagatcc
      961 tccccgagcg gcttgccgag gtcgccaagg cgaccggcgc caagagcgct gtcgccgact
     1021 accgcgagct gctgaagata cccgaggtcg aggccgtgta catctcggcg acccccgaaa
     1081 ccacgcactt tcccatcgcc agagacagcc tcgcggccgg caagcacgtg ttcctcgaga
     1141 agccgatcgc gatggagctc tcggaggccg acgagctgat cgccctcgca cgcaagaaca
     1201 agctgaaatt cacgatcggc tattcgcagc gtttcaaccc caagttcgcc tacgtgcgga
     1261 agtcgattcg caacggcacc atcggcaagc cggtgagcgc cctggtgagc cggcacatca
     1321 cgcgtggact cggaaaaaag atcagcgggc gcgtcaagct ctcgcccgcc gcaatggagt
     1381 gcacgcacga tctcgacttc gttctgtggt gtctcgagcc ggcgaaaccg gtgcgcgtct
     1441 attcccaggc gaactacggc gcaatgcagg cttcgagcgg cgcaccgatc gccgatacgc
     1501 aatggatcac cgtcacgatg gactcgggcc tctccttcgt cgtgggcggc ggctggagcc
     1561 tgccgcccgg ttatccgaat ttctcgacga cctggatcga aatggtcggg accgagggcg
     1621 cgctgctggt ggacgacagc caccgcgacg tcgttctgaa caccatggaa aagggcatgc
     1681 agctgccgat gtccacgatg cccggggagc gcgtcgatca cacttacgcg ggtccgatgg
     1741 cggccgaaac catccacttt ctcgaagctg ttgcgtatga ccgcccggtc atggtgacgc
     1801 ccgagcacgc gcgcatggtg atggagatct acttggcggc ggatatctcg gccgagaaga
     1861 acgaatccgt ctcgttgccg ctaaaagcgg cgaagagcgt gcgcgcggca gcgtgagcgc
     1921 tacatagtca cgaaaaccgg accctcgaaa gactcatcac ccagcccgac cccgaatgaa
     1981 tcgcgatcgt accgggtgat gagtctttcg agggtctatc actgaaatgc caaagaagaa
     2041 ccccaagcgg atcggcctcg ccatcatcgg cggaggccgt gtcggcttgt tccgcgggga
     2101 aatcgccgcg cgtcacccgc aggtcgactt catcggcctc gcggagatca aacccgatcg
     2161 cgcaaaactc gtcggggaaa aaatccgcgc cgacttcgtc accgacgact acgagaagct
     2221 gctcgcgcgc cccgaggtca cggccgcgat catcgccacc gacgagcacc tgcacgtagc
     2281 cccgattctc gccgcggtgg agcggaagct cccgcttctg atcgaaaagc cgctcgccac
     2341 ggggctcgcc gattcggagc gcgtcctcgc ggcgatacag aaatccggag tggacgcggt
     2401 ggtcggctat acgcaacgct tccgccgccg ctggttggtg gcaaaggaga aggtgcgcac
     2461 cggtcagctc ggcgacgtaa gcctggtcac ctcgcgggca ttcatgaacc ggcttgtcgc
     2521 gttggacaat tatgagagga ccgacgaccc ttccgggatt tcgccgatgg ttatctccgg
     2581 tactcacgcg ctcgacatcg tgatgtggat gatggaagcg aaacgcccgg tggaggtcta
     2641 tgcccgctcg gtggataagg cgctcggccc cttgtgcggg ggcatagacg ccacggcggg
     2701 cgtcgtgagt ttcgaagacg ggagcctcta tcacgcgtcg atttcctggg ccttgccgaa
     2761 ggtctggccc ggcgcggtgt acagcctgga agtgggcatc gtcggcaccg agggcgtcct
     2821 caccatcgac gatacgcatc gcgacatcgt tcttgcgacg acgatcgcgc aaggtgaagg
     2881 ctacgccccg gatgccggac ggagggtcga ttttctcggc agctatcctc cgggggatat
     2941 ggcgctggga gagctgcgcg gtccgatgcg cgaagaaacc gaacaatggc tgaaccgcct
     3001 ggcggtgggc gtgactacgc agcacgcgac cgccgccgag gcgcacaacc ggctgatgct
     3061 gaccaaggcc ttcgatcttt cggcgcgctt gaagcgcgcc gttccgctcc cgatcacggc
     3121 gatggacgaa aagccgcgca tgggagtgcg tgccgcggta taacgcaaga aagaggagag
     3181 agacgatgaa gcgcaaactc gccctgtggg ctgcgctcgc gctggccttg tgcccggcgc
     3241 tttcgccggc gcagagcgcc tatccgaacc ggtcggtaaa aataatcgtt ccgttcgctc
     3301 cgggaggcgc gtcggacttc gtcgcccgca tcatgcagcc gcgcctttcg gagctcctcg
     3361 gccagccgat cgtgatcgag aaccgcggcg gagcggccgg aaccatcggc atggaggtcg
     3421 cggcccgctc ggctcccgac ggctacaccc tggtcctggg gaacgtcggc tcgacggcga
     3481 tcaatccagg ggtgttcacc aatctgtcaa tcaacaacct gagggacttc atcccagtga
     3541 cccaggtggt
//
LOCUS       DASPZD010000423         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_656322, whole genome shotgun sequence.
ACCESSION   DASPZD010000423 DASPZD010000000
VERSION     DASPZD010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656322"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..595
                     /locus_tag="VEM33_08180"
     CDS             155..595
                     /locus_tag="VEM33_08180"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HYS51844.1"
                     /translation="MQPDIHVNYWAILVAVAATMVLGFLWYGPILGKAWMKEMGIPAG
                     SKPDPKVMRRGLILMLIGSFLTAFVLAHTGEVWRPSVWKAGSDQSAAIYGFLTGFWTW
                     IGFYVPLLLGGVSWENRSWKLFCINAAYYFVMLQMVAMILAFWR"
     gene            complement(601..1206)
                     /locus_tag="VEM33_08185"
     CDS             complement(601..1206)
                     /locus_tag="VEM33_08185"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYS51845.1"
                     /translation="MAASEADRPERSPLVILFAEMRGFTGMSDMLDPAIVLARVGEFV
                     SLMAKAVEAHEGAVVNILSDTVMATFTGQDDAQHAVEAAQEIQTGFATLAEAWQRDFG
                     IRAAVSQGLHCGDAVVGFAKASPTPEQLFVFGDCVSIANRLLHRARAGEFVLSETLMD
                     LATEMGVVIEAEELPPLEIPRRDPIRLFGVLLDTRLDFTSD"
     gene            1410..2063
                     /locus_tag="VEM33_08190"
     CDS             1410..2063
                     /locus_tag="VEM33_08190"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYS51846.1"
                     /translation="MAVRIGDEAPDFTAETTEGKISFHEWIGDKWAILFSHPKDFTPV
                     CTTELGYMAKLKPEFDKRNTKIIGLSIDPVSDHNKWVNDIKETQGTAVNYPMIGDPDL
                     HVAKLYDMIHPNASGGKRTAADNATIRSVFMIGPDKKVKAMLIYPMSAGRNFDEVLRL
                     LDSLQLNAKHAVATPVNWKPGQDVIIPTSVSDEEARKKYPQGYKTHKPYLRTVAQPK"
     gene            2065..2922
                     /locus_tag="VEM33_08195"
     CDS             2065..2922
                     /locus_tag="VEM33_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYS51847.1"
                     /translation="MDEHRSPAEGSGTLQVGELTVRRMGLGAMRVTTDGGDIWGPPAD
                     PRTAKRLLHRALELGINFFDTADSYGPHASESFIAETLHPYPEGLVIGTKGGVVRPGR
                     HRWDPDGRPEHLRRALDGSLKRLKLERIDLYQLHAPDPQVPFADSVGALAEAQRAGKI
                     RHLGLSNVSVEQLEAAQRIVPIASVQNQYNLEYRKSEDVLMVCERLGVAFTPWYPLGD
                     GAALKSRGVKQLARRLGVPPAQVALAWLLAHSRVMLPIPGTASIAHLEENTAAAALRI
                     PPEDLAALN"
     gene            2941..3387
                     /locus_tag="VEM33_08200"
     CDS             2941..3387
                     /locus_tag="VEM33_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51848.1"
                     /translation="MQRIAMFLAILGAVASAALIGGCKSPPTQAEMAAYDYGPRPENH
                     ERLIRDYLGPKLLDPGSAIIEFKAGPKQLFQQETALRPLAYGWGVCVWINSKNTSGVY
                     DGPFPMVFFIRDGKIVAANGGPDDNVIGWRYARTGCNELGAPFWTP"
     gene            3375..3836
                     /locus_tag="VEM33_08205"
     CDS             3375..3836
                     /locus_tag="VEM33_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51849.1"
                     /translation="MDALIEQPVRLLMGILAATSIALASLAGCQSPPLTREELAALDY
                     GPRPEDYEKIIRDHLRTRLVEPDFALIEFKAGPKPLYQKDALLRDRQHGWAVCVMIND
                     KGWRGGYEGFYPMVLYIREGKVVAANGDGLERAAGVRYAHAQCRELDYEVP"
     gene            3853..4812
                     /locus_tag="VEM33_08210"
     CDS             3853..4812
                     /locus_tag="VEM33_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51850.1"
                     /translation="MKIAPNAWAALLLAAATPLHAQDYPKGSVTLVIPLAPGDATDTS
                     ARAIADELSRELKVAIVPVNRPGAGGAVGTELVVKSKPDGYTIILTNNASLVFRSILD
                     PKTASYNPLTDLTPLGLAMRSPSILAVRGDAPYKTFAEMVEHSKKNPGSVRIGTAGAG
                     SVGDFCVLTLNSLTAAGLTTVPFTGASPAVTALLGGHVEGVVLALGTMTGHLRSGTLR
                     GIVISTRYPDFPGIPTLSELGYSEPFFGIWVGFFAPANVPAEVTRALVPALERAIRSP
                     AIGAKLRPLGMLMDYAPPEKLLAEIREEHRRVAEIAAKSGLIR"
     gene            complement(4833..5210)
                     /locus_tag="VEM33_08215"
     CDS             complement(4833..5210)
                     /locus_tag="VEM33_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51851.1"
                     /translation="MATPDLKSSELKLDLDKLYLEEIFTDRRIGTIRRLSPVARDGKP
                     DASRTVLYVGETQIMTPAGSIPIGFEIAAASLEEAAEKFGPAAREAIDRTVRELQELR
                     RQASSSIVVPQGPLSGGGKIQLP"
     gene            complement(5451..>5688)
                     /locus_tag="VEM33_08220"
     CDS             complement(5451..>5688)
                     /locus_tag="VEM33_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51852.1"
                     /translation="AKIRPQSFSGYWGDGDGTSCVMGAAFEAVGIPYRSKGLDRARYF
                     PSLTHVVAEEIFYRNDNLHQSREKISGWLKAQGL"
BASE COUNT         1043 a         1877 c         1785 g          983 t
ORIGIN      
        1 accgccaagg tttcctcgct tcgctccaag accgccaagc gcgtgcacgc ctgaagcgac
       61 ccggaagggt cccgcttgca tccgccaagg gatgctccta gcattcgaac gcggcccacg
      121 cacgcccggg ccgcgttcga attggggaga ttccatgcaa cctgacattc atgtgaacta
      181 ctgggcgatt ctcgtcgcgg tggccgccac gatggtgctc ggctttctct ggtacggccc
      241 gatcctgggc aaggcgtgga tgaaggagat gggcatcccg gccggcagca agcccgaccc
      301 gaaggtcatg cggcgcggct tgatcctcat gttgatcggc tccttcctca ccgcgttcgt
      361 gctcgcgcac accggcgagg tatggcggcc ttccgtgtgg aaggccggat ccgaccaatc
      421 ggccgccatt tacggcttcc tcaccggctt ttggacctgg atcggcttct acgtgcccct
      481 gcttctgggc ggagtgtcct gggagaaccg gtcctggaag ctgttctgca tcaacgccgc
      541 gtactacttc gtgatgctgc agatggtcgc aatgatcctg gcgttctggc gctaaaggcc
      601 tcaatcgctg gtaaagtcca gtcgcgtatc gaggagcacc ccaaaaagcc tgatcggatc
      661 gcggcgcggg atttccagcg gcggtagctc ctctgcctcg atcacgacgc ccatctcggt
      721 cgcaaggtcc atcagggtct ccgagagcac gaactcgccc gcgcgtgcgc ggtgcagcag
      781 gcggttggcg atgctcacgc agtcgccgaa cacgaagagc tgctcgggag tcgggcttgc
      841 cttcgcgaac cccactaccg cgtccccgca gtgcagcccc tgcgataccg ccgcccggat
      901 gccgaaatcg cgctgccacg cctcggcgag ggtcgcaaat ccggtctgga tctcctgcgc
      961 cgcctcgacc gcgtgctggg cgtcgtcctg ccctgtaaag gtcgccatca cggtgtcgct
     1021 gaggatgttc accacggcgc cctcgtgcgc ctcgaccgcc ttggccatca gggacacgaa
     1081 ttcgccgacg cgcgcgagca cgatcgcagg atcgagcatg tccgacatgc ccgtaaaccc
     1141 gcgcatctcc gcgaacagga tcacgagcgg agagcgttcg ggcctgtccg cttcgctagc
     1201 cgccacctcg tactctcccc tctcccgcca cgccgggttc ctccgctata agcatattat
     1261 tcaaatgtca ttaattcgcg ccgccgggcg gagtataatt tttttcatcc ccgtacgttc
     1321 aacgggagtt ctcgaattct caactcagga caggaaaccc gcaatcgcgg cgcccgggca
     1381 ttccggcccg ccaccaagac ggaggtcaaa tggctgttcg cataggcgat gaagcaccgg
     1441 acttcacggc agaaaccacc gaagggaaga tcagcttcca cgaatggatc ggagacaaat
     1501 gggcgatcct tttctcgcac ccgaaggatt tcacgcccgt gtgcaccacc gagctcggct
     1561 acatggcgaa gttgaagccc gagttcgaca agaggaacac caagatcatc gggctttcga
     1621 tcgacccggt aagcgaccac aataaatggg tcaacgacat caaggagact caaggaactg
     1681 ccgtcaacta tccgatgatc ggcgatccgg acctgcatgt cgcgaaactc tacgacatga
     1741 tccaccccaa cgcgagcggc ggaaagcgca ccgcggcgga caacgccacg atccgctcgg
     1801 tgttcatgat cggccccgac aagaaagtga aggcgatgct catctacccg atgagcgcgg
     1861 gccgaaattt cgacgaggtt ctgcgcctcc tcgattcact gcagctgaac gccaaacatg
     1921 ccgtggcgac gccggtgaac tggaagccgg gacaggacgt gatcattccg acctcggtgt
     1981 cggacgagga ggcgaggaaa aagtacccgc aagggtacaa gacgcacaag ccgtatctgc
     2041 ggactgtggc gcaacccaag tagcttggac gagcatcgga gtccagcgga ggggtcggga
     2101 acgctgcagg tcggcgagct caccgtgcgg cgcatgggtt tgggcgcgat gcgcgtgacc
     2161 accgacggcg gcgacatctg gggcccgccc gccgatccgc gcacggcgaa gcggctgctg
     2221 caccgcgcgc ttgagctcgg catcaatttc ttcgacaccg ccgacagcta cgggccgcac
     2281 gcttccgagt ccttcatcgc ggaaaccctg catccctacc ccgaggggct cgtcatcggc
     2341 accaagggcg gggtcgtgcg cccgggaagg caccgttggg acccggacgg ccgacccgag
     2401 catctgcgcc gggcgctgga cgggagcttg aagcgcctga agctcgaacg catcgacctc
     2461 tatcagcttc acgcgccgga cccgcaagta cccttcgccg actcggtggg cgcgctcgcc
     2521 gaggcccagc gcgccggcaa gatccggcac ctggggctct ccaacgtcag cgtggagcaa
     2581 ctggaggccg cgcagcggat cgttcccatt gcttcggtgc agaaccaata caatctggag
     2641 taccggaaat cggaagacgt gctcatggtg tgcgaacggc tcggcgtcgc cttcaccccg
     2701 tggtatccgc tcggcgacgg agcggcgctc aagtcgcgcg gcgtgaaaca gctcgcgcgg
     2761 cggctcggcg tgccgcccgc gcaggtggcg cttgcgtggc tgctcgcgca ttcgcgggtc
     2821 atgctgccga ttcccggcac ggcctcgatc gcgcatctgg aggaaaatac cgcagccgcg
     2881 gccctgagaa tcccgcccga ggaccttgca gcgctcaatt gaaaaggaac ggaggaacct
     2941 atgcagcgta tcgcgatgtt tctggcaatc ctgggcgccg ttgcgtcggc cgccctcatc
     3001 gggggctgta agagcccgcc gactcaggcg gagatggccg cctacgacta cggcccgcgt
     3061 ccggaaaacc acgagaggct gatacgggac tacctcgggc ccaagctcct cgacccgggc
     3121 agcgcgatca tcgagttcaa ggccggaccc aagcagctct tccagcagga aacggcgctg
     3181 cgccccctcg cctacggctg gggggtctgc gtgtggatca acagcaagaa cacgagcggc
     3241 gtctacgacg gtcctttccc catggtattt ttcatccgcg acgggaagat cgtcgcggcg
     3301 aacggcgggc cggacgacaa cgtcatcggc tggcgctacg cgcgcaccgg gtgcaacgaa
     3361 ctcggcgcgc cgttttggac gccctgatcg aacagcccgt gcgcctgctg atggggatcc
     3421 tcgccgcgac ctcgatcgcc ctggcgtcgc tcgccgggtg ccaatctcct ccgctcaccc
     3481 gcgaagagct cgcggcgctc gattacggcc cgcgcccgga ggactacgaa aaaatcatcc
     3541 gcgaccatct gagaacccgg ctcgtcgagc ccgattttgc cttgatcgag ttcaaggcag
     3601 gccccaagcc cctctaccag aaagacgcgc tcctgcgcga ccgccaacac ggctgggcgg
     3661 tgtgcgtgat gatcaacgac aagggttggc gcggaggcta tgaggggttc tacccgatgg
     3721 ttctctacat tcgcgaaggg aaagtcgtcg cggcgaacgg ggacggactg gagcgcgccg
     3781 cgggcgtccg ctacgcgcat gctcaatgcc gggagctcga ctacgaagta ccgtgatggg
     3841 agtatccaga caatgaaaat cgcgcccaac gcatgggcgg cgctgctgct cgccgcggcg
     3901 accccgctcc atgcccagga ctaccccaag gggtcggtga cgctcgtcat tccgctcgcg
     3961 cccggagacg cgacggatac ttccgcacgc gcgatcgccg acgagctttc ccgcgagctc
     4021 aaggtggcga tcgtcccggt gaaccgtccc ggcgcggggg gcgcggtcgg caccgagctg
     4081 gtcgtgaagt cgaagcccga tggctacacc atcatcctca cgaacaacgc gtcgctcgtc
     4141 ttccgctcga tcctcgatcc caagaccgcg agctacaacc cgctcaccga cctcacgccg
     4201 ctgggactcg cgatgcgctc gccgagcatc ctcgctgtgc gcggcgacgc gccctacaag
     4261 accttcgcgg agatggtcga gcactcgaaa aagaatccgg ggagcgtgcg tatcggcaca
     4321 gccggggcgg gatcagtggg agacttctgc gtgctgacgc tcaattccct caccgccgcc
     4381 gggctcacca cggtgccgtt cacgggcgcg tcgcccgcgg tcaccgccct gctcggcggg
     4441 catgtcgaag gcgtcgtact cgcgctcggc acgatgacgg gccacctgag gagcggcacg
     4501 ctgcggggca tcgtcatctc gactaggtat ccggatttcc cgggcatccc gacgctttcg
     4561 gaactcgggt actcggaacc gttcttcgga atctgggtgg gattcttcgc gccggcaaac
     4621 gtgcccgccg aagtcacgcg ggcgctggtt ccggcgctcg agcgcgccat caggtcgccg
     4681 gcgatcggcg cgaagctcag gcctctcggg atgctgatgg actatgcccc gccggagaag
     4741 cttctcgcgg agatacgcga agagcaccgg cgcgttgcgg agatcgcggc gaagtccgga
     4801 ctgatcaggt aggcctccgc gacggcgggt ctttagggaa gctggatctt cccgccgccg
     4861 ctcaaggggc cctgcggaac gacgatcgag gagctcgcct ggcggcgcag ctcctgcagc
     4921 tcgcgtacgg tgcggtcgat cgcctctctt gcggccggtc cgaacttctc ggcagcctcc
     4981 tcgagcgacg cggcggcgat ctcgaatccg atggggatgg agccggcggg ggtcatgatc
     5041 tgcgtctcgc cgacgtagag gacagtcctc gaggcgtccg gttttccatc tctcgcgacg
     5101 gggctcaggc gacggatcgt gccgatgcgc ctatcggtga agatttcctc caggtagagc
     5161 ttgtccaggt cgagcttcag ctcggaggac ttgagatcgg gcgtggccat gcgtcggctc
     5221 caagagacca aaggctgcga gcttggcctc cgcgtgttcc ggcgtcaaga ggagggatca
     5281 atcgcgtttc ttggcgaact ccgttgcagc gagcgtctcc cagtatcgca ccacggcggt
     5341 gtgatccagg cccaggccga gtgtggcctg tgcttcgctc taggcttcga ggcaaaccgt
     5401 ccagctgagt tgttcgagcg ccttcacgat gagcaggacg ccctcgtggc tcacagtcct
     5461 tgcgctttca gccaaccaga aattttctcg cgcgactgat gcaagttatc gttgcggtaa
     5521 aagatttctt cggcaactac atgcgtcaaa ctcggaaagt acctagccct gtccaatcct
     5581 ttgctgcggt atgggatgcc tacagcctca aaagccgcgc ccatcacgca actcgtaccg
     5641 tccccgtctc cccaatatcc ggagaatgat tgcggcctga tcttggcg
//
LOCUS       DASPZD010000424         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_288974, whole genome shotgun sequence.
ACCESSION   DASPZD010000424 DASPZD010000000
VERSION     DASPZD010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288974"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2550)
                     /locus_tag="VEM33_08225"
     CDS             complement(<1..2550)
                     /locus_tag="VEM33_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYS51853.1"
                     /translation="MPESQHERTPLTQLASFDALFRAGLGYNQPASPLSALGVMAAGS
                     GDTASKYVARRETPLYIPENCTQCMECIAVCPDTALPNCSQDLETILRTAVTNYVTDA
                     GERQKMLHLMPEIEKRTRDMMRAGLAKDPPPLQQIIQKVTNEVNGFSAQAKREFFDII
                     DRVPLAYQKTNAIFATPEKKNPGGGGVFSIFVSDLCKGCAACVTACGDHRALKMVQET
                     EDVNAEHETGTAFLDLLPDTPQKYLGLYNDLRPQDSKTATLRNMLMVRRNYDALVSGD
                     GACAGCGEKSILRAIAAVTEAYMRPLYHAKADRLRAKADRIEQSGEQKLAELKERSPE
                     EYDMFRQAVAHLLMGLGGEDDKDTKARIASHGPISDKDAIDAITAIMRQEAFNHRDLQ
                     PIDGRLGNGMSVMAMAAHTGCNTVYGSTPPNNPHPYPWMNSLFQDGITVGWLLGESFI
                     VDHGRRSVIPERLADALMNRQSDVINPREYYEYAHFSDAVMTDQEILELPKAWVVGGD
                     GGMGDIGYQNTSKVVLQNRPNVKALMLDTQVYSNTGGQNSDSTPMLGGNDMNVFGAAT
                     QGKNTEKKTVAETFLAGHGSPFVAQVSIANAPKLYRAILDGLEYRGTMFMQCFTTCQP
                     EHGVADDMALDQAQRVRDSRGAPEFVFNPRLGETYQETLDIKGNPSIDMDWYETKFKG
                     TGEPYRYTVAHWCATEARFRNHLKKIKQEETAALVPLENMLVRITQQDVVYRRYLVPS
                     HRSYVPDFGVFIRVQGPNGTIEYRSLSRQLVLFCVERRKAWRMLQSKAGIENREYKAQ
                     RSVLADVDAGKISSEELFARAEELMRERIKSGTGAPASARHAPRPAAAAPGA"
BASE COUNT          381 a          860 c          868 g          530 t
ORIGIN      
        1 ggccccaggc gctgccgcgg caggccgcgg tgcatgcctc gccgatgccg gcgcgcccgt
       61 cccgctcttg atccgctctc tcatcagctc ctcggcgcgc gcgaacagtt cctcgctgga
      121 gatcttgccg gcgtcgacgt cggcaaggac cgagcgctgg gccttgtatt cccggttctc
      181 gatgccggcc ttgctctgca gcatccgcca cgccttgcgg cgctcgacgc agaagagcac
      241 gagctggcgc gagagcgacc ggtactcgat ggtgccgttg ggcccctgca cacggatgaa
      301 cacgccgaag tccggcacat aggagcggtg gctgggcacc aggtaccgcc ggtagacgac
      361 gtcctgctgc gtgatgcgga cgagcatgtt ttcgagcggg acgagcgccg cggtttcctc
      421 ctgcttgatc ttcttcaggt gattccggaa ccgggcttcc gtcgcgcacc agtgggccac
      481 cgtgtaccga tacggctcgc cggtgccctt gaacttcgtt tcgtaccaat ccatgtcgat
      541 cgacggattg cccttgatgt cgagcgtttc ctggtaggtt tcgcccagcc ttgggttgaa
      601 gacgaattcg ggtgcgccgc gcgagtcgcg gacgcgctgc gcctggtcga gcgccatatc
      661 gtcggccacg ccgtgctcgg gctggcacgt ggtgaagcac tgcatgaaca tcgtgccgcg
      721 gtactccagg ccgtcgagga tcgcgcggta gagcttcggg gcattggcaa tcgagacctg
      781 cgcgacgaag ggcgacccgt gaccggcgag gaacgtctcg gcgacggtct tcttctccgt
      841 gttcttgcct tgcgtggcgg cgccgaacac attcatgtcg ttgccgccca gcatcggtgt
      901 cgagtcggag ttctgcccgc cggtgttcga gtagacctgg gtgtcgagca tcagcgcctt
      961 gacgttcgga cggttctgca gcaccacttt cgacgtgttc tggtagccga tgtcgcccat
     1021 gccgccgtcg ccgccgacca cccacgcttt cggcagctcg aggatctcct ggtcggtcat
     1081 caccgcgtcg ctgaaatggg cgtactcgta gtactcgcgc gggttgatga cgtcgctctg
     1141 gcgattcatg agcgcgtcgg ccagccgctc gggaatcacg gatcggcggc cgtgatcgac
     1201 gatgaagctc tcgccgagga gccagcccac cgtgatcccg tcctggaaca gcgagttcat
     1261 ccacgggtac ggatgcggat tgttgggcgg ggtcgagcca tacacggtgt tgcagcccgt
     1321 atgcgcggcc atcgccatca ccgacatgcc gttgccgagg cggccgtcga tcggctggag
     1381 gtccctgtgg ttgaacgcct cctgcctcat gatggccgtg atcgcgtcga tggcgtcttt
     1441 gtcggagatc gggccgtggc tggcgatccg tgccttggtg tccttgtcgt cttcaccgcc
     1501 gaggcccatg agcaaatggg cgacggcctg gcggaacatg tcgtactcct ctgggctccg
     1561 ctccttcaat tccgccagct tctgctcgcc gctctgctcg atcctgtcgg ccttcgccct
     1621 caaccgatcg gccttcgcgt ggtagagcgg acgcatgtac gcttcggtga cggcggcaat
     1681 cgctcgcagg atgctcttct cgccgcagcc ggcgcacgcg ccgtcccccg acaccagcgc
     1741 gtcgtagttc cgccgcacca tcagcatgtt gcgcagcgtg gcggtcttcg agtcctgcgg
     1801 gcgcaagtcg ttgtagagcc ccaggtactt ctgcggcgta tcgggcagca ggtccaggaa
     1861 cgcggtgccg gtttcgtgct ccgcgttcac atcctcggtt tcctgcacca ttttcagtgc
     1921 ccggtgatcg ccgcaggccg tgacgcaggc cgcgcagcct ttgcaaaggt cggacacgaa
     1981 gatcgagaag acaccgcccc cgccgggatt cttcttctca ggcgtcgcga aaatcgcgtt
     2041 cgtcttctgg taggccaacg gcaccctgtc gatgatgtcg aaaaattcgc gcttggcctg
     2101 cgccgagaag ccgttgacct cgttggtcac cttctggatg atctgctgga gcggcggcgg
     2161 gtctttcgcg agacccgcgc gcatcatgtc gcgggtgcgc ttttcgatct ccggcatgag
     2221 gtggagcatc ttctgtcgct cgccggcgtc ggtcacatag ttggtgaccg ccgtccgcag
     2281 gatcgtctcg aggtcctgcg agcagttggg gagcgcggtg tccggacaga ccgcgatgca
     2341 ctccatgcac tgcgtgcagt tttccgggat gtacagcggc gtttcgcgcc gggcgacgta
     2401 cttggacgcg gtgtcgccgc tgcccgcggc catgacaccg agcgccgaca gcggcgaggc
     2461 cggctggttg tagccaagcc cggcgcggaa cagcgcgtcg aacgaggcga gctgcgtcag
     2521 cggcgtccgc tcgtgctgac tctcgggcat cgggtgcgat cggcatccgg agccgcaccc
     2581 gtctccgttc tcgtgaacca ccgggaggag cgcctgacca cgaagcgtcg accgatctg
//
LOCUS       DASPZD010000425         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1253709, whole genome shotgun sequence.
ACCESSION   DASPZD010000425 DASPZD010000000
VERSION     DASPZD010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253709"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="rsmB"
                     /locus_tag="VEM33_08230"
     CDS             <1..1094
                     /gene="rsmB"
                     /locus_tag="VEM33_08230"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HYS51854.1"
                     /translation="RGLLLTALAEIAARPQSAHAVVHQAVEAAVLLGHSRAKGLVNAV
                     MRNFQRRMPELLSEIEATESGRYRHPQWWIDALRLAYPHEWESVLLESNRHPPMVLRV
                     NRRRLPAKAYLEKLERAGIPARELGQDALLLEKPCRVERLPGFAAGEVSVQDAGAQRA
                     APLLDVRDGMRVLDACAAPGGKTGHLLELAECELLAVDADGVRARRIAENLSRLKLSA
                     KVVVGDCRKPEDFSEGRPFDRILLDAPCSASGVVRRHPDIKWLRRKADVSEFGRAQAE
                     LLEALWRVLAPEGKLLYATCSVFPEENGAQVDNFLLRHPGAEMLPLPGLIEGGEGVPR
                     PFDGQILPGADSDGFFYALLKKNTAQTAE"
     gene            1103..1663
                     /locus_tag="VEM33_08235"
     CDS             1103..1663
                     /locus_tag="VEM33_08235"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HYS51855.1"
                     /translation="MRRFVAALALVLATPALAQPIQVNNAGLEIAETRYFLNADFHLD
                     LTAPLLEALKSGVSLGFLVEFQLTRPRWYWFDEKTASEKLELRLSYLPLAQQYRLSSG
                     TLYQNFPTLSEALEALGRVHGWAVLGQDQVDNGRTYVAAVRLRLDPAQLPTPFRVSAV
                     TNREWTLTSDWKRFRFTPLAPVQEAR"
     gene            1660..>2000
                     /locus_tag="VEM33_08240"
     CDS             1660..>2000
                     /locus_tag="VEM33_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HYS51856.1"
                     /translation="MTYVLIASAALGGILLFLLAAATANSPLFAEHYPLLLGLNAAIA
                     LALLGLVVYQLTILARQRRAKVFGSLLTFRVLVMFALVGVVPGLLVYTVSLQFLAKSI
                     ESWFDVRVERAL"
BASE COUNT          311 a          626 c          659 g          404 t
ORIGIN      
        1 ttcgtgggtt gctgctcacc gcgctcgcgg agattgccgc ccggccgcag tccgcgcacg
       61 cggtagtgca tcaggcggtg gaggcggcag tcctgcttgg gcactcgcgc gcgaagggac
      121 tcgtcaacgc ggtgatgcgc aacttccagc gccgcatgcc cgagctgctc tcggagatcg
      181 aggctaccga gtctggtcgt taccgccatc cgcagtggtg gatcgacgcg ttgcgcctgg
      241 cctacccgca cgagtgggag tccgtcctgc tggagtcgaa ccggcacccg cccatggttt
      301 tgcgggtaaa ccggcggcga ctcccggcga aggcgtatct ggagaaactg gagcgtgctg
      361 gaattcccgc gcgggagctg ggacaagatg cgctgctgct cgaaaagcct tgccgcgtcg
      421 agcggcttcc gggatttgca gcgggcgagg tctcggtgca ggatgccggc gctcagcgag
      481 cggcgcccct tctggatgtt cgcgacggga tgcgagttct ggatgcctgt gcggcgcccg
      541 gtggcaagac cggacacctg ctcgagcttg ccgagtgcga gcttctcgca gtcgacgcgg
      601 acggcgtgcg ggcgcggcgt atcgccgaaa atctctccag actgaagctc tccgcgaaag
      661 tcgtggtcgg cgattgtcgc aagccggagg atttctcgga ggggaggcct ttcgaccgaa
      721 ttcttctgga tgcaccttgc tcggcatcgg gcgtggtgcg acgccatccc gacatcaaat
      781 ggctgcgtcg gaaggccgat gtatccgagt tcggtcgcgc gcaggcggaa ttgctggaag
      841 ccttgtggcg ggtactcgcc cccgaaggta aattgttgta cgccacgtgc tcggtgtttc
      901 ccgaggagaa cggcgcccag gtggacaatt ttcttctgcg gcacccggga gctgaaatgc
      961 ttccgctccc agggctcatc gagggtggcg aaggggtccc gcgccctttc gacggccaga
     1021 tcctgccggg cgccgatagc gatggtttct tctacgcctt gctgaaaaaa aacactgccc
     1081 aaactgcgga atgaagacaa gcttgcgtcg attcgttgct gcgctcgcac tggtcctcgc
     1141 tacgccggcg ctcgcccagc ccatacaggt caacaacgcc gggttggaga tcgccgagac
     1201 gcgctatttc ctgaacgctg attttcacct tgatctgacc gcgccgctcc tggaggccct
     1261 gaaaagcggc gtatcgctcg gcttcctcgt ggagttccag ctgacgcggc cgcgatggta
     1321 ctggttcgac gagaagaccg cctcggaaaa gctggagcta cggctttcct acctcccgct
     1381 tgcgcaacag taccggctgt cgagcgggac gctgtatcag aacttcccca ctctttccga
     1441 ggcgctggaa gcactcggca gggttcacgg ctgggcggtg ctgggacagg atcaggtcga
     1501 taacggccgg acctatgtcg cagcggttcg gctgcgcctt gatccggctc agctgccaac
     1561 gcccttccga gtgagtgccg tcaccaaccg cgagtggacg ctcacctccg attggaagcg
     1621 gtttcggttt accccgctcg ccccggtgca ggaggcccga tgacctacgt gctcatcgcc
     1681 agcgccgcgc tcggcggcat tttgctgttc ctgctggccg cggcgaccgc caactcgccg
     1741 ctcttcgccg agcactatcc gctgctgctc ggtctgaatg cggcgattgc tctggctctc
     1801 ctcgggctcg tcgtgtacca gttgacgatc ctcgcgcggc agcgccgcgc aaaagtgttc
     1861 ggttcgctgc tgacgtttcg ggtgctcgtc atgtttgcgt tggtgggagt ggtccccggg
     1921 ctcctcgtct atacagtgtc gctgcagttc ctcgcgaaga gcattgaatc ctggttcgac
     1981 gtgcgcgtcg agcgcgcgct
//
LOCUS       DASPZD010000426         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_44114, whole genome shotgun sequence.
ACCESSION   DASPZD010000426 DASPZD010000000
VERSION     DASPZD010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44114"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..917)
                     /locus_tag="VEM33_08245"
     CDS             complement(15..917)
                     /locus_tag="VEM33_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYS51857.1"
                     /translation="MSPRTYAPPPSILALLVLLTLGWGVNWPIMKVVMAEMPPLHFRV
                     LCLAAGAAGLFAIARANRLPIRVPSGAWPRLIAITLFNVAAWNVLAVYGVTFMESGRA
                     AILAYTFPVWGVLLGVWVLREPVTGSRLTGVVLGVLGMLVLLGDEIHAVGRSPTGALL
                     LIASAISWAVSTAIMKRWPVDLPTTSFTGWQMLLALVPILFGAMLFEEGSFSPLTLST
                     WPFWGLVYNALVSSIFCNWAWIKIATHAPISVSSLSTLMIPVVGVFSGMLLLGEHPQW
                     SDLAALVLVVAALAVVLLPQRLAA"
     gene            1204..1746
                     /locus_tag="VEM33_08250"
     CDS             1204..1746
                     /locus_tag="VEM33_08250"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HYS51858.1"
                     /translation="MNFTPEQIATTNKAGIEAILGLAHTQFTAFERLSALYFNAAKAA
                     FEEGVGHAKALLNAKDPQEYLNLNAAATQPTMEKAIAYSRSVYEVAAQTNGELAKFLE
                     AQAAELNKNLIGLLDKVSKNAPAGSDVAVQAVKSALAAANTAYDSFNKVAKQATEIAE
                     ANFAATTTPARETPRKRATA"
     gene            complement(1855..2607)
                     /locus_tag="VEM33_08255"
     CDS             complement(1855..2607)
                     /locus_tag="VEM33_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HYS51859.1"
                     /translation="MGTGAIIIGDEIIRGKREDKHFAKLIEILRVRGMRLDWCKYLGD
                     KPDLITATLKRTMASEDIVFSFGGIGATPDDHTRQCAADAAGVELTLHPDAEAAIRSR
                     TDMVVTPQRLKMGEFPAGSRIIPNSYNKIPGFSMGDHHFVPGFPVMAWPMVEWVLDQR
                     YAHLFHRTPEAEASVIVYDLPESIVTPLMIEVEQRYRGLKSFSLPSMGEAGVRRHIEL
                     GVRGAPEEVTPAIERMKLGVAALGGRWEEKRQ"
     gene            complement(2609..3355)
                     /locus_tag="VEM33_08260"
     CDS             complement(2609..3355)
                     /locus_tag="VEM33_08260"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HYS51860.1"
                     /translation="MNAILNALARAFVSLLHPKMLWLMVWPVIVALVLWVTLAVLYWG
                     EAAHWIAAQLHQWPPYEWAVSVWPLKLVAAWFGWILLLLLFVPAVLITAVLIISIVSM
                     PTMVAHVGGRDYPGLVRRKGGTFAGSLWNAFAALMLFALLFAVTLPLWLVPLLWPVLP
                     IALFGYFNQRVFRYDALAEHATATEIAEIVRRHRGGLFLLGVALALVGHVPVLGLLMP
                     VYGGLAFIHYGLERLGELRSIPIEGAAARV"
     gene            complement(3381..>4986)
                     /locus_tag="VEM33_08265"
     CDS             complement(3381..>4986)
                     /locus_tag="VEM33_08265"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HYS51861.1"
                     /translation="FFAHNRGLVREIEQLEHKSRRPDVLVDEALIHAFYDSLVPEDIH
                     NGADFDRWRRDAEAKNPKLLFLKRGDLMRHEAAGITTAQFPHRLEMAGQSFALEYHHE
                     PGSPRDGLTLTVPLLALNQIDAAKCDWLVPGLAREKATRLAKSLPQKLRHQLGPLPEF
                     VDAFLAANQPADAPLAQAIARHARREVNLAVPLDAFRPETLPAHLSMNFRVVDEHGRQ
                     LAMGRNLAQLRAELGDAAGEQFAELAQTQVPGKKVTGWDFGDLEEVAEVRRGSQTLIG
                     YHALVDHRDAVSLEVFDSADKARAEHRAGLRRLFMLQLKEQARYIEKSLPGMQATAMQ
                     FTALGSGDELRSQLLAAAFDRACMQDPLPRTRAEFARRCDEARSRVSLLAQEIARLAG
                     TILAEQASLQKKLQQMSKAFPAPCRDVQEYVGRLLPKGFIERTPYERLQHFPRYLKAA
                     GLRLDKLRADPQRDARLAAELAPLLGQWQREQLKQAKSGEPDPRLEQFRWLLEELRVQ
                     LFAQELKTSVPISVKRLSKMWQTIQR"
BASE COUNT          993 a         1621 c         1552 g          820 t
ORIGIN      
        1 ctgaacgtgc cggattacgc cgccagccgc tgcggcagga gcacgaccgc cagagcggcg
       61 acgaccaaga cgagcgcggc caaatcgctc cactgcggat gctcgcccaa caacaacatc
      121 ccgctgaaaa cgcccacgac cgggatcatc aacgtggaga gcgatgaaac gctgatcggc
      181 gcatgggtcg caatcttgat ccacgcccag ttgcagaaga tcgagctgac cagcgcgttg
      241 tagacgagcc cccagaaggg ccaagtcgag agcgtgagcg gcgaaaagct cccttcctcg
      301 aatagcatgg ccccgaagag aataggcacc agggcgagca gcatctgcca gccggtaaag
      361 gaagtcgtgg gcaggtcgac gggccagcgc ttcatgatcg cggtgctgac cgcccagctg
      421 atcgcggaag cgatgagcag cagggctccg gtcggcgagc gcccgaccgc gtggatctcg
      481 tccccgagaa gcaccaacat ccccaggacg cccagaacca cgcccgtgag ccggcttccc
      541 gtcaccggct cgcgcagaac ccacaccccg agcagcacgc cccacacggg aaacgtataa
      601 gcgaggatcg cggcacgccc cgattccatg aacgtgacgc cgtagaccgc gagaacattc
      661 caggcggcca cgttgaaaag cgtgatagcg atcagcctcg gccaggcgcc cgaagggacg
      721 cgaatcggca agcggttggc ccgcgcaatc gcgaaaaggc ccgcagcccc ggccgcaaga
      781 cacagcacgc ggaagtgcag cggcggcatt tcggccatca ccaccttcat gatcggccag
      841 ttgacccccc agccgagggt gagcaggaca agcagggcca ggattgatgg cggcggtgca
      901 taggtacgcg ggctcacgat tcgctcactt tccggccctt ccggagaaaa ggctcggatc
      961 atttaaaaac aaagagttgg aggtaagtgt ctactggctc ccaggacgac gatctcgacg
     1021 taacccttta tttatttgca gctttcactt ctcgccttgg aaaatgtagt gcgccgcaca
     1081 aattgttctt gacatcgatt gatatccttg tagaatccat tatgttgcag tgcaataata
     1141 aaggaacttg tcgcacataa ttttcaagct caaacgcagc ccttttttct aacggagatg
     1201 atcatgaact tcacgcccga acaaatcgcc accacgaaca aggctggcat cgaggccatc
     1261 ctgggtctcg cccatacgca gttcaccgct ttcgagcgcc tgtccgcgct gtatttcaat
     1321 gcagccaagg ccgcatttga ggaaggcgtc ggccacgcta aggcgctgct caacgccaaa
     1381 gacccccagg aatacctcaa cctgaatgcc gcggcaaccc agccgacgat ggaaaaagcc
     1441 atcgcctact cgcgtagcgt atacgaagtc gccgctcaaa cgaacggcga gttggccaag
     1501 ttcctcgaag cgcaagccgc cgagcttaac aagaacctga tcggtctcct cgacaaggtt
     1561 tcgaagaacg cgccggccgg atccgacgtc gccgtccaag ctgtgaagtc tgcgctcgcc
     1621 gcagccaaca ctgcctatga cagcttcaac aaggtcgcca agcaggccac ggagatcgcc
     1681 gaggcgaact tcgcagcgac tacgacgcct gccagggaaa ccccgaggaa gcgcgcgacg
     1741 gcgtaatttg caggttttcg cagcccacac ccccttctcc tcctcccctc tgtgggcgcc
     1801 ctaaagccct ggcgtaagcc agggcttttt gtttgcacgc aaaaaccctg ggttttactg
     1861 acgcttttcc tcccacctgc cgccgagcgc ggcgaccccc agcttcatcc gctcaatagc
     1921 tggcgtgact tcttccggtg caccccgcac cccgagttct atatggcggc gcacgcctgc
     1981 ctcgcccatg ctgggcaggc tgaacgactt caagccgcgg tagcgctgtt ccacttcgat
     2041 catgagcggc gtgacgatag attccggaag gtcgtagacg atgacggaag cctcggcttc
     2101 cggcgtgcgg tgaaacagat gcgcgtagcg ttggtccagc acccactcga ccatgggcca
     2161 ggccatcact gggaaacccg gcacgaaatg gtgatcgccc atcgaaaagc cggggatctt
     2221 gttgtatgag ttcggaatga tgcgcgagcc cgcggggaat tcgcccatct tgaggcgctg
     2281 cggcgtgacc accatgtcgg tgcgcgagcg gatcgccgcc tccgcatcgg gatgcaacgt
     2341 cagctccacg cccgccgcgt ccgcggcgca ctggcgagta tgatcgtcgg gtgtcgcgcc
     2401 gatgccgccg aagctgaaga cgatgtcttc cgatgccatc gtgcgcttga gcgtggcggt
     2461 gatcaggtct ggcttgtcgc ccagatattt gcaccagtcg agcctcattc cgcgcacgcg
     2521 caggatctcg atcagcttgg cgaaatgctt atcctcgcgt tttccgcgaa tgatctcgtc
     2581 accgattatg atcgccccgg ttcccatctc acacccgcgc cgccgcaccc tcgatcggta
     2641 tgctgcgcag ctcgccgagg cgttcgaggc cgtaatggat gaaagcgagc ccgccgtata
     2701 ccggcatcaa gagtccgaga acggggacat gaccgacgag cgcgagcgcg acgcccagaa
     2761 gaaacagccc gccccgatgg cgccggacga tctcggcgat ctcggtggcg gtcgcgtgct
     2821 ccgcgagagc gtcgtagcgg aagactcgct ggttgaaata accgaaaaga gcgatgggca
     2881 ggaccggcca cagcagcgga accagccata gcggcaaagt gacggcaaag agcaacgcaa
     2941 acagcatgag cgcggcgaag gcattccaga ggctgccggc gaacgtgccc cccttccggc
     3001 ggacgagccc cgggtagtcg cgcccgccga catgagcgac catcgtcggc atcgaaacga
     3061 tgctgatgat cagcaccgcc gtgatcagaa ccgcgggcac gaacaacagc agcaggagga
     3121 tccagccgaa ccatgccgca acgagcttga ggggccagac cgagacagcc cattcgtaag
     3181 gcggccactg gtggagctgt gccgctatcc aatgcgcggc ctcgccccag tagagcaccg
     3241 ccagcgtcac ccatagcacc agcgcgacga tcactggcca taccatgagc cagagcatct
     3301 tcggatgcag caaactgaca aacgcccgcg cgagggcatt gagtatcgcg ttcatatggg
     3361 ccgcccgatg cggtgaaaaa tcagcgctga atagtttgcc acatcttcga cagtcgcttg
     3421 accgaaatcg gcacggaggt cttcagctcc tgggcgaaaa gctgtacgcg caattcctcg
     3481 agcagccagc ggaactgctc caaccgggga tcgggctctc cggacttcgc ctgcttcagc
     3541 tgctcgcgct gccattgccc gagcagcgga gcgagctccg ctgcgaggcg cgcgtcgcgc
     3601 tgaggatccg cgcgcagctt gtccaggcgc aggccggccg ccttcaggta gcgcgggaaa
     3661 tgctgtaacc gctcgtaggg cgttcgctcg atgaatcctt tgggcagcag gcggccgacg
     3721 tattcctgca catcccggca gggagccgga aacgccttgg acatctgctg cagctttttc
     3781 tggaggctgg cctgctcggc gaggatcgtt ccggcgagtc gggcgatctc ctgggcgagc
     3841 aggctgacgc gcgagcgcgc ttcgtcgcag cgtcgcgcga actcggcgcg cgtgcgcggc
     3901 agggggtcct gcatgcacgc ccggtcgaac gcggcggcga gcaactgcga gcgcaactcg
     3961 tcgccgctgc ccaaggcggt gaactgcatc gccgtcgcct gcatccccgg aaggctcttt
     4021 tcgatgtagc gagcctgctc cttcagctgc agcatgaaga ggcggcgcag gccggcgcgg
     4081 tgctccgcac gcgccttgtc cgccgagtcg aacacctcga ggctcaccgc gtcgcggtgg
     4141 tcgaccaggg cgtggtagcc gatcagggtc tgcgagcccc gccggacttc cgcgacttcc
     4201 tcaagatcgc cgaagtccca tcccgtgact tttttccccg gaacctgggt ctgcgcgagc
     4261 tcggcgaact gctcgccggc cgcgtcgccc agctccgcgc gcagctgcgc caggttgcgg
     4321 cccatcgcga gctgccggcc gtgctcgtcg acgacacgga aattcatcga taggtgcgcg
     4381 gggagcgtct ctgggcgaaa agcgtcgagc ggcacggcga ggttcacttc gcgtcgcgca
     4441 tgccgggcga tggcctgagc gagcggtgcg tcggcgggct ggttcgccgc gaggaaggcg
     4501 tcgacgaact cgggcagggg accgagctgg tgccgcagct tctgcggcag cgacttggcg
     4561 aggcgtgtcg ccttctcccg cgcgagaccc ggcaccagcc aatcgcattt cgcggcgtcg
     4621 atctggttga gcgcgaggag cggcacggtg agcgtcaggc cgtcgcgggg cgaaccgggc
     4681 tcgtgatggt actcgagggc gaagctctgc cccgccatct ccagccggtg cggaaactgg
     4741 gccgtcgtga tgccggccgc ctcgtggcgc atcaggtccc cgcgcttgag gaagagcagc
     4801 ttcggatttt ttgcttccgc atcgcggcgc cagcggtcga agtccgcgcc gttgtggatg
     4861 tcctcgggca cgaggctgtc gtagaacgcg tggatcagcg cttcgtccac cagcacgtca
     4921 ggccggcgcg acttgtgctc gagctgctcg atctcgcgca ccagcccgcg gttgtgcgcg
     4981 aaaaag
//
LOCUS       DASPZD010000427         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1253832, whole genome shotgun sequence.
ACCESSION   DASPZD010000427 DASPZD010000000
VERSION     DASPZD010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253832"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VEM33_08270"
     CDS             <1..988
                     /locus_tag="VEM33_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS51862.1"
                     /translation="NPSPALDESAGRSARLVADAKREGSLSLYASLAEKDLIRLVSEF
                     ERRYGIKVNVWRSGKNNVLRRAVTEARAGRFDVDVVHNPSPEMEQLHRERLLQEVRSP
                     FQAQLIPEAVAPHREWAGPRVYIFVQAYNTNRVKAEELPKTFKDLLDPRWKGRIAIEA
                     KEQEWFFTLVREMGGEEGLKFFRDLVATNGLTVRNGNALLNNLVVAGEIPLALTLYSY
                     LPEQSRRSGAPVGWIALAPTVAYTDGIGVMKRAPHPNAAVLFYDFVLSDGQALLAELN
                     HVISHRRNEPYLRKFDLKFIDVDAVLADHDRWTRIFEDTIYGRAAPAPTASH"
     gene            992..2275
                     /locus_tag="VEM33_08275"
     CDS             992..2275
                     /locus_tag="VEM33_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYS51863.1"
                     /translation="MLTAERNELLTRVGRGTPVGELFRRYWLPALLATELPEPDCAPV
                     RVKLLGECLVAFRDSSGRLGLIEEFCAHRGVSLWFGRNEENGIRCPYHGWKYDVTGQC
                     VDLPSEPEESGMRKNIRLGSYPAIELAGVIWAYLGPADRKPEEPAYEWTRVPPAHRYV
                     SKRLQESNYLQAMEGALDSVHSGFLHRHSVADDPLLKRDPASLAMLLADRHPKIVPTG
                     SPAGLYVATRRDVGTDRYYWRVTQWLMPCFSFFPPYGDNPYGGHAFVPIDDERCWTFS
                     IDYHPARALAAGEREAAHAGRGIHVPLIEGSFIPAANRRNDYLIDREAQKSKKSFSGV
                     PGVGVQDAAVQESMGAIQDRTREHLVSSDNGIVKTRRLLMDAAGAVARGDAPPGVAPA
                     AQGARAVSMVAPRELSLPEAVAMAQKDPAQTVSTA"
     gene            2272..>2599
                     /locus_tag="VEM33_08280"
     CDS             2272..>2599
                     /locus_tag="VEM33_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51864.1"
                     /translation="MKKRALRALLAAASLAAVQPAAAQDSRGVLAELAASEGAGRDQR
                     LLEGARREGFLSLYTSFPPEDVAALNAAFEKKYGVKVRAWRAASEKVFQRTVAEAQAG
                     RDEVDIV"
BASE COUNT          436 a          887 c          868 g          408 t
ORIGIN      
        1 caatccgtct ccggcgctcg acgaaagcgc cgggcgctcg gcgcgcctcg tcgccgacgc
       61 gaagcgcgag ggttcgctgt ccttgtacgc atccctggcg gagaaggacc tgatccgcct
      121 cgtctccgag ttcgaacgcc gctacggaat caaggtcaac gtctggcgct ccggcaagaa
      181 caacgtcttg cggcgggccg tcaccgaggc gcgcgccggc cgcttcgacg tggacgtcgt
      241 gcacaacccc tcgccggaaa tggagcagct gcaccgcgag cgcctgcttc aggaagtccg
      301 ctcgccgttc caggcgcagt tgattcccga ggccgtcgcg ccgcatcgcg aatgggccgg
      361 cccccgcgtc tacatcttcg tccaggccta caacaccaac cgggtcaagg cggaggagct
      421 gccgaaaacg ttcaaggacc tgctcgatcc gcgctggaag ggacggatcg cgatcgaagc
      481 gaaggagcag gaatggttct tcacgctggt ccgggagatg ggcggggagg aaggtctcaa
      541 gttcttccgc gacctggtcg cgaccaacgg cctgaccgtg cgaaacggca acgccttgct
      601 caacaacctc gtcgtcgcgg gggaaattcc gctcgcgctc accctctaca gctatctgcc
      661 ggagcagtcg cggcgcagcg gcgcgccggt cggctggatc gcgctcgcgc caacggtcgc
      721 ctacaccgac ggaatcggcg tcatgaaacg cgcaccgcac ccaaacgcgg cagtgctctt
      781 ctacgacttc gtcctctccg acggccaggc gctgctcgcc gagctgaacc acgtcatctc
      841 gcatcgccgc aacgagccgt atttgagaaa attcgacctg aagttcatcg atgtcgacgc
      901 ggtgctcgcc gatcatgacc gctggacccg gatcttcgag gacacgatct acgggcgcgc
      961 agccccggcg cccacggcgt cgcactaggc catgctcaca gccgagcgca acgaattgct
     1021 gactcgcgtc ggccggggaa ctcccgtcgg ggagctcttc cgccgctact ggctgcctgc
     1081 gctgctcgcg acggagctgc cggagccgga ttgcgcgccg gtgcgcgtga agctgctcgg
     1141 cgaatgcctc gtcgcctttc gcgacagcag cgggcggctc gggcttatcg aggagttttg
     1201 cgcccaccgc ggggtgagcc tgtggttcgg gcgcaacgag gaaaacggaa tccgctgtcc
     1261 ctaccacgga tggaagtacg acgtgaccgg ccagtgcgtg gacctgccct cggagccgga
     1321 ggagtccggc atgcgcaaga acatcaggct cggttcgtat cccgccatcg agctcgccgg
     1381 ggtgatctgg gcctatctcg gtccggcgga caggaagcct gaggagcccg cctacgaatg
     1441 gacccgggtg ccgcccgccc atcgctacgt ctccaagcgc ctccaggaat cgaattacct
     1501 gcaggcgatg gaaggggcgc tcgattcggt tcattcgggt tttctgcacc gccactcggt
     1561 cgccgacgat cccctgctca agcgcgatcc ggcgagcctc gcaatgctcc tcgccgaccg
     1621 gcatccgaaa atcgtgccga cgggttcgcc tgccgggctg tacgtcgcga cgcgccgcga
     1681 cgtggggacg gaccgctact actggcgggt cacgcaatgg ctgatgccgt gcttcagctt
     1741 ctttcctccc tacggggaca atccctacgg cggccacgcc tttgtcccga tcgacgacga
     1801 gcgctgctgg acgttcagca tcgactatca cccggcccgg gcgctcgcag cgggcgagcg
     1861 cgaagccgcg catgccgggc gcggcattca tgtgccgctg atcgagggct cgttcatccc
     1921 cgccgcgaac aggcgcaacg actatctgat cgaccgcgag gcgcagaagt cgaaaaagag
     1981 cttttccggc gtgcccggtg tcggcgttca ggatgccgcc gttcaggaaa gcatgggagc
     2041 gatccaggac cgcacccggg agcacctcgt ctcctccgac aacggcatcg tcaagacccg
     2101 ccgcctgctg atggacgcgg cgggcgcagt ggcgcgcggg gacgcgccgc cgggcgtggc
     2161 tcccgcggcg caaggcgccc gcgcggtatc catggtcgcg ccgcgcgagt tgagccttcc
     2221 cgaagcggtt gcgatggcgc agaaggaccc ggcgcaaacg gtgtcgacgg catgaagaag
     2281 cgtgctctgc gcgcgctgct cgcagccgcg agtctcgctg cggttcagcc cgctgccgcg
     2341 caggactcga ggggcgtgct cgccgagctg gccgcgagcg aaggggccgg gcgtgaccaa
     2401 cgcctgctcg agggcgcgcg ccgcgagggt ttcctcagcc tgtacacgtc gtttcctccc
     2461 gaagacgtcg ccgcgctgaa cgcggcgttc gaaaaaaagt acggcgtgaa agtccgcgcc
     2521 tggcgcgccg cctcggagaa ggtgttccag cgcacggtcg ccgaggccca ggcggggcgc
     2581 gacgaagtcg atatcgtgg
//
LOCUS       DASPZD010000428         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_667784, whole genome shotgun sequence.
ACCESSION   DASPZD010000428 DASPZD010000000
VERSION     DASPZD010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667784"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..990
                     /locus_tag="VEM33_08285"
     CDS             445..990
                     /locus_tag="VEM33_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070849.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HYS51865.1"
                     /translation="MPRIAIPKREDAPAESKPILDNVDKMLGFVPNLHRLMSISPNAL
                     SGWATLMGSLAKTLDVKTRDGIALAVSEADGCDYCLAAHSFIAGNMAKVPADEIALNR
                     EGRSSDPKRQAAVAFAKALIETRGKVSDAQFAAVRDAGWTDANIVEMIALTAQFLLTN
                     FMNNAVQTPIDFPEVSPTKAA"
     gene            1033..1395
                     /locus_tag="VEM33_08290"
     CDS             1033..1395
                     /locus_tag="VEM33_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HYS51866.1"
                     /translation="MSRRSARPGVKRVYEPSEASDGTRVLVDRIWPRGLTKEHASVDV
                     WLKDIAPSAGLRTWFGHDPNRWREFHKRYFEELRANHTAVEHLTDLVSTGKVTLLFGA
                     HDTERNNAVALADFLAAH"
     gene            complement(1392..1889)
                     /locus_tag="VEM33_08295"
     CDS             complement(1392..1889)
                     /locus_tag="VEM33_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYS51867.1"
                     /translation="MTNSNDDGAPRPQAEVAGDLDGAGTLNGRAGASVFSIGGPDGRG
                     GTTVGANPYELLSASLAACTAMTIRLQARRRKIPLSHVEVAVSYHHSGDGGRDSFERS
                     VRLEGNLGNEERAQLMRAANMCPIGRTLGLSADIRTNDNVGVGPAASYDDDLSELPIP
                     YIDPD"
     gene            complement(2029..2211)
                     /locus_tag="VEM33_08300"
                     /pseudo
     CDS             complement(2029..2211)
                     /locus_tag="VEM33_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2343..>2541)
                     /locus_tag="VEM33_08305"
     CDS             complement(2343..>2541)
                     /locus_tag="VEM33_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51868.1"
                     /translation="LTPGDLILLISWKDAAAAQAYEDASAPNDNARVRKVRIVRDYGK
                     YDRREAPQYYADAKGGETLHA"
BASE COUNT          451 a          809 c          819 g          462 t
ORIGIN      
        1 ggtcgcgctc cggcaaatgc ggatgaagcg tgcagccgat atgctcgccg cgaggacatt
       61 ttcagttgag cagatcgcgc gggcggtcgg ttataggagc tgcagcagct ttctccgtgc
      121 ctttcgccaa gtgcatggat atgtcccggc agaatctgag ggtgaagggc gctccgcgcc
      181 ggcagctctt ccccggtcag cgcggcgagt cgggcgcagc tgaggacgaa gtccgggaca
      241 catggcatta gtgcgccggt cggtgctcag cgcatgacct gctgttatcg attgcatgat
      301 caagaaacat gatcgccgcg gcgcgacgcg ccctagtctt cgaggcagtc cagtggccac
      361 cgctggctga gacggtcggc gtcagcgacg ccctgcatcg tcaccaaccg aataatcgtt
      421 tgcggtctac gaaaggagga tcaaatgccc cgtattgcca tcccgaaacg cgaggacgcc
      481 cccgcggagt ccaagccgat cctcgacaac gtcgacaaga tgctaggctt cgtgcccaac
      541 ctgcaccgcc tcatgtcgat cagcccgaac gcgctttccg gctgggctac cctgatggga
      601 tcgcttgcga agacgctgga cgtcaagacg agggacggga tcgcgctggc ggtgtcggag
      661 gcggacgggt gcgactactg cttggccgcg cacagcttca tcgcgggcaa catggccaaa
      721 gtcccggcag acgaaatcgc gctgaacagg gaaggccgct cgagtgatcc caagcgccag
      781 gctgcggtcg ccttcgcgaa agcgctcatc gagacgcgcg gcaaagtttc cgacgcccag
      841 tttgcggcgg tcagggatgc ggggtggaca gacgccaaca tcgtcgagat gatcgcgctg
      901 actgcccagt tcctgttgac caacttcatg aacaatgcgg tgcagacccc gatcgatttt
      961 cccgaggtca gtcccacgaa ggcagcctaa cgcgagatgc ctcctttggg gaggggaggg
     1021 ctggagaatc caatgtctcg acggtccgcg cggcctggtg tcaagcgcgt ctacgagccg
     1081 tccgaggcct cggatggcac gagggtgctc gtcgaccgca tctggccgcg cgggctcacg
     1141 aaggagcatg ccagcgtcga cgtgtggctg aaggacatcg ccccgagcgc cggcctcagg
     1201 acctggtttg gccatgaccc gaaccgctgg cgcgagtttc acaagcgata cttcgaggag
     1261 ctccgcgcca atcacaccgc agtcgagcac ctgacggatc ttgtatcgac gggcaaagtc
     1321 acgctgctct tcggcgcgca cgacaccgag cgcaacaacg ccgtggcgct cgcggacttt
     1381 ctcgcggcac actagtcggg gtcgatgtac gggatcggaa gctcgctcaa gtcgtcgtca
     1441 tagcttgccg ccggcccgac gccgacgtta tcgttcgtgc ggatgtcggc gctgaggccg
     1501 agcgtccgcc cgatgggaca catgttcgca gcccgcatca gttgggcgcg ctcctcgttg
     1561 ccaaggtttc cttcgagtct gacggagcgc tcgaaggagt cgcggccgcc atccccgcta
     1621 tggtggtacg atacggctac ctcgacgtgg gaaagcggga ttttccgcct tcgcgcctgc
     1681 agccgtatcg tcatcgccgt acaagcggcg agcgaagcgc ttagcaactc gtaggggttc
     1741 gcccccaccg tcgtgccgcc cctgccgtcg gggccgccga tcgagaacac cgatgcgccc
     1801 gcacggccgt taagcgtgcc ggcgccatca aggtcgccgg ccacctcagc ctgtgggcgg
     1861 ggagctccat cgtcgttcga attcgtcatc gtgctttcct ttcagatttc gacagggctc
     1921 ttgcccggcc cggtcagagt gggtcgcggc agtcgatcgg tttacacggc atctttgcgg
     1981 gaaactccgc atcggcgcag tctcacgcgc tctgatgacg atgagcgcgt aagcgctgat
     2041 cacccactgg acgacagcga aggacgcttc gagcgaccgc ccaatcgaag gcagcgagac
     2101 cgctacgatg ttcgagccga acacgatgag ggacgagacg agcgacgccg ttagcagagc
     2161 ccggaatttc cgcgatgcct cggcgcctcg ctcgtccact gcgctcgtca tgccggccgg
     2221 tctccttgat cgtgtttcgt cgctgtcacc tcgccagagc gaccttggct ggtccacggc
     2281 tcgcgcctgg gtgaacgcag ggtcgaactc gcagcaggtc ggccctcccc gatggagctg
     2341 tgtcaggcgt ggagggtctc gccgcccttg gcatctgcgt agtactgagg cgcctcgcga
     2401 cggtcgtatt ttccgtagtc gcgcacgatc cgcactttcc ggaccctcgc attgtcattt
     2461 ggcgcggaag cgtcctcgta cgcctgcgcc gcagcggcat ctttccagga tatgagcaga
     2521 atcaggtcgc cgggcgtgag t
//
LOCUS       DASPZD010000429         4618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_662192, whole genome shotgun sequence.
ACCESSION   DASPZD010000429 DASPZD010000000
VERSION     DASPZD010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662192"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="VEM33_08310"
     CDS             <1..1493
                     /locus_tag="VEM33_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS51869.1"
                     /translation="TALHDRPNLMRAPPPRDTDVRREAEEELRRLAAERGEPPDEGAG
                     PTLIIDREESAGRRETEEIAEILKGVERTTSRIWVVTRELRVLALAGSLRREEGAAEE
                     TLIRRVLGLLIPRPSEDFDDAIADDALATGREIAGALQGRPGFRLRNTPDGKAVVISA
                     AHPIWNGDEVAGAVVVEESTNPILSVRSRALERLLVLTLAAFAVAAAVLIWFATRIST
                     RIRRLRDEAETAIDGRGRLAHLVTAQDAGDEIGDLSRSFSAMLGKLSQHHAYLESLAS
                     RLSHELRTPIAVVRSSLENLKLAPADARVYIDRAEEGLRRLGTILTRMSEATRLEQGL
                     ASFERERFDLAAVVSGCVEGYRLAYPEKPFDLRRPQERAVTLGSPDLAAQLLDKFVAN
                     AVDFAATGKRDEPIRISLSVANGFIELAVENKGPPLPEEMRGKLFESMVSVRGERRGE
                     AKSVEPHLGLGLYIARLIAEFHGGEVHARNLPSGEGVAVSATFRSA"
     gene            complement(1530..2921)
                     /locus_tag="VEM33_08315"
     CDS             complement(1530..2921)
                     /locus_tag="VEM33_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HYS51870.1"
                     /translation="MQRTVLIPLIVACSLFMENMDSTVIATSLPAIAVDIGESPLALK
                     LALTSYLVGLAVFIPISGWVADRLGSRTVFASAIVVFVAGSLLCAVSSTLAAFVAARF
                     LQGIGGAMMVPVGRLVLLRAVPKNELVAALNYLTIPALLGPVTGPALGGAITLYFHWR
                     WIFLINLPIGVLGLYLVLRHIPNVKEPELPPLDLAGFALSGVGLSVLMLGLSSLGGHL
                     LPGKITAACIILGALALAAYPWHARRTAHPVIDLSLLRLPTFHTGVIAGSLFRIGVGA
                     TPFLLPLLLQVGFGMNPLQSGLLTCATAVGAMFMKTLTTIILRQFGFRKVLTVNALVA
                     SAAIGVFGLFTAATPHIVIFLVLLASGCVRSLQFTALQAISFAEVTRETMSQASSIAS
                     MAQRLAQSLGVALGAYALQVASLAQGHSDVVSADFAPAFVAVALLSAMSLFFHAALPA
                     DAGAEVSGHGRGR"
     gene            2984..4501
                     /gene="glpD"
                     /locus_tag="VEM33_08320"
     CDS             2984..4501
                     /gene="glpD"
                     /locus_tag="VEM33_08320"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HYS51871.1"
                     /translation="MDSEHYDLLIIGGGINGAGIARDAAGRGLKVLLVEQNDLASATS
                     SASTKLIHGGLRYLEYFEFRLVAEALAERETLLRIAPHIVWPLEFVLPHESHLRPAWM
                     IRAGLFLYDHLGHRMLGFGSRSSLPMSKGVRLGGEGYGAGLKPEFERGFLYYDCWVDD
                     ARLVVLNARSAAAHGATILTRTRFVSARREGGEWAANLDGEGGSRRVRASAIVNAAGP
                     WVRQVLDEALKIRLSATVRLVKGSHIVVPRIHEERHAFILQNPDRRVIFMIPYERQFT
                     LVGTTDVPVTRDDYRAKISRKEIEYLCEAASRYARKPITPGMVVWSYSGVRPLYDDGS
                     ENPSEVTRDYHLLLDAGAEPGAAPLLSVFGGKITTYRKLAEQAMEKLSVRFPGKPAWT
                     HAEPLPGGNLDGRTFRGLLLDYRRRHPRLPKLWLTRVLRRHGALAGEIIGDAREERAL
                     GTNFGGGLYERELEYLVRNEWAREAEDVLWRRTKCGLHMTAAEKIQVRAWLAAKV"
BASE COUNT          782 a         1514 c         1660 g          662 t
ORIGIN      
        1 ccacggccct ccacgaccgc ccgaacctta tgcgcgcgcc tccgccaaga gacaccgacg
       61 tgcgccgaga agccgaggaa gagctgcggc gccttgccgc ggagcgcggc gagccgcccg
      121 acgagggcgc cgggcccacg ctgatcatcg accgcgagga gtccgccggg cggcgcgaaa
      181 ccgaggagat cgccgagatt ctaaaaggcg tggagcgcac cacctcgcgc atctgggtgg
      241 tgacgcgcga gctgcgcgtg ctcgcgctcg ccggcagcct gcggcgcgag gagggtgcgg
      301 ccgaggagac tctcatacgg cgcgtgctcg ggctcctgat cccgcgcccg agcgaggatt
      361 tcgacgacgc catcgccgac gacgcgctcg ccacgggccg cgagatcgcc ggcgccctgc
      421 agggccgtcc gggattccgc ctgcgcaaca cgcccgacgg caaggcggtg gtgatctcgg
      481 ccgcgcaccc gatctggaac ggcgacgagg tcgcaggcgc ggtcgtcgtc gaggaaagca
      541 ccaacccgat cctctcggtg cgcagccggg cgctcgagcg gctgctcgtc ctgacgctcg
      601 cggcgttcgc ggtggctgcg gcggtgctta tctggttcgc tacacggatc tcgacgcgca
      661 tccggcggct gcgcgacgag gccgaaaccg cgatcgacgg ccgcgggcgc ctcgcgcatc
      721 tcgtcaccgc gcaggacgcg ggcgacgaga tcggcgacct ctcgcgcagc ttctcggcga
      781 tgctcgggaa gctctcgcag caccacgcgt atctcgagag cctggcgagc cggctctcgc
      841 acgagctgcg caccccgatc gcggtggtgc gctcttcgct ggaaaacctg aagctcgccc
      901 cggcggacgc gcgcgtctac atcgaccgag cggaagaggg cctgcggcgg ctggggacga
      961 tactcacgcg catgagcgag gcgacgcgcc tggagcaggg gctcgcttcg ttcgagcgcg
     1021 agcgcttcga cctcgccgcg gtcgtctcgg gctgcgtcga gggctaccgc ctcgcctatc
     1081 cggaaaagcc cttcgacctg aggcgaccgc aggaaagggc ggtgacactg ggttctcctg
     1141 accttgcggc gcaactgctc gacaagttcg tcgcgaacgc cgtggatttc gcagccaccg
     1201 gcaagcggga tgagccgatc cggatttccc tttccgtcgc gaacggcttt atcgagctcg
     1261 ctgtcgagaa caaggggccg ccgctgccgg aagagatgcg cggaaaactg ttcgaatcga
     1321 tggtctcggt gcgcggcgag cgacgcggcg aagcgaagag cgtggagccg cacctcggcc
     1381 tgggcctcta catcgcgcgg ctcatcgcgg aattccacgg cggcgaggtg catgcgcgca
     1441 atctcccctc gggggagggg gttgcggtga gcgcaacgtt ccgttccgcc taagcgtcgc
     1501 ttttcggagc cggcaagcaa atccggccgc tacctgcccc tgccgtgtcc cgacacttcg
     1561 gcgcccgcat cggcggggag cgcggcgtgg aagaagagcg acatcgcgga aagcagcgcc
     1621 accgcgacga aggccggtgc gaaatcggcg ctgacgacat cgctgtgccc ctgtgcgagg
     1681 ctggcgacct gcagcgcgta ggcgccgagc gccacgccga gactctgcgc gaggcgctgc
     1741 gccatgctgg cgatgctcga ggcctggctc atggtctcgc gcgtcacctc ggcgaacgag
     1801 atcgcctgca gagcagtgaa ctgcagcgag cgcacgcagc cggaggcgag cagcaccagg
     1861 aagatcacga tgtgcggcgt cgcggcggta aaaagaccga atacgccgat ggcggcggat
     1921 gcgacgagtg cgttgaccgt gagcaccttg cggaatccga attgcctgag gatgatcgtg
     1981 gtcagcgtct tcatgaacat cgcgccgacg gcggtcgcgc aggtgagcag tcccgattgc
     2041 agtggattca ttccgaagcc gacctgcagc aggagcggca gcagaaacgg cgtcgcgccc
     2101 acgccgatgc ggaacaggct ccctgcgatg accccggtgt ggaaggtcgg cagcctgagg
     2161 agcgagaggt cgatcaccgg atgagccgtg cgccgcgcgt gccacgggta ggcggccagc
     2221 gcgagcgcgc ccaggatgat gcaggccgcg gtgatttttc cgggaaggag gtggccgccg
     2281 agggaagaga gtcccagcat cagcaccgac agcccgactc ccgagagcgc gaatccagcg
     2341 aggtcgagcg gcggcaattc cggctccttc acgttcggga tgtgacgcag cacgaggtag
     2401 agccccagca cgccgatcgg cagattgatc aggaagatcc agcgccagtg aaagtacagc
     2461 gtgatcgccc cgccgagtgc cggcccggtc acgggcccga gcaacgccgg aatggtgagg
     2521 tagttgaggg cggcgacgag ctcgttcttc ggcacggcgc gcagcagaac gaggcgcccg
     2581 accggcacca tcatcgctcc gccgatgccc tgcaggaagc gcgcggcgac gaaggcggcg
     2641 agcgtgctcg acacggcgca caggagcgac ccggcgacga acaccacgat ggcgctcgca
     2701 aacacggtgc gcgatccgag gcggtcggcg acccagccgc tgatcgggat gaacaccgcg
     2761 agaccgacga gataggaggt cagcgcaagc ttgagggcga gcggactctc cccgatatcg
     2821 accgcgatgg ccgggagcga tgtcgcgatc accgtcgagt ccatgttctc catgaacagc
     2881 gagcaggcga cgatgagggg aatgaggacg gtgcgctgca ctgcgcgtat tctgaggcaa
     2941 gtgcgccgcg gagcggaaaa tcccgcgtag aatccactcc agcgtggact ccgagcacta
     3001 cgacctcctg atcatcggcg gcggcatcaa cggcgcggga attgcgcgcg atgccgccgg
     3061 gcgcggcctg aaggtgctgc tggtcgagca gaacgatctc gcctcggcga cctcgtcggc
     3121 ctccaccaag ctgatccacg gcggattgcg gtatctcgag tatttcgagt tccgcctggt
     3181 cgccgaggcg ctcgccgagc gcgaaacgct gctcaggatc gccccgcaca tcgtctggcc
     3241 gctcgaattc gtgctgccgc acgaatcgca cctgcggccc gcgtggatga tccgcgccgg
     3301 cctcttcctc tacgatcatc tcggccaccg catgctcggc ttcgggagca ggagctcgct
     3361 gccgatgtcg aagggcgtgc gcctcggcgg cgaaggctac ggcgcgggct tgaagcccga
     3421 gttcgagcgc ggcttcttgt actacgactg ctgggtggac gacgcgcgcc tggtggtgct
     3481 caacgcgcgc tcggcggccg cgcacggcgc gaccatcctg acccgcacgc gcttcgtgtc
     3541 ggcgcggcgc gaaggcgggg agtgggccgc gaacctggac ggcgagggcg gcagccgcag
     3601 ggtgcgcgcg agcgcgatcg tgaacgccgc cgggccctgg gtgaggcagg tgctcgacga
     3661 ggcgctcaag atcaggcttt ccgcgacggt gcgcctggtg aagggcagcc acatcgtcgt
     3721 gcctcgcatc cacgaggagc gccacgcctt catcctgcag aaccctgata gacgcgtgat
     3781 attcatgatt ccctacgagc ggcagttcac gctcgtcggc acgaccgacg tgccggtcac
     3841 gcgcgacgac taccgggcga agatctcgcg caaggagatc gaataccttt gcgaggcggc
     3901 gagccgctac gcgcggaagc ccatcactcc cggcatggtg gtgtggagct attcgggcgt
     3961 gcgcccgctc tacgacgacg gcagcgagaa tccgtcggag gtgacgcgcg attaccacct
     4021 gttgctcgat gcgggagcgg agccgggcgc ggcgccgctg ctctcggtgt tcggcggcaa
     4081 gatcaccacg taccggaagc tcgccgagca ggcgatggaa aagctctccg tccgctttcc
     4141 cggcaagccc gcctggacgc acgcggagcc cctgccgggc gggaacctcg acggacggac
     4201 ctttcgcggg ctgctcctcg actaccgcag gcgccatccg cgcctgccga agttgtggct
     4261 cacgcgcgtg ctgcgcaggc acggggcgct cgccggggag atcatcgggg acgcgcgcga
     4321 ggagcgcgcg ctcggcacga acttcggcgg cggcctgtac gagcgcgagc tcgaatacct
     4381 ggtgaggaac gaatgggcgc gcgaggccga ggacgtgctg tggcggcgca ccaagtgcgg
     4441 cctgcatatg accgcggctg agaaaatcca ggtgcgcgcc tggctcgcgg cgaaggtctg
     4501 atcggtggag tggtggctcg gctatgccgc agtcggggcg gtcgtcgggt tcttcgctgg
     4561 aatgctgggg atcggcggcg gggcgatcat ggtgccgctt ctcgtctggt tcttcgag
//
LOCUS       DASPZD010000430         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_411203, whole genome shotgun sequence.
ACCESSION   DASPZD010000430 DASPZD010000000
VERSION     DASPZD010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411203"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1897)
                     /locus_tag="VEM33_08325"
     CDS             complement(242..1897)
                     /locus_tag="VEM33_08325"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HYS51872.1"
                     /translation="MNLPGTETPLATDANRGYRDLHEHLEELKKRGLLLTIDRPVDKD
                     SELHPLVRWQFVGGMDEAERKAFLFTNIVDGRGRKYDIPVVVGAIAANRAIYSAGMGA
                     PLSEIQAKWDRAIAHPIPPREIEHAACHEIVHKGEALQGEGRGLDRLPIPISTPGFDS
                     APTLTATNVITRDPETGVQNMGTYRAALKSSDRLVVRMATRVGGAGGYLHYVKYQKLG
                     KKTMPCAIVLGCPPYVAFMGPQKLPVGVDEFTVAGGLAGSPINVVRAKTVDLLVPAES
                     EIVIEGLIDTEYCEPEAPFGESHGHVALEEYNMPMRVTAITQRSNPVIPSYISQVAPS
                     ESSVIKRVAYEPLFLAHLREALGIRGVKSVALHEPLNGLLRVSVIACEKGMPRTEVWR
                     ALRGAAFFKGDMGKITIAVTDDIDPDNSDALLWAMAYRMNPAEDVQTLPHRGQGHGPK
                     REHGEEEDSTLLMDATMKGDMPPLALPKREYMERARKIWEELGLPKLHPQAPWFGYSL
                     GDWLPQWDEAAKRAIEGRYADNGKISEKQRKKGVKPETKFRPE"
     gene            complement(1894..>2276)
                     /locus_tag="VEM33_08330"
     CDS             complement(1894..>2276)
                     /locus_tag="VEM33_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51873.1"
                     /translation="DLDGMVEILAPVIPQIKSGNLRALGVTFDRRFAGLPEVPTLAEA
                     GVPGYEASAWNGVAAPARTPRAVIERLNREISAAVAVPELKQRLQDLGVDARASSPEA
                     MRELLISETAKWKAVVERAKIEKQ"
BASE COUNT          334 a          761 c          757 g          424 t
ORIGIN      
        1 gaccacgtac aacccgggct tttccggatc gccgacgagc accgcttggt tgatgccggc
       61 ggcgccgctc ggatcgcgcc atttgagctg atcgggcgcc ttgtacgcga gcgcagcggg
      121 gttgaggtcg gcggcgaggg ccgttcctgc agcaaatatc aagagcagtg caccagataa
      181 tgcgtgaaga gacttcgcga acatatcgac cccctttgga aaaccggcga cggagtcagg
      241 attactccgg cctgaacttg gtctcgggct tgacgccttt cttcctctgt ttctcgctga
      301 tcttgccgtt gtcggcgtag cggccttcga tcgcgcgctt cgccgcttcg tcccattgcg
      361 gcagccagtc accgagcgaa tagccgaacc acggcgcctg cgggtgcagc ttcggcaggc
      421 cgagctcctc ccagattttc cgcgcgcgct ccatgtattc gcgcttgggc agcgcgagcg
      481 gcggcatgtc gcccttcatc gtggcgtcca tcagcagggt cgagtcctct tcctccccgt
      541 gctcgcgctt ggggccatgg ccctggccgc gatgcggcag cgtctgcacg tcctcggcgg
      601 gattcatgcg ataggccatc gcccagagca gcgcatcgga attgtccggg tcgatatcgt
      661 cggtgacggc gatggtgatc ttgcccatgt cgcccttgaa gaaggccgcg ccgcgcagcg
      721 cgcgccacac ctcggtgcgc ggcatgcctt tctcgcaagc aatcacgctg acgcgcagaa
      781 ggccgttgag cggctcgtgc agcgcgacac tcttgactcc ccggatgccg agcgcctcgc
      841 gcaggtgggc gaggaacagc ggttcgtagg cgacgcgctt gatcacgctc gattccgaag
      901 gcgcgacctg gctgatgtag gagggaatca ccgggttact gcgctgcgtg atcgcggtca
      961 cgcgcatcgg catgttgtat tcctcgagcg cgacgtggcc gtgcgattct ccgaaaggcg
     1021 cttcgggctc gcagtactcg gtgtcgatca ggccctcgat cacgatctcg ctctcggcgg
     1081 gaacgagcag gtcgacggtc ttcgcgcgca ccacgttgat cggcgagccg gcgaggccgc
     1141 ccgcgaccgt gaactcgtca acgcccaccg gcagcttctg cggtcccatg aaagccacgt
     1201 agggcgggca gccgagcacg atggcgcagg gcatcgtctt cttcccgagc ttctggtatt
     1261 tcacgtagtg caggtagccg cccgctccgc cgacgcgcgt cgccatgcgc acgaccaggc
     1321 ggtcggacga tttgagcgcc gcgcgatagg tccccatgtt ctgcacgccg gtctcggggt
     1381 cgcgggtgat cacattggtc gcggtgagcg tgggcgcgga atcgaagccc ggtgtcgaga
     1441 tcgggatcgg caggcgatcg aggccgcggc cctcgccctg cagcgcctcg cccttgtgca
     1501 cgatctcgtg acaggcggcg tgttcgatct cgcgcggcgg gatggggtgg gcgatggcgc
     1561 ggtcccactt cgcctggatc tcgctcaaag gcgcgcccat gccggccgag tagatcgcgc
     1621 ggttcgcagc gatcgcgccg accacgacgg gaatgtcgta cttgcggccg cggccgtcga
     1681 cgatattcgt aaacaggaaa gccttgcgct cggcttcgtc catcccgccg acgaactgcc
     1741 agcgcaccag cggatgcagc tccgagtcct tgtcgaccgg gcgatcgatc gtcagcagca
     1801 gcccgcgctt cttcagctcc tcgaggtgct cgtggagatc gcggtagccg cggttcgcgt
     1861 cggtcgcgag cggcgtttcc gtgccgggca ggttcattgc ttctcgattt tcgcgcgttc
     1921 cacgaccgct ttccacttgg cggtctcgga gatgagcagc tcgcgcatcg cttcggggga
     1981 gctcgcgcgc gcgtcgaccc ccaggtcctg caggcgctgc ttcagctcgg gcacggcgac
     2041 cgccgcgctg atctccctgt tgaggcgttc gatcacggcc cgcggcgtcc tggccggcgc
     2101 ggcgacgccg ttccacgcgg acgcctcgta accgggaact cccgcctctg cgagggtcgg
     2161 cacctcgggc aatccggcga agcgccgatc gaaagtcacg ccgagcgccc tcaggttccc
     2221 gctcttgatc tgcgggatga cgggcgcgag gatctcgacc atcccgtcca ggtcct
//
LOCUS       DASPZD010000431         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_406361, whole genome shotgun sequence.
ACCESSION   DASPZD010000431 DASPZD010000000
VERSION     DASPZD010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406361"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..922
                     /gene="zapD"
                     /locus_tag="VEM33_08335"
     CDS             179..922
                     /gene="zapD"
                     /locus_tag="VEM33_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HYS51874.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERANHFVAEEDPRAHHAALMTL
                     FEILEVAGRAELKSDLLQELERQKQILLGFRNNPQIAEGVLQQVISDIERSSAALFAM
                     TGKIGQYLRENDWLMSIKQRSGIPGGVCEFDLPSYHFWLHREGARRGTDLGTWISPLL
                     PIRDASSIVLKLLREGGKPVKHVAHRAAFSQMLGGKMSQMVRIRLPRESQFIPEISAN
                     RYALNVRFSTFGTRVCEADVEFELTFCNL"
     gene            927..1136
                     /locus_tag="VEM33_08340"
     CDS             927..1136
                     /locus_tag="VEM33_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HYS51875.1"
                     /translation="MAKARFVSCPRCGAQVAWSPGNPYRPFCSARCKLIDLGGWAMER
                     YRVPVEESKDAPGAEEQDEGSGNRD"
     gene            complement(1163..1540)
                     /locus_tag="VEM33_08345"
     CDS             complement(1163..1540)
                     /locus_tag="VEM33_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYS51876.1"
                     /translation="MTKILCVEDSEDSLFSLHKRLALAGFEVKIAMNGAEGVDWAKTL
                     LPDLIVMDLRLPGLDGWEATRRLKGQPETKHIPIVVVTSDTSEKSREKAFAAGCDEYE
                     TKPVNFEKLVGKIHSLLSRGAKA"
     gene            complement(1655..2620)
                     /locus_tag="VEM33_08350"
     CDS             complement(1655..2620)
                     /locus_tag="VEM33_08350"
                     /inference="COORDINATES: protein motif:HMM:NF006530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HYS51877.1"
                     /translation="MSAPVDVAVAVLIRSDGAALLAQRPETKVYSGYWEFPGGKIEPG
                     EPVPEALRREVREELGIEVERAYPWITRVFVYPHAKVRLHFYRVYAWRGEPRAVEHQA
                     IAWQRPDAIELDPLLPANGPVIRSLMLPSEYAITHAGALGFEPFLSKLEARLRGGLKL
                     IQMREKTLAPRAVAEFARRVTALARAHGAKVLVNSDAALAREIDADGVHLTAEQLRSA
                     HARPDFPLCGASCHSIEELRRAEALGVDFVVLGPVRATPSHPNAVPLGWERFREIAAG
                     AAVPVYALGGIVPSDLEQGMSCGAHGIAMVRGSWGSGEKPQDSTI"
     gene            complement(2633..3517)
                     /locus_tag="VEM33_08355"
     CDS             complement(2633..3517)
                     /locus_tag="VEM33_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS51878.1"
                     /translation="MSELERLIKRGEALLERVETLLPPALRPVDWHQSVAFRWRKTSR
                     RGFIQPVANLHRIRLSDLHGVDSQKALIEQNTRQFVERRTANNALLTGARGTGKSSLV
                     KAVLDKFHPQGLRLIEVEKHDLIDLPDIVDQVAGRPERFILFCDDLSFELSEAAGYKA
                     LKVVLDGSITATSENLLIYATSNRRHLMPEYMQENLETKHLGEEIHPAETVEEKISLS
                     ERFGLWVSFYPFDQDAYLDIAGHWLRELGCSEAEILKARQEALQWALLRGSRSGRVAW
                     QFARDYAGRNPGDAKARR"
     gene            complement(3526..>4015)
                     /locus_tag="VEM33_08360"
     CDS             complement(3526..>4015)
                     /locus_tag="VEM33_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HYS51879.1"
                     /translation="GPAASSRRDLLKLEVAVSEVARHLAQAIVRDGEGATKFITVCVE
                     RGRTETECANVAYSIAHSPLVKTAFSASDPNLGRLLAAIGNAGVRDLEVGRVELYLDE
                     VLVSKDGGRAPAYREEDGKRVMAKSEITVRVVLNRGLAATMVWTCDLSHDYVTINAQY
                     RT"
BASE COUNT          712 a         1283 c         1277 g          743 t
ORIGIN      
        1 aacacccgag gcgctcgaac gccaggtgtc gaatctgaat gagaaatatc tcacgcttgc
       61 cgcaagctcc aagacaacgt catagcgcac gcccgttgtc aattcgcggc ggatcgggga
      121 gaatccggcg aggctccgcc gggatcaatc gcctaccctc ccggccgcgg gtgtcaaggt
      181 gatcacctac gagtatccgc tcaacgaacg aatccggacc ctgctgcgtc tggaagacct
      241 gttcgagcgc gccaatcact ttgtagccga ggaagatccg cgcgcgcacc acgccgccct
      301 gatgacgctg tttgaaatcc tggaagtcgc cggtcgcgcg gaactcaagt cggaccttct
      361 gcaggagctc gaacggcaga aacagatcct gctcgggttt cgcaacaacc cccaaatcgc
      421 ggaaggcgtg ctgcagcagg tgatatccga catcgagcgc tcgagcgcgg cgcttttcgc
      481 gatgaccggc aagatcggcc agtacctgcg cgagaacgat tggctgatgt cgatcaagca
      541 gcgcagcggg atccccggcg gggtttgcga attcgacctt ccctcctacc atttctggct
      601 gcaccgcgaa ggcgccaggc gcggaaccga tctcggcacc tggatctccc ctttgctgcc
      661 gatccgcgat gcgtcgtcca tcgtgctgaa acttctgcgc gaaggcggaa agcccgtgaa
      721 acacgtcgcc catcgcgccg ctttctcgca aatgctcggc ggcaagatgt cgcaaatggt
      781 gcgcatccgg ctgccgcgcg aatcgcagtt catccccgaa atcagcgcaa accgctacgc
      841 cctgaatgtc cgtttcagca ctttcggcac acgggtatgc gaggcggacg tcgagttcga
      901 gctgactttc tgcaatctgt gagaccgtgg ccaaggcgcg cttcgtgagc tgcccgcgct
      961 gcggcgcgca ggtggcgtgg tccccgggga atccttaccg gccgttttgt tcggcgcgtt
     1021 gcaagctcat cgatctcggc ggctgggcga tggaaaggta ccgggttccg gtggaggaaa
     1081 gcaaggatgc gccgggtgcg gaagagcagg acgaaggatc agggaacagg gattgatctt
     1141 gccttgcacc ctgctctctc ggtcatgctt ttgcgccccg gctgagaagc gagtgtattt
     1201 tcccgacgag tttctcaaaa ttgaccggtt tcgtttcgta ttcgtcacag ccagcagcga
     1261 acgccttctc gcggcttttc tctgacgtat ctgaggtgac gacgacgatc gggatgtgtt
     1321 tcgtttccgg ctggcctttc agccggcgcg tcgcttccca gccgtcgagc cccggaagtc
     1381 tcaggtccat gacgattagg tcgggcagga gcgttttcgc ccaatcgaca ccctccgcgc
     1441 cattcattgc gatcttgacc tcgaatcctg cgagggccaa gcgcttgtgt agcgagaaca
     1501 ggctgtcctc gctgtcctca acacagagaa tcttcgtcat cgcgtccttt cctttccgga
     1561 aggtgctccg cgcatgacgg acgcctcaag caaagcgcgg caacccggta cggtatcttt
     1621 acgtttgaag gaagacaagg acgcccgatc cggatcagat ggtagagtcc tggggctttt
     1681 cgccgcttcc ccaactgccc cgcaccatgg caatgccgtg cgcgccgcag ctcatgccct
     1741 gttcgagatc ggaaggaacg atcccgccga gcgcgtagac cggcacggcg gcgcccgcag
     1801 caatttcccg gaatcgttcc cagccgagcg ggactgcgtt cggatgcgac ggtgtcgcgc
     1861 gcaccggtcc cagcacgacg aagtcgactc ctagcgcctc cgcgcggcgc agctcctcga
     1921 tggaatggca cgaagcgccg cacaacggaa aatcggggcg ggcgtgggcg ctgcgcaatt
     1981 gttcggcggt gagatgcacg ccgtcggcgt cgatttcgcg cgcgagcgcg gcatccgagt
     2041 tgaccaggac tttcgcgccg tgggcgcggg ctagcgcagt cacgcgtcgc gcgaactccg
     2101 ccacggcccg aggcgcgagg gttttttccc gcatttggat gagcttgagc ccgccgcgca
     2161 gcctcgcctc gagcttcgaa agaaatggct cgaaaccgag cgcgccggcg tgcgtgatgg
     2221 cgtactcgga gggcagcatc aggctgcgta tcaccgggcc gttggccggc aggagcggat
     2281 cgagctcgat cgcatcgggg cgctgccagg cgatggcttg gtgttcgacc gcgcgaggct
     2341 cgccgcgcca ggcgtagacc ctatagaaat gcaggcgcac tttggcgtgc ggatagacga
     2401 agacccgggt gatccaagga taagcgcgct cgacctcgat gccgagctcc tcgcggacct
     2461 cgcgcctgag cgcctcgggg acaggctcgc ctggctcgat tttgcctccg ggaaattccc
     2521 agtagcccga gtagaccttg gtttcgggac gctgagccag aagcgcggcg ccgtcggaac
     2581 ggatcagcac cgccacggcg acgtccaccg gggccgacat cgtggacacc tgctagcgtc
     2641 ttgccttcgc gtcgccgggg ttgcgcccgg cgtagtcgcg cgcgaactgc cacgccacgc
     2701 ggccgctgcg cgaaccgcgt agcagcgccc actgcagcgc ttcctgccga gccttgagga
     2761 tctccgcttc gctgcagccg agctcgcgca accaatgtcc cgcgatatcc aggtaggcgt
     2821 cctggtcgaa cgggtagaag gagacccaca gcccgaagcg ctcggaaagc gagattttct
     2881 cctccaccgt ttcggcggga tgaatctcct cgccgagatg cttggtctcg agattctcct
     2941 gcatgtactc gggcatgaga tgccgccggt tcgacgtcgc gtagatgagg agattttcgg
     3001 aagtcgcggt gatcgagccg tcgagcacga ccttgagcgc cttgtagccc gcggcctccg
     3061 agagctcgaa cgacaagtcg tcgcaaaaca ggataaaccg ctcgggccgc cccgccacct
     3121 gatcgacgat atcggggaga tcgatgagat cgtgcttttc cacttcgatg aggcgcagcc
     3181 cctgggggtg gaatttgtcg agcacggcct tgacgagcga ggactttccc gtgccgcgcg
     3241 caccggtcag gagcgcattg ttggccgtcc ggcgttcgac gaattggcgc gtgttctgct
     3301 cgatcagcgc cttctggcta tcgacgccgt ggagatccga aagtcggata cggtgcaggt
     3361 ttgcgacggg ctggataaag ccgcggcgcg acgtcttgcg ccagcggaac gcgacgctct
     3421 ggtgccagtc cacggggcgc aatgccggcg ggagcagcgt ttccacgcgc tcgagcaatg
     3481 cttcgccgcg cttgatcagg cgttcgagct cggacatagc ccgtcctagg tcctgtactg
     3541 ggcgttgatg gtaacgtaat cgtgcgaaag gtcgcaggtc cacaccatgg tggcggcaag
     3601 cccgcggttg agcactacgc gaaccgtgat ctcgcttttt gccatgacgc gcttcccgtc
     3661 ctcctcgcgg taagccggag cgcgccctcc gtctttcgac accagcacct cgtcgaggta
     3721 aagctcgacg cggccgactt cgagatcgcg cacgccggcg ttgccgatcg cggcgagcag
     3781 ccgtcccaga ttcgggtcgg atgcggaaaa cgcggtcttc accagcggcg agtgcgcgat
     3841 cgaataggcg acgttcgcgc attcggtttc ggtccggccc ctttccacgc agaccgtgat
     3901 gaacttggtc gcgccctcgc cgtcgcgcac gatcgcctgc gccaggtgcc gcgccacttc
     3961 gctgacagcc acctcgagct tcaggagatc gcggcggctg ctcgccgcgg gcccg
//
LOCUS       DASPZD010000432         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_526308, whole genome shotgun sequence.
ACCESSION   DASPZD010000432 DASPZD010000000
VERSION     DASPZD010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526308"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VEM33_08365"
     CDS             complement(<1..575)
                     /locus_tag="VEM33_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51880.1"
                     /translation="MPHSSEDLLNDQTGEEIIEIDENGVVHSKGRTPSQLKSTVLRDP
                     EGEYAVATELEADKHLLLTVAEAQSEHRWYYEQERLRSFPAFNLLRRDGVIVAAEGAI
                     DTQNNNTYGVRIDLTNFPYALPKPFPKGWPLHPDVPHKYNDGSLCIMRSAQWRTTFTV
                     AFVVAKTAIWLAKYEIWKRNGHVWPGKHQRH"
     gene            complement(608..1345)
                     /locus_tag="VEM33_08370"
     CDS             complement(608..1345)
                     /locus_tag="VEM33_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYS51881.1"
                     /translation="MIPTYTFFAVDKGNMTLIEFPNGVSMVVDCRRSETRPSPLEYLK
                     QKGLRRLDFLVITHPHRDHLSGIEEMCEAIKPKYLWHNGRFFKPDPVYDDWLYYEKLR
                     NGQLSYCESTKVRAGQTATIGNAKLYVSAPRAPHLEGTTDDENNNGIILTVTAEKSKV
                     VITGDTQHEQWDVVDLGPLSNASIFLASHHGRENGFVEEVMETLDPQHIVISDGEPGD
                     TDATDEYERFAPVSTTREGSVVVRPQD"
     gene            complement(1544..2806)
                     /gene="glyA"
                     /locus_tag="VEM33_08375"
     CDS             complement(1544..2806)
                     /gene="glyA"
                     /locus_tag="VEM33_08375"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYS51882.1"
                     /translation="MTDRLAPLSESDPKIADLIKREVARQEHGLELIASENFVSYAVL
                     EAMGTALTNKYAEGLPGKRYYGGCEVVDQVEQLAIDRAKQLFGAEHANVQPHSGAQAN
                     MAAYLAVAKFGDTLLGLALPHGGHLTHGSPVNFSGTLFKATAYTVREDTGRIDYDVVR
                     EVAKKEKPRIIIGGGSAYARIVDFPALRSIADEVGAVLVVDMAHFAGLVAGGVYPSPI
                     PHAQIVTTTTHKTLRGPRGGMILCKAEHAKAVDKQVFPGTQGGPLEHVIAAKAVALLE
                     ASHPDFKDYAKQVVANAQALANRLITLGYAIVSGGTDSHLMLVDLRAKTITGKDAEQL
                     LDRAAITVNKNTIPGDPQSPFVTSGIRLGTPAVTTRGFGVEEMERVAELIDEVLVKRD
                     EATVVRVKGEVRAMVEEFPLYAKGVGVG"
     gene            complement(2864..3301)
                     /gene="rpiB"
                     /locus_tag="VEM33_08380"
     CDS             complement(2864..3301)
                     /gene="rpiB"
                     /locus_tag="VEM33_08380"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HYS51883.1"
                     /translation="MSEKIYVGADHVGFNLKNRLVDELKKLGYEPIDVGPKKLDPADD
                     YPDYAKPVASAVSSGEVQRGVLTCGTGLGMSYTANRFPKVRAALAWNEQIAELSREHG
                     DSNLLVLPAQFVSEQQGINILHKWLETNFEGGRHQRRIEKIEK"
     gene            complement(3294..>3599)
                     /locus_tag="VEM33_08385"
     CDS             complement(3294..>3599)
                     /locus_tag="VEM33_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HYS51884.1"
                     /translation="DIIAGGANNQLAEERHGDLLEERGLTYAPDYVINAGGLVNVNAE
                     LEGWTLERARNKAGEIYDTILMVFDIAAEERIPSYKAADRLAERRIAAIAKVRQNFV"
BASE COUNT          628 a         1196 c         1014 g          761 t
ORIGIN      
        1 tagtgccgtt ggtgcttccc aggccacaca tgcccgttgc gcttccaaat ctcgtatttc
       61 gcgagccaga tcgcggtctt cgcgacgacg aacgccactg tgaaggtcgt gcgccactgg
      121 gctgagcgca tgatgcacaa ggagccgtca ttgtacttgt ggggcacgtc gggatggagc
      181 ggccagccct tcggaaaggg cttcgggagg gcgtacggaa agttcgtcaa gtcaatccgg
      241 acgccgtacg tgttgttgtt ctgcgtatca atcgcgccct cagccgcgac gatgactccg
      301 tcacggcgga gcagattgaa cgcggggaac gagcggagcc gttcttgctc gtagtaccac
      361 cggtgttctg actgcgcctc ggcgaccgtt agcagcaggt gcttgtccgc ctcgagttca
      421 gtcgccacgg catactcgcc ctcggggtcg cggagtaccg tgctcttgag ttgcgacggt
      481 gtccgcccct tcgagtggac gacaccgttc tcatcaatct cgatgatctc ctcgccggtc
      541 tggtcgttca gcaggtcttc agaactgtgc ggcatcgcct tggccctcct cgtgcacttg
      601 gtttgaccta gtcctgcggc cggacaacta ccgaaccttc ccgcgtcgtt gagactggag
      661 cgaatctctc gtattcgtcc gtggcatcag tatcgccggg ttctccatcc gagatcacga
      721 tgtgctgcgg gtccaaggtc tccatgactt cctcgacgaa tccgttttcc cgcccgtggt
      781 gactggcgag gaaaatcgat gcattcgaca ggggtccaag gtcaacgaca tcccactgct
      841 cgtgctgggt gtcgcccgtg ataacaacct ttgacttctc cgccgttacc gtgaggatga
      901 ttccgttgtt gttctcgtca tccgtcgtgc cttcaaggtg cggagcacgc ggcgcggaca
      961 catagagctt ggcattgccg atcgtggcgg tttggcccgc tcgcactttc gtggattcgc
     1021 agtacgagag ctgcccgtta cgcaacttct cgtagtagag ccaatcgtcg tagacaggat
     1081 cgggcttgaa aaagcgcccg ttgtgccaca agtacttggg cttgatcgcc tcgcacatct
     1141 cctcgatgcc gctcaggtgg tcgcgatgtg gatgcgtgat caccaggaag tcgaggcgcc
     1201 ggaggccctt ctgcttcagg tactcgagag gggacggtcg agtctccgaa cgtcggcagt
     1261 ccacgaccat tgacacgccg ttggggaact cgatcagggt catgttcccc ttgtccactg
     1321 cgaagaacgt gtacgtcggt atcatgtcac ctccccgcca cgaacaaatc ccgtaggaca
     1381 tggatagaaa aaagcccacc gcgtttggcg gtaggctctt tggtctgccg tcgagtggat
     1441 aacgggaaac taactcaatg gaccggtcaa tcaaggccat tcgatacgct ttccaaacag
     1501 cttgccgctg gggtccagca atgtggattt gttcgcggca ccctcacccc acccccaccc
     1561 ccttcgcata caacggaaac tcctccacca tcgcccgcac ctcgcccttc acccgcacca
     1621 ccgtcgcctc atcgcgcttc accagcacct catcgatcag ctcagccacc cgctccatct
     1681 cctcgactcc aaagcctcgt gtagtaacgg cgggcgtgcc gagtcggatg ccgctggtga
     1741 cgaacggcga ctgtggatcg cccggaatcg tgttcttgtt caccgtgatc gccgcgcgat
     1801 cgagcagctg ctcggcatcc ttccccgtta ttgttttcgc gcgcagatcg accagcatca
     1861 gatggctgtc cgtcccgcct gacacgatcg catagccgag cgtgatcaac cggttggcga
     1921 gcgcctgggc gttcgccacg acctgcttcg cgtaatcctt gaagtcgggg tgtgacgcct
     1981 caagcagtgc gacagccttc gccgcaatca catgctcgag cggcccgccc tgcgttccgg
     2041 ggaagacctg cttatcgacg gccttggcgt gctcggcctt gcacagaatc atgccgccgc
     2101 ggggaccgcg cagcgtcttg tgtgtggtgg tcgtgacaat ctgtgcatgg ggaatggggg
     2161 acgggtacac gccccccgcc acgagcccgg caaaatgcgc catgtccacg accagcaccg
     2221 cgccgacttc atccgcgatc gagcgcagcg ccgggaaatc gacgatgcgc gcataggcgc
     2281 tgccgccgcc gatgatgatg cgcggcttct ccttcttcgc gacctcgcgc accacatcgt
     2341 aatcgatccg ccccgtgtcc tcgcgcaccg tgtacgccgt cgccttgaac agcgtgccgg
     2401 agaaattcac cggcgaccca tgcgtgagat ggccgccatg cggcagcgcc aggccgagca
     2461 gcgtgtcgcc gaacttcgcg acggcgagat acgccgccat gttggcctgc gccccggagt
     2521 gcggctgcac attcgcatgc tcggcgccga acagctgctt cgcccggtcg atcgcgagct
     2581 gctcgacctg atccaccacc tcgcagccgc cgtagtagcg cttccccggc aacccctcgg
     2641 catacttgtt cgtgagcgcc gtccccattg cttcgagcac agcgtaggac acgaagttct
     2701 ccgaggcaat cagctccaac ccatgttctt gcctcgccac ctcacgcttg atgaggtccg
     2761 cgatcttggg atcggattcc gacagcggcg ctaggcgatc agtcatctac cgtccggttt
     2821 gtttgaggta ggggcgcagc atgctgcgcc cctacccctc gtattatttc tcgatttttt
     2881 cgatccggcg ctggtgccgg ccaccttcga aattcgtctc caaccactta tgcaaaatgt
     2941 tgatgccctg ctgttcgctc acgaactggg ccggcaggac gagcagattc gagtccccgt
     3001 gttctcggga tagctcagca atctgctcat tccatgccag tgccgcccgt accttcggaa
     3061 accggttcgc agtatacgac atgcccaatc ctgtgccaca ggtgaggacg ccgcgctgca
     3121 cttcgccgct gctcaccgcg ctcgcgacgg gcttggcata atcgggatag tcatctgccg
     3181 ggtcgagctt cttcggcccc acgtcgattg gctcataccc gagcttcttc aactcgtcca
     3241 ccaaccgatt cttcaagttg aacccgacgt gatccgcccc gacatagatc ttctcagaca
     3301 aagttctgcc tcaccttcgc gatcgcggca atgcgccgct ccgccagccg gtccgccgcc
     3361 ttatagctcg gaatccgctc ctcagccgcg atgtcgaaca ccatcagaat cgtgtcgtaa
     3421 atctcgcccg ccttgttgcg cgcccgctcc agcgtccacc cctcgagctc ggcgttgacg
     3481 ttgaccaacc cgccggcgtt gatcacgtag tcgggcgcat acgtgagacc gcgctcctcg
     3541 agcagatcgc catggcgctc ttcggccagc tgattgttcg ccccgccggc gatgatgtc
//
LOCUS       DASPZD010000433         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1643864, whole genome shotgun sequence.
ACCESSION   DASPZD010000433 DASPZD010000000
VERSION     DASPZD010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643864"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VEM33_08390"
     CDS             complement(<1..888)
                     /locus_tag="VEM33_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HYS51885.1"
                     /translation="MNKGLIDVDEALERLLTGTRPVAETETVATLSATGRVLATAQRS
                     TMNVPPMDNSAMDGYAVRLADLKGPEPRLKVSQKIPAGSTGKALAPGTAERIFTGAPI
                     PAGADAIVMQEHCVAEGDVVVVKKIPERGEWIRLTGSDIRTGGEVLPAGIRLRPQDTG
                     LAASVGIASLPVYRKVRLGLFFTGDELVMPGDPLPPGRIYNSNRFTLNGLAGIFGCAT
                     RDYGIVPDSLDATRAVLRRAASECDVIVTSGGVSVGDADFVKPAVEAEGELLMWKIAM
                     KPGRPLAFGRVGKANFIGLP"
     gene            complement(885..1379)
                     /gene="mobB"
                     /locus_tag="VEM33_08395"
     CDS             complement(885..1379)
                     /gene="mobB"
                     /locus_tag="VEM33_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HYS51886.1"
                     /translation="MKIFGFAGWSGSGKTTLIEQLVPRFVKRGLRVSMIKHAHHSFDV
                     DQPGKDSYRHRQAGCSEVMVVSDRRWVIMHELRGEPEPSLEEQIKRVSPCDLLLVEGH
                     KHHPLPKLEVWRRENGKPLLYPEDGHIVAIASDTALETKLPRFDLDDHDGITDFILSY
                     NGFR"
     gene            complement(1433..>2096)
                     /locus_tag="VEM33_08400"
     CDS             complement(1433..>2096)
                     /locus_tag="VEM33_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYS51887.1"
                     /translation="TGIQVRVVALGTGQALDLARRGDADVVFVHARSAEEKFLAEGHG
                     VRRFDVMYNDFVLVGPKADPAKVSGGKDILEGLKKIKAASAPFVSRGDKSGTHIAELD
                     LWKAAGIDIAVGKGPWYRDTGQGMGPALNTAAAMNAYILADRGTWISFKNRGELAIAV
                     EGDKRLFNQYGVMLVNPAKHPNVKNDLGQAFVDWVISPEGQKTIAQYKIGGEQLFFPN
                     AK"
BASE COUNT          362 a          689 c          651 g          394 t
ORIGIN      
        1 gggaagcccg atgaaattgg ccttgccgac acgtccgaac gcaagcgggc ggcccggttt
       61 catagcgatc ttccacatga ggagctcgcc ctcggcttcg acggcgggct tgacgaagtc
      121 cgcatccccc acggagacac cgccgctggt gacgatcacg tcgcattcgc ttgcggcgcg
      181 ccgcaacacc gcccgcgttg catcgagtga atcgggaacg atcccgtagt cgcgggtcgc
      241 gcatccgaaa ataccggcga ggccattgag ggtgaaacga ttcgagttgt aaatccgccc
      301 cggcggcagc gggtcacccg gcatgacgag ctcgtcgccc gtgaaaaaca agcccagacg
      361 caccttccgg taaacgggga gcgatgcgat gccgaccgaa gccgcgagcc cggtgtcctg
      421 cggccgcaac cggatccccg cgggaaggac ctccccgccc gtgcgaatat cgctgccggt
      481 gaggcggatc cattctccgc gctcagggat ttttttgacg acgaccacgt cgccttcggc
      541 aacgcagtgt tcctgcatca cgatggcgtc ggcgccggct ggaatcggcg cacccgtgaa
      601 aatgcgctcg gcggtccccg gagcgagcgc cttgcccgtc gagcctgcgg ggattttctg
      661 ggagaccttg agccggggtt ccggtccttt cagatcggcg agccgcaccg cgtaaccgtc
      721 catggcgctg ttgtccatcg gcggcacgtt catcgtcgag cgctgagcag tggcgagcac
      781 ccggccggtc gcggacaagg tcgctaccgt ttcggtctcg gcaacagggc gcgtgccggt
      841 gaggaggcgc tcgagcgcct cgtccacgtc gataagtccc ttgttcatcg gaatccgttg
      901 tagctcagga tgaaatctgt gatgccgtcg tgatcgtcga gatcgaacct cggcaacttg
      961 gtttcgagcg cggtgtcgga ggcgatggcc actatatgtc catcctcagg atacagcagc
     1021 ggcttgccgt tttccctgcg ccagacttcg agcttcggca ggggatgatg cttgtggcct
     1081 tcaacgagca gcaggtcgca gggcgagacg cgcttgatct gctcctccag agaaggctcc
     1141 ggctcgccgc gcagctcgtg catgatcacc cagcgccggt cggacacgac catgacctcg
     1201 ctgcagcccg cctggcggtg gcggtaagaa tccttgcccg gttggtcgac gtcgaaactg
     1261 tgatgcgcgt gcttgatcat cgataccctg agaccgcgct tgacgaagcg cgggacgagc
     1321 tgctcgatca aggtggtctt gccgcttccc gaccatccgg cgaagccgaa gatcttcatg
     1381 cacgccattg tatcagggcg tcattcccgc acggatatag cgagcgggcc gctcacttcg
     1441 cgttcggaaa gaacagctgt tccccgccga tcttgtactg ggcgatcgtc ttctgtccct
     1501 cgggcgagat gacccagtcg acgaacgcct ggccgagatc gtttttcacg ttgggatgct
     1561 tggcaggatt caccagcatc accccgtact ggttgaacaa gcgcttgtcg ccttcaaccg
     1621 cgatggcgag ctcgccccgg ttcttgaacg agatccaggt gccgcggtcg gcgagaatgt
     1681 aggcgttcat cgccgcagcg gtgttgagtg ccgggcccat tccctgaccc gtatcgcgat
     1741 accacggccc cttccccaca gcgatgtcga tccccgcagc tttccataga tcgagctcgg
     1801 cgatatgcgt gccgctcttg tcgccgcggg agacgaacgg cgcgctcgcc gccttgattt
     1861 tcttcaatcc ctccaggatg tccttgccgc cggagacttt cgccggatcc gccttcggcc
     1921 cgaccaacac aaaatcgttg tacatgacgt cgaagcgccg aacgccgtgg ccctcggcga
     1981 ggaatttctc ctcggccgat cgcgcgtgca cgaacaccac gtcggcatcg ccgcggcgcg
     2041 cgagatcgag cgcctgcccc gtgccgagcg cgaccacgcg cacctggatc ccggtc
//
LOCUS       DASPZD010000434         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1611672, whole genome shotgun sequence.
ACCESSION   DASPZD010000434 DASPZD010000000
VERSION     DASPZD010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611672"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..>2047
                     /locus_tag="VEM33_08405"
     CDS             531..>2047
                     /locus_tag="VEM33_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYS51888.1"
                     /translation="MRAPRILLIGPYDPLCGEYTFLAPPLGVWRLAGVLESAGVEVKV
                     FDPNCCAGPPGRALEREILGCPWDVIGVSTTGMTLRFDLELAHLARRLAPRALLVAGG
                     MEATFKPELMFRLGPFDCVVLGEGERPLLELVARLRSGESLRGVTGTAERAADGGCLR
                     MPQQALARTELRDAIFRTPYEKMPYSAYWERLEEAYRVGALPTKAAREARLAEIRSVR
                     LITLNYCPMGCTFCSSTNFLHEAQGSVASIARLDAEECVQMIQRIVSTHPRVRTVIFQ
                     DDIFVFTKDRRILPLCEAIIAAKESGTVPRELQFISTNRIDAMSPERLAAMRRAGFRV
                     LGFGIENFSRNVLGEFNKAHIYSHIQPTLTSALELGITPFLDLILSSPRATLADVATT
                     LREAYHWLRQGCEVGMYPYVIPFSGAAMSADPELRAVTVYERRQVAGTEIEWNQPSKI
                     LPMDPAVRETILRIERDFEESLLQLQPRAAHLPSRLRSLLWIQCSLQAMAEQALP"
BASE COUNT          340 a          633 c          675 g          399 t
ORIGIN      
        1 cgcctgctcc acagccggct gcaggccatc gaaagtgtgt gccgcgctga ggtgtactca
       61 aggacaggtg ttgcaagcgc tgacgcgtag cccgcgtcgc catacaccac cgacatgctg
      121 gcggttcgcg aacgccagct gctgcacctg atcggcgggc catcgggcgt ccgggcgaac
      181 gccgtcgagt catgccgctc cgaatccacc tgaaagagtg tggtcagcca caggtgtagt
      241 gtgtagtgcc gtatcgggaa attcctgatg cggtgttgcg tctttggccg acacgctctt
      301 gcggctttcc ccggtttgac gcgcagcccg ggggcactac gctggctagg ttctctggat
      361 gggagacgct tcctaatagt agtttgccga ggtttgcgcg cgcggctcga gccggaagcc
      421 gatgatgccg cggcagccac gcggttcgcg gggggtaaag gtctggctgc gaatgcgcat
      481 acggcaccga gcgctcgccg tcaggccaca caaggaggcc agctcaattg atgcgcgcgc
      541 cccggatcct gttgattggg ccttacgacc cgctgtgcgg ggagtacacc tttctcgctc
      601 ctccgctggg agtgtggcgc ctggcgggcg tgctcgaatc cgccggggtc gaggtcaagg
      661 tcttcgatcc gaactgttgt gcagggccac ccggtcgcgc cctcgagcgc gagatcttgg
      721 ggtgtccatg ggacgtcatc ggcgtctcga ccacgggaat gacgcttcgc ttcgatctcg
      781 agctggcgca cctcgcacgg cgtttggcgc cccgggcact gctggtcgcg ggtggtatgg
      841 aggcgacctt caaaccggag ctcatgttcc gtctcgggcc ctttgattgc gtcgtgctgg
      901 gggagggcga gcgcccgttg ctcgagttgg tcgcgcgact gcgttccggg gagtccttgc
      961 gcggggtcac cggaacggcg gagcgcgccg ctgacggggg gtgcttgaga atgccgcagc
     1021 aggcgctcgc tcgcacggag ctgcgagacg cgatcttccg cacgccctat gaaaagatgc
     1081 cttacagcgc ctactgggag cggctcgaag aggcgtaccg cgtcggcgcc ctgccgacga
     1141 aggcggctcg cgaggcgcga cttgccgaga tccgttcggt gcgcctgatc accctcaact
     1201 actgccccat gggttgcacc ttctgctcgt cgacgaactt cctgcacgag gcccagggaa
     1261 gtgtcgcgag tatcgcgcgg ctcgatgcag aagaatgtgt acagatgatt cagcgtattg
     1321 tgagtaccca cccccgggtg cgcaccgtca tctttcagga tgatatcttc gtcttcacaa
     1381 aggatcgacg cattctgccg ctatgtgagg cgatcatcgc cgccaaggag agcggcactg
     1441 tgccgcgtga actgcagttc atcagcacca atcgtatcga cgccatgtcg cccgagcggt
     1501 tggcagccat gcggcgggcc ggattccggg tgctcggttt cgggatcgag aacttttcgc
     1561 gcaacgtact cggcgaattc aacaaagccc atatctacag tcatatccaa ccgacactga
     1621 catcggctct ggagctgggc atcacgccgt tcctggatct gatcctgagc tccccacggg
     1681 cgacgctcgc agacgttgcc acgaccttgc gcgaagccta tcactggttg cggcaggggt
     1741 gcgaagtcgg aatgtatccc tacgtgattc cgttctcggg agccgccatg tcggcagatc
     1801 ccgagctgcg agcggttacc gtctacgagc gccggcaggt cgccgggacg gagatcgagt
     1861 ggaatcaacc cagcaagatt ctgccgatgg acccggcggt acgcgaaacc atcctgcgca
     1921 tcgagcgcga cttcgaggag tcgttgctgc agcttcagcc gcgcgccgcg catttgccgt
     1981 cccggctgcg ctcgctgctg tggattcagt gctctttgca ggccatggcc gagcaggcac
     2041 tgccgat
//
LOCUS       DASPZD010000435         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_45888, whole genome shotgun sequence.
ACCESSION   DASPZD010000435 DASPZD010000000
VERSION     DASPZD010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45888"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VEM33_08410"
     CDS             complement(<1..1574)
                     /locus_tag="VEM33_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYS51889.1"
                     /translation="MNARIDLSLSRREFLKASGALVVSAAGPAFIGEALAQAAAGSAG
                     GKPPLVPTELDSWVAILPDGRVTAFFGKMDMGQSLDIAIAQIVADELDVDVAKVDVIM
                     GDTALTCNQGGASGSTGIQTGARPLRSAAAEARRLLMQSASQKVGLPVEQLRCDDGVV
                     SAVSDPAKKASYAELIGGRHFHHQVEWNKEIGNPMDIKVQAKPKSPSEYRVVGKSLPR
                     RDVAGKVYRTEKYVTDVSLPKMLHARVIRCSRAACTVGGVDEGSISAVKGARVIREKD
                     FVAVVADHEWDAVRASRMLKISWNEMKKDPFPPMAEIYDHIRKAPVVKREDPVKKGDV
                     EGVFKTAARVVEAEYEWPFQSHASMGPACAVADVRSDGATVWTGSQKPHFVQTGVARL
                     LGVPPEKVHAIWVTGPGSYGRNDAGDAALDAAFLSKLTGRPVRVQGMRNDGIAWDPKG
                     PASVHRGRAALDASGKVIAYEFISKGFSRRNIDTNESDPRDSLAGMAIGLPPKPSLDF
                     GVPAEAYGFDNKLLAW"
     gene            complement(1584..2048)
                     /locus_tag="VEM33_08415"
     CDS             complement(1584..2048)
                     /locus_tag="VEM33_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYS51890.1"
                     /translation="MASIKFAVDGRSVTVNAVPSMPLLYALRNDLRMSNPHFGCGLAQ
                     CGACTVHLDGEPVRSCVMPLSAVAGKKVTTLQGLGTPEKPHPLQAAYVEEQVPQCGYC
                     INGWIMTAAAFLKKKPKASDAEIRDALAGLKCRCATHAAIMRAIKRAQTQMA"
BASE COUNT          373 a          775 c          789 g          418 t
ORIGIN      
        1 tcccaggcga gcagcttgtt gtcgaagccg taggcctcgg cgggcacgcc gaagtcgagg
       61 ctcggtttgg gcggcagccc gatagccatg ccggcaaggc tgtcgcgcgg atccgattcg
      121 ttcgtatcga tattcctgcg cgagaagccc ttggaaatga actcgtacgc gatcactttt
      181 cccgaagcat cgagcgcggc gcggccgcgg tgcaccgacg ccgggccttt cgggtcccag
      241 gcgatgccgt cgttcctcat gccctgcacg cgcaccgggc gccccgtgag cttggagagg
      301 aacgccgcat cgagcgcagc gtcgccggcg tcgttgcgcc cgtaggagcc ggggccggtg
      361 acccagattg cgtgcacctt ctcgggcgga acaccgagga gccgcgccac cccggtctgg
      421 acgaagtgcg gtttctgcga gccggtccag acggtagcgc catccgagcg cacgtcggct
      481 accgcgcagg cgggccccat gctcgcgtgc gactggaacg gccactcgta ctcggcttcg
      541 acgacgcgag cggcggtctt gaacacgccc tcaacgtcgc ccttcttcac cgggtcttcg
      601 cgcttcacca cgggcgcctt gcggatgtgg tcgtagatct ccgccatcgg cgggaaaggg
      661 tccttcttca tctcgttcca cgagatcttg agcatgcgcg aagcacgcac cgcatcccac
      721 tcgtgatcgg ccactaccgc gacgaaatcc ttttcacgga tcacccgcgc gcccttgacc
      781 gcactgatcg agccctcatc gactccgcct acggtgcagg cggcgcgcga gcaccggatg
      841 acccgggcgt gaagcatctt cggtagagag acatcggtca cgtacttctc ggtacggtag
      901 accttccccg caacgtcgcg gcgcgggagc gatttcccga ccacgcggta ttccgaaggc
      961 gacttcggct ttgcctggac cttgatgtcc atgggattgc cgatctcctt gttccactcc
     1021 acctggtggt ggaagtggcg gccgccgatc agctcggcat agctcgcctt cttcgcggga
     1081 tcggaaacgg cgctgaccac gccgtcgtcg caacgcagct gctcgaccgg gagcccgact
     1141 ttctgcgaag cggactgcat cagcagccgg cgcgcttcgg ccgccgcgct gcgcaagggg
     1201 cgggcgccgg tctggattcc ggtgctgccg gaggctcctc cctggttgca ggtgagcgcg
     1261 gtgtcgccca tgatgacgtc gaccttggcg acgtcgacgt cgagctcgtc ggcgacgatc
     1321 tgcgcaatcg cgatgtccag ggactggccc atgtccatct tgccgaagaa ggcggtgacg
     1381 cggccgtcgg gcaggatcgc cacccaggaa tcgagctcgg tcgggacaag cggcggtttg
     1441 ccgccggctg aacccgcggc ggcttgcgcg agcgcctcgc cgatgaacgc cggtccggca
     1501 gcggacacga cgagcgctcc ggacgccttc aggaattcgc ggcgggaaag tgacagatcg
     1561 atgcgtgcgt tcatgtcatt ctcctaggcc atttgtgtct gggcacgctt gatcgcgcgc
     1621 atgatcgccg catgcgtcgc gcagcggcac ttgagcccgg caagcgcatc ccggatctcg
     1681 gcgtcgctcg ccttcggctt ctttttcagg aacgccgccg cggtcatgat ccagccgttg
     1741 atgcagtagc cgcactgcgg cacctgctct tcgacgtatg cggcttgcag cggatgcggc
     1801 ttttccggcg tgcccaggcc ctggagcgtg gtcaccttct ttcctgccac ggccgagagc
     1861 ggcatcacgc aggagcgcac cggctcgccg tccagatgca cggtgcacgc gccgcactgc
     1921 gcgagtccgc agccgaagtg cggattgctc atcctgagat cgttgcgcag cgcgtagagg
     1981 agcggcatgc tcgggacggc gttgaccgtc accgatcttc cgtcgacggc gaatttgatg
     2041 cttgccatgg agcctccttt ggatgtacgg ccgatggatc cgggccggta gatgtcgcga
     2101 aactagtgta gacccggggc gatgcctgcg caacgcaata gccgccctcg ccgcggtggt
     2161 acgatacgcc gttgatttcg gcgaggagga gtcgcagcgg tgaaaacggg cgcccgcgaa
     2221 accgggatcg acctcgagcg gttcaggctg cgccgctttg tagaggaatt gcgacagacg
     2281 ggcgagctcg aagtgaaggt gggagccgcg gagctcgcgg aggtcgcccc ggcgctcgag
     2341 ggcaacgcca gggca
//
LOCUS       DASPZD010000436         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_658932, whole genome shotgun sequence.
ACCESSION   DASPZD010000436 DASPZD010000000
VERSION     DASPZD010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658932"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..747
                     /locus_tag="VEM33_08420"
     CDS             4..747
                     /locus_tag="VEM33_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51891.1"
                     /translation="MLEVRGLRKSFGALLASDDVDLEVLEGETHAIIGPNGAGKTTLI
                     SQLAGSLLPDRGRVRFAGEDITALSAPARARRGFARSFQITSIYPDFTALENVMLAVQ
                     AHSGSSFRFWRPAREEASLRSPASAILEEVGLSGRGDVLAANLAHGEQRALEIATALA
                     TRPRLLLLDEPVAGMGAEETQRMIALLSSLKGGKTLILVEHDMDAVFSLADRISVLVY
                     GRIIATGRPEEIRANSEVRRAYLGEEIAA"
     gene            747..1448
                     /locus_tag="VEM33_08425"
     CDS             747..1448
                     /locus_tag="VEM33_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51892.1"
                     /translation="MLEVDALVTAYGQSQVLFGVSFGVRPGEAVTLLGRNGMGKTTTV
                     RSVMGMVRPRGGTIVFEGKPLHGLPSYRVAQAGLGLVPEGRQVFPNLTVRENLVATAA
                     KRSGRTEPWTLARVFALFPQLAERRTHFGSQLSGGEQQMLAIGRALMTNPRMLILDEA
                     TEGLAPLVRIEIYRAIEGLKASGLAILIIDKDLRALTRIADRHYVLEKGRVVWSGASA
                     ELVANRELQHRYLGV"
     gene            1511..2248
                     /locus_tag="VEM33_08430"
     CDS             1511..2248
                     /locus_tag="VEM33_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51893.1"
                     /translation="MLSIRNLCKSFSGPRPKTVLRDISFELAAGDYVVVMGESGIGKS
                     TLLNLIAGLERPDSGQVLLDGADLCALGDDALTLVRRKHMGFVFQAFHVLPYLTVAQN
                     VALPLALNGIGKRPMRGRVGALLESVRLLERGESLPHELSGGETQRVAIARALVHKPR
                     LVLADEPTGNLDPESAKEVLALLRECVKGEGAAGILVTHSAAAARTADRAYMLTQGGL
                     RALGHAGASGQADRRRGERDRRRAPRS"
     gene            2304..2624
                     /locus_tag="VEM33_08435"
     CDS             2304..2624
                     /locus_tag="VEM33_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51894.1"
                     /translation="MLTTIILAGGLSACATTKTEDVKVYTPTQLKQGEYETVKRLWVE
                     TWRTPFWVPNYSSPKDGIAGLQDKAAALGANGLINVDCYGDKGLFSFGSESFVCYGKA
                     IKVP"
     gene            complement(2650..3153)
                     /locus_tag="VEM33_08440"
     CDS             complement(2650..3153)
                     /locus_tag="VEM33_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYS51895.1"
                     /translation="MSDHAATLAAALIRNFNEAPLTRLLREPQYDAQGARLAKGTAAR
                     KLGASIDILAPGKRGCPYHLHHAQEEMFIILEGNGTLRVAGEMLPLRAGDVIFIPPGP
                     EYPHQIINTSAAPLKYLSISTMETPEICEYPDSGKFLAEGSLDKATPFDVIGRRGPSL
                     DYWEGEP"
     gene            complement(3229..4410)
                     /locus_tag="VEM33_08445"
     CDS             complement(3229..4410)
                     /locus_tag="VEM33_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYS51896.1"
                     /translation="MPAPLNLDARLAAIHAAEYPRFSDAEMARRRAALARVMEETGVD
                     HLLMCGEQRAGTGVAWLTSWPTTVEAYAIVAPKEPQVMYVEWYNHWPLARKLARDTEV
                     RWGEHRGIDKAIADLKARGAKRVGLMGALGLSKCRKLEAHFGELVDLNREYVRLRLVK
                     SREEIDWMRIAAALTDLAIEGLRREARPGMTEHELGAICEAAYHPQGGVTYIHYFLVT
                     PMASPEYCVPRQFTSTRKIRPGDVIATEITAQFFDYPGQILRTFTVAAEPTPLFRKLY
                     DTAEAAFDAVTRVIRHGTRMQEIIDAARVIEDAGFTVYDDLMHGFGGGYFPPVLGSKS
                     RPAGPLPEMTLEAGMTCVVQPNVITPDQKAGVQVGELVLVTETGFERLHRAERGLVRI
                     G"
     gene            4632..5075
                     /locus_tag="VEM33_08450"
     CDS             4632..5075
                     /locus_tag="VEM33_08450"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYS51897.1"
                     /translation="MKWNSKGRGAYAVVSAGALVLGLAACDQIQSLWKSDEKTNPVAG
                     SVGQPKPAEGTIQVARSVDLPKSIEAPKPPVDENKLLTNKIKAALGADPALKVLAIDA
                     SVSDGAVTLYGTANNRALREKAAKVVSGVPGVKSVKNELVIVAGS"
BASE COUNT         1016 a         1853 c         1979 g         1052 t
ORIGIN      
        1 accctgctcg aagtccgcgg cttgaggaag agcttcgggg cgctcctcgc gagcgacgac
       61 gtcgacctcg aggtcctcga aggcgagacc cacgccatca tcgggccgaa cggcgccggc
      121 aagacgaccc tgatcagcca gctcgccggc agtctgcttc ccgaccgggg ccgcgtacgc
      181 ttcgccggcg aggacatcac ggcgctctcg gcgccggcgc gggcacgccg cggattcgcg
      241 cgctcattcc agatcaccag tatctatccc gatttcaccg cgctcgagaa cgtgatgctc
      301 gcggtgcagg cgcactccgg ctcgagcttc cggttctggc ggcctgcgcg cgaggaggcg
      361 agcctgcgat cgcccgcaag cgcgattctc gaggaagtgg gcctgagcgg gcgcggtgac
      421 gttctcgcgg cgaacctcgc gcacggcgag cagcgcgcgc tcgagatcgc gaccgccctc
      481 gcgacgcgcc cgcggctcct gcttctcgac gaaccggtcg cgggaatggg cgcggaggag
      541 actcagcgca tgattgccct gctttcatcg ctaaaagggg gaaagaccct tatcctcgtc
      601 gagcacgaca tggatgcggt attttccctc gccgaccgca tctcggtgct ggtctacggg
      661 cgcattatcg ccacgggaag gcctgaagag atccgcgcga attccgaagt gcggcgcgct
      721 tatctcggcg aggagatcgc ggcgtgatgc tcgaggtcga tgcgctggtc accgcctatg
      781 ggcagagcca ggtgctgttc ggcgtctcgt tcggcgtgcg cccgggcgaa gcggtcacct
      841 tgctcggccg caacggcatg ggcaagacca ccacggtgcg atcggtcatg ggcatggtgc
      901 gcccgcgcgg aggcacgatc gtgttcgaag gcaagccgct gcacggcctg ccttcgtacc
      961 gcgtcgcgca ggcggggctg ggcctcgtgc cggaagggcg gcaggtgttt cccaacctca
     1021 cggtgcgcga gaacctcgtg gcgactgccg ccaagcgctc cgggcgcacc gagccctgga
     1081 cgctggcgcg cgtgttcgcg ctcttcccgc agctcgccga aaggcgcaca cacttcggca
     1141 gccagctctc cggaggtgag cagcagatgc tcgccatcgg gcgagccttg atgaccaatc
     1201 cgcgcatgct catcctcgac gaagccaccg aaggcctcgc gccgctcgtc cgcatcgaga
     1261 tctaccgggc gatcgagggc ctgaaggcct cgggcctggc catcctgatc atcgacaagg
     1321 acctgcgcgc cctgacgcgc atcgccgacc ggcactacgt gctcgagaaa ggccgggtcg
     1381 tctggagcgg cgcctccgcc gagctggtgg cgaaccgcga gctgcagcac cgctatctgg
     1441 gcgtgtgacg ggtccgtgga cccgcgcggc gcgcttgcct gcaagcccct gcgcaagtaa
     1501 cctcgcgcca gtgttgtcga tcaggaacct ctgcaagtcc ttttccggcc cgcgcccgaa
     1561 gaccgtcctg cgggacatca gcttcgagct cgccgcgggc gattacgtcg ttgtgatggg
     1621 cgaatcggga atcgggaaat cgacgctgct caatctcatc gccgggctgg agcggcctga
     1681 ttccgggcag gtgctcctgg acggcgcgga cctgtgcgcg ctcggcgacg acgccctcac
     1741 cctcgtgcgc cgcaagcaca tgggtttcgt gttccaggcg tttcacgtct tgccttacct
     1801 gaccgtggcg cagaacgtcg cgctgccgct cgcgctgaac ggcatcggga agaggcccat
     1861 gcgcgggcgg gtcggcgcgc tgctcgagtc ggtgaggctc ctcgagcgag gcgagagctt
     1921 gccgcatgag ctctcgggcg gcgagacgca gcgcgtcgcc atcgcgcgcg ccctggtgca
     1981 caagccgcgc ctggtgctcg ccgacgaacc gaccgggaat ctcgacccgg aaagcgcgaa
     2041 ggaagtgctc gcgctgctgc gcgaatgcgt gaagggcgaa ggcgcggcag gaatactcgt
     2101 cactcactcg gcggcggcgg cgaggacggc ggatagggcg tatatgctga cgcaaggagg
     2161 gctgcgagcc ctcgggcatg ccggcgccag cgggcaggcg gatcggcgac gcggcgaaag
     2221 ggaccgccgg cgagccccgc ggtcgtgaga acatagtcgc tggtcggatc gacgggctga
     2281 aagctatgaa caagcggatt gcaatgctga ctacgatcat cttggccggc ggtctttccg
     2341 cgtgcgcgac gaccaagacc gaggacgtca aggtctacac gccgacgcag ctcaagcagg
     2401 gtgagtacga aaccgtcaag cgcctatggg tcgagacctg gcgcaccccg ttctgggttc
     2461 ccaactactc ctcgccgaag gacggcatcg cgggcttgca ggacaaggcg gcggcgctcg
     2521 gtgcgaacgg actgatcaac gtcgactgct acggcgacaa gggcctcttt tccttcggct
     2581 cggagtcctt cgtctgctac gggaaggcga tcaaggttcc gtgaggcatt gttctttacg
     2641 gccccctctt cagggctcgc cctcccagta gtcgaggctt ggtccacgac gaccaatcac
     2701 gtcgaacggc gtcgccttgt cgaggctgcc ttccgcaagg aacttgcccg agtccgggta
     2761 ctcgcagatc tcgggtgtct ccatcgtgct tatggacaga tacttcaggg gcgcggcgga
     2821 tgtgttgatg atctggtgcg gatactcggg tccgggtgga ataaagatga cgtcgcccgc
     2881 ccgcagcgga agcatttcgc cggccacgcg aagtgtcccg ttgccctcga ggatgatgaa
     2941 catctcttcc tgggcatggt ggagatggta cggacaacct cgcttgcccg gagcaagtat
     3001 atcgatcgac gcgccgagtt ttcgcgccgc ggtgcctttc gccaggcgtg cgccctgggc
     3061 gtcgtattgc ggctcgcgca gcagacgagt cagcggggct tcgttgaagt ttcggatgag
     3121 agcggcggca agggtggcgg cgtgatcgga catggcggtg aggttggtgt tggttggatt
     3181 gatgacgcct tagaaatcct aaaggcggat tgtaatccgc ctcttacctt acccgatcct
     3241 gaccagcccc cgctcggccc gatgcaggcg ctcgaagccc gtttcggtga cgagcacgag
     3301 ctcgccgacc tgcactccgg ctttctgatc gggcgtgatc acgttcggtt gcacgacgca
     3361 ggtcattccc gcctccagcg tcatctccgg aagaggtccc gccgggcggc ttttcgatcc
     3421 gagcacgggc gggaagtagc cgccgccgaa gccgtgcatg aggtcgtcgt atacggtgaa
     3481 gcccgcgtcc tcgatgacgc gcgcggcgtc gatgatttcc tgcatccggg tgccgtggcg
     3541 gatgacgcgg gtcacggcgt cgaacgccgc ttccgccgta tcgtagagtt ttcggaagag
     3601 gggcgtcggt tcggcagcga ccgtgaaggt ccggaggatc tgccccgggt agtcgaagaa
     3661 ctgggcggtg atctcggtgg cgatcacgtc gcccggcctt atcttgcggg tcgaggtgaa
     3721 ttggcgcggc acgcagtatt ccgggctcgc catcggggtg acgaggaagt agtggatata
     3781 ggtcacgccg ccctgcgggt gatacgccgc ctcgcagatc gcgcccagct cgtgctcggt
     3841 catgccgggc ctcgcttcgc gccgcagtcc ctcgatcgcg agatcggtga gcgcggccgc
     3901 gatccgcatc cagtcgatct cctcgcgcga cttcaccagc cgcaggcgca cgtactcgcg
     3961 gttcagatcc acgagctcac cgaaatgggc ctcgagcttc ctgcacttcg agaggccgag
     4021 ggcgcccatc aggccgacgc gcttcgcgcc gcgcgccttg aggtcggcga tcgccttgtc
     4081 gatgccccgg tgctcgcccc agcgcacctc ggtgtcgcgc gcgagcttgc gcgcgagcgg
     4141 ccagtggttg taccactcga cgtacatcac ctgcggctct ttgggcgcga cgatcgcgta
     4201 ggcctcgacc gtcgtcggcc acgaggtgag ccaggcgacg cccgtgccgg cgcgctgctc
     4261 gccgcacatg agcagatggt cgactcccgt ctcctccatg acgcgcgcga gcgccgcgcg
     4321 gcgccgggcc atctccgcgt cggaaaagcg cggatactcc gccgcgtgga tcgcggcgag
     4381 cctcgcgtcg agattcagcg gagccggcat tgcgggagtg cgggcggggg ttcttttttc
     4441 agcgggcctt ggcggcgcaa gtgtaacctg gacggactgc aaacggttcc ggaggcgcgt
     4501 cttgacgcct gtgggatgcg cctgtcgggc agcagcgaca gtacaaaaag acgcttgcga
     4561 aaattcgatt cattcgggaa actaatcgga atccgcctgt gtctgaggtg gatatctagg
     4621 aggagcgaat catgaaatgg aacagtaaag gacgcggcgc ctatgccgtc gtaagcgcgg
     4681 gcgcgctcgt gctgggtctc gcagcttgcg atcaaatcca gtcgctgtgg aagagcgacg
     4741 agaaaaccaa ccccgtcgcc ggttcggtgg gtcaacccaa gcccgcagaa ggaacgatcc
     4801 aggtggcgag atccgtcgac ctccccaaat ccatcgaggc tcccaaacct cccgtcgacg
     4861 aaaacaagct gctcacgaac aagatcaagg ccgcactggg cgccgacccg gccctgaaag
     4921 tcctcgcgat cgacgccagc gtgtcggacg gtgcggtgac gctctacggg accgccaaca
     4981 accgggcgct gcgcgaaaag gctgcgaagg ttgtgtcggg cgttccggga gtgaagtctg
     5041 tgaagaatga gttggtgatc gttgcggggt cgtgatgcgg taggttgcgc ggtggtgtta
     5101 gtcctgcctg catccgggag cacggatcgc ggcaatgcct cacggggctt cggccccggt
     5161 tttatttctc gggtgcgaaa ggcccagacg tggtagggcg ttcttgattc ctggtccact
     5221 ggctagtcac tttgctacgt tcgcgaggcc cggtacccct gattcttcag gcgcttaggg
     5281 ctcgaccgta ggcctccaac ctcctggatg ccggcggttt cttgtagaca accgtctaca
     5341 aactgtcgtg tttacccgga cactatttgt cggacttgcc tgacagcaat tcccggtatc
     5401 tactgattag attccccatc gggcgcgacg caaagtcggc ccgcgcctcg tacctcgtgc
     5461 gagtcggaaa cgcggccgac cacccgccat cacagaggat gtgatcgcgc gcccagccac
     5521 ggcttcaagt gccgtggcac tggggagatt cgtgaccgct gaatcgtcgc aatcgtcgaa
     5581 caagcttccc ttccgggttt ccagaggcat tctttcggcg aatcgtcgcc gcgagaggag
     5641 gtcatccgcc ctttcaaact aattactgaa gaccagtctt gaacgtaaac aaggttgcgg
     5701 tagtggatcg aagcggatcg atcgcttcgg gtgtgtgcca tgtgtttcac aaacttaaac
     5761 agagggaaca atgaaaacga aattgacgaa ccgacccgtt gagcaggtga tcgccgcgat
     5821 aaacgcgctt gatctcgaat ccgtgaaggt cagggcgatg gaccccgagt tgggggaagg
     5881 ctggacgcgc gaatacgcag
//
LOCUS       DASPZD010000437         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_528759, whole genome shotgun sequence.
ACCESSION   DASPZD010000437 DASPZD010000000
VERSION     DASPZD010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528759"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VEM33_08455"
     CDS             <1..305
                     /locus_tag="VEM33_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HYS51898.1"
                     /translation="SEDAPIESRHSWDCLNLFSSCFLFNLPRHAHHHLRAGDPFWELK
                     ADPGALALESGYMAAILLAMVPPVWTRMMAGALRYWDEKLATREERALIRAAGAAR"
     gene            complement(311..679)
                     /locus_tag="VEM33_08460"
     CDS             complement(311..679)
                     /locus_tag="VEM33_08460"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HYS51899.1"
                     /translation="MRRITVVVGLLVAFASFSIDAAAARQGKRQLWGAIAYNSKSGSY
                     GYAVDRKTKREAETDAFRQCGSNCELIRTFRDACAAVAAKPTRTSSDTGASRQIAEMK
                     ALKKCGGDCAVKVWACTSEK"
     gene            complement(688..1863)
                     /locus_tag="VEM33_08465"
     CDS             complement(688..1863)
                     /locus_tag="VEM33_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYS51900.1"
                     /translation="MLSGFFVKLKDHKIPVSIKEWLTLLEAMRKDVISPSIDEFYYLS
                     RTTLVKDEQNFDKFDRAFGEYFKGIESVAGVEFDVPLEWLLKQAELNLSPEEKAMIEA
                     MGGWQKLMETLKKRLEEQKGRHQGGNKWIGTAGTSPFGAYGYNPEGVRIGQDGSRSRS
                     AVKVWDAREYRNLDDSVELGTRNIKVALRRLRKFAREGAPEELDLDTTIDKTARNAGY
                     LDLSMRPERHNAVKVLMFLDIGGSMDDHVKLAEELFSAAKTEFKHLEYFYFHNCLYDH
                     VWKDNRRRHSERTRTFDIMHKYGHDYKLVFVGDATMSPYEILQPGGSIEYSNDEAGAV
                     WLKRMLELYPRAVWLNPEPEEIWPYRQSIGILREIMGGRMYPTTIQGLERAMRALVK"
     gene            complement(1883..2725)
                     /locus_tag="VEM33_08470"
     CDS             complement(1883..2725)
                     /locus_tag="VEM33_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYS51901.1"
                     /translation="MRFKGSESYVSTDDLTLAVNAAITLQRPLLVKGEPGTGKTMLAV
                     EVAKALGLPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDPRVHEIRNYIVKGMLWQ
                     AFTSETPVVLLIDEIDKADIEFPNDLLRELDRMEFFVYETKELVKATQRPVVFITSNN
                     EKELPDAFLRRCFFHYIRFPDKETMEKIVEVHFPGLKKSLLRDALEMFFEVREVPGLK
                     KKPSTSELLDWLKLLLAEDIPPDALRAKDRKTIIPPLHGALLKNEQDVHLFERLIFMS
                     RQRS"
     gene            2746..3468
                     /locus_tag="VEM33_08475"
     CDS             2746..3468
                     /locus_tag="VEM33_08475"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYS51902.1"
                     /translation="MRLIVYNRTVRALFPLLLCVAVSAGAATVAIDVGHFIEEPGATS
                     ARGRPELDFNRDLAAEIESASRSRGLKTLLIGYDGFMSQLTARTAAARNADLFLAVHH
                     DSVQPQFLETWEFDLVERPFSDRYSGFSLFVSRKNAAVKRSLSCASAIGEALRHAGFS
                     PSLYHADPIPGENKPFADRRNGVHYYDNLIVLKTARTPAVLLEAGVIVNRDEELKVQS
                     EETRKRIAAAVAQGVERCLARF"
     gene            3539..3862
                     /locus_tag="VEM33_08480"
     CDS             3539..3862
                     /locus_tag="VEM33_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYS51903.1"
                     /translation="MRISPVLLLLACLPVHAQTVGNAEAAKGKNSMCIGCHGIPMYKT
                     AFPEVYSVPMIAGQAPDYIVKSLQAYRAGDRSHPSMQGIAKSLSDQDMADLAAYYGGA
                     GKEVK"
     gene            3859..4179
                     /locus_tag="VEM33_08485"
     CDS             3859..4179
                     /locus_tag="VEM33_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYS51904.1"
                     /translation="MTRFLVVVLACFSAQAFAAGDADSGKLKSQVCAACHGPDGNSPT
                     GPDFPRLAGQHYDYLLKALRDYRSGARRDPIMGAQVGGLNSQDLADLAAFYSRQRGSL
                     HVLR"
     gene            complement(4193..4828)
                     /gene="nth"
                     /locus_tag="VEM33_08490"
     CDS             complement(4193..4828)
                     /gene="nth"
                     /locus_tag="VEM33_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYS51905.1"
                     /translation="MNPQKRRKIFERLRSANPHPTTELTHRTPFELLVSVVLSAQATD
                     KSVNLATERLYRVADTPQAMLALGMEGLIGHIRTIGLYRSKAKHILETCRLLVERHGG
                     KVPESREALEALPGVGRKTANVILNTAFGHPTIAVDTHIFRVANRTGLAPGKDVREVE
                     ERLLKFVPEEFRKDAHHWLILHGRYVCKARKPECPACIIRDLCEFRKKTKA"
     gene            complement(4825..5445)
                     /gene="rsxB"
                     /locus_tag="VEM33_08495"
     CDS             complement(4825..5445)
                     /gene="rsxB"
                     /locus_tag="VEM33_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HYS51906.1"
                     /translation="MSGLVRRIDALLPQTQCTKCGYPACRPYAEAIARGEAEINQCPP
                     GGDAVIRGLAALLGREARPLNPRNGIEQPRRVALIDEARCIGCTLCIQACPVDAIVGA
                     AKLMHTVVTELCSGCDLCVAPCPVDCIAMVPATGEDAVWDTALAHAARERFERRRSRL
                     ELERRERAERLAARALRAKEHPGAEKKRVTIQAAIERARARRRRQA"
BASE COUNT          900 a         1763 c         1759 g         1052 t
ORIGIN      
        1 gctccgagga cgcgccgatc gagtcccggc attcctggga ttgcctcaat ctcttcagca
       61 gctgtttcct cttcaattta ccgaggcacg cgcatcatca tctgcgcgca ggcgacccgt
      121 tctgggagct gaaggccgat cccggcgcct tggcgctcga gtccgggtac atggccgcca
      181 tcctgctcgc gatggtgccg ccggtgtgga cccggatgat ggcgggggcg ctgcgttact
      241 gggacgagaa gctggcgacc cgggaagagc gggctctgat ccgcgcagcg ggcgcggcgc
      301 gctgaaacgc ttatttctcg ctggtgcagg cccacacctt gaccgcgcaa tcgccgccgc
      361 acttcttgag cgccttcatc tcggcgatct gccgggacgc gccggtgtct gaagacgtgc
      421 gggtcggctt agccgcgacc gccgcgcatg cgtcccgaaa ggtccggatc agttcgcagt
      481 tcgatccgca ctgacggaag gcatccgtct cggcctcgcg cttggtcttc cggtcgacgg
      541 cgtatccgta ggaacccgac ttggagttgt aagcgatagc gccccaaagc tgccgcttgc
      601 cctggcgcgc ggcagcggcg tcgatcgaga aagaggcgaa ggcgacgagc agtccgacga
      661 cgacggtgat ccgacgcatg gctcgaccta tttcaccaga gcgcgcatcg cgcgctccag
      721 tccctggatg gtggtgggat acatgcggcc tcccatgatt tctctgagta tgccgatcga
      781 ctggcggtaa ggccagatct cctccggttc gggattgagc cagaccgccc tggggtacag
      841 ctccagcatg cgcttcagcc acaccgcacc cgcttcgtcg ttggagtact cgatcgagcc
      901 gcccggctgc aggatctcgt acgggctcat ggtggcgtcg ccgacgaaga cgagcttata
      961 gtcgtggccg tacttgtgca tgatgtcgaa ggtccgggtg cgctcggagt gccggcggcg
     1021 gttgtccttc cacacatggt cgtagagaca attgtggaag tagaaatact ccaggtgctt
     1081 gaattcggtc ttcgccgccg agaagagctc ctcggcgagc ttcacgtggt cgtccatcga
     1141 gccgccgatg tcgaggaaca tcagcacctt gacggcgttg tggcgctcgg ggcgcatgct
     1201 caggtcgaga tatccggcgt tgcgcgcggt cttgtcgatg gtggtgtcga ggtcgagctc
     1261 ttccggcgcg ccctcgcgcg cgaacttgcg caagcgccgg agcgccacct tgatgttgcg
     1321 cgtgcccagt tccaccgagt cgtcgaggtt gcggtactcg cgtgcatccc acaccttgac
     1381 cgcgctcctg ctgcgcgagc cgtcctggcc gatacgcacg ccctcggggt tgtagccgta
     1441 ggcgccgaaa ggggaggtgc ccgcggtgcc gatccacttg ttgccgccct ggtgccggcc
     1501 cttttgctcc tcgaggcgct tcttgagcgt ctccatgagt ttttgccagc cgcccatcgc
     1561 ttcgatcatc gctttctctt cgggcgaaag gttcagctcg gcctgcttga gcagccattc
     1621 gagcggcacg tcgaactcga ctccggcgac cgactcgatt cccttgaaat actcgccaaa
     1681 ggcgcggtcg aacttgtcga aattctgctc gtccttcacc agcgtcgtgc gcgagaggta
     1741 gtagaactcg tcgatcgagg gcgagatgac gtctttccgc attgcctcca ggagcgtcag
     1801 ccactccttg atcgaaaccg ggatcttgtg gtccttgagc ttgacgaaga atccggagag
     1861 catgggcgcg aaggcggcaa ggctacgatc gctggcgcga catgaagata aggcgttcga
     1921 agagatgcac atcctgctcg ttcttgagca gtgccccgtg gaggggcggg atgatcgtct
     1981 tgcggtcctt ggccctcagg gcgtcgggcg ggatgtcctc ggcgagcagg agcttcagcc
     2041 agtcgagcag ctccgaggtc gagggcttct tcttgagtcc gggcacttcg cgcacctcga
     2101 agaacatctc gagcgcatcg cgaagcagcg atttcttgag ccccggaaag tgcacttcca
     2161 cgatcttctc catcgtttcc ttgtcgggga agcgtatgta gtggaaaaaa cagcgccgca
     2221 agaaggcgtc gggcagctcc ttttcgttgt tcgacgtgat gaagacgacg gggcgctgcg
     2281 tcgccttcac cagctccttc gtctcgtaaa cgaagaattc catccgatcg agctcgcgca
     2341 ggaggtcgtt gggaaactcg atgtcggcct tgtcgatctc gtcgatgagg agcacgaccg
     2401 gcgtctcgga agtgaaagcc tgccacagca tgcccttgac gatgtagttg cggatctcgt
     2461 gcacgcgcgg gtcgccgagc tgggaatcgc gcaggcggct caccgcgtcg tattcgtaca
     2521 agccctgctg ggccttggtc gtggacttga tgtgccattc gaggagcgga agtccgagcg
     2581 ccttggccac ctccacggcg agcatggtct tgccggtgcc cggctcgcct tttaccagca
     2641 gagggcgctg cagcgtgatc gccgcgttga ccgcgagcgt gaggtcgtcg gtggagacgt
     2701 aagattcaga gcctttgaat cgcatcagtc tgccttcgaa tcgggttgcg cctcattgta
     2761 tacaatcgca ccgtgcgtgc attgtttccg ctgctgttgt gcgtcgccgt ctccgctgga
     2821 gcggcgaccg tcgccatcga cgtgggacat ttcatcgagg agccgggggc gaccagcgcc
     2881 cgcggacgcc ccgagctcga cttcaaccgc gatctcgccg cggagatcga atccgcctcg
     2941 cgcagccgcg gattgaagac cttgctcatc ggctacgacg gcttcatgtc ccagctcacg
     3001 gcgcgcacgg cggcagcacg gaacgcggac cttttcctgg ccgttcacca cgactcggtt
     3061 cagccgcaat ttctcgaaac ctgggagttc gacctcgtgg aacgtccgtt cagcgaccgc
     3121 tattccggtt tttccctgtt cgtatcgcgc aagaacgcag cggtgaaacg aagcctcagc
     3181 tgcgcctcgg cgatcggcga ggcactgcgc catgcgggat tctccccttc gctctatcac
     3241 gccgatccga tcccggggga gaacaagccg tttgccgacc gcaggaacgg cgtgcactac
     3301 tacgacaacc tcatcgtgct caagaccgcg agaactcccg ccgtcctcct cgaagcgggt
     3361 gtgatcgtca accgcgatga ggagctgaag gttcaatcgg aggaaacgcg caagcgaatc
     3421 gcggcggcgg tggcacaggg agtggagcgc tgtttggccc gtttttgacc ctcgcggagc
     3481 gcatcgtata gaatcggccg accttctcgc ggtaccagac gcctttcgga aacgaagagt
     3541 gagaatatcc ccggtccttc tgctcctggc atgcctcccg gtgcacgcgc aaaccgtcgg
     3601 aaacgccgag gccgcgaagg gaaagaactc gatgtgcatc ggctgccatg gcatcccgat
     3661 gtacaagacc gcgttcccag aggtctactc ggtgcccatg atcgcgggac aggcgcccga
     3721 ctatatcgtc aagtcgctgc aagcctaccg ggcgggcgat cgcagccatc cgagcatgca
     3781 gggaatcgcg aaaagcctct ccgaccagga catggccgac ctcgccgcct actacggcgg
     3841 cgctggcaag gaggtcaaat gacgaggttc ctggtcgtcg tgctcgcgtg cttcagcgcg
     3901 caagccttcg cggcgggcga tgcggactcc ggaaagctga agtcccaggt ctgcgccgcc
     3961 tgccacgggc ccgacggaaa cagcccgacg ggtccggatt ttccgcggct cgccgggcag
     4021 cactacgact acctgctgaa ggccttgcgg gactacaggt cgggcgcgcg cagggacccg
     4081 atcatgggcg cgcaggtcgg cggcctcaac tcgcaggacc tggccgatct cgcggcgttc
     4141 tattcgcgcc aaagaggctc gctgcacgtt cttcgctgaa gttcggcggg cttcaggctt
     4201 tcgtcttttt ccggaattcg cacaggtccc ggatgatgca ggccgggcac tcgggttttc
     4261 gcgccttgca cacatagcgc ccgtgcagga tcagccagtg gtgcgcgtcc ttgcggaatt
     4321 cctccgggac gaatttcagc agcctctctt ccacttcgcg cacgtccttg cccggcgcga
     4381 ggccggtgcg gttggcgacg cgaaagatgt gcgtgtcgac tgcgatggtc ggatgcccga
     4441 aggcggtgtt gaggatcacg ttggcggttt tcctgccgac accggggagc gcttcgagcg
     4501 cctcgcgcga ttcgggcacc ttcccgccgt ggcgctcgac gagcagccgg caggtctcga
     4561 gaatgtgctt cgccttggag cgatacaggc cgatcgtgcg gatatgcccg atcaatccct
     4621 ccatgcccaa ggcgagcatg gcctgcggag tgtcggccac gcgatagagc ctttcggtcg
     4681 caagattcac gctcttgtcg gtggcctgcg ccgacaacac caccgacacc aacagctcga
     4741 agggagtccg gtgcgtgagc tcggtcgtcg ggtggggatt ggccgagcgc agccgctcga
     4801 aaatcttccg gcgcttttgc ggattcacgc ctgccgccgc ctccgcgcgc gcgcgcgttc
     4861 gatcgcggcc tggatcgtca cgcgcttctt ctccgcgccg ggatgttctt tcgctctcag
     4921 cgcccgcgcc gccaggcgct cggcgcgctc cctgcgctcg agctccagcc gcgaccggcg
     4981 ccgctcgaag cgctcgcggg ccgcgtgcgc gagggccgtg tcccagaccg catcctcgcc
     5041 cgttgcaggc accatcgcta tgcaatccac ggggcagggc gcgacgcaca ggtcgcatcc
     5101 ggagcacagc tccgtcacga ccgtgtgcat cagcttcgca gccccgacga tcgcatcgac
     5161 cgggcaggcc tggatgcaca gagtgcatcc gatgcagcgc gcctcgtcga tcagcgcgac
     5221 gcggcgcggc tgctcgatgc cgttccgcgg attgaggggc cgcgcttcgc ggccgagcag
     5281 cgccgccagc ccgcgaatga cggcgtcgcc tccgggcggg cactgattga tttcggcctc
     5341 tcctctcgcg atcgcttccg cgtacggcct gcacgcgggg tagccacact tcgtgcactg
     5401 cgtctgcggc agcagcgcgt cgatccgccg gacgagtccg ctcatcgagc tgtcgccagg
     5461 ccgcggacga tttc
//
LOCUS       DASPZD010000438         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1733435, whole genome shotgun sequence.
ACCESSION   DASPZD010000438 DASPZD010000000
VERSION     DASPZD010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733435"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VEM33_08500"
     CDS             complement(<1..946)
                     /locus_tag="VEM33_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYS51907.1"
                     /translation="MVPARAAFFALALASSVQANPHEFTLANGLRLIVKEDHRAPTVV
                     HQVWYRAGSMDEAPGTTGVAHVLEHMMFKGTRRFGPGEFSRLLAEAGGRENAFTSTDF
                     TVYHEQVHRDRLPLAMQLEADRMANLVIRPEEFAREIKVVMEERRRSYEDRPRSLLIE
                     NLLATAFAAHPYKWPTIGWMQDLESMSWRDARAWYKHWYTPNNAVVVVVGDVEPQKVL
                     ALARRYYGVLPQKATPARKTLGEPVQRGLKRVTVKAPAELPYVRLGYKAPVLRDMAKD
                     WEPFAISLLAELLGGSDAARLHQVLVRERRIATSVSADY"
     gene            1014..1946
                     /gene="ftsY"
                     /locus_tag="VEM33_08505"
     CDS             1014..1946
                     /gene="ftsY"
                     /locus_tag="VEM33_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYS51908.1"
                     /translation="MLELSSKAARGEVSWATRLRSGLGKTRSRLSGLFTDRSVDPALF
                     EEIETALLASDAGAEATRFLLAALRERASQGATAGQLKTALRALLLELLAPLEQTLDL
                     SGKKPCIVMLAGVNGSGKTTSIGKLARYFQDQGRSVLLAAGDTFRAAAREQLAVWGEK
                     NGVAVVAQSGGDPGAVVFDAIAAARSRGIDLVLADTAGRLPTQLHLMEEISRIKRAIA
                     KAEPGAPHEVWLVLDANTGQNALKQVEAFDDALGLSGLILTKLDGTAKGGVVCAIALA
                     RSRRPLPLRFVGVGEGIDDLRPFAAGTFVSALLD"
     gene            1955..>2115
                     /locus_tag="VEM33_08510"
     CDS             1955..>2115
                     /locus_tag="VEM33_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYS51909.1"
                     /translation="MAIIALRQVSKRYPGGFEALKAVSFTIEPGEMLFLTGHSGAGKT
                     TLLKLIAAI"
BASE COUNT          344 a          699 c          704 g          368 t
ORIGIN      
        1 cgtagtccgc gctcacggaa gtggcgatgc gccgctctcg caccagtacc tggtgcaggc
       61 gcgccgcgtc gcttccgccg aggagctctg cgagaagcga aatggcgaac ggctcccagt
      121 cctttgccat gtcgcgcagc accggcgcct tgtatcccag gcgcacgtag gggagttccg
      181 ccggagcctt cacggtaacg cgcttcagcc cccgctggac cggttcgccg agcgttttgc
      241 gtgcgggcgt cgccttttgc gggagcacgc cgtaataacg ccgcgccaag gcgagcacct
      301 tctgcggctc gacgtcgccg accacgacga cgacggcgtt gttgggcgta taccagtgct
      361 tgtaccacgc ccgggcatcc cgccagctca tgctctccag gtcttgcatc cagccgatcg
      421 tcggccattt gtagggatgc gcggcgaaag ctgtcgcgag gagattttcg atgagaagcg
      481 aacgcgggcg gtcttcgtaa ctgcgccggc gctcctccat caccaccttg atctcgcgcg
      541 cgaactcctc cgggcggatc acgaggttcg ccatgcggtc ggcctcgagc tgcatcgcga
      601 ggggcaggcg gtcgcgatgc acctgctcgt ggtagacggt gaaatcagtg gaagtgaaag
      661 cgttctcgcg cccgcccgcc tccgcgagga gccgcgagaa ctcgcccggg ccgaagcgcc
      721 gggtcccctt gaacatcatg tgttccagca cgtgagcgac accggtggtg ccgggcgcct
      781 cgtccatgct gcccgcccgg taccatacct ggtgaacgac ggtgggcgcg cggtggtctt
      841 ccttcacgat caggcgcaag ccgttcgcca gagtgaactc gtgcgggttc gcctgcacac
      901 tcgaagcgag cgcgagggcg aaaaaggcgg cgcgtgcggg aaccattgcg acggatgcta
      961 aaatcgaact gctattctat agcggttctt tttccggttc ttttttctcg cccatgctcg
     1021 aattgtcttc caaggctgcc cgcggggagg tctcatgggc cacacggctc agaagcggcc
     1081 tcggaaagac gcgctcgcgg ctctcgggac tcttcaccga ccgaagcgtc gatcctgcgt
     1141 tgttcgagga gatcgaaacc gcgttgctcg cgagcgatgc cggggccgag gcaacgcggt
     1201 tcctgctcgc ggctctgcgc gagcgcgcga gccagggcgc gaccgcgggg cagctgaaaa
     1261 cagcgctacg ggcccttctg ctcgagctgc tcgcgccgct cgagcagacg ctcgatttgt
     1321 ccggcaagaa gccgtgcatc gtgatgctcg cgggagtcaa tggctcggga aagacgacct
     1381 cgatcggcaa gctcgcaagg tacttccagg atcaaggccg ctcggtgctg ctcgccgcgg
     1441 gggacacttt tcgcgccgcg gcgcgcgagc agctcgccgt ttggggcgag aaaaatggcg
     1501 tcgcggtcgt cgcccagtcg ggcggcgatc cgggagcggt cgtgttcgat gccattgccg
     1561 cggcgcgctc gcgcggaatc gacttggtcc tcgccgacac ggccgggcgg ctgcccacgc
     1621 agctgcacct catggaggag atcagcagga tcaaacgagc catcgccaag gcggaacccg
     1681 gcgcgcctca cgaggtatgg ctcgtgctcg acgccaacac gggacagaac gccctcaaac
     1741 aggtcgaggc cttcgacgac gcgctcggcc tctcgggatt gattctcacc aagctcgacg
     1801 gcaccgccaa gggcggcgtg gtgtgcgcca tcgcgctggc gcgatcgcgg cgtccgctgc
     1861 cgctcagatt cgtgggcgtc ggcgaaggga tcgacgatct gcgcccgttc gccgccggca
     1921 cgttcgtttc ggcgcttctc gattgaccgg ccgcgtggcg atcatcgcgc ttcgccaggt
     1981 cagcaagcgc tatcccgggg gattcgaggc gctcaaggca gtcagcttca ccatcgagcc
     2041 cggggagatg ctgttcctca ccggccactc cggtgccggc aagacgacgc ttctcaagct
     2101 gatcgccgcc atcga
//
LOCUS       DASPZD010000439         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_409398, whole genome shotgun sequence.
ACCESSION   DASPZD010000439 DASPZD010000000
VERSION     DASPZD010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409398"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..277
                     /locus_tag="VEM33_08515"
     CDS             44..277
                     /locus_tag="VEM33_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYS51910.1"
                     /translation="MQVSKWGNSLAIRLPAAVVEALGLKEGDDIILHAAGKRSLEIEK
                     APSTKELLARLRKFRGRLPEDFKFDRLEANARR"
     gene            264..674
                     /locus_tag="VEM33_08520"
     CDS             264..674
                     /locus_tag="VEM33_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYS51911.1"
                     /translation="MRGASVFFDTSVLLYLLSEDDRKADRVEALLQDSGNVSVQVLNE
                     FTAVLRGKLGMSFAEIREILATVRAVCRTHPLMVETHDRAIEIAERYKFSVYDSVIVA
                     SAYLAGCKTLYAEDLQHLQVIEGQLTLINPFLPR"
     gene            complement(737..1192)
                     /locus_tag="VEM33_08525"
     CDS             complement(737..1192)
                     /locus_tag="VEM33_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HYS51912.1"
                     /translation="MPQFEGLGLVGALLDVNVLIALLDSDHTLHGAAMSWFAKHAREG
                     WASCPITQNGCVRIMSHPNYPNALPVQAVMKRLANACEEDIHEFWSDEVSLLDADVVD
                     STRIHGPRRLTDIYLLALAVEHEGRFVTFDAGTPLAAARKATKRNLVVL"
     gene            1569..1829
                     /locus_tag="VEM33_08530"
     CDS             1569..1829
                     /locus_tag="VEM33_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51913.1"
                     /translation="MKTTVEIPDPLFRKAKARAAERGQTLKEFMSEALQEKLLTKAGN
                     ARTGEPEWMKGFGKLHHLHKETARIQKRVDKIFEVIEPEDRA"
     gene            1832..2203
                     /locus_tag="VEM33_08535"
     CDS             1832..2203
                     /locus_tag="VEM33_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYS51914.1"
                     /translation="MDTNALSAFVDGDSGVGDVLRRHPRAAIPVIVLGEFRYGIVECR
                     HRAQYEAWLTSYLPQFDILPITEETATAYASLRIALKRSGRPIPANDAWIAALAIQHR
                     LPVLSRDQHFDAVPDLDRESW"
     gene            complement(2258..4054)
                     /locus_tag="VEM33_08540"
     CDS             complement(2258..4054)
                     /locus_tag="VEM33_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51915.1"
                     /translation="MGGPSPFDRTCALARRRAAAPLFALFVFPGLACASIATSDAGAG
                     AEAAALASTERAATDGVGSGVERSSIRPPSYGPAGETPGIPSDAELEGAGAMIGEILI
                     DNQNIFNLEDPKDDVKLFRLANHLHSRTRKAIVRDQLLFRSGERYSRRLIDESERILR
                     AESYFYDAWIRPVRYHDGQVDLRVTTRDVWTLNPGFNFGRSGGSNSTGVQLEEINLLG
                     TGAGVKLAHTKDVDRTKSQLEVSDMHAFGTWTAVDVNYADLSDGRMRDLRLNRPFYAL
                     DTRWAAGVAGMDDVQTDSLYDRGQIIDRFQERRQLVQVYGGWSNGLQNGWVRRWSSGV
                     TYDEHRFEPVSTWTGVTAVPEDRRFLYPWIQFDLVQDDYLKLWNHDQIARTEDFYLGT
                     TASVRLGWADAAFGSSRSALMLQSSAGRGFRDGGSSTLLLAGTLTGRLENGEPRNVLL
                     AGSVRYYVEQNKNWLFFTTLQATKGWRLDLEDQILLGGDNGLRGYPLRYQDGTARALW
                     TVEQRFFTDWYPFRLFRVGAAVFFDTGRTWGAAPLAAPSLGPLRDAGFGLRFGNSRSG
                     LGNVVHVDLAFPFNGDSSIKRVQFLVQTEQRF"
BASE COUNT          814 a         1395 c         1388 g          780 t
ORIGIN      
        1 gcattagata tactcaagaa caatagtata tccagaggtg cccatgcaag tatcgaaatg
       61 gggcaatagc ctggcaatcc gacttcccgc cgccgttgtg gaagccttgg gactcaaaga
      121 gggcgatgac atcatcctcc acgctgcggg aaagcggtca ctcgaaatcg agaaagcgcc
      181 cagcaccaag gagcttctgg cgcgccttag gaaattccgg ggccgattgc ctgaggattt
      241 caaattcgat cgtctagagg cgaatgcgcg gcgctagcgt cttcttcgac accagcgtcc
      301 tgctctacct cctgtcggag gatgacagga aggcggaccg cgtagaagcg ctgctgcagg
      361 actccggcaa tgtcagcgtt caggtgctca acgagtttac tgccgtcttg agaggcaagc
      421 tcggtatgtc attcgcggaa atccgcgaaa ttctcgcgac ggttcgagcc gtttgccgca
      481 cccatccgct gatggtcgag acgcatgacc gcgccatcga aattgcggaa cgctataagt
      541 tctccgtgta cgactcggtc attgtcgcgt ccgcgtatct cgcaggctgc aagaccctct
      601 acgctgagga tctacagcat cttcaggtca tcgaggggca attgaccctc atcaatccct
      661 tcctgccgcg ctaggcatcc aagggccgca aacgaaggcc cggcggggcg agcgcctggt
      721 ccacccaaac gaccgcctac agcactacca gattgcgctt ggttgccttg cgagccgcgg
      781 caagaggagt cccggcgtcg aacgtgacga atcttccttc atgctccacg gcgagggcga
      841 ggaggtatat gtcggtaagt cgccgggggc catggattcg cgtcgagtca acaacgtccg
      901 catccagcaa gctgacttca tctgaccaga actcgtggat atcttcctca cacgcgttgg
      961 caagacgctt catgacagcc tggaccggta atgcgtttgg atagttcgga tgcgacatga
     1021 tacggacaca accgttttgg gtgatgggac aggaagccca gccttctcgc gcgtgcttcg
     1081 caaaccaact cattgccgcc ccgtgcagcg tgtgatcgga gtcgagcaat gcgatcagca
     1141 cgttgacgtc caacagcgcg cccactaatc caagcccttc gaactggggc aaccgacggg
     1201 ccggagagca cctggccggt gtgtgcggcg cttctctcgc cgggggttaa tagggatcat
     1261 cttcgcgaag cttatcgatc agttcattcg tgacgatccc gccgcgcgga gcgaacggcc
     1321 gaagaccgta gattgccttt ggttccttta cggaaggcgg ctccgggggg gacgtcaggg
     1381 cacgccgggc caagtccgaa atcatctctc cgatagtctt tctttcgcgc tgcgcccgct
     1441 cctttgcggc ttgaagcacg tcatcggcaa tgtcgagtgt cgttcgcatg catcagatgt
     1501 tacagcatca cgcatctaat ggcgagtcgg cgatcgatcg ctccccgtaa atacggtaat
     1561 atggttctat gaagacaacc gttgaaatcc cggacccctt gtttcgcaag gccaaggcgc
     1621 gcgcggccga gcgcggtcag acgcttaagg aattcatgtc tgaggcactg caggaaaagc
     1681 tcctgaccaa agcgggcaac gcgcgcactg gagagcccga gtggatgaag ggattcggaa
     1741 aactccacca cttgcacaaa gagactgccc gaatccagaa gcgcgtggac aaaatcttcg
     1801 aggtgattga accggaggat cgcgcctgat tctggacacg aacgcacttt cggcgttcgt
     1861 cgacggcgat tccggggttg gcgacgttct acgtcgtcac ccgcgagccg ccattcccgt
     1921 catcgttctg ggggaattcc gctatggcat cgtggagtgc aggcaccgtg cgcaatacga
     1981 agcgtggcta acctcctatt tgccccagtt cgatatcctg ccaatcacgg aagagactgc
     2041 aacggcttat gcgtcgctgc gaatcgccct caagcgatcc ggccgcccca tcccggccaa
     2101 cgatgcctgg atcgccgccc tcgccattca gcaccgtctt ccagtgctca gtcgcgacca
     2161 gcacttcgac gccgtcccgg acctcgaccg ggagagctgg tagggcttag gacgccccct
     2221 cttatccgga tcaacccgaa ggctcggccg cgcgctttca gaagcgctgc tcggtctgca
     2281 cgagaaactg cacccgcttg atgctgctgt cgccgttgaa ggggaatgcc aggtcgacgt
     2341 gcacgacgtt gcccaggccc gagcgggagt tgccgaagcg cagaccgaag cctgcgtccc
     2401 tcaagggccc gaggctcggc gcggccagcg gcgcggcgcc ccaggtgcgg ccggtgtcga
     2461 agaacaccgc ggcgcccacg cgaaacagcc ggaacgggta ccagtcggtg aagaagcgct
     2521 gctccaccgt ccacagcgcc cgtgccgtgc catcctggta acgcagcgga tagccgcgca
     2581 gcccgttgtc gccgccgagc aggatctggt cctcgagatc cagccgccac cccttggtcg
     2641 cctgcagcgt cgtgaagaac agccagttct tgttctgttc gacgtagtag cgcaccgagc
     2701 ccgcgagcag cacgttgcgc ggctcgccgt tctcgagccg gccggtcagg gtgccggcga
     2761 gcagcagcgt cgagctgcca ccgtcgcgga aaccgcggcc cgcggagctc tgcaacatga
     2821 gcgcgctgcg gctcgagccg aacgccgcgt ccgcccagcc gaggcgcaca ctggcggtgg
     2881 tgccgaggta gaagtcctcg gtgcgcgcga tctggtcgtg gttccagagc ttcagataat
     2941 catcctgcac caggtcgaac tgtatccacg ggtacaggaa gcgccgatcc tcggggaccg
     3001 cggtcacgcc ggtccaggtg ctgaccggct cgaagcggtg ctcatcgtag gtgacgccgc
     3061 tgctccagcg ccgaacccag ccgttctgca gtccgtttga ccagccgcca tagacctgca
     3121 cgagttggcg ccgctcctgg aaccgatcga tgatctgccc gcggtcgtac agggaatcgg
     3181 tctgcacatc atccatgccc gcaactccgg ccgcccagcg ggtgtcgagc gcgtagaagg
     3241 ggcggttcag cctgagatcg cgcatccggc cgtcgctgag gtccgcgtag ttcacatcca
     3301 ccgcagtcca ggttccgaag gcgtgcatgt cggatacctc cagctgagac ttggtgcggt
     3361 cgacgtcctt ggtgtgggcc agcttgaccc cggcccccgt gccgagcagg ttgatctcct
     3421 ccagctgcac gcccgttgaa ttgctgccgc cactgcgccc gaagttgaac ccgggattga
     3481 gggtccacac atcgcgcgtg gtgacgcgca ggtcgacctg gccgtcgtga tagcgcaccg
     3541 ggcggatcca ggcgtcgtag aagtagctct cggcgcgcaa tatgcgttcg gactcatcga
     3601 tcaggcggcg cgaatagcgt tcgcccgacc tgaacagcag ctggtcgcgg acgatcgcct
     3661 ttcgggttcg gctgtgcagg tggttcgcga gccggaacag cttgacgtcg tccttcgggt
     3721 cctcgagatt gaagatgttc tggttgtcga tcaggatctc accgatcatc gcaccggcgc
     3781 cctcgagctc ggcatcggac gggatgcccg gcgtctcccc ggccgggcca taggagggtg
     3841 gtcggatcga ggagcgctcg accccggagc cgacgccgtc ggtggcggcg cgctcggtgc
     3901 tcgcgagcgc cgcggcctcg gcgcctgcgc ccgcgtccga cgtggcgatt gacgcgcacg
     3961 ccagcccggg aaagacgaac agggcgaaga gcggcgccgc cgcgcggcgc cgggcaagtg
     4021 cgcaggtgcg gtcgaacggc gaaggacctc ccatggccgc atcacacccc gggtcgtcag
     4081 tgcgcgagtc tgtgcatccg ggcattctgc gctgtcgctc tcggggcacc ggtgccacag
     4141 gtagcagttc gcaagccccg gccggaaccg tgactcaccg caagcgccgc gacgcccggg
     4201 cttgacaacc cggctcagtt ctcttagcga agggaaggac gtatgacaag ccagtggccg
     4261 cgccatgcgc ttgtaggctg ggcactgacc ctgctcgggg cgggcactgc cgggtcggcg
     4321 ggcgcggcgg gtgcggcggg tgcgggcact gccggcgagc acgcgccgac ggcgctg
//
LOCUS       DASPZD010000440         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1301954, whole genome shotgun sequence.
ACCESSION   DASPZD010000440 DASPZD010000000
VERSION     DASPZD010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301954"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1279)
                     /gene="ychF"
                     /locus_tag="VEM33_08545"
     CDS             complement(185..1279)
                     /gene="ychF"
                     /locus_tag="VEM33_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HYS51916.1"
                     /translation="MSLKCGIVGLPDVGKSTLFNALTKAGIAAENYPFCTIDPNIGIV
                     EVPDPRLAGIAAIAKPQKVVAAAVEFVDIAGLVAGDSKGEGLGNQFLANIRECDAIAH
                     VVRCSEDPNVVHVAGKVDPVSDIETIHTELALADLATLDRTYARYAKVAKTGGDKEAQ
                     RIVAVLDKVRPALDQARPARSVALSKEEQAVLKPLFLMTSKPEMFIANLAEHGFENNP
                     LLAQVEEYAKRHDSPVVPICAALEAQIADLSDEDKQVFLADMGMKEPGLNRLIRTGYA
                     LLGLQTYFTAGPKEVRAWTVPVGATAPQAAAVIHTDFERGFIRAEVIAYADYIACKGE
                     QGAKEAGKMRLEGKDYVVRDGDVIYFRFNV"
     gene            complement(1279..1857)
                     /gene="pth"
                     /locus_tag="VEM33_08550"
     CDS             complement(1279..1857)
                     /gene="pth"
                     /locus_tag="VEM33_08550"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYS51917.1"
                     /translation="MHSLKLIAGLGNPGRKHERDRHNVGFWFASRLAAARRIELKAVA
                     KYHGLLGKLALASGDLWLLLPQTYMNVSGKSVGALARFYKIQPQEILVVHDELDFPPG
                     VAKLKLGGGVAGHNGLKDIAAQLGTHGFWRLRIGIGHPGNKDLVAGYVLTPPPAGERE
                     LIEAALDRCLEASGLLLDGDMEAAMLKLHTKS"
BASE COUNT          329 a          666 c          669 g          401 t
ORIGIN      
        1 acggaggcgt ttcggtcacg atcaccgtgc gcgccatcga tgtatccggg aatagcaact
       61 caaggtccgt gaccttcacc cagactcagt agttcgggaa agaaatatcg gtattgcaat
      121 cccggcacaa cgcccagctt cgcgctgggc gttggcgttt tgcgctccgg atagaacgct
      181 tgggtcagac gttaaatctg aagtaaatca catccccgtc gcgaacgacg taatcctttc
      241 cttccagtct catctttccc gcttccttcg cgccctgctc gcctttgcag gcgatgtagt
      301 cagcgtaggc gatgacttcg gcgcggatga agccgcgctc gaagtcggtg tggatcactg
      361 cggccgcctg gggcgcggtg gcgccgacgg gaacggtcca ggcgcgcact tccttcggcc
      421 cggcggtgaa ataggtctga aggccgagca gggcgtagcc ggtgcggatg aggcggttca
      481 acccgggttc cttcattccc atgtcggcga gaaagacctg cttgtcctcg tccgaaaggt
      541 cggcgatctg ggcctcgagc gccgcgcaga tcggcaccac gggtgaatcg tggcgcttgg
      601 catattcctc gacttgcgca agcagcgggt tgttttcaaa gccgtgctcg gcgaggttgg
      661 cgatgaacat ctcgggcttc gacgtcatca ggaacagagg cttcagcacc gcctgctctt
      721 ccttcgagag cgccacgctg cgcgcggggc gcgcttggtc gagcgccggg cgcaccttgt
      781 cgagaacggc aacgatgcgc tgcgcctcct tgtcgccgcc ggtcttggcg actttcgcgt
      841 agcgcgcata ggtcctgtcc agcgtcgcta gatcggcgag ggcgagctcg gtgtggatgg
      901 tctcgatgtc cgaaaccgga tcgaccttgc ccgcgacgtg gacgacgttc ggatcctcgg
      961 agcacctgac cacgtgcgcg atcgcgtcgc attcgcggat attggcgagg aactggttcc
     1021 ccaggccttc gcccttcgag tcgcccgcca ccagtcccgc gatgtccacg aactccaccg
     1081 ccgccgcgac gaccttctga ggcttggcga tcgcggcgat cccggcaagg cgcgggtcgg
     1141 gcacctcgac gatgccgata ttgggatcga tggtgcagaa cggatagttc tccgccgcga
     1201 tccccgcctt ggtgagggcg ttgaataggg tcgatttccc tacatcgggc agcccgacaa
     1261 tgccgcattt caacgacatc aggatttcgt gtgcagcttg agcatcgccg cttccatgtc
     1321 gccgtcgagc aggaggcccg aggcctccag gcagcgatcg agcgccgctt cgatcagctc
     1381 gcgctcgccc gcaggcggcg gtgtcagcac gtagccggcg acgaggtcct tgttccccgg
     1441 gtggccgatg ccgatccgca ggcgccagaa cccgtgcgtg ccgagctgcg ccgcgatgtc
     1501 cttgaggccg ttgtggccgg cgacaccgcc gcccagcttg agctttgcga cgcccggcgg
     1561 gaaatcgagc tcgtcgtgta cgaccaggat ctcctgcggc tggatcttgt agaaacgcgc
     1621 gagcgcgccg accgacttgc ccgagacgtt catgtaggtc tgcggcagga gcagccacaa
     1681 gtcgcccgag gccagtgcca gcttgccgag gaggccgtga tacttcgcga ccgctttcag
     1741 ctcgatgcgc cgggcggccg caaggcgcga ggcgaaccag aagccgacat tgtgccggtc
     1801 cctctcgtgc ttcctcccgg ggttccccag gccggcgatc agttttagag aatgcatgga
     1861 tgagaaaggg cgcgggattc gccgcgccct ttcgcgtaat ggcaacaggc gctacttctt
     1921 gtcgcctctg tcgcctctgt cgcctttgtc gcccttcccg cctttgccgc ccttctcctc
     1981 cgcagcgggc gcagcggctt cgggcgcctt ctgcttgagg gtgggcacgt cggctgcggc
     2041 aggaacggct tccgcagcag gcgcg
//
LOCUS       DASPZD010000441         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1733891, whole genome shotgun sequence.
ACCESSION   DASPZD010000441 DASPZD010000000
VERSION     DASPZD010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733891"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1055)
                     /locus_tag="VEM33_08555"
     CDS             complement(93..1055)
                     /locus_tag="VEM33_08555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51918.1"
                     /translation="MTKLAALIAASLLSTVALAQGYPNRPVRVIVPWPPGQATDIAAR
                     VVAEKLQQALKQPFIIDNRAGAGGSIGTDAAVKSAPDGYTLLAASSGPISIMPHLQKT
                     PYDPDRDFAPISLVALVPFALVTHPSFPATNAKDFIAHLRANPDKYTFSSSGTGASAH
                     LITELFNSMAQVKARHVPYKGSAPALTDIVGGQIGYTIETLAATIGHIKGGRLKTFGT
                     SFAQRTAGMPDVPSIAEAADLPAYDIGAWVGYTAPAGTSRETCVRLSGEIQKAMQASD
                     LKERFISLGMDAVSNTPDEMAAFMRAQQERYGSIIKSQNIKIEQ"
     gene            complement(1165..>2148)
                     /locus_tag="VEM33_08560"
     CDS             complement(1165..>2148)
                     /locus_tag="VEM33_08560"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HYS51919.1"
                     /translation="YEINCRMIDAFDITTRGWDVPVFSLPAVALAAGKLMKLPPAQLA
                     QAVGLAINDHIPMAQTRVQALSDWKGLADAEAGRNAVFAALLARAGLTGPAPIFEGNS
                     GFFKQISGPADLDVGAFGRRGVPFRIHQCGMKAYPAVVYAQTAVVAGVEVARKLGSLD
                     RIAAIEIATTKRGYQRAGSEPEKWTPDTRETADHSLPYITARAMFDGDITNDSYAPGK
                     LSEPRILAFMRKITVKEDPALTARVGAMVPTRVTAILADGQRISSEVDDVPGFPGRPM
                     TRAEVERKFRGNVGRRWPRERTDAVLRDLWALDRTQDIAALLGKLSVQSNS"
BASE COUNT          335 a          704 c          722 g          387 t
ORIGIN      
        1 cgccgcgacg gtctcgtctg cgaggtctcc cccgagatcc accccgagga tctcggcgcc
       61 aagcgcaccg gaaatgcgct tgacctccat gcctattgct cgatcttgat gttctggctc
      121 ttgatgatcg agccgtagcg ctcctgttgc gcccgcatga acgctgccat ctcatcgggc
      181 gtgttggaaa cggcgtccat gcccagcgag ataaaccgct ccttcaggtc cgacgcctgc
      241 atggccttct ggatctcgcc cgagaggcgc acgcaagtct ctcgcgaggt cccggccggc
      301 gcggtgtagc cgacccacgc gccgatgtcg taggccggaa ggtcggccgc ttccgcgatc
      361 gacgggacat ccggcatgcc ggcggtacgc tgcgcgaacg aggtgccgaa ggtcttcagg
      421 cgaccgccct tgatgtgccc gatggtcgcg gccagagtct cgatggtgta gccgatctgg
      481 ccgcccacga tgtcggtgag cgccggcgcg ctacccttgt agggtacgtg gcgcgccttg
      541 acctgcgcca tcgagttgaa gagctcagtg atgaggtgcg ccgatgcgcc cgtgcccgaa
      601 gacgagaacg tgtacttgtc cggattcgcg cgcaggtggg cgatgaaatc cttcgcgttc
      661 gtcgccggga acgacgggtg cgtgacgagc gcgaacggca ccagcgcgac caagctgatc
      721 ggggcaaagt ccctgtcggg gtcgtagggc gtcttctgca ggtgcggcat aatcgatatc
      781 ggcccgctcg acgccgcaag cagggtgtag ccgtcgggcg cgctcttcac ggctgcgtcg
      841 gtgccgatcg agccgccggc gcctgcgcgg ttgtcgatga tgaacggctg cttgagcgcc
      901 tgttgcagct tctcggctac cacgcgcgcg gcgatgtcgg tggcctgccc cggcggccaa
      961 ggcacgatga ctctcacggg ccggttcggg tagccttgcg cgagcgcgac cgtggaaaga
     1021 agcgatgcgg ctatcaatgc ggcaagcttc gtcatgtctc ctccagcgcg tgatttttac
     1081 cctcgttacg gccgatcatg cgctgggcgc acgaaagggg tcaagcactt gcatgcttat
     1141 cgcgcttggg agatccgcgg cacgctaaga gttcgactgc accgagagct tgccgagcaa
     1201 ggccgcgatg tcctgcgtgc gatcgagcgc ccacagatcc cgaagaacgg cgtccgtccg
     1261 ctcccggggc cagcgcctgc ccacgttgcc gcggaacttc ctttcgactt ccgctctggt
     1321 catcggccgc cccgggaaac ccggcacgtc gtcgacctcg ctcgagatgc gctgcccgtc
     1381 ggcgaggatc gcagtcacgc gcgtcggaac catcgctccg acgcgcgccg taagggcagg
     1441 gtcttccttc accgtgatct tgcgcataaa ggcgaggatg cgcggttcgc tgagcttccc
     1501 aggcgcatag ctgtcgttcg tgatgtcgcc gtcgaacatg gcgcgcgcgg tgatataggg
     1561 caggctgtga tcggcggttt cgcgggtgtc cggcgtccac ttctcgggct cgctcccggc
     1621 gcgctgatag ccccgcttcg tggtcgcaat ttctatcgcc gcgatgcggt cgagggagcc
     1681 cagtttcctc gcgacctcga cgccggccac gaccgcggtc tgcgcgtaga ccacggccgg
     1741 gtaagccttc atcccgcact ggtggatgcg aaacggcacc ccgcggcggc cgaaggcgcc
     1801 gacatcgaga tcggccggtc cggaaatctg cttgaagaaa cccgaattcc cttcgaagat
     1861 gggcgccggg ccggtcaggc cggcgcgcgc gagcagtgcc gcaaacaccg cattgcgccc
     1921 ggcttcggcg tcggcgagtc ccttccagtc cgacagcgcc tggacccggg tttgcgccat
     1981 cgggatgtgg tcgttgatgg cgagaccgac ggcctgcgcg agctgcgcag gagggagctt
     2041 catcagcttt cccgcggcaa gcgccacggc cggaaggctg aacaccggca cgtcccagcc
     2101 gcgggtcgtg atgtcgaagg cgtcgatcat gcggcagttg atctcgta
//
LOCUS       DASPZD010000442         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_169240, whole genome shotgun sequence.
ACCESSION   DASPZD010000442 DASPZD010000000
VERSION     DASPZD010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169240"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VEM33_08565"
     CDS             complement(<1..1198)
                     /locus_tag="VEM33_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYS51920.1"
                     /translation="MKRPYLPTWILLAVAAVALPGITHARDRDRFQLVEATIAEIHDS
                     FRSGTLTPEELVRMYLARVAAYDQAGPQLNSFMHVNEHALSAARALRVDDLDDDARGR
                     DEDWEADGRRKPLFGVPVILKDNIDTADMPTTAGSIALGGSIPAKDAFITRKLRHAGA
                     IILGKATLTEFANFISSTGMPTGYSSQLRLQLFQRGGDLTRAGYGFDPFDPRTDPRQA
                     FNDGRPALATGGSSSGPGIAVSANLATVGVGTETSGSILSPATQNLLVGIKPTVGLVS
                     RTGIVPITADQDTAGPMARTVTDAAKLLGVLAGFDPQDPATAACLRPGNCFTDYTRFL
                     KRGALRGARIAVPHFGYWTDSLNRVVLSVEQQRVMNDAIAVLRAEGAIVEDFHEIPTQ
                     QELFDFG"
     gene            1432..2040
                     /gene="gstA"
                     /locus_tag="VEM33_08570"
     CDS             1432..2040
                     /gene="gstA"
                     /locus_tag="VEM33_08570"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HYS51921.1"
                     /translation="MKLYYAPGACSMAPHVVLREAGYKFDLEKVDIPNKKTAGGEDYW
                     KINPKGYVPALKLDDGQVLTEVQVICQYLADHKADSGLAPKAGTMERYRLMEMLNFTA
                     SEVHKQIGALFNPKMTPDMKEVQLAYVERRLNTLEKLLAGKQYASGGKFSIADVYLFT
                     ILNWTGLHKIDLAKWPNIKAFQARIAARPKVQETMKAEGLVK"
     gene            complement(2063..>2432)
                     /locus_tag="VEM33_08575"
     CDS             complement(2063..>2432)
                     /locus_tag="VEM33_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51922.1"
                     /translation="HRIMNLCMAATYDPDPRAPLGFRVPLNLESGEAIEERWRKWRGH
                     DPINLVARYRDNLKSLRGIYIDCGWRDQYHIHYGSRILSARLAAAGIRHRYEEFDDDH
                     SDVDYRMDVSLPFLYKALKP"
BASE COUNT          433 a          768 c          816 g          415 t
ORIGIN      
        1 caccgaagtc gaacagctcc tgctgcgtgg gaatttcgtg gaagtcctcc acgatcgcgc
       61 cctcggcccg cagcaccgca atggcgtcgt tcatgacccg ctgctgctcg acgctgagaa
      121 cgacgcggtt caggctgtcg gtccagtagc cgaaatgcgg gacagcgatg cgcgcaccgc
      181 gaagcgcgcc cctcttcagg aatcgcgtgt agtcggtgaa gcagttgccc ggcctgaggc
      241 acgcggcggt ggcggggtcc tggggatcga acccggcgag gactccgagt agcttggcgg
      301 cgtcggtcac ggtacgcgcc atcggcccgg cggtatcctg atcggcggtg atcggcacga
      361 tcccggtccg gctcaccagg cccaccgtcg gcttgatgcc gaccaggagg ttctgggttg
      421 cggggctcaa gatcgatccc gatgtctcgg tgccgacccc cacggtggcg aggttcgcgg
      481 acacggcgat gcccgggccg gagctggagc cgccggtcgc aagcgccggg cggccgtcgt
      541 tgaacgcctg cctcggatcg gtgcggggat cgaacgggtc gaagccgtaa ccggcccgcg
      601 tcaggtcgcc gccgcgctgg aagagctgca ggcgcagttg cgagctgtag ccggtcggca
      661 tgccggtgga gctgatgaag ttggcgaact cggtgagggt cgccttcccg aggatgatgg
      721 ctccggcgtg gcgcagcttc cgggtgatga aggcatcctt cgccgggatc gatccgccga
      781 gcgcgatcga tccggccgtc gtcggcatat cggcggtatc gatgttgtcc ttgaggatga
      841 ccggcacgcc aaacaagggc tttctgcgcc cgtccgcctc ccagtcttcg tcccggccgc
      901 gagcgtcgtc atcgagatcg tcgactctca aggcgcgcgc ggcggagagg gcgtgctcgt
      961 tcacatgcat gaagctgttg agctgtggac cggcctgatc gtaggccgcg acccgtgcga
     1021 ggtacatgcg caccagctcc tcgggggtga gagtccccga acgaaacgaa tcgtgaatct
     1081 cggcgatggt ggcctcgacc agctgaaacc gatcgcggtc ccgggcgtgc gttattcccg
     1141 gcagagcgac cgcggcaact gcaaggagaa tccaggtggg caggtagggg cgtttcatgt
     1201 ttttctccct tcgcaaagga tgctgctgcg aggcgcggcg gcaaggaaat gatccggtcg
     1261 gctcgtgacc cgtacgtttc atccgcccgg atgccccgat cgcttcctgc gcgcccggac
     1321 atgcggtaga ctttctccgg caatccgttt tccgcgcgcc ttcggcggca agtcaccccg
     1381 ggcgccaagc aagatctccg tgccatccgt tcacaaggaa aggaattacc catgaagctc
     1441 tactacgctc ccggcgcctg ctccatggcg ccccacgtcg tgctgcgcga agccggctac
     1501 aagttcgatc tcgagaaggt cgacattccc aacaagaaga ccgcgggcgg agaggactac
     1561 tggaagatca acccgaaggg ctacgtgccg gcgctcaagc tcgacgacgg gcaggttctc
     1621 accgaggtgc aggtcatctg ccagtacctc gccgaccaca aggcggactc agggctcgcg
     1681 cccaaggcgg gcacgatgga gcgttaccgc ctcatggaaa tgctcaactt cacggcgagc
     1741 gaggtccaca agcagatcgg cgcgcttttc aaccccaaga tgaccccgga catgaaggaa
     1801 gtgcagctcg cctacgtcga gcggcggttg aacacgctcg agaagctgct cgcagggaag
     1861 cagtacgcga gcggcggcaa gttcagcatt gcagacgtct acctgttcac gatcctcaac
     1921 tggaccggct tgcacaagat cgatctcgcc aagtggccga acatcaaggc gttccaggcg
     1981 cgcatcgcgg cccggcccaa ggtgcaggaa acgatgaagg cggaaggcct cgtcaaataa
     2041 ccggacgcct tgtcagcggc tctcagggct tcagcgcctt gtagagaaac ggcaggctca
     2101 cgtccatgcg gtaatcgacg tcggagtggt cgtcgtcgaa ttcctcgtag cggtgccgga
     2161 ttcccgccgc ggcgaggcgc gccgacagga tgcgcgagcc gtaatggatg tgatactggt
     2221 cgcgccagcc gcagtcgatg tagatgccgc gcagcgactt caggttgtcg cggtatctcg
     2281 cgacgaggtt gatcgggtcg tgcccgcgcc acttcctcca gcgctcctcg atcgcctcgc
     2341 ccgactcgag gttgagcggc acgcggaagc cgagcggcgc cctcgggtcc ggatcgtacg
     2401 tggccgccat gcacaggttc atgatgcggt gg
//
LOCUS       DASPZD010000443         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_805152, whole genome shotgun sequence.
ACCESSION   DASPZD010000443 DASPZD010000000
VERSION     DASPZD010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805152"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VEM33_08580"
     CDS             <1..625
                     /locus_tag="VEM33_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HYS51923.1"
                     /translation="GVITLGAFSNLAKIGPRVITLWCRVLERLPTARLLIAGLGLASI
                     REEYLSRFANRGVAPERIDLRDFQSFTDYLALHGEVDVMLDTFPYTGGTTTCHALWMG
                     VPVVSLTGSSAPSRGGASLLGAIGLDELVVDTPDQYLNTVCDLASDPGRLSMLRAGMR
                     ARMSGSPLMDVSRFTYHLEQAYRSIWRRWCEDTKRKLENEGAGRTSQ"
     gene            597..2003
                     /locus_tag="VEM33_08585"
     CDS             597..2003
                     /locus_tag="VEM33_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYS51924.1"
                     /translation="MRALVERLSEIVGPAGLITAPQEVAPYATDWRKRYLGKAVAVVK
                     PASTAEVAGVVLVCADSRTAIVPQGGNTGLCGAATPDASGTQIILNLSRMNRIREVDA
                     RNNTMIVEAGCVLANLHRAAEEVGRLFPLSLAAEGSCEIGGNLSTNAGGTAVLRYGNA
                     RDLVLGLEVVLPSGEVWEGLRGLRKDNTGYDLKQLFIGAEGTLGVITAAVLKLFPLPK
                     SRATAVVALQSPEKALALLERALEACGERLTGFELFSDFCLALVLKHFGDAAAPFPRR
                     FPHYVLMELSDTQSGGGVRALAENVLEAALGEKNILDAAVAQSETQARDFWKLREFIS
                     EAQAHEGSNIKHDVSIPISRIADFISATDQELARAHAGVRLVTFGHVGDGNLHYNVSA
                     PEGVAPDVFVKNTAAINRLVHDSVARFGGSISAEHGLGQLKRAEIQRYKSALELELMR
                     KLKRALDPHGIMNPGKVL"
     gene            2200..>3056
                     /locus_tag="VEM33_08590"
     CDS             2200..>3056
                     /locus_tag="VEM33_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HYS51925.1"
                     /translation="MTEAEFQRLAAQGFNRVPLLAETFADLETPLSLYLKLANRSNTY
                     LLESVVGGERFGRYSFIGLAAPVRLRAVGSMVTVMHGDVIAEKSQTADPLAFVREFLS
                     RYRVAELPGLPRFCGGLVGYFGYDTVRWIEPRLAGGWLKPDPVGCPDMLLLVSEELAV
                     VDNLSGKLYLVVYADPAKARAWHDAQARLRELLGALRLPLSPPSETRDAETEVASNFD
                     AGTFRVAVDRAIRYVHDGDIMQVVLSQRLSRPFAGSSLSLYRALRSINPSPYMFYFDF
                     GDFQVVSASP"
BASE COUNT          516 a          937 c          992 g          611 t
ORIGIN      
        1 gggcgtgatt actctcggcg cattctccaa cctcgccaag atcggtcctc gggtcatcac
       61 tctgtggtgc cgggtgctgg agcgcctccc taccgctcga ttgctcatcg cgggcctggg
      121 tctggcctcg atacgcgagg agtatctctc ccgcttcgcg aaccggggag tggcgcccga
      181 gcgtatcgac ctgcgcgatt ttcagtcttt cacggattat ctcgcgttgc acggggaagt
      241 ggacgtgatg ctcgacacct ttccctacac tggcggcacg actacctgcc acgccttgtg
      301 gatgggcgtc cccgtcgtct ccctaacggg cagttcggcg ccttcgcgcg gcggggccag
      361 cctgctcggg gcgatcggac tggacgaact ggtggtcgat acgccggacc agtatctgaa
      421 cacggtgtgt gaccttgcaa gcgacccggg gcggctttcc atgctgcgtg cggggatgcg
      481 ggcacgaatg tcgggatcgc ctctcatgga tgtgtcgcgc tttacgtatc atctggaaca
      541 ggcctatcgc tcgatttggc gacgttggtg cgaagacacg aagaggaaat tggaaaatga
      601 gggcgctggt cgaacgtctc agtgagatcg tgggtcccgc gggcttgatc acggcgccgc
      661 aggaggtcgc gccctacgcg acagattggc ggaagcgcta cctgggcaag gcggtagccg
      721 tggtcaagcc tgcgtccacc gccgaggtgg ccggggtggt gcttgtgtgc gccgattcgc
      781 gtacggcgat cgtccctcaa gggggaaaca cgggcctttg cggcgccgcg accccggacg
      841 cgagcggcac gcagatcatc ctcaatcttt cgcgcatgaa ccggatccgc gaagtcgatg
      901 cgcgcaacaa caccatgatc gtggaagccg gttgtgtatt ggccaatttg cacagggcgg
      961 ccgaggaggt gggtcggctg tttcccttga gccttgctgc cgaaggcagt tgcgaaatcg
     1021 gcggcaatct ttccaccaat gcgggcggca cggcggtgct gcgctacggc aacgcccgcg
     1081 acctcgtgct gggactcgag gtcgtgctgc cctccggtga ggtgtgggag ggcctgcgcg
     1141 gcctgcgcaa ggacaacacc ggctatgacc tgaagcagct tttcatcggg gccgaaggga
     1201 cgctcggcgt tatcacagcg gcggtcttga aactctttcc cctgccgaag tctcgtgcga
     1261 ccgcagtggt ggcgctgcaa agtccggaaa aggcgctggc actgctggaa cgggctttgg
     1321 aggcctgcgg cgagaggctc actggattcg agctgttctc cgatttttgc ctcgcgctcg
     1381 tgctgaagca cttcggcgat gctgcggcgc cttttccgcg ccgcttccct cactatgtgc
     1441 tcatggaact ctcggacacg cagtcgggtg gaggcgtcag ggcattggcc gaaaacgtgc
     1501 tggaagcggc tctcggggag aaaaacattc tcgatgcagc ggtcgcgcag agtgaaacac
     1561 aggcccgcga tttctggaag ctgcgtgaat tcatttctga ggcgcaggcc cacgaggggt
     1621 cgaatatcaa gcacgatgtc tccatcccta tctcgcgcat cgccgatttc atatcggcga
     1681 cggaccagga actcgcccgc gctcatgcgg gcgtgcgcct ggtgactttc ggacacgtcg
     1741 gcgatggaaa ccttcactac aacgtatccg ctccggaggg ggttgctccc gatgtttttg
     1801 tcaagaatac ggcggcgatc aatcgcctgg tgcacgacag cgttgcgcgt ttcggcggct
     1861 cgatcagcgc cgagcacgga ctcggccagc tgaaacgcgc ggagatccag cgctacaagt
     1921 cggctctcga actggagctg atgcgcaagt tgaagcgcgc tctcgaccca cacggcatca
     1981 tgaatcccgg caaagtgctt tagcaagctg gacaagccgc gcaggggtgt tatagagtgg
     2041 gccccctgaa cgagacgcaa ggtaagtcaa ccgtgctctt cgaaaacgcc gggaaacggg
     2101 tgcaatcgcg cagctggcgc tggtggcgca gctagcccgc agtcgctcgc gcgccctgcg
     2161 cgcacccgca gtaccccgca acgttttcgt ggagcagtca tgaccgaagc agaattccag
     2221 cggcttgccg cacagggttt taaccgcgtt ccgctactcg cggagacctt cgccgatctc
     2281 gaaacgccgt tatcgcttta cctgaagctt gccaatcgct ccaacaccta tctgctcgag
     2341 tccgtggtgg gcggagagcg cttcgggcgc tattcgttca tcggactggc cgccccggta
     2401 aggctgcggg ccgtcgggag catggtgacg gtaatgcatg gagacgttat cgcggagaag
     2461 tcccagacgg ccgatccgct cgctttcgtg cgcgagtttc tttcgcgcta ccgcgtcgcg
     2521 gaactccccg gcttgccccg gttctgcggg ggcctggtcg gatacttcgg ctacgacacg
     2581 gtgcgctgga tcgagccgcg gctagccgga ggatggctca agcccgatcc ggtcggctgc
     2641 cccgacatgc tgttgttggt ctcggaagag ctggccgtgg tcgacaatct ttccggcaag
     2701 ctctatctcg tcgtctacgc ggaccccgcc aaggcgcgcg cctggcacga cgctcaggcg
     2761 cgcctgcgcg aactgctggg tgcgcttaga ctgcctttgt caccgccttc cgagacgcgc
     2821 gacgcggaga cggaggtcgc atcgaatttc gacgccggca ccttccgcgt cgcggtcgac
     2881 cgtgcaatcc ggtacgttca cgacggggac atcatgcagg tcgtgctgtc gcagcgtctg
     2941 tcgcgcccgt tcgcagggtc ttcgctgtcc ttgtatcggg ctctgcgctc gatcaacccg
     3001 tcaccgtaca tgttctattt cgatttcggc gacttccagg tggtcagcgc ctcacc
//
LOCUS       DASPZD010000444         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_651480, whole genome shotgun sequence.
ACCESSION   DASPZD010000444 DASPZD010000000
VERSION     DASPZD010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651480"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VEM33_08595"
     CDS             <1..493
                     /locus_tag="VEM33_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HYS51926.1"
                     /translation="ENHGKELSAALLAAGLLAAGPVWAQAPGGADPEGMGPMRGFERL
                     HQELNLNPQQEDLWKTAQAAQREAFRAMRAKGEETRAKLRVEIDKPGADLKQFAQLRD
                     QLGAQMRAQMETTRNQVREAWFAVYDTLDSVQKEKVRVAIRDGMDRMGRHHRGGPRAE
                     MHG"
     gene            complement(490..1440)
                     /locus_tag="VEM33_08600"
     CDS             complement(490..1440)
                     /locus_tag="VEM33_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYS51927.1"
                     /translation="MRRLLVLLAALAACAAHIPPPLPSGGPTALQFALIGDTPYSAAE
                     ATALDSMIEDINREDLAFVIHVGDITGGQGPCTDEWLEARKRQFEKLRHPFVIVPGDN
                     EWVDCHRSGFDPMERLGKFRELFESGDTSLANRSLRLERQSGGYAEYREHVRWVAAGV
                     LFVGVNVQGSNNNLGRTPAMDAEHRARMAAVLAWLEDSVRLAERSRLAGMLVFAQGDP
                     DFEGKMQRKGSNGFADFRNALRDLALRLGKPVLFVNGDTHLYKLDQPIADPATGRPLQ
                     NFTRVVVFGSPQTRWIRAGIDPASPRLFQVSPAPQFAPRP"
     gene            complement(1440..>2029)
                     /locus_tag="VEM33_08605"
     CDS             complement(1440..>2029)
                     /locus_tag="VEM33_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515863.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYS51928.1"
                     /translation="AADDLAGGRSTFMVEMTESANILHNATERSLVLMDEVGRGTSTF
                     DGLALAWAIARALLERNKALTLFATHYFEMTRLALEYKHAANVHLDAVEHKDTIVFLH
                     AVEEGPASQSYGLQVAQLAGVPKAVVRNARRYLQMLEEASLSRAGQSDLFAAGKSEPE
                     PPAEPDVLRDALAAANPDELSPREALELLYKLKRL"
BASE COUNT          330 a          666 c          717 g          316 t
ORIGIN      
        1 cgaaaatcat ggaaaggaat tgagcgccgc gctgttggca gccggcttgc tcgccgcagg
       61 cccggtctgg gcgcaggcac ccggcggagc cgaccccgaa ggaatgggac cgatgcgcgg
      121 tttcgagcgc ctgcaccagg agctgaatct gaatccgcag caggaagatc tgtggaagac
      181 ggctcaagcc gcgcagcgcg aggccttccg cgccatgcgc gccaaggggg aggagacacg
      241 cgccaagctg cgcgtcgaga tcgacaagcc gggcgccgac ttgaagcaat tcgcgcagct
      301 gcgcgaccag ctgggcgccc agatgcgcgc acagatggaa acgacgcgca accaggtgcg
      361 cgaggcctgg ttcgccgtgt acgacacgct cgattccgtt cagaaggaga aggtgagggt
      421 ggcgatccgc gacggcatgg accgcatggg acgccaccac cgcggcgggc cccgggccga
      481 aatgcacggc taaggccgcg gggcgaattg cggcgccggg ctcacctgga agagccgcgg
      541 cgacgccggg tctattcccg cccgaatcca gcgggtctgg ggggaaccga acaccaccac
      601 tcgggtgaaa ttctgcagcg gccggccggt cgccggatcg gcgatcggct gatcgagctt
      661 gtagagatgc gtgtcgccgt ttacgaacag gaccggcttt ccgagcctga gcgccagatc
      721 gcgcagagcg ttgcggaaat cggcgaaccc gttcgatccc ttgcgctgca tctttccctc
      781 gaagtccgga tcgccctgcg cgaagacgag cattccggcg aggcggcttc tttcggccag
      841 acggacgctg tcctccagcc acgcgagcac cgccgccatg cgcgcccggt gctcggcgtc
      901 catcgcgggc gtcctgccga ggttgttgtt gctgccctgc acgttgacgc cgacgaagag
      961 cacgcccgcc gcgacccagc gtacgtgctc gcgatattcc gcatagccgc ccgattgccg
     1021 ctcgagcctc aggctgcggt tggctaggct cgtgtccccg gattcgaaga gctcgcggaa
     1081 cttgcccagg cgttccatcg gatcgaagcc gctgcggtgg cagtccaccc attcgttgtc
     1141 gcccggaacg atcacgaacg ggtgcctgag tttctcgaac tggcgcttcc tcgcctcgag
     1201 ccactcgtcc gtgcaggggc cctggccgcc ggtgatgtcg ccgacgtgga tgacgaaggc
     1261 gaggtcctcg cggttgatgt cctcgatcat cgagtcgagc gccgtcgctt cggccgcgct
     1321 gtagggcgtg tcgccgatca gcgcgaactg gagcgcagtg ggtcctcccg agggcagcgg
     1381 cggcggaatg tgcgccgcgc aggcggcgag cgccgcgagc aggacgagca ggcggcgcat
     1441 cacaaccgtt tgagcttgta caggagctcc agcgcctcgc gaggggagag ctcgtcggga
     1501 ttggccgcgg cgagcgcgtc gcgcaggaca tcgggttcgg cgggcggctc gggctcggac
     1561 ttgccggcgg cgaagagatc cgactggcct gcacgcgaca ggctcgcctc ctccagcatc
     1621 tgcaggtacc tgcgcgcgtt gcgcacgacc gccttgggga cgccggcgag ctgagcgacc
     1681 tgcaggccgt agctctgcga agccgggcct tcctcgaccg cgtgcagaaa gacgatggtg
     1741 tccttgtgct cgaccgcgtc gaggtggacg ttcgccgcgt gcttgtattc gagcgcgaga
     1801 cgcgtcatct cgaagtagtg cgtggcaaac agcgtgagcg ccttgttgcg ctccaggagc
     1861 gcgcgcgcga tcgcccaggc gagcgcgagc ccgtcgaagg tcgaggtgcc gcgcccgacc
     1921 tcgtccatca gcaccaggct ccgctcggtc gcgttgtgca ggatgttggc cgattcggtc
     1981 atctcgacca tgaaggtcga gcgtccgccc gccaggtcgt cggccgcgc
//
LOCUS       DASPZD010000445         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_71262, whole genome shotgun sequence.
ACCESSION   DASPZD010000445 DASPZD010000000
VERSION     DASPZD010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71262"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VEM33_08610"
     CDS             complement(<1..558)
                     /locus_tag="VEM33_08610"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYS51929.1"
                     /translation="MNIAILGAGAWGTALAVSLCGKHRVSLWARDAALATAIGSSRRN
                     PRYLPEVSIPDSVAVTGDLLSAIAKCDAALIATPSSELREVLGRMRASRFEQPVIWAC
                     KGFEQASGRLPHQVAAEVLGKRTACGALSGPSFALEVAQGRPTALTLAAGDAAFARRW
                     ARELHHPTLRVYFSTDLAGVEISGAV"
     gene            complement(559..993)
                     /locus_tag="VEM33_08615"
     CDS             complement(559..993)
                     /locus_tag="VEM33_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HYS51930.1"
                     /translation="MRSLAVLALLAFALPVAAGEFRSISENGTPMYDAPSVRAKKLFV
                     ASRYYPVEVVINIDSWVKVRDQAGDLAWVEKKTLSDKRTVIVTAAVADARQAANDQAA
                     LVFQAQQGVALDVAEPPAGGWIRVRHADGQSGYVKINQVWGV"
     gene            complement(993..1448)
                     /gene="secB"
                     /locus_tag="VEM33_08620"
     CDS             complement(993..1448)
                     /gene="secB"
                     /locus_tag="VEM33_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HYS51931.1"
                     /translation="MSDVQQPTFGIEKIYVKDLSLEVPGGPQTFMQSEQPQLEVQITQ
                     QSQRVNELLFEVTLVITVSAKHGDKTLFLVEASQAGVFQIRNVPDADLAPVLGIVCPN
                     VLFPYARETVSDIVSRAGFPPVLLAPVNFEALYQQRTAAAAQPSIEIAH"
     gene            complement(1485..1742)
                     /gene="grxC"
                     /locus_tag="VEM33_08625"
     CDS             complement(1485..1742)
                     /gene="grxC"
                     /locus_tag="VEM33_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HYS51932.1"
                     /translation="MGKIRMYSTAVCPFCVRAERLLNSKGVTDIEKIRVDLDPRQREE
                     MINKTGRRTVPQIYIGDRHVGGFEELAALEHAGKLDSLLAA"
     gene            complement(1743..2168)
                     /locus_tag="VEM33_08630"
     CDS             complement(1743..2168)
                     /locus_tag="VEM33_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYS51933.1"
                     /translation="MADVDILKFVTDNKYVVAVAFVSGAMLVWPALRRGVAGATISTL
                     QATLLINQQNALVLDVREAAEYEKGHMLNARNIPLGELEARAGEIDKHKAKPVIVVCD
                     NGNRSGRAATALRKLGFEQVFRLGGGIGAWRQAGLPLEK"
     gene            complement(2170..>2489)
                     /locus_tag="VEM33_08635"
     CDS             complement(2170..>2489)
                     /locus_tag="VEM33_08635"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYS51934.1"
                     /translation="RDIFELIGRKEQIEKVARAMRAIAHPLRLKILCVLGEHELSVQD
                     IVEAVGTSQSNVSQHLAILREKRILSPRKNANHVYYSVSDQRTLHLIATMREVLCGGP
                     PRS"
BASE COUNT          433 a          824 c          770 g          462 t
ORIGIN      
        1 caccgccccg ctgatttcga cacccgcgag gtcggtgctg aaatacaccc tgagcgtggg
       61 gtggtgcagt tcgcgcgccc agcgcctcgc gaacgcggcg tcccctgccg cgagcgtcag
      121 tgccgtaggg cggccctgag ccacttccag cgcgaagctc gggccggaca aggccccgca
      181 cgcggtgcgt tttccgagca cctcggccgc gacctggtgt ggcaacctgc cgcttgcctg
      241 ctcgaatccc ttgcacgccc agatgacggg ctgctcgaac cgtgacgcgc gcatgcgccc
      301 cagcacctcg cgcaactcgg agctcggcgt ggcgatcagg gccgcgtcgc atttcgcaat
      361 tgcggagaga aggtcgccgg tcaccgcgac tgaatccggg atcgaaactt cggggagata
      421 acgtggattg cgccggctcg acccgatggc ggttgccagc gccgcatcgc gcgcccagag
      481 gctgacgcgg tgctttccgc agagcgagac cgccagcgcc gtaccccacg ctcctgcgcc
      541 gagaatcgcg atgttcagct agacgcccca gacctgattg attttgacgt agccgctctg
      601 gccgtcggca tgccgcaccc tgatccagcc gcccgccggc ggttccgcga catcgagggc
      661 cacgccctgc tgcgcctgga acaccagcgc cgcctggtcg ttcgcagcct gccttgcatc
      721 cgcgacggct gcggtcacga tcaccgtgcg cttgtccgag agcgtcttct tttccaccca
      781 ggcgaggtct cccgcctgat cgcggacctt gacccagctg tcgatgttga tcacgacttc
      841 gaccggatag taacggcttg cgacgaaaag tttcttggcg cgaaccgaag gcgcatcgta
      901 catcggcgtg ccgttttccg agatcgagcg aaactcgccc gccgcgacgg gcaacgcgaa
      961 cgcgagcagg gccaggaccg cgagactgcg catcagtggg ctatctcgat gctcggctgc
     1021 gccgcggcgg cggtgcgttg ctgatagagc gcctcgaaat tcacgggtgc gagcagcacc
     1081 gggggaaaac ccgcgcggct gacgatgtcg gaaacggtct cgcgcgcgta gggaaacaac
     1141 acgttcgggc agactatccc gagcaccggg gcgaggtcgg catcgggaac gttgcggatc
     1201 tggaacacgc ccgcctgcga ggcttccacc aggaacaggg tcttgtcgcc gtgctttgcg
     1261 ctcaccgtga tgaccagagt cacctcgaac aagagctcgt tcacgcgctg cgactgctgc
     1321 gtgatctgaa cttcgagctg cggttgctcg gactgcatga acgtctgcgg cccgcccggc
     1381 acttcgaggg acagatcctt cacgtagatt ttctcgatgc cgaacgtcgg ctgctgcaca
     1441 tcgctcatcg ggactccgat agaattgaaa gggcgcgcag gcggctaggc cgcgagcaac
     1501 gagtcgagct ttccggcatg ttcgagagcc gcgagttcct cgaatccgcc cacgtgccta
     1561 tcaccgatgt agatttgcgg gaccgtgcgc cggccggtct tgttgatcat ttcctcgcgc
     1621 tggcgcggat cgagatcgac gcggatcttc tcgatatcag tgacgccttt tgaattcagg
     1681 agcctctcgg cacgcacgca aaacgggcag acggcagtgg agtacatgcg aatcttcccc
     1741 atctactttt ccagtggcag ccccgcttgc cgccaggcgc cgatgccgcc gcccagcctg
     1801 aatacctgct cgaaacccag cttgcgcagc gcagtagcgg ccctgcccga gcggttgccg
     1861 ttgtcgcaca cgacgatcac cggcttagcc ttgtgcttgt cgatttcgcc ggcgcgcgcc
     1921 tcgagctccc cgagggggat gttgcgtgcg ttcagcatgt gccctttctc gtactccgcc
     1981 gcttcacgca catccagcac cagagcgttc tgctggttga tcagcagggt cgcctgaaga
     2041 gtcgaaatcg tggcgccggc caccccgcgg cgcagcgccg gccaaacgag catcgcgccg
     2101 cttacgaacg ccaccgcgac aacatatttg ttgtcggtga cgaatttcag gatgtcgacg
     2161 tccgccatgt cacgatctcg gcgggccgcc gcacagcact tcgcgcatgg tagcgataag
     2221 atgcagcgtg cgctggtcgc tcaccgaata atacacgtga ttggcgttct ttctgggact
     2281 gaggatgcgt ttttcacgga ggatcgccag atgttgcgag acgttgctct gcgaagtccc
     2341 gaccgcctcg acgatgtcct gtacgctcaa ctcatgctcg cccagaacgc acagaatctt
     2401 cagccgcaac ggatgggcga tggcccgcat tgcgcgggcg actttctcga tctgctcctt
     2461 gcggccaatt agttcgaaaa tgtcccggg
//
LOCUS       DASPZD010000446         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1017993, whole genome shotgun sequence.
ACCESSION   DASPZD010000446 DASPZD010000000
VERSION     DASPZD010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017993"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..522
                     /gene="rsfS"
                     /locus_tag="VEM33_08640"
     CDS             145..522
                     /gene="rsfS"
                     /locus_tag="VEM33_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HYS51935.1"
                     /translation="MTDVRKLQKAAIAALEDIKAREVEVFDVTHLTSMFDRVIIASAD
                     SARQLKALASHVQENAKAAGGRIYGVEGEDGGDWVLLDLGDIVVHIMQPAVRSHYNLE
                     ELWGQPKPRKPRARRKAVSARAR"
     gene            519..989
                     /gene="rlmH"
                     /locus_tag="VEM33_08645"
     CDS             519..989
                     /gene="rlmH"
                     /locus_tag="VEM33_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HYS51936.1"
                     /translation="MKLWVVAVGSRMPGWVNEAFEEYAKRMPRDLRIELVEVRPEPRS
                     GGKSTSQLLDAEAGRLSRAVPRGASRVALDERGREFTTAGLARWLAAQRQDGRDIAFL
                     IGGADGLAAAAKSGAELPLRLSAMTLPHGLARVLLAEQLYRASSILHNHPYHRQ"
     gene            986..1597
                     /locus_tag="VEM33_08650"
     CDS             986..1597
                     /locus_tag="VEM33_08650"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HYS51937.1"
                     /translation="MIKNPARTLIYLASRSPRRRELLKQIGVSFEVLVLRENPARGLD
                     VDESHLPGELPDDYVRRVCRAKADVGWDRVVQRRLRRFPVLAADTVVCVDEKILGKPA
                     DPADAARMLRLLSGREHRVLTAVSLRFETRTQLVVSESRVRFAELSGAELEAYVESGE
                     PTDKAGAYAIQGRAAAFVTELHGSYSGIMGLPLYETAQLIRKF"
     gene            1609..3069
                     /gene="rng"
                     /locus_tag="VEM33_08655"
     CDS             1609..3069
                     /gene="rng"
                     /locus_tag="VEM33_08655"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HYS51938.1"
                     /translation="MAEDILINCTPQETRVGVMQAGVPQELHIERAANRGLVGNIYVG
                     KVARVLPGMQSAFIEIGLERAAFLHVADIWEERQHPGAAPAKPIERILSEGQSRLVQV
                     IKDPIGTKGARLSTQISIAGRLVVYLPQDPHIGISQRIEDESERQLLREKLAQLLPAG
                     ETGGFIIRTVAETATEAELRGDIEYLKKLWHGIRSRALTAPAPTLLYQDLSLAQRVLR
                     DIAGESTGRIIIDSRENFQKLQEFATEYTPKVFEKLEHYTGERPLFDLHNVEDEIERA
                     LSRRVELKSGGYLILEQTEAMTTIDVNTGGYVGTRSFDDTIFKTNLEASQVIARQLRL
                     RNLGGMIIIDFIDMESAEHRSAVLAELNKALARDKTRITVNGFTGLGLVEMTRKRTRE
                     SLEHVLCEPCSTCGGRGVVRTAQTVCYEVLREILREARQFNAREFRILASQSVIDLFL
                     EEESQSLAMLGDFIGKPVSMQVETSYGQEQFDIILM"
BASE COUNT          556 a         1079 c         1084 g          464 t
ORIGIN      
        1 cggcactacg gcgctcgaca tttccgccag cgccatccgc gctcacttcg cccaggcgcg
       61 cagcccgcgc tatctgctgc cctcggccgt tctcgattac atcgaggcgc atggcgtgta
      121 cgctcagccg aacggcgccc cctgatgacg gacgtgcgca agctccagaa ggccgcgatc
      181 gcggcgctcg aggacatcaa ggcgcgcgag gtcgaggtct tcgacgtgac ccacctcacc
      241 tcgatgttcg accgcgtgat catcgcgagc gcagactccg cccgccagct gaaggccttg
      301 gcgagccacg tccaggaaaa tgcgaaagcg gcggggggcc gcatctacgg cgtggaaggg
      361 gaggacggcg gggactgggt gctgctcgac ctgggcgaca tcgtcgtgca catcatgcag
      421 ccggcggtgc gctcgcacta caatctcgag gaactgtggg gccagccgaa gccgcgcaag
      481 ccgcgcgcgc gtcgcaaggc ggtatcggcg cgcgccagat gaaactgtgg gtcgtcgccg
      541 tggggtcgcg catgcccggc tgggtgaacg aagccttcga ggagtacgca aaacgcatgc
      601 cgcgggactt gcgcatcgag ctcgtcgaag tccgccccga gccccgctcg ggcggcaaat
      661 cgacctcgca gctgctcgat gcggaagccg ggcgcctctc gcgcgccgtg ccgcggggag
      721 cctcccgcgt ggcgctcgac gaacgcgggc gtgaattcac cacggccggg cttgcgcgct
      781 ggctcgccgc gcagcgccag gacggccgcg acatcgcctt tctcatcggc ggcgcggacg
      841 ggctcgctgc ggcggcgaaa agcggcgcgg agctccccct gcgcctgtcc gcgatgacgc
      901 tgccgcacgg cctggcgcgg gtgctgctcg ccgagcagct ctaccgcgca agctcgatcc
      961 tgcacaatca tccctaccac cgccaatgat caagaacccc gcaagaaccc tgatctatct
     1021 cgcctcgcgc agcccgcggc ggcgcgagct cttgaagcag atcggcgtgt cgttcgaagt
     1081 gctggtgctg cgcgagaatc cggcgcgcgg cctcgacgtg gacgagtctc atctgccggg
     1141 cgagctgccc gacgattacg tccgccgcgt gtgccgcgcc aaagcggacg tgggctggga
     1201 ccgggtcgtg cagcgccggc tgcgaaggtt cccggtgctg gccgcggaca ccgtcgtctg
     1261 cgtcgacgag aaaatcctcg gcaagcccgc ggatcccgcc gacgcggcgc gcatgctgcg
     1321 gctgctttcc ggacgcgagc accgcgtgct caccgcggtc tcgctcaggt tcgagacgcg
     1381 cacccagctg gtcgtgagcg agagccgggt gcgtttcgcc gagctctcgg gggccgagct
     1441 cgaagcctac gtcgagagcg gtgagccgac cgacaaggcg ggcgcctacg cgatccaggg
     1501 ccgcgccgcg gccttcgtca ccgagctgca cggcagctac tcggggatca tggggctgcc
     1561 gctctacgag actgcgcagc tcatcaggaa gttctagatc gcgcatccat ggcggaggac
     1621 atcctgatca actgcacgcc gcaggaaacc cgcgtcggcg tgatgcaggc gggcgtgccg
     1681 caggagctgc acatcgagcg cgcggcgaac cggggcctcg tgggcaacat ctatgtcggg
     1741 aaagtcgcgc gcgtgctgcc cggcatgcag tcggcgttca tcgagatcgg gctggagcgc
     1801 gccgcgttcc tgcacgtcgc cgacatctgg gaggagcgcc agcacccagg cgccgcgccg
     1861 gcaaagccga tagagcgcat tctttcggag ggccagtcac ggctcgtgca ggtgatcaag
     1921 gatcccatcg ggaccaaggg cgcgcgcctc tcgacccaga tttcgatcgc cggacggctg
     1981 gtggtctatc tccctcagga cccccacatc ggcatctcgc agcggatcga ggacgagagc
     2041 gaacggcagc tgctgcgcga gaagctcgca cagctgctgc ccgccggcga gaccggggga
     2101 ttcatcatcc gcaccgtggc ggaaaccgcc accgaggccg agttgcgcgg ggacatcgag
     2161 tacctgaaaa aactctggca cggcatccgc agccgcgcgc tcacggctcc cgcgccgacg
     2221 ctgctctacc aggacttgtc gctcgcgcag agggtgctgc gcgatatcgc gggcgagagc
     2281 acgggccgga tcatcatcga ctcgcgcgaa aacttccaga agctccagga gttcgccacc
     2341 gagtacacgc ccaaggtgtt cgagaagctc gagcactaca ccggcgagcg gccgcttttc
     2401 gatcttcaca acgtcgagga cgagatcgag cgggcgctct cgcgcagggt ggaattgaaa
     2461 agcgggggct acctgatcct cgagcagacc gaagcgatga ccacgatcga cgtcaacacg
     2521 ggcggctacg tgggcacccg gagtttcgac gatacgatct tcaagaccaa tctcgaggcc
     2581 tcgcaggtga tcgcgcgcca gctgcgcctc cgaaacctcg gcggcatgat catcatcgac
     2641 ttcatcgaca tggagagcgc tgaacaccgc tccgcggtcc tcgccgagtt gaacaaggcg
     2701 ctcgcgcgcg acaagacccg gatcaccgtg aacggattca cggggctggg cctggtcgag
     2761 atgacgcgca agcgcacccg cgaatcgctc gagcacgtgc tgtgcgagcc ctgctcgacc
     2821 tgcggcgggc gcggcgtggt caggaccgcg cagacggtgt gttacgaagt cctgcgcgag
     2881 atcctgcgcg aggcgcgcca gttcaacgcg cgggagttcc gcatcctcgc ctcgcagtcg
     2941 gtgatcgacc tgttcctgga ggaggaatcg caatccctgg cgatgctcgg ggacttcatc
     3001 ggcaagccgg tgtcgatgca ggtcgagacg agctacgggc aggagcagtt cgacatcatc
     3061 ctcatgtagc ccatggaaaa ggaaaaagtc tcggcccggg aagccgcgct cgtcgcccag
     3121 gcgcggcgcg aggccgaagc gctcaagagg gcgccggcga ccgccccgcc tgccgctgcg
     3181 ccc
//
LOCUS       DASPZD010000447         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_530872, whole genome shotgun sequence.
ACCESSION   DASPZD010000447 DASPZD010000000
VERSION     DASPZD010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530872"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /gene="ybgC"
                     /locus_tag="VEM33_08660"
     CDS             <1..409
                     /gene="ybgC"
                     /locus_tag="VEM33_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HYS51939.1"
                     /translation="GDPYFSWPLRVYYQHTDAGGVVFHANYLSFMECARTELLQSQGF
                     DLGELARKDRILFVVHSAQIAYHKPALLNDLLTVTARVKHVGRARLQFDQAVMRREEV
                     LASAELSLACVSAKSYRPVAVPEAIRKKLEELR"
     gene            406..1122
                     /gene="tolQ"
                     /locus_tag="VEM33_08665"
     CDS             406..1122
                     /gene="tolQ"
                     /locus_tag="VEM33_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HYS51940.1"
                     /translation="MNTTQDLSILAMIASASLVVQLVILLLLVVSFLSWYFIFLKLFT
                     IHRAQSRTEHFERDFWSGSDLPALFQSAVNHRHSTGSLERIFEAGYREFQKLKGQRGV
                     ADPSVMVDGARRAMRATYQREMDRLEASLSFLASVGSVSPYVGLLGTVWGIMHAFRGL
                     ANMAQATLAAVAPGIAEALVATAIGLFAAIPAVVAYNRFSHNVDRLAVRFESFMEEFS
                     NILQRQAQARLASAHEPTRT"
     gene            1127..1540
                     /gene="tolR"
                     /locus_tag="VEM33_08670"
     CDS             1127..1540
                     /gene="tolR"
                     /locus_tag="VEM33_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HYS51941.1"
                     /translation="MRARRLMNEINIVPYVDVMLVLLVIFMVTAPLVNPGVIDLPSVG
                     KSSQPPASPLEVIVRRDQSLLLRERDKGRADERAVTPAQLVAALRERQKKSPDVPVVI
                     SADKDVRYEAVLKVMDELQRQNIRRVGLLVKPATP"
     gene            1537..2226
                     /locus_tag="VEM33_08675"
     CDS             1537..2226
                     /locus_tag="VEM33_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HYS51942.1"
                     /translation="MSYAIPADEPGGVAPAALSLLVHGVLFAVLVFGLRWQSKHPDAV
                     VAELWSELPAIEPPEPNPEIKPQPKPEPKIEQKTEKPDIALEREKKLPKKKEEPPLKF
                     DTTQRIREQLAQEQKALQDRERKDVLKQFASPPTAGLPDAGYIDKIRTKIKTHIVPPS
                     EIKGNPEVVFNIVQLPTGEVLTVRLVRTSGNALLDSAVERAILKSSPLPKPDRPEQWL
                     REFNISFRPLE"
     gene            2223..>2552
                     /locus_tag="VEM33_08680"
     CDS             2223..>2552
                     /locus_tag="VEM33_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HYS51943.1"
                     /translation="MSSRTFAPLIAAILLLAGPARAQLTIEIIGGGANQIPIAVLPFA
                     GEPVLPQSITEIIEQDLSRSGRFRAIFVGGVNPPPTEIAQVNFPEWKSRLADAIVIGQ
                     AQRLPDGK"
BASE COUNT          468 a          841 c          827 g          416 t
ORIGIN      
        1 cggcgaccct tacttctcct ggccgcttcg cgtctactac cagcataccg acgcaggcgg
       61 cgtggtgttc cacgccaact acctctcgtt catggaatgc gcgcgcaccg agctgctgca
      121 gtcgcaaggg ttcgacctgg gagagctcgc gcgcaaggac cgcatcctct ttgtggtgca
      181 ttcggcgcaa atcgcctacc ataagcccgc gctgctgaat gatctgctga cggtgaccgc
      241 ccgggtgaag catgtgggca gggcgcggct ccagttcgat caggcggtca tgcgcaggga
      301 ggaggtactg gcgagcgccg agctgagcct cgcgtgcgtc agcgcgaaga gctacaggcc
      361 cgtcgcggtg cccgaggcga ttcgaaaaaa actagaggaa ttgcgatgaa cacgacccag
      421 gacctctcga tcctagcgat gatcgcgagc gcgagcttgg tcgttcagct cgtcatactg
      481 ctgctcctgg tcgtgtcctt cctgtcctgg tacttcatct ttctcaagct cttcaccata
      541 caccgcgccc agagcaggac cgagcacttc gagcgcgact tctggagcgg ctcggacctg
      601 cccgcgctgt tccagagcgc ggtgaaccac cggcattcga ccgggagcct cgagcgcatt
      661 ttcgaggccg ggtaccgcga gttccagaaa ctgaaaggcc agcgcggtgt ggccgatccg
      721 agcgttatgg tggacggggc cagacgcgcg atgcgagcga cgtatcagcg cgagatggac
      781 cgcctcgaag ccagcctctc cttccttgcc tcggtgggct cggtgagccc ctacgtcggg
      841 ctgctcggca cggtatgggg catcatgcac gcgttccgcg gcctcgccaa catggcgcag
      901 gcgacccttg cagccgtcgc cccggggatc gcggaggccc tggtcgccac ggcgatcggc
      961 ctgttcgcgg ccatacccgc agtggtcgcc tacaaccgct tctcccacaa cgtcgaccgc
     1021 ctcgccgtgc gcttcgagag cttcatggaa gagttctcca acatcctgca gcgtcaggcc
     1081 caggcgaggc tcgcgagcgc gcacgaacca acgcggacct gaggcgatgc gcgcccgccg
     1141 tctcatgaac gagatcaaca tcgtcccgta cgtggacgtg atgctggtgc tgctggtgat
     1201 tttcatggtc accgcgccgc tcgtgaaccc gggcgtgatc gatctgccga gcgtgggcaa
     1261 gtcctcgcag ccgccggcct cgccgctcga ggtgatcgtg cggcgcgatc agtccttgct
     1321 gctgcgcgag cgcgacaaag gcagggcgga cgaacgcgcg gtgacgccgg cgcagctcgt
     1381 ggcggcattg cgcgagaggc agaagaagag ccccgatgtg ccggtggtga tctcggcgga
     1441 caaggacgtg cgctacgagg cggtcctgaa agtgatggac gagctgcagc ggcagaacat
     1501 ccgtcgcgtc gggctgctcg ttaagcccgc caccccgtga gctacgcgat ccccgcggac
     1561 gaaccgggcg gcgtcgcacc cgcggcgctg tcgctgctcg tgcacggcgt gctctttgcg
     1621 gtgctggttt tcgggctgcg ctggcagtcg aagcacccgg atgctgtcgt ggccgaacta
     1681 tggagcgagt tgccggcgat cgagccaccc gagcccaacc ccgagatcaa gccgcagccg
     1741 aagccggagc ccaagatcga gcagaagacg gagaagcccg acatcgcgct cgagcgggaa
     1801 aagaagctcc cgaagaaaaa ggaggagccg ccgctcaagt tcgataccac ccagcgcatc
     1861 cgcgagcagc tcgcgcagga gcaaaaggcg ctgcaggacc gcgagcgcaa ggacgtcctc
     1921 aagcagttcg catcgccacc tacggcaggg ttgccggacg cgggctacat cgacaagatc
     1981 cggaccaaga tcaagacgca catcgtcccg ccgtccgaga tcaagggaaa cccggaggtg
     2041 gtcttcaaca tcgtgcagct gcccacgggc gaggtgctca cggtgcggct cgtcagaacg
     2101 agcggcaatg cgctcctgga ctccgccgtc gaacgggcta ttctgaagag ctccccgctg
     2161 ccgaagcccg atcgacccga gcaatggttg cgcgagttca atatcagctt tagacccttg
     2221 gaatgagctc gcggacgttt gccccgctca ttgcggcgat cctgctcctc gcggggccag
     2281 cccgggccca gctcaccatc gagatcatag ggggcggcgc caaccagatt cccatcgcgg
     2341 tgcttccgtt cgcgggggag ccggtgctgc cgcagagcat cacggaaatc atcgagcagg
     2401 acctctcgcg aagcggccgc tttcgcgcga tattcgtcgg cggggtcaat ccgcctccca
     2461 ccgagatcgc ccaggtcaat ttccccgaat ggaagagccg gctcgccgac gccatcgtca
     2521 tcgggcaggc gcagcgcctt cccgacggaa ag
//
LOCUS       DASPZD010000448         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_343817, whole genome shotgun sequence.
ACCESSION   DASPZD010000448 DASPZD010000000
VERSION     DASPZD010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343817"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1019)
                     /locus_tag="VEM33_08685"
     CDS             complement(93..1019)
                     /locus_tag="VEM33_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51944.1"
                     /translation="MIERRDEELSHIYREAEGPRPPQRVDDNILAASQRVAGTVRRPA
                     VFSFSRRWGAPVAIAATVLVTSTVALMVHERRSGVDTAAPGVPRAGRAAKVSPAAPVR
                     TDQPAKSPSTEPRAEPSEKSPPAEPRSAAPVTPSPKAAAPLDRAKPATVETALSGERR
                     ATGQPARLPAIPPGVEILRKTDEPKPAPAAAAPAAASERARATQQSPVPDALRTFDVQ
                     PLIRPAQAPASAPAPGANTLRESASTLQGAGAVGALTSSVAVSDAKERTPEKWLEDIR
                     KLKTEGKAIEAERDLVEFKKRYPDYILPEDLR"
     gene            complement(1016..1666)
                     /locus_tag="VEM33_08690"
     CDS             complement(1016..1666)
                     /locus_tag="VEM33_08690"
                     /inference="COORDINATES: protein motif:HMM:NF009166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYS51945.1"
                     /translation="MSDLASVLPFPPRALPVRHRRAAEALHLRSMRIEASDEELMLRY
                     GRGDARAFELLYRRYRLPLYRFLLRQVGNAATAEELFQDLWMRVVNSRGRYEARAKFA
                     SWVYAIAHNRLMDFYRGNGRASFLSHEESESVLEDLPAAEISADLRLDRKRAAERLVA
                     ALGELPDAQREAFLLQQEGEMSIEEIAAATGVGPETAKSRLRYAVAKLRASLGKES"
     gene            complement(1725..>2307)
                     /locus_tag="VEM33_08695"
     CDS             complement(1725..>2307)
                     /locus_tag="VEM33_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51946.1"
                     /translation="SGFAATANPPREKVGNLYLSKFDWWREPFMASFSAGSFDFVLLA
                     AHAQWGTPAGRLVELQSIAEWIDLKRKEKTCEDKDLIIVGDFNIETPEQLAALTGKGL
                     QMPAALKGKSFGTNLAQDKRYDQILHYPGYPENFSNQAGVVDFYAGSSKELFPDLEKT
                     AFTFQMSDHLPLWMQINTDIEGQKLDEIIRAGK"
BASE COUNT          360 a          757 c          785 g          405 t
ORIGIN      
        1 cgggaagcgg cgcgccgcgc ggcgctgcgg cagtgtacgg agaataccct gacgaaagct
       61 tgaacgatcc ggacgattcg gactccggcc ggctagcgca ggtcctccgg caatatgtaa
      121 tcgggatagc gtttcttgaa ctcgaccaga tcgcgctcgg cttcgatcgc cttgccctcg
      181 gtcttgagct tgcgaatatc ctcgagccat ttttccggcg tgcgctcctt ggcgtcggag
      241 acggcgacag agctggtcag agcaccgaca gcgccggctc cctggagcgt cgatgcggat
      301 tcccgaagag tgttcgcgcc cggggccggg gcagacgcgg gcgcctgcgc cggccggatc
      361 agcggttgaa cgtcgaaggt gcgcagggca tccggcacgg gcgattgctg ggtcgcgcgg
      421 gctcgctcgc tcgccgctgc cggagccgcg gcggcgggcg ccggctttgg ctcgtcagtc
      481 ttgcgaagaa tttccacccc aggcgggatt gccggcaggc gcgcgggttg ccccgttgcg
      541 cggcgttcgc ccgagagggc ggtttcgacc gtagccggtt tcgctcgatc aagcggggcc
      601 gcggctttcg ggctcggcgt gaccggcgcg gcgcttcgcg gctccgcagg cggagatttc
      661 tccgacggct cggcgcgcgg ttccgtggac ggggatttcg ccggctgatc ggttcgcaca
      721 ggggctgcgg gagaaacttt cgccgcccgt ccggcgcgcg gcacgccggg cgccgcggtg
      781 tcgacgccgg accgccgctc gtgcaccatg agcgcgaccg tggaggtcac cagcacggtc
      841 gctgcgatcg cgaccggcgc gccccagcgg cgcgagaacg agaacaccgc gggccgccgc
      901 accgtgccgg cgacgcgctg cgacgccgcc aggatattgt cgtcgacccg ctgcgggggg
      961 cgcgggccct ccgcttcacg gtagatgtgg gaaagctcct cgtcgcgccg ctcgatcatg
     1021 attccttccc caggctcgca cgcagcttcg ccacggcata gcgcaggcgg ctctttgcgg
     1081 tctcggggcc gacgccggtc gccgcggcaa tttcctcgat cgacatttcg ccctcttgct
     1141 gcagtaaaaa ggcctcgcgc tgcgcatcgg gaagctcgcc gagcgcggcg accagacgct
     1201 ccgccgcgcg cttccggtcc aggcgcaggt ccgctgaaat ctccgcggcg ggaaggtcct
     1261 cgagaacgga ttcgctttcc tcgtggctca ggaacgacgc ccggccgttg cctcgataga
     1321 aatccatcag gcgattgtgc gcgatggcgt atacccagga ggcgaatttc gcccgggcct
     1381 catagcgccc gcgggaattg acgacgcgca tccagaggtc ctggaacaat tcttcggctg
     1441 tggccgcatt gccgacctgt cgcagcagga accggtacaa ggggagacga tagcggcggt
     1501 agagcagctc gaaggcgcgg gcatcccccc tgccgtagcg cagcatcagc tcctcgtcgc
     1561 tcgcctcgat cctcatgctg cgcaagtgta gcgcctccgc agccctgcgg tgccgcacgg
     1621 ggagcgcgcg aggcgggaag ggcaggaccg aagcgagatc cgacatcctt gagttaacgc
     1681 cgtccacgcc cgaacagggt taagcaaatc ggctcccggc cgcatcactt tccggcccgg
     1741 atgatctcgt cgagcttctg accctctatg tcggtgttga tctgcatcca cagcggaagg
     1801 tgatcggaca tctgaaaggt gaacgcggtt ttctccagat cggggaacag ctctttcgag
     1861 ctcccggcgt agaaatcgac cacgcccgcc tggttcgaga aattctccgg ataccccggg
     1921 tagtgcagga tctgatcgta gcgcttgtcc tgggcgaggt tggtgccgaa ggacttgccc
     1981 ttgagggcgg cgggcatctg aagccctttc ccggtcaggg ccgcgagctg ctcgggagtt
     2041 tcgatgttga agtccccgac gatgatgaga tccttgtcct cgcaggtctt ctcctttcgc
     2101 ttcaggtcga tccattcggc gatggactgc agctccacca ggcgccccgc gggtgtcccc
     2161 cactgcgcgt gcgccgcgag aaggacgaaa tcgaaacttc cggcactgaa cgacgccata
     2221 aagggctcgc gccaccagtc gaacttggac aggtacaggt ttccgacttt ctcgcgcggc
     2281 gggttggccg tcgcggcgaa tccgctg
//
LOCUS       DASPZD010000449         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1615321, whole genome shotgun sequence.
ACCESSION   DASPZD010000449 DASPZD010000000
VERSION     DASPZD010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615321"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="VEM33_08700"
     CDS             <1..1665
                     /locus_tag="VEM33_08700"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HYS51947.1"
                     /translation="PLPIKAYGRRVEDLLAEFTQAGRGKVTVEKLDPQPDSVAEDSAT
                     LEGVEAQVTPSGDKFYLGASISYADQKLALPALAPDREPLLEYDLVRAIARAAATAKP
                     VIGVMSALPVFGMPPSPMTGGQPMEPQVFIGELRRDYTVKRVGMDVEKIDDDIKVLLV
                     IHPRGIGERAQYALDQFVLRGGKLIAFLDPSAYFDQLGQMGGMGGGGTPSSLDKLLKA
                     WGLAFDSGKVVLDMRYLTGAGARTLPTLLSLNDNAFDPHDVATARLGSLLMPFAGAFT
                     GKPAEGLSEAVLMKSSTYSQLVDSFAATAQGEAAIRAFKPSGTEYPLAVRLSGKFKTA
                     FPEGQPKAEERGEKKAATGAKASDAKPEAAQLKESAGENAVVLVGDSDFINDGAAVQI
                     QEIFGQRIVIPRNGNIAFAQALVDQFAGDPALIKLRSRASAARPFTVIRDMEAKAQQA
                     YLGKIKELEDNLGQTQEKLNALQKGKGQGAGAILSAEQQTELENFRRKAAETRLALKE
                     VRRELRADSEALQFWTKVANIALMPILVALAGIVLAVLRRRRTPAL"
     gene            1662..>2715
                     /locus_tag="VEM33_08705"
     CDS             1662..>2715
                     /locus_tag="VEM33_08705"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HYS51948.1"
                     /translation="MNRRQFFIVLAALAVLAALGAAVVLSERSAWTSADSRAGQKAMA
                     GLRLSEVAEIAIRDSSGGLHLDRGESGWSVRERAGFAADTDRIAGLLVKLAELKVVQS
                     EPLPEGQRARLELLEPKDRAQGAGTAIELKDAKGGSLGRLLLGKKIVKSPPVASLSRG
                     EAEATGRYLTLGGDAGTMLAVGEPLTEVGSKPDQWLVKDLVRADGVKTVSSSKDGRSR
                     WSVTRETESADWKFAGSGEKPDLQKATDLASSLGWVNLVDVVADPAKSDTGLDHAIVI
                     KAETFDGLIYTLRIGNKVGDNYYVSLAVAGEPRKTRAPAKGEKAEDKAKNDKEFEERR
                     KKLVEKVDREKKLERWT"
BASE COUNT          496 a          846 c          971 g          402 t
ORIGIN      
        1 cctctgccca tcaaggcata cgggcgcagg gtcgaggatc tgctcgcgga attcacgcag
       61 gccggccgcg gcaaggtcac ggtcgagaag ctcgatccgc agccggactc cgttgccgag
      121 gactcggcga ccctggaagg cgtcgaagcg caagtcacgc cctcgggcga caagttctac
      181 ctcggcgcct cgatcagcta tgccgaccag aagctcgcct tgccggcgct tgcgcccgat
      241 cgcgagccgc tgctcgagta cgatctggtg cgcgcgatcg cgcgcgccgc cgcgacggcg
      301 aagccggtga tcggagtgat gagcgcattg ccggtgttcg gcatgccgcc cagtcccatg
      361 accggcggcc agccgatgga gccgcaggtg ttcatcggcg agctccggcg cgactacacg
      421 gtgaagcgcg tcggcatgga tgtcgaaaaa atcgacgacg acatcaaggt gctgctggtg
      481 atccatccgc gcggcatcgg cgagcgggcg cagtacgcgc tcgaccagtt cgtgctgcgc
      541 ggcgggaagc tcatcgcttt cctcgacccg agcgcctatt tcgatcagct gggccagatg
      601 gggggcatgg gcggcggggg cacgccgtcc tcgctcgaca agctgctcaa ggcctggggc
      661 ctcgccttcg actcggggaa ggtcgtgctc gacatgcgct accttacggg cgcgggagcg
      721 cgaacgctgc cgacgctgct ctcgctcaac gacaatgctt tcgatccgca cgacgtcgcg
      781 acggcgcggc tggggtcgct gcttatgcct tttgcgggag cgttcaccgg caagcccgcc
      841 gaggggctga gcgaggcggt cctcatgaag agctccacgt attcccagct cgtcgattcg
      901 ttcgcggcga cggcgcaggg cgaggcggcg atccgggcgt tcaagccttc gggcaccgaa
      961 tatcctctgg ccgtgcggct gagcggcaag ttcaagaccg cgttccccga gggccagccg
     1021 aaggcggagg aaaggggcga aaagaaggcg gcaacggggg caaaggcgtc cgacgcgaaa
     1081 ccggaagccg cgcagctcaa ggaatcggcg ggcgagaacg ccgtcgtgct cgtcggggac
     1141 agcgatttca tcaacgacgg cgcggcggtg cagatccagg agatcttcgg gcagcggatc
     1201 gtgatcccgc gcaacggcaa catcgctttc gctcaagccc tggtggatca gtttgccggg
     1261 gatccggccc ttatcaagct gcgcagccgc gcgagcgccg cgcggccgtt caccgtgatc
     1321 cgcgacatgg aggcgaaggc gcagcaggcc tacctcggca agatcaagga gctggaggac
     1381 aacctcgggc agacgcagga aaagctgaac gcgctgcaaa aaggcaaggg gcagggcgcg
     1441 ggggcgatcc tctcggccga gcagcagacg gagctagaga acttccgcag gaaagccgcg
     1501 gagacgcgcc tcgcgctgaa agaggtccgc cgcgagctgc gcgccgacag cgaagcgctc
     1561 cagttctgga ccaaggtggc gaatatcgcg ttgatgccga tcctcgtggc gctcgccggc
     1621 atcgtgctcg cggtcctgcg ccggcgcagg acacccgccc tgtgaaccgc aggcagttct
     1681 ttatcgtgct tgcagcgttg gcggtcctcg ccgcgctggg cgccgccgtc gtcttgtccg
     1741 agcgctcggc ctggacgagc gccgattccc gcgccgggca gaaggcgatg gcgggcctgc
     1801 gcttgagcga ggtcgccgag atcgcgatcc gggattcctc gggcgggctg caccttgacc
     1861 gcggcgagtc cggctggagc gtgcgcgagc gcgccggttt cgcagccgac acggatcgta
     1921 tcgccggcct tctggtgaag ctcgccgaat tgaaggtcgt gcagagcgag cccctgcccg
     1981 agggccagcg cgctcgcctg gagctcctcg agccgaagga cagggcgcag ggtgcgggga
     2041 cggcgatcga gctgaaggac gccaagggcg gctcgctcgg acggctgctt ctcggaaaga
     2101 aaatcgtgaa gagcccgccg gtcgcctcgc tttcgcgggg cgaggccgag gcgacgggcc
     2161 gttacctcac gctgggcggc gatgccggca cgatgctcgc ggtcggcgag ccgctgaccg
     2221 aagtcggctc caagcccgat caatggctgg tgaaggactt ggtgcgggcc gacggcgtga
     2281 aaacggtttc gtcgtcgaag gacggaaggt cgcgctggag cgtgacgcgc gaaaccgaat
     2341 ccgccgattg gaaatttgcg ggctccgggg aaaaacccga tctgcagaag gcgaccgatc
     2401 tcgcgagctc gctcggctgg gtgaatctcg tcgatgtggt cgccgatccc gccaagagcg
     2461 acaccgggct cgatcatgcg atcgtcatca aggccgagac cttcgacggg cttatctata
     2521 ccctgcggat cggcaacaag gtgggcgaca attattacgt cagccttgcc gtcgccggcg
     2581 aaccgcgcaa gacgcgtgcg cccgcgaagg gcgagaaagc ggaagacaag gcgaagaacg
     2641 acaaggagtt cgaggaacga cgcaagaagc tcgtcgagaa ggtcgatcgg gagaaaaagc
     2701 tcgagcgctg gactt
//
LOCUS       DASPZD010000450         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1012848, whole genome shotgun sequence.
ACCESSION   DASPZD010000450 DASPZD010000000
VERSION     DASPZD010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012848"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..457
                     /gene="rpsK"
                     /locus_tag="VEM33_08710"
     CDS             50..457
                     /gene="rpsK"
                     /locus_tag="VEM33_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYS51949.1"
                     /translation="MAKPPAAPTAAARVRKKVKKNVAEGIAHIHASFNNTIVTITDRQ
                     GNALAWATSGAAGFKGSRKSTPFAAQVAAEQAGRAAQECGVKNLEVRIKGPGPGRESA
                     VRALNALGLKISSIADVTPVPHNGCRPPKKRRI"
     gene            468..1097
                     /gene="rpsD"
                     /locus_tag="VEM33_08715"
     CDS             468..1097
                     /gene="rpsD"
                     /locus_tag="VEM33_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYS51950.1"
                     /translation="MARDLDAKCRQCRREGEKLFLKGEKCFTDKCAIERRSYAPGQHG
                     QKSGGRMSDFGKQLREKQKLRRIYGLLERQFRKTYAEASRSKGVTGERLLQLLESRLD
                     TVSYRMGFGASRTEARQIVRHNAILVNGKRVNMPSYQVRPGDTVEVVAKAKEQLRIKG
                     AVAAAETRGFPEWIEMNAKEMKGRFKALPARSDLPSTINESLVVELYSK"
     gene            1148..2134
                     /gene="rpoA"
                     /locus_tag="VEM33_08720"
     CDS             1148..2134
                     /gene="rpoA"
                     /locus_tag="VEM33_08720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HYS51951.1"
                     /translation="MQTSGFLKPRIIDVQGIAPNHAKVVMEPFERGYGHTLGNALRRV
                     LLSSMPGFAATEVQIAGVLHEYSTIEGVQEDVVDILLNLKGVVLKLHNREDALLNLKK
                     KGEGPVTAGDIEATHDVEIINPEHVIAHLSPGGKLEMQIKVENGRGYIPGNLRVVPEE
                     SKGIGKIILDASFSPVRRVSYSVESARVEQRTDLDKLIIDIETNGVIEPEEAIRYAAR
                     ILVDQLSVFAALEGTAPAIEEKKAPQVDPILLRPVDDLELTVRSANCLKAENIYYIGD
                     LIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLETARTA"
     gene            2150..2548
                     /gene="rplQ"
                     /locus_tag="VEM33_08725"
     CDS             2150..2548
                     /gene="rplQ"
                     /locus_tag="VEM33_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HYS51952.1"
                     /translation="MRHRHGLRKLNRTSSHRLAMLRNMTVSLLMHEVIQTTLPKAKEL
                     RRVVEPMITLGKRATLANRRLAYNRLRDRGIVTKLFAELGPRYAKRNGGYLRILKYGF
                     RKGDNAPMALVELLDRPAPTDEPKSAEAKE"
BASE COUNT          571 a          817 c          796 g          397 t
ORIGIN      
        1 aagattaccg ccgccgcccc gaaggccggc tgagagcgag cgaggaccca tggcaaaacc
       61 gcctgccgca ccgaccgccg cagcacgcgt tcgcaagaag gtgaagaaaa acgtcgcgga
      121 gggcatcgcc cacatccacg cctcgttcaa caacaccatc gtcaccatta ccgatcgaca
      181 aggcaatgcc ctggcatggg cgacttccgg cgccgcgggc ttcaagggct cgcgcaaatc
      241 gacaccgttc gccgcccagg ttgcggccga gcaggccggc cgcgcggcac aggagtgcgg
      301 agtgaagaat ctcgaggtgc gcatcaaagg ccccggcccc ggacgggaat ccgccgttcg
      361 cgccctgaac gcgctgggcc tcaagatcag cagcatcgcg gacgtgacgc ccgtgcccca
      421 caacggttgc aggccgccga aaaagcggcg catatagagg aatacgcatg gcccgagatc
      481 tggatgccaa gtgccggcag tgccgccgcg agggagagaa gctcttcctc aagggcgaaa
      541 agtgtttcac ggacaaatgc gcgatcgaac gccgcagcta tgcgccgggg cagcacggcc
      601 agaaaagcgg cggccgcatg tccgatttcg gcaagcagct gcgcgagaag cagaagctgc
      661 gccgcatata cgggctgctc gaacgccagt tccgcaagac ctatgcggag gcgtcgcgca
      721 gcaagggcgt gacgggcgag cgcctcctgc agctgctgga atcgcgtctg gacaccgtgt
      781 cgtatcgcat ggggttcggt gcgagccgca ccgaggcgcg ccagatcgtg cgccacaacg
      841 ccatcctcgt aaacggcaaa cgcgtcaaca tgccgtccta ccaggtgcgg ccgggcgaca
      901 ccgtcgaagt cgtggcgaag gcgaaggagc aacttcgcat caaaggcgcg gtcgctgctg
      961 cagagacgcg cggcttcccg gaatggatcg aaatgaatgc caaggaaatg aagggcaggt
     1021 tcaaagcgct cccggcgcgt tcggatctgc cttcgacgat caacgaaagc ctggtggtcg
     1081 agctgtactc gaaataacgc tgcactcgaa gtatccggcg caagctaatt cgtatcgagg
     1141 gtcttacatg caaaccagtg gatttctgaa accgcgcatc atcgacgtgc agggcatcgc
     1201 ccccaaccac gccaaagtcg tgatggagcc gttcgagcgc gggtatggcc atacgctcgg
     1261 caatgccctg cgccgcgtcc tgctttcgtc gatgcccgga ttcgccgcga ccgaagtgca
     1321 gatcgcggga gtgcttcacg aatactcgac gatcgagggc gtccaggagg acgtcgtcga
     1381 tatcctcctc aacttgaagg gcgtggtgct caagctccac aaccgagaag atgcgctgct
     1441 caatctgaag aagaagggcg aaggcccggt gaccgcaggc gacatcgagg ccactcacga
     1501 cgtcgagatc atcaaccccg agcatgtgat cgcgcatctc tcccccggcg gcaagctcga
     1561 gatgcagatc aaggtcgaga acggccgcgg atacatcccc gggaatctcc gcgtcgttcc
     1621 cgaggaatcc aagggcatcg gcaagatcat actggacgcc tccttctcgc ccgtcagacg
     1681 cgtgtcctat tcggtcgaat cggcccgcgt ggagcaacgt accgatttgg acaagctcat
     1741 catcgacatc gagacgaacg gggtgatcga accggaggag gcgatccgtt acgccgccag
     1801 gatcctggtc gatcaactct ctgtcttcgc ggcgctcgaa ggcacggcgc cggcgatcga
     1861 ggaaaagaag gccccgcagg tcgatcccat tctgctgagg cccgtcgacg atctcgagct
     1921 cacggtgcgt tcggcaaact gcctgaaggc ggaaaacatc tattacatcg gcgacctgat
     1981 ccagcgcacc gagaccgagc ttctcaagac gcccaatctc ggtcgcaagt cgctcaacga
     2041 gatcaaggaa gtgctcgctt cgcgtgggct gacgctcggc atgaagctcg agaactggcc
     2101 gcctgccggg ctggaaaccg cgcggaccgc ctgaatcggg gcaaaggata tgcgccaccg
     2161 ccacggactc aggaagctca accgtaccag cagccatcgc ctggcgatgc tgcgaaacat
     2221 gaccgtctcg cttttgatgc acgaggtcat tcagaccact ctccccaagg caaaggagct
     2281 gcgccgcgtg gtcgaaccca tgatcacgct cggcaagcgc gccacgctcg ccaaccgtcg
     2341 cctcgcctac aaccgcctgc gcgaccgcgg catcgtgacg aagctcttcg ctgagctcgg
     2401 gccgcgctat gccaagcgca acggcgggta cttgcgcatc ctgaagtacg gttttaggaa
     2461 gggagacaac gctcccatgg ctctggtgga gttgctggat cgtcctgcgc ctacggacga
     2521 acccaagtcg gcggaggcca aagagtagtt gttccgcatg accaaaagaa aaggccggat
     2581 c
//
LOCUS       DASPZD010000451         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_50271, whole genome shotgun sequence.
ACCESSION   DASPZD010000451 DASPZD010000000
VERSION     DASPZD010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50271"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VEM33_08730"
     CDS             <1..257
                     /locus_tag="VEM33_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS51953.1"
                     /translation="ELLLLDEPMAGMNLEEKQDMSRFVLDVNDQMGTSIVLIEHDMSV
                     VMDLSDRVVVLDYGRKIADGTPDEVRNDRGVIDAYLGVAH"
     gene            339..1232
                     /locus_tag="VEM33_08735"
     CDS             339..1232
                     /locus_tag="VEM33_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007246480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYS51954.1"
                     /translation="MQFFFEVLIGGLLAGVMYSLVALGFVLIYKASGVFNFAQGAMVF
                     FAALTTVGFISMGAPFWLAVALALLSMVALALFTERLVLRPLVNQPQITLFMATIGLA
                     FFIEGIAQLTWGAHVHALDLGIADEPIEWLMVNTGMNISKFDLVASGIAAALVAVLAA
                     FFNKTRTGRALRAVADDHQAALAVGISLQRIWVIVWMVAGFVALVTGLLWGARNGVQF
                     ALTFLALKALPVLILGGFESIAGAIVGGLIVGASEKLAEVYIGPHVGGGIESWFPYVL
                     ALAFLLVRPEGLFGERRIDRV"
     gene            1239..2345
                     /locus_tag="VEM33_08740"
     CDS             1239..2345
                     /locus_tag="VEM33_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYS51955.1"
                     /translation="MLYREAGQFKTSYAADQQLFPIRQDRIAMAILLAAVFVGVPLAA
                     ATPALAGAIDFNYWFSGVLIPFLIFSLAALGLNILTGYAGQLSLGTAAFMAVGAFAAY
                     NFELRVPGMPMLLSFVLAGIVAALIGIVFGLPSLRIKGFYLAVATLAAQFFVVWALTK
                     FGWLSNYSSSGVITAQKLVVFGYAVDTPVKRYLLVLAIVSVMALAAKNMVRSSVGRAW
                     MAVRDMDVAAEVVGIPIMKTKLLAFAISSFYCGVAGALYAFAYLGTVEPAGFELELSF
                     RILFMIIIGGVGSILGSFLGAAFIVLLPIFMDLSAASIERSFGFALPAGLVSNLTLIL
                     FGALIIFFLVVEPLGLARLWQIAKEKLRLWPFPH"
     gene            2452..>3371
                     /locus_tag="VEM33_08745"
     CDS             2452..>3371
                     /locus_tag="VEM33_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS51956.1"
                     /translation="MLRKIRRAALLGAAAFAVAGTAYSQNEQFVPMNGYWVGPYAAGG
                     SGIFGGFIDYMNMINARDGGINGVKLTYEKCETEYNNARGVECYERTKKRGPTGASLV
                     HPLSTGITYSLIDKGTDDHVPIVSIGYGRTDASDGRVFPWIFPLITNYWSQNTAKIKF
                     IGMKEGGMDKLKGKKIVNIYHDSAYGKETIPVLDAQAKKYGFEVSHVPVAHPGNEQQA
                     QWLQVRQIKPDWVILRGWGVMNPTALKAAAKIGFPRTKIVGVWWSGAEEDVIPAGDAA
                     KGYVAAAFNVSGSNYPVVREIEKFVYAKGKG"
BASE COUNT          554 a         1077 c         1068 g          672 t
ORIGIN      
        1 cggagctgtt gctgctcgat gagcccatgg ccggcatgaa cctcgaggaa aagcaggaca
       61 tgagccggtt cgtgctcgac gtcaacgacc agatgggcac ctccatcgtg ctgatcgagc
      121 acgacatgag cgtggtcatg gacctgtcgg atcgggtggt cgtcctggac tacggccgca
      181 agatcgccga cggcacgccc gacgaggtgc gcaacgaccg cggcgtgatc gacgcgtatt
      241 tgggagtcgc gcattgaaaa ccggggacag accacgtttt cgattttcaa agagagatcg
      301 cggaaaacgt ggtctgtccc cggttttcgc aggctagcat gcaattcttc ttcgaggtgc
      361 tgatcggcgg gctgctggca ggggtgatgt attcgctcgt cgcgctcggc ttcgtgctga
      421 tctacaaggc ctcgggcgtg ttcaatttcg ctcaaggggc gatggtcttc ttcgcggccc
      481 tcacgaccgt gggattcatc tcgatgggcg ccccgttctg gctcgcggtg gcgctcgccc
      541 ttctctcgat ggtggcgctc gcgctcttca ccgagcggct ggtgctgcgc ccgctcgtca
      601 atcagcctca gatcacgctc tttatggcga ctataggcct cgccttcttc atcgagggca
      661 tcgcgcagct cacctggggc gcccatgtcc acgcgctcga cctgggcatc gccgacgagc
      721 cgatcgagtg gctgatggtc aataccggca tgaacatcag caaattcgac ctcgtcgcct
      781 ccgggatcgc ggccgcgctc gtggcggtgc tcgcggcgtt cttcaacaag acccgcaccg
      841 ggcgcgcgct gcgcgcggtc gccgacgacc accaggccgc ccttgcagtc ggcatctcgc
      901 tccagaggat ctgggtgatc gtgtggatgg tcgccggatt cgtcgcgctc gtgaccgggc
      961 tcctgtgggg tgcgcgcaac ggcgtgcagt tcgcgctcac ctttctcgcg ctgaaggctt
     1021 tgccggtgct gatcctgggc ggattcgaat cgatcgccgg ggcgattgtc ggcgggctca
     1081 tcgtgggcgc gtcggaaaaa ctcgccgagg tctacatcgg cccgcacgtc ggcggcggca
     1141 tcgagtcctg gttcccctac gtgctggcgc tcgctttcct cctggtgcgc cccgagggcc
     1201 tgttcggcga acggcgaatc gaccgggtct gacgagtcat gctctatcgc gaagccggac
     1261 agttcaagac cagctacgcc gcggatcagc agctcttccc gatccgccag gaccggatcg
     1321 ccatggcgat cctcctcgca gccgttttcg tcggcgtgcc ccttgccgcc gcgacgcccg
     1381 cgctcgccgg cgcgatcgac ttcaactact ggttcagcgg cgtgttgatc ccgttcctca
     1441 tcttctcgct cgccgccctg ggcctgaaca tcctcaccgg ctacgccggg cagctttcgc
     1501 tgggcaccgc tgcgttcatg gcggtgggcg ccttcgccgc ctacaacttc gagctgcggg
     1561 ttccgggaat gccgatgctg ctgtccttcg tcctcgccgg gatcgtcgcg gcgctgatcg
     1621 ggatcgtgtt cggcctgccg agcctgcgca tcaagggttt ctatctcgcc gtcgcgaccc
     1681 tcgctgcgca attcttcgtc gtatgggcgc tgaccaagtt cggctggctc tccaactaca
     1741 gctcctcggg cgtgatcacc gcccagaagc tcgtcgtctt cggctacgcg gtcgacaccc
     1801 cggttaagcg ctacctgctg gtgctcgcga tcgtgagcgt gatggcgctg gctgcgaaga
     1861 acatggtgcg cagctctgtc ggccgggcct ggatggcggt gcgcgacatg gacgtcgcgg
     1921 ccgaggtcgt cggaatcccg ataatgaaaa ccaagctcct cgcctttgcc atcagctcgt
     1981 tttactgcgg ggtggccggc gcgctctacg cgtttgcgta tctgggcacg gtcgaaccgg
     2041 cggggttcga gctcgagctt tccttcagga tcctcttcat gatcatcatc ggcggcgtcg
     2101 gcagcatcct cggttcgttc ctcggcgctg ccttcatcgt gctcctgccg atcttcatgg
     2161 acctttcggc cgcctcgatc gagcgatctt tcggctttgc cctccccgcg gggctggtgt
     2221 ccaacctgac gctgatcctc ttcggcgcgc tcatcatctt tttcctggtc gtcgagcccc
     2281 tgggccttgc gcgcttgtgg caaatcgcca aggaaaagct gcggctctgg cccttccccc
     2341 actgaaacgg gcgggttttc ccatccgtga caataggggc tatagtaagc gcggctgttc
     2401 attccgataa cctgtagaaa cagggagatt gccatccaaa ggagggttcc aatgttgcga
     2461 aaaatcagac gcgccgcgct cctgggggcc gcggcgttcg ccgtcgcagg cacggcctat
     2521 tcgcagaacg agcagttcgt cccgatgaac ggttactggg tcggccccta cgcggccggc
     2581 ggctccggca tcttcggagg attcatcgac tacatgaaca tgatcaatgc gcgggacggc
     2641 ggcatcaacg gcgtgaagct cacctacgag aagtgcgaga ccgagtacaa caatgcgcgc
     2701 ggcgtcgaat gctacgagcg caccaagaag cggggcccta ccggggcgtc gctcgtgcac
     2761 ccgctctcga ccggcatcac ctattcgctc atcgacaagg gcacggacga ccacgtgccc
     2821 atcgtgtcga tcggctacgg ccgcaccgac gcctccgacg ggcgcgtatt tccgtggatc
     2881 tttccgctca tcaccaatta ctggagccag aacaccgcca agatcaagtt catcggcatg
     2941 aaggaaggcg ggatggacaa gctgaagggc aagaagatcg tgaacatcta tcacgactcg
     3001 gcctacggca aggaaacgat ccccgtgctc gatgcgcagg cgaagaaata cggattcgaa
     3061 gtgagccacg ttccggtcgc gcacccgggc aacgagcagc aggcgcagtg gctgcaggtc
     3121 cgccagatca agcccgactg ggtgatcctg cgcggctggg gcgtgatgaa cccgaccgcg
     3181 ctcaaggcgg ccgccaagat cggctttccg cgcaccaaga tcgtgggcgt atggtggagc
     3241 ggcgccgagg aagacgtgat cccggcgggc gacgcggcca agggttacgt cgctgcggcg
     3301 ttcaacgttt ccggaagcaa ctatccggtg gtgagggaga tcgagaagtt cgtctacgcc
     3361 aagggcaagg g
//
LOCUS       DASPZD010000452         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1781025, whole genome shotgun sequence.
ACCESSION   DASPZD010000452 DASPZD010000000
VERSION     DASPZD010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781025"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VEM33_08750"
     CDS             <1..199
                     /locus_tag="VEM33_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51957.1"
                     /translation="YVQGGDYTVKTVTAEASIFAEKGEKLIWSARTRTTNAQAVKSGA
                     DAATQYIAVILEAMKKDKLLP"
     gene            complement(196..1152)
                     /locus_tag="VEM33_08755"
     CDS             complement(196..1152)
                     /locus_tag="VEM33_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51958.1"
                     /translation="MRNLIRCLALATLLPLAAAGQQFPNKAVRLMVPFPPGGATDIIG
                     RLVSAKMQDVWGQPVVVENKPGAGTVVGTEYVAKSAPDGYTLGMVVTAYVINPSLRAD
                     LPYNTLKDLAGVTQVSVQHLVMAANPSFPANNIAELIALAKKEPGKLAYATPGSGTAM
                     HLSVELLKTSSGIDLVHVPYKGGAPAQQDVMAGRVPILMDVLYAVQPLIKSGRIKVLA
                     LLSPQRAPESPEYPVVSESVPGVSALSLVGIVAPAATPRELVNRISGDIARQVKSSDL
                     TERMKQQGMEPIGSSPEQFDALIRAEMEKWAKVVKQSGAKVD"
     gene            complement(1158..>2219)
                     /locus_tag="VEM33_08760"
     CDS             complement(1158..>2219)
                     /locus_tag="VEM33_08760"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYS51959.1"
                     /translation="SSFGDPSDWLVELTSDDRGELLAALESLERRSRLAPVHALTKSD
                     FPFGPLGEKLDRSYEEVRSGKGFVILHGLPFEGLSLGQFTACAWGAGLHFGRALSQNA
                     QGELVTSVIDATAEDATPRMYRSNLELRPHSDITAMISLACWHKSASGGASIIVSGVT
                     VHDAIRARAPHLLEPLYRGYHYHRLGEEGPDEEPTTPYRTPVFANRNGQVSCRYQRAG
                     IAAGMRERGVPLEERDIEALNLFDQIAADPENRLAFFLERGDMIVINNYAVMHARTRF
                     TNFPEPERQRRLVRMWFDAEGFRDVPREFNLFAENGIPRQEGRRATFDFKKLYRDDPV
                     ATGGVPDLKVSDGEATQGR"
BASE COUNT          351 a          721 c          780 g          367 t
ORIGIN      
        1 gtacgttcag gggggcgatt acacggtgaa gaccgttacc gcggaggcgt cgatcttcgc
       61 ggagaaaggc gagaagctca tctggtccgc gaggacccgg acgaccaacg cccaagcggt
      121 gaaaagcggc gcagacgcgg cgacgcagta catagcggtc atcctcgaag cgatgaagaa
      181 ggacaagctg ctcccctagt ccacctttgc cccggactgc ttcaccacct tcgcccactt
      241 ctccatctcc gcgcggatca gcgcgtcgaa ctgctcaggg ctggagccga tcggctccat
      301 cccctgctgc ttcatgcgct cggtcaggtc ggatgacttg acttggcgcg cgatgtcgcc
      361 gctgatgcgg ttgacgagct cgcgcggcgt ggcggccggc gcgacgatgc cgacgaggga
      421 aagggcgctc acgccgggca ccgattcgga gacgacggga tactcgggcg actcgggcgc
      481 gcgctgcgga gacagcaggg cgagcacttt gatcctgccg ctcttgatca gcggctgcac
      541 cgcgtagagc acgtccatca ggatggggac gcgtccggcc atgacgtcct gctgcgccgg
      601 cgccccgccc ttatagggca cgtgcacgag gtcgatgccc gagctcgtct tcagcagctc
      661 gaccgagagg tgcatcgcgg tgccgcttcc cggggtcgcg taggcgagct tgccgggctc
      721 cttcttcgcg agagcgatca gctcggcaat gttgttcgcg ggaaacgagg gattggcggc
      781 catcaccagg tgctgcaccg agacctgcgt gacgccggcg aggtccttca gggtgttgta
      841 cggcaggtcg gcgcgcaggc tcgggttgat gacgtaggcg gtgaccacca tgccgagggt
      901 gtagccgtcg ggagcggact ttgcgacgta ttcggtgccg accaccgtcc ccgccccggg
      961 tttgttctcg accacgaccg gctggcccca cacgtcctgc atcttcgcgg acacgaggcg
     1021 cccgatgatg tccgtcgctc cgcccggagg aaacggcacc atcagccgca cggccttgtt
     1081 ggggaattgt tggccagcgg ctgcgagcgg caaaagcgtc gcaagtgcaa ggcaccggat
     1141 cagattgcgc atggcgctca acggccttga gtcgcttcgc cgtcgctcac cttgagatcg
     1201 ggaacgccgc ccgtcgcgac cgggtcgtcg cgatagagct tcttgaagtc gaaggtcgcg
     1261 cgccggcctt cctgcctggg gatcccgttc tcggcgaaca ggttgaactc gcgcggcacg
     1321 tcgcggaacc cctcggcgtc gaaccacatg cgcaccaggc gccgctgccg ctcgggctcg
     1381 gggaaattcg tgaagcgcgt gcgtgcgtgc atcacggcgt agttgttgat gacgatcatg
     1441 tcgccgcgct cgaggaagaa ggcgaggcga ttctcgggat cggcggcgat ctggtcgaac
     1501 agattgagcg cttcgatgtc gcgctcctcc agcggaaccc cgcgctcgcg catgccagcg
     1561 gcgatgccgg cgcgctggta gcggcagctg acctggccgt tcctgttggc gaacaccggc
     1621 gtgcggtagg gtgtcgtcgg ctcctcgtcc gggccttcct cgcccaggcg gtggtagtgg
     1681 tagccgcggt agagcggctc gagcaagtgc ggtgcgcgcg cgcggatcgc gtcgtgcacc
     1741 gtgacgccgc tcacgatgat cgaggcgccg cccgaggccg acttgtgcca gcaggcaagc
     1801 gagatcatcg cggtgatgtc cgagtgcggc cgcagctcga ggttgctgcg gtacatgcgc
     1861 ggggtcgcgt cctcggcggt cgcgtcgatc accgaggtga cgagctcgcc ttgcgcgttc
     1921 tgcgagagcg cccgcccgaa gtgcaggccc gcgccccagg cgcaggcggt gaactgcccg
     1981 agcgacaggc cctcgaaggg caggccgtgc agtatgacga agcctttccc cgagcgcacc
     2041 tcctcgtagc tgcgatcgag cttctcgccc agcgggccga aggggaaatc gctcttcgtc
     2101 agggcgtgga ccggggcgag ccggctgcgc cgctccaggg actcgagggc cgcgagcagc
     2161 tcgccgcggt cgtcactcgt cagctcgacc agccagtcgg agggatcccc gaagctcga
//
LOCUS       DASPZD010000453         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1736412, whole genome shotgun sequence.
ACCESSION   DASPZD010000453 DASPZD010000000
VERSION     DASPZD010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736412"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VEM33_08765"
     CDS             complement(<1..1001)
                     /locus_tag="VEM33_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYS51960.1"
                     /translation="MDSLITAAARALAMGDPLGALNRVALRDDAPALALRGIAMAQLG
                     DLTRAKALVRRAARAFGPREAVARARCVVAEAEIALASRDLAWPTKTLAAASATLEAH
                     GDRANAAHARYLEARRLLLIGRLDDAEHKLAQLDLSALTPALRTIHELAVAGISMRRV
                     RAKAAHAALARAEEAARWARIPALAAEVEHALAVLETPAARLIARGEERPLLLEEVEA
                     LLASKALVIDACRNLVRERNMLVPLARRPVLFALARTLGEAWPGDVARNVLLTRAFGA
                     KHADESHRARLRVEIGRLRRLLQAVARVSATKQGFMLSPRGAREVIVLARPIEEQHAA
                     "
     gene            1088..1858
                     /locus_tag="VEM33_08770"
     CDS             1088..1858
                     /locus_tag="VEM33_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HYS51961.1"
                     /translation="MTTHKTGTREQWLAARLKLLDAEKALTRQSDELARRRQELPWVR
                     VEKDYRFDTDEGKASLADLFQGRSQLLVYHFMFGPDYKAGCISCSSIADGFNGIAVHL
                     TNHDVMLWAASRAPLPKLQAFKQRMGWTFPWASSQGGDFNYDFNVSITEEQQRNGGSE
                     YNYRRGGHAMTETTLPPVVIENAARAGAEPIAYVRERPGLSAFVLEDGVVYHTYSTYA
                     RGLDGLWGMYQWLDRAPKGRNEQGVWWRHHDSYEQVAR"
     gene            1855..2136
                     /locus_tag="VEM33_08775"
     CDS             1855..2136
                     /locus_tag="VEM33_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51962.1"
                     /translation="MIATRGETVRASGGGSKVAAHDAAGWLLLAAAPTFAIMALLTGV
                     LGSGRADMLCSPAHDASPLNGMVWMYVLMSAFHSAPWLKLISSRRNGAR"
BASE COUNT          525 a          886 c          882 g          488 t
ORIGIN      
        1 gcggcatgtt gctcctcgat cggccgcgcc agcacgatga cctcgcgcgc gccgcgcggc
       61 gagagcatga aaccctgttt ggtcgcgctc acgcgggcga ccgcctgcaa cagcctgcgg
      121 agccgcccga tctcgacccg caaccgcgcg cgatgggatt cgtcggcgtg cttcgcgccg
      181 aacgcgcggg tgagcagcac gttccgcgcc acgtccccgg gccatgcctc gccgagcgtg
      241 cgcgcaagcg cgaacagcac cggacgcctc gcgagcggca cgagcatgtt tcgctcgcgc
      301 acaagattgc ggcaggcgtc gatgacaagc gccttcgacg cgagcagcgc ctcgacttcc
      361 tctagcagga gagggcgctc ctcaccgcgc gcgatcagcc gggccgcagg tgtctcgagg
      421 acagcgaggg cgtgttcgac ctccgccgcg agcgccggaa tgcgtgccca gcgcgcggct
      481 tcctcggcgc gggcgagcgc tgcgtgtgct gccttggcgc ggacacgccg catagaaatc
      541 ccggcgaccg cgagctcatg tatggtacgc agcgcaggcg taagagctga gagatcgagc
      601 tgcgcaagct tgtgctcggc atcgtcgaga cgcccgatca gaagcaggcg ccgggcctcg
      661 aggtagcgcg catgggcggc gttcgcccgg tcgccatgcg cttcgagggt cgccgatgcc
      721 gcggcgagtg tcttcgtggg ccaggcgagg tcgcgcgaag cgagcgcgat ctcggcctcg
      781 gcaacgacac atctcgcgcg agccacagcc tctctcgggc cgaaggcgcg tgccgcccgc
      841 cgcactagcg ctttcgcgcg ggtgagatcg ccaagctggg ccatggcgat gccgcgaagc
      901 gcgagcgctg gcgcgtcatc gcggagcgcg actctgttca gcgcgccgag gggatcgccc
      961 atcgcgagcg cccgagccgc cgccgtgatc agcgagtcca tcggaatccc gtcacacttg
     1021 taactcccag ggggcccaag cccggtccta gtctaatcca caacggtaca gataacggag
     1081 aatgacgatg acgacacaca agaccggaac acgtgaacag tggcttgcgg cgcggctcaa
     1141 gctgctcgat gcggaaaagg cgctgacgcg gcagagcgat gagctcgcgc gaaggcgcca
     1201 ggagctgccc tgggtccgcg tggaaaagga ctaccggttc gacaccgacg aagggaaggc
     1261 ttcgctcgcg gacctcttcc aggggcgctc gcagctcctc gtctatcact tcatgttcgg
     1321 gccggactac aaggcgggct gcatttcctg ctcttcgatc gccgacggtt ttaacggcat
     1381 cgcggtgcac ctcaccaacc acgacgtgat gctctgggcg gcatcgcgcg cgccgcttcc
     1441 gaagctacag gcgttcaaac agcggatggg ctggaccttt ccctgggcgt cctcgcaggg
     1501 cggcgacttc aactacgact tcaatgtctc catcaccgaa gagcagcagc gcaacggcgg
     1561 cagcgaatac aattaccgtc gcggcggcca cgcgatgacc gagacgacgc ttccgcctgt
     1621 cgtgatcgag aatgcggcca gggccggcgc cgagccgatc gcctatgtgc gcgagcggcc
     1681 gggcctcagc gctttcgtcc tcgaagacgg cgtcgtctat catacctatt cgacctatgc
     1741 ccgcggactg gacgggctct ggggcatgta ccagtggctc gaccgcgccc cgaagggacg
     1801 caatgaacag ggcgtctggt ggcgtcacca cgacagctac gagcaggtgg cgcgatgatc
     1861 gccacgcgag gtgaaaccgt gcgcgccagc ggtggaggca gcaaggtggc tgcgcacgac
     1921 gcagctggct ggcttttgct ggcggctgcg ccgacattcg caatcatggc gttgctcacg
     1981 ggcgttcttg gtagcggccg ggcggacatg ctctgttcgc ccgcgcatga tgcgtcaccg
     2041 ctgaatggaa tggtctggat gtacgtgttg atgagcgcct tccattcggc gccctggctg
     2101 aaactgatct ctagccgacg aaacggcgcc cgctaggcgt gaatgcaacg tatcttaacg
     2161 aacaaacggg aagccatcct aaacggaact gcacctgcct gggagaatga catcagctgc
     2221 tatcctgaaa gaattccgca aatcttcgcc gaggactttc gtcgagtccg aaatattgtc
     2281 aacgctcatg tgaagttcga acgatcggat ttaagcctga cgaactttta cgaccaatat
     2341 cataaatatc tttatatgct ctatcgggat gtccagagtt ggtggggtcg cttaagcgac
     2401 gaatttcctg acctcaatga aatcactcgc ttctcggtag tcgtaaggga cacctcgcct
     2461 tcgcaggcac tgccctgccc ttcaccgagc gtgcctacga cggacagact ggattcctga
     2521 tcgagcgtgc aatcgatgag cgcgctcacg ccaatttaag ccgatcggtt gaagcatcgc
     2581 ggcccttcga cctcagcggc gtggcggctt ctgtgcccat cgcaaaaagg ttttgtatca
     2641 cgagaacatc gaaaggatgg tcggtaattg atttatcgcc gcggccatca agccctcgta
     2701 gctcgtcacg cctccggcag ccgccttcag aataaattca gccacgtacg cctttgttac
     2761 agtagttgct tgttccgcag g
//
LOCUS       DASPZD010000454         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_424325, whole genome shotgun sequence.
ACCESSION   DASPZD010000454 DASPZD010000000
VERSION     DASPZD010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424325"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VEM33_08780"
     CDS             complement(<1..944)
                     /locus_tag="VEM33_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51963.1"
                     /translation="MQTGKATPPAKKLRAYAQAMGSCAARLRPFLDKLRLYVSKLRPY
                     VDKLRPYASKLHRYAVEAVDAVGSWLHRGIVQIGKLSPRTGKRLEAVEAWVCQSDRRK
                     LSVAALVAVLVLGIGFAAIFPEAAGRYGRVIFTSNRVVPLPEETRRDVREKAKQLAAA
                     LDSRLDTKGKLAGETWASAQILVALRESDPDYASRIDVKLIEQYFRSMAGPECACWRR
                     FPQGKHPNHLGVTSWVLWTFAQYGIPAHKSELEFLLSAQGADGGWPLFGGAQEEKFTS
                     SYGTAAAILALHGQAALQPDRGQRKRLAAAVDRGADWL"
     gene            complement(949..1938)
                     /locus_tag="VEM33_08785"
     CDS             complement(949..1938)
                     /locus_tag="VEM33_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HYS51964.1"
                     /translation="MRAAYYERKGPASKVLVLGELPDPQPAPGEVRVKLNFSGINPTD
                     TKLRGGWDGSTEMPFPRVIPHQDGAGVVDAVGPGVPRSRIGERVWIYEAQRGRAFGTA
                     AEYVAIPSENAVALPESASFETGACLGIAGMTAHRCLFQDGGIQGQTVLVAGGGGAVG
                     HAAIQLAKWGGARVATSVSRPEQEKIAREAGADLVVDRKAGDVAARIKAFTRGQGLDR
                     VVEVAFEANLDLNRAVLKANGVISTYSSGPPDSAPRIPFTAIMRQGISMHFILVYAMP
                     REAHQSAARDLNAALETGRYRPHVAGIFKLDETAKAHEAQESGGTVGKLLIGV"
     gene            complement(1935..2426)
                     /locus_tag="VEM33_08790"
     CDS             complement(1935..2426)
                     /locus_tag="VEM33_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS51965.1"
                     /translation="MARSVLKTKFISHGTLGSKDLEATRRFYEEFLGLEVVRTSPVSL
                     MIRLGGNHVYAVVYTKKKERMPRVYHNGLDVDTDAEVDEAWQACHSQAEKWGLHDIDK
                     PSARHGTYSFLFWDADDNAWEILSNPKGGYTWIFEQGDLEGRGHFERGFRHKRPDAKK
                     ETA"
     gene            complement(2431..3426)
                     /locus_tag="VEM33_08795"
     CDS             complement(2431..3426)
                     /locus_tag="VEM33_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS51966.1"
                     /translation="MRLPPRNSMRIATGCLLALVLGAGAMSARAQVAYPSRAVRMVVD
                     TSPGGLTDILGRLAAEGLAQQLGRQVVVENKAGASGNVAIEYVVKSSADGHTLLIGAG
                     GNLVIKPFLEHSLVFDPLVDLVPVFNFAEAPHILVVPNSIAARDLAEFIAYARANPGK
                     VYYGSAGTGSPPHLAADQFARLAGLQLVHVPYKGVGPAFPDLIAGRLQVMSMSYGSAR
                     PYLKSGALRALAAGSKRRLAGLPEVPTSAEAGLPGWEMSAWFGIFAPKDTSAEIVRLL
                     NEKLQAVIEDPKARQRLLEVGAEPLGGTAPAFAERVRADYRMWGQVIKESGIKLE"
BASE COUNT          533 a         1187 c         1179 g          627 t
ORIGIN      
        1 ttgagccagt cagctccacg gtccaccgcg gcggcgagcc gcttcctctg cccgcgatcg
       61 ggttgcagag ccgcttgccc gtgcagggcg agtatggcgg cggccgtccc gtagctcgag
      121 gtgaactttt cctcctgggc acccccgaac agcggccacc ccccgtccgc gccctgggcg
      181 gagaggagaa actcgagctc gctcttgtgg gccgggatcc cgtactgcgc gaaagtccac
      241 agcacccacg atgtgacgcc gaggtgattc ggatgcttgc cttggggaaa ccggcgccag
      301 caggcgcact cgggtccggc catggagcgg aaatactgct cgatcagttt gacgtcgatc
      361 cggctcgcgt agtccggatc gctttccctg agcgcgacga ggatctgggc ggaggcccag
      421 gtctcgccgg cgagctttcc tttcgtgtcc aggcgactgt cgagcgccgc ggcgagctgt
      481 ttcgcttttt cgcggacgtc ccggcgcgtt tcttccggaa gcgggacgac ccggttcgaa
      541 gtgaatatca cgcggccata gcggccggcc gcttccggaa aaatcgccgc gaaaccgatt
      601 ccgagcacga ggactgcgac gagcgccgct acactgagct tgcgcctgtc gctttgacaa
      661 acccaggctt cgacggcttc gagtcgcttc cccgtgcgcg ggctcagctt gcctatctga
      721 acgatccccc gatgcagcca gctcccgacg gcatccaccg cttctacggc gtaccggtgc
      781 agcttgctcg cgtaggggcg cagcttgtcg acgtagggac gcagcttgct gacatagaga
      841 cgcagcttgt cgaggaaggg acgcagccgg gcggcgcagg accccatcgc ctgcgcgtag
      901 gcgcggagct tcttcgcggg tggggttgct ttgccggtct gcatgacctc agacaccgat
      961 gaggagcttg ccgaccgtgc cgccgctttc ctgagcttcg tgtgccttgg ccgtttcatc
     1021 gagcttgaag atgccggcga cgtgcggacg gtagcgcccc gtctcgagcg ccgcgttcag
     1081 gtcgcgcgcc gccgactggt gcgcctcgcg gggcatcgcg tagacgagga tgaagtgcat
     1141 gcttattccc tgccgcatga tcgcggtaaa cgggatgcgc ggcgcgctgt cgggcggtcc
     1201 cgaagagtag gtggagatca ctccgttggc tttgagcacc gcgcggttga ggtcgaggtt
     1261 cgcttcgaag gcgacttcca ccacgcgatc cagtccctgc cctcgcgtga acgccttgat
     1321 tctggcggcg acgtcccctg ctttccggtc gacgacgaga tcggcacccg cctcgcgcgc
     1381 gattttctcc tgctcgggcc ggctcacgct ggtggcgacg cgcgctccgc cccatttcgc
     1441 gagctggatc gcggcgtgac cgacagcgcc gccgccgccc gcgacgagga cggtctgtcc
     1501 ctggatgccg ccgtcctgga aaaggcagcg gtgcgcggtc atcccggcga tgccgaggca
     1561 ggctccggtc tcgaagctcg cgctctcggg aagcgcaacc gcattctccg aagggatcgc
     1621 gacgtattcg gccgcggttc cgaaagcgcg gccgcgctgc gcttcgtaga tccagacacg
     1681 ctcgccgatc ctcgatcgcg gcacgcccgg cccgaccgcg tcgacgacgc cggcgccgtc
     1741 ctgatgcgga atcacgcgcg gaaacggcat ttccgtgctg ccgtcccagc cgccgcgcaa
     1801 tttggtgtcg gtcggattga ttcccgagaa attcagcttg acgcgcactt cgcccggcgc
     1861 gggctgcggg tcgggcagct cgccgagaac gaggactttc gaggcgggcc ccttgcgctc
     1921 gtagtacgcc gccctcaagc ggtttccttc ttcgcgtccg ggcgcttgtg gcggaagccg
     1981 cgctcgaaat gcccgcggcc ctcgagatcg ccctgctcga agatccaggt gtagccgccc
     2041 ttgggattgg agaggatctc ccaggcgttg tcgtcggcgt cccagaaaag gaagctgtag
     2101 gttccgtggc gggccgaggg cttgtcgatg tcgtgcagcc cccacttctc cgcctgcgag
     2161 tggcacgcct gccaggcttc gtccacctcg gcgtcggtgt cgacgtccag gccgttgtgg
     2221 tagacgcgcg gcatgcgctc cttcttcttc gtgtacacga cggcgtacac gtggttgccg
     2281 ccgaggcgga tcatgaggga cacgggactg gtgcgcacca cttccagacc gaggaactcc
     2341 tcgtagaaac ggcgcgtcgc ctcgagatct ttcgagccga gcgtgccatg cgagatgaat
     2401 ttcgtcttga gcaccgagcg agccatggcg ctactccagt ttgattcccg attccttgat
     2461 gacctgcccc cacatcctgt agtcggcgcg cacgcgctcg gcaaatgccg gagccgtacc
     2521 gccgagcggc tcggcgccga cctcgagcag ccgctgcctc gctttcgggt cctcgatcac
     2581 cgcctggagc ttctcgttca gcagacgcac gatttcggcg ctcgtatctt tcggcgcgaa
     2641 aatgccgaac caggcgctca tctcccagcc cggcagtccc gcctccgcgg aggtcggcac
     2701 ctcgggcagc ccggccaggc gccgcttcga tccggccgcg agggcgcgca gcgcgccgct
     2761 cttgagatac ggccgcgccg agccgtagct catcgacatg acctgcaggc ggcccgcgat
     2821 cagatccggg aacgcgggcc ccacgccctt gtacggcacg tgcacgagct gcaggccagc
     2881 gaggcgcgcg aattgatcgg cggcgaggtg cggcgggctg ccggtccccg ccgagccgta
     2941 gtagaccttg cccgggttcg ctctggcgta cgcgatgaac tccgcgagat ccctggccgc
     3001 aatcgaattc gggacgacga ggatgtgcgg cgcctcggcg aagttgaaca ccgggacgag
     3061 atcgacgagc ggatcgaaaa cgagcgagtg ctcgaggaaa ggcttgatga cgaggttccc
     3121 gccggcgccg atcagtagcg tgtggccgtc ggcggacgat ttcaccacgt attcgatcgc
     3181 gacgttgccc gatgcgcccg ccttgttctc gaccacgacc tgccggccga gctgctgcgc
     3241 gaggccctcg gcggcgaggc gcccgaggat gtcggtgagc ccaccggggg acgtgtcgac
     3301 caccatgcgg accgcgcggc tcggataggc gacctgggcc ctggcggaca tcgctcccgc
     3361 gcccaggacg agggcgagga ggcacccggt tgcgatccgc atggaattgc gcggcggtaa
     3421 gcgcatcgtt tcttagtcga ttcggatgtt cgcttttttc acgatcgccg cgtagcgctc
     3481 gtgctcgcgc ttcaagagcg cgccgagatc ctcgggcgag ctcgac
//
LOCUS       DASPZD010000455         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_291038, whole genome shotgun sequence.
ACCESSION   DASPZD010000455 DASPZD010000000
VERSION     DASPZD010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291038"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /gene="zapD"
                     /locus_tag="VEM33_08800"
     CDS             complement(<1..416)
                     /gene="zapD"
                     /locus_tag="VEM33_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HYS51967.1"
                     /translation="MITYEYPLNERIRTLLRLEDLFERADHFVSGEDSRAHHAALITL
                     FEILEVSGRAELKSDLLQELERQKQVLLGFRNNPQIAETVLREVISDIERSSAALFAM
                     PGKIGQHLRENDWLMSIKQRTGIPGGVCEFDLPSYH"
     gene            complement(510..1127)
                     /gene="coaE"
                     /locus_tag="VEM33_08805"
     CDS             complement(510..1127)
                     /gene="coaE"
                     /locus_tag="VEM33_08805"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705097.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYS51968.1"
                     /translation="MAFTVGLTGGIGSGKSMVADLFAKRGVAVIDTDEIAHELTRPGG
                     EAIEAVRMAFGASSIGADGALDRDRMRKLVFADAAARKKLEGILHPLIRKESARRSAR
                     ARSPYAILVVPLLVEGGVDRSRYSRVLVVDCSEEQQVERAMRRSGLSEKEVRAILAAQ
                     ATREQRLARADDVIDNSGTPGALERQVSHLDEKYLTLAAQSKTAS"
     gene            complement(1133..1993)
                     /locus_tag="VEM33_08810"
     CDS             complement(1133..1993)
                     /locus_tag="VEM33_08810"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HYS51969.1"
                     /translation="MSEIFKTAAHPAVFPWLSLVVGLVVGSFLNVVIHRLPKMMERDW
                     QEGCAELSGETLAPKEEFNLFVPRSRCPACGHGITVAENIPLVSWAMLRGRCSACKAP
                     ISLRYPLVELLAGVGGFASATHFGFGLAAAGAAIFIWCTIALAFIDQETGYLYDDITL
                     PLLWLGLLLNLGGTYVPIGDAVVGAAAGYLSLWTVYQAFKLLTGRKGMGFGDFKLNAA
                     VGAFLGWKMLPLVILVSSVVGSIFGIGQMFAAKRGWEWNFKFHFGPYLAIAGIIAMFW
                     GRQIVDWYLR"
BASE COUNT          412 a          711 c          679 g          368 t
ORIGIN      
        1 aagtggtatg agggcagatc gaattcgcaa accccgccgg ggattccggt gcgctgcttg
       61 atcgacatca gccaatcgtt ctcgcgaagg tgctgcccga tcttgccggg catggcgaag
      121 agcgccgcgc tcgagcgctc gatgtcggaa atgacctccc ggagcacggt ctccgcgatt
      181 tgagggttgt tgcgaaaccc gagcaggacc tgcttctgcc gttccagttc ctgcagcagg
      241 tccgacttga gctccgcgcg gccggagact tccaggattt caaacagggt tatcagcgcg
      301 gcgtggtggg cgcgcgagtc ttccccggaa acgaagtgat cggcgcgctc gaacaggtct
      361 tccaggcgca gcagtgtccg gattcgttcg ttgagcggat actcgtaggt gatcaccgtg
      421 acacgcgcga ctggaagggt aggcggtcga ttccggggga gcctcgcggg attctccccg
      481 atccgccccg aattgacaac gggcgagcgc tatgacgctg tcttggattg cgcggcaagc
      541 gtgagatatt tctcatccag atgcgacact tggcgttcga gcgccccggg tgttccgctg
      601 ttgtcgatca cgtcgtcggc tcgcgccagg cgctgctcgc gcgtggcctg cgcggcgagg
      661 atggcccgca cttccttctc cgagaggccg cttcgccgca tggcgcgctc gacctgctgc
      721 tcctcgctgc aatcgaccac gagcacccgg gagtagcgcg atcggtcgac accgccctcc
      781 accaggagcg ggaccacgag aattgcgtag ggactccgcg cgcgcgcgct ccggcgcgcg
      841 ctttccttgc gtatcagggg atgcaggatc ccttcgagct ttttcctcgc cgccgcgtcc
      901 gcgaacacga gcttgcgcat ccggtcgcga tcgagagcgc cgtccgcgcc gatcgagctt
      961 gcgccgaacg ccatgcgtac cgcctcgatc gcctcgcccc ccgggcgcgt gagctcgtgg
     1021 gcgatctcgt cggtatcgat cacggcgacc ccgcgcttgg cgaacagatc cgcgaccatg
     1081 cttttgccgc tgccgatgcc gcccgtgagt ccgacggtga atgccatcgc cgtcagcgca
     1141 gataccagtc gacgatctgc cggccccaga acatggcgat gatcccggcg atcgccagat
     1201 aaggcccgaa atgaaacttg aagttccact cccagccgcg cttcgcggcg aacatctggc
     1261 cgatgccgaa gatcgatccg acgaccgacg agaccaggat cacgagcggc agcatcttcc
     1321 agccgaggaa ggcgccgacc gcggcgttca acttgaaatc gccgaagccc atgcctttcc
     1381 ttccggtcag gagcttgaag gcctgataga cggtccacag cgagaggtag cccgccgccg
     1441 cgccgactac cgcatcgcca atcggcacgt acgttccccc caggttcagc agcaagccaa
     1501 gccacaggag cggcagcgtg atgtcgtcgt agagataccc ggtctcctgg tcgatgaacg
     1561 cgagggcaat cgtgcaccaa atgaagatcg cggcgccggc cgcggcgagg ccgaatccga
     1621 agtgcgtggc ggaggcgaaa cctccgacac ccgccagcag ctcgaccagc ggatagcgca
     1681 ggctgatggg agctttgcac gcggaacagc ggccgcgcag catcgcccac gaaacgagcg
     1741 gaatgttctc ggccacagtg atcccgtgac cgcaggcagg acagcgcgag cgcggcacga
     1801 acaagttgaa ctcttccttc ggcgcgagcg tctcgccgct cagctcggcg catccctcct
     1861 gccagtcgcg ctccatcatc ttcggcaagc gatgaatgac cacgttcagg aacgagccga
     1921 ccacgagtcc gaccacgagg gagagccagg gaaacaccgc cgggtgtgcg gccgttttga
     1981 aaatctcgct catcggatca aatgggttac ggccggaccg taaccatttc gacgaggcgc
     2041 gtgatgaaaa taatgctaga ccgcttgacc gagcttgaag atgggcaggt acatcgcgat
     2101 caccatgccg ccgatcacca cgccgagcac taccatgatc atcggttcca tcagactggt
     2161 cagcgcgtcg
//
LOCUS       DASPZD010000456         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1261496, whole genome shotgun sequence.
ACCESSION   DASPZD010000456 DASPZD010000000
VERSION     DASPZD010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261496"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VEM33_08815"
     CDS             <1..508
                     /locus_tag="VEM33_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYS51970.1"
                     /translation="SALEVGMVGREGMVGVPLSLGADVSPVRALVQGAGVAMRMKAAR
                     FSHELRKSPQLQQEVNRYTGVLMAQITQTAACNRFHVVEARLARWLLMTRDRLRSDEF
                     PLTHEFLGHMLGVRRVGVTKAARALQGRKLIRYSRGKIQILNRRGLKASACSCYELVK
                     DMYDDVRD"
     gene            complement(815..>2081)
                     /locus_tag="VEM33_08820"
     CDS             complement(815..>2081)
                     /locus_tag="VEM33_08820"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYS51971.1"
                     /translation="ARLLAELFARATAPEQDFLARLLLGELRQGALEGIMLDAIAKAA
                     NLRPERVRTAVMRAGGLPAVAKAALTEGEAGLARFALRVFQPVQPMLAQPADGVADAI
                     ERLGRAAFEWKLDGARVQAHKSGGEIRVYTRSLNEVTAAVPEIVAALQGCSAREVMLD
                     GETIALKPDGTPYPFQETMRRFGRKLDVEALRAAFPLSVFFFDCLLAEGEDLTARPAR
                     ERFDALAKFLPVKILIPRLVTGDKEAAQTFYDEALARGHEGVMAKALEAPYEAGSRGA
                     GWLKIKAAHTLELAVIAVEWGSGRRKGWLSNLHLGARDPATGAFVMLGKTFKGMTDKM
                     LAWQTERLLSLETSREGHVVHVRPELVAEVAFNEIQASPHYPGGFALRFARVKRYRED
                     KSARDADTIETVRTLYQRQLHRRPGESSG"
BASE COUNT          358 a          676 c          682 g          365 t
ORIGIN      
        1 atcggccctg gaagtcggca tggtcggccg tgagggcatg gtcggcgttc ctctgtccct
       61 gggagcggac gtttcgcctg tgcgcgcgct ggtgcaggga gcgggagtgg ccatgcggat
      121 gaaggcggcg cgcttttccc atgaactccg gaaaagcccg cagctgcagc aagaggtcaa
      181 tcgctatacg ggcgtattga tggcccagat cacccagacc gccgcgtgca accgcttcca
      241 tgtcgtagag gcgcgccttg cgcgttggct cctgatgacg cgggaccggc tacggtcgga
      301 cgaattccct cttacgcacg aattcctcgg gcatatgctg ggagtgcggc gggtcggcgt
      361 caccaaggcc gcgcgcgcgc tgcagggaag aaagctgatc cgttacagcc gcggcaagat
      421 ccagatcctc aatcgacgag gcctcaaggc ctcagcctgc tcctgctacg agctcgtcaa
      481 ggacatgtac gacgacgtac gcgattaggg cagcgcaaaa aaatgcccgg tacgggattg
      541 ccgttaccgg gccagatact acgagtgtct atcagggagg agatacacac ccgactcgat
      601 cgtccccttt tgctcctagg cactctgtgc gttagcgcac agaaggggcc attatttgat
      661 cacttttaat cgcgcgcggg ctattgtgaa acaacttgcg cgaaagcgct ccagacggac
      721 tactgtcctg tcgctcgatc acgacgttcg tgtcgggaaa accctgacac aaatgtcgca
      781 atgacagaac aagctatcaa agagccgggg actctcagcc ggagctttcg cccggtcgcc
      841 gatgcaactg ccgctgatac aaggttcgca cggtttcgat cgtgtcggcg tccctagcgc
      901 tcttgtcctc gcggtagcgc ttgacgcgcg caaagcgcag cgcgaacccg cccggataat
      961 gcgggctcgc ctggatttcg ttgaacgcga cctcggcgac gagctcgggc cgcacgtgca
     1021 cgacgtggcc ctcgcgcgat gtttcgagtg agagcagccg ctcggtctgc cacgcgagca
     1081 tcttgtcggt catgcctttg aaggtcttgc cgagcatgac gaaggcgccg gtggcgggat
     1141 cgcgcgcgcc caagtgcagg ttgctgagcc agcccttgcg gcggccgctg ccccattcaa
     1201 ccgcgattac ggcgagctcg agcgtgtgcg ccgctttgat cttgagccag ccggcgccgc
     1261 ggcttccagc ctcgtacggg gcctccaggg ctttcgccat gacgccttcg tggccgcgcg
     1321 cgagcgcttc gtcgtaaaag gtttgcgcgg cttccttatc gccggtcacc aggcgtggga
     1381 tgagaatctt caccggaaga aatttcgcga gcgcgtcgaa gcgttcccgc gcgggtctcg
     1441 cggtgaggtc ctcgccctcg gcgagcaggc agtcgaagaa aaagacgctc aaggggaacg
     1501 ccgctcgcag cgcctccacg tcgagcttgc gcccgaagcg gcgcatggtc tcctggaaag
     1561 ggtagggcgt gccgtcgggc ttgagcgcga tcgtttcgcc gtcgagcatc acttcgcgcg
     1621 cggagcatcc ctgcagcgcc gcgacgatct cgggcacggc ggcggtgacc tcgttcaggc
     1681 tgcgcgtgta cacgcggatt tcgccgcccg acttgtgcgc ctgcacgcgc gcgccgtcga
     1741 gcttccactc gaaagccgcg cgtcccaagc gctcgatggc gtcggcgacg ccatcggcgg
     1801 gctgggcgag catcggctgc accggctgaa aaaccctgag cgcaaagcgc gcaaggcccg
     1861 cttcaccttc ggtgagggcg gccttcgcga ccgcaggcag gccgcccgcg cgcatcaccg
     1921 ccgtccgcac ccgctcgggc cgcaggttcg ccgcctttgc gatggcgtcg agcatgatcc
     1981 cttcgagcgc gccctggcgc agctctccga gcaacagccg cgcgagaaag tcctgctcgg
     2041 gcgcggtcgc gcgcgcgaaa agctccgcga gcaggcgcgc g
//
LOCUS       DASPZD010000457         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_951188, whole genome shotgun sequence.
ACCESSION   DASPZD010000457 DASPZD010000000
VERSION     DASPZD010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951188"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..935
                     /gene="rpoE"
                     /locus_tag="VEM33_08825"
     CDS             336..935
                     /gene="rpoE"
                     /locus_tag="VEM33_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HYS51972.1"
                     /translation="MDERDLDQQLVEQAQRGDKHAFELLVSKYQRKLARLLSRFIRDP
                     TEVEDVAQETFIKAYRALPSFRGDSAFYTWLYRIGINTAKNYLVALGRRVPTTTEFDS
                     EDAEGFEGGEQLRDINTPESMLMSREIANTVNDTLMHLPEELRNAITLREIEGLSYED
                     IATIMNCPIGTVRSRIFRAREAIAEKLRPLLDTRKDQRW"
     gene            938..1453
                     /locus_tag="VEM33_08830"
     CDS             938..1453
                     /locus_tag="VEM33_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HYS51973.1"
                     /translation="MKEKLSALIDGELQSDNLHAHLERLRADPELRGAWDTYHLIGDA
                     LRGQVGPRIADRVLDRLREEPTVLAPRPERSPSRRLGWYAMSAAASIAAVAFVVWTAS
                     PGWRAEPQLAGGPEAPATAAPVTLVSSSVAPAEIENYLLAHRPYSHTSAMQGIAPYGH
                     SVAHERRAARE"
     gene            1450..2436
                     /locus_tag="VEM33_08835"
     CDS             1450..2436
                     /locus_tag="VEM33_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HYS51974.1"
                     /translation="MTLPFASCVWRRIRIPLFLLPVSAAIAQTEPTALQWLQRIYAAT
                     EKLSYSGTFVYQQGQQVETSRITHVVEASGPRERLETLDGVPREIIRTGDEVVCYLPA
                     SLTVKIDKQPGRRPFPAILSEQLKDVSENYTIRKGEIERVGGYSCQVLALEPKDRMRY
                     GHRLWADLNTGMLLKAKTFDEKNELLEQFAFTQIKIGGHIGRDQLKARYSRESRDWRV
                     EDSGAKRANLAEAGWTIRSSPAGFRTVAELMRTMGGIGGVGHIVLSDGLAAISVFIEP
                     ATSRQPAPQPGLVRQGAVNVYMRPVGGYWVTVVGEAPAESVKYIANAVEYRK"
     gene            2527..>2724
                     /locus_tag="VEM33_08840"
     CDS             2527..>2724
                     /locus_tag="VEM33_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine peptidase"
                     /protein_id="HYS51975.1"
                     /translation="MARVLFICLLLGLPSALIAQGRELPDFTDLAERQGPTVVNISTT
                     QMRERRASPQIPNLEEDDPLYD"
BASE COUNT          527 a          867 c          875 g          455 t
ORIGIN      
        1 cgcagcgacc attttgtttc cgcagtactc gttgtcatgg ggctcgatgt ggccgggcac
       61 ggccgcaaat ggctggtgct cttgggcgat tcggccctgc ccgaagactt tcggcgcctt
      121 cgggtctggc ttcgttggcg ccgcgatgtc ggatccggaa gggaccggac tcgggccagc
      181 ccggaataag cttgcgggag gctgaactat tcttcggggg gcgagtctct agctgcaggg
      241 tgccagggtc cgcgcacgta ctgcggtata ctggccgccg cctgtggaca acttagaaca
      301 gaagcgcaac accgcttcgt cagggaaggc ggcggatgga tgagcgggat ctcgaccagc
      361 agctggtcga gcaggcgcag cgaggcgaca agcatgcctt cgagttgctg gtgtcgaaat
      421 accagcgaaa gctggcgcgg ttgctgtccc ggttcatccg ggacccgaca gaggtcgagg
      481 atgtcgcgca ggaaacattc atcaaagcct accgtgcgct gccttcgttc cggggagaca
      541 gcgcgtttta tacgtggctg taccggatcg gcatcaacac cgcgaagaac tacctggtgg
      601 cgctggggcg gcgcgtcccg accacgaccg agttcgattc cgaggacgcg gaagggttcg
      661 agggcggcga gcagttgcgc gacatcaaca cgcccgagag catgctgatg tccagggaga
      721 tcgcgaacac cgtcaacgac accttgatgc acctgcccga agagctgcgc aacgccatca
      781 cgctgcgcga gatcgaaggc ctgagttacg aggatatcgc aacgatcatg aattgcccga
      841 tcggcacggt tcgttcgagg atattccgtg cgcgcgaggc aatcgccgaa aagctgcgac
      901 ccctgctcga cacacgcaag gaccaaaggt ggtgaccatg aaggaaaagc tatcggcgct
      961 gatcgacggc gagctccaga gcgacaatct tcacgcgcac ctcgaacggc tccgagcaga
     1021 ccctgaattg cgcggcgcat gggataccta ccacctgatc ggcgacgcgc tcaggggcca
     1081 ggtcggcccg aggatcgccg atcgcgtgct cgaccgcctt cgcgaggagc cgacagtcct
     1141 tgccccgcgg ccggaaagga gcccttcgag gcgcctcggc tggtacgcca tgagcgcagc
     1201 cgccagcatc gccgcggtgg ctttcgtcgt gtggactgct tcccccggct ggcgcgccga
     1261 gccgcagctc gcaggcggtc cggaggcgcc cgccacggcg gccccggtca ccctggtctc
     1321 ctccagtgtc gcgcccgccg aaatcgagaa ttacctgctc gcccatcggc cttattccca
     1381 cacgagcgcc atgcaaggaa tcgcacccta cggccacagc gtggctcacg agcgcagggc
     1441 ggcaagggaa tgacgcttcc cttcgcgtcg tgcgtgtggc ggaggataag aatcccgctg
     1501 ttcctgctcc cggtatcggc ggcaatagcc cagacggaac ccaccgcgct gcagtggctt
     1561 caacgcatct acgccgcaac ggaaaagctc agctactccg gcacgttcgt ctaccagcag
     1621 ggccagcagg tcgaaacctc gcgcatcacc cacgtcgtcg aggcttcggg accgcgcgag
     1681 cgcttggaaa cgctggacgg ggtaccacgc gagatcatcc gtaccggcga cgaagtcgtt
     1741 tgctacctgc ccgcgagcct gacggtgaag atcgacaagc aacccggccg ccggccgttt
     1801 cccgcgatcc tttcggagca gctcaaggac gtgtcggaga actacaccat caggaaaggc
     1861 gagatcgagc gcgtcggagg ctacagttgc caggtcctcg cgctggagcc gaaagaccgg
     1921 atgcgatacg gtcaccggct ttgggccgat ctcaataccg gcatgctgct caaggcgaag
     1981 accttcgacg agaagaacga gctcctagag cagttcgcat tcacgcagat caagatcggc
     2041 ggccacatcg gccgcgatca gctgaaggca cgctattccc gcgaaagccg ggactggcgc
     2101 gtcgaggatt ccggcgcgaa acgcgccaac ctggccgaag ccggatggac gatccgttcc
     2161 agtcccgcgg gttttcgcac ggtcgccgag ttgatgcgca ccatgggagg catcggagga
     2221 gtcggccata tcgtcctgtc cgacgggctt gcagccatct cggtattcat cgagcccgcg
     2281 acgagcaggc agccggcgcc gcagccgggt ctggttcggc agggggcggt caacgtctac
     2341 atgcgccccg tcggcggcta ctgggtcacg gtggtcggcg aagcgcctgc agagagcgta
     2401 aaatacatcg ccaacgcggt cgaataccgc aagtaaaacc cggcgacctc cattagaatc
     2461 ggtccggctc ccggtagccg gctggtgtcg cggttttctg ccggctcgat tccgaggatc
     2521 attttgatgg cgagagtact gttcatctgt ctgttgctag gtttgccctc cgcgctcatc
     2581 gcgcagggac gcgagctgcc cgatttcacc gacctcgccg agaggcaggg ccccaccgtc
     2641 gtcaatatca gcaccaccca gatgcgcgag cgccgcgcgt cgccgcagat tccgaatctc
     2701 gaggaagacg atccgctcta cgat
//
LOCUS       DASPZD010000458         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_654488, whole genome shotgun sequence.
ACCESSION   DASPZD010000458 DASPZD010000000
VERSION     DASPZD010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654488"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="VEM33_08845"
     CDS             <1..1831
                     /locus_tag="VEM33_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HYS51976.1"
                     /translation="LRIRARVRQPVARGFSTKPAPRPSAPASVPEASSAHGTPLLLLY
                     GSNSGASEAFARRVASDGQARGYAAKVAPLDDYTGRLPKEGAAIIVTASYNGQSPDNA
                     RKFATWLAGVPDASLVGVRYALFGCGNRDWTTTYQSVPKEIAGHLDRAGAQALVARGE
                     GDARGDFFGDFEQWYGPLWERLGAGLGVAARPTVGGGPLYDVEAVPTAEVELVRENKL
                     DFSTVIENRELVDLTSPFGRSKRHIEFALPPGVTYAAGDYLAVLPENHPGLVERAARR
                     LGTRPGAAVILRSNRGAMAASLPTDHPVSIQELLGRHVELSAPATRKDVERLAERAQD
                     KAQQAALNELASDPARYQAEVLKKRASVLDLLDAYPSCELSLGEFLELLPAMRVRQYS
                     ISSSPRANPSRCTVTVAIVKAPAWSGQGEFHGTCSSFLARLRAEDQVPIAIRTPNVPF
                     HPPALNSTPVVMICAGTGLAPFRGFLQERALRHAHGEPAGPALLFFGCDHPDVDLLYR
                     EELEQWEREGVVTLYPVFFRKPDGEVTFVQHRLWKDREKVRELYRQGAIFFLCGEGQY
                     MAPAVRETLAKIYQETAGCTGAEASSWLLEMEHKGRYVPDVFA"
     gene            complement(1989..>2513)
                     /locus_tag="VEM33_08850"
     CDS             complement(1989..>2513)
                     /locus_tag="VEM33_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS51977.1"
                     /translation="YFPGLERRMVDRIRDLRGRYGRKVSLIGWSLGGIFARQVAKLVP
                     DDIRTVITLGSPFSGNPRASHAWRLYELTSGYRVDDQSNAFARTLAEPPPVPTTAIYS
                     RSDGICAWECCVEKRTDKSESIEVHSSHCGLGHNPAAVYAIADRLAQREGEWKPFGRG
                     GWRAFVYPEPAQVS"
BASE COUNT          404 a          839 c          853 g          417 t
ORIGIN      
        1 gctcagaatc cgcgcccgcg tcaggcagcc ggtagctcgc gggttttcga cgaagcccgc
       61 gccgcggccg agcgcgccgg cgagcgtccc ggaagcatcg agcgcccacg gcacgccgct
      121 gctgttgctt tacggctcga attcgggcgc ttcggaagcc ttcgcccgtc gggtcgcgag
      181 cgacggccaa gcccgcggct atgcggccaa ggtcgccccg ctcgacgatt acaccggcag
      241 gctgcccaag gaaggtgcgg ccatcatcgt cacggcgtcc tacaacggcc agtcaccgga
      301 caacgcacgc aagttcgcga cctggctcgc cggcgtgccc gacgcgtcgc tcgtcggcgt
      361 gcgttatgcg ctgttcggct gcggcaatcg cgattggacg acgacctacc agtcggtacc
      421 caaggagatc gccggccatc tcgatcgcgc cggcgctcaa gcgctggtgg cgcgcggaga
      481 gggagacgcg cgaggggatt tcttcggcga cttcgaacag tggtacggcc cgctgtggga
      541 acggctcggc gccggtctcg gcgtggcggc gcggccgacg gtcggcggcg gcccgcttta
      601 cgacgtcgag gcggtgccga ccgccgaagt cgagctggtg cgggagaaca agctcgactt
      661 ctccaccgtg atcgagaatc gcgagctggt ggacctgacg tcgccgttcg ggcgctcgaa
      721 gcgccacatc gagttcgcgt tgccgccagg cgtgacctac gccgccggcg attatctcgc
      781 cgtgctgccc gaaaatcacc cgggcctggt cgagcgcgcc gcgcgccggc tcgggacacg
      841 accgggcgcg gccgtgatcc tgcgctcgaa ccgaggcgcc atggccgctt ccctgccgac
      901 cgaccatccc gtctccatcc aggagttgct gggacgccat gtcgagctct cggccccggc
      961 aacacgaaag gacgtcgagc gattggcgga aagggcgcag gacaaagcgc agcaggcggc
     1021 cctgaacgag ctcgcgtcgg atcccgcgcg ctatcaggcg gaagtcctca agaagcgcgc
     1081 gagcgtgctc gacctgctcg acgcctatcc gtcatgcgag ctgagcttgg gagaatttct
     1141 cgagctgctt cccgccatgc gggtgcgtca atactcgatt tcctcctctc cgcgcgcgaa
     1201 cccttcccgc tgcacggtga cggtggcgat cgtcaaggcg ccggcctggt cgggccaagg
     1261 ggagtttcac gggacgtgct ccagcttcct cgcgcgcctg cgcgccgagg atcaggtgcc
     1321 gatcgccatc cgcacgccca atgttccgtt tcatcctccg gcgctcaaca gcacgcccgt
     1381 cgtcatgatt tgcgccggga ccggactggc gccgtttcgc gggttcctgc aggagcgagc
     1441 cttgcgccac gcccacggcg agcccgcggg tccggcgctg ctgttcttcg gctgcgacca
     1501 ccccgacgtc gacttgctct atcgcgagga gctcgagcaa tgggagcggg agggcgtcgt
     1561 cacgctgtat ccggtgttct tccgcaagcc cgatggcgag gtgacgttcg tgcagcatcg
     1621 cctatggaag gaccgagaaa aggtccgcga gctgtaccgg cagggcgcga tcttcttcct
     1681 gtgcggggag ggtcaataca tggcgccggc ggtccgcgag acgctggcaa aaatctatca
     1741 ggagacggcc ggctgcacgg gcgccgaggc gtcgagctgg ctgctcgaga tggaacacaa
     1801 gggccgctac gtgccggacg tcttcgcttg aagatggggg ctggccttct cgtttggctc
     1861 ccgcgctgcg gatatcgcta gagcattttc cggcaacgcg ggaaccggta gaaaacgcga
     1921 caaatatcga atcccagcgc gtcgcgattc aagaggatcg gaacgcgctc cagtcccgca
     1981 gatggcagtc aggagacctg cgccggctcg ggatacacga aagcgcgcca gccgccgcgg
     2041 ccgaacggct tccattcgcc ctcgcgctgg gcgaggcggt cggcgatcgc atagaccgcc
     2101 gccggattgt ggccgagccc gcaatggctg ctgtggactt cgatgctttc gcttttgtcc
     2161 gtccgtttct cgacgcaaca ctcccaagcg caaatgccgt cgcttcggct gtagatcgcc
     2221 gtcgtgggca cggggggcgg ctccgcaagc gtgcgagcaa acgcattgct ctggtcgtcg
     2281 acgcgatatc cgctggtgag ctcgtatagc cgccaggcgt gcgaggctct gggattgccc
     2341 gagaacggac tgccgagcgt gatcaccgtt cggatgtcgt cgggcaccag ctttgcgacc
     2401 tgccgcgcga agatgccacc gaggctccag ccgatcaggc tgacttttcg cccgtagcgg
     2461 ccgcgcaggt cgcggatgcg atcgaccatc cgtcgctcca agccggggaa gta
//
LOCUS       DASPZD010000459         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1436456, whole genome shotgun sequence.
ACCESSION   DASPZD010000459 DASPZD010000000
VERSION     DASPZD010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436456"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VEM33_08855"
     CDS             complement(<1..430)
                     /locus_tag="VEM33_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003276172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYS51978.1"
                     /translation="MARVTRRVPDTLAGLRLDKALARMFPEHSRNRLQRWLKDGHVSL
                     DSHAAKPKQKIWGGERVEIRSQPGPAETAFRAEPIALDVVYEDDDVLVINKPAGLVVH
                     PGSGNWKGTLLNALLHRLPQLAAIPRAGIVHRLDKDTSGLL"
     gene            465..1229
                     /locus_tag="VEM33_08860"
     CDS             465..1229
                     /locus_tag="VEM33_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HYS51979.1"
                     /translation="MKRSVALFALLLAACAFVDKHDPQSGWTAEKLYREAKDALDSGQ
                     YDLAIKRYEILEARFPYGRYSQQGQLEIAYAYYKQNEPASAVAATERFVKLHPNSPNV
                     DYAHYLKGLAYFNEDLGILGRMARQDRTERDPKSARESFDAFKELVQRYPDSRYAPDA
                     MARMKYLVNALASHEVHVARYYMKRGAYVAAANRAQYALKNYPQAPAQEEALLIMIQA
                     YDVLGMPELRDDAERILRKNFPDSAQLKSLLTQLGR"
     gene            complement(1210..>2774)
                     /locus_tag="VEM33_08865"
     CDS             complement(1210..>2774)
                     /locus_tag="VEM33_08865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HYS51980.1"
                     /translation="RSGDKSELADVPEEAPAWNLATSTRENCLGQSCPRFRDCFVMAA
                     RREALAADLVVVNHHLFFADVMLKDEGMAELLPACNTVIFDEAHQLPETATLFFGESL
                     STGQIIELTRDVRAEVLTAAKDMPDPLRLTAALDKAARDLRLALPNEGARFTAASVGR
                     KREVSDALAALESALRSVCAALEALSERSEGLARCAERARTAQALLERWRDDSRADLV
                     RWIEVFSHSLRLNATPLSIAAIFRRQLDGHPRAWIFTSATLAVAGDFSHYRSEMGLDN
                     ARTAFWDSPFDYPAQALLYVPTKLPDPNSEEHTRAVVASSLPVIRASGGSAFLLFTTL
                     RAMRLAHELLREEFRRHALEFPLMLQGEGSRSELLERFRRLGNAVLLGSASFWEGVDV
                     RGDALSLVVIDKLPFAPPDDPVLAARLDSLAREGGNPFLGYQLPQAVISLKQGAGRLI
                     RDETDRGVLIICDPRLFSKPYGRRILASLPPMKLVRELDKVLSFIAAGGKSLRAAPAA
                     GGSRSEVSDPAG"
BASE COUNT          519 a          902 c          896 g          457 t
ORIGIN      
        1 cgagaaggcc gctcgtgtcc ttgtcgagcc ggtgcacgat tccggcccgc ggaatcgcgg
       61 caagctgcgg caggcggtgc aagagcgcat tgagcagtgt ccctttccag ttgccgcttc
      121 cgggatgaac caccaggccc gccggcttgt tgatcaccag gacgtcgtcg tcttcgtaca
      181 cgacatcgag cgcgatgggc tcggcgcgga acgccgtctc cgccgggccg ggctgcgacc
      241 gtatttcgac gcgctcgccg ccccaaatct tctgtttggg cttggcggca tgcgaatcga
      301 ggctgacgtg cccgtctttc agccacctct gcaggcggtt gcgcgagtgc tcgggaaaca
      361 tccgcgcgag cgccttgtcc aagcgcaagc cggcaagagt atctggtacg cgtcgcgtaa
      421 ccctggccac ggtataattt gccgtctcat ccgcaagctc caccatgaaa cgtagtgtag
      481 ctttattcgc tcttctattg gcggcctgtg ccttcgtcga caagcacgac ccccagtccg
      541 gatggacggc cgagaagctc taccgcgagg cgaaagacgc gctcgacagc ggccagtacg
      601 acctggcgat caagcgctac gaaattctgg aggcacgctt tccctatggg cggtactccc
      661 agcagggtca gctcgagatc gcttacgcct actacaagca gaacgagccg gcatccgcgg
      721 ttgctgcgac agagcggttc gtgaagctgc atcccaacag tcccaacgtc gactacgcgc
      781 attacctgaa aggtctggcc tacttcaacg aagacctggg tattctcggc aggatggcgc
      841 ggcaggaccg gaccgagcgc gatcccaagt ccgcgcggga atcattcgac gccttcaagg
      901 agctggttca gcgctacccg gatagccggt acgcgcccga cgcgatggcg cgcatgaaat
      961 acctcgtcaa cgcgcttgcc tcccacgagg ttcacgtcgc gcgctactac atgaagcgcg
     1021 gcgcctacgt cgccgcggcg aaccgcgcgc agtacgcgct gaaaaattac cctcaagctc
     1081 cggcccagga agaggcgctg ctcatcatga tccaggccta cgacgtgctc ggcatgcccg
     1141 agctgcgcga cgacgccgag cggatcctga ggaaaaattt tcccgacagc gcgcagctga
     1201 aaagcctgct cacccagctg ggtcgctgac ttcggaacgg ctgccgcccg ccgcaggagc
     1261 cgcccggagc gatttcccgc cggcggcgat aaacgaaaga actttgtcga gctcacgcac
     1321 gagcttcatc ggcgggaggc tcgcgaggat cctccggccg tacggcttcg aaaagagcct
     1381 aggatcacag atgatgagca cgccccggtc ggtttcgtcg cggatcagcc tgccggctcc
     1441 ctgcttcagg gaaatgaccg cctggggcaa ttggtagccg aggaagggat tgccgccttc
     1501 gcgcgccagc gaatcgagcc gcgccgcgag cacggggtca tcgggcggcg caaacggcag
     1561 cttgtcgatg accaccagcg acagtgcgtc cccgcgcacg tccacacctt cccagaagct
     1621 ggcgcttccg aggaggaccg cgttgcccag acggcggaaa cgctcgagca gctcgctgcg
     1681 agaaccttca ccctggagca tcaggggaaa ttccagcgcg tgccgccgga attcctcgcg
     1741 caacagctcg tgagcaaggc gcatcgcgcg caaagtcgtg aacaggagga aggcgcttcc
     1801 accgctcgcc cggataaccg gcagggatga cgcgacgacc gcgcgcgtgt gctcttcgct
     1861 gttcggatcc gggagtttcg tcggaacgta gagcaaagcc tgggcggggt agtcgaaagg
     1921 gctgtcccag aacgcggtgc gggcgttgtc caggcccatc tcgctgcggt agtggctgaa
     1981 gtcgccggct accgcgagcg tcgcggatgt aaaaatccag gcgcgcgggt ggccgtcgag
     2041 ctgcctgcgg aaaatggctg cgatggagag cggcgtcgca ttcagccgaa gcgagtgact
     2101 gaatacctcg atccagcgga ccaggtcggc gcggctgtcg tcgcgccagc gctcgagcag
     2161 tgcctgcgcg gttcgggcgc gctcggcgca gcgcgcaaga ccttcgctgc gttcggagag
     2221 cgcctcgagg gccgcgcaca ccgagcgtag cgcggattcg agcgcggcga gcgcatccga
     2281 gacttcgcgc tttcgcccga ccgatgccgc ggtgaagcgc gcgccttcgt tgggaagagc
     2341 gaggcgcaga tcccgggccg ccttgtccag cgcggcagtg agccgcaacg gatcgggcat
     2401 gtccttcgct gcggtgagca cttccgcccg aacgtcccgt gtgagctcga tgatctgccc
     2461 cgtggacagg gattcgccga agaagagcgt cgccgtttca gggagctggt gcgcctcgtc
     2521 gaaaatcacg gtattgcaag cgggcaagag ctccgccatc ccctcgtcct tgagcatcac
     2581 atcggcgaag aacaaatgat ggttgacgac gaccagatcc gcggcgagcg cctcgcgccg
     2641 cgcagccatc acgaaacagt cgcgaaatcg cggacacgac tgtcccaggc agttttcacg
     2701 ggtcgaggtc gcaaggttcc acgccggagc ctcctccggc acgtcggcaa gctcgctctt
     2761 gtctccgctg cggg
//
LOCUS       DASPZD010000460         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1220224, whole genome shotgun sequence.
ACCESSION   DASPZD010000460 DASPZD010000000
VERSION     DASPZD010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220224"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VEM33_08870"
     CDS             complement(<1..606)
                     /locus_tag="VEM33_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HYS51981.1"
                     /translation="MNRLAFLLAMIAGALHGPAVAQAQAWPQKPVRVLVGLAPGGNPD
                     TLARMLAAKWGDAFGQQFVVENRPGAGSTIAADLTAKSAPDGYTVLISDSSVITTAPH
                     LFTSLPYDPFRDLQAVSLAVVVPMWLVVHPSVPANSVAELVAVAKSRKEPMPYASSGN
                     GSIHHITMELFKADTGANFTHVPFKGAGHSVPAMIAGDVQLG"
     gene            complement(625..1716)
                     /locus_tag="VEM33_08875"
     CDS             complement(625..1716)
                     /locus_tag="VEM33_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51982.1"
                     /translation="MKPDSPSPREVLLVGSVGLKDSEDVFRTVGPVLGGRMKRIPDGE
                     TGQRTSWVSRLRFVLEGNLSFEDDPQEVAAGGRITHPTEGTRTWKGSGVIARGVPPPP
                     RLRLKAGVRPEDISIGRLGYPEAAIESYTSFCALRDQGVVPGHLRFQVSLPTTAAFLN
                     AHLVYRHHATVEPVYRGRLFREVDEICEAIRHEDLAIQWDVSTEMGQWEGVRHAGFPD
                     VKAGVIERLAMHCNCVPCDVELGVHLCYGSYGGRHWKEPESTANMVEVHNRLIEELDR
                     PLAYIHMPVPVGRNDEAYFAPLLGLKLRRETKLFLGLVHDSDGVEGTRARIAAAARYA
                     SDFGIAAECGFGRRPPETIPHLLRVHSEV"
     gene            complement(1771..>2499)
                     /locus_tag="VEM33_08880"
     CDS             complement(1771..>2499)
                     /locus_tag="VEM33_08880"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HYS51983.1"
                     /translation="AAMVGRVTAQDWRTASQEPVGLAPDPAATRDAIVQVYAARVIGW
                     RGVFGVHTWIAVKPAAAAEYTVYEIIGWRLRSQDTALVVRHRAPDMRWFGSAPELVAE
                     KRGAGVEELIARIDKAARSYPWAGEYTMWPGPNSNTFTAWVLRAVPELEADLPPTAIG
                     KDYSGRKLVGPAPSGRGFQFSLFGLAAFTASAVEGFEVNLLGLSFGINPFDPSLKLPL
                     VGRVGPARAFASKADEGAAHQHAL"
BASE COUNT          429 a          805 c          860 g          405 t
ORIGIN      
        1 cccgagctgc acgtcgccag cgatcatcgc tggaaccgaa tggcccgcgc ccttgaaagg
       61 cacgtgggtg aaattggctc cggtatcggc cttgaagagc tccatcgtga tgtggtggat
      121 ggagccgttg cctgaagagg cgtaaggcat cggctccttt ctcgatttgg cgaccgccac
      181 cagctcggcg accgagttgg cgggcaccga cggatgcacc accagccaca tcgggactac
      241 cactgcgagc gagacggctt gaagatcgcg gaacgggtca tagggcagtg acgtgaagag
      301 gtgcggtgcg gtggtgatca cgctgctgtc gctgatcagg actgtgtagc cgtcgggtgc
      361 tgacttggcg gtaaggtcgg cggcaatcgt gctgcctgcg ccgggccggt tctcgacgac
      421 gaactgctgg ccaaaggcgt cgccccattt tgccgcgagc atgcgcgcga gcgtgtccgg
      481 attgcccccg ggggcgagtc ccacgaggac gcgcacgggt ttttgcggcc aggcctgagc
      541 ttgcgcgacc gccggcccgt gcaacgcgcc ggcgatcatt gcgagcagaa atgcaagacg
      601 gttcatcgcg gacaaaagat cggctcagac ctcggagtgt acgcgcagca ggtgcgggat
      661 ggtctccggc ggccggcggc cgaagccgca ctccgcggcg atgccgaaat cgctcgcgta
      721 tctcgccgcg gcggcgatgc gtgctcgcgt gccctcgact ccgtccgagt cgtgcaccag
      781 cccgaggaag agcttcgtct cgcgacgcag tttcaacccc agaagcgggg cgaaataggc
      841 ctcgtcgttg cgacccaccg gaaccggcat gtgaatatag gcgagaggcc ggtcgagctc
      901 ctcgatgagc cggttgtgga cctctaccat gttggcggtg ctctccggct ccttccagtg
      961 gcggccgccg tagctgccgt agcaaaggtg cacgccgagc tcgacgtcgc acggaacgca
     1021 gttgcagtgc atcgcgaggc gctcgatcac gcccgccttg acatcgggaa agcccgcgtg
     1081 acgcacgcct tcccattgac ccatctcggt cgagacgtcc cactggatgg cgaggtcctc
     1141 gtgccggatc gcctcgcaga tctcgtccac ttcgcggaaa agccgcccgc gatagacggg
     1201 ctccacggtt gcgtggtgcc tgtagacgag gtgtgcgttc agaaacgccg cggtcgtcgg
     1261 cagggagacc tgaaagcgca agtgcccagg gacgacgccc tgatcgcgca aggcgcaaaa
     1321 gctcgtgtac gactcgatcg cagcttcggg ataaccgagc ctgccaatgc tgatatcttc
     1381 cggccgcacg ccggctttga ggcgcaggcg cggaggcggc ggcacgccgc gcgcaatgac
     1441 gcccgagccc ttccaggtgc gcgtgccttc ggtgggatgg gtgatgcgcc cacccgccgc
     1501 gacctcctgc gggtcgtcct cgaacgagag attgccttcc agcacgaagc gcaggcgtga
     1561 cacccacgag gtccgctgtc cggtctcgcc gtcggggatg cgcttcatgc gaccgccgag
     1621 gaccggcccc acggtgcgaa acacatcctc gctgtctttc aggccgacgc tgccgacgag
     1681 caggacttcc cggggggagg gcgaatcggg cttcacgccc tcaattgtaa gggacgcgga
     1741 cgacgcggct tacttagaag gggaaacggg ctaaagagcg tgctggtgcg ctgcgccttc
     1801 gtcggctttc gacgcgaacg cgcgcgccgg gccgacgcgg cctacgagcg gcagcttgag
     1861 cgagggatcg aagggattga tcccgaagct gagtcccagc aggttcactt cgaagccctc
     1921 gaccgcgctc gcggtgaagg cggcgagtcc gaacagcgag aactggaagc cgcgcccgct
     1981 cggggcgggc ccgacgagtt tcctcccgga ataatccttg ccgatcgcgg tcggcggcaa
     2041 atcggcttcg agctcgggca ccgcgcgcag tacccaggcg gtgaaagtgt tggagttcgg
     2101 gccgggccac atggtgtatt cgccggccca ggggtaggaa cgcgcggcct tgtcgatgcg
     2161 cgcgatgagt tcttcgacgc ccgcaccgcg tttttcagcg acgagctcgg gcgccgatcc
     2221 gaaccagcgc atgtcgggtg cgcgatgccg caccacgagc gcggtgtcct gcgaacgcag
     2281 ccgccagccg atgatttcgt aaacggtgta ctcagccgca gcggccggtt tcaccgcgat
     2341 ccaagtgtgg acgccgaaaa ccccccgcca tccgatgacg cgggctgcgt agacctggac
     2401 gatcgcgtcc cgggtggcag cggggtcggg agcgagtccc accggttcct ggctcgcagt
     2461 gcgccaatcc tgcgcggtga cccggccaac catcgcggc
//
LOCUS       DASPZD010000461         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_533597, whole genome shotgun sequence.
ACCESSION   DASPZD010000461 DASPZD010000000
VERSION     DASPZD010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533597"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1119)
                     /locus_tag="VEM33_08885"
     CDS             complement(235..1119)
                     /locus_tag="VEM33_08885"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HYS51984.1"
                     /translation="MARVQARVEAALERFLPSAGIAPQRLHDAMRYAVLGGGKRIRPL
                     LAFAAGEASSGEPVSADPARLEIAACAVECIHAYSLVHDDLPCMDDDVLRRGKPACHV
                     EFDVATALLVGDSLQSLAFQLLAEHRLCDDPAVQVEMVKILAAACGSRGMAGGQAIDL
                     ASVGQPLTVPELEYMHIHKTGALIRASVALGARCSNALAPGQLARLDHYAKCVGLAFQ
                     VVDDVLDKESSTATLGKTVGKDAANNKPTYVSALGLARARALAEELREEAQAALSDLH
                     GGARRLAELADFIVLRKF"
     gene            complement(1133..1375)
                     /locus_tag="VEM33_08890"
     CDS             complement(1133..1375)
                     /locus_tag="VEM33_08890"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HYS51985.1"
                     /translation="MSKPAKTDQPESFEAALAELEKIVASMESGKLTLEQSLAAHKRG
                     LELAQYCQGVLARAQQQVKVLEENTLKNFPGADDDE"
     gene            1599..2702
                     /locus_tag="VEM33_08895"
     CDS             1599..2702
                     /locus_tag="VEM33_08895"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006765494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYS51986.1"
                     /translation="MSDLLSSRRLKPNASQLPVSWYFDPQVFELEQKLLFANSPGYVA
                     HELMVPNPGDYQTLAWMDHGKVLVRNANGIELLSNVCRHRQATLLEGRGNAQNIVCPL
                     HRWTYDMKGELLGAPHFPENPCLNLGRTPLQNWRGMLFAGKRDVARDLAGFASADDFD
                     FSGYMLDRVEILEFEQNWKTFIEVYLEDYHVIPYHPGLGNFVTCDDLKWDIADTYSAQ
                     VVGVKDGFAKPGTPTYAKWHEALTRYMAGKPAKQGAIWFLYYPNVMLEWYPHVLVVST
                     LLPRSPTHTTNVVEFYYPEDIVLFEREFVEAEQAAYRETAIEDDEIARRMDRGRRALW
                     ERGENDAGPYQSPMEDGMMHFHEYLKRGLAGHL"
BASE COUNT          531 a          915 c          902 g          482 t
ORIGIN      
        1 cagaatgaag gtgcgcagct catcggcgag ccgctgcagc ccgcgccggt ccagggcgcg
       61 cagcgccgac ggattgtcga tggttttcag cagctcgtac gcggccggat cgtaatcgct
      121 cgatccgacc tcgggcctgc cgccgggaag atccgctttt ccgatcgcac tcatagaggc
      181 gaattatccc gcaaacaggg acgcttgtcc ggggggaatg caatttttcc gcagctagaa
      241 cttgcgcaac acgatgaaat cggccagctc cgcgagcctt cgggcgccgc cgtgcagatc
      301 cgatagcgcg gcctgcgcct cttcgcgcag ttcctcggca agcgccctcg cacgcgctag
      361 cccgagcgca gagacgtagg tcggtttatt gttcgccgcg tccttgccga cggtcttgcc
      421 gagcgtcgcg gtcgaggact ccttgtccag aacgtcgtcg accacctgga aggcgagccc
      481 gacgcatttc gcgtagtgat cgaggcgcgc gagttgcccc ggcgcgagcg cgttcgagca
      541 acgcgcgccg agcgcgaccg acgcgcggat cagcgcgccg gtcttgtgga tgtgcatgta
      601 ttcgagctcc ggcacggtca ggggctggcc cacgctcgcg agatcgatcg cctgcccgcc
      661 cgccatgccg cggctgccgc acgcggcggc gaggatcttc accatttcga cctgaaccgc
      721 gggatcgtcg cagagacggt gctcggcgag gagctggaac gcgagcgact gcagactgtc
      781 gcccacgaga agcgcggtcg ccacgtcgaa ttcgacgtgg caagccggct tgccgcggcg
      841 aagcacgtcg tcgtccatgc agggcagatc atcgtgcacg agcgagtagg catgaatgca
      901 ctctacggcg caggccgcga tttccaggcg cgccgggtcg gcgctgaccg gttcgccgga
      961 ggaggcctcc cccgcggcaa acgcgagcaa ggggcggatg cgcttgcctc caccgagcac
     1021 tgcatagcgc atggcgtcat gcaggcgctg cggggctatt cctgcggacg ggagaaaacg
     1081 ctcgagcgcc gcttcgaccc gagcctggac tctcgccatc caggcggtga aatcactcgt
     1141 cgtcgtcggc accggggaag tttttcagcg tgttttcttc gagcaccttg acctgctgct
     1201 gcgcgcgggc gagcacgccc tgacagtact gagccaactc gagcccacgc ttgtgcgcgg
     1261 caagggactg ctccagagtc agcttcccgg actccatgct ggccacgatc ttctccagct
     1321 cggccaaggc ggcctcgaag ctctcgggtt gatcggtctt ggcgggtttt gacatgtggg
     1381 agaggccggt cccggagcgc cgttcggttc aggaaacggc aaaatagccc atgaggaaag
     1441 gacggtcaaa tgaagctctg gagcgaaccc cggcgagcgg gggttttccg atggaaagcg
     1501 ggacgccccg cgggaaaaac ccctggtgcc tccccggaaa aaggtctaga atcgcgccga
     1561 ttttacgatt gaccgaccca agggagtcct ccagccgtat gtccgacctg ctcagttcgc
     1621 gaagactcaa acccaacgcc tcgcagcttc ccgtttcctg gtacttcgac ccgcaggtct
     1681 tcgagctgga gcaaaagttg ctcttcgcga acagcccggg ctatgtcgcg cacgagctca
     1741 tggtgccgaa cccgggcgac taccaaaccc tcgcctggat ggaccacggc aaagtgctgg
     1801 tcaggaacgc gaacggcatc gagctcctgt cgaacgtgtg ccgccaccgg caggcgaccc
     1861 tgctcgaagg ccgaggcaac gcgcagaaca tcgtctgccc gctgcaccgg tggacctacg
     1921 acatgaaggg cgagctgctc ggcgcgccgc acttccccga aaatccctgc ctcaacctcg
     1981 gcaggacccc tctgcagaac tggcgcggca tgctgttcgc gggaaaacgc gacgtggcga
     2041 gggacctcgc cggcttcgcg agcgccgatg acttcgattt ctccggctac atgctcgacc
     2101 gcgtggaaat cctggagttc gagcagaact ggaagacctt catcgaggtg tacctcgagg
     2161 attatcacgt gatcccctac catccgggac tggggaattt cgtcacttgc gacgatctca
     2221 agtgggacat cgctgacacc tattcggccc aggtagtggg agtgaaggac gggttcgcga
     2281 agccgggaac gccgacctac gccaaatggc acgaggcgct tacccgctac atggcgggca
     2341 aaccggccaa gcaaggcgcg atctggttcc tctactaccc caacgtcatg ctcgagtggt
     2401 atccgcacgt gctcgtggtg agcacgctgc tgccgcgttc accgacgcac acgaccaacg
     2461 tcgtcgagtt ctactatccg gaggacatcg tcctcttcga gcgcgaattc gtcgaagccg
     2521 agcaagccgc ctacagggaa accgcgatcg aggacgatga gatcgcgagg cgcatggacc
     2581 gcggccggcg agcgctgtgg gagcgcggcg aaaacgacgc cggcccttac cagtcgccga
     2641 tggaagacgg gatgatgcac ttccacgaat atctgaagcg cggattggcc gggcatctct
     2701 agagtttcct ggcgccggcc gcgagtaaga tagcggcatg attctcaccg cgctcgtcgc
     2761 caccgaaatc ctcgcgcagc gtctcgacga ccccgattgg gtcgtgtttg actgccgcca
     2821 cgatctggcc
//
LOCUS       DASPZD010000462         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_151184, whole genome shotgun sequence.
ACCESSION   DASPZD010000462 DASPZD010000000
VERSION     DASPZD010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151184"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VEM33_08900"
     CDS             complement(<1..490)
                     /locus_tag="VEM33_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HYS51987.1"
                     /translation="MKTILRFVAALGSIVVALPATAALNVFACEPEWSALAQELGGDK
                     VSVYSATTALQDPHRIEARPSLIARIRSADLLICAGSELEIGWIPLLLTQSGNSKIQL
                     GSPGYFEASQYAVRLEVPKVIDRALGDIHPGGNPHVHTDPRNIARIAPVLMERMAQLD
                     PAN"
     gene            complement(501..1829)
                     /locus_tag="VEM33_08905"
     CDS             complement(501..1829)
                     /locus_tag="VEM33_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51988.1"
                     /translation="MHRKATMALAVAAALGPPHAATAQTQDELRTLREQVRQLDKRVT
                     EAEQAATQASSRPTGENAMNPAVSVILNGIYSNLSQDPNTFRINGFVPTLGEVGPGVR
                     GLSLGESELALAANIDHNFRGTLIASITPEDTIGVEEGYIQTLSLSYGFTVKAGRFFS
                     GVGYQNQIHAHAWDFTDAPLANKAFLGNQLNEDGIQLKWVAPTSFYWDVGVEAGRGRQ
                     FPAGPAGGRNKNGVGSGNVFTHVGGDFGTGTAWQTGLSYLSTSPQDRTFDDLDSMGAT
                     VTNSFSGTSRLWVLDGILKWAPNYNPTYTNFKLQGEYFRRRENGDLTFNATGTPQTGG
                     FTSAQSGFYLQGVYQFVPMWRVGYRYDRLNAGTTSNGLVDSGALTAGDFPILAKYNPT
                     RHTVMVDWSASEFSRVRLQLAQDKSRSDATDNQVFLQYIVSLGAHGAHKF"
     gene            complement(1924..2262)
                     /locus_tag="VEM33_08910"
     CDS             complement(1924..2262)
                     /locus_tag="VEM33_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS51989.1"
                     /translation="MLPLTSAVPNRTLRHLLLAAALVFAQQAAQLHALSHLKRDAVMA
                     ERGGKCVPPVNHPAEQCIAYHAVDSVLGALAPAIDLPRVNLPAIASAVLSLLLAARIV
                     FDSRAPPALS"
     gene            complement(2312..2671)
                     /gene="dksA"
                     /locus_tag="VEM33_08915"
     CDS             complement(2312..2671)
                     /gene="dksA"
                     /locus_tag="VEM33_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HYS51990.1"
                     /translation="MSAEQLAFFRERLLETQRELLEKADLTSEHLREHELEADPTDQA
                     TIEEEYALELRARDRERKLLKKIDEALRRIEEGSYGYCEETGDPIGIPRLIARPTATL
                     TIEAQSRREQKQKLYGE"
     gene            complement(2788..>3383)
                     /locus_tag="VEM33_08920"
     CDS             complement(2788..>3383)
                     /locus_tag="VEM33_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYS51991.1"
                     /translation="LVDAKHAARQLDEFHEAQEQVGFADRILLSKTDLVSETEQEALR
                     KRLTKMNPRAPIKKVHFGNTPIEDVLDIRGFNLNATLELDPGFLEDSEHEHDEHVSSF
                     AFRSDKPFDSAKLEEFLSGVVQIYGPDMLRYKGVLYMKGNTHRVVFQGVHMLMGGDLG
                     RPWGKDEKRSSTMVFIGKNLPQDLFMKGLEQCLVEKQ"
BASE COUNT          595 a         1065 c         1052 g          671 t
ORIGIN      
        1 cgttcgcggg atcgagctgc gccatgcgct ccatcagtac cggcgcaatc ctcgcgatgt
       61 tgcgcggatc ggtatgcacg tgcggattgc cgccgggatg gatgtcccca agcgcccggt
      121 cgatcacttt cgggacctcc agtctgactg cgtactgcga ggcttcgaag taacccggac
      181 tgccgagctg gatcttgcta ttcccgcttt gcgtgagcaa gagtggaatc cagccgatct
      241 ccagctccga cccggcacag atcaacaggt cggcggagcg gatgcgtgcg atcaggctcg
      301 gtcgcgcttc gatccggtgc ggatcctgca gcgcagtggt ggccgaatac acggacactt
      361 tgtctccccc tagctcctgc gccagggcgc tccattcggg ctcgcaggca aaaacgttga
      421 gcgccgccgt tgccggcaac gcaacaacaa ttgaacctaa cgcagcaaca aatctcagaa
      481 tcgttttcat gttcgtcacc ctagaacttg tgggcaccat gcgcgcccag actgacgatg
      541 tactggagga agacctggtt gtcggtcgca tccgagcgcg atttgtcctg cgccaactgc
      601 aatcggaccc ggctgaactc gctcgcgctc cagtcgacca tcacggtgtg gcgtgtgggg
      661 ttgtacttgg ccagaatcgg gaaatccccc gcagtgagcg ctcccgagtc gaccaggccg
      721 ttgctggtgg tgcccgcgtt cagccggtcg tagcgatagc cgacccgcca catcgggacg
      781 aactgataca cgccctgcag atagaagcca gattgcgcag acgtgaaacc gcctgtctgt
      841 ggtgttcccg tggcattaaa agtgagatcg ccgttctccc ggcggcggaa gtattcgccc
      901 tggagcttga agttggtgta agtcgggttg taattcggcg cccatttcag gatgccgtcc
      961 agcacccaca ggcggctggt gccggaaaag ctgttggtga ccgtggcgcc cattgaatcc
     1021 aggtcgtcga aggtgcgatc ctggggggag gtgctcaggt acgagagtcc ggtctgccag
     1081 gcggtgcccg ttccgaagtc gccgccgacg tgcgtgaaga cgttgcccga gccgacaccg
     1141 ttcttgttgc gcccgcccgc gggacccgca ggaaactggc gtccgcgacc ggcctcgacg
     1201 ccgacgtccc aatagaaaga agtcggagcg acccacttga gctgtatgcc gtcctcgttc
     1261 aactggttgc cgagaaacgc cttgttggca agcggcgcgt cggtgaagtc ccaggcatgc
     1321 gcgtgaatct ggttctggta ccccactccc gagaagaagc gccccgcttt gacggtgaat
     1381 ccatacgaca gggaaagggt ttggatgtac ccctcctcca cgccgatggt gtcctctggc
     1441 gttatagagg cgatcagcgt tccgcggaag ttgtgatcga tgtttgcggc gagggcgagc
     1501 tccgattcac ccaggctcaa gccccgcacg cccggcccga cctcgccgag cgtgggcaca
     1561 aatccgttga tcctgaacgt gttcggatcc tgggaaagat tcgaatagat gccgttcaga
     1621 atcactgaaa cggcggggtt catcgcattc tcgccggtcg ggcggctgga ggcctgagtc
     1681 gccgcctgct ccgcctccgt cacgcgttta tcgagctgcc tgacctgctc gcgcaaagtc
     1741 cggagctcgt cctgagtctg cgcagtcgcg gcgtggggcg ggcccagcgc cgcagcaacg
     1801 gcgagcgcca tggttgcctt tctgtgcatg ttgtctctcc aagttcatgc gatcgcgacg
     1861 cgccgcaagg ccctcgcgaa cacggccgct tcgcgaaaag cgaaacgggc ctaagggaca
     1921 aaactaggag agggcgggag gcgcgcgcga atcgaatacg atccgcgcag cgagcaggag
     1981 agaaagaacg gccgaggcta tcgccggcaa gttgacccgg ggcaggtcga tcgcgggcgc
     2041 aagagccccc agtacgctgt cgactgcgtg gtacgcgatg cactgctcgg cgggatggtt
     2101 gaccggcggg acgcactttc cgccgcgctc ggccatgacc gcgtcgcgct tgaggtgcga
     2161 cagcgcgtgc agttgcgccg cctgctgcgc aaagacgagc gcggcggcga gaagcaggtg
     2221 acgcagagtg cggttcggca cggctgacgt tagaggcagc atcgggcgca agtctactac
     2281 aaggcgggct cgacgcgcgc ccgctcgaat actactcccc gtagagcttc tgcttctgct
     2341 cgcggcgcga ctgcgcctcg atggtaagcg ttgcagtcgg ccgcgcgatc agcctgggga
     2401 tgccgatcgg atcgcccgtc tcctcgcaat aaccgtagct gccttcttcg atgcggcgca
     2461 gggcctcgtc gattttcttg agaagcttcc tctcgcggtc gcgcgcgcga agctccagcg
     2521 cgtactcttc ctcgatcgtc gcctggtcgg tcggatcggc ctccagttcg tgctcgcgca
     2581 gatgctcgga ggtgagatcc gccttctcca gcaattccct ttgcgtctcg agcaggcgct
     2641 cgcggaagaa cgcgagctgt tcggcgctca tgtactgcga cgcgggggca cgcagcaact
     2701 cggcctcggt caagccgttc ttcttcgccg gcttttgctc cgctaccttc tgctcggccg
     2761 gcttcgccgc cttggtcacc agcttcttta ctgcttttcc accaaacatt gttccaatcc
     2821 cttcataaat aggtcctgcg gcagattctt gccgatgaaa accatcgtgc tcgaacgctt
     2881 ttcgtccttg ccccaggggc gacccagatc gcctcccatg agcatgtgca ccccttgaaa
     2941 cacgacgcgg tgcgtgttgc ccttcatgta cagcacccct ttatagcgca gcatatccgg
     3001 tccgtaaatc tgcacgaccc cggacaggaa ctcctcgagc ttcgcgctat cgaacggctt
     3061 gtccgagcgg aacgcgaagg agctgacgtg ctcgtcgtgc tcgtgctcgc tgtcctcgag
     3121 gaacccggga tcgagctcca gggtcgcgtt gaggttgaag ccgcggatgt cgagaacgtc
     3181 ctcgatcggc gtgttcccga aatgcacctt cttaatgggc gcccgaggat tcatcttcgt
     3241 gaggcgcttt ctcaacgctt cctgctcggt ttccgacacg aggtcggtct tcgacaagag
     3301 aatccggtcg gcgaacccga cctgctcctg agcttcgtgg aactcgtcga gctgcctcgc
     3361 ggcgtgcttt gcgtccacca ggg
//
LOCUS       DASPZD010000463         4584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_151475, whole genome shotgun sequence.
ACCESSION   DASPZD010000463 DASPZD010000000
VERSION     DASPZD010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151475"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VEM33_08925"
     CDS             <1..291
                     /locus_tag="VEM33_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYS51992.1"
                     /translation="IRSPSGQEVRAVASGRVVFADWLRGFGNLLIIDHGKGYLTIYGN
                     NESVLKQVGEPVRTGDAVATVGASGGNMESGLYFEIRHEGKAFDPMRWVSLK"
     gene            339..1742
                     /locus_tag="VEM33_08930"
     CDS             339..1742
                     /locus_tag="VEM33_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYS51993.1"
                     /translation="MMTHIRQATLVLVGVLAGVLLSLNFSAVAQREAARFPLPVDELR
                     AFAEVFGAIKSSYVEPVEDKKLITEAISGMLTGLDPHSAYLDQDAFRELQVGTQGQFG
                     GLGIEVGMEEGFVKVVSPIEDTPAFRAGLKPGDFIIKIDDTSVKGMTLNDAVKRMRGK
                     PKTSIRLTILRKGDAKPFEVTLMRDIIRVQSVKSKLIEPGYGWVRVSQFQEHTGENLV
                     KHVEGLYKNGPLKGLILDLRNDPGGLLNGAVGISSAFLPRGVVVVSTDGRTEDAKRKF
                     IAAPEDYLRGNREDYLKNLPAAVKTVPMVVLVNGGSASASEIVAGALQDHKRATVLGT
                     PTFGKGSVQTILPLGNNTAIKLTTARYYTPSGRSIQAKGIVPDVLIEDPTTPTVGRLR
                     EADLEKHLLNDQEREAQPEKSQRTSAKPSVDAEEKPPAPLEFASEKDFQFQEALKLLK
                     GMPLTTAQSTPPAPAKN"
     gene            1833..2588
                     /gene="moeB"
                     /locus_tag="VEM33_08935"
     CDS             1833..2588
                     /gene="moeB"
                     /locus_tag="VEM33_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HYS51994.1"
                     /translation="MNDEQLLRYSRHILLPEIGVEGQEKLLASSALVIGAGGLGSPAA
                     LYLAAAGVGTITLADGDAVDLTNLQRQILHNTQSIGKPKAASGREALAKINPEVKVVP
                     LEQRLQGEELAPLVASSGIVLDCSDNFATRHAVNRACVRHHKPLVSGAAVRFDGQVSV
                     FDLRDELSPCYHCLFPENAELEEVRCAVMGVFAPLTGIIGTVQAAEALKLLAGAGETL
                     KGRLLILDALAMQWRTVKLVKDPACSVCARVTA"
     gene            complement(2592..3182)
                     /gene="slmA"
                     /locus_tag="VEM33_08940"
     CDS             complement(2592..3182)
                     /gene="slmA"
                     /locus_tag="VEM33_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HYS51995.1"
                     /translation="MPAAKPGARRLQILQVLARMLENPKGEKVTTAALAKELDVSEAA
                     LYRHFASKAQMFEGLIEFIEQTLFGLVNKITTEEEDGLKQVQAILASLLSFSEKNPGM
                     TRVLVGDALVNEDERLQVRINQLHDRLEATLKQCLRVAGTQGHDAPQGPELPADPGPQ
                     ANLMLCYVIGRWQLYAKSGFKRMPSELWEKQWPLLL"
     gene            complement(3327..4214)
                     /gene="argB"
                     /locus_tag="VEM33_08945"
     CDS             complement(3327..4214)
                     /gene="argB"
                     /locus_tag="VEM33_08945"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYS51996.1"
                     /translation="MSNAALTPAHKANVLAEALPYIKRFHGKTIVVKYGGNAMTDELL
                     KRSFAHDVVLLKLVGMNPVIVHGGGPQIDEQLARMGKKGEFVQGMRVTDAETMDVVEM
                     VLGGQVNKEVVTLINQAGGKAVGLTGQDGAFVHAKKMLLSDKNNGGGKIDIGQVGEIA
                     SIDPKVIWALEQGGFIPVVAPIGVGGDGQSYNINADLVAGKLAEVLKAEKLVVLTNTP
                     GVLDKNSKLLTGLTPKKIDALVAEGTLSGGMLPKISSALDAARNGVKSVHIIDGRVEH
                     ALLLEILTDEGVGTLIRSR"
     gene            complement(4316..>4584)
                     /locus_tag="VEM33_08950"
     CDS             complement(4316..>4584)
                     /locus_tag="VEM33_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYS51997.1"
                     /translation="LPGVKKEDIKVNIDGDQVSITAESRAERDEKDGERVLHSERYFG
                     TVSRAFRLSQEIDDARASAKYSDGVLELTLPKKATAAAKQLTIQ"
BASE COUNT          822 a         1435 c         1456 g          871 t
ORIGIN      
        1 attcgctcgc cttcggggca ggaggtgcgg gcggtcgcat cggggcgtgt ggtgttcgcc
       61 gactggctgc gcggcttcgg aaacctgctc atcatcgacc acggaaaggg ctatctcacc
      121 atctacggga acaacgaatc ggtgctcaag caggtcgggg aaccggtgcg cacgggggac
      181 gcggtcgcga ccgtaggagc gagcggcggc aacatggaat cgggtctata cttcgagatt
      241 cgacatgaag gaaaggcctt tgatcccatg cgttgggtgt ccttgaagtg accggcagcg
      301 agcccaagct aacggcgcag gacccaggac cgaacatcat gatgacccat atcagacaag
      361 ccaccttagt gctggtcggc gtgctggcag gagtgctgct cagcctgaac ttctccgcag
      421 tggcccagcg cgaggcggcg cgtttcccgc ttccggtcga tgagctgcgc gccttcgccg
      481 aagtgttcgg cgccatcaag tcgagctacg tcgaaccggt ggaggacaag aagctgatca
      541 cggaggcgat cagcggcatg ctgaccgggc tcgatccgca ctcggcttat ctggaccaag
      601 acgcgttccg cgagcttcaa gtgggcacgc agggccaatt cggcggcctc ggcatcgaag
      661 tcggcatgga agaagggttc gtcaaagttg tgtcgcccat cgaggacacg cctgcatttc
      721 gcgccgggct gaagcccggg gatttcatca tcaagatcga cgacacctcg gtcaagggca
      781 tgaccctgaa cgatgcggtc aagcgcatgc gcggcaagcc gaagacgagc atccgcctca
      841 ccatcctgcg caagggcgat gccaagccct tcgaggtcac cctcatgcgc gacatcattc
      901 gcgtgcaaag cgtgaagtcc aagcttattg agcccggcta cggatgggtc cgagtgtccc
      961 agttccagga gcacaccggt gaaaacctcg tgaagcacgt cgaggggctc tacaagaacg
     1021 ggccgctgaa gggcctgatt ctggatttgc gcaacgatcc aggcgggctc ctgaacggag
     1081 cggtggggat ctcttcggcg ttcctgccgc ggggggtggt ggtggtgtcg accgacggcc
     1141 gtaccgaaga cgccaagcgc aagttcatcg ccgcgccgga ggattatctg cggggcaacc
     1201 gcgaggatta tctgaagaac ctgcctgcgg cggtgaagac cgtgccgatg gtcgtgctcg
     1261 tcaacggcgg atcggcctcc gcgtccgaaa tcgtcgcggg agccctgcag gaccacaagc
     1321 gcgcgacggt gctgggcact cccacgttcg gcaaaggctc ggtgcagacg atccttccgc
     1381 tcggcaacaa taccgcgatc aagctgacca cggcccgcta ctacaccccg agcgggcgct
     1441 cgatccaagc caaaggcatc gtgcccgacg tcctgatcga ggatcccacg acgcctaccg
     1501 tgggacgctt gcgtgaagcc gatctggaaa agcatctcct caacgaccag gagcgggaag
     1561 cccagcccga gaaatcgcag cgcaccagtg caaaaccatc ggtcgatgcg gaagaaaaac
     1621 cgccggcgcc gctagagttc gcctctgaaa aggacttcca gttccaggag gcgctcaagc
     1681 tcctgaaggg catgccgctc accaccgcgc aaagcacgcc gcccgcgccc gccaagaatt
     1741 gataaggcgg acgcgaaaag cgcgccgcct tatccccgcg gaatggaact gacgcgaagc
     1801 gcagttcatt ccgcggccaa tcctatgcac ccatgaatga cgaacaactc ctgcggtatt
     1861 cgcgacacat tctgctcccg gagatcggcg tcgaggggca ggagaagttg ctggcctctt
     1921 cggctctggt gataggtgcg ggagggctcg gttcacccgc ggcgttgtat ctcgcggcgg
     1981 cgggcgtcgg gacgatcact cttgccgacg gggacgcggt ggatctcacc aatctgcagc
     2041 gccagatcct gcacaacact cagtcgatcg ggaaacccaa ggctgcgtcc gggcgcgagg
     2101 cgctcgcgaa aatcaatccc gaagtgaagg tcgtgcccct cgagcagcgc ttgcagggcg
     2161 aggagctggc tccgctggtc gcttcgagcg gcatcgtcct cgactgctcg gacaatttcg
     2221 cgacacgcca cgcggtgaat cgcgcatgcg tgcggcatca caagccgctc gtctcaggcg
     2281 ccgctgtgcg cttcgacggc caggtcagcg ttttcgacct gagggacgag ctcagtccct
     2341 gctatcactg cttgttcccg gagaatgcgg agctcgagga agtgcgctgc gcggtcatgg
     2401 gcgtgtttgc gccgctcacc gggatcatcg gtacggtcca ggcggcggag gcgctgaagc
     2461 tgctcgcggg agccggcgag acgctgaagg ggcgcttgct gattctggac gcgctcgcga
     2521 tgcagtggcg aacggtcaaa ctcgtcaaag atccggcctg ctccgtttgc gcgagggtga
     2581 cagcttaagg ttcagagcag caggggccac tgtttctccc acagctccga aggcatccgc
     2641 ttgaaaccgc tcttggcgta cagctgccag cgcccgatca cgtagcagag catcagattg
     2701 gcctgaggac ccggatcggc gggaagctcg ggtccctgcg gtgcgtcgtg tccttgcgtc
     2761 cccgccacgc gcaggcactg cttgagcgtc gcctcgaggc ggtcgtgcag ctggttgatg
     2821 cggacctgaa gccgctcgtc ctcgttcacg agcgcgtcgc cgaccagcac gcgcgtcatg
     2881 cccggattct tctcggagaa gctgagcagc gaggcaagaa tcgcctggac ctgcttcagg
     2941 ccgtcctcct cctccgtagt gatcttgttc acgaggccga acagcgtctg ctcgatgaat
     3001 tcgatcaggc cctcgaacat ctgcgccttg ctcgcgaagt gccggtagag cgcggcttcg
     3061 gacacatcca gctccttggc cagagccgcg gtcgtcactt tctctccctt cgggttctcg
     3121 agcatccgtg ccagcacttg caggatctgc aggcggcggg caccgggctt ggcagcgggc
     3181 atggcatcct ccgggacggt cggctggatg gttattgtat gactccgggt aagtgcttgc
     3241 taccggcttt cgcaccgatt ttcaagctcg ccgcggattg gatccccttt tagcggggag
     3301 tgccccggga ttcgaatccg gcccggtcac ctggagcgga tcagcgtccc gacaccttca
     3361 tcggtgagaa tttccagcag cagggcatgc tcgacgcggc catcgatgat gtgcacgctc
     3421 ttgacgccgt tcctcgcggc gtcgagagcc gagctgatct tgggcagcat gccgcctgag
     3481 agcgttccct cggccaccag cgcgtcgatt tttttcggag tcaggcccgt gagcagcttg
     3541 ctgttcttgt cgagcacgcc cggagtattg gtcagcacga cgagcttttc ggccttcagg
     3601 acctctgcga gtttcccggc aacgaggtcg gcgttgatgt tgtaggactg cccgtcccca
     3661 ccaacgccga tgggcgcgac gaccggtatg aatccccctt gctcgagcgc ccagatcact
     3721 ttcgggtcga tcgaggcgat ttcgccgacc tggccgatgt cgatctttcc gccgccattg
     3781 ttcttgtcgg agagcagcat ctttttcgcg tgaacgaagg ccccgtcctg acccgtgagc
     3841 ccgactgcct tgccgcccgc ctggttgatg agcgtcacga cttccttgtt gacctggccg
     3901 ccgagcacca tctcgaccac gtccatcgtc tcggcgtccg tcactcgcat gccctggacg
     3961 aattcgccct tcttgcccat ccgcgccagc tgctcgtcga tctgcgggcc gcccccgtgc
     4021 acgatcaccg ggttcatgcc gacgagcttc agcagcacga cgtcgtgggc gaagctgcgc
     4081 ttgagcagct cgtcggtcat tgcgttgccg ccgtacttga cgacgatggt cttcccgtga
     4141 aagcgcttga tgtaaggcag agcctcggcg aggacgttgg ccttgtgagc cggagtcagg
     4201 gcggcgttgc tcatcggcat gcgcatcttg agcgccgatt gtacctttga gcgcggagaa
     4261 aaagaaaaaa aaggcggccg gttggccgcc tctgcctatt gatggctctt gggtattact
     4321 ggatcgtcag ctgcttcgcg gcggccgtcg ctttcttggg caacgtcagt tcgagcacgc
     4381 cgtccgagta cttggcgctg gcgcgggcgt cgtcgatttc ctggctcaac cggaaagcgc
     4441 gcgacaccgt gccgaagtag cgctcgctgt gcagcacgcg ttcgccgtct ttttcgtcgc
     4501 gctcggcgcg cgactcggca gtgatcgaca cttgatcgcc atcgatgttg accttgatgt
     4561 cttccttctt gactcccggc agct
//
LOCUS       DASPZD010000464         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_172911, whole genome shotgun sequence.
ACCESSION   DASPZD010000464 DASPZD010000000
VERSION     DASPZD010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172911"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VEM33_08955"
     CDS             complement(<1..662)
                     /locus_tag="VEM33_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HYS51998.1"
                     /translation="MKIDLDDFRVRPGEKLKLKRWPTRVKPYYASEDQYRKRLAEQVE
                     QLNALQSLLYAVDRHALLIIFQAMDAAGKDGVIKHVMSGVNPQGCQVSSFKHPSAEEL
                     DHDFLWRTTRFLPERGRIGIFNRSYYEEVLIVRVHPEILRAEKLPDDLLDSDSIWKER
                     YRSIAGLEDHLHRNGTRVLKFFLHLSKEEQRRRFLARIDEPHKNWKFSLDDVEERKYW
                     KQ"
     gene            836..1717
                     /locus_tag="VEM33_08960"
     CDS             836..1717
                     /locus_tag="VEM33_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYS51999.1"
                     /translation="MKRVFLAALACGLAAASAAWSQAPARPQIATTKVDGTEGVYIFR
                     NGNHQAMFIVTRDGVIATDPVAYGRPTGGATYVAEIRKVTDKPIKYLVYSHHHYDHIA
                     GGQAFKDAGAKIVSHRKAKERLAVLGDPATLLPDETFDDKRTITLGGTALELTYVGLN
                     HSDSSLIMRLPKEKIVFVVDFLPVGSVPGRGMIDFYPLEAEQSIKQVLAMDWERLIPG
                     HPGPGDRLGTKQDAQDQLTFLQDASAAVKVAAREGKCWEPAEKEVKLPKYEKWPGYEN
                     NLQFVTRRYCGLWGRGT"
     gene            1963..>2084
                     /locus_tag="VEM33_08965"
     CDS             1963..>2084
                     /locus_tag="VEM33_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HYS52000.1"
                     /translation="MPTGTVKWFNPSKGFGFIQPDDKSKDVFVHISAVERAGLG"
BASE COUNT          389 a          646 c          663 g          386 t
ORIGIN      
        1 tattgcttcc agtatttcct ctcctcgacg tcgtcgaggc tgaatttcca gttcttgtgc
       61 ggctcgtcga tgcgcgcgag aaaacgccgg cgctgttcct ccttcgaaag atggaggaaa
      121 aacttgagta cgcgcgtgcc gttgcggtgc agatggtcct ccagcccggc gatcgaacga
      181 taccgttcct tccagatcga gtcggagtcg aggagatcgt cgggcagctt ttcggcgcgc
      241 aggatctccg gatgcacgcg cacgatcagc acttcctcgt aatacgagcg gttgaagatg
      301 ccgatgcgcc cgcgctccgg caggaaacgg gtggtgcgcc agaggaagtc gtgatccagc
      361 tcctcggcgc tcgggtgctt gaagctcgac acctggcagc cctgggggtt gacgccggac
      421 atcacgtgct tgatgacgcc gtccttgccg gcggcgtcca tcgcctggaa gatgatgagc
      481 agggcgtggc ggtcgaccgc gtagagcagg ctttgcagcg cgttcagctg ctcgacctgt
      541 tcggcgagtc gcttccggta ttggtcctcc gatgcgtaat aaggcttgac gcgcgtcggc
      601 catctcttga gcttgagctt ctcgccgggg cggacgcgga aatcgtcgag gtcgatcttc
      661 attcggccaa gggtcgggaa acgcgccagg acattgcggc tatgttagca gcgccgcgcg
      721 cgcccgcacg ggacagggcc aatccaagta ccatggcaaa gcccgggacc gagggaataa
      781 tcgcggcccg ccgcttgttt tagtagggca tcgcacaatc acacaaggag gagacatgaa
      841 acgcgtattt cttgcagctc tcgcctgcgg gctggcggcc gcttccgcgg cctggtcgca
      901 ggcgccggca cgcccgcaga tcgcgacgac gaaggtcgac ggcacggaag gcgtatatat
      961 attccgcaac ggcaatcacc aggcgatgtt catcgtcacc cgggacggcg tgatcgccac
     1021 cgacccggtc gcctacgggc gtccgaccgg cggggcgacc tacgtcgccg agatcaggaa
     1081 ggtcaccgac aagccgatca agtacctcgt ctacagccat caccactacg accacatcgc
     1141 cggcggccag gcgttcaagg atgcgggcgc gaaaatcgtc tcccacagga aagcgaagga
     1201 gcgcctcgca gtcctcggag atccggccac gctgctgccc gacgagacgt ttgacgacaa
     1261 gaggaccatc acgctcggcg gcaccgcgct cgagcttact tatgtcggtc tgaaccactc
     1321 ggacagctcg ctcatcatgc gcttgccgaa ggagaagatc gttttcgtcg tggatttcct
     1381 cccggtcgga tcggtgccgg gtcgcggcat gatcgacttc tatccgctcg aggcggagca
     1441 gtcgatcaag caggtgctgg cgatggattg ggagcgtctt atccccggcc atcccggacc
     1501 cggcgaccgg ctggggacca aacaggacgc gcaggaccag ctcacgttcc tgcaggacgc
     1561 ctcggcggcg gtgaaggtcg cggcgcgcga agggaaatgc tgggagccgg cggagaagga
     1621 ggtcaagctg ccgaagtacg aaaagtggcc gggctacgag aacaacctgc aattcgtgac
     1681 gcggcgctac tgcggcctgt gggggcgcgg cacctgaacc ggcgcgtttt cttttcagga
     1741 acgacggccg ggggcgcgcc tcggccgttc ttgtttccgc cgctcgatcc gcagcgacgc
     1801 ttgctattgc tctccgcacc cgccatactg ggagccatcg aattccacct gggtcagtcc
     1861 tcggcatccc ttccggggtg cgccgattct ggtttctccc acgaccaatt tgatgccccg
     1921 cacactttcg cgcggggcga cttcatccat agagagcatt ccatgcctac cggtacagtt
     1981 aaatggttca acccgagcaa gggtttcggc ttcattcagc ctgacgacaa gtccaaggac
     2041 gtattcgtgc acatctcggc ggtggaacgc gccggcctcg gcaa
//
LOCUS       DASPZD010000465         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_755438, whole genome shotgun sequence.
ACCESSION   DASPZD010000465 DASPZD010000000
VERSION     DASPZD010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755438"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VEM33_08970"
     CDS             <1..333
                     /locus_tag="VEM33_08970"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HYS52001.1"
                     /translation="VLSLIPLEVDLGNNRDKLAHFAAYGGLSFWFGMLIEGRGRELRI
                     AAAFALLGVALEFLQGLTGYRTFDTADMIANATGAALGWGLAQTPLKNALEWAERLIA
                     AASRGRGP"
     gene            complement(337..>2367)
                     /locus_tag="VEM33_08975"
     CDS             complement(337..>2367)
                     /locus_tag="VEM33_08975"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HYS52002.1"
                     /translation="LFSDAAHQGQVRQSGDPYISHPIAVAEIVADWHLDAQAVMAALL
                     HDVMEDTETSKQQITERFGTPVAELVDGLSKLDRIEFQSQEDAQAENFRKMLLAMARD
                     VRVILIKLADRLHNMRTLDSVRPETRRRVARETLEIYAPIANRLGLDSLFRELQELAF
                     SSLYPARYKVLAKAVKAARGNRREVVNKILKALKERLQSTEIDATVTGREKHIYSIYN
                     KMHEKHLSFSQVLDIYGFRVVVEDIPGCYLALGALHGLYKPVPGKFKDYIAIPKVNGY
                     QSLHTTLIGPYGMPVEVQIRTQAMNRIADAGVASHWLYKSSDAEINEVQQRTHKWLQS
                     LLDIQHASGDPAEFLEHIKVDLFPDEVYVFTPKGKIMAMPRGATAVDFAYAVHTDIGN
                     RCAAAKINYELVPLRTELKNGDRVEIITAAHEHPNPAWLSYVKTAKARSQIRHFLKTM
                     QYEEAAALGERMLIQAVRALGAKSSEATRERWERLVRDLGAKSRQEVVADIGLGKRLA
                     AIVARKLLSLTEPHTEGKGPAGSIVIHGSEGLAVQFGKCCNPIPGDPILGFIKKGQGL
                     VVHTNDCPTAAKSRGDPEKWLDVDWAPETDKLFDVAIRVTVANQRGVLAKVATEIAEA
                     DSNIDNVSMDEERSLYTTMHFTLQVANRMHLAKVMRALRRIQEVVRIARVKD"
BASE COUNT          346 a          754 c          799 g          468 t
ORIGIN      
        1 gtcctgtcgc tgatcccgct ggaagtggac ctcgggaaca accgcgacaa gcttgcccat
       61 ttcgccgcct acggcggcct ctcgttctgg ttcggaatgc tgatcgaggg ccgcgggcgc
      121 gagcttcgca tcgcggcggc gttcgccctc ctgggagtgg cgctcgagtt cctgcaggga
      181 ttgaccggct accgtacctt cgacaccgcc gacatgatcg ccaacgcgac cggcgcggcg
      241 ctcggctggg ggctcgccca gacgccgctc aagaacgcgc tcgaatgggc ggagcggctg
      301 atcgcggccg cgtcgcgggg ccgcggcccc tagaagctaa tccttcaccc gcgcgatcct
      361 caccacttcc tggatgcgcc tcaaggcgcg catcaccttg gcgaggtgca tgcggttcgc
      421 gacctgcagg gtgaagtgca tggtggtgta gaggctgcgc tcttcgtcca tgctgacgtt
      481 gtcgatattg gagtcggcct cggcgatctc ggtggcgacc ttggcgagca ccccgcgctg
      541 gttggcgacg gtcacgcgga tcgcgacgtc gaacagcttg tcggtttccg gcgcccagtc
      601 cacgtcgagc catttctccg gatcgccgcg cgatttggcc gccgtcgggc agtcgttggt
      661 gtgcacgacg agcccctggc ccttcttgat gaagccgagg atgggatccc cggggatggg
      721 gttgcagcac ttgccgaact gcacggccaa tccctcggac ccgtggatga cgatcgagcc
      781 ggccgggccc tttccttccg tgtggggctc agtcaggctc agcagcttcc tcgcgacgat
      841 cgccgcgagc cgcttgccca ggccgatgtc ggccacgact tcctggcgcg atttcgcccc
      901 gaggtcgcgc accagccttt cccagcgctc ccgcgtcgcc tccgaggatt ttgcgccgag
      961 cgcgcgcacc gcctggatga gcatgcgctc gccgagcgcc gcggcttcct cgtactgcat
     1021 ggtcttcagg aagtgccgga tctgcgagcg cgccttggcg gtcttcacgt aggacagcca
     1081 cgccgggttg ggatgctcgt gagcggcggt gatgatctcg acccggtcgc cgttcttgag
     1141 ctcggttctg agcggcacca gctcgtagtt gattttcgcc gcggcgcagc ggttgccgat
     1201 gtcggtgtgc accgcgtagg cgaaatccac cgcggtcgct ccgcgcggca tcgccatgat
     1261 cttgcccttg ggagtgaaca cgtagacctc gtccggaaag aggtcgactt tgatgtgctc
     1321 gaggaattcg gcgggatcgc ccgaggcgtg ctggatgtcg agcagcgact gcagccattt
     1381 gtgggtgcgc tgctgcactt cgttgatctc cgcgtcggag ctcttgtaca gccagtgcga
     1441 agcgacgccg gcgtcggcga tgcggttcat ggcctgcgtg cggatctgga cttcgacggg
     1501 catgccgtaa ggcccgatca gcgtcgtgtg cagcgactga tagccgttga ccttcgggat
     1561 cgcgatgtag tccttgaatt ttccgggcac cggcttgtac aggccgtgca gggcgccgag
     1621 cgcgagatag cagccgggaa tgtcctcgac gacgacccgg aaaccgtaaa tgtcgagcac
     1681 ctgcgagaac gacaggtgct tctcgtgcat cttgttgtag atcgaataga tgtgcttctc
     1741 gcggccggtg acggtggcgt cgatctcagt cgactgcagg cgctctttga gcgccttgag
     1801 gatcttgttg acgacttcgc ggcgatttcc gcgcgccgcc ttcaccgcct tggcgagcac
     1861 cttgtagcgc gccggataga ggctcgagaa cgcgagctcc tgcagctcgc gaaacaggct
     1921 gtcgaggccg agccggtttg cgatcggcgc gtagatctcc agcgtctcgc gcgcgacgcg
     1981 cctgcgcgtc tccgggcgga ccgaatcgag cgttctcatg ttgtgcagcc ggtcggcgag
     2041 cttgatcagg atgacgcgaa cgtcgcgcgc catggcgagc agcatcttgc ggaagttctc
     2101 cgcctgcgcg tcctcctggg actggaactc gatgcgatcg agcttggaca ggccgtccac
     2161 gagctccgcc accggcgtgc cgaagcgctc ggtgatctgc tgcttggagg tctcggtgtc
     2221 ctccatcacg tcgtgaagga gcgccgccat caccgcctgg gcgtcgaggt gccagtcggc
     2281 gacgatctcc gcgacggcga tcgggtgtga gatgtaggga tcgccgctct gcctgacttg
     2341 tccctggtgc gcggcatcgc tgaacag
//
LOCUS       DASPZD010000466         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1183000, whole genome shotgun sequence.
ACCESSION   DASPZD010000466 DASPZD010000000
VERSION     DASPZD010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183000"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VEM33_08980"
     CDS             complement(<1..485)
                     /locus_tag="VEM33_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS52003.1"
                     /translation="MKENFIDVEGLRIRYFEEGSGPAVLLLHGASLGSSADVWTGNFS
                     DLAARGLRVIAYDQPGFGLSDNPEDLSVAYRTRFVLAFMDAVGLQKARLIGHSQSGRI
                     AVGLALEHGERVPGVVVLATGSLLPPLPGHGKPEAAEGEEGGAAEPTLEDTRRLLEEN
                     L"
     gene            complement(496..1299)
                     /locus_tag="VEM33_08985"
     CDS             complement(496..1299)
                     /locus_tag="VEM33_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYS52004.1"
                     /translation="MPAKIKHIAIVSDQYALEGRFYEAVFGMRSAADKRPERAVTVSD
                     GYVGLNINPRKAGRPAGLDHFGVEVEDAERVCARLRERHPAIEVLKRPGTRPFAGIST
                     HDPAGNLFDLSQKDMENRTSVYVEPPREQPRVISHLALRTLRADEVAEFYIDLFGFER
                     LAKKAGDPNHYLSDGRITLILMPWSILDYEGGGIARPGPDHIGFKVEDLRAVKERLQT
                     VGDDNPHLRPFPLGAGPEGKARLALFARCPCGAHHLADPDGVLLDIAEK"
     gene            complement(1303..2322)
                     /locus_tag="VEM33_08990"
     CDS             complement(1303..2322)
                     /locus_tag="VEM33_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYS52005.1"
                     /translation="MSNSTASSAPLLAALFFFAQAGTALAQAPARPADAAGGEREVVW
                     YTAMNTPDAAPLRKRFLEKYPDVNLTILRQPGEKIRTRILTEMRAGKFFWDVVSFNHL
                     DMDALAQEGLLAAYSSPETGSGFPGGAVDPEGRWAAIYVRQYVIGYNTRSVPQADAPK
                     SWENLLAPRWKEKFAMDESDVEWYAAMLDYLGSEKGPAFMRSLARQNPQFRRGHSLLA
                     KLLIAGDFPLALVHAAEMDEARRAGAPVDWVKTLDPVITSPSQVAVSAKAPHPNGARL
                     LVDLLLSAEGQALIRDRGRVPARNDVARGQAPIPLKEHYVNPKLAREADRHDKEFREI
                     FLRGR"
     gene            complement(2430..>3652)
                     /locus_tag="VEM33_08995"
     CDS             complement(2430..>3652)
                     /locus_tag="VEM33_08995"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HYS52006.1"
                     /translation="TAKKLKELLAHRTSAPATRPAPVAAPPIAERGYGRAAPVPARLI
                     SSAPLTAPGSEREVRHVALELDESALLYEPGDSLGIWPCNNPDEVELLIAILRAKGSE
                     AVTLPDGAVISAREALGRGCDLRAPSDALYALLAREAKDEADRSRLSKLAEDDSRADA
                     LGVHDVLDALLEFPSARPSIVEFVAALGRMQPRLYSIACSQKRHQREVHLTVSVLRYE
                     KNDRGYLGTGSGFLSEHLRPGRALSVFVQRAHAFRLPADLAAQVIMVGPGTGIAPFRA
                     FLQERAAAGAAGRNWLFFGNHRREADFLYRTELEEFAVKRVLSRMDLAFSRDQTSKVY
                     VQHKMLEAAQELWRWLANGAYLYVCGDAQRMAGDVDLALQQIAITQGGMDGAAAKRFL
                     VELAKAGRYQRDVY"
BASE COUNT          569 a         1208 c         1260 g          615 t
ORIGIN      
        1 aagaggttct cctcgagaag cctgcgagta tcttccagag tgggttctgc ggccccgcct
       61 tcctcgccct ccgcggcttc cggcttgccg tgaccgggca ggggtggcag caggcttccg
      121 gtggcgagca cgacgacccc gggcacgcgc tcgccgtgct ccagggccag gcccacggcg
      181 atgcgcccgg actgcgaatg gcctatgaga cgcgctttct gcagtcccac tgcatccatg
      241 aacgcgagga cgaagcgcgt gcgataggcg acggaaaggt cctcggggtt gtcgctcagg
      301 ccgaatccgg gctgatcgta ggcgatcacg cgcaagccgc gcgcggccag gtcggagaaa
      361 ttgcccgtcc agacgtccgc cgaggatccg agcgacgccc cgtgcagcag caggaccgcc
      421 ggcccgcttc cttcctcgaa gtagcgtatg cgcagccctt ccacgtcgat gaaattctcc
      481 ttcattgtcg ttcgactact tctcggcgat gtcgagcaac acgccgtcgg ggtcggcgag
      541 gtgatgagcg ccgcaggggc agcgggcgaa gagcgcgagc cgcgccttcc cttccggtcc
      601 tgcgcccaag gggaaagggc gcagatgcgg attgtcgtcg cccacggttt gcaggcgctc
      661 cttcaccgcc cgcaggtcct ccaccttgaa gccgatgtga tccggaccgg gcctcgcgat
      721 tccgccgccc tcatagtcga ggatgctcca gggcatgagg atcaaggtga tccggccgtc
      781 gctcagatag tggttcgggt cgccggcctt cttggctagc cgctcgaagc cgaacaggtc
      841 gatgtagaac tccgcgacct cgtcggcgcg cagggtgcgc agggcgagat ggctgatgac
      901 gcgcggctgc tcgcgcggcg gctcgacgta gaccgaagtc cggttctcca tgtccttctg
      961 cgacaggtcg aagaggttgc ccgcggggtc gtgggtgctg atgcccgcga aggggcgcgt
     1021 cccggggcgc ttcagaactt cgattgcggg gtggcgctcg cgcagccgcg cgcaaactcg
     1081 ctcggcgtcc tccacctcca cgccgaagtg gtcgagtcct gccgggcggc ccgccttgcg
     1141 cggattgatg ttgagaccca cgtagccgtc gctgacagtc accgcccgct ccggccgctt
     1201 gtcggccgcg ctcctcatgc cgaacaccgc ctcgtagaat cggccctcga gcgcgtactg
     1261 gtcgctgacg atggcgatgt gcttgatctt cgcaggcatg gatcaacggc ctcgcagaaa
     1321 tatctcccgg aattccttgt cgtgcctgtc ggcttcgcgc gcgagcttcg ggttcacata
     1381 gtgctccttc agcggaatcg gggcttgccc tcgcgcgacg tcgttgcgcg cgggcacgcg
     1441 tccgcgatcc cggataagcg cttgaccctc cgcggagagc agcaaatcca cgagcaggcg
     1501 ggcgccgttc ggatgcggcg cctttgcaga caccgcgact tggctcggcg aggtaatgac
     1561 cgggtcgagg gtcttcaccc agtcgacggg cgccccggcg cgcctcgcct cgtccatttc
     1621 ggcggcgtgc accagcgcca gtggaaaatc ccccgcgatc agcagcttgg cgagcaggct
     1681 gtggccgcgg cggaactgcg gattctgccg cgcgagcgag cgcataaagg cagggccctt
     1741 ttcgcttccg agatagtcga gcatggcggc ataccattcc acgtcgcttt cgtccatggc
     1801 gaacttttcc ttccatctcg gcgcgagcag gttctcccag ctcttcgggg cgtccgcctg
     1861 cggcaccgag cgggtgttgt agccgatcac gtactggcgc acataaatag cagcccagcg
     1921 gccctccggg tcgaccgcgc ctccgggaaa tccgctgccc gtctccgggg acgagtaggc
     1981 ggcgagcagg ccttcctgcg cgagggcgtc catgtccagg tggttgaacg aaaccacgtc
     2041 ccagaagaac tttccggcgc gcatctcggt gaggatgcgc gtgcggatct tctcgccggg
     2101 ctggcgcagg atcgtgagat tcacgtcggg atatttctcc aggaagcgct tccggagcgg
     2161 cgccgcgtcg ggggtgttca tcgcggtgta ccagacgact tcgcgttcgc ctccggccgc
     2221 atcggccggg cgcgcaggag cctgcgcgag agccgtgccc gcttgggcga agaaaaaaag
     2281 cgctgcgagc aggggcgcgg agctcgcggt ggaattcgac atggaatgcc agtctagcat
     2341 cggactgcgg accggccgcg ctggagagat tgccgagcga ggcgggagca gcggacggac
     2401 gccgcttcgc cggcccgccg cgaacgccct cagtacacgt cgcgctggta gcgacccgcc
     2461 tttgccagtt ccacgaggaa acgcttggcg gctgcgccgt ccatcccgcc ctgcgtgatg
     2521 gcgatctgct gcagggcgag gtccacgtcg cccgccattc gctgggcgtc gccgcacacg
     2581 tagaggtacg cgccgttcgc gagccagcgc caaagttcct gcgcagcctc gagcatcttg
     2641 tgctgcacgt acaccttgct cgtctggtcg cgggagaacg cgaggtccat acggctgagc
     2701 acgcgcttca ccgcgaactc ttccagctcg gttcgataga gaaaatccgc ttcgcggcgg
     2761 tggttgccga agaacagcca gttacggccg gcggcgcccg cggccgcgcg ttcctggaga
     2821 aaggcgcgga acggggcgat gccggtgccg gggccgacca tgatgacctg ggcggcgagg
     2881 tcggccggca ggcgaaaggc gtgcgctcgc tgcacgaaca ccgatagcgc gcgccctggc
     2941 cgcaggtgct cggagaggaa tccggaaccg gttcccaggt agccgcggtc gttcttctcg
     3001 tagcgcagca cgctgacggt cagatgcact tcgcgctggt gtcgcttttg cgagcaggcg
     3061 atggaataca gccgcggctg catgcgcccg agcgccgcga cgaattcgac tatgctcggg
     3121 cgcgccgacg gaaattcgag cagcgcgtcg agcacgtcgt gaaccccgag cgcgtcggcc
     3181 cgggaatcgt cctcggcaag cttggagagc ctggagcgat cggcctcgtc cttcgcttcg
     3241 cgcgcgagca gcgcgtagag cgcgtcgctc ggtgcgcgca ggtcgcatcc gcggccgagt
     3301 gcctcgcgcg cggagatgac cgcgccgtcc gggagcgtca cggcctcgga ccccttggcg
     3361 cgcaatatcg cgatcagcag ctcgacttca tcgggattgt tgcagggcca aatacccagc
     3421 gagtcgcccg gctcgtacaa cagggccgat tcgtccagct cgagcgcgac gtggcggact
     3481 tcgcgctccg acccgggcgc ggtgaggggc gcactcgata tcaggcgtgc ggggacgggt
     3541 gcagcacggc cgtagccgcg ctcggctatc ggcggcgccg caaccggggc aggtcgagtc
     3601 gcgggagcgc tcgtacggtg cgcaagcagt tccttgagct ttttcgcggt ga
//
LOCUS       DASPZD010000467         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_348498, whole genome shotgun sequence.
ACCESSION   DASPZD010000467 DASPZD010000000
VERSION     DASPZD010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348498"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..951
                     /locus_tag="VEM33_09000"
     CDS             130..951
                     /locus_tag="VEM33_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HYS52007.1"
                     /translation="MKTRIWLAVGAGALLPLSALAQTAPEVTLTRIECGTAAKTTNVA
                     ERFSDTFAYKPDLALTFTFSCYLIKHGDEYMVWDTGFAPGSNPNAPKVGIVERLAEVK
                     VKPEQVKYVGISHFHGDHTGQLSPFANATLLIGKGDWDGITAIPPMQGANEAGFKDWM
                     GANARKVEPLALDKDVFGDGTVRVIRTPGHTPGHSSLVVRLSSGKNFLLTGDAAHFHE
                     NYQSDGVPSFNYDRAQTVASIERIKKIAANLKATVIIQHDLRDIGKLPAFPAAAK"
     gene            1171..2379
                     /gene="soxC"
                     /locus_tag="VEM33_09005"
     CDS             1171..2379
                     /gene="soxC"
                     /locus_tag="VEM33_09005"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HYS52008.1"
                     /translation="MTGYTVARSAAAEPLKDDPWSLGMGAVTPPLQTPSRFEKHVVRT
                     LSNPNNELRNSHARTPHHLLNGTITPNSMHFSINHSGLPDIDPEKHRLVIHGLVRQPL
                     VFTLESLSRYPMESRMAFVECSGNSAPMFSNQPVQATAQALHGLVSCSEWTGVRLSTL
                     LDEAGFDPKAKWVIAEGGDALALHRSVPMKKALDDAMIGLYQNGERLMPGNGYPMRLL
                     LPGYQGNMNVKFLRRLKLTEGPAMSYYEGKIYSQLLPDGKAYRFHFLQEVKSFITHPS
                     FGLKMNGPGYYEISGIAYSGNGRISRVMVSADGGKTWAQAALQEPVHSKAFARFRAPW
                     RWNGGPAVLQSRAWDEAGNAQPTRAEFVAVRGETKKPVTNLLAFPNQHYNSITSWGIG
                     SDGEIKHVYA"
     gene            2366..>2553
                     /locus_tag="VEM33_09010"
     CDS             2366..>2553
                     /locus_tag="VEM33_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HYS52009.1"
                     /translation="MSTLKLVLAFVLALVLGSGAALAQTPRLGKPISEADIKAWDIAV
                     MPDGTGLPPGSGTATQGAR"
BASE COUNT          502 a          841 c          806 g          404 t
ORIGIN      
        1 ctggcttcag cggcgaattg attctactat gcgatatcga ccgccggctc gctccgggcc
       61 cgccggctca tcgacgggat gctcgagcgc gaagcggcta ccattggcca ctcaaggagg
      121 agtcaaacga tgaagaccag aatctggctc gcggtcggcg ccggcgcgct gctcccgctg
      181 tccgcgctgg cgcagacggc acccgaagtc acgctgaccc ggatcgaatg cggcacggcc
      241 gcgaagacca ccaacgtcgc cgagcgcttc agcgacacgt tcgcgtacaa gccggacctc
      301 gcgctgactt tcacgttcag ctgctacctg atcaagcacg gcgacgagta catggtctgg
      361 gacaccggtt tcgcgccggg ctcgaacccc aacgcaccga aggtgggcat cgtcgagcgc
      421 ctcgccgagg tgaaggtgaa gccggagcag gtgaaatacg tcggcatcag ccacttccac
      481 ggcgaccaca ccggccagct ctcgccgttc gcgaatgcga cgctgctcat cggcaagggc
      541 gactgggacg gcatcacggc aatcccgccg atgcagggcg cgaacgaggc gggcttcaag
      601 gactggatgg gcgcgaatgc ccgcaaggtc gagcccctgg cgctcgacaa ggacgttttc
      661 ggcgacggca cggtgagggt gatccgcacg cccggccaca cgcccgggca ctcgagcctc
      721 gtcgtgcgcc tctcgtcggg aaagaatttc ctgctgaccg gcgacgccgc gcacttccac
      781 gagaactacc agtcggacgg cgtgccgtcc ttcaactacg accgcgcgca gacggtcgcg
      841 tcgatcgagc ggatcaagaa gatcgccgcg aacctcaaag ccaccgtgat catccagcac
      901 gacctgcgcg acatcggcaa gctgccggct tttccggctg cagcgaagta gtcgaaacga
      961 gggcggctca gggtcgaaac cagccgctgc ccgccttaaa ttttaggagc agaatatccg
     1021 tccctaggga ctcgctcaag gaggccggcc atggaaacaa cgccaaacat tcgagcaagg
     1081 caggagttgg aaaggctgga ggtggttgca ggaaatggtc ttcttaaccg ccgtgctttc
     1141 ttgcgcggcg gcgcggcatt cgcggcaacc gtgacgggtt atacggtcgc cagatccgcg
     1201 gcagccgagc cgctcaagga cgatccgtgg agcctgggga tgggcgctgt gacgccgcca
     1261 ctgcagacgc cgtcgcgatt cgagaaacac gtcgtgcgga cgctcagcaa ccccaacaac
     1321 gagctgcgca actcgcacgc gcgcacgccg caccatctgc tgaacggcac gatcacgccg
     1381 aacagcatgc acttcagcat caaccactcc ggcctcccgg atatcgaccc ggaaaagcac
     1441 cgcctggtga tccatggcct ggtcaggcag ccgcttgtct ttacgctcga gtcgctgtcg
     1501 cgctacccga tggaatcgcg catggctttc gtcgagtgca gcggcaacag cgccccgatg
     1561 ttctccaacc agccggtcca ggcgacagcg caggcgctgc acggcctggt gtcctgctcc
     1621 gagtggactg gggtgcgact ttccacgctg ctcgacgaag cgggcttcga tccgaaggcg
     1681 aaatgggtca tcgccgaagg cggggacgcc ctggccttgc accgcagcgt gccgatgaaa
     1741 aaagcgctgg acgacgcgat gatcgggctc tatcagaacg gcgagcggct gatgcccggc
     1801 aacggctacc cgatgcgcct gctgctgccc ggctatcaag gcaacatgaa cgtcaagttc
     1861 ctgcgacggt tgaagctcac cgaagggccg gccatgagct attacgaggg taagatctat
     1921 tcacagctcc taccggatgg aaaggcgtac cggttccact tcctgcagga ggttaaatcc
     1981 tttatcaccc acccctcgtt cggactcaag atgaacggac cgggctacta cgagatttcc
     2041 ggcatcgcct attccggcaa cggccgcatc tcacgggtca tggtttcggc cgatggcggg
     2101 aagacgtggg cgcaggcggc gctccaggag ccggtgcact ccaaggcttt tgcccgcttc
     2161 cgcgcgccct ggcgctggaa tggcggtccc gcggtcctgc aaagtcgcgc ctgggacgaa
     2221 gccggcaacg cgcaaccgac tcgcgccgag ttcgtggccg tgcggggcga gaccaagaag
     2281 ccggttacga atcttctcgc gttccccaac cagcactaca acagcatcac gagctggggg
     2341 atcggcagcg atggggagat caaacatgtc tacgcttaag ctggtcctcg cctttgtcct
     2401 cgcgcttgtc ttaggctcgg gcgcggcgct cgcccagacg ccccgactcg gaaaaccgat
     2461 ctccgaggcg gacatcaagg cctgggacat cgccgtgatg ccggacggca ccggcctgcc
     2521 gcccggcagc gggacggcga cccagggcgc ccg
//
LOCUS       DASPZD010000468         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_953652, whole genome shotgun sequence.
ACCESSION   DASPZD010000468 DASPZD010000000
VERSION     DASPZD010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953652"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VEM33_09015"
     CDS             <1..460
                     /locus_tag="VEM33_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52010.1"
                     /translation="REDGHTLAFFNPYYQVSLPSRFYDPALVGITGRPIDVCYEVTPA
                     GDQVRGNGACARSTSNGTILGITFDDPRSVFDGTDRVVDVNSNFIGNETGPEVWYTDP
                     FGRHGRTTPFPGAIRQWIARINNDRGGLQLEGPGIGGERDYSGPRVHAPN"
     gene            complement(491..1729)
                     /locus_tag="VEM33_09020"
     CDS             complement(491..1729)
                     /locus_tag="VEM33_09020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HYS52011.1"
                     /translation="MAFGFGAAVGALIGYLLGRQTQLPAAARARPAPPSLPPPSFKSA
                     PPASPEIKHVVLDEGSANVLNALNNRLAAIGALADLLHGSPLDPERARALVLLHGEVR
                     RAAEITSHFLELAAHPAGAAEPCDAAVVINGVLAERESTFRELGVKVVRAVPGDLPMV
                     ACPMAQLTEMLTKLLDFSLRRLRASQPPRELRLEVIPTGPSIVASLWDSGTPLAVEAE
                     QRLTSPFRFTQSGGGGEVEFALARALAQSAGGNLRLRPRGGGTGAEVVVTLPRSLLGA
                     HTPLSTPAENLPRMRILVVDDDAVNRQAMVLLLEREGHQIVAVENGLEAMERLGDRDT
                     TFDAIVTDLQMPRLGGRAMYEQLVSSRPLVAKRFVFVTGDQARGETRSFLENCGQPSV
                     TKPYDLGDLINAIGSVSRKK"
     gene            1837..1918
                     /locus_tag="VEM33_09025"
     tRNA            1837..1918
                     /locus_tag="VEM33_09025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1871..1873,aa:Leu,seq:gag)
     gene            complement(1956..2222)
                     /locus_tag="VEM33_09030"
     CDS             complement(1956..2222)
                     /locus_tag="VEM33_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HYS52012.1"
                     /translation="MCRNIKTLANFAPPATDDEIRASALQFVRKLSGTTHPSHANEAA
                     FNGAVDDVTTIARRLIDSLTIKAPPRNREEEARKARERATKRFG"
     gene            2363..2797
                     /locus_tag="VEM33_09035"
     CDS             2363..2797
                     /locus_tag="VEM33_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYS52013.1"
                     /translation="MRFIVMVKANKDSEAGILPTEEQLREMGKFNDELAKAGVMLAGE
                     GLQPSSKGVRISFAGSKRTVRDGPFAETKELVAGFWIWQVKSKQEALEWAKRIPFSQG
                     EEVEIRQVFEAEDFGEAFTPELRKQEQQQRAAIAKNKQRAGK"
     gene            2800..3165
                     /locus_tag="VEM33_09040"
     CDS             2800..3165
                     /locus_tag="VEM33_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYS52014.1"
                     /translation="MKFMTIVKTRETAAPPSPALIEKIVKLGEDAAKKGVMVGMGGLG
                     PTALGARARLSNGKITVTDGPFTEAKEIIGGYAIYDVASKKDAIEWTRKFLEAHIGLW
                     PQDIEVEVRQMMDDPSEGC"
BASE COUNT          610 a          983 c         1062 g          528 t
ORIGIN      
        1 gcgcgaggac ggccacacgc ttgcgttctt caatccctat taccaggtgt ccctgccgag
       61 ccggttctac gatccggccc tggtgggaat taccggacgg ccgatcgacg tgtgctacga
      121 agtcacgccg gcaggggatc aggtccgtgg caatggtgca tgcgcgcgat cgacgagcaa
      181 cggcacgatc ctcggcatca cattcgacga tccgcgctcg gtattcgacg ggacggaccg
      241 cgtcgtcgat gtcaattcga acttcatcgg caacgaaact ggacccgagg tctggtacac
      301 cgatccgttc ggcaggcatg gccgtacgac cccattccct ggggcgattc gtcagtggat
      361 cgcgcggatc aataatgatc gggggggact gcagcttgag gggccgggca tcggtgggga
      421 gagagattac agcggtccga gagtacacgc accaaactaa gacgcagcag acgcaacaga
      481 cacagcaggt ctacttcttt cgcgacaccg atccaatcgc gttaatcaaa tccccaagat
      541 cataaggctt cgtcaccgac ggctgaccgc agttttctag gaacgaccgc gtttctcctc
      601 ttgcctgatc tcccgtgacg aacacgaagc gcttcgcgac gagcggccgg ctgctgacga
      661 gctgctcgta catcgcgcgc ccgccgagcc gcggcatctg caggtccgtg acgatggcgt
      721 cgaaggtggt gtcacgatcg ccgagccgct ccatcgcctc gagcccgttc tcgaccgcga
      781 cgatctgatg accctcgcgc tccaggagca gcaccatcgc ctggcggttc acggcatcgt
      841 catcgacgac gaggatgcgc atgcgcggca ggttttccgc cggtgtcgac agcggcgtgt
      901 gcgcgcccag gaggctgcgc ggcaacgtca cgacgacttc cgcgcccgta ccgccgccgc
      961 gcggccgcag gcggaggtta ccaccggccg attgcgccag cgcccgggcg agcgcgaact
     1021 ccacctcgcc accgccgccc gactgcgtga agcgaaacgg cgacgtcagg cgctgctccg
     1081 cctcgacggc gaggggcgtg cccgaatccc atagggatgc cacgatcgat ggtccggtgg
     1141 ggatcacctc gagccgcagc tcgcggggcg gctgcgaggc gcgcaaccgg cgcaacgaga
     1201 aatcgagcag cttggtcagc atctcggtga gctgcgccat ggggcaggcg accatcggca
     1261 ggtccccggg gaccgcgcgt accactttga cgccgagctc gcggaaggtg gactcgcgct
     1321 ccgccagcac gccgttgatc acgaccgccg cgtcgcacgg ctcggcggcg cccgccgggt
     1381 gcgcggcgag ctcgagaaag tggctggtga tttcggcggc gcgccgcacc tcgccatgca
     1441 gcagcactaa cgcgcgggcg cgttcgggat cgagcgggct gccgtgcagc aaatcggcga
     1501 gcgcgccgat ggcggcgagg cggttgttca gggcattgag gacgttggcc gagccttcgt
     1561 cgagtacgac gtgcttgatc tcgggcgagg cgggcggggc ggacttgaag gaaggtggag
     1621 ggagtgaagg aggcgctggg cgagcacgcg ccgctgccgg gagctgcgtc tgccgtccca
     1681 gcagataccc gatcagcgcg cccactgccg cgccaaaccc gaacgccacc catagctcca
     1741 tgccgaaaag ctatgcgacg cgcgagtttg cgcgagcagc cagccgtcac accgggcttg
     1801 cgaccaccgt cacacggtct ctatttttct tcgcccgccc tggtggtgaa actggtagac
     1861 acgctgtctt gagggggcag tgccgcaagg cgtctcggtt cgaatccgag ccagggcatt
     1921 ttcagtcgag gtcccgccgt ctcctcccgt cgatctcagc caaagcgctt cgtcgcacgc
     1981 tcccgagctt tgcgggcttc ctcttcgcgg ttcctcggtg gcgccttgat cgtcagcgaa
     2041 tcgatcagcc ggcgggcgat cgttgtcacg tcgtcgaccg cgccgttgaa ggccgcctcg
     2101 ttcgcgtgcg aaggatgggt cgtcccgctc aacttgcgca cgaactgcag cgccgaagcg
     2161 cggatttcat cgtcagtcgc cggcggggcg aagttggcca gtgtcttgat gtttctgcac
     2221 atatcggatc ctcgcgattg tcacctaaat agggctacca caacgacatt ggaatatgtc
     2281 gatttctggg ggtctcgttc gtcgcttgga tggaagcaga cggatgcacc aggacctaac
     2341 cagaaagcga gacggagggg caatgcgttt catcgtgatg gtgaaggcca acaaggactc
     2401 cgaagcgggc atcctgccga ctgaagagca gctgcgggag atgggcaaat tcaacgacga
     2461 actggccaag gcgggcgtca tgctcgcggg ggaaggactg cagccgagct ccaagggcgt
     2521 ccgcatcagc ttcgcaggca gcaagcggac ggtccgcgat ggcccgttcg ccgaaacgaa
     2581 agagctggtc gccggcttct ggatctggca ggtcaagtcg aagcaggaag cgctcgaatg
     2641 ggcgaagcgg attccctttt ctcagggcga ggaggttgaa atccgccagg tcttcgaggc
     2701 cgaagatttc ggcgaggcgt tcacacctga gctccgtaag caggaacagc agcagcgggc
     2761 cgcgatcgcc aaaaacaagc aacgcgcggg gaaatgacga tgaaattcat gacgattgtg
     2821 aagacgcgcg agactgctgc gccaccgtcg ccggcgctca tcgagaagat cgtgaaattg
     2881 ggcgaggacg cggccaagaa aggcgtcatg gtcggcatgg gtggtctggg gccgacggcc
     2941 ctgggcgcgc gcgcccggct cagcaacggc aagatcaccg ttacggacgg tcctttcacc
     3001 gaagcgaagg agatcatcgg tggttacgcg atctacgacg tcgcttcgaa gaaggacgcg
     3061 atcgagtgga cccggaaatt cctggaagcg cacatcggac tctggcccca ggacatcgaa
     3121 gtcgaagtgc ggcagatgat ggatgacccg tcagaaggct gctgacgcca tcgatgcggt
     3181 ctg
//
LOCUS       DASPZD010000469         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_416707, whole genome shotgun sequence.
ACCESSION   DASPZD010000469 DASPZD010000000
VERSION     DASPZD010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416707"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1420
                     /locus_tag="VEM33_09045"
     CDS             188..1420
                     /locus_tag="VEM33_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HYS52015.1"
                     /translation="MTSQLEATRIRERFERLLAAGIAIFSRHDLEHVLQQVVDAARDV
                     VGARYAALGVLGGDRETLVQFVTSGLDEATRKKIGDLPRGRGLLGHVIREAKPIRTAD
                     INAHPERYGFPAHHPPMKSFLGVPVKGRGGVFGNLYLTEKIDAPEFNAEDEAIAILLA
                     SQAAVAVENARLYGESEQLLGQIRAMQRQRDLFFAMMNHELRNALTGVYGWAERLVKR
                     KSPEMMEQAGREVYEAAERTLVLLNNFLDLMRLDAGKVRPVIRDVDVPAALQHVIAAS
                     EPAAAAKRVQLKSEAEGALHPLRTDPVRLEQILLNLLSNAIRHSPEGAVVAVAVKHVS
                     GAAWFAVTDHGPGVPVALRDRIFEPFERFDPHSGLGTGLGLPVSRRLAEALGGTLTVE
                     ETSGGGATFTLKIPSSTL"
     gene            1455..2141
                     /locus_tag="VEM33_09050"
     CDS             1455..2141
                     /locus_tag="VEM33_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYS52016.1"
                     /translation="MKILVVEDDRKVAGFIEQGLREEGYVVDVAPDGDQGAMLAHVYD
                     YDLLVLDVMLPKKTGLQLAAELRREGRATPILMLTARDTTEDVVRGLDAGADDYLTKP
                     FKFDELLARVRALVRRGGAGRTELLRYGRVELDRLKHKARVDGKSLDLTPKEFHLLQH
                     FLLHPEEVVRRTELLEKVWDMHFDPESNVVDVHVGNLRRKLKQAAGHELIETVRGVGF
                     RLQTPDRAEA"
     gene            2150..>2867
                     /locus_tag="VEM33_09055"
     CDS             2150..>2867
                     /locus_tag="VEM33_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYS52017.1"
                     /translation="MRRTLALRFAATMAVGLLLATGAFYWSASRALQESIPSAVLRND
                     LLLVLVSIVVAGTAATLMGAWHFTSSAVRPVAEITAQATRIEAGTLDQRILTHADTEE
                     YEGLVAVLNRMLERLERGFAAQRRLTADVSHELRTPLTALRGEIEVALRAPRSQREYE
                     HVLRSGLEEIERLTELSEDLLLVTRAEARVVTAQRVATDIEGLIGDRLDKLRRRCEER
                     DLVVERSLRAGQPAVLIDPEL"
BASE COUNT          496 a          894 c          996 g          481 t
ORIGIN      
        1 aagcgccgca ggcctggagc gccaaggcgc acagcacgat ggggaaagag atcctcagaa
       61 acctcatgat cgcctcgcgg tagatggtgt gcatcgttac gcgcgacggc gggcgcgctg
      121 cagccgcttt aacaatgcag ccgccagcgt gaattgtcac tgcgctttcc gtgaagcatt
      181 atgacgcgtg acctcacaac tcgaggcgac ccgcatccgg gagcgattcg agcggctact
      241 ggcggccggc atcgcgatct tctcccggca cgatctcgag cacgtgctgc agcaggtggt
      301 cgacgcggcg cgtgacgtcg tgggcgctcg ctacgccgcc ctcggcgtcc tgggaggcga
      361 ccgcgagacg ctggtgcagt tcgtcacctc cggtctcgac gaagcgactc ggaagaagat
      421 cggcgatctg ccgcgcggcc ggggattgct cgggcacgtg atccgggaag cgaagcccat
      481 ccgcacggcc gacatcaacg cccacccgga gcgctacggc ttccccgcgc atcacccgcc
      541 gatgaagtca ttcctcggcg ttcccgtgaa gggacgcggt ggcgtgttcg gcaatctcta
      601 tctcaccgaa aagatcgacg ctcccgaatt caacgccgaa gacgaggcga tcgcgatcct
      661 gctggcgtcg caagcggcgg tagccgtcga gaatgcgcgg ctctatggcg agtccgagca
      721 gctcctgggt cagattcgcg cgatgcagcg gcagcgggat ctgtttttcg cgatgatgaa
      781 ccacgagctg cgcaacgcgc tgaccggcgt gtacggctgg gccgagcggc tcgtgaaacg
      841 gaaatcgccc gagatgatgg agcaggcggg gcgcgaagtc tatgaagcgg cggaacggac
      901 gctcgtcctg ctcaacaatt ttctggacct gatgcggctc gatgcgggaa aggtgcggcc
      961 cgtgatccga gacgtcgacg tccccgcggc gctgcaacac gtgatcgcgg cgagtgagcc
     1021 cgccgccgcg gccaaacgcg tgcagttgaa atcggaggcg gagggagccc tccatcctct
     1081 gcgcaccgat ccggtgcggc tcgagcaaat cctcctgaac ctcttgtcga atgccatacg
     1141 ccacagcccg gagggggcgg tcgtggccgt ggcggtgaag cacgtctcag gcgcggcgtg
     1201 gttcgccgtc actgatcatg gccccggggt accggtcgcg ctgcgtgacc gcatcttcga
     1261 gccgttcgag cggttcgatc cccattccgg tctggggacg gggctggggc tgcccgtgtc
     1321 gcgccggctg gccgaggcgt tggggggaac gcttacagtg gaagagactt cgggaggcgg
     1381 cgcgaccttc accctgaaga ttccttcatc aaccctttag gaatgattca acgggccgcc
     1441 gctaggatgc cttcatgaag atccttgtcg tggaagacga ccggaaggtc gcgggtttca
     1501 tcgagcaggg gctgcgtgag gaaggctacg tcgtcgacgt ggccccggac ggcgatcagg
     1561 gcgccatgtt ggcgcacgtc tacgactacg acctgctcgt gctcgacgtc atgctcccca
     1621 agaagaccgg gctgcagctc gctgcagagc tgcggcgcga gggacgcgcg acgcccattc
     1681 tgatgttgac ggcgcgtgac accaccgagg acgtggtccg cgggctcgat gccggcgccg
     1741 acgactatct caccaagccg ttcaagttcg atgagctgct cgcccgggtc cgcgcgctcg
     1801 tgcggcgggg cggcgccgga cgcacggagc tgttgcgcta tggtcgcgtg gagctggacc
     1861 ggctgaagca caaggcccgc gtcgacggca agagtctgga cctcacgccc aaagaattcc
     1921 atctcctgca gcatttcctg ttgcacccgg aggaagtcgt gcgccgcacc gagctcttgg
     1981 aaaaggtgtg ggacatgcat ttcgatcccg agagcaatgt cgtggacgtg cacgtcggca
     2041 atctgcgccg caagctcaag caggcggccg gccacgagct gatcgagacg gtgcgcggcg
     2101 tggggttccg cctgcagacg cccgatcgag cggaggcgtg aagagttcgt tgcgccgcac
     2161 gctggcgtta cggttcgcgg cgacgatggc ggtagggctg ctactcgcga ccggcgcctt
     2221 ttactggtcg gcgtcgcgcg cgctgcagga atccattccg tcagccgtac tccgtaatga
     2281 tctgctgctt gtcctggtct cgatcgtcgt cgcaggaacc gctgcaaccc tcatgggcgc
     2341 gtggcatttc acgtcgtccg ctgttcggcc ggtcgcggag attaccgcgc aggccacgcg
     2401 catcgaggcg gggacgctgg atcaacggat tctgacgcat gcggataccg aagaatacga
     2461 gggactggtc gccgtgctga accggatgct cgagcggctg gagcgtgggt ttgcggcgca
     2521 gcggcgcctc acggccgacg tgagtcacga gctgcgcacg ccgctgacgg cgctgcgcgg
     2581 cgagattgaa gtggcgctgc gcgcaccgcg ttcccagcgt gagtacgagc acgtcctgcg
     2641 cagcggactc gaggagatcg agcgcctcac ggaattgagc gaggatctgc tgctggtcac
     2701 aagggccgag gcccgggtgg tgacggcgca gcgcgtcgcg acggacatcg aaggactaat
     2761 tggcgaccgg ctcgacaagt tgcgccgccg ctgcgaggaa cgcgacctcg tcgtcgaacg
     2821 gtcattgcgc gccggacaac ccgcggtcct gatcgatccg gagctcg
//
LOCUS       DASPZD010000470         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_273978, whole genome shotgun sequence.
ACCESSION   DASPZD010000470 DASPZD010000000
VERSION     DASPZD010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273978"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /gene="trpS"
                     /locus_tag="VEM33_09060"
     CDS             complement(<1..749)
                     /gene="trpS"
                     /locus_tag="VEM33_09060"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYS52018.1"
                     /translation="MYEERVVSGMRPTGAMHLGHFHGALKNWVKLQSEYPCLYFVADW
                     HALTTHYETPEVIEESVWEMLIDWLAAGIDAAQATLFIQSRVPEHAELHTLLSMITPL
                     SWLERVPSYKDQQEKLIDRDLSTYGFLGYPLLQAADVLVYRAKYVPVGEDQVPHVEMM
                     REVARRFNHVYGREPGFEDKAKAAAKKLGSRKAKVLMELRTRYQEQGDAEALATARAL
                     LEQQGNLSVADRERLFGYLEGGGRLILVEPEA"
     gene            complement(756..1424)
                     /locus_tag="VEM33_09065"
     CDS             complement(756..1424)
                     /locus_tag="VEM33_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYS52019.1"
                     /translation="MEFNLVQTIAIYALPVIFAITLHEAAHGYVARHFGDNTAFKLGR
                     VSLNPLRHIDMVGTIVIPLLILVVSKLFGGAGILFGWAKPVPVNFHALRRPKRDMLWV
                     AAAGPAANLVMAIGWALLMKAGQLLPQSMFSLPMSLMAEAGITVNVVLMVLNLLPILP
                     LDGGRIAVSLLPNRLAIGYARLEPFGFPILLMLIFIPPHVLGVVMMPMVQASIGLIEA
                     IFQL"
     gene            complement(1472..2299)
                     /locus_tag="VEM33_09070"
     CDS             complement(1472..2299)
                     /locus_tag="VEM33_09070"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HYS52020.1"
                     /translation="MNIDLHCHSTASDGLLKPEELVARAASNGVGTLALTDHDGVSGL
                     AAARAKAESEGIRFVDGVEISVTWEGSTVHIVGLRIDPENPVLLSGLESIREGRATRA
                     EKMGEALAAAGVPDSLAGAKVYAENPSLISRSHFARHLVKTGRAPDVKSVFQRYLVKG
                     KPGFVPHRWASLGDAVSWIRASGGIAVVAHPGRYNFSKPELHAFLAEFRDCGGAGIEV
                     VTGSHSPEQYSEFARIAREFGFSASRGSDYHGKGESRADLGVLPQLPDDLKPVWHDW"
     gene            complement(2309..3175)
                     /locus_tag="VEM33_09075"
     CDS             complement(2309..3175)
                     /locus_tag="VEM33_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS52021.1"
                     /translation="MKPSHSEFVQIRGLRYHCRCWGEERAPKLFMLHGWMDVSASFQF
                     LMDALRRDWRVIAPDWRGFGLSEWAKSDGYWFPDYFADLDRLLEHFHPDSPATLVGHS
                     MGGNVAAMYAGIRPERVAKLVNLEGLGLTGTRAEKAPERYARWLAELREKPAFRDYEN
                     FEALAERLRGNNSRLSGEKASFLARHWGRSKPDGRVELASDPAHKLVNPVLYRVEEAE
                     ACWRKVVAPMLWVVGAESKAPEMLKLSSADLAARKACFRQLTERVISDAGHMLHHDQP
                     ERLAAVIEEFLG"
BASE COUNT          593 a         1093 c         1082 g          573 t
ORIGIN      
        1 gcctcgggct cgaccaggat caggcgcccg cctccttcga ggtagccgaa caggcgctca
       61 cggtcggcga ccgagaggtt gccttgctgt tccagcagcg cgcgcgcggt cgccagcgcc
      121 tcggcgtcgc cctgctcctg atacctggtg cgcagttcca tcagcacctt ggccttgcgg
      181 ctgcccaatt ttttcgccgc ggccttggcc ttgtcctcga atccgggctc gcggccgtag
      241 acatggttga agcgccgcgc gacctcgcgc atcatttcga cgtgcgggac ctggtcttcg
      301 ccgaccggaa cgtactttgc acggtacacg agcacgtcgg ccgcctgcaa aagcgggtaa
      361 ccgaggaaac cgtaagtcga cagatcccgg tcgataagct tctcctgctg gtccttgtag
      421 cttggtaccc gctcgagcca gctgagcggc gtgatcatcg aaagcagcgt gtgcagttcg
      481 gcgtgctcag ggacgcgcga ctggatgaac agggtcgcct gcgccgcgtc gatgccggcc
      541 gcgagccagt cgatcagcat ttcccaaacg ctctcttcga tcacctcggg cgtttcatag
      601 tgcgtcgtga gcgcgtgcca gtcggcgacg aagtacaggc acggatactc gctctgcagc
      661 ttcacccagt tcttgagtgc gccgtggaag tgtcccaggt gcatcgcgcc cgtggggcgc
      721 atgccggaga ccacgcgttc ttcgtacatg cgccgctata gctggaagat cgcttcgatc
      781 aggccgatcg aagcctgcac catcggcatc atgaccacgc cgagcacgtg cgggggaatg
      841 aagatgagca tgagcaggat cgggaagccg aagggctcca gccgcgcgta gccaatcgcg
      901 agacggttcg gaagcaggct caccgcgatg cgcccgccgt cgagcggcag gatcggcagc
      961 aggttcaaga ccatgagcac gacgttgacg gtgattccgg cttcggccat gaggctcatc
     1021 ggcaaggaaa acatgctctg cggaagcaac tgcccggcct tcatcagcag agcccagccg
     1081 atcgccatga ccaagttcgc ggcgggcccg gccgcggcga cccacagcat gtcccgcttg
     1141 ggtcggcgca gcgcgtgaaa attcaccggc accggcttcg cccagccgaa caggatcccg
     1201 gcgccgccga agagtttgct caccaccagg atcaggagcg ggatgacgat cgtcccgacc
     1261 atgtcgatgt gacgcagcgg attgaggctg acccgaccga gcttgaaggc ggtgttgtcg
     1321 ccgaagtggc gggcgacgta gccgtgagcg gcctcgtgca aagtgatcgc gaagatgacc
     1381 ggcagggcgt agatcgcgat ggtctggacg aggttgaatt ccatggccgc tcgccgtatt
     1441 ttatcagagc ggtctgaacg ccccgagcgt cctaccagtc atgccagacg ggtttcagat
     1501 catcgggcag ctgaggcagc acgccgagat cggccctgct ctcccctttg ccgtgatagt
     1561 cggagccgcg cgaagcgctg aagccgaatt cgcgcgcgat gcgggcgaat tcggagtatt
     1621 gctccggtga atggcttccg gtcacgacct cgatgccggc gcccccgcag tccctgaact
     1681 ccgcgagaaa cgcgtgcagc tcgggcttgg agaaattgta gcggcccggg tgcgcgacga
     1741 cggcgattcc gccgcttgcg cgtatccagc tcaccgcgtc accgagggag gcccagcgat
     1801 gcgggacgaa gccgggcttg cctttcacga ggtagcgctg gaacacgctc ttcacgtccg
     1861 gcgcgcgtcc cgtcttcacc aagtggcgcg cgaaatgcga gcggctgatg aggctggggt
     1921 tctcggcgta aaccttcgcc cctgcgagac tgtcgggaac accggccgcg gcgagcgctt
     1981 cgcccatttt ttccgcccgc gtcgcgcggc cttcccgtat cgactcgagc ccggagagca
     2041 ggaccgggtt ttccgggtcg atccgcagtc cgacgatgtg gaccgtggac ccttcccaag
     2101 tcacagaaat ctcgacgccg tcgacgaagc gaatgccttc cgactcggct tttgcgcgcg
     2161 ccgcggcgag ccccgacact ccgtcgtggt cggtcaatgc gagagtcccg acgccgttcg
     2221 acgcggcccg cgcgacgagc tcttccggct tgagcaaacc gtccgaagcg gtcgagtgac
     2281 aatgcagatc gatattcaat gaggcatctt acccgagaaa ctcctcgatt accgccgcga
     2341 gccgctcggg ctgatcgtga tgcagcatat gaccggcgtc ggagatcacg cgctcggtca
     2401 attgccggaa gcaggccttg cgcgcggcga gatcggcgct ggaaagcttc agcatctcgg
     2461 gcgctttcga ttcggcgccg accacccaga gcatgggcgc gacgaccttg cgccagcacg
     2521 cctcggcttc ctcgactcgg tagagaaccg ggttgacgag cttgtgcgcg ggatcgctcg
     2581 cgagctcgac gcggccgtcg ggcttcgacc tcccccagtg ccgcgcgagg aacgaggcct
     2641 tctcgccgct caggcgggaa ttgttgccgc gcaggcgctc ggcgagcgct tcgaagttct
     2701 cgtagtcgcg aaacgcgggc ttttcgcgaa gctcggccag ccagcgcgcg tagcgctcgg
     2761 gcgccttctc cgcgcgcgtc ccggtgagcc caagaccttc cagattgacg agcttcgcga
     2821 cgcgctcggg gcgtatgccg gcgtacatcg cggcgacgtt gccgcccatg ctgtggccga
     2881 cgagcgtcgc cggagaatcc gggtggaagt gctcgagcag ccggtccagg tcggcgaaat
     2941 agtccgggaa ccagtaaccg tcgctcttgg cccattcgct caggccgaag ccgcgccagt
     3001 ccggcgcgat cacgcgccag tcgcggcgca atgcatccat caggaactgg aacgaggccg
     3061 acacgtccat ccacccgtgc agcatgaaga gcttcggcgc ccgctcctcg ccccagcagc
     3121 ggcagtggta gcgcaagccg cgaatctgga cgaactctga gtgactcggc ttcatgaagc
     3181 gcgtgatgat agcaggcgcc ggatgcgcgg cgcgcttccg atataatcgg ccgcaaccat
     3241 ggcggtctac tcactcggcg aacgcaaagt cgtgttccac ggcgaagagt ggttcatcgc
     3301 acacaacgcc accgtcatcg gcagcgtgat cctgcacaac c
//
LOCUS       DASPZD010000471         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_440137, whole genome shotgun sequence.
ACCESSION   DASPZD010000471 DASPZD010000000
VERSION     DASPZD010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440137"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1296)
                     /locus_tag="VEM33_09080"
     CDS             complement(4..1296)
                     /locus_tag="VEM33_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HYS52022.1"
                     /translation="MACLAILCASPAFAADSPESAPDVHGQATYIRQYKPSFPAAYSG
                     PHSLDPDGAWSYTLTGTLFFGARLGDATELSFNPEFVQGLAFSELLGTGGFTNGEIQR
                     TTGQQLRGYRARLFARHTWNLGGEIEDKESDLNQVRTRYAAERFVLTAGNLSVLDVFD
                     VLEYSHDPRTQFMNWSSLTYGAWDFPADARGYTWGVAAEYITPRFHVRAGRFLVPVES
                     NGLRLEHSVVRRYGDVAEFELPYRLASKPAVARVLAFRNRVSAGEYDDAIALGRATAA
                     TPDVASVRRAELKEGFGISTQVEISEDVGAYVRAGWSDGKTEAFMFTEIDRSLAAGTL
                     IKGSGWRRPEDTVGIAAYINGLSRSHRDYLAAGGLGFFLGDGRLNYAMEQILEVFYSL
                     AVAKKTYLSLGYQRIANPGYNRDRGPADFFSFLFHAEF"
     gene            complement(1336..2580)
                     /locus_tag="VEM33_09085"
     CDS             complement(1336..2580)
                     /locus_tag="VEM33_09085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HYS52023.1"
                     /translation="MRVTRALLAQASAALSSLLELSRPADEALSLFFRGHRSLGQHDR
                     AFIAETAFAALRRRRSLEAAASSGAPEALLAAALLRIIGLSGRALEGLVEEKLMARIR
                     AARAEPFPAAVRADLPDWLWDRLAAGHGDAEAMRIAQGMLNAAPLDLRVNVARVTRDA
                     VLARLGEDEIAASTTPYSPAGIRLSGKPPVNRHPLFRDGLIEVQDEGSQLLAWLLAPR
                     RGEMVADYCAGAGGKTLAMAMLMRGTGRIYALDVSAKRLAALAPRAARASVSNVHAIV
                     LSGESDTRAKRLAGKLDRVLVDAPCSGFGTLRRNPDLKWRHGPEAVAELAQKQHRILA
                     AAARLVKPGGRLVYATCSILPDENEAVADAFCSAHAEFTPLPCNELLAAQRIPLDTGE
                     RLRLWPHIHGTDGFFAAAFQRR"
     gene            complement(2577..3251)
                     /locus_tag="VEM33_09090"
     CDS             complement(2577..3251)
                     /locus_tag="VEM33_09090"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HYS52024.1"
                     /translation="MRNWFFLLISVAALAQAAPPARVEIDYEVTRNGMALADIEHRFE
                     HTGGAYQLTETWRGRGLFYLRGKIRRESRGAITAQGLQPLEYSDKRTGRDTERATFDW
                     NAKTVTVQYQGNPKTLPLPLHPHDRLAFFYQFAFVPPQAGQVALDVIDGKGISDQLYS
                     VENGERLVTPAGEFDTVKLVRRKENNERAELWLARERDFLPVRLLIVGKDGTRLDQVA
                     TRISSP"
     gene            complement(3223..3837)
                     /gene="purN"
                     /locus_tag="VEM33_09095"
     CDS             complement(3223..3837)
                     /gene="purN"
                     /locus_tag="VEM33_09095"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYS52025.1"
                     /translation="MSVAVLISGRGSNMQALLDAGLPITTVISNEPEAKGLETAAARG
                     VATRIVEHRRFSSREAFDAALACEIDRAAPQLIALAGFMRVLTPGFVARYKERLLNIH
                     PSLLPAFPGLRTHERVLAAGAKVHGCSVHFVTTELDHGPIVIQAAVPVRSEDTAETLA
                     ARVLRQEHVIYPRAVNWFLEDRLVIEGDLVRVKGKDAQLVFSAD"
     gene            complement(3869..>4687)
                     /locus_tag="VEM33_09100"
     CDS             complement(3869..>4687)
                     /locus_tag="VEM33_09100"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HYS52026.1"
                     /translation="TPLTLGQEFSGYVAQLELVSEAIRDALPQLYQLAIGGTAVGTGL
                     NTHPQFADRVCERIARETGLRFVSAPNKFAALAGHEPLVFAHGALKTLAAALNKLAND
                     VRLLASGPRSGLGELRIPENEPGSSIMPGKVNPTQAEALSMLCAQVMGNDVALGVGAS
                     SGQLELNVYKPLIVHAFLQSARLLADGSRSFDEHCARGIEANEARIRELVERSLMLVT
                     ALSPHIGYDRAAEIAKKAHRDGTTLREAALALGYVKADDFDRWVRPEAMIGPGS"
BASE COUNT          825 a         1552 c         1521 g          789 t
ORIGIN      
        1 ccgctagaac tctgcatgga acagaaagct gaagaagtcc gccgggccgc ggtcgcggtt
       61 atatcccggg ttcgcgatgc gctgatatcc gagggaaagg tacgtcttct tcgcgaccgc
      121 caagctgtaa aagacttcga ggatctgctc catcgcgtaa ttcaagcgcc cgtcgccaag
      181 gaagaagccc agcccgcccg cggcgagata atcgcgatgc gaccgcgaaa gcccattgat
      241 gtaagccgcg atccctacgg tgtcttcggg acgcctccat ccgctgcctt taatgagcgt
      301 acccgccgcg agcgaacgat caatctccgt gaacatgaaa gcctcggtct tgccgtcact
      361 ccagcccgcg cgcacgtacg cgccgacatc ctcgctgatc tcgacctgcg tactgatccc
      421 gaacccctcc ttgagctcgg cccggcgcac cgacgccacg tccggcgtag ccgctgtagc
      481 tctgcccaga gcgatcgcgt catcgtattc gcctgcgcta acgcgattgc ggaacgccag
      541 cacgcgcgcg acggcaggct tgctggcaag cctgtacggc aattcgaact cggcgacatc
      601 gccatagcgc cggacgacgc tgtgttccag gcgcagccca ttcgactcca ccggcacgag
      661 gaaacgcccg gcgcgcacgt ggaagcgggg cgtaatgtat tcggcggcaa ccccccacgt
      721 gtagccgcgc gcatcagcag ggaaatccca cgcgccgtat gtgagcgaag accagttcat
      781 gaactgggtg cgcggatcgt ggctgtattc cagcacgtcg aagacgtcta acaccgacag
      841 gtttcccgcg gtgaggacaa agcgctcggc tgcgtagcgt gtcctcacct ggttcagatc
      901 cgactccttg tcctcgatct cgccgccgag gttccaggtg tgacgcgcga aaagccgcgc
      961 ccggtagcct ctcaactgtt gtcctgtcgt gcgctggatc tcgccgttgg tgaagccacc
     1021 cgttcccagc aactccgaaa acgccagtcc ctgaacgaac tccggattga aagagagctc
     1081 ggtcgcgtcg ccaagccgtg cgccgaagaa aagcgtccca gtaagcgtgt agctccatgc
     1141 cccgtcgggg tccaggctgt ggggaccgct gtacgccgcg gggaaggacg gcttgtattg
     1201 ccggatgtag gtagcctgtc catggacgtc cggcgcggac tcgggcgagt cggcagcgaa
     1261 ggccgggctc gcgcagagaa tcgctaggca ggccaagcct cgcattgggc gcgaatatag
     1321 caacttcccg cgttgtcagc gtcgctggaa ggctgcggcg aagaagccgt cggtgccgtg
     1381 gatgtgcggc cagagtcgca gccgctcccc ggtgtccagc gggattcgct gagcggcaag
     1441 caattcattg catggaaggg gcgtgaactc ggcatgcgcg gagcaaaatg cgtccgcaac
     1501 agcctcgttt tcatccggca gaatgctgca ggttgcatac acgagccggc cgcctggctt
     1561 caccagccgg gccgcggcgg cgaggatgcg gtgctgcttt tgggcgagct cggcaactgc
     1621 ttcaggtcca tgccgccatt tgaggtccgg attgcgccgc agggtgccga aaccgctgca
     1681 cggcgcgtcg accagcacgc ggtcgagctt gccggcgagg cgcttggcgc gcgtatcgct
     1741 ctcgccggat agcactatcg catgcacatt gctgacgctc gcacgcgcgg cgcgcggcgc
     1801 gagcgcggcg agccgcttcg ccgagacgtc cagcgcatag atgcgcccgg tgccccgcat
     1861 cagcatcgcc atcgcgagcg tctttccacc tgctcccgcg cagtaatcgg ccaccatctc
     1921 gccgcggcgc ggtgcaagca gccaggcgag cagctggctg ccctcgtcct gcacctcgat
     1981 caacccgtcg cggaacagag gatggcggtt gacgggcggc tttcccgaca ggcggatgcc
     2041 tgcgggagaa tacggtgtcg tcgaggctgc aatttcatct tcgcccaggc gagccagcac
     2101 tgcgtcgcgc gtcacgcgcg ctacgttgac acgcaaatcg agcggcgctg cattgagcat
     2161 gccctgtgcg atgcgcatgg cttcggcatc gccgtgaccg gctgccagcc ggtcccatag
     2221 ccagtcgggc agatccgcgc gcaccgccgc gggaaagggc tccgcgcgcg cggcacggat
     2281 ccgcgccatc agcttctcct caacgaggcc ttcgagagcc cgcccggaca ggccgatgat
     2341 gcgcagcagc gctgccgcaa gcagggcctc cggcgcgccg gaactcgcgg ccgcttcgag
     2401 cgagcgcctg cggcgcaatg cggcaaacgc ggtctcggcg atgaatgccc ggtcgtgctg
     2461 accgagactg cggtggccgc ggaagaacag cgaaagcgct tcgtcggcgg ggcgcgaaag
     2521 ctcaagaagg cttgaaagcg cggcgctcgc ctgcgccaga agcgctcgtg taaccctcac
     2581 ggagacgaaa ttcgggtggc gacctggtcg aggcgcgtgc cgtcctttcc gacgatgagc
     2641 agccgtacgg ggagaaaatc gcgctccctc gccagccaga gctcggcgcg ttcattgttt
     2701 tccttgcggc gaacgagctt gacggtgtcg aattcgcccg ccggcgtcac cagccgctcg
     2761 ccgttttcga cgctatatag ctgatcggaa atccctttcc cgtcgatgac atcgagggct
     2821 acctgcccag cttggggcgg aacgaacgca aactggtaaa aaaaagccag ccggtcatgg
     2881 ggatgcagcg gcagcgggag ggttttcggg ttcccctggt attgcacggt gacggtcttt
     2941 gcgttccagt cgaaggtcgc gcgctccgtg tcgcgcccgg tgcgcttgtc cgagtattcc
     3001 agcggctgca aaccctgcgc cgtgatcgcg ccgcggctct cccgcctgat cttcccgcgg
     3061 aggtagaaca ggccacgtcc cctccaggtc tcggtgagct ggtatgcgcc gccggtgtgc
     3121 tcgaagcgat gctcgatgtc ggcaagggcc atgccgttgc gcgtgacctc gtagtcgatc
     3181 tcgacgcgcg ccggcggcgc ggcctgcgcc agcgccgcga cgctaatcag cagaaaaaac
     3241 cagttgcgca tccttcccct taacgcggac caggtcacct tcgatgacca gcctgtcctc
     3301 cagaaaccag ttcacggcgc gcggatagat cacatgctcc tgccgcagca cgcgcgcggc
     3361 gagcgtttcg gccgtgtctt cgctgcgcac cggcaccgcg gcctgaatga cgatcggtcc
     3421 gtgatctagc tccgtcgtga cgaaatggac gctgcagcca tgcaccttcg ccccagcggc
     3481 gagcacacgc tcatgggtgc gcaggccggg gaacgcaggc agcaaggatg gatgaatatt
     3541 gagcagcctc tccttgtagc gcgccacgaa cccgggcgtg agcactcgca tgaatccggc
     3601 cagcgcgatc aattgcggag ccgcgcggtc gatctcgcag gcgagcgcgg cgtcgaatgc
     3661 ctcccggctc gaaaaacggc gatgctcgac gatccgggtc gccacgcccc gcgcggccgc
     3721 ggtttcgagg cccttggctt cgggttcgtt gctgatcacc gtggtgatcg gcagcccggc
     3781 atcgagcaac gcttgcatgt tgctgccccg gcccgaaatg agcaccgcta ctgacatgaa
     3841 tcgcctgaaa gaaaaaagcc gcgtcgactc atgacccggg gccgatcatc gcctcaggac
     3901 gcacccagcg gtcgaaatcg tcggccttga cgtagccgag cgcgagcgcc gcctcgcgca
     3961 atgtggtgcc gtcgcgatgc gccttcttgg cgatttccgc ggcgcggtca taaccgatgt
     4021 gcggtgacag cgccgtgacc agcatcagcg agcgctccac cagctcgcgg atgcgggcct
     4081 cgttggcttc gattccgcgt gcacagtgct cgtcgaaaga acgcgagccg tcggcgagaa
     4141 gccgcgcgct ctgcaggaac gcgtgcacga tcagcggctt gtacacgttc agctcgagct
     4201 gaccggagga cgcgccgaca ccgagcgcga cgtcattgcc catcacctgg gcgcacagca
     4261 tgctgagcgc ttccgcctgc gtaggattga ccttccccgg catgatggag ctgcccggct
     4321 cgttttccgg gatgcgcaac tcgccgagcc cggatcgcgg ccccgaggcg agcagccgca
     4381 cgtcatttgc cagcttgttg agggcggcgg cgagcgtttt cagcgcccca tgggcgaaaa
     4441 ccaggggttc gtgccccgca agcgccgcga atttgttcgg cgcggacacg aagcgcaggc
     4501 ctgtctcgcg cgcaatccgt tcgcagacgc ggtcggcgaa ttgcggatgc gtgttcagcc
     4561 ccgtgccgac ggccgttccg ccgatcgcga gctggtagag ttgcggtaac gcatcgcgga
     4621 tggcctccga taccagctcg agctgggcga cgtagcccga gaactcctgg ccgagcgtga
     4681 ggggggt
//
LOCUS       DASPZD010000472         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_657671, whole genome shotgun sequence.
ACCESSION   DASPZD010000472 DASPZD010000000
VERSION     DASPZD010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657671"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VEM33_09105"
     CDS             <1..370
                     /locus_tag="VEM33_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYS52027.1"
                     /translation="ERAVRERIRNKLIPETQVEMTFERRRPPLEATAASRALGEHALR
                     VYAELGKTLVVDALAEGGGTDAAFASLNTKAPVLERFGLQGFGAHSNDAEYVAVDSIE
                     PRLYLLARLIMDVSQGRDRK"
     gene            452..1279
                     /locus_tag="VEM33_09110"
     CDS             452..1279
                     /locus_tag="VEM33_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYS52028.1"
                     /translation="MSSLASVDGVKLHCEERGGGPAVLFVHEYAGSCRSFDMQVEAFR
                     ARHRCVVFNARGYPPSEVPASVGCYSQYHATADIGAVLDGLAIERAHLVGVSMGAASA
                     LQFALKEPERVVSATLVGIGTGSDDPPLFRLGAEANAKQIESGGMRSFAEQMGRNPNR
                     VRLLEKNPAEFRRSLEELSRMSPVGHANTMRGVQASRPPLYAHEKRLASLRVRTLVVV
                     GDEDAGCRKPSEFLVRVLPDARLVVVPESGHAVNQEEPAEFNRLCLAFIDAATVAGA"
     gene            1413..1955
                     /locus_tag="VEM33_09115"
     CDS             1413..1955
                     /locus_tag="VEM33_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HYS52029.1"
                     /translation="MRLFVFVLLAAASLSAAAGSVDVNLSNDTIEGKLYANAGAADWT
                     FGGMYNRDTKDGTLNLGLLATGDSAFGNSRIEGGVGGKVYSVKAGSGDVIALALGGQL
                     RWFPGNGSFALGAYAFYAPRVVTLLDGDQFFDIGVRAEVEVIRNSFVYIGYRQVQAEL
                     DNNNKVNVDKGAFIGVQIKF"
     gene            complement(2035..2919)
                     /locus_tag="VEM33_09120"
     CDS             complement(2035..2919)
                     /locus_tag="VEM33_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HYS52030.1"
                     /translation="MGLRLKFNLALLFVFALGFAGSGYLSYNLLHKNAREEVLRNAGV
                     MMEAALSMRQYTVSQVRDKLVQKEDEFLPQTVPAFAATEMMNQLRKKYPDYAYKEAAL
                     NPTNPRDRAVGWEADVVNQFRKEVRPEISGTRVTESGLSLYLARPFQIKDQACLSCHT
                     TPEIAPAAMVTRYGRDNGFGWQLNEVIGAQLVSVPMSLPVQNANRAFLTFMASLGGVF
                     VALFVILNLMLYALIVRPVRRVSAAADRLSIGRTSSADKQIPELPESGKDELGVLARS
                     FNRMRRNLEDTIRAMDKR"
     gene            3116..>3709
                     /locus_tag="VEM33_09125"
     CDS             3116..>3709
                     /locus_tag="VEM33_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYS52031.1"
                     /translation="MKVTKSGETLGATIEGLDLARPLSQGEFDEVLRALGRFGVVRFP
                     RQELSGRQLADFSARFGKLEINVANAFQEPGIPEVMILSNIVENGKPIGLADAGQDWH
                     TDMSYSRTIALANVLYGIKIPRRVGKPLGSTEFANMHAAYDGLPAEMRRDLEGMTVLH
                     DFNKFWEMMRKRGSPRSPLSEAQKNAKPPVSHPIFLTH"
BASE COUNT          643 a         1164 c         1245 g          657 t
ORIGIN      
        1 cgagcgggcg gtgcgcgagc gcatccggaa caagcttatc cccgagaccc aggttgagat
       61 gaccttcgaa cggcggcgcc cgccgctcga ggcgacggcg gcttcacggg cgctcggcga
      121 gcatgcactg cgcgtctacg ccgagctcgg aaaaacgctc gtggtcgacg cgctcgccga
      181 aggcggcggc accgacgccg cgttcgcgtc gctgaacacg aaagcgcccg tcctcgagcg
      241 cttcgggttg cagggcttcg gcgcgcactc gaacgacgcc gagtacgtcg cggtcgattc
      301 gatcgagccg cgcctttacc tcctcgcgcg cctgatcatg gacgtgtcgc agggcaggga
      361 ccggaagtga ggcggagcaa ttctccgagc tgatcggcgc aagagtcggg aaatggacag
      421 gggcggtgaa aacttgcggg ctccgcgcgg aatgagctcc ctcgcttcgg tcgacggggt
      481 gaagctccac tgcgaggagc gcggcggggg tccggcggtc ctgttcgtgc acgagtacgc
      541 gggctcctgc cgcagcttcg acatgcaggt cgaggccttt cgcgcccggc atcgttgcgt
      601 ggtcttcaac gcgcgcggtt atccgccctc ggaggtgccg gcgtcggtcg gctgctattc
      661 gcagtaccac gcgaccgccg acatcggcgc ggtcctggac gggctcgcca tcgagcgcgc
      721 gcatctcgtc ggcgtgtcga tgggggcggc ctcggccctg cagttcgcgc tcaaggagcc
      781 ggagcgcgtg gtctcggcga cgctcgtggg catcggcacc ggttcggacg atccgccgct
      841 ttttcgccta ggagcggagg cgaacgcgaa gcagatcgaa tccggcggga tgcggtcgtt
      901 cgccgagcag atgggccgca accccaaccg cgtccgcttg ttggagaaaa atcccgcgga
      961 gttccgccgt tcgctcgagg agctttcaag gatgtcgccg gtcggccacg ccaatacgat
     1021 gcgcggagtg caggcaagcc ggccgccgct ctacgcgcac gagaagcggc tcgcgtcgct
     1081 gcgggtgcgc acgctcgtcg tcgtgggaga cgaggacgcg ggctgccgca agccgagcga
     1141 gtttctcgtg cgcgtgctgc ccgatgcgcg tctcgtcgtc gttccagaga gcggccacgc
     1201 ggtgaaccag gaggaaccgg ccgaattcaa ccgcctttgc ctcgcattca tcgacgcagc
     1261 gacggtcgcg ggagcgtagc tcggtcgatc ggcctcactg tatacgatag agagaaccat
     1321 ggacctgaag cttgccgaac gcacggtggg gcgggaacga atgatgggat aatctgaagc
     1381 tgtcgtcttg gaacttcact ctaaggagcc ttatgcgtct ttttgtgttt gtcttgctcg
     1441 ctgcggcgag tttatcggcc gcggcgggtt ccgtcgacgt caatctttcg aatgacacga
     1501 tcgagggcaa gctctacgcg aacgcaggcg ctgcggactg gaccttcggg ggtatgtaca
     1561 accgcgacac caaggacggc accctgaact tgggcctgct tgcgaccggc gattccgctt
     1621 tcgggaattc gcgcatcgaa ggtggggtgg ggggcaaggt ctactccgtg aaggccggca
     1681 gcggggacgt catcgccctc gcgctcggcg gccagttgcg ctggttcccg ggcaacgggt
     1741 catttgctct cggggcttat gccttctacg caccccgcgt cgtgaccctg ctggacggcg
     1801 accagttttt cgacatcggc gtgcgcgcgg aggtcgaggt gatcaggaac agcttcgtgt
     1861 atatcggcta ccgccaggtc caggccgagc tcgacaacaa caacaaggtg aacgtggata
     1921 agggcgcttt catcggcgtg cagatcaagt tctagtcccg cgtaccggac atgccgtcgg
     1981 cagcgcggct ccccgttccg ttcggagccg cgctcccggc ggtcgcgcgc gaagctatct
     2041 cttgtccatc gccctgatcg tatcttccag gttgcggcgc atgcggttga aggaccgcgc
     2101 cagcaccccc agttcgtcct ttcccgactc gggcagttcc gggatctgtt tgtcggcgct
     2161 gctcgtccgg ccgatgctca gccggtcggc cgcggcggag actctcctca ccggccgcac
     2221 gatgagcgcg tagagcatca ggttcaggat gacgaacagc gcgacgaaaa ccccgccgag
     2281 cgaagccatg aacgtgagga aggcacgatt cgcgttctgg accggaaggg acatcggcac
     2341 cgaaaccagc tgggcgccga tgacttcgtt cagctgccag ccgaagccgt tgtcccgccc
     2401 gtagcgcgtg accattgccg ctggcgcaat ctcgggcgtg gtgtggcaag agagacaagc
     2461 ctgatccttg atctggaacg gcctggcgag atacagcgaa agacccgact ccgtgacccg
     2521 ggttcccgag atctcggggc ggacctcctt gcggaactgg ttgacgacgt ccgcttccca
     2581 gcccacggcg cggtcgcgcg ggttggtcgg attgagcgcc gcttccttgt acgcgtagtc
     2641 gggatacttc ttgcgcagct ggttcatcat ctcggtggcg gcaaacgcgg gcaccgtctg
     2701 gggcaggaac tcgtcctcct tctgcaccag cttgtcgcgc acctggctca ccgtgtactg
     2761 gcgcatcgac aaggccgcct ccatcatcac gcccgcgttg cgcaggactt cctcgcgcgc
     2821 attcttgtgc agcaggttgt aggaaagata gccggagcca gcgaacccca gtgcgaatac
     2881 gaacagcagc gccaagttga acttcagccg aagacccatg gtttttcccc cggtggttgg
     2941 tgtcatgcaa tgccgcgatg ttctgcgggt acgccattat aggtcggctt ccttcgacgg
     3001 cgcatccagg cgcaacgatg acgtgaatta gtcccgcgcg ccggaacggt gtgccccgcc
     3061 gaagctgccc gagacagggt tggcgtaaaa tcgaccgctg cagaggaggt caaggatgaa
     3121 agtgacgaag agcggagaga ctctcggcgc gacgatcgag gggctcgatc tcgcgcggcc
     3181 gctctcgcag ggcgagttcg acgaggtgct gcgcgctctc gggcgattcg gcgtggtgcg
     3241 ctttccgcgc caggagctca gcggccggca gctcgccgat ttcagcgcgc gcttcgggaa
     3301 gctggagatc aatgtcgcca acgcctttca ggagcccgga attcccgagg tgatgatcct
     3361 ctccaacatc gtggagaacg gcaagccgat cgggctcgcc gacgccgggc aggactggca
     3421 caccgacatg tcctacagcc gcacgatcgc tctcgcgaac gtgctctacg gcatcaagat
     3481 ccccaggcgc gtcggcaagc cgctcgggtc gacagagttc gcgaacatgc acgccgcgta
     3541 cgacggcctg ccggccgaga tgagacgtga cctcgaaggc atgaccgtgc tgcacgattt
     3601 caacaagttc tgggaaatga tgcgcaagag aggcagtccg cgttcgccgc tcagcgaggc
     3661 gcagaagaac gcgaagccgc cggtgtcgca cccgattttt ctgacgcac
//
LOCUS       DASPZD010000473         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_34955, whole genome shotgun sequence.
ACCESSION   DASPZD010000473 DASPZD010000000
VERSION     DASPZD010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34955"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..556
                     /gene="soxY"
                     /locus_tag="VEM33_09130"
     CDS             92..556
                     /gene="soxY"
                     /locus_tag="VEM33_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HYS52032.1"
                     /translation="MDPRRREVLKTGGGMLALAAAAGILRPGESLAQEWNKAAFETKS
                     VAETVKALGGGASTESKEIQITAPDIAENGAVVPVSVDSRLARTQAIAIMIEKNPNTL
                     SANFEIPDGTDPFVTTRVKMGETSNVYALVKADGKYYHAVKEIKVTIGGCGG"
     gene            560..877
                     /gene="soxZ"
                     /locus_tag="VEM33_09135"
     CDS             560..877
                     /gene="soxZ"
                     /locus_tag="VEM33_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HYS52033.1"
                     /translation="MADPMRIRANMVADKVEVRVLMSHEMETGLRKDASGNVIPAHFI
                     RNVTVTHAGKTVLSAQWGTSVSRNPFLHFRFKGGRPGERVAITWLDNKGEKRTDEAVI
                     AGG"
     gene            920..1762
                     /gene="soxA"
                     /locus_tag="VEM33_09140"
     CDS             920..1762
                     /gene="soxA"
                     /locus_tag="VEM33_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HYS52034.1"
                     /translation="MRIQCILAGMLAVMLPCLAPAQQDTQKEIERYRQMLQESNPAEL
                     YEARGEELWKTPRGPKNASLEQCDLGLGPGVVKGAYVRLPRYFADTDRVQDAESRLVT
                     CMVALQGFTLDEAKRNPFSGTGVPMITTDPFSGVGRRSVMTDLVAWIASESRGMKIAP
                     PMSHPKERLAYQAGEKLFYYRAGPYDFGCVTCHGEEGKRIRLQNLPAMTNAKDAQRAF
                     GSWPGYRVSEGELRSMQWRMWECIRQMRFPELEFASDASIALITYLAKKAEGGTFDAP
                     SLKR"
     gene            1771..2388
                     /gene="soxX"
                     /locus_tag="VEM33_09145"
     CDS             1771..2388
                     /gene="soxX"
                     /locus_tag="VEM33_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003273925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HYS52035.1"
                     /translation="MKPKTILALALCSIWVAGCATRPSDSEAERAVAEMIKTSFKTRG
                     QATIARLNQDDAQALCSKYRGELPKGVSDGIEKTQQETIRYPGSGKLMGDWREGERIA
                     QSGVGRQFTDAPTRPAGGSCYNCHQLSKQELSFGTIGPSLYQFGKQRGASEAIQKYAY
                     GKIYNSEASTACSNMPRFGYNGVLTEEQITHLVALLLDPESPVNK"
     gene            2391..>2856
                     /locus_tag="VEM33_09150"
     CDS             2391..>2856
                     /locus_tag="VEM33_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYS52036.1"
                     /translation="MSRREFLQALAAAAAAGLPLASRPQDETFYDLPRFGNVSLLHFT
                     DCHAQLLPAHFREPSVNLGIATSANRPPHLVGEALLKHFRLRPGSREAHAFTQLDFVR
                     AAKTYGKLGGFAHLATLVKKLRAERGASLLLDGGDTWQGSATALWTRGQDMIE"
BASE COUNT          575 a          894 c          944 g          443 t
ORIGIN      
        1 caggacgcgg cgagaaaata agcccgtctt ccgtcgcatt cgctcggcgg ccacggtaaa
       61 atcccggttt cccgattatc cgaggaagct ggtggacccc aggcggcgcg aagtgttgaa
      121 gactggcggc ggcatgctcg cgctggcggc cgcggcgggg atcctcaggc ccggcgagtc
      181 gctcgctcag gaatggaaca aagcggcgtt cgaaacgaag agcgtcgcgg agaccgtaaa
      241 ggcgctcggc ggcggcgcct cgaccgaaag caaggagatc cagatcaccg cgccggacat
      301 cgccgagaac ggggcggtcg tcccggtttc ggtcgacagc aggctcgcga gaacccaggc
      361 gatcgcgatc atgatcgaga aaaatccgaa taccctgtcg gcgaatttcg agatccccga
      421 cggcaccgat cccttcgtca ccacgcgggt caagatgggg gagacctcca acgtttacgc
      481 gctggtgaag gccgacggga agtactatca cgcggtgaag gaaatcaagg tgaccatcgg
      541 cgggtgcggg gggtaagaca tggcggatcc catgcgcatc cgcgccaaca tggtggccga
      601 caaggtcgaa gtgcgcgtac tgatgagcca cgagatggaa accgggctgc gcaaggacgc
      661 cagcggcaat gtcattccgg ctcatttcat ccgcaacgtc accgtcactc acgcgggcaa
      721 gactgtgctg tcggcgcagt ggggaacctc ggtgtcgcgc aatcccttcc ttcatttccg
      781 cttcaaaggc ggaagacccg gagagagagt ggcgattacc tggctcgaca ataaaggcga
      841 gaagagaacc gacgaggcag tcatcgccgg cggttgagcg ccggcgctcg agtcggcgga
      901 actcacgagg aagaaagtga tgcgtatcca gtgcatcctg gccggaatgc tggcggtgat
      961 gctgccgtgt ctcgcgccgg cgcagcagga cacccagaag gaaatcgagc gctaccggca
     1021 gatgctgcag gaaagcaatc ctgccgagct gtacgaggcg cgcggggaag agctgtggaa
     1081 aaccccgcgc gggccgaaga acgcttcgct cgagcaatgc gatctcggcc tcgggcccgg
     1141 cgtggtcaaa ggcgcctacg tgcggctgcc gcgctacttc gccgacaccg accgcgtgca
     1201 ggatgccgaa tcgcgcctgg tcacctgcat ggtggcgctg caagggttca cgctcgatga
     1261 ggccaagcgc aatccctttt ccggcacggg tgtgcccatg atcacaaccg acccgttttc
     1321 cggcgtgggt cggcggtcgg ttatgaccga cctcgtcgcc tggatcgcgt ccgagtcgcg
     1381 cggcatgaag atcgccccgc cgatgtccca tccgaaagag cgcctggctt atcaagccgg
     1441 cgaaaagctt ttctactacc gcgccggacc gtatgacttc ggctgcgtca cctgccacgg
     1501 cgaagaagga aagcggatcc ggttgcagaa tttgccggcg atgaccaatg ccaaagacgc
     1561 gcaacgcgca ttcggctcct ggcctggata ccgcgtctcg gagggcgagt tgcgatccat
     1621 gcaatggcga atgtgggagt gcatccggca gatgcgcttt ccggaactgg agttcgcttc
     1681 cgatgcgtca atcgcgctga taacgtacct ggcgaagaag gccgaggggg gcaccttcga
     1741 cgccccgtcg ctcaagcgat agggggcgaa atgaagccga aaacgattct cgcgctggcg
     1801 ctctgcagta tctgggtcgc aggatgcgcg acccggccct ccgattcgga ggcggagcgc
     1861 gcggtagccg agatgatcaa gacctcgttc aagacacgcg gccaggcgac gatcgctcgc
     1921 ctgaaccagg atgacgcgca ggcgctttgc agcaagtatc gcggagaatt acccaaaggc
     1981 gtgagcgacg ggatcgaaaa aacgcaacag gagaccatcc gctacccggg ctccggaaag
     2041 ctcatgggtg actggcgcga gggagagcga atcgcgcaaa gcggcgtcgg caggcagttc
     2101 accgacgccc cgacccggcc ggcggggggc agctgctaca actgccacca gctctcgaag
     2161 caggagctgt cgttcggcac catcggtccg agtctctacc agttcggcaa gcagcgtggc
     2221 gcgagcgagg cgatccagaa gtacgcctat ggcaagatct acaattcgga agcgtccacg
     2281 gcctgctcca acatgccgcg cttcggctac aacggcgtgc tgaccgagga gcagatcacg
     2341 cacctcgtgg cgctgctgct cgacccggag tctcccgtca acaagtgacg ctgtcgcgtc
     2401 gcgaattcct gcaggcgctc gccgccgcgg ccgcggccgg tcttccgcta gcttcgcgcc
     2461 cgcaggacga aactttctac gacttgccgc ggttcggcaa cgtatcgctc ctgcacttca
     2521 ccgactgcca cgcccagctg ctgccggccc atttccgcga gccgagcgtc aatctcggca
     2581 tcgctacgag cgcgaaccgc ccgccgcatc tggtcggcga ggcgctcctg aagcatttcc
     2641 gcctccgccc cggaagccgc gaagcgcacg ccttcaccca gctcgacttc gtgcgggcgg
     2701 cgaagacgta cggaaagctc ggcggcttcg cgcaccttgc aacgctcgtg aagaagctgc
     2761 gcgccgagcg gggcgcgagc ctgctgctcg acggcggcga tacatggcag gggtcggcga
     2821 cggcgctgtg gacgcgcggg caggacatga tcgagg
//
LOCUS       DASPZD010000474         8427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1547455, whole genome shotgun sequence.
ACCESSION   DASPZD010000474 DASPZD010000000
VERSION     DASPZD010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547455"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VEM33_09155"
     CDS             complement(<1..671)
                     /locus_tag="VEM33_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HYS52037.1"
                     /translation="MGHKGTVRLAQILANRSKTALEEAVLLTSEELGFRYFMFCGRFS
                     QPRGATHEVRFDNFPVHWHRYCADRGRDLLPGPLHRLALQEVTPLPWRKIAARHGKAF
                     AKAGEFGLVTGVSCSVHGPRGQWSLTSFALARGGSAAERRILATLPDCQLVACAIHYA
                     AARIARRGVDRVATLRALDGARKRILSDRESQCLIQSARGKTTSEIGQALQISERTVA
                     FHLSN"
     gene            800..2518
                     /gene="phaC"
                     /locus_tag="VEM33_09160"
     CDS             800..2518
                     /gene="phaC"
                     /locus_tag="VEM33_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HYS52038.1"
                     /translation="MANPTFSSSEQFTAAAQALLQNFLGSLGVPPDSEFARSLKSVGV
                     DSSKFAALQARYFQLHAALWQSALARETGRDAVPVAPPDRGDRRFSSAEWQRIPWFDY
                     LRQSYLINSRFLSDWIEALETEPRAKERLSFIARQFSDAMSPANFAATNPEALKLALE
                     TGGESLAQGIRLLLEDVHKGRISTTDESVFEVGKNLAVTEGAVIFENELIQLIQYKPV
                     TSTVFKRPLVMFPPCINKYYILDLQPENSLVRYAVEQGHPVFMVSWRNITEELGRLTW
                     DDYIEKGVLKALEVASAISAAEKVNALGFCVGGTMLGAALAVMAAKGENRVESATFLA
                     SMLDFSDTGDIGLFIDEPGVAVREAAIGKGGIMPGRDLAFVFSALRANDLVWSYVISN
                     YLKGKSPEAFDLLYWNADSTNLPGPMYCWYVRNTYLENRLCEPGKTVVLGVPVDLGKI
                     TVPAYVLATREDHIVPWRTAYRTNQLLGGETRFVLGASGHVAGIINPASKNKRSHWIG
                     AKPAADPEEWLADGKEKPGSWWADWSTWIEGFGDERVKARARPGNAKYKPIEQAPGRY
                     VKHRIA"
     gene            2553..3368
                     /locus_tag="VEM33_09165"
     CDS             2553..3368
                     /locus_tag="VEM33_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HYS52039.1"
                     /translation="MTTAAAEKRKATNSGTEPVREVVPPLRQRIAFVSGGMGGIGSAL
                     CRRLARNGARVVAGCLPGYEKKDEWLVRMRGEGLQVHAAEGDVDDYESCADMFYQIGS
                     VIGPVDILVNNAGITRDGVFKRMSPADWHAVINTNLNSVFNVTRQVIEGMMERGWGRI
                     VNISSVNALKGQFGQTNYSAAKAGMHGFSKALAQEVVRKGVTVNTVSPGYVETDMVRA
                     MKPEILQSIVEQIPMGRLAQPEEIAGLVAYLASEEAGYITGANISINGGLHMV"
     gene            3439..4182
                     /gene="phbB"
                     /locus_tag="VEM33_09170"
     CDS             3439..4182
                     /gene="phbB"
                     /locus_tag="VEM33_09170"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HYS52040.1"
                     /translation="MGTRLALITGGMGGLGETISTKMADAGYRVAVTYSASNKTVGQW
                     IADMKARGYDFRAYACNVADFESCKACTEGVTRDLGPVDILVNNAGITRDMTFRKMTK
                     GDWDAVLHTNLDSVFNMTKQVCDAMVDRGWGRVINVSSVNGSKGAFGQTNYAAAKAGM
                     HGFTKSLALEVAKKGVTVNTISPGYIGTKMVTAIPKEILDSKILPQIPLGRLGKPEEI
                     AGLIIYLCSEEAAFVNGANIAINGGQHMA"
     gene            4278..4898
                     /gene="phaR"
                     /locus_tag="VEM33_09175"
     CDS             4278..4898
                     /gene="phaR"
                     /locus_tag="VEM33_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HYS52041.1"
                     /translation="MSEPARLIKKYPNRRLYDTKTSVYITLADVKKLVMGGEEFQVVD
                     AKTGDDLTRSILLQIILEEEAAGAPMFSSDVLTQFIRSYGNAMQGMLGAYLERNMQLF
                     GEIQKSMREQSQKLYGEPTKGNEELWKQFMSFQGPAMQSLISAYMEQSRTVFQQMQEQ
                     LQNQTRNMFSGFAFPGFPGGPGFGAHPAGAQASQGDNKTPGEEKKS"
     gene            4934..6286
                     /gene="rimO"
                     /locus_tag="VEM33_09180"
     CDS             4934..6286
                     /gene="rimO"
                     /locus_tag="VEM33_09180"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HYS52042.1"
                     /translation="MAAAPKVGFVSLGCPKALVDSEGILTRLRAEGYAISPSYDGADL
                     VVVNTCGFIESAMQESLDAIGEALEENGRVIVTGCLGAKGDVVRRTHPKVLAVSGPHA
                     LEEVMTAVHEHLPKPHDPFADLVPPQGIKLTPRHYAYLKISEGCNHRCTFCIIPSLRG
                     DLASRPIGEVMREAENLVKAGVKELLVISQDTSAYGVDLKYRTGFWGGRPLKARMGDL
                     ATALGELGVWVRLHYVYPYPHVDDVIPLMADGKILPYIDVPFQHASRRILKLMKRPAS
                     GEDNLGRIRAWRRICPDITIRSTFIAGFPGETEDEFRELLDFLEEAELDRVGCFAYSP
                     VEGAKANELSGALPDEVREERRMRLMRLQEKISAKRLKRHVGRIIKVLVDEVSAGGAV
                     ARSSADAPEIDGVVRMEEASKLKPGDWAQVKILSSDAHDLRARLVPGKSKRSVAAQAS
                     "
     gene            6302..7486
                     /locus_tag="VEM33_09185"
     CDS             6302..7486
                     /locus_tag="VEM33_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HYS52043.1"
                     /translation="MAQREVVVTSGVRTAIGDYGGSLKDTPPTKLGAIAIKEAVARAK
                     IDPAAVGHVVLGSVIHGEARDMYISRVAAIEAGLPVGTPCLTVNRLCGSGLQAIVSAS
                     QHILLGDTDVVIGAGAESMTRAAYFMPSARWGQRMGDAQLIDAMTGALHDPFGSGHMG
                     VTAENIAARYGVTRDEQDAFSLESHKRAARATDAGWFKSQIVPVEVKSKKGPAQFDTD
                     EHVRKDLKIEDLQKLKTVFKKDGTVTAGNASGINDAAAAVVLMEKSAAQRQGAKPLAR
                     LVAYAHAGVEPQLMGLGPIPAVRRVFEKAGLKPADMDVIESNEAFAVQAMAVTRDLGL
                     DPAKVNPNGGAVALGHPIGATGCILTVKAIYELHRTGKRYALVTMCIGGGQGIAAIFE
                     RM"
     gene            7486..8379
                     /gene="rssA"
                     /locus_tag="VEM33_09190"
     CDS             7486..8379
                     /gene="rssA"
                     /locus_tag="VEM33_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HYS52044.1"
                     /translation="MELRPRRRPKIGFVLGAGSARGWAHIGVLRALTEAGIRPDVIAG
                     CSVGAFVGAAFSAGRLDQLEAWALALDWKRMLKLADFGLRGGLIKGERVNEVFREQFV
                     ECEFSELPIPFAAVATDLHSGQEMWLREGKVSVAIGASIAVPGLFRPVPYDDRYLVDG
                     SVVNPIPVSLCRAMGADIVLAVDLGSDLVGRYVGDDDRAPTRTGPRGFISRVLPRFNR
                     TGERQAEPPALLETLMGSINIMQLRIARSRLAGEPPDVLLTPRLGNLGLLDYHRAQIA
                     ITEGREAVVRMLPAIRSAIPA"
BASE COUNT         1641 a         2571 c         2788 g         1427 t
ORIGIN      
        1 atgtttgaga ggtggaatgc gacggtgcgc tcggaaatct gaagcgcctg gccgatttca
       61 gaagtcgtct tgccgcgcgc cgactggatc aaacattggc tctcgcggtc gctgagaatc
      121 cgtttgcgag cgccgtcgag cgctcgaagc gtcgcaaccc gatcgacgcc gcgccgggca
      181 atgcgcgccg ccgcgtagtg aatggcgcac gcgacgagct ggcaatccgg cagtgttgcg
      241 agaatgcggc gctccgccgc ggagccgcca cgggcgagcg cgaagctggt gaggctccac
      301 tggcctcgag gtccgtggac cgagcaagaa acccctgtga cgagcccgaa ttcgcccgcc
      361 ttggcgaagg ccttgccatg ccgcgccgcg atttttctcc agggcaaagg ggtgacctcc
      421 tgcagggcga gacggtgcag cggaccgggc aggagatccc tgccacggtc ggcgcagtat
      481 cggtgccagt gaacgggaaa attatcgaac cggacttcgt gcgtcgcgcc gcgcggctgg
      541 gagaagcggc cgcagaacat gaagtagcgg aagccgagtt cctcgctggt gaggagcacc
      601 gcttcctcga gggccgtctt cgagcggttg gcaagtatct gcgcaaggcg cacggtcccc
      661 ttgtggccca tgtggtccct ctcccctagt taactcagta tctaccctat cgggtcgtac
      721 tgccttgact tcgtccattg tgcggcgcaa tatcccgtcg ccctctcgga tacttcaagg
      781 ttttcagcga aaaagctgcg tggccaaccc aacgttctct tcgtccgagc aattcacggc
      841 ggccgcccag gccctcttgc agaatttcct cgggtcgctg ggtgtcccgc ccgattccga
      901 attcgcgcgc tcgctgaagt cggtaggcgt tgactcctcc aagttcgccg cccttcaagc
      961 gcgctacttc cagctgcacg ccgcgctgtg gcagtcagcg cttgctcgcg agacggggcg
     1021 cgatgccgtg cccgtcgcac cgcccgatcg cggcgaccgg cgcttttcct ccgcggaatg
     1081 gcagcgaatt ccctggttcg attacttgcg ccagagctac ctcatcaatt cgcgctttct
     1141 atcggactgg atagaggcgc tcgagacgga accgcgcgca aaagagcgcc taagtttcat
     1201 tgcgcgccag ttttccgacg cgatgagccc ggcgaatttc gctgcgacca atccggaggc
     1261 gctcaagctc gcgctcgaga ccgggggcga gagtctcgcc caaggaattc gcctgctcct
     1321 cgaggacgtc cacaaggggc gcatttcgac cacggacgag tcggtgttcg aggtcggcaa
     1381 gaatcttgcg gtcaccgagg gcgcggtgat tttcgagaac gagctgatcc agctcatcca
     1441 atacaagccg gtcacctcga ctgttttcaa gcggcctctc gtgatgtttc cgccgtgcat
     1501 caacaagtat tacatcctgg atcttcagcc agaaaattcc ctggtgcgct acgcggtgga
     1561 gcagggtcac ccggtgttca tggtgtcgtg gcgcaacatc accgaggagc tcggccgtct
     1621 cacctgggac gattacatcg aaaaaggcgt gctcaaggcg ctcgaggtgg caagcgcgat
     1681 cagcgccgcc gaaaaggtca acgcgctcgg tttctgcgtc ggcggcacga tgctcggtgc
     1741 cgcgctcgcg gtcatggccg caaagggcga aaatcgcgtg gagagcgcga cgttcctcgc
     1801 ctcgatgctc gatttttccg acacgggcga catcgggctt tttatcgacg agccgggcgt
     1861 cgcggtgcgc gaggcggcga tcggcaaggg cggcatcatg cccggtcgcg atctcgcgtt
     1921 cgtgttctcg gcgctccgcg cgaacgacct cgtttggtcg tacgtgatca gcaactatct
     1981 gaagggcaag tcgcccgaag ctttcgacct gctctactgg aatgcggatt ccaccaatct
     2041 tcccggaccc atgtactgct ggtacgtgcg caacacctat ctcgaaaacc gcctgtgcga
     2101 gccagggaag acggtggttc tcggagtgcc ggtggatctc gggaagatca ccgttccggc
     2161 ttatgtgctc gccacgcgcg aggaccacat cgtgccttgg cgcacggcct atcggacgaa
     2221 ccagctgctt gggggcgaga cgcgcttcgt tctcggagcg agcgggcacg tcgccggaat
     2281 catcaatccc gcctcaaaaa ataagcgcag ccactggata ggcgcaaagc cggctgctga
     2341 ccccgaggaa tggctcgccg acggaaagga aaaacccgga agctggtggg ccgactggag
     2401 cacttggatc gagggtttcg gagatgagcg agtcaaagcg cgcgcgcggc cgggcaacgc
     2461 gaaatacaaa cccatagagc aggcgcccgg gcgctatgtg aagcaccgga tcgcatgagc
     2521 aaaggacatt tttttcagag agagatcgaa ccatgacgac ggcggcagcg gaaaaacgga
     2581 aagccacgaa ctccggcacg gaaccggtcc gggaagtcgt gcctcccctc aggcagcgca
     2641 tcgcgttcgt gagcggcggc atgggcggaa tcggctccgc actgtgcagg cgtctcgcgc
     2701 gaaacggcgc gagagtcgtt gcgggctgtc ttcccggtta cgaaaagaag gacgagtggc
     2761 ttgtaagaat gcgcggcgaa ggcctccaag tccacgctgc ggagggcgac gtcgacgact
     2821 acgagtcctg cgccgacatg ttctaccaga tcggctcggt gatcggcccg gtggacatcc
     2881 tcgtcaacaa tgcgggcatt acgcgcgacg gcgtgttcaa gcgcatgtcg cccgccgact
     2941 ggcacgctgt aatcaacacc aacctgaaca gcgtgttcaa cgtcacgcgg caggtgatcg
     3001 agggaatgat ggagcggggc tggggacgca tcgtcaacat ctcctcggtc aacgcgctga
     3061 agggccagtt cggtcagacc aactactcgg ctgcgaaggc aggcatgcac ggattctcca
     3121 aggcgctcgc gcaagaggtg gtgagaaaag gcgtcaccgt gaacacggtg tcacccggct
     3181 acgtcgagac ggacatggtg cgggcgatga aaccggagat cctgcaatcc atcgtcgagc
     3241 agattcccat ggggcggctc gcgcaacccg aggagatcgc gggcctcgtc gcttacctcg
     3301 cctcggaaga ggcgggctac atcacgggcg cgaacatctc gatcaacggc ggactgcaca
     3361 tggtttgact ccgatccgaa cggtgcaata atccaatagc acgatcggga cggcaccgag
     3421 tcgacgcagg gaggagtgat gggaacgagg ctggcgctga tcacaggagg catgggcggg
     3481 ctgggggaga cgatcagcac gaagatggcc gatgccggat accgcgtagc cgtaacctat
     3541 tccgcctcga acaagacggt cggccagtgg atcgccgaca tgaaggcgcg tggttacgat
     3601 ttccgcgcgt atgcctgcaa cgtcgcggac ttcgaatcgt gcaaggcgtg cacagaaggc
     3661 gtgacccggg acctcggccc ggtggacatc ctcgtcaaca atgccggaat tacgcgcgat
     3721 atgaccttca ggaagatgac caagggcgac tgggacgcgg tcctgcacac gaatctcgat
     3781 tccgtcttca acatgaccaa gcaggtctgt gacgcaatgg tggaccgcgg ctggggccgc
     3841 gtcatcaacg tgtcctcggt gaacggctcg aagggcgctt tcggccagac caactacgct
     3901 gcggcaaagg ccggcatgca cggcttcacg aagtcgctcg cgctggaagt ggcaaagaag
     3961 ggggtcaccg tcaataccat ctcgccgggc tacatcggga cgaagatggt taccgcgatt
     4021 ccgaaggaga tcctcgactc gaaaatcctg ccgcagattc cgctcggacg gctcggcaag
     4081 cccgaagaga tcgccgggct gatcatttac ctctgctcgg aggaggctgc gttcgtcaac
     4141 ggcgcaaaca tcgccatcaa cggcgggcag cacatggcgt aggcggactg gaggggccga
     4201 ctggatggaa cctagcggca atctccagta tcatgtggca ccgcaacacc cgtgcgacac
     4261 catgaggcaa gggtcccatg tctgagcccg cccgactgat caagaagtac cccaaccgtc
     4321 ggctctacga tacgaagacg agcgtttaca tcacgctcgc cgacgtgaag aagctcgtga
     4381 tgggaggcga ggaattccag gtggtggacg cgaagaccgg agacgacctg acgcgatcca
     4441 tcctcctgca gatcatactg gaggaggaag ccgctggagc gccgatgttt tcctcggacg
     4501 ttctcacgca gttcatccgc tcctacggga acgccatgca gggtatgctc ggggcgtatc
     4561 tcgagcgcaa catgcaactg ttcggcgaga tccagaagag catgcgggag cagtcacaga
     4621 agctctatgg agaacccacc aagggcaacg aagagctttg gaagcagttc atgagcttcc
     4681 agggcccggc gatgcagagc ctgatctcgg cgtacatgga gcagagccgc accgtgttcc
     4741 agcagatgca ggaacagctg cagaaccaga cgcgcaacat gttctccggc ttcgctttcc
     4801 cgggatttcc cggagggccg ggattcggcg cgcacccggc cggcgcgcag gcgtcgcagg
     4861 gcgacaacaa gacgcccggc gaggagaaaa agtcctgaaa tttcacaccc tggtgcaagg
     4921 tttaatctga agcatggccg cagcaccgaa ggtcgggttc gtctcgctcg gctgtcccaa
     4981 agctctggtc gattctgaag gcatcctcac gcgcctgcgc gcggaaggct acgcgatctc
     5041 gccttcgtac gatggcgcgg acctcgtcgt cgtcaacacc tgcgggttca tcgagtctgc
     5101 catgcaggaa tcgctggatg cgatcggcga agcgctcgag gaaaacggcc gcgtgatcgt
     5161 caccggatgt ctgggcgcca agggcgacgt cgtgcggcgg acgcacccga aggtccttgc
     5221 cgttagcggc ccacacgcgc tcgaggaagt catgacggcg gtgcacgagc atttgccgaa
     5281 accgcatgac ccatttgccg acctcgtgcc gccccagggc atcaagctca ctccgcggca
     5341 ctatgcctac ctgaagatct ccgaaggctg caatcaccgc tgcaccttct gcatcatccc
     5401 gtctctgcga ggcgatctgg cgagccggcc gatcggcgag gtgatgcgag aagcggaaaa
     5461 ccttgtgaag gcgggcgtga aggagctcct ggtgatttcc caggacacga gcgcctacgg
     5521 cgtcgatctc aagtaccgca ccggtttctg gggcgggcgg ccgctcaagg cgcgcatggg
     5581 cgatctggcg acggcattag gagaactggg cgtgtgggtg cggctccact acgtgtaccc
     5641 ctatccgcac gtggacgacg tgatcccgct catggcggat gggaagatcc tcccttatat
     5701 cgacgttccg tttcagcacg cgagccggcg gatcctgaaa ctcatgaagc gtcccgcgag
     5761 cggggaggac aatctcgggc gcatccgcgc ctggcgcagg atctgtccgg acatcacgat
     5821 ccgcagcacc ttcatcgcgg gattcccggg cgagaccgaa gacgaattcc gggaactact
     5881 cgactttctc gaggaagccg agctcgaccg cgtcggctgc tttgcgtatt cgccggtgga
     5941 aggcgcaaaa gcgaacgagc tgtctggcgc gctgccggac gaagtgcgcg aggaacgccg
     6001 gatgcggctc atgcgcctgc aggagaaaat cagtgcgaag cgtctgaaac gccacgtcgg
     6061 caggatcatc aaggtgctgg tggacgaggt gtcggccgga ggtgcggtgg cgcgctcaag
     6121 cgccgacgcg ccggagatcg acggcgtggt gcgcatggag gaggcctcga agctcaagcc
     6181 gggcgattgg gcgcaggtga agatcctgtc ttctgacgcg cacgacttgc gggcgcggct
     6241 ggttcccggg aaatcgaagc gaagtgttgc ggcgcaagct tcatgaaaca aggagatttc
     6301 catggcgcaa cgtgaagtgg tcgtgacgag cggcgttcgc accgcgatcg gcgactacgg
     6361 cggctcgctc aaggacactc cgccgacgaa gctcggcgcg atcgcgatca aggaagcggt
     6421 ggcgcgggcg aagatcgacc cggcggcggt cggtcacgtc gtactcggca gcgtgatcca
     6481 cggcgaggcg cgcgacatgt acatctcgcg cgtcgcggcg atcgaggccg gcctgccggt
     6541 cggcacgccc tgcctgacgg tcaaccgcct gtgcgggagc ggattgcagg cgatcgtctc
     6601 tgcctcccag catatcctgc tcggagacac cgacgtcgtg atcggcgcgg gtgccgagag
     6661 catgacccgt gcggcgtact tcatgccttc ggcgcgctgg ggccagcgca tgggtgacgc
     6721 gcaactcatc gatgccatga ccggagcact gcacgatccg ttcggcagcg gccacatggg
     6781 cgtgacagcg gaaaatatcg cggcgagata cggcgtgact cgcgatgaac aggatgcgtt
     6841 ctcgctcgag agccacaagc gcgcggcaag agcgacggat gccggctggt tcaagagcca
     6901 gatcgtgccg gtggaagtga aatcgaagaa aggacccgcg cagttcgaca ccgacgagca
     6961 tgtgcgcaag gacctgaaga tcgaggacct gcagaagctg aagacggtgt tcaagaagga
     7021 cggcaccgtt acagcgggca acgcctccgg catcaacgac gccgcggctg ccgtcgtgct
     7081 catggaaaaa tccgcggcgc aaaggcaagg cgccaagccg cttgcccgcc tggtcgccta
     7141 cgcgcacgcg ggtgtcgagc ctcagctgat gggcctcggg ccgatccccg cggtaaggcg
     7201 cgtcttcgag aaagcgggtc tcaagcccgc cgacatggac gtgatcgagt cgaacgaggc
     7261 cttcgcggtc caggcgatgg cggtgacgcg cgatctcggg ctcgatccgg cgaaggtcaa
     7321 tcccaacggc ggagcggtgg cgctcggcca cccgatcggc gcaaccggct gcatcctcac
     7381 cgtgaaggcg atttacgagc tgcaccgcac cggcaagcgc tacgccctgg tcacgatgtg
     7441 catcggcggt gggcagggga tcgcggcgat tttcgagcgg atgtgatgga gctcaggccc
     7501 cggcggcgac ccaagatcgg attcgtcctc ggcgcgggct cggcgcgcgg ctgggcgcat
     7561 atcggcgtgc tgcgcgccct caccgaggcg ggcatcaggc cggatgtcat cgcgggatgc
     7621 tcggtcggcg cgttcgtcgg agccgcgttt tctgcgggcc ggctcgacca gctggaggcg
     7681 tgggcgctcg cgctcgactg gaagcgcatg ctcaagctcg cggacttcgg cctgcgcggc
     7741 gggctcatca agggcgagcg cgtgaacgag gtctttcgcg agcagttcgt cgagtgcgag
     7801 ttctctgagc tgcctatacc atttgcggcg gttgcgaccg atctgcattc cggacaggag
     7861 atgtggctgc gcgagggcaa ggtgtcggtc gcgatcggcg cttccatagc cgtgccgggc
     7921 ctgtttcgtc cggttccgta cgacgatcgc tacctggtcg acggaagcgt cgtcaatccg
     7981 atcccggtct ccctgtgcag ggcgatgggg gcggacatcg tcctcgcggt cgatctcggg
     8041 tccgacctag tcggccgcta cgtgggcgac gatgacaggg cgccgacacg taccggcccc
     8101 cgcggattca tcagcagggt cctgccgcga ttcaaccgga caggagagag gcaagccgag
     8161 ccgccggccc tgctcgagac gctcatgggt tcgatcaaca tcatgcagct gcggatcgcc
     8221 cgcagcaggc tcgcgggcga gcctcccgac gttctgctca cgccgcgcct cggcaatctc
     8281 ggcctgctcg attaccaccg ggcgcaaatc gcgatcacgg aaggccgcga ggcggtcgtg
     8341 cgcatgctgc ccgcgatccg cagcgcgatt cccgcctgag tcgcggtacc ggtcctagcc
     8401 ggattgaagc tgacgaacga aaccctc
//
LOCUS       DASPZD010000475         8889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1677536, whole genome shotgun sequence.
ACCESSION   DASPZD010000475 DASPZD010000000
VERSION     DASPZD010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677536"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /gene="infB"
                     /locus_tag="VEM33_09195"
     CDS             <1..1539
                     /gene="infB"
                     /locus_tag="VEM33_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYS52045.1"
                     /translation="DVPEHHEAALMPRAPVVTVMGHVDHGKTSLLDYIRRTKVASGEA
                     GGITQHIGAYHVQTPRGMVTFLDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQT
                     IEAIHHARAAEVPIVVAINKIDKHEANQEKVKQELVAQAVVPEEYGGDSPFVPVSAKT
                     GAGIDTLLEQVLLQAEVLELKAPVDTLAKGLVIEARLDKGRGPVATVLVQSGTLRRGD
                     IVLAGSSFGRVRAMLDESGSAVHEAGPSIPVEIQGLSDVPAAGEDVMALGDERKAREI
                     ALFRQGKFRDVKLAKKQAAKLENLFEHMGEGEARVLALLIKADVQGSQEALVHALSRL
                     STDEVKVNVVHSAVGAITESDVNLALASKAVIIGFNTRADTAARKAAEASGVDVRYYN
                     IIYDAVDEVKAALSGMLAPEKKENILGLVDVRQVFKISKVGAVAGCMVLEGLVRRNAS
                     VRVLRDNVVVHTGEIDSLKRFKDDVREVKAGFECGMSVKNFNDLKVGDQFEIYEVQEV
                     ARTL"
     gene            1617..1976
                     /gene="rbfA"
                     /locus_tag="VEM33_09200"
     CDS             1617..1976
                     /gene="rbfA"
                     /locus_tag="VEM33_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYS52046.1"
                     /translation="MANPQRLRRVADQIQRELSDLLRAELKDPRVGMITLTGVEVSPD
                     LAYAKVFFTALADAESLARIEKGLEHAAGFLRVQLGRRLRLRVTPEIRFMHDASVERG
                     VRLSQLIDAAVTGKKAD"
     gene            1983..2873
                     /gene="truB"
                     /locus_tag="VEM33_09205"
     CDS             1983..2873
                     /gene="truB"
                     /locus_tag="VEM33_09205"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYS52047.1"
                     /translation="MGNTHAAINGVLLIDKPAGPSSNAVLQRAKRLLGAGRAGHTGTL
                     DPLASGLLVIVLGEATKFSGGMLDADKSYRAQLKLGERTETGDAEGAVLSRAEVKVTR
                     NELLAAVDRYRGEIEQLPPPYSAIKYRGRPLYAYARRGEHVPRAARRVAIRRLELERF
                     EGNTVGLFVECSKGTYIRVLAEDIGAELGCGAHLAALQRTSVGPFALRQAMTLETLEV
                     ISPAERRERLLPLEALLQSWPRLTLQAPLAAKFRQGRTMPVDSASGSVAVFGEDAKFL
                     GTGQVDASGTLQPIRLLALS"
     gene            2962..3231
                     /gene="rpsO"
                     /locus_tag="VEM33_09210"
     CDS             2962..3231
                     /gene="rpsO"
                     /locus_tag="VEM33_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HYS52048.1"
                     /translation="MRVSVTEKAQIVRQHQRAAGDTGSPEVQVALLTARINGLTDHFK
                     THVKDHHSRRGLLKMVSQRRKILDYLKRKNNGKYHELIEQLGLRK"
     gene            3360..5468
                     /gene="pnp"
                     /locus_tag="VEM33_09215"
     CDS             3360..5468
                     /gene="pnp"
                     /locus_tag="VEM33_09215"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HYS52049.1"
                     /translation="MRLTPIRKTFTYGAHQVTLESGEMARQAHGAVVVNMDDTVVLCS
                     VVGAKQAKAGQDFLPLTCDYVEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRP
                     LFPDGFYNEVQVVAQVLSCNPEVDPDIPALIGVSAALTISGIPFNGPIGAARVGRVND
                     EYVLNPTATQLKESRLNLVVAGTEHAVLMVESEASELPEDVMLGSVVFGHQQMQAAIK
                     AINDLADEAAKPAWDWKPAAKDEALAERIRELSESDLRAAFKLKQKQARSEAIDAIWK
                     RVFEAVGVGAEGGPEANAVKDICFALESKIVRNQILDGEARIDGRDTRTVRPINARVG
                     VLPRTHGSALFTRGETQALVVATLGTGRDEQIIDALMGEYKERFMLHYNMPPYATGET
                     GRMGSPKRREIGHGRLAKRALAAMLPTAEEFAYSMRVVSEITESNGSSSMASVCGGSL
                     ALMDAGVPMKAHVAGIAMGLIKEGKRFSVLTDILGDEDHLGDMDFKVAGTENGITALQ
                     MDIKIEGITKEIMQVALAQAREGRMHILGIMKEALEGSRAEISSYAPRIIKIKINPEK
                     IRDVIGKGGVVIRGLQEETGTTIEISDDGTVSIACVSAEGGQAAKKRIEDITADVEVG
                     RVYDGTVLKLLDFGAIVQLLPGKDGLLHISQIANERVNNVSDYLKEGQQVKVKVLEAD
                     EKGRVRLSMKAVKETAAAQS"
     gene            complement(5555..5974)
                     /gene="dksA"
                     /locus_tag="VEM33_09220"
     CDS             complement(5555..5974)
                     /gene="dksA"
                     /locus_tag="VEM33_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HYS52050.1"
                     /translation="MELLHKSFPPYVLKKGEEYMNPRQHAHFRKVLEALKVELSQDID
                     RTVHEMQDDATVFADPNDRASQESDIALELRNRDRERKLIRKIDETIARIDKDDYGYC
                     ENCGIEIGLKRLEVRPTATLCIDCKTLDELREKQVAR"
     gene            6118..6194
                     /locus_tag="VEM33_09225"
     tRNA            6118..6194
                     /locus_tag="VEM33_09225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6152..6154,aa:Pro,seq:tgg)
     gene            6241..6318
                     /locus_tag="VEM33_09230"
     tRNA            6241..6318
                     /locus_tag="VEM33_09230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6276..6278,aa:Arg,seq:tct)
     gene            6360..7064
                     /locus_tag="VEM33_09235"
     CDS             6360..7064
                     /locus_tag="VEM33_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HYS52051.1"
                     /translation="MIEVFTWPTPNGHKIHTMLEETGLEYEVRPVNIRVGEQFRPEFL
                     RISPNNKIPAIVDRNGPDGKPISVFESGAILIYLAGKTGKFLPRDDRGKYVVLQWLML
                     QMGSVGPMMGQAGHFRSNAVPERIEYAINRYTNEVKRLHRVMEKRLSEAAYFGGSEYS
                     IADIAIFPWLRASERNGIDWTEFPRVKKWFDGIAARPAVQRALKVLADVQTTPPGQYD
                     PKAREILFGATQYQRH"
     gene            7317..>8889
                     /locus_tag="VEM33_09240"
     CDS             7317..>8889
                     /locus_tag="VEM33_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYS52052.1"
                     /translation="MGQELRVLMVEDVESDAELAERELKRAGLAVRARRVEREEDFRR
                     EVGEFRPHVILSDFSMPQFNGMSALRIAREIVPDVPFIFVSGTLGEDYAIRALKNGAT
                     DYVLKTNLVRLPPTIERAVSEAADRAERRREEQLLALEHTVVRHIAGADSASVGLKAI
                     IRAICGSERWDVGRYFRADEKGGALRFSEAWSVAGSDIERFIEKSHDICYAPGVGPAG
                     QVWQSGQPLWVADITRDSSIEEAGLARSAGLHGGFVLPVNAEGKTIGVLAFNSREVRE
                     PEDRLLRAMHVIGSQIGQFLARQEQQRHIARLNRIYAVLSGINSTIVRVREREELLRE
                     ACRIVVRAGGFRLAWLGLVDAKANRVKPMAWDGVGEDYIGMIPLGLDEKAPGDYGLAG
                     RAVKERKAMIVDDMTADPRITLRKETLERGFRSLAILPLLVAEEPAGVLALYADTPSF
                     FDSEDEMRLLRELAGDIAFALEHIEKAEQLNYLAYYDPLTGLANATLFRERLARFVDA
                     ARGAGHKLALGLVDVD"
BASE COUNT         1739 a         2718 c         2925 g         1507 t
ORIGIN      
        1 gacgtgcccg agcaccacga agccgcgttg atgccccgag ctcccgtggt gacggtcatg
       61 ggacatgtgg accacggaaa gacatcgctg ctcgattaca tccgccgcac caaggtcgcc
      121 tcgggtgaag cgggtggcat cacccagcac atcggcgcct accacgtcca gacgccgcgc
      181 ggcatggtca cattcctcga tacgccgggg cacgaggcgt ttacggcgat gcgcgctcgc
      241 ggtgcaaaag tgaccgacat cgtcgtgctg gtggttgcag cggacgacgg cgtgatgccg
      301 cagacgatcg aggcgatcca tcacgcgagg gcggccgaag ttcccatcgt cgtggcgatc
      361 aacaagatcg acaagcatga ggcgaaccag gagaaggtca agcaggagtt ggtagcgcag
      421 gccgtggtgc ccgaagaata cggcggggat tcgccgttcg tccccgtgtc ggcgaaaacc
      481 ggcgccggaa tcgacacgct gctggaacaa gtgttgctgc aggccgaggt actggaattg
      541 aaggctccgg tggacacgct cgccaaaggc ctcgtgatcg aggcacggct ggacaagggg
      601 cgaggaccgg tagccacggt gctggttcaa tccggaaccc tccggcgggg cgatatcgtg
      661 ctcgccggga gcagcttcgg ccgcgttcgc gccatgctag atgagagcgg aagcgcggtc
      721 cacgaggccg gtccatcgat tccggtcgag atccagggac tgtcggacgt gcccgcggca
      781 ggcgaggatg tcatggcgct cggcgacgag cgcaaggcgc gcgagatcgc gctgttccgt
      841 caaggcaagt tccgcgacgt gaagctcgcg aagaaacagg ccgccaagct ggaaaacctg
      901 ttcgagcaca tgggcgaagg cgaggcgagg gtgctcgcgc ttctcatcaa agccgacgtc
      961 cagggctcgc aggaggcgct cgtccatgcg ctcagccgcc tctcgaccga cgaggtcaag
     1021 gtcaacgtgg tgcacagcgc ggtgggcgcg atcaccgagt cggacgtgaa tttggcgctc
     1081 gcttccaagg ccgtcatcat cggcttcaat acccgtgccg atacggctgc gcgcaaggcg
     1141 gccgaggcct cgggggtgga cgtacgctac tacaacatca tctacgacgc cgtcgacgaa
     1201 gtgaaggcgg cgctgtcggg catgctcgcg ccggagaaaa aagaaaacat actcggactg
     1261 gtcgacgtgc gccaggtatt caagatctcc aaggtcgggg ccgtcgccgg atgcatggtg
     1321 ctcgagggcc tggtgcggcg caacgcatcg gtgcgcgtgc tgcgcgacaa cgtcgtggtc
     1381 catacgggcg agatcgattc gctcaagcgt ttcaaggacg acgtgcgcga agtgaaggca
     1441 gggttcgagt gcggcatgtc cgtgaaaaac ttcaacgacc tcaaggtcgg cgaccagttc
     1501 gaaatctacg aagtccagga agtggcgcgg acgctctgac gcccacgcct tcccgaggag
     1561 cgccgagcgc gacgaaggat ccgctgtagg atggccgatc gcctagctcc gaccccatgg
     1621 ccaacccgca acgcctgcgc cgcgtcgccg accagatcca gcgcgagctg tcggacttgc
     1681 tgcgcgccga gctcaaggat ccccgcgtcg gcatgattac actcaccggg gtcgaggtct
     1741 ccccggacct tgcctacgcg aaagtcttct tcacggcatt ggccgatgcg gagtccctgg
     1801 ccaggatcga aaaagggctc gagcacgcgg ccggcttcct gcgcgtccag ctcgggaggc
     1861 gcttgagact ccgcgtcact ccggaaatac gcttcatgca cgacgcttcg gtcgagcgag
     1921 gcgtgcgtct ctcgcagctg atcgacgcgg cagtcaccgg aaagaaggcg gattgagcct
     1981 ccgtgggaaa tacgcacgcg gcaatcaacg gggtgctgct catcgacaag ccggcaggac
     2041 cgagctcgaa tgcagtattg caaagagcga agcgcctgct cggggcgggg cgtgcgggcc
     2101 atacgggaac gctcgaccct ctcgcaagcg gtttgctcgt cattgtgctg ggcgaagcga
     2161 ccaagttttc cggcgggatg ctcgatgcgg acaagagcta tcgcgcgcag ttgaagctcg
     2221 gcgagcgcac cgagaccggt gacgccgaag gtgcggtgct ttcgcgcgcc gaagtcaaag
     2281 taacccgcaa cgagctgctc gccgcagtcg atcgctatcg aggcgagatc gagcagctgc
     2341 cgcctccata ttccgcgatc aagtatcgcg gccggccctt gtacgcctac gcccgccgcg
     2401 gcgagcacgt tccacgcgcc gctcggcgcg tcgcgatccg ccgactcgag ctggagaggt
     2461 tcgagggtaa tacggtcggc ctgttcgtcg aatgcagcaa gggcacctac attcgggtgc
     2521 ttgccgagga tatcggcgcg gaactcggct gcggcgcaca tctcgcggcg ctgcagcgca
     2581 cctccgtggg cccgttcgcg ttgcggcagg cgatgacgct ggaaacactc gaggtgattt
     2641 cgcctgcgga gcgacgcgag cggctcctgc ccctggaagc cttgctccaa tcctggccgc
     2701 gcctcacgct ccaagccccc cttgccgcca agttccggca ggggcggacg atgccggtcg
     2761 actctgcaag cggcagcgtc gccgttttcg gcgaggacgc gaagttcctc ggcactgggc
     2821 aagtggatgc aagcggcacc ttgcagccga tacggctgct cgcgctgtct tgacgtgttt
     2881 tccgattcaa aactgcttgt ggaaggcgct gatttcaagg taaaatgtcg gcctttcgcg
     2941 aatagataat agagccagag catgcgggtc agcgttacgg aaaaggcgca aattgtccgg
     3001 cagcaccagc gcgcggcggg cgatacgggt tccccggaag tgcaggtcgc cttgcttaca
     3061 gcccggataa acgggctgac cgaccacttc aagacgcacg tcaaggatca ccattcgcgg
     3121 cgcggattgc tgaagatggt gagccagcgt cgcaagatac tcgattacct caagcggaag
     3181 aacaacggga aatatcacga gctgatcgag caacttggcc ttcgcaagta aatccccgag
     3241 cccatgacca cgatgggcgt tttgatttcc agatacggtc cggcgcccct gcgtcggcac
     3301 cggcctcgtc aaaatggccg ggcacggcgc ccgagctcct tctacggatc gagaaaatca
     3361 tgcggctaac ccccatcagg aagacattca cctacggcgc gcaccaggtg acgctggaat
     3421 ccggcgaaat ggcacgccag gcgcacggag cggtcgtcgt gaacatggac gacaccgtcg
     3481 tgctgtgcag cgtggtcgga gcgaagcagg ccaaagcggg ccaggacttc cttccgctca
     3541 cctgcgacta cgtcgagaag acctacgccg cgggcaagat tcccgggggg tttttcaagc
     3601 gcgagggcag gccttcggag aaggagacgc tgacctcgcg gctgatcgac cggcccattc
     3661 gcccgttgtt tccggacggg ttctacaacg aagtgcaggt cgttgcgcag gtattgtcgt
     3721 gcaatccaga agtcgatccg gacattccgg cgttgatcgg ggtctcggct gcgctcacca
     3781 tttccgggat tcctttcaac ggtccgatcg gggccgcgcg cgtcggccgc gtcaacgacg
     3841 aatacgtgct caaccctacg gcgacgcagc tcaaggagag ccggctcaac ctcgtggtcg
     3901 cgggcaccga gcacgcggtc ctgatggtgg aatccgaggc gagcgagctg cccgaggacg
     3961 tgatgctggg gtcggttgtg ttcgggcacc agcagatgca ggccgcgatc aaggcgatca
     4021 acgacttggc ggacgaagcg gcgaagccgg cctgggattg gaaacccgcc gcgaaggacg
     4081 aagcgctcgc cgagcgcata cgcgagcttt cggagagcga cctgcgcgcg gcgttcaagc
     4141 tcaagcaaaa gcaggcccgc tccgaggcga tcgacgcgat atggaaacgc gtgttcgagg
     4201 ccgttggggt cggcgccgaa ggcggacccg aagcgaacgc cgtcaaggat atttgcttcg
     4261 cgctcgaatc gaagatcgtc cgcaatcaga ttctcgacgg agaggcgcgc atcgacggcc
     4321 gcgatacgcg cacggtgcgc ccgatcaacg ctcgcgtggg cgttctgccc cgcacgcacg
     4381 gctcggcgct cttcacccgc ggcgagaccc aggcgctggt cgtagccacg ctgggcaccg
     4441 gccgcgacga gcagatcatc gacgcgctga tgggcgagta caaggagcgc ttcatgctcc
     4501 actacaacat gccgccctat gccaccggcg agaccggccg catgggttcg cccaagcgcc
     4561 gcgagatcgg acacggacgg ctcgcgaagc gcgctctcgc cgccatgctc cccacggccg
     4621 aggagttcgc gtactccatg cgcgtggtct cggagatcac cgagtccaac ggatcctcct
     4681 cgatggcctc ggtctgcggc ggcagcctcg cgctcatgga cgccggcgtt ccgatgaagg
     4741 cccacgtcgc gggcatcgcg atggggctca tcaaggaagg aaagcgcttc tcggtcctca
     4801 ccgatatcct gggcgacgag gaccacctcg gcgacatgga cttcaaggtc gcgggcaccg
     4861 agaacggcat caccgcgctg cagatggaca tcaagatcga aggcatcacc aaggagatca
     4921 tgcaggtggc gctcgcacag gcgcgcgagg gccggatgca catcctcggc atcatgaagg
     4981 aggcgctgga gggatcgcgg gccgaaatct cgagctacgc accgcgcatc atcaagatca
     5041 agatcaatcc cgagaagatc cgcgacgtca tcggcaaggg cggtgtggtc atacgcgggc
     5101 tgcaggagga gaccgggacc acgatcgaga tctccgacga cgggaccgtc tcgatcgcct
     5161 gcgtctccgc cgagggcggc caggcggcga agaagcggat cgaggacatc acggcggacg
     5221 tcgaggtggg ccgggtgtac gacgggaccg tgctgaaact gctcgacttc ggagccatcg
     5281 tgcagctgct tcccggaaag gacggcctgc tgcacatttc gcagatcgca aacgagcgcg
     5341 tcaacaacgt atccgactat ctcaaggaag gccagcaggt gaaagtcaag gtgctcgagg
     5401 ccgacgagaa ggggcgggtg cgcctcagca tgaaggcagt gaaggagacg gccgcggcgc
     5461 aatcctgatt cgagggtcaa aaatggaaat ggcgccgtcg gcgccatttt tttcgtaact
     5521 cccacgcgag cgggagtcca ggatggtccg ccagttacct tgccacctgc ttttccctca
     5581 gttcgtcgag cgtcttacag tcgatgcaca aagtcgcggt gggccgcacc tccagtctct
     5641 taaggccgat ctcgatgccg cagttttcgc agtagccgta gtcgtccttg tcgatgcgcg
     5701 cgatcgtttc gtcgatcttc ctgataagct tgcgctcgcg gtcacggttg cgcagctcga
     5761 gggcgatgtc ggattcctgg ctggcgcgat cgttcggatc ggcaaacacg gttgcgtcgt
     5821 cctgcatctc gtggacggtt cgatcgatat cctgcgacaa ctccactttc agcgcttcga
     5881 ggactttcct gaaatgcgcg tgctgtctcg gattcatgta ctcctcaccc tttttcagga
     5941 cgtagggcgg gaaggatttg tggagcagct ccaattcgca atctccgcgc gaggcaatga
     6001 ggcgaatgca atttgatacc agaaatcaac agagggggca agccactttt cacctggaag
     6061 ttgccggttc ttcagcgttg accgtgccag ggaccaaggc gtaagatgcg acacccgcgg
     6121 ggtgtagcgc agcctggtag cgcgcttgct ttgggagcaa gatgtcggga gttcaaatct
     6181 ctccaccccg accagctttt ttttgaccgc cgccgaggcg gccgcagtag gtttttgccc
     6241 gtgcccgtag ctcaaactgg atagagcacc agccttctaa gctgggggtt gtgggttcga
     6301 ctcccgccgg gcgcgccagt cttttccgag acatccctgc aaacgaaagg aaatccccga
     6361 tgatcgaagt ctttacttgg cccacaccca atgggcacaa gatccacacc atgctcgagg
     6421 aaaccgggct cgagtacgag gtccggccgg taaacatccg cgtcggcgag cagttcagac
     6481 cggagttcct gagaatcagt ccgaacaaca agatccctgc gatcgtggac cgaaacgggc
     6541 cggacggaaa gccgatctcg gtcttcgagt cgggtgcgat cctgatttac ctggccggga
     6601 agacgggcaa gttcctgcct cgcgacgacc gcggcaagta cgtggtcctg caatggctga
     6661 tgttacagat gggatcggtc ggcccgatga tggggcaggc cggacatttc cgaagcaacg
     6721 ccgtccccga gcgcattgag tacgcgatca accgctacac gaacgaggta aagcggctgc
     6781 accgcgtgat ggaaaagcgg ctctccgagg cggcgtactt cggagggtcg gagtattcga
     6841 tcgcggacat cgcgattttt ccgtggctgc gcgcaagcga gcgcaacgga atcgactgga
     6901 cggaatttcc cagggtgaag aagtggttcg acgggatcgc ggcgcggccc gcggtgcaac
     6961 gcgcgctgaa ggtgctcgcc gatgtgcaga cgaccccgcc cggccagtac gatcccaaag
     7021 cgcgcgagat cctgttcggc gcgacgcaat accagcgtca ctgagaggta tccgacggcg
     7081 caggtttgct ttatcgctga cgtggccctt tcgatctcgt cgacgcgttt gtagagtttt
     7141 cgctacgttt tccatcgggt tttcctgaca gcaatcccgg atttcgaaga aaaagcacga
     7201 atttccgata gataacctcg cggaaagctt gctgttttta cactgttccc ctatggtcgc
     7261 ttggggaagc gaagactcga agaagtcggg aagttcagca agctcaagga gggcctatgg
     7321 gccaggaact ccgagtcctg atggtggaag acgtcgagag cgacgccgag ctcgcagaac
     7381 gcgagctcaa gcgcgcaggc ttggccgtgc gcgcgcgccg cgtggagcgc gaggaagact
     7441 tccggcgcga ggtcggcgaa ttccgtcctc atgtcatcct ctccgacttc agcatgccgc
     7501 aattcaacgg catgagcgcg ctcaggattg cgcgcgagat cgttcccgac gttcctttca
     7561 tcttcgtctc cggaaccctt ggtgaggact acgccatccg ggcgctgaag aacggcgcga
     7621 ccgactacgt cctcaagacc aacctcgtgc gtctcccgcc cacgatcgag cgggcggtga
     7681 gcgaggctgc cgaccgcgcg gagcgcaggc gcgaggagca attgcttgcg ctcgagcaca
     7741 ccgtggtccg gcacatcgcg ggcgccgaca gcgcgtcggt cggcctgaag gcgatcatcc
     7801 gcgcgatatg cgggtctgag cgctgggacg tcggacggta tttccgcgcg gacgagaagg
     7861 gcggggcgct gcggttcagc gaggcctgga gcgtcgccgg ctccgacatc gaacggttca
     7921 tcgaaaaatc gcacgacatc tgttacgccc ctggcgtcgg cccggcaggc caggtgtggc
     7981 agtcgggaca gcctctctgg gtcgccgaca tcacccgcga ttccagcatc gaggaggccg
     8041 ggcttgctcg cagcgcgggt ttgcacggcg ggtttgttct tcccgtgaac gccgaaggca
     8101 agacgatcgg cgtgctcgcc ttcaacagcc gcgaagtccg ggagccggag gatcggctgc
     8161 tgcgtgccat gcacgtgatc ggcagccaaa tcgggcagtt tctcgcccgc caggagcagc
     8221 aaaggcacat cgcccgcctc aaccggattt acgcggtgct gagcggaatc aactcgacca
     8281 tcgtgcgcgt gcgcgagcgc gaggagctgc ttcgcgaagc ctgccggatc gtggttcggg
     8341 ccgggggatt ccggctggca tggctcggcc tcgtggacgc gaaagcgaat cgggtgaaac
     8401 ccatggcctg ggacggagtc ggcgaggact acatcggaat gataccgctc gggctcgacg
     8461 agaaagcgcc cggggactac ggccttgccg ggcgcgcggt caaggagcgc aaggcaatga
     8521 tcgtcgacga tatgaccgcg gacccgcgga tcacgctccg aaaagaaacg ctcgagcggg
     8581 gctttcgctc gctcgccatc ctgcccttgc tggtagcgga ggagccggcc ggggtgctcg
     8641 ccctgtacgc cgacacgccg agttttttcg actccgaaga cgaaatgcgc ctgctgcgcg
     8701 agcttgccgg cgacatcgct ttcgcgctcg agcacatcga gaaggccgag cagctcaact
     8761 accttgccta ctacgatccg ctcaccggcc tcgcgaacgc gacgctcttc cgcgagcgcc
     8821 tcgcgcgctt cgtggacgcg gcgcgcgggg cagggcacaa gctcgccctc gggctcgtgg
     8881 acgtggacc
//
LOCUS       DASPZD010000476         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_987618, whole genome shotgun sequence.
ACCESSION   DASPZD010000476 DASPZD010000000
VERSION     DASPZD010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987618"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VEM33_09245"
     CDS             <1..451
                     /locus_tag="VEM33_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS52053.1"
                     /translation="CSTVVSEFRIAQGKQHIITLEDRFDRPEVTMDERLLRHILNNLL
                     SNAVKYSPPGSEVTFSLSRHDEKAAIEIQDRGIGIPLEDQPRMFESFHRASNVENRPG
                     TGLGLAIVKKAVELHGGEIGLTSAVGSGTRFTVMIPLRPVEQPAGVS"
     gene            451..2517
                     /locus_tag="VEM33_09250"
     CDS             451..2517
                     /locus_tag="VEM33_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYS52054.1"
                     /translation="MADESRKKVLVIEDEPSIRNNIMLMLKVERYAAAGAENGRVGLE
                     LARRDPPDLILCDVMMPEMDGFAVLEALRAEPRLAEIPFIFLTALDDRSSTRRGMNLG
                     ADDYLPKPFTRNELMEAVNSRLKKFENLTQALAARLVLKQDRLTQKFREKISGGDGTA
                     ALEETDPGIPTGKIAEASVLFSDIRNFTTYSERLTSDEIAELLNAYLQTACAPIIRCR
                     GVVMKFIGDGVMALFESSHGEGGESHAQRAVRAGLAMQLAALEFREWVKRHHPSQSLP
                     EFSIGVGIHSGEVLLCHVGTPGRGELTVIGDTVNIASRLEGQTKELGWAVVASEATVN
                     AAGSALVVGSRRTVQLRGRSAPTDAVEIIGIAAGDADHASVQIPEDMRQALAANARVA
                     ADAAKAALGITLSIMTGEIGEKRLTVQGYRVVSKVGRGGSSVVYLAERESDKRQVVLK
                     ILNATGGLDEILLQRFVQEFDIISSIDHPNVVKIYDRGFSDRHAYIAMEYFPNGSLAQ
                     IIPNGLSGRQALSLLAQAAGALREVHGRGIIHRDIKPGNLMARADGSIVLADFGIAKR
                     LGEDKGRTRQGELYGTPYYVSPEQIEGSPATMQSDIYALGIIFHEMLTGRRPFDAESV
                     SGLIALHVSAPRPRLPGELAEYQGLLDRMIAVDPRNRFKRADEVLEAIDEVWTQQALR
                     TMKQSS"
     gene            complement(2526..3290)
                     /gene="dapB"
                     /locus_tag="VEM33_09255"
     CDS             complement(2526..3290)
                     /gene="dapB"
                     /locus_tag="VEM33_09255"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HYS52055.1"
                     /translation="MGRTLIEMALASGELKLAAALEVPGSPVIGKDAGELIGRPCGVK
                     IASSFADAVAKGDCLIDFTRPEGTLTHLEQCLKKGVRMVIGTTGFSAEQDERIAAAAG
                     KIAIVKSPSMSAGVNVAFRLVETAARSLGEAYDVEILEAHHRHKVDAPSGTALKFGEA
                     ISRALGRDLARTAVYGRKGEVGKRDPKAIGFHSVRGGDIAGEHTVMFIGEGERMEITV
                     RSGSRATYALGALRAARFLENKESGLYDMQDVLGFR"
     gene            complement(3320..3670)
                     /gene="bamE"
                     /locus_tag="VEM33_09260"
     CDS             complement(3320..3670)
                     /gene="bamE"
                     /locus_tag="VEM33_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HYS52056.1"
                     /translation="MQRILILALPLLTAGCGLAPSLTPYRMEIQQGNYVTQEMVAQLK
                     PGLTRDQVRFVLGTPLVSDIFHEERWDYVFLRQRASSREIEYRRIAVFFEDGKMKRVE
                     GDTVAAASPGKREQ"
     gene            3745..>3872
                     /locus_tag="VEM33_09265"
     CDS             3745..>3872
                     /locus_tag="VEM33_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HYS52057.1"
                     /translation="MTNVHDLKTSGLKATGPRLRILKLFENSKVRHLSAEDVYRIL"
BASE COUNT          738 a         1268 c         1198 g          668 t
ORIGIN      
        1 ctgcagcacg gtcgtgtcgg aattccgtat cgcgcaaggc aagcagcaca tcatcaccct
       61 ggaggaccgc ttcgaccgcc cggaagtgac catggacgag aggctgctgc ggcatatcct
      121 gaacaacctc ctctcgaacg cggtgaaata ctctcccccg ggcagcgaag tgactttttc
      181 cctcagccgg cacgacgaga aggccgcgat cgagatccag gatcggggaa tcggaatacc
      241 cctggaagac cagccgcgga tgttcgagag ctttcaccgc gcctcgaacg tcgagaaccg
      301 cccgggcacg ggcctcggtc ttgcgatcgt gaagaaggcg gtggagcttc acggcggcga
      361 aatcggtctc accagcgcgg tcggctccgg aacccggttc acggttatga tccccctgcg
      421 gccggtcgag cagccggccg gagtaagctg atggccgacg aatccaggaa gaaagtcctc
      481 gtgatcgagg acgagcccag catccgcaac aacatcatgc tgatgctgaa ggtcgaacgt
      541 tacgccgcgg ccggagcgga aaacggccgc gtcggcctgg agctggcgcg cagggatccg
      601 cccgatctca tcctctgcga cgtaatgatg ccggaaatgg atggattcgc cgtgctcgag
      661 gccttgcgag ccgagccgcg cctggccgag attccgttta tcttccttac cgcactcgat
      721 gatcgatcga gtacccggcg cggcatgaac ctcggcgcgg acgactacct cccgaagccg
      781 ttcacccgaa acgagctcat ggaagcggtg aacagcaggc tgaagaaatt cgaaaacctg
      841 acccaggcgc tggccgcgcg gctggtcctg aagcaggacc gcctcacgca gaaattccgg
      901 gaaaaaatct ccggcggcga cggcaccgcg gccctcgaag agaccgatcc cggcatcccg
      961 accggcaaga tcgccgaagc ctcggtgctt ttctcggaca tacgcaactt cacgacgtat
     1021 tcggagcggc tcaccagcga cgagatcgcc gagttgctca atgcctacct ccagaccgcg
     1081 tgcgcgccga tcatccgctg ccgcggcgtc gtcatgaaat tcatcggcga cggggtcatg
     1141 gcgctgttcg agtcctctca cggcgaaggg ggcgaatccc acgcgcagcg ggcggtgcgc
     1201 gccggcctcg caatgcagct cgccgcgctc gaattccgcg aatgggtcaa gcggcaccac
     1261 ccttcgcaaa gcctgcccga attttccata ggcgtgggca tacacagcgg tgaagtgctg
     1321 ctgtgccacg tcggcacgcc gggccgcggg gagcttacgg tgatcggcga caccgtgaac
     1381 atcgcatcgc gccttgaagg tcagaccaag gagctcggat gggcggtagt cgcgagcgaa
     1441 gcgaccgtca acgcggccgg ctcggcgctt gtcgtgggct cgcgccgcac agtgcagttg
     1501 cgcgggcgca gcgcgcccac cgatgcggtc gagatcatcg gcattgcggc cggcgatgcc
     1561 gaccacgcct cggtgcagat cccggaggac atgcgccagg cgctcgcggc caacgcccgg
     1621 gtcgctgccg atgcggcaaa ggccgccttg ggcatcactt tgagcatcat gaccggagaa
     1681 atcggcgaaa agcgcctgac ggtccagggt taccgcgtcg tgtcgaaggt cggccgcggc
     1741 ggctcttcgg tcgtctacct cgccgagcgg gaaagcgaca agcggcaggt cgtcctcaag
     1801 atcctcaacg ccactggcgg tctcgacgaa atcctgctgc agcgcttcgt gcaggagttc
     1861 gacatcatct cgtcgatcga tcaccccaac gtggtcaaga tctacgatcg gggcttcagc
     1921 gatcgccacg cgtacatcgc gatggagtat tttcccaacg gcagcctcgc ccaaatcatc
     1981 cccaacggtc tgagcgggcg ccaggccttg tccctgcttg cccaggccgc aggcgcgctg
     2041 cgcgaggttc acggccgcgg catcatccac cgcgatatca agccgggcaa tctcatggcg
     2101 cgcgcggacg gcagcatcgt cctcgccgac ttcggcatag ccaagcggct cggagaggac
     2161 aaggggcgca cccgccaagg ggagctctac ggaaccccct attacgtttc accggagcag
     2221 atcgaaggca gccccgcgac gatgcagtcc gacatctacg ccctcggcat cattttccac
     2281 gagatgttaa cgggccggcg gcccttcgat gccgaatcgg tgtcgggatt gatcgcactg
     2341 cacgtcagcg caccgcgccc gaggctcccc ggtgagctgg ccgagtatca gggcctgctc
     2401 gacaggatga tcgccgtgga tccccgcaac cgcttcaaaa gggcggacga ggtgctcgag
     2461 gcgatcgacg aggtatggac gcagcaggcc ctgcggacga tgaagcaaag tagttgaggt
     2521 cctgcttagc gaaacccgag tacatcctgc atgtcgtaga ggccgctttc cttgttctcg
     2581 aggaagcgcg ctgctcgcag cgccccgagg gcataggtgg cgcggctccc cgagcgcacg
     2641 gtgatctcca ttcgttcgcc ctctccgatg aacatcaccg tatgctcacc ggcgatgtcg
     2701 ccgccgcgaa cggagtggaa cccgatcgcc ttgggatcgc gtttaccgac ctctcctttg
     2761 cgtccgtaca cggctgtgcg ggcgaggtcc ctgccgaggg cgcgggagat cgcttcgccg
     2821 aacttgagcg ccgtgcctga aggcgcatcg accttgtggc gatggtgtgc ttcgaggatc
     2881 tccacgtcat aggcctcacc gagcgagcgc gcggccgtct cgacaagcct gaacgcgacg
     2941 tttactcccg ccgacatgct cggcgatttc acgatcgcga tctttcccgc cgccgccgcg
     3001 atgcgttcat cctgttcggc cgaaaaaccg gtcgttccga tcaccatgcg cacgcccttc
     3061 ttcaggcact gttccaggtg cgtgagagtc ccttcaggac gcgtaaaatc gatgaggcag
     3121 tcgcctttcg caactgcgtc ggcgaagctc gatgcgatct tcacgccgca gggacgtccg
     3181 atcagttctc cggcatcctt gccgatgacg gggcttccgg gaacctccag cgctgccgcg
     3241 agcttgagtt cccccgatgc gagggccatc tcgatcagcg tgcgtcccat gcgccccccc
     3301 gcgccggcga tgacaagctt cactgttcgc gctttcccgg ggatgcggcg gccacggtat
     3361 ctccctccac gcgcttcatc ttcccgtctt cgaaaaacac cgcaatgcgg cggtactcga
     3421 tctcgcggct gctggcacgc tgacgcagaa agacgtaatc ccagcgttcc tcgtgaaaga
     3481 tgtcgctgac caggggcgtg cccagcacaa atcgcacttg atcgcgggtc agtccgggct
     3541 tgagctgcgc gaccatttcc tgggtgacgt aattcccctg ctggatttcc atccggtaag
     3601 gtgtgaggct cggcgcaagc ccgcaccccg cagtgagaag cggcaacgcc aaaatcagga
     3661 ttcgctgcac gatcagggtg tttccgcggc gatgaaacgc tatatgatag cggaaacggg
     3721 catctttccc cgaggtgatc ctccgtgacc aacgtgcacg acttgaaaac cagcggcctc
     3781 aaggccacgg ggccgcggct gcgaatcctg aagcttttcg aaaacagcaa ggtgcgccac
     3841 ctgtcggccg aagacgtcta tcggattctg at
//
LOCUS       DASPZD010000477         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_692179, whole genome shotgun sequence.
ACCESSION   DASPZD010000477 DASPZD010000000
VERSION     DASPZD010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692179"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VEM33_09270"
     CDS             <1..251
                     /locus_tag="VEM33_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYS52058.1"
                     /translation="VAVRAEGGAVVLDVADRGPGIPESEAERLKQPFTRLEVARSDKG
                     GAGLGLAIVERVVRAHRGTLELLAREGGGLVARIRLPG"
     gene            326..1267
                     /locus_tag="VEM33_09275"
     CDS             326..1267
                     /locus_tag="VEM33_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS52059.1"
                     /translation="MKRLLAVLAFALAPAAFAQTCPEKNLMYWQAFPAGGESDISARH
                     QQFVLKKKCPATETIIQYKPGVGGAVMWNQMNALPGDGLNIVGINLPHIVLAPFEGQV
                     QYKTEDITPTYWFHYTPDALVVPGSSPIKTFQDFLRTAKEEPGKLTLGGSGLNSANHA
                     AHERLNSEFGVKTLYVPFKGTGDMTIAVAGGHVSGAMSYTAFAINNKGKTRALAVAMD
                     KRHPLLPDTPTFKELGVNWVDGAFRGIGMPKSTPPAVRKRMSDLWAALNNDPEMKELA
                     AKSGFELVNVGVEQMDAFMKERIRVYTGVAKQMGLAK"
     gene            complement(1306..1650)
                     /locus_tag="VEM33_09280"
     CDS             complement(1306..1650)
                     /locus_tag="VEM33_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52060.1"
                     /translation="MKPNVAAPVDYSKRRHQPEKDDLELSPAGEALLAYIEESARPKE
                     LAAAFPRILNHMAKLWKMPREMDRYFGELLTDTRGKRKGFSLNILMELSTLNDYYKAK
                     TRTGKNDVWDYS"
     gene            complement(1739..>2091)
                     /locus_tag="VEM33_09285"
     CDS             complement(1739..>2091)
                     /locus_tag="VEM33_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYS52061.1"
                     /translation="AEVGRAAGEVAAPLASLVLDRPGYWKHNRIAWAGASLVPPELAA
                     LVSGLREALAKSRIGFDAKPFVSHVTLLRDAREPKGMPALDPIPWKIDGFALVESVTL
                     ARGSRYEVRRSWSA"
BASE COUNT          373 a          698 c          694 g          326 t
ORIGIN      
        1 gcgtcgcggt gcgcgccgag ggcggcgcgg tggtactcga cgtcgcggac cgcggcccgg
       61 gaattcccga atcggaggcg gagcggctga agcagccgtt cacgcgcctc gaggtcgcgc
      121 gcagcgataa gggcggcgcg ggcctgggcc tcgccatcgt cgagcgcgtg gtgcgcgcgc
      181 accgcggcac gctcgagctg ctcgcgcgtg agggcggcgg cctcgtcgcg cgcatccgcc
      241 ttccgggcta gagcaagggc ggccgttccg ggcggcggag gttctcgggt acacttcgaa
      301 gacaactcga aaggacaact ccgccatgaa gagactgctc gccgtactcg ctttcgcctt
      361 ggcccccgcg gcgttcgcgc agacctgccc ggagaagaac ctgatgtact ggcaggcgtt
      421 tccggccgga ggcgaatccg acatctcggc gaggcaccag cagttcgtgc tgaagaagaa
      481 atgccccgca accgaaacca tcatccagta caagccgggg gtgggcggcg ccgtcatgtg
      541 gaaccagatg aacgcgctgc cgggcgacgg cctgaacatc gtcggaatca acctgccgca
      601 catcgtgctc gcgcccttcg agggacaggt gcagtacaag accgaggaca tcacgcccac
      661 gtactggttc cactacacgc cggacgcgct ggtcgtgccc gggtcgagcc cgatcaagac
      721 tttccaggac ttcctcagga ccgcgaagga ggagcccgga aagctcacgc tcggcggctc
      781 cgggctcaac tcggcgaacc acgcggcgca cgagcgcctc aactccgagt tcggcgtcaa
      841 gaccctctac gtgcctttca agggcacggg cgacatgacc atcgcggtgg cgggcggcca
      901 cgtgagcggc gccatgtcct acaccgcctt cgcgatcaac aacaagggca agacgcgcgc
      961 gctcgcggtg gcgatggaca agcgccaccc gctgctgccc gacacgccca cgttcaagga
     1021 gctgggcgta aactgggtcg acggcgcctt ccgcggcatc ggcatgccga agtccacgcc
     1081 tcccgcggtg cgcaagcgca tgtccgacct gtgggcggcg ctgaacaacg acccggagat
     1141 gaaggagctc gccgcgaaga gcggcttcga gctggtgaat gtcggcgtgg agcagatgga
     1201 cgccttcatg aaggagcgca tccgcgtcta cacgggcgtt gcaaaacaga tggggcttgc
     1261 gaagtaaacc ggatcggcgg gcccgcgccc gccggggact tcaactcacg aataatccca
     1321 cacgtcgttc ttgccggtac gcgtcttggc cttgtagtag tcgttgagcg tgctcagctc
     1381 catgaggatg ttgagcgaaa aacctttgcg cttcccgcgg gtgtccgtga gcagctcccc
     1441 gaagtagcga tccatctccc gcggcatttt ccacagcttg gccatgtggt tgaggatcct
     1501 gggaaacgcc gcggccagct ccttgggccg cgccgattcc tcgatatacg cgagcagggc
     1561 ctcacctgcg gggctcaact cgagatcgtc cttttcaggc tggtggcggc gcttgctgta
     1621 atccaccggc gccgcgacgt tcggcttcat ggggaatgcg atgtccattc ggcgactcca
     1681 tccgtggtga actcataggc aaattacgcg gatttgcccc gtacgggaag gcaatacttc
     1741 acgccgacca ggatctccgg acctcgtagc gactgccccg ggccagcgtc accgactcga
     1801 ccagcgcgaa gccgtcgatc ttccagggaa tcggatccag cgcgggcatg cctttcggct
     1861 cgcgcgcgtc gcgtaggagc gtcacgtgcg acacgaaggg cttggcgtcg aaaccgatcc
     1921 gcgatttcgc aagcgcctcg cgcagccccg agacgagcgc cgccagctcc ggcgggacga
     1981 gcgaggcgcc cgcccaggcg atgcggttgt gcttccagta ccccggccgg tcgagcacca
     2041 gcgatgcaag cggcgccgcg acctcgcctg cggcgcgccc gacttccgcg a
//
LOCUS       DASPZD010000478         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_307719, whole genome shotgun sequence.
ACCESSION   DASPZD010000478 DASPZD010000000
VERSION     DASPZD010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307719"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VEM33_09290"
     CDS             complement(<1..262)
                     /locus_tag="VEM33_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52062.1"
                     /translation="MKSAERKRNVYVGAAFVSAIVALGACQALLENRAAAPGPTVQAP
                     MFEVDPLWPKPLPNHWLLGWTIGVWVDEQDHVWVIHRGAGGLH"
     gene            302..1702
                     /locus_tag="VEM33_09295"
     CDS             302..1702
                     /locus_tag="VEM33_09295"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HYS52063.1"
                     /translation="MLDAAKTVVNTRMIVWWVAALIWAMPSPAAAHDIPNDVTVHAFV
                     KPQGERLHLLVRVPLKAMRDVDYPRRGAGLLDLALADAALRNAATLWISDNVELYEND
                     SRLGLPRVADARVSLESDKSFASYEQALAHLAGPRLPSNMELYWEQGLLDVLFEYPIV
                     SDRSDFSIRPRLERLGLRTTVALRFLPAAGGVRAFDLHGDPGLVRLDPRWHQAAWRFV
                     ESGFLHILEGTDHLLFLLCLVIPFRKFGQLVLLVTAFTVAHSITLIASAYNLGPDALW
                     FPPLIETLIAISILYMAIENVLGSNVTRRWMITFGFGLVHGFAFSFALRETLQFAGSH
                     LLTSLLSFNLGVELAQLLVLALLVPALEILFRFLVAERLGTIILSVLVGHTAWHWMTE
                     RGERLGKFPWPALDASSLAVASRWLMGVLIVAASPASRAARCASGRDAGSSDRPRSPS
                     KGYTYEEEPIGAGRKG"
     gene            1705..1965
                     /locus_tag="VEM33_09300"
     CDS             1705..1965
                     /locus_tag="VEM33_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52064.1"
                     /translation="MTKRKKANGYARPVRTKRDYERAASVAKRLSSQADRDSAAELRL
                     QSLLRQLDKFDELEEEAGADDPADYPYSGPRRRWSDSESGEN"
     gene            complement(1969..>2190)
                     /locus_tag="VEM33_09305"
     CDS             complement(1969..>2190)
                     /locus_tag="VEM33_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52065.1"
                     /translation="MDAAIARAVEQLTGAGAASAIGNRRALRIAQEPFDLFRNYFALY
                     ALEQAYCHFSPALIENLERNWNAKSRKLG"
BASE COUNT          330 a          753 c          701 g          406 t
ORIGIN      
        1 cgtgcagccc ccccgctccg cgatgaatca cccagacgtg atcctgctcg tcgacccaca
       61 ccccgatggt ccagccgagc agccagtgat tcggcagggg cttcggccac agcggatcca
      121 cttcgaacat cggcgcctgg acggtgggtc ccggcgcggc ggccctgttt tccaacaagg
      181 cctggcaggc tccgagcgcg acaattgctg aaacgaatgc cgcgccgaca tagacgttgc
      241 gcttccgctc cgcgcttttc attgactccc ccttttccgt tgtaagctcg agctgccctc
      301 tatgctcgat gctgcgaaaa ccgttgtcaa cacccgaatg atcgtctggt gggtggcggc
      361 gctgatctgg gcgatgccgt cgcccgcggc cgcccatgac atcccgaacg acgtgacggt
      421 gcatgccttc gtcaagccgc agggagagcg gctgcatctt ctcgtccgcg tgccgctgaa
      481 ggccatgcgc gacgtggact atccgaggcg cggcgcgggc ctgctcgacc tcgcgctcgc
      541 cgacgcagcg ctccggaacg cggcaacgct gtggatttcc gacaatgtcg agctctacga
      601 aaacgacagc cgcctcggtt tgccgcgggt cgccgatgcc cgcgtgtcgc tcgagtccga
      661 caagtccttc gcctcctacg agcaggcgct cgcacacctc gccggcccgc ggctgccgag
      721 caacatggag ctgtactggg agcagggact gctcgacgtc ctgttcgagt atccgatcgt
      781 ctcggatcgt tccgacttct cgatccggcc ccggctcgag cggctcggcc tgcgaaccac
      841 cgttgcgctg cgcttcctgc ccgcagccgg cggcgtgcgc gccttcgacc tgcacgggga
      901 tccgggcttg gtcaggctcg acccgcgctg gcaccaggcc gcctggcgct tcgtcgagtc
      961 gggtttcctc cacatcctgg aggggaccga ccatctgctg ttcctgcttt gcctggtgat
     1021 accgttccgg aagttcgggc agctcgtcct gctggtcacc gcgtttacgg tcgcgcactc
     1081 gatcaccctt atcgcttccg cgtacaacct cgggcccgat gccctgtggt ttccgccgct
     1141 catcgaaacg ctgatcgcga tttccatcct gtacatggcg atcgagaacg tcctcggcag
     1201 caacgtcacg cgccgctgga tgatcacctt cgggttcggg ctggtccacg ggttcgcttt
     1261 ctcgtttgcg ctccgcgaga cgctgcaatt cgccggatcc cacctgttga cctcgctcct
     1321 gtcgttcaat ctcggtgtcg agctcgctca gctgctcgtg ctcgcgctcc tcgtccccgc
     1381 gctggaaatc ctgttccggt tcctggtcgc ggagcgcctg ggaacgatca ttctatcggt
     1441 gctggtgggc cacacggcct ggcattggat gaccgagcga ggggagcgcc taggcaagtt
     1501 cccgtggccg gcgctcgacg cctcgtcgct ggcagtcgcc agccgttggc tcatgggcgt
     1561 gctcatcgtc gcggcctcgc ctgcttcgcg cgcggcgcgt tgcgcgagcg ggcgcgacgc
     1621 cggcagcagc gatcgacccc ggtccccaag taaaggctac acttacgagg aagagcccat
     1681 cggggccggc aggaagggat agtcatgacc aagcgaaaga aagccaacgg ttacgcgcgg
     1741 ccggtgagaa cgaagcgcga ctacgaacgc gcggctagcg tggccaagcg cctctcgagt
     1801 caggccgacc gggattccgc cgccgagctg cgcctgcaat cgctgctccg gcaattggac
     1861 aagttcgacg aactggagga ggaggcgggc gcggacgacc cggcggacta tccctattcg
     1921 ggaccccgca ggcgctggtc ggacagcgag tccggcgaga actgacgctc agcccagctt
     1981 gcggctcttc gcgttccagt tccgttcgag gttttcgatc agcgccgggc tgaagtggca
     2041 gtaggcctgc tcgagcgcgt acagggcgaa atagttccgg aacaggtcga agggctcctg
     2101 cgcgatgcgc agcgcgcggc ggttgccgat cgcgcttgcc gcgcccgcgc cggtgagctg
     2161 ctcgaccgcg cgcgcgatcg cggcgtccat
//
LOCUS       DASPZD010000479         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_899896, whole genome shotgun sequence.
ACCESSION   DASPZD010000479 DASPZD010000000
VERSION     DASPZD010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899896"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..694)
                     /locus_tag="VEM33_09310"
     CDS             complement(5..694)
                     /locus_tag="VEM33_09310"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HYS52066.1"
                     /translation="MHFADQAFIAETTAKMLLEVKAVLFFEDKPFVFTSGWASPVYID
                     CRKLIFYPRLRSQLVDFAVATLARDVGFEQFDVVAGGETAGIPYAAWIAERLALPMQY
                     VRKKPKGFGRNAQIEGDIREGARTLLVEDLTTDGRSKINFCKALREAGAIVEHVFVNF
                     YYDIFPESKKTLAELNVRLHHLATWWDVLALVKKGGYLEPAQIGEVEKFLHAPAQWSA
                     AHGGVSAFPSS"
     gene            757..1386
                     /locus_tag="VEM33_09315"
     CDS             757..1386
                     /locus_tag="VEM33_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52067.1"
                     /translation="MVRELTIIAAAALIIASLAAHSQSTPSQLTYRCVGKDGKKYYGS
                     TVPTPCLGLPIEQLNTSGMVVKRINPEGDEKERAAKEAEAKKKREDDAARREAVRRNS
                     ALLATYTSAQDIDEARSRALADNQKAVKDVELRIDAIKKRQASYDKELELYKGDNKPP
                     ARLQDDIRDAEIDLKAQENLLAAKKKDIDTINARYDQDKQRYLELTGRR"
     gene            complement(1388..>2640)
                     /gene="gatB"
                     /locus_tag="VEM33_09320"
     CDS             complement(1388..>2640)
                     /gene="gatB"
                     /locus_tag="VEM33_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYS52068.1"
                     /translation="FGLAVKGQINRRSIFARKNYFYPDLPKGYQISQYELPIVAGGEV
                     KFFVDGEERRVRLTRAHLEEDAGKLLHELFQDMTGVDLNRAGTPLLEIVSEPELRGAK
                     EAVAYARALHGLVMWIGICDGNMQEGSFRCDANVSVKKPGEKQLGTRCEIKNLNSFRF
                     MERAIEFEARRQIELLEEGGKVEQETRLYDPDRDETRSMRTKEDAQDYRYFPDPDLLP
                     LVLREEQIRKIETEMPELPDATRERFARQYALPAYDAGVLTASRAAADYFEALVKAAG
                     VDAKLCANWQTGALSAALNEADLQVSESKVSPASLAGLLKRIADGTLSGKMAKEVFDA
                     MWAGEGAADAIIEKRGLRQISDAGAIDRLIEEVLAANPKQVEDYRAGKEKAFNSLVGQ
                     VMKASRGKANPQQVNEVLRRKLSG"
BASE COUNT          486 a          890 c          802 g          462 t
ORIGIN      
        1 atcctcaaga cgatgggaaa gcggagaccc cgccatgcgc cgccgaccac tgcgccggcg
       61 cgtgcagaaa cttctcgact tcgccgatct gcgccggctc gaggtagccg cccttcttca
      121 ccagcgccag cacgtcccac cacgtggcga gatgatgcag gcgcacgttg agctcggcga
      181 gcgtcttctt cgactcgggg aagatgtcgt agtagaagtt gacgaacacg tgctcgacga
      241 tcgctcccgc ctcgcgcagc gccttgcaga aattgatctt cgagcgcccg tcggtggtga
      301 ggtcctcgac cagcagcgtg cgcgccccct cgcggatgtc gccctcgatc tgcgcgttgc
      361 ggccgaagcc cttcggcttc ttcctcacgt attgcatcgg cagcgcgagg cgctcggcga
      421 tccacgccgc atacggaatc cccgccgtct cgccgccggc gaccacgtcg aactgctcga
      481 agccgacgtc gcgcgcgagc gtcgcgacgg cgaagtcgac caattggctg cggaggcgcg
      541 gataaaagat gagcttgcgg cagtcgatgt acaccgggct cgcccagccg gaggtgaaga
      601 cgaaaggctt gtcctcgaaa aacagcaccg ccttcacctc gagcagcatc ttggcggtgg
      661 tctcggctat gaacgcctgg tcggcgaagt gcatgagcgg cgcgaatttt aacaggttag
      721 aatccgccta cctcgcgctc cacggagaat catgcgatgg tacgggagct aacaataatc
      781 gcggcggcgg cgctgatcat cgcatcgctc gcagcgcact ctcaatccac gcccagccag
      841 ctcacctacc gctgcgtcgg caaggacggc aagaaatact acggctcgac ggtgcccacg
      901 ccgtgcctcg gcctgccgat cgagcagctc aatacttccg gcatggtcgt caagcgaatc
      961 aacccggagg gcgacgagaa agagcgcgcg gcgaaggaag ccgaggcgaa gaaaaagcgc
     1021 gaggacgacg ctgcccgccg ggaagccgtc cgccgcaaca gcgccctgct cgccacctac
     1081 accagcgcgc aggacatcga cgaagcccgc tcgcgcgctc ttgccgacaa ccagaaggcg
     1141 gtcaaggacg tcgagctgcg catcgatgcg atcaagaagc gccaggcgag ctatgacaag
     1201 gagctcgagc tctacaaggg cgacaacaag ccgccggcgc ggctccagga tgacatcagg
     1261 gacgcggaaa tcgacctgaa ggcgcaggaa aacctgctcg cggcgaagaa gaaggacatc
     1321 gataccatca acgccaggta cgaccaggac aagcagcgct acctggagct gaccggccgc
     1381 aggtagctca gcccgagagc ttgcggcgca gcacctcgtt cacctgctgc ggattggcct
     1441 ttccccggct cgccttcatc acctgtccga ccagcgagtt gaaggctttc tccttcccgg
     1501 cgcggtaatc ctcgacctgc ttcggattcg ccgcgaggac ctcctcgatc agcctgtcga
     1561 tcgcaccggc gtcgctgatc tgcctcagtc cgcgcttttc gatgatcgcg tccgccgcgc
     1621 cctcgccggc ccacatggcg tcgaacacct ccttcgccat cttgcccgag agggtgccgt
     1681 cggcgatgcg cttcagcaac ccggccaggg acgcggggct tactttggat tcgctcacct
     1741 ggagatccgc ctcgttgagc gctgcgctca gcgcgccggt ctgccagttg gcgcacagct
     1801 tcgcatcgac gccggccgcc ttgaccagcg cctcgaaata gtcggccgcg gcgcgcgatg
     1861 ccgtcagcac gccggcgtcg tacgcgggca gcgcgtattg ccgcgcgaag cgctcgcgcg
     1921 tcgcatccgg caactctggc atttcggttt caattttcct gatctgctct tcgcgcagca
     1981 ccaacggcaa aagatcggga tccgggaagt agcgatagtc ctgggcgtct tccttcgtac
     2041 gcatggaacg cgtctcgtcg cggtccggat cgtaaaggcg cgtttcctgc tccactttgc
     2101 cgccttcctc gagcagctcg atctggcgcc gcgcctcgaa ttcgatcgcg cgctccatga
     2161 agcggaacga attgaggttc ttgatctcgc agcgcgtgcc cagttgtttc tctcctggct
     2221 tttttactga aacgttcgcg tcgcaacgaa acgagccttc ctgcatgttc ccgtcgcaga
     2281 tgccgatcca catcacgagc ccgtgcagcg cgcgcgcgta cgcgaccgcc tcctttgcgc
     2341 cgcgcagctc gggctcggag acgatttcga gcagcggggt gccggcacgg ttgaggtcga
     2401 cgccggtcat gtcctggaac agctcgtgca gcagcttgcc ggcgtcttcc tccagatggg
     2461 cgcgcgtgag ccgcacgcgc ctttcctccc cgtcgacaaa aaacttcact tcccctccgg
     2521 cgacgatcgg cagctcgtac tggctgatct ggtagccctt ggggaggtcg ggatagaaat
     2581 aattcttgcg cgcgaagatg gagcggcggt tgatctgtcc cttcacggcc aggccgaagc
//
LOCUS       DASPZD010000480         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1075228, whole genome shotgun sequence.
ACCESSION   DASPZD010000480 DASPZD010000000
VERSION     DASPZD010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075228"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VEM33_09325"
     CDS             <1..1615
                     /locus_tag="VEM33_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYS52069.1"
                     /translation="EVIFLIGANPTVNHPVAATWMKNAAKAGVKLIVADPRRGELARH
                     ATYNLQFKADTDVALLNAMLHVIVEEGLVNEAFVRDRTSGYEALAENVKKFSPEAMAP
                     ICGIDAKTIREVARLYATSKASIIMWGMGISQHVHGTDNARCLIALALSTGQIGKPGS
                     GLHPLRGQNNVQGASDSGLIPMFYPDYQRVITPEAKARFEKLWGMELDPNPGLTVVET
                     IHAAKKREIRGMYIMGENPAMSDPDVDHAREALATLDHMVVQDIFLTETAYLADVVLP
                     ATAWPEKVGTVTNTDRMVQLGRKAIEPPGEAKEDLWLINELAKRIGLPWNYTHPREVF
                     NEMRQCMDSIAGITWERLERDSSVTYPCEKEGDPGEPVVFVTHFPTTTGRGKFVPADL
                     ISADERPDDQYPMVLITGRQLEHWHTGAMTRRASVLDHIEPEPVASFHPLDLDKVGVK
                     PGDIVTVESRRGKIALYARADDGTPAGAVFIPFCYYEAAANMLTNPKLDPVGKIPEFK
                     YCAVKVTKGGPTPVHSSFGGGTLLAETAS"
     gene            complement(1677..>2073)
                     /locus_tag="VEM33_09330"
     CDS             complement(1677..>2073)
                     /locus_tag="VEM33_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52070.1"
                     /translation="TFTAKTLCLDNIFVAHPYLEGVDGIALDAAGNIWADPNERNAIV
                     VVTKKGEVLEIFRNEPNPTTNLRNTGPLETPTSPFLLGHKFCTANSDGNRRDNVPNAA
                     GEIAPGSGFQGKISCMDQALKIPGMRLPI"
BASE COUNT          390 a          665 c          669 g          349 t
ORIGIN      
        1 ggaagtgatc ttccttatcg gcgccaaccc gacggtaaac catccggtgg cggcgacctg
       61 gatgaagaac gcggcgaagg cgggcgtgaa gcttatcgtc gcggatcccc gcaggggcga
      121 gctcgcgcgc catgcgacct acaacctgca gttcaaggcc gacaccgacg tcgcgctcct
      181 gaacgcgatg ctccatgtca tcgtcgagga aggcctggtc aacgaagctt ttgtccgcga
      241 tcgtacttcg ggctacgagg cgctcgctga aaacgtgaag aaattttcgc cggaagcgat
      301 ggcgcccatc tgcggcatcg atgcaaaaac gatccgcgaa gtcgcacgcc tgtacgcgac
      361 ctcgaaagcc tcgatcatca tgtgggggat gggcatctcg cagcacgtgc acggcaccga
      421 caacgcgcgc tgcctgatcg cgctcgcgct ctcgactggc cagatcggca aaccgggctc
      481 gggcctgcac cctctgcgcg gccagaacaa cgtgcaaggt gcatcggact cagggctgat
      541 cccgatgttc tatcccgact accagcgcgt catcacgcct gaggccaagg cgcgcttcga
      601 aaagctctgg ggcatggagc tcgatccgaa ccctggcttg acggtagtcg agaccatcca
      661 cgcggcgaag aagcgcgaga tccgcggcat gtacatcatg ggcgagaacc cggcgatgtc
      721 cgaccccgac gtcgatcacg cgcgcgaggc gctcgccacg ctcgatcaca tggtggtgca
      781 ggacatcttc cttactgaga ctgcgtatct ggcagatgtc gtgttgcctg cgaccgcgtg
      841 gccggagaaa gtcggcacgg tcaccaacac cgaccgcatg gtgcagctcg gcaggaaggc
      901 gatcgagccg ccgggcgagg cgaaagagga cctctggctc atcaacgagc tcgccaagcg
      961 aatcggcctg ccgtggaact acacgcaccc gcgcgaggtg ttcaacgaaa tgcgccagtg
     1021 catggactcg atcgcgggca tcacctggga gcgcctcgag cgcgattcgt cggtgaccta
     1081 tccgtgcgaa aaggaaggcg accccggcga gcccgtcgtt ttcgtcacgc atttccccac
     1141 gaccacgggc cgcggcaagt tcgttcccgc ggacctgatt tccgccgacg agcgcccgga
     1201 cgaccagtac ccgatggtgc tcatcaccgg caggcagctc gagcactggc acaccggcgc
     1261 gatgacgcgc cgcgctagcg tgctcgatca catcgagccc gagccggtcg cgtctttcca
     1321 tccgctcgac ctcgacaagg tgggcgtcaa gcccggggac atcgtcacgg tggaatcgcg
     1381 ccgcgggaag atcgcgctct acgcgcgcgc cgacgacggc actccggcgg gggctgtgtt
     1441 catcccgttc tgctactacg aagcggcggc gaacatgctc accaatccga agctcgaccc
     1501 ggtcggcaag atcccggagt tcaagtactg cgcggtgaaa gtcaccaaag gcgggccgac
     1561 gcccgtgcac tcgagcttcg gcggcggaac gctgctcgcc gaaacggcga gctgatcgga
     1621 aacagggcga aaaaaaagcc ccggtcgccc ggggcttttt atttgttgcg cttcggtcag
     1681 atcggcaggc gcatgccggg gatcttcagc gcttggtcca tgcacgagat cttgccctgg
     1741 aagccggacc cgggcgcgat ctcgccggcc gcgttcggga cgttgtcgcg ccggttgccg
     1801 tccgagttgg cggtgcagaa cttgtggccg agtaggaagg ggctggtcgg agtctccagc
     1861 ggcccggtgt tgcgcaggtt cgtggtcggg ttcggctcat tgcggaatat ctcgaggacc
     1921 tcgcccttct tcgtcaccac gacgatcgca ttgcgctcgt tcgggtcggc ccagatgttg
     1981 ccagccgcgt cgagcgcgat gccgtccacg ccttcgagat acgggtgcgc gacgaagata
     2041 ttgtcgagac acagcgtctt cgccgtgaag gtc
//
LOCUS       DASPZD010000481         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_286551, whole genome shotgun sequence.
ACCESSION   DASPZD010000481 DASPZD010000000
VERSION     DASPZD010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286551"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VEM33_09335"
     CDS             complement(<1..1006)
                     /locus_tag="VEM33_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYS52071.1"
                     /translation="METQVLVVGGGPVGLTLAIDLGRRGVRCTLIEQKQAPQFLPKME
                     RCNARTMEIFRRMGIADKVRAAGLRGEIPMDVFIILSLAEPPLLHLPYPSVERARGEI
                     RACSDGSMPLEPYQLISQYTLEPLLKSIAEGLPSVKVRYGCRLESFTQDESGVTARVK
                     HANGTGGVIGAPYMVGCDGGGSTVRKQLGIKLRGEHNLLELRQALYRCDELFERIPIG
                     KGPGRGRHYHVADGNNGFLIMQDSTKHFTLHAVVENDDDMKAMFERTVAIPVKYEMLT
                     CAPWRQNLLLADGYRDGRVFLAGDAVHLVIPTGGLGMNSGVGDAIDLSWKLAATLQGW
                     G"
     gene            complement(1112..1939)
                     /locus_tag="VEM33_09340"
     CDS             complement(1112..1939)
                     /locus_tag="VEM33_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52072.1"
                     /translation="MSSRDPAEIDACTGLLEPGTAVYISMPPGQTYHGNVALATRLKR
                     AGFHPVPHIAARRIASRDALAEYLARAAGEAGVDSALVIAGDSDRPSGPFDSSRALLE
                     TGLFQRHGVVRIGVAGYPDGHPKIAEAALGTALAEKRALARGAGLDLQVVTQFCFESE
                     PILAWAAKMKGHGLPMRVGLAGPASLPRLLRFAALCGIGNSVRALKARPRAITRLLVE
                     AGPEAVVRNIARPGGAAIAGVHFFCFGGLLRTARWLRAVREGRFELTPDGGFRVGAA"
     gene            complement(2056..3474)
                     /locus_tag="VEM33_09345"
     CDS             complement(2056..3474)
                     /locus_tag="VEM33_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52073.1"
                     /translation="MTAEQLLSEIADYCRRVGMAESTFGRRAVNDGKLVGRLRIGGRV
                     TMETVERVRTFMSEHRSVESRGLVRLRSSAGSLASGGGAVLDQPKIAASTEARHESKT
                     NFRFYDNRQKYLLFVSTCSEKWVVAQRVGMELANIHPRPPAVRVFDAGVGDGTVLARV
                     MRAMHQRYPTMPFHIVGKEISLEDVRLALDRMPDRFFEHPATVLVMTNLYYSEAPWLT
                     PKSVTAATSLVWKEVALTGATSSEFEQQIIDLQPFLNEHWRASVSKTSGNPAYAKPVV
                     LVFYREDHKFLLDQVLPRRGAARADYDLVIASQPYRARASAEFKASKVLAPLARSLAP
                     GGRMIAIHSHGRDPGLEIVQRVWPGEDPFQTDRHSLLKATRAALGADGRDLNFNAYAD
                     NRSIFRYEMHTLPEEISDAIGTSTLLAAWNAAVYVAQIEDQRLGEVMGDRGYLEATQE
                     VLRRHKGLWFLDESYVISRKRD"
     gene            3909..>4185
                     /locus_tag="VEM33_09350"
     CDS             3909..>4185
                     /locus_tag="VEM33_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HYS52074.1"
                     /translation="MAAVLQTKLAADRKAFYGKIDKANVTPLWEVLHSIITATPSTPC
                     KPHLWKWDQMWPWIQEAGRLISAQEAERRVLVLENPGLRGRSSITHSL"
BASE COUNT          733 a         1371 c         1325 g          756 t
ORIGIN      
        1 cgccccatcc ctgcagggtt gccgcgagct tccaggaaag atcgatcgcg tcgccgacgc
       61 cggaattcat gccgagaccg ccagtaggta tgaccagatg gaccgcgtcg ccggcgagaa
      121 acacgcggcc atcccgatag ccgtctgcta gcagcaggtt ctgccgccac ggagcgcagg
      181 tgagcatctc gtacttcacg ggaattgcga cggtgcgctc gaacatcgct ttcatgtcgt
      241 cgtcgttctc gaccacggcg tgcaaggtga agtgcttcgt cgagtcctgc atgatgagaa
      301 agccgttgtt accgtcggcc acgtgatagt ggcgccccct gccgggcccc ttgccgatgg
      361 ggatgcgctc gaacagctcg tcgcagcgat acagcgcctg gcgcaactcg agaaggttgt
      421 gctcgccgcg cagcttgatg ccgagctgct tgcgcacggt gctcccgccg ccgtcgcagc
      481 ccaccatata cggcgcgccg atcacgccgc ccgtgccgtt cgcatgcttc acgcgcgcgg
      541 tgaccccgga ctcgtcctgg gtaaacgatt caagccggca accgtagcgt accttcaccg
      601 agggcagccc ctcggcgatc gacttgagca gcggctccag cgtgtactgc gaaatgagct
      661 ggtacggttc cagcggcatg ctgccgtcgc tgcaggcgcg gatctcgccg cgcgccctct
      721 ccaccgaagg gtagggcagg tgcaggagcg gcggctcggc gagcgacagg atgatgaaca
      781 cgtccatcgg gatttcgccg cgcagtccgg cggcacggac cttgtcggcg attcccatgc
      841 gccggaagat ttccatggtg cgcgcattac atcgttccat cttcggcaga aactgcggtg
      901 cttgcttctg ctcgatcaag gtgcagcgca cgccgcgcct gccgagatcg atggcaagcg
      961 taaggccgac tgggccgccg ccgacgacca gtacctgtgt ttccatcgcc cgtattctgc
     1021 cttacccgcc gagggcggtg cggtgttagc tcagtggagc tccgctcact cggcctagtg
     1081 agctttatcc cgtaggtgat cgccgcagaa cttaagcggc cccgacccga aaaccgccgt
     1141 cgggggtcag ttcgaatcgc ccttcacgca ccgctcgcag ccagcgcgca gtcctcaaaa
     1201 ggcccccgaa gcagaaaaaa tgaactccgg cgatcgccgc accgcctggc cgcgcaatgt
     1261 tcctcacaac ggcctccggg ccggcttcca ccaggaggcg cgtaatcgcc cgcggtcgtg
     1321 ccttgagggc gcggaccgag tttccgatcc cgcaaagcgc cgcaaatcgc aacagccgcg
     1381 gcaggctcgc cgggcccgcg agcccgacgc gcatcggcag cccgtgccct ttcatctttg
     1441 cggcccatgc gagaatcggc tcggactcaa agcagaactg cgtcacgacc tgcagatcga
     1501 gacctgcccc acgggcgagg gctcttttct cggcgagcgc ggtcccgagg gccgcttccg
     1561 ctatcttggg gtgaccatcg gggtagcccg ctaccccgat gcgcacgact ccgtgacgct
     1621 gaaacaggcc ggtttcgagc agggcgcggc tcgaatcgaa cggcccgctc ggccggtcgc
     1681 tgtcgccggc gatcacaagg gcgctgtcaa ctccggcctc gcctgcagcg cgcgcaaggt
     1741 actctgcgag cgcatcgcgg ctggcgatcc ggcgcgccgc gatgtgggga accgggtgaa
     1801 agccggctcg tttcagccgc gtggccagcg ctacgtttcc gtggtaggtc tgtcccggcg
     1861 gcatgctgat gtaaactgcg gtccccggct ccagcagacc ggtgcacgcg tcgatctccg
     1921 ccggatcccg agacgacaat tcgagcgagg ctccggctag cagagccggg atgttccgga
     1981 gatcatcgcc ttgcgatggg ccgatgagtc gcgttgcggt gtcaggctcc acggtcaggt
     2041 ccgcttgcct gcgcctcagt cgcgcttgcg cgagatcacg tacgactcgt cgaggaacca
     2101 caggcccttg tggcgacgaa gcacctcctg cgtagcctcg agatatccgc ggtctcccat
     2161 gacctcgccc aggcgctgat cttcgatctg cgccacgtaa acggcggcgt tccacgcggc
     2221 gagaagagtc gaggtgccga ttgcgtcgct gatttcctcc gggagggtgt gcatctcgta
     2281 gcggaagatc gagcggttgt cggcgtaggc gttgaagttg aggtcgcggc cgtctgcgcc
     2341 gagcgcggcg cgggtggcct tcaggagcga gtggcgatcg gtctggaacg gatcttctcc
     2401 cggccagacg cgctgcacga tctcgagccc cgggtcccgg ccgtgggaat ggatcgcgat
     2461 catccgcccg cccggcgcca ggctccgagc gagcggtgca aggaccttgg acgccttgaa
     2521 ttcggccgag gcgcgggcac ggtagggctg cgacgctatg accaagtcgt agtcggcgcg
     2581 ggcagcgccg cggcgcggta acacctggtc gagaaggaac ttgtgatctt cccggtagaa
     2641 caccagcacc accggcttcg catacgcggg attgccgctc gttttgctca ccgacgcgcg
     2701 ccagtgttcg ttcaggaacg gctgcaggtc gatgatctgc tgttcgaact cgctggaggt
     2761 cgctcccgta agcgcgacct ctttccagac gagactcgtg gcggcggtga ccgacttcgg
     2821 cgtgagccac ggagcttccg agtagtagag attggtcatg acgagcaccg tcgccgggtg
     2881 ctcgaagaag cggtccggca tgcgatcgag cgcgagccgg acgtcctcca ggctgatttc
     2941 cttgcccacg atgtggaagg gcatcgtcgg atagcgctgg tgcatggcac gcatcacgcg
     3001 ggcgagcacg gtgccgtcgc cgacgccggc atcgaacacc ctgaccgcgg gaggccgcgg
     3061 atgaatattg gcgagttcca tgccgacgcg ctgcgcgacc acccacttct cgctgcaggt
     3121 gctgacgaac agcaaatact tctggcggtt gtcgtaaaag cggaagttgg tcttcgactc
     3181 gtgtctcgct tcggtggacg cggcgatctt gggctgatcc aggaccgcgc ccccgccgct
     3241 cgccaggctc ccagccgagg accgcagccg aaccaagccg cggctctcga ccgaccggtg
     3301 ctccgacatg aacgtgcgca cgcgctccac ggtttccatg gtcacgcgcc caccgatgcg
     3361 cagccggccg acgagcttgc cgtcgtttac ggcgcgccgc ccgaaggtcg actcggccat
     3421 gccgacacgc ctgcagtaat ccgcaatctc gctcagcaac tgctcagccg tcacgtcatt
     3481 tcctcctctt tcctcccgtc gagcgactgg ccgcttcggc gtaattacag gatgcctggg
     3541 cagcgtgggt caaccacaat tgtgcccatc gcgaaagtgg gaatgatagt gggacaaatc
     3601 ccactgggcc tgtgtcagcg cgcactccgg cgcaatgata caaataattt atttattatc
     3661 aaaaagttat tgtagaaacc ccttgagcta ttccaagtag gccctctgaa cctgaccttc
     3721 atggcggagg aaagcgccta caagtatcgg cgctatcata cgagaaaagt gggctttcgc
     3781 ccactttttc aggacgctcg cctcatgccc tcggctccat cgcctgggct ggcccgcgag
     3841 gttgccgtga tcgtctgctt tagcggaaaa tcgttcctca accccgtcgg gaatctctgg
     3901 aggagcggat ggccgcggtt ctgcaaacca agcttgccgc agatcgcaag gcgttttacg
     3961 gcaagatcga caaggccaat gtcacgcctt tgtgggaggt gttgcacagc atcatcaccg
     4021 cgacccccag cacgccctgc aagcctcatc tctggaaatg ggatcagatg tggccgtgga
     4081 tccaggaagc cggccgcttg atcagcgctc aggaggccga gcgacgcgtt ctggtgctcg
     4141 agaaccccgg gctgaggggt cgctcgagca ttacccacag ccttt
//
LOCUS       DASPZD010000482         9542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_420084, whole genome shotgun sequence.
ACCESSION   DASPZD010000482 DASPZD010000000
VERSION     DASPZD010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420084"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VEM33_09355"
     CDS             <1..717
                     /locus_tag="VEM33_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HYS52075.1"
                     /translation="RAKRLCAALLLAGTLAGCATSGGNPADPLEPFNRAMFSFNDAVD
                     GVVMKPVAKGYRSVVPGFLRTGISNFFSNLEDVWISVNDVLQGKFQQGLEDFTRVFFN
                     STFGIAGVFDFASDVGLQKHSEDFGQTLGWWGVGSGAYLVLPILGPSTVRDGFGFLVD
                     TQADPVWRVGNVPARNTAVGVRFIARRAELLDATSVVEQAALDKYSFVRDAWMQRRRN
                     LVYDGDPPREPEEPDDEPTK"
     gene            734..1357
                     /locus_tag="VEM33_09360"
     CDS             734..1357
                     /locus_tag="VEM33_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYS52076.1"
                     /translation="MKIPFYALFALLIPLAHAQQPVAPDVLVKSITEEVAGILKQDKD
                     IQAGDRKKAADLIETKIVPHFNFIRMTRIAMGRNWRLASPEQQKVLADEFKTLLVRTY
                     STALSNYRDQQIDYKPLRAKPDDTEVTVRSEVKQSGSSQPVAIDYEMEKTPNGWKVYD
                     VKVGGVSLVTTYRDTFAGEVRERGVDGLIKSLVAKNRQPERSKAGKT"
     gene            1354..1641
                     /locus_tag="VEM33_09365"
     CDS             1354..1641
                     /locus_tag="VEM33_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYS52077.1"
                     /translation="MIRREGDSLILEGPVTLDTVPGLVGTSEEHLRQGVRVVDFGSVT
                     DVDSAAVALALEWLRRAAESGRGLRLANLPAAMQNLAKLYGVSELLQSASS"
     gene            1666..2562
                     /locus_tag="VEM33_09370"
     CDS             1666..2562
                     /locus_tag="VEM33_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYS52078.1"
                     /translation="MPAAIEVRDVHKRFGELQALAGIDLEIRQGEFFGLLGPNGAGKT
                     TLISVLAGLARADRGSARVMGHDVRTDYRAARRSLGVVPQELVFDPFFTVRETLRFQS
                     GYFGVRNNGAWIDEVMRHLDLASKAEVNMRALSGGMKRRVLVAQALVHKPPVIVLDEP
                     TAGVDVELRQALWQFIQRLNRDGHTIVLTTHYLEEAEALCERIAMLKQGRVVALDSTS
                     NLLKRVHHLFLRLRLDGELPGELRPLEHGSTAGAHILRLKGYEDVEQTLACIRRAGCR
                     IEEMELMQPDLEDAFVDVMQRS"
     gene            2559..3314
                     /locus_tag="VEM33_09375"
     CDS             2559..3314
                     /locus_tag="VEM33_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYS52079.1"
                     /translation="MSGFPTLLYKEVLRFWKVSTQTIGAPVLTAVLFLFIFSHVMEGR
                     IRMYDTVPYAGFLVPGLVMMSVLQNAFANSSSSLVQSKMTGNIIFVLLPPLAYWEFFA
                     AYVLAAAARGMVVGAAVLLVTLPFVEIEFRHPLWIVAFAFFASAVLGSLGMIAGIWAE
                     KIDQVAAFQNFFVMPLTFLSGVFYSIHSLPPFWQGLSHFNPFFYMIDGFRYGFFGVSD
                     VHPFRSLTVVTAFLGVVSAMTLAMIKSGYRLRH"
     gene            3316..3552
                     /locus_tag="VEM33_09380"
     CDS             3316..3552
                     /locus_tag="VEM33_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HYS52080.1"
                     /translation="MVTPEQVQSYIEKGLQCSAVRVVGDGEHFEALVVSEAFRGKSRV
                     QRHQLVYAALGERMRGEIHALSMRTLTPEEAADG"
     gene            3545..4798
                     /gene="murA"
                     /locus_tag="VEM33_09385"
     CDS             3545..4798
                     /gene="murA"
                     /locus_tag="VEM33_09385"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYS52081.1"
                     /translation="MDKLIIQGGVPLSGEIRVSGAKNAALPLMCAALLTSEQLTLSNV
                     PHLRDVSTMLRLLAQMGVEVSLDDRLGLSLRAERLSDPLAPYDLVKTMRASILVLGPL
                     LARCGEARVSLPGGCAIGTRPVDLHVKGLAALGAEISVEQGYISAKAKRLKAARILMD
                     LVTVTGTENLMMAACLAEGTTTLENAAREPEVVDLAQCLSAMGARIKGAGTDVITIEG
                     VARLRAARHRIMADRIETGTFLAAAAATGGCVTLHGANAGILDAVLEKLREAGAEVQT
                     GDGRLSLTMRGRPKGVNVRTAPYPAFPTDMQAQLMALDAIAEGTAVITETIFENRFMH
                     ALEMQRLGADIEISGNTAVVHGVAKLQGATVMATDLRASASLVIAGLVAEGETRVDRI
                     YHLDRGYECIEEKLAQLGARIKRVH"
     gene            4908..5567
                     /gene="hisG"
                     /locus_tag="VEM33_09390"
     CDS             4908..5567
                     /gene="hisG"
                     /locus_tag="VEM33_09390"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYS52082.1"
                     /translation="MADSTVITLALSKGRIFDETLPLLAAAGIETLDNPDTSRKLILR
                     TSRSDLRLLIVRAPDVPTYVQYGASELGIAGKDVLLEHGGEGLYQPLDLGIASCRMMV
                     AVPEGFDYAGAVRQGARLRVATKYMQTAREHFAAKGVHVDLIRLYGSMELAPLVGLAD
                     AIVDLVSSGDTLRANRLHVVEEIRPISARLIVNQAALKLKRDAIEPLLDQFTKAAGKH
                     A"
     gene            5560..6879
                     /gene="hisD"
                     /locus_tag="VEM33_09395"
     CDS             5560..6879
                     /gene="hisD"
                     /locus_tag="VEM33_09395"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYS52083.1"
                     /translation="MLEIARLSTTQSDFDARLKALTAFDTTFDQKVEDDVAAILAGVS
                     TRGDAAVLEYTERFDRIKVARVADLELPRRELEKARSGLNGGEREALEQAAVRIRKYH
                     ERQAAKSWQFTEADGTTLGQRVTPLERVGMYVPGGLAAYPSSVLMNAIPARIAGVREL
                     IMCVPTPDGKSNPLVLAAAAIAEVDRVFTVGGAQAIAALAYGTQTIPQVDKIVGPGNA
                     WVVAAKRRVFGTVGIDMISGPTEILVICDGSTEPDWIAMDLFSQAEHHETSQAILVSP
                     DKAFLERVDESIGRLLAGMPRAEIIEKSLARRGALIKVKNLDEACDIANRIAPEHLEL
                     SVAEPKKWLKRIRHAGAIFLGRYSSEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQK
                     RSSIIGVSRKGAQKLGKLAMTLANSEGFRAHARSAELRLGKTLKEKP"
     gene            6876..7952
                     /gene="hisC"
                     /locus_tag="VEM33_09400"
     CDS             6876..7952
                     /gene="hisC"
                     /locus_tag="VEM33_09400"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYS52084.1"
                     /translation="MSAEPEKVIRDEIRELSAYHVQPAQGMVKLDAMENPYRLPEPLR
                     REIGELAANAEINRYPDPTAPALMRRLREVMGIGPEYDVLLGNGSDEIIHIIMQCVAR
                     PGAVVLAPRPAFFMFSQYARFAGLPYFGVLLNPDFTLDTGAFLHAIAEHQPAVVFIDY
                     PNNPSGTLFSDEGIARIIAAAPGLVVIDEAYNPFARKTFMKRLAEFPNLVVMRTLSKL
                     GLAGIRLGYAVGRPEWIGEFNKVRPPYNVNVLTLLVAERVLAHYDVLDAQARSINQER
                     ARLGGELRKLPKVTVFPSDANFILVRVPDAHRVFEGMRRRGVLIKNLHTGVPLLDHCV
                     RLTIGTPEENRRCLAAFAEALAEG"
     gene            7987..8580
                     /gene="hisB"
                     /locus_tag="VEM33_09405"
     CDS             7987..8580
                     /gene="hisB"
                     /locus_tag="VEM33_09405"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HYS52085.1"
                     /translation="MPTRAATADRKTTETQIALSVNVDGSGRWSIATGIPFFDHMLEQ
                     VARHAVLDLDIRAKGDLQVDAHHTVEDIGITLGQAVARAVGDKKGIRRYGHAYVPLDE
                     ALSRVVIDFSGRPGLEYHVQFKRALIGEFDVDLVHEFFQGFANHAQVTLHVDNLRGDN
                     AHHQCETMFKAFARALRMALEPDPRAAGTVPSTKGTL"
     gene            8605..9240
                     /gene="hisH"
                     /locus_tag="VEM33_09410"
     CDS             8605..9240
                     /gene="hisH"
                     /locus_tag="VEM33_09410"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYS52086.1"
                     /translation="MRVAVVDYGMGNLHSVSKAVEHVAPHASVEVTSDPAHVAGADRV
                     VFPGQGAMPDCMREMDARGLRPAVLDAARSRPFLGICIGLQMLFERSDEGDVAGLALL
                     PGAVRSFPAEAMVDDEGRKLKVPHIGWNRVKQAAPHALWSGIEDGSRFYFVHSYFPQT
                     GEAALVRGTTDYGVPFTCAVARDNIFAVQFHPEKSDTAGLRLLANFLNWNP"
     gene            9295..>9542
                     /locus_tag="VEM33_09415"
     CDS             9295..>9542
                     /locus_tag="VEM33_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HYS52087.1"
                     /translation="MLIIPAIDIKDGHCVRLKQGKMNEATVFSEDPVGMAKHWKAQGA
                     RRLHLVDLNGAAAGKPKNESVIRAILQALGDSPPVQLGG"
BASE COUNT         1690 a         3012 c         3076 g         1764 t
ORIGIN      
        1 agggccaaga ggctgtgcgc ggcgctgctc ctcgccggaa cgctggcggg ctgcgccact
       61 tcggggggca atccggcgga ccctctcgag ccgttcaatc gcgcgatgtt cagtttcaac
      121 gatgcggtgg acggagttgt gatgaagccg gtggccaagg gttaccgcag cgttgtgccg
      181 ggattcttgc gtaccggcat ttccaatttt ttttccaatc tcgaggacgt ctggatttcg
      241 gtgaacgatg tgctgcaggg caagttccag cagggcctcg aggatttcac tcgggtcttt
      301 ttcaattcga ctttcgggat cgccggcgtt ttcgacttcg cgtccgacgt tggattgcag
      361 aagcacagcg aggatttcgg gcagacattg ggctggtggg gcgtcggcag cggcgcgtac
      421 ctggttctgc cgatcctggg tccgtccact gttcgcgacg gattcggatt cctggtcgat
      481 acccaggccg acccggtgtg gcgagtcggc aatgttcccg cgcgcaacac ggccgtcggg
      541 gtccggttta tcgcgcgcag ggccgaattg ctcgatgcga ccagcgtggt cgagcaggcc
      601 gcgctcgaca aatactcgtt cgtgcgcgac gcctggatgc agcgccgccg caacctcgtc
      661 tacgacggcg atccgccgcg ggagcccgag gagcccgatg atgaacccac caagtgacca
      721 aaatcctatg gccatgaaaa tccctttcta cgcgctcttt gcattgctga tccctctcgc
      781 gcacgctcag cagccggttg cgcccgacgt cctggtgaaa tcgatcaccg aggaggtcgc
      841 cggcatcctg aagcaggaca aggacattca ggcaggcgac aggaaaaagg ccgccgatct
      901 gatcgagacc aagatcgtcc cgcacttcaa cttcatccgc atgacccgga tcgccatggg
      961 acgcaactgg cggctcgcct cccccgagca gcaaaaggtg ctcgccgacg aattcaagac
     1021 gctgctggtt cggacctatt cgaccgcgct ctctaactac cgcgaccagc agatcgatta
     1081 caagcccctg cgcgccaagc ccgacgatac cgaggtcacc gtgaggtcag aggtcaagca
     1141 atcgggctcg tcccagccgg tcgccatcga ctacgagatg gaaaagactc caaacgggtg
     1201 gaaagtgtat gacgtcaagg tcggcggggt gagcttggtt acgacctacc gcgacacctt
     1261 cgccggcgaa gttcgcgagc gcggcgtgga cggcctgatc aaatcgctcg tcgcgaaaaa
     1321 ccgccagccc gagcgctcga aagcgggcaa gacgtgatcc gtcgagaggg cgactcgctc
     1381 atcctcgaag gcccggtgac gctggatacc gtaccggggc tggtcgggac ttcggaggag
     1441 cacttgcggc agggcgtgcg cgtggtcgac ttcggcagcg tgaccgacgt cgattccgcc
     1501 gcggtagccc ttgcactgga gtggctgcgt cgggccgcag agagcgggcg cggattgcgc
     1561 ctcgccaatt tgcccgctgc gatgcagaat ctcgccaaac tctacggagt ctccgagctg
     1621 ctacagtcgg cttcgagctg aaccttcgcg cgggcgacca ccggcatgcc tgccgcgatc
     1681 gaagtccgcg acgtccacaa gcgcttcggc gagctgcagg cgctcgcggg gatcgacctc
     1741 gagatccggc agggagagtt tttcgggttg ctcggaccca atggagccgg caagaccacc
     1801 ttgatcagcg ttctcgcagg tctcgcgcgc gccgaccggg gctcggcccg ggtgatgggc
     1861 catgatgtgc gcaccgacta tcgcgccgcg cgccgttccc tcggcgtcgt ccctcaggaa
     1921 ctggtattcg atccgttctt caccgtgcgc gagaccttgc gcttccaatc gggctatttc
     1981 ggagtgcgca acaacggcgc gtggatcgat gaggtgatgc ggcacctcga cctcgcttcc
     2041 aaagccgaag tcaacatgcg cgcgctctcg ggcgggatga agcggcgggt gctcgtggcg
     2101 caggccctgg tgcacaagcc ccccgtgatc gtgctcgacg aaccgaccgc gggcgtcgac
     2161 gtcgagcttc gccaggcgct gtggcagttc atccagcggc tcaaccggga cggccacacg
     2221 atcgtcctga cgacgcacta cctggaggag gccgaagcgc tttgcgagcg catcgcaatg
     2281 ctgaaacagg ggcgcgtcgt ggcgctcgat tccacctcca acctgctcaa gcgggtacac
     2341 cacctgtttc ttcgcctgcg tttggacggc gagcttcccg gcgagctgcg cccgctcgag
     2401 catggcagca ccgcgggcgc ccacatactg cgcctgaagg gctacgagga cgtcgagcag
     2461 accctcgcgt gcatccgcag ggccggctgc cgcatcgagg aaatggagct gatgcagccc
     2521 gatctcgaag acgccttcgt cgacgtaatg caacgctcat gagcggattc cccacgctgc
     2581 tatataaaga agtgctgcgc ttctggaagg tgagcacgca gacgatcggc gcgccggtgc
     2641 tcaccgcggt ccttttcctg ttcatcttca gccacgtcat ggaaggccgg atccggatgt
     2701 acgacacggt tccgtacgcg gggttcctcg ttccgggcct ggtgatgatg tcggtgctgc
     2761 agaatgcctt tgccaacagc tcatcgtcgc tcgtccagtc caagatgacg ggcaacatca
     2821 tattcgtgct gctgccgccg cttgcgtact gggagttctt tgccgcctac gtactggctg
     2881 ccgccgcgcg ggggatggtc gtgggcgcgg cggtgctgct ggtcacgctg cctttcgtcg
     2941 aaatcgaatt tcgccacccg ctgtggatcg tcgcgtttgc cttctttgcg agcgccgtgc
     3001 tgggctcgct cgggatgatc gcgggaatct gggccgagaa aatcgaccag gtcgccgcct
     3061 ttcagaactt cttcgttatg ccgctcacct ttctttcggg agtgttctac tcgattcatt
     3121 cgctcccccc tttctggcaa gggctatcgc atttcaaccc gtttttctac atgattgacg
     3181 ggttccgcta cggttttttc ggcgtttcgg acgtccaccc gtttcggagc ctgacggtag
     3241 tgacggcttt tctgggcgtg gtttccgcaa tgacgctcgc gatgatcaag agcggttaca
     3301 ggttgaggca ctagcatggt gacgccggag caggttcaga gctacatcga gaaggggctg
     3361 cagtgcagcg cggtgcgcgt cgtgggcgac ggagagcact tcgaggcgct cgtcgtgagc
     3421 gaagcgttcc gcggcaagag ccgcgtgcag cgccatcaac tcgtctatgc ggcgctcggc
     3481 gagcgcatgc gcggggagat ccatgccctg tcgatgcgca cgctgacccc cgaggaagcg
     3541 gccgatggat aagctcatca tccagggagg cgttccgctc tcgggtgaga tccgcgtttc
     3601 gggcgcgaag aatgcggcgc tccccctcat gtgcgccgcg ttgctgacca gcgagcagct
     3661 cacgctttcc aacgtgccgc acctgaggga cgtgagcacc atgctccggc tgctcgcgca
     3721 gatgggggtg gaagtctcgc tcgacgaccg gctgggtctg tcgctgaggg cggaacgtct
     3781 gtccgatccg ctcgctccgt atgacctggt gaagacgatg cgcgcttcga tcctcgttct
     3841 cgggccgctc ctcgcgcgct gcggcgaggc tcgagtgtcg ctccccggtg gatgcgccat
     3901 cgggacgcgc ccggtggacc tgcacgtcaa gggattggcg gcactcggcg ccgaaatctc
     3961 ggtcgagcag ggctacatct ctgcgaaggc gaagcggctg aaagcggccc gcatcctgat
     4021 ggatctcgtg accgtcacgg ggaccgagaa cctgatgatg gccgcctgcc ttgccgaggg
     4081 cacgaccacg ctcgagaatg ccgctcgcga gcccgaagtc gtggaccttg cccaatgcct
     4141 ctcggcgatg ggcgcacgga tcaaaggtgc gggaacggac gtgatcacga tcgaaggcgt
     4201 tgcccggctg cgcgcggccc ggcaccgcat catggccgac cgcattgaaa ccggaacgtt
     4261 ccttgcggcg gctgcagcga ccggcggctg cgtcactctt catggagcga acgccggcat
     4321 cctcgatgcg gtgctcgaaa agcttcgcga agcgggcgcg gaggtgcaaa cgggggacgg
     4381 gaggctctcg ctcacgatgc ggggaaggcc caagggagtc aatgtccgga ccgctcctta
     4441 tccggcgttc ccgactgaca tgcaggcgca actgatggca ctggacgcga tagcggaagg
     4501 caccgcggtt atcaccgaga ctattttcga gaaccgcttc atgcatgccc tggaaatgca
     4561 gcgtctcggc gccgacatcg agatctcggg gaacacggct gtcgtgcacg gcgttgcgaa
     4621 gttgcagggc gcgaccgtca tggcgaccga cttgcgcgcc tcggcgagcc tcgtcatcgc
     4681 aggtctcgtc gccgagggcg agacccgggt cgatcggatc tatcacctcg atcgcggtta
     4741 cgaatgcatc gaggagaagc tcgcgcaact gggcgcgcgc atcaagcgcg ttcattgagc
     4801 cgcggagagt gcccgaagac gctggaaccg ttccgggatt ttcggaacat catgaaaaat
     4861 aaggttttcc ttcgcggctc ccgcgttgtt tgcggtaaaa tctttttttg gccgattcaa
     4921 ccgtgatcac cctcgcgctc tccaaaggcc ggatcttcga tgaaacgctg ccgctgctcg
     4981 ctgcggcggg catcgaaacg ctcgacaatc ccgacacctc gcgcaagctc attctgcgca
     5041 cgagccgcag cgacctgcgg ctgctcatcg tgcgcgctcc ggacgttccc acgtacgttc
     5101 agtacggtgc gtcggaactg gggatcgcag gcaaggacgt gcttctcgag cacggcggcg
     5161 aggggctgta ccagccgctc gacctcggca tcgcatcgtg ccgcatgatg gtcgctgttc
     5221 cggaaggctt cgattacgcg ggcgcggtgc gccagggtgc gcgtctgcgc gtggcgacca
     5281 agtacatgca gaccgcgcgc gagcacttcg cggcgaaagg cgtgcacgtc gatctgatca
     5341 ggctctacgg ttcgatggag ctcgcgccgc tcgtgggtct cgccgacgcg atcgtcgatc
     5401 tggtttccag cggcgatacc ttgcgcgcca atcgcctgca tgtagtcgag gagatccgac
     5461 cgatcagcgc ccgcctcatc gtcaaccagg ccgcgctcaa gctcaagcgc gacgccatcg
     5521 agccgctcct cgaccagttc acgaaggccg cgggaaagca tgcttgagat cgcccgactt
     5581 tcgacgaccc aaagcgattt cgacgcgcgt ctcaaggcgc tcaccgcgtt cgacacgacg
     5641 ttcgaccaga aagtcgagga cgacgtcgca gcgattctcg ctggcgtgag cactaggggc
     5701 gacgcggccg tgcttgagta cacggagcgc ttcgaccgga tcaaggttgc acgggtcgct
     5761 gatctggagc tgccgcgccg agaactcgaa aaggcgcggt cgggattgaa tggaggcgag
     5821 cgcgaggcgc tcgagcaggc cgcggtgcgc atccgcaagt atcacgagcg gcaagctgcg
     5881 aagtcatggc aattcacgga agccgatggc acgacgctcg gccaacgtgt gacgcctctc
     5941 gagcgtgtcg gaatgtacgt ccccggcggt ttggccgcct atccctcgtc ggtgttgatg
     6001 aacgccattc ctgccaggat cgccggggtc cgcgagctca tcatgtgcgt gcccacgcca
     6061 gacggaaaat ccaatccgct cgtgctcgcc gctgccgcga ttgccgaagt cgatagagtg
     6121 ttcaccgtcg gcggggctca ggcgatcgct gcgctggcct acggcacgca gaccatcccg
     6181 caagtcgaca agatcgtggg gcccgggaac gcctgggttg tggcggcaaa gcgccgcgta
     6241 ttcggcacgg tcggtatcga catgatttcc ggcccgaccg agatcctggt gatttgcgac
     6301 ggctcgaccg agccggactg gatcgcgatg gatctttttt cgcaggccga gcaccacgag
     6361 acttcgcagg caatccttgt ttcgcccgac aaggcattcc tcgaacgagt ggacgaaagc
     6421 atcggccgat tgcttgcagg tatgccgcgt gcggaaatca tcgaaaagtc gctcgcacgg
     6481 cgcggcgcgc tgatcaaggt gaaaaacctc gacgaggcgt gtgacatcgc caaccggatt
     6541 gctccggagc acttggagct ctcggtcgcc gaaccgaaaa agtggctgaa gaggatccgc
     6601 cacgccggcg cgatttttct ggggcgttac tcctccgagg cgctgggaga ctactgcgcc
     6661 ggcccgaatc acgtcctgcc gacttcgcgc acggcgaggt tttcctcgcc gttgggggtg
     6721 tacgacttcc agaagcgctc gagcatcatc ggcgtatcgc gcaaaggggc gcagaaactg
     6781 ggcaagctcg ccatgacgct cgcgaacagc gaaggattcc gggcccatgc gcgctccgcc
     6841 gagctccgat tgggaaagac cctcaaggaa aagccgtgag cgccgaaccg gaaaaggtca
     6901 tccgcgacga gattcgggag ctctccgcct atcacgtcca gccggcgcag ggtatggtga
     6961 agctcgacgc gatggagaat ccgtaccggc tgcctgagcc actgcgccgc gagatcggtg
     7021 agctcgcggc aaacgccgag atcaatcgct atccggatcc gactgccccg gccctgatga
     7081 ggcgcctgcg tgaggtcatg ggaatcgggc cggagtacga cgttttgctc ggaaacggtt
     7141 ccgacgaaat cattcacatc atcatgcagt gcgtggcgcg tcccggggcc gtggtgctcg
     7201 cgcctcggcc cgccttcttc atgttcagcc aatatgcgcg gttcgcggga ttgccctact
     7261 tcggcgtgct cctcaacccc gactttaccc tcgataccgg cgcgttcttg cacgccattg
     7321 ccgaacacca gcctgcggtc gtgttcatcg actaccccaa taatccgtcg ggcactctgt
     7381 tttcggacga gggcattgct cgaatcatcg ccgcagcccc gggactggtg gtgatcgacg
     7441 aggcctataa ccccttcgcg cgcaagacgt tcatgaaacg gctggcggaa tttccgaacc
     7501 tcgtggtcat gcgcaccctg tccaagctcg ggctcgccgg catccggctc ggctatgccg
     7561 tcggacggcc cgagtggatc ggcgaattca acaaggtgcg ccccccttac aacgtcaacg
     7621 tgcttaccct gttggtcgcc gagcgcgtcc tggcacacta cgacgtgctc gacgcgcagg
     7681 cgcggagcat caaccaggag cgcgcgcgcc tgggcggcga gctgcgcaag ctgccgaagg
     7741 tgacggtttt tccaagcgac gcgaatttca tcctggttcg cgtacccgac gcccaccggg
     7801 tcttcgaggg catgcggcgc cgcggcgtgc tcatcaagaa cctgcatacc ggtgtgccgc
     7861 tgctcgatca ttgcgtccgg ctcaccatcg ggacgccgga ggagaatcgg cgatgccttg
     7921 ccgcgttcgc ggaagcgctc gccgagggct gatttcgtgt aggctatcgc gctcaagctc
     7981 accgcaatgc ccacgcgcgc cgcaaccgcc gaccgcaaga ccaccgagac gcagatcgcc
     8041 ctttcggtca atgtcgacgg cagcggacgg tggagcattg cgacgggcat tccgtttttc
     8101 gatcacatgc tcgaacaggt tgcgcgccac gcggtgctcg atctcgatat tcgggcaaag
     8161 ggcgacctgc aggtggacgc gcatcatacg gtcgaggaca tcgggatcac cttgggtcag
     8221 gccgtggcgc gcgccgtcgg cgacaagaaa ggcattcggc gttacgggca tgcctacgta
     8281 ccgctcgacg aggcgttgtc gcgcgtcgtg atcgatttct ccgggcgccc cggcctcgag
     8341 taccacgtcc agttcaagcg cgcgctgatc ggcgagttcg acgtcgatct ggtgcacgaa
     8401 ttctttcagg ggttcgccaa ccacgcgcag gtgacgctgc atgtcgacaa cctgcgcggg
     8461 gacaacgcgc atcatcagtg cgaaacgatg ttcaaggcgt tcgcgcgggc tctgcgcatg
     8521 gcgctggaac ccgatccgcg cgccgcgggc accgtgccct ccaccaaggg caccctctag
     8581 ggctctttta cgactgaccc tgcaatgcgc gtcgcggtcg ttgattacgg catgggcaac
     8641 ctccattcgg tctccaaggc ggtggaacat gtagcgccgc acgcgagcgt cgaggtgact
     8701 tccgatcccg cgcacgtggc gggcgccgat cgggtcgtat ttcctggcca gggcgcgatg
     8761 cccgattgca tgcgcgagat ggacgcgcgc ggattacgtc cggcggtgct cgacgccgcc
     8821 cgcagccggc cgtttctggg aatctgcatc ggcttgcaga tgcttttcga gcgcagcgac
     8881 gaaggcgatg tggccgggct cgctctcctg ccgggagcgg tgcgttcctt ccccgcggaa
     8941 gccatggtcg acgatgaggg acgcaagctc aaggttcctc acatcggctg gaaccgcgtg
     9001 aaacaggctg cgccgcacgc cctgtggtcg ggcatcgagg acggcagccg gttctatttc
     9061 gtccacagct actttccgca gacgggggag gccgcgctgg tgcggggtac aacggactac
     9121 ggcgtcccct ttacctgcgc cgtggcacgg gataatattt tcgcggtaca gttccaccca
     9181 gagaaaagcg atactgccgg cctgcggctc ctcgccaact tcctcaactg gaatccctga
     9241 atcctcccat cagccttccc ggttgcccgg ggctttgcgc tgttttccga ctcaatgctg
     9301 atcattccgg cgattgacat caaggacgga cactgcgtcc gcctgaaaca gggaaagatg
     9361 aacgaggcca cggtgttctc ggaagatccc gtcggcatgg ccaagcattg gaaggcgcaa
     9421 ggcgcgcgcc ggctgcacct cgtcgacctg aacggggcgg cggccggcaa acccaagaac
     9481 gagagcgtca tccgcgcgat cctgcaggcg ctcggggatt ccccgccggt gcagctgggc
     9541 gg
//
LOCUS       DASPZD010000483         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_934645, whole genome shotgun sequence.
ACCESSION   DASPZD010000483 DASPZD010000000
VERSION     DASPZD010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934645"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VEM33_09420"
     CDS             complement(<1..717)
                     /locus_tag="VEM33_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYS52088.1"
                     /translation="MAGTLTSRTAAQILVDALKIHGVDTAFCVPGESYLAVLDALYDA
                     REAIRLIVARQDGGAAYMAEAWAKVTGRPGICFVTRGPGATNASIGVHTAQQDSTPMI
                     LFIGQVPGEFSDRESFQEMDYRQMFGPMSKWVAQIERADRVHEYVSRAFHLAMSGRPG
                     PVVLALPEDMLTQTASVPDVAPYQAVQASPSAGDLEKLRGLLAGAKRPLAMLGGTVWT
                     REACRDLQAFAESSGLPIACS"
     gene            776..1249
                     /locus_tag="VEM33_09425"
     CDS             776..1249
                     /locus_tag="VEM33_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYS52089.1"
                     /translation="MVDDIPGLRRILKTSKVIAVVGLSGDWFRPSYFAAKYMLEHGYT
                     VIPVNPGYDAILGQKCYKSLRDIAVKVDIVDCFRKTEEIVPIAEDAIAIGAKVLWQQL
                     GVTNAEAAKKAEAAGLEAVMDRCVKIEHGRLFGGLNWCGVNTRVISAKRPQWLPY"
     gene            1368..>2711
                     /locus_tag="VEM33_09430"
     CDS             1368..>2711
                     /locus_tag="VEM33_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYS52090.1"
                     /translation="MAEPLKILVAEDSETDAELVQRELKRGGLDFQSRRVQTEPDFRR
                     ELDEFQPDLVISDFSMPQFSGPKALAIARESRGDIPFIFISGTIGENVAVDMMKAGAD
                     DYVMKNNLARLVPALKRELREAGVRRGRRLAEEGLRESESRFRQLIEQASDGIFVTDK
                     EGNFILVNSRYCEMLGYSRAELLQLHSEVTYPEGEREKRRQHRAEVRERKTSLYERMI
                     RRRDGSSFPAEVNITLLDNDNVQGIVRDITERKRAQQALVESEAGLRRAQLMAKIAHV
                     VTGPDGSFERWSDTLPQLFGVEPAQMPRSTREWLDLLHPDDREGFRATAIEAARTGMR
                     KDVEYRLRGVDGGWSRVRQVIEPLQGQADAKGGARWFSTLQDVTEHWLAEEKIHRLTR
                     VYAVLSGINALIVRVRDRDELFREACRIGVEAGKFKLAWLGVVDREAARVKPVAWH"
BASE COUNT          514 a          855 c          919 g          423 t
ORIGIN      
        1 tgagcaggcg atcgggaggc ctgagctttc cgcgaacgcc tgcaggtcgc ggcaggcttc
       61 gcgcgtccaa accgtgcctc ccagcatggc gagcggacgt ttcgcgcccg caagcaagcc
      121 tcgcagcttt tccagatcac cggcagacgg actcgcctgc accgcctggt agggtgcgac
      181 gtcgggcacg ctcgcggtct gggtgagcat gtcctcgggc aacgcgagca ccaccggccc
      241 gggccgtcca gacatcgcca ggtgaaaggc gcggctcacg tactcgtgca cgcggtcggc
      301 gcgctcgatc tgcgcgaccc acttcgacat cggcccgaac atctggcgat agtccatctc
      361 ctggaaagac tcgcggtcgg agaactcgcc cggcacctgg ccgatgaaca ggatcatcgg
      421 agtcgagtcc tgctgcgccg tgtgcacgcc gatcgaggcg ttggtcgcgc cgggcccccg
      481 cgtgacgaag cagattccgg ggcgcccggt gaccttggcc caggcctcgg ccatgtaagc
      541 ggcgccgccg tcctggcgcg cgacgatcag gcggatcgcc tcgcgcgcgt cgtagagcgc
      601 gtcgagcacc gcgaggtaac tctcgccggg aacgcagaac gcggtgtcga cgccgtggat
      661 tttcagcgca tcgacgagaa tctgcgcggc ggtcctcgag gtgagcgttc ctgccatcgt
      721 gcggccgttt cccgaaaggc gaaactatac ctcaggattc gatagaatcc cgtccatggt
      781 cgacgacatt cccggcttgc gccgcatcct caagacgagc aaggtcatcg cggtcgtcgg
      841 cctttcgggc gactggttcc ggccgagcta cttcgcggcg aaatacatgc tcgagcacgg
      901 ctacaccgtg atcccggtca atccgggata cgacgccatc ctcggacaga aatgctacaa
      961 gtcgctgcgc gacatcgcgg tgaaggtcga catcgtcgac tgcttcagga aaaccgagga
     1021 gatcgtgccg atcgccgagg acgcgatcgc catcggcgcg aaagtgctgt ggcagcagct
     1081 cggcgtgacc aacgccgaag cggcgaagaa agccgaggcc gcaggactcg aggcggtcat
     1141 ggatcgctgc gtgaaaatcg agcacgggag gctcttcggc gggctcaact ggtgcggggt
     1201 caacacccgg gtgatttcgg ccaagcggcc gcagtggctg ccgtactagg gatttgtccg
     1261 ccctctttac gaattggcat gccagggttt tcctgacacg atgtgtcgag gttcctcgat
     1321 aggcccagcg ctttgacaat ccgggccggg agggtgtact acggcacatg gctgaaccgc
     1381 tcaagatact ggtcgcggag gacagcgaga ccgacgccga gctggtgcag cgcgagctga
     1441 aacgcggagg cctcgatttc cagagccgcc gggtgcagac cgaaccggat ttccgccgcg
     1501 agctcgacga gttccagccc gatctcgtca tctcggattt ctccatgccg cagttcagcg
     1561 ggcccaaggc gctcgcgatc gcgcgcgagt cccggggcga catcccgttc attttcattt
     1621 cgggcacgat cggcgagaac gtcgccgtcg acatgatgaa ggccggcgcg gacgactacg
     1681 tcatgaagaa caatctcgcg cgcctcgtgc ccgcgctcaa gcgcgagctg cgcgaggccg
     1741 gagtgcggcg gggacgcagg ctcgcggaag agggattgcg tgagagcgaa tcccggtttc
     1801 ggcagctcat cgagcaagcc tcggacggaa tcttcgtcac cgacaaggag ggaaatttca
     1861 tcctcgtgaa ctcccggtat tgcgagatgc tgggctacag ccgagctgag cttctgcagc
     1921 ttcacagcga ggtgacctac cccgagggag aaagggagaa acggcggcaa caccgcgcag
     1981 aggtccgcga gagaaaaacg agcctttacg agcgaatgat ccggcgcaga gacggctcca
     2041 gtttcccggc cgaagtcaac atcacacttc tcgacaacga caatgtccag gggatcgtgc
     2101 gcgacatcac cgagcgcaaa agggcacagc aggcgctggt cgagagcgag gccgggctgc
     2161 gccgcgcgca gctcatggcg aagatcgcgc acgtcgtcac ggggccggac gggtcgttcg
     2221 agcgctggtc cgacacgctg ccgcagctgt tcggagtgga gccagctcaa atgccccgga
     2281 gcacgcgcga gtggctggac ctcctgcacc cggacgatcg tgaagggttt cgcgcaacgg
     2341 ccatcgaggc cgcccgcacg ggcatgcgca aggacgtcga ataccggctg cggggcgtcg
     2401 acggcgggtg gagccgggta cgccaagtga tcgagccgct ccagggccaa gccgacgcca
     2461 aaggcggagc gcgatggttc agcacgctgc aggacgtgac cgagcactgg ctcgcggagg
     2521 agaaaatcca ccgcctcacg cgcgtctacg cggtgctgag cggaatcaac gcgctcatcg
     2581 tccgtgtgcg cgaccgggac gagcttttcc gcgaggcgtg ccgcatcggg gtggaggctg
     2641 gaaaattcaa gctcgcctgg ctcggcgtcg tcgatcgcga ggcggcgcgc gtgaagccgg
     2701 tcgcctggca c
//
LOCUS       DASPZD010000484         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1744503, whole genome shotgun sequence.
ACCESSION   DASPZD010000484 DASPZD010000000
VERSION     DASPZD010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744503"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VEM33_09435"
     CDS             <1..861
                     /locus_tag="VEM33_09435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS52091.1"
                     /translation="AAGGSPDLQARIYTQRMSQLYGYVWVIENLPGAGANIAPERVAK
                     APADGYTLLMASAGPLYFNPTLYTKLPYDIQRDFEPITQVSNVPNILAVHPSVPINSV
                     RELIAYARANPGKLRFGSPGSGSSQHLSGELFKKMSGVELQHVPYKSVAQMTTELVSG
                     QFELSFNNAPLVLPHVRSGRLRALAVTTRTRLALAPDVPTLDESGLAGFEVGGGSGVL
                     APKGTPGAILRKLETDLRSVLNSAEVREQYAANGLIAVGTSSAEFAAYMKSEIARWAP
                     LIRATGATVD"
     gene            complement(904..>2839)
                     /locus_tag="VEM33_09440"
     CDS             complement(904..>2839)
                     /locus_tag="VEM33_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52092.1"
                     /translation="WLSDAQLDRCAPAESEPFQSPVPTRMISNGEYMPFPQTQKQKQV
                     ESRVKELAGKASKKLGMTRRQFLEGTGGLAASFLAMNEVYGNTFFKVDEVEMFEKAAF
                     AENATPRNLFVFDDQTHIVRSSTNSPQGLRALAQGPGPVSTAAGFSPATTPPFGNPNN
                     GRGGNPAGVDELGSTWTPWNPAQLHPDFPPNPGPATTVAGEFHLGQYINRMYLEAQTS
                     VSIISNANIALFTPPGGGTPGPATNIHDSLISEILTGWQTAQCRDYINQLAGSTRALA
                     HGQIYPGPGNHADPLFGDYTQWQIENMQPDSWKGYNVAFAASAAPGTAFARWRLDDEA
                     IAYPTYQVIARNKHQLKKHPGFFNICIHKGLSANATQPGGTNNLPNFGNPDDIVKVAT
                     DWPQFNWIMYHSCIRPGFWMLQALQDIENLPGSMQPTLLTDSHGHSVPNIRWSTQFAQ
                     IAGGRYVAGAEPTSASPSSLHRLGNVYAELGTTMASMIVTFPTVWAHLIGQLLHYMGP
                     TNIVFGSDSLWYGGPQWQIEALWRSQIPDDIADRWGYPPLTEGAKRHILGLNSARLYG
                     LPAAAVRYTDGGLPGYATAPELQSGGSMDAALRGVGYPEPVTPASPMPEDRFAKLKRW
                     TDSIGLGRSNTRNGWMRTRV"
BASE COUNT          466 a          912 c          953 g          508 t
ORIGIN      
        1 gcggctggag gatcacccga cctgcaggcg aggatctaca cccagcgcat gtcgcagctc
       61 tacggctacg tctgggtgat cgagaacctg ccgggagccg gggccaacat cgcgcccgag
      121 cgcgtggcga aggcccccgc cgacggctac acgctgctga tggcctccgc ggggccgctg
      181 tatttcaatc cgacgctgta tacgaagctt ccctacgaca tccagcgcga cttcgagccg
      241 atcacgcagg tctcgaacgt gcccaacatc ctcgcggtgc acccgagcgt gccgatcaat
      301 tcggtcaggg agctgatcgc ctacgcccga gcgaacccgg gcaagctgcg cttcggctca
      361 cccggcagcg gcagctccca gcatctctcg ggcgagctgt tcaagaaaat gtccggggtg
      421 gagctgcagc acgttcccta caagtcggtc gcgcagatga ccaccgaatt ggtaagcggg
      481 cagttcgagc tgtcgttcaa caacgcgccc ctcgtcctgc cccacgtcag gagcggaagg
      541 ctgcgcgcgc tcgccgtgac gacgcggacg cggctcgcgc tcgctcccga tgttcccacg
      601 ctcgacgagt caggactcgc cgggttcgag gtcggcggcg gatccggagt gctcgcgccg
      661 aagggcacgc ccggcgcaat cctgaggaag ctcgagaccg acctgcgcag cgtcctcaac
      721 tcggcggagg tgcgcgagca gtacgcggcg aacggcctca tcgccgtcgg aacgagctcc
      781 gcggaattcg ccgcctacat gaaaagcgag atcgcgcgct gggcgccgct gatccgcgcg
      841 accggcgcga ccgtcgattg agcgatcccg ccctcgatct ggtcgatgac gccaggtcgc
      901 ggactacact cgcgttcgca tccagccgtt gcgggtgttg ctccgcccca gaccgatgct
      961 atcggtccat ctcttcaact tcgcaaaacg atcctcgggc atggggctgg cgggcgtcac
     1021 gggttccgga taaccgaccc cgcggagcgc ggcatccatg ctgccgccgg actggagctc
     1081 cggcgcggtg gcatagcccg gcaaaccgcc atcggtgtag cggaccgctg cagcgggaag
     1141 cccgtaaagc cgggcggagt tgaggccgag gatgtgccgc tttgcccctt ccgtcagcgg
     1201 cggatagccc caccggtctg cgatatcgtc ggggatctgc gagcgccaca gcgcctcgat
     1261 ctgccattgc gggccgccgt accagagcga gtccgagccg aagacgatat ttgttggccc
     1321 catgtaatgc aggagctgac cgatgagatg cgcccacacc gtggggaatg tcacgatcat
     1381 cgacgccatc gtcgtcccga gctcggcgta cacgttgccg agacggtgca agctcgaggg
     1441 cgacgcggac gtcggttccg ccccggccac gtacctgccg ccggcgatct gcgcgaactg
     1501 cgtcgaccac cggatgttcg gaacgctgtg cccatggctg tccgtgagca gcgtcggctg
     1561 catggatccc ggcagattct cgatatcctg cagagcctgg agcatccaga agccgggccg
     1621 gatgcaggag tgatacatga tccagttgaa ttgcggccag tccgtcgcta ccttcacgat
     1681 gtcgtcgggg ttcccgaaat tgggaaggtt gttggtgccg ccgggttgcg tggcgttggc
     1741 cgacaggcct ttgtgaatgc agatgttgaa gaagccgggg tgcttcttga gctgatgctt
     1801 gttccgggcg atgacttggt acgtcgggta ggcgatcgcc tcgtcatcga ggcgccagcg
     1861 cgcaaacgcg gtcccggggg ccgcgctggc cgcgaacgca acgttgtagc ccttccacga
     1921 gtccggctgc atgttctcga tctgccactg cgtgtaatcg ccgaagagcg ggtcggcgtg
     1981 atttccgggg ccgggataga tctgcccgtg ggcgagcgcc cgcgtcgagc cggcgagctg
     2041 attgatgtaa tccctgcatt gcgcggtttg ccagccggtc agaatctcgc tgatcagact
     2101 gtcatggatg ttggtcgccg gcccgggcgt gcctccgccg ggcggcgtga aaagcgcgat
     2161 gttcgcgttg ctgatgatgg agacgctcgt ctgcgcttcc agatacatcc gattgatgta
     2221 ctggccgaga tggaattcgc cggccaccgt ggtcgccgga ccgggattcg gaggaaaatc
     2281 gggatggagc tgcgccgggt tccaaggcgt ccacgtgctg ccgagctcat cgacgcccgc
     2341 cgggtttccg ccgcggccgt tgttggggtt gccgaacggc ggcgtcgtgg cggggctgaa
     2401 tcccgcggcc gtggagaccg gacccggtcc ctgcgcaaga gccctcaagc cttgggggct
     2461 gttcgtgctc gagcgcacga tgtgggtctg atcgtcgaag acgaacaggt tgcgtggcgt
     2521 tgcgttctcc gcgaacgccg ccttctcgaa catctcgacc tcgtcgacct tgaagaacgt
     2581 gttgccgtac acctcgttca tcgcgaggaa ggaggcagcc aggccgcccg tgccttcgag
     2641 gaactgccgc cgcgtcatcc cgagcttttt cgaggccttg ccggcgagct ccttgacccg
     2701 ggattcgacc tgcttctgct tctgcgtctg cgggaaaggc atgtactcgc cgttcgagat
     2761 catgcgggtc ggaacggggg actgaaaggg ctcgctctcc gccggagcgc accggtcgag
     2821 ctgcgcgtcg ctcagccaa
//
LOCUS       DASPZD010000485         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_126186, whole genome shotgun sequence.
ACCESSION   DASPZD010000485 DASPZD010000000
VERSION     DASPZD010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126186"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VEM33_09445"
     CDS             <1..592
                     /locus_tag="VEM33_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYS52093.1"
                     /translation="SFGVIEQMRAARPQLRFLELVHRLDRETSGLLILAKKRSALTAL
                     HAALREGRVRKEYLALVKGSWRNDKQRVSLPLKKFLTRSGERRVSVDREGRESQTVFR
                     KKREMRNYTLLSAELLTGRTHQIRVHLNHLGFPIAGDDKYGDFELNRGLAKQGLKRMF
                     LHAGELEFGHPLSGKALKLHAPLPEDLSRFLDSLGC"
     gene            592..1242
                     /locus_tag="VEM33_09450"
     CDS             592..1242
                     /locus_tag="VEM33_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYS52094.1"
                     /translation="MRYPLLVFDWDGTLIDSARTIVACIQAACRDLGLAVPDEARASH
                     VIGLGLKDALSYAIPGLPADDYGRVVEAYRRHFLALDPGIPPFPGAQAMLAGLKARGH
                     ILAIATGKSRAGLERALDNTGLRPLFAASRCADECASKPAPDMLSELMEELGANAVDT
                     LMIGDTVHDLQMAARAAVQAVAVSHGAHPRGDLVALAPLACLANIDELTLWLARNA"
     gene            1221..1583
                     /locus_tag="VEM33_09455"
     CDS             1221..1583
                     /locus_tag="VEM33_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYS52095.1"
                     /translation="MAGSERVICSSADLTDSGDGVRFELEVHGRPEPAFAVRYKGRVY
                     AYLNRCAHMPMELDWKPGKFFDVWGLHLICSTHGATYAPDTGRCLRGPCFQEGLIPVP
                     VEESDGRVIMKGDPRVRR"
     gene            1615..2508
                     /locus_tag="VEM33_09460"
     CDS             1615..2508
                     /locus_tag="VEM33_09460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HYS52096.1"
                     /translation="MAALKEQRRARRWSIFFKLLGFAYLTLLVLLALDWRGHDGLTSG
                     KHTALVDVVGVIDPKGDASADRVTEALQNAFKNKNTQGVILRINSPGGSPVQAGIIYD
                     EIRRLRGIYPNVPMYAVVEDICASGGYYVAAAADKIYVDKASIIGSIGVIMDGWGFTG
                     TMEKLGVERRALTSGENKAFLDPFSPVDEKQKRHAQSMLDEIHKQFIDVVRKGRGKRL
                     HESSDLFSGLLWTGAKSIELGLSDGFGSVDFVAREVIKAEDIFDYTKKQDFTERFAKR
                     FGAAMASALAKVLAQGQFNIR"
     gene            complement(2534..3253)
                     /locus_tag="VEM33_09465"
     CDS             complement(2534..3253)
                     /locus_tag="VEM33_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYS52097.1"
                     /translation="MSSERRGSLYLLPNTLGDGAPEAVIPAGALDRARSLDYIIAEDP
                     KAARAFLKRIATNRPLQSIRIERLDHNTGASDIQAFLEPLLSGSDAGLLSEAGLPAVA
                     DPGASLVRLAHERNIRIVPMSGPSSLVLALAASGLDGQRFAFHGYLPIAEADFVSTLK
                     ECERQSRNLRQTQIFIETPYRNDRTLAAMLRALAPATLVCVATDLTLESETVKTRPVA
                     AWRRETPQLKGRPTVFLLLAE"
     gene            complement(3243..3818)
                     /locus_tag="VEM33_09470"
     CDS             complement(3243..3818)
                     /locus_tag="VEM33_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HYS52098.1"
                     /translation="MQLVLASTSRYRRDLLGRLGIPFSVADPQLAEGARPGETPEGTA
                     RRLAEAKSLAVADRYRDALIIGCDQVAVGNGEVLGKPGTRENAVRQLRTLSGSEAVFY
                     TAVCVHNTSVGTSRTRVVPCRVTFRRLDDGTIDRYLAREQPYDCAGSAKAEGLGIALI
                     AKMEGDDPSALVGLPLIALVDLLQEQGLNVL"
     gene            complement(3852..4277)
                     /locus_tag="VEM33_09475"
     CDS             complement(3852..4277)
                     /locus_tag="VEM33_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HYS52099.1"
                     /translation="MAAAAPAFAQVYKWVDERGVTHYGERPPQGSKASEVPNRLASPA
                     PSGTGAEANNPKDPSPEQSQARPKDQDPRPAVSKGESPQEAEAAKRAQLCNQQRALLE
                     RLKQSPPSYTLNEKGERIPLDNSAAIARQEKFAAEQCRL"
     gene            4410..4784
                     /locus_tag="VEM33_09480"
     CDS             4410..4784
                     /locus_tag="VEM33_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HYS52100.1"
                     /translation="MEQRGVLGLEQLQRLAKMQCSTQGLEYHLRGDRASNGERCLRLS
                     VRGSVQVVCQRCLDPLPVPIAVDAELQLAENLREIAQADDEIDRVLASRRMDVAELVE
                     DEVILALPMAPRHEACVHHPVK"
     gene            4803..4982
                     /gene="rpmF"
                     /locus_tag="VEM33_09485"
     CDS             4803..4982
                     /gene="rpmF"
                     /locus_tag="VEM33_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HYS52101.1"
                     /translation="MAVQQNKKSPSKRGMHRAHDFLINPPIAIEPTTGEVHLRHHISP
                     SGYYRGKKVVKTKSE"
     gene            5066..6097
                     /gene="plsX"
                     /locus_tag="VEM33_09490"
     CDS             5066..6097
                     /gene="plsX"
                     /locus_tag="VEM33_09490"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HYS52102.1"
                     /translation="MEITVAIDCMGGDHGPHVTVPAALNFLKSNSDVDIVLVGLRDAI
                     EAELKARGAKPGPRLRVHHASEVVLMDESPALALRNKKDSSIRVAVDLVKNGEAQACV
                     SAGNTGALMAVSRFVLKMLPGIDRPAIIAALPSVRGTTYVLDLGANVDCGAEHLLQFA
                     IMGATLVAAVEHKDRPSVGLLNIGEEDIKGNEVVKEAAEMLRESGLNFIGNIEGDGIY
                     KGEADVVVCDGFVGNVMLKASEGMVQMVRSFIRQEFMRSVLARLAGVIVMPVLYAFRK
                     RLDPRRFNGATLLGLRGIVVKSHGSADAFGFERAIERAMEEVRNEVLHRLMRRFGQVE
                     RGNSEAVEA"
     gene            6094..>6401
                     /locus_tag="VEM33_09495"
     CDS             6094..>6401
                     /locus_tag="VEM33_09495"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524529.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HYS52103.1"
                     /translation="MIWSRIVGTGSYLPPKVVTNADLAKQMDTSDEWIRTRTGIRQRH
                     IAEPSQTSSDLAVEASRAALAAARIPPQEIDLIIVATSTPDYIFPSTACIMQAKLGIK
                     G"
BASE COUNT         1142 a         1999 c         2133 g         1127 t
ORIGIN      
        1 cagcttcggg gtcatcgagc agatgcgcgc cgcgcggcct cagctcaggt tcctcgagct
       61 ggtgcaccgg ctcgatcgcg agacctccgg gctgctcatc ctcgccaaga aacgctcggc
      121 gctgaccgca ctgcacgccg cgctgcgcga gggcagggtg aggaaggaat atctcgccct
      181 ggtgaaggga agctggcgca acgacaagca gcgcgtcagc ctgccgctga aaaaatttct
      241 cacccgttcc ggagagcggc gcgtcagcgt cgatcgcgag ggcagggagt cgcagacggt
      301 cttccgcaag aagagggaaa tgcgcaacta cacgctgctg tcggcggagc tcctgaccgg
      361 ccgcacccat caaatccgcg tccacctcaa ccacctcgga tttccgatcg cgggtgacga
      421 caaatacggc gacttcgagc tcaaccgggg attggcgaag cagggcctga agcgcatgtt
      481 cctgcacgcg ggcgaactgg agttcgggca tccgctttcc gggaaagccc tgaaactgca
      541 tgcgccgctt cccgaagacc tgtcgcgctt tctcgattcg ctgggatgct gatgcgctat
      601 cccctgctgg tgttcgactg ggacggaacg ctgatcgatt ctgcacgcac cattgtcgcc
      661 tgcattcagg ccgcctgccg cgatctcggg ctggccgtgc ccgacgaggc gcgggcgagc
      721 catgtcatcg gtctgggatt gaaggacgcg ctttcctacg caattccagg cttgcccgcg
      781 gacgactacg gcagggtggt ggaggcgtac cgcagacact ttctcgcgct cgaccccggg
      841 atcccgccct ttccgggagc ccaagcgatg cttgcaggcc tcaaagctcg cggccacatt
      901 ctcgccatcg ccaccggaaa gagccgcgcg ggcctcgagc gggcgctgga taataccggt
      961 ttgcggccct tgttcgccgc ttcgcgctgc gccgacgagt gcgcatcgaa acctgcgccc
     1021 gacatgctca gcgagctcat ggaggaattg ggcgcgaatg cggtcgacac cctcatgatc
     1081 ggagacacgg tgcacgatct tcaaatggct gcacgcgccg cagtgcaggc cgtcgcagtg
     1141 agccacggcg cgcatcccag gggcgacctg gtcgcgctcg cgccccttgc ctgcttggcg
     1201 aacatcgatg agctgacgct atggctggct cggaacgcgt aatttgctcg agcgccgatc
     1261 tgacggattc gggcgacggg gtacgcttcg agctcgaagt gcacggaaga cccgaaccgg
     1321 cgttcgccgt gcgctacaag ggccgggtct atgcctacct caaccgctgc gcgcacatgc
     1381 cgatggagct cgactggaaa cccggaaagt tttttgatgt ctggggcctg catttgatat
     1441 gctcgacgca cggtgcaacc tacgcgccgg atactggccg atgccttcgc ggcccgtgct
     1501 tccaggaggg actcattccg gtccccgtcg aggagagcga cggccgtgta atcatgaagg
     1561 gtgatccgcg tgtccgaagg taactgggaa cgggacgtga tcgagacgct ggcgatggcc
     1621 gccctcaagg agcagcgccg cgcccgccgc tggagcatct ttttcaagct gctcggcttc
     1681 gcctatctca cgctgctggt gctgctcgcg ctcgactggc gtggacacga cggcctcact
     1741 tcgggcaagc acaccgcgct ggtcgatgtg gtgggcgtca tcgaccccaa gggcgacgcg
     1801 agcgccgacc gtgtcaccga agcgctccag aatgcgttca agaacaagaa tacacagggc
     1861 gtgatactgc gcatcaattc gccgggcggc agcccggtgc aggccggaat catctacgac
     1921 gaaatccgcc gcctgcgcgg gatttacccc aacgttccca tgtatgccgt ggtcgaggac
     1981 atctgcgcct cgggcggcta ctacgtcgcg gccgccgccg acaagatcta cgtcgacaag
     2041 gcgagcatca tcgggtcgat cggcgtgatc atggacggct ggggcttcac cgggacgatg
     2101 gagaaactcg gcgtcgagcg gcgcgccctc acctcgggcg agaacaaggc cttcctcgat
     2161 ccgttctcgc cggtcgacga gaagcagaag cgccacgcgc aatcgatgct ggacgagatc
     2221 cacaaacagt tcatcgatgt cgtgaggaag gggaggggaa agcggctcca cgagagctcc
     2281 gatctgttct ccgggctgct gtggacgggc gcgaagagca tcgagctggg actctcggac
     2341 ggctttggca gcgtcgattt cgtcgcccgc gaggtgatca aggccgagga catcttcgat
     2401 tacaccaaga agcaggattt caccgagcgt ttcgccaagc gtttcggcgc ggcgatggcg
     2461 agcgcgctcg ccaaagtgct cgcgcagggc cagttcaaca ttcgttgagc tcgccagccg
     2521 gagcccggcg cgactactcc gcgagcagca ggaataccgt cggccgtccc ttgagttgcg
     2581 gcgtttccct gcgccatgcc gcgacgggtc gcgttttcac cgtttccgat tccagcgtca
     2641 ggtcggtggc gacgcatacg agcgtagccg gggcgagagc acgcagcatc gccgccagag
     2701 tgcgatcgtt gcggtagggc gtctcgatga atatttgagt ctgtctgagg tttcgcgact
     2761 ggcgttcgca ttccttcaag gtggagacga aatcagcctc cgcgatgggg aggtagccgt
     2821 ggaacgcgaa gcgctgcccg tccaggccgg aggctgcgag cgcaagcacg agggacgagg
     2881 ggccgctcat cggcacgatg cgtatgttgc gctcgtgcgc gaggcgcacg aggcttgcgc
     2941 cggggtcggc aacggccggc aagcccgctt cggaaaggag tcccgcgtca ctgccgctga
     3001 ggagcggctc gaggaaggcc tgaatatcgc tcgccccggt gttgtggtcg aggcgttcga
     3061 tgcggatgct ctgcaacggc cggttcgtcg cgatgcgttt caggaaagcg cgcgccgctt
     3121 tggggtcctc ggcgatgata tagtcgagcg accgcgcgcg atcgagcgcg ccggccggga
     3181 tgaccgcctc cggcgcaccg tcacccagcg tgttgggcag cagataaagc gagccccttc
     3241 gctcagagga cattcaaccc ttgctcttgc agcagatcga ccagcgcgat cagcggcaag
     3301 ccgacgagcg cgctcgggtc atcgccttcc attttcgcga tcagcgcgat tcccaggccc
     3361 tcggccttcg cgctgccggc gcagtcgtag ggttgctccc gggcgagata gcggtcgatc
     3421 gttccgtcgt cgagcctgcg gaaggtgacg cggcagggga ccacgcgggt gcggctcgtc
     3481 ccgaccgagg tgttgtgcac gcacactgcc gtgtaaaaca ccgcctcgct gcccgacagg
     3541 gtgcgcagct ggcgcaccgc gttctcgcga gtgcccggct tgccgagcac ctcgccgttt
     3601 cccacggcga cctgatcgca gccgatgatc agggcgtcgc gatagcggtc tgcgaccgcg
     3661 agcgacttgg cctccgcgag gcgccgcgcc gtgccctccg gggtttcgcc ggggcgggct
     3721 ccctctgcga gctgcggatc ggcgacggag aatggaatcc cgagccgacc gagcaggtcg
     3781 cgccgatagc gcgaggttga agcgagaacg agctgcatgt ttcgggccct cgagtcacgc
     3841 ttgaaacggc gctagagtct gcactgctcg gcggcgaact tttcctgccg cgcgatggcg
     3901 gctgaattgt ccaagggtat ccgctcgccc ttttcgttca gcgtgtagct cggcgggctt
     3961 tgcttcaaac gttccagcag ggcgcgttgc tggttgcaga gttgggctcg cttggccgct
     4021 tccgcctcct gcgggctttc acccttgctg acggccggac ggggatcctg atccttgggc
     4081 cgtgcctggc tctgctcggg gctcggatcc tttgggttgt tcgcctcggc gccggtaccg
     4141 ctcggggcgg gcgacgcaag cctgttcggg acttcgcttg ccttgctacc ctggggaggt
     4201 cgttcgccat agtgcgtcac gccgcgctcg tctacccact tgtagacctg ggcgaacgcg
     4261 ggcgcggccg cggccacgaa gaacaaaaag tgcagtaacg ctctgaacaa cttggaattt
     4321 cttttgacac tgaagaacaa acataatatc atgcgcccct tatgtcgcaa gcgggggtga
     4381 tagacagcct ccattttgcc cgcgccgcat tggaacagcg gggagtgctg ggcctggagc
     4441 aactgcagcg cctggcgaag atgcagtgct cgacccaggg gctggagtat cacctgcgcg
     4501 gagatagggc gagcaacggc gagcggtgtt tgcggttgtc ggtaagaggg tcggtgcagg
     4561 tggtctgtca gcgttgcctc gatccgctcc cggttcccat cgcggtcgac gccgagttgc
     4621 agctcgccga gaacctgcgc gagatcgcgc aagccgacga cgagatcgac cgcgtgctcg
     4681 catcccgccg catggacgta gcggagcttg tcgaggacga ggttatcctg gctttgccga
     4741 tggcgccgcg gcatgaagcg tgcgtgcacc atccggtgaa atgagtttga aggagctccg
     4801 atatggcagt ccagcagaac aagaaatcgc cgtcgaagcg cggcatgcac cgcgcccatg
     4861 atttcctcat caatccgccg atcgcgatcg agccaacgac gggcgaagtg cacttgcgcc
     4921 atcacatcag ccccagcggt tactatcgcg gcaagaaggt cgtcaagacc aagagcgagt
     4981 agacttcttc tggccgcagt gaccggacag agtcgggatc gaccctggca ttgagcgaag
     5041 caggaaccgc ttcgcaccga gcctcatgga aatcacagta gccatcgatt gcatgggcgg
     5101 cgatcatggt ccgcacgtca ccgtgccggc ggcgctcaat ttcctgaaat ccaactcgga
     5161 cgtcgacatc gtcctggtgg gcttgcgcga cgccatcgag gcggagctga aggcgcgcgg
     5221 cgccaaaccc ggtccgcgcc ttcgggttca ccacgcgagc gaggtcgtgc tgatggacga
     5281 atctccagct ctcgcgctgc gcaacaagaa ggattcgtcg attcgcgtcg cggtagacct
     5341 cgtcaagaac ggcgaagcgc aggcctgtgt cagcgccggt aacacggggg cgctgatggc
     5401 ggtgtcgcgc ttcgttctca agatgcttcc gggcatcgac cgccccgcga tcatcgccgc
     5461 gctgcccagc gtgcgcggca ccacctacgt ccttgatctc ggcgccaacg tcgattgcgg
     5521 ggcggagcat ctgctgcagt tcgcgatcat gggggcgacg ctggtcgctg cggtggagca
     5581 caaggaccgg ccgagcgtcg gcctgctcaa catcggcgag gaggacatca aaggcaacga
     5641 ggtggtgaag gaggcggcgg agatgttgcg cgaaagcggg ctcaatttca tcggcaacat
     5701 cgaaggcgac ggaatctaca agggggaagc cgacgtggta gtgtgcgacg gcttcgtcgg
     5761 caacgtcatg ctcaaggcgt ccgagggaat ggtgcagatg gtgcgcagct tcatccgcca
     5821 ggagttcatg cgcagcgtcc tcgccaggct cgccggcgtg atcgtcatgc cggtgctcta
     5881 cgcgttccgc aagcggctcg atccgcgccg cttcaacggc gcgaccttgc tcggattgag
     5941 aggcatcgtc gtgaagagtc acgggtctgc cgacgcgttc gggttcgagc gcgccatcga
     6001 gcgggcgatg gaggaagtgc gcaacgaagt gctgcaccgc ctcatgcggc ggttcggcca
     6061 ggtcgagcgg gggaattcgg aggcggtgga agcgtgatct ggtcgcgcat cgtcggtacc
     6121 gggagctatt tgccgccgaa ggtcgtcacc aacgccgatc tggccaagca aatggacacc
     6181 tccgacgaat ggatccggac gcgcaccggc atccgccagc gccacatcgc cgaaccctcg
     6241 cagacctcga gcgatcttgc cgtcgaggcg agccgcgctg cgcttgccgc ggcgcgaatc
     6301 ccgccgcagg aaatcgatct catcatcgtg gccacgtcga ccccggacta catctttccg
     6361 agcacggctt gcatcatgca ggcgaagctg ggaataaaag g
//
LOCUS       DASPZD010000486         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_629103, whole genome shotgun sequence.
ACCESSION   DASPZD010000486 DASPZD010000000
VERSION     DASPZD010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629103"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEM33_09500"
     CDS             <1..207
                     /locus_tag="VEM33_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HYS52104.1"
                     /translation="TRRERIAAPKSIEVEVDSPKVTLRGSNEASVTFRQNYRSDKLTS
                     SNTKTLELVKADGRWRIREEKAGK"
     gene            204..1364
                     /locus_tag="VEM33_09505"
     CDS             204..1364
                     /locus_tag="VEM33_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYS52105.1"
                     /translation="MRSVLLLLGALGFAVAAAAATPEDRLSAVFEAIEANRVDEAMRR
                     VDALLRDYPNFRLAYLVRGDLLLARSRPLLTFGNVVKTVPQDRIEGLREEALARLRAM
                     RSRPSEDRLPRYVLQLHPGQKHVLVVDSRRSRLYVFGNDQGRPRLVADFYVTLGKNGV
                     EKTREGDQKTPIGVYHVTGNLPRSKLTDFYGAGAFPINYPNEWDRRRGRKGHGIWLHG
                     VPAELYSRPPRASDGCLVLANPDLESIGRYVQVGTTPVIIADAIEWSDAASVDAERRS
                     LNAALEQWRADWQSRDTERYLGHYSQRFGAGGDQGYAAWAAHKRAVNAAKGWIKVELS
                     RVAMFRYPREGDFVVVSFEQDYKSSTLSNTMRKRQYWIKEEGRWKILYEGAG"
     gene            1361..1945
                     /locus_tag="VEM33_09510"
     CDS             1361..1945
                     /locus_tag="VEM33_09510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYS52106.1"
                     /translation="MKLKYLFALSTAAVVPAALAAEPQVDVRTNVGTIRLELYPEKAP
                     KTVENFLQYVRDGHYNGTVFHRVIDGFMIQGGGFDGGFKQKKTRSPIANEAQAGVKGG
                     LKNEPGSVAMARTADPNSATAQFFINVGDNNFLNWGDPRSDGNGYAVFGKVVSGMDVV
                     TKIAKTPTGPGGPFPRDVPRSPVVIESMTVVSGK"
     gene            1945..>2457
                     /locus_tag="VEM33_09515"
     CDS             1945..>2457
                     /locus_tag="VEM33_09515"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HYS52107.1"
                     /translation="MRALFASDLHLSEDRPEANERLIAFLEGKARSADALYLLGDLFE
                     YWIGDDGLDEAFNAVIAGLLRDLTRRGARLCVMHGKRDFLIGERFCRETGAQLLADPT
                     LEEINGVKTLLMHGDTLCTDDVDYQSWRATARSSAWQQQFLAQPVAERRSAILALREK
                     SKAVIQAKPAA"
BASE COUNT          443 a          819 c          831 g          364 t
ORIGIN      
        1 acgcggcgcg agcgcatcgc cgcacccaag tcgatcgagg tcgaggtcga ctcgcccaaa
       61 gtgaccctgc gcggcagcaa cgaggcgagc gtcacgttcc gccagaatta ccgctctgac
      121 aaactcacga gctcgaacac caagaccctg gagctggtga aggccgatgg ccgctggcgg
      181 atccgggaag agaaggcggg caaatgagga gcgttctgct tctcctgggg gcgctgggct
      241 ttgccgtcgc ggcagcggcc gcgacccctg aggatcgcct gtcggcggtg ttcgaggcga
      301 tcgaggcgaa ccgcgtcgac gaggcgatga ggcgcgtcga cgcgctgctc cgcgactatc
      361 ccaacttccg cctcgcgtac ctggtgcgcg gcgacctgct gctggcgcgc tcgcggccgc
      421 tgctgacctt cggcaacgtc gtcaagacgg tgccgcagga ccgcatcgaa ggcctgcgcg
      481 aagaggcgct ggcgcgcctg cgggcgatgc gctcgcgccc ctccgaggac cggctgccgc
      541 gctacgtgct gcagcttcat cccgggcaga agcacgtcct ggtggtcgac tcgcgccgct
      601 cgcggctcta cgtgtttggc aacgaccagg gccggccgcg gctggtggcg gacttctacg
      661 tcacgcttgg caagaacggc gtcgagaaaa cccgcgaggg cgaccagaag acgcccatcg
      721 gcgtctacca tgtcaccgga aacctgccgc gcagcaagct caccgacttc tacggcgcgg
      781 gcgcgttccc catcaactac cccaacgaat gggaccgccg gcggggacgc aaagggcacg
      841 gcatctggct gcacggcgtg ccggccgagc tctacagccg cccgccgcgc gcgagcgacg
      901 gctgcctggt cctcgccaac cccgacctcg aatcgatcgg tcgctacgtg caggtcggga
      961 cgacaccggt catcatcgcc gatgcaatcg agtggagcga tgccgcatcg gtggacgccg
     1021 agcgccgctc gctcaacgct gcgctcgagc agtggcgcgc ggactggcag agccgcgaca
     1081 ccgagcgcta cctcgggcat tattcgcagc gcttcggcgc aggcggcgac cagggctacg
     1141 ccgcctgggc cgcgcacaag cgcgcggtga acgctgccaa gggctggatc aaggtcgagc
     1201 tgtcgcgcgt ggcgatgttc cgctatccgc gcgagggcga cttcgtggtg gtgagcttcg
     1261 agcaggatta caaatcgagc acgctgtcga acaccatgcg aaagcgccag tactggatca
     1321 aggaggaagg gcgctggaaa attctgtacg aaggagcagg atgaaactca aatatctctt
     1381 cgcgctgtcg accgccgcgg tggtcccggc cgccctcgcc gccgagccgc aggtggacgt
     1441 gcgcacgaac gtcggcacga tccggctcga gctgtacccg gagaaagcgc cgaaaaccgt
     1501 ggaaaatttt ctgcaatacg tgcgcgacgg ccactacaac ggcaccgtgt tccatcgcgt
     1561 gatcgacggc ttcatgatcc agggcggcgg cttcgatggc ggcttcaagc agaagaagac
     1621 gcgctcgccc atcgccaacg aggcgcaggc cggcgtgaag ggcgggctga agaacgagcc
     1681 cggcagcgtg gccatggcgc gcaccgcgga tcccaactcg gcgacggcgc agttcttcat
     1741 caacgtcggc gacaacaact tcctcaactg gggcgatccg cgcagcgacg gcaatggcta
     1801 cgccgtgttc ggcaaggtgg tttcagggat ggacgtggtc accaagatcg cgaagacgcc
     1861 gaccggcccc ggcggcccct tcccgcgcga tgtgccgcgc tcgccggtcg tcatcgagtc
     1921 gatgacggtg gtgagcggga agtagtgcgc gcgcttttcg cctccgatct ccacctcagc
     1981 gaggatcgtc ccgaagcgaa cgagcgcctg atcgccttcc tcgaaggcaa agcgcgctcc
     2041 gccgacgcgc tgtacctcct cggcgacctg ttcgagtact ggatcggcga tgacggcctt
     2101 gacgaggcct tcaatgccgt catcgccggt ctccttcgcg acctgacccg gcgcggcgcg
     2161 cggctgtgcg tcatgcacgg caaacgcgac ttcctgatcg gcgagcggtt ctgcagggaa
     2221 accggggcgc agcttctggc ggatccgacc ctcgaagaga tcaacggagt caagactctt
     2281 ctgatgcacg gggataccct gtgcacggac gacgtcgact accagtcatg gcgcgcgacg
     2341 gcgcgctcga gcgcctggca gcagcagttc ctggcgcagc cggtcgccga gcggcgcagc
     2401 gcgatcctgg cattgcgcga gaaaagcaag gcggtcatcc aggcgaagcc cgccgcg
//
LOCUS       DASPZD010000487         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1268871, whole genome shotgun sequence.
ACCESSION   DASPZD010000487 DASPZD010000000
VERSION     DASPZD010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268871"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..743
                     /gene="tmk"
                     /locus_tag="VEM33_09520"
     CDS             135..743
                     /gene="tmk"
                     /locus_tag="VEM33_09520"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYS52108.1"
                     /translation="MSRGKFITLEGIDGAGKSTHVGGIADFLRGRGKDIVVTREPGGT
                     PLGEKMRALVLSQTMDIDTEALLMFAARREHIAQVIAPALASGKWVVSERFTDATYAY
                     QGAGRGMASDRIAALERWVHGGLQPDLTLVFDAPVEVALARLAKKRGDRFELESKAFF
                     ERVRTAYLERAAAEPRRMRVVQSARSLPEVRRDVEDIVSTLC"
     gene            746..1738
                     /gene="holB"
                     /locus_tag="VEM33_09525"
     CDS             746..1738
                     /gene="holB"
                     /locus_tag="VEM33_09525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HYS52109.1"
                     /translation="MIFEWQQRPWKDWTRLRERLPHAILMQSGEGLGEFEFAQACAQS
                     LLCEGPRPDERPCGACQACGWFSLGNHPDFRLIVPESMAPEPREEGAEPAKKKSEQIR
                     IEQVRELADFLAVGTHRGGLRVILVYPAEAMNANTQNALLKNLEEPPPATVFLLVTTQ
                     PERLLATVRSRCLRFALPFPPPEPVLRWLEEQGLKQPEATLAGAGGAPLAALKAADTD
                     AERLLFIGSLGSPGFDPIALAETVLRVPLWDLVGWLQRWTYDLLLARVAGRVRYSPDH
                     ERVISDTACHCQAADIAAYLRRLAQARALARHPLNAKLFVEDLLLQYQRLVARP"
     gene            1735..2562
                     /locus_tag="VEM33_09530"
     CDS             1735..2562
                     /locus_tag="VEM33_09530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYS52110.1"
                     /translation="MNGKGDPASPATADFPGWVDSHCHLDFPEFDGKMDELRTEMRAN
                     GVTHALCISVNLAGFPKVLALAEAYDNFFATVGVHPDHEDRTEVDPARLVELARHPRI
                     VAIGETGLDYYRLSGDLAWQRERFRAHIRAARECGKPLVIHTRQAAEDTLTVMREEGA
                     AEVGGVMHCFTESRAMAEAVIALGFHISFSGIVTFKNAGALKDVARWVPLERLLVETD
                     SPYLAPVPHRGKTNRPGLVRCVAEEVARLRGISFEELARATTGNFFSLFDTAKKGRT"
     gene            complement(2627..>2999)
                     /locus_tag="VEM33_09535"
     CDS             complement(2627..>2999)
                     /locus_tag="VEM33_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYS52111.1"
                     /translation="QRNPEDIGAWNLLGESQRQAGQSGQAVRTLERASVIGRTSFATF
                     FLLGEAYRDSNRLDRAVTAYREAARLEPDFARAWFELGAAHVRLGERDEVPEALERLE
                     KLDPTLAVELKKRLEAKPQRR"
BASE COUNT          516 a          952 c         1007 g          524 t
ORIGIN      
        1 catcccgccc ggaccgacgc cctgtatttc gtgtcccgcg gggacgggac gagcgaattc
       61 tcgcgaacgc tcgtcgagca caaccgcgcg gtcgccaaat accaactgcc cggcgcgcgc
      121 cggcgttcgg gccgatgagc cggggaaagt tcatcaccct ggagggcatc gacggtgcgg
      181 gcaagagcac gcacgtcggc gggatagcgg atttcctgcg cggccgcgga aaggacatcg
      241 tggtcacacg cgagccgggc ggtacgccct tgggcgagaa aatgcgtgcg ctcgtgctct
      301 cgcagacgat ggacatcgac accgaggccc ttttgatgtt tgccgcgcgc cgcgagcaca
      361 tcgcgcaggt gattgcgccc gcgctcgcct cgggaaagtg ggtcgtttcc gagcgcttta
      421 ccgacgccac ctatgcgtat caaggcgcag gccgtggaat ggcgagcgat cggatcgcgg
      481 cgctcgagcg ctgggttcac ggcgggctgc agcccgacct gactctcgtt ttcgacgcgc
      541 cggtcgaggt cgcgctcgcg cgacttgcga agaagcgagg cgaccgcttc gagctcgaga
      601 gcaaggcctt tttcgaacgc gtccggaccg cgtatctcga gcgcgcggcg gccgaacccc
      661 gccggatgag agtagtccag tcggcgaggt ccctgcccga agtcaggagg gacgtcgagg
      721 atatcgtttc gacgctgtgc tgaaaatgat atttgagtgg cagcagcgac cgtggaagga
      781 ttggacccgc ctgcgggagc gcttgcccca cgcgatcctg atgcagagcg gggagggcct
      841 gggtgaattc gaattcgccc aggcgtgcgc gcaatcgctg ctgtgcgaag gtccccggcc
      901 ggacgagcgg ccatgcggcg cctgccaggc ctgcggctgg ttctcgctcg gcaatcaccc
      961 cgacttccgg ctgatcgtac ccgagagcat ggcgcccgaa ccccgcgagg agggcgcgga
     1021 gcccgcgaag aagaagagcg agcagatccg catcgagcag gtgcgcgagc tcgccgactt
     1081 cctcgcggtc ggaacgcatc gggggggtct gcgcgtcatc ctggtctatc ccgcggaagc
     1141 gatgaacgcc aatacgcaga acgcgttgct gaaaaacctg gaggaacctc cgcctgccac
     1201 ggtttttctt ctcgtcacta cgcagcccga gcgcttgctc gcgacggtgc gaagccgctg
     1261 cctcaggttt gcgctgccct tcccgccgcc agagccggtc cttcgctggc tcgaggagca
     1321 gggcctcaaa cagccggagg cgacgctcgc cggcgccgga ggtgcgccgc ttgccgcgct
     1381 caaggccgcc gacacggacg ccgaacgcct gctcttcatc gggagccttg gaagtccggg
     1441 cttcgacccg atcgcgctcg ccgaaacggt tctgcgcgtt cccctgtggg acctggttgg
     1501 ttggctgcag cgatggacct atgacctgtt gctcgcccga gtcgcaggtc gggtacggta
     1561 tagcccggac catgaacggg tgatttcgga tacggcgtgt cactgccagg ctgcggatat
     1621 cgcggcttat ctgcgccggc tggcgcaagc ccgggcgctc gcgcggcacc cgctcaacgc
     1681 caagctgttt gtcgaggacc tcctgctgca atatcagcgg ctggtcgccc ggccgtgaac
     1741 gggaaaggag acccggcgtc gcccgcgact gcagactttc ccgggtgggt cgattcgcac
     1801 tgtcatctgg attttccgga attcgacggc aagatggacg aattgcggac ggagatgcgc
     1861 gccaacggag tgacgcacgc gctgtgcatc agcgtcaatc tcgccgggtt tcccaaggtg
     1921 ctggcgctcg ccgaggctta cgataatttc ttcgcgaccg tcggcgtgca tccggaccat
     1981 gaggatcgca ctgaggtgga tccggcgcgg ttggtcgagc tcgcacgcca cccgcggatc
     2041 gttgcgatcg gcgagaccgg gctcgattac taccgcctga gcggtgatct cgcgtggcag
     2101 cgcgagcgct ttcgagccca catacgcgcc gcgcgcgaat gcggcaagcc gctcgtcatc
     2161 catacgcgcc aagctgccga ggacacgctg accgtcatgc gggaagaggg cgcggccgaa
     2221 gtaggggggg tgatgcactg tttcaccgag tctcgggcga tggccgaggc ggtgatcgct
     2281 ctggggttcc atatctcgtt ctcgggaatc gtgaccttca agaacgccgg agccttgaaa
     2341 gacgtggccc ggtgggtgcc gctcgagcgc ttgctggtcg aaaccgactc accttacctc
     2401 gctccggttc cccatcgggg aaaaaccaat cggcccggtc tagtccgatg cgtcgcagag
     2461 gaagtggcgc gcctccgtgg catctcgttc gaggagctgg ccagagcgac cacgggtaac
     2521 ttcttcagtc tcttcgatac agcgaagaag gggaggacct gaggtttcgt ttgagactga
     2581 gactgaattt gacataatat acatatgcga caatacgcct caggggtcag cggcgctgcg
     2641 gcttggcttc cagccgcttc ttgagctcta ccgccagcgt cggatcgagc ttctccagcc
     2701 gctccaaggc ctcggggact tcgtcgcgct ctcccagccg aacatgtgcg gcgcccagtt
     2761 cgaaccaggc gcgggcgaaa tccggttcca ggcgtgcggc ctcccgatac gcagtgacgg
     2821 cacggtcgag ccgattggaa tcgcgatagg cctctccaag aaggaagaag gtcgcaaagg
     2881 aggttcggcc tatgacgctc gcgcgctcca gggtgcgaac cgcttgaccc gactgccctg
     2941 cctgacgctg ggactcccca agcaggttcc aggccccgat gtcctccggg ttgcgttga
//
LOCUS       DASPZD010000488         6543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_399495, whole genome shotgun sequence.
ACCESSION   DASPZD010000488 DASPZD010000000
VERSION     DASPZD010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399495"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2286)
                     /locus_tag="VEM33_09540"
     CDS             complement(<1..2286)
                     /locus_tag="VEM33_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HYS52112.1"
                     /translation="MDEILRRSALEYHLRQPPGKISVTPTKGLLNQRDLALAYTPGVA
                     AACEEIVRDPAEARKLTSRGNLIGVVTNGTAVLGLGAIGPLASKPVMEGKSVLFKKFA
                     GIDCFDIEVNERDPDKLVEIIAALEPTFGGINLEDIKAPECFHIEKKLRERMKIPVFH
                     DDQHGTAIIVGAAVLNGLRVVGKDMAKAKLVCSGAGAAALACLDLLVSLGLKPDNIWV
                     SDIKGVVYQGRKEEMDPNKARYARRTSARTLAEIMPGVDVFLGLSAGGVLKAEMVAQM
                     ADKPLILALANPEPEILPDVAKSMRPDAVMATGRSDFPNQVNNVLCFPFIFRGALDVG
                     ATAINEPMKLAAVRAIADLAMAEQSEIVAAAYGEQALKFGPEYLIPKPFDPRLIVKIA
                     PAVAKAAMDSGVATRPIADFDAYNNQLLQFVYHSGLIMKPIFNAAKEGPRRRIVFAEG
                     EDERNLRAAQVVVDEGLARLILVGRPSVVERRIERHGLRIRPGTDFDLVNPESDPRYR
                     DYWTEYHRLTERRGVSLQFAQVEMRRRHALIGAMMIHRGEAEGMICGTFGTYGLHLRY
                     IDQVIGLRPGAKNFYAMNALMLPKRTVFICDTYVNLDPTAEQLAEMTALAAEVIRRFG
                     VVPRAALLSHSSFGTSDAPSAQKMRAALELIRSLAPELEIEGEMHGDAALDADLRLAN
                     FPRSRLKEEANLLIMPTLDAANIAFNLLKTAAGDGITIGPILLGAARPVHILTSSATV
                     RRIVNMTALTVVDANALDDEKQ"
     gene            2418..3611
                     /locus_tag="VEM33_09545"
     CDS             2418..3611
                     /locus_tag="VEM33_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYS52113.1"
                     /translation="MPNANPVLAARMADIEAFHVMDVQNRAHELEAAGRRIVHMEIGQ
                     PDFAAPPLVAEAAIEAIRQRRLGYTASIGIPELRQAISDHYRERLGVTVPSSRIVVTA
                     GASGAFLLTLGALVNPGDEVLMPDPCYPCNRHFVRLLEGRPRAVPVEENQHYQLADAD
                     IRSHWSRATRGVLLASPSNPTGTMIPREELRAIIASVRRAGGFVVMDEIYQGLVYDGE
                     ASTVLELADDVFVVNSFSKYFNMTGWRLGWIVAPEAYVREIEKLAQNAFICPSAPAQY
                     AALAAFRPETLAVLEERRREFRRRRDYMVPALRALGFRIPLMPQGAFYIYAGCERFSE
                     DSTQFALRVLEEAGVAITPGLDFGSNRPERHVRFAYTRSLEDLKEGVERIARMLGGGD
                     APRSY"
     gene            complement(3603..4415)
                     /locus_tag="VEM33_09550"
     CDS             complement(3603..4415)
                     /locus_tag="VEM33_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYS52114.1"
                     /translation="MQQTLAIIEKQTEASKGFKGGVGYQELYLLFGSKRMEVGAMNTT
                     WTRQGLLDQFGRVATILHHGLAFIGLAAVVIILVRGKTLFFDEDASRSAAVGSIRYDG
                     AVSLFEGADDADTQKYRALANYVSRRYRIAPDVSEQFVGAAYDAGRQVGLDPLLILAV
                     MAVESRFNPVAESLVGARGLMQVIPKYHLDKLLEHGGEEVVLDPMINIALGARILKEY
                     VRRTGSLEAGLQFYNGALADPSSQYAQKVFAEKERLQQAVRQFGRPGARSSV"
     gene            4672..4902
                     /locus_tag="VEM33_09555"
     CDS             4672..4902
                     /locus_tag="VEM33_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52115.1"
                     /translation="MAADPAIVAAGVAAFLAALPLGLAFFRADFFLAAFFAGFFSDFL
                     FPFFRAAFLFFFFFAAFLPFLFFAMAGLHQEL"
     gene            complement(5009..6088)
                     /locus_tag="VEM33_09560"
     CDS             complement(5009..6088)
                     /locus_tag="VEM33_09560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HYS52116.1"
                     /translation="MPLESVPTAVPTERYSAGNSQRRVRVEDKAIINARADVNQLVPF
                     KYKWAWEKYLAACANHWMPQEVNMSRDIALWKDANGLTEDERRLVKRNLGFFVTADSL
                     AANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLRLDEGEVFNAYHEIP
                     SIRDKDEFLIPFIDTLTDPTFRTGTPEADQKLLRSLIVFACVMEGMFFYVGFTQILAL
                     GRQNKMTGSAEQYQYILRDESMHCNFGIDLINQIKMENPHLWTPEFREEIQSLIKKAV
                     ELEYRYAEDTMPRGVLGLNAPMFKEYLRYIANRRCQQIGVDAMFSGASNPFPWMSEMT
                     DLKKERNFFETRVIEYQTGGALSWD"
     gene            complement(6191..6543)
                     /locus_tag="VEM33_09565"
                     /pseudo
     CDS             complement(6191..6543)
                     /locus_tag="VEM33_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448001.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     frameshifted; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
BASE COUNT         1148 a         2077 c         1975 g         1343 t
ORIGIN      
        1 ctgcttttcg tcgtccaagg cgttggcgtc caccaccgtc agggcggtca tgttgacgat
       61 gcgcctgacc gtcgccgaag aggtgaggat atgcacgggc cgcgctgcgc cgagcaggat
      121 cggcccaatc gtgatgccgt cgcccgcggc ggtcttcagc agattgaaag cgatgttggc
      181 ggcgtccagc gtcggcatga tcagaagatt cgcctcctct ttcaggcgcg agcgcggaaa
      241 attcgcaagg cgcaggtcgg cgtcgagggc ggcgtcgccg tgcatctcgc cttctatttc
      301 gagctccgga gcgagcgaac ggatcagctc cagcgccgcg cgcatcttct gcgcggaagg
      361 cgcatcgctc gtgccgaaac tcgagtgcga gagcagggcc gccctgggga ccacgccgaa
      421 gcggcggatc acctccgcgg caagcgcggt catctccgcg agctgctccg cggtagggtc
      481 gagattgacg tatgtgtcgc agatgaagac cgtgcgcttg ggcagcatga gcgcattcat
      541 cgcatagaaa ttcttcgcgc caggccgcaa gccgatgacc tggtcgatgt accgcaggtg
      601 caatccatac gtcccaaagg tgccgcagat catcccctcg gcctcgccgc ggtgaatcat
      661 catcgcgccg atcagcgcgt ggcggcgccg catttccacc tgcgcgaatt gcagcgagac
      721 tccgcgtctc tcggtgaggc ggtggtattc cgtccagtaa tcgcgatagc gagggtccga
      781 ttcggggttc acgaggtcga aatccgtccc tggacggata cgcaatccat ggcgttcgat
      841 gcgccgctcg acgacggagg gccgtcccac caggatcagc ctcgcaaggc cctcgtccac
      901 caccacctgc gccgcacgca ggttacgctc gtcctctccc tcggcaaaca cgatccgacg
      961 ccgcggacct tccttcgccg cgttgaagat cggcttcatg atcagcccgg agtgatagac
     1021 gaattgaagg agctggttgt tgtatgcgtc gaaatcggcg atcggacgcg tggcgacgcc
     1081 ggagtccatg gcggccttcg ctaccgcggg ggcgatcttt acgatcaggc gcgggtcgaa
     1141 gggcttggga atcaggtact cgggcccgaa cttcagtgct tgctcgccgt aggcggcagc
     1201 gacgatctcc gactgctcgg ccatcgcaag atcggcaatg gcacggaccg cggcgagctt
     1261 catcggctcg ttgatcgcgg tcgccccgac gtcgagcgca ccgcgaaaga tgaacggaaa
     1321 gcacaggacg ttgttcactt gattcgggaa atccgaacgc cccgtcgcca tgactgcgtc
     1381 ggggcgcatg ctcttcgcga catcagggag aatctcaggt tcggggttgg cgagcgcgag
     1441 gatcagaggc ttgtcggcca tttgcgccac catctccgcc ttgagaaccc cgccggccga
     1501 gagcccgaga aaaacgtcca cgcccggcat gatctccgcg agcgtacgcg ccgatgtcct
     1561 gcgcgcgtag cgagccttgt tcgggtccat ttcctctttt cggccctggt acacgacgcc
     1621 cttgatatcg gacacccaga tgttgtccgg cttcaacccc aggctcacga gcagatcgag
     1681 acaagcgagc gcagcggcac ccgcgcccga gcagacgagc ttcgcctttg ccatgtcctt
     1741 ccccacgacg cgcaatccgt tgagcaccgc agcgccgacg atgatggcgg tgccgtgctg
     1801 gtcgtcgtgg aacaccggga ttttcatgcg ctcgcgcaac ttcttctcga tgtggaagca
     1861 ttccggcgcc ttgatgtcct cgagattgat tccgccgaaa gtgggctcca gggccgcgat
     1921 gatctcgacc aacttgtccg gatcgcgctc attcacctcg atgtcgaagc aatcgattcc
     1981 ggcgaacttc ttgaagagca ccgacttgcc ttccatcacc ggcttggacg cgagcggccc
     2041 gattgccccc aaccccaaca cggcggtgcc atttgtcacg acaccgataa ggtttccgcg
     2101 cgaagtcagc ttgcgcgcct cggctgggtc gcgcacgatc tcctcgcagg ccgcagccac
     2161 cccgggcgtg tacgcgagcg cgagatcgcg ctggttcagc aggcccttgg tgggagtgac
     2221 tgaaatcttt cccggcggtt gccggaggtg atactccagg gcgctcctgc gcaggatttc
     2281 atccatggga aattagcgac ttgaagttct tatgctgctt ggccgcatga tagccgaatt
     2341 tagcggtgcc gcggcgcctt cgcatccgtg ccagccctcc gcgttcaact tgcgacgcgc
     2401 tgcggcataa tctcccgatg ccaaacgcga atccggtcct cgcggcccgc atggccgata
     2461 tcgaagcctt ccatgtaatg gacgtgcaaa accgcgcgca cgagctcgaa gctgcgggac
     2521 ggcgcatcgt gcacatggaa atcggccagc cggactttgc ggcgccgcct ctggtcgcag
     2581 aggctgcgat cgaggcgatc cgccagcggc gcctgggtta caccgcttcg ataggcattc
     2641 cggagctgcg ccaggccatt tccgatcact atcgggaacg gctcggcgtg acggtaccct
     2701 cttcgcggat cgtagtcacc gcaggagcat cgggcgcctt cttgctcacg ctcggtgcgt
     2761 tggtgaaccc cggggacgag gtgctgatgc ccgacccctg ctatccctgc aaccggcatt
     2821 tcgtgcgctt gctcgaaggc cgcccccgcg cagttccagt cgaggagaac cagcactacc
     2881 aacttgccga cgctgatatt cgttcgcact ggagccgggc gacgcgcggc gtgctgctcg
     2941 cttcaccttc caatccgacc ggaaccatga tcccgcggga ggagctgcgc gcgatcatcg
     3001 cctcggtgcg acgggcgggc gggttcgtcg taatggacga gatctatcag ggactggtct
     3061 acgacggtga ggcgtcgacc gtgctcgagc tggccgatga tgtgttcgtc gtcaacagct
     3121 tttccaagta cttcaacatg acgggctgga ggctgggctg gatcgtcgct cccgaggcct
     3181 acgtgcggga gatcgagaag ctcgcccaga acgcgttcat ttgcccttcc gcccccgcgc
     3241 agtacgccgc gctcgcggcg ttccggccgg aaacactcgc ggttctggag gagcgcaggc
     3301 gggagttccg gcgccgccgc gattacatgg ttcccgcgct gcgggcgctg gggtttcgca
     3361 ttccccttat gccccaaggg gccttctaca tctatgccgg atgcgagagg ttctccgagg
     3421 acagcaccca attcgcattg cgggtgctgg aagaagccgg ggttgcaatt acgccggggc
     3481 tagacttcgg ctcgaaccgc ccggagcgcc acgtgcgctt cgcctacacg cgctcgctcg
     3541 aggatctcaa ggaaggagtg gaaaggatcg cgcggatgct ggggggcggc gacgccccgc
     3601 ggtcatactg agctgcgcgc ccccgggcga ccgaattggc gtaccgcctg ctgcaagcgc
     3661 tccttttcgg cgaagacttt ctgcgcatat tggctcgaag gatccgccaa tgcgccgtta
     3721 tagaactgca ggcccgcctc gaggctgccg gtgcggcgaa cgtattcctt gagaatgcgc
     3781 gctcccaacg cgatgttgat catcggatcg agcaccactt cttctccgcc gtgctccagc
     3841 agcttgtcga ggtgatattt gggaatcacc tgcatcaggc ctctggcgcc caccaggctc
     3901 tcggctaccg ggttgaagcg agactcgacc gccataaccg cgagaatcag caggggatcc
     3961 aggcccacct gccgaccggc gtcgtaggct gcacccacaa actgctcgga gacatccggc
     4021 gcgatccggt atctacggga gacgtaattc gccagcgccc ggtatttctg cgtgtccgcg
     4081 tcgtccgccc cttcgaacag ggagaccgct ccatcgtagc ggatcgagcc gactgccgca
     4141 gaacggctgg cgtcctcgtc gaagaacagg gtcttcccgc gcacgaggat gatcaccacg
     4201 gccgcgagcc ctatgaaggc cagaccatga tgaagtatcg tcgccactct tccaaactga
     4261 tccagcaaac cttgccttgt ccaggtggtg ttcattgcac ctacctccat tcttttgctt
     4321 ccgaagagca gataaagctc ttggtaaccg accccgccct tgaagccttt agacgcttct
     4381 gtctgtttct cgattatagc aagggtttgc tgcaaggcag caaaccctat tagaataggc
     4441 acttaacaaa atcacaaacc ccaagaccca aacgatcaag caactgttga gcccgatcct
     4501 cctcgtctgc gtccgaacag ccacggctca gtccggggca caaaggcccc cggactgacc
     4561 gcggttcact cccaacccgc ttatggcctg gaacccgtcg ggaagggcca tgcagccgcg
     4621 ggattcaggg cggttttcgc ccctcctagc gtcgccgctg ccgaaggggc tgtggccgcc
     4681 gatcccgcca tcgttgctgc cggcgttgcg gctttcctgg ccgcccttcc tctgggtctc
     4741 gccttctttc gggcggattt tttcttggcc gctttttttg cgggcttctt tagcgacttc
     4801 cttttccctt tctttcgtgc tgctttcctc tttttctttt tctttgccgc tttcttgcct
     4861 tttctcttct ttgccatggc tggcctccat caagagttgt gaaacaaaga gcccgcccgt
     4921 tggggcgcgg gctatccaat ctccggggca agccccggag aattgaattc ttctcctgct
     4981 ctgccgattc gaccgaagct gcaggcgctt agtcccaact gagcgcacct ccggtttggt
     5041 actcgatcac ccgggtttcg aaaaaattcc gctccttctt gagatcggtc atctcgctca
     5101 tccaaggaaa cgggttgctc gcaccggaaa acatcgcatc cacaccgatc tgctggcaac
     5161 ggcgattggc gatgtagcgc aggtactcct tgaacatcgg agcgttgagc cccaggacac
     5221 cgcgcggcat ggtgtcctcg gcatagcgat actcgagctc gaccgctttc ttgatgaggc
     5281 tctggatctc ctcgcggaac tcgggggtcc acaagtgcgg gttctccatc ttgatctggt
     5341 tgataaggtc gatgccgaaa ttgcagtgca tcgactcgtc gcgcaggatg tactgatact
     5401 gctcggcgga gccggtcatc ttgttctgcc ggcccagcgc cagaatctgg gtgaaaccga
     5461 catagaagaa catgccctcc atcacgcagg cgaacacgat gaggctgcgc agcagctttt
     5521 gatcggcctc gggcgtgccg gtccggaagg tcgggtcggt gagagtgtcg atgaaaggaa
     5581 tgaggaactc gtccttgtca cggatgctcg ggatctcgtg ataggcgttg aatacctcgc
     5641 cctcgtccag acgcaagctc tcgacgatgt actggtaggc gtgagtatgg atagcctcct
     5701 cgaacgcctg ccgcaggagg tattgccggc attcgggcgc ggtgatgtga cggtaggtac
     5761 cgagaacgat gttgttcgca gcgagggaat ctgccgtgac gaagaagccc aggttgcgct
     5821 tgactaggcg tcgctcgtcc tccgtcagcc cgttcgcgtc tttccacagg gcgatgtctc
     5881 ggctcatgtt gacttcctgc ggcatccagt gattggcgca ggcggcgagg tatttttccc
     5941 acgcccattt gtacttgaac ggcacgagct gattcacgtc cgcccgggcg ttgatgatgg
     6001 ccttgtcctc gacgcgcacg cgcctctggc tgttgccggc ggaatagcgt tccgtcggga
     6061 ccgcggtcgg caccgactcc agcggcatca acccgctcac cgtttgcagg ttgaggcccg
     6121 gaatcgcctc ggccccgatg tcatcaaaag aaagcattct gtccctcccc gttctcgtgc
     6181 ttgttagcgc tcactggcag gattcgcact ccggatcgtc gatcttgcac aacttgattt
     6241 ccggctcgtc tgatgcacgc gaagcggctg caacggtaga tttcgccgcc ggcatccctc
     6301 cttcgggaac ggcgttcagt tgaccggctc tcaccgtgga tttctcggcg tgcgtcgcgc
     6361 ccagggcgcg caggtagtaa gtggttttca agccccgctg ccaggcgagc ttgtagcttt
     6421 cgtccagctt cttgccggaa gccttgccga tgtagatgtt gagggactga ctggtcgatc
     6481 catttctgcc gccgcgcgcc ggcttcgacg acccacacgg gttcgacttc gaatgcggtg
     6541 gcg
//
LOCUS       DASPZD010000489         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1111534, whole genome shotgun sequence.
ACCESSION   DASPZD010000489 DASPZD010000000
VERSION     DASPZD010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111534"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VEM33_09570"
     CDS             <1..267
                     /locus_tag="VEM33_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYS52117.1"
                     /translation="GLALAALGIPDESQYIASYCGRTGREGIEHWDFYLAYNLFRIAA
                     ILQGVMKRALEGTAASAQALEAGRRARPLAELGWKYAEKAMLNP"
     gene            280..1512
                     /locus_tag="VEM33_09575"
     CDS             280..1512
                     /locus_tag="VEM33_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYS52118.1"
                     /translation="MDFAYSEKVKALQAKLARFMDAHVYPNERRFKDEVEANRAKGNP
                     WVPTKLIEELKPKARAEGLWNLFLPRSPRAPEGLSNLEYAPLCEIMGRVHWAPEVFNC
                     AAPDTGNMETLERYGSEEQKRHWLDPLLRGEIRSAFLMTEPDVASSDAANIQCRIERN
                     GNDYVVSGRKWWSSGAGDPRCKVFIVMGKTDPGAAKYSQQSMILVPREASGVKILRPL
                     SLFGYDDAPHGHMDIALENVRVPASNILLGEGRGFEIAQGRLGPGRIHHCMRLVGVAE
                     RALELMCKRGLERVTFGKSVAERTVTQERIAEARIMIESARLLVLKAAHMMDTVGNKA
                     ARAEIAMIKVLAPNVACQVLDWAIQAHGGGGMSEDFPLAYMYAHSRTLRFADGPDEVH
                     RNAIAKLELSKHLPAPKR"
     gene            complement(1539..1892)
                     /locus_tag="VEM33_09580"
     CDS             complement(1539..1892)
                     /locus_tag="VEM33_09580"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HYS52119.1"
                     /translation="MPADANQHGDIFGGWIMAQVDIAGSVLAARHARGRVATVAVNSF
                     QFKQPVFIGDVLSFYGEIVRVGNTSITVNAEVYAQRNPSDLETVKVTEATLTYVAMGP
                     DRKPRPVPPLPDRKG"
     gene            complement(1950..>2213)
                     /locus_tag="VEM33_09585"
     CDS             complement(1950..>2213)
                     /locus_tag="VEM33_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYS52120.1"
                     /translation="AGKPVLLLPTQLEQFLIMRRLVRFGAGLGIAPEVSNADYASALK
                     ALAEKGQYAAKAREFADRYAGHARGAALATMTARIEAALQPSR"
BASE COUNT          394 a          694 c          757 g          368 t
ORIGIN      
        1 ggtctggccc tcgccgcgtt ggggattccg gacgagtcgc aatacattgc gtcgtactgc
       61 gggcgcaccg ggcgcgaggg gatagagcac tgggatttct atctcgccta caatctcttc
      121 cggatcgccg cgatccttca gggcgtgatg aaacgcgcgc tggagggaac ggcggcgagc
      181 gcccaggcgc tcgaggcggg ccggcgagcc cgtccactgg cggaactggg ctggaaatac
      241 gccgaaaaag caatgctgaa tccctagcac gagggccgaa tggattttgc gtactcggaa
      301 aaggtgaagg ctctgcaggc gaagcttgcg cgcttcatgg atgcacacgt ctatccgaac
      361 gagcgccgct tcaaggacga ggtcgaagcg aaccgcgcga agggcaatcc ctgggtgccc
      421 acgaagctca tcgaggagct gaagccgaag gcgcgcgccg aaggactctg gaatcttttc
      481 ctgccgcgct cgccgcgggc gcccgaggga ctctccaacc tcgaatacgc gccgctgtgc
      541 gaaatcatgg ggcgcgtgca ctgggcgccg gaagtgttca actgcgccgc gcccgacacc
      601 ggcaacatgg agacgctgga gcggtacggc tccgaagagc agaagaggca ctggctcgat
      661 ccactgcttc gcggagagat ccgctcggcc ttcctcatga ccgaaccgga tgtcgcttcg
      721 tccgacgcag ccaacattca gtgccgcatc gagcgaaacg gaaacgatta cgtcgtcagc
      781 ggccgcaaat ggtggtcatc gggcgcgggt gatccgcgct gcaaggtctt catcgtgatg
      841 ggcaagaccg atccgggcgc ggccaaatac tcgcagcagt cgatgatcct cgtgccgcgc
      901 gaggcgtccg gggtgaagat cctgcgtccc ttgagcctct ttggctacga cgacgcgcct
      961 cacggccaca tggacatcgc tttggagaac gtgcgcgtgc cggcttcaaa catactgctc
     1021 ggagagggcc gcggcttcga gatcgctcag gggaggctcg gtcccgggcg catccaccac
     1081 tgcatgcgcc tggtcggcgt ggccgagcgc gcgctcgaat tgatgtgcaa gcgcggcctg
     1141 gagcgggtca ccttcggcaa gtccgtcgcg gagcgcacgg tgacgcagga gcgcatcgcc
     1201 gaggcgcgca tcatgatcga atcggcgcgc ctgctcgtgc tgaaggccgc ccacatgatg
     1261 gacaccgtgg gaaacaaggc ggcgcgagcc gagatcgcga tgatcaaggt gctcgcgccc
     1321 aacgtcgcgt gccaggtgct cgactgggcg atccaggcgc acggcggcgg cgggatgtcg
     1381 gaggattttc cgctcgccta catgtacgcc cattcgcgca cgctgcgctt cgccgacggg
     1441 cccgacgagg tccaccgcaa cgcgatcgcc aagctcgagc tctcgaagca cctgccggcg
     1501 ccaaagcgct gagctcagca ttgcccccgc tggatccgtc atcccttccg atcgggaagg
     1561 ggcggcacgg ggcgcggctt gcggtcaggg cccatggcga cgtaggtgag cgtcgcctcg
     1621 gtcaccttga cggtctcgag gtcggaggga ttgcgttgcg cgtagacctc cgcgttgacc
     1681 gtgatcgagg tgttgcccac gcgcacgatc tcgccgtaga aggaaagcac gtcgccgatg
     1741 aatacgggct gcttgaactg gaacgagttg accgcgacgg tggcgacgcg gccgcgcgcg
     1801 tggcgcgcgg cgagcacgct tccggcgata tcgacctgcg ccatgatcca gccgccgaag
     1861 atgtcgccgt gctggttggc atcggccggc atcggcacga cgcggaggat ggggtccttg
     1921 ttttcaggca gcttggtcac cgtgcaatgt taccgtgaag gctgaagcgc cgcctcgatg
     1981 cgcgcggtca tggtggcgag cgccgcgccc cttgcatggc cggcatatcg atcggcgaac
     2041 tcgcgcgcct ttgcggcgta ttgccccttt tccgcaagcg ccttgagcgc ggacgcatag
     2101 tcggcattgg acacttccgg tgcgatgccc agacccgcgc cgaagcgcac caggcgcctc
     2161 atgatgagaa attgctcgag ttgggtcggc aggagcagca ccggttttcc tgc
//
LOCUS       DASPZD010000490         5226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_179845, whole genome shotgun sequence.
ACCESSION   DASPZD010000490 DASPZD010000000
VERSION     DASPZD010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179845"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VEM33_09590"
     CDS             complement(<1..645)
                     /locus_tag="VEM33_09590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS52121.1"
                     /translation="MLTRRDVLIGAIAAGATMHLRTGFAKASQPATRVNFDVPAGACD
                     CHTHIHGDPEKFPFFPGRVYTPEAALPEEMAALHRALRMQRVVIVTPSVYGTDNAATL
                     FGMRARGADARGVAVIDDKTPESELDAMGREGVCGIRLNLSTGGTNDPVVGRRRFKEA
                     AERVKTRNWHVQMNTNLAMVSATKDLVAASPVPIVFDHFANAREQLGVQQPGFAD"
     gene            complement(668..2263)
                     /locus_tag="VEM33_09595"
     CDS             complement(668..2263)
                     /locus_tag="VEM33_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYS52122.1"
                     /translation="MDADVLIVGGGPTGLMLANQLGRREIRATIVDRHSGPAQQTRAM
                     AVHARTLEIYSKLGIAERALELGRRGNGANMWAEGKWAARIPLGDIGKSISPFPFVLM
                     LGQDDNERIMGDHLRNWGMAVQWNTELTGIRQESGHVAATLKQPDGSTREVQAAYVAG
                     CDGGHSAVRELCGITFPGAAYEHVFFVADTEATGPMVAEELNVYLWREGFHLFFPMRG
                     KDRWRVIGILPKKLQGRDDVTFEELSPDIRREAGANLVFKECSWFYIYHIHHRAAEKF
                     RDRRCFLLGDAAHVHSPAGGQGMNTGLQDAYNLGWKLALVVKGHADAALLDSYEAERI
                     PVAQRLLATTDRAFMLVVSDSWLAGLFRTRILARIVAFVMTFERPKQLAFRTVSQTGI
                     AYRESPLSKMLRGLPKGAPRAGDRFPWLQLRLRENGPIEDLFQKLDDRRYNLIVIGQP
                     APPGEAQGLGDLLRIHAIPDDPHNAQELARVRIPGPAFYLLRPDGHVGLAGTRLEPDA
                     VTRYLSESCIRIQGKIPKPVRNG"
     gene            complement(2336..3571)
                     /locus_tag="VEM33_09600"
     CDS             complement(2336..3571)
                     /locus_tag="VEM33_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYS52123.1"
                     /translation="MITPGVPMRRIAAFAAVVLLAGSAIPAFAQVSAYEGARLIVGDG
                     RVVENATLVVDGAKLAQAGAAADVRVPSGARRVDLRGRTVMPMIVDTHVHLSTTRDAL
                     LRDLRARAYWGVSAVLSMGTDGYEVLGVHNEVIPGAARYFSAGRGITTPEPGRTTAPY
                     WITSATEGRKAVAELAAHKVDIVKIWVDTREGKYKKLTPEMYGAIIDEAHKRSLRVTA
                     HLFDLEDAKGLVRAGVDVLAHGVRDKDIDDEFVAMFKAHPNLVLTPNLPERGVKVDLG
                     WMKAGLSPEDFAKLEKANVDNPKAQAFYGIQARNLAKLNAAGARIVLGSDGNRPWGPH
                     EEMQDMVVAGMTPTQVIVAATRNGAELLRMDAGTLVAGKSADFIVLDANPLDDIANTR
                     RISQVIMRGAPVDRSQPVR"
     gene            3638..4612
                     /locus_tag="VEM33_09605"
     CDS             3638..4612
                     /locus_tag="VEM33_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HYS52124.1"
                     /translation="MLFIARRALAVVLLMVASMAAWAQGYPDRPLRLVVGFPPGGSGD
                     FLARIIADELTREIDQPVVVDNKPGAGSNIAAEHVARAASDGYTILLAGNFTHAINPW
                     LYRNLSWDPHRDFTPITEVALMSIIVCVTPERGIRSMKELIARVRSEPGKWFYASPGN
                     GTSQHLAGAELNRITGTDMQHVPFKGGAPSLQAVLAGDVQVMIGTTPVVLPQIRAGKL
                     VPLALITRAASRMVPGVPGMEEAGVPGLDLGSWWGIWAPAGIRPEVKQRLYATITKVM
                     RLPQVAEKLAREAMEPEISVSPEEFAAFVRRDHASYAPIVKSSGAKAD"
     gene            4638..>5226
                     /locus_tag="VEM33_09610"
     CDS             4638..>5226
                     /locus_tag="VEM33_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYS52125.1"
                     /translation="MQLKKLGRDFALEASGVDLARPLSDGAFREIEEAFFAGQLLVFR
                     AQKLTAREFLGFARRFGPPEPHVVDQFHHPEYADILILSNVVRDGKPAGLADAGTYFH
                     TDYSYLEMPARATLLYSIQVPRAGGDTLFANQYAAYADLPGAMKKKLEGLIGLHHYGN
                     RDDLEETSRTAASLLTEEQKRRLQWVRHPVARRHPV"
BASE COUNT          811 a         1752 c         1743 g          920 t
ORIGIN      
        1 gtcggcgaag ccgggttgtt gcacgccgag ctgctcccgc gcgttggcga aatgatcgaa
       61 cacgatcggc acgggcgagg cggcgaccag gtccttggtc gcggaaacca tcgccaggtt
      121 ggtattcatc tgaacgtgcc agttgcgggt cttgacgcgc tcggcagcct ccttgaaccg
      181 ccggcggccg acaaccgggt cgttggtgcc gccggtcgag agattgagcc ggatgccgca
      241 cacgccctcg cggcccatgg cgtcgagctc gctctcgggc gtcttgtcgt cgatgacggc
      301 gacacctcgt gcgtcggcgc ctcgtgctct cattccgaag agagtcgcgg cattgtcagt
      361 gccgtataca gaaggcgtga cgatcaccac gcgctgcatg cgaagcgcgc ggtgcagcgc
      421 cgccatctcc tcaggcagcg ccgcctcggg cgtatagacg cggcccggga agaacggaaa
      481 tttctccggg tcgccgtgga tatgggtgtg gcagtcgcag gcacccgccg gcacgtcgaa
      541 attcacccgc gtggccggct gcgaggcttt ggcaaaaccg gttcgaaggt gcatcgtcgc
      601 tccggcggca atcgctccaa tcaggacgtc gcgtcgcgtc agcatggagt cctccttcgg
      661 tggaatctta tccgttgcgc accggtttcg gtattttccc ttgaatccga atgcagcttt
      721 cggacaggta ccgggtgacg gcatccggtt ccaagcgagt cccggcgagc ccgacgtggc
      781 cgtcgggacg cagcaaatag aaagccggtc ccgggatccg aacgcgtgcg agctcttgcg
      841 cgttgtgcgg atcgtcggga atcgcgtgga tgcgcaggag atcgccgagt ccctgcgctt
      901 ctcccggtgg cgcgggctgc ccgatgacga tcaggttgta gcgcctgtcg tcgagcttct
      961 ggaacagatc ctcgatcgga ccgttttccc gcagcctgag ttgcagccac ggaaagcggt
     1021 cgcccgcgcg cggggcgcct ttgggcaggc cccgcaacat tttcgacagc gggctttccc
     1081 ggtaggcgat gccggtctgg gagacggtcc ggaacgcgag ctgcttcggc cgctcgaaag
     1141 tcatcacaaa ggccacgatt ctcgcgagga tgcgcgtgcg gaaaagcccc gcgagccagc
     1201 tgtcggaaac caccagcatg aatgcccggt cggtcgtggc caacaagcgc tgcgcgaccg
     1261 ggatacgctc ggcctcatag ctgtcgagca gcgcggcgtc cgcatggccc ttgaccacga
     1321 gcgcgagctt ccaccccagg ttgtacgcat cctgcagccc ggtattcatg ccctgtccgc
     1381 cagccgggct gtggacgtgc gccgcgtcac cgagcaggaa gcagcgccgg tcgcggaact
     1441 tctccgcggc gcggtgatgg atgtggtaga tgtagaacca gctgcattcc ttgaagacaa
     1501 ggttggctcc cgcctcccgg cgtatatcgg gactcagctc ctcgaacgtg acgtcgtctc
     1561 tgccttgcag cttcttgggg aggatgccga tcacccgcca gcgatccttg ccgcgcatcg
     1621 ggaagaacag gtgaaatcct tcgcgccaca ggtatacgtt cagttcttcc gcgaccatgg
     1681 ggcccgtcgc ctcggtatcg gcgacgaaga acacgtgctc gtacgccgcg ccgggaaagg
     1741 tgatgccgca gagctcgcgc accgcgctgt gcccgccgtc gcagcccgcg acgtacgcgg
     1801 cctggacctc gcgggtggag ccatccggct gcttgagcgt tgcggctacg tgaccggatt
     1861 cctgcctgat gcccgtgagc tcggtgttcc actgcaccgc catgccccag ttgcggaggt
     1921 ggtcgcccat gatccgctcg ttgtcgtcct ggcccagcat caggacgaag ggaaacgggc
     1981 tgatgctctt gccgatgtcg cccagcggga tgcgcgctgc ccactttcct tccgcccaca
     2041 tattggcgcc gttgccacgg cggcccagct cgagcgcccg ctccgcgatc ccgagcttgg
     2101 agtagatctc gagcgtgcgc gcatgcaccg ccatcgcccg cgtctgctgc gccggcccgg
     2161 aatgccgatc cacgatcgtc gcgcggattt cgcggcgccc gagttgattg gcgagcatca
     2221 gcccggtggg gccgcctccc acgatcagca catccgcatc catgcgactc tgcttcagac
     2281 gaggggttcg ctagtgtgcg ctcaggccaa gacgatcgcc gccccccgga gagccctagc
     2341 ggaccggctg cgagcggtcc accggcgcgc cgcgcatgat cacctgcgag atgcgccgcg
     2401 tgttggcgat gtcgtccagt gggttcgcgt cgagcacgat gaagtcggcg ctcttgccgg
     2461 cgaccagcgt gccggcgtcc atgcgcagga gctcggcgcc gttgcgcgtc gccgccacga
     2521 tgacctgcgt cggcgtcatg ccggcgacta ccatgtcctg catctcctcg tgcggacccc
     2581 agggccgatt gccgtcgctt ccgagcacga tgcgcgcgcc cgccgcgttc agcttggcca
     2641 ggttgcgcgc ctggatgccg tagaaggcct gtgccttcgg attgtcgacg ttcgccttct
     2701 ccagcttcgc gaagtcctcc ggggagaggc cggccttcat ccagcccagg tcgaccttca
     2761 cgccgcgctc cggaaggttc ggcgtgagga ccaggttcgg atgcgccttg aacatggcga
     2821 cgaactcgtc gtcgatatcc ttgtcgcgaa ctccgtgcgc gagcacgtcg acgcccgcgc
     2881 gcacgaggcc cttcgcgtcc tcgagatcga agaggtgcgc cgtcaccctc aagctccgct
     2941 tgtgcgcctc gtcgattatt gcgccgtaca tttcaggggt cagctttttg tacttgcctt
     3001 ccctcgtgtc gacccagatc ttgacgatgt ccactttgtg cgccgcgagc tcggccaccg
     3061 ccttgcggcc ttctgtcgca ctggtgatcc agtagggcgc ggtcgtgcgc cccggctcgg
     3121 gcgtggtgat gccgcgcccc gcgctgaagt accgcgcggc tccgggaatc acctcattgt
     3181 gcacgccgag cacctcgtag ccgtccgtgc ccatgctcag gacggcgctt acgccccaat
     3241 aggcccgcgc tctcaggtct cgcaggagcg cgtcgcgcgt agtgctcagg tggacgtgcg
     3301 tgtcgacgat catcggcatc accgtcctgc ccctgagatc gacgcgcctc gcgcccgagg
     3361 gtacgcgcac gtcggcggca gcgcccgcct gcgcgagctt ggcgccgtcc acgaccagcg
     3421 tggcgttctc gaccacgcgg ccgtcgccga cgatgaggcg cgcgccttcg tacgcgctga
     3481 cctgggcgaa cgccgggatc gcggatccgg cgaggaggac gactgctgcg aatgcggcga
     3541 tgcggcgcat gggaaccccc ggggtgatca tttgaaatat ggcgtgccat gataccgtat
     3601 ggccgcaggc ggtaaggtcc agctgaagga ggcttccatg ctgttcatcg cgcgtcgcgc
     3661 cctggccgtg gtcctgctca tggtcgcttc gatggcggcg tgggctcaag gctatccgga
     3721 ccgtccgctg cggctcgtgg tcggatttcc tcccggtgga agcggcgatt ttctcgcgcg
     3781 catcatcgcc gacgagctga cgcgggaaat cgaccagccg gtggtggtgg acaacaagcc
     3841 cggcgcaggc tccaacatcg ccgcggagca cgtcgcgcgc gccgcatccg acggctatac
     3901 catcctgctc gccggcaatt tcacgcacgc gatcaatccc tggctgtaca ggaacctctc
     3961 gtgggatccc catcgcgact tcacgcccat caccgaggtc gctctgatga gcatcatcgt
     4021 gtgcgtcacg cccgagcgcg ggatccgctc gatgaaggag ctgatcgcca gggtcaggag
     4081 cgagcccggc aagtggttct acgcctcgcc cggcaacggc acctcgcagc acctcgcggg
     4141 cgcggagctg aaccgcatca ccggcaccga catgcagcac gtgcctttca agggcggcgc
     4201 gccgtcgctg caggcggtgc tcgcgggcga cgtgcaggtg atgatcggca cgacgccggt
     4261 ggtgctcccg cagatccgcg cgggcaagct cgtgccgctc gcgctgatca cgcgcgcggc
     4321 ctcgaggatg gttccggggg tgcccgggat ggaggaggcg ggcgtgccgg ggctggacct
     4381 ggggagctgg tggggcatct gggcgccggc cgggatccgg cccgaggtga agcagcgcct
     4441 gtatgcgacc atcaccaagg tcatgcgcct gccgcaggtc gcggagaaac tcgcgcgcga
     4501 ggcgatggag cccgaaatct ccgtctcgcc cgaagaattc gccgcgttcg tgcgcaggga
     4561 ccacgcttcc tacgcgccca tcgtcaagtc ttcgggcgca aaggcggatt gagccggcga
     4621 aggcccgcgg tgcaacgatg cagctgaaaa aactcggccg ggatttcgcg ctggaggcga
     4681 gcggtgtcga tctcgcccgg ccgctttccg acggcgcttt tcgcgaaatc gaagaagcgt
     4741 tcttcgccgg ccagctgctc gtgtttcgcg cgcagaagct gactgcgcgc gagtttctcg
     4801 gattcgcgcg ccgcttcggg ccgcccgagc cgcacgtggt cgaccagttc caccatcccg
     4861 aatacgccga catcctgatc ctgtcgaacg tggtgcgcga cggcaagccg gccgggctcg
     4921 ccgacgccgg gacttatttc cacaccgact attcgtatct ggagatgccg gcgcgggcga
     4981 cgctgctgta ctcgatccag gtgccgcggg cgggcggcga tacgctgttt gcgaaccagt
     5041 acgccgccta cgccgatctg cccggcgcga tgaaaaaaaa gctggagggg ctgatcgggc
     5101 tgcatcacta cggcaaccgc gacgatctcg aggagacctc gcgcacggcg gcctcgctgc
     5161 tgaccgagga gcagaagcgc aggctccagt gggtgaggca tccggttgcg cggcgccatc
     5221 ccgtca
//
LOCUS       DASPZD010000491         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U11610
            k141_1507924, whole genome shotgun sequence.
ACCESSION   DASPZD010000491 DASPZD010000000
VERSION     DASPZD010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945340
            Sequence Read Archive: SRR6266576
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:37:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,924
            CDSs (total)                      :: 1,907
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 17
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 14
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 16 of 23
            Pseudo Genes (internal stop)      :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507924"
                     /isolate="SMAG_U11610"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.5673 N 123.4758 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VEM33_09615"
     CDS             complement(<1..938)
                     /locus_tag="VEM33_09615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYS52126.1"
                     /translation="MSSGQLLTACRLAWEQGGQLAALWASDERDRERGFCVHVLLRDR
                     DGLTLLEHTLPDGGARYPDLSTIFPVANRMQRAAFDLVGTECDTDDPRPWIWQAAWPI
                     DQFPLRRDFAASPKWEPGEEDYPFVRVSGDGVHEIAVGPVHAGTIEPGHFRFQVVGEK
                     VLRLEERLSFAHKGIEKRFETLSIADGSRLAARISGDSAVAYGWAYAQAVEAIAGLEL
                     SQRATWLRALCLERERVANHLGDLGYLGNDGGFAFGLSQFSRLKEDVLRLNRRVFGHR
                     LLMDVIVPGGVTHDIAPESTAEMRTQCASLDREIRLL"
     gene            complement(1027..2475)
                     /locus_tag="VEM33_09620"
     CDS             complement(1027..2475)
                     /locus_tag="VEM33_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HYS52127.1"
                     /translation="MEILAVLGVPVAGALVLAAIGHRDQAAMVNVCVCLATFLSAVAL
                     TVRVISHGPLLVLDRLFFVDPFNVFLVALTAFVGFTTSLFSRPYMRIEHEHGRLTPAR
                     LRLYHSMFQVFNFTMLLALLTNNMGILWVALEAATLATVLLVSLYRTPASLEAAWKYF
                     ILCGVGIAQALFGTILLYFAAEKLLGVEGGALLWTELNAVKGQLEPTVLSLAFVFLLV
                     GYGTKVGLVPLHNWLPDAHAEGPTPVSAVLSGLLLNVALYAVVRCKVLVDGSLQTSFA
                     KGLMMGFGMLSVVVAAFLLSRQKDIKRLFAYSSIEHMGIITFAFGMGGPIANFAGLLH
                     MTVHSLTKSAIFFAVGHAAQKTGTQLMDGIRGLITISPAIGWGLVLGTLAILGMPPFG
                     VFASEFLILTTAMHEQPWATPLLLLSLGVAFAAVFSKVQPMVFGETTAKQLPHRPMMA
                     PVFVHMALVLMLGLWIPPFLADWYRQAAKLIG"
BASE COUNT          534 a          792 c          799 g          384 t
ORIGIN      
        1 aggaggcgaa tctcgcgatc gagcgaggcg cattgcgtgc gcatttcggc ggtggattcg
       61 ggcgcgatat cgtgcgttac gccgcccgga acgattacat ccatcagcag gcggtgcccg
      121 aacacgcggc ggttgaggcg cagcacatcc tctttcaaac gcgagaattg cgaaaggccg
      181 aacgcgaatc cgccgtcgtt gcccagatac cccaagtcgc ccagatggtt ggcaacccgt
      241 tcgcgctcga ggcacagcgc gcgcagccac gtggcgcgct ggctaagctc gaggcctgcg
      301 atcgcctcga ccgcttgcgc gtaggcccag ccgtacgcga ccgcgctatc gcccgaaatc
      361 ctggcggcga gacgggaccc gtcggcgatc gacagcgtct cgaaacgttt ttcgatgccc
      421 ttgtgcgcaa aactcagccg ctcttcgaga cggagcactt tctcccccac tacctggaag
      481 cgaaaatgtc cgggctcgat cgtccccgca tgcaccggcc cgacggcgat ttcgtgcact
      541 ccgtcgccgg acacgcgcac aaagggatag tcttcctcgc ctggctccca tttcggcgac
      601 gcggcgaagt ctcgccgcag cggaaattga tcgatcggcc atgctgcctg ccagatccag
      661 ggccgcgggt cgtcggtgtc gcactcggtg ccgaccagat cgaacgctgc gcgttgcatc
      721 cgattcgcta ccggaaagat tgtcgacagg tcgggatagc gcgccccgcc gtcggggagc
      781 gtgtgctcga gcagggtcag gccgtcgcgg tcgcgcaaca gaacgtgcac gcaaaagcct
      841 cgctcgcggt cgcgctcgtc gctcgcccag agcgccgcca gttgcccgcc ctgttcccac
      901 gcgagccggc acgccgtcag caactggccc gagctcacgg ggcagcgctg cgcaggaatc
      961 ggagaggcca aagtcgcgga agacacaccc agaggcgaga cgttcatcga agctccaagt
     1021 gtgcgattag ccgatgagtt tcgcagcctg gcggtaccag tcggcgagga agggtgggat
     1081 ccagaggccc agcatgagca cgagggccat atgcacgaac accggagcca tcatcggccg
     1141 atgcggcaac tgcttggcgg tggtttcccc gaacaccata ggctgtactt tggaaaatac
     1201 cgcggcgaac gcgacgccga gcgaaagcaa cagcagtggc gtagcccagg gctgctcgtg
     1261 catcgccgtg gtcaggatca gaaactcgct ggcgaagacg ccgaaaggag gcatgccgag
     1321 gatcgcgagc gtccccagga ccagtcccca gccaatggcg ggactgatgg tgatcaggcc
     1381 gcggatgccg tccatgagct gggtgccggt cttctgcgcg gcgtgaccca ccgcgaaaaa
     1441 aatcgccgac ttggtcagcg agtgcaccgt catatgcaag aggcccgcga agttggcgat
     1501 cgggccaccc atgccgaagg cgaacgtgat aatgcccatg tgctcgatcg acgagtaggc
     1561 gaacagccgc ttgatgtcct tctggcgcga cagcagaaac gccgcgacga ccaccgacag
     1621 catgccgaag cccatcatga gtcccttggc gaaggaggtc tgcagcgagc cgtcaacgag
     1681 gactttgcaa cggaccaccg catagagcgc gacattgagc agcaatcccg agagcacggc
     1741 cgatacgggc gtcgggccct cggcgtgagc atccggcagc cagttgtgca atggaacgag
     1801 tccgaccttg gtgccgtatc cgacgagcag gaacacgaac gcgagcgaga gcacggtggg
     1861 ctcgagctga cccttaacgg cgttgagctc ggtccacagc agcgcacctc cctccacgcc
     1921 cagaagcttt tccgccgcga aatagagcag gatggtgccg aacagcgcct gcgcaatgcc
     1981 gaccccgcaa agaatgaaat acttccaggc ggcctcgaga cttgccggcg tgcggtagag
     2041 cgataccagc agcaccgtcg caagtgttgc cgcctccagc gcgacccaaa ggatgcccat
     2101 gttattggtc aagagcgcca gcagcatggt gaaattgaac acctggaaca tgctgtggta
     2161 gaggcgcaat cgtgcgggcg tcagacgtcc gtgctcgtgc tcgattctca tgtacggcct
     2221 ggagaatagc gacgtggtga agccgacaaa tgcggtgagc gctaccagaa acacgttgaa
     2281 gggatcgacg aagaacaagc ggtcgagcac cagcagcggg ccgtgggaaa tcacgcgcac
     2341 ggtcagcgcg accgcagaaa gaaacgtggc caggcacacg cacacattga ccatcgctgc
     2401 ctggtcgcgg tggccgatcg cggcaagcac cagcgcgcca gcgacgggaa caccgaggac
     2461 ggcaaggatc tcgatcactc ctcttccttc aattgctcga gatgcttga
//
